serpins

Summary

Summary: A family of serine proteinase inhibitors which are similar in amino acid sequence and mechanism of inhibition, but differ in their specificity toward proteolytic enzymes. This family includes alpha 1-antitrypsin, angiotensinogen, ovalbumin, antiplasmin, alpha 1-antichymotrypsin, thyroxine-binding protein, complement 1 inactivators, antithrombin III, heparin cofactor II, plasminogen inactivators, gene Y protein, placental plasminogen activator inhibitor, and barley Z protein. Some members of the serpin family may be substrates rather than inhibitors of SERINE ENDOPEPTIDASES, and some serpins occur in plants where their function is not known.

Top Publications

  1. ncbi Pigment epithelium-derived factor: a potent inhibitor of angiogenesis
    D W Dawson
    Department of Microbiology Immunology, Department of Pathology, Robert H Lurie Comprehensive Cancer Center, Northwestern University Medical School, Chicago, IL 60611, USA
    Science 285:245-8. 1999
  2. ncbi Serpin structure, mechanism, and function
    Peter G W Gettins
    Department of Biochemistry and Molecular Genetics, University of Illinois at Chicago, M C 536, 1819 53 West Polk Street, Chicago, Illinois 60612, USA
    Chem Rev 102:4751-804. 2002
  3. ncbi Innate immune responses of a lepidopteran insect, Manduca sexta
    Michael R Kanost
    Department of Biochemistry, Kansas State University, Manhattan, KS 66506, USA
    Immunol Rev 198:97-105. 2004
  4. doi Circulating pigment epithelium-derived factor levels are associated with insulin resistance and decrease after weight loss
    Monica Sabater
    Department of Diabetes, Endocrinology, and Nutrition, Institut d Investigació Biomédica de Girona, Instituto de Salud Carlos III, Girona, Spain
    J Clin Endocrinol Metab 95:4720-8. 2010
  5. ncbi Role for DNA methylation in the control of cell type specific maspin expression
    Bernard W Futscher
    Department of Pharmacology and Toxicology, Bone Marrow Transplant Program, Arizona Cancer Center, The University of Arizona, Tucson, Arizona 85724, USA
    Nat Genet 31:175-9. 2002
  6. pmc Constitutive overexpression of pigment epithelium-derived factor inhibition of ocular melanoma growth and metastasis
    Hua Yang
    Department of Ophthalmology, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Invest Ophthalmol Vis Sci 51:28-34. 2010
  7. doi Vaspin serum concentrations in patients with carotid stenosis
    Gabriela Aust
    Center of Surgery, Department of Visceral, Transplantation, Thoracic, and Vascular Surgery, Medical Faculty, University of Leipzig, Leipzig, Germany
    Atherosclerosis 204:262-6. 2009
  8. ncbi The paradoxical expression of maspin in ovarian carcinoma
    Anil K Sood
    Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, University of Iowa, University of Iowa Hospitals and Clinics, Iowa City, Iowa 52242 1109, USA
    Clin Cancer Res 8:2924-32. 2002
  9. doi Conformational pathology of the serpins: themes, variations, and therapeutic strategies
    Bibek Gooptu
    School of Crystallography, Birkbeck College, University of London, London, UK
    Annu Rev Biochem 78:147-76. 2009
  10. doi Characterization of PEDF: a multi-functional serpin family protein
    S Filleur
    Department of Urology, Texas Tech University Health Sciences Center, 3601 4th Street, MS 6591, Lubbock, Texas 79430 6591, USA
    J Cell Biochem 106:769-75. 2009

Detail Information

Publications260 found, 100 shown here

  1. ncbi Pigment epithelium-derived factor: a potent inhibitor of angiogenesis
    D W Dawson
    Department of Microbiology Immunology, Department of Pathology, Robert H Lurie Comprehensive Cancer Center, Northwestern University Medical School, Chicago, IL 60611, USA
    Science 285:245-8. 1999
    ..These results suggest that PEDF may be of therapeutic use, especially in retinopathies where pathological neovascularization compromises vision and leads to blindness...
  2. ncbi Serpin structure, mechanism, and function
    Peter G W Gettins
    Department of Biochemistry and Molecular Genetics, University of Illinois at Chicago, M C 536, 1819 53 West Polk Street, Chicago, Illinois 60612, USA
    Chem Rev 102:4751-804. 2002
  3. ncbi Innate immune responses of a lepidopteran insect, Manduca sexta
    Michael R Kanost
    Department of Biochemistry, Kansas State University, Manhattan, KS 66506, USA
    Immunol Rev 198:97-105. 2004
    ..Inhibitory regulation of the proteinases by serpins and association of the proteinase homologs with a bacteria-binding lectin are important for ensuring a localized ..
  4. doi Circulating pigment epithelium-derived factor levels are associated with insulin resistance and decrease after weight loss
    Monica Sabater
    Department of Diabetes, Endocrinology, and Nutrition, Institut d Investigació Biomédica de Girona, Instituto de Salud Carlos III, Girona, Spain
    J Clin Endocrinol Metab 95:4720-8. 2010
    ..We aimed to study circulating pigment epithelium-derived factor (PEDF) in vivo in association with insulin resistance and in vitro in human adipocytes...
  5. ncbi Role for DNA methylation in the control of cell type specific maspin expression
    Bernard W Futscher
    Department of Pharmacology and Toxicology, Bone Marrow Transplant Program, Arizona Cancer Center, The University of Arizona, Tucson, Arizona 85724, USA
    Nat Genet 31:175-9. 2002
    ..These findings indicate that cytosine methylation is important in the establishment and maintenance of cell type restricted gene expression...
  6. pmc Constitutive overexpression of pigment epithelium-derived factor inhibition of ocular melanoma growth and metastasis
    Hua Yang
    Department of Ophthalmology, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Invest Ophthalmol Vis Sci 51:28-34. 2010
    ..This study investigates whether constitutive overexpression of PEDF inhibits the growth and hepatic micrometastasis of ocular melanoma...
  7. doi Vaspin serum concentrations in patients with carotid stenosis
    Gabriela Aust
    Center of Surgery, Department of Visceral, Transplantation, Thoracic, and Vascular Surgery, Medical Faculty, University of Leipzig, Leipzig, Germany
    Atherosclerosis 204:262-6. 2009
    ....
  8. ncbi The paradoxical expression of maspin in ovarian carcinoma
    Anil K Sood
    Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, University of Iowa, University of Iowa Hospitals and Clinics, Iowa City, Iowa 52242 1109, USA
    Clin Cancer Res 8:2924-32. 2002
    ..These paradoxical results may offer new insights regarding the role of maspin in ovarian cancer progression that may also impact diagnosis and treatment strategies...
  9. doi Conformational pathology of the serpins: themes, variations, and therapeutic strategies
    Bibek Gooptu
    School of Crystallography, Birkbeck College, University of London, London, UK
    Annu Rev Biochem 78:147-76. 2009
    ..Polymers of serpins can also form in extracellular tissues where they activate inflammatory cascades...
  10. doi Characterization of PEDF: a multi-functional serpin family protein
    S Filleur
    Department of Urology, Texas Tech University Health Sciences Center, 3601 4th Street, MS 6591, Lubbock, Texas 79430 6591, USA
    J Cell Biochem 106:769-75. 2009
    ....
  11. pmc Overexpression of pigment epithelium-derived factor decreases angiogenesis and inhibits the growth of human malignant melanoma cells in vivo
    Riichiro Abe
    Department of Dermatology, Hokkaido University Graduate School of Medicine, Sapporo, Japan
    Am J Pathol 164:1225-32. 2004
    ..PEDF therefore might be a promising novel therapeutic agent for treatment of patients with melanoma...
  12. pmc Interleukin-6 trans-signalling differentially regulates proliferation, migration, adhesion and maspin expression in human prostate cancer cells
    Frédéric R Santer
    Department of Urology, Innsbruck Medical University, Anichstrasse 35, A 6020 Innsbruck, Austria
    Endocr Relat Cancer 17:241-53. 2010
    ..Collectively, these data suggest that selective targeting of IL-6TS might allow to refine the currently available experimental anti-IL-6 therapies against PC...
  13. ncbi Identification of a lipase-linked cell membrane receptor for pigment epithelium-derived factor
    Luigi Notari
    National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 281:38022-37. 2006
    ....
  14. pmc Identification of the antivasopermeability effect of pigment epithelium-derived factor and its active site
    Hua Liu
    Wilmer Ophthalmological Institute, Johns Hopkins Hospital, Baltimore, MD 21287 8984, USA
    Proc Natl Acad Sci U S A 101:6605-10. 2004
    ..Furthermore, PEDF may represent a superior therapeutic approach to sepsis-associated hypotension, nephrotic syndrome, and other sight-threatening and life-threatening diseases resulting from excessive vascular permeability...
  15. ncbi Maspin, a serpin with tumor-suppressing activity in human mammary epithelial cells
    Z Zou
    Division of Cancer Genetics, Dana Farber Cancer Institute, Boston, MA 02115
    Science 263:526-9. 1994
    ..Analysis of human breast cancer specimens revealed that loss of maspin expression occurred most frequently in advanced cancers. These results support the hypothesis that maspin functions as a tumor suppressor...
  16. pmc Visceral adipose tissue-derived serine protease inhibitor: a unique insulin-sensitizing adipocytokine in obesity
    Kazuyuki Hida
    Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine and Dentistry, Okayama 700 8558, Japan
    Proc Natl Acad Sci U S A 102:10610-5. 2005
    ..These findings indicate that vaspin exerts an insulin-sensitizing effect targeted toward WATs in states of obesity...
  17. ncbi Pigment epithelium-derived factor suppresses ischemia-induced retinal neovascularization and VEGF-induced migration and growth
    Elia J Duh
    Department of Ophthalmology, Johns Hopkins University School of Medicine, Maumenee 809, 600 N Wolfe Street, Baltimore, MD 21287, USA
    Invest Ophthalmol Vis Sci 43:821-9. 2002
    ....
  18. ncbi Serum vaspin concentrations in human obesity and type 2 diabetes
    Byung Soo Youn
    University of Leipzig, Department of Medicine, Ph Rosenthal Str 27, 04103 Leipzig, Germany
    Diabetes 57:372-7. 2008
    ..We have recently shown that vaspin mRNA expression in adipose tissue is related to parameters of obesity and glucose metabolism. However, the regulation of vaspin serum concentrations in human obesity and type 2 diabetes is unknown...
  19. doi Proinflammatory cytokines and angiogenic and anti-angiogenic factors in vitreous of patients with proliferative diabetic retinopathy and eales' disease
    Ponnalagu Murugeswari
    Department of Immunology, Dr G Venkataswamy Eye Research Institute, Aravind Medical Research Foundation, Tamilnadu, India
    Retina 28:817-24. 2008
    ....
  20. ncbi Pigment epithelium-derived factor (PEDF) is an endogenous antiinflammatory factor
    Sarah X Zhang
    Department of Medicine Endocrinology, Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA
    FASEB J 20:323-5. 2006
    ..These findings suggest that PEDF is a novel endogenous anti-inflammatory factor in the eye. The decrease of ocular PEDF levels may contribute to inflammation and vascular leakage in DR...
  21. pmc Pigment epithelium-derived factor inhibits neointimal hyperplasia after vascular injury by blocking NADPH oxidase-mediated reactive oxygen species generation
    Kazuo Nakamura
    Department of Medicine, Division of Cardio Vascular Medicine, Kurume University School of Medicine, 67 Asahi Machi, Kurume 830 0011, Japan
    Am J Pathol 170:2159-70. 2007
    ..Our present study suggests that substitution of PEDF may be a novel therapeutic strategy for restenosis after balloon angioplasty...
  22. ncbi PEDF: anti-angiogenic guardian of ocular function
    Noel Bouck
    Dept of Microbiology Immunology and Robert H Lurie Comprehensive Cancer Center, Northwestern University Medical School, 310 East Superior Street, Chicago, IL 60611, USA
    Trends Mol Med 8:330-4. 2002
    ..The molecular basis for this delicate control of endothelial cells is beginning to be understood and strategies to test the ability of PEDF to ameliorate or prevent vessel damage in the eye are developing rapidly...
  23. ncbi Manduca sexta serpin-4 and serpin-5 inhibit the prophenol oxidase activation pathway: cDNA cloning, protein expression, and characterization
    Youren Tong
    Department of Biochemistry, Kansas State University, Manhattan, Kansas 66506, USA
    J Biol Chem 280:14923-31. 2005
    ..To investigate the roles of protease inhibitors in regulating this pathway, we cloned cDNAs for two new serpins (serpin-4 and serpin-5) from the tobacco hornworm, Manduca sexta...
  24. ncbi Circadian rhythm of serum vaspin in healthy male volunteers: relation to meals
    Eunheui Jeong
    Department of Internal Medicine, Asan Medical Center, University of Ulsan College of Medicine, Pungnap 2 dong, Songpa Gu, Seoul 138 736, Korea
    J Clin Endocrinol Metab 95:1869-75. 2010
    ..Visceral adipose tissue-derived serine protease inhibitor (vaspin) is a novel adipokine with insulin-sensitizing effects. However, the physiological role for vaspin in human metabolic regulation remains to be established...
  25. doi In vitro and in vivo biological activity of PEDF against a range of tumors
    Matthew L Broadhead
    University of Melbourne, St Vincent s Hospital, Department of Orthopaedics and Surgery, L3, Daly Wing, 35 Victoria Pde, Fitzroy 3065, Vic, Australia
    Expert Opin Ther Targets 13:1429-38. 2009
    ..These direct and indirect effects of PEDF have been examined in vitro and in vivo for a range of malignancies...
  26. doi Distinct melanization pathways in the mosquito Aedes aegypti
    Zhen Zou
    Department of Entomology and the Institute for Integrative Genome Biology, University of California, Riverside, Riverside, CA 92521, USA
    Immunity 32:41-53. 2010
    ..activation pathways carried out by different modules of serine proteases and their specific inhibitors serpins. Immune melanization proteases (IMP-1 and IMP-2) and Serpin-1 mediate hemolymph prophenoloxidase cleavage and ..
  27. pmc Tissue-type plasminogen activator induces opening of the blood-brain barrier via the LDL receptor-related protein
    Manuel Yepes
    Department of Vascular Biology, Holland Laboratory, American Red Cross, Rockville, Maryland 20855, USA
    J Clin Invest 112:1533-40. 2003
    ....
  28. pmc Protective effect of perindopril on diabetic retinopathy is associated with decreased vascular endothelial growth factor-to-pigment epithelium-derived factor ratio: involvement of a mitochondria-reactive oxygen species pathway
    Zhi Zheng
    Department of Ophthalmology, First People s Hospital of Shanghai, Shanghai Jiaotong University, Shanghai, China
    Diabetes 58:954-64. 2009
    ....
  29. doi The ratio of VEGF/PEDF expression in bone marrow mesenchymal stem cells regulates neovascularization
    Wei Fan
    Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove Campus, Brisbane QLD 4059, Australia
    Differentiation 81:181-91. 2011
    ....
  30. pmc Anti-tumor effect of adenovirus-mediated gene transfer of pigment epithelium-derived factor on mouse B16-F10 melanoma
    Li Ping Yang
    State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Clinical Medicine School, Sichuan University, Chengdu, Sichuan, PR China
    J Exp Clin Cancer Res 28:75. 2009
    ..As a potent inhibitor of tumor angiogenesis, pigment epithelium-derived factor (PEDF) has recently been studied and used as an anticancer agent in several tumor models...
  31. pmc Serpins flex their muscle: I. Putting the clamps on proteolysis in diverse biological systems
    Gary A Silverman
    Department of Pediatrics and Cell Biology and Physiology, Children s Hospital of Pittsburgh and Magee Womens Hospital, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15201, USA
    J Biol Chem 285:24299-305. 2010
    b>Serpins compose the largest superfamily of peptidase inhibitors and are well known as regulators of hemostasis and thrombolysis...
  32. ncbi Focus on Molecules: Pigment epithelium-derived factor (PEDF)
    S Patricia Becerra
    NIH NEI, 7 Memorial Drive, Room 304, Building 7, Bethesda, MD 20892, USA
    Exp Eye Res 82:739-40. 2006
  33. ncbi Two functional epitopes of pigment epithelial-derived factor block angiogenesis and induce differentiation in prostate cancer
    Stephanie Filleur
    Department of Urology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA
    Cancer Res 65:5144-52. 2005
    ..Our results map active regions for the two PEDF functions, signaling via distinct receptors, identify candidate peptides, and provide their mechanism of action for future development of PEDF-based tumor therapies...
  34. ncbi Nuclear cytokine-activated IKKalpha controls prostate cancer metastasis by repressing Maspin
    Jun Li Luo
    Laboratory of Gene Regulation and Signal Transduction, Department of Pharmacology and Cancer Center, School of Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093 0723, USA
    Nature 446:690-4. 2007
    ..We propose that tumour-infiltrating RANKL-expressing cells lead to nuclear IKKalpha activation and inhibition of Maspin transcription, thereby promoting the metastatic phenotype...
  35. ncbi Pigment epithelium-derived factor protects cultured retinal pericytes from advanced glycation end product-induced injury through its antioxidative properties
    Sho ichi Yamagishi
    Division of Endocrinology and Metabolism, Department of Medicine, Kurume University School of Medicine, Kurume 830 0011, Japan
    Biochem Biophys Res Commun 296:877-82. 2002
    ..Our present study suggests that substitution of PEDF proteins may be a promising strategy in treatment of patients with early diabetic retinopathy...
  36. ncbi Pigment epithelium-derived factor is a substrate for matrix metalloproteinase type 2 and type 9: implications for downregulation in hypoxia
    Luigi Notari
    Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892 0607, USA
    Invest Ophthalmol Vis Sci 46:2736-47. 2005
    ..Because hypoxia and VEGF regulate matrix metalloproteinases (MMPs), their effects on PEDF proteolysis were explored...
  37. ncbi Antiangiogenic property of pigment epithelium-derived factor in hepatocellular carcinoma
    Kojiro Matsumoto
    First Department of Internal Medicine, Nagasaki University School of Medicine, Sakamoto, Japan
    Hepatology 40:252-9. 2004
    ..Reduction of serum PEDF concentration associated with the development of chronic liver diseases may contribute to the progression of HCC. In addition, gene therapy using PEDF may provide an efficient treatment for HCC...
  38. ncbi Pigment epithelium-derived factor downregulates vascular endothelial growth factor (VEGF) expression and inhibits VEGF-VEGF receptor 2 binding in diabetic retinopathy
    Sarah X Zhang
    Department of Medicine, Department of Cell Biology, University of Oklahoma Health Sciences Center, 941 Stanton L Young Blvd, Oklahoma City, OK, USA
    J Mol Endocrinol 37:1-12. 2006
    ..The decrease in PEDF levels in the retina is at least partially responsible for the increase in VEGF expression and subsequent vascular leakage and neovascularization in diabetes...
  39. ncbi Pigment epithelium-derived factor gene therapy inhibits human pancreatic cancer in mice
    Ryunosuke Hase
    Department of Surgical Oncology, Division of Cancer Medicine, Hokkaido University Graduate School of Medicine, Sapporo, Hokkaido, Japan
    Clin Cancer Res 11:8737-44. 2005
    ..The purpose of this study was to investigate whether PEDF gene is a potent tumor suppressor and a potential candidate for cancer gene therapy...
  40. pmc A therapeutic strategy for choroidal neovascularization based on recruitment of mesenchymal stem cells to the sites of lesions
    Hui Yuan Hou
    Department of Ophthalmology, Eye Institute of Chinese PLA, Xijing Hospital, Fourth Military Medical University, Xi an, China
    Mol Ther 18:1837-45. 2010
    ..These results suggest that MSCs contribute to CNV and could serve as delivery vehicles of antiangiogenic agents for the treatment of a range of CNV-associated diseases...
  41. ncbi Pigment epithelium-derived factor behaves like a noninhibitory serpin. Neurotrophic activity does not require the serpin reactive loop
    S P Becerra
    Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 270:25992-9. 1995
    ..Altogether our data indicate that PEDF belongs to the subgroup of noninhibitory serpins and that its N-terminal region confers a neurite-promoting activity to the protein...
  42. ncbi Biological functions of maspin
    Caleb M Bailey
    Department of Anatomy and Cell Biology, The Carver College of Medicine, University of Iowa, Iowa City, IA, USA
    J Cell Physiol 209:617-24. 2006
    ....
  43. pmc Three pairs of protease-serpin complexes cooperatively regulate the insect innate immune responses
    Rui Jiang
    National Research Laboratory of Defense Proteins, College of Pharmacy, Pusan National University, Busan 609 735, Korea
    J Biol Chem 284:35652-8. 2009
    b>Serpins are known to be necessary for the regulation of several serine protease cascades...
  44. ncbi Serine proteinase inhibitors in arthropod immunity
    M R Kanost
    Department of Biochemistry, Kansas State University, Manhattan 66506, USA
    Dev Comp Immunol 23:291-301. 1999
    ..Some may inhibit fungal or bacterial proteinases. Others probably have roles in regulating endogenous proteinases involved in coagulation, prophenol oxidase activation, or cytokine activation...
  45. pmc Protection of the liver against CCl4-induced injury by intramuscular electrotransfer of a kallistatin-encoding plasmid
    Yong Diao
    Institute of Molecular Medicine, Huaqiao University, Quanzhou 362021, Fujian Province, China
    World J Gastroenterol 17:111-7. 2011
    ..To investigate the effect of transgenic expression of kallistatin (Kal) on carbon tetrachloride (CCl(4))-induced liver injury by intramuscular (im) electrotransfer of a Kal-encoding plasmid formulated with poly-L-glutamate (PLG)...
  46. ncbi Viral inhibition of inflammation: cowpox virus encodes an inhibitor of the interleukin-1 beta converting enzyme
    C A Ray
    Department of Microbiology and Immunology, Duke University Medical Center, Durham, North Carolina 27710
    Cell 69:597-604. 1992
    ..These viral countermeasures to host defenses against infection may contribute significantly to the pathology associated with poxvirus infections...
  47. ncbi PEDF: a potential molecular therapeutic target with multiple anti-cancer activities
    Eugene T H Ek
    Department of Orthopaedics, University of Melbourne St Vincent s Hospital, Melbourne, PO Box 2900, Fitzroy, Melbourne, Australia
    Trends Mol Med 12:497-502. 2006
    ..We also discuss the prospects for PEDF therapy and the need for a closer evaluation of issues such as delivery, stability and potential toxicity...
  48. doi Anti-chondrosarcoma effects of PEDF mediated via molecules important to apoptosis, cell cycling, adhesion and invasion
    Mei Lin Tan
    Department of Orthopaedics, St Vincent s Hospital Melbourne, Vic, Australia
    Biochem Biophys Res Commun 398:613-8. 2010
    ..These findings seminally indicate that PEDF may have potential as an anti-cancer agent against chondrosarcoma...
  49. doi Central vaspin administration acutely reduces food intake and has sustained blood glucose-lowering effects
    N Kloting
    Department of Medicine, University of Leipzig, Liebigstr 20, 04103 Leipzig, Germany
    Diabetologia 54:1819-23. 2011
    ..Serum concentrations of vaspin show a food intake-related diurnal variation. We therefore tested the hypothesis that vaspin plays a role in the regulation of food intake...
  50. ncbi SERPINB3 induces epithelial-mesenchymal transition
    Santina Quarta
    Department of Clinical and Experimental Medicine, University of Padova, Italy
    J Pathol 221:343-56. 2010
    ....
  51. doi Pigment epithelium-derived factor associates with neuropathy and fibrosis in pancreatic cancer
    Tamar Samkharadze
    Department of General Surgery, Klinikum rechts der Isar, Technische Universitat Munchen, Munich, Germany
    Am J Gastroenterol 106:968-80. 2011
    ..Its role in pancreatic fibrosis and neuropathy is unknown...
  52. doi Polarized secretion of PEDF from human embryonic stem cell-derived RPE promotes retinal progenitor cell survival
    DanHong Zhu
    Department of Pathology, Keck School of Medicine, University of Southern California, Los Angeles, California 90089, USA
    Invest Ophthalmol Vis Sci 52:1573-85. 2011
    ..The purpose of this study was to determine the effect of polarization of hES-RPE monolayers on their ability to promote survival of RPCs...
  53. doi Pigment epithelium-derived factor gene therapy targeting retinal ganglion cell injuries: neuroprotection against loss of function in two animal models
    Masanori Miyazaki
    Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu University, Higashi ku, Fukuoka, Japan
    Hum Gene Ther 22:559-65. 2011
    ..These findings show that neuroprotective gene therapy using hPEDF can protect against retinal ganglion cell death, and support the potential feasibility of neuroprotective therapy for intractable glaucoma...
  54. ncbi SERPINB11 is a new noninhibitory intracellular serpin. Common single nucleotide polymorphisms in the scaffold impair conformational change
    David J Askew
    University of Pittsburgh Medical Center Newborn Medicine Program, Children s Hospital of Pittsburgh and Magee Womens Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    J Biol Chem 282:24948-60. 2007
    SERPINB11, the last of 13 human clade B serpins to be described, gave rise to seven different isoforms...
  55. ncbi Pigment epithelium-derived factor inhibits glioma cell growth in vitro and in vivo
    Tao Zhang
    Center of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, 12 Central Urumqi Road, Shanghai 200040, China
    Life Sci 81:1256-63. 2007
    ..PEDF activation can be a novel approach for future therapeutic purposes against gliomas...
  56. ncbi Aberrant expression of serpin squamous cell carcinoma antigen 2 in human tumor tissues and cell lines: evidence of protection from tumor necrosis factor-mediated apoptosis
    Atsushi Takeda
    Laboratory of Biochemistry, Faculty of Arts and Sciences, Sagami Women s University, Sagamihara shi, Kanagawa, Japan
    Biol Chem 383:1231-6. 2002
    ..Thus, SCCA2 overexpression in squamous tumor cells contributed to their survival by protecting them against TNF-alpha-induced apoptosis...
  57. ncbi Conformational properties of serine proteinase inhibitors (serpins) confer multiple pathophysiological roles
    S Janciauskiene
    Department of Medicine, Wallenberg Laboratory, Ing 46, Malmo University Hospital, S 20502, Malmo, Sweden
    Biochim Biophys Acta 1535:221-35. 2001
    Serine proteinase inhibitors (Serpins) are irreversible suicide inhibitors of proteases that regulate diverse physiological processes such as coagulation, fibrinolysis, complement activation, angiogenesis, apoptosis, inflammation, ..
  58. doi Pigment epithelium-derived factor inhibits advanced glycation end-products-induced cytotoxicity in retinal pericytes
    S Sheikpranbabu
    Department of Biotechnology, Division of Molecular and Cellular Biology, Kalasalingam University Kalasalingam Academy of Research and Education, Krishnankoil 626126, Anand Nagar, Tamilnadu, India
    Diabetes Metab 37:505-11. 2011
    ..This study investigated the effects of pigment epithelium-derived factor (PEDF) on advanced glycation end-product (AGE)-induced cytotoxicity in porcine retinal pericytes and the signalling mechanism involved...
  59. pmc Association between neuroserpin and molecular markers of brain damage in patients with acute ischemic stroke
    Raquel Rodríguez-González
    Clinical Neuroscience Research Laboratory, Department of Neurology, Hospital Clinico Universitario, University of Santiago de Compostela, Santiago de Compostela, Spain
    J Transl Med 9:58. 2011
    ..Our aim was to study whether neuroserpin serum levels could be associated to biomarkers of excitotoxicity, inflammation and blood brain barrier disruption...
  60. ncbi Association of maspin expression with the malignancy grade and tumor vascularization in breast cancer tissues
    T Hojo
    Growth Factor Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, 104-0045, Tokyo, Japan
    Cancer Lett 171:103-10. 2001
    ..These results suggest that maspin production in myoepithelial cells could downregulate the malignant phenotype of breast cancer...
  61. doi Short-term continuous subcutaneous insulin infusion decreases the plasma vaspin levels in patients with type 2 diabetes mellitus concomitant with improvement in insulin sensitivity
    Ke Li
    Department of Endocrinology, The Second Affiliated Hospital, Chongqing Medical University, 400016 Chongqing, China
    Eur J Endocrinol 164:905-10. 2011
    ..To investigate the effects of short-term continuous subcutaneous insulin infusion (CSII) on plasma vaspin levels in patients with newly diagnosed type 2 diabetes mellitus (T2DM)...
  62. ncbi Vitreous fluid of db/db mice exhibits alterations in angiogenic and metabolic factors consistent with early diabetic retinopathy
    Margo P Cohen
    Glycadia, Inc, Philadelphia, PA, USA
    Ophthalmic Res 40:5-9. 2008
    ....
  63. pmc Pigment epithelium-derived factor regulates lipid metabolism via adipose triglyceride lipase
    Melissa L Borg
    Biology of Lipid Metabolism Laboratory, Department of Physiology, Monash University, Clayton, Victoria, Australia
    Diabetes 60:1458-66. 2011
    ..We used ATGL(-/-) mice to determine the role of PEDF in regulating lipid and glucose metabolism...
  64. pmc Nicotine increases the VEGF/PEDF ratio in retinal pigment epithelium: a possible mechanism for CNV in passive smokers with AMD
    Marianne Pons
    Bascom Palmer Eye Institute, Department of Ophthalmology, University of Miami Miller School of Medicine, Miami, Florida 33136, USA
    Invest Ophthalmol Vis Sci 52:3842-53. 2011
    ....
  65. pmc Serpins flex their muscle: II. Structural insights into target peptidase recognition, polymerization, and transport functions
    James C Whisstock
    From the Department of Biochemistry and Molecular Biology and ARC Centre of Excellence in Structural and Functional Microbial Genomics, Monash University, Clayton, Victoria 3800, Australia
    J Biol Chem 285:24307-12. 2010
    Inhibitory serpins are metastable proteins that undergo a substantial conformational rearrangement to covalently trap target peptidases...
  66. doi Serum vaspin concentrations are closely related to insulin resistance, and rs77060950 at SERPINA12 genetically defines distinct group with higher serum levels in Japanese population
    Sanae Teshigawara
    Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2 5 1 Shikata cho, Kita ku, Okayama 700 8558, Japan
    J Clin Endocrinol Metab 97:E1202-7. 2012
    ..Vaspin is an adipokine with insulin-sensitizing effects identified from visceral adipose tissues of genetically obese rats...
  67. doi Serum levels of vaspin and visfatin in patients with coronary artery disease-Kozani study
    Nikolaos P E Kadoglou
    Department of Cardiology, Mamatsio General Hospital, Kozani, Greece
    Clin Chim Acta 412:48-52. 2011
    ..The present study aimed to investigate the relationship of those adipokines with the existence as well as the extent of coronary artery disease (CAD), suggesting a link between adiposity and atherosclerosis...
  68. ncbi Suppression of a squamous cell carcinoma (SCC)-related serpin, SCC antigen, inhibits tumor growth with increased intratumor infiltration of natural killer cells
    Y Suminami
    Department of Obstetrics and Gynecology, Yamaguchi University School of Medicine, Ube, Japan
    Cancer Res 61:1776-80. 2001
    ..These results suggest that antisense SCCA may suppress the growth of SCC in vivo not only by the augmentation of intracellular apoptosis but also by the increased infiltration of NK cells into the tumor...
  69. pmc Alterations of retinal pigment epithelium cause AMD-like retinopathy in senescence-accelerated OXYS rats
    Anton M Markovets
    Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia
    Aging (Albany NY) 3:44-54. 2011
    ..These data suggest that primary cellular degenerative alterations in the RPE cells secondarily lead to choriocapillaris atrophy and results in complete loss of photoreceptor cells in the OXYS rats' retina by the age of 24 months...
  70. ncbi PEDF: a multifaceted neurotrophic factor
    Joyce Tombran-Tink
    Division of Pharmaceutical Sciences, University of Missouri Kansas City, 5005 Rockhill Road, Kansas City, Missouri 64110, USA
    Nat Rev Neurosci 4:628-36. 2003
  71. pmc Overexpression of pigment epithelium-derived factor inhibits retinal inflammation and neovascularization
    Kyoungmin Park
    Department of Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA
    Am J Pathol 178:688-98. 2011
    ..The PEDF-Tg mice provide a useful model for studying the roles of angiogenic inhibitors in neovascular disorders such as diabetic retinopathy...
  72. ncbi Wilms' tumor growth is suppressed by antiangiogenic pigment epithelium-derived factor in a xenograft model
    Lisa P Abramson
    Division of Pediatric Surgery, Children s Memorial Hospital, and the Department of Pathology, Northwestern University Medical School, Chicago, Illinois 60611, USA
    J Pediatr Surg 38:336-42; discussion 336-42. 2003
    ..The authors postulated that systemic administration of PEDF would decrease Wilms' tumor growth in a xenograft model, and increased renal vascularity would result in a mouse null for PEDF...
  73. ncbi Pigment epithelium-derived factor (PEDF) and vascular endothelial growth factor (VEGF) in aged human choroid and eyes with age-related macular degeneration
    Imran A Bhutto
    Department of Ophthalmology, The Wilmer Ophthalmological Institute, Johns Hopkins Hospital, Johns Hopkins University School of Medicine, 600 North Wolfe Street, Baltimore, MD 21287 9115, USA
    Exp Eye Res 82:99-110. 2006
    ..The decrease in PEDF may disrupt the balance and be permissive for the formation of choroidal neovascularization (CNV) in AMD...
  74. pmc Increased serum pigment epithelium derived factor levels in Type 2 diabetes patients
    Alicia Jenkins
    Department of Medicine, Endocrinology, Harold Hamm Oklahoma Diabetes Center, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    Diabetes Res Clin Pract 82:e5-7. 2008
    ..3 (2.8) vs. 3.2 (2.0)mug/ml, p<0.001. In diabetes, PEDF correlated with BMI, serum creatinine and LDL-cholesterol, but not with other lipids, HbA1c or CRP. PEDF did not differ by drugs, complications, or gender...
  75. doi STAT6 activation by Toxoplasma gondii infection induces the expression of Th2 C-C chemokine ligands and B clade serine protease inhibitors in macrophage
    Hye Jin Ahn
    Department of Parasitology and the Catholic Institute of Parasitic Diseases, College of Medicine, Catholic University of Korea, Seoul 137 701, South Korea
    Parasitol Res 105:1445-53. 2009
    ..It also mediated expression of two large clusters of C-C chemokine ligands (CCLs) and serine protease inhibitors (SERPINs) in microarray of T. gondii-infected macrophages...
  76. ncbi Pigment epithelium-derived factor inhibits angiogenesis via regulated intracellular proteolysis of vascular endothelial growth factor receptor 1
    Jun Cai
    School of Optometry and Vision Sciences, Cardiff University, UK
    J Biol Chem 281:3604-13. 2006
    ..This confirms the importance of PEDF and VEGFR-1 in the negative regulation of angiogenesis...
  77. ncbi Structure-function relationships of PEDF
    T Kawaguchi
    Department of Digestive Disease Information and Research and Department of Medicine, Kurume University School of Medicine, Kurume 830 0011, Japan
    Curr Mol Med 10:302-11. 2010
    ..In addition, since PEDF is recently identified as a regulator for glucose and lipid metabolism, we also discuss PEDF structures specially related to insulin-sensitizing and triglyceridereducing properties...
  78. ncbi Granzyme M is a regulatory protease that inactivates proteinase inhibitor 9, an endogenous inhibitor of granzyme B
    Sami Mahrus
    Chemistry and Chemical Biology Graduate Program, University of California, San Francisco, California 94143 2280, USA
    J Biol Chem 279:54275-82. 2004
    ..Finally, the interaction between granzyme M and the serpins alpha(1)-antichymotrypsin, alpha(1)-proteinase inhibitor, and proteinase inhibitor 9 was characterized by using a ..
  79. ncbi Expression of maspin predicts poor prognosis in breast-cancer patients
    Yoshihisa Umekita
    Department of Pathology, Faculty of Medicine, Kagoshima University, Sakuragaoka, Kagoshima, Japan
    Int J Cancer 100:452-5. 2002
    ....
  80. pmc Probing neuroserpin polymerization and interaction with amyloid-beta peptides using single molecule fluorescence
    Albert Chiou
    Department of Chemistry, University of Cambridge, Cambridge, United Kingdom
    Biophys J 97:2306-15. 2009
    ..The A beta(1-40) is displaced from the complex so that it acts as a catalyst and is not incorporated into neuroserpin polymers...
  81. ncbi Expression of the p53 and Maspin protein in primary prostate cancer: correlation with clinical features
    S Machtens
    Department of Urology, Hannover University Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany
    Int J Cancer 95:337-42. 2001
    ..Our investigation delivers first hints for a p53-dependent regulatory pathway of the Maspin protein in human prostate cancer...
  82. ncbi Novel mechanism for age-related macular degeneration: an equilibrium shift between the angiogenesis factors VEGF and PEDF
    K Ohno-Matsui
    Department of Ophthalmology and Visual Science, Graduate School, Tokyo Medical and Dental University, Yushima, Bunkyo ku, Tokyo, Japan
    J Cell Physiol 189:323-33. 2001
    ..This equilibrium shift may be significant in promoting a pathological condition of RPE cells and contributing to choroidal neovascularization in age-related macular degeneration...
  83. ncbi Expression of the tumor suppressor gene Maspin in human pancreatic cancers
    N Maass
    Department of Gynecologic Oncology, University of Kiel, 24105 Kiel, Germany
    Clin Cancer Res 7:812-7. 2001
    ..In addition, the detection of maspin in pancreatic tumor tissues and its lack of expression in all normal pancreatic tissues suggests that maspin may be a useful marker of primary human pancreatic cancer...
  84. ncbi Blocking tumor growth, invasion, and metastasis by maspin in a syngeneic breast cancer model
    H Y Shi
    Baylor College of Medicine, Department of Molecular and Cellular Biology, Houston, Texas 77030, USA
    Cancer Res 61:6945-51. 2001
    ..Thus, maspin can block primary tumor growth as well as invasion and metastasis. These data support the concept that maspin has a strong protective role against tumor progression...
  85. ncbi Epigenetic silencing of maspin gene expression in human breast cancers
    F E Domann
    Free Radical and Radiation Biology Program, Department of Radiology, College of Medicine, University of Iowa and the Iowa Cancer Center, Iowa City, IA 52242, USA
    Int J Cancer 85:805-10. 2000
    ..Thus, aberrant cytosine methylation and heterochromatinization of the maspin promoter may silence maspin gene expression, thereby contributing to the progression of human mammary cancer...
  86. ncbi Vaspin gene expression in human adipose tissue: association with obesity and type 2 diabetes
    Nora Kloting
    University of Leipzig, Department of Internal Medicine III, 04103 Leipzig, Germany
    Biochem Biophys Res Commun 339:430-6. 2006
    ..In conclusion, our data indicate that induction of human vaspin mRNA expression in adipose tissue is regulated in a fat depot-specific manner and could be associated with parameters of obesity, insulin resistance, and glucose metabolism...
  87. doi Pigment epithelium-derived factor (PEDF) is one of the most abundant proteins secreted by human adipocytes and induces insulin resistance and inflammatory signaling in muscle and fat cells
    S Famulla
    Institute of Clinical Biochemistry and Pathobiochemistry, German Diabetes Center, Dusseldorf, Germany
    Int J Obes (Lond) 35:762-72. 2011
    ..The aim of this study was to investigate the regulation and autocrine function of PEDF in human adipocytes and to determine its paracrine effects on human skeletal muscle cells (hSkMC) and human smooth muscle cells (hSMC)...
  88. ncbi Pigment epithelium-derived factor delays the death of photoreceptors in mouse models of inherited retinal degenerations
    M Cayouette
    Faculte de Medecine, Centre de Recherche Universite Laval Robert Giffard, 2601, de la Canardière, Beauport, Quebec, G1J 2G3, Canada
    Neurobiol Dis 6:523-32. 1999
    ..We conclude that PEDF can act as a survival-promoting factor for photoreceptors in vivo and could potentially be useful for the treatment of photoreceptor diseases...
  89. ncbi Decreased pigment epithelium-derived factor expression in human breast cancer progression
    Jun Cai
    Cell and Molecular Biology Group, School of Optometry and Vision Sciences, Wales College of Medicine, Cardiff University, United Kingdom
    Clin Cancer Res 12:3510-7. 2006
    ..The aim of this study was to correlate the expression of pigment epithelium-derived factor (PEDF), a potent endogenous antiangiogenic molecule, with severity and prognosis in breast cancer...
  90. ncbi Pigment epithelium-derived factor inhibits oxidative stress-induced apoptosis and dysfunction of cultured retinal pericytes
    Shinjiro Amano
    Department of Internal Medicine III, Kurume University School of Medicine, 67 Asahi Machi, Kurume 830 0011, Japan
    Microvasc Res 69:45-55. 2005
    ..Our present study suggests that substitution of PEDF proteins might be a promising therapeutic strategy for treatment of patients with early diabetic retinopathy...
  91. pmc A serpin that regulates immune melanization in the respiratory system of Drosophila
    Huaping Tang
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    Dev Cell 15:617-26. 2008
    ..Such signaling between local and systemic immune responses could represent an alarm mechanism that prepares the host in case a pathogen breaches epithelial defenses to invade internal tissues...
  92. ncbi Pigment epithelium-derived factor inhibits TNF-alpha-induced interleukin-6 expression in endothelial cells by suppressing NADPH oxidase-mediated reactive oxygen species generation
    Sho ichi Yamagishi
    Department of Internal Medicine III, Kurume University School of Medicine, Kurume 830 0011, Japan
    J Mol Cell Cardiol 37:497-506. 2004
    ..Our present study suggests that PEDF may play an important role in the development and progression of atherosclerosis...
  93. ncbi Expression of the granzyme B inhibitor, protease inhibitor 9, by tumor cells in patients with non-Hodgkin and Hodgkin lymphoma: a novel protective mechanism for tumor cells to circumvent the immune system?
    Bellinda A Bladergroen
    Department of Pathology, Free University Medical Center, Amsterdam, The Netherlands
    Blood 99:232-7. 2002
    ..Expression of this inhibitor is yet another mechanism used by tumor cells to escape their elimination by cytotoxic lymphocytes...
  94. ncbi Identification of the neuroprotective molecular region of pigment epithelium-derived factor and its binding sites on motor neurons
    Masako M Bilak
    Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA
    J Neurosci 22:9378-86. 2002
    ..The data support the pharmacotherapeutic potential of PEDF as a neuroprotectant in human motor neuron degeneration...
  95. ncbi Protective immunity against Ixodes ricinus induced by a salivary serpin
    P P Prevot
    Institut de Biologie et de Médecine Moléculaires, Universite Libre de Bruxelles, rue des Professeurs Jeener et Brachet, 12, B 6041 Gosselies, Belgium
    Vaccine 25:3284-92. 2007
    ..It belongs to the superfamily of serpins and interferes with both haemostasis and the immune response of the host...
  96. pmc Corneal angiogenic privilege: angiogenic and antiangiogenic factors in corneal avascularity, vasculogenesis, and wound healing (an American Ophthalmological Society thesis)
    Dimitri T Azar
    Massachusetts Eye and Ear Infirmary and Schepens Eye Research Institute, Harvard Medical School, Boston, Massachusetts, USA
    Trans Am Ophthalmol Soc 104:264-302. 2006
    ..To determine the molecular basis of corneal avascularity during wound healing and determine the role of angiogenic and antiangiogenic factors in corneal vasculogenesis...
  97. doi Pigment epithelium-derived factor contributes to insulin resistance in obesity
    Seamus Crowe
    Department of Physiology, Monash University, Clayton, Victoria 3800, Australia
    Cell Metab 10:40-7. 2009
    ..Overall, these results identify a causal role for PEDF in obesity-induced insulin resistance...
  98. doi Anti-angiogenic pigment epithelium-derived factor regulates hepatocyte triglyceride content through adipose triglyceride lipase (ATGL)
    Chuhan Chung
    Department of Medicine, Section of Digestive Diseases, Yale University School of Medicine, New Haven, CT, USA
    J Hepatol 48:471-8. 2008
    ..We postulated that hepatocyte triglyceride metabolism was dependent on interactions between PEDF and ATGL, and loss of PEDF would impair mobilization of triglycerides in the liver...
  99. ncbi Prognostic significance of maspin in pancreatic ductal adenocarcinoma: tissue microarray analysis of 223 surgically resected cases
    Dengfeng Cao
    Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, MD 21231, USA
    Mod Pathol 20:570-8. 2007
    ....
  100. ncbi Decreased pigment epithelium-derived factor is associated with metastatic phenotype in human and rat prostate tumors
    Sofia Halin
    Department of Medical Biosciences, Umea University, Umea, Sweden
    Cancer Res 64:5664-71. 2004
    ..These observations indicate that loss of pigment epithelium-derived factor expression could be associated with the progression toward a metastatic phenotype in prostate cancer...
  101. ncbi Analysis of novel disease-related genes in bronchial asthma
    Noriko Yuyama
    Genox Research, Inc Kawasaki, Japan
    Cytokine 19:287-96. 2002
    ..In a complex disorder such as asthma, this combination of in vitro and in vivo genomic approaches is a powerful discriminatory method enabling identification of novel disease-related genes and their mechanisms of regulation...

Research Grants80

  1. Serpin Modulation of Inflammatory Vasculitis; Potential for Immunomodulatory Ther
    ALEXANDRA ROSE LUCAS; Fiscal Year: 2010
    ..Two innovative therapeutic approaches, based upon the infusion of serpins, one derived from a myxoma virus, Serp-1, and one mammalian serpin, neuroserpin (NSP), have been investigated in ..
  2. Serpin Modulation of Inflammatory Vasculitis; Potential for Immunomodulatory Ther
    Alexandra Lucas; Fiscal Year: 2009
    ..Two innovative therapeutic approaches, based upon the infusion of serpins, one derived from a myxoma virus, Serp-1, and one mammalian serpin, neuroserpin (NSP), have been investigated in ..
  3. A Role of Novel Serpin Maspinin Prostate Tumor/Bone Interaction
    Shijie Sheng; Fiscal Year: 2010
    ..point of view, the activity of maspin to target serine protease-like molecules may not be substituted by other serpins that have evolved to acquire higher target specificities...
  4. A Role of Novel Serpin Maspinin Prostate Tumor/Bone Interaction
    Shijie Sheng; Fiscal Year: 2009
    ..point of view, the activity of maspin to target serine protease-like molecules may not be substituted by other serpins that have evolved to acquire higher target specificities...
  5. MECHANISMS AND TREATMENT OF COPD PROGRESSION
    Robert Wise; Fiscal Year: 2007
    ..antitrypsin (A1AT) deficiency emphasizes the role of A1AT as an elastase inhibitor, serine protease inhibitors (Serpins), including A1 AT, have broader biological effects beyond their classical action as protease inhibitors...
  6. MECHANISMS AND TREATMENT OF COPD PROGRESSION
    Robert Wise; Fiscal Year: 2009
    ..antitrypsin (A1AT) deficiency emphasizes the role of A1AT as an elastase inhibitor, serine protease inhibitors (Serpins), including A1 AT, have broader biological effects beyond their classical action as protease inhibitors...
  7. MOLECULAR INTERACTIONS REGULATING SERPIN FUNCTION
    Daniel Lawrence; Fiscal Year: 2000
    ..Structurally, PAI-1 provides an excellent example of the general serpins ability to adopt multiple conformation states that include active, latent, oxidized and noninhibitory substrate ..
  8. MOLECULAR INTERACTIONS REGULATING SERPIN FUNCTION
    Daniel Lawrence; Fiscal Year: 2001
    ..Structurally, PAI-1 provides an excellent example of the general serpins ability to adopt multiple conformation states that include active, latent, oxidized and noninhibitory substrate ..
  9. MOLECULAR INTERACTIONS REGULATING SERPIN FUNCTION
    Daniel Lawrence; Fiscal Year: 1999
    ..Structurally, PAI-1 provides an excellent example of the general serpins ability to adopt multiple conformation states that include active, latent, oxidized and noninhibitory substrate ..
  10. C1 INHIBITOR GENE AND HEREDITARY ANGIONEUROTIC EDEMA
    Alvin Davis; Fiscal Year: 2001
    ..To compare and contrast the structure of C1INH with other serpins, recombinant truncated C1INH will be crystallized...
  11. C1 INHIBITOR GENE AND HEREDITARY ANGIONEUROTIC EDEMA
    Alvin Davis; Fiscal Year: 2003
    ..To compare and contrast the structure of C1INH with other serpins, recombinant truncated C1INH will be crystallized...
  12. C1 INHIBITOR GENE AND HEREDITARY ANGIONEUROTIC EDEMA
    Alvin Davis; Fiscal Year: 2002
    ..To compare and contrast the structure of C1INH with other serpins, recombinant truncated C1INH will be crystallized...
  13. C1 INHIBITOR GENE AND HEREDITARY ANGIONEUROTIC EDEMA
    Alvin Davis; Fiscal Year: 2000
    ..To compare and contrast the structure of C1INH with other serpins, recombinant truncated C1INH will be crystallized...
  14. C1 INHIBITOR GENE AND HEREDITARY ANGIONEUROTIC EDEMA
    Alvin Davis; Fiscal Year: 1999
    ..To compare and contrast the structure of C1INH with other serpins, recombinant truncated C1INH will be crystallized...
  15. C1 INHIBITOR GENE AND HEREDITARY ANGIONEUROTIC EDEMA
    Alvin Davis; Fiscal Year: 2007
    ..To compare and contrast the structure of C1INH with other serpins, recombinant truncated C1INH will be crystallized...
  16. BIOCHEMISTRY OF PROPHENOL OXIDASE ACTIVATING PROTEINASE
    Haobo Jiang; Fiscal Year: 2002
    ..The inhibition reactions of the proteinase by M. sexta serpins and Kunitz-type trypsin inhibitors will be investigated to understand the physiological regulation of the enzyme...
  17. BIOCHEMISTRY OF PROPHENOL OXIDASE ACTIVATING PROTEINASE
    Haobo Jiang; Fiscal Year: 2003
    ..The inhibition reactions of the proteinase by M. sexta serpins and Kunitz-type trypsin inhibitors will be investigated to understand the physiological regulation of the enzyme...
  18. BIOCHEMISTRY OF PROPHENOL OXIDASE ACTIVATING PROTEINASE
    Haobo Jiang; Fiscal Year: 2000
    ..The inhibition reactions of the proteinase by M. sexta serpins and Kunitz-type trypsin inhibitors will be investigated to understand the physiological regulation of the enzyme...
  19. BIOCHEMISTRY OF PROPHENOL OXIDASE ACTIVATING PROTEINASE
    Haobo Jiang; Fiscal Year: 2001
    ..The inhibition reactions of the proteinase by M. sexta serpins and Kunitz-type trypsin inhibitors will be investigated to understand the physiological regulation of the enzyme...
  20. BIOCHEMISTRY OF PROPHENOL OXIDASE ACTIVATING PROTEINASE
    Haobo Jiang; Fiscal Year: 1999
    ..The inhibition reactions of the proteinase by M. sexta serpins and Kunitz-type trypsin inhibitors will be investigated to understand the physiological regulation of the enzyme...
  21. BIOCHEMISTRY OF PROPHENOL OXIDASE ACTIVATING PROTEINASE
    Haobo Jiang; Fiscal Year: 2000
    ..The inhibition reactions of the proteinase by M. sexta serpins and Kunitz-type trypsin inhibitors will be investigated to understand the physiological regulation of the enzyme...
  22. MOLECULAR BASIS OF BLOOD COAGULATION REGULATION
    Steven T Olson; Fiscal Year: 2010
    ..Two serpins, anti-thrombin and protein Z-dependent protease inhibitor (ZPI), are known to inhibit procoagulant proteases in a ..
  23. GENE REGULATION OF MAMMALIAN DNA VIRUSES
    Richard Moyer; Fiscal Year: 1999
    ..proteins will be screened genetically by means of the yeast two-hybrid system, and biochemically by use of tagged serpins. The results of these assays will be linked to an extensive analysis of the behavior of knockout and site- ..
  24. GENE REGULATION OF MAMMALIAN DNA VIRUSES
    Richard Moyer; Fiscal Year: 2002
    ..They focus here on poxvirus-encoded proteinase inhibitors (serpins). Orthopoxviruses, including cowpox virus (CPV), encode three serpins: SPI-1, SPI-2/crmA and SPI-3...
  25. GENE REGULATION OF MAMMALIAN DNA VIRUSES
    Richard Moyer; Fiscal Year: 2003
    ..They focus here on poxvirus-encoded proteinase inhibitors (serpins). Orthopoxviruses, including cowpox virus (CPV), encode three serpins: SPI-1, SPI-2/crmA and SPI-3...
  26. GENE REGULATION OF MAMMALIAN DNA VIRUSES
    Richard Moyer; Fiscal Year: 2005
    ..They focus here on poxvirus-encoded proteinase inhibitors (serpins). Orthopoxviruses, including cowpox virus (CPV), encode three serpins: SPI-1, SPI-2/crmA and SPI-3...
  27. GENE REGULATION OF MAMMALIAN DNA VIRUSES
    Richard Moyer; Fiscal Year: 2001
    ..They focus here on poxvirus-encoded proteinase inhibitors (serpins). Orthopoxviruses, including cowpox virus (CPV), encode three serpins: SPI-1, SPI-2/crmA and SPI-3...
  28. GENE REGULATION OF MAMMALIAN DNA VIRUSES
    Richard Moyer; Fiscal Year: 2004
    ..They focus here on poxvirus-encoded proteinase inhibitors (serpins). Orthopoxviruses, including cowpox virus (CPV), encode three serpins: SPI-1, SPI-2/crmA and SPI-3...
  29. REGULATORS OF HUMAN NEUTROPHIL PROTEASES
    SURESH TYAGI; Fiscal Year: 1992
    ..To characterize the general role of the single thiol in other serpins, alpha1-ACT will be examined...
  30. Mechanisms of Hemostatic Protease Inhibition by Serpins
    Ingrid Verhamme; Fiscal Year: 2009
    The long-term goal is to define the molecular mechanisms of thrombin (T) inhibition by the serpins, heparin cofactor II (HCII) and plasminogen activator inhibitor-1 (PAI-1), implicated in arterial thrombosis...
  31. Mechanisms of Hemostatic Protease Inhibition by Serpins
    Ingrid Verhamme; Fiscal Year: 2006
    ..by applicant): The long-term goal is to define the molecular mechanisms of thrombin (T) inhibition by the serpins, heparin cofactor II (HCII) and plasminogen activator inhibitor-1 (PAI-1), implicated in arterial thrombosis...
  32. Mechanisms of Hemostatic Protease Inhibition by Serpins
    Ingrid Verhamme; Fiscal Year: 2007
    ..by applicant): The long-term goal is to define the molecular mechanisms of thrombin (T) inhibition by the serpins, heparin cofactor II (HCII) and plasminogen activator inhibitor-1 (PAI-1), implicated in arterial thrombosis...
  33. Mechanisms of Hemostatic Protease Inhibition by Serpins
    Ingrid Verhamme; Fiscal Year: 2009
    The long-term goal is to define the molecular mechanisms of thrombin (T) inhibition by the serpins, heparin cofactor II (HCII) and plasminogen activator inhibitor-1 (PAI-1), implicated in arterial thrombosis...
  34. Mechanisms of Hemostatic Protease Inhibition by Serpins
    Ingrid M Verhamme; Fiscal Year: 2010
    The long-term goal is to define the molecular mechanisms of thrombin (T) inhibition by the serpins, heparin cofactor II (HCII) and plasminogen activator inhibitor-1 (PAI-1), implicated in arterial thrombosis...
  35. Tyrosine Sulfation and Chemokine Receptor Function
    Kevin Moore; Fiscal Year: 2003
    ..of proteins are known to be tyrosine sulfated in man, including certain adhesion molecules, coagulation factors, serpins, extracellular matrix proteins, and hormones and some of these require sulfation for optimal function...
  36. Serine Proteinase Inhibitors (Serpins) in Tick Feeding and Physiology
    Albert Mulenga; Fiscal Year: 2009
    ..In humans, serpins are involved in regulation of important of proteolytic pathways that are essential to life such as blood ..
  37. Tyrosine Sulfation and Chemokine Receptor Function
    Kevin Moore; Fiscal Year: 2004
    ..of proteins are known to be tyrosine sulfated in man, including certain adhesion molecules, coagulation factors, serpins, extracellular matrix proteins, and hormones and some of these require sulfation for optimal function...
  38. Tyrosine Sulfation and Chemokine Receptor Function
    Kevin Moore; Fiscal Year: 2006
    ..of proteins are known to be tyrosine sulfated in man, including certain adhesion molecules, coagulation factors, serpins, extracellular matrix proteins, and hormones and some of these require sulfation for optimal function...
  39. Tyrosine Sulfation and Chemokine Receptor Function
    Kevin Moore; Fiscal Year: 2005
    ..of proteins are known to be tyrosine sulfated in man, including certain adhesion molecules, coagulation factors, serpins, extracellular matrix proteins, and hormones and some of these require sulfation for optimal function...
  40. Serine Proteinase Inhibitors (Serpins) in Tick Feeding and Physiology
    Albert Mulenga; Fiscal Year: 2009
    ..In humans, serpins are involved in regulation of important of proteolytic pathways that are essential to life such as blood ..
  41. Serpin Regulation of Coagulation Proteases
    ALIREZA REZAIE; Fiscal Year: 2009
    ..by applicant): The activity of coagulation proteases is regulated primarily by the serine protease inhibitors (serpins) in plasma...
  42. Serpin Regulation of Coagulation Proteases
    Alireza R Rezaie; Fiscal Year: 2010
    ..by applicant): The activity of coagulation proteases is regulated primarily by the serine protease inhibitors (serpins) in plasma...
  43. Regulatory functions of alpha 1-antichymotrypsin in inflammation and Alzheimer's
    Jaya Padmanabhan; Fiscal Year: 2010
    ..ACT) is an acute phase serum glycoprotein that belongs to a class of serine protease inhibitors named serpins and is an integral component of the amyloid plaques in Alzheimer's disease (AD) patients...
  44. SUBSTRATE SPECIFICITY AND REGULATION OF THROMBIN
    John Sheehan; Fiscal Year: 1993
    ..The effects of specific mutations on thrombin substrate specificity and the rate of thrombin inhibition by serpins will be determined by kinetic methods...
  45. Poxvirus proteins involved in virion binding, membrane fusion, & entry into cells
    Richard Moyer; Fiscal Year: 2007
    ..We focus here on poxvirus- encoded serine proteinase inhibitors (serpins). Orthopoxviruses, including cowpox virus (CPV), encode three serpins: SPI-1, SPl-2/crmA and SPI-3...
  46. PROTEINASE SYSTEMS IN INSECT HEMOLYMPH
    Michael Kanost; Fiscal Year: 2003
    ..4. Analyze the regulation of plasma proteinases by M. sexta serine protease inhibitors (serpins).
  47. PROTEINASE SYSTEMS IN INSECT HEMOLYMPH
    Michael Kanost; Fiscal Year: 2006
    ..4. Analyze the regulation of plasma proteinases by M. sexta serine protease inhibitors (serpins).
  48. Intracellular Serpin Regulation of Intestinal Cell Necrosis
    GARY SILVERMAN; Fiscal Year: 2009
    ..Extensive searches within the largest family of peptidase inhibitors, the serpins (e.g., antithrombin and 11-antitrypsin), failed to yield candidate regulatory genes...
  49. MOLECULAR MECHANISM OF THROMBIN INHIBITION
    Scott Cooper; Fiscal Year: 2003
    ..The objective of this research is to better understand the different mechanisms by which the serpins antithrombin (AT) and protein C inhibitor (PCI) inhibit free thrombin and thrombin bound to thrombomodulin (TM)...
  50. CELLULAR INTERACTIONS OF PLASMA PROTEINS
    Peter Walsh; Fiscal Year: 2000
    ..Project 3 (S.C. bock, Serpin Structure and the Development of Neutrophil-Resistant Serpins) will focus on the structure-activity relationships with a naturally occurring anticoagulant serpin, antithrombin ..
  51. CELLULAR INTERACTIONS OF PLASMA PROTEINS
    Peter Walsh; Fiscal Year: 2001
    ..Project 3 (S.C. bock, Serpin Structure and the Development of Neutrophil-Resistant Serpins) will focus on the structure-activity relationships with a naturally occurring anticoagulant serpin, antithrombin ..
  52. CELLULAR INTERACTIONS OF PLASMA PROTEINS
    Peter Walsh; Fiscal Year: 1999
    ..Project 3 (S.C. bock, Serpin Structure and the Development of Neutrophil-Resistant Serpins) will focus on the structure-activity relationships with a naturally occurring anticoagulant serpin, antithrombin ..
  53. CELLULAR INTERACTIONS OF PLASMA PROTEINS
    Peter Walsh; Fiscal Year: 1999
    ..Project 3 (S.C. bock, Serpin Structure and the Development of Neutrophil-Resistant Serpins) will focus on the structure-activity relationships with a naturally occurring anticoagulant serpin, antithrombin ..
  54. CELLULAR INTERACTIONS OF PLASMA PROTEINS
    Peter Walsh; Fiscal Year: 1999
    ..Project 3 (S.C. bock, Serpin Structure and the Development of Neutrophil-Resistant Serpins) will focus on the structure-activity relationships with a naturally occurring anticoagulant serpin, antithrombin ..
  55. LIMITING CELLULAR INJURY--THE ROLE OF CYTOSOLIC SERPINS
    GARY SILVERMAN; Fiscal Year: 2001
    ..Finally, these studies may establish the presence of a novel intracellular defense mechanism for safeguarding against the consequences (e.g., lysosomal injury and proteinase release) of an overwhelmed antioxidant defense system. ..
  56. REGULATION OF EXTRACELLULAR PROTEOLYSIS BY SERPINS
    DANIEL KNAUER; Fiscal Year: 2002
    ..This places PN1, FXIa, APP and the LRP in a common biochemical pathway that may be directly involved in Alzheimer's disease pathology. ..
  57. SERINE PROTEINASE INHIBITORS IN INSECTS
    Michael Kanost; Fiscal Year: 1990
    Human plasma contains a superfamily of serine proteinase inhibitors, the serpins, which are glycoproteins of Mr = 40,000- 60,000...
  58. REGULATION OF EXTRACELLULAR PROTEOLYSIS BY SERPINS
    DANIEL KNAUER; Fiscal Year: 2001
    ..This places PN1, FXIa, APP and the LRP in a common biochemical pathway that may be directly involved in Alzheimer's disease pathology. ..
  59. REGULATION OF EXTRACELLULAR PROTEOLYSIS BY SERPINS
    DANIEL KNAUER; Fiscal Year: 2004
    ..This places PN1, FXIa, APP and the LRP in a common biochemical pathway that may be directly involved in Alzheimer's disease pathology. ..
  60. REGULATION OF EXTRACELLULAR PROTEOLYSIS BY SERPINS
    DANIEL KNAUER; Fiscal Year: 2003
    ..This places PN1, FXIa, APP and the LRP in a common biochemical pathway that may be directly involved in Alzheimer's disease pathology. ..
  61. Anti-inflammatory Serpin(AAT and Elafin) Gene Transfers*
    Sihong Song; Fiscal Year: 2002
    ..Lines of evidence suggest that serpins, namely AAT and elafin, may play important roles in protecting islets from autoimmunity and alloimmnunity...
  62. Anti-inflammatory Serpin(AAT and Elafin) Gene Transfers*
    Sihong Song; Fiscal Year: 2003
    ..Lines of evidence suggest that serpins, namely AAT and elafin, may play important roles in protecting islets from autoimmunity and alloimmnunity...
  63. RECOMBINANT SERPINS AND NEUTROPHILS IN ACUTE LUNG INJURY
    Michael Plotnick; Fiscal Year: 2000
    ..to our understanding of the biochemical properties and biological functions of serine protease inhibitor (serpins) as well as provide me with a foundation for a career as an independent investigator in the molecular and ..
  64. RECOMBINANT SERPINS AND NEUTROPHILS IN ACUTE LUNG INJURY
    Michael Plotnick; Fiscal Year: 1999
    ..to our understanding of the biochemical properties and biological functions of serine protease inhibitor (serpins) as well as provide me with a foundation for a career as an independent investigator in the molecular and ..
  65. STRUCTURE AND FUNCTION OF HEPARIN COFACTOR II
    DOUGLAS TOLLEFSEN; Fiscal Year: 1999
    ..In the second Specific Aim, peptides which mimic the reactive site loop present in all serpins will be evaluated with respect to their ability to influence HCII inhibitory functions...
  66. STRUCTURE AND FUNCTION OF HEPARIN COFACTOR II
    DOUGLAS TOLLEFSEN; Fiscal Year: 2000
    ..In the second Specific Aim, peptides which mimic the reactive site loop present in all serpins will be evaluated with respect to their ability to influence HCII inhibitory functions...
  67. Genetic analysis of heparan sulfate in vascular biology
    Jeffrey Esko; Fiscal Year: 2003
    ..with particular emphasis on heparin-binding proteinases (Protein C and tissue plasminogen activator) and serpins (antithrombin, tissue factor pathway inhibitor, and plasminogen activator inhibitor-I)...
  68. SERINE PROTEASE INHIBITORS & MECHANISMS OF INFLAMATION
    Harvey Rubin; Fiscal Year: 2001
    ..We are studying a family of high molecular weight plasma and cellular proteins, the serpins--serine protease inhibitors, some of which have evolved the remarkable property of inhibiting these proteases ..
  69. SERINE PROTEASE INHIBITORS & MECHANISMS OF INFLAMATION
    Harvey Rubin; Fiscal Year: 1999
    ..We are studying a family of high molecular weight plasma and cellular proteins, the serpins--serine protease inhibitors, some of which have evolved the remarkable property of inhibiting these proteases ..
  70. SERINE PROTEASE INHIBITORS & MECHANISMS OF INFLAMATION
    Harvey Rubin; Fiscal Year: 2000
    ..We are studying a family of high molecular weight plasma and cellular proteins, the serpins--serine protease inhibitors, some of which have evolved the remarkable property of inhibiting these proteases ..
  71. SERINE PROTEASE INHIBITORS & MECHANISMS OF INFLAMATION
    Harvey Rubin; Fiscal Year: 2002
    ..We are studying a family of high molecular weight plasma and cellular proteins, the serpins--serine protease inhibitors, some of which have evolved the remarkable property of inhibiting these proteases ..
  72. SERINE PROTEASE INHIBITORS & MECHANISMS OF INFLAMATION
    Harvey Rubin; Fiscal Year: 2004
    ..We are studying a family of high molecular weight plasma and cellular proteins, the serpins--serine protease inhibitors, some of which have evolved the remarkable property of inhibiting these proteases ..
  73. SERPIN STRUCTURE AND THE DEVELOPMENT OF NEUTROPHIL RESISTANT SERPINS
    Susan Bock; Fiscal Year: 1999
    ..Aim 3 is to make oxidation-resistant antielastase and anti-catG serpins that bind heparin...
  74. SERPIN STRUCTURE AND THE DEVELOPMENT OF NEUTROPHIL RESISTANT SERPINS
    Susan Bock; Fiscal Year: 2001
    ..Aim 3 is to make oxidation-resistant antielastase and anti-catG serpins that bind heparin...
  75. SERPIN STRUCTURE AND THE DEVELOPMENT OF NEUTROPHIL RESISTANT SERPINS
    Susan Bock; Fiscal Year: 1999
    ..Aim 3 is to make oxidation-resistant antielastase and anti-catG serpins that bind heparin...