tropoelastin

Summary

Summary: A salt-soluble precursor of elastin. Lysyl oxidase is instrumental in converting it to elastin in connective tissue.

Top Publications

  1. ncbi Serum factors from pseudoxanthoma elasticum patients alter elastic fiber formation in vitro
    Olivier Le Saux
    Department of Cell and Molecular Biology, John A Burns School of Medicine, University of Hawaii, Honolulu, Hawaii, USA
    J Invest Dermatol 126:1497-505. 2006
  2. ncbi Fibulin-5 mutations: mechanisms of impaired elastic fiber formation in recessive cutis laxa
    Qirui Hu
    Department of Pediatrics, Washington University School of Medicine, St Louis, MO 63110, USA
    Hum Mol Genet 15:3379-86. 2006
  3. ncbi Molecular interactions of biglycan and decorin with elastic fiber components: biglycan forms a ternary complex with tropoelastin and microfibril-associated glycoprotein 1
    Betty Reinboth
    Department of Pathology, University of Adelaide, Adelaide, South Australia 5005, Australia
    J Biol Chem 277:3950-7. 2002
  4. ncbi Tropoelastin massively associates during coacervation to form quantized protein spheres
    Adam W Clarke
    School of Molecular and Microbial Biosciences G08, University of Sydney, New South Wales 2006, Australia
    Biochemistry 45:9989-96. 2006
  5. ncbi Heparan sulphate interacts with tropoelastin, with some tropoelastin peptides and is present in human dermis elastic fibers
    Dealba Gheduzzi
    Department of Biomedical Sciences, University of Modena and Reggio Emilia, Via Campi 287, 41100 Modena, Italy
    Matrix Biol 24:15-25. 2005
  6. ncbi The Pro-regions of lysyl oxidase and lysyl oxidase-like 1 are required for deposition onto elastic fibers
    Laetitia Thomassin
    Institut de Biologie et Chimie des Proteines, CNRS Université Lyon 1 UMR 5086, 7 passage du Vercors, 69367 Lyon Cedex 07, France
    J Biol Chem 280:42848-55. 2005
  7. ncbi Interaction of tropoelastin with the amino-terminal domains of fibrillin-1 and fibrillin-2 suggests a role for the fibrillins in elastic fiber assembly
    T M Trask
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 275:24400-6. 2000
  8. ncbi Retrovirally mediated overexpression of versican v3 by arterial smooth muscle cells induces tropoelastin synthesis and elastic fiber formation in vitro and in neointima after vascular injury
    Mervyn J Merrilees
    Department of Anatomy with Radiology, The University of Auckland, Auckland, New Zealand
    Circ Res 90:481-7. 2002
  9. ncbi New insights into elastic fiber assembly
    Jessica E Wagenseil
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Birth Defects Res C Embryo Today 81:229-40. 2007
  10. ncbi Decreased elastin deposition and high proliferation of fibroblasts from Costello syndrome are related to functional deficiency in the 67-kD elastin-binding protein
    A Hinek
    Division of Cardiovascular Research, The Hospital for Sick Children, Toronto, ON M5G 1X8 Canada
    Am J Hum Genet 66:859-72. 2000

Research Grants

  1. INTRACELLULAR TRAFFICKING OF TROPOELASTIN
    Elaine Davis; Fiscal Year: 2001
  2. Cues for cell-mediated regeneration of elastin matrix to stabilize aortic aneurys
    Anand Ramamurthi; Fiscal Year: 2010
  3. Cues for cell-mediated regeneration of elastin matrix to stabilize aortic aneurys
    Anand Ramamurthi; Fiscal Year: 2009
  4. Cues for cell-mediated regeneration of elastin matrix to stabilize aortic aneurys
    Anand Ramamurthi; Fiscal Year: 2009
  5. INTRACELLULAR TRAFFICKING OF TROPOELASTIN
    Frederick Grinnell; Fiscal Year: 2002
  6. FIBULIN-5 & WALL STRESSES IN VASCULAR REMODELING: THEORY AND EX VIVO EXPERIMENTS
    RUDOLPH GLEASON; Fiscal Year: 2007
  7. Disorganization of Elastin Matrix Mediates Erectile Dysfunction
    KANCHAN A CHITALEY; Fiscal Year: 2010
  8. Elastin Gene Mutations: Mechanisms Causing SVAS and ADCL
    Zsolt Urban; Fiscal Year: 2005
  9. EXTRACELLULAR MATRIX REPAIR IN PULMONARY EMPHYSEMA
    Steven Shapiro; Fiscal Year: 2002
  10. Regulation of Vascular Wall Extracellular Matrix
    William Parks; Fiscal Year: 2006

Detail Information

Publications183 found, 100 shown here

  1. ncbi Serum factors from pseudoxanthoma elasticum patients alter elastic fiber formation in vitro
    Olivier Le Saux
    Department of Cell and Molecular Biology, John A Burns School of Medicine, University of Hawaii, Honolulu, Hawaii, USA
    J Invest Dermatol 126:1497-505. 2006
    ..The expression of tropoelastin and other elastic fiber-associated genes was not significantly modulated by the presence of PXE serum...
  2. ncbi Fibulin-5 mutations: mechanisms of impaired elastic fiber formation in recessive cutis laxa
    Qirui Hu
    Department of Pediatrics, Washington University School of Medicine, St Louis, MO 63110, USA
    Hum Mol Genet 15:3379-86. 2006
    ..Purified recombinant fibulin-5 with either mutation showed reduced affinity for tropoelastin in solid-phase binding assays...
  3. ncbi Molecular interactions of biglycan and decorin with elastic fiber components: biglycan forms a ternary complex with tropoelastin and microfibril-associated glycoprotein 1
    Betty Reinboth
    Department of Pathology, University of Adelaide, Adelaide, South Australia 5005, Australia
    J Biol Chem 277:3950-7. 2002
    ..dermatan sulfate proteoglycans biglycan and decorin have been investigated with the elastic fiber components, tropoelastin, fibrillin-containing microfibrils, and microfibril-associated glycoproteins (MAGP) 1 and 2...
  4. ncbi Tropoelastin massively associates during coacervation to form quantized protein spheres
    Adam W Clarke
    School of Molecular and Microbial Biosciences G08, University of Sydney, New South Wales 2006, Australia
    Biochemistry 45:9989-96. 2006
    b>Tropoelastin, the precursor of elastin, undergoes a rapid monomer to multimer association in an inverse temperature transition. This association culminates in the rapid formation of stable, optically distinct droplets of tropoelastin...
  5. ncbi Heparan sulphate interacts with tropoelastin, with some tropoelastin peptides and is present in human dermis elastic fibers
    Dealba Gheduzzi
    Department of Biomedical Sciences, University of Modena and Reggio Emilia, Via Campi 287, 41100 Modena, Italy
    Matrix Biol 24:15-25. 2005
    ..with normal elastic fibers in human dermis and that isolated HS chains interact in vitro with recombinant tropoelastin and with peptides encoded by distinct exons of the human tropoelastin gene (EDPs)...
  6. ncbi The Pro-regions of lysyl oxidase and lysyl oxidase-like 1 are required for deposition onto elastic fibers
    Laetitia Thomassin
    Institut de Biologie et Chimie des Proteines, CNRS Université Lyon 1 UMR 5086, 7 passage du Vercors, 69367 Lyon Cedex 07, France
    J Biol Chem 280:42848-55. 2005
    ..Ligand blot and mammalian two-hybrid assays confirmed an interaction between tropoelastin and the pro-regions of both LOX and LOXL...
  7. ncbi Interaction of tropoelastin with the amino-terminal domains of fibrillin-1 and fibrillin-2 suggests a role for the fibrillins in elastic fiber assembly
    T M Trask
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 275:24400-6. 2000
    Alignment of tropoelastin molecules during the process of elastogenesis is thought to require fibrillin-containing microfibrils...
  8. ncbi Retrovirally mediated overexpression of versican v3 by arterial smooth muscle cells induces tropoelastin synthesis and elastic fiber formation in vitro and in neointima after vascular injury
    Mervyn J Merrilees
    Department of Anatomy with Radiology, The University of Auckland, Auckland, New Zealand
    Circ Res 90:481-7. 2002
    ..We now report that V3-overexpressing ASMCs exhibit significantly increased expression of tropoelastin and increased formation of elastic fibers in long-term cell cultures...
  9. ncbi New insights into elastic fiber assembly
    Jessica E Wagenseil
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Birth Defects Res C Embryo Today 81:229-40. 2007
    ....
  10. ncbi Decreased elastin deposition and high proliferation of fibroblasts from Costello syndrome are related to functional deficiency in the 67-kD elastin-binding protein
    A Hinek
    Division of Cardiovascular Research, The Hospital for Sick Children, Toronto, ON M5G 1X8 Canada
    Am J Hum Genet 66:859-72. 2000
    ..obtained from patients with Costello syndrome did not assemble elastic fibers, despite an adequate synthesis of tropoelastin and normal deposition of the microfibrillar scaffold...
  11. ncbi Characterization of the molecular interaction between tropoelastin and DANCE/fibulin-5
    Hiroshi Wachi
    Department of Clinical Chemistry, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2 4 41 Ebara, Shinagawa ku, Tokyo 142 8501, Japan
    J Biochem 143:633-9. 2008
    ..The present experiments were carried out to characterize the molecular interaction between fibulin-5 and tropoelastin. Our data showed that the divalent cations of Ca(2+), Ba(2+) and Mg(2+) significantly enhanced the binding of ..
  12. ncbi Distinct steps of cross-linking, self-association, and maturation of tropoelastin are necessary for elastic fiber formation
    Fumiaki Sato
    Department of Clinical Chemistry, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2 4 41 Ebara, Shinagawa ku, Tokyo 142 8501, Japan
    J Mol Biol 369:841-51. 2007
    Elastic fibers play an important role in the characteristic resilience of many tissues. The assembly of tropoelastin into a fibrillar matrix is a complex stepwise process and the deposition and cross-linking of tropoelastin are believed ..
  13. ncbi Targeted disruption of fibulin-4 abolishes elastogenesis and causes perinatal lethality in mice
    Precious J McLaughlin
    Department of Ophthalmology and Vision Science, University of Arizona, Tucson, AZ 85711, USA
    Mol Cell Biol 26:1700-9. 2006
    ..However, expression of tropoelastin or lysyl oxidase mRNA was unaffected in fibulin-4-/- mice...
  14. ncbi Sustained changes in lung expansion alter tropoelastin mRNA levels and elastin content in fetal sheep lungs
    Belinda J Joyce
    Fetal and Neonatal Research Group, Department of Physiology, Monash University, Victoria 3800, Australia
    Am J Physiol Lung Cell Mol Physiol 284:L643-9. 2003
    ..Lung tropoelastin mRNA levels were assessed by Northern blot analysis, total elastin content was measured biochemically, and ..
  15. ncbi Building Elastin. Incorporation of recombinant human tropoelastin into extracellular matrices using nonelastogenic rat-1 fibroblasts as a source for lysyl oxidase
    P J Stone
    Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    Am J Respir Cell Mol Biol 24:733-9. 2001
    The purpose of this study was to assess the feasibility of crosslinking exogenously produced tropoelastin, the precursor of insoluble elastin, into existing elastin...
  16. ncbi Protein interaction studies of MAGP-1 with tropoelastin and fibrillin-1
    S A Jensen
    Department of Biochemistry G08, University of Sydney, New South Wales 2006, Australia
    J Biol Chem 276:39661-6. 2001
    ..To investigate the interaction of MAGP-1 with tropoelastin and fibrillin-1, we expressed human MAGP-1 as a T7-tag fusion protein in Escherichia coli...
  17. ncbi Hydrophobic domains of human tropoelastin interact in a context-dependent manner
    P Toonkool
    Department of Biochemistry, University of Sydney, Sydney, New South Wales 2006, Australia
    J Biol Chem 276:44575-80. 2001
    b>Tropoelastin is the soluble precursor of elastin, the major component of the extracellular elastic fiber...
  18. ncbi A comparative analysis of the fibulin protein family. Biochemical characterization, binding interactions, and tissue localization
    Naoyuki Kobayashi
    Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
    J Biol Chem 282:11805-16. 2007
    ..Binding to tropoelastin was strong for fibulin-2 and -5, moderate for fibulin-4 and -1, and relatively weak for fibulin-3...
  19. ncbi Gene expression and accumulation of fibrillin-1, fibrillin-2, and tropoelastin in cultured periodontal fibroblasts
    E Tsuruga
    Department of Oral Anatomy, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari Tobetsu, Hokkaido 061 0293, Japan
    J Dent Res 81:771-5. 2002
    ..to determine the gene expression of fibrillin-1 and fibrillin-2 (the major components of microfibrils) and of tropoelastin. In addition, we assessed the degree of accumulation of these proteins in the extracellular matrix...
  20. ncbi Tropoelastin expression by periodontal fibroblasts
    E Tsuruga
    Department of Oral Anatomy, School of Dentistry, Health Sciences University of Hokkaido, Ishikari Tobetsu, Japan
    J Dent Res 81:198-202. 2002
    ..We examined gene expression and accumulation of tropoelastin in the cell-matrix layers of human gingival fibroblasts (HGF) and periodontal ligament fibroblasts (HPLF) in ..
  21. ncbi Sequential loss of two neighboring exons of the tropoelastin gene during primate evolution
    Z Szabo
    Pacific Biomedical Research Center, University of Hawaii, Honolulu, HI 96822, USA
    J Mol Evol 49:664-71. 1999
    Previous evidence has demonstrated the absence of exons 34 and 35 within the 3' end of the human tropoelastin (ELN) gene...
  22. ncbi Tropoelastin interacts with cell-surface glycosaminoglycans via its COOH-terminal domain
    Thomas J Broekelmann
    Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri 63110, USA
    J Biol Chem 280:40939-47. 2005
    ..and cell biological approach, we have identified a cell interaction site at the carboxyl terminus of tropoelastin. Cell interactions with the COOH-terminal sequence are not through the elastin-binding protein (EBP67) because ..
  23. ncbi Fibrillins, fibulins, and matrix-associated glycoprotein modulate the kinetics and morphology of in vitro self-assembly of a recombinant elastin-like polypeptide
    Judith T Cirulis
    Research Institute, The Hospital for Sick Children, Toronto, Canada
    Biochemistry 47:12601-13. 2008
    ..Recent data suggest the complex interaction of tropoelastin, the monomeric form of elastin, with a number of other elastic matrix-associated proteins, including fibrillins,..
  24. ncbi Fibulin-5/DANCE has an elastogenic organizer activity that is abrogated by proteolytic cleavage in vivo
    Maretoshi Hirai
    Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    J Cell Biol 176:1061-71. 2007
    ..We report that fibulin-5 protein potently induces elastic fiber assembly and maturation by organizing tropoelastin and cross-linking enzymes onto microfibrils...
  25. ncbi In vitro degradation of human tropoelastin by MMP-12 and the generation of matrikines from domain 24
    Samuel Taddese
    Institute of Pharmacy, Martin Luther University Halle Wittenberg, Halle Saale, Germany
    Matrix Biol 28:84-91. 2009
    ..b>Tropoelastin is the precursor of the cross-linked elastin and its expression is triggered by elastin-degrading factors as a ..
  26. ncbi Supramolecular amyloid-like assembly of the polypeptide sequence coded by exon 30 of human tropoelastin
    Antonio Mario Tamburro
    Department of Chemistry, Universita della Basilicata, Via N Sauro 85, 85100 Potenza, Italy
    J Biol Chem 280:2682-90. 2005
    ..similar to those shown by amyloid networks, is shown by the polypeptide sequence encoded by exon 30 of human tropoelastin. To better understand the molecular properties of this sequence to give amyloid fibers, we used CD, NMR, and ..
  27. ncbi Loss-of-function mutations in ATP6V0A2 impair vesicular trafficking, tropoelastin secretion and cell survival
    Vishwanathan Hucthagowder
    Department of Pediatrics, Washington University School of Medicine, 660 South Euclid Avenue, Campus Box 8208, St Louis, MO 63110, USA
    Hum Mol Genet 18:2149-65. 2009
    ..Immunostaining of ARCL2 cells showed the accumulation of tropoelastin (TE) in the Golgi and in large, abnormal intracellular and extracellular aggregates...
  28. ncbi Repeated domains of leptospira immunoglobulin-like proteins interact with elastin and tropoelastin
    Yi Pin Lin
    Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, New York, USA
    J Biol Chem 284:19380-91. 2009
    ..In this study, we report that Leptospira can be immobilized by human tropoelastin (HTE) or elastin from different tissues, including lung, skin, and blood vessels, and that Lig proteins can ..
  29. ncbi Development of a new in vitro model of elastic fiber assembly in human pigmented epithelial cells
    Hiroshi Wachi
    Department of Clinical Chemistry, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2 4 41 Ebara, Shinagawa ku, Tokyo 142 8501, Japan
    Clin Biochem 38:643-53. 2005
    We developed an in vitro model of elastic fiber assembly that provides a comparison of the efficiency of different tropoelastin molecules to organize into fibers.
  30. ncbi The cysteine residues in the carboxy terminal domain of tropoelastin form an intrachain disulfide bond that stabilizes a loop structure and positively charged pocket
    P L Brown
    Department of Cell Biology, Jewish Hospital, Washington University Medical Center, St Louis, MO 63110
    Biochem Biophys Res Commun 186:549-55. 1992
    Analysis of purified bovine tropoelastin with Ellman's reagent and [14C]iodoacetamide demonstrated that the only two cysteine residues in the molecule form an intrachain disulfide bond...
  31. ncbi Fibrillin-1 interactions with fibulins depend on the first hybrid domain and provide an adaptor function to tropoelastin
    Ehab El-Hallous
    Faculty of Medicine, Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec H3A 2B2, Canada
    J Biol Chem 282:8935-46. 2007
    ..Formation of ternary complexes between fibrillin-1, fibulins, and tropoelastin demonstrated that fibulin-2 and -5 but much less fibulin-4, are able to act as molecular adaptors between ..
  32. ncbi Autosomal dominant cutis laxa with severe lung disease: synthesis and matrix deposition of mutant tropoelastin
    Zsolt Urban
    Department of Anatomy, University of Hawaii, Honolulu, Hawaii, USA
    J Invest Dermatol 124:1193-9. 2005
    ..In addition to the normal 68 kDa tropoelastin (TE) protein, an abnormal, 120 kDa polypeptide was detected in the proband and her affected daughter in a CL ..
  33. ncbi Heparan sulfate regulates fibrillin-1 N- and C-terminal interactions
    Stuart A Cain
    Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, United Kingdom
    J Biol Chem 283:27017-27. 2008
    ..by exons 59-62 near the fibrillin-1 C terminus are novel conformation-dependent high affinity heparin and tropoelastin binding sites...
  34. ncbi Molecular basis of elastic fiber formation. Critical interactions and a tropoelastin-fibrillin-1 cross-link
    Matthew J Rock
    Wellcome Trust Centre for Cell-Matrix Research, School of Biological Sciences, University of Manchester, 2.205 Stopford Building, Oxford Road, Manchester M13 9PT, United Kingdom
    J Biol Chem 279:23748-58. 2004
    ..binding of two overlapping fibrillin-1 fragments (encoded by central exons 18-25 and 24-30) to tropoelastin, which, in microfibrils, map to an exposed "arms" feature adjacent to the beads...
  35. ncbi Fibroblast tropoelastin and alpha-smooth-muscle actin expression are repressed by particulate-activated macrophage-derived tumor necrosis factor-alpha in experimental silicosis
    T J Mariani
    Department of Internal Medicine, Washington University School of Medicine at Barnes Jewish Hospital, St Louis, Missouri, USA
    Am J Respir Cell Mol Biol 21:185-92. 1999
    ..Reinitiated tropoelastin expression was identified throughout the lung in fibroblasts expressing alpha-smooth-muscle actin, whereas ..
  36. ncbi Coacervation is promoted by molecular interactions between the PF2 segment of fibrillin-1 and the domain 4 region of tropoelastin
    Adam W Clarke
    School of Molecular and Microbial Biosciences G08, University of Sydney, Sydney, New South Wales, 2006, Australia
    Biochemistry 44:10271-81. 2005
    In forming elastic fibers, microfibrils act as the scaffold sites for depositing the elastin precursor tropoelastin. We examined key binding interactions that promote massive tropoelastin association through coacervation...
  37. ncbi Fibrillin-1 interactions with heparin. Implications for microfibril and elastic fiber assembly
    Stuart A Cain
    Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, United Kingdom
    J Biol Chem 280:30526-37. 2005
    ..cell attachment, but heparin and MAGP-1 competed for binding to the fibrillin-1 N terminus, and heparin and tropoelastin competed for binding to a central fibrillin-1 sequence...
  38. ncbi Microfibril-associated glycoprotein-1 and fibrillin-2 are associated with tropoelastin deposition in vitro
    Eichi Tsuruga
    Department of Oral Anatomy, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari Tobetsu, Hokkaido 061 0293, Japan
    Int J Biochem Cell Biol 37:120-9. 2005
    Elastic system fibers consist of microfibrils and tropoelastin. During development, microfibrils act as a template on which tropoelastin is deposited...
  39. ncbi Identification of tropoelastin as a ligand for the 65-kD FK506-binding protein, FKBP65, in the secretory pathway
    E C Davis
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Cell Biol 140:295-303. 1998
    The folding and trafficking of tropoelastin is thought to be mediated by intracellular chaperones, although the identity and role of any tropoelastin chaperone remain to be determined...
  40. ncbi Glycosaminoglycan-mediated coacervation of tropoelastin abolishes the critical concentration, accelerates coacervate formation, and facilitates spherule fusion: implications for tropoelastin microassembly
    Yidong Tu
    School of Molecular and Microbial Biosciences G08, University of Sydney, NSW 2006, Australia
    Biomacromolecules 9:1739-44. 2008
    Elastogenesis and elastin repair depend on the secretion of tropoelastin from the cell, yet cellular production is low in the many biological systems that have been studied...
  41. ncbi Elastase-released epidermal growth factor recruits epidermal growth factor receptor and extracellular signal-regulated kinases to down-regulate tropoelastin mRNA in lung fibroblasts
    Sandra J DiCamillo
    Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    J Biol Chem 277:18938-46. 2002
    ..with transient, peaking at 30 min, induction of c-Fos and sustained, observed at 24-48 h, decrease of tropoelastin mRNA levels in NE-challenged cells...
  42. ncbi Fibrillin-1 and fibrillin-2 show temporal and tissue-specific regulation of expression in developing elastic tissues
    M C Mariencheck
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Connect Tissue Res 31:87-97. 1995
    ..these possibilities, the expression of fibrillin-1 and fibrillin-2 was compared with expression of MAGP and tropoelastin in two elastogenic tissues that undergo different developmental programs...
  43. ncbi Induction of fibulin-5 gene is regulated by tropoelastin gene, and correlated with tropoelastin accumulation in vitro
    Eichi Tsuruga
    Department of Oral Anatomy, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari Tobetsu, Hokkaido 061 0293, Japan
    Int J Biochem Cell Biol 36:395-400. 2004
    ..examined the relationship between the gene expression of fibulin-5 and the gene expression and accumulation of tropoelastin by comparing elastin-producing cells (human gingival fibroblasts) with non-elastin-producing cells (human ..
  44. ncbi Gene expression of tropoelastin is enhanced in the aorta proximal to the coarctation in rabbits
    Chengpei Xu
    Department of Surgery, Stanford University, Division of Vascular Surgery, 300 Pasteur Drive, Suite H3600, Stanford, California, USA
    Exp Mol Pathol 72:115-23. 2002
    ..in the aortic wall in response to acute elevation of blood pressure, we studied the aortic gene expression of tropoelastin in a rabbit midthoracic aortic coarctation model...
  45. ncbi Expression of fibrillins and tropoelastin by human gingival and periodontal ligament fibroblasts in vitro
    Eichi Tsuruga
    Department of Oral Anatomy, School of Dentistry, Health Sciences University of Hokkaido, Ishikari Tobetsu, Japan
    J Periodontal Res 37:23-8. 2002
    ..the major components of microfibrils involved in elastogenesis, we investigated the synthesis of fibrillins and tropoelastin in the conditioned medium of HGF and HPLF...
  46. ncbi An elastin gene mutation producing abnormal tropoelastin and abnormal elastic fibres in a patient with autosomal dominant cutis laxa
    M Tassabehji
    University Department of Medical Genetics and Regional Genetics Service, St Mary s Hospital, Manchester M13 0JH, UK
    Hum Mol Genet 7:1021-8. 1998
    ..These findings suggest that the mutant tropoelastin protein is synthesized, secreted and incorporated into the elastic matrix, where it alters the architecture of ..
  47. ncbi Action of tropoelastin and synthetic elastin sequences on vascular tone and on free Ca2+ level in human vascular endothelial cells
    G Faury
    Laboratoire de Bioénergétique Fondamentale et Appliquée, Universite Joseph Fourier, Grenoble, France
    Circ Res 82:328-36. 1998
    ..we have compared the action of different concentrations (10(-13) to 10(-7) mol/L) of recombinant human tropoelastin, eight synthetic elastin peptides, and a control peptide (VPVGGA) on both rat aortic ring tension and [Ca2+]i ..
  48. ncbi Ultraviolet radiation increases tropoelastin mRNA expression in the epidermis of human skin in vivo
    J Y Seo
    Department of Dermatology, Seoul National University College of Medicine, Seoul National University Hospital, Korea
    J Invest Dermatol 116:915-9. 2001
    ..In this study, it was demonstrated that ultraviolet irradiation induced tropoelastin mRNA expression in the keratinocytes of human skin in vivo and also in cultured human keratinocytes by in situ ..
  49. ncbi Retrovirally mediated overexpression of glycosaminoglycan-deficient biglycan in arterial smooth muscle cells induces tropoelastin synthesis and elastic fiber formation in vitro and in neointimae after vascular injury
    Jin Yong Hwang
    Hope Heart Program, Benaroya Research Institute, Seattle, WA 98101, USA
    Am J Pathol 173:1919-28. 2008
    ..Cultured cells overexpressing LmBSN showed marked up-regulation of tropoelastin and fibulin-5 mRNAs, increased amounts of desmosine and insoluble elastin, and increased deposition of elastic ..
  50. ncbi Use of an intron polymorphism to localize the tropoelastin gene to mouse chromosome 5 in a region of linkage conservation with human chromosome 7
    K S Wydner
    Department of Biological Sciences, Rutgers University, Piscataway, New Jersey 08855 1059
    Genomics 23:125-31. 1994
    The complete coding sequence for mouse tropoelastin was obtained from overlapping reverse transcriptase polymerase chain reaction (PCR) amplimers...
  51. ncbi DANCE/fibulin-5 promotes elastic fiber formation in a tropoelastin isoform-dependent manner
    Risa Nonaka
    Department of Clinical Chemistry, Hoshi University School of Pharmacy and Pharmaceutical Sciences, Tokyo, Japan
    Clin Biochem 42:713-21. 2009
    ..of fibulin-5 in the elastic fiber formation, we studied the molecular interactions among elastin, fibrillin-1, and fibulin-5 in the extracellular space and the maturation of tropoelastin using retinal pigment epithelial cells (ARPE-19).
  52. ncbi Domains 16 and 17 of tropoelastin in elastic fibre formation
    Hiroshi Wachi
    Department of Clinical Chemistry, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2 4 41 Ebara, Shinagawa ku, Tokyo 142 8501, Japan
    Biochem J 402:63-70. 2007
    ..b>Tropoelastin lacking domains 16-17 (Delta16-17) was synthesized efficiently and secreted by transfected retinal pigment ..
  53. ncbi Heterogeneity of rat tropoelastin mRNA revealed by cDNA cloning
    R A Pierce
    Department of Surgery, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, New Brunswick 08903 0019
    Biochemistry 29:9677-83. 1990
    ..gt11 library constructed from poly(A+) RNA isolated from aortic tissue of neonatal rats was screened for rat tropoelastin cDNAs. The first screen, utilizing a human tropoelastin cDNA clone, provided rat tropoelastin cDNAs spanning 2...
  54. ncbi Analysis of dermal elastic fibers in the absence of fibulin-5 reveals potential roles for fibulin-5 in elastic fiber assembly
    Jiwon Choi
    Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada
    Matrix Biol 28:211-20. 2009
    ..lower levels of mature cross-linked elastin in the Fbln5(-/-) dermis, however, gene expression levels for tropoelastin and fibrillin-1, the major elastic fiber components, were unaffected...
  55. ncbi Tropoelastin binding to fibulins, nidogen-2 and other extracellular matrix proteins
    T Sasaki
    Max Planck Institut fur Biochemie, Am Klopferspitz 18a, D 82152, Martinsried, Germany
    FEBS Lett 460:280-4. 1999
    Elastic fibers in vessel walls and other tissues consist of cross-linked tropoelastin in association with several microfibrillar proteins...
  56. ncbi High copper levels and increased elastolysis in a patient with cutis marmorata teleangiectasia congenita
    Aleksander Hinek
    Division of Cardiovascular Research, The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada
    Am J Med Genet A 146:2520-7. 2008
    ..cultures (passage one) of dermal fibroblasts derived from this patient were capable of normal synthesis of tropoelastin, but their net deposition of mature elastic fibers was significantly diminished as compared with cultures of ..
  57. ncbi Dissection of human tropoelastin: supramolecular organization of polypeptide sequences coded by particular exons
    Antonietta Pepe
    Department of Chemistry, Universita della Basilicata, Via N Sauro 85, 85100 Potenza, Italy
    Matrix Biol 24:96-109. 2005
    Polypeptide sequences encoded by some exons of the human tropoelastin gene (EDP, elastin-derived peptide) have been analysed for their ability to coacervate and to self-assembly...
  58. ncbi Quantitative analysis for soluble elastin in circulation and cell culture fluids using monoclonal antibody-based sandwich immunoassay
    Tadashi Shinohara
    Internal Medicine I, National Defense Medical College, Saitama, Japan
    J Immunoassay Immunochem 26:189-202. 2005
    ..581, p < 0.0001). In addition, we could also determine the concentration of tropoelastin secreted from cultured human dermal fibroblasts accurately by this ELISA...
  59. ncbi VE-statin/egfl7 regulates vascular elastogenesis by interacting with lysyl oxidases
    Etienne Lelievre
    CNRS, UMR 8161, Institut de Biologie de Lille, Equipe labellisée La Ligue 2005, Universite de Lille I, Universite de Lille II, Institut Pasteur de Lille, Lille, France
    EMBO J 27:1658-70. 2008
    ..the presence of VE-statin/egfl7 were unable to deposit elastic fibres due to a deficient conversion of soluble tropoelastin into insoluble mature elastin...
  60. ncbi Cellular interactions with elastin
    Ursula R Rodgers
    School of Molecular and Microbial Biosciences, University of Sydney, NSW, Australia
    Pathol Biol (Paris) 53:390-8. 2005
    Elastin is a key structural component of the extracellular matrix. Tropoelastin is the soluble precursor of elastin...
  61. ncbi Mapping of macrophage elastase cleavage sites in insoluble human skin elastin
    Samuel Taddese
    Institute of Pharmacy, Martin Luther University Halle Wittenberg, Halle Saale, Germany
    Matrix Biol 27:420-8. 2008
    ..The amino acid preferences of the different subsites on the catalytic domain of MMP-12 were analyzed...
  62. ncbi Structural determinants of cross-linking and hydrophobic domains for self-assembly of elastin-like polypeptides
    Ming Miao
    Research Institute, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada
    Biochemistry 44:14367-75. 2005
    ..To achieve the required structural durability of the elastic matrix, the elastin monomer, tropoelastin, undergoes ordered assembly into a covalently cross-linked, fibrillar polymeric structure...
  63. ncbi Locally generated VGVAPG and VAPG elastin-derived peptides amplify melanoma invasion via the galectin-3 receptor
    Peter Pocza
    Department of Genetics, Cell, and Immunbiology, Semmelweis University, Nagyvarad ter 4, Budapest H 1089, Hungary
    Int J Cancer 122:1972-80. 2008
    ..Elastin, tropoelastin and VGVAPG peptide were demonstrated at the invasion site of melanoma using histochemistry and ..
  64. ncbi Sequences and domain structures of mammalian, avian, amphibian and teleost tropoelastins: Clues to the evolutionary history of elastins
    Martin I S Chung
    Cardiovascular Research Program, Research Institute, Hospital for Sick Children, Toronto, ON, Canada M5G1X8
    Matrix Biol 25:492-504. 2006
    b>Tropoelastin is the monomeric form of elastin, a polymeric extracellular matrix protein responsible for properties of extensibility and elastic recoil in connective tissues of most vertebrates...
  65. ncbi Aldosterone induces elastin production in cardiac fibroblasts through activation of insulin-like growth factor-I receptors in a mineralocorticoid receptor-independent manner
    Severa Bunda
    Cardiovascular Research Program, University of Toronto, Ontario, Canada
    Am J Pathol 171:809-19. 2007
    ..Most importantly, we demonstrated that aldosterone also increases elastin mRNA levels, tropoelastin synthesis, and elastic fiber deposition in a dose-dependent manner...
  66. ncbi Localizing alpha-helices in human tropoelastin: assembly of the elastin "puzzle"
    Antonio Mario Tamburro
    Department of Chemistry, Universita degli Studi della Basilicata, Via N Sauro, 85, 85100 Potenza, Italy
    Biochemistry 45:9518-30. 2006
    Polyalanine cross-linking domains encoded by exons 6, 15, 17, 19, 21, 23, 25, 27, 29, 31 of human tropoelastin were synthesized, and their conformations were studied in different solutions and at different temperatures by CD and (1)H NMR...
  67. ncbi Significant decrease in tropoelastin gene expression in fibroblasts from a Japanese Costello syndrome patient with impaired elastogenesis and enhanced proliferation
    Yutaka Tatano
    Department of Medicinal Biotechnology, Institute for Medicinal Resources, Graduate School of Pharmaceutical Sciences, The University of Tokushima, Tokushima 770 8505
    J Biochem 140:193-200. 2006
    ..In this study we demonstrated a significant decrease in the expression of tropoelastin mRNA in fibroblasts derived from a Japanese Costello syndrome patient with impaired elastogenesis and enhanced ..
  68. ncbi Disordered pulmonary myofibroblast distribution and elastin expression in preterm infants with Ureaplasma urealyticum pneumonitis
    Rose Viscardi
    Department of Pediatrics, University of Maryland School of Medicine, 22 South Greene Street, Baltimore, 21201, USA
    Pediatr Dev Pathol 9:143-51. 2006
    ..and transforming growth factor beta1 (TGFbeta1), Hart's elastin staining, and in situ hybridization for tropoelastin (TE) expression...
  69. ncbi Ellagic and tannic acids protect newly synthesized elastic fibers from premature enzymatic degradation in dermal fibroblast cultures
    Felipe Jimenez
    Research Department, Human Matrix Sciences, LLC, Visalia, California, USA
    J Invest Dermatol 126:1272-80. 2006
    ..Results of a pulse and chase experiment showed that pretreatment with both polyphenols enhanced biostability of tropoelastin and newly deposited elastin...
  70. ncbi Quantitative and comparative studies of the vocal fold extracellular matrix. I: Elastic fibers and hyaluronic acid
    Mariah S Hahn
    Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Boston, USA
    Ann Otol Rhinol Laryngol 115:156-64. 2006
    ..The distributions of fibrillin-1, a primary microfibrillar component of elastic fibers, and of tropoelastin, an indicator of elastin synthesis, were immunohistochemically analyzed. RESULTS: Elastin and HA constituted 8...
  71. ncbi Regulation of elastin promoter by lysyl oxidase and growth factors: cross control of lysyl oxidase on TGF-beta1 effects
    Roberta Oleggini
    Laboratorio di Nefrologia, Istituto G Gaslini, Genova, Italy
    Matrix Biol 26:494-505. 2007
    ..Many of those included SMAD transcription factors. Two more restricted regions binding AP1 and SMAD were identified as mediators of LOX effects and of LOX and TGF-beta1 cross-inhibition...
  72. ncbi Investigating the amyloidogenic nanostructured sequences of elastin: sequence encoded by exon 28 of human tropoelastin gene
    Brigida Bochicchio
    Department of Chemistry, Universita della Basilicata, Via N Sauro 85, Potenza, Italy
    Biomacromolecules 8:3478-86. 2007
    In this paper we demonstrate that the sequence encoded by exon 28 (EX28) of human tropoelastin gene is able to give amyloid-like fibrils...
  73. ncbi Elastic fiber formation: a dynamic view of extracellular matrix assembly using timer reporters
    Beth A Kozel
    Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri 63110, USA
    J Cell Physiol 207:87-96. 2006
    ..the dynamics of elastic fiber assembly, mammalian cells were transfected with a cDNA construct encoding bovine tropoelastin in frame with the Timer reporter...
  74. ncbi Granulocyte macrophage colony-stimulating factor deficiency affects vascular elastin production and integrity of elastic lamellae
    Gabriele Weissen-Plenz
    Leibniz Institute for Arteriosclerosis Research, Munster, Germany
    J Vasc Res 45:103-10. 2008
    ..The present study elaborates the effect of GM-CSF deficiency on the vascular elastin system...
  75. ncbi Elastic fiber macro-assembly is a hierarchical, cell motion-mediated process
    Andras Czirok
    Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA
    J Cell Physiol 207:97-106. 2006
    ..It was recently demonstrated that in vitro, cells first secrete tropoelastin into a punctate pattern of globules...
  76. ncbi Phenotypic alteration of vascular smooth muscle cells precedes elastolysis in a mouse model of Marfan syndrome
    T E Bunton
    Department of Comparative Medicine, Institute for Genetic Medicine, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    Circ Res 88:37-43. 2001
    ..This refined understanding of the pathogenesis of vascular disease in Marfan syndrome will facilitate the development of therapeutic strategies...
  77. ncbi Elastin induces myofibrillogenesis via a specific domain, VGVAPG
    Satyajit K Karnik
    Program in Human Molecular Biology and Genetics and Department of Oncological Sciences, University of Utah, 15 North 2030 East Rm 4450, Salt Lake City, UT 84112 5330, USA
    Matrix Biol 22:409-25. 2003
    ..Previously, we showed that the Elastin gene product, tropoelastin, activates myofibrillar organization of VSMCs...
  78. ncbi Multiple chick tropoelastin mRNAs
    V J Baule
    Department of Biology, Syracuse University, New York 13244 1220
    Biochem Biophys Res Commun 154:1054-60. 1988
    Several overlapping chick tropoelastin cDNAs were isolated from a lambda gt11 cDNA library constructed from whole 10 day chick embryo total RNA. Comparison of the nucleotide sequence of the 2...
  79. ncbi Rat tropoelastin is synthesized from a 3.5-kilobase mRNA
    S B Deak
    Department of Surgery, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, New Brunswick 08903
    J Biol Chem 263:13504-7. 1988
    ..was constructed from poly(A+) RNA isolated from aortic tissue of neonatal rats and screened with a human tropoelastin cDNA clone...
  80. ncbi Elastin: relation of protein and gene structure to disease
    J Rosenbloom
    Lab Invest 51:605-23. 1984
    ..studies and in vitro translation of elastin mRNA have demonstrated that a 72,000-dalton polypeptide, designated tropoelastin, is the initial translation product...
  81. ncbi Nature and the multiple functions of the 67-kD elastin-/laminin binding protein
    A Hinek
    Division of Cardiovascular Research, Hospital for Sick Children, Toronto, Canada
    Cell Adhes Commun 2:185-93. 1994
    ..I will emphasize the role of the 67-kD protein in divergent cellular processes, such as cell-matrix attachment, matrix assembly, cellular chemotaxis, and active migration through the vascular walls...
  82. ncbi Domains in tropoelastin that mediate elastin deposition in vitro and in vivo
    Beth A Kozel
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 278:18491-8. 2003
    ..To identify domains in the tropoelastin molecule important for the assembly process, we utilized an in vitro assembly model to map sequences within ..
  83. ncbi Effect of intratracheal adenoviral vector administration on lung development in newborn rats
    Paul Waszak
    INSERM U492, , , France
    Hum Gene Ther 13:1873-85. 2002
    ..02), as well as a 23% decrease in absolute parenchyma elastic fiber length (p = 0.02). Furthermore, lung tropoelastin mRNA content decreased by 25% (p < 0.02)...
  84. ncbi Functional domains on elastin and microfibril-associated glycoprotein involved in elastic fibre assembly
    P Brown-Augsburger
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, MO 63110, USA
    Biochem J 318:149-55. 1996
    Studies in vitro suggest that the C-terminus of tropoelastin mediates elastin polymerization through an interaction with microfibril-associated proteins...
  85. ncbi Elastic fibers: building bridges between cells and their matrix
    Kim S Midwood
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544 1014, USA
    Curr Biol 12:R279-81. 2002
    ..Extracellular elastic fibers confer resilience and flexibility to tissues. Recent studies have identified a protein, fibulin-5, that connects these fibers to cells and regulates their assembly and organization...
  86. ncbi Nitric oxide stimulates elastin expression in chick aortic smooth muscle cells
    H Sugitani
    Department of Clinical Chemistry, Hoshi College of Pharmacy, Tokyo, Japan
    Biol Pharm Bull 24:461-4. 2001
    ..S-Nitrosoglutathione (GSNO), a NO donor, stimulated tropoelastin synthesis in cultured SMCs during both the quiescent and proliferating phases...
  87. ncbi [Effect of elastin peptides on the production of matrix metalloproteinase 2 by human skin fibroblasts in culture]
    E Huet
    Signalisation Cellulaire et Matrice Extracellulaire, CNRS FRE 2260, , , , Reims, France
    J Soc Biol 195:165-72. 2001
    Soluble elastin-derived peptides from alkaline or elastase hydrolysis of insoluble elastin, as well as tropoelastin, increase matrix metalloproteinase-2 (MMP-2) production by human skin fibroblasts in culture as determined by gelatin ..
  88. ncbi Impaired elastic-fiber assembly by fibroblasts from patients with either Morquio B disease or infantile GM1-gangliosidosis is linked to deficiency in the 67-kD spliced variant of beta-galactosidase
    A Hinek
    Division of Cardiovascular Research, The Hospital for Sick Children, Toronto, Ontario, Canada
    Am J Hum Genet 67:23-36. 2000
    We have previously shown that intracellular trafficking and extracellular assembly of tropoelastin into elastic fibers is facilitated by the 67-kD elastin-binding protein identical to an enzymatically inactive, alternatively spliced ..
  89. ncbi ECM gene expression correlates with in vitro tissue growth and development in fibrin gel remodeled by neonatal smooth muscle cells
    J J Ross
    Departments of Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455, USA
    Matrix Biol 22:477-90. 2003
    ..Following fibrin gel contraction during week 1, peak rates of SMC proliferation, collagen production and tropoelastin production occurred between weeks 1-4...
  90. ncbi FGF-18 is upregulated in the postnatal rat lung and enhances elastogenesis in myofibroblasts
    Bernadette Chailley-Heu
    Physiopathologie et Thérapeutique Respiratoires, Institut National de la Santè et de la Recherche Mèdicale U492, Faculte de Medecine, 94010 Creteil Cedex, France
    Am J Physiol Lung Cell Mol Physiol 288:L43-51. 2005
    ..5-fold), a characteristic marker of myofibroblasts, of tropoelastin (6.5-fold), of lysyl oxidase (2-fold), and of fibulins 1 and 5 (8- and 2...
  91. ncbi Deposition of tropoelastin into the extracellular matrix requires a competent elastic fiber scaffold but not live cells
    Beth A Kozel
    Department of Cell Biology and Physiology, Washington University School of Medicine, Campus Box 8228, 660 South Euclid Ave, St Louis, MO 63110, USA
    Matrix Biol 23:23-34. 2004
    ..of elastic fiber assembly were investigated using an in vitro assembly model in which purified recombinant tropoelastin (rbTE) was added to cultures of live or dead cells...
  92. ncbi B-Myb represses elastin gene expression in aortic smooth muscle cells
    Claudia S Hofmann
    Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    J Biol Chem 280:7694-701. 2005
    ..Here we sought to determine whether elastin is similarly repressed by B-Myb. Levels of tropoelastin mRNA and protein were lower in aortas and isolated SMCs of adult transgenic mice expressing the human B-myb ..
  93. ncbi Pulmonary elastin synthesis and deposition in developing and mature sheep: effects of intrauterine growth restriction
    Megan L Cock
    Fetal and Neonatal Research Group, Department of Physiology, Monash University, Clayton, Victoria, Australia
    Exp Lung Res 30:405-18. 2004
    ..Pulmonary tropoelastin expression and elastin content were examined at 128 days (5 days UPE) and 140 days (20 days UPE) of the 147- ..
  94. ncbi Elastogenesis in human arterial disease: a role for macrophages in disordered elastin synthesis
    Alexandra Krettek
    Leducq Center for Cardiovascular Research, Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 221 Longwood Avenue, Eugene Braunwald Research Center 307, Boston, Mass 02115, USA
    Arterioscler Thromb Vasc Biol 23:582-7. 2003
    ..METHODS AND RESULTS: Human AAA and atheroma have 4- to 6-fold more tropoelastin protein than nondiseased arteries...
  95. ncbi Role of tropoelastin fragmentation in elastogenesis in rat smooth muscle cells
    C Franzblau
    Department of Biochemistry, Boston University School of Medicine, Massachusetts 02118
    J Biol Chem 264:15115-9. 1989
    ..smooth muscle cell cultures produce two major soluble elastin molecules termed protropoelastin (77 kDa) and tropoelastin (71 kDa)...
  96. ncbi Spectroscopic evidence revealing polyproline II structure in hydrophobic, putatively elastomeric sequences encoded by specific exons of human tropoelastin
    Brigida Bochicchio
    , LSSBM EA-3305, , , UFR Sciences Exactes et Naturelles, BP 1039, 51687 Reims Cedex 2, France
    Biopolymers 73:484-93. 2004
    ..Given the cassette like organization of the human tropoelastin gene, giving rise to alternating exons encoding for crosslink domains and elastomeric domains, it is tempting ..
  97. ncbi Effect of L-fucose and fucose-rich polysaccharides on elastin biosynthesis, in vivo and in vitro
    L Robert
    Laboratoire Universitaire de Recherche en Ophtalmologie, Faculté de Médecine Broussais Hôtel Dieu, Universite Pierre et Marie Curie, Paris, France
    Biomed Pharmacother 58:123-8. 2004
    ..in explant cultures, the addition of L-fucose or of FROP-3 increased the biosynthesis of immunoprecipitable tropoelastin by about 40%...
  98. ncbi Characteristic properties of proteins from pre-ecdysial cuticle of larvae and pupae of the mealworm Tenebrio molitor
    S O Andersen
    Biochemical Department, August Krogh Institute, University of Copenhagen, Universitetsparken 13, DK 2100 Copenhagen, Denmark
    Insect Biochem Mol Biol 32:1077-87. 2002
    ..The behaviour of the cuticular proteins in solution resembles that of another hydrophobic protein, tropoelastin, and it seems reasonable to suggest that similar interactions govern the folding and aggregation of the peptide ..
  99. ncbi Developmental regulation and coordinate reexpression of FKBP65 with extracellular matrix proteins after lung injury suggest a specialized function for this endoplasmic reticulum immunophilin
    Charles E Patterson
    Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas 75390 9039, USA
    Cell Stress Chaperones 10:285-95. 2005
    ..we have shown that FKBP65 is developmentally regulated and associates with the extracellular matrix protein, tropoelastin, during its maturation and transport through the ER...
  100. ncbi Synthetic elastin hydrogels that are coblended with heparin display substantial swelling, increased porosity, and improved cell penetration
    Yidong Tu
    School of Molecular Bioscience, University of Sydney, NSW, Australia
    J Biomed Mater Res A 95:1215-22. 2010
    ..To overcome this problem, glycosaminoglycans were coblended with tropoelastin during the formation of synthetic elastin hydrogels...
  101. ncbi Utility of hyaluronan oligomers and transforming growth factor-beta1 factors for elastic matrix regeneration by aneurysmal rat aortic smooth muscle cells
    Chandrasekhar R Kothapalli
    Department of Bioengineering, Clemson University, Clemson, South Carolina, USA
    Tissue Eng Part A 15:3247-60. 2009
    ..and 9 elastase activity and calcific deposits, while synthesizing significantly less collagen, tropoelastin, and matrix elastin proteins over a 3-week culture period, relative to healthy SMCs...

Research Grants74

  1. INTRACELLULAR TRAFFICKING OF TROPOELASTIN
    Elaine Davis; Fiscal Year: 2001
    Elastic fiber assembly is a complicated process that involves the organization of tropoelastin monomers on a microfibril scaffold and the subsequent crosslinking of these monomers into an insoluble elastin matrix...
  2. Cues for cell-mediated regeneration of elastin matrix to stabilize aortic aneurys
    Anand Ramamurthi; Fiscal Year: 2010
    ..wherein RASMCs (healthy and aneurysmal) will be provided cues to both synthesize, and assemble and crosslink tropoelastin precursors...
  3. Cues for cell-mediated regeneration of elastin matrix to stabilize aortic aneurys
    Anand Ramamurthi; Fiscal Year: 2009
    ..wherein RASMCs (healthy and aneurysmal) will be provided cues to both synthesize, and assemble and crosslink tropoelastin precursors...
  4. Cues for cell-mediated regeneration of elastin matrix to stabilize aortic aneurys
    Anand Ramamurthi; Fiscal Year: 2009
    ..wherein RASMCs (healthy and aneurysmal) will be provided cues to both synthesize, and assemble and crosslink tropoelastin precursors...
  5. INTRACELLULAR TRAFFICKING OF TROPOELASTIN
    Frederick Grinnell; Fiscal Year: 2002
    Elastic fiber assembly is a complicated process that involves the organization of tropoelastin monomers on a microfibril scaffold and the subsequent crosslinking of these monomers into an insoluble elastin matrix...
  6. FIBULIN-5 & WALL STRESSES IN VASCULAR REMODELING: THEORY AND EX VIVO EXPERIMENTS
    RUDOLPH GLEASON; Fiscal Year: 2007
    ..Fibulin-5 is an ECM protein that binds tropoelastin and fibrillins with ava3, ava5, and a9a1 integrins(60) to bridge elastic fibers with cells...
  7. Disorganization of Elastin Matrix Mediates Erectile Dysfunction
    KANCHAN A CHITALEY; Fiscal Year: 2010
    ..By maturity, assembly of elastic fibers is complete, and synthesis of new tropoelastin, the soluble precursor, has declined...
  8. Elastin Gene Mutations: Mechanisms Causing SVAS and ADCL
    Zsolt Urban; Fiscal Year: 2005
    ..In ADCL patients, in contrast, we have identified mutations that result in the expression of mutant tropoelastin. Based on these results, we hypotesize that different classes of elastin gene mutations result in SVAS and ADCL ..
  9. EXTRACELLULAR MATRIX REPAIR IN PULMONARY EMPHYSEMA
    Steven Shapiro; Fiscal Year: 2002
    ..Elastic fiber assembly is complex requiring tropoelastin monomers to be aligned on a scaffold of potentially several microfibril proteins where they are then cross-..
  10. Regulation of Vascular Wall Extracellular Matrix
    William Parks; Fiscal Year: 2006
    ..Elastin is a covalently crosslinked polymer of tropoelastin, the secreted precursor protein, which are assembled into fibers on a microfibrillar scaffold...
  11. Bioengineering & Biologic Studies of Aneurysm Weakening
    David Vorp; Fiscal Year: 2009
    ..to either degradation or maintenance of wall integrity, such as matrix metalloproteinases and ECM precursors tropoelastin and procollagen. The results of this study could have an immediate impact on the clinical management of AAA...
  12. Functional Repair of Elastin with Recomb. Tropoelastin
    Phillip Stone; Fiscal Year: 2004
    ..this goal our objectives are to regenerate elastin in tissue culture systems by crosslinking recombinant tropoelastin (rTE) into damaged elastin and to characterize the effectiveness of this regeneration by assessing the ..
  13. MOLECULAR BIOLOGY OF THE MARFAN SYNDROME
    Harry Dietz; Fiscal Year: 2004
    ..The prevailing view has been that microfibrils regulate the deposition of tropoelastin in late fetal life...
  14. MECHANISMS FOR CHRONIC UVR-INDUCED DAMAGE TO SKIN
    IRENE KOCHEVAR; Fiscal Year: 2003
    ..The processes involved in stimulating synthesis of tropoelastin by fibroblasts in response to UVR are only inadequately understood...
  15. POSTTRANSCRIPTIONAL REGULATION OF TROPOELASTIN EXPRESSIO
    William Parks; Fiscal Year: 2001
    ..Elastin, the principal component of elastic fibers, is the product of crosslinked tropoelastin monomers...