tropoelastin

Summary

Summary: A salt-soluble precursor of elastin. Lysyl oxidase is instrumental in converting it to elastin in connective tissue.

Top Publications

  1. ncbi Serum factors from pseudoxanthoma elasticum patients alter elastic fiber formation in vitro
    Olivier Le Saux
    Department of Cell and Molecular Biology, John A Burns School of Medicine, University of Hawaii, Honolulu, Hawaii, USA
    J Invest Dermatol 126:1497-505. 2006
  2. pmc New insights into the pathogenesis of autosomal-dominant cutis laxa with report of five ELN mutations
    Bert Callewaert
    Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium
    Hum Mutat 32:445-55. 2011
  3. ncbi Structural determinants of cross-linking and hydrophobic domains for self-assembly of elastin-like polypeptides
    Ming Miao
    Research Institute, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada
    Biochemistry 44:14367-75. 2005
  4. ncbi Dissection of human tropoelastin: exon-by-exon chemical synthesis and related conformational studies
    Antonio M Tamburro
    Department of Chemistry, Universita della Basilicata, Via N Sauro 85, 85100 Potenza, Italy
    Biochemistry 42:13347-62. 2003
  5. ncbi Sequences and domain structures of mammalian, avian, amphibian and teleost tropoelastins: Clues to the evolutionary history of elastins
    Martin I S Chung
    Cardiovascular Research Program, Research Institute, Hospital for Sick Children, Toronto, ON, Canada M5G1X8
    Matrix Biol 25:492-504. 2006
  6. doi Kinetics and morphology of self-assembly of an elastin-like polypeptide based on the alternating domain arrangement of human tropoelastin
    Judith T Cirulis
    Molecular Structure and Function Program, Research Institute, The Hospital for Sick Children, and Department of Biochemistry, University of Toronto, 555 University Avenue, Toronto, Ontario, Canada M5G1X8
    Biochemistry 49:5726-33. 2010
  7. ncbi An elastin gene mutation producing abnormal tropoelastin and abnormal elastic fibres in a patient with autosomal dominant cutis laxa
    M Tassabehji
    University Department of Medical Genetics and Regional Genetics Service, St Mary s Hospital, Manchester M13 0JH, UK
    Hum Mol Genet 7:1021-8. 1998
  8. pmc Fibulin-4 regulates expression of the tropoelastin gene and consequent elastic-fibre formation by human fibroblasts
    Qiuyun Chen
    Department of Molecular Cardiology, Lerner Research Institute, Cleveland Clinic, OH 44195, USA
    Biochem J 423:79-89. 2009
  9. doi The immobilization of recombinant human tropoelastin on metals using a plasma-activated coating to improve the biocompatibility of coronary stents
    Anna Waterhouse
    School of Molecular Bioscience, University of Sydney, NSW 2006, Australia
    Biomaterials 31:8332-40. 2010
  10. ncbi Structural changes and facilitated association of tropoelastin
    Lisa D Muiznieks
    School of Molecular and Microbial Biosciences G08, University of Sydney, Sydney, New South Wales 2006, Australia
    Arch Biochem Biophys 410:317-23. 2003

Detail Information

Publications199 found, 100 shown here

  1. ncbi Serum factors from pseudoxanthoma elasticum patients alter elastic fiber formation in vitro
    Olivier Le Saux
    Department of Cell and Molecular Biology, John A Burns School of Medicine, University of Hawaii, Honolulu, Hawaii, USA
    J Invest Dermatol 126:1497-505. 2006
    ..The expression of tropoelastin and other elastic fiber-associated genes was not significantly modulated by the presence of PXE serum...
  2. pmc New insights into the pathogenesis of autosomal-dominant cutis laxa with report of five ELN mutations
    Bert Callewaert
    Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium
    Hum Mutat 32:445-55. 2011
    ..Mutant protein expression in fibroblast cultures impaired deposition of tropoelastin onto microfibril-containing fibers, and enhanced tropoelastin coacervation and globule formation leading to ..
  3. ncbi Structural determinants of cross-linking and hydrophobic domains for self-assembly of elastin-like polypeptides
    Ming Miao
    Research Institute, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada
    Biochemistry 44:14367-75. 2005
    ..To achieve the required structural durability of the elastic matrix, the elastin monomer, tropoelastin, undergoes ordered assembly into a covalently cross-linked, fibrillar polymeric structure...
  4. ncbi Dissection of human tropoelastin: exon-by-exon chemical synthesis and related conformational studies
    Antonio M Tamburro
    Department of Chemistry, Universita della Basilicata, Via N Sauro 85, 85100 Potenza, Italy
    Biochemistry 42:13347-62. 2003
    Polypeptide sequences encoding the single exons of human tropoelastin were synthesized and their conformations were studied in different solvents and at different temperatures by CD and (1)H NMR...
  5. ncbi Sequences and domain structures of mammalian, avian, amphibian and teleost tropoelastins: Clues to the evolutionary history of elastins
    Martin I S Chung
    Cardiovascular Research Program, Research Institute, Hospital for Sick Children, Toronto, ON, Canada M5G1X8
    Matrix Biol 25:492-504. 2006
    b>Tropoelastin is the monomeric form of elastin, a polymeric extracellular matrix protein responsible for properties of extensibility and elastic recoil in connective tissues of most vertebrates...
  6. doi Kinetics and morphology of self-assembly of an elastin-like polypeptide based on the alternating domain arrangement of human tropoelastin
    Judith T Cirulis
    Molecular Structure and Function Program, Research Institute, The Hospital for Sick Children, and Department of Biochemistry, University of Toronto, 555 University Avenue, Toronto, Ontario, Canada M5G1X8
    Biochemistry 49:5726-33. 2010
    ..Both tropoelastin (TE), the full-length monomeric form of elastin, and elastin-like polypeptides (ELPs), based on sequences and ..
  7. ncbi An elastin gene mutation producing abnormal tropoelastin and abnormal elastic fibres in a patient with autosomal dominant cutis laxa
    M Tassabehji
    University Department of Medical Genetics and Regional Genetics Service, St Mary s Hospital, Manchester M13 0JH, UK
    Hum Mol Genet 7:1021-8. 1998
    ..These findings suggest that the mutant tropoelastin protein is synthesized, secreted and incorporated into the elastic matrix, where it alters the architecture of ..
  8. pmc Fibulin-4 regulates expression of the tropoelastin gene and consequent elastic-fibre formation by human fibroblasts
    Qiuyun Chen
    Department of Molecular Cardiology, Lerner Research Institute, Cleveland Clinic, OH 44195, USA
    Biochem J 423:79-89. 2009
    ..analysis showed that knockdown of fibulin-4 expression was accompanied by reduced expression of tropoelastin mRNA. Further analysis showed that this decrease was caused by transcriptional down-regulation of tropoelastin...
  9. doi The immobilization of recombinant human tropoelastin on metals using a plasma-activated coating to improve the biocompatibility of coronary stents
    Anna Waterhouse
    School of Molecular Bioscience, University of Sydney, NSW 2006, Australia
    Biomaterials 31:8332-40. 2010
    ..We previously demonstrated a plasma-activated coating (PAC) that covalently bound recombinant human tropoelastin (TE), a major regulator of vascular cells in vivo, to enhance endothelial cell interactions...
  10. ncbi Structural changes and facilitated association of tropoelastin
    Lisa D Muiznieks
    School of Molecular and Microbial Biosciences G08, University of Sydney, Sydney, New South Wales 2006, Australia
    Arch Biochem Biophys 410:317-23. 2003
    Circular dichroism studies of tropoelastin secondary structure show 4+/-1% alpha-helix in aqueous solutions...
  11. ncbi Differential expression of two tropoelastin genes in zebrafish
    M Miao
    Cardiovascular Research, Hospital for Sick Children, Toronto, ON, Canada
    Matrix Biol 26:115-24. 2007
    ..Elastin is synthesized as a monomer, tropoelastin, but is rapidly transformed into its final polymeric form in the extracellular matrix...
  12. doi Molecular orientation of tropoelastin is determined by surface hydrophobicity
    Anton P Le Brun
    Bragg Institute, Australian Nuclear Science and Technology Organisation, Locked Bag 2001, Kirrawee DC, NSW 2232, Australia
    Biomacromolecules 13:379-86. 2012
    b>Tropoelastin is the precursor of the extracellular protein elastin and is utilized in tissue engineering and implant technology by adapting the interface presented by surface-bound tropoelastin...
  13. doi Coacervation of tropoelastin
    Giselle C Yeo
    School of Molecular Bioscience, The University of Sydney, Australia
    Adv Colloid Interface Sci 167:94-103. 2011
    The coacervation of tropoelastin represents the first major stage of elastic fiber assembly...
  14. doi Characterization of an unusual tropoelastin with truncated C-terminus in the frog
    Ming Miao
    Molecular Structure and Function Program, Hospital for Sick Children, Toronto, Canada
    Matrix Biol 28:432-41. 2009
    b>Tropoelastin is the monomeric form of elastin, a major polymeric protein of the extracellular elastic matrix of vertebrate tissues with properties of extensibility and elastic recoil...
  15. doi Tropoelastin as a thermodynamically unfolded premolten globule protein: The effect of trimethylamine N-oxide on structure and coacervation
    Leanne B Dyksterhuis
    CSIRO Molecular and Health Technologies, Clayton, VIC 3168, Australia
    Arch Biochem Biophys 487:79-84. 2009
    b>Tropoelastin is the monomer building block of the biopolymer elastin, which is responsible for elasticity in arteries, lung and skin...
  16. pmc Biomaterials derived from silk-tropoelastin protein systems
    Xiao Hu
    Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA
    Biomaterials 31:8121-31. 2010
    A structural protein blend system based on silkworm silk fibroin and recombinant human tropoelastin is described...
  17. doi Degradation of tropoelastin by matrix metalloproteinases--cleavage site specificities and release of matrikines
    Andrea Heinz
    Institute of Pharmacy, Martin Luther University Halle Wittenberg, Halle Saale, Germany
    FEBS J 277:1939-56. 2010
    ..elastin-degrading diseases, the aim of this study was to investigate the degradation of the natural substrate tropoelastin by the elastinolytic matrix metalloproteinases MMP-7, MMP-9, and MMP-12 and to compare the cleavage site ..
  18. pmc Shape of tropoelastin, the highly extensible protein that controls human tissue elasticity
    Clair Baldock
    Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, United Kingdom
    Proc Natl Acad Sci U S A 108:4322-7. 2011
    Elastin enables the reversible deformation of elastic tissues and can withstand decades of repetitive forces. Tropoelastin is the soluble precursor to elastin, the main elastic protein found in mammals...
  19. ncbi The dissection of human tropoelastin: from the molecular structure to the self-assembly to the elasticity mechanism
    Antonio M Tamburro
    Department of Chemistry, Universita della Basilicata, Potenza, Italy
    Pathol Biol (Paris) 53:383-9. 2005
    ..results obtained by analyzing the conformation of polypeptide sequences encoded by the single exons of human tropoelastin demonstrated the presence of labile conformations such as poly-proline II helix (PPII) and beta-turns whose ..
  20. pmc Tropoelastin bridge region positions the cell-interactive C terminus and contributes to elastic fiber assembly
    Giselle C Yeo
    School of Molecular Bioscience, University of Sydney, New South Wales 2006, Australia
    Proc Natl Acad Sci U S A 109:2878-83. 2012
    The tropoelastin monomer undergoes stages of association by coacervation, deposition onto microfibrils, and cross-linking to form elastic fibers...
  21. ncbi Elastase-released epidermal growth factor recruits epidermal growth factor receptor and extracellular signal-regulated kinases to down-regulate tropoelastin mRNA in lung fibroblasts
    Sandra J DiCamillo
    Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    J Biol Chem 277:18938-46. 2002
    ..with transient, peaking at 30 min, induction of c-Fos and sustained, observed at 24-48 h, decrease of tropoelastin mRNA levels in NE-challenged cells...
  22. pmc Oxidative modifications of the C-terminal domain of tropoelastin prevent cell binding
    Kamal Akhtar
    Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 286:13574-82. 2011
    b>Tropoelastin (TE), the soluble monomer of elastin, is synthesized by elastogenic cells, such as chondrocytes, fibroblasts, and smooth muscle cells (SMCs)...
  23. ncbi Localizing alpha-helices in human tropoelastin: assembly of the elastin "puzzle"
    Antonio Mario Tamburro
    Department of Chemistry, Universita degli Studi della Basilicata, Via N Sauro, 85, 85100 Potenza, Italy
    Biochemistry 45:9518-30. 2006
    Polyalanine cross-linking domains encoded by exons 6, 15, 17, 19, 21, 23, 25, 27, 29, 31 of human tropoelastin were synthesized, and their conformations were studied in different solutions and at different temperatures by CD and (1)H NMR...
  24. doi New insights into elastic fiber assembly
    Jessica E Wagenseil
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Birth Defects Res C Embryo Today 81:229-40. 2007
    ....
  25. ncbi Fibulin-5 mutations: mechanisms of impaired elastic fiber formation in recessive cutis laxa
    Qirui Hu
    Department of Pediatrics, Washington University School of Medicine, St Louis, MO 63110, USA
    Hum Mol Genet 15:3379-86. 2006
    ..Purified recombinant fibulin-5 with either mutation showed reduced affinity for tropoelastin in solid-phase binding assays...
  26. ncbi Ultraviolet radiation increases tropoelastin mRNA expression in the epidermis of human skin in vivo
    J Y Seo
    Department of Dermatology, Seoul National University College of Medicine, Seoul National University Hospital, Korea
    J Invest Dermatol 116:915-9. 2001
    ..In this study, it was demonstrated that ultraviolet irradiation induced tropoelastin mRNA expression in the keratinocytes of human skin in vivo and also in cultured human keratinocytes by in situ ..
  27. pmc Analysis of dermal elastic fibers in the absence of fibulin-5 reveals potential roles for fibulin-5 in elastic fiber assembly
    Jiwon Choi
    Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada
    Matrix Biol 28:211-20. 2009
    ..lower levels of mature cross-linked elastin in the Fbln5(-/-) dermis, however, gene expression levels for tropoelastin and fibrillin-1, the major elastic fiber components, were unaffected...
  28. ncbi Molecular interactions of biglycan and decorin with elastic fiber components: biglycan forms a ternary complex with tropoelastin and microfibril-associated glycoprotein 1
    Betty Reinboth
    Department of Pathology, University of Adelaide, Adelaide, South Australia 5005, Australia
    J Biol Chem 277:3950-7. 2002
    ..dermatan sulfate proteoglycans biglycan and decorin have been investigated with the elastic fiber components, tropoelastin, fibrillin-containing microfibrils, and microfibril-associated glycoproteins (MAGP) 1 and 2...
  29. ncbi Elastic fiber formation: a dynamic view of extracellular matrix assembly using timer reporters
    Beth A Kozel
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Cell Physiol 207:87-96. 2006
    ..the dynamics of elastic fiber assembly, mammalian cells were transfected with a cDNA construct encoding bovine tropoelastin in frame with the Timer reporter...
  30. doi DANCE/fibulin-5 promotes elastic fiber formation in a tropoelastin isoform-dependent manner
    Risa Nonaka
    Department of Clinical Chemistry, Hoshi University School of Pharmacy and Pharmaceutical Sciences, Tokyo, Japan
    Clin Biochem 42:713-21. 2009
    ..of fibulin-5 in the elastic fiber formation, we studied the molecular interactions among elastin, fibrillin-1, and fibulin-5 in the extracellular space and the maturation of tropoelastin using retinal pigment epithelial cells (ARPE-19).
  31. ncbi Tropoelastin binding to fibulins, nidogen-2 and other extracellular matrix proteins
    T Sasaki
    Max Planck Institut fur Biochemie, Am Klopferspitz 18a, D 82152, Martinsried, Germany
    FEBS Lett 460:280-4. 1999
    Elastic fibers in vessel walls and other tissues consist of cross-linked tropoelastin in association with several microfibrillar proteins...
  32. pmc EGF antagonizes TGF-beta-induced tropoelastin expression in lung fibroblasts via stabilization of Smad corepressor TGIF
    Shenghong Yang
    Department of Biochemistry, Boston University School of Medicine, 715 Albany St, Boston, Massachusetts 02118, USA
    Am J Physiol Lung Cell Mol Physiol 295:L143-51. 2008
    ..reported that neutrophil elastase (NE) downregulates transforming growth factor-beta (TGF-beta)-maintained tropoelastin mRNA levels in lung fibroblasts through transactivation of the epidermal growth factor (EGF) receptor (EGFR)/..
  33. doi In vitro degradation of human tropoelastin by MMP-12 and the generation of matrikines from domain 24
    Samuel Taddese
    Institute of Pharmacy, Martin Luther University Halle Wittenberg, Halle Saale, Germany
    Matrix Biol 28:84-91. 2009
    ..b>Tropoelastin is the precursor of the cross-linked elastin and its expression is triggered by elastin-degrading factors as a ..
  34. ncbi Tropoelastin massively associates during coacervation to form quantized protein spheres
    Adam W Clarke
    School of Molecular and Microbial Biosciences G08, University of Sydney, New South Wales 2006, Australia
    Biochemistry 45:9989-96. 2006
    b>Tropoelastin, the precursor of elastin, undergoes a rapid monomer to multimer association in an inverse temperature transition. This association culminates in the rapid formation of stable, optically distinct droplets of tropoelastin...
  35. ncbi Gene expression and accumulation of fibrillin-1, fibrillin-2, and tropoelastin in cultured periodontal fibroblasts
    E Tsuruga
    Department of Oral Anatomy, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari Tobetsu, Hokkaido 061 0293, Japan
    J Dent Res 81:771-5. 2002
    ..to determine the gene expression of fibrillin-1 and fibrillin-2 (the major components of microfibrils) and of tropoelastin. In addition, we assessed the degree of accumulation of these proteins in the extracellular matrix...
  36. ncbi Tropoelastin interacts with cell-surface glycosaminoglycans via its COOH-terminal domain
    Thomas J Broekelmann
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 280:40939-47. 2005
    ..and cell biological approach, we have identified a cell interaction site at the carboxyl terminus of tropoelastin. Cell interactions with the COOH-terminal sequence are not through the elastin-binding protein (EBP67) because ..
  37. pmc Repeated domains of leptospira immunoglobulin-like proteins interact with elastin and tropoelastin
    Yi Pin Lin
    Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, New York, USA
    J Biol Chem 284:19380-91. 2009
    ..In this study, we report that Leptospira can be immobilized by human tropoelastin (HTE) or elastin from different tissues, including lung, skin, and blood vessels, and that Lig proteins can ..
  38. doi Heparan sulfate regulates fibrillin-1 N- and C-terminal interactions
    Stuart A Cain
    Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, United Kingdom
    J Biol Chem 283:27017-27. 2008
    ..by exons 59-62 near the fibrillin-1 C terminus are novel conformation-dependent high affinity heparin and tropoelastin binding sites...
  39. ncbi Fibrillin-1 interactions with heparin. Implications for microfibril and elastic fiber assembly
    Stuart A Cain
    Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, United Kingdom
    J Biol Chem 280:30526-37. 2005
    ..cell attachment, but heparin and MAGP-1 competed for binding to the fibrillin-1 N terminus, and heparin and tropoelastin competed for binding to a central fibrillin-1 sequence...
  40. ncbi Coacervation is promoted by molecular interactions between the PF2 segment of fibrillin-1 and the domain 4 region of tropoelastin
    Adam W Clarke
    School of Molecular and Microbial Biosciences G08, University of Sydney, Sydney, New South Wales, 2006, Australia
    Biochemistry 44:10271-81. 2005
    In forming elastic fibers, microfibrils act as the scaffold sites for depositing the elastin precursor tropoelastin. We examined key binding interactions that promote massive tropoelastin association through coacervation...
  41. ncbi Autosomal dominant cutis laxa with severe lung disease: synthesis and matrix deposition of mutant tropoelastin
    Zsolt Urban
    Department of Anatomy, University of Hawaii, Honolulu, Hawaii, USA
    J Invest Dermatol 124:1193-9. 2005
    ..In addition to the normal 68 kDa tropoelastin (TE) protein, an abnormal, 120 kDa polypeptide was detected in the proband and her affected daughter in a CL ..
  42. ncbi Protein interaction studies of MAGP-1 with tropoelastin and fibrillin-1
    S A Jensen
    Department of Biochemistry G08, University of Sydney, New South Wales 2006, Australia
    J Biol Chem 276:39661-6. 2001
    ..To investigate the interaction of MAGP-1 with tropoelastin and fibrillin-1, we expressed human MAGP-1 as a T7-tag fusion protein in Escherichia coli...
  43. ncbi The Pro-regions of lysyl oxidase and lysyl oxidase-like 1 are required for deposition onto elastic fibers
    Laetitia Thomassin
    Institut de Biologie et Chimie des Proteines, CNRS Université Lyon 1 UMR 5086, 7 passage du Vercors, 69367 Lyon Cedex 07, France
    J Biol Chem 280:42848-55. 2005
    ..Ligand blot and mammalian two-hybrid assays confirmed an interaction between tropoelastin and the pro-regions of both LOX and LOXL...
  44. pmc Targeted disruption of fibulin-4 abolishes elastogenesis and causes perinatal lethality in mice
    Precious J McLaughlin
    Department of Ophthalmology and Vision Science, University of Arizona, Tucson, AZ 85711, USA
    Mol Cell Biol 26:1700-9. 2006
    ..However, expression of tropoelastin or lysyl oxidase mRNA was unaffected in fibulin-4-/- mice...
  45. pmc Loss-of-function mutations in ATP6V0A2 impair vesicular trafficking, tropoelastin secretion and cell survival
    Vishwanathan Hucthagowder
    Department of Pediatrics, Washington University School of Medicine, 660 South Euclid Avenue, Campus Box 8208, St Louis, MO 63110, USA
    Hum Mol Genet 18:2149-65. 2009
    ..Immunostaining of ARCL2 cells showed the accumulation of tropoelastin (TE) in the Golgi and in large, abnormal intracellular and extracellular aggregates...
  46. ncbi Supramolecular amyloid-like assembly of the polypeptide sequence coded by exon 30 of human tropoelastin
    Antonio Mario Tamburro
    Department of Chemistry, Universita della Basilicata, Via N Sauro 85, 85100 Potenza, Italy
    J Biol Chem 280:2682-90. 2005
    ..similar to those shown by amyloid networks, is shown by the polypeptide sequence encoded by exon 30 of human tropoelastin. To better understand the molecular properties of this sequence to give amyloid fibers, we used CD, NMR, and ..
  47. ncbi Microfibril-associated glycoprotein-1 and fibrillin-2 are associated with tropoelastin deposition in vitro
    Eichi Tsuruga
    Department of Oral Anatomy, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari Tobetsu, Hokkaido 061 0293, Japan
    Int J Biochem Cell Biol 37:120-9. 2005
    Elastic system fibers consist of microfibrils and tropoelastin. During development, microfibrils act as a template on which tropoelastin is deposited...
  48. doi Fibrillins, fibulins, and matrix-associated glycoprotein modulate the kinetics and morphology of in vitro self-assembly of a recombinant elastin-like polypeptide
    Judith T Cirulis
    Research Institute, The Hospital for Sick Children, Toronto, Canada
    Biochemistry 47:12601-13. 2008
    ..Recent data suggest the complex interaction of tropoelastin, the monomeric form of elastin, with a number of other elastic matrix-associated proteins, including fibrillins,..
  49. ncbi Sustained changes in lung expansion alter tropoelastin mRNA levels and elastin content in fetal sheep lungs
    Belinda J Joyce
    Fetal and Neonatal Research Group, Department of Physiology, Monash University, Victoria 3800, Australia
    Am J Physiol Lung Cell Mol Physiol 284:L643-9. 2003
    ..Lung tropoelastin mRNA levels were assessed by Northern blot analysis, total elastin content was measured biochemically, and ..
  50. ncbi Molecular basis of elastic fiber formation. Critical interactions and a tropoelastin-fibrillin-1 cross-link
    Matthew J Rock
    Wellcome Trust Centre for Cell Matrix Research, School of Biological Sciences, University of Manchester, 2 205 Stopford Building, Oxford Road, Manchester M13 9PT, United Kingdom
    J Biol Chem 279:23748-58. 2004
    ..binding of two overlapping fibrillin-1 fragments (encoded by central exons 18-25 and 24-30) to tropoelastin, which, in microfibrils, map to an exposed "arms" feature adjacent to the beads...
  51. ncbi The cysteine residues in the carboxy terminal domain of tropoelastin form an intrachain disulfide bond that stabilizes a loop structure and positively charged pocket
    P L Brown
    Department of Cell Biology, Jewish Hospital, Washington University Medical Center, St Louis, MO 63110
    Biochem Biophys Res Commun 186:549-55. 1992
    Analysis of purified bovine tropoelastin with Ellman's reagent and [14C]iodoacetamide demonstrated that the only two cysteine residues in the molecule form an intrachain disulfide bond...
  52. doi Characterization of the molecular interaction between tropoelastin and DANCE/fibulin-5
    Hiroshi Wachi
    Department of Clinical Chemistry, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2 4 41 Ebara, Shinagawa ku, Tokyo 142 8501, Japan
    J Biochem 143:633-9. 2008
    ..The present experiments were carried out to characterize the molecular interaction between fibulin-5 and tropoelastin. Our data showed that the divalent cations of Ca(2+), Ba(2+) and Mg(2+) significantly enhanced the binding of ..
  53. ncbi Distinct steps of cross-linking, self-association, and maturation of tropoelastin are necessary for elastic fiber formation
    Fumiaki Sato
    Department of Clinical Chemistry, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2 4 41 Ebara, Shinagawa ku, Tokyo 142 8501, Japan
    J Mol Biol 369:841-51. 2007
    Elastic fibers play an important role in the characteristic resilience of many tissues. The assembly of tropoelastin into a fibrillar matrix is a complex stepwise process and the deposition and cross-linking of tropoelastin are believed ..
  54. ncbi A comparative analysis of the fibulin protein family. Biochemical characterization, binding interactions, and tissue localization
    Naoyuki Kobayashi
    Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
    J Biol Chem 282:11805-16. 2007
    ..Binding to tropoelastin was strong for fibulin-2 and -5, moderate for fibulin-4 and -1, and relatively weak for fibulin-3...
  55. pmc Fibulin-5/DANCE has an elastogenic organizer activity that is abrogated by proteolytic cleavage in vivo
    Maretoshi Hirai
    Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    J Cell Biol 176:1061-71. 2007
    ..We report that fibulin-5 protein potently induces elastic fiber assembly and maturation by organizing tropoelastin and cross-linking enzymes onto microfibrils...
  56. ncbi Interaction of tropoelastin with the amino-terminal domains of fibrillin-1 and fibrillin-2 suggests a role for the fibrillins in elastic fiber assembly
    T M Trask
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 275:24400-6. 2000
    Alignment of tropoelastin molecules during the process of elastogenesis is thought to require fibrillin-containing microfibrils...
  57. pmc Identification of tropoelastin as a ligand for the 65-kD FK506-binding protein, FKBP65, in the secretory pathway
    E C Davis
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Cell Biol 140:295-303. 1998
    The folding and trafficking of tropoelastin is thought to be mediated by intracellular chaperones, although the identity and role of any tropoelastin chaperone remain to be determined...
  58. ncbi Sequential loss of two neighboring exons of the tropoelastin gene during primate evolution
    Z Szabo
    Pacific Biomedical Research Center, University of Hawaii, Honolulu, HI 96822, USA
    J Mol Evol 49:664-71. 1999
    Previous evidence has demonstrated the absence of exons 34 and 35 within the 3' end of the human tropoelastin (ELN) gene...
  59. ncbi Fibroblast tropoelastin and alpha-smooth-muscle actin expression are repressed by particulate-activated macrophage-derived tumor necrosis factor-alpha in experimental silicosis
    T J Mariani
    Department of Internal Medicine, Washington University School of Medicine at Barnes Jewish Hospital, St Louis, Missouri, USA
    Am J Respir Cell Mol Biol 21:185-92. 1999
    ..Reinitiated tropoelastin expression was identified throughout the lung in fibroblasts expressing alpha-smooth-muscle actin, whereas ..
  60. ncbi Fibrillin-1 interactions with fibulins depend on the first hybrid domain and provide an adaptor function to tropoelastin
    Ehab El-Hallous
    Faculty of Medicine, Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec H3A 2B2, Canada
    J Biol Chem 282:8935-46. 2007
    ..Formation of ternary complexes between fibrillin-1, fibulins, and tropoelastin demonstrated that fibulin-2 and -5 but much less fibulin-4, are able to act as molecular adaptors between ..
  61. ncbi Retrovirally mediated overexpression of versican v3 by arterial smooth muscle cells induces tropoelastin synthesis and elastic fiber formation in vitro and in neointima after vascular injury
    Mervyn J Merrilees
    Department of Anatomy with Radiology, The University of Auckland, Auckland, New Zealand
    Circ Res 90:481-7. 2002
    ..We now report that V3-overexpressing ASMCs exhibit significantly increased expression of tropoelastin and increased formation of elastic fibers in long-term cell cultures...
  62. doi Glycosaminoglycan-mediated coacervation of tropoelastin abolishes the critical concentration, accelerates coacervate formation, and facilitates spherule fusion: implications for tropoelastin microassembly
    Yidong Tu
    School of Molecular and Microbial Biosciences G08, University of Sydney, NSW 2006, Australia
    Biomacromolecules 9:1739-44. 2008
    Elastogenesis and elastin repair depend on the secretion of tropoelastin from the cell, yet cellular production is low in the many biological systems that have been studied...
  63. ncbi Building Elastin. Incorporation of recombinant human tropoelastin into extracellular matrices using nonelastogenic rat-1 fibroblasts as a source for lysyl oxidase
    P J Stone
    Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    Am J Respir Cell Mol Biol 24:733-9. 2001
    The purpose of this study was to assess the feasibility of crosslinking exogenously produced tropoelastin, the precursor of insoluble elastin, into existing elastin...
  64. ncbi Hydrophobic domains of human tropoelastin interact in a context-dependent manner
    P Toonkool
    Department of Biochemistry, University of Sydney, Sydney, New South Wales 2006, Australia
    J Biol Chem 276:44575-80. 2001
    b>Tropoelastin is the soluble precursor of elastin, the major component of the extracellular elastic fiber...
  65. ncbi Tropoelastin expression by periodontal fibroblasts
    E Tsuruga
    Department of Oral Anatomy, School of Dentistry, Health Sciences University of Hokkaido, Ishikari Tobetsu, Japan
    J Dent Res 81:198-202. 2002
    ..We examined gene expression and accumulation of tropoelastin in the cell-matrix layers of human gingival fibroblasts (HGF) and periodontal ligament fibroblasts (HPLF) in ..
  66. ncbi Heparan sulphate interacts with tropoelastin, with some tropoelastin peptides and is present in human dermis elastic fibers
    Dealba Gheduzzi
    Department of Biomedical Sciences, University of Modena and Reggio Emilia, Via Campi 287, 41100 Modena, Italy
    Matrix Biol 24:15-25. 2005
    ..with normal elastic fibers in human dermis and that isolated HS chains interact in vitro with recombinant tropoelastin and with peptides encoded by distinct exons of the human tropoelastin gene (EDPs)...
  67. ncbi Development of a new in vitro model of elastic fiber assembly in human pigmented epithelial cells
    Hiroshi Wachi
    Department of Clinical Chemistry, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2 4 41 Ebara, Shinagawa ku, Tokyo 142 8501, Japan
    Clin Biochem 38:643-53. 2005
    We developed an in vitro model of elastic fiber assembly that provides a comparison of the efficiency of different tropoelastin molecules to organize into fibers.
  68. pmc Decreased elastin deposition and high proliferation of fibroblasts from Costello syndrome are related to functional deficiency in the 67-kD elastin-binding protein
    A Hinek
    Division of Cardiovascular Research, The Hospital for Sick Children, Toronto, ON M5G 1X8 Canada
    Am J Hum Genet 66:859-72. 2000
    ..obtained from patients with Costello syndrome did not assemble elastic fibers, despite an adequate synthesis of tropoelastin and normal deposition of the microfibrillar scaffold...
  69. ncbi Action of tropoelastin and synthetic elastin sequences on vascular tone and on free Ca2+ level in human vascular endothelial cells
    G Faury
    Laboratoire de Bioénergétique Fondamentale et Appliquée, Universite Joseph Fourier, Grenoble, France
    Circ Res 82:328-36. 1998
    ..we have compared the action of different concentrations (10(-13) to 10(-7) mol/L) of recombinant human tropoelastin, eight synthetic elastin peptides, and a control peptide (VPVGGA) on both rat aortic ring tension and [Ca2+]i ..
  70. ncbi Fibrillin-1 and fibrillin-2 show temporal and tissue-specific regulation of expression in developing elastic tissues
    M C Mariencheck
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Connect Tissue Res 31:87-97. 1995
    ..these possibilities, the expression of fibrillin-1 and fibrillin-2 was compared with expression of MAGP and tropoelastin in two elastogenic tissues that undergo different developmental programs...
  71. pmc Domains 16 and 17 of tropoelastin in elastic fibre formation
    Hiroshi Wachi
    Department of Clinical Chemistry, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2 4 41 Ebara, Shinagawa ku, Tokyo 142 8501, Japan
    Biochem J 402:63-70. 2007
    ..b>Tropoelastin lacking domains 16-17 (Delta16-17) was synthesized efficiently and secreted by transfected retinal pigment ..
  72. ncbi Gene expression of tropoelastin is enhanced in the aorta proximal to the coarctation in rabbits
    Chengpei Xu
    Department of Surgery, Stanford University, Division of Vascular Surgery, 300 Pasteur Drive, Suite H3600, Stanford, California, USA
    Exp Mol Pathol 72:115-23. 2002
    ..in the aortic wall in response to acute elevation of blood pressure, we studied the aortic gene expression of tropoelastin in a rabbit midthoracic aortic coarctation model...
  73. pmc Retrovirally mediated overexpression of glycosaminoglycan-deficient biglycan in arterial smooth muscle cells induces tropoelastin synthesis and elastic fiber formation in vitro and in neointimae after vascular injury
    Jin Yong Hwang
    Hope Heart Program, Benaroya Research Institute, Seattle, WA 98101, USA
    Am J Pathol 173:1919-28. 2008
    ..Cultured cells overexpressing LmBSN showed marked up-regulation of tropoelastin and fibulin-5 mRNAs, increased amounts of desmosine and insoluble elastin, and increased deposition of elastic ..
  74. ncbi Heterogeneity of rat tropoelastin mRNA revealed by cDNA cloning
    R A Pierce
    Department of Surgery, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, New Brunswick 08903 0019
    Biochemistry 29:9677-83. 1990
    ..gt11 library constructed from poly(A+) RNA isolated from aortic tissue of neonatal rats was screened for rat tropoelastin cDNAs. The first screen, utilizing a human tropoelastin cDNA clone, provided rat tropoelastin cDNAs spanning 2...
  75. ncbi Expression of fibrillins and tropoelastin by human gingival and periodontal ligament fibroblasts in vitro
    Eichi Tsuruga
    Department of Oral Anatomy, School of Dentistry, Health Sciences University of Hokkaido, Ishikari Tobetsu, Japan
    J Periodontal Res 37:23-8. 2002
    ..the major components of microfibrils involved in elastogenesis, we investigated the synthesis of fibrillins and tropoelastin in the conditioned medium of HGF and HPLF...
  76. ncbi Use of an intron polymorphism to localize the tropoelastin gene to mouse chromosome 5 in a region of linkage conservation with human chromosome 7
    K S Wydner
    Department of Biological Sciences, Rutgers University, Piscataway, New Jersey 08855 1059
    Genomics 23:125-31. 1994
    The complete coding sequence for mouse tropoelastin was obtained from overlapping reverse transcriptase polymerase chain reaction (PCR) amplimers...
  77. ncbi Induction of fibulin-5 gene is regulated by tropoelastin gene, and correlated with tropoelastin accumulation in vitro
    Eichi Tsuruga
    Department of Oral Anatomy, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari Tobetsu, Hokkaido 061 0293, Japan
    Int J Biochem Cell Biol 36:395-400. 2004
    ..examined the relationship between the gene expression of fibulin-5 and the gene expression and accumulation of tropoelastin by comparing elastin-producing cells (human gingival fibroblasts) with non-elastin-producing cells (human ..
  78. ncbi Investigating the amyloidogenic nanostructured sequences of elastin: sequence encoded by exon 28 of human tropoelastin gene
    Brigida Bochicchio
    Department of Chemistry, Universita della Basilicata, Via N Sauro 85, Potenza, Italy
    Biomacromolecules 8:3478-86. 2007
    In this paper we demonstrate that the sequence encoded by exon 28 (EX28) of human tropoelastin gene is able to give amyloid-like fibrils...
  79. ncbi Granulocyte macrophage colony-stimulating factor deficiency affects vascular elastin production and integrity of elastic lamellae
    Gabriele Weissen-Plenz
    Leibniz Institute for Arteriosclerosis Research, Munster, Germany
    J Vasc Res 45:103-10. 2008
    ..The present study elaborates the effect of GM-CSF deficiency on the vascular elastin system...
  80. ncbi Locally generated VGVAPG and VAPG elastin-derived peptides amplify melanoma invasion via the galectin-3 receptor
    Peter Pocza
    Department of Genetics, Cell, and Immunbiology, Semmelweis University, Nagyvarad ter 4, Budapest H 1089, Hungary
    Int J Cancer 122:1972-80. 2008
    ..Elastin, tropoelastin and VGVAPG peptide were demonstrated at the invasion site of melanoma using histochemistry and ..
  81. ncbi Elastic fiber macro-assembly is a hierarchical, cell motion-mediated process
    Andras Czirok
    Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA
    J Cell Physiol 207:97-106. 2006
    ..It was recently demonstrated that in vitro, cells first secrete tropoelastin into a punctate pattern of globules...
  82. pmc VE-statin/egfl7 regulates vascular elastogenesis by interacting with lysyl oxidases
    Etienne Lelievre
    CNRS, UMR 8161, Institut de Biologie de Lille, Equipe labellisée La Ligue 2005, Universite de Lille I, Universite de Lille II, Institut Pasteur de Lille, Lille, France
    EMBO J 27:1658-70. 2008
    ..the presence of VE-statin/egfl7 were unable to deposit elastic fibres due to a deficient conversion of soluble tropoelastin into insoluble mature elastin...
  83. ncbi Significant decrease in tropoelastin gene expression in fibroblasts from a Japanese Costello syndrome patient with impaired elastogenesis and enhanced proliferation
    Yutaka Tatano
    Department of Medicinal Biotechnology, Institute for Medicinal Resources, Graduate School of Pharmaceutical Sciences, The University of Tokushima, Tokushima 770 8505
    J Biochem 140:193-200. 2006
    ..In this study we demonstrated a significant decrease in the expression of tropoelastin mRNA in fibroblasts derived from a Japanese Costello syndrome patient with impaired elastogenesis and enhanced ..
  84. ncbi Disordered pulmonary myofibroblast distribution and elastin expression in preterm infants with Ureaplasma urealyticum pneumonitis
    Rose Viscardi
    Department of Pediatrics, University of Maryland School of Medicine, 22 South Greene Street, Baltimore, 21201, USA
    Pediatr Dev Pathol 9:143-51. 2006
    ..and transforming growth factor beta1 (TGFbeta1), Hart's elastin staining, and in situ hybridization for tropoelastin (TE) expression...
  85. doi High copper levels and increased elastolysis in a patient with cutis marmorata teleangiectasia congenita
    Aleksander Hinek
    Division of Cardiovascular Research, The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada
    Am J Med Genet A 146:2520-7. 2008
    ..cultures (passage one) of dermal fibroblasts derived from this patient were capable of normal synthesis of tropoelastin, but their net deposition of mature elastic fibers was significantly diminished as compared with cultures of ..
  86. ncbi Ellagic and tannic acids protect newly synthesized elastic fibers from premature enzymatic degradation in dermal fibroblast cultures
    Felipe Jimenez
    Research Department, Human Matrix Sciences, LLC, Visalia, California, USA
    J Invest Dermatol 126:1272-80. 2006
    ..Results of a pulse and chase experiment showed that pretreatment with both polyphenols enhanced biostability of tropoelastin and newly deposited elastin...
  87. ncbi Regulation of elastin promoter by lysyl oxidase and growth factors: cross control of lysyl oxidase on TGF-beta1 effects
    Roberta Oleggini
    Laboratorio di Nefrologia, Istituto G Gaslini, Genova, Italy
    Matrix Biol 26:494-505. 2007
    ..Many of those included SMAD transcription factors. Two more restricted regions binding AP1 and SMAD were identified as mediators of LOX effects and of LOX and TGF-beta1 cross-inhibition...
  88. doi Mapping of macrophage elastase cleavage sites in insoluble human skin elastin
    Samuel Taddese
    Institute of Pharmacy, Martin Luther University Halle Wittenberg, Halle Saale, Germany
    Matrix Biol 27:420-8. 2008
    ..The amino acid preferences of the different subsites on the catalytic domain of MMP-12 were analyzed...
  89. pmc Aldosterone induces elastin production in cardiac fibroblasts through activation of insulin-like growth factor-I receptors in a mineralocorticoid receptor-independent manner
    Severa Bunda
    Cardiovascular Research Program, University of Toronto, Ontario, Canada
    Am J Pathol 171:809-19. 2007
    ..Most importantly, we demonstrated that aldosterone also increases elastin mRNA levels, tropoelastin synthesis, and elastic fiber deposition in a dose-dependent manner...
  90. ncbi Elastogenesis in human arterial disease: a role for macrophages in disordered elastin synthesis
    Alexandra Krettek
    Leducq Center for Cardiovascular Research, Cardiovascular Division, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, 221 Longwood Avenue, Eugene Braunwald Research Center 307, Boston, Mass 02115, USA
    Arterioscler Thromb Vasc Biol 23:582-7. 2003
    ..However, elastogenesis in arterial diseases has received little attention. In this work we hypothesized that disordered elastogenesis is active in matrix remodeling in atheroma and abdominal aortic aneurysm (AAA)...
  91. ncbi Spectroscopic evidence revealing polyproline II structure in hydrophobic, putatively elastomeric sequences encoded by specific exons of human tropoelastin
    Brigida Bochicchio
    Laboratoire de Spectroscopies et Structures BioMoléculaires, LSSBM EA 3305, Universite de Reims Champagne Ardenne, IFR 53 Biomolecules, UFR Sciences Exactes et Naturelles, BP 1039, 51687 Reims Cedex 2, France
    Biopolymers 73:484-93. 2004
    ..Given the cassette like organization of the human tropoelastin gene, giving rise to alternating exons encoding for crosslink domains and elastomeric domains, it is tempting ..
  92. ncbi ECM gene expression correlates with in vitro tissue growth and development in fibrin gel remodeled by neonatal smooth muscle cells
    J J Ross
    Departments of Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455, USA
    Matrix Biol 22:477-90. 2003
    ..Following fibrin gel contraction during week 1, peak rates of SMC proliferation, collagen production and tropoelastin production occurred between weeks 1-4...
  93. ncbi Elastin induces myofibrillogenesis via a specific domain, VGVAPG
    Satyajit K Karnik
    Program in Human Molecular Biology and Genetics and Department of Oncological Sciences, University of Utah, 15 North 2030 East Rm 4450, Salt Lake City, UT 84112 5330, USA
    Matrix Biol 22:409-25. 2003
    ..Previously, we showed that the Elastin gene product, tropoelastin, activates myofibrillar organization of VSMCs...
  94. ncbi Elastin: relation of protein and gene structure to disease
    J Rosenbloom
    Lab Invest 51:605-23. 1984
    ..studies and in vitro translation of elastin mRNA have demonstrated that a 72,000-dalton polypeptide, designated tropoelastin, is the initial translation product...
  95. ncbi Domains in tropoelastin that mediate elastin deposition in vitro and in vivo
    Beth A Kozel
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 278:18491-8. 2003
    ..To identify domains in the tropoelastin molecule important for the assembly process, we utilized an in vitro assembly model to map sequences within ..
  96. ncbi Nature and the multiple functions of the 67-kD elastin-/laminin binding protein
    A Hinek
    Division of Cardiovascular Research, Hospital for Sick Children, Toronto, Canada
    Cell Adhes Commun 2:185-93. 1994
    ..I will emphasize the role of the 67-kD protein in divergent cellular processes, such as cell-matrix attachment, matrix assembly, cellular chemotaxis, and active migration through the vascular walls...
  97. pmc Functional domains on elastin and microfibril-associated glycoprotein involved in elastic fibre assembly
    P Brown-Augsburger
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, MO 63110, USA
    Biochem J 318:149-55. 1996
    Studies in vitro suggest that the C-terminus of tropoelastin mediates elastin polymerization through an interaction with microfibril-associated proteins...
  98. pmc Impaired elastic-fiber assembly by fibroblasts from patients with either Morquio B disease or infantile GM1-gangliosidosis is linked to deficiency in the 67-kD spliced variant of beta-galactosidase
    A Hinek
    Division of Cardiovascular Research, The Hospital for Sick Children, Toronto, Ontario, Canada
    Am J Hum Genet 67:23-36. 2000
    We have previously shown that intracellular trafficking and extracellular assembly of tropoelastin into elastic fibers is facilitated by the 67-kD elastin-binding protein identical to an enzymatically inactive, alternatively spliced ..
  99. ncbi Elastic fibers: building bridges between cells and their matrix
    Kim S Midwood
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544 1014, USA
    Curr Biol 12:R279-81. 2002
    ..Extracellular elastic fibers confer resilience and flexibility to tissues. Recent studies have identified a protein, fibulin-5, that connects these fibers to cells and regulates their assembly and organization...
  100. ncbi [Effect of elastin peptides on the production of matrix metalloproteinase 2 by human skin fibroblasts in culture]
    E Huet
    Signalisation Cellulaire et Matrice Extracellulaire, CNRS FRE 2260, , , , Reims, France
    J Soc Biol 195:165-72. 2001
    Soluble elastin-derived peptides from alkaline or elastase hydrolysis of insoluble elastin, as well as tropoelastin, increase matrix metalloproteinase-2 (MMP-2) production by human skin fibroblasts in culture as determined by gelatin ..
  101. ncbi Nitric oxide stimulates elastin expression in chick aortic smooth muscle cells
    H Sugitani
    Department of Clinical Chemistry, Hoshi College of Pharmacy, Tokyo, Japan
    Biol Pharm Bull 24:461-4. 2001
    ..S-Nitrosoglutathione (GSNO), a NO donor, stimulated tropoelastin synthesis in cultured SMCs during both the quiescent and proliferating phases...

Research Grants74

  1. INTRACELLULAR TRAFFICKING OF TROPOELASTIN
    Elaine Davis; Fiscal Year: 2001
    Elastic fiber assembly is a complicated process that involves the organization of tropoelastin monomers on a microfibril scaffold and the subsequent crosslinking of these monomers into an insoluble elastin matrix...
  2. Cues for cell-mediated regeneration of elastin matrix to stabilize aortic aneurys
    Anand Ramamurthi; Fiscal Year: 2010
    ..wherein RASMCs (healthy and aneurysmal) will be provided cues to both synthesize, and assemble and crosslink tropoelastin precursors...
  3. Cues for cell-mediated regeneration of elastin matrix to stabilize aortic aneurys
    Anand Ramamurthi; Fiscal Year: 2009
    ..wherein RASMCs (healthy and aneurysmal) will be provided cues to both synthesize, and assemble and crosslink tropoelastin precursors...
  4. Cues for cell-mediated regeneration of elastin matrix to stabilize aortic aneurys
    Anand Ramamurthi; Fiscal Year: 2009
    ..wherein RASMCs (healthy and aneurysmal) will be provided cues to both synthesize, and assemble and crosslink tropoelastin precursors...
  5. INTRACELLULAR TRAFFICKING OF TROPOELASTIN
    Frederick Grinnell; Fiscal Year: 2002
    Elastic fiber assembly is a complicated process that involves the organization of tropoelastin monomers on a microfibril scaffold and the subsequent crosslinking of these monomers into an insoluble elastin matrix...
  6. FIBULIN-5 & WALL STRESSES IN VASCULAR REMODELING: THEORY AND EX VIVO EXPERIMENTS
    RUDOLPH GLEASON; Fiscal Year: 2007
    ..Fibulin-5 is an ECM protein that binds tropoelastin and fibrillins with ava3, ava5, and a9a1 integrins(60) to bridge elastic fibers with cells...
  7. Disorganization of Elastin Matrix Mediates Erectile Dysfunction
    KANCHAN A CHITALEY; Fiscal Year: 2010
    ..By maturity, assembly of elastic fibers is complete, and synthesis of new tropoelastin, the soluble precursor, has declined...
  8. Elastin Gene Mutations: Mechanisms Causing SVAS and ADCL
    Zsolt Urban; Fiscal Year: 2005
    ..In ADCL patients, in contrast, we have identified mutations that result in the expression of mutant tropoelastin. Based on these results, we hypotesize that different classes of elastin gene mutations result in SVAS and ADCL ..
  9. EXTRACELLULAR MATRIX REPAIR IN PULMONARY EMPHYSEMA
    Steven Shapiro; Fiscal Year: 2002
    ..Elastic fiber assembly is complex requiring tropoelastin monomers to be aligned on a scaffold of potentially several microfibril proteins where they are then cross-..
  10. Regulation of Vascular Wall Extracellular Matrix
    William Parks; Fiscal Year: 2006
    ..Elastin is a covalently crosslinked polymer of tropoelastin, the secreted precursor protein, which are assembled into fibers on a microfibrillar scaffold...
  11. Bioengineering & Biologic Studies of Aneurysm Weakening
    David Vorp; Fiscal Year: 2009
    ..to either degradation or maintenance of wall integrity, such as matrix metalloproteinases and ECM precursors tropoelastin and procollagen. The results of this study could have an immediate impact on the clinical management of AAA...
  12. Functional Repair of Elastin with Recomb. Tropoelastin
    Phillip Stone; Fiscal Year: 2004
    ..this goal our objectives are to regenerate elastin in tissue culture systems by crosslinking recombinant tropoelastin (rTE) into damaged elastin and to characterize the effectiveness of this regeneration by assessing the ..
  13. MOLECULAR BIOLOGY OF THE MARFAN SYNDROME
    Harry Dietz; Fiscal Year: 2004
    ..The prevailing view has been that microfibrils regulate the deposition of tropoelastin in late fetal life...
  14. MECHANISMS FOR CHRONIC UVR-INDUCED DAMAGE TO SKIN
    IRENE KOCHEVAR; Fiscal Year: 2003
    ..The processes involved in stimulating synthesis of tropoelastin by fibroblasts in response to UVR are only inadequately understood...
  15. POSTTRANSCRIPTIONAL REGULATION OF TROPOELASTIN EXPRESSIO
    William Parks; Fiscal Year: 2001
    ..Elastin, the principal component of elastic fibers, is the product of crosslinked tropoelastin monomers...