tropoelastin

Summary

Summary: A salt-soluble precursor of elastin. Lysyl oxidase is instrumental in converting it to elastin in connective tissue.

Top Publications

  1. ncbi The immobilization of recombinant human tropoelastin on metals using a plasma-activated coating to improve the biocompatibility of coronary stents
    Anna Waterhouse
    School of Molecular Bioscience, University of Sydney, NSW 2006, Australia
    Biomaterials 31:8332-40. 2010
  2. pmc New insights into the pathogenesis of autosomal-dominant cutis laxa with report of five ELN mutations
    Bert Callewaert
    Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium
    Hum Mutat 32:445-55. 2011
  3. ncbi Expression of fibrillins and tropoelastin by human gingival and periodontal ligament fibroblasts in vitro
    Eichi Tsuruga
    Department of Oral Anatomy, School of Dentistry, Health Sciences University of Hokkaido, Ishikari Tobetsu, Japan
    J Periodontal Res 37:23-8. 2002
  4. pmc Retrovirally mediated overexpression of glycosaminoglycan-deficient biglycan in arterial smooth muscle cells induces tropoelastin synthesis and elastic fiber formation in vitro and in neointimae after vascular injury
    Jin Yong Hwang
    Hope Heart Program, Benaroya Research Institute, Seattle, WA 98101, USA
    Am J Pathol 173:1919-28. 2008
  5. ncbi Kinetics and morphology of self-assembly of an elastin-like polypeptide based on the alternating domain arrangement of human tropoelastin
    Judith T Cirulis
    Molecular Structure and Function Program, Research Institute, The Hospital for Sick Children, and Department of Biochemistry, University of Toronto, 555 University Avenue, Toronto, Ontario, Canada M5G1X8
    Biochemistry 49:5726-33. 2010
  6. ncbi An elastin gene mutation producing abnormal tropoelastin and abnormal elastic fibres in a patient with autosomal dominant cutis laxa
    M Tassabehji
    University Department of Medical Genetics and Regional Genetics Service, St Mary s Hospital, Manchester M13 0JH, UK
    Hum Mol Genet 7:1021-8. 1998
  7. pmc Fibulin-4 regulates expression of the tropoelastin gene and consequent elastic-fibre formation by human fibroblasts
    Qiuyun Chen
    Department of Molecular Cardiology, Lerner Research Institute, Cleveland Clinic, OH 44195, USA
    Biochem J 423:79-89. 2009
  8. pmc Molecular and supramolecular structural studies on human tropoelastin sequences
    Angela Ostuni
    Department of Chemistry, University of Basilicata, Potenza, Italy
    Biophys J 93:3640-51. 2007
  9. ncbi Domains 17-27 of tropoelastin contain key regions of contact for coacervation and contain an unusual turn-containing crosslinking domain
    L B Dyksterhuis
    School of Molecular and Microbial Biosciences, University of Sydney, NSW, Australia
    Matrix Biol 26:125-35. 2007
  10. pmc Cell adhesion to tropoelastin is mediated via the C-terminal GRKRK motif and integrin alphaVbeta3
    Daniel V Bax
    Applied and Plasma Physics, School of Physics, University of Sydney, Sydney, New South Wales 2006, Australia
    J Biol Chem 284:28616-23. 2009

Research Grants

Detail Information

Publications214 found, 100 shown here

  1. ncbi The immobilization of recombinant human tropoelastin on metals using a plasma-activated coating to improve the biocompatibility of coronary stents
    Anna Waterhouse
    School of Molecular Bioscience, University of Sydney, NSW 2006, Australia
    Biomaterials 31:8332-40. 2010
    ..We previously demonstrated a plasma-activated coating (PAC) that covalently bound recombinant human tropoelastin (TE), a major regulator of vascular cells in vivo, to enhance endothelial cell interactions...
  2. pmc New insights into the pathogenesis of autosomal-dominant cutis laxa with report of five ELN mutations
    Bert Callewaert
    Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium
    Hum Mutat 32:445-55. 2011
    ..Mutant protein expression in fibroblast cultures impaired deposition of tropoelastin onto microfibril-containing fibers, and enhanced tropoelastin coacervation and globule formation leading to ..
  3. ncbi Expression of fibrillins and tropoelastin by human gingival and periodontal ligament fibroblasts in vitro
    Eichi Tsuruga
    Department of Oral Anatomy, School of Dentistry, Health Sciences University of Hokkaido, Ishikari Tobetsu, Japan
    J Periodontal Res 37:23-8. 2002
    ..the major components of microfibrils involved in elastogenesis, we investigated the synthesis of fibrillins and tropoelastin in the conditioned medium of HGF and HPLF...
  4. pmc Retrovirally mediated overexpression of glycosaminoglycan-deficient biglycan in arterial smooth muscle cells induces tropoelastin synthesis and elastic fiber formation in vitro and in neointimae after vascular injury
    Jin Yong Hwang
    Hope Heart Program, Benaroya Research Institute, Seattle, WA 98101, USA
    Am J Pathol 173:1919-28. 2008
    ..Cultured cells overexpressing LmBSN showed marked up-regulation of tropoelastin and fibulin-5 mRNAs, increased amounts of desmosine and insoluble elastin, and increased deposition of elastic ..
  5. ncbi Kinetics and morphology of self-assembly of an elastin-like polypeptide based on the alternating domain arrangement of human tropoelastin
    Judith T Cirulis
    Molecular Structure and Function Program, Research Institute, The Hospital for Sick Children, and Department of Biochemistry, University of Toronto, 555 University Avenue, Toronto, Ontario, Canada M5G1X8
    Biochemistry 49:5726-33. 2010
    ..Both tropoelastin (TE), the full-length monomeric form of elastin, and elastin-like polypeptides (ELPs), based on sequences and ..
  6. ncbi An elastin gene mutation producing abnormal tropoelastin and abnormal elastic fibres in a patient with autosomal dominant cutis laxa
    M Tassabehji
    University Department of Medical Genetics and Regional Genetics Service, St Mary s Hospital, Manchester M13 0JH, UK
    Hum Mol Genet 7:1021-8. 1998
    ..These findings suggest that the mutant tropoelastin protein is synthesized, secreted and incorporated into the elastic matrix, where it alters the architecture of ..
  7. pmc Fibulin-4 regulates expression of the tropoelastin gene and consequent elastic-fibre formation by human fibroblasts
    Qiuyun Chen
    Department of Molecular Cardiology, Lerner Research Institute, Cleveland Clinic, OH 44195, USA
    Biochem J 423:79-89. 2009
    ..analysis showed that knockdown of fibulin-4 expression was accompanied by reduced expression of tropoelastin mRNA. Further analysis showed that this decrease was caused by transcriptional down-regulation of tropoelastin...
  8. pmc Molecular and supramolecular structural studies on human tropoelastin sequences
    Angela Ostuni
    Department of Chemistry, University of Basilicata, Potenza, Italy
    Biophys J 93:3640-51. 2007
    ..Previously, polypeptide sequences encoded by different exons of the human tropoelastin gene have been analyzed for their ability to coacervate and to self-assemble...
  9. ncbi Domains 17-27 of tropoelastin contain key regions of contact for coacervation and contain an unusual turn-containing crosslinking domain
    L B Dyksterhuis
    School of Molecular and Microbial Biosciences, University of Sydney, NSW, Australia
    Matrix Biol 26:125-35. 2007
    The central region of tropoelastin including domains 19-25 of human tropoelastin forms a hot-spot for contacts during the inter-molecular association of tropoelastin by coacervation [Wise, S.G., Mithieux, S.M., Raftery, M.J. and Weiss, A...
  10. pmc Cell adhesion to tropoelastin is mediated via the C-terminal GRKRK motif and integrin alphaVbeta3
    Daniel V Bax
    Applied and Plasma Physics, School of Physics, University of Sydney, Sydney, New South Wales 2006, Australia
    J Biol Chem 284:28616-23. 2009
    Elastin fibers are predominantly composed of the secreted monomer tropoelastin. This protein assembly confers elasticity to all vertebrate elastic tissues including arteries, lung, skin, vocal folds, and elastic cartilage...
  11. ncbi Thermodynamic and hydrodynamic properties of human tropoelastin. Analytical ultracentrifuge and pulsed field-gradient spin-echo NMR studies
    P Toonkool
    Department of Biochemistry and the Faculty of Pharmacy, University of Sydney, New South Wales 2006, Australia
    J Biol Chem 276:28042-50. 2001
    b>Tropoelastin is the soluble precursor of elastin that bestows tissue elasticity in vertebrates. Tropoelastin is soluble at 20 degrees C in phosphate-buffered saline, pH 7...
  12. ncbi Flexibility in the solution structure of human tropoelastin
    Lisa D Muiznieks
    School of Molecular and Microbial Biosciences, The University of Sydney, Sydney, Australia 2006
    Biochemistry 46:8196-205. 2007
    We investigated the flexibility of full-length tropoelastin in solution by using far- and near-ultraviolet circular dichroism (UV CD) and fluorescence spectroscopy to probe for structural flexibility and residue mobility within secondary ..
  13. pmc Repeated domains of leptospira immunoglobulin-like proteins interact with elastin and tropoelastin
    Yi Pin Lin
    Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, New York, USA
    J Biol Chem 284:19380-91. 2009
    ..In this study, we report that Leptospira can be immobilized by human tropoelastin (HTE) or elastin from different tissues, including lung, skin, and blood vessels, and that Lig proteins can ..
  14. pmc Domains 16 and 17 of tropoelastin in elastic fibre formation
    Hiroshi Wachi
    Department of Clinical Chemistry, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2 4 41 Ebara, Shinagawa ku, Tokyo 142 8501, Japan
    Biochem J 402:63-70. 2007
    ..b>Tropoelastin lacking domains 16-17 (Delta16-17) was synthesized efficiently and secreted by transfected retinal pigment ..
  15. ncbi The N-terminal A domain of Staphylococcus aureus fibronectin-binding protein A binds to tropoelastin
    Fiona M Keane
    Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College, Dublin 2, Ireland
    Biochemistry 46:7226-32. 2007
    ..Here we show that region A of FnBPA (rAFnBPA) binds to recombinant human tropoelastin. Binding occurs to three separate truncates of tropoelastin, encompassing domains 2-18, 17-27, and 27-36, ..
  16. ncbi Spectroscopic evidence revealing polyproline II structure in hydrophobic, putatively elastomeric sequences encoded by specific exons of human tropoelastin
    Brigida Bochicchio
    Laboratoire de Spectroscopies et Structures BioMoléculaires, LSSBM EA 3305, Universite de Reims Champagne Ardenne, IFR 53 Biomolecules, UFR Sciences Exactes et Naturelles, BP 1039, 51687 Reims Cedex 2, France
    Biopolymers 73:484-93. 2004
    ..Given the cassette like organization of the human tropoelastin gene, giving rise to alternating exons encoding for crosslink domains and elastomeric domains, it is tempting ..
  17. ncbi Heat modulation of tropoelastin, fibrillin-1, and matrix metalloproteinase-12 in human skin in vivo
    Zhou Chen
    Department of Dermatology, Seoul National University College of Medicine, Laboratory of Cutaneous Aging Research, Clinical Research Institute, Seoul National University Hospital, Institute of Dermatological Science, Seoul National University, Seoul, Korea
    J Invest Dermatol 124:70-8. 2005
    ..In this study, we investigated the effects of heat on the expression of tropoelastin and fibrillin-1, two main components of elastic fibers, and on matrix metalloproteinase (MMP)-12, the most ..
  18. ncbi Tropoelastin
    Steven G Wise
    School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia
    Int J Biochem Cell Biol 41:494-7. 2009
    b>Tropoelastin is a 60-72 kDa alternatively spliced extracellular matrix protein and a key component of elastic fibres. It is found in all vertebrates except for cyclostomes...
  19. ncbi Domains in tropoelastin that mediate elastin deposition in vitro and in vivo
    Beth A Kozel
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 278:18491-8. 2003
    ..To identify domains in the tropoelastin molecule important for the assembly process, we utilized an in vitro assembly model to map sequences within ..
  20. ncbi Gene expression and accumulation of fibrillin-1, fibrillin-2, and tropoelastin in cultured periodontal fibroblasts
    E Tsuruga
    Department of Oral Anatomy, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari Tobetsu, Hokkaido 061 0293, Japan
    J Dent Res 81:771-5. 2002
    ..to determine the gene expression of fibrillin-1 and fibrillin-2 (the major components of microfibrils) and of tropoelastin. In addition, we assessed the degree of accumulation of these proteins in the extracellular matrix...
  21. ncbi Structural changes and facilitated association of tropoelastin
    Lisa D Muiznieks
    School of Molecular and Microbial Biosciences G08, University of Sydney, Sydney, New South Wales 2006, Australia
    Arch Biochem Biophys 410:317-23. 2003
    Circular dichroism studies of tropoelastin secondary structure show 4+/-1% alpha-helix in aqueous solutions...
  22. ncbi Development of a new in vitro model of elastic fiber assembly in human pigmented epithelial cells
    Hiroshi Wachi
    Department of Clinical Chemistry, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2 4 41 Ebara, Shinagawa ku, Tokyo 142 8501, Japan
    Clin Biochem 38:643-53. 2005
    We developed an in vitro model of elastic fiber assembly that provides a comparison of the efficiency of different tropoelastin molecules to organize into fibers.
  23. pmc A novel cell adhesion region in tropoelastin mediates attachment to integrin αVβ5
    Pearl Lee
    From the School of Molecular Bioscience
    J Biol Chem 289:1467-77. 2014
    b>Tropoelastin protein monomers assemble to form elastin. Cellular integrin αVβ3 binds RKRK at the C-terminal tail of tropoelastin...
  24. ncbi Sustained changes in lung expansion alter tropoelastin mRNA levels and elastin content in fetal sheep lungs
    Belinda J Joyce
    Fetal and Neonatal Research Group, Department of Physiology, Monash University, Victoria 3800, Australia
    Am J Physiol Lung Cell Mol Physiol 284:L643-9. 2003
    ..Lung tropoelastin mRNA levels were assessed by Northern blot analysis, total elastin content was measured biochemically, and ..
  25. ncbi Specificity in the coacervation of tropoelastin: solvent exposed lysines
    Steven G Wise
    School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia
    J Struct Biol 149:273-81. 2005
    b>Tropoelastin protein monomers associate by coacervation and are cross-linked in vivo to form elastin macro-assemblies...
  26. pmc Decreased elastin deposition and high proliferation of fibroblasts from Costello syndrome are related to functional deficiency in the 67-kD elastin-binding protein
    A Hinek
    Division of Cardiovascular Research, The Hospital for Sick Children, Toronto, ON M5G 1X8 Canada
    Am J Hum Genet 66:859-72. 2000
    ..obtained from patients with Costello syndrome did not assemble elastic fibers, despite an adequate synthesis of tropoelastin and normal deposition of the microfibrillar scaffold...
  27. ncbi Stability of a therapeutic layer of immobilized recombinant human tropoelastin on a plasma-activated coated surface
    Anna Waterhouse
    School of Molecular Bioscience, University of Sydney, Sydney, NSW 2006, Australia
    Pharm Res 28:1415-21. 2011
    To modify blood-contacting stainless surfaces by covalently coating them with a serum-protease resistant form of tropoelastin (TE)...
  28. ncbi Neutrophil elastase-initiated EGFR/MEK/ERK signaling counteracts stabilizing effect of autocrine TGF-beta on tropoelastin mRNA in lung fibroblasts
    Sandra J DiCamillo
    Department of Biochemistry, Boston University School of Medicine, MA 02118, USA
    Am J Physiol Lung Cell Mol Physiol 291:L232-43. 2006
    ..soluble epidermal growth factor receptor (EGFR) ligands and initiates EGFR/MEK/ERK signaling to downregulate tropoelastin mRNA in neonatal rat lung fibroblasts (DiCamillo SJ, Carreras I, Panchenko MV, Stone PJ, Nugent MA, Foster JA, ..
  29. ncbi Role of polyproline II conformation in human tropoelastin structure
    Brigida Bochicchio
    Laboratory of Protein Chemistry, Department of Chemistry A M Tamburro, University of Basilicata, Potenza, Italy
    Chirality 23:694-702. 2011
    In this review, we present a comprehensive overview of the molecular studies on human tropoelastin domains accomplished by Tamburro and co-workers in the last decade...
  30. pmc Mechanical property characterization of electrospun recombinant human tropoelastin for vascular graft biomaterials
    Kathryn A McKenna
    Oregon Medical Laser Center, Providence St Vincent Medical Center, Portland, OR 97225, USA
    Acta Biomater 8:225-33. 2012
    ..the structural and mechanical properties and the vascular cell compatibility of electrospun recombinant human tropoelastin (rTE) were evaluated as a potential vascular graft support matrix...
  31. ncbi Molecular orientation of tropoelastin is determined by surface hydrophobicity
    Anton P Le Brun
    Bragg Institute, Australian Nuclear Science and Technology Organisation, Locked Bag 2001, Kirrawee DC, NSW 2232, Australia
    Biomacromolecules 13:379-86. 2012
    b>Tropoelastin is the precursor of the extracellular protein elastin and is utilized in tissue engineering and implant technology by adapting the interface presented by surface-bound tropoelastin...
  32. ncbi Coacervation of tropoelastin
    Giselle C Yeo
    School of Molecular Bioscience, The University of Sydney, Australia
    Adv Colloid Interface Sci 167:94-103. 2011
    The coacervation of tropoelastin represents the first major stage of elastic fiber assembly...
  33. ncbi Characterization of an unusual tropoelastin with truncated C-terminus in the frog
    Ming Miao
    Molecular Structure and Function Program, Hospital for Sick Children, Toronto, Canada
    Matrix Biol 28:432-41. 2009
    b>Tropoelastin is the monomeric form of elastin, a major polymeric protein of the extracellular elastic matrix of vertebrate tissues with properties of extensibility and elastic recoil...
  34. ncbi Localizing alpha-helices in human tropoelastin: assembly of the elastin "puzzle"
    Antonio Mario Tamburro
    Department of Chemistry, Universita degli Studi della Basilicata, Via N Sauro, 85, 85100 Potenza, Italy
    Biochemistry 45:9518-30. 2006
    Polyalanine cross-linking domains encoded by exons 6, 15, 17, 19, 21, 23, 25, 27, 29, 31 of human tropoelastin were synthesized, and their conformations were studied in different solutions and at different temperatures by CD and (1)H NMR...
  35. ncbi Elastase-released epidermal growth factor recruits epidermal growth factor receptor and extracellular signal-regulated kinases to down-regulate tropoelastin mRNA in lung fibroblasts
    Sandra J DiCamillo
    Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    J Biol Chem 277:18938-46. 2002
    ..with transient, peaking at 30 min, induction of c-Fos and sustained, observed at 24-48 h, decrease of tropoelastin mRNA levels in NE-challenged cells...
  36. pmc Oxidative modifications of the C-terminal domain of tropoelastin prevent cell binding
    Kamal Akhtar
    Department of Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 286:13574-82. 2011
    b>Tropoelastin (TE), the soluble monomer of elastin, is synthesized by elastogenic cells, such as chondrocytes, fibroblasts, and smooth muscle cells (SMCs)...
  37. pmc Shape of tropoelastin, the highly extensible protein that controls human tissue elasticity
    Clair Baldock
    Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, United Kingdom
    Proc Natl Acad Sci U S A 108:4322-7. 2011
    Elastin enables the reversible deformation of elastic tissues and can withstand decades of repetitive forces. Tropoelastin is the soluble precursor to elastin, the main elastic protein found in mammals...
  38. ncbi The dissection of human tropoelastin: from the molecular structure to the self-assembly to the elasticity mechanism
    Antonio M Tamburro
    Department of Chemistry, Universita della Basilicata, Potenza, Italy
    Pathol Biol (Paris) 53:383-9. 2005
    ..results obtained by analyzing the conformation of polypeptide sequences encoded by the single exons of human tropoelastin demonstrated the presence of labile conformations such as poly-proline II helix (PPII) and beta-turns whose ..
  39. pmc Biomaterials derived from silk-tropoelastin protein systems
    Xiao Hu
    Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA
    Biomaterials 31:8121-31. 2010
    A structural protein blend system based on silkworm silk fibroin and recombinant human tropoelastin is described...
  40. ncbi Degradation of tropoelastin by matrix metalloproteinases--cleavage site specificities and release of matrikines
    Andrea Heinz
    Institute of Pharmacy, Martin Luther University Halle Wittenberg, Halle Saale, Germany
    FEBS J 277:1939-56. 2010
    ..elastin-degrading diseases, the aim of this study was to investigate the degradation of the natural substrate tropoelastin by the elastinolytic matrix metalloproteinases MMP-7, MMP-9, and MMP-12 and to compare the cleavage site ..
  41. ncbi Tropoelastin as a thermodynamically unfolded premolten globule protein: The effect of trimethylamine N-oxide on structure and coacervation
    Leanne B Dyksterhuis
    CSIRO Molecular and Health Technologies, Clayton, VIC 3168, Australia
    Arch Biochem Biophys 487:79-84. 2009
    b>Tropoelastin is the monomer building block of the biopolymer elastin, which is responsible for elasticity in arteries, lung and skin...
  42. pmc Tropoelastin bridge region positions the cell-interactive C terminus and contributes to elastic fiber assembly
    Giselle C Yeo
    School of Molecular Bioscience, University of Sydney, New South Wales 2006, Australia
    Proc Natl Acad Sci U S A 109:2878-83. 2012
    The tropoelastin monomer undergoes stages of association by coacervation, deposition onto microfibrils, and cross-linking to form elastic fibers...
  43. ncbi Dissection of human tropoelastin: exon-by-exon chemical synthesis and related conformational studies
    Antonio M Tamburro
    Department of Chemistry, Universita della Basilicata, Via N Sauro 85, 85100 Potenza, Italy
    Biochemistry 42:13347-62. 2003
    Polypeptide sequences encoding the single exons of human tropoelastin were synthesized and their conformations were studied in different solvents and at different temperatures by CD and (1)H NMR...
  44. ncbi New insights into elastic fiber assembly
    Jessica E Wagenseil
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Birth Defects Res C Embryo Today 81:229-40. 2007
    ....
  45. pmc Differential regulation of elastic fiber formation by fibulin-4 and -5
    Rawshan Choudhury
    Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, United Kingdom
    J Biol Chem 284:24553-67. 2009
    ..We have determined how they interact with tropoelastin, lysyl oxidase, and fibrillin-1, thereby revealing how they differentially regulate assembly...
  46. ncbi Differential expression of two tropoelastin genes in zebrafish
    M Miao
    Cardiovascular Research, Hospital for Sick Children, Toronto, ON, Canada
    Matrix Biol 26:115-24. 2007
    ..Elastin is synthesized as a monomer, tropoelastin, but is rapidly transformed into its final polymeric form in the extracellular matrix...
  47. ncbi Structural determinants of cross-linking and hydrophobic domains for self-assembly of elastin-like polypeptides
    Ming Miao
    Research Institute, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada
    Biochemistry 44:14367-75. 2005
    ..To achieve the required structural durability of the elastic matrix, the elastin monomer, tropoelastin, undergoes ordered assembly into a covalently cross-linked, fibrillar polymeric structure...
  48. ncbi Sequences and domain structures of mammalian, avian, amphibian and teleost tropoelastins: Clues to the evolutionary history of elastins
    Martin I S Chung
    Cardiovascular Research Program, Research Institute, Hospital for Sick Children, Toronto, ON, Canada M5G1X8
    Matrix Biol 25:492-504. 2006
    b>Tropoelastin is the monomeric form of elastin, a polymeric extracellular matrix protein responsible for properties of extensibility and elastic recoil in connective tissues of most vertebrates...
  49. pmc EGF antagonizes TGF-beta-induced tropoelastin expression in lung fibroblasts via stabilization of Smad corepressor TGIF
    Shenghong Yang
    Department of Biochemistry, Boston University School of Medicine, 715 Albany St, Boston, Massachusetts 02118, USA
    Am J Physiol Lung Cell Mol Physiol 295:L143-51. 2008
    ..reported that neutrophil elastase (NE) downregulates transforming growth factor-beta (TGF-beta)-maintained tropoelastin mRNA levels in lung fibroblasts through transactivation of the epidermal growth factor (EGF) receptor (EGFR)/..
  50. ncbi Ultraviolet radiation increases tropoelastin mRNA expression in the epidermis of human skin in vivo
    J Y Seo
    Department of Dermatology, Seoul National University College of Medicine, Seoul National University Hospital, Korea
    J Invest Dermatol 116:915-9. 2001
    ..In this study, it was demonstrated that ultraviolet irradiation induced tropoelastin mRNA expression in the keratinocytes of human skin in vivo and also in cultured human keratinocytes by in situ ..
  51. ncbi Fibulin-5 mutations: mechanisms of impaired elastic fiber formation in recessive cutis laxa
    Qirui Hu
    Department of Pediatrics, Washington University School of Medicine, St Louis, MO 63110, USA
    Hum Mol Genet 15:3379-86. 2006
    ..Purified recombinant fibulin-5 with either mutation showed reduced affinity for tropoelastin in solid-phase binding assays...
  52. ncbi Molecular interactions of biglycan and decorin with elastic fiber components: biglycan forms a ternary complex with tropoelastin and microfibril-associated glycoprotein 1
    Betty Reinboth
    Department of Pathology, University of Adelaide, Adelaide, South Australia 5005, Australia
    J Biol Chem 277:3950-7. 2002
    ..dermatan sulfate proteoglycans biglycan and decorin have been investigated with the elastic fiber components, tropoelastin, fibrillin-containing microfibrils, and microfibril-associated glycoproteins (MAGP) 1 and 2...
  53. ncbi Tropoelastin binding to fibulins, nidogen-2 and other extracellular matrix proteins
    T Sasaki
    Max Planck Institut fur Biochemie, Am Klopferspitz 18a, D 82152, Martinsried, Germany
    FEBS Lett 460:280-4. 1999
    Elastic fibers in vessel walls and other tissues consist of cross-linked tropoelastin in association with several microfibrillar proteins...
  54. pmc Analysis of dermal elastic fibers in the absence of fibulin-5 reveals potential roles for fibulin-5 in elastic fiber assembly
    Jiwon Choi
    Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada
    Matrix Biol 28:211-20. 2009
    ..lower levels of mature cross-linked elastin in the Fbln5(-/-) dermis, however, gene expression levels for tropoelastin and fibrillin-1, the major elastic fiber components, were unaffected...
  55. ncbi Elastic fiber formation: a dynamic view of extracellular matrix assembly using timer reporters
    Beth A Kozel
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Cell Physiol 207:87-96. 2006
    ..the dynamics of elastic fiber assembly, mammalian cells were transfected with a cDNA construct encoding bovine tropoelastin in frame with the Timer reporter...
  56. ncbi DANCE/fibulin-5 promotes elastic fiber formation in a tropoelastin isoform-dependent manner
    Risa Nonaka
    Department of Clinical Chemistry, Hoshi University School of Pharmacy and Pharmaceutical Sciences, Tokyo, Japan
    Clin Biochem 42:713-21. 2009
    ..of fibulin-5 in the elastic fiber formation, we studied the molecular interactions among elastin, fibrillin-1, and fibulin-5 in the extracellular space and the maturation of tropoelastin using retinal pigment epithelial cells (ARPE-19).
  57. ncbi Supramolecular amyloid-like assembly of the polypeptide sequence coded by exon 30 of human tropoelastin
    Antonio Mario Tamburro
    Department of Chemistry, Universita della Basilicata, Via N Sauro 85, 85100 Potenza, Italy
    J Biol Chem 280:2682-90. 2005
    ..similar to those shown by amyloid networks, is shown by the polypeptide sequence encoded by exon 30 of human tropoelastin. To better understand the molecular properties of this sequence to give amyloid fibers, we used CD, NMR, and ..
  58. ncbi Distinct steps of cross-linking, self-association, and maturation of tropoelastin are necessary for elastic fiber formation
    Fumiaki Sato
    Department of Clinical Chemistry, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2 4 41 Ebara, Shinagawa ku, Tokyo 142 8501, Japan
    J Mol Biol 369:841-51. 2007
    Elastic fibers play an important role in the characteristic resilience of many tissues. The assembly of tropoelastin into a fibrillar matrix is a complex stepwise process and the deposition and cross-linking of tropoelastin are believed ..
  59. ncbi The Pro-regions of lysyl oxidase and lysyl oxidase-like 1 are required for deposition onto elastic fibers
    Laetitia Thomassin
    Institut de Biologie et Chimie des Proteines, CNRS Université Lyon 1 UMR 5086, 7 passage du Vercors, 69367 Lyon Cedex 07, France
    J Biol Chem 280:42848-55. 2005
    ..Ligand blot and mammalian two-hybrid assays confirmed an interaction between tropoelastin and the pro-regions of both LOX and LOXL...
  60. pmc Targeted disruption of fibulin-4 abolishes elastogenesis and causes perinatal lethality in mice
    Precious J McLaughlin
    Department of Ophthalmology and Vision Science, University of Arizona, Tucson, AZ 85711, USA
    Mol Cell Biol 26:1700-9. 2006
    ..However, expression of tropoelastin or lysyl oxidase mRNA was unaffected in fibulin-4-/- mice...
  61. ncbi Fibrillin-1 interactions with heparin. Implications for microfibril and elastic fiber assembly
    Stuart A Cain
    Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, United Kingdom
    J Biol Chem 280:30526-37. 2005
    ..cell attachment, but heparin and MAGP-1 competed for binding to the fibrillin-1 N terminus, and heparin and tropoelastin competed for binding to a central fibrillin-1 sequence...
  62. ncbi Tropoelastin massively associates during coacervation to form quantized protein spheres
    Adam W Clarke
    School of Molecular and Microbial Biosciences G08, University of Sydney, New South Wales 2006, Australia
    Biochemistry 45:9989-96. 2006
    b>Tropoelastin, the precursor of elastin, undergoes a rapid monomer to multimer association in an inverse temperature transition. This association culminates in the rapid formation of stable, optically distinct droplets of tropoelastin...
  63. ncbi Tropoelastin interacts with cell-surface glycosaminoglycans via its COOH-terminal domain
    Thomas J Broekelmann
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 280:40939-47. 2005
    ..and cell biological approach, we have identified a cell interaction site at the carboxyl terminus of tropoelastin. Cell interactions with the COOH-terminal sequence are not through the elastin-binding protein (EBP67) because ..
  64. pmc Fibulin-5/DANCE has an elastogenic organizer activity that is abrogated by proteolytic cleavage in vivo
    Maretoshi Hirai
    Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    J Cell Biol 176:1061-71. 2007
    ..We report that fibulin-5 protein potently induces elastic fiber assembly and maturation by organizing tropoelastin and cross-linking enzymes onto microfibrils...
  65. ncbi Coacervation is promoted by molecular interactions between the PF2 segment of fibrillin-1 and the domain 4 region of tropoelastin
    Adam W Clarke
    School of Molecular and Microbial Biosciences G08, University of Sydney, Sydney, New South Wales, 2006, Australia
    Biochemistry 44:10271-81. 2005
    In forming elastic fibers, microfibrils act as the scaffold sites for depositing the elastin precursor tropoelastin. We examined key binding interactions that promote massive tropoelastin association through coacervation...
  66. ncbi A comparative analysis of the fibulin protein family. Biochemical characterization, binding interactions, and tissue localization
    Naoyuki Kobayashi
    Max Planck Institut fur Biochemie, D 82152 Martinsried, Germany
    J Biol Chem 282:11805-16. 2007
    ..Binding to tropoelastin was strong for fibulin-2 and -5, moderate for fibulin-4 and -1, and relatively weak for fibulin-3...
  67. ncbi Autosomal dominant cutis laxa with severe lung disease: synthesis and matrix deposition of mutant tropoelastin
    Zsolt Urban
    Department of Anatomy, University of Hawaii, Honolulu, Hawaii, USA
    J Invest Dermatol 124:1193-9. 2005
    ..In addition to the normal 68 kDa tropoelastin (TE) protein, an abnormal, 120 kDa polypeptide was detected in the proband and her affected daughter in a CL ..
  68. ncbi Fibrillin-1 interactions with fibulins depend on the first hybrid domain and provide an adaptor function to tropoelastin
    Ehab El-Hallous
    Faculty of Medicine, Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec H3A 2B2, Canada
    J Biol Chem 282:8935-46. 2007
    ..Formation of ternary complexes between fibrillin-1, fibulins, and tropoelastin demonstrated that fibulin-2 and -5 but much less fibulin-4, are able to act as molecular adaptors between ..
  69. ncbi Microfibril-associated glycoprotein-1 and fibrillin-2 are associated with tropoelastin deposition in vitro
    Eichi Tsuruga
    Department of Oral Anatomy, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari Tobetsu, Hokkaido 061 0293, Japan
    Int J Biochem Cell Biol 37:120-9. 2005
    Elastic system fibers consist of microfibrils and tropoelastin. During development, microfibrils act as a template on which tropoelastin is deposited...
  70. ncbi Retrovirally mediated overexpression of versican v3 by arterial smooth muscle cells induces tropoelastin synthesis and elastic fiber formation in vitro and in neointima after vascular injury
    Mervyn J Merrilees
    Department of Anatomy with Radiology, The University of Auckland, Auckland, New Zealand
    Circ Res 90:481-7. 2002
    ..We now report that V3-overexpressing ASMCs exhibit significantly increased expression of tropoelastin and increased formation of elastic fibers in long-term cell cultures...
  71. ncbi Fibroblast tropoelastin and alpha-smooth-muscle actin expression are repressed by particulate-activated macrophage-derived tumor necrosis factor-alpha in experimental silicosis
    T J Mariani
    Department of Internal Medicine, Washington University School of Medicine at Barnes Jewish Hospital, St Louis, Missouri, USA
    Am J Respir Cell Mol Biol 21:185-92. 1999
    ..Reinitiated tropoelastin expression was identified throughout the lung in fibroblasts expressing alpha-smooth-muscle actin, whereas ..
  72. ncbi Fibrillins, fibulins, and matrix-associated glycoprotein modulate the kinetics and morphology of in vitro self-assembly of a recombinant elastin-like polypeptide
    Judith T Cirulis
    Research Institute, The Hospital for Sick Children, Toronto, Canada
    Biochemistry 47:12601-13. 2008
    ..Recent data suggest the complex interaction of tropoelastin, the monomeric form of elastin, with a number of other elastic matrix-associated proteins, including fibrillins,..
  73. ncbi In vitro degradation of human tropoelastin by MMP-12 and the generation of matrikines from domain 24
    Samuel Taddese
    Institute of Pharmacy, Martin Luther University Halle Wittenberg, Halle Saale, Germany
    Matrix Biol 28:84-91. 2009
    ..b>Tropoelastin is the precursor of the cross-linked elastin and its expression is triggered by elastin-degrading factors as a ..
  74. ncbi Sequential loss of two neighboring exons of the tropoelastin gene during primate evolution
    Z Szabo
    Pacific Biomedical Research Center, University of Hawaii, Honolulu, HI 96822, USA
    J Mol Evol 49:664-71. 1999
    Previous evidence has demonstrated the absence of exons 34 and 35 within the 3' end of the human tropoelastin (ELN) gene...
  75. ncbi Tropoelastin expression by periodontal fibroblasts
    E Tsuruga
    Department of Oral Anatomy, School of Dentistry, Health Sciences University of Hokkaido, Ishikari Tobetsu, Japan
    J Dent Res 81:198-202. 2002
    ..We examined gene expression and accumulation of tropoelastin in the cell-matrix layers of human gingival fibroblasts (HGF) and periodontal ligament fibroblasts (HPLF) in ..
  76. pmc Loss-of-function mutations in ATP6V0A2 impair vesicular trafficking, tropoelastin secretion and cell survival
    Vishwanathan Hucthagowder
    Department of Pediatrics, Washington University School of Medicine, 660 South Euclid Avenue, Campus Box 8208, St Louis, MO 63110, USA
    Hum Mol Genet 18:2149-65. 2009
    ..Immunostaining of ARCL2 cells showed the accumulation of tropoelastin (TE) in the Golgi and in large, abnormal intracellular and extracellular aggregates...
  77. ncbi Hydrophobic domains of human tropoelastin interact in a context-dependent manner
    P Toonkool
    Department of Biochemistry, University of Sydney, Sydney, New South Wales 2006, Australia
    J Biol Chem 276:44575-80. 2001
    b>Tropoelastin is the soluble precursor of elastin, the major component of the extracellular elastic fiber...
  78. ncbi Protein interaction studies of MAGP-1 with tropoelastin and fibrillin-1
    S A Jensen
    Department of Biochemistry G08, University of Sydney, New South Wales 2006, Australia
    J Biol Chem 276:39661-6. 2001
    ..To investigate the interaction of MAGP-1 with tropoelastin and fibrillin-1, we expressed human MAGP-1 as a T7-tag fusion protein in Escherichia coli...
  79. ncbi Building Elastin. Incorporation of recombinant human tropoelastin into extracellular matrices using nonelastogenic rat-1 fibroblasts as a source for lysyl oxidase
    P J Stone
    Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    Am J Respir Cell Mol Biol 24:733-9. 2001
    The purpose of this study was to assess the feasibility of crosslinking exogenously produced tropoelastin, the precursor of insoluble elastin, into existing elastin...
  80. ncbi The cysteine residues in the carboxy terminal domain of tropoelastin form an intrachain disulfide bond that stabilizes a loop structure and positively charged pocket
    P L Brown
    Department of Cell Biology, Jewish Hospital, Washington University Medical Center, St Louis, MO 63110
    Biochem Biophys Res Commun 186:549-55. 1992
    Analysis of purified bovine tropoelastin with Ellman's reagent and [14C]iodoacetamide demonstrated that the only two cysteine residues in the molecule form an intrachain disulfide bond...
  81. ncbi Heparan sulfate regulates fibrillin-1 N- and C-terminal interactions
    Stuart A Cain
    Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, United Kingdom
    J Biol Chem 283:27017-27. 2008
    ..by exons 59-62 near the fibrillin-1 C terminus are novel conformation-dependent high affinity heparin and tropoelastin binding sites...
  82. pmc Identification of tropoelastin as a ligand for the 65-kD FK506-binding protein, FKBP65, in the secretory pathway
    E C Davis
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Cell Biol 140:295-303. 1998
    The folding and trafficking of tropoelastin is thought to be mediated by intracellular chaperones, although the identity and role of any tropoelastin chaperone remain to be determined...
  83. ncbi Glycosaminoglycan-mediated coacervation of tropoelastin abolishes the critical concentration, accelerates coacervate formation, and facilitates spherule fusion: implications for tropoelastin microassembly
    Yidong Tu
    School of Molecular and Microbial Biosciences G08, University of Sydney, NSW 2006, Australia
    Biomacromolecules 9:1739-44. 2008
    Elastogenesis and elastin repair depend on the secretion of tropoelastin from the cell, yet cellular production is low in the many biological systems that have been studied...
  84. ncbi Molecular basis of elastic fiber formation. Critical interactions and a tropoelastin-fibrillin-1 cross-link
    Matthew J Rock
    Wellcome Trust Centre for Cell Matrix Research, School of Biological Sciences, University of Manchester, 2 205 Stopford Building, Oxford Road, Manchester M13 9PT, United Kingdom
    J Biol Chem 279:23748-58. 2004
    ..binding of two overlapping fibrillin-1 fragments (encoded by central exons 18-25 and 24-30) to tropoelastin, which, in microfibrils, map to an exposed "arms" feature adjacent to the beads...
  85. ncbi Interaction of tropoelastin with the amino-terminal domains of fibrillin-1 and fibrillin-2 suggests a role for the fibrillins in elastic fiber assembly
    T M Trask
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 275:24400-6. 2000
    Alignment of tropoelastin molecules during the process of elastogenesis is thought to require fibrillin-containing microfibrils...
  86. ncbi Characterization of the molecular interaction between tropoelastin and DANCE/fibulin-5
    Hiroshi Wachi
    Department of Clinical Chemistry, Hoshi University School of Pharmacy and Pharmaceutical Sciences, 2 4 41 Ebara, Shinagawa ku, Tokyo 142 8501, Japan
    J Biochem 143:633-9. 2008
    ..The present experiments were carried out to characterize the molecular interaction between fibulin-5 and tropoelastin. Our data showed that the divalent cations of Ca(2+), Ba(2+) and Mg(2+) significantly enhanced the binding of ..
  87. ncbi Heparan sulphate interacts with tropoelastin, with some tropoelastin peptides and is present in human dermis elastic fibers
    Dealba Gheduzzi
    Department of Biomedical Sciences, University of Modena and Reggio Emilia, Via Campi 287, 41100 Modena, Italy
    Matrix Biol 24:15-25. 2005
    ..with normal elastic fibers in human dermis and that isolated HS chains interact in vitro with recombinant tropoelastin and with peptides encoded by distinct exons of the human tropoelastin gene (EDPs)...
  88. ncbi Gene expression of tropoelastin is enhanced in the aorta proximal to the coarctation in rabbits
    Chengpei Xu
    Department of Surgery, Stanford University, Division of Vascular Surgery, 300 Pasteur Drive, Suite H3600, Stanford, California, USA
    Exp Mol Pathol 72:115-23. 2002
    ..in the aortic wall in response to acute elevation of blood pressure, we studied the aortic gene expression of tropoelastin in a rabbit midthoracic aortic coarctation model...
  89. ncbi Heterogeneity of rat tropoelastin mRNA revealed by cDNA cloning
    R A Pierce
    Department of Surgery, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, New Brunswick 08903 0019
    Biochemistry 29:9677-83. 1990
    ..gt11 library constructed from poly(A+) RNA isolated from aortic tissue of neonatal rats was screened for rat tropoelastin cDNAs. The first screen, utilizing a human tropoelastin cDNA clone, provided rat tropoelastin cDNAs spanning 2...
  90. ncbi Action of tropoelastin and synthetic elastin sequences on vascular tone and on free Ca2+ level in human vascular endothelial cells
    G Faury
    Laboratoire de Bioénergétique Fondamentale et Appliquée, Universite Joseph Fourier, Grenoble, France
    Circ Res 82:328-36. 1998
    ..we have compared the action of different concentrations (10(-13) to 10(-7) mol/L) of recombinant human tropoelastin, eight synthetic elastin peptides, and a control peptide (VPVGGA) on both rat aortic ring tension and [Ca2+]i ..
  91. ncbi Use of an intron polymorphism to localize the tropoelastin gene to mouse chromosome 5 in a region of linkage conservation with human chromosome 7
    K S Wydner
    Department of Biological Sciences, Rutgers University, Piscataway, New Jersey 08855 1059
    Genomics 23:125-31. 1994
    The complete coding sequence for mouse tropoelastin was obtained from overlapping reverse transcriptase polymerase chain reaction (PCR) amplimers...
  92. ncbi Induction of fibulin-5 gene is regulated by tropoelastin gene, and correlated with tropoelastin accumulation in vitro
    Eichi Tsuruga
    Department of Oral Anatomy, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari Tobetsu, Hokkaido 061 0293, Japan
    Int J Biochem Cell Biol 36:395-400. 2004
    ..examined the relationship between the gene expression of fibulin-5 and the gene expression and accumulation of tropoelastin by comparing elastin-producing cells (human gingival fibroblasts) with non-elastin-producing cells (human ..
  93. ncbi Fibrillin-1 and fibrillin-2 show temporal and tissue-specific regulation of expression in developing elastic tissues
    M C Mariencheck
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Connect Tissue Res 31:87-97. 1995
    ..these possibilities, the expression of fibrillin-1 and fibrillin-2 was compared with expression of MAGP and tropoelastin in two elastogenic tissues that undergo different developmental programs...
  94. ncbi Significant decrease in tropoelastin gene expression in fibroblasts from a Japanese Costello syndrome patient with impaired elastogenesis and enhanced proliferation
    Yutaka Tatano
    Department of Medicinal Biotechnology, Institute for Medicinal Resources, Graduate School of Pharmaceutical Sciences, The University of Tokushima, Tokushima 770 8505
    J Biochem 140:193-200. 2006
    ..In this study we demonstrated a significant decrease in the expression of tropoelastin mRNA in fibroblasts derived from a Japanese Costello syndrome patient with impaired elastogenesis and enhanced ..
  95. ncbi Disordered pulmonary myofibroblast distribution and elastin expression in preterm infants with Ureaplasma urealyticum pneumonitis
    Rose Viscardi
    Department of Pediatrics, University of Maryland School of Medicine, 22 South Greene Street, Baltimore, 21201, USA
    Pediatr Dev Pathol 9:143-51. 2006
    ..and transforming growth factor beta1 (TGFbeta1), Hart's elastin staining, and in situ hybridization for tropoelastin (TE) expression...
  96. ncbi Dissection of human tropoelastin: supramolecular organization of polypeptide sequences coded by particular exons
    Antonietta Pepe
    Department of Chemistry, Universita della Basilicata, Via N Sauro 85, 85100 Potenza, Italy
    Matrix Biol 24:96-109. 2005
    Polypeptide sequences encoded by some exons of the human tropoelastin gene (EDP, elastin-derived peptide) have been analysed for their ability to coacervate and to self-assembly...
  97. ncbi Elastogenesis in human arterial disease: a role for macrophages in disordered elastin synthesis
    Alexandra Krettek
    Leducq Center for Cardiovascular Research, Cardiovascular Division, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, 221 Longwood Avenue, Eugene Braunwald Research Center 307, Boston, Mass 02115, USA
    Arterioscler Thromb Vasc Biol 23:582-7. 2003
    ..However, elastogenesis in arterial diseases has received little attention. In this work we hypothesized that disordered elastogenesis is active in matrix remodeling in atheroma and abdominal aortic aneurysm (AAA)...
  98. pmc Aldosterone induces elastin production in cardiac fibroblasts through activation of insulin-like growth factor-I receptors in a mineralocorticoid receptor-independent manner
    Severa Bunda
    Cardiovascular Research Program, University of Toronto, Ontario, Canada
    Am J Pathol 171:809-19. 2007
    ..Most importantly, we demonstrated that aldosterone also increases elastin mRNA levels, tropoelastin synthesis, and elastic fiber deposition in a dose-dependent manner...
  99. ncbi High copper levels and increased elastolysis in a patient with cutis marmorata teleangiectasia congenita
    Aleksander Hinek
    Division of Cardiovascular Research, The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada
    Am J Med Genet A 146:2520-7. 2008
    ..cultures (passage one) of dermal fibroblasts derived from this patient were capable of normal synthesis of tropoelastin, but their net deposition of mature elastic fibers was significantly diminished as compared with cultures of ..
  100. pmc VE-statin/egfl7 regulates vascular elastogenesis by interacting with lysyl oxidases
    Etienne Lelievre
    CNRS, UMR 8161, Institut de Biologie de Lille, Equipe labellisée La Ligue 2005, Universite de Lille I, Universite de Lille II, Institut Pasteur de Lille, Lille, France
    EMBO J 27:1658-70. 2008
    ..the presence of VE-statin/egfl7 were unable to deposit elastic fibres due to a deficient conversion of soluble tropoelastin into insoluble mature elastin...
  101. ncbi Mapping of macrophage elastase cleavage sites in insoluble human skin elastin
    Samuel Taddese
    Institute of Pharmacy, Martin Luther University Halle Wittenberg, Halle Saale, Germany
    Matrix Biol 27:420-8. 2008
    ..The amino acid preferences of the different subsites on the catalytic domain of MMP-12 were analyzed...

Research Grants43

  1. The role of the extracellular biophysical and biomechanical milieu in CHDs
    Lauren D Black; Fiscal Year: 2013
    ..Decreased cardiac tropoelastin and procollagen gene expression and decreased left ventricular function in LHH compared to normal hearts ..
  2. Cues for cell-mediated regeneration of elastin matrix in aortic aneurysms
    Anand Ramamurthi; Fiscal Year: 2012
    ..wherein RASMCs (healthy and aneurysmal) will be provided cues to both synthesize, and assemble and crosslink tropoelastin precursors...
  3. Pulmonary Hypertension in Genetically Modified Mice
    Marlene Rabinovitch; Fiscal Year: 2013
    ..we show, for the first time, that BMPRII ligands, in particular BMP4, can increase mRNA and protein levels of tropoelastin, the soluble precursor of elastin, in cultured human (h) PA SMC...
  4. 2011 Elastin and Elastic Fibers GRS GRC
    Richard A Pierce; Fiscal Year: 2011
    ..latest findings on the protein structure of elastic fiber components including fibrilins, fibulins, LTBPs and tropoelastin monomers and their assembly in vitro and in vivo...
  5. Disorganization of Elastin Matrix Mediates Erectile Dysfunction
    KANCHAN CHITALEY SCHINDLAUER; Fiscal Year: 2013
    ..By maturity, assembly of elastic fibers is complete, and synthesis of new tropoelastin, the soluble precursor, has declined...
  6. Elastase Injury of Pulmonary Extracellular Matrix
    Matthew A Nugent; Fiscal Year: 2012
    ..to inhibit elastase and that elastase leads to increased nuclear HSPGs, reduced histone acetylation, reduced tropoelastin expression, and release of VEGF fragments...
  7. Mouse Models for Elastic Fiber Diseases
    MON LI H CHU; Fiscal Year: 2010
    ..This protein interacts with tropoelastin and fibrillin-1, and its deficiency results in impaired elastic fiber formation and increased TGF-2 signaling ..
  8. Development of a Microstructurally Inspired and Compliance Matched Tissue Enginee
    JONATHAN PIETER VANDE GEEST; Fiscal Year: 2013
    ..proposal is that a TEVG composed of alternating layers of SMC embedded collagen/fibrin and cross-linked human tropoelastin will result in a vascular substitute that can be compliance-matched to that of a native porcine coronary artery...
  9. Multifunctional Tropoelastin-Silk Biomaterial Systems
    David L Kaplan; Fiscal Year: 2013
    ..The hypothesis is that combinations of a highly elastic and dynamic structural protein (tropoelastin) with tough, durable proteins (silk) will generate new multifunctional protein composite systems that can offer ..
  10. INTRACELLULAR TRAFFICKING OF TROPOELASTIN
    Frederick Grinnell; Fiscal Year: 2002
    Elastic fiber assembly is a complicated process that involves the organization of tropoelastin monomers on a microfibril scaffold and the subsequent crosslinking of these monomers into an insoluble elastin matrix...
  11. GLUCOCORTICOID REGULATION OF LUNG TROPOELASTIN
    Richard Pierce; Fiscal Year: 1999
    ..We have identified glucocorticoids as transcriptional activators of fetal lung tropoelastin expression...
  12. EXTRACELLULAR MATRIX REPAIR IN PULMONARY EMPHYSEMA
    Steven Shapiro; Fiscal Year: 2002
    ..Elastic fiber assembly is complex requiring tropoelastin monomers to be aligned on a scaffold of potentially several microfibril proteins where they are then cross-..
  13. Bioengineering & Biologic Studies of Aneurysm Weakening
    David Vorp; Fiscal Year: 2009
    ..to either degradation or maintenance of wall integrity, such as matrix metalloproteinases and ECM precursors tropoelastin and procollagen. The results of this study could have an immediate impact on the clinical management of AAA...
  14. Regulation of Vascular Wall Extracellular Matrix
    William Parks; Fiscal Year: 2006
    ..Elastin is a covalently crosslinked polymer of tropoelastin, the secreted precursor protein, which are assembled into fibers on a microfibrillar scaffold...
  15. CHARACTERIZATION OF TROPOELASTIN ISOFORMS
    William Parks; Fiscal Year: 1992
    b>Tropoelastin is the polypeptide precursor of elastin, a cross-linked fibrous protein essential to the structure and function of lung, large blood vessels and other tissues...
  16. ELASTIC MICROFIBRILS
    Joel Rosenbloom; Fiscal Year: 1999
    ..pattern of expression of the two forms of LTBP-2, (3) to investigate binding interactions between LTBP-2 and tropoelastin, other microfibrillar proteins and TGF-beta and to determine the role of LTBP-2 in elastic fiber assembly...
  17. ASSEMBLY OF LUNG ELASTIN
    Robert Mecham; Fiscal Year: 2009
    ..Our specific aims focus on: 1) Characterizing sequences within tropoelastin that mediate its association with microfibrils and promote self-assembly;2) Elucidating domains within the ..
  18. ELASTIN METABOLISM
    Robert Rucker; Fiscal Year: 1991
    ..We will examine this response as a means of assessing to what extent, the expression of tropoelastin isoforms is altered in developmental versus disease models...
  19. POSTTRANSCRIPTIONAL REGULATION OF TROPOELASTIN EXPRESSIO
    William Parks; Fiscal Year: 2001
    ..Elastin, the principal component of elastic fibers, is the product of crosslinked tropoelastin monomers...
  20. STRUCTURE, FUNCTION AND PATHOLOGY OF ELASTIN
    DAN URRY; Fiscal Year: 1991
    ..sequences containing the repeats, multicomponent models comprised of the repeats, and composities approaching tropoelastin, and 2) to develop three dimensional structures for the peptides for the purpose of understanding the molecular ..
  21. ELASTIC FIBER STRUCTURE, FIBROGENESIS DISEASE
    JUDITH FOSTER; Fiscal Year: 1993
    ..Using chick aortic tissue and cells, the syntheses of tropoelastin and microfibril will be examined at transcriptional, translational and post-translational levels...
  22. ANEURYSM RESEARCH CORE COLLABORATIVE R01
    Robert Thompson; Fiscal Year: 2002
    ..vascular smooth muscle cells in culture, they will specifically evaluate the molecular pathways controlling tropoelastin gene expression and tropoelastin mRNA stability, as well as the regulation of additional gene products involved ..
  23. REGULATED EXPRESSION OF COLLAGENASES IN AAA
    Robert Thompson; Fiscal Year: 2002
    ..vascular smooth muscle cells in culture, they will specifically evaluate the molecular pathways controlling tropoelastin gene expression and tropoelastin mRNA stability, as well as the regulation of additional gene products involved ..
  24. OXIDATIVE MECHANISMS OF AAA FORMATION (COLLABORATIVE R01
    Jay Heinecke; Fiscal Year: 2002
    ..vascular smooth muscle cells in culture, they will specifically evaluate the molecular pathways controlling tropoelastin gene expression and tropoelastin mRNA stability, as well as the regulation of additional gene products involved ..
  25. 2005 Elastin and Elastic Fibers Gordon Conference
    CATHERINE KIELTY; Fiscal Year: 2005
    ..dramatically in recent years, with many major recent findings on the assembly, structure and function of tropoelastin, fibrillins and associated proteins, the role of elastic fibers in development and other biological processes, ..
  26. MECHANISMS FOR CHRONIC UVR-INDUCED DAMAGE TO SKIN
    IRENE KOCHEVAR; Fiscal Year: 2003
    ..The processes involved in stimulating synthesis of tropoelastin by fibroblasts in response to UVR are only inadequately understood...
  27. FIBROBLAST HETEROGENEITY IN PULMONARY FIBROSIS
    RICHARD PAINTER; Fiscal Year: 1991
    ..We will examine expression of the genes responsible for synthesis of collagens, tropoelastin, and fibronectin and for expression of fibronectin receptors...
  28. The Role of Fibulin-5 in Cardiovascular Remodeling
    Hiromi Yanagisawa; Fiscal Year: 2007
    ..Solid-phase binding and immunolocalization studies have indicated that fibulin-5 is a Ca2+ -dependent tropoelastin binding protein...
  29. CELL DIVERSITY IN PULMONARY VASCULAR DEVELOPMENT
    Robert Mecham; Fiscal Year: 2003
    ..In this application, the primary investigator will investigate the hypothesis that tropoelastin and its associated microfibrillar proteins play an important role in smooth muscle cell maturation by providing ..
  30. CHRONIC LUNG INJURY AFTER PREMATURE BIRTH
    Richard Bland; Fiscal Year: 2003
    ....
  31. MAGP: A Matricellular Protein with Multiple Functions
    Robert Mecham; Fiscal Year: 2007
    ..4. To characterize the promoter sequences of the MAGP1 gene and to identify the critical regions for tissue expression and regulation during cell differentiation. ..
  32. Functional Repair of Elastin with Recomb. Tropoelastin
    Phillip Stone; Fiscal Year: 2004
    ..this goal our objectives are to regenerate elastin in tissue culture systems by crosslinking recombinant tropoelastin (rTE) into damaged elastin and to characterize the effectiveness of this regeneration by assessing the ..
  33. The AVS2004 Biomaterials Interfaces Symposium
    Ashutosh Chilkoti; Fiscal Year: 2004
    ..abstract_text> ..
  34. Effect of diflunisal (IND68092) on familial amyloidosis
    JOHN BERK; Fiscal Year: 2009
    ..poiuytrewq c Diflunisal drug levels will be determined to assess treatment compliance. We will correlate FAP disease progression with TTR stabilization. ..
  35. X-RAY DIFFRACTION STUDIES OF OLIGOPEPTIDES
    Isabella Karle; Fiscal Year: 2007
    ..Among X-ray quality crystals on hand are peptides with gamma-amino residues and some with probable multi-stranded beta sheets. ..
  36. Stretch and Vitamin A Effects on Alveolar Formation
    KURT ALBERTINE; Fiscal Year: 2009
    ..We will test the hypothesis that the promoter regions of our 3 target genes are retinoid responsive. Thus, this project will provide novel mechanistic insights about alveolar formation and its dysregulation in CLD. ..
  37. Elastin Gene Mutations: Mechanisms Causing SVAS and ADCL
    Zsolt Urban; Fiscal Year: 2005
    ..In ADCL patients, in contrast, we have identified mutations that result in the expression of mutant tropoelastin. Based on these results, we hypotesize that different classes of elastin gene mutations result in SVAS and ADCL ..
  38. MMP-2 in Neonatal Hypoxic Pulmonary Vascular Remodeling
    Namasivayam Ambalavanan; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  39. C-Reactive Protein in Extremely Low Birth Weight Neonates
    Namasivayam Ambalavanan; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  40. Nanophotonics for Select Agent Detection
    Ashutosh Chilkoti; Fiscal Year: 2007
    ..abstract_text> ..
  41. 2006 Biointerface Science
    Ashutosh Chilkoti; Fiscal Year: 2006
    ..By providing support for this conference, NIBIB will facilitate the participation of young faculty and other participants in this unique scientific forum that will positively impact their future careers. [unreadable]..