- Genetic variation in IL28B predicts hepatitis C treatment-induced viral clearance
Institute for Genome Sciences and Policy, Center for Human Genome Variation, Duke University, Durham, North Carolina 27708, USA
Nature 461:399-401. 2009
- Peginterferon alfa-2a plus ribavirin for chronic hepatitis C virus infection
Michael W Fried
University of North Carolina, Chapel Hill 27599, USA
N Engl J Med 347:975-82. 2002
..We compared the efficacy and safety of peginterferon alfa-2a plus ribavirin, interferon alfa-2b plus ribavirin, and peginterferon alfa-2a alone in the initial treatment of chronic hepatitis C...
- Protein production by auto-induction in high density shaking cultures
F William Studier
Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA
Protein Expr Purif 41:207-34. 2005
..Selenomethionine labeling was equally efficient in the commonly used methionine auxotroph B834(DE3) (found to be metE) or the prototroph BL21(DE3)...
- Peginterferon alfa-2b plus ribavirin compared with interferon alfa-2b plus ribavirin for initial treatment of chronic hepatitis C: a randomised trial
M P Manns
Division of Gastroenterology and Hepatology, Medical School of Hannover, Hannover, Germany
Lancet 358:958-65. 2001
..In this randomised trial, peginterferon alfa-2b plus ribavirin was compared with interferon alfa-2b plus ribavirin...
- Peginterferon-alpha2a and ribavirin combination therapy in chronic hepatitis C: a randomized study of treatment duration and ribavirin dose
Stephanos J Hadziyannis
Department of Medicine and Hepatology, Henry Dunant Hospital, Athens, Greece
Ann Intern Med 140:346-55. 2004
..Treatment with pegylated interferon (peginterferon) and ribavirin for 48 weeks is more effective than conventional interferon and ribavirin in patients with chronic hepatitis C...
- Telaprevir with peginterferon and ribavirin for chronic HCV genotype 1 infection
John G McHutchison
Duke Clinical Research Institute and Duke University, Durham, NC 27715, USA
N Engl J Med 360:1827-38. 2009
..Telaprevir, a protease inhibitor specific to the HCV nonstructural 3/4A serine protease, rapidly reduced HCV RNA levels in early studies...
- Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein
Nathan C Shaner
Nat Biotechnol 22:1567-72. 2004
..Three monomers with distinguishable hues from yellow-orange to red-orange have higher quantum efficiencies...
- Telaprevir and peginterferon with or without ribavirin for chronic HCV infection
Assistance Publique Hopitaux de Paris, Henri Mondor Hospital, University of Paris 12 and INSERM Unité 955, Creteil, France
N Engl J Med 360:1839-50. 2009
..Telaprevir is a specific inhibitor of the HCV serine protease and could be of value in HCV treatment...
- Telaprevir for previously treated chronic HCV infection
John G McHutchison
Duke Clinical Research Institute and Duke University Medical Center, Durham, NC, USA
N Engl J Med 362:1292-303. 2010
..Patients with genotype 1 hepatitis C virus (HCV) who do not have a sustained response to therapy with peginterferon alfa and ribavirin have a low likelihood of success with retreatment...
- Peginterferon alfa-2b or alfa-2a with ribavirin for treatment of hepatitis C infection
John G McHutchison
Duke Clinical Research Institute, Duke University Medical Center, Durham, NC 27715, USA
N Engl J Med 361:580-93. 2009
..Treatment guidelines recommend the use of peginterferon alfa-2b or peginterferon alfa-2a in combination with ribavirin for chronic hepatitis C virus (HCV) infection. However, these regimens have not been adequately compared...
- Genetic variation in IL28B is associated with chronic hepatitis C and treatment failure: a genome-wide association study
University Clinic of Infectious Diseases, University Hospital Bern and University of Bern, Bern, Switzerland
Gastroenterology 138:1338-45, 1345.e1-7. 2010
..We performed a genome-wide association study to screen for host genetic determinants of HCV persistence and response to therapy...
- High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells
Animal Cell Technology and Downstream Processing Group, Biotechnology Research Institute, National Research Council Canada, 6100 Royalmount Avenue, Montreal, Quebec H4P 2R2, Canada
Nucleic Acids Res 30:E9. 2002
..Using the pTT vector, >20 mg/l of purified His-tagged SEAP was recovered from a 3.5 l bioreactor. Intracellular proteins were also produced at levels as high as 50 mg/l, representing up to 20% of total cell proteins...
- Functional interaction of beta-catenin with the transcription factor LEF-1
Max Delbruck Centre for Molecular Medicine, Berlin, Germany
Nature 382:638-42. 1996
..Thus beta-catenin regulates gene expression by direct interaction with transcription factors such as LEF-1, providing a molecular mechanism for the transmission of signals, from cell-adhesion components or wnt protein to the nucleus...
- HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases NIAID, NIH, Bethesda, MD 20892, USA
Science 272:872-7. 1996
..Fusin messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types. Fusin acted preferentially for T cell line-tropic isolates, in comparison to its activity with macrophagetropic HIV-1 isolates...
- Generation of induced pluripotent stem cells using recombinant proteins
Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA
Cell Stem Cell 4:381-4. 2009
- Effects of the obese gene product on body weight regulation in ob/ob mice
M A Pelleymounter
Department of Neurobiology, Amgen, Inc, Thousand Oaks, CA 91320, USA
Science 269:540-3. 1995
..These data suggest that the OB protein regulates body weight and fat deposition through effects on metabolism and appetite...
- Correction of anemia with epoetin alfa in chronic kidney disease
Ajay K Singh
Renal Division, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
N Engl J Med 355:2085-98. 2006
..Recombinant human erythropoietin (epoetin alfa) is indicated for the correction of anemia associated with this condition. However, the optimal level of hemoglobin correction is not defined...
- Recombinant protein expression in Escherichia coli
Department of Chemical Engineering University of Washington Box 351750, Seattle, WA 98195, USA
Curr Opin Biotechnol 10:411-21. 1999
..coli, together with serendipitous discoveries and the availability of improved genetic tools are making this bacterium more valuable than ever for the expression of complex eukaryotic proteins...
- Nuclear export of microRNA precursors
Department of Biomolecular Chemistry, University of Wisconsin Medical School, Madison, WI 53706, USA
Science 303:95-8. 2004
..Thus, Exp5 is key to miRNA biogenesis and may help coordinate nuclear and cytoplasmic processing steps...
- Surviving Sepsis Campaign: international guidelines for management of severe sepsis and septic shock: 2008
R Phillip Dellinger
Cooper University Hospital, Camden, NJ, USA
Crit Care Med 36:296-327. 2008
..To provide an update to the original Surviving Sepsis Campaign clinical management guidelines, "Surviving Sepsis Campaign Guidelines for Management of Severe Sepsis and Septic Shock," published in 2004...
- Telaprevir with peginterferon and ribavirin for treatment-naive patients chronically infected with HCV of genotype 1 in Japan
Department of Hepatology, Toranomon Hospital, Tokyo, Japan
J Hepatol 56:78-84. 2012
..To evaluate the efficacy and safety of telaprevir in combination with peginterferon-α2b (PEG-IFN) and ribavirin (RBV) in patients with chronic hepatitis C...
- Binding of hepatitis C virus to CD81
IRIS, Chiron, Siena 53100, Italy
Science 282:938-41. 1998
..Binding of E2 was mapped to the major extracellular loop of CD81. Recombinant molecules containing this loop bound HCV and antibodies that neutralize HCV infection in vivo inhibited virus binding to CD81 in vitro...
- Interferon signaling and treatment outcome in chronic hepatitis C
Department of Biomedicine, University of Basel, CH 4031 Basel, Switzerland
Proc Natl Acad Sci U S A 105:7034-9. 2008
- Identification of a major co-receptor for primary isolates of HIV-1
Skirball Institute for Biomolecular Medicine, New York University Medical Center, 10016, USA
Nature 381:661-6. 1996
..The principal cofactor for entry mediated by the envelope glycoproteins of primary macrophage-tropic strains of HIV-1 is CC-CKR-5, a receptor for the beta-chemokines RANTES, MIP-1alpha and MIP-1beta...
- Dynamic hepatitis C virus genotypic and phenotypic changes in patients treated with the protease inhibitor telaprevir
Klinik fur Innere Medizin II, Universitat des Saarlandes, Homburg Saar, Germany
Gastroenterology 132:1767-77. 2007
..4A protease inhibitor, has shown strong antiviral activity in phase 1 clinical studies. Because of high levels of HCV replication and the low fidelity of HCV polymerase, selection of resistant isolates during therapy may occur...
- Replicated association between an IL28B gene variant and a sustained response to pegylated interferon and ribavirin
Jeanette J McCarthy
Institute for Genome Sciences and Policy, Duke University Medical Center, Durham, North Carolina 27708, USA
Gastroenterology 138:2307-14. 2010
..We investigated the association between the polymorphism rs12979860 and treatment response in a diverse cohort of chronic HCV patients...
- Normalization of hemoglobin level in patients with chronic kidney disease and anemia
Tilman B Drueke
Inserm Unité 507 and Assistance Publique Hôpitaux de Paris, Necker Hospital, Division of Nephrology, Paris, France
N Engl J Med 355:2071-84. 2006
..Whether correction of anemia in patients with stage 3 or 4 chronic kidney disease improves cardiovascular outcomes is not established...
- Genome sequence of the recombinant protein production host Pichia pastoris
Kristof De Schutter
Unit for Molecular Glycobiology, Department for Molecular Biomedical Research, VIB, Ghent Zwijnaarde, Belgium
Nat Biotechnol 27:561-6. 2009
..pastoris. To facilitate progress in these areas, we present the 9.43 Mbp genomic sequence of the GS115 strain of P. pastoris. We also provide manually curated annotation for its 5,313 protein-coding genes...
- Role of interleukin-28B polymorphisms in the treatment of hepatitis C virus genotype 2 infection in Asian patients
Ming Lung Yu
Department of Internal Medicine, Kaohsiung Municipal Ta Tung Hospital, Kaohsiung, Taiwan
Hepatology 53:7-13. 2011
..99-1.00, P = 0.04). Nevertheless, the achievement of RVR was the single predictor of SVR with an OR of 19.37 (95% CI = 8.89-42.23, P < 0.001), whereas the rs8099917 genotypes played no role in achieving SVR with or without RVR...
- Genome-scale metabolic reconstruction and in silico analysis of methylotrophic yeast Pichia pastoris for strain improvement
Bevan Ks Chung
NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, 28 Medical Drive, 05 01, 117456, Singapore
Microb Cell Fact 9:50. 2010
..pastoris can be employed together with high throughput experimental data analysis, for better understanding of the methylotrophic yeast's metabolism...
- High-throughput production of human proteins for crystallization: the SGC experience
Structural Genomics Consortium, University of Oxford, Old Road Campus Research Building, Oxford, UK
J Struct Biol 172:3-13. 2010
..These can be used to formulate guidelines for initial approaches to expression of new eukaryotic proteins...
- Efficacy of boceprevir, an NS3 protease inhibitor, in combination with peginterferon alfa-2b and ribavirin in treatment-naive patients with genotype 1 hepatitis C infection (SPRINT-1): an open-label, randomised, multicentre phase 2 trial
Paul Y Kwo
Department of Medicine, Division of Gastroenterology Hepatology, Indiana University School of Medicine, Indianapolis, IN 46202 5121, USA
Lancet 376:705-16. 2010
..We tested the efficacy of boceprevir, an NS3 hepatitis C virus oral protease inhibitor, when added to peginterferon alfa-2b and ribavirin...
- Heterologous protein production using the Pichia pastoris expression system
Strathclyde Fermentation Centre, Department of Bioscience, University of Strathclyde, 204 George Street, Glasgow G1 1XW, UK
Yeast 22:249-70. 2005
..The problems associated with post-translational modifications performed on recombinant proteins by P...
- Insulin resistance impairs sustained response rate to peginterferon plus ribavirin in chronic hepatitis C patients
Hepatology Unit, Hospital Universitario de Valme, Sevilla, Spain
Gastroenterology 128:636-41. 2005
..We evaluated the effect of insulin resistance and viral factors on sustained virological response in patients with chronic hepatitis C treated with peginterferon plus ribavirin...
- Randomized study of peginterferon-alpha2a plus ribavirin vs peginterferon-alpha2b plus ribavirin in chronic hepatitis C
Maria Grazia Rumi
AM Migliavacca Center for Liver Disease, First Division of Gastroenterology, Fondazione IRCCS Maggiore Hospital, Mangiagalli e Regina Elena, Universita degli Studi di Milano, Milan, Italy
Gastroenterology 138:108-15. 2010
..Due to the lack of head-to-head studies, the 2 PegIFNs have not been directly compared. The endpoints of our study were safety and antiviral efficacy of the 2 regimens...
- Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 A resolution
R B Sutton
The Howard Hughes Medical Institute, Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
Nature 395:347-53. 1998
..These characteristics may be important for membrane fusion and for the binding of regulatory factors affecting neurotransmission...
- 2010 update of EORTC guidelines for the use of granulocyte-colony stimulating factor to reduce the incidence of chemotherapy-induced febrile neutropenia in adult patients with lymphoproliferative disorders and solid tumours
M S Aapro
Multidisciplinary Oncology Institute, Clinique de Genolier, 1, route du Muids, 1272 Genolier, Switzerland
Eur J Cancer 47:8-32. 2011
..Evidence from multiple low level studies derived from audit data and clinical practice suggests that some patients receive suboptimal daily G-CSFs; the use of pegfilgrastim may avoid this problem...
- Recombinant protein production in a variety of Nicotiana hosts: a comparative analysis
Andrew J Conley
Department of Biology, University of Western Ontario, London, ON, Canada
Plant Biotechnol J 9:434-44. 2011
..biopharmaceuticals and industrial enzymes, tobacco has the most established history for the production of recombinant proteins. To further improve the heterologous protein yield of tobacco platforms, transient and stable expression of ..
- Pre-expression of a sulfhydryl oxidase significantly increases the yields of eukaryotic disulfide bond containing proteins expressed in the cytoplasm of E.coli
Van Dat Nguyen
Department of Biochemistry, Linnanmaa campus, University of Oulu, 90570 Oulu, Finland
Microb Cell Fact 10:1. 2011
..We have recently shown that it is possible to produce large amounts of prokaryotic disulfide bond containing proteins in the cytoplasm of wild-type bacteria such as E. coli by the introduction of catalysts for both of these processes...
- Fibroblast growth factor 21 reverses hepatic steatosis, increases energy expenditure, and improves insulin sensitivity in diet-induced obese mice
Department of Metabolic Disorders, Amgen, Thousand Oaks, California, USA
Diabetes 58:250-9. 2009
..The aims of the current study are to evaluate the role of FGF21 in energy metabolism and to provide mechanistic insights into its glucose and lipid-lowering effects in a high-fat diet-induced obesity (DIO) model...
- Engineering of protein secretion in yeast: strategies and impact on protein production
R and D Group, ASPEX Division, Research Center, Asahi Glass Co, Ltd, 1150 Hazawa cho, Kanagawa ku, Yokohama, 221 8755, Japan
Appl Microbiol Biotechnol 86:403-17. 2010
..This mini-review focuses on recent strategies and their advantages for systematic engineering of yeast strains for effective protein secretion...
- Peginterferon alfa-2a plus ribavirin is more effective than peginterferon alfa-2b plus ribavirin for treating chronic hepatitis C virus infection
Department of Gastroenterology, Liver Unit, Cardarelli Hospital, Napoli, Italy
Gastroenterology 138:116-22. 2010
..This study compared the efficacy and safety of peginterferon alfa-2a and peginterferon alfa-2b, each in combination with ribavirin...
- Erythropoietin-mediated tissue protection: reducing collateral damage from the primary injury response
Warren Pharmaceuticals, Ossining, NY 10562, USA
J Intern Med 264:405-32. 2008
- Functional amyloid formation within mammalian tissue
Douglas M Fowler
Department of Chemistry, The Skaggs Institute of Chemical Biology, The Scripps Research Institute, La Jolla, California, USA
PLoS Biol 4:e6. 2006
- Vaccination against Neisseria meningitidis using three variants of the lipoprotein GNA1870
IRIS, Chiron Srl, Via Fiorentina 1, 53100 Siena, Italy
J Exp Med 197:789-99. 2003
..The novel antigen is a top candidate for the development of a new vaccine against meningococcus...
- A companion protease inhibitor for the protection of cytosol-targeted recombinant proteins in plants
CRH INAF, Pavillon des Services INAF, Universite Laval, Quebec, QC, Canada
Plant Biotechnol J 8:142-54. 2010
..potential of tomato cathepsin D inhibitor (SlCDI) as an in-built stabilizing agent for the protection of recombinant proteins in transgenic plant leaf crude extracts (Plant Biotechnol J.4, 359-368)...
- Mucosal delivery of therapeutic and prophylactic molecules using lactic acid bacteria
Jerry M Wells
Host Microbe Interactomics, University of Wageningen, Animal Sciences Department, P O Box 338, 6700 AH, Wageningen, The Netherlands
Nat Rev Microbiol 6:349-62. 2008
- Intra-arterial prourokinase for acute ischemic stroke. The PROACT II study: a randomized controlled trial. Prolyse in Acute Cerebral Thromboembolism
Cerebrovascular Center, Department of Neurology, Cleveland Clinic Foundation, Ohio 44195, USA
JAMA 282:2003-11. 1999
..Intravenous tissue-type plasminogen activator can be beneficial to some patients when given within 3 hours of stroke onset, but many patients present later after stroke onset and alternative treatments are needed...
- Cloning of a new cytokine that induces IFN-gamma production by T cells
Department of Bacteriology, Hyogo College of Medicine, Nishinomiya, Japan
Nature 378:88-91. 1995
..IGIF may be involved in the development of Th1 cells and also in mechanisms of tissue injury in inflammatory reactions...
- 10 years of the nisin-controlled gene expression system (NICE) in Lactococcus lactis
NIZO Food Research, P O Box 20, 6710, Ede, The Netherlands
Appl Microbiol Biotechnol 68:705-17. 2005
..Finally, an overview is given of growth and induction conditions for lab-scale and industrial-scale applications...
- Identification, cloning, expression, and purification of three novel human calcium-independent phospholipase A2 family members possessing triacylglycerol lipase and acylglycerol transacylase activities
Christopher M Jenkins
Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
J Biol Chem 279:48968-75. 2004
- Vaccine potential of the Neisseria meningitidis 2086 lipoprotein
Leah D Fletcher
Wyeth Vaccines Research, Pearl River, New York 10965, USA
Infect Immun 72:2088-100. 2004
..The rLP2086 family of antigens are candidates worthy of further vaccine development...
- Hepatitis C pharmacogenetics: state of the art in 2010
Nezam H Afdhal
Division of Gastroenterology and Hepatology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02215, USA
Hepatology 53:336-45. 2011
- Autophagy counterbalances endoplasmic reticulum expansion during the unfolded protein response
Howard Hughes Medical Institute, University of California San Francisco, San Francisco, California, United States of America
PLoS Biol 4:e423. 2006
..Selective ER sequestration may help cells to maintain a new steady-state level of ER abundance even in the face of continuously accumulating unfolded proteins...
- Side effects of therapy of hepatitis C and their management
Michael W Fried
Division of Digestive Diseases, University of North Carolina, Chapel Hill, North Carolina 27599, USA
Hepatology 36:S237-44. 2002
..Strategies to maximize adherence are being developed and, in the future, may include early identification of and therapy for depression and the selective use of hematopoietic growth factors to ameliorate hematologic abnormalities...
- Pivotal trial with plant cell-expressed recombinant glucocerebrosidase, taliglucerase alfa, a novel enzyme replacement therapy for Gaucher disease
Gaucher Clinic, Shaare Zedek Medical Center, Hadassah Medical School, Hebrew University, Jerusalem, Israel
Blood 118:5767-73. 2011
..4, -32.8) in the 60-unit dose group (both P < .0001); and in all secondary efficacy end point measures, except platelet counts at the lower dose. These results support safety and efficacy of taliglucerase alfa for Gaucher disease...
- Structure and function of fatty acid amide hydrolase
Michele K McKinney
Departments of Cell Biology and Chemistry, The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California 92037, USA
Annu Rev Biochem 74:411-32. 2005
..These studies, as well as their biological and therapeutic implications, are the subject of this review...
- Phase I dose-escalation study of recombinant human Apo2L/TRAIL, a dual proapoptotic receptor agonist, in patients with advanced cancer
Roy S Herbst
University of Texas M D Anderson Cancer Center, Thoracic Head and Neck Medicine Clinic, 1515 Holcombe Blvd, Unit 432, Houston, TX 77030, USA
J Clin Oncol 28:2839-46. 2010
..To the best of our knowledge, this is the first in-human clinical trial to assess the safety, tolerability, pharmacokinetics, and antitumor activity of multiple intravenous doses of rhApo2L/TRAIL in patients with advanced cancer...
- Fine mapping of the interaction of neutralizing and nonneutralizing monoclonal antibodies with the CD4 binding site of human immunodeficiency virus type 1 gp120
Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA
J Virol 77:642-58. 2003
..These reengineered gp120s are prospective immunogens that may prove capable of eliciting broadly neutralizing antibodies...
- Hepatic STAT1-nuclear translocation and interleukin 28B polymorphisms predict treatment outcomes in hepatitis C virus genotype 1-infected patients
Department of Medicine and Clinical Oncology, Graduate School of Medicine, Chiba University, Chiba, Japan
PLoS ONE 6:e28617. 2011
- Lytic activity of the virion-associated peptidoglycan hydrolase HydH5 of Staphylococcus aureus bacteriophage vB_SauS-phiIPLA88
Instituto de Productos Lacteos de Asturias IPLA CSIC, Apdo, Asturias, Spain
BMC Microbiol 11:138. 2011
..In this context, bacteriophage lytic enzymes such as endolysins and structural peptidoglycan (PG) hydrolases have received considerable attention as possible antimicrobials against gram-positive bacteria...
- Green fluorescent protein as a marker for gene expression
Department of Biological Sciences, Columbia University, New York, NY 10027
Science 263:802-5. 1994
..Because exogenous substrates and cofactors are not required for this fluorescence, GFP expression can be used to monitor gene expression and protein localization in living organisms...
- The production of recombinant pharmaceutical proteins in plants
Julian K C Ma
Department of Infectious Diseases, St George s Hospital Medical School, Cranmer Terrace, London SW17 0RE, UK
Nat Rev Genet 4:794-805. 2003
..This could one day become reality as we learn to harness the power of plants for the production of recombinant proteins on an agricultural scale...
- Peginterferon alfa-2a in patients with chronic hepatitis C
Klinikum der Johann Wolfgang Goethe Universitat, Frankfurt, Germany
N Engl J Med 343:1666-72. 2000
..We compared the clinical effects of a regimen of peginterferon alfa-2a with those of a regimen of interferon alfa-2a in the initial treatment of patients with chronic hepatitis C...
- Full-length extracellular region of the var2CSA variant of PfEMP1 is required for specific, high-affinity binding to CSA
Institut Pasteur, Unité de Biologie des Interactions Hôte Parasite, Centre National de la Recherche Scientifique Unité de Recherche Associée 2581, 25 rue du Docteur Roux, F 75724 Paris Cedex 15, France
Proc Natl Acad Sci U S A 107:4884-9. 2010
..These results have important consequences for the development of an effective vaccine and therapeutic inhibitors...
- In planta protein sialylation through overexpression of the respective mammalian pathway
Department of Applied Genetics and Cell Biology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, 1190 Vienna, Austria
J Biol Chem 285:15923-30. 2010
..Besides the biotechnological impact of the achievement, this work may serve as a general model for the manipulation of complex traits into plants...
- Full-length recombinant Plasmodium falciparum VAR2CSA binds specifically to CSPG and induces potent parasite adhesion-blocking antibodies
Department of Biochemistry, University of Cambridge, Cambridge, UK
J Mol Biol 397:826-34. 2010
..The specificity of the binding and anti-adhesion potency of induced IgG, together with high-yield production, encourages the use of full-length PfEMP1 in vaccine development strategies...
- Cloning of a disintegrin metalloproteinase that processes precursor tumour-necrosis factor-alpha
M L Moss
Department of Molecular Biochemistry, Glaxo Wellcome Research and Development Inc, Research Triangle Park, North Carolina 27709, USA
Nature 385:733-6. 1997
..The rTACE provides a readily available source of enzyme to help in the search for new anti-inflammatory agents that target the final processing stage of TNF-alpha production...
- Broad vaccine coverage predicted for a bivalent recombinant factor H binding protein based vaccine to prevent serogroup B meningococcal disease
Han Qing Jiang
Pfizer Vaccine Research, 401N Middletown Rd, Pearl River, NY 10965, USA
Vaccine 28:6086-93. 2010
..Importantly, epidemiologically prevalent fHBP variants from both subfamilies were killed...
- Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production
ARC Special Research Centre for Green Chemistry, Monash University, Building 23, Wellington Road, Clayton, Victoria 3800, Australia
J Mol Recognit 18:119-38. 2005
The use of the methylotrophic yeast, Pichia pastoris, as a cellular host for the expression of recombinant proteins has become increasing popular in recent times. P...
- Early virologic response to treatment with peginterferon alfa-2b plus ribavirin in patients with chronic hepatitis C
Gary L Davis
Division of Hepatology, Baylor University Medical Center, Dallas, TX 75246, USA
Hepatology 38:645-52. 2003
..Patients who fail to achieve EVR will not clear virus even if an additional 9 months of therapy is received. Therapy can be confidently discontinued in those cases...
- Ciliary neurotrophic factor (CNTF) for human retinal degeneration: phase I trial of CNTF delivered by encapsulated cell intraocular implants
Paul A Sieving
National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 103:3896-901. 2006
..The approach to delivering therapeutic proteins to degenerating retinas using encapsulated cell implants may have application beyond disease caused by genetic mutations...
- Inclusion bodies: a new concept
CIBER en Bioingeniería, Biomateriales y Nanomedicina, Bellaterra, 08193 Barcelona, Spain
Microb Cell Fact 9:80. 2010
..Therefore, the development of new purification protocols aimed to preserve those properties is becoming a pushing demand...
- Fibroblast growth factor 19 increases metabolic rate and reverses dietary and leptin-deficient diabetes
Genentech Inc, 1 DNA Way, South San Francisco, California 94080, USA
Endocrinology 145:2594-603. 2004
..Thus, FGF19 is able to increase metabolic rate concurrently with an increase in fatty acid oxidation...
- Biochemical characterization and ligand binding properties of neuroglobin, a novel member of the globin family
Department of Biochemistry, University of Antwerp, B 2610 Antwerp, Belgium
J Biol Chem 276:38949-55. 2001
..The biochemical and kinetic characteristics are discussed in view of the possible functions of neuroglobin in the vertebrate brain...
- Crystal structure of a mammalian voltage-dependent Shaker family K+ channel
Stephen B Long
Howard Hughes Medical Institute, Laboratory of Molecular Neurobiology and Biophysics, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
Science 309:897-903. 2005
..Electrostatic properties of the side portals and positions of the T1 domain and beta subunit are consistent with electrophysiological studies of inactivation gating and with the possibility of K+ channel regulation by the beta subunit...
- Peginterferon Alfa-2a, lamivudine, and the combination for HBeAg-positive chronic hepatitis B
George K K Lau
Department of Medicine, Queen Mary Hospital, University of Hong Kong, Hong Kong SAR, China
N Engl J Med 352:2682-95. 2005
- Soluble CD4 and CD4-mimetic compounds inhibit HIV-1 infection by induction of a short-lived activated state
Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Division of AIDS, Harvard Medical School, Boston, MA, USA
PLoS Pathog 5:e1000360. 2009
..This novel strategy for inhibition may be generally applicable to high-potential-energy viral entry machines that are normally activated by receptor binding...
- Prolonged therapy of advanced chronic hepatitis C with low-dose peginterferon
Adrian M Di Bisceglie
Division of Gastroenterology and Hepatology, Department of Internal Medicine, Saint Louis University School of Medicine, 3635 Vista Ave, St Louis, MO 63110, USA
N Engl J Med 359:2429-41. 2008
..Whether long-term antiviral therapy can prevent progressive liver disease in such patients remains uncertain...
- Generating a prion with bacterially expressed recombinant prion protein
Department of Molecular and Cellular Biochemistry, Ohio State University, Columbus, OH 43210, USA
Science 327:1132-5. 2010
..Thus, as postulated by the prion hypothesis, the infectivity in mammalian prion disease results from an altered conformation of PrP...
- Conformational changes induced in the human immunodeficiency virus envelope glycoprotein by soluble CD4 binding
Q J Sattentau
Academic Department of Genito Urinary Medicine, University College and Middlesex School of Medicine, London, United Kingdom
J Exp Med 174:407-15. 1991
..We propose that these events occurring after CD4 binding are integral components of the membrane fusion reaction between HIV or HIV-infected cells and CD4+ cells...
- The Anopheles gambiae odorant binding protein 1 (AgamOBP1) mediates indole recognition in the antennae of female mosquitoes
Developmental Biology Center, Department of Molecular Biology and Biochemistry, University of California Irvine, Irvine, California, United States of America
PLoS ONE 5:e9471. 2010
..gambiae OBP-ligand pairing combination, demonstrates the significance of OBPs in odor recognition, and can be expanded to the identification of other ligands for OBPs of Anopheles and other medically important insects...
- Ultrasensitive detection of scrapie prion protein using seeded conversion of recombinant prion protein
Laboratory of Persistent Viral Diseases, National Institutes of Health, 903 S 4th Street, Hamilton, Montana 59840, USA
Nat Methods 4:645-50. 2007
..This method should facilitate the development of rapid, ultrasensitive prion assays and diagnostic tests, in addition to aiding fundamental studies of structure and mechanism of PrPSc formation...
- The metabolic state of diabetic monkeys is regulated by fibroblast growth factor-21
Lilly Research Laboratories, A Division of Eli Lilly and Company, Indianapolis, Indiana 46285, USA
Endocrinology 148:774-81. 2007
..These data support the development of FGF-21 for the treatment of diabetes and other metabolic diseases...
- Characterization of an Arabidopsis enzyme family that conjugates amino acids to indole-3-acetic acid
Paul E Staswick
Department of Agronomy and Horticulture, University of Nebraska, Lincoln, Nebraska 68583, USA
Plant Cell 17:616-27. 2005
..This evidence establishes that several GH3 genes encode IAA-amido synthetases, which help to maintain auxin homeostasis by conjugating excess IAA to amino acids...
- Human SIR2 deacetylates p53 and antagonizes PML/p53-induced cellular senescence
Wellcome Institute Cancer Research UK and Department of Pathology, University of Cambridge, Tennis Court Road, CB2 1QR, UK
EMBO J 21:2383-96. 2002
..Taken together, our data establish the SIRT1 deacetylase as a novel negative regulator of p53 function capable of modulating cellular senescence...
- Importance of IL28B gene polymorphisms in hepatitis C virus genotype 2 and 3 infected patients
Medizinische Klinik 1, J W Goethe University Hospital, Frankfurt am Main, Germany
J Hepatol 54:415-21. 2011
..The importance of three IL28B single nucleotide polymorphisms (rs8099917, rs12980275 and rs12979860) for HCV genotype 2/3-infected patients is unknown...
- The alarmin HMGB1 acts in synergy with endogenous and exogenous danger signals to promote inflammation
Hulda Sigridur Hreggvidsdottir
Department of Medicine, Rheumatology Research Unit, Karolinska Hospital, Karolinska Institutet, S 171 76 Stockholm, Sweden
J Leukoc Biol 86:655-62. 2009
..HMGB1 thus acts broadly with many but not all immunostimulatory molecules to amplify their activity in a synergistic manner...
- Inherent toxicity of aggregates implies a common mechanism for protein misfolding diseases
Dipartimento di Scienze Biochimiche, Viale Morgagni 50, Universita degli Studi di Firenze, 50134 Firenze, Italy
Nature 416:507-11. 2002
..This finding provides added evidence that avoidance of protein aggregation is crucial for the preservation of biological function and suggests common features in the origins of this family of protein deposition diseases...
- Low micromolar zinc accelerates the fibrillization of human tau via bridging of Cys-291 and Cys-322
Zhong Ying Mo
State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China
J Biol Chem 284:34648-57. 2009
- Single domain camel antibodies: current status
Vrije Universiteit Brussel, Vlaams Interuniversitair Instituut voor Biotechnologie, Sint Genesius Rode, Belgium
J Biotechnol 74:277-302. 2001
..They are expected to open perspectives as enzyme inhibitors and intrabodies, as modular building units for multivalent or multifunctional constructs, or as immuno-adsorbents and detection units in biosensors...
- Frequency of factor H-binding protein modular groups and susceptibility to cross-reactive bactericidal activity in invasive meningococcal isolates
Center for Immunobiology and Vaccine Development, Children s Hospital Oakland Research Institute, 5700 Martin Luther King Jr Way, Oakland, CA 94609, USA
Vaccine 28:2122-9. 2010
..The results confirmed the modular architecture of fHbp and underscored its importance for the design of broadly protective group B vaccines in different regions...
- Micelle-associated protein in epoetin formulations: aA risk factor for immunogenicity?
Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences UIPS, Utrecht, The Netherlands
Pharm Res 20:1903-7. 2003
..The aim of this study was to detect the mechanism by which the new formulation may induce these antibodies...
- Bone morphogenetic protein-1: the type I procollagen C-proteinase
Maurice and Gabriela Goldschleger Eye Research Institute, Tel Aviv University Sackler Faculty of Medicine, Sheba Medical Center, Tel Hashomer, Israel
Science 271:360-2. 1996
..Here it is shown that BMP-1 and PCP are identical. This demonstration of enzymatic activity for a BMP-1/TLD-like protein links an enzyme involved in matrix deposition to genes involved in pattern formation...
- High efficacy of anti DBL4ɛ-VAR2CSA antibodies in inhibition of CSA-binding Plasmodium falciparum-infected erythrocytes from pregnant women
Pamela A Magistrado
Centre for Medical Parasitology, Department of International Health, Immunology and Microbiology, University of Copenhagen, Denmark
Vaccine 29:437-43. 2011
..In this study, the binding inhibitory efficacy of IgG elicited by two different DBL4ɛ recombinant proteins was tested on a panel of fresh clinical isolates from pregnant women living in Benin and Tanzania...
- Tyrosine phosphorylation of focal adhesion kinase at sites in the catalytic domain regulates kinase activity: a role for Src family kinases
M B Calalb
Department of Cell Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232
Mol Cell Biol 15:954-63. 1995
..Our results indicate that phosphorylation of FAK by Src (or other Src family kinases) is an important step in the formation of an active signaling complex...
- Transgenic chickens as bioreactors for protein-based drugs
Simon G Lillico
Roslin Institute, Roslin, Midlothian EH25 9PS, UK
Drug Discov Today 10:191-6. 2005
- Quantitative characterization of heparin binding to Tau protein: implication for inducer-mediated Tau filament formation
Hai Li Zhu
State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China
J Biol Chem 285:3592-9. 2010
- An APAF-1.cytochrome c multimeric complex is a functional apoptosome that activates procaspase-9
Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas 75235, USA
J Biol Chem 274:11549-56. 1999
..The stoichiometric ratio of procaspase-9 to APAF-1 is approximately 1 to 1 in the complex. Once activated, caspase-9 disassociates from the complex and becomes available to cleave and activate downstream caspases such as caspase-3...
- Cloning, expression and characterization of gE protein of duck plague virus
Avian Diseases Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Yaan, Sichuan, China
Virol J 7:120. 2010
..The gE protein of duck plague virus is the important membrane glycoprotein, its protein characterization has not been reported. In this study, we expressed and presented the characterization of the DPV gE product...
- Structure and ligand of a histone acetyltransferase bromodomain
Structural Biology Program, Department of Physiology and Biophysics, Mount Sinai School of Medicine, New York, New York 10029 6574, USA
Nature 399:491-6. 1999
..Thus, the bromodomain is functionally linked to the HAT activity of co-activators in the regulation of gene transcription...