molecular chaperones

Summary

Summary: A family of cellular proteins that mediate the correct assembly or disassembly of other polypeptides, and in some cases their assembly into oligomeric structures, but which are not components of those final structures. It is believed that chaperone proteins assist polypeptides to self-assemble by inhibiting alternative assembly pathways that produce nonfunctional structures. Some classes of molecular chaperones are the nucleoplasmins, the CHAPERONINS, the HEAT-SHOCK PROTEINS 70, and the HSP90 HEAT-SHOCK PROTEINS.

Top Publications

  1. ncbi HSP90 and the chaperoning of cancer
    Luke Whitesell
    Steele Memorial Children s Research Center, University of Arizona, Tucson, Arizona 85724, USA
    Nat Rev Cancer 5:761-72. 2005
  2. doi Molecular chaperones in protein folding and proteostasis
    F Ulrich Hartl
    Department of Cellular Biochemistry, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Nature 475:324-32. 2011
  3. doi HSP90 at the hub of protein homeostasis: emerging mechanistic insights
    Mikko Taipale
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nat Rev Mol Cell Biol 11:515-28. 2010
  4. ncbi Heat-shock proteins, molecular chaperones, and the stress response: evolutionary and ecological physiology
    M E Feder
    Department of Organismal Biology and Anatomy and Committee on Evolutionary Biology, University of Chicago, Illinois 60637, USA
    Annu Rev Physiol 61:243-82. 1999
  5. pmc One step at a time: endoplasmic reticulum-associated degradation
    Shruthi S Vembar
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    Nat Rev Mol Cell Biol 9:944-57. 2008
  6. ncbi Impaired degradation of mutant alpha-synuclein by chaperone-mediated autophagy
    Ana Maria Cuervo
    Department of Anatomy and Structural Biology, Marion Bessin Liver Research Center, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Science 305:1292-5. 2004
  7. ncbi Role of plant heat-shock proteins and molecular chaperones in the abiotic stress response
    Wangxia Wang
    The Robert H Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
    Trends Plant Sci 9:244-52. 2004
  8. ncbi Dynamic interaction of BiP and ER stress transducers in the unfolded-protein response
    A Bertolotti
    Skirball Institute of Biomolecular Medicine, Departments of Medicine and Cell Biology and the Kaplan Cancer Center, New York University School of Medicine, New York, New York 10016, USA
    Nat Cell Biol 2:326-32. 2000
  9. pmc Proteotoxic stress and inducible chaperone networks in neurodegenerative disease and aging
    Richard I Morimoto
    Department of Biochemistry, Molecular Biology, and Cell Biology, Rice Institute for Biomedical Research, Northwestern University, Evanston, Illinois 60208, USA
    Genes Dev 22:1427-38. 2008
  10. ncbi ER stress and diseases
    Hiderou Yoshida
    Department of Biophysics, Graduate School of Science, Kyoto University, Japan
    FEBS J 274:630-58. 2007

Detail Information

Publications273 found, 100 shown here

  1. ncbi HSP90 and the chaperoning of cancer
    Luke Whitesell
    Steele Memorial Children s Research Center, University of Arizona, Tucson, Arizona 85724, USA
    Nat Rev Cancer 5:761-72. 2005
    Standing watch over the proteome, molecular chaperones are an ancient and evolutionarily conserved class of proteins that guide the normal folding, intracellular disposition and proteolytic turnover of many of the key regulators of cell ..
  2. doi Molecular chaperones in protein folding and proteostasis
    F Ulrich Hartl
    Department of Cellular Biochemistry, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany
    Nature 475:324-32. 2011
    ..To avoid these dangers, cells invest in a complex network of molecular chaperones, which use ingenious mechanisms to prevent aggregation and promote efficient folding...
  3. doi HSP90 at the hub of protein homeostasis: emerging mechanistic insights
    Mikko Taipale
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nat Rev Mol Cell Biol 11:515-28. 2010
    ..Comprehensive understanding of how HSP90 functions promises not only to provide new avenues for therapeutic intervention, but to shed light on fundamental biological questions...
  4. ncbi Heat-shock proteins, molecular chaperones, and the stress response: evolutionary and ecological physiology
    M E Feder
    Department of Organismal Biology and Anatomy and Committee on Evolutionary Biology, University of Chicago, Illinois 60637, USA
    Annu Rev Physiol 61:243-82. 1999
    b>Molecular chaperones, including the heat-shock proteins (Hsps), are a ubiquitous feature of cells in which these proteins cope with stress-induced denaturation of other proteins...
  5. pmc One step at a time: endoplasmic reticulum-associated degradation
    Shruthi S Vembar
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    Nat Rev Mol Cell Biol 9:944-57. 2008
    ..During ERAD, molecular chaperones and associated factors recognize and target substrates for retrotranslocation to the cytoplasm, where they ..
  6. ncbi Impaired degradation of mutant alpha-synuclein by chaperone-mediated autophagy
    Ana Maria Cuervo
    Department of Anatomy and Structural Biology, Marion Bessin Liver Research Center, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Science 305:1292-5. 2004
    ..These findings may underlie the toxic gain-of-function by the mutants...
  7. ncbi Role of plant heat-shock proteins and molecular chaperones in the abiotic stress response
    Wangxia Wang
    The Robert H Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
    Trends Plant Sci 9:244-52. 2004
  8. ncbi Dynamic interaction of BiP and ER stress transducers in the unfolded-protein response
    A Bertolotti
    Skirball Institute of Biomolecular Medicine, Departments of Medicine and Cell Biology and the Kaplan Cancer Center, New York University School of Medicine, New York, New York 10016, USA
    Nat Cell Biol 2:326-32. 2000
    ..These findings are consistent with a model in which BiP represses signalling through PERK and IRE1 and protein misfolding relieves this repression by effecting the release of BiP from the PERK and IRE1 lumenal domains...
  9. pmc Proteotoxic stress and inducible chaperone networks in neurodegenerative disease and aging
    Richard I Morimoto
    Department of Biochemistry, Molecular Biology, and Cell Biology, Rice Institute for Biomedical Research, Northwestern University, Evanston, Illinois 60208, USA
    Genes Dev 22:1427-38. 2008
    ....
  10. ncbi ER stress and diseases
    Hiderou Yoshida
    Department of Biophysics, Graduate School of Science, Kyoto University, Japan
    FEBS J 274:630-58. 2007
    ..In this review, I will summarize recent progress in this field. Molecules that regulate the ER stress response would be potential candidates for drug targets in various conformational diseases...
  11. pmc Hsp70 chaperones: cellular functions and molecular mechanism
    M P Mayer
    Zentrum für Molekulare Biologie ZMBH, Universitat Heidelberg, Im Neuenheimer Feld 282, 69120, Heidelberg, Germany
    Cell Mol Life Sci 62:670-84. 2005
    Hsp70 proteins are central components of the cellular network of molecular chaperones and folding catalysts...
  12. doi Converging concepts of protein folding in vitro and in vivo
    F Ulrich Hartl
    Max Planck Institute of Biochemistry, Department of Cellular Biochemistry, Martinsried, Germany
    Nat Struct Mol Biol 16:574-81. 2009
    ..with a focus on how proteins navigate the complex folding energy landscape inside cells with the aid of molecular chaperones. Understanding these reactions is also of considerable medical relevance, as the aggregation of misfolding ..
  13. pmc ER chaperones in mammalian development and human diseases
    Min Ni
    Department of Biochemistry and Molecular Biology, USC Norris Comprehensive Cancer Center, Keck School of Medicine of the University of Southern California, 1441 Eastlake Ave, Los Angeles, CA 90089 9176, United States
    FEBS Lett 581:3641-51. 2007
    ....
  14. pmc Guidelines for the nomenclature of the human heat shock proteins
    Harm H Kampinga
    Department of Cell Biology, Section of Radiation and Stress Cell Biology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands
    Cell Stress Chaperones 14:105-11. 2009
    ..In addition to this nomenclature, we provide a list of the human Entrez Gene IDs and the corresponding Entrez Gene IDs for the mouse orthologs...
  15. ncbi S-nitrosylated protein-disulphide isomerase links protein misfolding to neurodegeneration
    Takashi Uehara
    Center for Neuroscience and Aging, Burnham Institute for Medical Research, 10901 North Torrey Pines Road, La Jolla, California 92037, USA
    Nature 441:513-7. 2006
    ..Thus, PDI prevents neurotoxicity associated with ER stress and protein misfolding, but NO blocks this protective effect in neurodegenerative disorders through the S-nitrosylation of PDI...
  16. doi The mitochondrial UPR - protecting organelle protein homeostasis
    Cole M Haynes
    Cell Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, Box 390, New York, NY 10065, USA
    J Cell Sci 123:3849-55. 2010
    ..Mitochondria have dedicated molecular chaperones and proteases that promote proper protein folding, complex assembly and quality control...
  17. pmc Wild type alpha-synuclein is degraded by chaperone-mediated autophagy and macroautophagy in neuronal cells
    Tereza Vogiatzi
    Division of Basic Neurosciences, Biomedical Research Foundation of the Academy of Athens, Soranou Efesiou 4, Athens, Greece
    J Biol Chem 283:23542-56. 2008
    ..These results indicate that CMA and macroautophagy are important pathways for WT ASYN degradation in neurons and underline the importance of CMA as degradation machinery in the nervous system...
  18. pmc CBP/p300-mediated acetylation of histone H3 on lysine 56
    Chandrima Das
    Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, PO Box 6511, Aurora Colorado 80045, USA
    Nature 459:113-7. 2009
    ....
  19. pmc Dopamine-modified alpha-synuclein blocks chaperone-mediated autophagy
    Marta Martinez-Vicente
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Yeshiva University, New York, New York 10461, USA
    J Clin Invest 118:777-88. 2008
    ..As blockage of CMA increases cellular vulnerability to stressors, we propose that dopamine-induced autophagic inhibition could explain the selective degeneration of PD dopaminergic neurons...
  20. ncbi GRP78 induction in cancer: therapeutic and prognostic implications
    Amy S Lee
    Department of Biochemistry and Molecular Biology, University of Southern California Norris Comprehensive Cancer Center, University of Southern California Keck School of Medicine, 1441 Eastlake Avenue, Los Angeles, CA 90089, USA
    Cancer Res 67:3496-9. 2007
    ..Furthermore, the recent discovery of GRP78 on the cell surface of cancer cells but not in normal tissues suggests that targeted therapy against cancer via surface GRP78 may be feasible...
  21. doi Biological and chemical approaches to diseases of proteostasis deficiency
    Evan T Powers
    Departments of Chemistry and Molecular and Experimental Medicine and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Annu Rev Biochem 78:959-91. 2009
    ..We propose that such therapeutic strategies, including combination therapies, represent a new approach for treating a range of diverse human maladies...
  22. doi Protein quality control in the cytosol and the endoplasmic reticulum: brothers in arms
    Alexander Buchberger
    Department of Biochemistry, Biocenter, University of Wurzburg, Am Hubland, 97074 Wurzburg, Germany
    Mol Cell 40:238-52. 2010
    ....
  23. ncbi Loss of the dystonia-associated protein torsinA selectively disrupts the neuronal nuclear envelope
    Rose E Goodchild
    Department of Neurology, Columbia University, New York, New York 10032, USA
    Neuron 48:923-32. 2005
    ..These observations demonstrate that neurons have a unique requirement for nuclear envelope localized torsinA function and suggest that loss of this activity is a key molecular event in the pathogenesis of DYT1 dystonia...
  24. ncbi Heat-shock protein 90, a chaperone for folding and regulation
    D Picard
    Cell Mol Life Sci 59:1640-8. 2002
    ..The large conformational flexibility of Hsp90 and a multitude of dynamic co-chaperone complexes contribute to generating functional diversity, and allow Hsp90 to assist a wide range of substrates...
  25. pmc Cellular strategies of protein quality control
    Bryan Chen
    Department of Biology and BioX Program, Stanford University, Stanford, California 94305, USA
    Cold Spring Harb Perspect Biol 3:a004374. 2011
    ..An elaborate network of molecular chaperones and protein degradation factors continually monitor and maintain the integrity of the proteome...
  26. ncbi RNA chaperones, RNA annealers and RNA helicases
    Lukas Rajkowitsch
    Max F Perutz Laboratories, University of Vienna, Vienna, Austria
    RNA Biol 4:118-30. 2007
    ..Finally, we present a new website for proteins with RNA chaperone activity which compiles all the information on these proteins with the perspective to promote the understanding of their activity...
  27. pmc BAG-6 is essential for selective elimination of defective proteasomal substrates
    Ryosuke Minami
    Department of Biological Sciences, Tokyo Metropolitan University, Tokyo, Japan
    J Cell Biol 190:637-50. 2010
    ..Therefore, we propose that BAG-6 is necessary for ubiquitin-mediated degradation of newly synthesized defective polypeptides...
  28. ncbi The Hsc70 co-chaperone CHIP targets immature CFTR for proteasomal degradation
    G C Meacham
    Department of Cell Biology and Cystic Fibrosis Center, University of Alabama Medical Center, 1918 University Boulevard, Birmingham, Alabama 35294 0005, USA
    Nat Cell Biol 3:100-5. 2001
    ..CHIP is a co-chaperone that converts Hsc70 from a protein-folding machine into a degradation factor that functions in endoplasmic-reticulum quality control...
  29. pmc A ribosome-associating factor chaperones tail-anchored membrane proteins
    Malaiyalam Mariappan
    Cell Biology and Metabolism Program, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nature 466:1120-4. 2010
    ..Thus, the Bat3 complex acts as a TMD-selective chaperone that effectively channels TA proteins to the TRC40 insertion pathway...
  30. ncbi The unfolded protein response: a stress signaling pathway critical for health and disease
    Kezhong Zhang
    Department of Biological Chemistry, Howard Hughes Medical Institute, University of Michigan Medical Center, Ann Arbor, MI 48109, USA
    Neurology 66:S102-9. 2006
    ..This review summarizes the complex regulation of UPR signaling and its relevance to human physiology and disease...
  31. ncbi Regulated translation initiation controls stress-induced gene expression in mammalian cells
    H P Harding
    Skirball Institute of Biomolecular Medicine The Department of Medicine, Kaplan Cancer Center New York University School of Medicine, New York, NY 10016, USA
    Mol Cell 6:1099-108. 2000
    ..Mammalian cells thus utilize an ancient pathway to regulate gene expression in response to diverse stress signals...
  32. ncbi Protein delivery into eukaryotic cells by type III secretion machines
    Jorge E Galan
    Section of Microbial Pathogenesis, Yale University School of Medicine, Boyer Center for Molecular Medicine, New Haven, Connecticut 0636, USA
    Nature 444:567-73. 2006
    ..The study of these systems is leading to unique insights into not only organelle assembly and protein secretion but also mechanisms of symbiosis and pathogenesis...
  33. ncbi A small ubiquitin-related polypeptide involved in targeting RanGAP1 to nuclear pore complex protein RanBP2
    R Mahajan
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Cell 88:97-107. 1997
    ..Inhibition of nuclear protein import resulting from antibodies directed at NPC-associated RanGAP1 cannot be overcome by soluble cytosolic RanGAP1, indicating that GTP hydrolysis by Ran at RanBP2 is required for nuclear protein import...
  34. ncbi Molecular chaperones and protein quality control
    Bernd Bukau
    Zentrum fur Molekulare Biologie, Universitat Heidelberg, 69120 Heidelberg, Germany
    Cell 125:443-51. 2006
    ..the removal of aggregates from the cytosol; reveal mechanisms for protein quality control in the endoplasmic reticulum; and provide new insight into two classes of molecular chaperones, the Hsp70 system and the AAA+ (Hsp100) unfoldases.
  35. ncbi Hsp90 cochaperone Aha1 downregulation rescues misfolding of CFTR in cystic fibrosis
    Xiaodong Wang
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Cell 127:803-15. 2006
    ..The activity of cargo-associated chaperome components may be a common mechanism regulating folding for ER exit, providing a general framework for correction of misfolding disease...
  36. doi Protein folding and modification in the mammalian endoplasmic reticulum
    Ineke Braakman
    Cellular Protein Chemistry, Faculty of Science, Utrecht University, Utrecht, The Netherlands
    Annu Rev Biochem 80:71-99. 2011
    ..How these various machineries interact during the normal pathway of folding and protein secretion is the subject of this review...
  37. pmc HIV integration targeting: a pathway involving Transportin-3 and the nuclear pore protein RanBP2
    Karen E Ocwieja
    Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA
    PLoS Pathog 7:e1001313. 2011
    ..Thus, our data support a model in which Gag-dependent engagement of the proper transport and nuclear pore machinery mediate trafficking of HIV complexes to sites of integration...
  38. pmc Hsp70 targets Hsp100 chaperones to substrates for protein disaggregation and prion fragmentation
    Juliane Winkler
    Center for Molecular Biology of the University of Heidelberg and German Cancer Research Center, DKFZ ZMBH Alliance, Universitat Heidelberg, Heidelberg D 69120, Germany
    J Cell Biol 198:387-404. 2012
    ..Our findings indicate a conserved mechanism of Hsp70-Hsp100 cooperation at the surface of protein aggregates and prion fibrils...
  39. ncbi Proapoptotic BAX and BAK modulate the unfolded protein response by a direct interaction with IRE1alpha
    Claudio Hetz
    Howard Hughes Medical Institute, Dana Farber Cancer Institute, and Harvard Medical School, Boston, MA 02115, USA
    Science 312:572-6. 2006
    ..Thus, BAX and BAK function at the ER membrane to activate IRE1alpha signaling and to provide a physical link between members of the core apoptotic pathway and the UPR...
  40. pmc SIRT1 protects against α-synuclein aggregation by activating molecular chaperones
    Gizem Donmez
    Paul F Glenn Laboratory and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Neurosci 32:124-32. 2012
    ..Thus, SIRT1 responds to α-synuclein aggregation-induced stress by activating molecular chaperones to protect against disease.
  41. ncbi Altered Hsp90 function in cancer: a unique therapeutic opportunity
    Rochelle Bagatell
    Department of Pediatrics and Hematology Oncology, Arizona Health Sciences Center, Room 5341, 1501 North Campbell Avenue, Tucson, AZ 85724, USA
    Mol Cancer Ther 3:1021-30. 2004
    b>Molecular chaperones or so-called heat shock proteins serve as central integrators of protein homeostasis within cells...
  42. pmc A molecular mechanism underlying the neural-specific defect in torsinA mutant mice
    Connie E Kim
    Department of Neurology and Integrated Graduate Program in Cellular, Molecular, Structural, and Genetic Studies, Columbia University, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 107:9861-6. 2010
    ....
  43. pmc Primary dystonia: molecules and mechanisms
    Lauren M Tanabe
    Department of Pharmacology, Columbia University, New York, NY, USA
    Nat Rev Neurol 5:598-609. 2009
    ..Other dystonia-related gene products traffic through the endoplasmic reticulum, suggesting a potential cell biological theme underlying primary dystonia...
  44. pmc Structural biology of the chaperone-usher pathway of pilus biogenesis
    Gabriel Waksman
    Institute of Structural and Molecular Biology, University College London and Birkbeck College, Malet Street, London, UK
    Nat Rev Microbiol 7:765-74. 2009
    ..Other studies have provided the molecular basis of host recognition by CU pili. The knowledge that has been gathered about both the assembly of and host recognition by CU pili has been harnessed to design promising antibiotic compounds...
  45. ncbi Heat shock response modulators as therapeutic tools for diseases of protein conformation
    Sandy D Westerheide
    Department of Biochemistry, Molecular Biology, and Cell Biology, Rice Institute for Biomedical Research, Northwestern University, Evanston, Illinois 60208, USA
    J Biol Chem 280:33097-100. 2005
    ..of components of the cellular quality control machinery, specifically the levels and activities of molecular chaperones, suppress aggregation and toxicity phenotypes to allow cellular function to be restored...
  46. ncbi Undetectable intracellular free copper: the requirement of a copper chaperone for superoxide dismutase
    T D Rae
    Department of Chemistry and Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208, USA
    Science 284:805-8. 1999
    ..These results indicate that intracellular [Cu]free is limited to less than one free copper ion per cell and suggest that a pool of free copper ions is not used in physiological activation of metalloenzymes...
  47. pmc Activation of the DNA damage checkpoint in yeast lacking the histone chaperone anti-silencing function 1
    Christopher Josh Ramey
    Department of Biochemistry and Molecular Genetics, University of Colorado Health Sciences Center at Fitzsimons, P O Box 6511, Aurora, CO 80045, USA
    Mol Cell Biol 24:10313-27. 2004
    ....
  48. ncbi The mammalian unfolded protein response
    Martin Schroder
    School of Biological and Biomedical Sciences, University of Durham, Durham DH1 3LE, United Kingdom
    Annu Rev Biochem 74:739-89. 2005
    ..Finally, we summarize data that demonstrate that UPR signaling feeds into decision making in other processes previously thought to be unrelated to ER function, e.g., eukaryotic starvation responses and differentiation programs...
  49. pmc Caught with their PAMPs down? The extracellular signalling actions of molecular chaperones are not due to microbial contaminants
    Brian Henderson
    UCL Eastman Dental Institute, University College London, UK
    Cell Stress Chaperones 15:123-41. 2010
    ..years, it has been hypothesised that a new signalling system may exist in vertebrates in which secreted molecular chaperones form a dynamic continuum between the cellular stress response and corresponding homeostatic physiological ..
  50. ncbi H-NS: a modulator of environmentally regulated gene expression
    T Atlung
    Department of Chemistry and Life Sciences, Roskilde University, Denmark
    Mol Microbiol 24:7-17. 1997
    ..Finally, we summarize the evolutionary and functional relationship between H-NS and the homologous StpA...
  51. doi HTRA proteases: regulated proteolysis in protein quality control
    Tim Clausen
    Research Institute of Molecular Pathology, Dr Bohrgasse 7, A 1030 Vienna, Austria
    Nat Rev Mol Cell Biol 12:152-62. 2011
    ....
  52. ncbi Compartment-specific perturbation of protein handling activates genes encoding mitochondrial chaperones
    Takunari Yoneda
    Skirball Institute of Biomolecular Medicine, Department of Cell Biology, New York University School of Medicine, 540 First Avenue, New York, NY 10016, USA
    J Cell Sci 117:4055-66. 2004
    ..These observations support the existence of a mitochondrial unfolded protein response that couples mitochondrial chaperone gene expression to changes in the protein handling environment in the organelle...
  53. ncbi Regulation of signaling protein function and trafficking by the hsp90/hsp70-based chaperone machinery
    William B Pratt
    Department of Pharmacology, University of Michigan Medical School, Ann Arbor, Michigan 48109 0632, USA
    Exp Biol Med (Maywood) 228:111-33. 2003
    ..This purified system should facilitate understanding of how eukaryotic hsp70 and hsp90 work together as essential components of a process that alters the conformations of substrate proteins to states that respond in signal transduction...
  54. pmc Nucleic acid chaperone activity of the ORF1 protein from the mouse LINE-1 retrotransposon
    S L Martin
    Department of Cellular and Structural Biology, University of Colorado School of Medicine, Denver, Colorado 80262, USA
    Mol Cell Biol 21:467-75. 2001
    ..These findings suggest a role for L1 ORF1p in mediating nucleic acid strand transfer steps during L1 reverse transcription...
  55. pmc Ran-dependent docking of importin-beta to RanBP2/Nup358 filaments is essential for protein import and cell viability
    Masakazu Hamada
    Department of Biochemistry and Molecular Biology and 2 Department of Pediatric and Adolescent Medicine, Mayo Clinic, Rochester, MN 55905, USA
    J Cell Biol 194:597-612. 2011
    ..These data suggest that a critical function of RanBP2 is to capture recycling RanGTP-importin-β complexes at cytoplasmic fibrils to allow for adequate cNLS-mediated cargo import...
  56. pmc Structural analysis of a monomeric form of the twin-arginine leader peptide binding chaperone Escherichia coli DmsD
    Charles M Stevens
    Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada
    J Mol Biol 389:124-33. 2009
    ..coli DmsA and the most conserved regions on the surface of EcDmsD...
  57. doi Ero1α regulates Ca(2+) fluxes at the endoplasmic reticulum-mitochondria interface (MAM)
    Tiziana Anelli
    Universita Vita Salute San Raffaele, Milano, Italy
    Antioxid Redox Signal 16:1077-87. 2012
    ..How these multiple functions are integrated with one another in living cells remains unclear...
  58. pmc An interdomain sector mediating allostery in Hsp70 molecular chaperones
    Robert G Smock
    Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, MA 01003, USA
    Mol Syst Biol 6:414. 2010
    Allosteric coupling between protein domains is fundamental to many cellular processes. For example, Hsp70 molecular chaperones use ATP binding by their actin-like N-terminal ATPase domain to control substrate interactions in their C-..
  59. pmc Structure and mechanism of protein stability sensors: chaperone activity of small heat shock proteins
    Hassane S McHaourab
    Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee 37232 0615, USA
    Biochemistry 48:3828-37. 2009
    Small heat shock proteins (sHSP) make up a remarkably diverse group of molecular chaperones possessing a degree of structural plasticity unparalleled in other protein superfamilies...
  60. ncbi Human leukocyte antigen-B-associated transcript 3 is released from tumor cells and engages the NKp30 receptor on natural killer cells
    Elke Pogge von Strandmann
    Department of Internal Medicine I, University Hospital of Cologne, Kerpener Str 62, D 50924 Cologne, Germany
    Immunity 27:965-74. 2007
    ..These data identify BAT3 as a cellular ligand for NKp30. We propose a concept for target cell recognition by NK cells beyond "missing self" and "induced self," mediated through a tumor cell-derived extracellular factor...
  61. pmc AAA-ATPase p97/Cdc48p, a cytosolic chaperone required for endoplasmic reticulum-associated protein degradation
    Efrat Rabinovich
    Department of Biochemistry, George S Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
    Mol Cell Biol 22:626-34. 2002
    ..responsible for import of nascent proteins, this bidirectional passage should be coordinated, probably by molecular chaperones. Here we implicate the cytosolic chaperone AAA-ATPase p97/Cdc48p in ERAD...
  62. ncbi Altered responses to dopaminergic D2 receptor activation and N-type calcium currents in striatal cholinergic interneurons in a mouse model of DYT1 dystonia
    A Pisani
    Clinica Neurologica, Dipartimento di Neuroscienze, Universita Tor Vergata, Rome, Italy, and Massachusetts General Hospital, Harvard Medical School, Boston 02114, USA
    Neurobiol Dis 24:318-25. 2006
    ..Our data support the existence of an imbalance between striatal dopaminergic and cholinergic signaling in DYT1 dystonia...
  63. ncbi Hsp27 (HspB1) and alphaB-crystallin (HspB5) as therapeutic targets
    André Patrick Arrigo
    Laboratoire Stress, Chaperons et Mort Cellulaire, CNRS, UMR5534, Centre de Genetique Moleculaire et Cellulaire, Universite Lyon 1, Bat Gregor Mendel, 16 rue Dubois, F 69622, Villeurbanne Cedex, France
    FEBS Lett 581:3665-74. 2007
    Hsp27 and alphaB-crystallin are molecular chaperones that are constitutively expressed in several mammalian cells, particularly in pathological conditions...
  64. doi Structural basis for the regulated protease and chaperone function of DegP
    Tobias Krojer
    Research Institute for Molecular Pathology IMP, Dr Bohrgasse 7, A 1030 Vienna, Austria
    Nature 453:885-90. 2008
    ..Oligomer reassembly and concomitant activation on substrate binding may also be critical in regulating other HtrA proteases implicated in protein-folding diseases...
  65. pmc Bat3 promotes the membrane integration of tail-anchored proteins
    Pawel Leznicki
    Faculty of Life Sciences, University of Manchester, Oxford Road, Manchester, M13 9PT, UK
    J Cell Sci 123:2170-8. 2010
    ....
  66. pmc A C-terminal translocation signal required for Dot/Icm-dependent delivery of the Legionella RalF protein to host cells
    Hiroki Nagai
    Section of Microbial Pathogenesis, Yale University School of Medicine, Boyer Center for Molecular Medicine, Room 354b, 295 Congress Avenue, New Haven, CT 06511, USA
    Proc Natl Acad Sci U S A 102:826-31. 2005
    ..Thus, Legionella has the ability to engage synthesized substrate proteins and transfer them into host cells on contact, enabling Legionella to rapidly alter transport of the vacuole in which it resides...
  67. pmc Chemical and biological approaches synergize to ameliorate protein-folding diseases
    Ting Wei Mu
    Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Cell 134:769-81. 2008
    ..It may be possible to ameliorate loss-of-function diseases by using proteostasis regulators alone or in combination with a pharmacologic chaperone...
  68. pmc Protein targeting and degradation are coupled for elimination of mislocalized proteins
    Tara Hessa
    Cell Biology and Metabolism Program, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nature 475:394-7. 2011
    ..We propose that such coupling allows the fast tracking of MLPs for degradation without futile engagement of the cytosolic folding machinery...
  69. pmc Perturbation of host nuclear membrane component RanBP2 impairs the nuclear import of human immunodeficiency virus -1 preintegration complex (DNA)
    Ruonan Zhang
    Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, Columbia, South Carolina, United States of America
    PLoS ONE 5:e15620. 2010
    ..RanBP2 could be a potential target for efficient inhibition of HIV...
  70. pmc HLA-B-associated transcript 3 (Bat3)/Scythe is essential for p300-mediated acetylation of p53
    Toru Sasaki
    The Campbell Family Institute for Breast Cancer Research CFIBCR, Ontario Cancer Institute OCI, University Health Network, Toronto, Ontario M5G 2C1, Canada
    Genes Dev 21:848-61. 2007
    ..Our data indicate that Bat3 is a novel and essential regulator of p53-mediated responses to genotoxic stress, and that Bat3 controls DNA damage-induced acetylation of p53...
  71. ncbi Heat shock proteins in cancer: chaperones of tumorigenesis
    Stuart K Calderwood
    Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
    Trends Biochem Sci 31:164-72. 2006
    ....
  72. pmc Cell cycle- and chaperone-mediated regulation of H3K56ac incorporation in yeast
    Tommy Kaplan
    School of Computer Science and Engineering, The Hebrew University, Jerusalem, Israel
    PLoS Genet 4:e1000270. 2008
    ....
  73. pmc Resolution of sister centromeres requires RanBP2-mediated SUMOylation of topoisomerase IIalpha
    Meelad M Dawlaty
    Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    Cell 133:103-15. 2008
    ..Furthermore, mice with low amounts of RanBP2 are highly sensitive to tumor formation. Together, these data identify RanBP2 as a chromosomal instability gene that regulates Topo IIalpha by sumoylation and suppresses tumorigenesis...
  74. ncbi Human Asf1 regulates the flow of S phase histones during replicational stress
    Anja Groth
    Institute of Cancer Biology, The Danish Cancer Society, Strandboulevarden 49, DK 2100 Copenhagen, Denmark
    Mol Cell 17:301-11. 2005
    ....
  75. ncbi The early-onset torsion dystonia gene (DYT1) encodes an ATP-binding protein
    L J Ozelius
    Molecular Neurogenetics Unit, Massachusetts General Hospital, Boston, USA
    Nat Genet 17:40-8. 1997
    ..This protein has homologues in nematode, rat, mouse and humans, with some resemblance to the family of heat-shock proteins and Clp proteases...
  76. ncbi Genome-wide replication-independent histone H3 exchange occurs predominantly at promoters and implicates H3 K56 acetylation and Asf1
    Anne Rufiange
    Centre de recherche en cancérologie de l Université Laval, L Hôtel Dieu de Québec CHUQ, Quebec, Canada
    Mol Cell 27:393-405. 2007
    ..Taken together, our data underline the dynamic nature of replication-independent nucleosome assembly/disassembly, specify a link to transcription, and implicate Asf1 and H3 K56 acetylation...
  77. ncbi Asf1 mediates histone eviction and deposition during elongation by RNA polymerase II
    Marc A Schwabish
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell 22:415-22. 2006
    ..Lastly, Asf1 inhibits internal initiation from cryptic promoters within coding regions. These results strongly suggest that Asf1 functions as an elongation factor to disassemble and reassemble histones during Pol II elongation...
  78. ncbi The copper chaperone for superoxide dismutase
    V C Culotta
    Division of Toxicological Sciences, Department of Environmental Health Sciences, Johns Hopkins University School of Public Health, Baltimore, Maryland 21205, USA
    J Biol Chem 272:23469-72. 1997
    ..Elucidation of the CCS copper delivery pathway may permit development of novel therapeutic approaches to human diseases that involve SOD1, including amyotrophic lateral sclerosis...
  79. ncbi ClpP mediates activation of a mitochondrial unfolded protein response in C. elegans
    Cole M Haynes
    Kimmel Center for Biology and Medicine of Skirball Institute and Department of Cell Biology, New York University School of Medicine, New York, NY 10016, USA
    Dev Cell 13:467-80. 2007
    ..As ClpP is known to function in the bacterial heat-shock response, our findings suggest that eukaryotes utilize component(s) from the protomitochondrial symbiont to signal the UPR(mt)...
  80. ncbi Chaperone properties of bacterial elongation factor EF-Tu
    T D Caldas
    Biochimie Génétique, Institut Jacques Monod, Universite Paris 7, 2 place Jussieu, 75005 Paris, France
    J Biol Chem 273:11478-82. 1998
    ..We report herewith that the Escherichia coli EF-Tu interacts with unfolded and denatured proteins as do molecular chaperones that are involved in protein folding and protein renaturation after stress...
  81. pmc Growth phase-dependent variation in protein composition of the Escherichia coli nucleoid
    T Ali Azam
    Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411 8540, Japan
    J Bacteriol 181:6361-70. 1999
    ..These changes in the composition of nucleoid-associated proteins in the stationary phase are accompanied by compaction of the genome DNA and silencing of the genome functions...
  82. ncbi Polyglutamine protein aggregates are dynamic
    Soojin Kim
    Department of Biochemistry, Molecular Biology and Cell Biology, Rice Institute for Biomedical Research, Northwestern University, Evanston, IL 60208, USA
    Nat Cell Biol 4:826-31. 2002
    ..These studies provide new insights into the composite organization and formation of protein aggregates and show that molecular chaperones are not sequestered into aggregates, but are instead transiently associated.
  83. pmc Quaternary dynamics and plasticity underlie small heat shock protein chaperone function
    Florian Stengel
    Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, United Kingdom
    Proc Natl Acad Sci U S A 107:2007-12. 2010
    Small Heat Shock Proteins (sHSPs) are a diverse family of molecular chaperones that prevent protein aggregation by binding clients destabilized during cellular stress...
  84. pmc Huntington toxicity in yeast model depends on polyglutamine aggregation mediated by a prion-like protein Rnq1
    Anatoli B Meriin
    Boston University School of Medicine, Boston, MA 02118, USA
    J Cell Biol 157:997-1004. 2002
    ..Our data indicated that deficiencies in molecular chaperones Sis1 and Hsp104 inhibited seeding of polyQ aggregates, whereas ssa1, ssa2, and ydj1-151 mutations inhibited ..
  85. pmc Histone H3-K56 acetylation is catalyzed by histone chaperone-dependent complexes
    Toshiaki Tsubota
    Program in Gene Function and Expression, University of Massachusetts Medical School, 364 Plantation Street 506, Worcester, MA 01605, USA
    Mol Cell 25:703-12. 2007
    ..Additionally, these complexes are functionally distinct, with the Rtt109/Asf1 complex, but not the Rtt109/Vps75 complex, being critical for resistance to genotoxic agents...
  86. ncbi Regulation of replication fork progression through histone supply and demand
    Anja Groth
    Laboratory of Nuclear Dynamics and Genome Plasticity, UMR218 CNRS Institut Curie, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    Science 318:1928-31. 2007
    ....
  87. pmc Histone chaperone Asf1 is required for histone H3 lysine 56 acetylation, a modification associated with S phase in mitosis and meiosis
    J Recht
    Laboratory of Chromatin Biology, The Rockefeller University, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 103:6988-93. 2006
    ..In contrast, multiple asf1 mutants that are resistant to DNA damage display WT levels of K56ac. These data suggest that maintenance of H3 K56 acetylation is a primary contribution of Asf1 to genome stability in yeast...
  88. ncbi Crystal structure of DegP (HtrA) reveals a new protease-chaperone machine
    Tobias Krojer
    Max Planck Institut fur Biochemie, Abteilung Strukturforschung, Am Klopferspitz 18a, 82152 Martinsried, Germany
    Nature 416:455-9. 2002
    b>Molecular chaperones and proteases monitor the folded state of other proteins...
  89. ncbi A common structural motif in the binding of virulence factors to bacterial secretion chaperones
    Mirjana Lilic
    Laboratory of Structural Microbiology, The Rockefeller University, New York, New York 10021, USA
    Mol Cell 21:653-64. 2006
    ..Disruption of this structural motif leads to a destabilization of several chaperone-substrate complexes from different species, as well as an impairment of secretion in Salmonella...
  90. pmc Huntingtin and the molecular pathogenesis of Huntington's disease. Fourth in molecular medicine review series
    Christian Landles
    Neurogenetics Laboratory, Medical and Molecular Genetics, GKT School of Medicine, King s College London, 8th Floor Guy s Tower, Guy s Hospital, London SE1 9RT, UK
    EMBO Rep 5:958-63. 2004
    ..We then focus on evidence that supports a role for transcriptional dysfunction and impaired protein folding and degradation as early events in disease pathogenesis...
  91. pmc Function of dopamine transporter is compromised in DYT1 transgenic animal model in vivo
    Jeff Hewett
    Department of Neurology, Massachusetts General Hospital, Boston, Massachusetts 02129, USA
    J Neurochem 113:228-35. 2010
    ..These data implies that altered DAT function may contribute to impaired DA neurotransmission and clinical symptoms in human DYT1 dystonia...
  92. pmc Understanding the functional interplay between mammalian mitochondrial Hsp70 chaperone machine components
    Arvind Vittal Goswami
    Department of Biochemistry, Indian Institute of Science, Bangalore 560012, Karnataka, India
    J Biol Chem 285:19472-82. 2010
    ..Additionally, we highlight the biochemical defects of the mtHsp70 mutant (G489E) associated with a myelodysplastic syndrome...
  93. pmc Chaperone control of the activity and specificity of the histone H3 acetyltransferase Rtt109
    Jeffrey Fillingham
    Banting and Best Department of Medical Research, Terrence Donnelly Centre for Cellular and Biomolecular Research CCBR, University of Toronto, 160 College St, Toronto, Ontario M5S 3E1, Canada
    Mol Cell Biol 28:4342-53. 2008
    ..Asf1 also physically interacts with the nuclear Hat1/Hat2/Hif1 complex that acetylates H4-K5 and H4-K12. We suggest Asf1 is capable of assembling into chromatin H3-H4 dimers diacetylated on both H4-K5/12 and H3-K9/56...
  94. pmc Cerebellothalamocortical connectivity regulates penetrance in dystonia
    Miklos Argyelan
    Center for Neurosciences, The Feinstein Institute for Medical Research, North Shore Long Island Jewish Health System, Manhasset, New York 11030, USA
    J Neurosci 29:9740-7. 2009
    ..Overall, these findings point to a novel mechanism to explain differences in clinical expression in carriers of genes for brain disease...
  95. doi Commentary: Dopaminergic dysfunction in DYT1 dystonia
    Thomas Wichmann
    Department of Neurology School of Medicine and Yerkes National Primate Research Center, Emory University, 954 Gatewood Road NE, Atlanta, GA 30329, USA
    Exp Neurol 212:242-6. 2008
    ....
  96. pmc Impaired striatal D2 receptor function leads to enhanced GABA transmission in a mouse model of DYT1 dystonia
    Giuseppe Sciamanna
    Department of Neuroscience, University Tor Vergata, Via Montpellier 1, 00133 Rome, Italy
    Neurobiol Dis 34:133-45. 2009
    ..Our findings demonstrate a disinhibition of striatal GABAergic synaptic activity, that can be at least partially attributed to a D2 DA receptor dysfunction...
  97. pmc The shock of aging: molecular chaperones and the heat shock response in longevity and aging--a mini-review
    Stuart K Calderwood
    Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
    Gerontology 55:550-8. 2009
    ..However, the precise mechanisms that determine the rates of aging in organisms are not known...
  98. pmc Cystamine and cysteamine increase brain levels of BDNF in Huntington disease via HSJ1b and transglutaminase
    Maria Borrell-Pages
    Institut Curie, CNRS UMR 146, Orsay, France
    J Clin Invest 116:1410-24. 2006
    ..Finally, cysteamine increases serum levels of BDNF in mouse and primate models of HD. Therefore, cysteamine is a potential treatment for HD, and serum BDNF levels can be used as a biomarker for drug efficacy...
  99. pmc Acetylation of histone H3 lysine 56 regulates replication-coupled nucleosome assembly
    Qing Li
    Department of Biochemistry and Molecular Biology, Mayo Clinic, College of Medicine, 200 First Street SW, Rochester, MN 55905, USA
    Cell 134:244-55. 2008
    ..These results reveal a mechanism by which H3K56Ac regulates replication-coupled nucleosome assembly mediated by CAF-1 and Rtt106...
  100. pmc Asf1 can promote trimethylation of H3 K36 by Set2
    Ling Ju Lin
    Department of Biochemistry, University of Alberta, MSB 5 76, Edmonton, Alberta, Canada T6G 2H7
    Mol Cell Biol 30:1116-29. 2010
    ..This function of Asf1 promotes the switch from di- to trimethylation of H3 K36 at that gene. These results support the view that Set2 function in chromatin metabolism can intimately involve histone chaperone Asf1...
  101. ncbi More than folding: localized functions of cytosolic chaperones
    Jason C Young
    Cellular Biochemistry, Max Planck Institute of Biochemistry, Am Klopferspitz 18a, D 82152, Martinsried, Germany
    Trends Biochem Sci 28:541-7. 2003
    ..Such flexibility is unique to the cytosolic Hsp70 and Hsp90 chaperone system...

Research Grants83

  1. Role of heat shock protein 90 in alcoholic liver disease
    Pranoti Mandrekar; Fiscal Year: 2010
    ..Heat shock proteins are induced by oxidative stress and function as molecular chaperones. Heat shock protein 90 (hsp90) and Grp94/gp96 chaperone signaling molecules of the TLR4 pathway to regulate ..
  2. MODIFICATION OF ALPHA-CRYSTALLIN CHAPERONE FUNCTION
    EDATHARA ABRAHAM; Fiscal Year: 2003
    ..We will test the hypothesis that posttranslational modifications affect the chaperone-target protein binding leading to reduced chaperone function. ..
  3. Role of Heat Shock Transcription Factor Hsf1 in Tumorigenesis
    Nahid F Mivechi; Fiscal Year: 2010
    ..hallmark in the pathogenesis of cancer is the increased expression of heat shock proteins (Hsps) and other molecular chaperones. This has been observed in many human tumor types, and is considered to be an adaptive response to enhance ..
  4. Role of Heat Shock Transcription Factor Hsf1 in Tumorigenesis
    NAHID MIVECHI; Fiscal Year: 2009
    ..hallmark in the pathogenesis of cancer is the increased expression of heat shock proteins (Hsps) and other molecular chaperones. This has been observed in many human tumor types, and is considered to be an adaptive response to enhance ..
  5. Genetic Basis of Non-Familial IgA Nephropathy
    Tongzhong Ju; Fiscal Year: 2010
    ....
  6. Genetic Basis of Non-Familial IgA Nephropathy
    Tongzhong Ju; Fiscal Year: 2009
    ....
  7. Celestrols for Treatment of Neurodegenerative Diseases
    Richard Silverman; Fiscal Year: 2007
    The expression of molecular chaperones has been shown to suppress protein misfolding/aggregation and cellular toxicity phenotypes in model systems associated with Huntington's Disease, Alzheimer's Disease, Parkinson's Disease, and ALS...
  8. Relative Roles of HSP70 and HSP25 in the Modulation of Renal Injury
    Rajasree Sreedharan; Fiscal Year: 2007
    ..The current proposal focuses on two well recognized classes of stress inducible molecular chaperones, heat shock protein 70 and heat shock protein 25 (HSP70 and HSP25) and their function in modulating renal ..
  9. DEVELOPMENT AND MAINTENANCE OF LENS TRANSPARENCY
    John Clark; Fiscal Year: 2009
    ..The lens is used for these studies because of its unique accessibility for the in vivo investigation of protein unfolding and aggregation diseases in aging and the well known protective effects of ?B crystallin. ..
  10. DEVELOPMENT AND MAINTENANCE OF LENS TRANSPARENCY
    JOHN IRWIN CLARK; Fiscal Year: 2010
    ..The lens is used for these studies because of its unique accessibility for the in vivo investigation of protein unfolding and aggregation diseases in aging and the well known protective effects of ?B crystallin. ..
  11. Assembly and Trafficking of Heterotrimeric G Proteins in Vertebrate Photoreceptor
    Maxim Sokolov; Fiscal Year: 2010
    Our long-term research objective is to understand the roles of molecular chaperones in vertebrate photoreceptors...
  12. Assembly and Trafficking of Heterotrimeric G Proteins in Vertebrate Photoreceptor
    Maxim Sokolov; Fiscal Year: 2009
    Our long-term research objective is to understand the roles of molecular chaperones in vertebrate photoreceptors...
  13. Diabetic retinopathy: sigma receptor 1 (??R1) as a novel therapeutic target.
    Sylvia B Smith; Fiscal Year: 2010
    ..The proposed project will extend these findings to understand the mechanism of this protection with the ultimate goal of determining whether (+)- pentazocine may be useful clinically for retinopathy in humans. ..
  14. INTRACELLULAR MECHANISMS OF GLUCOCORTICOID ACTION
    DONALD BENEDICT DEFRANCO; Fiscal Year: 2010
    ..a novel in situ fluorescence recovery after photobleaching (FRAP) assay that led to the identification of molecular chaperones and their associated co-chaperones as nuclear mobility factors for the glucocorticoid receptor (GR)...
  15. INTRACELLULAR MECHANISMS OF GLUCOCORTICOID ACTION
    DONALD BENEDICT DEFRANCO; Fiscal Year: 2010
    ..a novel in situ fluorescence recovery after photobleaching (FRAP) assay that led to the identification of molecular chaperones and their associated co-chaperones as nuclear mobility factors for the glucocorticoid receptor (GR)...
  16. Molecular Basis for Lens Transparency
    Hassane McHaourab; Fiscal Year: 2009
    ..The development of a mechanistic perspective on these molecular transformations is of fundamental biochemical importance and may well have an impact on the development of intervention and therapeutic strategies. ..
  17. CHAPERONES AND PROTEIN BIOSYNTHESIS IN MYELOID CELLS
    WILLIAM NAUSEEF; Fiscal Year: 2000
    ..we speculated that: (1) heme insertion may be a rate-limiting step in the biosynthesis of MPO and (2) molecular chaperones may interact with MPO precursors and facilitate folding events necessary for insertion of heme into heme-..
  18. Heat shock protein 90 antagonist-based therapy of mantle cell lymphoma
    Kapil Bhalla; Fiscal Year: 2009
    ..This strategy involves combinations of treatments with heat shock protein 90 and proteosome inhibitors designed to target protein folding and degradation in MCL cells. ..
  19. Heat shock protein 90 antagonist-based therapy of mantle cell lymphoma
    Kapil Bhalla; Fiscal Year: 2010
    ..This strategy involves combinations of treatments with heat shock protein 90 and proteosome inhibitors designed to target protein folding and degradation in MCL cells. ..
  20. Strucutre of the UNC-45 Chaperone and its Interaction with Skeletal Muscle Myosin
    Sanford I Bernstein; Fiscal Year: 2010
    ..b>Molecular chaperones play key roles in muscle development and function by aiding protein folding and inhibiting protein ..
  21. Hsp90 Regulates EphA2 Signaling and Cell Migration in Glioblastoma
    Jennifer S Isaacs; Fiscal Year: 2010
    ..Our studies will potentially lead to rational molecular-targeted therapeutic approaches to improve GBM survival. ..
  22. Strucutre of the UNC-45 Chaperone and its Interaction with Skeletal Muscle Myosin
    Sanford I Bernstein; Fiscal Year: 2010
    ..b>Molecular chaperones play key roles in muscle development and function by aiding protein folding and inhibiting protein ..
  23. Molecular/Chemistry Chaperones and Hemoglobinopathies
    William Welch; Fiscal Year: 2003
    ..nucleotide sequence of the gene encoding it, it has become clear that a class of protein molecules known as molecular chaperones can modulate the rate of proper protein folding events and determine the degree to which polypeptides enter ..
  24. An Oxygen-Sensing Network Involving Heme and Chaperones
    Li Zhang; Fiscal Year: 2009
    ..Thus, understanding how oxygen is sensed and how heme and molecular chaperones promote oxygen and global gene regulation is important for improving human health...
  25. Structure Function of the Mammalian Stress Proteins
    William Welch; Fiscal Year: 2004
    ..Heat shock proteins, in their role as "molecular chaperones," participate in the synthesis, folding and intracellular transport of many, if not all, polypeptides...