- Generalized lacZ expression with the ROSA26 Cre reporter strain
Nat Genet 21:70-1. 1999
- The multifunctional NS1 protein of influenza A viruses
Benjamin G Hale
Centre for Biomolecular Sciences, University of St Andrews, St Andrews, Fife KY16 9ST, UK
J Gen Virol 89:2359-76. 2008
- A physical and regulatory map of host-influenza interactions reveals pathways in H1N1 infection
Sagi D Shapira
Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA
Cell 139:1255-67. 2009
During the course of a viral infection, viral proteins interact with an array of host proteins and pathways. Here, we present a systematic strategy to elucidate the dynamic interactions between H1N1 influenza and its human host...
- Replication of hepatitis C virus
Division of Gastroenterology and Hepatology, Centre Hospitalier Universitaire Vaudois, University of Lausanne, CH 1011 Lausanne, Switzerland
Nat Rev Microbiol 5:453-63. 2007
..These efforts have already translated into the identification of novel antiviral targets and the development of new therapeutic strategies, some of which are currently undergoing clinical evaluation...
- An efficient recombination system for chromosome engineering in Escherichia coli
Gene Regulation and Chromosome Biology Laboratory and Mouse Cancer Genetics Program, National Cancer Institute, Division of Basic Science, National Cancer Institute Frederick Cancer Research and Development Center, Frederick, MD 21702, USA
Proc Natl Acad Sci U S A 97:5978-83. 2000
..This system will be especially useful for the engineering of large bacterial plasmids such as those from bacterial artificial chromosome libraries...
- Distant Mimivirus relative with a larger genome highlights the fundamental features of Megaviridae
Information Genomique et Structurale, Centre National de la Recherche Scientifique Unité Propre de Recherche 2589, Aix Marseille University, Institut de Microbiologie de la Mediterranee, Parc Scientifique de Luminy, Case 934, FR 13288 Marseille, France
Proc Natl Acad Sci U S A 108:17486-91. 2011
- Nipah virus: a recently emergent deadly paramyxovirus
K B Chua
Department of Medical Microbiology, University of Malaya Medical Center, 50603 Kuala Lumpur, Malaysia
Science 288:1432-5. 2000
..Like Hendra virus, Nipah virus is unusual among the paramyxoviruses in its ability to infect and cause potentially fatal disease in a number of host species, including humans...
- A complicated message: Identification of a novel PB1-related protein translated from influenza A virus segment 2 mRNA
Helen M Wise
Department of Pathology, University of Cambridge, United Kingdom
J Virol 83:8021-31. 2009
..Thus, the influenza A virus proteome includes a 12th primary translation product that (similarly to PB1-F2) is nonessential for virus viability but whose loss, in particular genetic backgrounds, is detrimental to virus replication...
- A newly discovered human pneumovirus isolated from young children with respiratory tract disease
B G van den Hoogen
Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands
Nat Med 7:719-24. 2001
..Serological studies showed that by the age of five years, virtually all children in the Netherlands have been exposed to human metapneumovirus and that the virus has been circulating in humans for at least 50 years...
- Genetic detection and characterization of Lujo virus, a new hemorrhagic fever-associated arenavirus from southern Africa
Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, USA
PLoS Pathog 5:e1000455. 2009
..LUJV is a novel, genetically distinct, highly pathogenic arenavirus...
- Molecular mechanism of BST2/tetherin downregulation by K5/MIR2 of Kaposi's sarcoma-associated herpesvirus
Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, OR 97006, USA
J Virol 83:9672-81. 2009
..We further demonstrate that KSHV release is decreased in the absence of K5 in a BST2-dependent manner, suggesting that K5 contributes to the evasion of intracellular antiviral defense programs...
- Virus inhibition of RIP3-dependent necrosis
Jason W Upton
Department of Microbiology and Immunology, Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA 30322, USA
Cell Host Microbe 7:302-13. 2010
- A novel influenza A virus mitochondrial protein that induces cell death
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, USA
Nat Med 7:1306-12. 2001
..We propose that PB1-F2 functions to kill host immune cells responding to influenza virus infection...
- Expression of hepatitis C virus proteins induces distinct membrane alterations including a candidate viral replication complex
Institute for Medical Microbiology, University of Basel, CH 4003 Basel, Switzerland
J Virol 76:5974-84. 2002
..Virchows Arch. B., 33:233-243, 1980). In view of this finding and the observation that all HCV proteins accumulate on the membranous web, we propose that the membranous web forms the viral replication complex in HCV-infected cells...
- Herpesvirus assembly: an update
Thomas C Mettenleiter
Friedrich Loeffler Institut, 17493 Greifswald Insel Riems, Germany
Virus Res 143:222-34. 2009
..C., 2004. Budding events in herpesvirus morphogenesis. Virus Res. 106, 167-180]...
- The landscape of human proteins interacting with viruses and other pathogens
Matthew D Dyer
Genetics, Bioinformatics, and Computational Biology Program, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, United States of America
PLoS Pathog 4:e32. 2008
..Supplementary data accompanying this paper is available at http://staff.vbi.vt.edu/dyermd/publications/dyer2008a.html...
- The influenza virus protein PB1-F2 inhibits the induction of type I interferon at the level of the MAVS adaptor protein
Zsuzsanna T Varga
Department of Microbiology, Mount Sinai School of Medicine, New York City, New York, United States of America
PLoS Pathog 7:e1002067. 2011
..In summary, we have characterized the anti-interferon function of PB1-F2 and we suggest that this activity contributes to the enhanced pathogenicity seen with PB1-F2 N66S- expressing influenza viruses...
- The pathophysiology of mitochondrial cell death
Douglas R Green
Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, CA 92121, USA
Science 305:626-9. 2004
..The general rules governing the pathophysiology of MOMP and controversial issues regarding its regulation are discussed...
- HTLV-I basic leucine zipper factor gene mRNA supports proliferation of adult T cell leukemia cells
Laboratory of Virus Immunology, Institute for Virus Research, Kyoto University, 53 Shogoin Kawahara cho, Sakyo ku, Kyoto 606 8507, Japan
Proc Natl Acad Sci U S A 103:720-5. 2006
..Thus, the single HBZ gene has bimodal functions in two different molecular forms. The growth-promoting activity of HBZ RNA likely plays an important role in oncogenesis by HTLV-I...
- Viral latency and its regulation: lessons from the gamma-herpesviruses
Samuel H Speck
Emory Vaccine Center, Emory University, Atlanta, GA 30322, USA
Cell Host Microbe 8:100-15. 2010
..Here, we review the molecular basis of latency, with special attention to the gamma-herpesviruses, in which the understanding of this process is most advanced...
- Interleukin-10 and the interleukin-10 receptor
K W Moore
Department of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology Inc, Palo Alto, California 94304, USA
Annu Rev Immunol 19:683-765. 2001
..This review highlights findings that have advanced our understanding of IL-10 and its receptor, as well as its in vivo function in health and disease...
- Compensatory mutations in E1, p7, NS2, and NS3 enhance yields of cell culture-infectious intergenotypic chimeric hepatitis C virus
Center for Hepatitis Research, Institute for Human Infections and Immunity, The University of Texas Medical Branch at Galveston, 301 University Boulevard, Galveston, TX 77555 1019, USA
J Virol 81:629-38. 2007
..We conclude that interactions between NS2 and E1 and p7 as well as between NS2 and NS3 are essential for virus assembly and/or release and that each of these viral proteins plays an important role in this process.
- The epigenetic landscape of latent Kaposi sarcoma-associated herpesvirus genomes
Heinrich Pette Institute for Experimental Virology and Immunology, Hamburg, Germany
PLoS Pathog 6:e1000935. 2010
..Our findings suggest that the modification patterns identified here induce a poised state of repression during viral latency, which can be rapidly reversed once the lytic cycle is induced...
- The PB2 subunit of the influenza virus RNA polymerase affects virulence by interacting with the mitochondrial antiviral signaling protein and inhibiting expression of beta interferon
Katy M Graef
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 3203, USA
J Virol 84:8433-45. 2010
..Overall this study implicates the PB2 protein in the regulation of host antiviral innate immune pathways and suggests an important role for the mitochondrial association of the PB2 protein in determining virulence...
- Identification of amino acids in HA and PB2 critical for the transmission of H5N1 avian influenza viruses in a mammalian host
Animal Influenza Laboratory of the Ministry of Agriculture and National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People s Republic of China
PLoS Pathog 5:e1000709. 2009
..These amino acids changes in PB2 and HA could serve as important molecular markers for assessing the pandemic potential of H5N1 field isolates...
- Cell entry of hepatitis C virus requires a set of co-receptors that include the CD81 tetraspanin and the SR-B1 scavenger receptor
Laboratoire de Vectorologie Rétrovirale et Thérapie Génique, INSERM U412, Institut Fédératif de Recherche 128, Ecole Normale Superieure de Lyon, 46 allee d Italie, 69364 Lyon Cedex 07, France
J Biol Chem 278:41624-30. 2003
..Finally, by correlating expression of HCV receptors and infectivity, we suggest that, besides CD81 and SR-B1, additional hepatocyte-specific co-factor(s) are necessary for HCV entry...
- Analysis of the aphthovirus 2A/2B polyprotein 'cleavage' mechanism indicates not a proteolytic reaction, but a novel translational effect: a putative ribosomal 'skip'
M L Donnelly
Centre for Biomolecular Sciences, School of Biology, Biomolecular Sciences Building, University of St Andrews, North Haugh, St Andrews KY16 9ST, UK
J Gen Virol 82:1013-25. 2001
..This process produces a ribosomal 'skip' from one codon to the next without the formation of a peptide bond...
- Hepatitis C virus p7 and NS2 proteins are essential for production of infectious virus
Christopher T Jones
Laboratory of Virology and Infectious Disease, Center for the Study of Hepatitis C, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
J Virol 81:8374-83. 2007
..The protease domain of NS2 was required for infectivity, whereas its catalytic active site was dispensable. We conclude that p7 and NS2 function at an early stage of virion morphogenesis, prior to the assembly of infectious virus...
- Hepatitis C virus p7 protein is crucial for assembly and release of infectious virions
Department of Molecular Virology, University of Heidelberg, Heidelberg, Germany
PLoS Pathog 3:e103. 2007
..Although much has been learned about the function of individual viral proteins, the role of the HCV p7 protein in virus replication is not known...
- Novel structural protein in porcine reproductive and respiratory syndrome virus encoded by an alternative ORF5 present in all arteriviruses
Craig R Johnson
Department of Veterinary and Biomedical Sciences, University of Minnesota, St Paul, MN 55108, USA
J Gen Virol 92:1107-16. 2011
..Its discovery also provides a new potential target for immunological and pharmacological intervention in PRRS...
- MicroRNA-196 represses Bach1 protein and hepatitis C virus gene expression in human hepatoma cells expressing hepatitis C viral proteins
Liver Biliary Pancreatic Center and the Liver, Digestive Diseases, and Metabolism Laboratory, Carolinas Medical Center, Charlotte, NC 28232 2861, USA
Hepatology 51:1494-504. 2010
..Moreover, there was no detectable effect of mutant miR-196 on Bach1 3'-UTR-dependent luciferase activity...
- Hepatitis C virus infection protein network
B de Chassey
IMAP Team, Inserm Unit 851, Lyon, France
Mol Syst Biol 4:230. 2008
..CORE protein appeared as a major perturbator of this network. Focal adhesion was identified as a new function affected by HCV, mainly by NS3 and NS5A proteins...
- The 'cleavage' activities of foot-and-mouth disease virus 2A site-directed mutants and naturally occurring '2A-like' sequences
M L Donnelly
Centre for Biomolecular Sciences, School of Biology, Biomolecular Sciences Building, University of St Andrews, North Haugh, St Andrews KY16 9ST, UK
J Gen Virol 82:1027-41. 2001
- Structure of the Lassa virus nucleoprotein reveals a dsRNA-specific 3' to 5' exonuclease activity essential for immune suppression
Kathryn M Hastie
Departments of Immunology and Microbial Science and Molecular Biology, and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
Proc Natl Acad Sci U S A 108:2396-401. 2011
..Thus, the nucleoprotein is a viral exonuclease with anti-immune activity, and this work provides a unique opportunity to combat arenaviral infections...
- Viral evasion and subversion of pattern-recognition receptor signalling
Andrew G Bowie
School of Biochemistry and Immunology, Trinity College Dublin, Dublin 2, Ireland
Nat Rev Immunol 8:911-22. 2008
- Epstein-Barr virus: 40 years on
Lawrence S Young
Cancer Research UK Institute for Cancer Studies, University of Birmingham, Birmingham, B15 2TT, UK
Nat Rev Cancer 4:757-68. 2004
..Despite such ubiquity, the link between EBV and 'endemic' Burkitt's lymphoma proved consistent and became the first of an unexpectedly wide range of associations discovered between this virus and tumours...
- Differential inhibition of type I interferon induction by arenavirus nucleoproteins
Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029, USA
J Virol 81:12696-703. 2007
..Consistent with this observation, TCRV-infected cells also failed to inhibit interferon production...
- The structural basis for cap binding by influenza virus polymerase subunit PB2
Grenoble Outstation, European Molecular Biology Laboratory, 6 rue Jules Horowitz, BP181, 38042 Grenoble Cedex 9, France
Nat Struct Mol Biol 15:500-6. 2008
..These findings clarify the nature of the cap binding site in PB2 and will allow efficient structure-based design of new anti-influenza compounds inhibiting viral transcription...
- VirusMINT: a viral protein interaction database
Department of Biology, University of Rome Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy
Nucleic Acids Res 37:D669-73. 2009
..VirusMINT currently stores over 5000 interactions involving more than 490 unique viral proteins from more than 110 different viral strains...
- Chapare virus, a newly discovered arenavirus isolated from a fatal hemorrhagic fever case in Bolivia
Centro de Salud de Eterazama, Cochabamba, Bolivia
PLoS Pathog 4:e1000047. 2008
..In conclusion, two different arenaviruses, Machupo and Chapare, can be associated with severe HF cases in Bolivia...
- Cap binding and immune evasion revealed by Lassa nucleoprotein structure
Biomedical Sciences Research Complex, School of Chemistry, University of St Andrews, North Haugh, St Andrews, Fife KY16 9ST, UK
Nature 468:779-83. 2010
..These findings provide great potential for vaccine and drug development...
- DAI/ZBP1/DLM-1 complexes with RIP3 to mediate virus-induced programmed necrosis that is targeted by murine cytomegalovirus vIRA
Jason W Upton
Department of Microbiology and Immunology, Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA 30322, USA
Cell Host Microbe 11:290-7. 2012
..Thus, DAI interacts with RIP3 to mediate virus-induced necrosis analogous to the RIP1-RIP3 complex controlling death receptor-induced necroptosis. These studies unveil a role for DAI as the RIP3 partner mediating virus-induced necrosis...
- Failure to activate the IFN-β promoter by a paramyxovirus lacking an interferon antagonist
M J Killip
School of Biology, Centre for Biomolecular Sciences, North Haugh, University of St Andrews, St Andrews, Fife, UK
Virology 415:39-46. 2011
- High efficiency mutagenesis, repair, and engineering of chromosomal DNA using single-stranded oligonucleotides
H M Ellis
Gene Regulation and Chromosome Biology Laboratory, Division of Basic Sciences, National Cancer Institute at Frederick, P.O. Box B, Frederick, MD 21702, USA
Proc Natl Acad Sci U S A 98:6742-6. 2001
..Beta protein and ssDNA may prove generally applicable for repairing DNA in many organisms...
- The cap-snatching endonuclease of influenza virus polymerase resides in the PA subunit
Unit of Virus Host Cell Interactions, UJF EMBL CNRS, UMR 5233, 6 rue Jules Horowitz, BP181, 38042 Grenoble Cedex 9, France
Nature 458:914-8. 2009
..These results will facilitate the optimisation of endonuclease inhibitors as potential new anti-influenza drugs...
- 5'-Triphosphate RNA is the ligand for RIG-I
Division of Clinical Pharmacology, Department of Internal Medicine, University of Munich, 80336 Munich, Germany
Science 314:994-7. 2006
- Viral control of mitochondrial apoptosis
INSERM, U848, Villejuif, France
PLoS Pathog 4:e1000018. 2008
..Here, we summarize the current knowledge on the viral modulation of mitochondrial apoptosis, by focusing in particular on the mechanisms by which viral proteins control the host cell death apparatus.
- The interferon system and vaccinia virus evasion mechanisms
Department of Cellular and Molecular Biology, Centro Nacional de Biotecnologia, CSIC, Ciudad Universitaria Cantoblanco, Madrid, Spain
J Interferon Cytokine Res 29:581-98. 2009
..The molecular dissection of how VACV prevents the IFN response is providing important insights on our understanding of antiviral action and immune surveillance...
- Essential role of domain III of nonstructural protein 5A for hepatitis C virus infectious particle assembly
Department of Molecular Virology, University of Heidelberg, Heidelberg, Germany
PLoS Pathog 4:e1000035. 2008
..Since domain III of NS5A is one of the most variable regions in the HCV genome, the results suggest that viral isolates may differ in their level of virion production and thus in their level of fitness and pathogenesis...
- Host determinant residue lysine 627 lies on the surface of a discrete, folded domain of influenza virus polymerase PB2 subunit
Grenoble Outstation, European Molecular Biology Laboratory, Grenoble, France
PLoS Pathog 4:e1000136. 2008
..Host specificity is determined by multiple sites in different viral proteins, and mutation of only a limited number of these sites can lead to inter-species transmission...
- A concerted action of hepatitis C virus p7 and nonstructural protein 2 regulates core localization at the endoplasmic reticulum and virus assembly
Universite de Lyon, Lyon, France
PLoS Pathog 7:e1002144. 2011
..In conclusion, we identified p7 and NS2 as key determinants governing the subcellular localization of HCV core to LDs vs. ER and required for initiation of the early steps of virus assembly...
- Crystal structure of an avian influenza polymerase PA(N) reveals an endonuclease active site
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
Nature 458:909-13. 2009
..The high conservation of this endonuclease active site among influenza strains indicates that PA(N) is an important target for the design of new anti-influenza therapeutics...
- Hepatitis C virus protease NS3/4A cleaves mitochondrial antiviral signaling protein off the mitochondria to evade innate immunity
Xiao Dong Li
Howard Hughes Medical Institute, Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9148, USA
Proc Natl Acad Sci U S A 102:17717-22. 2005
- Transmission of influenza virus in a mammalian host is increased by PB2 amino acids 627K or 627E/701N
Department of Microbiology, Mount Sinai School of Medicine, New York, NY, USA
PLoS Pathog 5:e1000252. 2009
..Thus, our data show that PB2 amino acids 627 and 701 are determinants of mammalian inter-host transmission in diverse virus backgrounds...
- Hepatitis C virus NS2 protein serves as a scaffold for virus assembly by interacting with both structural and nonstructural proteins
Department of Microbiology and Immunology, The University of Texas Medical Branch at Galveston, 301 University Boulevard, Galveston, TX 77555 0610, USA
J Virol 85:86-97. 2011
..p7 may play an accessory role by regulating NS2 membrane topology, which is important for NS2-mediated protein interactions and therefore NS2 function...
- Expression of baculovirus P35 prevents cell death in Drosophila
B A Hay
Howard Hughes Medical Institute, University of California Berkeley 94720 3200
Development 120:2121-9. 1994
..Identification of molecules that interact biochemically or genetically with P35 in Drosophila should provide important insights into how cell death is regulated...
- Hierarchical action and inhibition of plant Dicer-like proteins in antiviral defense
Institut de Biologie Moleculaire des Plantes, CNRS Unité Propre de Recherche UPR 2357, 12, rue du Général Zimmer, 67084 Strasbourg Cedex, France
Science 313:68-71. 2006
..These findings define a molecular framework for studying antiviral silencing and defense in plants...
- Adaptation of pandemic H1N1 influenza viruses in mice
Natalia A Ilyushina
Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, Tennessee 38105 3678, USA
J Virol 84:8607-16. 2010
..These modulators of virulence, therefore, may have been the driving components of early evolution, which paved the way for novel 2009 viruses in mammals...
- A bacteriolytic agent that detects and kills Bacillus anthracis
Laboratory of Bacteriql Pathogenesis and Immunology, The Rockefeller University, New York, New York 10021, USA
Nature 418:884-9. 2002
..The lytic specificity of PlyG was also exploited as part of a rapid method for the identification of B. anthracis. We conclude that PlyG is a tool for the treatment and detection of B. anthracis...
- Identification of amino acid residues critical for the anti-interferon activity of the nucleoprotein of the prototypic arenavirus lymphocytic choriomeningitis virus
Department of Immunology, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, New York 10029, USA
J Virol 83:11330-40. 2009
..In addition, A549 cells infected with rLCMV/NP*(D382A), but not with WT LCMV, produced IFN and failed to rescue replication of the IFN-sensitive Newcastle disease virus...
- Different trajectories of parallel evolution during viral adaptation
H A Wichman
Department of Biological Sciences, University of Idaho, Moscow, ID 83844, USA
Science 285:422-4. 1999
..However, the order of these changes differed between replicates, and parallel substitutions did not reflect the changes with the largest beneficial effects or indicate a common trajectory of adaptation...
- Plant viral movement proteins: agents for cell-to-cell trafficking of viral genomes
William J Lucas
Section of Plant Biology, College of Biological Sciences, University of California, One Shields Ave, Davis, CA 95616, USA
Virology 344:169-84. 2006
- The complete genomes and proteomes of 27 Staphylococcus aureus bacteriophages
Targanta Therapeutics, 7170 Frederick Banting, Second Floor, Ville Saint Laurent, QC, Canada H4S 2A1
Proc Natl Acad Sci U S A 102:5174-9. 2005
..These phage genomes offer an exciting opportunity to discern molecular mechanisms of phage evolution and diversity...
- Interaction of polymerase subunit PB2 and NP with importin alpha1 is a determinant of host range of influenza A virus
Institute of Virology, Philipps University Marburg, Germany
PLoS Pathog 4:e11. 2008
..These findings demonstrate that adaptation of the viral polymerase to the nuclear import machinery plays an important role in interspecies transmission of influenza virus...
- A Begomovirus DNAbeta-encoded protein binds DNA, functions as a suppressor of RNA silencing, and targets the cell nucleus
Institute of Biotechnology, Zhejiang University, Hangzhou 310029, People s Republic of China
J Virol 79:10764-75. 2005
- Genetic content of wild-type human cytomegalovirus
MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK
J Gen Virol 85:1301-12. 2004
..The case in support of a lack of protein-coding potential in the region between UL105 and UL111A was also strengthened...
- High-throughput identification of compounds targeting influenza RNA-dependent RNA polymerase activity
Ching Yao Su
Genomics Research Center, Academia Sinica, Taipei
Proc Natl Acad Sci U S A 107:19151-6. 2010
..Our high-throughput screening (HTS) campaign thus resulted in the identification of antiinfluenza compounds targeting RdRP activity...
- Genomic and proteomic characterization of the broad-host-range Salmonella phage PVP-SE1: creation of a new phage genus
Silvio B Santos
Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4700 057 Braga, Portugal
J Virol 85:11265-73. 2011
..Both phages are unrelated to any other known virus, which suggests that an "rV5-like virus" genus should be created within the Myoviridae to contain these two phages...
- Pulmonary bacteriophage therapy on Pseudomonas aeruginosa cystic fibrosis strains: first steps towards treatment and prevention
Molecular Biology of the Gene in Extremophiles Unit, Department of Microbiology, Institut Pasteur, Paris, France
PLoS ONE 6:e16963. 2011
..aeruginosa cystic fibrosis strains. This work provides an incentive to develop clinical studies on pulmonary bacteriophage therapy to combat multidrug-resistant lung infections...
- The virophage as a unique parasite of the giant mimivirus
Bernard La Scola
URMITE, Centre National de la Recherche Scientifique UMR IRD 6236, Faculte de Medecine, Universite de la Mediterranee, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 5, France
Nature 455:100-4. 2008
..Considering its functional analogy with bacteriophages, we classify this virus as a virophage. The virophage could be a vehicle mediating lateral gene transfer between giant viruses...
- Endoproteolytic processing of the lymphocytic choriomeningitis virus glycoprotein by the subtilase SKI-1/S1P
Winfried R Beyer
Heinrich Pette Institut fur experimentelle Virologie und Immunologie an der Universitat Hamburg, D 20251 Hamburg, Germany
J Virol 77:2866-72. 2003
..In agreement with the identified consensus motif, we show that the cellular subtilase SKI-1/S1P cleaves LCMV GP...
- The structure of a biologically active influenza virus ribonucleoprotein complex
Centro Nacional de Biotecnologia, Campus de Cantoblanco, Madrid, Spain
PLoS Pathog 5:e1000491. 2009
..The structure reported here will serve as a framework to generate a quasi-atomic model of the molecular machine responsible for viral RNA synthesis and to test new models for virus RNA replication and transcription...
- Classification of Myoviridae bacteriophages using protein sequence similarity
Biosystems Department, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, Leuven, B 3001, Belgium
BMC Microbiol 9:224. 2009
..It appears that horizontal gene transfer generally does not totally obliterate evolutionary relationships between phages...
- PB2 residue 158 is a pathogenic determinant of pandemic H1N1 and H5 influenza a viruses in mice
Wadsworth Center, NYSDOH, Griffin Laboratory, 5668 State Farm Road, Slingerlands, NY 12159, USA
J Virol 85:357-65. 2011
..Therefore, future surveillance efforts should include scrutiny of this region of PB2 because of its potential impact on pathogenesis...
- Synergistic adaptive mutations in the hemagglutinin and polymerase acidic protein lead to increased virulence of pandemic 2009 H1N1 influenza A virus in mice
Institute of Molecular Virology, ZMBE, Muenster, Germany
J Infect Dis 205:262-71. 2012
..In conclusion, synergistic action of all 3 mutations results in a mice lethal pandemic H1N1 virus...
- Single-stranded heteroduplex intermediates in lambda Red homologous recombination
Technische Universitat Dresden, BioInnovationsZentrum, Dresden, Germany
BMC Mol Biol 11:54. 2010
..The Red proteins of lambda phage mediate probably the simplest and most efficient homologous recombination reactions yet described. However the mechanism of dsDNA recombination remains undefined...
- HIV-specific CD4 T cell responses to different viral proteins have discordant associations with viral load and clinical outcome
Ragon Institute of MGH, MIT, and Harvard, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA
J Virol 86:277-83. 2012
..60 to 0.85). Inclusion of these immunogenic proteins and peptides in future HIV vaccines may act as a critical cornerstone for enhancing protective T cell responses...
- A conserved acetyl esterase domain targets diverse bacteriophages to the Vi capsular receptor of Salmonella enterica serovar Typhi
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Sulston Building, Hinxton, Cambridge CB10 1SA, United Kingdom
J Bacteriol 192:5746-54. 2010
..Thus, we provide a simple explanation and paradigm of how a diverse group of phages target a single key virulence antigen associated with this important human-restricted pathogen...
- Expression of the 1918 influenza A virus PB1-F2 enhances the pathogenesis of viral and secondary bacterial pneumonia
Julie L McAuley
Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, TN 38105, USA
Cell Host Microbe 2:240-9. 2007
..These findings help explain both the unparalleled virulence of the 1918 strain and the high incidence of fatal pneumonia during the pandemic...
- EBV microRNAs in primary lymphomas and targeting of CXCL-11 by ebv-mir-BHRF1-3
The Viral Oncology Program, Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL 33136, USA
Cancer Res 68:1436-42. 2008
..Targeted suppression of CXCL-11/I-TAC by a viral-encoded miRNA may serve as an immunomodulatory mechanism in these tumors...
- Evidence for avian and human host cell factors that affect the activity of influenza virus polymerase
Department of Virology, Division of Infectious Diseases, Imperial College London, Wright Fleming Institute, Norfolk Place, W2 1PG London, UK
J Virol 84:9978-86. 2010
..These four viral proteins constitute the minimal set for transcription and replication of influenza viral RNA...
- Trans-complementation of an NS2 defect in a late step in hepatitis C virus (HCV) particle assembly and maturation
Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, TX, USA
PLoS Pathog 5:e1000403. 2009
..systems that recapitulate the entire life cycle of hepatitis C virus (HCV) indicate that several nonstructural viral proteins, including NS2, NS3, and NS5A, are involved in the process of viral assembly and release...
- A system for functional analysis of Ebola virus glycoprotein
Department of Virology and Molecular Biology, St Jude Children s Research Hospital, 332 North Lauderdale, P O Box 318, Memphis, TN 38101, USA
Proc Natl Acad Sci U S A 94:14764-9. 1997
..Thus, our VSV system should be useful for investigating the functions of glycoproteins from highly pathogenic viruses or those incapable of being cultured in vitro...
- Nuclear import of cytoplasmic poly(A) binding protein restricts gene expression via hyperadenylation and nuclear retention of mRNA
G Renuka Kumar
Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720 3102, USA
Mol Cell Biol 30:4996-5008. 2010
- Intracellular proton conductance of the hepatitis C virus p7 protein and its contribution to infectious virus production
Ann L Wozniak
Department of Internal Medicine, University of Kansas Medical Center, Kansas City, Kansas, USA
PLoS Pathog 6:e1001087. 2010
..This loss of acidification is required for productive HCV infection, possibly through protecting nascent virus particles during an as yet uncharacterized maturation process...
- Conditional control of gene expression in the mouse
Section of Genetics of Vertebrate Development, Laboratory of Cancer and Developmental Biology, National Cancer Institute Frederick Cancer Research and Development Center, Frederick, Maryland 21702 1201, USA
Nat Rev Genet 2:743-55. 2001
..Here, I review these systems and discuss certain studies that exemplify the advantages and limitations of each system...
- Mobile DNA elements in T4 and related phages
David R Edgell
Department of Biochemistry, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, ON N6A5C1, Canada
Virol J 7:290. 2010
..We also discuss recent progress in the biology of free-standing endonucleases, and present areas of future research for this fascinating class of mobile genetic elements...
- The small RING finger protein Z drives arenavirus budding: implications for antiviral strategies
Department of Neuropharmacology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Proc Natl Acad Sci U S A 100:12978-83. 2003
..Moreover, members of the vacuolar protein sorting pathway appear to play an important role in arena-virus budding. These findings open possibilities for antiviral strategies to combat LFV and other hemorrhagic fever arenaviruses...
- The autophagy machinery is required to initiate hepatitis C virus replication
Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA
Proc Natl Acad Sci U S A 106:14046-51. 2009
- Functional analysis of recombinant respiratory syncytial virus deletion mutants lacking the small hydrophobic and/or attachment glycoprotein gene
Department of Immunology/Microbiology, Rush-Presbyterian-St. Luke's Medical Center, Chicago, Illinois 60612, USA
J Virol 75:6825-34. 2001
- Molecular basis for high virulence of Hong Kong H5N1 influenza A viruses
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, 2015 Linden Drive, Madison, WI 53706, USA
Science 293:1840-2. 2001
..Moreover, high cleavability of the hemagglutinin glycoprotein was an essential requirement for lethal infection...
- Multiple amino acid substitutions are involved in the adaptation of H9N2 avian influenza virus to mice
Institute of Animal Husbandry and Veterinary Science, Hubei Academy of Agricultural Sciences, Wuhan 430064, Hubei, China
Vet Microbiol 138:85-91. 2009
..We conclude that multiple amino acid substitutions are likely to be involved in the adaptation of H9N2 avian influenza virus to mice...
- Nuclear factor 90 negatively regulates influenza virus replication by interacting with viral nucleoprotein
Department of Microbiology, The University of Hong Kong, Pokfulam, People s Republic of China
J Virol 83:7850-61. 2009
..These results suggest that NF90 inhibits influenza virus replication during the early phase of infection through direct interaction with viral NP...
- The DNA beta satellite component associated with ageratum yellow vein disease encodes an essential pathogenicity protein (betaC1)
Department of Disease and Stress Biology, John Innes Centre, Colney, Norwich NR4 7UH, UK
Virology 324:37-47. 2004
..Our results demonstrate that the satellite encodes at least one protein that plays a major role in symptom development and is essential for disease progression in ageratum, the natural host of the AYVD complex...
- Identification of proteins in human cytomegalovirus (HCMV) particles: the HCMV proteome
Susan M Varnum
Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland, OR 97201, USA
J Virol 78:10960-6. 2004
..proteins from purified HCMV virion preparations has indicated that the particle contains significantly more viral proteins than previously known...
- Viral targeting of DEAD box protein 3 reveals its role in TBK1/IKKepsilon-mediated IRF activation
Viral Immune Evasion Group, School of Biochemistry and Immunology, Trinity College Dublin, Dublin, Ireland
EMBO J 27:2147-57. 2008
..Therefore, this study shows for the first time the involvement of a DEAD box helicase in TBK1/IKKepsilon-mediated IRF activation and Ifnb promoter induction...
- Influenza A virus polymerase inhibits type I interferon induction by binding to interferon beta promoter stimulator 1
Department of Bioresources, hHokkaido University Research Center for Zoonosis Control, North 20, West 10 Kita ku, Sapporo, Hokkaido 001 0020, Japan
J Biol Chem 285:32064-74. 2010
..These findings demonstrate that the viral polymerase plays an important role for regulating host anti-viral response through the binding to IPS-1 and inhibition of IFNβ production...
- Patterns of HIV-1 protein interaction identify perturbed host-cellular subsystems
Jamie I Macpherson
Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom
PLoS Comput Biol 6:e1000863. 2010
..Thus, our work disentangles the complex set of HIV-1-host protein interactions in the HHPID, reconciles these with siRNA screens and provides an accessible and interpretable map of infection...
- Is HCMV a tumor promoter?
California Pacific Medical Center Research Institute, 475 Brannan Street, Suite 220, San Francisco, CA 94107, USA liliana
Virus Res 157:193-203. 2011
..This review highlights some of the recent evidence that HCMV might play a role in modulating the tumor microenvironment as well as in the initiation and promotion of tumor cells themselves...
- Phages will out: strategies of host cell lysis
Dept of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77843 2128, USA
Trends Microbiol 8:120-8. 2000
..By contrast, lytic single-stranded RNA and DNA phages accomplish lysis by producing a single lysis protein without muralytic activity...
- Hepatitis C virus NS2 protein contributes to virus particle assembly via opposing epistatic interactions with the E1-E2 glycoprotein and NS3-NS4A enzyme complexes
Section of Microbial Pathogenesis, Yale University School of Medicine, 295 Congress Ave, New Haven, CT 06536, USA
J Virol 83:8379-95. 2009
..These data reveal a complex network of interactions involving NS2 and other viral structural and nonstructural proteins during virus assembly...