- Generalized lacZ expression with the ROSA26 Cre reporter strain
Nat Genet 21:70-1. 1999
- The multifunctional NS1 protein of influenza A viruses
Benjamin G Hale
Centre for Biomolecular Sciences, University of St Andrews, St Andrews, Fife KY16 9ST, UK
J Gen Virol 89:2359-76. 2008
- A physical and regulatory map of host-influenza interactions reveals pathways in H1N1 infection
Sagi D Shapira
Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA
Cell 139:1255-67. 2009
During the course of a viral infection, viral proteins interact with an array of host proteins and pathways. Here, we present a systematic strategy to elucidate the dynamic interactions between H1N1 influenza and its human host...
- Replication of hepatitis C virus
Division of Gastroenterology and Hepatology, Centre Hospitalier Universitaire Vaudois, University of Lausanne, CH 1011 Lausanne, Switzerland
Nat Rev Microbiol 5:453-63. 2007
..These efforts have already translated into the identification of novel antiviral targets and the development of new therapeutic strategies, some of which are currently undergoing clinical evaluation...
- An efficient recombination system for chromosome engineering in Escherichia coli
Gene Regulation and Chromosome Biology Laboratory and Mouse Cancer Genetics Program, National Cancer Institute, Division of Basic Science, National Cancer Institute Frederick Cancer Research and Development Center, Frederick, MD 21702, USA
Proc Natl Acad Sci U S A 97:5978-83. 2000
..This system will be especially useful for the engineering of large bacterial plasmids such as those from bacterial artificial chromosome libraries...
- A novel influenza A virus mitochondrial protein that induces cell death
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, USA
Nat Med 7:1306-12. 2001
..We propose that PB1-F2 functions to kill host immune cells responding to influenza virus infection...
- The cap-snatching endonuclease of influenza virus polymerase resides in the PA subunit
Unit of Virus Host Cell Interactions, UJF EMBL CNRS, UMR 5233, 6 rue Jules Horowitz, BP181, 38042 Grenoble Cedex 9, France
Nature 458:914-8. 2009
..These results will facilitate the optimisation of endonuclease inhibitors as potential new anti-influenza drugs...
- Analysis of the aphthovirus 2A/2B polyprotein 'cleavage' mechanism indicates not a proteolytic reaction, but a novel translational effect: a putative ribosomal 'skip'
M L Donnelly
Centre for Biomolecular Sciences, School of Biology, Biomolecular Sciences Building, University of St Andrews, North Haugh, St Andrews KY16 9ST, UK
J Gen Virol 82:1013-25. 2001
..This process produces a ribosomal 'skip' from one codon to the next without the formation of a peptide bond...
- A newly discovered human pneumovirus isolated from young children with respiratory tract disease
B G van den Hoogen
Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands
Nat Med 7:719-24. 2001
..Serological studies showed that by the age of five years, virtually all children in the Netherlands have been exposed to human metapneumovirus and that the virus has been circulating in humans for at least 50 years...
- Molecular mechanism of BST2/tetherin downregulation by K5/MIR2 of Kaposi's sarcoma-associated herpesvirus
Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, OR 97006, USA
J Virol 83:9672-81. 2009
..We further demonstrate that KSHV release is decreased in the absence of K5 in a BST2-dependent manner, suggesting that K5 contributes to the evasion of intracellular antiviral defense programs...
- Genetic detection and characterization of Lujo virus, a new hemorrhagic fever-associated arenavirus from southern Africa
Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY, USA
PLoS Pathog 5:e1000455. 2009
..LUJV is a novel, genetically distinct, highly pathogenic arenavirus...
- Fusing structure and function: a structural view of the herpesvirus entry machinery
Sarah A Connolly
Department of Microbiology and Immunology, The Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA
Nat Rev Microbiol 9:369-81. 2011
- Cucumber mosaic virus-encoded 2b suppressor inhibits Arabidopsis Argonaute1 cleavage activity to counter plant defense
Laboratory of Plant Molecular Biology, Rockefeller University, New York, New York 10021, USA
Genes Dev 20:3255-68. 2006
..These findings provide insight on the molecular arms race between host antiviral RNA silencing and virus counterdefense...
- Hierarchical action and inhibition of plant Dicer-like proteins in antiviral defense
Institut de Biologie Moleculaire des Plantes, CNRS Unité Propre de Recherche UPR 2357, 12, rue du Général Zimmer, 67084 Strasbourg Cedex, France
Science 313:68-71. 2006
..These findings define a molecular framework for studying antiviral silencing and defense in plants...
- Virus inhibition of RIP3-dependent necrosis
Jason W Upton
Department of Microbiology and Immunology, Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA 30322, USA
Cell Host Microbe 7:302-13. 2010
- 5'-Triphosphate RNA is the ligand for RIG-I
Division of Clinical Pharmacology, Department of Internal Medicine, University of Munich, 80336 Munich, Germany
Science 314:994-7. 2006
- Metagenomic analyses of an uncultured viral community from human feces
Department of Biology, San Diego State University, San Diego, California 92182, USA
J Bacteriol 185:6220-3. 2003
..Most of the sequences were unrelated to anything previously reported. The recognizable viruses were mostly siphophages, and the community contained an estimated 1,200 viral genotypes...
- Crystal structure of an avian influenza polymerase PA(N) reveals an endonuclease active site
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
Nature 458:909-13. 2009
..The high conservation of this endonuclease active site among influenza strains indicates that PA(N) is an important target for the design of new anti-influenza therapeutics...
- Herpesvirus assembly: an update
Thomas C Mettenleiter
Friedrich Loeffler Institut, 17493 Greifswald Insel Riems, Germany
Virus Res 143:222-34. 2009
..C., 2004. Budding events in herpesvirus morphogenesis. Virus Res. 106, 167-180]...
- The landscape of human proteins interacting with viruses and other pathogens
Matthew D Dyer
Genetics, Bioinformatics, and Computational Biology Program, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, United States of America
PLoS Pathog 4:e32. 2008
..Supplementary data accompanying this paper is available at http://staff.vbi.vt.edu/dyermd/publications/dyer2008a.html...
- The 'cleavage' activities of foot-and-mouth disease virus 2A site-directed mutants and naturally occurring '2A-like' sequences
M L Donnelly
Centre for Biomolecular Sciences, School of Biology, Biomolecular Sciences Building, University of St Andrews, North Haugh, St Andrews KY16 9ST, UK
J Gen Virol 82:1027-41. 2001
- Phylogeny of the genus Flavivirus
Division of Vector Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522 2087, USA
J Virol 72:73-83. 1998
..We also resolved many of the past taxonomic problems by establishing phylogenetic relationships of the antigenically unclassified viruses with the well-established viruses and by identifying synonymous viruses...
- Genetic content of wild-type human cytomegalovirus
MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK
J Gen Virol 85:1301-12. 2004
..The case in support of a lack of protein-coding potential in the region between UL105 and UL111A was also strengthened...
- Subregion- and cell type-restricted gene knockout in mouse brain
J Z Tsien
Howard Hughes Medical Institute, Center for Learning and Memory, Massachusetts Institute of Technology, Cambridge 02139, USA
Cell 87:1317-26. 1996
..The brain subregional restricted gene knockout should allow a more precise analysis of the impact of a gene mutation on animal behaviors...
- The pathophysiology of mitochondrial cell death
Douglas R Green
Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, CA 92121, USA
Science 305:626-9. 2004
..The general rules governing the pathophysiology of MOMP and controversial issues regarding its regulation are discussed...
- HTLV-I basic leucine zipper factor gene mRNA supports proliferation of adult T cell leukemia cells
Laboratory of Virus Immunology, Institute for Virus Research, Kyoto University, 53 Shogoin Kawahara cho, Sakyo ku, Kyoto 606 8507, Japan
Proc Natl Acad Sci U S A 103:720-5. 2006
..Thus, the single HBZ gene has bimodal functions in two different molecular forms. The growth-promoting activity of HBZ RNA likely plays an important role in oncogenesis by HTLV-I...
- Viral latency and its regulation: lessons from the gamma-herpesviruses
Samuel H Speck
Emory Vaccine Center, Emory University, Atlanta, GA 30322, USA
Cell Host Microbe 8:100-15. 2010
..Here, we review the molecular basis of latency, with special attention to the gamma-herpesviruses, in which the understanding of this process is most advanced...
- Interleukin-10 and the interleukin-10 receptor
K W Moore
Department of Molecular Biology, DNAX Research Institute of Molecular and Cellular Biology Inc, Palo Alto, California 94304, USA
Annu Rev Immunol 19:683-765. 2001
..This review highlights findings that have advanced our understanding of IL-10 and its receptor, as well as its in vivo function in health and disease...
- High-resolution human cytomegalovirus transcriptome
Medical Research Council University of Glasgow Centre for Virus Research, Glasgow G11 5JR, United Kingdom
Proc Natl Acad Sci U S A 108:19755-60. 2011
..The study also illustrates the key contribution that deep sequencing can make to the genomics of nuclear DNA viruses...
- Avian-to-human transmission of the PB1 gene of influenza A viruses in the 1957 and 1968 pandemics
Department of Virology Molecular Biology, St Jude Children s Research Hospital, Memphis, Tennessee 38101 0318
J Virol 63:4603-8. 1989
..Comparative analysis of deduced amino acid sequences disclosed highly conserved regions in PB1 proteins, which may be key structures required for PB1 activities...
- A genome-wide genetic screen for host factors required for hepatitis C virus propagation
Liver Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 106:16410-5. 2009
..The identification of host factors participating in the complete HCV lifecycle will both advance our understanding of HCV pathogenesis and illuminate therapeutic targets...
- Compensatory mutations in E1, p7, NS2, and NS3 enhance yields of cell culture-infectious intergenotypic chimeric hepatitis C virus
Center for Hepatitis Research, Institute for Human Infections and Immunity, The University of Texas Medical Branch at Galveston, 301 University Boulevard, Galveston, TX 77555 1019, USA
J Virol 81:629-38. 2007
..We conclude that interactions between NS2 and E1 and p7 as well as between NS2 and NS3 are essential for virus assembly and/or release and that each of these viral proteins plays an important role in this process.
- The PB2 subunit of the influenza virus RNA polymerase affects virulence by interacting with the mitochondrial antiviral signaling protein and inhibiting expression of beta interferon
Katy M Graef
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 3203, USA
J Virol 84:8433-45. 2010
..Overall this study implicates the PB2 protein in the regulation of host antiviral innate immune pathways and suggests an important role for the mitochondrial association of the PB2 protein in determining virulence...
- Identification of amino acids in HA and PB2 critical for the transmission of H5N1 avian influenza viruses in a mammalian host
Animal Influenza Laboratory of the Ministry of Agriculture and National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People s Republic of China
PLoS Pathog 5:e1000709. 2009
..These amino acids changes in PB2 and HA could serve as important molecular markers for assessing the pandemic potential of H5N1 field isolates...
- Cell entry of hepatitis C virus requires a set of co-receptors that include the CD81 tetraspanin and the SR-B1 scavenger receptor
Laboratoire de Vectorologie Rétrovirale et Thérapie Génique, INSERM U412, Institut Fédératif de Recherche 128, Ecole Normale Superieure de Lyon, 46 allee d Italie, 69364 Lyon Cedex 07, France
J Biol Chem 278:41624-30. 2003
..Finally, by correlating expression of HCV receptors and infectivity, we suggest that, besides CD81 and SR-B1, additional hepatocyte-specific co-factor(s) are necessary for HCV entry...
- Hepatitis C virus p7 and NS2 proteins are essential for production of infectious virus
Christopher T Jones
Laboratory of Virology and Infectious Disease, Center for the Study of Hepatitis C, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
J Virol 81:8374-83. 2007
..The protease domain of NS2 was required for infectivity, whereas its catalytic active site was dispensable. We conclude that p7 and NS2 function at an early stage of virion morphogenesis, prior to the assembly of infectious virus...
- Hepatitis C virus p7 protein is crucial for assembly and release of infectious virions
Department of Molecular Virology, University of Heidelberg, Heidelberg, Germany
PLoS Pathog 3:e103. 2007
..Although much has been learned about the function of individual viral proteins, the role of the HCV p7 protein in virus replication is not known...
- Distant Mimivirus relative with a larger genome highlights the fundamental features of Megaviridae
Information Genomique et Structurale, Centre National de la Recherche Scientifique Unité Propre de Recherche 2589, Aix Marseille University, Institut de Microbiologie de la Mediterranee, Parc Scientifique de Luminy, Case 934, FR 13288 Marseille, France
Proc Natl Acad Sci U S A 108:17486-91. 2011
- A complicated message: Identification of a novel PB1-related protein translated from influenza A virus segment 2 mRNA
Helen M Wise
Department of Pathology, University of Cambridge, United Kingdom
J Virol 83:8021-31. 2009
..Thus, the influenza A virus proteome includes a 12th primary translation product that (similarly to PB1-F2) is nonessential for virus viability but whose loss, in particular genetic backgrounds, is detrimental to virus replication...
- Nipah virus: a recently emergent deadly paramyxovirus
K B Chua
Department of Medical Microbiology, University of Malaya Medical Center, 50603 Kuala Lumpur, Malaysia
Science 288:1432-5. 2000
..Like Hendra virus, Nipah virus is unusual among the paramyxoviruses in its ability to infect and cause potentially fatal disease in a number of host species, including humans...
- Vaccinia virus protein C6 is a virulence factor that binds TBK-1 adaptor proteins and inhibits activation of IRF3 and IRF7
School of Biochemistry and Immunology, Trinity College Dublin, Dublin, Ireland
PLoS Pathog 7:e1002247. 2011
..Thus C6 contributes to VACV virulence and might do so via the inhibition of PRR-induced activation of IRF3 and IRF7...
- Epstein-Barr virus: 40 years on
Lawrence S Young
Cancer Research UK Institute for Cancer Studies, University of Birmingham, Birmingham, B15 2TT, UK
Nat Rev Cancer 4:757-68. 2004
..Despite such ubiquity, the link between EBV and 'endemic' Burkitt's lymphoma proved consistent and became the first of an unexpectedly wide range of associations discovered between this virus and tumours...
- Hepatitis C virus infection protein network
B de Chassey
IMAP Team, Inserm Unit 851, Lyon, France
Mol Syst Biol 4:230. 2008
..CORE protein appeared as a major perturbator of this network. Focal adhesion was identified as a new function affected by HCV, mainly by NS3 and NS5A proteins...
- Structure of the Lassa virus nucleoprotein reveals a dsRNA-specific 3' to 5' exonuclease activity essential for immune suppression
Kathryn M Hastie
Departments of Immunology and Microbial Science and Molecular Biology, and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
Proc Natl Acad Sci U S A 108:2396-401. 2011
..Thus, the nucleoprotein is a viral exonuclease with anti-immune activity, and this work provides a unique opportunity to combat arenaviral infections...
- MicroRNA-196 represses Bach1 protein and hepatitis C virus gene expression in human hepatoma cells expressing hepatitis C viral proteins
Liver Biliary Pancreatic Center and the Liver, Digestive Diseases, and Metabolism Laboratory, Carolinas Medical Center, Charlotte, NC 28232 2861, USA
Hepatology 51:1494-504. 2010
..Moreover, there was no detectable effect of mutant miR-196 on Bach1 3'-UTR-dependent luciferase activity...
- Viral evasion and subversion of pattern-recognition receptor signalling
Andrew G Bowie
School of Biochemistry and Immunology, Trinity College Dublin, Dublin 2, Ireland
Nat Rev Immunol 8:911-22. 2008
- Growth inhibition and DNA damage induced by Cre recombinase in mammalian cells
Division of Molecular Genetics and Center of Biomedical Genetics, Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands
Proc Natl Acad Sci U S A 98:9209-14. 2001
..Prolonged, low levels of Cre activity permit recombination without concomitant toxicity. This urges for a careful titration of Cre activity in conditional gene modification in mammalian cells...
- Expression of hepatitis C virus proteins induces distinct membrane alterations including a candidate viral replication complex
Institute for Medical Microbiology, University of Basel, CH 4003 Basel, Switzerland
J Virol 76:5974-84. 2002
..Virchows Arch. B., 33:233-243, 1980). In view of this finding and the observation that all HCV proteins accumulate on the membranous web, we propose that the membranous web forms the viral replication complex in HCV-infected cells...
- Inhibition of interferon signaling by dengue virus
Jorge L Munoz-Jordan
Department of Microbiology, Mount Sinai School of Medicine, One Gustave Levy Place, New York, NY 10029
Proc Natl Acad Sci U S A 100:14333-8. 2003
..This protein, encoded by a positive strand RNA virus, is implicated as an IFN-signaling inhibitor...
- Differential inhibition of type I interferon induction by arenavirus nucleoproteins
Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029, USA
J Virol 81:12696-703. 2007
..Consistent with this observation, TCRV-infected cells also failed to inhibit interferon production...
- The structural basis for cap binding by influenza virus polymerase subunit PB2
Grenoble Outstation, European Molecular Biology Laboratory, 6 rue Jules Horowitz, BP181, 38042 Grenoble Cedex 9, France
Nat Struct Mol Biol 15:500-6. 2008
..These findings clarify the nature of the cap binding site in PB2 and will allow efficient structure-based design of new anti-influenza compounds inhibiting viral transcription...
- Novel structural protein in porcine reproductive and respiratory syndrome virus encoded by an alternative ORF5 present in all arteriviruses
Craig R Johnson
Department of Veterinary and Biomedical Sciences, University of Minnesota, St Paul, MN 55108, USA
J Gen Virol 92:1107-16. 2011
..Its discovery also provides a new potential target for immunological and pharmacological intervention in PRRS...
- The protein network of HIV budding
Uta K von Schwedler
Department of Biochemistry, University of Utah, Salt Lake City, UT 84132, USA
Cell 114:701-13. 2003
..These studies define a protein network required for human MVB biogenesis and indicate that the entire network participates in the release of HIV and probably many other viruses...
- Common origin of four diverse families of large eukaryotic DNA viruses
L M Iyer
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894, USA
J Virol 75:11720-34. 2001
- Cap binding and immune evasion revealed by Lassa nucleoprotein structure
Biomedical Sciences Research Complex, School of Chemistry, University of St Andrews, North Haugh, St Andrews, Fife KY16 9ST, UK
Nature 468:779-83. 2010
..These findings provide great potential for vaccine and drug development...
- Chapare virus, a newly discovered arenavirus isolated from a fatal hemorrhagic fever case in Bolivia
Centro de Salud de Eterazama, Cochabamba, Bolivia
PLoS Pathog 4:e1000047. 2008
..In conclusion, two different arenaviruses, Machupo and Chapare, can be associated with severe HF cases in Bolivia...
- Latency-associated degradation of the MRP1 drug transporter during latent human cytomegalovirus infection
Michael P Weekes
Cambridge Institute for Medical Research, University of Cambridge, Cambridge, UK
Science 340:199-202. 2013
..The UL138-mediated loss of MRP1 provides a marker for detecting latent HCMV infection and a therapeutic target for eliminating latently infected cells before transplantation...
- Influenza virus propagation is impaired by inhibition of the Raf/MEK/ERK signalling cascade
, , , D-35392 Giessen, Germany
Nat Cell Biol 3:301-5. 2001
..The functions of most of the ten different viral proteins of this negative-strand RNA virus have been well elucidated...
- Small RNA binding is a common strategy to suppress RNA silencing by several viral suppressors
Agricultural Biotechnology Center, Plant Biology Institute, Godollo, Hungary
EMBO J 25:2768-80. 2006
..None of the suppressors affected the activity of preassembled RISC complexes. In contrast, each suppressor uniformly inhibited the siRNA-initiated RISC assembly pathway by preventing RNA silencing initiator complex formation...
- Concurrent expression of latent and a limited number of lytic genes with immune modulation and antiapoptotic function by Kaposi's sarcoma-associated herpesvirus early during infection of primary endothelial and fibroblast cells and subsequent decline of l
Harinivas H Krishnan
Department of Microbiology, Molecular Genetics and Immunology, The University of Kansas Medical Center, Kansas City, Kansas, USA
J Virol 78:3601-20. 2004
..These are unique observations among in vitro herpesvirus infections and may have important implications in KSHV biology and pathogenesis...
- Identification of virus-encoded microRNAs
Laboratory of RNA Molecular Biology, The Rockefeller University, 1230 York Avenue, Box 186, New York, NY 10021, USA
Science 304:734-6. 2004
..Given that miRNAs function in RNA silencing pathways either by targeting messenger RNAs for degradation or by repressing translation, we identified viral regulators of host and/or viral gene expression...
- Viral targeting of DEAD box protein 3 reveals its role in TBK1/IKKepsilon-mediated IRF activation
Viral Immune Evasion Group, School of Biochemistry and Immunology, Trinity College Dublin, Dublin, Ireland
EMBO J 27:2147-57. 2008
..Therefore, this study shows for the first time the involvement of a DEAD box helicase in TBK1/IKKepsilon-mediated IRF activation and Ifnb promoter induction...
- DAI/ZBP1/DLM-1 complexes with RIP3 to mediate virus-induced programmed necrosis that is targeted by murine cytomegalovirus vIRA
Jason W Upton
Department of Microbiology and Immunology, Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA 30322, USA
Cell Host Microbe 11:290-7. 2012
..Thus, DAI interacts with RIP3 to mediate virus-induced necrosis analogous to the RIP1-RIP3 complex controlling death receptor-induced necroptosis. These studies unveil a role for DAI as the RIP3 partner mediating virus-induced necrosis...
- Failure to activate the IFN-β promoter by a paramyxovirus lacking an interferon antagonist
M J Killip
School of Biology, Centre for Biomolecular Sciences, North Haugh, University of St Andrews, St Andrews, Fife, UK
Virology 415:39-46. 2011
- High efficiency mutagenesis, repair, and engineering of chromosomal DNA using single-stranded oligonucleotides
H M Ellis
Gene Regulation and Chromosome Biology Laboratory, Division of Basic Sciences, National Cancer Institute at Frederick, P.O. Box B, Frederick, MD 21702, USA
Proc Natl Acad Sci U S A 98:6742-6. 2001
..Beta protein and ssDNA may prove generally applicable for repairing DNA in many organisms...
- The interferon system and vaccinia virus evasion mechanisms
Department of Cellular and Molecular Biology, Centro Nacional de Biotecnologia, CSIC, Ciudad Universitaria Cantoblanco, Madrid, Spain
J Interferon Cytokine Res 29:581-98. 2009
..The molecular dissection of how VACV prevents the IFN response is providing important insights on our understanding of antiviral action and immune surveillance...
- Host determinant residue lysine 627 lies on the surface of a discrete, folded domain of influenza virus polymerase PB2 subunit
Grenoble Outstation, European Molecular Biology Laboratory, Grenoble, France
PLoS Pathog 4:e1000136. 2008
..Host specificity is determined by multiple sites in different viral proteins, and mutation of only a limited number of these sites can lead to inter-species transmission...
- Essential role of domain III of nonstructural protein 5A for hepatitis C virus infectious particle assembly
Department of Molecular Virology, University of Heidelberg, Heidelberg, Germany
PLoS Pathog 4:e1000035. 2008
..Since domain III of NS5A is one of the most variable regions in the HCV genome, the results suggest that viral isolates may differ in their level of virion production and thus in their level of fitness and pathogenesis...
- Viral control of mitochondrial apoptosis
INSERM, U848, Villejuif, France
PLoS Pathog 4:e1000018. 2008
..Here, we summarize the current knowledge on the viral modulation of mitochondrial apoptosis, by focusing in particular on the mechanisms by which viral proteins control the host cell death apparatus.
- The NPro product of bovine viral diarrhea virus inhibits DNA binding by interferon regulatory factor 3 and targets it for proteasomal degradation
Division of Basic Medical Sciences, St George s, University of London, London SW17 0RE, United Kingdom
J Virol 80:11723-32. 2006
..The autoprotease activity of NPro is not required for the inhibition of type I interferon induction or the targeting of IRF-3 for degradation...
- A viral protein suppresses RNA silencing and binds silencing-generated, 21- to 25-nucleotide double-stranded RNAs
Agricultural Biotechnology Center, Plant Biology Institute, PO Box 411, H 2101, Godollo, Hungary
EMBO J 21:3070-80. 2002
..Moreover, the observation that p19-expressing transgenic plants show altered leaf morphology might indicate that the p19-targeted PTGS pathway is also important in the regulation of plant development...
- The epigenetic landscape of latent Kaposi sarcoma-associated herpesvirus genomes
Heinrich Pette Institute for Experimental Virology and Immunology, Hamburg, Germany
PLoS Pathog 6:e1000935. 2010
..Our findings suggest that the modification patterns identified here induce a poised state of repression during viral latency, which can be rapidly reversed once the lytic cycle is induced...
- A concerted action of hepatitis C virus p7 and nonstructural protein 2 regulates core localization at the endoplasmic reticulum and virus assembly
Universite de Lyon, Lyon, France
PLoS Pathog 7:e1002144. 2011
..In conclusion, we identified p7 and NS2 as key determinants governing the subcellular localization of HCV core to LDs vs. ER and required for initiation of the early steps of virus assembly...
- Hepatitis C virus protease NS3/4A cleaves mitochondrial antiviral signaling protein off the mitochondria to evade innate immunity
Xiao Dong Li
Howard Hughes Medical Institute, Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9148, USA
Proc Natl Acad Sci U S A 102:17717-22. 2005
- Hepatitis C virus NS2 protein serves as a scaffold for virus assembly by interacting with both structural and nonstructural proteins
Department of Microbiology and Immunology, The University of Texas Medical Branch at Galveston, 301 University Boulevard, Galveston, TX 77555 0610, USA
J Virol 85:86-97. 2011
..p7 may play an accessory role by regulating NS2 membrane topology, which is important for NS2-mediated protein interactions and therefore NS2 function...
- Transmission of influenza virus in a mammalian host is increased by PB2 amino acids 627K or 627E/701N
Department of Microbiology, Mount Sinai School of Medicine, New York, NY, USA
PLoS Pathog 5:e1000252. 2009
..Thus, our data show that PB2 amino acids 627 and 701 are determinants of mammalian inter-host transmission in diverse virus backgrounds...
- Expression of baculovirus P35 prevents cell death in Drosophila
B A Hay
Howard Hughes Medical Institute, University of California Berkeley 94720 3200
Development 120:2121-9. 1994
..Identification of molecules that interact biochemically or genetically with P35 in Drosophila should provide important insights into how cell death is regulated...
- Analysis of the genomic sequence of a human metapneumovirus
Bernadette G van den Hoogen
Department of Virology, Erasmus Medical Center Rotterdam, Dr Molewaterplein 50, Rotterdam, 3015 GE, The Netherlands
Virology 295:119-32. 2002
- Molecular basis for high virulence of Hong Kong H5N1 influenza A viruses
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, 2015 Linden Drive, Madison, WI 53706, USA
Science 293:1840-2. 2001
..Moreover, high cleavability of the hemagglutinin glycoprotein was an essential requirement for lethal infection...
- A single N66S mutation in the PB1-F2 protein of influenza A virus increases virulence by inhibiting the early interferon response in vivo
Gina M Conenello
Mount Sinai School of Medicine, Department of Microbiology, One Gustave L Levy Place, Box 1124, New York, NY 10029, USA
J Virol 85:652-62. 2011
..These data suggest that PB1-F2 N66S may contribute to the delay of innate immune responses, allowing for unchecked viral growth and ultimately severe immunopathology observed in the lungs...
- The 1.2-megabase genome sequence of Mimivirus
Unite des Rickettsies, Faculte de Medecine, CNRS UMR6020, Universite de la Mediterranee, 13385 Marseille Cedex 05, France
Science 306:1344-50. 2004
..This new sequence data might help shed a new light on the origin of DNA viruses and their role in the early evolution of eukaryotes...
- Adaptation of pandemic H1N1 influenza viruses in mice
Natalia A Ilyushina
Department of Infectious Diseases, St Jude Children s Research Hospital, Memphis, Tennessee 38105 3678, USA
J Virol 84:8607-16. 2010
..These modulators of virulence, therefore, may have been the driving components of early evolution, which paved the way for novel 2009 viruses in mammals...
- A bacteriolytic agent that detects and kills Bacillus anthracis
Laboratory of Bacteriql Pathogenesis and Immunology, The Rockefeller University, New York, New York 10021, USA
Nature 418:884-9. 2002
..The lytic specificity of PlyG was also exploited as part of a rapid method for the identification of B. anthracis. We conclude that PlyG is a tool for the treatment and detection of B. anthracis...
- Different trajectories of parallel evolution during viral adaptation
H A Wichman
Department of Biological Sciences, University of Idaho, Moscow, ID 83844, USA
Science 285:422-4. 1999
..However, the order of these changes differed between replicates, and parallel substitutions did not reflect the changes with the largest beneficial effects or indicate a common trajectory of adaptation...
- Identification of amino acid residues critical for the anti-interferon activity of the nucleoprotein of the prototypic arenavirus lymphocytic choriomeningitis virus
Department of Immunology, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, New York 10029, USA
J Virol 83:11330-40. 2009
..In addition, A549 cells infected with rLCMV/NP*(D382A), but not with WT LCMV, produced IFN and failed to rescue replication of the IFN-sensitive Newcastle disease virus...
- Interaction of polymerase subunit PB2 and NP with importin alpha1 is a determinant of host range of influenza A virus
Institute of Virology, Philipps University Marburg, Germany
PLoS Pathog 4:e11. 2008
..These findings demonstrate that adaptation of the viral polymerase to the nuclear import machinery plays an important role in interspecies transmission of influenza virus...
- The complete genomes and proteomes of 27 Staphylococcus aureus bacteriophages
Targanta Therapeutics, 7170 Frederick Banting, Second Floor, Ville Saint Laurent, QC, Canada H4S 2A1
Proc Natl Acad Sci U S A 102:5174-9. 2005
..These phage genomes offer an exciting opportunity to discern molecular mechanisms of phage evolution and diversity...
- A Begomovirus DNAbeta-encoded protein binds DNA, functions as a suppressor of RNA silencing, and targets the cell nucleus
Institute of Biotechnology, Zhejiang University, Hangzhou 310029, People s Republic of China
J Virol 79:10764-75. 2005
- Plant viral movement proteins: agents for cell-to-cell trafficking of viral genomes
William J Lucas
Section of Plant Biology, College of Biological Sciences, University of California, One Shields Ave, Davis, CA 95616, USA
Virology 344:169-84. 2006
- Poxvirus genomes: a phylogenetic analysis
Department of Virology, Faculty of Medicine, Imperial College London, St Mary s Campus, Norfolk Place, London W2 1PG, UK
J Gen Virol 85:105-17. 2004
..This suggests that CPV-BR and GRI-90 should be separate species...
- Transcriptomic analysis of host immune and cell death responses associated with the influenza A virus PB1-F2 protein
Ronan Le Goffic
Unite de Virologie et Immunologie Moleculaires, UR 892 INRA, Jouy en Josas, France
PLoS Pathog 7:e1002202. 2011
..Collectively, these data demonstrate that PB1-F2 strongly influences the early host response during IAV infection and provides new insights into the mechanisms by which PB1-F2 mediates virulence...
- Human cytomegalovirus and mucoepidermoid carcinoma of salivary glands: cell-specific localization of active viral and oncogenic signaling proteins is confirmatory of a causal relationship
Laboratory for Developmental Genetics, USC, Los Angeles, CA 90089 0641, USA
Exp Mol Pathol 92:118-25. 2012
..In the absence of any contrary evidence, hCMV can reasonably be designated an "oncovirus."..
- Evidence for a role of the cellular ND10 protein PML in mediating intrinsic immunity against human cytomegalovirus infections
Institut für Klinische und Molekulare Virologie der Universität Erlangen Nürnberg, Schlossgarten 4, 91054 Erlangen, Germany
J Virol 80:8006-18. 2006
..These results strongly suggest that PML functions as part of an intrinsic immune mechanism against cytomegalovirus infections...
- The influenza virus protein PB1-F2 inhibits the induction of type I interferon at the level of the MAVS adaptor protein
Zsuzsanna T Varga
Department of Microbiology, Mount Sinai School of Medicine, New York City, New York, United States of America
PLoS Pathog 7:e1002067. 2011
..In summary, we have characterized the anti-interferon function of PB1-F2 and we suggest that this activity contributes to the enhanced pathogenicity seen with PB1-F2 N66S- expressing influenza viruses...
- Characterization of the human cytomegalovirus gH/gL/UL128-131 complex that mediates entry into epithelial and endothelial cells
Brent J Ryckman
6366 Basic Sciences Building, Mail Code L 220, Department of Molecular Microbiology and Immunology, Oregon Health and Sciences University, 3181 Sam Jackson Park Rd, Portland, OR 97239, USA
J Virol 82:60-70. 2008
..Thus, we concluded that UL128, UL130, and UL131 must all bind simultaneously onto gH/gL for the production of complexes that can function in entry into epithelial and endothelial cells...
- Human metapneumovirus glycoprotein G inhibits TLR4-dependent signaling in monocyte-derived dendritic cells
Department of Pediatrics, University of Texas Medical Branch, Galveston, TX 77555, USA
J Immunol 187:47-54. 2011
..Our results demonstrate that hMPV G protein plays an important role in inhibiting host innate immune responses, likely affecting adaptive responses too...
- Viral disorder or disordered viruses: do viral proteins possess unique features?
Center for Computational Biology and Bioinformatics, Indiana University School of Medicine, Indianapolis, IN 46202, USA
Protein Pept Lett 17:932-51. 2010
..To sustain these fast environmental changes, viral proteins elaborated multiple measures, from relatively low van der Waals contact densities, to inclusion of a large ..
- Norovirus regulation of the innate immune response and apoptosis occurs via the product of the alternative open reading frame 4
Section of Virology, Department of Medicine, Imperial College London, London, United Kingdom
PLoS Pathog 7:e1002413. 2011
..The immune regulatory function of the MNV VF1 protein provide important perspectives for future research into norovirus biology and pathogenesis...
- Polydnavirus Ank proteins bind NF-κB homodimers and inhibit processing of Relish
Department of Entomology, University of Georgia, Athens, Georgia, USA
PLoS Pathog 8:e1002722. 2012
..Overall, our data provide the first quantitative measures of insect and viral IκB binding affinities, while also showing that viral mimics disable Relish processing...
- Nuclear import of the influenza A virus transcriptional machinery
E C Hutchinson
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, United Kingdom
Vaccine 30:7353-8. 2012
..In this review we discuss how the virus exploits nuclear import pathways to allow regulated and chaperoned assembly of RNPs in the nucleus, and describe how the import machinery itself can be a determinant of host tropism...
- The phage lambda major tail protein structure reveals a common evolution for long-tailed phages and the type VI bacterial secretion system
Lisa G Pell
Department of Biochemistry, University of Toronto, Medical Sciences Building, Toronto, ON M5S 1A8, Canada
Proc Natl Acad Sci U S A 106:4160-5. 2009
..Using Hcp1 as a model, we propose a polymerization mechanism for gpV involving several disorder-to-order transitions...