repressor proteins

Summary

Summary: Proteins which are normally bound to the operator locus of an operon, thereby preventing transcription of the structural genes. In enzyme induction, the substrate of the inducible enzyme binds to the repressor protein, causing its release from the operator and freeing the structural genes for transcription. In enzyme repression, the end product of the enzyme sequence binds to the free repressor protein, the resulting complex then binds to the operator and prevents transcription of the structural genes.

Top Publications

  1. pmc Functional demarcation of active and silent chromatin domains in human HOX loci by noncoding RNAs
    John L Rinn
    Program in Epithelial Biology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Cell 129:1311-23. 2007
  2. pmc The Polycomb complex PRC2 and its mark in life
    Raphael Margueron
    Institut Curie, 26 Rue d Ulm, 75005 Paris, France
    Nature 469:343-9. 2011
  3. ncbi Snail, Zeb and bHLH factors in tumour progression: an alliance against the epithelial phenotype?
    Hector Peinado
    Departamento de Bioquimica, Facultad de Medicina, Universidad Autonoma de Madrid UAM, Instituto de Investigaciones Biomedicas Alberto Sols CSIC UAM, Arturo Duperier 4, 28029 Madrid, Spain
    Nat Rev Cancer 7:415-28. 2007
  4. ncbi Role of histone H3 lysine 27 methylation in Polycomb-group silencing
    Ru Cao
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7295, USA
    Science 298:1039-43. 2002
  5. ncbi Isolation of a human gene that inhibits HIV-1 infection and is suppressed by the viral Vif protein
    Ann M Sheehy
    Department of Microbiology, The Children s Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Nature 418:646-50. 2002
  6. ncbi The transcription factor snail controls epithelial-mesenchymal transitions by repressing E-cadherin expression
    A Cano
    Instituto de Investigaciones Biomedicas, Arturo Duperier 4, 28029 Madrid, Spain
    Nat Cell Biol 2:76-83. 2000
  7. ncbi Histone demethylation mediated by the nuclear amine oxidase homolog LSD1
    Yujiang Shi
    Department of Pathology, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Cell 119:941-53. 2004
  8. pmc Genomewide analysis of PRC1 and PRC2 occupancy identifies two classes of bivalent domains
    Manching Ku
    Molecular Pathology Unit and Center for Cancer Research, Massachusetts General Hospital, Charlestown, MA, USA
    PLoS Genet 4:e1000242. 2008
  9. ncbi The polycomb group protein EZH2 is involved in progression of prostate cancer
    Sooryanarayana Varambally
    Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    Nature 419:624-9. 2002
  10. ncbi Genome-wide mapping of in vivo protein-DNA interactions
    David S Johnson
    Department of Genetics, Stanford University School of Medicine, Stanford, CA, 94305 5120, USA
    Science 316:1497-502. 2007

Detail Information

Publications261 found, 100 shown here

  1. pmc Functional demarcation of active and silent chromatin domains in human HOX loci by noncoding RNAs
    John L Rinn
    Program in Epithelial Biology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Cell 129:1311-23. 2007
    ..Thus, transcription of ncRNA may demarcate chromosomal domains of gene silencing at a distance; these results have broad implications for gene regulation in development and disease states...
  2. pmc The Polycomb complex PRC2 and its mark in life
    Raphael Margueron
    Institut Curie, 26 Rue d Ulm, 75005 Paris, France
    Nature 469:343-9. 2011
    ..Recent studies of PRC2 have expanded our perspectives on its function and regulation, and uncovered a role for non-coding RNA in the recruitment of PRC2 to target genes...
  3. ncbi Snail, Zeb and bHLH factors in tumour progression: an alliance against the epithelial phenotype?
    Hector Peinado
    Departamento de Bioquimica, Facultad de Medicina, Universidad Autonoma de Madrid UAM, Instituto de Investigaciones Biomedicas Alberto Sols CSIC UAM, Arturo Duperier 4, 28029 Madrid, Spain
    Nat Rev Cancer 7:415-28. 2007
    ....
  4. ncbi Role of histone H3 lysine 27 methylation in Polycomb-group silencing
    Ru Cao
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7295, USA
    Science 298:1039-43. 2002
    ..Methylation on H3-K27 facilitates binding of Polycomb (PC), a component of the PRC1 complex, to histone H3 amino-terminal tail. Thus, these studies establish a link between histone methylation and PcG-mediated gene silencing...
  5. ncbi Isolation of a human gene that inhibits HIV-1 infection and is suppressed by the viral Vif protein
    Ann M Sheehy
    Department of Microbiology, The Children s Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Nature 418:646-50. 2002
    ..Because the Vif:CEM15 regulatory circuit is critical for HIV-1 replication, perturbing the circuit may be a promising target for future HIV/AIDS therapies...
  6. ncbi The transcription factor snail controls epithelial-mesenchymal transitions by repressing E-cadherin expression
    A Cano
    Instituto de Investigaciones Biomedicas, Arturo Duperier 4, 28029 Madrid, Spain
    Nat Cell Biol 2:76-83. 2000
    ..Snail may thus be considered as a marker for malignancy, opening up new avenues for the design of specific anti-invasive drugs...
  7. ncbi Histone demethylation mediated by the nuclear amine oxidase homolog LSD1
    Yujiang Shi
    Department of Pathology, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Cell 119:941-53. 2004
    ..The results thus identify a histone demethylase conserved from S. pombe to human and reveal dynamic regulation of histone methylation by both histone methylases and demethylases...
  8. pmc Genomewide analysis of PRC1 and PRC2 occupancy identifies two classes of bivalent domains
    Manching Ku
    Molecular Pathology Unit and Center for Cancer Research, Massachusetts General Hospital, Charlestown, MA, USA
    PLoS Genet 4:e1000242. 2008
    ..We propose that large CpG islands depleted of activating motifs confer epigenetic memory by recruiting the full repertoire of Polycomb complexes in pluripotent cells...
  9. ncbi The polycomb group protein EZH2 is involved in progression of prostate cancer
    Sooryanarayana Varambally
    Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA
    Nature 419:624-9. 2002
    ..Thus, dysregulated expression of EZH2 may be involved in the progression of prostate cancer, as well as being a marker that distinguishes indolent prostate cancer from those at risk of lethal progression...
  10. ncbi Genome-wide mapping of in vivo protein-DNA interactions
    David S Johnson
    Department of Genetics, Stanford University School of Medicine, Stanford, CA, 94305 5120, USA
    Science 316:1497-502. 2007
    ..96] and statistical confidence (P <10(-4)), properties that were important for inferring new candidate interactions. These include key transcription factors in the gene network that regulates pancreatic islet cell development...
  11. ncbi JAZ repressor proteins are targets of the SCF(COI1) complex during jasmonate signalling
    Bryan Thines
    Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164 6340, USA
    Nature 448:661-5. 2007
    ....
  12. pmc Polycomb proteins targeted by a short repeat RNA to the mouse X chromosome
    Jing Zhao
    Howard Hughes Medical Institute, Boston, MA 02115, USA
    Science 322:750-6. 2008
    ..Likewise, PRC2 deficiency compromises Xist up-regulation. Therefore, RepA, together with PRC2, is required for the initiation and spread of XCI. We conclude that a ncRNA cofactor recruits Polycomb complexes to their target locus...
  13. ncbi The JAZ family of repressors is the missing link in jasmonate signalling
    A Chini
    Departamento de Genética Molecular de Plantas, Centro Nacional de Biotecnologia CSIC, Campus Universidad Autonoma, 28049 Madrid, Spain
    Nature 448:666-71. 2007
    ..Furthermore, we demonstrate the existence of a regulatory feed-back loop involving MYC2 and JAZ proteins, which provides a mechanistic explanation for the pulsed response to jasmonate and the subsequent desensitization of the cell...
  14. ncbi Mechanisms of polycomb gene silencing: knowns and unknowns
    Jeffrey A Simon
    Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis 55455, USA
    Nat Rev Mol Cell Biol 10:697-708. 2009
    ..These findings have an impact on the epigenetic programming of gene expression in many biological systems...
  15. ncbi The transcription factor snail is a repressor of E-cadherin gene expression in epithelial tumour cells
    E Batlle
    Unitat de Biologia Cel lular i Molecular, Institut Municipal d Investigacio Medica, Universitat Pompeu Fabra, Dr Aiguader 80, 08003 Barcelona, Spain
    Nat Cell Biol 2:84-9. 2000
    ..Inhibition of Snail function in epithelial cancer cell lines lacking E-cadherin protein restores the expression of the E-cadherin gene...
  16. ncbi The small RNA chaperone Hfq and multiple small RNAs control quorum sensing in Vibrio harveyi and Vibrio cholerae
    Derrick H Lenz
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Cell 118:69-82. 2004
    ..We propose that Hfq, together with these sRNAs, creates an ultrasensitive regulatory switch that controls the critical transition into the high cell density, quorum-sensing mode...
  17. pmc Genome-wide mapping of Polycomb target genes unravels their roles in cell fate transitions
    Adrian P Bracken
    Biotech Research and Innovation Centre BRIC, 2100 Copenhagen Ø, Denmark
    Genes Dev 20:1123-36. 2006
    ....
  18. pmc Waves of retrotransposon expansion remodel genome organization and CTCF binding in multiple mammalian lineages
    Dominic Schmidt
    Cancer Research UK, Cambridge Research Institute, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, UK
    Cell 148:335-48. 2012
    ..Repeat-driven dispersal of CTCF binding is a fundamental, ancient, and still highly active mechanism of genome evolution in mammalian lineages...
  19. pmc Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter
    L M Guzman
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Bacteriol 177:4121-30. 1995
    ..We have exploited the tight regulation of the PBAD promoter to study the phenotypes of null mutations of essential genes and explored the use of pBAD vectors as an expression system...
  20. ncbi Lineage-specific polycomb targets and de novo DNA methylation define restriction and potential of neuronal progenitors
    Fabio Mohn
    Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058 Basel, Switzerland
    Mol Cell 30:755-66. 2008
    ..These data suggest a model how de novo DNA methylation and dynamic switches in Polycomb targets restrict pluripotency and define the developmental potential of progenitor cells...
  21. pmc Origins of specificity in protein-DNA recognition
    Remo Rohs
    Howard Hughes Medical Institute, Center for Computational Biology and Bioinformatics, Columbia University, New York, NY 10032, USA
    Annu Rev Biochem 79:233-69. 2010
    ....
  22. pmc Histone deacetylases (HDACs): characterization of the classical HDAC family
    Annemieke J M de Ruijter
    Academic Medical Centre, University of Amsterdam, Amsterdam, The, Netherlands
    Biochem J 370:737-49. 2003
    ..This challenging field has generated many fascinating results which will ultimately lead to a better understanding of the mechanism of gene transcription as a whole...
  23. pmc The bifunctional microRNA miR-9/miR-9* regulates REST and CoREST and is downregulated in Huntington's disease
    Amy N Packer
    Department of Internal Medicine, University of Iowa, Iowa City, Iowa 52242, USA
    J Neurosci 28:14341-6. 2008
    ..These data provide evidence for a double negative feedback loop between the REST silencing complex and the miRNAs it regulates...
  24. ncbi Role of histone H2A ubiquitination in Polycomb silencing
    Hengbin Wang
    Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
    Nature 431:873-8. 2004
    ..Thus, our studies identify the H2A ubiquitin ligase, and link H2A ubiquitination to Polycomb silencing...
  25. ncbi Genome regulation by polycomb and trithorax proteins
    Bernd Schuettengruber
    Institute of Human Genetics, CNRS, 141, rue de la Cardonille, 34396 Montpellier Cedex 5, France
    Cell 128:735-45. 2007
    ..Recent work suggests that PcG proteins regulate the nuclear organization of their target genes and that PcG-mediated gene silencing involves noncoding RNAs and the RNAi machinery...
  26. ncbi Polycomb complexes repress developmental regulators in murine embryonic stem cells
    Laurie A Boyer
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nature 441:349-53. 2006
    ..Our results indicate that dynamic repression of developmental pathways by Polycomb complexes may be required for maintaining ES cell pluripotency and plasticity during embryonic development...
  27. ncbi The two-handed E box binding zinc finger protein SIP1 downregulates E-cadherin and induces invasion
    J Comijn
    Molecular Cell Biology Unit, Department of Molecular Biology, VIB Ghent University, B 9000, Ghent, Belgium
    Mol Cell 7:1267-78. 2001
    ..SIP1 therefore appears to be a promoter of invasion in malignant epithelial tumors...
  28. ncbi Histone methyltransferase activity of a Drosophila Polycomb group repressor complex
    Jurg Muller
    EMBL, Gene Expression Programme, Meyerhofstr 1, 69117 Heidelberg, Germany
    Cell 111:197-208. 2002
    ..These results identify E(Z) as a PcG protein with enzymatic activity and implicate histone methylation in PcG-mediated silencing...
  29. pmc Histone methyltransferase activity associated with a human multiprotein complex containing the Enhancer of Zeste protein
    Andrei Kuzmichev
    Howard Hughes Medical Institute, Division of Nucleic Acids Enzymology, Department of Biochemistry, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    Genes Dev 16:2893-905. 2002
    ..The presence of Su(z)12, a strong suppressor of position effect variegation, in PRC2 suggests that PRC2 may play a widespread role in heterochromatin-mediated silencing...
  30. pmc A small RNA regulates the expression of genes involved in iron metabolism in Escherichia coli
    Eric Massé
    Laboratory of Molecular Biology, National Cancer Institute, Building 37, Room 5132, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 99:4620-5. 2002
    ....
  31. pmc Pharmacologic disruption of Polycomb-repressive complex 2-mediated gene repression selectively induces apoptosis in cancer cells
    Jing Tan
    Molecular Pharmacology, Genome Institute of Singapore, 138672, Singapore
    Genes Dev 21:1050-63. 2007
    ..Our results demonstrate the unique feature of DZNep as a novel chromatin remodeling compound and suggest that pharmacologic reversal of PRC2-mediated gene repression by DZNep may constitute a novel approach for cancer therapy...
  32. pmc Suz12 is essential for mouse development and for EZH2 histone methyltransferase activity
    Diego Pasini
    European Institute of Oncology, Milan, Italy
    EMBO J 23:4061-71. 2004
    ..In conclusion, our data demonstrate an essential role of SUZ12 in regulating the activity of the PRC2/3 complexes, which are required for regulating proliferation and embryogenesis...
  33. pmc CTCF: master weaver of the genome
    Jennifer E Phillips
    Department of Biology, Emory University, Atlanta, GA 30322, USA
    Cell 137:1194-211. 2009
    ....
  34. pmc TRB3, a novel ER stress-inducible gene, is induced via ATF4-CHOP pathway and is involved in cell death
    Nobumichi Ohoka
    Department of Molecular Health Sciences, Graduate School of Pharmaceutical Sciences, Nagoya City University, Mizuho, Nagoya, Japan
    EMBO J 24:1243-55. 2005
    ..These results indicate that TRB3 is a novel target of CHOP/ATF4 and downregulates its own induction by repression of CHOP/ATF4 functions, and that it is involved in CHOP-dependent cell death during ER stress...
  35. pmc CTCF-mediated functional chromatin interactome in pluripotent cells
    Lusy Handoko
    Genome Institute of Singapore, Singapore
    Nat Genet 43:630-8. 2011
    ..This highly complex nuclear organization offers insights toward the unifying principles that govern genome plasticity and function...
  36. pmc GC-rich sequence elements recruit PRC2 in mammalian ES cells
    Eric M Mendenhall
    Howard Hughes Medical Institute and Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS Genet 6:e1001244. 2010
    ....
  37. pmc The polycomb group protein Suz12 is required for embryonic stem cell differentiation
    Diego Pasini
    Centre for Epigenetics and BRIC, University of Copenhagen, Ole Maaløes Vej 5, 2200 Copenhagen, Denmark
    Mol Cell Biol 27:3769-79. 2007
    ..Furthermore, we provide evidence that PcGs have different mechanisms to regulate transcription during cellular differentiation...
  38. pmc Cellular defenses against superoxide and hydrogen peroxide
    James A Imlay
    Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
    Annu Rev Biochem 77:755-76. 2008
    ..Current research seeks to identify these, and bacteria comprise an exceptionally accessible experimental system that has provided many of the answers. This manuscript reviews recent developments and identifies remaining puzzles...
  39. pmc neurogenin3 is required for the development of the four endocrine cell lineages of the pancreas
    G Gradwohl
    Institut de Genetique et de Biologie Moleculaire et Cellulaire, Centre National de la Recherche Scientifique Institut National de la Santé et de la Recherche Médicale Université Louis Pasteur, F 67404 Illkirch, France
    Proc Natl Acad Sci U S A 97:1607-11. 2000
    ..Thus, ngn3 is required for the specification of a common precursor for the four pancreatic endocrine cell types...
  40. pmc The ZEB/miR-200 feedback loop--a motor of cellular plasticity in development and cancer?
    Simone Brabletz
    Department of Visceral Surgery, University of Freiburg, Hugstetterstrasse 55, 79106 Freiburg, Germany
    EMBO Rep 11:670-7. 2010
    ....
  41. ncbi Drosophila enhancer of Zeste/ESC complexes have a histone H3 methyltransferase activity that marks chromosomal Polycomb sites
    Birgit Czermin
    Adolf Butenandt Institut, University of Munich, Schillerstrasse 44, 80336 Munich, Germany
    Cell 111:185-96. 2002
    ..Histone H3 methylated in vitro by the E(Z)/ESC complex binds specifically to Polycomb protein...
  42. pmc SETDB1: a novel KAP-1-associated histone H3, lysine 9-specific methyltransferase that contributes to HP1-mediated silencing of euchromatic genes by KRAB zinc-finger proteins
    David C Schultz
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    Genes Dev 16:919-32. 2002
    ..Thus, KAP-1 is a molecular scaffold that is targeted by KRAB-ZFPs to specific loci and coordinates both histone methylation and the deposition of HP1 proteins to silence gene expression...
  43. ncbi Transcriptional repression by the methyl-CpG-binding protein MeCP2 involves a histone deacetylase complex
    X Nan
    Institute of Cell and Molecular Biology, University of Edinburgh, UK
    Nature 393:386-9. 1998
    ..The data suggest that two global mechanisms of gene regulation, DNA methylation and histone deacetylation, can be linked by MeCP2...
  44. ncbi The snail superfamily of zinc-finger transcription factors
    M Angela Nieto
    Instituto Cajal, Doctor Arce, 37, 28002 Madrid, Spain
    Nat Rev Mol Cell Biol 3:155-66. 2002
    ..Different family members have also been implicated in the signalling cascade that confers left right identity, as well as in the formation of appendages, neural differentiation, cell division and cell survival...
  45. ncbi Localization of ASH1 mRNA particles in living yeast
    E Bertrand
    Department of Anatomy and Structural Biology and Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Mol Cell 2:437-45. 1998
    ..Video microscopy demonstrated that She1p/Myo4p moved particles to the bud tip at 200-440 nm/sec. Therefore, the ASH1 3'UTR-dependent particle serves as a marker for RNA transport and localization...
  46. ncbi An epidemic, toxin gene-variant strain of Clostridium difficile
    L Clifford McDonald
    Epidemiology and Laboratory Branch, Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, USA
    N Engl J Med 353:2433-41. 2005
    ..difficile with increased virulence, resistance, or both...
  47. ncbi A mouse Mecp2-null mutation causes neurological symptoms that mimic Rett syndrome
    J Guy
    Wellcome Centre for Cell Biology, Institute of Cell and Molecular Biology, University of Edinburgh, The King s Buildings, Edinburgh, UK
    Nat Genet 27:322-6. 2001
    ....
  48. pmc The TetR family of transcriptional repressors
    Juan L Ramos
    Department of Plant Biochemistry and Molecular and Cellular Biology, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, Granada, Spain
    Microbiol Mol Biol Rev 69:326-56. 2005
    ..Information related to the TetR family of regulators has been updated in a database that can be accessed at www.bactregulators.org...
  49. ncbi Circular chromosome conformation capture (4C) uncovers extensive networks of epigenetically regulated intra- and interchromosomal interactions
    Zhihu Zhao
    Department of Development and Genetics, Evolution Biology Centre, Uppsala University, Norbyvagen 18A, SE 752 36 Uppsala, Sweden
    Nat Genet 38:1341-7. 2006
    ..These observations shed new light on development, cancer epigenetics and the evolution of imprinting...
  50. pmc Batf3 deficiency reveals a critical role for CD8alpha+ dendritic cells in cytotoxic T cell immunity
    Kai Hildner
    Department of Pathology and Immunology, Washington University School of Medicine, 660 South Euclid Avenue, St Louis, MO 63110, USA
    Science 322:1097-100. 2008
    ..Importantly, rejection of highly immunogenic syngeneic tumors was impaired in Batf3-/- mice. These results suggest an important role for CD8alpha+ dendritic cells and cross-presentation in responses to viruses and in tumor rejection...
  51. ncbi Deficiency of methyl-CpG binding protein-2 in CNS neurons results in a Rett-like phenotype in mice
    R Z Chen
    The Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    Nat Genet 27:327-31. 2001
    ..Mecp2 deficiency in these neurons is sufficient to cause neuronal dysfunction with symptomatic manifestation similar to Rett syndrome...
  52. ncbi The HPV-16 E6 and E6-AP complex functions as a ubiquitin-protein ligase in the ubiquitination of p53
    M Scheffner
    Laboratory of Tumor Virus Biology, National Cancer Institute, Bethesda, Maryland 20892
    Cell 75:495-505. 1993
    ..The ubiquitination of p53 requires the E1 enzyme and a novel E2 in mammalian cells, while E3 activity is conferred by the E6-E6-AP complex. Furthermore, E6-AP appears to have ubiquitin-protein ligase activity in the absence of E6...
  53. ncbi HES and HERP families: multiple effectors of the Notch signaling pathway
    Tatsuya Iso
    Institute for Genetic Medicine, Department of Biochemistry and Molecular Biology, Keck School of Medicine of the University of Southern California, Los Angeles, California 90089, USA
    J Cell Physiol 194:237-55. 2003
    ..The identification of the HERP family as a Notch effector that cooperates with HES/E(spl) family has opened a new avenue to our understanding of the Notch signaling pathway...
  54. pmc Role of the polycomb protein EED in the propagation of repressive histone marks
    Raphael Margueron
    Howard Hughes Medical Institute and Department of Biochemistry, New York University Medical School, 522 First Avenue, New York, New York 10016, USA
    Nature 461:762-7. 2009
    ..These findings suggest a model for the propagation of the H3K27me3 mark that accounts for the maintenance of repressive chromatin domains and for the transmission of a histone modification from mother to daughter cells...
  55. pmc Phosphorylation of the PRC2 component Ezh2 is cell cycle-regulated and up-regulates its binding to ncRNA
    Syuzo Kaneko
    Howard Hughes Medical Institute, New York University School of Medicine, New York, 10016, USA
    Genes Dev 24:2615-20. 2010
    ..A phospho-mimic at residue 345 increased HOTAIR ncRNA binding to Ezh2, while the phospho-mimic at residue 487 was ineffectual. An Ezh2 domain comprising T345 was found to be important for binding to HOTAIR and the 5' end of Xist...
  56. pmc Polycomb complexes act redundantly to repress genomic repeats and genes
    Martin Leeb
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, 1030 Vienna, Austria
    Genes Dev 24:265-76. 2010
    ..This indicates a contribution of the Polycomb group system to the defense against parasitic DNA, and a potential role of genomic repeats in Polycomb-mediated gene regulation...
  57. ncbi ATM signaling facilitates repair of DNA double-strand breaks associated with heterochromatin
    Aaron A Goodarzi
    Genome Damage and Stability Centre, University of Sussex, East Sussex BN1 9RQ, UK
    Mol Cell 31:167-77. 2008
    ..These data suggest that the importance of ATM signaling for DSB repair increases as the heterochromatic component of a genome expands...
  58. ncbi Hedgehog signaling and Bmi-1 regulate self-renewal of normal and malignant human mammary stem cells
    Suling Liu
    Comprehensive Cancer Center, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan 48109 0946, USA
    Cancer Res 66:6063-71. 2006
    ..These studies support a cancer stem cell model in which the hedgehog pathway and Bmi-1 play important roles in regulating self-renewal of normal and tumorigenic human mammary stem cells...
  59. ncbi Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis
    T Lee
    Department of Biological Sciences, Stanford University, California 94305, USA
    Neuron 22:451-61. 1999
    ..As an example, we show that the short stop gene is required in mushroom body neurons for the extension and guidance of their axons...
  60. pmc Dynamic equilibrium and heterogeneity of mouse pluripotent stem cells with distinct functional and epigenetic states
    Katsuhiko Hayashi
    Wellcome Trust Cancer Research UK Gurdon Institute, The Henry Wellcome Building of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Cell Stem Cell 3:391-401. 2008
    ....
  61. ncbi Methylated DNA and MeCP2 recruit histone deacetylase to repress transcription
    P L Jones
    Laboratory of Molecular Embryology, National Institute of Child Health and Human Development, National Institute of Health, Bethesda, Maryland 20892 5431, USA
    Nat Genet 19:187-91. 1998
    ..These results establish a direct causal relationship between DNA methylation-dependent transcriptional silencing and the modification of chromatin...
  62. ncbi Bmi-1 is a novel molecular marker of nasopharyngeal carcinoma progression and immortalizes primary human nasopharyngeal epithelial cells
    Li Bing Song
    State Key Laboratory of Oncology in Southern China, Sun Yat sen University Cancer Center, Guangzhou, China
    Cancer Res 66:6225-32. 2006
    ....
  63. ncbi Targeting of the Bmi-1 oncogene/stem cell renewal factor by microRNA-128 inhibits glioma proliferation and self-renewal
    Jakub Godlewski
    Dardinger Laboratory for Neuro oncology and Neurosciences, Department of Neurological Surgery, The Ohio State University Medical Center and James Comprehensive Cancer Center, Columbus, Ohio 43210
    Cancer Res 68:9125-30. 2008
    ....
  64. pmc A CTCF-independent role for cohesin in tissue-specific transcription
    Dominic Schmidt
    Cancer Research UK, Cambridge Research Institute, Li Ka Shing Centre, Cambridge CB2 0RE, United Kingdom
    Genome Res 20:578-88. 2010
    ....
  65. pmc Microarray analysis identifies a death-from-cancer signature predicting therapy failure in patients with multiple types of cancer
    Gennadi V Glinsky
    Sidney Kimmel Cancer Center, San Diego, California 92121, USA
    J Clin Invest 115:1503-21. 2005
    ....
  66. pmc A natural antisense transcript regulates Zeb2/Sip1 gene expression during Snail1-induced epithelial-mesenchymal transition
    Manuel Beltran
    Programa de Recerca en Càncer, Institut Municipal d Investigació Mèdica IMIM Hospital del Mar, Barcelona 08003, Spain
    Genes Dev 22:756-69. 2008
    ..Therefore, the results presented in this article reveal the existence of a NAT capable of activating Zeb2 expression, explain the mechanism involved in this activation, and demonstrate that this NAT regulates E-cadherin expression...
  67. pmc Ezh1 and Ezh2 maintain repressive chromatin through different mechanisms
    Raphael Margueron
    Department of Biochemistry, New York University Medical School, 522 First Avenue, New York, NY 10016, USA
    Mol Cell 32:503-18. 2008
    ..These results might reflect subfunctionalization of a PcG protein during evolution...
  68. ncbi Chromatin signatures of pluripotent cell lines
    Veronique Azuara
    Lymphocyte Development Group, MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital, Du Cane Road, London W12 ONN, UK
    Nat Cell Biol 8:532-8. 2006
    ..Our data suggest that lineage-specific genes are primed for expression in ES cells but are held in check by opposing chromatin modifications...
  69. ncbi A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity
    Na Yu Chia
    Gene Regulation Laboratory, Genome Institute of Singapore, 60 Biopolis Street, Singapore 138672
    Nature 468:316-20. 2010
    ..Altogether, our study uncovers a wealth of novel hESC regulators wherein PRDM14 exemplifies a key transcription factor required for the maintenance of hESC identity and the reacquisition of pluripotency in human somatic cells...
  70. ncbi Histone methylation by PRC2 is inhibited by active chromatin marks
    Frank W Schmitges
    Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, CH 4058 Basel, Switzerland
    Mol Cell 42:330-41. 2011
    ..Inhibition by active chromatin marks, coupled to stimulation by transcriptionally repressive H3K27me3, enables PRC2 to autonomously template repressive H3K27me3 without overwriting active chromatin domains...
  71. pmc Quorum-sensing regulators control virulence gene expression in Vibrio cholerae
    Jun Zhu
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 99:3129-34. 2002
    ..Together these data suggest a role for quorum sensing in modulating expression of blocks of virulence genes in a reciprocal fashion in vivo...
  72. ncbi Polycomb group proteins: navigators of lineage pathways led astray in cancer
    Adrian P Bracken
    The Smurfit Institute of Genetics, Trinity College Dublin and The Adelaide and Meath Hospital, including The National Children s Hospital, Dublin, Ireland
    Nat Rev Cancer 9:773-84. 2009
    ..We also speculate about how their deregulation contributes to tumorigenesis...
  73. pmc Many human large intergenic noncoding RNAs associate with chromatin-modifying complexes and affect gene expression
    Ahmad M Khalil
    The Broad Institute of Harvard and Massachusetts Institute of Technology, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 106:11667-72. 2009
    ..We propose a model in which some lincRNAs guide chromatin-modifying complexes to specific genomic loci to regulate gene expression...
  74. ncbi KAP1 controls endogenous retroviruses in embryonic stem cells
    Helen M Rowe
    School of Life Sciences, Ecole Polytechnique Federale de Lausanne, 1015 Lausanne, Switzerland
    Nature 463:237-40. 2010
    ..Correspondingly, IAP 5'UTR sequences can impose in cis KAP1-dependent repression on a heterologous promoter in ES cells. Our results establish that KAP1 controls endogenous retroelements during early embryonic development...
  75. pmc Recruitment of chromatin-modifying enzymes by CTIP2 promotes HIV-1 transcriptional silencing
    Céline Marban
    INSERM unité 575 Pathophysiology of Nervous System, Centre de Neurochimie, Strasbourg, France
    EMBO J 26:412-23. 2007
    ..Altogether, our findings uncover new therapeutic opportunities for purging latent HIV-1 viruses from their cellular reservoirs...
  76. pmc YY1 tethers Xist RNA to the inactive X nucleation center
    Yesu Jeon
    Howard Hughes Medical Institute, Department of Molecular Biology, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, Boston, MA 02114, USA
    Cell 146:119-33. 2011
    ..Specific YY1-to-RNA and YY1-to-DNA contacts are required to load Xist particles onto X. YY1 interacts with Xist RNA through Repeat C. We propose that YY1 acts as adaptor between regulatory RNA and chromatin targets...
  77. pmc Opposing effects of polyglutamine expansion on native protein complexes contribute to SCA1
    Janghoo Lim
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Nature 452:713-8. 2008
    ..This model provides mechanistic insight into the molecular pathogenesis of SCA1 as well as other polyglutamine diseases...
  78. pmc Jarid2/Jumonji coordinates control of PRC2 enzymatic activity and target gene occupancy in pluripotent cells
    Jamy C Peng
    Department of Chemical and Systems Biology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Cell 139:1290-302. 2009
    ..Our findings illuminate a mechanism of histone methylation regulation in pluripotent cells and during early cell-fate transitions...
  79. ncbi JARID2 regulates binding of the Polycomb repressive complex 2 to target genes in ES cells
    Diego Pasini
    Biotech Research and Innovation Centre BRIC, University of Copenhagen, Ole Maaløes Vej 5, 2200 Copenhagen N, Denmark
    Nature 464:306-10. 2010
    ..Thus, these results demonstrate that JARID2 is essential for the binding of PcG proteins to target genes and, consistent with this, for the proper differentiation of embryonic stem cells and normal development...
  80. pmc Peripheral CD103+ dendritic cells form a unified subset developmentally related to CD8alpha+ conventional dendritic cells
    Brian T Edelson
    Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO 63110, USA
    J Exp Med 207:823-36. 2010
    ..These data provide evidence for a developmental relationship between lymphoid organ-resident CD8alpha+ cDCs and nonlymphoid CD103+ DCs...
  81. pmc A region of the human HOXD cluster that confers polycomb-group responsiveness
    Caroline J Woo
    Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA
    Cell 140:99-110. 2010
    ..We conclude that D11.12 is a Polycomb-dependent regulatory region with similarities to Drosophila PREs, indicating conservation in the mechanisms that target PcG function in mammals and flies...
  82. pmc Ring1B compacts chromatin structure and represses gene expression independent of histone ubiquitination
    Ragnhild Eskeland
    MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Crewe Road, Edinburgh EH4 2XU, UK
    Mol Cell 38:452-64. 2010
    ..We suggest that Ring1B-mediated chromatin compaction acts to directly limit transcription in vivo...
  83. ncbi Signalling downstream of activated mammalian Notch
    S Jarriault
    Unité de Biologie Moléculaire de l Expression Génique, URA 1149 CNRS, Institut Pasteur, Paris, France
    Nature 377:355-8. 1995
    ..Here we show that activated forms of mNotch associate with the human analogue of Su(H), KBF2/RBP-J kappa (refs 8,9) and act as transcriptional activators through the KBF2-binding sites of the HES-1 promoter...
  84. ncbi Differentiation of mouse embryonic stem cells into a defined neuronal lineage
    Miriam Bibel
    Friedrich Miescher Institute, CH 4058 Basel, Switzerland
    Nat Neurosci 7:1003-9. 2004
    ..As they do in vivo, these cells went on to generate neurons with remarkably uniform biochemical and electrophysiological characteristics...
  85. ncbi Methylation of a CTCF-dependent boundary controls imprinted expression of the Igf2 gene
    A C Bell
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0540, USA
    Nature 405:482-5. 2000
    ..Our results reveal that DNA methylation can control gene expression by modulating enhancer access to the gene promoter through regulation of an enhancer boundary...
  86. pmc Jumonji modulates polycomb activity and self-renewal versus differentiation of stem cells
    Xiaohua Shen
    Department of Pediatric Oncology, Dana Farber Cancer Institute, Children s Hospital, and Harvard Medical School, Boston, MA 02115, USA
    Cell 139:1303-14. 2009
    ..Our results demonstrate that dynamic regulation of Polycomb complex activity orchestrated by JMJ balances self-renewal and differentiation, highlighting the involvement of chromatin dynamics in cell-fate transitions...
  87. ncbi Ring1-mediated ubiquitination of H2A restrains poised RNA polymerase II at bivalent genes in mouse ES cells
    Julie K Stock
    Nuclear Organisation, MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital Campus, Du Cane Road, London W12 0NN, UK
    Nat Cell Biol 9:1428-35. 2007
    ..These observations provide an insight into the molecular mechanisms that allow ES cells to self-renew and yet retain the ability to generate multiple lineage outcomes...
  88. ncbi Rett syndrome is caused by mutations in X-linked MECP2, encoding methyl-CpG-binding protein 2
    R E Amir
    Department of Pediatrics, Baylor College of Medicine, Houston, Texas 77030, USA
    Nat Genet 23:185-8. 1999
    ..This suggests that the mother is a germline mosaic for this mutation. Our study reports the first disease-causing mutations in RTT and points to abnormal epigenetic regulation as the mechanism underlying the pathogenesis of RTT...
  89. ncbi The transmembrane kinase Ire1p is a site-specific endonuclease that initiates mRNA splicing in the unfolded protein response
    C Sidrauski
    Department of Biochemistry and Biophysics, University of California, School of Medicine, San Francisco 94143 0448, USA
    Cell 90:1031-9. 1997
    ..The addition of purified tRNA ligase completes splicing; we therefore have reconstituted HAC1 mRNA splicing in vitro from purified components...
  90. pmc An early T cell lineage commitment checkpoint dependent on the transcription factor Bcl11b
    Long Li
    Division of Biology 156 29, California Institute of Technology, Pasadena, CA 91125, USA
    Science 329:89-93. 2010
    ....
  91. ncbi The histone H3 lysine-27 demethylase Jmjd3 links inflammation to inhibition of polycomb-mediated gene silencing
    Francesca De Santa
    Department of Experimental Oncology, European Institute of Oncology, Campus IFOM IEO, Via Adamello 16, 20139 Milan, Italy
    Cell 130:1083-94. 2007
    ....
  92. ncbi CAG expansions in a novel gene for Machado-Joseph disease at chromosome 14q32.1
    Y Kawaguchi
    Department of Pharmacology, Kyoto University Faculty of Medicine, Japan
    Nat Genet 8:221-8. 1994
    ..Southern blot analyses and genomic cloning demonstrates the existence of related genes. These results raise the possibility that similar abnormalities in related genes may give rise to diseases similar to MJD...
  93. ncbi Engineering stability in gene networks by autoregulation
    A Becskei
    EMBL, Structures and Biocomputing, Heidelberg, Germany
    Nature 405:590-3. 2000
    ..Here we have designed and constructed simple gene circuits consisting of a regulator and transcriptional repressor modules in Escherichia coli and we show the gain of stability produced by negative feedback...
  94. pmc The MuvB complex sequentially recruits B-Myb and FoxM1 to promote mitotic gene expression
    Subhashini Sadasivam
    Department of Medical Oncology, Dana Farber Cancer Institute, Boston, Massachusetts 02215, USA
    Genes Dev 26:474-89. 2012
    ..Our results reveal a novel role for the MuvB complex in recruiting B-Myb and FoxM1 to promote late cell cycle gene expression and in regulating cell cycle gene expression from quiescence through mitosis...
  95. pmc Short RNAs are transcribed from repressed polycomb target genes and interact with polycomb repressive complex-2
    Aditi Kanhere
    Division of Infection and Immunity, University College London, London W1T 4JF, UK
    Mol Cell 38:675-88. 2010
    ..We propose that short RNAs play a role in the association of PRC2 with its target genes...
  96. pmc A dual-kinase mechanism for Wnt co-receptor phosphorylation and activation
    Xin Zeng
    Neurobiology Program, Children s Hospital Boston, Department of Neurology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 438:873-7. 2005
    ....
  97. ncbi Establishment of histone h3 methylation on the inactive X chromosome requires transient recruitment of Eed-Enx1 polycomb group complexes
    Jose Silva
    X Inactivation Group, MRC Clinical Sciences Centre, ICSM, Hammersmith Hospital, Du Cane Road, London W12 0NN, United Kingdom
    Dev Cell 4:481-95. 2003
    ..Functional analysis demonstrates that Eed-Enx1 is required to establish methylation of histone H3 at lysine 9 and/or lysine 27 on Xi and that this, in turn, is required to stabilize the Xi chromatin structure...
  98. pmc CTCF physically links cohesin to chromatin
    Eric D Rubio
    Department of Radiation Oncology, University of Washington School of Medicine, Seattle WA 98195, USA
    Proc Natl Acad Sci U S A 105:8309-14. 2008
    ..These results have implications for the functional role of cohesin subunits in the pathogenesis of Cornelia de Lange syndrome and Roberts syndromes...
  99. pmc Maternal Rnf12/RLIM is required for imprinted X-chromosome inactivation in mice
    Jongdae Shin
    Program in Gene Function and Expression, University of Massachusetts Medical School UMMS, Worcester, Massachusetts 01605, USA
    Nature 467:977-81. 2010
    ..These results assign crucial functions to the maternal deposit of Rnf12/RLIM for the initiation of imprinted XCI...
  100. pmc The miR-200 family determines the epithelial phenotype of cancer cells by targeting the E-cadherin repressors ZEB1 and ZEB2
    Sun Mi Park
    The Ben May Department for Cancer Research, The University of Chicago, Chicago, Illinois 60637, USA
    Genes Dev 22:894-907. 2008
    ..Conversely, inhibition of miR-200 reduced E-cadherin expression, increased expression of Vimentin, and induced EMT. Our data identify miR-200 as a powerful marker and determining factor of the epithelial phenotype of cancer cells...
  101. ncbi Stochastic gene expression in a single cell
    Michael B Elowitz
    Laboratory of Cancer Biology, Center for Studies in Physics and Biology, Rockefeller University, New York, NY 10021, USA
    Science 297:1183-6. 2002
    ..These results establish a quantitative foundation for modeling noise in genetic networks and reveal how low intracellular copy numbers of molecules can fundamentally limit the precision of gene regulation...

Research Grants62

  1. Nuclear Events in PTH Action on Bone
    Nicola C Partridge; Fiscal Year: 2013
    ..the specific aims to test our hypothesis of this revised competing continuation proposal focus on the co-repressor proteins, HDAC4 and SIRT1, and will, 1) investigate the role of HDAC4 in regulating the expression of Runx2-dependent ..
  2. Mediator and epigenetic control of neuronal gene expression and differentiation
    Thomas G Boyer; Fiscal Year: 2013
    ..In this capacity, Mediator serves to channel regulatory signals from activator and repressor proteins to affect changes in gene expression programs that control diverse physiological processes, including cell ..
  3. REGULATION OF DEFENSE SIGNALING IN TOMATO
    Gregg A Howe; Fiscal Year: 2012
    ..model systems to develop the hypotheses that COI1 is a receptor for JA-Ile and coronatine, and that JAZ repressor proteins determine the specificity by which SCFCOI1 controls the diversity of jasmonate-regulated processes...
  4. Translational Regulation of Patterning in Drosophila
    Elizabeth R Gavis; Fiscal Year: 2013
    ..stem-loops that function in oogenesis and embryogenesis, respectively, through their interaction with the repressor proteins Glorund (Glo) and Smaug (Smg)...
  5. Targeting DNA Secondary Structures for Bcl-2 Gene Regulation
    Danzhou Yang; Fiscal Year: 2013
    ..a manner analogous to the genetic switch in the bacteriophage lambda controlled by the interactive Cro and Repressor proteins, whose operator regions (ORs) overlap with each other's promoter regions and thereby inhibit each other&..
  6. Critical functions specified by the MLL CXXC domain determine leukemogenic capaci
    NOAH WARREN BIRCH; Fiscal Year: 2012
    ..addition, we have previously shown that the MLL repression domain (containing the CXXC domain) binds to co-repressor proteins. Differential binding of these repressor proteins to MLL and MLL2 may affect overall function, but might ..
  7. The Role of Zinc Finger Genes in Pancreatic Cell Growth
    Raul A Urrutia; Fiscal Year: 2013
    ..the regulation of exocrine pancreatic cell growth by novel TGF[unreadable]-inducible KLF zinc finger repressor proteins discovered by our laboratory...
  8. Quantitative Proteomic Reconfiguration in Induction of Neuroprotection against St
    An Zhou; Fiscal Year: 2012
    ..This discovery implicates epigenetic gene repressor proteins (PcGs) as responsible for the process of transcription suppression resulting in ischemic tolerance...
  9. STRUCTURE AND REGULATION OF THE CD1 MULTIGENE FAMILY
    PAUL BLEICHER; Fiscal Year: 1992
    ..developmental stage specific and interferon inducible enhancers; (6) search for possible enhancer and repressor proteins using gel retardation and DNA footprinting analysis; (7) determine the exact chromosomal location of the CD1 ..
  10. SUBUNIT INTERACTIONS IN DNA BINDING PROTEINS
    Catherine Royer; Fiscal Year: 1992
    ..Two of these proteins are repressor proteins, the lac and trp repressors...
  11. IMMUNOLOGIC REGULATION OF PULMONARY FIBROSIS
    Robert Mason; Fiscal Year: 2001
    ....
  12. Purchase of Analytical Ultracentrifuge for Molecular Biophysics at Florida State
    TIMOTHY LOGAN; Fiscal Year: 2007
    ..NIH funded projects that will be enhanced by this equipment include: metal activation of repressor proteins obtained from Corynebacterium diphtheriae, Mycobacterium tuberculosis, and Bacillus anthracis, the assembly ..
  13. GENETIC REGULATORY MECHANISMS IN NEUROSPORA
    Mary Case; Fiscal Year: 1990
    ..3) To isolate significant amounts of both activator and repressor proteins by cloning the qa-1F and qa-1S genes into high expression vectors...
  14. TRANSCRIPTIONAL REGULATION OF CARDIOMYOCYTE DEVELOPMENT
    Gordon Huggins; Fiscal Year: 2003
    ..molecular mechanisms by which GATA proteins, in conjunction with other transcriptionfactors and coactivator/ repressor proteins regulate cardiogenesis and cardiac hypertrophy...
  15. In Vitro Reconstitution of Alpha Fetroprotein Regulation
    Michelle Barton; Fiscal Year: 2007
    ..to loss of AFP transcription repression by facilitating alterations of chromatin structure and depletion of repressor proteins. Our experiments will take advantage of in vitro chromatin and synthetic nuclei transcription systems used ..
  16. HTS for Androgen Receptor Antagonists that Recruit NCoR
    Steven Balk; Fiscal Year: 2006
    ..We have shown that AR can interact with co-repressor proteins, including NCoR and SMRT, and that this interaction is enhanced by mifepristone (RU486), demonstrating that ..
  17. AKT MEDIATED TAMOXIFEN RESISTANCE IN BREAST CANCER CELLS
    Harikrishna Nakshatri; Fiscal Year: 2005
    The estrogen receptor alpha (ERalpha) enhances or represses transcription by recruiting co-activator or co-repressor proteins to regulatory elements in the target genes...
  18. HoxA10 Function During Myeloid Differentiation
    Elizabeth Eklund; Fiscal Year: 2006
    ..In vitro binding assays indicate that HoxA10 recruits transcriptional co-repressor proteins to the CYBB and NCF2 promoters...
  19. MAINTAINANCE OF HOMEOTIC GENE TRANSCRIPTION
    Mark Biggin; Fiscal Year: 2002
    ..We will test whether PcG repressor proteins or general transcription factors directly interact with minimal zeste repression domains...
  20. IDENTIFICATION AND ANALYSIS OF HAT/COACTIVATOR COMPLEXES
    Patrick Grant; Fiscal Year: 2005
    ..regulation of gene expression from chromatin requires the concerted action of gene specific activator and repressor proteins, general transcription factors and chromatin modifying activities...
  21. ESTROGEN ACTION IN THE PITUITARY
    Margaret Shupnik; Fiscal Year: 2005
    ..distinct pathways of ligand- independent ER stimulation, and differential expression of ER coactivator and repressor proteins. We previously identified a pituitary-specific truncated ERalpha in rat pituitary cells (TERP)...
  22. NUCLEAR RECEPTORS 2000
    Ronald Evans; Fiscal Year: 2000
    ..Pathways for steroid hormone action, cell-cycle control, co-activator and co-repressor proteins, oncogenic activation, tumor suppression, and medical therapeutics have all been dramatically elucidated ..
  23. BLOCKING HEPARANASE TRANSCRIPTION TO CONTROL METASTASIS
    Hong Qi; Fiscal Year: 2000
    ..we propose to use recent advances in zinc finger DNA binding protein design to create novel transcription repressor proteins which are capable of repressing heparanase gene expression in vivo...
  24. ROLE OF CHROMATIN REMODELING IN GROWTH CONTROL
    GAVIN SCHNITZLER; Fiscal Year: 2004
    ..chromatin remodeling complexes and enzymes that acetylate the histone tails, and enhanced by complexes of repressor proteins, histone deacetylases, and other factors...
  25. Conference on Tissue-Selective Nuclear Receptors
    Donald McDonnell; Fiscal Year: 2004
    ..There is only scant understanding of the mechanisms of tissue selectivity. Co-activator and co-repressor proteins are thought to contribute, but little is known about how complex temporal patterns of gene expression exist ..
  26. EPIGENETIC MODIFICATIONS OF GABA NEURONS IN PSYCHOSIS
    Rajiv Sharma; Fiscal Year: 2009
    ..We will apply antisense oligonucleotide strategies to down regulate epigenetic repressor proteins such as DNMT1 and MeCP2 to dissect their contribution to depolarization induced gene transcription...
  27. SPATIAL REGULATION OF DEVELOPMENTAL GENE EXPRESSION
    Ronald Evans; Fiscal Year: 2009
    ..is that the transcriptional regulation by retinoids is mediated through distinct co-activator and co-repressor proteins that interact with nuclear receptors in a hormone-dependent fashion...
  28. ULTRA HIGH FIELD NMR SPECTROMETER
    LEONARD SPICER; Fiscal Year: 1990
    ..Research in the following areas of biomedical science will be significantly advanced: 1. Repressor proteins and DNA regulation 2. Metalloenzyme structure and function 3. Protein folding 4...
  29. PPARs: Transcriptional Regulators of Metabolism
    Ronald Evans; Fiscal Year: 2003
    ..4) Identifying new coactivator/repressor proteins and in particular, determining which of these components are associated with specific biological programs...
  30. Conference on the PPARs
    Bruce Spiegelman; Fiscal Year: 2001
    ..3) Defining long-term benefits and risks of PPAR active drugs. (4) Identifying new coactivator/repressor proteins and, in particular, determining which of these components are associated with specific biological programs...
  31. TRANSCRIPTION REPRESSORS TO ANALYZE CANCER GENES
    Edward Rebar; Fiscal Year: 2000
    ..In this proposal, we will use rationally-designed zinc finger proteins (ZFPs) to target repressor proteins to the promoter regions of a tumor suppressor protein (p19ARF) and a human oncogene (cyclin D1) (phase I) ..
  32. SOLUTION STRUCTURE AND FUNCTION OF MUTANT CRO REPRESSORS
    Brian Reid; Fiscal Year: 1990
    ..This will lead to the design and engineering of repressor proteins capable of binding to any pre-determined DNA sequences...
  33. Iron and O2 in the Regulation of P. aeruginosa Virulence
    Michael Vasil; Fiscal Year: 2003
    ..in a major way is dealt with in a diverse array of pathogenic and commensal prokaryotic microbes, by repressor proteins, which play the key role in controlling iron homeostasis at the level of transcription...
  34. STEROIDS REPRESS LH THROUGH PROTEIN/PROTEIN INTERACTIONS
    JOAN JORGENSEN; Fiscal Year: 2003
    ..In Aim 1, the yeast two-hybrid approach will be used in both a directed and random fashion to screen for co-repressor proteins. In addition, we postulate that at least one phosphorylated residue of the AR is required for successful ..
  35. REPRESSOR SENSITIVE MUTATIONS IN DROSOPHILA
    FRANK LASKI; Fiscal Year: 2001
    ..We will initially make a rs mutation in the gene hedgehog. We will then test a number of repressor proteins to determine which one has the strongest repressor activity...
  36. DYSREGULATION OF MMP 9 DURING SKIN CARCINOGENESIS
    JOYCE RUNDHAUG; Fiscal Year: 2002
    ..will also be done in the non-malignant ells, if necessary, to determine if the lack of MMP-9 expression in these cells is due to a lack of positive transactivating factors rather than the presence of repressor proteins.
  37. Purkinje Cell Development: A Numerical Study
    Karl Herrup; Fiscal Year: 2005
    ..We will subdivide the 3.2kb region to more precisely identify the Purkinje cell enhancer. BAC transgenic mice will be used to identify the cis-element responsible for the banding. ..
  38. Neuron Target Interaction - a Developmental Genetic Study
    Karl Herrup; Fiscal Year: 2010
    ....
  39. Cell cycle events: outcomes measures in Alzheimer models
    Karl Herrup; Fiscal Year: 2010
    ....
  40. REGULATION OF PYRIMIDINE GENE EXPRESSION IN BACTERIA
    CHARLES TURNBOUGH; Fiscal Year: 2001
    ..Although these studies are done with E. coli, the work is likely to be applicable to the study of gene expression and regulation in all bacteria and probably in eukaryotes, as well. ..
  41. PROTEIN KINASE NETWORKS AND SPORULATION IN S. CEREVISIAE
    EDWARD P WINTER; Fiscal Year: 2010
    ..These studies will provide mechanistic insights into how protein kinase networks control developmental programs in general and meiotic development in particular. ..
  42. Development of Sensory maps in the Mammalian neocortex
    Yasushi Nakagawa; Fiscal Year: 2010
    ..abstract_text> ..
  43. Analysis of the COP9 signalosome for Retinoblastoma function
    David N Arnosti; Fiscal Year: 2010
    ....
  44. Coactivation of Estrogen Receptor alpha/Sp1
    Stephen Safe; Fiscal Year: 2006
    ..abstract_text> ..
  45. STRUCTURE/FUNCTION OF CYCLIN DEPENDENT KINASES
    David Morgan; Fiscal Year: 2002
    ..The goal in this work will be to achieve a detailed biochemical understanding of the mechanisms by which this complex enzyme is assembled and regulated during mitosis. ..
  46. Epigenetic effects if endocrine disruptors in fetal germ cells
    PIROSKA E SZABO; Fiscal Year: 2010
    ....
  47. The Role of Zinc Finger Genes in Pancreatic Cell Growth
    Raul Urrutia; Fiscal Year: 2008
    ..underlying the regulation of exocrine pancreatic cell growth by novel TGF[unreadable] KLF zinc finger repressor proteins discovered by our laboratory...
  48. 2,3,7,8,--TCDD Effects on the Estrogenic Responses
    Stephen Safe; Fiscal Year: 2006
    ..The proposed Aims will address important mechanistic considerations on IDRE-independent inhibitory AhR-ERa crosstalk and pathways critical for inhibition of mammary tumor growth by SAhRMs. ..
  49. ALCOHOL & BRAIN DEVELOPMENT: MIDLINE CASCADE HYPOTHESIS
    Feng Zhou; Fiscal Year: 2003
    ....
  50. Mechanisms of Growth Factor-Estrogen Receptor Crosstalk
    Stephen Safe; Fiscal Year: 2006
    ..abstract_text> ..
  51. Molecular study of calcium channels in fungal pathogens.
    ANGELA GELLI; Fiscal Year: 2007
    ..abstract_text> ..
  52. DISSECTION OF THE FUNCTIONS OF HERPES SIMPLEX VIRUS ICPO
    Bernard Roizman; Fiscal Year: 2002
    ..The experimental design includes identification of the products of alternative splicing and characterization of the role of the ligands in infection. ..
  53. Role of Runx2 in Lung Cancer Progression
    Jitesh Pratap; Fiscal Year: 2008
    ..will enhance our understanding of requirement of stringent regulation of gene transcription and its perturbation in lung cancer cells that may be translated to novel therapies for lung cancer [unreadable] [unreadable] [unreadable]..
  54. Inhibition of Pancreatic Cancer by New PPARgamma Agonis*
    Stephen Safe; Fiscal Year: 2008
    ..PPAR? -active C-substituted DIMs that inhibit pancreatic cancer cell growth in vitro and in vivo, and these will serve as lead compounds for development of new drugs for clinical treatment of this devastating disease ..
  55. WNT signals in developing and postnatal teeth
    Sarah Millar; Fiscal Year: 2008
    ..These experiments will help to place WNT signals in the network of regulatory factors that control tooth development, and will test the potential use of WNT activation in strategies for tooth or enamel regeneration. ..
  56. FUNCTIONS FOR HERPES SIMPLEX VIRUS ICP22 AND US1.5
    Bernard Roizman; Fiscal Year: 2009
    ..In all instances a key question to be resolved is the contribution of each of these functions of ICP22 to the biology of the virus in the experimental animal host. ..
  57. Colon Cancer Inhibition by a Class of PPARgamma Agonists
    Stephen Safe; Fiscal Year: 2009
    ..The proposed studies will provide mechanistic insights on PPARy-dependent inhibition of colon cancer and identify compounds for future clinical studies. ..
  58. DNA damage response and DDB
    Pradip Raychaudhuri; Fiscal Year: 2009
    ..Does DDB play a role in the sustained activation of checkpoints following DNA damage? Does DNA damage-induced senescence depend upon DDB? 3. What are the in vivo functions of DDB1? ..
  59. Yersiniabactin and plague pathogenesis
    Robert Perry; Fiscal Year: 2008
    ..abstract_text> ..
  60. Epigenetics of fetal alcohol syndrome
    Feng Zhou; Fiscal Year: 2009
    ..The effect of these epigenetic key genes will be confirmed in the in vivo model exposed to alcohol vapor chamber with alcohol exposure pattern and paradigms similar to the whole embryonic culture. ..