escherichia coli adhesins

Summary

Summary: Thin, filamentous protein structures, including proteinaceous capsular antigens (fimbrial antigens), that mediate adhesion of E. coli to surfaces and play a role in pathogenesis. They have a high affinity for various epithelial cells.

Top Publications

  1. ncbi Exploring the 3D molecular architecture of Escherichia coli type 1 pili
    Erik Hahn
    Institute of Veterinary Anatomy, University of Zurich, Switzerland
    J Mol Biol 323:845-57. 2002
  2. ncbi Escherichia coli strains belonging to phylogenetic group B2 have superior capacity to persist in the intestinal microflora of infants
    Forough L Nowrouzian
    Department of Clinical Bacteriology, Goteborg University, Goteborg, Sweden
    J Infect Dis 191:1078-83. 2005
  3. pmc Chaperone-subunit-usher interactions required for donor strand exchange during bacterial pilus assembly
    Michelle M Barnhart
    Department of Molecular Microbiology, Washington University School of Medicine, St Louis, Missouri 63110 1093, USA
    J Bacteriol 185:2723-30. 2003
  4. ncbi Structural basis of tropism of Escherichia coli to the bladder during urinary tract infection
    Chia Suei Hung
    Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110, USA
    Mol Microbiol 44:903-15. 2002
  5. doi Clathrin, AP-2, and the NPXY-binding subset of alternate endocytic adaptors facilitate FimH-mediated bacterial invasion of host cells
    Danelle S Eto
    Division of Cell Biology and Immunology, Pathology Department, University of Utah, Salt Lake City, UT 84112 0565, USA
    Cell Microbiol 10:2553-67. 2008
  6. pmc Covert operations of uropathogenic Escherichia coli within the urinary tract
    Jean M Bower
    Pathology Department, Division of Cell Biology and Immunology, University of Utah School of Medicine, 30 North 1900 East, Salt Lake City, Utah 84132 2501, USA
    Traffic 6:18-31. 2005
  7. ncbi Recognition of the cellular beta1-chain integrin by the bacterial AfaD invasin is implicated in the internalization of afa-expressing pathogenic Escherichia coli strains
    Laure Plançon
    Unité de Pathogénie Bactérienne des Muqueuses, Institut Pasteur 28 rue du Dr Roux, F 75724 Paris, France
    Cell Microbiol 5:681-93. 2003
  8. pmc Tight modulation of Escherichia coli bacterial biofilm formation through controlled expression of adhesion factors
    Sandra Da Re
    Groupe de Génétique des Biofilms, CNRS URA 2172, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France
    Appl Environ Microbiol 73:3391-403. 2007
  9. ncbi Bacterial adhesion to target cells enhanced by shear force
    Wendy E Thomas
    Department of Bioengineering, 98195, Seattle, WA, USA
    Cell 109:913-23. 2002
  10. ncbi Sequence analysis demonstrates the conservation of fimH and variability of fimA throughout avian pathogenic Escherichia coli (APEC)
    Fréderic Vandemaele
    Laboratory of Physiology and Immunology of Domestic Animals, Catholic University Leuven, Kasteelpark Arenberg 30, 3001 Leuven, Belgium
    Vet Res 34:153-63. 2003

Research Grants

  1. CD556, Infection, and Race in Preterm Delivery
    Stella Nowicki; Fiscal Year: 2005
  2. CD556, Infection, and Race in Preterm Delivery
    Bogdan Nowicki; Fiscal Year: 2004
  3. Dynamic Properties of Bacterial Adhesions
    Evgeni Sokurenko; Fiscal Year: 2005
  4. Pathoadaptive evolution of Salmonella
    Evgeni Sokurenko; Fiscal Year: 2009
  5. Infectious Disease Training in Clinical Investigation
    James Johnson; Fiscal Year: 2007
  6. PATHOGENIC ADAPTATION OF MICROBIAL ADHESINS
    Evgeni Sokurenko; Fiscal Year: 2009
  7. Dynamic Properties of Bacterial Adhesins
    Evgeni Sokurenko; Fiscal Year: 2007
  8. Resistant E.coli in Humans and Poultry
    James Johnson; Fiscal Year: 2005
  9. Pathoadaptive evolution of Salmonella
    EVGENI VENIAMINOVIC contact SOKURENKO; Fiscal Year: 2010
  10. PATHOGENIC ADAPTATION OF MICROBIAL ADHESINS
    Evgeni Sokurenko; Fiscal Year: 2007

Detail Information

Publications152 found, 100 shown here

  1. ncbi Exploring the 3D molecular architecture of Escherichia coli type 1 pili
    Erik Hahn
    Institute of Veterinary Anatomy, University of Zurich, Switzerland
    J Mol Biol 323:845-57. 2002
    ....
  2. ncbi Escherichia coli strains belonging to phylogenetic group B2 have superior capacity to persist in the intestinal microflora of infants
    Forough L Nowrouzian
    Department of Clinical Bacteriology, Goteborg University, Goteborg, Sweden
    J Infect Dis 191:1078-83. 2005
    ..03). Thus, group B2 strains appear to possess yet unidentified traits that enhance their survival in the human intestine...
  3. pmc Chaperone-subunit-usher interactions required for donor strand exchange during bacterial pilus assembly
    Michelle M Barnhart
    Department of Molecular Microbiology, Washington University School of Medicine, St Louis, Missouri 63110 1093, USA
    J Bacteriol 185:2723-30. 2003
    ..Furthermore, the donor strand exchange assembly mechanism between subunits was found to be necessary for subunit translocation across the outer membrane usher...
  4. ncbi Structural basis of tropism of Escherichia coli to the bladder during urinary tract infection
    Chia Suei Hung
    Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110, USA
    Mol Microbiol 44:903-15. 2002
    ..coli (EHEC) possess a sequence variation within the mannose-binding pocket of FimH, suggesting a naturally occurring mechanism of attenuation in EHEC bacteria that would prevent them from being targeted to the urinary tract...
  5. doi Clathrin, AP-2, and the NPXY-binding subset of alternate endocytic adaptors facilitate FimH-mediated bacterial invasion of host cells
    Danelle S Eto
    Division of Cell Biology and Immunology, Pathology Department, University of Utah, Salt Lake City, UT 84112 0565, USA
    Cell Microbiol 10:2553-67. 2008
    ....
  6. pmc Covert operations of uropathogenic Escherichia coli within the urinary tract
    Jean M Bower
    Pathology Department, Division of Cell Biology and Immunology, University of Utah School of Medicine, 30 North 1900 East, Salt Lake City, Utah 84132 2501, USA
    Traffic 6:18-31. 2005
    ..Here we review the diverse mechanisms and consequences of host cell invasion by UPEC, focusing also on the impact of these processes on the persistence and recurrence of UTIs...
  7. ncbi Recognition of the cellular beta1-chain integrin by the bacterial AfaD invasin is implicated in the internalization of afa-expressing pathogenic Escherichia coli strains
    Laure Plançon
    Unité de Pathogénie Bactérienne des Muqueuses, Institut Pasteur 28 rue du Dr Roux, F 75724 Paris, France
    Cell Microbiol 5:681-93. 2003
    ..Our results suggest that beta1 integrin is a common receptor for AfaD invasins and that additional AfaD-type-specific receptors exist...
  8. pmc Tight modulation of Escherichia coli bacterial biofilm formation through controlled expression of adhesion factors
    Sandra Da Re
    Groupe de Génétique des Biofilms, CNRS URA 2172, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France
    Appl Environ Microbiol 73:3391-403. 2007
    ..coli or those of other bacteria capable of being genetically manipulated may be of use both for reducing and for improving the impact of biofilms in a number of industrial and medical applications...
  9. ncbi Bacterial adhesion to target cells enhanced by shear force
    Wendy E Thomas
    Department of Bioengineering, 98195, Seattle, WA, USA
    Cell 109:913-23. 2002
    ..The ability of FimH to function as a force sensor provides a molecular mechanism for discrimination between surface-exposed and soluble receptor molecules...
  10. ncbi Sequence analysis demonstrates the conservation of fimH and variability of fimA throughout avian pathogenic Escherichia coli (APEC)
    Fréderic Vandemaele
    Laboratory of Physiology and Immunology of Domestic Animals, Catholic University Leuven, Kasteelpark Arenberg 30, 3001 Leuven, Belgium
    Vet Res 34:153-63. 2003
    ..This study elucidated the molecular structure and the degree of conservation of FimH and FimA among various avian pathogenic E. coli strains...
  11. ncbi Molecular structure of adhesin domains in Escherichia coli fimbriae
    Benita Westerlund-Wikström
    General Microbiology, Department of Biological and Environmental Sciences, Faculty of Biosciences, FIN 00014 University of Helsinki, Finland
    Int J Med Microbiol 295:479-86. 2005
    ..The existing structures give insight into the molecular basis of the diversity in fimbrial lectins...
  12. ncbi X-ray structure of the FimC-FimH chaperone-adhesin complex from uropathogenic Escherichia coli
    D Choudhury
    Department of Molecular Biology, Uppsala Biomedical Center, Swedish University of Agricultural Sciences, Box 590, S 753 24 Uppsala, Sweden
    Science 285:1061-6. 1999
    ..A donor strand complementation mechanism in which the chaperone donates a strand to complete the pilin domain explains the basis for both chaperone function and pilus biogenesis...
  13. ncbi Avian pathogenic Escherichia coli (APEC)
    M Dho-Moulin
    Pathologie Aviaire et Parasitologie, INRA, Centre de Tours, Nouzilly, France
    Vet Res 30:299-316. 1999
    ..Hence, vaccination for colibacillosis is not widely practised because of the large variety of serogroups involved in field outbreaks...
  14. ncbi Interdomain interaction in the FimH adhesin of Escherichia coli regulates the affinity to mannose
    Pavel Aprikian
    Department of Microbiology, University of Washington, Seattle, Washington 98105, USA
    J Biol Chem 282:23437-46. 2007
    ..This shift provides a mechanism for FimH-mediated shear-enhanced adhesion by enabling the adhesin to form catch bond-like interactions that are longer lived at high tensile force...
  15. pmc PapD-like chaperones provide the missing information for folding of pilin proteins
    M M Barnhart
    Departments of Molecular Microbiology and Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 97:7709-14. 2000
    ..This mechanism of small periplasmic chaperone function described here deviates from classical hsp60 chaperone-assisted folding...
  16. pmc Catch-bond model derived from allostery explains force-activated bacterial adhesion
    Wendy Thomas
    Department of Bioengineering, University of Washington, Seattle, Washington, USA
    Biophys J 90:753-64. 2006
    ..This strongly suggests that FimH forms allosteric catch bonds. The model advances our understanding of both catch bonds and the role of allostery in regulating protein activity...
  17. ncbi Adhesion properties, fimbrial expression and PCR detection of adhesin-related genes of avian Escherichia coli strains
    Tatiana Amabile de Campos
    Department of Microbiology and Immunology, Biology Institute, CP 6109, Campinas State University Unicamp, Campinas, Sao Paulo, Brazil
    Vet Microbiol 106:275-85. 2005
    ....
  18. ncbi Catch bond-mediated adhesion without a shear threshold: trimannose versus monomannose interactions with the FimH adhesin of Escherichia coli
    Lina M Nilsson
    Departments of Bioengineering and Microbiology, University of Washington, Seattle, WA 98195, USA
    J Biol Chem 281:16656-63. 2006
    ..A shear-dependent conversion of the adhesion mode is also exhibited by P-fimbriated E. coli adhering to digalactose surfaces...
  19. ncbi Receptor binding studies disclose a novel class of high-affinity inhibitors of the Escherichia coli FimH adhesin
    Julie Bouckaert
    Laboratorium voor Ultrastructuur, Vrije Universiteit Brussel and Vlaams Interuniversitair Instituut voor Biotechnologie, Building E, Pleinlaan 2, 1050 Brussels, Belgium
    Mol Microbiol 55:441-55. 2005
    ..coli. We foresee that our findings will spark new ideas and initiatives for the development of UTI vaccines and anti-adhesive drugs to prevent anticipated and recurrent UTIs...
  20. ncbi Analysis of the fim cluster of an avian O2 strain of Escherichia coli: serogroup-specific sites within fimA and nucleotide sequence of fimI
    D Marc
    Institut National de la Recherche Agronomique, Station de Pathologie Aviaire et de Parasitologie, Nouzilly, France
    J Med Microbiol 44:444-52. 1996
    ..In addition, the sequence of the previously uncharacterised gene fimI, which is located between genes fimA and fimC, was determined...
  21. ncbi Shear-dependent 'stick-and-roll' adhesion of type 1 fimbriated Escherichia coli
    Wendy E Thomas
    Department of Bioengineering, University of Washington, Seattle, WA, 98195, USA
    Mol Microbiol 53:1545-57. 2004
    ..This 'stick-and-roll' adhesion could allow type 1 fimbriated bacteria to move along mannosylated surfaces under relatively low flow conditions and to accumulate preferentially in high shear regions...
  22. ncbi Cutting edge: FimH adhesin of type 1 fimbriae is a novel TLR4 ligand
    Karen L Mossman
    Centre for Gene Therapeutics, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada
    J Immunol 181:6702-6. 2008
    ..Our data suggest that TLR4 is a functional receptor for the adhesin portion of bacterial type 1 fimbria...
  23. ncbi Presence in bovine enteropathogenic (EPEC) and enterohaemorrhagic (EHEC) Escherichia coli of genes encoding for putative adhesins of human EHEC strains
    Ioan Mihai Szalo
    Laboratory of Bacteriology, Faculty of Veterinary Medicine, University of Liege, Boulevard de Colonster 20 B43, 4000 Liege, Belgium
    Res Microbiol 153:653-8. 2002
    ..Overall results showed that different genes encoding for putative adhesins of human EHEC strains are present in bovine EPEC and EHEC strains, but not one of them is present in all strains...
  24. ncbi Requirement of Rho-family GTPases in the invasion of Type 1-piliated uropathogenic Escherichia coli
    Juan J Martinez
    Department of Molecular Microbiology and Microbial Pathogenesis, Washington University School of Medicine, St Louis, MO 63110, USA
    Cell Microbiol 4:19-28. 2002
    ..Taken together, these data suggest that FimH-mediated invasion is dependent on GTP-binding protein activity that involves Cdc42 and PI 3-kinase activation probably upstream of Rac1...
  25. ncbi Fimch antiserum inhibits the adherence of Escherichia coli to cells collected by voided urine specimens of diabetic women
    Ruby Meiland
    Department of Medicine, University Medical Center Utrecht, Utrecht, The Netherlands
    J Urol 171:1589-93. 2004
    ..The majority of Escherichia coli strains, which is the most prevalent uropathogen, have type 1 fimbriae that bind to uroplakin in the bladder, as mediated by the adhesin FimH. A vaccine is being developed based on FimH adhesion...
  26. pmc Identification of an aggregative adhesion fimbria (AAF) type III-encoding operon in enteroaggregative Escherichia coli as a sensitive probe for detecting the AAF-encoding operon family
    Christine Bernier
    Unité de Pathogénie Bactérienne des Muqueuses, Groupe d Etude des Infections Diarrhéiques GEID, Institut Pasteur, 75724 Paris Cedex 15, France
    Infect Immun 70:4302-11. 2002
    ..We investigated 32 EAEC isolates, of which only 34.4% were detected with the classical CVD432 probe (detecting pAA virulence plasmids) whereas 65.6% were detected with the AAF probe...
  27. ncbi Resistance of broiler chickens to Escherichia coli respiratory tract infection induced by passively transferred egg-yolk antibodies
    S Kariyawasam
    Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ont, Canada N1G 2W1
    Vet Microbiol 98:273-84. 2004
    ..coli used for challenge. Hence vaccination of broiler breeders to induce anti-PapG and anti-IutA antibodies may provide passive protection of progeny chicks against respiratory/septicemic disease caused by APEC...
  28. ncbi [Candidate vaccine against urinary tract infections]
    I M Hoepelman
    Universitair Medisch Centrum Utrecht UMCU, afd Acute Geneeskunde and Infectieziekten, Postbus 85 500, 3508 GA Utrecht
    Ned Tijdschr Geneeskd 145:1860-2. 2001
    ..In animal studies and in a phase 1 study, this vaccine has proven to be both immunogenic and safe. In this era of increasing resistance to antibiotics, such a method of prevention is of high importance...
  29. ncbi Mannosylated G(0) dendrimers with nanomolar affinities to Escherichia coli FimH
    Mohamed Touaibia
    Equipe PharmaQAM, Department of Chemistry, Universite du Quebec a Montreal, P O Box 8888 Succ Centre Ville Montréal, Quebec, H3C 3P8, Canada
    ChemMedChem 2:1190-201. 2007
    ..Tetramer 4 had a K(d) of 0.45 nM. These clusters were 1000 times more potent than mannose for their capacity to inhibit the binding of E. coli to erythrocytes in vitro...
  30. ncbi Localization of uroplakin Ia, the urothelial receptor for bacterial adhesin FimH, on the six inner domains of the 16 nm urothelial plaque particle
    Guangwei Min
    Structural Biology Program, Skirball Institute of Biomolecular Medicine, New York, NY 10016, USA
    J Mol Biol 317:697-706. 2002
    ....
  31. pmc Novel Zn(2+)-chelating peptides selected from a fimbria-displayed random peptide library
    K Kjaergaard
    Microbial Adhesion Group, Section of Molecular Microbiology, BioCentrum-DTU, Technical University of Denmark, Lyngby
    Appl Environ Microbiol 67:5467-73. 2001
    ..These findings might be applied in the design of biomatrices for bioremediation purposes or in the development of sensors for detection of heavy metals...
  32. pmc Integrin-mediated host cell invasion by type 1-piliated uropathogenic Escherichia coli
    Danelle S Eto
    Division of Cell Biology and Immunology, Pathology Department, University of Utah, Salt Lake City, Utah, United States of America
    PLoS Pathog 3:e100. 2007
    ..Cumulatively, these results indicate that beta1 and alpha3 integrins are functionally important receptors for type 1 pili-expressing bacteria within the urinary tract and possibly at other sites within the host...
  33. pmc The affinity of the FimH fimbrial adhesin is receptor-driven and quasi-independent of Escherichia coli pathotypes
    Julie Bouckaert
    Department of Ultrastructure, Vrije Universiteit Brussel, Flanders Interuniversity Institute for Biotechnology VIB, Pleinlaan 2, 1050 Brussels, Belgium
    Mol Microbiol 61:1556-68. 2006
    ..Our results imply that the carbohydrate expression profile of targeted host tissues and of natural inhibitors in urine, such as Tamm-Horsfall protein, are stronger determinants of adhesion than FimH variation...
  34. pmc Direct evidence that the FimH protein is the mannose-specific adhesin of Escherichia coli type 1 fimbriae
    K A Krogfelt
    Department of Microbiology, Technical University of Denmark, Lyngby
    Infect Immun 58:1995-8. 1990
    ..Furthermore, we show by receptor immunoelectron microscopy that the FimH protein is located laterally in the structure of the type 1 fimbriae...
  35. ncbi Identification and characterization of E. coli type-1 pilus tip adhesion protein
    M S Hanson
    Department of Biological Sciences, University of Pittsburgh, Pennsylvania 15260
    Nature 332:265-8. 1988
    ..This protein is antigenically conserved among strains with different pilin serotypes and is located at the pilus tip...
  36. pmc FimH adhesin of type 1 pili is assembled into a fibrillar tip structure in the Enterobacteriaceae
    C H Jones
    Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110
    Proc Natl Acad Sci U S A 92:2081-5. 1995
    ..The bipartite nature of the type 1 pilus was also demonstrated on pili purified from clinical isolates of members of the Enterobacteriaceae family arguing that it is a conserved feature of the type 1 pilus...
  37. pmc FimH family of type 1 fimbrial adhesins: functional heterogeneity due to minor sequence variations among fimH genes
    E V Sokurenko
    Department of Anatomy, University of Tennessee, Memphis 38163
    J Bacteriol 176:748-55. 1994
    ....
  38. ncbi Distinct glycan structures of uroplakins Ia and Ib: structural basis for the selective binding of FimH adhesin to uroplakin Ia
    Bo Xie
    Mass Spectrometry Resource, Boston University School of Medicine, 670 Albany Street, Boston, MA 02118, USA
    J Biol Chem 281:14644-53. 2006
    ..Results from this study shed light on the molecular pathogenesis of urinary tract infections and may aid in the design of glyco-mimetic inhibitors for preventing and treating this disease...
  39. pmc Weak rolling adhesion enhances bacterial surface colonization
    Brett N Anderson
    Department of Bioengineering, University of Washington, Seattle, WA 98195 5061, USA
    J Bacteriol 189:1794-802. 2007
    ....
  40. ncbi Uroplakin Ia is the urothelial receptor for uropathogenic Escherichia coli: evidence from in vitro FimH binding
    G Zhou
    Skirball Institute of Biomolecular Medicine, Department of Biochemistry, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA
    J Cell Sci 114:4095-103. 2001
    ..coli, and that the binding of uropathogenic bacteria to uroplakin Ia may play a key role in mediating the urothelial responses to bacterial attachment...
  41. pmc Clonal analysis reveals high rate of structural mutations in fimbrial adhesins of extraintestinal pathogenic Escherichia coli
    Scott J Weissman
    Division of Infectious Disease, Immunology and Rheumatology, Department of Pediatrics, University of Washington, Seattle, WA, USA
    Mol Microbiol 59:975-88. 2006
    ..coli are under strong positive selection (Dn/Ds > 1 for fimH and papG) for functionally adaptive amino acid replacements...
  42. ncbi Virulence factors of septicemic Escherichia coli strains
    Daphna Mokady
    Department of Molecular Microbiology and Biotechnology, The George S Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
    Int J Med Microbiol 295:455-62. 2005
    ..The variable profile of virulence genes in septicemic E. coli strains, as well as a prevalence of mobility-related sequences point out the existence of a "mix and match" combinatorial system...
  43. ncbi Molecular epidemiology of extraintestinal pathogenic (uropathogenic) Escherichia coli
    James R Johnson
    Mucosal and Vaccine Research Center, Infectious Diseases 111F, VA Medical Center, 1 Veterans Drive, Minneapolis, MN 55417, USA
    Int J Med Microbiol 295:383-404. 2005
    ..Published by Elsevier GmbH...
  44. ncbi Expression and purification of the mannose recognition domain of the FimH adhesin
    M A Schembri
    Department of Microbiology, Bldg 301, Technical University of Denmark, DK 2800, Lyngby, Denmark
    FEMS Microbiol Lett 188:147-51. 2000
    ..Here we report that a functional form of the FimH receptor-binding domain can be readily isolated and characterised by replacing the C-terminal domain with a histidine tag...
  45. ncbi Roles for Fis and YafK in biofilm formation by enteroaggregative Escherichia coli
    J Sheikh
    Center for Vaccine Development, Department of Pediatrics, University of Maryland School of Medicine, 685 W Baltimore St, Baltimore, MD 21201, USA
    Mol Microbiol 41:983-97. 2001
    ..Our data do not suggest a role for type 1 fimbriae or motility in biofilm formation. EAEC appears to form a novel biofilm, which may be mediated solely by AAF and may reflect its interactions with the intestinal mucosa...
  46. ncbi Bacterial invasion augments epithelial cytokine responses to Escherichia coli through a lipopolysaccharide-dependent mechanism
    J D Schilling
    Department of Molecular Microbiology, Department of Pathology and Immunology, and Department of Internal Medicine, Washington University School of Medicine, St Louis, MO 63110, USA
    J Immunol 166:1148-55. 2001
    ..These results indicate that LPS is required for bacterial invasion to enhance host responses to E. coli within the bladder...
  47. ncbi Aggregative adherence of uropathogenic Proteus mirabilis to cultured epithelial cells
    Sérgio P D Rocha
    Departamento de Microbiologia, Universidade Estadual de Londrina, Parana, Brazil, and Laboratório de Bacteriologia, Instituto Butantan, Sao Paulo, Brazil
    FEMS Immunol Med Microbiol 51:319-26. 2007
    ..The present results reinforce the importance of MR/P as a virulence factor in P. mirabilis due to its association with AA and biofilm formation, which is an important step for the establishment of UTI in catheterized patients...
  48. pmc Uropathogenic Escherichia coli induces extrinsic and intrinsic cascades to initiate urothelial apoptosis
    David J Klumpp
    Department of Urology, Feinberg School of Medicine, Northwestern University, 303 East Chicago Avenue, Chicago, IL 60611, USA
    Infect Immun 74:5106-13. 2006
    ....
  49. pmc P fimbriae, capsule and aerobactin characterize colonic resident Escherichia coli
    F Nowrouzian
    Department of Clinical Immunology, , Sweden
    Epidemiol Infect 126:11-8. 2001
    ..The results indicate that not only P fimbriae, but also, certain capsules and the ability to produce the siderophore aerobactin might contribute to persistence of E. coli in the large intestine...
  50. pmc Maturation of intracellular Escherichia coli communities requires SurA
    Sheryl S Justice
    Department of Pediatrics, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Infect Immun 74:4793-800. 2006
    ..Our data argue that SurA is required within bladder epithelial cells for UPEC to undergo the morphological changes that underlie IBC maturation and completion of the UTI pathogenic cascade...
  51. ncbi FimH-mediated Escherichia coli K1 invasion of human brain microvascular endothelial cells
    Naveed Ahmed Khan
    Division of Pediatric Infectious Diseases, Johns Hopkins University School of Medicine, 600 North Wolfe Street, Park 256, Baltimore, MD 21287, USA
    Cell Microbiol 9:169-78. 2007
    ..Taken together, these findings indicate that FimH induces host cell signalling cascades that are involved in E. coli K1 invasion of HBMEC and CD48 is a putative HBMEC receptor for FimH...
  52. ncbi Intranasal immunization with a recombinant truncated FimH adhesin adjuvanted with CpG oligodeoxynucleotides protects mice against uropathogenic Escherichia coli challenge
    Thelma Verónica Poggio
    Centro Milstein de Ciencia y Tecnología, Centro de Virologia Animal CEVAN, Consejo Nacional de Investigaciones Científicas y Tecnológicas CONICET, Buenos Aires, Argentina
    Can J Microbiol 52:1093-102. 2006
    ..Our results indicate that a recombinant truncated FimH adhesin plus CpG oligodeoxynucleotides is a suitable immunogenic combination that can contribute to the development of a highly efficacious urinary tract infection vaccine...
  53. ncbi Immunization with the binding domain of FimH, the adhesin of type 1 fimbriae, does not protect chickens against avian pathogenic Escherichia coli
    Fréderic Vandemaele
    Laboratory of Physiology and Immunology of Domestic Animals, Faculty of Applied Bioscience and Engineering, Catholic University Leuven, Kasteelpark Arenberg 30, 3001, Leuven, Belgium
    Avian Pathol 34:264-72. 2005
    ..No protective effects of the FimH156 could be detected, confirming the results of the first experiment. Thus, although the FimH156 induced a strong immune response, it was unable to protect chickens against APEC...
  54. pmc Intervening with urinary tract infections using anti-adhesives based on the crystal structure of the FimH-oligomannose-3 complex
    Adinda Wellens
    Department of Molecular and Cellular Interactions, VIB corrected Brussels, Belgium
    PLoS ONE 3:e2040. 2008
    ..Invaded bacteria replicate and mature into dense, biofilm-like inclusions in preparation of fluxing and of infection of neighbouring cells, being the major cause of the troublesome recurrent urinary tract infections...
  55. pmc Adhesins as targets for vaccine development
    T M Wizemann
    MedImmune, Inc, Gaithersburg, Maryland 20878, USA
    Emerg Infect Dis 5:395-403. 1999
    ..With recent advances in the identification, characterization, and isolation of other adhesins, similar approaches are being explored to prevent infections, from otitis media and dental caries to pneumonia and sepsis...
  56. pmc Elevated shear stress protects Escherichia coli cells adhering to surfaces via catch bonds from detachment by soluble inhibitors
    Lina M Nilsson
    Department of Materials, Laboratory for Biologically Oriented Materials, Swiss Federal Institute of Technology, Wolfgang Pauli Strasse 10, ETH Honggerberg, HCI F443, CH 8093 Zurich, Switzerland
    Appl Environ Microbiol 72:3005-10. 2006
    ..Shear stress-enhanced reduction of bacterial detachment has major physiological and therapeutic implications and needs to be considered when developing and screening drugs...
  57. pmc Combined inactivation and expression strategy to study gene function under physiological conditions: application to identification of new Escherichia coli adhesins
    Agnes Roux
    Groupe de Génétique des Biofilms CNRS URA 2172, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France
    J Bacteriol 187:1001-13. 2005
    ..coli to abiotic surfaces. The potential use of the RExBAD approach to study the function of cryptic or uncharacterized genes in large-scale postgenomic functional analyses is discussed...
  58. ncbi Multivariate design, synthesis, and biological evaluation of peptide inhibitors of FimC/FimH protein-protein interactions in uropathogenic Escherichia coli
    Andreas Larsson
    Department of Chemistry, Organic Chemistry, Umea University, SE 901 87 Umea, Sweden
    J Med Chem 48:935-45. 2005
    ..This information can be used in attempts to develop optimized peptides and peptidomimetics that inhibit pilus assembly in pathogenic bacteria...
  59. ncbi Characterization of adhesin variants in Indian isolates of enteroaggregative Escherichia coli
    Renu Bhardwaj
    Department of Experimental Medicine and Biotechnology, Post Graduate Institute of Medical Education and Research, Chandigarh, India
    FEMS Microbiol Lett 258:274-83. 2006
    ..An association between the AAF/adhesin genotypes and the subtypes/scores of phenotypic properties was sought and it was observed that strains harboring same adhesins displayed different subtypes/scores and vice versa...
  60. pmc Virulence properties of Escherichia coli 83972, a prototype strain associated with asymptomatic bacteriuria
    R A Hull
    Department of Microbiology and Immunology, Baylor College of Medicine, University of Texas Health Science Center, Houston, Texas, USA
    Infect Immun 67:429-32. 1999
    ..Limited uroepithelial cell adherence was observed in vivo, and weak D-mannose-sensitive hemagglutination was detected after extended growth in urine in vitro...
  61. ncbi Evidence for linkage between K88ab, K88ac intestinal receptors to Escherichia coli and transferrin loci in pigs
    G Guerin
    Laboratoire de Génétique Biochimique, INRA CRJ, Jouy en Josas, France
    Anim Genet 24:393-6. 1993
    ..16, Zm = 17.9 for the K88acR locus. The estimated recombination rates, smaller in males than in females, are consistent with the order TF-K88abR-K88acR. This linkage thus localizes the K88 loci, as the TF locus, on chromosome 13...
  62. ncbi Distribution of papG alleles among uropathogenic Escherichia coli isolated from different species
    C Feria
    Centro de Investigaçao Interdisciplinar en Sanidade Animal, Faculdade de Medicina Veterinaria, Universidade Tecnica de Lisboa, 1300 477 Lisbon, Portugal
    FEMS Microbiol Lett 202:205-8. 2001
    ..Our findings show that uropathogenic E. coli isolates from different species can have the same papG alleles and thus may have zoonotic potential...
  63. pmc Differential stability and trade-off effects of pathoadaptive mutations in the Escherichia coli FimH adhesin
    Scott J Weissman
    Department of Microbiology, University of Washington School of Medicine, 1959 North Pacific Street, Seattle, WA 98195, USA
    Infect Immun 75:3548-55. 2007
    ..These functional trade-offs may determine the natural populational instability of this mutation or other pathoadaptive FimH mutations that confer dramatic increases in 1M binding strength...
  64. ncbi Virulence characteristics and phylogenetic background of multidrug-resistant and antimicrobial-susceptible clinical isolates of Escherichia coli from across the United States, 2000-2001
    James R Johnson
    Mucosal and Vaccine Research Center, Veterans Affairs Medical Center, and Department of Medicine, University of Minnesota, Minneapolis, Minnesota 55417, USA
    J Infect Dis 190:1739-44. 2004
    ..Increases in antimicrobial resistance in Escherichia coli have been paralleled by an increasing incidence of E. coli sepsis, suggesting a possible link between resistance and virulence...
  65. pmc Biofilm formation in a hydrodynamic environment by novel fimh variants and ramifications for virulence
    M A Schembri
    Department of Microbiology, Technical University of Denmark, DK-2800 Lyngby, Denmark
    Infect Immun 69:1322-8. 2001
    ..The study indicates that UTI FimH variants possess adaptations that enhance biofilm formation and suggests a novel role for FimH in UTIs associated with medical implants such as catheters...
  66. pmc Predominance of afr2 and ral fimbrial genes related to those encoding the K88 and CS31A fimbrial adhesins in enteropathogenic Escherichia coli isolates from rabbits with postweaning diarrhea in Central Europe
    Mohamed A Dow
    Veterinary Medical Research Institute of the Hungarian Academy of Sciences, 1143 Budapest, Hungaria Str 21, Budapest, Hungary
    J Clin Microbiol 43:1366-71. 2005
    ..Several EPEC strains (i.e., O153:H7 and O157:H2) lacked these genes but did adhere to HeLa cells and produced attaching and effacing lesions in rabbits...
  67. pmc Identification and characterization of the locus for diffuse adherence, which encodes a novel afimbrial adhesin found in atypical enteropathogenic Escherichia coli
    Isabel C A Scaletsky
    Department of Microbiology and Immunology, Universidade Federal de Sao Paulo, Sao Paulo, Brazil 04023 062
    Infect Immun 73:4753-65. 2005
    ..coli DH5alpha lda(+) clone to HEp-2 cells. Electron microscopy revealed a nonfimbrial structure surrounding the bacterial cell...
  68. ncbi Basis for N-acetyllactosamine-mediated inhibition of enteropathogenic Escherichia coli localized adherence
    Romney M Hyland
    Department of Microbiology and Infectious Diseases, Faculty of Medicine, University of Calgary, 3330 Hospital Drive, NW, Calgary, AB, T2N 4N1, Canada
    J Med Microbiol 55:669-75. 2006
    ..This could then expedite the intimate adherence phase of the multi-step EPEC colonization process by drawing the organisms closer to the host-cell plasma membrane...
  69. ncbi Prevalence of fimbial colonization factors F18ab and F18ac in Escherichia coli isolates from weaned piglets with edema and/or diarrhea in China
    Darong Cheng
    College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China
    Vet Microbiol 110:35-9. 2005
    ..These results suggest that the duplex PCR performed in this experiment is a more reliable method for identification of F18+E. coli, and that F18 is a more important virulence factor of VTEC and VTEC/ETEC...
  70. pmc Ramifications of kinetic partitioning on usher-mediated pilus biogenesis
    E T Saulino
    Department of Molecular Microbiology and Microbial Pathogenesis, Washington University School of Medicine, 660 South Euclid Avenue, Campus Box 8230, St Louis, MO 63110, USA
    EMBO J 17:2177-85. 1998
    ..The data support a model in which binding of the chaperone-adhesin complex to the usher stabilizes the usher in an assembly-competent conformation and allows initiation of pilus assembly...
  71. ncbi Uropathogenic virulence factors in isolates of Escherichia coli from clinical cases of canine pyometra and feces of healthy bitches
    Yvette M M Chen
    Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee 3030, Vic, Australia
    Vet Microbiol 94:57-69. 2003
    ....
  72. ncbi Histone H1 proteins act as receptors for the 987P fimbriae of enterotoxigenic Escherichia coli
    Guoqiang Zhu
    Department of Pathobiology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, 19104, USA
    J Biol Chem 280:23057-65. 2005
    ..There, the histone H1 molecules stabilize the sulfatide-fimbriae interaction by simultaneously binding to the membrane and to 987P...
  73. pmc Pathogenesis of Afa/Dr diffusely adhering Escherichia coli
    Alain L Servin
    Institut National de la Sante et de la Recherche Medicale, Unité 510, Faculte de Pharmacie Paris XI, Chatenay Malabry, France
    Clin Microbiol Rev 18:264-92. 2005
    ..Finally, the presence of other, unknown virulence factors and the way that an Afa/Dr DAEC strain emerges from the human intestinal microbiota as a "silent pathogen" are discussed...
  74. pmc Canine feces as a reservoir of extraintestinal pathogenic Escherichia coli
    J R Johnson
    VA Medical Center and Department of Medicine, University of Minnesota, Minneapolis, Minnesota 55417, USA
    Infect Immun 69:1306-14. 2001
    ..These data confirm that ExPEC representing known virulent clones are highly prevalent in canine feces, which consequently may provide a reservoir of ExPEC for acquisition by humans...
  75. ncbi Characterization of fimbriae extracts from porcine enterotoxigenic Escherichia coli strains carrying F6 (987P) antigen
    Froilán Vázquez
    E coli Reference Laboratory, Faculty of Veterinary, University of Santiago de Compostela, Lugo, Spain
    Int Microbiol 9:241-6. 2006
    ..5 and 21.9 kDa were detected. The 21.9-kDa band was identified as the major subunit of F6 fimbrial antigen in strains of serogroups O9 and O141. The 17.5-kDa band was associated with porcine strains of serogroups O9 and O20...
  76. ncbi PapG-dependent adherence breaks mucosal inertia and triggers the innate host response
    Goran Bergsten
    Department of Microbiology, Institute of Laboratory Medicine, Lund University, Lund, Sweden
    J Infect Dis 189:1734-42. 2004
    ..We conclude that PapG-mediated adherence breaks mucosal inertia in the human urinary tract by triggering innate immunity and propose that this activation step differentiates asymptomatic carriage from infection...
  77. ncbi FimH-mediated autoaggregation of Escherichia coli
    M A Schembri
    Microbial Adhesion Group, Section of Molecular Microbiology, BioCentrum DTU, Bldg 301, Technical University of Denmark, DK 2800 Lyngby, Denmark
    Mol Microbiol 41:1419-30. 2001
    ..We present evidence that autoaggregating versions of FimH also occur in nature. Our results stress the highly adaptive nature of the ubiquitous FimH adhesin...
  78. ncbi Genetically engineered enteropathogenic Escherichia coli strain elicits a specific immune response and protects against a virulent challenge
    Severine Boullier
    UMR 1225, Institut National de la Recherche Agronomique, Ecole Nationale Veterinaire de Toulouse, 31076 Toulouse Cedex 3, France
    Microbes Infect 5:857-67. 2003
    ..These Abs prevent bacterial attachment to epithelial cells in vitro. These results open the way for the development of an efficient vaccine strategy against rabbit EPEC infections...
  79. pmc Phylogenetic analysis and prevalence of urosepsis strains of Escherichia coli bearing pathogenicity island-like domains
    Martine Bingen-Bidois
    Laboratoire de Bacteriologie, Centre Hospitalier de Gonesse, Gonesse Service de Microbiologie, Hopital Robert Debre, Paris, France
    Infect Immun 70:3216-26. 2002
    ..This study provides new data on the prevalence and variability of physical genetic linkage between pap and certain virulence-associated genes that are consistent with their colocalization on archetypal PAIs...
  80. ncbi The fimbrial adhesin F17-G of enterotoxigenic Escherichia coli has an immunoglobulin-like lectin domain that binds N-acetylglucosamine
    Lieven Buts
    Department of Ultrastructure, Institute for Molecular Biology, Vrije Universiteit Brussel, Vlaams Interuniversitair Instituut voor Biotechnologie, Brussels, Belgium
    Mol Microbiol 49:705-15. 2003
    ....
  81. ncbi Characterization of a plasmid-encoded adhesin of an avian pathogenic Escherichia coli (APEC) strain isolated from a case of swollen head syndrome (SHS)
    Eliana Guedes Stehling
    Department of Microbiology and Immunology, Biology Institute, CP 6109, Campinas State University, Campinas, SP, Brazil
    Vet Microbiol 95:111-20. 2003
    ..Based on these results, we suggest that the 60MDa plasmid have adhesion genes, which may be responsible for the initial colonization of the upper respiratory tract of chickens...
  82. ncbi The Pix pilus adhesin of the uropathogenic Escherichia coli strain X2194 (O2 : K(-): H6) is related to Pap pili but exhibits a truncated regulatory region
    Andreas Lugering
    Institut für Infektiologie, Zentrum fur Molekularbiologie der Entzundung, Von Esmarch Strasse 56, Westfalische Wilhelms Universitat Munster, Germany
    Microbiology 149:1387-97. 2003
    ..In contrast to other filamentous adhesin systems, Pix pili are mainly expressed in the steady state growth phase and were not repressed by the addition of glucose...
  83. ncbi A plasmid-encoded type IV fimbrial gene of enteropathogenic Escherichia coli associated with localized adherence
    M S Donnenberg
    Department of Veterans Affairs Medical Center, Baltimore, Maryland 21201
    Mol Microbiol 6:3427-37. 1992
    ..These studies describe an EPEC plasmid-encoded fimbrial gene, a candidate for the elusive EPEC adherence factor responsible for localized adherence...
  84. pmc Uncoiling mechanics of Escherichia coli type I fimbriae are optimized for catch bonds
    Manu Forero
    Department of Materials, Laboratory for Biologically Oriented Materials, ETH Zurich, Zurich, Switzerland
    PLoS Biol 4:e298. 2006
    ..This suggests that the mechanical properties of the fimbrial shaft have codeveloped to optimize the stability of the terminal adhesive under flow...
  85. pmc Role of type 1 fimbria- and P fimbria-specific adherence in colonization of the neurogenic human bladder by Escherichia coli
    Richard A Hull
    Department of Molecular Virology and Microbiology, Center for Prostheses Infections, Baylor College of Medicine, Houston, Texas 77030, USA
    Infect Immun 70:6481-4. 2002
    ..The implications of these results with regard to the selection of potential vaccine antigens for the prevention of urinary tract infection are discussed...
  86. ncbi Type 1, P and S fimbriae, and afimbrial adhesin I are not essential for uropathogenic Escherichia coli to adhere to and invade bladder epithelial cells
    Jun Miyazaki
    Department of Infection Biology, Institute of Basic Medical Sciences, University of Tsukuba, 1 1 1 Tennodai, Tsukuba shi, Ibaraki 305 8575, Japan
    FEMS Immunol Med Microbiol 33:23-6. 2002
    ..In conclusion, this study demonstrates that type 1 fimbriae, P fimbriae, S fimbriae, and afimbrial adhesin I are not required for UPEC to adhere to and invade uroepithelial cells...
  87. ncbi Role of type I fimbriae in the aggregative adhesion pattern of enteroaggregative Escherichia coli
    Cristiano G Moreira
    Laboratório Especial de Microbiologia, Instituto Butantan, Avenida Vital Brazil 1500, 05503 900, SP, Sao Paulo, Brazil
    FEMS Microbiol Lett 226:79-85. 2003
    ..Our data evidence a role for TIF in the AA pattern and in EAEC biofilm formation, demonstrating that these phenotypes are multifactorial...
  88. ncbi The inner membrane subassembly of the enteropathogenic Escherichia coli bundle-forming pilus machine
    Lynette J Crowther
    Division of Infectious Diseases, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Mol Microbiol 52:67-79. 2004
    ..Furthermore, we found that the putative ATPases BfpD and BfpF play antagonistic roles in BFP biogenesis and retraction, respectively, by interacting with distinct domains of the BFP biogenesis machine...
  89. ncbi Decreased predominance of papG class II allele in Escherichia coli strains isolated from adults with acute pyelonephritis and urinary tract abnormalities
    C C Tseng
    Departments of Internal Medicine, Pathology and Medical Technology and Institute of Clinical Medicine, National Cheng Kung University, Medical College, Tainan, Taiwan, Republic of China
    J Urol 166:1643-6. 2001
    ..coli infection in patients with acute pyelonephritis and a normal urinary tract, while urinary tract abnormalities and/or obstruction may permit ascending infection of E. coli strains with lower adhesive ability...
  90. ncbi [Molecular analysis of uropathogenic strains of Escherichia coli and the clinical course of urinary tract infection in children]
    Marlena Rytlewska
    Klinika Pediatrii, Gastroenterologii i Onkologii Dzieciecej, Akademia Medyczna, ul Nowe Ogrody 1 6, 80 803 Gdansk, Poland
    Med Wieku Rozwoj 9:727-39. 2005
    ..Determination of bacterial virulence factors causing urinary tract infections in children may be helpful in the prognosis of the course of the disease...
  91. ncbi Roles of host and bacterial virulence factors in the development of upper urinary tract infection caused by Escherichia coli
    Chin Chung Tseng
    Division of Nephrology, Department of Internal Medicine, National Cheng Kung University Hospital, Tainan, Taiwan, Republic of China
    Am J Kidney Dis 39:744-52. 2002
    ..In conclusion, both host and E coli virulence factors contribute to the development of upper UTI, and less virulent strains can cause upper UTI in hosts with predisposing factors...
  92. ncbi Predominance of the papGII allele with high sequence homology to that of human isolates among avian pathogenic Escherichia coli (APEC)
    Fréderic J Vandemaele
    Laboratory of Physiology and Immunology of Domestic Animals, Catholic University Leuven, Kasteelpark Arenberg 30, 3001 Leuven, Belgium
    Vet Microbiol 97:245-57. 2003
    ..Finally, the papGII and papGIII sequences showed high homology with mammalian (including human) source papG sequences...
  93. pmc Purification of the Escherichia coli type 1 pilin and minor pilus proteins and partial characterization of the adhesin protein
    M S Hanson
    Department of Biological Sciences, University of Pittsburgh, Pennsylvania 15260
    J Bacteriol 170:3350-8. 1988
    ..5K and 14.5K proteins. We have determined the amino acid composition and amino-terminal sequence of the adhesion protein. This sequence shows limited homology with the amino-terminal sequences of several E. coli pilins, including type 1...
  94. ncbi Asymptomatic bacteriuria Escherichia coli strains: adhesins, growth and competition
    Viktoria Roos
    Microbial Adhesion Group, Center for Biomedical Microbiology, BioCentrum DTU, Technical University of Denmark, Lyngby, Denmark
    FEMS Microbiol Lett 262:22-30. 2006
    ..Our results support the notion that for strains with reduced capacity to express fimbriae, the ability to grow fast in human urine becomes crucial for colonization of the urinary tract...
  95. doi The Yersinia pestis autotransporter YapC mediates host cell binding, autoaggregation and biofilm formation
    Suleyman Felek
    Department of Biologic and Materials Sciences, University of Michigan School of Dentistry, Ann Arbor, MI 48109 1078, USA
    Microbiology 154:1802-12. 2008
    ..These data demonstrate several activities for YapC that may function during Y. pestis infection...
  96. pmc Escherichia coli DraE adhesin-associated bacterial internalization by epithelial cells is promoted independently by decay-accelerating factor and carcinoembryonic antigen-related cell adhesion molecule binding and does not require the DraD invasin
    Natalia Korotkova
    Department of Microbiology, University of Washington, Seattle, Washington 98195 7242, USA
    Infect Immun 76:3869-80. 2008
    The Dr family of Escherichia coli adhesins are virulence factors associated with diarrhea and urinary tract infections. Dr fimbriae are comprised of two subunits...
  97. pmc Afa/Dr-expressing, diffusely adhering Escherichia coli strain C1845 triggers F1845 fimbria-dependent phosphatidylserine externalization on neutrophil-like differentiated PLB-985 cells through an apoptosis-independent mechanism
    Nicolas Sémiramoth
    INSERM, UMR756 Signalisation et Physiopathologie des Cellules Epithéliales, and Universite Paris Sud XI, Faculte de Pharmacie, Chatenay Malabry, France
    Infect Immun 78:2974-83. 2010
    ..Interestingly, although apoptosis pathways were not engaged, C1845-infected PLB-985 cells displayed enhanced removal by macrophages, a process that may participate in the resolution of Afa/Dr DAEC infection and related inflammation...
  98. ncbi The long-term cytoskeletal rearrangement induced by rabbit enteropathogenic Escherichia coli is Esp dependent but intimin independent
    J P Nougayrede
    Unité Associée Microbiologie Moléculaire, Institut National de la Recherche Agronomique Ecole Nationale Vétérinaire de Toulouse, France
    Mol Microbiol 31:19-30. 1999
    ..In conclusion, the CPE is the first example of an Esp-dependent but Eae (intimin)-independent alteration of the host cell cytoskeleton by certain EPEC strains...
  99. pmc The solution structure of the invasive tip complex from Afa/Dr fibrils
    Ernesto Cota
    Division of Molecular Biosciences, Biochemistry Building, Imperial College London, South Kensington, London SW7 2AZ, UK
    Mol Microbiol 62:356-66. 2006
    ....
  100. ncbi Impairments in enzyme activity and biosynthesis of brush border-associated hydrolases in human intestinal Caco-2/TC7 cells infected by members of the Afa/Dr family of diffusely adhering Escherichia coli
    I Peiffer
    , , , , France
    Cell Microbiol 3:341-57. 2001
    ..Moreover, the results revealed that other pathogenic factor(s) distinct from the Afa/Dr adhesins may play(s) a crucial role in this mechanism of pathogenicity...
  101. ncbi Differential recognition of members of the carcinoembryonic antigen family by Afa/Dr adhesins of diffusely adhering Escherichia coli (Afa/Dr DAEC)
    Cedric N Berger
    Institut National de la Sante et de la Recherche Medicale INSERM, Unité 510, Faculte de Pharmacie Paris XI, F 92296 Chatenay Malabry, France
    Mol Microbiol 52:963-83. 2004
    ..Moreover, recognition of CEA is accompanied by an activation of the Rho GTPase Cdc42 and by a phosphorylation of ERM, which in turn elicit the observed cell surface microvilli-like extensions...

Research Grants72

  1. CD556, Infection, and Race in Preterm Delivery
    Stella Nowicki; Fiscal Year: 2005
    ..The long-term goal of our project is to characterize mechanism of infection associated PTL/PTD and the role of the host and pathogen factors interacting at the urogenital interphase in birth outcome...
  2. CD556, Infection, and Race in Preterm Delivery
    Bogdan Nowicki; Fiscal Year: 2004
    ..The long-term goal of our project is to characterize mechanism of infection associated PTL/PTD and the role of the host and pathogen factors interacting at the urogenital interphase in birth outcome...
  3. Dynamic Properties of Bacterial Adhesions
    Evgeni Sokurenko; Fiscal Year: 2005
    ..abstract_text> ..
  4. Pathoadaptive evolution of Salmonella
    Evgeni Sokurenko; Fiscal Year: 2009
    ..We will determine genetic changes that contribute to emergence of Salmonella that are infecting warm-blooded animals and cause typhoid or non-typhoid disease in humans. ..
  5. Infectious Disease Training in Clinical Investigation
    James Johnson; Fiscal Year: 2007
    ....
  6. PATHOGENIC ADAPTATION OF MICROBIAL ADHESINS
    Evgeni Sokurenko; Fiscal Year: 2009
    ..Studies proposed here will serve as model ones to provide a spectrum of novel information regarding shear effects on bacterial adhesion, particularlyin connection to the bacterial pathogenesis. ..
  7. Dynamic Properties of Bacterial Adhesins
    Evgeni Sokurenko; Fiscal Year: 2007
    ....
  8. Resistant E.coli in Humans and Poultry
    James Johnson; Fiscal Year: 2005
    ..Does the research address a current and compelling problem of antimicrobial resistance that is of high public health importance and for which research is needed? [yes]. ..
  9. Pathoadaptive evolution of Salmonella
    EVGENI VENIAMINOVIC contact SOKURENKO; Fiscal Year: 2010
    ..We will determine genetic changes that contribute to emergence of Salmonella that are infecting warm-blooded animals and cause typhoid or non-typhoid disease in humans. ..
  10. PATHOGENIC ADAPTATION OF MICROBIAL ADHESINS
    Evgeni Sokurenko; Fiscal Year: 2007
    ..Studies proposed here will serve as model ones to provide a spectrum of novel information regarding shear effects on bacterial adhesion, particularly in connection to the bacterial pathogenesis. ..
  11. Dynamic Properties of Bacterial Adhesins
    EVGENI VENIAMINOVIC SOKURENKO; Fiscal Year: 2010
    ....
  12. PATHOGENIC ADAPTATION OF MICROBIAL ADHESINS
    EVGENI VENIAMINOVIC SOKURENKO; Fiscal Year: 2010
    ..Studies proposed here will serve as model ones to provide a spectrum of novel information regarding shear effects on bacterial adhesion, particularlyin connection to the bacterial pathogenesis. ..
  13. INTERLEUKIN-12 REGULATION BY DR-FIMBRIATED E. COLI
    Pawel Goluszko; Fiscal Year: 2002
    ..The investigators propose to evaluate in three Specific Aims the general hypothesis that cross-linking of DAF on human monocytes/macrophages by E. coli bearing Dr fimbriae inhibits IL-2 production. ..
  14. PATHOGENIC ADAPTATION OF MICROBIAL ADHESINS
    Evgeni Sokurenko; Fiscal Year: 2004
    ..Such information may be useful in optimizing methods to prevent dissemination of pathogens from the human gut. ..
  15. Long Polar Fimbriae of Attaching and Effacing Escherichia coli
    Alfredo Torres; Fiscal Year: 2009
    ..new and deeper insights into the process of AEEC colonization, and a better understanding of their regulatory mechanisms controlling expression, which will be a basis for developing novel therapeutics and simplified diagnostic tests ..
  16. Bacterial interactions in biofilms
    Richard Hull; Fiscal Year: 2004
    ..Results of the proposed experiments may illuminate new targets for intervention therapies that disrupt biofilm formation. This may lead to more effective treatment of catheter-associated infections. ..
  17. Long Polar Fimbriae of Attaching and Effacing Escherichia coli
    Alfredo Torres; Fiscal Year: 2009
    ..new and deeper insights into the process of AEEC colonization, and a better understanding of their regulatory mechanisms controlling expression, which will be a basis for developing novel therapeutics and simplified diagnostic tests ..
  18. Long Polar Fimbriae of Attaching and Effacing Escherichia coli
    Alfredo G Torres; Fiscal Year: 2010
    ..new and deeper insights into the process of AEEC colonization, and a better understanding of their regulatory mechanisms controlling expression, which will be a basis for developing novel therapeutics and simplified diagnostic tests ..
  19. ROLE OF TNF IN BLADDER INFLAMMATION
    David J Klumpp; Fiscal Year: 2010
    ..This project will determine the prevalence of RANTES as a novel IC biomarker in urines and then quantify the efficacy of mechanistically-directed therapies in murine models. ..
  20. Mucosal Therapy for Autoimmunity
    David W Pascual; Fiscal Year: 2010
    ..Thus, once we are able to establish the ps1 delivery technology, we will move forward to use current models for Sj"gren's Syndrome to test if disease progression can be reduced. ..
  21. MUCOSAL IMMUNITY ELICITED BY SALMONELLA VACCINE DELIVERY
    David Pascual; Fiscal Year: 2009
    ..Thus, from these studies we will learn the specific mechanisms induced by this anti-inflammatory vaccine. ..
  22. MOLECULAR ANALYSIS OF MICROBIAL PATHOGENS
    RALPH ISBERG; Fiscal Year: 2007
    ..All faculty members of the Program have individual NIH grants or other forms of support. The application is for 5 years, with 5 predoctoral trainees requested for each of years 01 -05. ..
  23. MAST CELLS IN INNATE IMMUNITY AGAINST BACTERIAL INFECTIO
    Ravi Malaviya; Fiscal Year: 2001
    ..These studies will provide new insights regarding the participation of mast cells in innate host defense against pathogenic gram negative bacteria. ..
  24. TARGETED DNA DELIVERY FOR SALIVARY IGA RESPONSES
    David Pascual; Fiscal Year: 2004
    ..These studies will provide the basis for future development of subunit vaccines to target the NALT for eliciting protective immunity in the oral cavity and SALT. ..
  25. MUCOSAL IMMUNITY ELICITED BY SALMONELLA VACCINE DELIVERY
    David Pascual; Fiscal Year: 2001
    ..abstract_text> ..
  26. MOLECULAR BASIS OF YERSINIA/HOST CELL INTERACTION
    RALPH ISBERG; Fiscal Year: 2007
    ..In addition, identification of the components that allow a simple organism to enter an animal cell could result in new techniques to introduce therapeutic agents that would otherwise not be able to enter the host cell...
  27. MUCOSAL IMMUNITY ELICITED BY SALMONELLA VACCINE DELIVERY
    David Pascual; Fiscal Year: 2007
    ..Thus, from these studies we will learn the specific mechanisms induced by this anti-inflammatory vaccine. ..
  28. ROLE OF BLADDER AND VAGINAL EPITHELIAL CAVEOLAE IN UTIs
    Ann Stapleton; Fiscal Year: 2006
    ..These studies will advance knowledge of the role of GSLs and caveolae, fundamental cellular components, in the pathogenesis of E. coli UTI. ..
  29. ROLE OF BLADDER AND VAGINAL EPITHELIAL CAVEOLAE IN UTIs
    Ann Stapleton; Fiscal Year: 2007
    ..These studies will advance knowledge of the role of GSLs and caveolae, fundamental cellular components, in the pathogenesis ofE. coli UTI. ..
  30. ROLE OF TNF IN BLADDER INFLAMMATION
    David Klumpp; Fiscal Year: 2005
    ..Thus, this project will determine the role of TNF in bladder inflammation and examine a potential therapy for IC. ..
  31. Mucosal Lymphocyte Homing in the Head and Neck
    David Pascual; Fiscal Year: 2007
    ..n. challenge with CT. From this work, we will learn how routes of immunizations can be used to effectively target vaccines to nonintestinal mucosal sites. ..