Top Publications

  1. ncbi Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis
    Jesper V Olsen
    Department of Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Am Klopferspitz 18, D 82152 Martinsried near Munich, Germany
    Sci Signal 3:ra3. 2010
  2. ncbi The Hippo signaling pathway coordinately regulates cell proliferation and apoptosis by inactivating Yorkie, the Drosophila Homolog of YAP
    Jianbin Huang
    Department of Physiology, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
    Cell 122:421-34. 2005
  3. ncbi p63 is essential for regenerative proliferation in limb, craniofacial and epithelial development
    A Yang
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 398:714-8. 1999
  4. ncbi TSC2 is phosphorylated and inhibited by Akt and suppresses mTOR signalling
    Ken Inoki
    Department of Biological Chemistry, University of Michigan Medical School, 1301 Catherine Road, Ann Arbor, MI 48109, USA
    Nat Cell Biol 4:648-57. 2002
  5. ncbi p63 is a p53 homologue required for limb and epidermal morphogenesis
    A A Mills
    Howard Hughes Medical Institute, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Nature 398:708-13. 1999
  6. ncbi p63 Is essential for the proliferative potential of stem cells in stratified epithelia
    Makoto Senoo
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell 129:523-36. 2007
  7. pmc IFI16 is an innate immune sensor for intracellular DNA
    Leonie Unterholzner
    School of Biochemistry and Immunology, Trinity College Dublin, Dublin, Ireland
    Nat Immunol 11:997-1004. 2010
  8. ncbi Cohesins: chromosomal proteins that prevent premature separation of sister chromatids
    C Michaelis
    Research Institute of Molecular Pathology, Vienna, Austria
    Cell 91:35-45. 1997
  9. pmc Occludin: a novel integral membrane protein localizing at tight junctions
    M Furuse
    Department of Information Physiology, National Institute for Physiological Sciences, Aichi, Japan
    J Cell Biol 123:1777-88. 1993
  10. pmc TEAD mediates YAP-dependent gene induction and growth control
    Bin Zhao
    Department of Pharmacology and Moores Cancer Center, University of California at San Diego, La Jolla, California 92093, USA
    Genes Dev 22:1962-71. 2008

Detail Information

Publications376 found, 100 shown here

  1. ncbi Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis
    Jesper V Olsen
    Department of Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Am Klopferspitz 18, D 82152 Martinsried near Munich, Germany
    Sci Signal 3:ra3. 2010
    ..In particular, nuclear proteins and proteins involved in regulating metabolic processes have high phosphorylation site occupancy in mitosis. This suggests that these proteins may be inactivated by phosphorylation in mitotic cells...
  2. ncbi The Hippo signaling pathway coordinately regulates cell proliferation and apoptosis by inactivating Yorkie, the Drosophila Homolog of YAP
    Jianbin Huang
    Department of Physiology, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
    Cell 122:421-34. 2005
    ..Thus, Yki is a critical target of the Wts/Lats protein kinase and a potential oncogene...
  3. ncbi p63 is essential for regenerative proliferation in limb, craniofacial and epithelial development
    A Yang
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 398:714-8. 1999
    ..Taken together, our results indicate that p63 is critical for maintaining the progenitor-cell populations that are necessary to sustain epithelial development and morphogenesis...
  4. ncbi TSC2 is phosphorylated and inhibited by Akt and suppresses mTOR signalling
    Ken Inoki
    Department of Biological Chemistry, University of Michigan Medical School, 1301 Catherine Road, Ann Arbor, MI 48109, USA
    Nat Cell Biol 4:648-57. 2002
    ..Our data indicate a molecular mechanism for TSC2 in insulin signalling, tumour suppressor functions and in the inhibition of cell growth...
  5. ncbi p63 is a p53 homologue required for limb and epidermal morphogenesis
    A A Mills
    Howard Hughes Medical Institute, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Nature 398:708-13. 1999
    ..Thus, in contrast to p53, p63 is essential for several aspects of ectodermal differentiation during embryogenesis...
  6. ncbi p63 Is essential for the proliferative potential of stem cells in stratified epithelia
    Makoto Senoo
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell 129:523-36. 2007
    ..Together, these data identify p63 as a key, lineage-specific determinant of the proliferative capacity in stem cells of stratified epithelia...
  7. pmc IFI16 is an innate immune sensor for intracellular DNA
    Leonie Unterholzner
    School of Biochemistry and Immunology, Trinity College Dublin, Dublin, Ireland
    Nat Immunol 11:997-1004. 2010
    ..IFI16 (p204) is the first PYHIN protein to our knowledge shown to be involved in IFN-β induction. Thus, the PYHIN proteins IFI16 and AIM2 form a new family of innate DNA sensors we call 'AIM2-like receptors' (ALRs)...
  8. ncbi Cohesins: chromosomal proteins that prevent premature separation of sister chromatids
    C Michaelis
    Research Institute of Molecular Pathology, Vienna, Austria
    Cell 91:35-45. 1997
    ..Proteins homologous to Scc1p exist in a variety of eukaryotic organisms including humans. A common cohesion apparatus might be used by all eukaryotic cells during both mitosis and meiosis...
  9. pmc Occludin: a novel integral membrane protein localizing at tight junctions
    M Furuse
    Department of Information Physiology, National Institute for Physiological Sciences, Aichi, Japan
    J Cell Biol 123:1777-88. 1993
    ..These findings revealed that an integral membrane protein localizing at tight junctions is now identified, which we designated as "occludin."..
  10. pmc TEAD mediates YAP-dependent gene induction and growth control
    Bin Zhao
    Department of Pharmacology and Moores Cancer Center, University of California at San Diego, La Jolla, California 92093, USA
    Genes Dev 22:1962-71. 2008
    ..Our study reveals TEAD as a new component in the Hippo pathway playing essential roles in mediating biological functions of YAP...
  11. pmc Phospho.ELM: a database of phosphorylation sites--update 2011
    Holger Dinkel
    SCB Unit, EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany
    Nucleic Acids Res 39:D261-7. 2011
    ..Finally, special emphasis has been put on linking to external resources such as interaction networks and other databases...
  12. pmc Identification and validation of oncogenes in liver cancer using an integrative oncogenomic approach
    Lars Zender
    Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
    Cell 125:1253-67. 2006
    ..Our results establish a tractable model of liver cancer, identify two oncogenes that cooperate by virtue of their coamplification in the same genomic locus, and suggest an efficient strategy for the annotation of human cancer genes...
  13. pmc Transforming properties of YAP, a candidate oncogene on the chromosome 11q22 amplicon
    Michael Overholtzer
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 103:12405-10. 2006
  14. ncbi Akt phosphorylates the Yes-associated protein, YAP, to induce interaction with 14-3-3 and attenuation of p73-mediated apoptosis
    Subham Basu
    Signal Transduction, Cancer Research UK London Research Institue, 44 Lincoln s Inn Fields, London WC2A 3PX, United Kingdom
    Mol Cell 11:11-23. 2003
    ..Akt phosphorylation of YAP may thus suppress the induction of the proapoptotic gene expression response following cellular damage...
  15. ncbi Thematic review series: adipocyte biology. The perilipin family of structural lipid droplet proteins: stabilization of lipid droplets and control of lipolysis
    Dawn L Brasaemle
    Department of Nutritional Sciences and the Rutgers Center for Lipid Research, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA
    J Lipid Res 48:2547-59. 2007
    ..A model is discussed whereby perilipin serves as a dynamic scaffold to coordinate the access of enzymes to the lipid droplet in a manner that is responsive to the metabolic status of the adipocyte...
  16. pmc Trex1 prevents cell-intrinsic initiation of autoimmunity
    Daniel B Stetson
    Howard Hughes Medical Institute and Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06520, USA
    Cell 134:587-98. 2008
    ..These findings reveal a cell-intrinsic mechanism for initiation of autoimmunity, implicate the ISD pathway as the cause of AGS, and suggest an unanticipated contribution of endogenous retroelements to autoimmunity...
  17. ncbi YAP1 increases organ size and expands undifferentiated progenitor cells
    Fernando D Camargo
    Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, Massachusetts 02142, USA
    Curr Biol 17:2054-60. 2007
    ..Our data show that the Hippo signaling pathway regulates organ size in mammals and can act on stem cell compartments, indicating a potential link between stem/progenitor cells, organ size, and cancer...
  18. ncbi Zebrafish prickle, a modulator of noncanonical Wnt/Fz signaling, regulates gastrulation movements
    Michael T Veeman
    Department of Pharmacology, Center for Developmental Biology, Howard Hughes Medical Institute, University of Washington School of Medicine, Seattle, WA 98195, USA
    Curr Biol 13:680-5. 2003
    ..Our results support the idea that a vertebrate PCP pathway regulates gastrulation movements and suggest that there is overlap between the PCP and Wnt/calcium pathways...
  19. ncbi BAR domains as sensors of membrane curvature: the amphiphysin BAR structure
    Brian J Peter
    Medical Research Council MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Science 303:495-9. 2004
    ..From this, we predict that BAR domains are in many protein families, including sorting nexins, centaurins, and oligophrenins. The universal and minimal BAR domain is a dimerization, membrane-binding, and curvature-sensing module...
  20. pmc The role of YAP transcription coactivator in regulating stem cell self-renewal and differentiation
    Ian Lian
    Moores Cancer Center, University of California at San Diego, La Jolla, California 92093, USA
    Genes Dev 24:1106-18. 2010
    ..Moreover, YAP binds directly to promoters of a large number of genes known to be important for stem cells and stimulates their expression. Our observations establish a critical role of YAP in maintaining stem cell pluripotency...
  21. pmc The Hippo pathway target, YAP, promotes metastasis through its TEAD-interaction domain
    John M Lamar
    Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 109:E2441-50. 2012
    ..Together, our results suggest that increased YAP/TEAD activity plays a causal role in cancer progression and metastasis...
  22. pmc Yap1 acts downstream of α-catenin to control epidermal proliferation
    Karin Schlegelmilch
    Stem Cell Program, Children s Hospital, Boston, MA 02115, USA
    Cell 144:782-95. 2011
    ..Together, these data identify Yap1 as a determinant of the proliferative capacity of epidermal stem cells and as an important effector of a "crowd control" molecular circuitry in mammalian skin...
  23. ncbi Dishevelled: The hub of Wnt signaling
    Chan Gao
    Tsinghua University, Beijing, China
    Cell Signal 22:717-27. 2010
    ..In this review, we summarize the current understanding of Dvl functions in Wnt signal transduction and its biological functions in mouse development, and also discuss the molecular mechanisms of its actions...
  24. ncbi Wnt signaling and cancer
    P Polakis
    Department of Molecular Oncology, Genentech Inc, South San Francisco, California 94080 USA
    Genes Dev 14:1837-51. 2000
  25. ncbi Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions
    Viveka Mayya
    Department of Cell Biology, University of Connecticut Health Center, Farmington, 06030, USA
    Sci Signal 2:ra46. 2009
    ..We propose that modulation of PPIs by stimulus-dependent changes in S-T phosphorylation state is a widespread phenomenon applicable to many other signaling systems...
  26. pmc PHOSIDA (phosphorylation site database): management, structural and evolutionary investigation, and prediction of phosphosites
    Florian Gnad
    Department for Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Am Klopferspitz, D 82152 Martinsried, Germany
    Genome Biol 8:R250. 2007
    ..For each phosphosite, PHOSIDA lists matching kinase motifs, predicted secondary structures, conservation patterns, and its dynamic regulation upon stimulus. Using support vector machines, PHOSIDA also predicts phosphosites...
  27. ncbi Identification of nucleolin as a cellular receptor for human respiratory syncytial virus
    Farnoosh Tayyari
    The James Hogg Research Centre, Providence Heart and Lung Institute at St Paul s Hospital, Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada
    Nat Med 17:1132-5. 2011
    ..RNAi-mediated knockdown of lung nucleolin was associated with a significant reduction in RSV infection in mice (P = 0.0004), confirming that nucleolin is a functional RSV receptor in vivo...
  28. ncbi Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle
    Henrik Daub
    Cell Signaling Group, Department of Molecular Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Mol Cell 31:438-48. 2008
    ..These results provide a vastly extended knowledge base for functional studies on kinases and their regulation through site-specific phosphorylation...
  29. ncbi The substrate of Greatwall kinase, Arpp19, controls mitosis by inhibiting protein phosphatase 2A
    Aicha Gharbi-Ayachi
    Universités Montpellier 2 et 1, Centre de Recherche de Biochimie Macromoleculaire, CNRS UMR 5237, IFR 122, 1919 Route de Mende, 34293 Montpellier Cedex 5, France
    Science 330:1673-7. 2010
    ..Although both proteins can inhibit PP2A, endogenous Arpp19, but not α-Endosulfine, is responsible for PP2A inhibition at mitotic entry in Xenopus egg extracts...
  30. ncbi Greatwall phosphorylates an inhibitor of protein phosphatase 2A that is essential for mitosis
    Satoru Mochida
    Cancer Research UK, London Research Institute, Clare Hall Laboratories, South Mimms, Herts EN6 3LD, UK
    Science 330:1670-3. 2010
    ..This converts Ensa into a potent and specific inhibitor of PP2A-B55δ. This pathway represents a previously unknown element in the control of mitosis...
  31. pmc p63 is the molecular switch for initiation of an epithelial stratification program
    Maranke I Koster
    Program in Developmental Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Genes Dev 18:126-31. 2004
    ..Our data demonstrate that p63 plays a dual role: initiating epithelial stratification during development and maintaining proliferative potential of basal keratinocytes in mature epidermis...
  32. ncbi Quantitative phosphoproteomics of early elicitor signaling in Arabidopsis
    Joris J Benschop
    Molecular Genetics, Utrecht University, Padualaan 8, 3584CH Utrecht, The Netherlands
    Mol Cell Proteomics 6:1198-214. 2007
    ..Our study presents the largest quantitative Arabidopsis phosphoproteomics data set to date and provides a new resource that can be used to gain novel insight into plant defense signal transduction and early defense response...
  33. pmc Kibra functions as a tumor suppressor protein that regulates Hippo signaling in conjunction with Merlin and Expanded
    Jianzhong Yu
    Department of Biological Science, Florida State University, Tallahassee, FL 32306, USA
    Dev Cell 18:288-99. 2010
    ..These results shed light on the mechanism of Ex and Mer function and implicate Kibra as a potential tumor suppressor with relevance to neurofibromatosis...
  34. pmc PAT proteins, an ancient family of lipid droplet proteins that regulate cellular lipid stores
    Perry E Bickel
    Center for Diabetes and Obesity Research, Brown Foundation Institute of Molecular Medicine, University of Texas Health Science Center at Houston, Houston, Texas, USA
    Biochim Biophys Acta 1791:419-40. 2009
    ..In this review, we discuss how the PAT proteins regulate cellular lipid metabolism both in mammals and in model organisms...
  35. pmc Phosphoproteomic analysis reveals interconnected system-wide responses to perturbations of kinases and phosphatases in yeast
    Bernd Bodenmiller
    Institute of Molecular Systems Biology, ETH Zurich, 8093 Zurich, Switzerland
    Sci Signal 3:rs4. 2010
    ..cerevisiae) to determine the relationships between 97 kinases, 27 phosphatases, and more than 1000 phosphoproteins. We identified 8814 regulated phosphorylation events, describing the first system-wide protein ..
  36. pmc mTORC1-mediated cell proliferation, but not cell growth, controlled by the 4E-BPs
    Ryan J O Dowling
    Department of Biochemistry and Goodman Cancer Research Centre, McGill University, Montreal, Quebec H3A 1A3, Canada
    Science 328:1172-6. 2010
    ..Thus, control of cell size and cell cycle progression appear to be independent in mammalian cells, whereas in lower eukaryotes, 4E-BPs influence both cell growth and proliferation...
  37. ncbi PRAS40 is an insulin-regulated inhibitor of the mTORC1 protein kinase
    Yasemin Sancak
    Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, Nine Cambridge Center, Cambridge, MA 02142, USA
    Mol Cell 25:903-15. 2007
    ..We propose that the relative strengths of the rheb- and PRAS40-mediated inputs to mTORC1 set overall pathway activity and that insulin activates mTORC1 through the coordinated regulation of both...
  38. pmc Rho-kinase phosphorylates COOH-terminal threonines of ezrin/radixin/moesin (ERM) proteins and regulates their head-to-tail association
    T Matsui
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo ku, Kyoto 606, Japan
    J Cell Biol 140:647-57. 1998
  39. ncbi Insulin signalling to mTOR mediated by the Akt/PKB substrate PRAS40
    Emilie Vander Haar
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, 6 155 Jackson Hall, 321 Church Street SE, Minneapolis, MN 55455, USA
    Nat Cell Biol 9:316-23. 2007
    ..These findings identify PRAS40 as an important regulator of insulin sensitivity of the Akt-mTOR pathway and a potential target for the treatment of cancers, insulin resistance and hamartoma syndromes...
  40. ncbi ZO-1 and ZO-2 independently determine where claudins are polymerized in tight-junction strand formation
    Kazuaki Umeda
    Department of Cell Biology, Kyoto University Faculty of Medicine, Yoshida Konoe, Kyoto 606 8501, Japan
    Cell 126:741-54. 2006
    ..When NZO-1 was forcibly recruited to lateral membranes and dimerized, claudins were dramatically polymerized. These findings indicate that ZO-1 and ZO-2 can independently determine whether and where claudins are polymerized...
  41. ncbi Wnt induces LRP6 signalosomes and promotes dishevelled-dependent LRP6 phosphorylation
    Josipa Bilic
    Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
    Science 316:1619-22. 2007
    ..We propose that Wnts induce coclustering of receptors and Dvl in LRP6-signalosomes, which in turn triggers LRP6 phosphorylation to promote Axin recruitment and beta-catenin stabilization...
  42. pmc Absolute quantification of proteins and phosphoproteins from cell lysates by tandem MS
    Scott A Gerber
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 100:6940-5. 2003
    ..The methods described here represent focused, alternative approaches for studying the dynamically changing proteome...
  43. pmc TAp63 induces senescence and suppresses tumorigenesis in vivo
    Xuecui Guo
    Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Nat Cell Biol 11:1451-7. 2009
    ..The ability of TAp63 to trigger senescence and halt tumorigenesis irrespective of p53 status identifies TAp63 as a potential target of anti-cancer therapy for human malignancies with compromised p53...
  44. pmc A direct link between sister chromatid cohesion and chromosome condensation revealed through the analysis of MCD1 in S. cerevisiae
    V Guacci
    Howard Hughes Medical Institute, Carnegie Institution of Washington, Department of Embryology, Baltimore, Maryland 21210, USA
    Cell 91:47-57. 1997
    ..These data implicate Mcd1p as a nexus between cohesion and condensation. We present a model for mitotic chromosome structure that incorporates this previously unsuspected link...
  45. ncbi 53BP1, a mediator of the DNA damage checkpoint
    Bin Wang
    Verna and Mars McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Science 298:1435-8. 2002
  46. ncbi Insulin-dependent stimulation of protein synthesis by phosphorylation of a regulator of 5'-cap function
    A Pause
    Department of Biochemistry, McGill University, Montreal, Quebec, Canada
    Nature 371:762-7. 1994
    ..The action of this new regulator of protein synthesis is therefore modulated by insulin, which acts to stimulate the overall rate of translation and promote cell growth...
  47. ncbi Paxillin: adapting to change
    Michael C Brown
    Dept of Cell and Developmental Biology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210, USA
    Physiol Rev 84:1315-39. 2004
  48. pmc 4E-BP1 is a key effector of the oncogenic activation of the AKT and ERK signaling pathways that integrates their function in tumors
    Qing Bai She
    Program in Molecular Pharmacology and Chemistry, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
    Cancer Cell 18:39-51. 2010
    ..Thus, 4E-BP1 plays a prominent role in mediating the effects of these pathways in tumors in which they are activated by mutation...
  49. pmc A multidimensional chromatography technology for in-depth phosphoproteome analysis
    Claudio P Albuquerque
    Ludwig Institute for Cancer Research, Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093, USA
    Mol Cell Proteomics 7:1389-96. 2008
    ..Saccharomyces cerevisiae after DNA damage led to the identification of 8764 unique phosphopeptides from 2278 phosphoproteins using tandem MS...
  50. pmc RASSF1A elicits apoptosis through an MST2 pathway directing proapoptotic transcription by the p73 tumor suppressor protein
    David Matallanas
    Signaling and Proteomics Laboratory, The Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, UK
    Mol Cell 27:962-75. 2007
    ..Our results describe an MST2-dependent effector pathway for RASSF1A proapoptotic signaling and indicate that silencing of RASSF1A in tumors removes a proapoptotic signal emanating from p73...
  51. pmc A coordinated phosphorylation by Lats and CK1 regulates YAP stability through SCF(beta-TRCP)
    Bin Zhao
    Department of Pharmacology and Moores Cancer Center, University of California at San Diego, La Jolla, California 92093 0815, USA
    Genes Dev 24:72-85. 2010
  52. ncbi SIN1/MIP1 maintains rictor-mTOR complex integrity and regulates Akt phosphorylation and substrate specificity
    Estela Jacinto
    Department of Physiology and Biophysics, UMDNJ Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA
    Cell 127:125-37. 2006
    ..Our findings reveal that the SIN1-rictor-mTOR function in Akt-Ser473 phosphorylation is required for TORC2 function in cell survival but is dispensable for TORC1 function...
  53. pmc Adoption of PERILIPIN as a unifying nomenclature for the mammalian PAT-family of intracellular lipid storage droplet proteins
    Alan R Kimmel
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA
    J Lipid Res 51:468-71. 2010
    ..Each gene member will incorporate the root term PERILIPIN (PLIN), the founding gene of the PAT family, with the different genes/proteins numbered sequentially...
  54. ncbi Shugoshin collaborates with protein phosphatase 2A to protect cohesin
    Tomoya S Kitajima
    Laboratory of Chromosome Dynamics, Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan
    Nature 441:46-52. 2006
    ..Thus, we have revealed a conserved mechanism of centromeric protection of eukaryotic chromosomes in mitosis and meiosis...
  55. pmc A core function for p120-catenin in cadherin turnover
    Michael A Davis
    Department of Cancer Biology, Vanderbilt University, Nashville, TN 37232 6840, USA
    J Cell Biol 163:525-34. 2003
    ..The data reveal a core function of p120 in cadherin complexes, and strongly predict a dose-dependent loss of E-cadherin in tumors that partially or completely down-regulate p120...
  56. pmc Discovery and development of the G-rich oligonucleotide AS1411 as a novel treatment for cancer
    Paula J Bates
    James Graham Brown Cancer Center, Department of Medicine, University of Louisville, Louisville, Kentucky 40202, USA
    Exp Mol Pathol 86:151-64. 2009
    ..Thus, the serendipitous discovery of the G-rich oligonucleotides also led to the identification of nucleolin as a new molecular target for cancer therapy...
  57. ncbi Regulation of planar cell polarity by Smurf ubiquitin ligases
    Masahiro Narimatsu
    Center for Systems Biology, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, ON, Canada, M5G 1X5
    Cell 137:295-307. 2009
    ..Our work thus uncovers ubiquitin ligases in a mechanistic link between noncanonical Wnt signaling and PCP/CE...
  58. pmc Loss of DMP1 causes rickets and osteomalacia and identifies a role for osteocytes in mineral metabolism
    Jian Q Feng
    Oral Biology, University of Missouri Kansas City, Kansas City, Missouri 64108, USA
    Nat Genet 38:1310-5. 2006
    ..Our findings suggest a bone-renal axis that is central to guiding proper mineral metabolism...
  59. pmc Expression of Yes-associated protein in common solid tumors
    Angela A Steinhardt
    Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Hum Pathol 39:1582-9. 2008
  60. ncbi Postreplicative recruitment of cohesin to double-strand breaks is required for DNA repair
    Lena Ström
    Department of Cell and Molecular Biology, Karolinska Institute, Berzelius vag 35, 171 77 Stockholm, Sweden
    Mol Cell 16:1003-15. 2004
    ..Evidence is also provided that DSB induction elicits establishment of sister chromatid cohesion in G2, implicating that damage-recruited cohesin facilitates DNA repair by tethering chromatids...
  61. ncbi Tumor suppressor LATS1 is a negative regulator of oncogene YAP
    Yawei Hao
    Department of Pathology and Molecular Medicine, Queen s University, Kingston, Ontario K7L 3N6, Canada
    J Biol Chem 283:5496-509. 2008
    ..These research findings will have profound impacts on our understanding of the molecular mechanism of the LATS tumor suppressor and the emerging Hippo-LATS/Warts pathway...
  62. ncbi Disruption of IRS-2 causes type 2 diabetes in mice
    D J Withers
    Howard Hughes Medical Institute, Joslin Diabetes Center, Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215, USA
    Nature 391:900-4. 1998
    ..Our results indicate that dysfunction of IRS-2 may contribute to the pathophysiology of human type 2 diabetes...
  63. pmc Differential recruitment of Dishevelled provides signaling specificity in the planar cell polarity and Wingless signaling pathways
    J D Axelrod
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115 USA
    Genes Dev 12:2610-22. 1998
    ..The PCP signal may selectively result in focal Fz activation and asymmetric relocalization of Dsh to the membrane, where Dsh effects cytoskeletal reorganization to orient prehair initiation...
  64. ncbi Analytical strategies for phosphoproteomics
    Tine E Thingholm
    KMEB, Department of Endocrinology, Odense University Hospital, Odense M, Denmark
    Proteomics 9:1451-68. 2009
    ..Phosphorylation is a transient modification, and phosphoproteins are often very low abundant...
  65. ncbi Molecular architecture of SMC proteins and the yeast cohesin complex
    Christian H Haering
    Research Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030 Vienna, Austria
    Mol Cell 9:773-88. 2002
    ..The two heads of the V-shaped dimer are connected by different ends of the cleavable Scc1 subunit. Cohesin therefore forms a large proteinaceous loop within which sister chromatids might be entrapped after DNA replication...
  66. pmc Tumor suppressor p53 binding protein 1 (53BP1) is involved in DNA damage-signaling pathways
    I Rappold
    Division of Oncology Research, Mayo Clinic, Rochester, Minnesota 55905, USA
    J Cell Biol 153:613-20. 2001
    ..Third, 53BP1 is readily phosphorylated by ATM in vitro. Taken together, these results suggest that 53BP1 is an ATM substrate that is involved early in the DNA damage-signaling pathways in mammalian cells...
  67. ncbi Serine phosphorylation of insulin receptor substrate 1 by inhibitor kappa B kinase complex
    Zhanguo Gao
    Pennington Biomedical Research Center, Louisiana State University, Baton Rouge 70808, USA
    J Biol Chem 277:48115-21. 2002
  68. pmc Identification and characterization of nucleolin as a c-myc G-quadruplex-binding protein
    Veronica Gonzalez
    College of Pharmacy, University of Arizona, Tucson, Arizona 85721, USA
    J Biol Chem 284:23622-35. 2009
    ..Finally, we show that nucleolin binds to the c-myc promoter in HeLa cells, which indicates that this interaction occurs in vivo. In summary, nucleolin may induce c-myc G4 formation in vivo...
  69. pmc Maturation of active zone assembly by Drosophila Bruchpilot
    Wernher Fouquet
    Institute for Biology Genetics, Free University Berlin, Berlin, Germany
    J Cell Biol 186:129-45. 2009
    ..BRP seems responsible for effectively clustering Ca(2+) channels beneath the T-bar density late in a protracted AZ formation process, potentially through a direct molecular interaction with intracellular Ca(2+) channel domains...
  70. ncbi Regulation of p53 translation and induction after DNA damage by ribosomal protein L26 and nucleolin
    Masatoshi Takagi
    Department of Hematology Oncology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Cell 123:49-63. 2005
    ..These findings demonstrate the importance of increased translation of p53 in DNA-damage responses and suggest critical roles for RPL26 and nucleolin in affecting p53 induction...
  71. pmc Heterogeneous nuclear ribonucleoprotein K and nucleolin as transcriptional activators of the vascular endothelial growth factor promoter through interaction with secondary DNA structures
    Diana J Uribe
    Pharmacology and Toxicology Department, University of Arizona, Tucson, Arizona 85721, USA
    Biochemistry 50:3796-806. 2011
    ..Taken together, the identification of transcription factors that can recognize and bind to atypical DNA structures within the pPu/pPy tract will provide new insight into mechanisms of transcriptional regulation of the VEGF gene...
  72. pmc Ezrin self-association involves binding of an N-terminal domain to a normally masked C-terminal domain that includes the F-actin binding site
    R Gary
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853, USA
    Mol Biol Cell 6:1061-75. 1995
    ..The conformationally inaccessible C-terminal region included the F-actin binding site, suggesting that this activity is likewise regulated by masking...
  73. pmc Mst1 and Mst2 maintain hepatocyte quiescence and suppress hepatocellular carcinoma development through inactivation of the Yap1 oncogene
    Dawang Zhou
    Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA
    Cancer Cell 16:425-38. 2009
    ..Approximately 30% of human HCCs show low Yap1(Ser127) phosphorylation and a majority exhibit loss of cleaved, activated Mst1. Mst1/2 inhibition of Yap1 is an important pathway for tumor suppression in liver relevant to human HCC...
  74. pmc Dynamic interactions between 14-3-3 proteins and phosphoproteins regulate diverse cellular processes
    Carol MacKintosh
    MRC Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK
    Biochem J 381:329-42. 2004
  75. ncbi Mutations in the gene encoding the 3'-5' DNA exonuclease TREX1 cause Aicardi-Goutières syndrome at the AGS1 locus
    Yanick J Crow
    Leeds Institute of Molecular Medicine, University of Leeds, St James s University Hospital, Leeds LS9 7TF, UK
    Nat Genet 38:917-20. 2006
    ..Our findings suggest an unanticipated role for TREX1 in processing or clearing anomalous DNA structures, failure of which results in the triggering of an abnormal innate immune response...
  76. pmc Dissociation of cohesin from chromosome arms and loss of arm cohesion during early mitosis depends on phosphorylation of SA2
    Silke Hauf
    Research Institute of Molecular Pathology, Vienna, Austria
    PLoS Biol 3:e69. 2005
    ..The similarity of the phenotype obtained after expression of nonphosphorylatable SA2 in human cells to that seen after the depletion of Plk1 suggests that SA2 is the critical target of Plk1 in the cohesin dissociation pathway...
  77. pmc Cellular levels of p120 catenin function as a set point for cadherin expression levels in microvascular endothelial cells
    Kanyan Xiao
    Department of Dermatology, School of Medicine, Emory University, Atlanta, GA 30322, USA
    J Cell Biol 163:535-45. 2003
    ..These results demonstrate that cellular levels of p120ctn function as a set point mechanism that regulates cadherin expression levels, and that a major function of p120ctn is to control cadherin internalization and degradation...
  78. pmc A WW domain-containing yes-associated protein (YAP) is a novel transcriptional co-activator
    R Yagi
    Department of Viral Oncology, Institute for Virus Research, Kyoto University, Shogo in, Sakyo ku, Kyoto 606 8507, Japan
    EMBO J 18:2551-62. 1999
    ..These results indicate that the PY motif is a novel transcription activation domain that functions by recruiting YAP as a strong transcription activator to target genes...
  79. pmc PhosPhAt: the Arabidopsis thaliana phosphorylation site database. An update
    Pawel Durek
    Max Planck Institut fur Molekulare Pflanzenphysiologie, Am Muhlenberg 1, 14476 Golm, Germany
    Nucleic Acids Res 38:D828-34. 2010
    ..Finally, we have implemented functional annotation of proteins using MAPMAN ontology. These new developments make the PhosPhAt resource a useful and powerful tool for the scientific community as a whole beyond the plant sciences...
  80. ncbi Perp is a p63-regulated gene essential for epithelial integrity
    Rebecca A Ihrie
    Division of Radiation and Cancer Biology, Stanford University School of Medicine, Stanford, California 94305, USA
    Cell 120:843-56. 2005
    ..These findings demonstrate that Perp is a key effector in the p63 developmental program, playing an essential role in an adhesion subprogram central to epithelial integrity and homeostasis...
  81. ncbi Comparative analysis reveals conserved protein phosphorylation networks implicated in multiple diseases
    Chris Soon Heng Tan
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada M5G 1X5
    Sci Signal 2:ra39. 2009
    ..proteins tightly regulated by phosphorylation as well as signal integration hubs, and both types of phosphoproteins were enriched in proteins encoded by disease-associated genes...
  82. pmc Zonula occludens-1 and -2 are cytosolic scaffolds that regulate the assembly of cellular junctions
    Alan S Fanning
    The Department of Cell and Molecular Physiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7545, USA
    Ann N Y Acad Sci 1165:113-20. 2009
    ..Finally, we propose a model by which the functional activities of ZO proteins in the adherens junction and tight junction are differentiated by a novel regulatory motif known as the U6 or acidic motif...
  83. ncbi Disjunction of homologous chromosomes in meiosis I depends on proteolytic cleavage of the meiotic cohesin Rec8 by separin
    S B Buonomo
    Research Institute of Molecular Pathology, University of Vienna, Austria
    Cell 103:387-98. 2000
    ..We show here that cleavage of Rec8 by separin at one of two different sites is necessary for the resolution of chiasmata and the disjunction of homologous chromosomes during meiosis...
  84. ncbi Epsin is an EH-domain-binding protein implicated in clathrin-mediated endocytosis
    H Chen
    Howard Hughes Medical Institute and Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    Nature 394:793-7. 1998
    ..We propose that epsin may participate, together with Eps15, in the molecular rearrangement of the clathrin coats that are required for coated-pit invagination and vesicle fission...
  85. ncbi RIAM, an Ena/VASP and Profilin ligand, interacts with Rap1-GTP and mediates Rap1-induced adhesion
    Esther M Lafuente
    Department of Medical Oncology, Dana Farber Cancer Institute, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Dev Cell 7:585-95. 2004
    ..RIAM knockdown displaced Rap1-GTP from the plasma membrane and abrogated Rap1-induced adhesion. Thus, RIAM links Rap1 to integrin activation and plays a role in regulating actin dynamics...
  86. ncbi Yes-associated protein 1 exhibits oncogenic property in gastric cancer and its nuclear accumulation associates with poor prognosis
    Wei Kang
    Department of Anatomical and Cellular Pathology, Sir YK Pao Center for Cancer, State Key Laboratory in Oncology in South China, Institute of Digestive Disease and Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Hong Kong
    Clin Cancer Res 17:2130-9. 2011
    ..The role of YAP1 in tumorigenesis is unclear. We aimed to investigate the functional role of YAP1 in tumorigenesis of gastric cancer...
  87. pmc Crowdsourcing network inference: the DREAM predictive signaling network challenge
    Robert J Prill
    IBM Computational Biology Center, Yorktown Heights, NY, 10598, USA
    Sci Signal 4:mr7. 2011
    ..We conclude that crowdsourcing the construction of PSNs-that is, outsourcing the task to the interested community-may be an effective strategy for network inference...
  88. ncbi Focal adhesion kinase: in command and control of cell motility
    Satyajit K Mitra
    The Scripps Research Institute, Department of Immunology, IMM21 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Rev Mol Cell Biol 6:56-68. 2005
    ..Through multifaceted and diverse molecular connections, FAK can influence the cytoskeleton, structures of cell adhesion sites and membrane protrusions to regulate cell movement...
  89. ncbi Insulin signaling in health and disease
    Morris F White
    Howard Hughes Medical Institute, Joslin Diabetes Center, Harvard Medical School, 1 Joslin Place, Boston, MA 02215, USA
    Science 302:1710-1. 2003
    ..Drugs that stimulate IRS2 (insulin receptor substrate protein 2) synthesis or signaling might be a good starting point. This knowledge will lead to rational strategies that prevent or cure diabetes...
  90. pmc The Merlin/NF2 tumor suppressor functions through the YAP oncoprotein to regulate tissue homeostasis in mammals
    Nailing Zhang
    Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Dev Cell 19:27-38. 2010
    ..Our studies link Merlin/NF2 to mammalian Hippo signaling and implicate YAP activation as a mediator of pathologies relevant to Neurofibromatosis 2...
  91. pmc Cohesins form chromosomal cis-interactions at the developmentally regulated IFNG locus
    Suzana Hadjur
    Lymphocyte Development Group, MRC Clinical Sciences Centre, Imperial College London, Du Cane Road, London W12 0NN, UK
    Nature 460:410-3. 2009
    ..This new aspect of cohesin function is probably important for normal development and disease...
  92. pmc Phosphoinositide-3-OH kinase-dependent regulation of glycogen synthase kinase 3 and protein kinase B/AKT by the integrin-linked kinase
    M Delcommenne
    British Columbia Cancer Agency, Jack Bell Research Centre, 2660 Oak Street, Vancouver, British Columbia V6H 3Z6 Canada
    Proc Natl Acad Sci U S A 95:11211-6. 1998
    ..ILK is thus a receptor-proximal effector for the Pi(3)K-dependent, extracellular matrix and growth factor mediated, activation of PKB/AKT, and inhibition of GSK-3...
  93. pmc IFI16 acts as a nuclear pathogen sensor to induce the inflammasome in response to Kaposi Sarcoma-associated herpesvirus infection
    Nagaraj Kerur
    H M Bligh Cancer Research Laboratories, Department of Microbiology and Immunology, Chicago Medical School, Rosalind Franklin University of Medicine and Science, 3333 Green Bay Road, North Chicago, IL 60064, USA
    Cell Host Microbe 9:363-75. 2011
    ..Caspase-1 activation by KSHV was reduced by IFI16 and ASC silencing. Our studies reveal IFI16 as a nuclear pathogen sensor and demonstrate that the inflammasome also functions in the nucleus...
  94. pmc Phospho.ELM: a database of phosphorylation sites--update 2008
    Francesca Diella
    Structural and Computational Biology Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany
    Nucleic Acids Res 36:D240-4. 2008
    ..Phospho.ELM is available on line at:
  95. pmc TEAD/TEF transcription factors utilize the activation domain of YAP65, a Src/Yes-associated protein localized in the cytoplasm
    A Vassilev
    National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892 2753, USA
    Genes Dev 15:1229-41. 2001
    ..Because TEAD-dependent transcription was limited by YAP65, and YAP65 also binds Src/Yes protein tyrosine kinases, we propose that YAP65 regulates TEAD-dependent transcription in response to mitogenic signals...
  96. pmc A novel role for p120 catenin in E-cadherin function
    Renee C Ireton
    Department of Cancer Biology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    J Cell Biol 159:465-76. 2002
    ..These data reveal a cooperative interaction between p120 and E-cadherin and a novel role for p120 that is likely indispensable in normal cells...
  97. pmc Conversion of zonulae occludentes from tight to leaky strand type by introducing claudin-2 into Madin-Darby canine kidney I cells
    M Furuse
    Department of Cell Biology, Faculty of Medicine, Kyoto University, Kyoto 606 8501, Japan
    J Cell Biol 153:263-72. 2001
  98. ncbi Raptor, a binding partner of target of rapamycin (TOR), mediates TOR action
    Kenta Hara
    Biosignal Research Center, Kobe University, 657 8501, Kobe, Japan
    Cell 110:177-89. 2002
    ..elegans raptor yields an array of phenotypes that closely resemble those produced by inactivation of Ce-TOR. Thus, raptor is an essential scaffold for the mTOR-catalyzed phosphorylation of 4EBP1 and mediates TOR action in vivo...
  99. ncbi Mutations in VANGL1 associated with neural-tube defects
    Zoha Kibar
    Department of Biochemistry, McGill University, Montreal, QC, Canada
    N Engl J Med 356:1432-7. 2007
    ..In a protein-protein interaction assay V239I abolished interaction of VANGL1 protein with its binding partners, disheveled-1, -2, and -3. These findings implicate VANGL1 as a risk factor in human neural-tube defects...
  100. ncbi Intersectin, a novel adaptor protein with two Eps15 homology and five Src homology 3 domains
    M Yamabhai
    Department of Pharmacology, University of Wisconsin, Madison, Wisconsin 53706 1532, USA
    J Biol Chem 273:31401-7. 1998
    ..We propose that Intersectin is a component of the endocytic machinery...
  101. ncbi Smad regulation in TGF-beta signal transduction
    A Moustakas
    Ludwig Institute for Cancer Research, Box 595, SE 751 24 Uppsala, Sweden
    J Cell Sci 114:4359-69. 2001
    ..Finally, the inhibitory Smads (I-Smads) block phosphorylation of R-Smads by the receptors and promote ubiquitination and degradation of receptor complexes, thus inhibiting signalling...

Research Grants64

  1. Phosphorylation of FGF23 coordinates crosstalk between the skeleton and kidney
    ..The majority of phosphoproteins are intracellular;however, numerous extracellular proteins are also phosphorylated...
  2. Using Mass Spectroscopy to Characterize Transcription Proteins and F2-Isoprostane
    BETTYE HENNINTON; Fiscal Year: 2011
    ..The specific aims are: Specific Aim 1: To test the hypothesis that Rap1 p and Gcr1 p are phosphoproteins. Specific Aim 2: To test the hypothesis that phosphorylation is either increased or decreased on Gcrlp when ..
  3. Defensin-induced adaptive immunity to HagB
    Kim A Brogden; Fiscal Year: 2013
    ..Pro-inflammatory cytokines in supernatants and MAPK phosphoproteins in lysates of cells exposed to HBD3 and rHagB or rFimA mixtures will be determined with and without signal ..
  4. Myxoma Virus (MV) Oncolysis for treating human cancer
    Grant McFadden; Fiscal Year: 2013
    ..changes, we probed MV-infected KG1 cells with an array of antibodies to 46 different human signaling phosphoproteins, and observed that MV infection specifically induces Stat5 and Hck activation in KG1 cells...
  5. Implementation of phosphoprotein preservation technology for cancer biospecimens
    LANCE ALLEN LIOTTA; Fiscal Year: 2013
    ..development and clinical validation of an innovative and transformative technology for preserving tissue phosphoproteins and diagnostic histomorphology for clinical cancer molecular profiling...
  6. Varicella-Zoster Virus: T Cell/Skin Tropism &Immunity
    Ann Arvin; Fiscal Year: 2013
    ..applied to studies of human T cell biology;methods will include multiparameter flow cytometric analysis of phosphoproteins, multiplex cytokine/chemokine assays and microfluidics-based profiling of gene expression (Aim 1)...
  7. Analysis of Candida albicans filamentation using SILAC
    DEREK PAUL THOMAS; Fiscal Year: 2012
    ..a few specific proteins and there are limited proteome-wide data on signal transduction pathway proteins or phosphoproteins in general. This application will study the C...
    Victor H Engelhard; Fiscal Year: 2012
    ..The source proteins for the class I MHC-associated phosphopeptides identified to date are in large part known phosphoproteins. This is consistent with the hypothesis that these peptides are processed during the degradation of folded ..
  9. Phosphorylation-dependent regulation of epithelial sodium channel (ENaC) traffick
    Xiubin Liang; Fiscal Year: 2012
    ..Based on our prior findings, 14-3-3 proteins are essential stabilizers of the phosphoproteins that regulate ENaC trafficking...
  10. Cellular Immune Response to RVFV Infection
    KIMBERLY KERSHEA GRAY; Fiscal Year: 2010
    ..should result in a decrease of pro-inflammatory and Th1 cytokines and the increase of various pro-apoptotic phosphoproteins. With a better understanding of the host response to RVFV infection, we will be able to pursue intelligently ..
  11. Cellular/Molecular Analysis of Short &Long-Term Memory
    TERRY J CROW; Fiscal Year: 2013
    ..We will identify proteins in the 2D gel field of all 35S-labeled proteins that are phosphoproteins by incubating lysate samples of conditioned and control nervous systems with both 35S-methionine and 32P-..
  12. Regulation of M phase exit
    SALLY A KORNBLUTH; Fiscal Year: 2010
    ..Cdc2)/Cyclin B complexes, while M phase exit requires Cyclin B degradation and dephosphorylation of mitotic phosphoproteins. The destruction of Cyclin B at M phase exit is driven by the APC (anaphase promoting complex), a multi-..
  13. The effects of PTEN on tyrosine kinase signaling in breast cancer
    Todd W Miller; Fiscal Year: 2011
    ..and the effects of PTEN loss on tyrosine kinase signaling will identify novel roles of PTEN, offer candidate phosphoproteins altered by PTEN loss, and reveal new connections between signaling pathways...
  14. Enhanced proteomics capabilities for quantitation of proteins with a triple quad
    KENNETH DONALD GREIS; Fiscal Year: 2010
    ..studies have been hampered by the lack of a high sensitivity instrument capable of quantifying proteins and phosphoproteins with enough sensitivity and precision to measure relevant modulation of phosphorylation...
    Beerelli Seshi; Fiscal Year: 2011
    ..Facilitate understanding of the pathogenetic mechanisms by identifying the phosphoproteins potentially involved in cell signaling pathways...
  16. The effects of PTEN on tyrosine kinase signaling in breast cancer
    Todd W Miller; Fiscal Year: 2013
    ..and the effects of PTEN loss on tyrosine kinase signaling will identify novel roles of PTEN, offer candidate phosphoproteins altered by PTEN loss, and reveal new connections between signaling pathways...
  17. Novel Mass Spectrometer for Comprhensive No-Loss MS/MS of All Stored Ions
    Sunnie Myung; Fiscal Year: 2010 the low abundance of post-trans la tiona I ly modified proteins in complex biological mixtures such as phosphoproteins. This mandates a need for robust and highly sensitive detection system in which mass spectrometry (MS) has ..
  18. Bone Sialprotein in Osteogenesis and Bone Regeneration
    Jinxi Wang; Fiscal Year: 2013 applicant): Bone sialoprotein (BSP) is one of the major non-collagenous extracellular matrix (ECM) phosphoproteins in bone and dentin...
  19. Role of 14-3-3o in development and repair of corneal epithelium
    Qiutang Li; Fiscal Year: 2013
    ..14-3-3 is a family of proteins that bind phosphoproteins and regulate their subcellular localization...
  20. Intracellular and Extracellular function of dentin phosphophoryn
    Anne George; Fiscal Year: 2013
    ..Acidic phosphoproteins of the organic matrix of bone and dentin have been implicated as regulators of the nucleation process and ..
  21. Phosphoinositide Phosphatases
    Jack E Dixon; Fiscal Year: 2013
    ..laboratory has demonstrated that some phosphatases harboring CX5R motifs utilize phosphoinositides instead of phosphoproteins as their physiological substrates...
    Ronald Rubin; Fiscal Year: 1991
    ..14C-arachidonic acid, 32P) to label phospholipids, inositol polyphosphates, arachidonic acid metabolites, and phosphoproteins. We will investigate the relative abilities of phorbol esters and diacylglycerol analogues, which directly ..
  23. Physiology of Single Synaptic CNS Terminals
    TIMOTHY AIDAN RYAN; Fiscal Year: 2013
    ..Our goal is to specifically test the hypothesis that the abundant presynaptic phosphoproteins Synapsin and -catenin which are substrates of CDK5 are critical to mediating this control of these vesicle ..
  24. Phosphopeptide mapping of plexiform lesions in pulmonary arterial hypertension
    Lawrence S Zisman; Fiscal Year: 2012
    ..will be used to predict the kinases and/or kinase families most likely to be responsible for these increased phosphoproteins. The NetworKIN and other algorithms will be used for this purpose...
  25. Synthesis of phosphono-CheY and phosphono-VHR
    ..Protein tyrosine phosphatase enzymes break down phosphoproteins via the creation of a cysteinyl phosphate intermediate that is subsequently hydrolyzed...
  26. Ex Vivo Assay for In Situ Brain-Wide Mapping of Glutamate/GABA Metabolism
    Gerard Sanacora; Fiscal Year: 2013 characterize and compare the acute effects of glutamate modulating drugs with a broad range of potential therapeutic effects, on regional rates of glutamate/GABA neurotransmission with receptor activated signaling (phosphoproteins).
  27. Surrogate and Sentinal Technologies to Monitor Stability of Cancer Phosphoprotein
    Lance Liotta; Fiscal Year: 2009
    ..needle aspiration) for seamless collection, immediate preservation, and real time stability monitoring of phosphoproteins. Our multidisciplinary team has previously developed Laser Capture Microdissection (LCM) and reverse phase ..
  28. Endothelial Progenitor Cell Seeded Self-Expanding Vascular Stent
    HARDEAN ERIC ACHNECK; Fiscal Year: 2012
    ..physiological shear stress in an ex vivo flow circuit and test whether adhesion is mediated by cell wall phosphoproteins. In Aim I.C...
    Richard P Kraig; Fiscal Year: 2010
    ..This is due to complex interactive signaling at the level of ligands, receptors, and phosphoproteins. Further complexity stems from the diversity of brain cell types, with each capable of unique expression ..
  30. Phosphorylated signal pathway proteins: A new class of vitreous biomarkers for op
    Lance Liotta; Fiscal Year: 2009
    ..protein analyte panel in 20 microliters, and e) novel technology for affinity capture and enrichment of phosphoproteins in vitreous as a discovery tool for the future...
  31. Nanotechnology Enabled Top-Down Phosphoproteomics
    Song Jin; Fiscal Year: 2013
    ..Therefore, the effective capture, separation, and comprehensive analysis of phosphoproteins from complex biological samples are crucial for understanding fundamental cell biology and disease mechanisms ..
    Randy D Blakely; Fiscal Year: 2010
    ..Past studies have identified SERTs as phosphoproteins, regulated acutely (minutes) by multiple signaling pathways that influence transporter trafficking and ..
  33. Versatile scaffolds to visualize endogenous protein activation in living cells
    KLAUS MICHAEL HAHN; Fiscal Year: 2010
    ..protein modeling, variable regions will be introduced into naturally occurring domains already targeted to phosphoproteins, and protein scaffolds will be engineered for use as biosensors...
  34. Nanoparticle based phosphoprotein enrichment for the analysis of phosphoproteins
    CHAD JOHNATHAN DOOLEY; Fiscal Year: 2010
    ..phosphorylation by developing a class of multivalent nanoparticle materials capable of selectively enriching phosphoproteins out of a complex biological sample followed by top-down proteomic analysis of the enriched intact proteins...
  35. Greatwall Kinase and the Mitotic Control of Phosphatase Activity
    Michael L Goldberg; Fiscal Year: 2013
    ..kinase MPF (M phase promoting factor;Cdk1/cyclin B), which phosphorylates S/TP sites in a variety of mitotic phosphoproteins. Our laboratory has identified a novel kinase called Greatwall that is also required for the G2/M transition, ..
  36. GPCR Signaling in SCCHN: Integration with EGFR
    Jennifer Rubin Grandis; Fiscal Year: 2013 cetuximab resistance in 2 SCCHN patient cohorts and that GPCR targeting in combination with EGFR blockade modulates expression of proteins and phosphoproteins in human SCCHN tumors from subjects treated on a clinical protocol.
  37. Development of Ultrasound-facilitated Formalin-fixed and Paraffin-embedded Tissue
    ANDREW JI; Fiscal Year: 2011
    ..fixation can greatly improve the integrity of biomolecules in tissue specimens, especially for mRNA and phosphoproteins. The Phase I of this project is highly successful...
    Maria Bykhovskaia; Fiscal Year: 2010
    ..Synapsins, a family of neuron specific phosphoproteins, regulate binding of vesicles to actin filaments and thereby control availability of vesicles to become ..
  39. Chemical Approaches to Characterizing Kinase-catalyzed Modifications
    Mary Kay H Pflum; Fiscal Year: 2012
    ..Kinases phosphorylate protein substrates, making a characterization of phosphoproteins an important aspect of health-related research...
  40. Phosphoproteins in Insulin Signaling
    Gustav E Lienhard; Fiscal Year: 2011
    ..This knowledge is a necessary basis for understanding of the changes that occur in the insulin-resistant state of type 2 diabetes. ..
    W Waldman; Fiscal Year: 2004
    ..with formation of the viral tegument (our preliminary observations), several major components of which are phosphoproteins, molecular mechanisms underlying the antiviral activity of leflunomide will be investigated first by assay of ..
    Paul Greengard; Fiscal Year: 2013
    ..Project 3, "Striatal Phosphoproteins and the Actions of Psychostimulants," which will be a subcontract carried out at Yale University School of ..
    Richard Mitchell; Fiscal Year: 1999
    ..Therefore, proteins and phosphoproteins uniquely associated with wild-type mIg after internalization (vs...
    David Lebwohl; Fiscal Year: 1991
    ..Phosphotyrosine containing proteins are rare, constituting less than 0.2% of the phosphoproteins in cells...
    CHOU WU; Fiscal Year: 1993
    Phosphorylated matrix noncollagenous proteins (matrix phosphoproteins or MPP) have been proposed to play an important role in initiating the mineralization mechanism of bone and dentin...
    Mary Macdougall; Fiscal Year: 2000
    The goal of this project is to study the biological function of these dentin-specific phosphoproteins in the process of dentin biomineralization, thereby advancing our overall understanding of the process of odontoblast differentiation ..
    Paula Traktman; Fiscal Year: 2009
    ..proposal reflects our ongoing interest in the structure and function of the F10 kinase, and of various viral phosphoproteins which participate in the biogenesis of the virion membrane and the formation and maturation of the virion ..
    Ronald Victor; Fiscal Year: 2006
    ..pressure by stimulating renal sensory nerve endings containing synapsins, a family of vesicle-associated phosphoproteins that constitute putative substrates for calcineurin...
    John Leavitt; Fiscal Year: 1993
    ..Finally, plastin was discovered and identified as a family of calcium-binding phosphoproteins whose function is to bundle actin filaments at the edge of the cell...
    Paul Greengard; Fiscal Year: 2004
    ..application proposes a multi-disciplinary study of signal Project (Biochemical Analysis of Basal Ganglia Phosphoproteins) will include: biochemical characterization of the role of phosphorylation of Th475 and DARPP-32; elucidation ..
    Martin Surks; Fiscal Year: 1992
    ..We now propose to identify the specific proteins and phosphoproteins that are regulated by T3 at the Gl commitment point by pulse-labelling and 2-dimensional gel electrophoresis...
  52. Phosphoproteomics of Oral Fluids
    Erdjan Salih; Fiscal Year: 2009
    ..oral fluid since results of such work will most likely lead to the identification of novel unknown salivary phosphoproteins, and find direct applications in clinical dentistry...
    DONALD SENGER; Fiscal Year: 1993
    ..In situ hybridization with nucleic acid probes will provide for precise localization of expression of these two proteins...
  54. Novel soluble nanopolymers for enrichment of low abundant phosphoproteins
    Anton Iliuk; Fiscal Year: 2013
    ..In addition, high throughput formats for comprehensive phosphoproteomic analyses will be developed to address needs of many cancer biology and proteomics research labs/facilities. ..
    Bruce Liang; Fiscal Year: 1990
    The main objective is to investigate the role of cardiac phosphoproteins in the modulation of cardiac function and excitability...
    PHILIP MOBLEY; Fiscal Year: 1990
    ..will be on the mechanism by which protein kinase C regulates astrocyte morphology and the identification of phosphoproteins involved in this process...
    Paul Greengard; Fiscal Year: 2000 indicate that synapsins Ia and lb are highly homologous to another pair of synaptic vesicle-associated phosphoproteins, synapsins IIa and lIb, which we have also cloned and sequenced...
    Christopher Rhodes; Fiscal Year: 2005
    ..Surprisingly, the major beta-granule phosphoproteins are dephosphorylated in response to [Ca2+]i, implicating a novel role for PP-2B (a.k.a...
    Samuel Gandy; Fiscal Year: 1990
    ..Evidence has recently accumulated that a group of heretofore uncharacterized neuron-specific phosphoproteins is highly restricted to the basal ganglia...
    Gloria Lee; Fiscal Year: 1992
    ..Tau protein is a family of highly related phosphoproteins that promote in vitro assembly of microtubules and in vivo stabilization of microtubules...
    RICHARD LOLLEY; Fiscal Year: 1993
    This research program aims to investigate photoreceptor-specific phosphoproteins which complex with the beta-gamma subunits of transducin (T beta gamma) and which may participate in light- modulated activities of rod photoreceptors...
  62. itNETZ: Integrative and Translational Network-based Cellular Signature Analyzer
    Xiaobo Zhou; Fiscal Year: 2013
    ..for processing cellular images, Luminex genomics data, transcriptome sequencing data, and for modeling phosphoproteins signaling pathway;and second, data integration and mining toolkits for mapping genomics and proteomics to ..
    Khalil Ahmed; Fiscal Year: 1993
    ..1) To investigate the biochemistry of nuclear phosphoproteins and protein kinases (PKs) with the aim of defining their role in the androgen-mediated gene action in rat ..
    MARK IRELAND; Fiscal Year: 1990
    ..The development and aging aspects of lens cytoskeletal phosphoproteins will be examined in embryonic and post-hatching chicks and in epithelial cells induced to elongate...