viral core proteins

Summary

Summary: Proteins found mainly in icosahedral DNA and RNA viruses. They consist of proteins directly associated with the nucleic acid inside the NUCLEOCAPSID.

Top Publications

  1. ncbi The lipid droplet is an important organelle for hepatitis C virus production
    Yusuke Miyanari
    Department of Viral Oncology, Institute for Virus Research, Kyoto University, Kyoto 606 8507, Japan
    Nat Cell Biol 9:1089-97. 2007
  2. pmc Preliminary assessment of the efficacy of a T-cell-based influenza vaccine, MVA-NP+M1, in humans
    Patrick J Lillie
    Jenner Institute, University of Oxford, UK
    Clin Infect Dis 55:19-25. 2012
  3. pmc Potent CD8+ T-cell immunogenicity in humans of a novel heterosubtypic influenza A vaccine, MVA-NP+M1
    Tamara K Berthoud
    The Jenner Institute, Oxford University, Oxford, United Kingdom
    Clin Infect Dis 52:1-7. 2011
  4. ncbi The influenza virus nucleoprotein: a multifunctional RNA-binding protein pivotal to virus replication
    Agustín Portela
    División de Productos Biológicos y Biotecnología, Agencia Española del Medicamento, Majadahonda 28220, Madrid, Spain
    J Gen Virol 83:723-34. 2002
  5. pmc Contributions of antinucleoprotein IgG to heterosubtypic immunity against influenza virus
    Mark W Lamere
    Division of Allergy, Immunology, and Rheumatology, Department of Medicine, University of Rochester Medical Center, Rochester, NY 14642, USA
    J Immunol 186:4331-9. 2011
  6. ncbi The mechanism by which influenza A virus nucleoprotein forms oligomers and binds RNA
    Qiaozhen Ye
    Department of Biochemistry and Cell Biology, Rice University, 6100 Main Street, MS140, Houston, Texas 77005, USA
    Nature 444:1078-82. 2006
  7. pmc Consensus siRNA for inhibition of HCV genotype-4 replication
    Abdel Rahman N Zekri
    Virology and Immunology Unit, Cancer Biology Department, National Cancer Institute, Cairo University, 1st Kasr El Aini st, Cairo, Egypt
    Virol J 6:13. 2009
  8. pmc Formation of a human immunodeficiency virus type 1 core of optimal stability is crucial for viral replication
    Brett M Forshey
    Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Virol 76:5667-77. 2002
  9. ncbi Association of amino acid substitution pattern in core protein of hepatitis C virus genotype 1b high viral load and non-virological response to interferon-ribavirin combination therapy
    Norio Akuta
    Department of Gastroenterology, Toranomon Hospital, Tokyo, Japan
    Intervirology 48:372-80. 2005
  10. pmc Interaction of the influenza virus nucleoprotein with the cellular CRM1-mediated nuclear export pathway
    D Elton
    Division of Virology, Department of Pathology, University of Cambridge, Cambridge CB2 1QP, United Kingdom
    J Virol 75:408-19. 2001

Detail Information

Publications345 found, 100 shown here

  1. ncbi The lipid droplet is an important organelle for hepatitis C virus production
    Yusuke Miyanari
    Department of Viral Oncology, Institute for Virus Research, Kyoto University, Kyoto 606 8507, Japan
    Nat Cell Biol 9:1089-97. 2007
    ..This study reveals a novel function of LDs in the assembly of infectious HCV and provides a new perspective on how viruses usurp cellular functions...
  2. pmc Preliminary assessment of the efficacy of a T-cell-based influenza vaccine, MVA-NP+M1, in humans
    Patrick J Lillie
    Jenner Institute, University of Oxford, UK
    Clin Infect Dis 55:19-25. 2012
    ..Following a phase 1 clinical study that demonstrated vaccine safety and immunogenicity, a phase 2a vaccination and influenza challenge study has been conducted in healthy adult volunteers...
  3. pmc Potent CD8+ T-cell immunogenicity in humans of a novel heterosubtypic influenza A vaccine, MVA-NP+M1
    Tamara K Berthoud
    The Jenner Institute, Oxford University, Oxford, United Kingdom
    Clin Infect Dis 52:1-7. 2011
    ..A vaccine providing protective immunity to the highly conserved internal antigens could provide longer-lasting protection against multiple influenza subtypes...
  4. ncbi The influenza virus nucleoprotein: a multifunctional RNA-binding protein pivotal to virus replication
    Agustín Portela
    División de Productos Biológicos y Biotecnología, Agencia Española del Medicamento, Majadahonda 28220, Madrid, Spain
    J Gen Virol 83:723-34. 2002
    ..The evidence for the existence of each of these activities and their possible roles in transcription, replication and intracellular trafficking of the virus genome is considered...
  5. pmc Contributions of antinucleoprotein IgG to heterosubtypic immunity against influenza virus
    Mark W Lamere
    Division of Allergy, Immunology, and Rheumatology, Department of Medicine, University of Rochester Medical Center, Rochester, NY 14642, USA
    J Immunol 186:4331-9. 2011
    ..The high conservation of NP Ag and the known longevity of Ab responses suggest that anti-NP IgG may provide a critically needed component of a universal influenza vaccine...
  6. ncbi The mechanism by which influenza A virus nucleoprotein forms oligomers and binds RNA
    Qiaozhen Ye
    Department of Biochemistry and Cell Biology, Rice University, 6100 Main Street, MS140, Houston, Texas 77005, USA
    Nature 444:1078-82. 2006
    ..Our results identify the tail loop binding pocket as a potential target for antiviral development...
  7. pmc Consensus siRNA for inhibition of HCV genotype-4 replication
    Abdel Rahman N Zekri
    Virology and Immunology Unit, Cancer Biology Department, National Cancer Institute, Cairo University, 1st Kasr El Aini st, Cairo, Egypt
    Virol J 6:13. 2009
    ..The current study was conducted to assess whether siRNA can inhibit different HCV genotypes with many quasispecies and to assess whether consensus siRNA have the same effect as regular siRNA...
  8. pmc Formation of a human immunodeficiency virus type 1 core of optimal stability is crucial for viral replication
    Brett M Forshey
    Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Virol 76:5667-77. 2002
    ....
  9. ncbi Association of amino acid substitution pattern in core protein of hepatitis C virus genotype 1b high viral load and non-virological response to interferon-ribavirin combination therapy
    Norio Akuta
    Department of Gastroenterology, Toranomon Hospital, Tokyo, Japan
    Intervirology 48:372-80. 2005
    ..Non-virological responders (NVRs, namely ultimate resistant cases) who do not achieve HCV-RNA negativity during treatment are also encountered. We investigated the pretreatment virological features of NVRs...
  10. pmc Interaction of the influenza virus nucleoprotein with the cellular CRM1-mediated nuclear export pathway
    D Elton
    Division of Virology, Department of Pathology, University of Cambridge, Cambridge CB2 1QP, United Kingdom
    J Virol 75:408-19. 2001
    ..Consistent with this, NP bound CRM1 in vitro. Overall, these data raise the possibility that RNP export is mediated by a direct interaction between NP and the cellular CRM1 export pathway...
  11. ncbi Robust hepatitis C genotype 3a cell culture releasing adapted intergenotypic 3a/2a (S52/JFH1) viruses
    Judith M Gottwein
    Department of Infectious Diseases and Clinical Research Center, Hvidovre, Denmark
    Gastroenterology 133:1614-26. 2007
    ..We aimed at exploiting the unique JFH1 replication characteristics to develop culture systems for genotype 3a, which has a high prevalence worldwide...
  12. ncbi Hepatitis C virus core protein induces lipid droplet redistribution in a microtubule- and dynein-dependent manner
    Steeve Boulant
    MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK
    Traffic 9:1268-82. 2008
    ..We propose that the presence of core on LDs favours their movement towards the nucleus, possibly to increase the probability of interaction between sites of HCV RNA replication and virion assembly...
  13. ncbi Hepatitis C virus core protein inhibits microsomal triglyceride transfer protein activity and very low density lipoprotein secretion: a model of viral-related steatosis
    Gabriel Perlemuter
    Liver Cancer and Molecular Virology, Institut National de la Santé et de la Recherche Médicale Unité 370, Faculte de Medecine Necker Enfants Malades, 75730 Paris Cedex 15, France
    FASEB J 16:185-94. 2002
    ....
  14. ncbi Regulation of hepatitis C virus secretion by the Hrs-dependent exosomal pathway
    Keiichi Tamai
    Division of Cancer Biology and Therapeutics, Miyagi Cancer Center Research Institute, Natori 981 1293, Japan
    Virology 422:377-85. 2012
    ..These results suggested that HCV secretion from host cells requires Hrs-dependent exosomal pathway in which the viral assembly is also involved...
  15. pmc A novel role for non-neutralizing antibodies against nucleoprotein in facilitating resistance to influenza virus
    Damian M Carragher
    Trudeau Institute, Saranac Lake, NY 12983, USA
    J Immunol 181:4168-76. 2008
    ..These results suggest that vaccines designed to elicit optimal heterosubtypic immunity to influenza should promote both Ab and T cell responses to conserved internal proteins...
  16. pmc Oligomerization of Ebola virus VP40 is essential for particle morphogenesis and regulation of viral transcription
    T Hoenen
    Institut fur Virologie, Hans Meerwein Str 2, 35043 Marburg, Germany
    J Virol 84:7053-63. 2010
    ....
  17. pmc Model suggesting that replication of influenza virus is regulated by stabilization of replicative intermediates
    Frank T Vreede
    Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom
    J Virol 78:9568-72. 2004
    ....
  18. pmc Trans-complementation of an NS2 defect in a late step in hepatitis C virus (HCV) particle assembly and maturation
    Minkyung Yi
    Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, TX, USA
    PLoS Pathog 5:e1000403. 2009
    ..In aggregate, these results indicate that NS2 functions in trans, in a late-post assembly maturation step, perhaps in concert with NS5A, to confer infectivity to the HCV particle...
  19. pmc Interaction of the influenza a virus nucleocapsid protein with the viral RNA polymerase potentiates unprimed viral RNA replication
    Laura L Newcomb
    Institute for Cellular and Molecular Biology, University of Texas at Austin, 2500 Speedway, Austin, TX 78712, USA
    J Virol 83:29-36. 2009
    ....
  20. ncbi Disrupting the association of hepatitis C virus core protein with lipid droplets correlates with a loss in production of infectious virus
    Steeve Boulant
    MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK
    J Gen Virol 88:2204-13. 2007
    ..Taken together, our findings indicate that lipid droplet association by core is connected to virus production...
  21. pmc Targeting of hepatitis C virus core protein to mitochondria through a novel C-terminal localization motif
    Bjorn Schwer
    Department of Molecular Virology, University of Heidelberg, Heidelberg, Germany
    J Virol 78:7958-68. 2004
    ..The location of the core protein in the outer mitochondrial membrane suggests that it could modulate apoptosis or lipid transfer, both of which are associated with this subcellular compartment, during HCV infection...
  22. pmc A PPxY motif within the VP40 protein of Ebola virus interacts physically and functionally with a ubiquitin ligase: implications for filovirus budding
    R N Harty
    Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, 3800 Spruce Street, Philadelphia, PA 19104 6049, USA
    Proc Natl Acad Sci U S A 97:13871-6. 2000
    ..Last, these studies imply that VP40 likely plays an important role in filovirus budding, and that budding of retroviruses, rhabdoviruses, and filoviruses may proceed via analogous mechanisms...
  23. ncbi Importin alpha nuclear localization signal binding sites for STAT1, STAT2, and influenza A virus nucleoprotein
    Krister Melen
    Laboratory of Infectious Disease Immunology, Department of Microbiology, National Public Health Institute, FIN 00300 Helsinki, Finland
    J Biol Chem 278:28193-200. 2003
    ....
  24. pmc Hepatitis C virus core protein decreases lipid droplet turnover: a mechanism for core-induced steatosis
    Charles Harris
    Gladstone Institute of Cardiovascular Disease, University of California, San Francisco, California 94158, USA
    J Biol Chem 286:42615-25. 2011
    ..Our results support a bipartite model in which core first requires DGAT1 to gain access to LDs, and then LD-localized core interferes with triglyceride turnover, thus stabilizing lipid droplets and leading to steatosis...
  25. ncbi Vesicular release of ebola virus matrix protein VP40
    J Timmins
    EMBL, 6 rue Jules Horowitz, 38042 Grenoble, France
    Virology 283:1-6. 2001
    ..Together, these results provide strong evidence that Ebola virus VP40 is sufficient for virus assembly and budding from the plasma membrane...
  26. pmc Contributions of two nuclear localization signals of influenza A virus nucleoprotein to viral replication
    Makoto Ozawa
    Division of Virology, Institute of Medical Science, University of Tokyo, Shirokanedai, Tokyo 108 8639, Japan
    J Virol 81:30-41. 2007
    ..A virus with nonfunctional NLS2 could not be generated. Thus, the bipartite NLS, but not the unconventional NLS, of NP is essential for influenza A virus replication...
  27. pmc Hepatitis C virus egress and release depend on endosomal trafficking of core protein
    Chao Kuen Lai
    Institute of Molecular Biology, Academia Sinica, Taipei 115, Taiwan
    J Virol 84:11590-8. 2010
    ..We also observed that this way is independent of that of the internalization of endocytosed virus particles during virus entry...
  28. pmc NP body domain and PB2 contribute to increased virulence of H5N1 highly pathogenic avian influenza viruses in chickens
    Tatsuya Tada
    Research Team for Zoonotic Diseases, National Institute of Animal Health, National Agriculture and Food Research Organization, 3 1 5 Kannondai, Tsukuba, Ibaraki 305 0856, Japan
    J Virol 85:1834-46. 2011
    ....
  29. pmc Increased intrahepatic cyclooxygenase 2, matrix metalloproteinase 2, and matrix metalloproteinase 9 expression is associated with progressive liver disease in chronic hepatitis C virus infection: role of viral core and NS5A proteins
    O Nunez
    Instituto de Hepatología Clínica Experimental y Trasplante Hepático, Hospital Universitario Gregorio Marañón Santa Cristina, Madrid, Spain
    Gut 53:1665-72. 2004
    ..Cyclooxygenase 2 (COX-2) and matrix metalloproteinases (MMPs) have been implicated in tissue injury and fibrogenesis in animal models but little is known regarding their role in hepatitis C virus (HCV) related liver disease in humans...
  30. ncbi Hepatitis C virus NS5A colocalizes with the core protein on lipid droplets and interacts with apolipoproteins
    Stephanie T Shi
    Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, Los Angeles, California 90033, USA
    Virology 292:198-210. 2002
    ....
  31. pmc Alanine scanning of the hepatitis C virus core protein reveals numerous residues essential for production of infectious virus
    Catherine L Murray
    Laboratory of Virology and Infectious Disease, Center for the Study of Hepatitis C, The Rockefeller University, 1230 York Ave, New York, NY 10021, USA
    J Virol 81:10220-31. 2007
    ....
  32. pmc Essential role of domain III of nonstructural protein 5A for hepatitis C virus infectious particle assembly
    Nicole Appel
    Department of Molecular Virology, University of Heidelberg, Heidelberg, Germany
    PLoS Pathog 4:e1000035. 2008
    ..Since domain III of NS5A is one of the most variable regions in the HCV genome, the results suggest that viral isolates may differ in their level of virion production and thus in their level of fitness and pathogenesis...
  33. pmc Regulation of antinucleoprotein IgG by systemic vaccination and its effect on influenza virus clearance
    Mark W Lamere
    Department of Medicine, Division of Allergy, Immunology and Rheumatology, University of Rochester Medical Center, Rochester, New York 14642, USA
    J Virol 85:5027-35. 2011
    ..The high conservation of NP antigen and the known longevity of antibody responses suggest that the antiviral activity of anti-NP IgG may provide a critically needed component of a universal influenza vaccine...
  34. pmc Ebola virus VP40 drives the formation of virus-like filamentous particles along with GP
    Takeshi Noda
    Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060 0818, Japan
    J Virol 76:4855-65. 2002
    ..These results demonstrated the central role of VP40 in formation of the filamentous structure of Ebola virions and may suggest an interaction between VP40 and GP in morphogenesis...
  35. ncbi An in vitro model of hepatitis C virus genotype 3a-associated triglycerides accumulation
    Karim Abid
    Division of Gastroenterology and Hepatology, University Hospital, 24 Rue Micheli du Crest, CH 1211 Geneva, Switzerland
    J Hepatol 42:744-51. 2005
    ..To extend these observations, we established an in vitro model expressing the HCV core of different genotypes...
  36. pmc Contribution of ebola virus glycoprotein, nucleoprotein, and VP24 to budding of VP40 virus-like particles
    Jillian M Licata
    Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, 3800 Spruce St, Philadelphia, PA 19104 6049, USA
    J Virol 78:7344-51. 2004
    ..the C-terminal 50 amino acids of NP may be important for interacting with and enhancing release of VP40 VLPs. These findings provide a more complete understanding of the role of VP40 and additional Ebola virus proteins during budding...
  37. ncbi Vaccination focusing immunity on conserved antigens protects mice and ferrets against virulent H1N1 and H5N1 influenza A viruses
    Graeme E Price
    Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Rockville, MD 20852, USA
    Vaccine 27:6512-21. 2009
    ..These findings demonstrate potent protection by vaccination highly focused on conserved antigens and identify immune response measures in mice that differed among vaccinations and correlated with outcome...
  38. pmc Differential antigen presentation regulates the changing patterns of CD8+ T cell immunodominance in primary and secondary influenza virus infections
    Sherry R Crowe
    Trudeau Institute, P O Box 59, Saranac Lake, NY 12983, USA
    J Exp Med 198:399-410. 2003
    ..Thus, differential antigen presentation impacts both the specificity of the T cell response and the efficacy of peptide-based vaccination strategies...
  39. ncbi Hepatitis C virus: viral proteins on the move
    John McLauchlan
    MRC Virology Unit, Church Street, Glasgow, UK
    Biochem Soc Trans 37:986-90. 2009
    ..Thus HCV may utilize the role played by LDs in the formation of lipoprotein particles as part of its life cycle and access this pathway by direct interaction of viral components with these intracellular storage organelles...
  40. pmc Proteasome activator PA28gamma-dependent nuclear retention and degradation of hepatitis C virus core protein
    Kohji Moriishi
    Research Center for Emerging Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Suita Shi, Osaka 565 0871, Japan
    J Virol 77:10237-49. 2003
    ..Overexpression of PA28gamma enhanced the proteolysis of the HCV core protein. Thus, the nuclear retention and stability of the HCV core protein is regulated via a PA28gamma-dependent pathway through which HCV pathogenesis may be exerted...
  41. ncbi A functional link between the actin cytoskeleton and lipid rafts during budding of filamentous influenza virions
    Martha Simpson-Holley
    Division of Virology, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, United Kingdom
    Virology 301:212-25. 2002
    ..We propose that the actin cytoskeleton is required to maintain the correct organisation of lipid rafts for incorporation into budding viral filaments...
  42. pmc Thermostability/infectivity defect caused by deletion of the core protein V gene in human adenovirus type 5 is rescued by thermo-selectable mutations in the core protein X precursor
    Hideyo Ugai
    Division of Human Gene Therapy, Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    J Mol Biol 366:1142-60. 2007
    ..The possible mechanism of the observed genetic suppression is discussed...
  43. ncbi Lipid droplets and hepatitis C virus infection
    John McLauchlan
    MRC Virology Unit, Church Street, Glasgow G11 5JR, UK
    Biochim Biophys Acta 1791:552-9. 2009
    ..The mechanisms involved in this process are not understood but evidence from patient-based studies and model systems suggests the involvement of both viral and host factors...
  44. ncbi HIV-1 and Ebola virus encode small peptide motifs that recruit Tsg101 to sites of particle assembly to facilitate egress
    J Martin-Serrano
    Aaron Diamond AIDS Research Center and The Rockefeller University, New York, New York, USA
    Nat Med 7:1313-9. 2001
    ..Because the Tsg101 is recruited by small, conserved viral sequence motifs, agents that mimic these structures are potential inhibitors of the replication of these lethal human pathogens...
  45. pmc Regulation of hepatitis C virion production via phosphorylation of the NS5A protein
    Timothy L Tellinghuisen
    The Scripps Research Institute, Scripps Florida, Jupiter, Florida, USA
    PLoS Pathog 4:e1000032. 2008
    ..The ability to uncouple virus production from RNA replication, as described herein, may be useful in understanding HCV assembly and may be therapeutically important...
  46. ncbi Dynamics of lipid droplets induced by the hepatitis C virus core protein
    Rodney K Lyn
    Steacie Institute for Molecular Sciences, National Research Council of Canada, Ottawa, Canada K1A 0R6
    Biochem Biophys Res Commun 399:518-24. 2010
    ..These dynamic processes ultimately aid HCV in propagating and the molecules and interactions involved represent novel targets for potential therapeutic intervention...
  47. ncbi Clinical significance of precore and core promoter mutations in genotype D hepatitis B-related chronic liver disease
    H Poustchi
    Medical Science, University Tehran, Iran
    J Viral Hepat 15:753-60. 2008
    ..001). The results indicate that in genotype D CHB, the presence of the A1762T-G1764A mutation was associated with more aggressive liver disease while the G1757A substitution was associated with protection from advanced disease...
  48. ncbi Human importin alpha and RNA do not compete for binding to influenza A virus nucleoprotein
    Sébastien Boulo
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Virology 409:84-90. 2011
    ..The complex human Impα5-NP binds RNA with the same affinity as wt NP alone, whereas engineered monomeric NP through point mutations binds RNA with a strongly reduced affinity...
  49. ncbi Hepatitis B virus core variants modify natural course of viral infection and hepatocellular carcinoma progression
    Feng Yu Sung
    Graduate Institute of Epidemiology, College of Public Health, National Taiwan University, Taipei, Taiwan
    Gastroenterology 137:1687-97. 2009
    ..We assessed the influence of genetic variants in the hepatitis B virus (HBV) core, which is a principal immunologic target, on the progression to hepatocellular carcinoma (HCC) in a cohort of 4841 male HBV carriers followed up for 16 years...
  50. ncbi Maturation of hepatitis C virus core protein by signal peptide peptidase is required for virus production
    Paul Targett-Adams
    MRC Virology Unit, Institute of Virology, Glasgow G11 5JR, United Kingdom
    J Biol Chem 283:16850-9. 2008
    ..Moreover, they offer compelling evidence for a function for an intramembrane protease to facilitate the association of core with viral genomes, thereby creating putative sites for assembly of nascent virus particles...
  51. ncbi Hepatitis C virus core and nonstructural proteins induce fibrogenic effects in hepatic stellate cells
    Ramó Bataller
    Department of Medicine and Biochemistry and Biophysics, University of North Carolina at Chapel Hill 27599 7038, USA
    Gastroenterology 126:529-40. 2004
    ..Hepatocytes secrete HCV proteins, which may interact with hepatic stellate cells (HSCs). Our aims were to investigate whether HCV proteins induce fibrogenic effects on HSCs...
  52. pmc Nuclear import of influenza A viral ribonucleoprotein complexes is mediated by two nuclear localization sequences on viral nucleoprotein
    Winco W H Wu
    Department of Zoology, University of British Columbia, Vancouver, BC, Canada
    Virol J 4:49. 2007
    ..These NP NLSs have been shown to mediate the nuclear import of recombinant NP molecules. However, it remains to be determined which NLS mediates the nuclear import of influenza vRNP complexes...
  53. pmc Hepatitis C virus impairs the induction of cytoprotective Nrf2 target genes by delocalization of small Maf proteins
    Monica Carvajal-Yepes
    Institute of Infection Medicine, Molecular Medical Virology, University of Kiel, D 24105 Kiel, Germany
    J Biol Chem 286:8941-51. 2011
    ....
  54. ncbi Pre-S deletion and complex mutations of hepatitis B virus related to advanced liver disease in HBeAg-negative patients
    Chien Hung Chen
    Division of Hepatogastroenterology, Department of Internal Medicine, Chang Gung Memorial Hospital Kaohsiung Medical Center, Kaohsiung, Taiwan
    Gastroenterology 133:1466-74. 2007
    ....
  55. pmc Localization of mammalian orthoreovirus proteins to cytoplasmic factory-like structures via nonoverlapping regions of microNS
    Cathy L Miller
    Dept of Veterinary Microbiology, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA
    J Virol 84:867-82. 2010
    ....
  56. pmc Domain 3 of hepatitis C virus core protein is sufficient for intracellular lipid accumulation
    Ravi Jhaveri
    Division of Infectious Diseases, Department of Pediatrics, Duke University Medical Center, Durham, North Carolina, USA
    J Infect Dis 200:1781-8. 2009
    ..In this study, we investigated the sufficiency of d3 to promote lipid accumulation, the role of HCV genotype in d3 lipid accumulation, and the subcellular distribution of d3...
  57. pmc Ubiquitin-independent degradation of hepatitis C virus F protein
    Kamile Yuksek
    Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, California 90033, USA
    J Virol 83:612-21. 2009
    ..The ability of the F protein to bind to alpha3 raises the possibility that the HCV F protein may regulate protein degradation in cells...
  58. ncbi Basal core promoter mutations of hepatitis B virus increase the risk of hepatocellular carcinoma in hepatitis B carriers
    Jia Horng Kao
    Graduate Institute of Clinical Medicine, National Taiwan University College of Medicine, Taipei, Taiwan
    Gastroenterology 124:327-34. 2003
    ..We investigated the prevalence of T1762/A1764 basal core promoter mutant in a cohort of 250 genotype B- or C-infected HBV carriers with different stages of liver disease to clarify a possible role for this mutant in hepatocarcinogenesis...
  59. pmc Structure of the influenza virus A H5N1 nucleoprotein: implications for RNA binding, oligomerization, and vaccine design
    Andy Ka Leung Ng
    Department of Biochemistry and Center for Protein Science and Crystallography, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China
    FASEB J 22:3638-47. 2008
    ..Our study of H5N1 NP provides insight into the oligomerization interface and the RNA-binding groove, which are attractive drug targets, and it identifies the epitopes that might be used for universal vaccine development...
  60. pmc Kinetic analysis of the nucleic acid chaperone activity of the hepatitis C virus core protein
    Kamal Kant Sharma
    Laboratorie de Biophotonique et Pharmacologie, UMR 7213 CNRS, Faculte de Pharmacie, Universite de Strasbourg, 74, route du Rhin, 67401, Illkirch, Cedex, France
    Nucleic Acids Res 38:3632-42. 2010
    ..The three peptides operate similarly, confirming that the core chaperone properties are mostly supported by its basic clusters...
  61. ncbi An unconventional NLS is critical for the nuclear import of the influenza A virus nucleoprotein and ribonucleoprotein
    Jerome F Cros
    Department of Microbiology, Mount Sinai School of Medicine, 1 Gustave L Levy Place, New York, NY 10029, USA
    Traffic 6:205-13. 2005
    ..Finally, viral replication could be specifically inhibited by a membrane-permeable peptide containing the unconventional NLS, confirming the crucial role of this signal during the replicative cycle of the virus...
  62. ncbi Vaccination of pigs with a DNA construct expressing an influenza virus M2-nucleoprotein fusion protein exacerbates disease after challenge with influenza A virus
    Paul P Heinen
    Division of Mammalian Virology, Institute for Animal Science and Health ID Lelystad, PO Box 65, 8200 AB Lelystad, The Netherlands
    J Gen Virol 83:1851-9. 2002
    ..Thus, clinical signs after infection should be observed closely in further studies using M2e as an immunogen and caution should be exercised in using M2e in humans...
  63. pmc Upregulation of major histocompatibility complex class I on liver cells by hepatitis C virus core protein via p53 and TAP1 impairs natural killer cell cytotoxicity
    Kerstin Herzer
    Division of Immunogenetics, German Cancer Research Center DKFZ, D 69120 Heidelberg, Germany
    J Virol 77:8299-309. 2003
    ..These results demonstrate a way in which HCV avoids recognition by NK cells that may contribute to the establishment of a chronic infection...
  64. ncbi Hepatitis C virus contributes to hepatocarcinogenesis by modulating metabolic and intracellular signaling pathways
    Kazuhiko Koike
    Department of Infectious Diseases, Internal Medicine, Graduate School of Medicine, University of Tokyo, Bunkyo ku, Tokyo, Japan
    J Gastroenterol Hepatol 22:S108-11. 2007
    ..Such a scenario, 'non-Vogelstein-type' carcinogenesis, would explain the rare feature of hepatocarcinogenesis in HCV infection, the extraordinarily high incidence and the multicentric nature of HCC development...
  65. ncbi The matrix protein VP40 from Ebola virus octamerizes into pore-like structures with specific RNA binding properties
    F Xavier Gomis-Ruth
    European Molecular Biology Laboratory EMBL, 6 rue Jules Horowitz, 38042, Grenoble, France
    Structure 11:423-33. 2003
    ..SDS-resistant octameric VP40 is also found in Ebola virus-infected cells, which suggests that VP40 has an additional function in the life cycle of the virus besides promoting virus assembly and budding off the plasma membrane...
  66. ncbi Ebola virus matrix protein VP40 interaction with human cellular factors Tsg101 and Nedd4
    Joanna Timmins
    European Molecular Biology Laboratory, 6 rue Jules Horowitz, 38042 Grenoble, France
    J Mol Biol 326:493-502. 2003
    ..These results suggest that hNedd4 and Tsg101 may play complimentary roles at a late stage of the assembly process, by recruiting cellular factors of two independent pathways to the site of budding at the plasma membrane...
  67. ncbi Orthoreovirus and Aquareovirus core proteins: conserved enzymatic surfaces, but not protein-protein interfaces
    Jonghwa Kim
    Department of Molecular and Cellular Biology, Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02115, USA
    Virus Res 101:15-28. 2004
    ..These findings provide further evidence that Orthoreoviruses and Aquareoviruses have diverged from a common ancestor and contribute to a growing understanding of the functions of the core proteins in viral mRNA synthesis...
  68. ncbi Both matrix proteins of Ebola virus contribute to the regulation of viral genome replication and transcription
    T Hoenen
    Institute for Virology, Philipps University Marburg, Marburg, Germany
    Virology 403:56-66. 2010
    ..Further, we show for the first time that VP40 is also involved in regulating these processes. These functions are conserved among EBOV species and, in the case of VP40, independent of its budding or RNA-binding functions...
  69. pmc Functional analysis of the influenza virus H5N1 nucleoprotein tail loop reveals amino acids that are crucial for oligomerization and ribonucleoprotein activities
    Wai Hon Chan
    Department of Biochemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China
    J Virol 84:7337-45. 2010
    ..The work presented here provides information for the design of NP inhibitors for combating influenza virus infection...
  70. ncbi Hepatitis C virus core protein genotype 3a increases SOCS-7 expression through PPAR-{gamma} in Huh-7 cells
    Valerio Pazienza
    Gastroenterology Unit, IRCCS Casa Sollievo della Sofferenza Hospital, San Giovanni Rotondo FG, Italy
    J Gen Virol 91:1678-86. 2010
    ..This is the first report proposing a molecular mechanism through which the HCV core protein (genotype 3a) modulates SOCS-7 expression...
  71. ncbi HCV persistence and immune evasion in the absence of memory T cell help
    Arash Grakoui
    Center for the Study of Hepatitis C, Rockefeller University, New York, NY 10021, USA
    Science 302:659-62. 2003
    ....
  72. pmc Molecular determinants for subcellular localization of hepatitis C virus core protein
    Ryosuke Suzuki
    Department of Virology II, National Institute of Infectious Diseases, 1 23 1 Toyama, Shinjuku ku, Tokyo, Japan 162 8640
    J Virol 79:1271-81. 2005
    ..Differences in the cellular trafficking of HCV core protein, achieved and maintained by multiple targeting functions as mentioned above, may in part regulate the diverse range of biological roles of the core protein...
  73. pmc The influenza A virus NS1 protein interacts with the nucleoprotein of viral ribonucleoprotein complexes
    Nicole C Robb
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, United Kingdom
    J Virol 85:5228-31. 2011
    ..The region of NS1 that binds NP was mapped to the RNA-binding domain...
  74. pmc Involvement of template-activating factor I/SET in transcription of adenovirus early genes as a positive-acting factor
    Hirohito Haruki
    Department of Infection Biology, Graduate School of Comprehensive Human Sciences and Institute of Basic Medical Sciences, University of Tsukuba, 1 1 1 Tennodai, Tsukuba 305 8575, Japan
    J Virol 80:794-801. 2006
    ..These results provide evidence that TAF-I plays an important role in the early stages of the adenovirus infection cycle...
  75. ncbi Proteomic profiling of lipid droplet proteins in hepatoma cell lines expressing hepatitis C virus core protein
    Shigeko Sato
    Department of Biochemistry and Cell Biology and Department of Virology II, National Institute of Infectious Diseases, Tokyo 162 8640
    J Biochem 139:921-30. 2006
    ..These results suggest that lipid droplets containing HCV core protein may participate in the RNA metabolism of the host and/or HCV, affecting the pathopoiesis and/or virus replication/production in HCV-infected cells...
  76. pmc Intramembrane proteolysis and endoplasmic reticulum retention of hepatitis C virus core protein
    Kiyoko Okamoto
    Research Center for Emerging Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamadaoka, Suita Shi, Osaka 565 0871, Japan
    J Virol 78:6370-80. 2004
    ..These results indicate that Leu(139), Val(140), and Leu(144) in core protein play crucial roles in the ER retention and SPP cleavage of HCV core protein...
  77. pmc Intramembrane processing by signal peptide peptidase regulates the membrane localization of hepatitis C virus core protein and viral propagation
    Kiyoko Okamoto
    Department of Molecular Virology, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    J Virol 82:8349-61. 2008
    ..These results suggest that intramembrane processing of HCV core protein by SPP is required for the localization of the HCV core protein in the DRM and for viral propagation...
  78. pmc Regulation of hepadnavirus reverse transcription by dynamic nucleocapsid phosphorylation
    Suresh H Basagoudanavar
    Department of Microbiology and Immunology H107, The Penn State University College of Medicine, 500 University Dr, Hershey, PA 17033, USA
    J Virol 81:1641-9. 2007
    ..Thus, the reversible phosphorylation of the nucleocapsids regulates the ordered progression of reverse transcription...
  79. ncbi RNA interference effectively inhibits mRNA accumulation and protein expression of hepatitis C virus core and E2 genes in human cells
    Min Liu
    Department of Microbiology, State Key Laboratory of Medical Immunology, Second Military Medical University, Shanghai, China
    Biosci Biotechnol Biochem 70:2049-55. 2006
    ..This may provide an approach for the development of novel prophylactic or therapeutic agents for HCV infection...
  80. ncbi Characterization of cell lines allowing tightly regulated expression of hepatitis C virus core protein
    D Moradpour
    Molecular Hepatology Laboratory, Massachusetts General Hospital Cancer Center, Harvard Medical School, Charlestown 02129, USA
    Virology 222:51-63. 1996
    ..These cell lines represent important tools to investigate structural and functional properties of HCV core protein and may be useful to evaluate gene therapeutic strategies against HCV in a cellular system...
  81. ncbi Pathogenesis of lipid metabolism disorder in hepatitis C: polyunsaturated fatty acids counteract lipid alterations induced by the core protein
    Hideyuki Miyoshi
    Department of Internal Medicine, Graduate School of Medicine, University of Tokyo, Tokyo, Japan
    J Hepatol 54:432-8. 2011
    ..Experimental studies have revealed that the core protein of hepatitis C virus (HCV) induces steatosis...
  82. ncbi Up-regulation of fatty acid synthase promoter by hepatitis C virus core protein: genotype-3a core has a stronger effect than genotype-1b core
    Candice Jackel-Cram
    Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Saskatchewan, Canada
    J Hepatol 46:999-1008. 2007
    ..Hepatitis C virus genotype-3a (HCV-3a) is directly linked to steatosis development. We studied the effects of HCV-3a core protein on the promoter activity of fatty acid synthase (FAS), a major enzyme involved in de novo lipid synthesis...
  83. pmc Influenza a virus polymerase is an integral component of the CPSF30-NS1A protein complex in infected cells
    Rei Lin Kuo
    Institute for Cellular and Molecular Biology, University of Texas at Austin, 78712, USA
    J Virol 83:1611-6. 2009
    ....
  84. pmc Reovirus nonstructural protein mu NS recruits viral core surface proteins and entering core particles to factory-like inclusions
    Teresa J Broering
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Virol 78:1882-92. 2004
    ..These findings expand a model for how viral and cellular components are recruited to the viral factories in infected cells and provide further evidence for the central but distinct roles of viral proteins mu NS and mu 2 in this process...
  85. pmc Testing the balanced electrostatic interaction hypothesis of hepatitis B virus DNA synthesis by using an in vivo charge rebalance approach
    Pong Kian Chua
    Institute for Human Infections and Immunology, Department of Pathology, and Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas 77555 0609, USA
    J Virol 84:2340-51. 2010
    ..These results support the concept that balanced electrostatic interaction is important during the viral life cycle...
  86. pmc Age-associated decline in T cell repertoire diversity leads to holes in the repertoire and impaired immunity to influenza virus
    Eric J Yager
    The Trudeau Institute, Saranac Lake, NY 12983, USA
    J Exp Med 205:711-23. 2008
    ..These observations have important implications for the design of vaccine strategies for the elderly...
  87. pmc Mutational analysis of conserved amino acids in the influenza A virus nucleoprotein
    Zejun Li
    Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    J Virol 83:4153-62. 2009
    ..The respective conserved amino acids in NP may thus be critical for the assembly and/or incorporation of sets of eight vRNA segments...
  88. pmc Identification of basic amino acids at the N-terminal end of the core protein that are crucial for hepatitis C virus infectivity
    Khaled Alsaleh
    Inserm U1019, CNRS UMR 8204, Center for Infection and Immunity of Lille, Institut de Biologie de Lille, F 59021 Lille, France
    J Virol 84:12515-28. 2010
    ..Together, these data indicate that R50, K51, R59, and R62 residues play a major role in the formation of infectious viral particles at a post-nucleocapsid assembly step...
  89. pmc Nuclear export and import of human hepatitis B virus capsid protein and particles
    Hung Cheng Li
    Institute of Biochemical Sciences, National Taiwan University, Taipei, Taiwan
    PLoS Pathog 6:e1001162. 2010
    ..In addition to the revised map of NLS, our results suggest that HBc could shuttle rapidly between nucleus and cytoplasm via a novel TAP-dependent NES...
  90. ncbi West Nile virus core protein; tetramer structure and ribbon formation
    Terje Dokland
    Institute of Molecular and Cell Biology, Singapore, Republic of Singapore
    Structure 12:1157-63. 2004
    ..The C protein structure contains four alpha helices and forms dimers that are organized into tetramers. The tetramers form extended filamentous ribbons resembling the stacked alpha helices seen in HEAT protein structures...
  91. pmc NS3 helicase domains involved in infectious intracellular hepatitis C virus particle assembly
    Yinghong Ma
    Center for Hepatitis Research, The University of Texas Medical Branch at Galveston, 301 University Boulevard, Galveston, TX 77555 1073, USA
    J Virol 82:7624-39. 2008
    ..These data reveal a previously unsuspected role for the NS3 helicase in early virion morphogenesis and provide a new perspective on HCV assembly...
  92. ncbi Transcription releases protein VII from adenovirus chromatin
    Jiangning Chen
    Department of Microbiology, University of Virginia Health System, PO Box 800734, Charlottesville, VA 22908 0734, USA
    Virology 369:411-22. 2007
    ..Our data support a model in which early gene transcription results in a change in the structure of the viral chromatin...
  93. pmc Hepatitis C virus structural proteins impair dendritic cell maturation and inhibit in vivo induction of cellular immune responses
    Pablo Sarobe
    Division of Hepatology and Gene Therapy, Fundación para la Investigación Médica Aplicada FIMA, University of Navarra, 31008 Pamplona, Spain
    J Virol 77:10862-71. 2003
    ..These findings are relevant for the design of therapeutic vaccination strategies in HCV-infected patients...
  94. ncbi HBV core particles as a carrier for B cell/T cell epitopes
    P Pumpens
    Biomedical Research and Study Center, University of Latvia, Riga, Latvia
    Intervirology 44:98-114. 2001
    ..Combination of numerous experimental data on epitope display with the precise structural information enables a knowledge-based design of diagnostic, and vaccine and gene therapy tools on the basis of the HBc particles...
  95. ncbi The lipid droplet binding domain of hepatitis C virus core protein is a major determinant for efficient virus assembly
    Anna Shavinskaya
    Department of Molecular Virology, University of Heidelberg, 69120 Heidelberg, Germany
    J Biol Chem 282:37158-69. 2007
    ..Taken together, our results demonstrate that the binding strength of the D2 domain of core for LDs is crucial for determining the efficiency of virus assembly...
  96. ncbi Augmentation of primary influenza A virus-specific CD8+ T cell responses in aged mice through blockade of an immunoinhibitory pathway
    Lauren Dimenna
    Wistar Institute, Philadelphia, PA 19104, USA
    J Immunol 184:5475-84. 2010
    ..Our results show that the vaccine expressing a fusion protein of NP and glycoprotein D induces significantly higher NP-specific CD8(+) T cell responses in young and aged mice compared with the vaccine expressing NP only...
  97. pmc A role for transportin in the nuclear import of adenovirus core proteins and DNA
    Clemence E Hindley
    Department of Cellular and Molecular Medicine, School of Medical Sciences, University of Bristol, Bristol, UK
    Traffic 8:1313-22. 2007
    ..Our results implicate transportin as a key import receptor for the nuclear localization of adenovirus core...
  98. ncbi [Efficient soluble expression and purification of influenza A nucleoprotein in Escherichia coli]
    Bao Ying Huang
    National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China
    Bing Du Xue Bao 27:50-7. 2011
    ..These results suggest that the codon-optimized influenza A virus NP gene can be efficiently expressed in E. coli and the expressed NP protein with specific immune reactivity could be purified from the supernatant of bacterial lysate...
  99. pmc Protection against mouse and avian influenza A strains via vaccination with a combination of conserved proteins NP, M1 and NS1
    Oleg P Zhirnov
    D I Ivanovsky Institute of Virology RAMS, Moscow, Russia
    Influenza Other Respi Viruses 1:71-9. 2007
    ..At the same time, the efficacy of immunization schemes designed along these lines and involving internal influenza proteins, mostly NP and M1, has not been sufficient...
  100. ncbi T cell dysfunction by hepatitis C virus core protein involves PD-1/PDL-1 signaling
    Zhi Q Yao
    Department of Internal Medicine, James H Quillen College of Medicine, East Tennessee State University, Johnson City, Tennessee 37614, USA
    Viral Immunol 20:276-87. 2007
    ..This upregulation of the PD-1 and PDL-1 pathway in humans represents a novel and perhaps common mechanism by which a virus usurps host machinery to facilitate persistence...
  101. ncbi Association between mutations in the core region of hepatitis C virus genotype 1 and hepatocellular carcinoma development
    Shingo Nakamoto
    Department of Medicine and Clinical Oncology, Graduate School of Medicine, Chiba University, 1 8 1 Inohana, Chuo Ward, Chiba City, Chiba 260 8670, Japan
    J Hepatol 52:72-8. 2010
    ..To determine whether amino acid mutations in the core region of hepatitis C virus (HCV) genotype 1 are associated with response to interferon (IFN) therapy and development of hepatocellular carcinoma (HCC)...

Research Grants66

  1. Murine Model of HCV-Associated Human Liver Cancer
    Lishan Su; Fiscal Year: 2013
    ....
  2. Effects of Ethanol on Proteasome-HCV Core Protein Interactions
    NATALIA ALEKSANDR OSNA; Fiscal Year: 2010
    ..We envision that this research will potentially have therapeutic applications, helping to improve chronic hepatitis C treatment and vaccine development ..
  3. BIOLOGY OF TRABECULAR RESPONSE TO LASER TRABECULOPLASTY
    Ted S Acott; Fiscal Year: 2012
    ..These studies are focused at understanding the molecular nature of the aqueous humor outflow pathway, which is normally responsible for avoiding glaucoma and which becomes obstructed to produce glaucoma. ..
  4. 10-Theory and Experiment for Genome Packaging in HBV Nucleocapsids
    JIANZHONG contact WU; Fiscal Year: 2010
    ....
  5. REGULATORY INTERACTIONS BETWEEN TUMOR VIRUSES AND CELLS
    Michael R Botchan; Fiscal Year: 2013
    ..The signaling events that control the action of replication proteins, will be provide insights into the networks that control cell cycle progression and should help in cancer therapy and diagnosis...
  6. Metabolic Engineering of Chinese Hamster Ovary Cells for Production of Heparin
    Susan T Sharfstein; Fiscal Year: 2011
    ..Scale-up the production and recovery of CHO cell metabolically engineered heparin while maintaining chemical and bioequivalence. ..
  7. gC1q-R/P33: Structure-function and role in inflammation
    Berhane Ghebrehiwet; Fiscal Year: 2012
    ..abstract_text> ..
  8. SEQUENCING THE POLYSACCHARIDE COMPONENT OF PROTEOGLYCANS
    Robert J Linhardt; Fiscal Year: 2012
    ..Currently it is difficult to analyze and not possible to sequence these complex polysaccharides. This has prevented a firm understanding of the structure- activity relationship of this important class of biomacromolecules. ..
  9. Cyclic Nucleotide-Activated Calcium Channels in Sperm
    Dejian Ren; Fiscal Year: 2010
    ..Results from this study will allow us to understand the ion mechanism underlying sperm motility and may lay the foundation for the future development of drugs for infertility treatment and for male contraceptives. ..
  10. Molecular Modulation of Virus Capsid Assembly
    Adam Zlotnick; Fiscal Year: 2013
    ..These independent aims will move assembly effectors from the subject of purely academic investigation towards clinical application. ..
  11. Mechanism of Hepatitis C Virus Core Protein Domain 3 in Hepatocyte Lipid Accum
    Ravi R Jhaveri; Fiscal Year: 2010
    ..It is hypothesized that steatosis, or fatty liver, that is observed with considerable frequency in chronic HCV infection is the accumulation over time of excess lipid after the reproductive requirements of HCV are met. ..
  12. Internal Dynamics of the Postsynaptic Density
    Thomas A Blanpied; Fiscal Year: 2013
    ..Further, they will provide an important platform on which to test hypotheses regarding the molecular basis of disorders that disrupt synaptic transmission. ..
  13. The Pathogenesis of Steatosis in Hepatitis C Genotype 3 Infection
    Ravi Jhaveri; Fiscal Year: 2009
    ..Results from this work may improve counselling of HCV patients with steatosis and may facilitate efforts to identify novel targets for anti-viral development. ..
  14. Role of HCV in aberrant APC activation/function
    Young S Hahn; Fiscal Year: 2013
    ..We believe that results of these studies will provide a basis fr the rational design of vaccines and novel therapeutics against HCV infection in humans by bypassing the immunosuppressive effect of HCV. ..
  15. Mechanisms and Functions of Core-induced Lipid Droplet Accumulation in Hepatitis
    MELANIE MARIA OTT; Fiscal Year: 2010
    ..These studies are directly relevant to HCV pathogenesis and may contribute to the development of novel antiviral drugs that will address public need. ..
  16. XLF in double-strand break repair and chemo/radiosensitization
    Lawrence F Povirk; Fiscal Year: 2013
    ..Based on their unique mode of action, these peptides or subsequent derivatives thereof may be clinically useful in sensitizing tumor cells for radio- and chemotherapy. ..
  17. Molecular determinants of hepatitis C virus assembly
    Brett D Lindenbach; Fiscal Year: 2013
    ..Our studies characterize the virus particle assembly process, which will provide new avenues to target HCV and thereby prevent or treat disease. ..
  18. A Novel Method of Generating Hepatitis C Virus-Like Particles using Lentivirus
    Boro Dropulic; Fiscal Year: 2009
    ..The technology can be used in multiple formats, and in particular related to this RFA it can be used for rapid production of virus-like particles from human cells. ..
  19. Hepatitis B virus genotypes B and C
    Shuping Tong; Fiscal Year: 2013
    ..Our rich experience and proven track record in characterizing biological properties of HBV genetic variants will ensure successful completion of the proposed study. ..
  20. Structure/Function Relationship of The Lumican Gene
    Winston W Kao; Fiscal Year: 2010
    ..g., persistent and excessive inflammation, corneal transparency of injured cornea, improved wound healing of diabetes, etc. ..
  21. Control of HBV replication at the step of core and P protein translation
    Jisu Li; Fiscal Year: 2013
    ..Our studies will clarify translational control of core and P protein expression, and help understand how different HBV genetic variants avoid deviating from an optimal core/P protein ratio required for efficient genome replication. ..
  22. Ultrasensitive Detection of Hepatitis Viruses by Immunoassay Amplification
    Boris Rotman; Fiscal Year: 2013
    ..Project Description ..
  23. A Therapeutic Vaccine for Chronic Hepatitis B
    David R Milich; Fiscal Year: 2013
    ..In Aim 2 preclinical development of the WHcAg-PreS1 vaccine will be pursued including cGMP manufacturing and pharm/tox studies. ..
  24. Heparan sulfate proteoglycans as critical regulators of brain cancer malignancy
    Joanna Phillips; Fiscal Year: 2013
    ..Successful completion of these studies provides a preclinical basis to study agents that target HSPGs as a novel therapeutic option in malignant brain cancer. ..
  25. Virus-gene interactions during hepatic carcinogenesis
    Xin Chen; Fiscal Year: 2012
    ..The murine models generated from the proposed study will significantly benefit HCC research community and provide valuable in vivo models to study novel strategies for HCC prevention and treatment. ..
  26. Biogenesis of H/ACA Ribonucleoproteins
    U Thomas Meier; Fiscal Year: 2013
    ....
  27. Extracellular determinants of polycystic kidney disease severity
    Myron E Hinsdale; Fiscal Year: 2013
    ..The proposed research is significant because the identification of genetic modifiers and understanding their impact on clinical variation is expected to advance individualized ARPKD patient treatment and prognostication. ..
  28. Biogenesis and Function of Guide RNAs
    RUSLAN AFASIZHEV; Fiscal Year: 2012
    ....
  29. Understanding and Targeting the DNA Repair Network in Cancer
    Guang Peng; Fiscal Year: 2013
    ..abstract_text> ..
  30. Understanding and Targeting the DNA Repair Network in Cancer
    Guang Peng; Fiscal Year: 2012
    ....
  31. Syndecan-1 in Lung Repair
    Peter Chen; Fiscal Year: 2013
    ....
  32. The Biophysics of Virus Capsid Assembly
    Adam Zlotnick; Fiscal Year: 2013
    ..By considering Cp as a molecular machine, we will be able to identify regulatory elements in the structure that may be targets for interfering with assembly and thus the HBV lifecycle. ..
  33. Molecular Mechanisms Regulating Intercellular Transit of TGF-beta
    Tina L Gumienny; Fiscal Year: 2013
    ....
  34. COLLAGEN FIBRILLOGENESIS AND CORNEAL DEVELOPMENT
    David E Birk; Fiscal Year: 2013
    ..Definition of the regulatory steps in corneal-specific matrix assembly provides the foundation to further our understanding of corneal repair/regeneration, pathobiologies and the manipulation of these processes. ..
  35. Role of Adenovirus Core Proteins in Innate Signaling and Viral Genome Packaging
    Patrick Hearing; Fiscal Year: 2013
    ..There is no virus-specific therapy for Ad infection. Thus, it has become increasingly important to fully understand the Ad replication cycle in order to foster the development of antiviral therapies. ..
  36. Molecular Biology and Genetics of Human Tumor Viruses
    Paul F Lambert; Fiscal Year: 2013
    ..abstract_text> ..
  37. EARLY DIAGNOSIS OF PANCREATIC CANCER
    Michael A Hollingsworth; Fiscal Year: 2013
    ....
  38. Matrix-assisted Craniofacial Bone Regeneration
    Mitsuo Yamauchi; Fiscal Year: 2012
    ..Towards this end, this study will explore a novel approach to efficient and cost-effective craniofacial bone regeneration based on a unique function of bone matrix molecule, biglycan. ..
  39. Mechanisms of Liver Disease Progression by Hepatitis C Virus
    Ranjit Ray; Fiscal Year: 2013
    ..Knowledge on the molecular determinants involved in HCV mediated disease progression will open avenues for novel therapeutic approach. ..
  40. Multiepitope circumsporozoite P.falciparum malaria subunit vaccine displayed on v
    FIDEL P ZAVALA; Fiscal Year: 2012
    ..falciparum malaria. ..
  41. PROTEOGLYCANS, SYNAPTIC PLASTICITY IN AGING AND DISEASE
    Paul E Gottschall; Fiscal Year: 2010
    ..Should synaptic remodeling be altered, due to diminished proteolytic cleavage of brevican, stimulation of proteolysis may be of therapeutic value. ..
  42. HIV-Hepatitis C Virus Interactions and Pathogenesis
    Raymond T Chung; Fiscal Year: 2010
    ..These studies will shed considerable light on HIV-HCV pathogenic interactions and offer a means of productively disrupting these interactions. ..
  43. SPECIFICITY OF DOMAIN INTERACTIONS IN CELL SIGNALING
    Mark A Lemmon; Fiscal Year: 2010
    ..In parallel, we will map orthologous interactions in mammalian cells, establishing a new paradigm for PH domains that resembles the role of SH2, and SH3 domains. ..
  44. Structure and mechanism of signal peptide peptidase
    Michael S Wolfe; Fiscal Year: 2010
    ..A structure of SPP would provide tremendous insight into the workings of an unusual new class of enzymes that are critical to both biology and medicine. ..
  45. The Mechanisms of Lipid Droplet Formation and Regulation
    Robert V Farese; Fiscal Year: 2013
    ....
  46. Role of immature DC in host defense against HCV
    Donald D Anthony; Fiscal Year: 2010
    ..abstract_text> ..
  47. Hepatitis C viral sensing by non-parenchymal liver cells (NPC)
    HUGO RAMON ROSEN; Fiscal Year: 2013
    ..The personnel involved in this application include graduate student, post-doctoral fellow and faculty. ..
  48. Injury-targeted decorin for systemic anti-fibrotic treatment
    Erkki Ruoslahti; Fiscal Year: 2011
    ..We have improved the activity of decorin by adding a homing signal that directs decorin to sites of injury, and propose here studies that will advance the targeted decorin toward the clinic. ..
  49. PROTEOGLYCAN SYNTHESIS IN MUTANT MOUSE SYSTEMS
    Nancy B Schwartz; Fiscal Year: 2013
    ..2) Elucidate the mechanism by which absence of CSPG core protein or CS chains affects IHH and FGF signaling throughout growth development. ..
  50. TYPE IV COLLAGEN IN MELANOMA CELL INVASION & METASTASIS
    James McCarthy; Fiscal Year: 1999
    ..In addition, these studies may provide a first generation of structurally constrained compounds that could be useful in metastasis management in vivo. ..
  51. Novel Approaches to Structure/Function Analyses of Heparan Sulfate in vivo
    HANNES ERICH BUELOW; Fiscal Year: 2010
    ..Understanding heparan sulfate structure function relationships may ultimately allow to interfere with deregulated cell-cell signaling during malignancies or host- microbe interactions. ..
  52. CHARACTERIZATION OF THE PEMPHIGUS FOLIACEUS ANTIGEN
    Grant Anhalt; Fiscal Year: 1993
    ....
  53. MOLECULAR STUDIES OF CORNEAL TRANSPARENCY
    John Hassell; Fiscal Year: 2003
    ....
  54. COMPLEX CARBOHYDRATES OF NERVOUS TISSUE
    RICHARD MARGOLIS; Fiscal Year: 2001
    ....
  55. PROTEOGLYCANS IN THE PATHOGENESIS OF ATHEROSCLEROSIS
    Gerald Berenson; Fiscal Year: 1992
    ..These studies will help to clarify the role of PG in the pathogenesis of atherosclerosis...
  56. TGFB AND GENE THERAPY IN SILICA INDUCED LUNG FIBROSIS
    Patricia Sime; Fiscal Year: 2005
    ..In conjunction with a formal education program me this should allow her to successfully develop as an independent investigator, while yielding new insights into the pathogenesis and treatment of fibrotic pathologies. ..
  57. NOVEL SEROTHERAPY STRATEGIES--PLASMA CELL MALIGNANCIES
    STEVEN TREON; Fiscal Year: 2004
    ..Analogous to our work with Muc-1, we will also attempt to identify other PCD selective targets by generating and examining mAbs specific to the plasma cell glycoform of CD138. ..
  58. Characterizing the function of Syndecan during CNS development
    Karl Johnson; Fiscal Year: 2009
    ..In addition, because mutations in Sdc have been linked to obesity in animals and humans, elucidating the mechanisms of Sdc function may also lead to new treatment strategies for obesity. ..
  59. BASEMENT MEMBRANE PROTEOGLYCANS
    JOHN COUCHMAN; Fiscal Year: 2001
    ..Roles for regulation by matrix and growth factors will be assessed. Genomic cloning of mouse bamacan is proposed, with emphasis on the 5' regions, directed towards understanding gene regulation as well as future targeted deletion. ..
  60. Host Partners and Virus Assembly
    Adam Zlotnick; Fiscal Year: 2010
    ..We will investigate how HBV interacts with two host proteins that are critical to HBV replication and may represent new targets for antiviral therapeutics. ..
  61. MOLECULAR MECHANISM OF MATRIX PROTEIN PHOSPHORYLATION
    CHOU WU; Fiscal Year: 1993
    ..These data will provide vital information regarding the significance of matrix protein phosphorylation in matrix-mediated mineralization of bone and dentin...
  62. CHONDROITIN SULFATE BIOSYNTHESIS CELLULAR LOCALIZATION
    Geetha Sugumaran; Fiscal Year: 2002
    ..These studies will provide fundamental information for examining the regulation and organization of proteochondroitin synthesis and will enable us to obtain necessary tools for future studies. ..
  63. IMMUNOMODULATORY ROLE OF HCV CORE ON AUTOIMMUNE HEPATITI
    YOUNG HAHN; Fiscal Year: 2001
    ..They will then characterize the intrahepatic lymphocytes and analyze the core-mediated liver injury in CD2/core transgenic mice. ..
  64. Heme Oxygenase-1 and Hepatitis C
    Warren Schmidt; Fiscal Year: 2006
    ..abstract_text> ..
  65. Structural and Functional Studies of Hepatitic C Core and NS4B Proteins
    Daniel Calarese; Fiscal Year: 2009
    ..Understanding the precise role of these proteins in the virus life cycle would not only advance knowledge in basic HCV biology, but would illuminate novel targets for therapeutic drug design. ..
  66. T CELL IMMUNE RESPONSE TO BREAST CARCINOMA
    Timothy Eberlein; Fiscal Year: 1999
    ..These CTL could be used in adoptive immunotherapy of cancer patients. Finally, synthetic tumor-derived peptides could be used in a vaccine-based treatment of patients with cancer. ..