Research Topics
Genomes and Genes
Species
| epitopesSummarySummary: Sites on an antigen that interact with specific antibodies. Top Publications
Research Grants
| Scientific Experts
|
Detail Information
Publications
Broad and potent neutralizing antibodies from an African donor reveal a new HIV-1 vaccine targetLaura M Walker
Department of Immunology and Microbial Science and IAVI Neutralizing Antibody Center, Scripps Research Institute, La Jolla, CA 92037, USA
Science 326:285-9. 2009..neutralizing antibodies (bNAbs), which develop over time in some HIV-1-infected individuals, define critical epitopes for HIV vaccine design...
Rational design of envelope identifies broadly neutralizing human monoclonal antibodies to HIV-1Xueling Wu
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Science 329:856-61. 2010..Exceptionally broad HIV-1 neutralization can be achieved with individual antibodies targeted to the functionally conserved CD4bs of glycoprotein 120, an important insight for future HIV-1 vaccine design...
Antibody neutralization and escape by HIV-1Xiping Wei
Howard Hughes Medical Institute, University of Alabama at Birmingham, 720 South 20th Street, Kaul 816, Birmingham, Alabama 35294 0024, USA
Nature 422:307-12. 2003..contained mutations in the env gene that were unexpectedly sparse, did not map generally to known neutralization epitopes, and involved primarily changes in N-linked glycosylation...
Antibody recognition of a highly conserved influenza virus epitopeDamian C Ekiert
Department of Molecular Biology, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Science 324:246-51. 2009..The CR6261 epitope identified here should accelerate the design and implementation of improved vaccines that can elicit CR6261-like antibodies, as well as antibody-based therapies for the treatment of influenza...
The first T cell response to transmitted/founder virus contributes to the control of acute viremia in HIV-1 infectionNilu Goonetilleke
Medical Research Council Human Immunology Unit, Weatherall Institute of Molecular Medicine, Oxford University, Oxford OX3 9DS, England, UK
J Exp Med 206:1253-72. 2009..virus escape, these first T cell responses often rapidly waned, leaving or being succeeded by T cell responses to epitopes which escaped more slowly or were invariant...
Pre-existing immunity against swine-origin H1N1 influenza viruses in the general human populationJason A Greenbaum
Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USA
Proc Natl Acad Sci U S A 106:20365-70. 2009..we examined the molecular basis for pre-existing immunity against S-OIV, namely the recognition of viral immune epitopes by T cells or B cells/antibodies that have been previously primed by circulating influenza strains...
Structural basis for broad and potent neutralization of HIV-1 by antibody VRC01Tongqing Zhou
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health NIH, Bethesda, MD 20892, USA
Science 329:811-7. 2010..Partial receptor mimicry and extensive affinity maturation thus facilitate neutralization of HIV-1 by natural human antibodies...
Analysis of memory B cell responses and isolation of novel monoclonal antibodies with neutralizing breadth from HIV-1-infected individualsDavide Corti
Institute for Research in Biomedicine, Bellinzona, Switzerland
PLoS ONE 5:e8805. 2010....
Epochal evolution shapes the phylodynamics of interpandemic influenza A (H3N2) in humansKatia Koelle
Department of Ecology and Evolutionary Biology, 2019 Kraus Natural Science Building, University of Michigan, 830 North University Avenue, Ann Arbor, MI 48109 1048, USA
Science 314:1898-903. 2006..It captures the observed boom-and-bust pattern of viral evolution, with periods of antigenic stasis during which genetic diversity grows, and with episodic contraction of this diversity during cluster transitions...
Polyreactivity increases the apparent affinity of anti-HIV antibodies by heteroligationHugo Mouquet
Laboratory of Molecular Immunology, The Rockefeller University, New York, New York 10065, USA
Nature 467:591-5. 2010..Despite the low affinity of the polyreactive combining site, heteroligation demonstrably increases the apparent affinity of polyreactive antibodies to HIV...
Neutralizing antibody responses in acute human immunodeficiency virus type 1 subtype C infectionE S Gray
AIDS Virus Research Unit, National Institute for Communicable Diseases, Private Bag X4, Sandringham 2131, Johannesburg, South Africa
J Virol 81:6187-96. 2007..were detected in 4 of 14 individuals within a year of infection, while antibodies to CD4-induced (CD4i) epitopes developed to high titers in 12 participants, in most cases before the development of autologous neutralizing ..
Cardiolipin polyspecific autoreactivity in two broadly neutralizing HIV-1 antibodiesBarton F Haynes
Duke University School of Medicine, Durham, NC 27710, USA
Science 308:1906-8. 2005..may not induce these types of antibodies because of autoantigen mimicry of the conserved membrane-proximal epitopes of the virus...
Envelope-constrained neutralization-sensitive HIV-1 after heterosexual transmissionCynthia A Derdeyn
Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294 USA
Science 303:2019-22. 2004..Thus, the exposure of neutralizing epitopes, which are lost in chronic infection because of immune escape, appears to be favored in the newly infected host...
HIV-1 evades antibody-mediated neutralization through conformational masking of receptor-binding sitesPeter D Kwong
Vaccine Research Center, National Institutes of Health, Bethesda, Maryland 20892, USA
Nature 420:678-82. 2002..Because this solution is available for cell-surface receptors but not for most antibodies, conformational masking enables HIV-1 to maintain receptor binding and simultaneously to resist neutralization...
Predicting the evolution of human influenza AR M Bush
Department of Ecology and Evolutionary Biology, University of California, Irvine, CA 92697, USA
Science 286:1921-5. 1999..However, not all codons in these sites had predictive value. Monitoring new H3 isolates for additional changes in positively selected codons might help identify the most fit extant viral strains that arise during antigenic drift...
The role of antibodies in HIV vaccinesJohn R Mascola
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
Annu Rev Immunol 28:413-44. 2010..Thus, current vaccine design efforts have focused on a more detailed understanding of these broadly neutralizing antibodies and their epitopes to inform the design of improved vaccines.
Broadly neutralizing antibodies targeted to the membrane-proximal external region of human immunodeficiency virus type 1 glycoprotein gp41M B Zwick
Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA
J Virol 75:10892-905. 2001..Despite the apparent linear nature of the epitopes of Z13 and 4E10, denaturation of recombinant envelope protein reduces the binding of these MAbs, suggesting some ..
Structural basis of immune evasion at the site of CD4 attachment on HIV-1 gp120Lei Chen
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Science 326:1123-7. 2009..This incompatibility, the consequence of slight differences in CD4BS recognition, renders HIV-1 resistant to all but the most accurately targeted antibodies...
Broadly neutralizing human monoclonal antibodies to the hepatitis C virus E2 glycoproteinAnia M Owsianka
Institute of Virology, University of Glasgow, Church Street, Glasgow G11 5JR, UK
J Gen Virol 89:653-9. 2008..We previously isolated human monoclonal antibodies to conformational epitopes on the HCV E2 glycoprotein...
Aromatic residues at the edge of the antibody combining site facilitate viral glycoprotein recognition through membrane interactionsErin M Scherer
Department of Immunology, University of Washington, Seattle, WA 98195, USA
Proc Natl Acad Sci U S A 107:1529-34. 2010....
Antigen sensitivity is a major determinant of CD8+ T-cell polyfunctionality and HIV-suppressive activityJorge R Almeida
Institut National de la Santé et de la Recherche Médicale Unité, Hopital Pitie Salpetriere, Universite Pierre et Marie Curie, Paris, France
Blood 113:6351-60. 2009....
The immune epitope database 2.0Randi Vita
La Jolla Institute for Allergy and Immunology, Center for Infectious Disease, Allergy and Asthma Research, 9420 Athena Circle, La Jolla, CA 92037, USA
Nucleic Acids Res 38:D854-62. 2010The Immune Epitope Database (IEDB, www.iedb.org) provides a catalog of experimentally characterized B and T cell epitopes, as well as data on Major Histocompatibility Complex (MHC) binding and MHC ligand elution experiments...
Major histocompatibility complex (MHC) class II alleles, haplotypes and epitopes which confer susceptibility or protection in systemic sclerosis: analyses in 1300 Caucasian, African-American and Hispanic cases and 1000 controlsFrank C Arnett
Division of Rheumatology, Department of Internal Medicine, The University of Texas Health Science Center at Houston UTHSC H, 6431 Fannin Street, MSB 5 270, Houston, TX 77030, USA
Ann Rheum Dis 69:822-7. 2010..human leucocyte antigen-class II (HLA-class II) (DRB1, DQB1, DQA1 and DPB1) alleles, haplotypes and shared epitopes associated with scleroderma (systemic sclerosis (SSc)) and its subphenotypes in a large multi-ethnic US cohort by ..
Hepatitis C virus hypervariable region 1 modulates receptor interactions, conceals the CD81 binding site, and protects conserved neutralizing epitopesDorothea Bankwitz
Division of Experimental Virology, TWINCORE Center for Experimental and Clinical Infection Research, Feodor Lynen Strasse 7 9, 30625 Hannover, Germany
J Virol 84:5751-63. 2010..Moreover, they indicate that HVR1 obstructs the viral CD81 binding site and conserved neutralizing epitopes. These functions likely optimize virus replication, facilitate immune escape, and thus foster establishment and ..
Stable docking of neutralizing human immunodeficiency virus type 1 gp41 membrane-proximal external region monoclonal antibodies 2F5 and 4E10 is dependent on the membrane immersion depth of their epitope regionsS Moses Dennison
Human Vaccine Institute, Department of Medicine, Duke University School of Medicine, Durham, North Carolina 27710, USA
J Virol 83:10211-23. 2009..virus type 1 (HIV-1) gp41 involves both the viral membrane and gp41 membrane proximal external region (MPER) epitopes. In this study, we have used several biophysical tools to examine the secondary structure, orientation, and depth ..
Genetic impact of vaccination on breakthrough HIV-1 sequences from the STEP trialMorgane Rolland
Department of Microbiology, University of Washington, Seattle, Washington, USA
Nat Med 17:366-71. 2011..whether the vaccine exerted selective T cell pressure on breakthrough viruses, we identified potential T cell epitopes in the founder sequences and compared them to epitopes in the vaccine...
Oxidation-specific epitopes are danger-associated molecular patterns recognized by pattern recognition receptors of innate immunityYury I Miller
Department of Medicine MC0682, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
Circ Res 108:235-48. 2011..produce reactive oxygen species, which damage lipids, proteins and DNA, generating "oxidation-specific" epitopes. In this review, we discuss the hypothesis that such common oxidation-specific epitopes are a major target of ..
Structural definition of a conserved neutralization epitope on HIV-1 gp120Tongqing Zhou
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
Nature 445:732-7. 2007..A site of vulnerability, related to a functional requirement for efficient association with CD4, can therefore be targeted by antibody to neutralize HIV-1...
Prediction of continuous B-cell epitopes in an antigen using recurrent neural networkSudipto Saha
Institute of Microbial Technology, Chandigarh, India
Proteins 65:40-8. 2006B-cell epitopes play a vital role in the development of peptide vaccines, in diagnosis of diseases, and also for allergy research. Experimental methods used for characterizing epitopes are time consuming and demand large resources...
Elicitation of structure-specific antibodies by epitope scaffoldsGilad Ofek
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 107:17880-7. 2010..Epitope scaffolds thus provide a means to elicit antibodies that recognize a predetermined target shape and sequence, even if that shape is transient in nature, and a means by which to dissect factors influencing such elicitation...
NetMHC-3.0: accurate web accessible predictions of human, mouse and monkey MHC class I affinities for peptides of length 8-11Claus Lundegaard
CBS, Department of Systems Biology, Technical University of Denmark DTU, Kemitorvet Build 208, 2800 Lyngby, Denmark
Nucleic Acids Res 36:W509-12. 2008..Here, the performance is further validated and benchmarked using a large set of newly published affinity data, non-redundant to the training set. The server is free of use and available at: http://www.cbs.dtu.dk/services/NetMHC...
The neutralizing activity of anti-hepatitis C virus antibodies is modulated by specific glycans on the E2 envelope proteinFrançois Helle
Institut de Biologie de Lille UMR8161, CNRS, Université Lille I and II and Institut Pasteur de Lille, Lille, France
J Virol 81:8101-11. 2007..can limit the immunogenicity of HCV envelope proteins and restrict the binding of some antibodies to their epitopes. Here, we investigated whether these glycans can modulate the neutralizing activity of anti-HCV antibodies...
Hepatitis C virus (HCV) infection may elicit neutralizing antibodies targeting epitopes conserved in all viral genotypesNicasio Mancini
Laboratorio di Microbiologia e Virologia, Universita Vita Salute San Raffaele, Milano, Italia
PLoS ONE 4:e8254. 2009Anti-hepatitis C virus (HCV) cross-neutralizing human monoclonal antibodies, directed against conserved epitopes on surface E2 glycoprotein, are central tools for understanding virus-host interplay, and for planning strategies for ..
Production and characterization of monoclonal antibodies against different epitopes of Ebola virus antigensSoraya Shahhosseini
Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB, Canada
J Virol Methods 143:29-37. 2007..of glycoprotein (GP(1) subfragment D) were used to generate monoclonal antibodies (MAbs) against different epitopes of the viral antigens...
Quantifying influenza vaccine efficacy and antigenic distanceVishal Gupta
Departments of Bioengineering and Physics and Astronomy, Rice University, 6100 Main Street-MS 142, Houston, TX 77005-1892, USA
Vaccine 24:3881-8. 2006..We suggest that this new measure of antigenic distance be used in the design of the annual influenza vaccine and in the interpretation of vaccine efficacy monitoring...
Humoral immune responses of dengue fever patients using epitope-specific serotype-2 virus-like particle antigensWayne D Crill
Arbovirus Diseases Branch, Division of Vector Borne Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, US Department of Health and Human Service, Fort Collins, CO, USA
PLoS ONE 4:e4991. 2009..DENV-2 envelope (E) protein epitope-specific antigens and measure immunoglobulin responses to three distinct epitopes in DENV-2 infected human serum samples...
Structure and mechanistic analysis of the anti-human immunodeficiency virus type 1 antibody 2F5 in complex with its gp41 epitopeGilad Ofek
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Instiutes of Health, Bethesda, MD 20892, USA
J Virol 78:10724-37. 2004..Based on these structural and biochemical results, immunization strategies for eliciting 2F5- and 4E10-like broadly neutralizing anti-HIV-1 antibodies are proposed...
Access of antibody molecules to the conserved coreceptor binding site on glycoprotein gp120 is sterically restricted on primary human immunodeficiency virus type 1Aran F Labrijn
Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA
J Virol 77:10557-65. 2003..neutralize primary isolates is due, at least in part, to steric factors that limit antibody access to the gp120 epitopes. Studies of temperature-regulated neutralization or fusion-arrested intermediates suggest that the steric effects ..
Human combinatorial libraries yield rare antibodies that broadly neutralize hepatitis C virusDaniel X Johansson
Department of Medicine, Center for Molecular Medicine, Karolinska University Hospital Solna, Karolinska Institutet, 171 76 Stockholm, Sweden
Proc Natl Acad Sci U S A 104:16269-74. 2007..The conformational epitopes of these two broadly reactive antibodies were overlapping yet distinct and involved amino acid residues in the ..
Potent immunogenicity and efficacy of a universal influenza vaccine candidate comprising a recombinant fusion protein linking influenza M2e to the TLR5 ligand flagellinJames W Huleatt
VaxInnate Corporation, 300 George Street, New Haven, CT 06511, USA
Vaccine 26:201-14. 2008..These data demonstrate that the linkage of specific TLR ligand with influenza M2e yields a vaccine candidate that offers significant promise for widespread protection against multiple influenza A virus strains...
Gene-environment interaction between the DRB1 shared epitope and smoking in the risk of anti-citrullinated protein antibody-positive rheumatoid arthritis: all alleles are importantEmeli Lundström
Karolinska Institutet, Rheumatology Unit, Department of Medicine, Stockholm, Sweden
Arthritis Rheum 60:1597-603. 2009..We aimed to further investigate this interaction between distinct SE alleles and smoking regarding the risk of developing RA with and without anti-citrullinated protein antibodies (ACPAs)...
Antigen load and viral sequence diversification determine the functional profile of HIV-1-specific CD8+ T cellsHendrik Streeck
Partners AIDS Research Center, Infectious Disease Unit, Massachusetts General Hospital and Division of AIDS, Harvard Medical School, Boston, Massachusetts, United States of America
PLoS Med 5:e100. 2008....
The c3-v4 region is a major target of autologous neutralizing antibodies in human immunodeficiency virus type 1 subtype C infectionPenny L Moore
National Institute for Communicable Diseases, Private Bag X4, Sandringham 2131, Johannesburg, South Africa
J Virol 82:1860-9. 2008..variable alpha2-helix was not a direct target in most cases, it contributed to the formation of neutralization epitopes as substitution of this region resulted in neutralization resistance...
Epitopes involved in antibody-mediated protection from Ebola virusJ A Wilson
Virology Division, U S Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Fort Detrick, Frederick, MD 21702 5011, USA
Science 287:1664-6. 2000..We identified several protective mAbs directed toward five unique epitopes on Ebola glycoprotein...
Two functional epitopes of pigment epithelial-derived factor block angiogenesis and induce differentiation in prostate cancerStephanie Filleur
Department of Urology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA
Cancer Res 65:5144-52. 2005..We identified previously unknown antiangiogenic epitopes consisting of the 34-mer (residues 24-57) and a shorter proximal peptide (TGA, residues 16-26) with the critical ..
Genotype-specific neutralization and protection by antibodies against dengue virus type 3James D Brien
Department of Medicine, Washington University School of Medicine, 660 South Euclid Ave, Box 8051, St Louis, MO 63110, USA
J Virol 84:10630-43. 2010..Using yeast surface display, we localized the epitopes of the most strongly neutralizing MAbs to the lateral ridge of domain III (DIII) of the DENV type 3 (DENV-3) E ..
Epitope-specific evolution of human CD8(+) T cell responses from primary to persistent phases of Epstein-Barr virus infectionAndrew D Hislop
CRC Institute for Cancer Studies, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom
J Exp Med 195:893-905. 2002..Using HLA-A2.1 or B8 tetramers specific for four lytic cycle and three latent cycle epitopes, we find marked differences in the epitope-specific composition of the T cell populations between the two phases ..
Combination of two insulin-like growth factor-I receptor inhibitory antibodies targeting distinct epitopes leads to an enhanced antitumor responseJianying Dong
Department of Discovery Oncology, Biogen Idec, Inc, San Diego, California 92122, USA
Mol Cancer Ther 9:2593-604. 2010..We identified a spectrum of inhibitory IGF-IR antibodies with diverse binding epitopes and ligand-blocking properties...
Induction of epitope-specific neutralizing antibodies against West Nile virusTheodore Oliphant
Departments of Medicine, Molecular Microbiology, and Pathology and Immunology, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, MO 63110, USA
J Virol 81:11828-39. 2007..virus particles, we evaluated the expression pattern and activity of antibodies against the DIII-lr and DII-fl epitopes in mouse and human serum after WNV infection...
Simultaneous detection of circulating autoreactive CD8+ T-cells specific for different islet cell-associated epitopes using combinatorial MHC multimersJurjen H Velthuis
Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands
Diabetes 59:1721-30. 2010..Yet, conventional detection techniques (ELISPOT and HLA tetramers) require many cells and are relatively insensitive...
Divergent adaptation of hepatitis C virus genotypes 1 and 3 to human leukocyte antigen-restricted immune pressureAndri Rauch
University Clinic of Infectious Diseases, University Hospital Bern and University of Bern, Bern, Switzerland
Hepatology 50:1017-29. 2009..are known to be important in the containment of HCV genotype 1 infection, and many genotype 1 T cell targets (epitopes) that are presented by host human leukocyte antigens (HLAs) have been identified...
Monoclonal antibody AP33 defines a broadly neutralizing epitope on the hepatitis C virus E2 envelope glycoproteinAnia Owsianka
MRC Virology Unit, Institute of Virology, University of Glasgow, UK
J Virol 79:11095-104. 2005..Thus, identification of a broadly neutralizing antibody that recognizes a linear epitope is likely to be of significant benefit to future vaccine and therapeutic antibody development...
Neutralization of West Nile virus by cross-linking of its surface proteins with Fab fragments of the human monoclonal antibody CR4354Bärbel Kaufmann
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907 2054, USA
Proc Natl Acad Sci U S A 107:18950-5. 2010....
Glycogen synthase kinase 3 beta is identical to tau protein kinase I generating several epitopes of paired helical filamentsK Ishiguro
Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan
FEBS Lett 325:167-72. 1993..Moreover, we found that TPKI activity was inseparable from GSK3 activity throughout the purification procedure. These results indicate that TPKI is identical to GSK3 beta...
Isolation of human monoclonal antibodies that potently neutralize human cytomegalovirus infection by targeting different epitopes on the gH/gL/UL128-131A complexAnnalisa Macagno
Institute for Research in Biomedicine, Via Vincenzo Vela 6, CH 6500 Bellinzona, Switzerland
J Virol 84:1005-13. 2010..that bound to a conserved epitope in the UL128 gene product, all other antibodies bound to conformational epitopes that required expression of two or more proteins of the gH/gL/UL128-131A complex...
Measurement of phosphorylated tau epitopes in the differential diagnosis of Alzheimer disease: a comparative cerebrospinal fluid studyHarald Hampel
Dementia Research Section and Memory Clinic, Alzheimer Memorial Center and Geriatric Psychiatry Branch, Department of Psychiatry, Ludwig Maximilian University, Munich, Germany
Arch Gen Psychiatry 61:95-102. 2004..Different tau phosphoepitopes can be sensitively detected in cerebrospinal fluid (CSF)...
Conserved epitopes of influenza A virus inducing protective immunity and their prospects for universal vaccine developmentZuzana Staneková
Institute of Virology, Slovak Academy of Sciences, Dubravska cesta 9, 845 05 Bratislava, Slovak Republic
Virol J 7:351. 2010..Combination of chosen conserved antigens inducing cross-protective antibody response with epitopes activating also cross-protective cytotoxic T-cells would offer an attractive strategy for improving protection ..
Chondroitin sulfate A-adhering Plasmodium falciparum-infected erythrocytes express functionally important antibody epitopes shared by multiple variantsLea Barfod
Centre for Medical Parasitology, Department of International Health, Immunology, and Microbiology, University of Copenhagen, Denmark
J Immunol 185:7553-61. 2010..gene is lower than that among var genes in general, VAR2CSA-specific Abs appear to target mainly polymorphic epitopes. This has raised doubts about the feasibility of VAR2CSA-based vaccines...
Efficient targeting of conserved cryptic epitopes of infectious agents by single domain antibodies. African trypanosomes as paradigmBenoit Stijlemans
Department of Cellular and Molecular Interactions, Vlaams Interuniversitair Instituut voor Biotechnologie, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussels, Belgium
J Biol Chem 279:1256-61. 2004..involves a dense packing of variant surface glycoproteins (VSG) exposing only highly variable and immuno-dominant epitopes to the immune system, whereas conserved epitopes become inaccessible for large molecules...
Heat shock protein 60: identification of specific epitopes for binding to primary macrophagesChristiane Habich
German Diabetes Clinic, German Diabetes Center, Leibniz Institute, Heinrich Heine University of Dusseldorf, D 40225 Dusseldorf, Germany
FEBS Lett 580:115-20. 2006..Analysis of different pro- and eukaryotic HSP60 species indicated that phylogenetically separate HSP60 species use different binding sites on primary macrophages...
Evidence for globally shared, cross-reacting polymorphic epitopes in the pregnancy-associated malaria vaccine candidate VAR2CSAMarion Avril
Seattle Biomedical Research Institute, 307 Westlake Ave N, Suite 500, Seattle, WA 98109 5219, USA
Infect Immun 76:1791-800. 2008..Antibodies did not target conserved epitopes exposed in all VAR2CSA alleles; however, antisera to several DBL domains cross-reacted on parasite isolates that ..
Epitope identification and discovery using phage display libraries: applications in vaccine development and diagnosticsLin Fa Wang
CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong 3220, Victoria, Australia
Curr Drug Targets 5:1-15. 2004Antigenic epitopes are the part (contact points) of an antigen involved in specific interaction with the antigen-binding site (the paratope) of an antibody or a T-cell receptor...
In vitro assay for neutralizing antibody to hepatitis C virus: evidence for broadly conserved neutralization epitopesBirke Bartosch
, , IFR 128, , , 69364 Lyon Cedex 07, France
Proc Natl Acad Sci U S A 100:14199-204. 2003..directed not only to epitope(s) in the hypervariable region of the E2 envelope protein but also to one or more epitopes elsewhere in the envelope of the virus...
Reverse vaccinology: developing vaccines in the era of genomicsAlessandro Sette
La Jolla Institute for Allergy and Immunology, San Diego, CA 92130, USA
Immunity 33:530-41. 2010..targets in bacteria, parasites, and large viruses and revealed virtually all their CD4(+) and CD8(+) T cell epitopes. The genomic information was first used for the development of a vaccine against serogroup B meningococcus, and ..
In vivo gp41 antibodies targeting the 2F5 monoclonal antibody epitope mediate human immunodeficiency virus type 1 neutralization breadthXiaoying Shen
Duke Human Vaccine Institute, Duke University Medicine Center, Durham, NC 27710, USA
J Virol 83:3617-25. 2009..serum antibodies from an HIV-infected subject that both were broadly neutralizing and specifically targeted MPER epitopes that overlap the 2F5 epitope...
Computational design of epitope-scaffolds allows induction of antibodies specific for a poorly immunogenic HIV vaccine epitopeBruno E Correia
Department of Biochemistry, University of Washington, Seattle, WA 98195, USA
Structure 18:1116-26. 2010Broadly cross-reactive monoclonal antibodies define epitopes for vaccine development against HIV and other highly mutable viruses...
Molecular characterization of antibody epitopes of Ehrlichia chaffeensis ankyrin protein 200 and tandem repeat protein 47 and evaluation of synthetic immunodeterminants for serodiagnosis of human monocytotropic ehrlichiosisTian Luo
Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555 0609, USA
Clin Vaccine Immunol 17:87-97. 2010Recently, major species-specific antibody epitopes in three immunoreactive tandem repeat proteins (TRPs) of Ehrlichia chaffeensis, TRP32, TRP47, and TRP120, have been identified and molecularly characterized within tandem repeat (TR) ..
Opposing effects of HLA-DRB1*13 alleles on the risk of developing anti-citrullinated protein antibody-positive and anti-citrullinated protein antibody-negative rheumatoid arthritisEmeli Lundström
Karolinska Institutet, Stockholm, Sweden
Arthritis Rheum 60:924-30. 2009....
Identification of human T cell receptor gammadelta-recognized epitopes/proteins via CDR3delta peptide-based immunobiochemical strategyHui Chen
Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Peking Union Medical College, National Key Laboratory of Medical Molecular Biology, Beijing 100005, China
J Biol Chem 283:12528-37. 2008..However, few tumor antigens recognized by TCRgammadelta have been defined so far. To investigate antigenic epitopes/proteins recognized by gammadeltaT cells, we have established a new immunobiochemical strategy that uses ..
An HPV 16 L1-based chimeric human papilloma virus-like particles containing a string of epitopes produced in plants is able to elicit humoral and cytotoxic T-cell activity in miceGeorgina Paz De la Rosa
Centro de Investigación y de Estudios Avanzados Cinvestav, Unidad Irapuato, km, 9, 6 libramiento norte, 36500 Carretera Irapuato León, Irapuato, Guanajuato, Mexico
Virol J 6:2. 2009..Several HPV genes have been expressed in plants, including L1, which can self-assemble into virus-like particles. A plant-based, dual prophylactic/therapeutic vaccine remains an attractive possibility...
A comprehensive toolkit of plant cell wall glycan-directed monoclonal antibodiesSivakumar Pattathil
Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia 30602, USA
Plant Physiol 153:514-25. 2010..The mAbs could be resolved into 19 clades of antibodies that recognize distinct epitopes present on all major classes of plant cell wall glycans, including arabinogalactans (both protein- and ..
Identification of an H-2D(b)-restricted CD8+ cytotoxic T lymphocyte epitope in the matrix protein of respiratory syncytial virusJohn A Rutigliano
Vaccine Research Center, NIAID, National Institutes of Health, Building 40, Room 2502, 40 Convent Drive, MSC 3017, Bethesda, MD 20892-3017, USA
Virology 337:335-43. 2005..Identification of class I MHC-restricted CTL epitopes is critical in elucidating mechanisms of CTL responses against viral infections...
The biological activity of human CD20 monoclonal antibodies is linked to unique epitopes on CD20Jessica L Teeling
Genmab, Utrecht, The Netherlands
J Immunol 177:362-71. 2006..We conclude that recognition of the novel epitope cooperates with slow off-rate in determining the activity of CD20 Ab in activation of complement and induction of tumor cell lysis...
Hepatitis C virus mutation affects proteasomal epitope processingUlrike Seifert
Institute of Biochemistry, Charit, Humboldt University, Berlin, Germany
J Clin Invest 114:250-9. 2004....
Epitope analysis for influenza vaccine designEnrique T Munoz
Department of Bioengineering and Department of Physics and Astronomy, Rice University Houston, TX 77005 1892, USA
Vaccine 23:1144-8. 2005..from statistical mechanics to quantify the non-monotonic immune response that results from antigenic drift in the epitopes of the hemagglutinin and neuraminidase proteins...
Cancer biomarkers defined by autoantibody signatures to aberrant O-glycopeptide epitopesHans H Wandall
Center for Glycomics, Departments of Cellular and Molecular Medicine and Oral Diagnostics, University of Copenhagen, Blegdamsvej 3, Copenhagen 2200 N, Denmark
Cancer Res 70:1306-13. 2010..An O-glycopeptide microarray was developed that detected IgG antibodies to aberrant O-glycopeptide epitopes in patients vaccinated with a keyhole limpet hemocyanin-conjugated truncated MUC1 peptide...
Characterization of epitopes for neutralizing monoclonal antibodies to classical swine fever virus E2 and Erns using phage-displayed random peptide libraryF Zhang
CSIRO Livestock Industries, Australian Animal Health Laboratory, Geelong, Australia
Arch Virol 151:37-54. 2006..Here we report the identification of multiple neutralizing epitopes on these proteins by screening a phage-displayed random peptide library with CSFV-specific neutralizing ..
Recognition of variant HIV-1 epitopes from diverse viral subtypes by vaccine-induced CTLDenise M McKinney
Epimmune, San Diego, CA 92121, USA
J Immunol 173:1941-50. 2004Recognition by CD8(+) T lymphocytes (CTL) of epitopes that are derived from conserved gene products, such as Gag and Pol, is well documented and conceptually supports the development of epitope-based vaccines for use against diverse HIV-1 ..
Phage display for epitope determination: a paradigm for identifying receptor-ligand interactionsMerrill J Rowley
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia
Biotechnol Annu Rev 10:151-88. 2004..of protein and non-protein nature are widely studied in biology and have particular utilities, but the precise epitopes recognized are seldom well defined...
Cryptic nature of a conserved, CD4-inducible V3 loop neutralization epitope in the native envelope glycoprotein oligomer of CCR5-restricted, but not CXCR4-using, primary human immunodeficiency virus type 1 strainsPaolo Lusso
Unit of Human Virology, Department of Biological and Technological Research DIBIT, San Raffaele Scientific Institute, Via Olgettina 58, 20132 Milan, Italy
J Virol 79:6957-68. 2005..For the conserved neutralization epitopes hitherto described, antibody accessibility is reportedly unrelated to the viral coreceptor usage phenotype...
An antibody produced in tobacco expressing a hybrid beta-1,4-galactosyltransferase is essentially devoid of plant carbohydrate epitopesHans Bakker
Business Unit Bioscience, Plant Research International, Wageningen University and Research Center, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands
Proc Natl Acad Sci U S A 103:7577-82. 2006..Hence, a transgenic plant expressing the hybrid GalT might yield more effective and safer monoclonals for therapeutic purposes than WT plants and even transgenic plants expressing the unchanged GalT...
Immunization of mice with human 60-kd Ro peptides results in epitope spreading if the peptides are highly homologous between human and mouseR H Scofield
Oklahoma Medical Research Foundation, University of Oklahoma Health Sciences Center, Department of Veterans Affairs Medical Center, and WK Warren Medical Research Institute, Oklahoma City 73104, USA
Arthritis Rheum 42:1017-24. 1999..The purpose of this study was to determine the ability of several different peptides from 60-kd Ro to induce expansion of the immune response to the Ro/La RNP particle...
Colocalization of tau and alpha-synuclein epitopes in Lewy bodiesTakashi Ishizawa
Department of Pathology (Neuropathology, Mayo Clinic, 4500 San Pablo Road, Jacksonville, FL 32224, USA
J Neuropathol Exp Neurol 62:389-97. 2003..rabbit polyclonal antibody to alpha-synuclein and a panel of monoclonal antibodies to phospho- and nonphospho-tau epitopes (Alz50, CP9, CP13, PG5, TG3, PHFI) that spanned the length of the tau molecule...
Production of a monoclonal antibody in plants with a humanized N-glycosylation patternMatthias Schähs
Institute of Applied Genetics and Cell Biology, BOKU Wien, Austria
Plant Biotechnol J 5:657-63. 2007..the presence of beta1,2-xylose and core alpha1,3-fucose residues on complex N-linked glycans, as these N-glycan epitopes are immunogenic in mammals...
Kinetics of expansion of epitope-specific T cell responses during primary HIV-1 infectionEmma L Turnbull
Viral Immunology Group, Jenner Institute, University of Oxford, Compton, United Kingdom
J Immunol 182:7131-45. 2009..These observations document the preferential expansion of CD8(+) T cells recognizing a subset of epitopes during the viral burst in acute HIV-1 infection and suggest that the nature of the initial, very rapidly expanded ..
Synthetic glycopeptide vaccines combining beta-mannan and peptide epitopes induce protection against candidiasisHong Xin
Department of Pediatrics, Louisiana State University Health Sciences Center and Research Institute for Children, Children s Hospital, New Orleans, LA 70118, USA
Proc Natl Acad Sci U S A 105:13526-31. 2008..This approach based on fully synthetic chemically defined immunogens should be generally useful in vaccine development...
Design and pre-clinical evaluation of a universal HIV-1 vaccineSven Létourneau
Medical Research Council Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, The John Radcliffe, Oxford, United Kingdom
PLoS ONE 2:e984. 2007..One of the big roadblocks in development of HIV-1/AIDS vaccines is the enormous diversity of HIV-1, which could limit the value of any HIV-1 vaccine candidate currently under test...
Force-dependent polymorphism in type IV pili reveals hidden epitopesNicolas Biais
Department of Biological Sciences, Columbia University, 1212 Amsterdam Avenue, New York, NY 10027, USA
Proc Natl Acad Sci U S A 107:11358-63. 2010..Equally important, we show that the force-induced conformation exposes hidden epitopes previously buried in the Tfp fiber. We postulate that this transition provides a means for N...
Conserved structural elements in the V3 crown of HIV-1 gp120Xunqing Jiang
Department of Biochemistry, New York University School of Medicine, New York, New York, USA
Nat Struct Mol Biol 17:955-61. 2010..These conserved elements could serve as epitopes to be targeted by a vaccine against HIV-1...
Glycomic analysis of N-linked carbohydrate epitopes from CD24 of mouse brainChristina Bleckmann
Institute of Biochemistry, University of Giessen, Friedrichstrasse 24, D 35392 Giessen, Germany
J Proteome Res 8:567-82. 2009..Our data revealed a highly heterogeneous pattern of mainly complex type glycans expressing distinct carbohydrate epitopes, like 3-linked sialic acid, Le(X) or blood group H antigens, bisecting N-acetylglucosamine residues and N-..
Lipid transfer proteins from Rosaceae fruits share consensus epitopes responsible for their IgE-binding cross-reactivityJean Philippe Borges
Surfaces Cellulaires et Signalisation chez les Végétaux, UMR Université Paul Sabatier CNRS 5546, 24 Chemin de Borde Rouge, 31326 Castanet Tolosan, France
Biochem Biophys Res Commun 365:685-90. 2008Four IgE-binding epitopes have been characterized that cover a large area (40%) of the molecular surface of lipid transfer protein allergens of Rosaceae (apple, peach, apricot, and plum)...
Detection of apoptosis in vivo using antibodies against caspase-induced neo-epitopesSabine Jakob
Division of Immunopathology, Institute of Pathology, University of Bern, Murtenstrasse 31, 3010 Bern, Switzerland
Methods 44:255-61. 2008..Furthermore, we evaluate the effect of tissue pretreatment and antigen retrieval on the sensitivity of apoptosis detection, background staining and maintenance of tissue morphology...
Neutralizing monoclonal antibodies against hepatitis C virus E2 protein bind discontinuous epitopes and inhibit infection at a postattachment stepMichelle C Sabo
Department of Pathology and Immunology, Washington University School of Medicine, 660 South Euclid Ave, Box 8051, Saint Louis, MO 63110, USA
J Virol 85:7005-19. 2011..16 blocked attachment to SR-B1 only. Using yeast surface display, we localized epitopes for the neutralizing MAbs on the E2 protein. Two of the strongly inhibitory MAbs, H77.16 and J6...
REMUS: a tool for identification of unique peptide segments as epitopesTun Wen Pai
Department of Computer Science and Engineering, National Taiwan Ocean University, Keelung, Taiwan, Republic of China
Nucleic Acids Res 34:W198-201. 2006..the amino acids, frequency of appearance and biological properties such as priority for serving as candidates for epitopes where antibodies recognize...
Characterisation of the transcription factor, SIX5, using a new panel of monoclonal antibodiesY Chan N Pham
Biochemistry Group, North East Wales Institute, Mold Road, Wrexham, United Kingdom
J Cell Biochem 95:990-1001. 2005..We have produced a new panel of 18 monoclonal antibodies (mAbs) that recognise at least four different epitopes in order to identify authentic human SIX5 protein in cells and tissues...
A mouse monoclonal antibody to the TSHRHanna Stankowiak-Kulpa
FIRS Laboratories, RSR Ltd, Llanishen, Cardiff, UK
Arch Immunol Ther Exp (Warsz) 53:345-51. 2005..The 3C3 mAb we produced was found to inhibit binding of TSH to human (h)TSHR but not to porcine (p)TSHR...
Immune responses of selected phagotopes from monoclonal antibodies of Burkholderia pseudomalleiNarisorn Na-ngam
Department of Veterinary Medicine, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen, Thailand
Southeast Asian J Trop Med Public Health 39:443-51. 2008..The study demonstrates the feasibility of identifying mimotopes through screening of phage-displayed random peptide libraries with B. pseudomallei MAbs...
Characterization of cross-reactive and serotype-specific epitopes on the nucleocapsid proteins of hantavirusesNicole D Tischler
Fundación Ciencia para la Vida, Nunoa, Santiago, Chile
Virus Res 135:1-9. 2008..Here we report on epitopes involved in serotype-specific and cross-reactive recognition of the N proteins of hantaviruses using monoclonal ..
Molecular basis for the binding polyspecificity of an anti-cholera toxin peptide 3 monoclonal antibodyLivia Otte
Institut fur Medizinische Immunologie, Charite Universitatsmedizin Berlin, Berlin, Germany
J Mol Recognit 19:49-59. 2006..However, the overall binding conformation of the peptides is similar. Together, our results support the theory of a general polyspecific potential of mAbs...
Conformation-specific antibodies reveal distinct actin structures in the nucleus and the cytoplasmC A Schoenenberger
M E Müller Institute for Structural Biology, Biozentrum, University of Basel, CH 4056 Basel, Switzerland
J Struct Biol 152:157-68. 2005..Our results indicate that distinct actin conformations and/or structures are present in the nucleus and the cytoplasm of different cell types and that their distribution varies in response to external signals...
Characterization and application of monoclonal antibodies against human endothelin B receptor expressed in insect cellsTomohiro Yamaguchi
Department of Biophysics, Graduate School of Science, Kyoto University, Oiwake, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan
Biotechnol Lett 26:293-9. 2004..Five typical monoclonal antibodies were characterized further for their recognition. The epitopes for the 2A5, 9A3 and 21A1 antibodies were mapped within the N-terminal extracellular sequences, V71-I85 and E27-..
Research Grants
- Brucella epitope recognition by CD8+ T cellsJEROME SCOTT HARMS; Fiscal Year: 2010..Our goal is to identify specific Brucella CD8+ T cell epitopes and test their ability to protect mice against infection with virulent Brucella...
- Brucella epitope recognition by CD8+ T cellsGary Splitter; Fiscal Year: 2009..Our goal is to identify specific Brucella CD8+ T cell epitopes and test their ability to protect mice against infection with virulent Brucella...
- SPOTTED FEVER RICKETTSIAL ANTIGENSDavid Walker; Fiscal Year: 2003..The specific aims of this project will be to: 1) define the dominant CD8 T-lymphocyte epitopes of rOmpA and rOmpB of Rickettsia conorii at the oligopeptide level and identify the epitopes processed ..
- VARIABLE GENE USE AND PNEUMOCOCCAL IMMUNITY IN AIDSLiise Anne Pirofski; Fiscal Year: 2004..3 ) To use Mabs with functional efficacy as defined in aim two to isolate peptide mimetics of PPS 8 and 23F epitopes, and to use the peptides to determine if PPS-elicited antibodies in patients sera recognize protective, non-..
- CD8+ T cell specificity in mouse models of smallpox vaccination and challengeDavid Tscharke; Fiscal Year: 2007..Recently, several CD8+ T cell epitopes of VACV have been identified in mice, some of which are shared with ectromelia virus (ECTV)...
- Dengue Epitope Vaccine,Tetravalent & MHCII-TargetedJ Thomas August; Fiscal Year: 2007..of a new form of epitope-based dengue genetic vaccine encoding an ensemble of selected antigen peptide units (epitopes) containing the minimum antibody and cellular antigen sequences required for an effective vaccine applicable to ..
- GENE THERAPEUTIC APPROACH FOR TOLERANCE INDUCTIONDAVID WILLIAM SCOTT; Fiscal Year: 2010..last decade, we created a platform technology that can be used for gene therapy to induce tolerance to multiple epitopes. In our model, LPS-activated B cells are transduced via retroviral vectors to express an Ig fusion protein with ..
- GENE THERAPEUTIC APPROACH FOR TOLERANCE INDUCTIONDavid Scott; Fiscal Year: 2009..last decade, we created a platform technology that can be used for gene therapy to induce tolerance to multiple epitopes. In our model, LPS-activated B cells are transduced via retroviral vectors to express an Ig fusion protein with ..
- GENE THERAPEUTIC APPROACH FOR TOLERANCE INDUCTIONDavid Scott; Fiscal Year: 2009..last decade, we created a platform technology that can be used for gene therapy to induce tolerance to multiple epitopes. In our model, LPS-activated B cells are transduced via retroviral vectors to express an Ig fusion protein with ..
- IMMUNOREGULATION AND PATHOLOGY OF CHRONIC-RELAPSING EAEStephen Miller; Fiscal Year: 2004..following active immunization with proteolipid protein (PLP), myelin basic protein (MBP), or the immunodominant epitopes on these molecules (PLP139-151 or MBP84-104) or following the adoptive transfer of peptide-specific Th1 cells...
- CC Chemokine Secretion to Protect Antigen-Specific CD4 T Cells from HIVCatarina E Hioe; Fiscal Year: 2010..Antigen stimulation of CD4 T cells specific for certain HIV epitopes have been shown to render these cells more resistant to CCR5-tropic HIV and this resistance is associated with ..
- Human Autoantibodies in Anti-GBM DiseaseAnne Christensen; Fiscal Year: 2005..Although putative antigenic epitopes have been identified, little is known about the origin, structure and conformation of pathogenic human anti-GBM ..
- Novel chimeric Env vaccines to elicit broadly neutralizing antibodies against HIVLing Ye; Fiscal Year: 2010..hindrance exerted by the large, hypervariable, and heavily glycosylated gp120 subunit on conserved neutralizing epitopes. The gp41 subunit is less variable than gp120, and has been shown to contain conserved neutralizing epitopes such ..
- Xenograft-like rejection of tumors in a-gal glycolipidsURI GALILI GALILI; Fiscal Year: 2010..them into endogenous tumor vaccines by intratumoral injection of micelles of glycosphingolipids bearing a-gal epitopes (Gala1-3Galb1-4GlcNAc-R) (designated GSL?gal)...
- Protective Human Monoclonal Antibodies to HIV-1SUSAN B ZOLLA PAZNER; Fiscal Year: 2010..to be "neutralizing Abs" that have been defined by studies of several human monoclonal Abs (mAbs) as targeting epitopes in the membrane proximal external region of gp41 and in various regions of gp120 including the CD4 binding site, ..
- Novel Linear Peptide Universal Malaria VaccinesAlberto Moreno; Fiscal Year: 2005..is to evaluate the potential of synthetic linear peptide chimeras (LPCs) containing Plasmodium universal T cell epitopes and immunodominant B cell epitopes as effective malaria vaccine components...
- Molecular Analysis of FIVJohn Elder; Fiscal Year: 2007..The Specific Aims of the proposal are to 1) Map binding epitopes of a panel of monoclonal antibodies that recognize distinct regions of the FIV envelope glycoprotein...
- THYROID PEROXIDASE AS AN AUTOANTIGENBasil Rapoport; Fiscal Year: 2005..We plan the following specific studies:- 1. TPO AUTOANTIBODY EPITOPES Polyclonal TPO autoantibodies in patients' sera are primarily directed to a restricted immunodominant region (IDR)..
- Immune Recognition of Modified Antigen in SclerodermaRobert Hoffman; Fiscal Year: 2003..We hypothesize that oxidative modified 70k exposes previously cryptic epitopes, driving the development of scleroderma-associated anti-70k immune response...
