- Genetic variation in IL28B predicts hepatitis C treatment-induced viral clearance
Institute for Genome Sciences and Policy, Center for Human Genome Variation, Duke University, Durham, North Carolina 27708, USA
Nature 461:399-401. 2009
- Protein production by auto-induction in high density shaking cultures
F William Studier
Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA
Protein Expr Purif 41:207-34. 2005
..Selenomethionine labeling was equally efficient in the commonly used methionine auxotroph B834(DE3) (found to be metE) or the prototroph BL21(DE3)...
- Boceprevir for untreated chronic HCV genotype 1 infection
Cedars Sinai Medical Center, Los Angeles, CA 90048, USA
N Engl J Med 364:1195-206. 2011
..The rate of sustained virologic response has been below 50% in cases of HCV genotype 1 infection. Boceprevir, a potent oral HCV-protease inhibitor, has been evaluated as an additional treatment in phase 1 and phase 2 studies...
- Telaprevir for previously untreated chronic hepatitis C virus infection
Ira M Jacobson
Weill Cornell Medical College and Center for the Study of Hepatitis C, New York, NY 10021, USA
N Engl J Med 364:2405-16. 2011
- Peginterferon alfa-2a plus ribavirin for chronic hepatitis C virus infection
Michael W Fried
University of North Carolina, Chapel Hill 27599, USA
N Engl J Med 347:975-82. 2002
..We compared the efficacy and safety of peginterferon alfa-2a plus ribavirin, interferon alfa-2b plus ribavirin, and peginterferon alfa-2a alone in the initial treatment of chronic hepatitis C...
- Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein
Nathan C Shaner
Nat Biotechnol 22:1567-72. 2004
..Three monomers with distinguishable hues from yellow-orange to red-orange have higher quantum efficiencies...
- Peginterferon-alpha2a and ribavirin combination therapy in chronic hepatitis C: a randomized study of treatment duration and ribavirin dose
Stephanos J Hadziyannis
Department of Medicine and Hepatology, Henry Dunant Hospital, Athens, Greece
Ann Intern Med 140:346-55. 2004
..Treatment with pegylated interferon (peginterferon) and ribavirin for 48 weeks is more effective than conventional interferon and ribavirin in patients with chronic hepatitis C...
- Boceprevir for previously treated chronic HCV genotype 1 infection
Bruce R Bacon
Saint Louis University School of Medicine, St Louis, Mo 63110 0250, USA
N Engl J Med 364:1207-17. 2011
..Boceprevir, a protease inhibitor that binds to the HCV nonstructural 3 (NS3) active site, has been suggested as an additional treatment...
- Telaprevir for retreatment of HCV infection
Johann Wolfgang Goethe University Medical Center, Frankfurt am Main, Germany
N Engl J Med 364:2417-28. 2011
..Up to 60% of patients with hepatitis C virus (HCV) genotype 1 infection do not have a sustained virologic response to therapy with peginterferon alfa plus ribavirin...
- Peginterferon alfa-2b plus ribavirin compared with interferon alfa-2b plus ribavirin for initial treatment of chronic hepatitis C: a randomised trial
M P Manns
Division of Gastroenterology and Hepatology, Medical School of Hannover, Hannover, Germany
Lancet 358:958-65. 2001
..In this randomised trial, peginterferon alfa-2b plus ribavirin was compared with interferon alfa-2b plus ribavirin...
- Response-guided telaprevir combination treatment for hepatitis C virus infection
Kenneth E Sherman
Division of Digestive Diseases, University of Cincinnati College of Medicine, Cincinnati, OH 45267 0595, USA
N Engl J Med 365:1014-24. 2011
..We designed a noninferiority trial (noninferiority margin, -10.5%) to compare rates of sustained virologic response among patients receiving two treatment durations...
- Surviving Sepsis Campaign: international guidelines for management of severe sepsis and septic shock: 2008
R Phillip Dellinger
Cooper University Hospital, Camden, NJ, USA
Crit Care Med 36:296-327. 2008
..To provide an update to the original Surviving Sepsis Campaign clinical management guidelines, "Surviving Sepsis Campaign Guidelines for Management of Severe Sepsis and Septic Shock," published in 2004...
- Generation of induced pluripotent stem cells using recombinant proteins
Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA
Cell Stem Cell 4:381-4. 2009
- Binding of hepatitis C virus to CD81
IRIS, Chiron, Siena 53100, Italy
Science 282:938-41. 1998
..Binding of E2 was mapped to the major extracellular loop of CD81. Recombinant molecules containing this loop bound HCV and antibodies that neutralize HCV infection in vivo inhibited virus binding to CD81 in vitro...
- Telaprevir and peginterferon with or without ribavirin for chronic HCV infection
Assistance Publique Hopitaux de Paris, Henri Mondor Hospital, University of Paris 12 and INSERM Unité 955, Creteil, France
N Engl J Med 360:1839-50. 2009
..Telaprevir is a specific inhibitor of the HCV serine protease and could be of value in HCV treatment...
- Identification of a major co-receptor for primary isolates of HIV-1
Skirball Institute for Biomolecular Medicine, New York University Medical Center, 10016, USA
Nature 381:661-6. 1996
..The principal cofactor for entry mediated by the envelope glycoproteins of primary macrophage-tropic strains of HIV-1 is CC-CKR-5, a receptor for the beta-chemokines RANTES, MIP-1alpha and MIP-1beta...
- High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells
Animal Cell Technology and Downstream Processing Group, Biotechnology Research Institute, National Research Council Canada, 6100 Royalmount Avenue, Montreal, Quebec H4P 2R2, Canada
Nucleic Acids Res 30:E9. 2002
..Using the pTT vector, >20 mg/l of purified His-tagged SEAP was recovered from a 3.5 l bioreactor. Intracellular proteins were also produced at levels as high as 50 mg/l, representing up to 20% of total cell proteins...
- Genetic variation in IL28B is associated with chronic hepatitis C and treatment failure: a genome-wide association study
University Clinic of Infectious Diseases, University Hospital Bern and University of Bern, Bern, Switzerland
Gastroenterology 138:1338-45, 1345.e1-7. 2010
..We performed a genome-wide association study to screen for host genetic determinants of HCV persistence and response to therapy...
- Green fluorescent protein as a marker for gene expression
Department of Biological Sciences, Columbia University, New York, NY 10027
Science 263:802-5. 1994
..Because exogenous substrates and cofactors are not required for this fluorescence, GFP expression can be used to monitor gene expression and protein localization in living organisms...
- Normalization of hemoglobin level in patients with chronic kidney disease and anemia
Tilman B Drueke
Inserm Unité 507 and Assistance Publique Hôpitaux de Paris, Necker Hospital, Division of Nephrology, Paris, France
N Engl J Med 355:2071-84. 2006
..Whether correction of anemia in patients with stage 3 or 4 chronic kidney disease improves cardiovascular outcomes is not established...
- Preliminary study of two antiviral agents for hepatitis C genotype 1
Anna S Lok
Department of Internal Medicine, Division of Gastroenterology, 3912 Taubman Center, SPC 5362, University of Michigan Medical Center, Ann Arbor, MI 48109, USA
N Engl J Med 366:216-24. 2012
..Patients with chronic hepatitis C virus (HCV) infection who have not had a response to therapy with peginterferon and ribavirin may benefit from the addition of multiple direct-acting antiviral agents to their treatment regimen...
- Correction of anemia with epoetin alfa in chronic kidney disease
Ajay K Singh
Renal Division, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
N Engl J Med 355:2085-98. 2006
..Recombinant human erythropoietin (epoetin alfa) is indicated for the correction of anemia associated with this condition. However, the optimal level of hemoglobin correction is not defined...
- Functional interaction of beta-catenin with the transcription factor LEF-1
Max Delbruck Centre for Molecular Medicine, Berlin, Germany
Nature 382:638-42. 1996
..Thus beta-catenin regulates gene expression by direct interaction with transcription factors such as LEF-1, providing a molecular mechanism for the transmission of signals, from cell-adhesion components or wnt protein to the nucleus...
- HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases NIAID, NIH, Bethesda, MD 20892, USA
Science 272:872-7. 1996
..Fusin messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types. Fusin acted preferentially for T cell line-tropic isolates, in comparison to its activity with macrophagetropic HIV-1 isolates...
- The human scavenger receptor class B type I is a novel candidate receptor for the hepatitis C virus
Istituto di Ricerche di Biologia Molecolare P Angeletti IRBM, Via Pontina Km 30 600, 00040 Pomezia Rome, Italy
EMBO J 21:5017-25. 2002
..Finally, E2 recognition by SR-BI was competed out in an isolate-specific manner both on the hepatoma cell line and on the human SR-BI-transfected cell line by an anti-HVR1 monoclonal antibody...
- Effects of the obese gene product on body weight regulation in ob/ob mice
M A Pelleymounter
Department of Neurobiology, Amgen, Inc, Thousand Oaks, CA 91320, USA
Science 269:540-3. 1995
..These data suggest that the OB protein regulates body weight and fat deposition through effects on metabolism and appetite...
- Genome sequence of the recombinant protein production host Pichia pastoris
Kristof De Schutter
Unit for Molecular Glycobiology, Department for Molecular Biomedical Research, VIB, Ghent Zwijnaarde, Belgium
Nat Biotechnol 27:561-6. 2009
..pastoris. To facilitate progress in these areas, we present the 9.43 Mbp genomic sequence of the GS115 strain of P. pastoris. We also provide manually curated annotation for its 5,313 protein-coding genes...
- A time- and cost-efficient system for high-level protein production in mammalian cells
A Radu Aricescu
Cancer Research UK Receptor Structure Research Group, Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, England
Acta Crystallogr D Biol Crystallogr 62:1243-50. 2006
..The system is suitable for use in conventional laboratories or can be implemented in a medium- or high-throughput pipeline...
- An improved cyan fluorescent protein variant useful for FRET
Mark A Rizzo
Nat Biotechnol 22:445-9. 2004
..Cerulean is 2.5-fold brighter than ECFP and replacement of ECFP with Cerulean substantially improves the signal-to-noise ratio of a FRET-based sensor for glucokinase activation...
- Peginterferon alfa-2b or alfa-2a with ribavirin for treatment of hepatitis C infection
John G McHutchison
Duke Clinical Research Institute, Duke University Medical Center, Durham, NC 27715, USA
N Engl J Med 361:580-93. 2009
..Treatment guidelines recommend the use of peginterferon alfa-2b or peginterferon alfa-2a in combination with ribavirin for chronic hepatitis C virus (HCV) infection. However, these regimens have not been adequately compared...
- High mobility group 1 protein (HMG-1) stimulates proinflammatory cytokine synthesis in human monocytes
Department of Medicine, Rheumatology Unit, Karolinska Hospital, 17176 Stockholm, Sweden
J Exp Med 192:565-70. 2000
..Together, these results indicate that, like other cytokine mediators of endotoxin lethality (e.g., TNF and IL-1), extracellular HMG-1 is a regulator of monocyte proinflammatory cytokine synthesis...
- Heterologous protein production using the Pichia pastoris expression system
Strathclyde Fermentation Centre, Department of Bioscience, University of Strathclyde, 204 George Street, Glasgow G1 1XW, UK
Yeast 22:249-70. 2005
..The problems associated with post-translational modifications performed on recombinant proteins by P...
- 2010 update of EORTC guidelines for the use of granulocyte-colony stimulating factor to reduce the incidence of chemotherapy-induced febrile neutropenia in adult patients with lymphoproliferative disorders and solid tumours
M S Aapro
Multidisciplinary Oncology Institute, Clinique de Genolier, 1, route du Muids, 1272 Genolier, Switzerland
Eur J Cancer 47:8-32. 2011
..Evidence from multiple low level studies derived from audit data and clinical practice suggests that some patients receive suboptimal daily G-CSFs; the use of pegfilgrastim may avoid this problem...
- Telaprevir for previously treated chronic HCV infection
John G McHutchison
Duke Clinical Research Institute and Duke University Medical Center, Durham, NC, USA
N Engl J Med 362:1292-303. 2010
..Patients with genotype 1 hepatitis C virus (HCV) who do not have a sustained response to therapy with peginterferon alfa and ribavirin have a low likelihood of success with retreatment...
- Recombinant protein expression in Escherichia coli
Department of Chemical Engineering University of Washington Box 351750, Seattle, WA 98195, USA
Curr Opin Biotechnol 10:411-21. 1999
..coli, together with serendipitous discoveries and the availability of improved genetic tools are making this bacterium more valuable than ever for the expression of complex eukaryotic proteins...
- Identification and characterization of a family of mammalian methyl-CpG binding proteins
Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR, Scotland
Mol Cell Biol 18:6538-47. 1998
..The data demonstrate that MBD2 and MBD4 bind specifically to methyl-CpG in vitro and in vivo and are therefore likely to be mediators of the biological consequences of the methylation signal...
- Fibroblast growth factor 21 reverses hepatic steatosis, increases energy expenditure, and improves insulin sensitivity in diet-induced obese mice
Department of Metabolic Disorders, Amgen, Thousand Oaks, California, USA
Diabetes 58:250-9. 2009
..The aims of the current study are to evaluate the role of FGF21 in energy metabolism and to provide mechanistic insights into its glucose and lipid-lowering effects in a high-fat diet-induced obesity (DIO) model...
- Telaprevir with peginterferon and ribavirin for treatment-naive patients chronically infected with HCV of genotype 1 in Japan
Department of Hepatology, Toranomon Hospital, Tokyo, Japan
J Hepatol 56:78-84. 2012
..To evaluate the efficacy and safety of telaprevir in combination with peginterferon-α2b (PEG-IFN) and ribavirin (RBV) in patients with chronic hepatitis C...
- Hepatic ISG expression is associated with genetic variation in interleukin 28B and the outcome of IFN therapy for chronic hepatitis C
Department of Gastroenterology, Kanazawa University Graduate School of Medicine, Kanazawa, Japan
Gastroenterology 139:499-509. 2010
..Multiple viral and host factors are related to the treatment response to pegylated-interferon and ribavirin combination therapy; however, the clinical relevance and relationship of these factors have not yet been fully evaluated...
- The blood-stage malaria antigen PfRH5 is susceptible to vaccine-inducible cross-strain neutralizing antibody
Alexander D Douglas
Jenner Institute, University of Oxford, Old Road Campus Research Building, Roosevelt Drive, Oxford OX3 7DQ, UK
Nat Commun 2:601. 2011
..falciparum is a degenerate process involving a series of parallel redundant pathways...
- Structure-function studies of the adipocyte-secreted hormone Acrp30/adiponectin. Implications fpr metabolic regulation and bioactivity
Utpal B Pajvani
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
J Biol Chem 278:9073-85. 2003
..Combined, these results shed new light on the regulation of this complex protein and suggest a new model for in vivo activation of the protein, implicating a serum reductase activity...
- Nuclear export of microRNA precursors
Department of Biomolecular Chemistry, University of Wisconsin Medical School, Madison, WI 53706, USA
Science 303:95-8. 2004
..Thus, Exp5 is key to miRNA biogenesis and may help coordinate nuclear and cytoplasmic processing steps...
- Crystal structure of a mammalian voltage-dependent Shaker family K+ channel
Stephen B Long
Howard Hughes Medical Institute, Laboratory of Molecular Neurobiology and Biophysics, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
Science 309:897-903. 2005
..Electrostatic properties of the side portals and positions of the T1 domain and beta subunit are consistent with electrophysiological studies of inactivation gating and with the possibility of K+ channel regulation by the beta subunit...
- Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3
Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, 75235, USA
Cell 90:405-13. 1997
..The COOH-terminal region of Apaf-1 comprises multiple WD repeats, which are proposed to mediate protein-protein interactions. Cytochrome c binds to Apaf-1, an event that may trigger the activation of caspase-3, leading to apoptosis...
- Interferon signaling and treatment outcome in chronic hepatitis C
Department of Biomedicine, University of Basel, CH 4031 Basel, Switzerland
Proc Natl Acad Sci U S A 105:7034-9. 2008
- Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production
ARC Special Research Centre for Green Chemistry, Monash University, Building 23, Wellington Road, Clayton, Victoria 3800, Australia
J Mol Recognit 18:119-38. 2005
The use of the methylotrophic yeast, Pichia pastoris, as a cellular host for the expression of recombinant proteins has become increasing popular in recent times. P...
- Advanced genetic strategies for recombinant protein expression in Escherichia coli
Hans Peter Sørensen
Laboratory of BioDesign, Department of Molecular Biology, Aarhus University, Gustav Wieds Vej 10 C, DK 8000 Aarhus C, Denmark
J Biotechnol 115:113-28. 2005
..The macromolecular background for a variety of obstacles and genetic state-of-the-art solutions are presented...
- Cell-free translation reconstituted with purified components
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Bldg FSB401, Kasiwanoha, Kashiwa, Chiba Prefecture 277-8562, Japan
Nat Biotechnol 19:751-5. 2001
..Moreover, omission of a release factor allowed efficient incorporation of an unnatural amino acid using suppressor transfer RNA (tRNA)...
- Dynamic hepatitis C virus genotypic and phenotypic changes in patients treated with the protease inhibitor telaprevir
Klinik fur Innere Medizin II, Universitat des Saarlandes, Homburg Saar, Germany
Gastroenterology 132:1767-77. 2007
..4A protease inhibitor, has shown strong antiviral activity in phase 1 clinical studies. Because of high levels of HCV replication and the low fidelity of HCV polymerase, selection of resistant isolates during therapy may occur...
- Replicated association between an IL28B gene variant and a sustained response to pegylated interferon and ribavirin
Jeanette J McCarthy
Institute for Genome Sciences and Policy, Duke University Medical Center, Durham, North Carolina 27708, USA
Gastroenterology 138:2307-14. 2010
..We investigated the association between the polymorphism rs12979860 and treatment response in a diverse cohort of chronic HCV patients...
- Phase I dose-escalation study of recombinant human Apo2L/TRAIL, a dual proapoptotic receptor agonist, in patients with advanced cancer
Roy S Herbst
University of Texas M D Anderson Cancer Center, Thoracic Head and Neck Medicine Clinic, 1515 Holcombe Blvd, Unit 432, Houston, TX 77030, USA
J Clin Oncol 28:2839-46. 2010
..To the best of our knowledge, this is the first in-human clinical trial to assess the safety, tolerability, pharmacokinetics, and antitumor activity of multiple intravenous doses of rhApo2L/TRAIL in patients with advanced cancer...
- Overproduction of recombinant proteins in plants
Applied Biotechnology Institute, Cal Poly Technology Park, Building 83, San Luis Obispo, CA 93407, USA
Plant Sci 184:83-101. 2012
..Plants as alternative hosts for the production of recombinant proteins are being actively pursued, taking advantage of their unique characteristics...
- TRAIL receptor signalling and modulation: Are we on the right TRAIL?
Department of Biochemistry and National Centre for Biomedical Engineering Science, National University of Ireland, Galway, University Road, Galway, Ireland
Cancer Treat Rev 35:280-8. 2009
- Genome-scale metabolic reconstruction and in silico analysis of methylotrophic yeast Pichia pastoris for strain improvement
Bevan Ks Chung
NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, 28 Medical Drive, 05 01, 117456, Singapore
Microb Cell Fact 9:50. 2010
..pastoris can be employed together with high throughput experimental data analysis, for better understanding of the methylotrophic yeast's metabolism...
- High-throughput production of human proteins for crystallization: the SGC experience
Structural Genomics Consortium, University of Oxford, Old Road Campus Research Building, Oxford, UK
J Struct Biol 172:3-13. 2010
..These can be used to formulate guidelines for initial approaches to expression of new eukaryotic proteins...
- Interferon-induced gene expression is a stronger predictor of treatment response than IL28B genotype in patients with hepatitis C
Michael T Dill
Department of Biomedicine, Hepatology Laboratory, University of Basel, Basel, Switzerland
Gastroenterology 140:1021-31. 2011
..We investigated whether IL28B genotype determines the constitutive expression of ISGs in the liver and compared the abilities of ISG levels and IL28B genotype to predict treatment outcome...
- T cell interleukin-17 induces stromal cells to produce proinflammatory and hematopoietic cytokines
Schering Plough, Laboratory for Immunological Research, Dardilly, France
J Exp Med 183:2593-603. 1996
..These observations suggest that hIL-17 may constitute (a) an early initiator of the T cell-dependent inflammmatory reaction; and (b) an element of the cytokine network that bridges the immune system to hematopoiesis...
- Role of interleukin-28B polymorphisms in the treatment of hepatitis C virus genotype 2 infection in Asian patients
Ming Lung Yu
Department of Internal Medicine, Kaohsiung Municipal Ta Tung Hospital, Kaohsiung, Taiwan
Hepatology 53:7-13. 2011
..99-1.00, P = 0.04). Nevertheless, the achievement of RVR was the single predictor of SVR with an OR of 19.37 (95% CI = 8.89-42.23, P < 0.001), whereas the rs8099917 genotypes played no role in achieving SVR with or without RVR...
- IP-10 predicts viral response and therapeutic outcome in difficult-to-treat patients with HCV genotype 1 infection
Department of Infectious Diseases, University of Goteborg, Sweden
Hepatology 44:1617-25. 2006
..Thus, pretreatment IP-10 analysis may prove helpful in decision-making regarding pharmaceutical intervention...
- Insulin resistance impairs sustained response rate to peginterferon plus ribavirin in chronic hepatitis C patients
Hepatology Unit, Hospital Universitario de Valme, Sevilla, Spain
Gastroenterology 128:636-41. 2005
..We evaluated the effect of insulin resistance and viral factors on sustained virological response in patients with chronic hepatitis C treated with peginterferon plus ribavirin...
- Randomized study of peginterferon-alpha2a plus ribavirin vs peginterferon-alpha2b plus ribavirin in chronic hepatitis C
Maria Grazia Rumi
AM Migliavacca Center for Liver Disease, First Division of Gastroenterology, Fondazione IRCCS Maggiore Hospital, Mangiagalli e Regina Elena, Universita degli Studi di Milano, Milan, Italy
Gastroenterology 138:108-15. 2010
..Due to the lack of head-to-head studies, the 2 PegIFNs have not been directly compared. The endpoints of our study were safety and antiviral efficacy of the 2 regimens...
- Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 A resolution
R B Sutton
The Howard Hughes Medical Institute, Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
Nature 395:347-53. 1998
..These characteristics may be important for membrane fusion and for the binding of regulatory factors affecting neurotransmission...
- Crystal structure of the activated insulin receptor tyrosine kinase in complex with peptide substrate and ATP analog
S R Hubbard
Department of Pharmacology and Skirball Institute of Biomolecular Medicine, 540 First Avenue, New York University Medical Center, New York, NY 10016, USA
EMBO J 16:5572-81. 1997
..The structure thus reveals the molecular basis for insulin receptor activation via autophosphorylation, and provides insights into tyrosine kinase substrate specificity and the mechanism of phosphotransfer...
- The Anopheles gambiae odorant binding protein 1 (AgamOBP1) mediates indole recognition in the antennae of female mosquitoes
Developmental Biology Center, Department of Molecular Biology and Biochemistry, University of California Irvine, Irvine, California, United States of America
PLoS ONE 5:e9471. 2010
..gambiae OBP-ligand pairing combination, demonstrates the significance of OBPs in odor recognition, and can be expanded to the identification of other ligands for OBPs of Anopheles and other medically important insects...
- Assignment of intrachain disulfide bonds and characterization of potential glycosylation sites of the type 1 recombinant human immunodeficiency virus envelope glycoprotein (gp120) expressed in Chinese hamster ovary cells
C K Leonard
Department of Medicinal Chemistry, Genentech, Inc, South San Francisco, California 94080
J Biol Chem 265:10373-82. 1990
- CHO cells in biotechnology for production of recombinant proteins: current state and further potential
Jee Yon Kim
Department of Biological Sciences, Graduate School of Nanoscience and Technology WCU, KAIST, 373 1 Kusong Dong, Yuseong Gu, Daejeon 305 701, Korea
Appl Microbiol Biotechnol 93:917-30. 2012
- Peginterferon Alfa-2a, lamivudine, and the combination for HBeAg-positive chronic hepatitis B
George K K Lau
Department of Medicine, Queen Mary Hospital, University of Hong Kong, Hong Kong SAR, China
N Engl J Med 352:2682-95. 2005
- Bax directly induces release of cytochrome c from isolated mitochondria
J M Jurgensmeier
Program on Apoptosis and Cell Death Research, The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA
Proc Natl Acad Sci U S A 95:4997-5002. 1998
- Ligand-based targeting of apoptosis in cancer: the potential of recombinant human apoptosis ligand 2/Tumor necrosis factor-related apoptosis-inducing ligand (rhApo2L/TRAIL)
Department of Molecular Oncology, Genentech Inc, South San Francisco, CA 94080, USA
J Clin Oncol 26:3621-30. 2008
..Clinical studies are ongoing to assess the safety and efficacy of this novel agent in combination with established anticancer therapies...
- Recombinant protein production in a variety of Nicotiana hosts: a comparative analysis
Andrew J Conley
Department of Biology, University of Western Ontario, London, ON, Canada
Plant Biotechnol J 9:434-44. 2011
..biopharmaceuticals and industrial enzymes, tobacco has the most established history for the production of recombinant proteins. To further improve the heterologous protein yield of tobacco platforms, transient and stable expression of ..
- Pre-expression of a sulfhydryl oxidase significantly increases the yields of eukaryotic disulfide bond containing proteins expressed in the cytoplasm of E.coli
Van Dat Nguyen
Department of Biochemistry, Linnanmaa campus, University of Oulu, 90570 Oulu, Finland
Microb Cell Fact 10:1. 2011
..We have recently shown that it is possible to produce large amounts of prokaryotic disulfide bond containing proteins in the cytoplasm of wild-type bacteria such as E. coli by the introduction of catalysts for both of these processes...
- Telaprevir with peginterferon and ribavirin for chronic HCV genotype 1 infection
John G McHutchison
Duke Clinical Research Institute and Duke University, Durham, NC 27715, USA
N Engl J Med 360:1827-38. 2009
..Telaprevir, a protease inhibitor specific to the HCV nonstructural 3/4A serine protease, rapidly reduced HCV RNA levels in early studies...
- Efficacy of boceprevir, an NS3 protease inhibitor, in combination with peginterferon alfa-2b and ribavirin in treatment-naive patients with genotype 1 hepatitis C infection (SPRINT-1): an open-label, randomised, multicentre phase 2 trial
Paul Y Kwo
Department of Medicine, Division of Gastroenterology Hepatology, Indiana University School of Medicine, Indianapolis, IN 46202 5121, USA
Lancet 376:705-16. 2010
..We tested the efficacy of boceprevir, an NS3 hepatitis C virus oral protease inhibitor, when added to peginterferon alfa-2b and ribavirin...
- Association between naturally acquired antibodies to erythrocyte-binding antigens of Plasmodium falciparum and protection from malaria and high-density parasitemia
Jack S Richards
Department of Medical Biology, Walter and Eliza Hall Institute of Medical Research, University of Melbourne, Victoria, Australia
Clin Infect Dis 51:e50-60. 2010
..Erythrocyte-binding antigens (EBAs) are important erythrocyte invasion ligands used by merozoites and may be targets of protective immunity, but there are limited data examining their potential importance...
- Histone H4-K16 acetylation controls chromatin structure and protein interactions
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
Science 311:844-7. 2006
- The metabolic state of diabetic monkeys is regulated by fibroblast growth factor-21
Lilly Research Laboratories, A Division of Eli Lilly and Company, Indianapolis, Indiana 46285, USA
Endocrinology 148:774-81. 2007
..These data support the development of FGF-21 for the treatment of diabetes and other metabolic diseases...
- Engineering of protein secretion in yeast: strategies and impact on protein production
R and D Group, ASPEX Division, Research Center, Asahi Glass Co, Ltd, 1150 Hazawa cho, Kanagawa ku, Yokohama, 221 8755, Japan
Appl Microbiol Biotechnol 86:403-17. 2010
..This mini-review focuses on recent strategies and their advantages for systematic engineering of yeast strains for effective protein secretion...
- HEK293 cell line: a vehicle for the expression of recombinant proteins
Department of Pharmacology, University College London, Gower Street, London, WC1E 6BT, UK
J Pharmacol Toxicol Methods 51:187-200. 2005
The HEK cell line has been extensively used as an expression tool for recombinant proteins since it was generated over 25 years ago...
- A randomized double-blind multicenter phase III study of fixed-dose single-administration pegfilgrastim versus daily filgrastim in patients receiving myelosuppressive chemotherapy
M D Green
Royal Melbourne Hospital, Melbourne, Australia
Ann Oncol 14:29-35. 2003
..We evaluated the efficacy of a single fixed 6 mg dose of pegfilgrastim (a pegylated version of filgrastim) per cycle of chemotherapy, compared with daily administration of filgrastim, in the provision of neutrophil support...
- Peginterferon alfa-2a plus ribavirin is more effective than peginterferon alfa-2b plus ribavirin for treating chronic hepatitis C virus infection
Department of Gastroenterology, Liver Unit, Cardarelli Hospital, Napoli, Italy
Gastroenterology 138:116-22. 2010
..This study compared the efficacy and safety of peginterferon alfa-2a and peginterferon alfa-2b, each in combination with ribavirin...
- Functional amyloid formation within mammalian tissue
Douglas M Fowler
Department of Chemistry, The Skaggs Institute of Chemical Biology, The Scripps Research Institute, La Jolla, California, USA
PLoS Biol 4:e6. 2006
- A companion protease inhibitor for the protection of cytosol-targeted recombinant proteins in plants
CRH INAF, Pavillon des Services INAF, Universite Laval, Quebec, QC, Canada
Plant Biotechnol J 8:142-54. 2010
..potential of tomato cathepsin D inhibitor (SlCDI) as an in-built stabilizing agent for the protection of recombinant proteins in transgenic plant leaf crude extracts (Plant Biotechnol J.4, 359-368)...
- Vaccination against Neisseria meningitidis using three variants of the lipoprotein GNA1870
IRIS, Chiron Srl, Via Fiorentina 1, 53100 Siena, Italy
J Exp Med 197:789-99. 2003
..The novel antigen is a top candidate for the development of a new vaccine against meningococcus...
- Mucosal delivery of therapeutic and prophylactic molecules using lactic acid bacteria
Jerry M Wells
Host Microbe Interactomics, University of Wageningen, Animal Sciences Department, P O Box 338, 6700 AH, Wageningen, The Netherlands
Nat Rev Microbiol 6:349-62. 2008
- Erythropoietin-mediated tissue protection: reducing collateral damage from the primary injury response
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NIZO Food Research, P O Box 20, 6710, Ede, The Netherlands
Appl Microbiol Biotechnol 68:705-17. 2005
..Finally, an overview is given of growth and induction conditions for lab-scale and industrial-scale applications...
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Nature 378:88-91. 1995
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Departments of Cell Biology and Chemistry, The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California 92037, USA
Annu Rev Biochem 74:411-32. 2005
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Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
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Wyeth Vaccines Research, Pearl River, New York 10965, USA
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Julian K C Ma
Department of Infectious Diseases, St George s Hospital Medical School, Cranmer Terrace, London SW17 0RE, UK
Nat Rev Genet 4:794-805. 2003
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Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA
J Virol 77:642-58. 2003
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Howard Hughes Medical Institute, University of California San Francisco, San Francisco, California, United States of America
PLoS Biol 4:e423. 2006
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Michael W Fried
Division of Digestive Diseases, University of North Carolina, Chapel Hill, North Carolina 27599, USA
Hepatology 36:S237-44. 2002
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Instituto de Productos Lacteos de Asturias IPLA CSIC, Apdo, Asturias, Spain
BMC Microbiol 11:138. 2011
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Nezam H Afdhal
Division of Gastroenterology and Hepatology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02215, USA
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Blood 118:5767-73. 2011
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Department of Medicine and Clinical Oncology, Graduate School of Medicine, Chiba University, Chiba, Japan
PLoS ONE 6:e28617. 2011
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Institut Pasteur, Unité de Biologie des Interactions Hôte Parasite, Centre National de la Recherche Scientifique Unité de Recherche Associée 2581, 25 rue du Docteur Roux, F 75724 Paris Cedex 15, France
Proc Natl Acad Sci U S A 107:4884-9. 2010
..These results have important consequences for the development of an effective vaccine and therapeutic inhibitors...
- Cloning of a disintegrin metalloproteinase that processes precursor tumour-necrosis factor-alpha
M L Moss
Department of Molecular Biochemistry, Glaxo Wellcome Research and Development Inc, Research Triangle Park, North Carolina 27709, USA
Nature 385:733-6. 1997
..The rTACE provides a readily available source of enzyme to help in the search for new anti-inflammatory agents that target the final processing stage of TNF-alpha production...