coat protein complex i

Summary

Summary: A protein complex comprised of COATOMER PROTEIN and ADP RIBOSYLATION FACTOR 1. It is involved in transport of vesicles between the ENDOPLASMIC RETICULUM and the GOLGI APPARATUS.

Top Publications

  1. ncbi Dissection of COPI and Arf1 dynamics in vivo and role in Golgi membrane transport
    John F Presley
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nature 417:187-93. 2002
  2. ncbi [Retrograde transport]
    Tomohiko Aoe
    Tanpakushitsu Kakusan Koso 49:914-5. 2004
  3. pmc Structure of coatomer cage proteins and the relationship among COPI, COPII, and clathrin vesicle coats
    Changwook Lee
    Howard Hughes Medical Institute, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA
    Cell 142:123-32. 2010
  4. pmc Rer1p, a retrieval receptor for endoplasmic reticulum membrane proteins, is dynamically localized to the Golgi apparatus by coatomer
    K Sato
    Molecular Membrane Biology Laboratory, RIKEN The Institute of Physical and Chemical Research, Saitama 351 0198, Japan
    J Cell Biol 152:935-44. 2001
  5. pmc Golgicide A reveals essential roles for GBF1 in Golgi assembly and function
    Jose B Saenz
    Department of Pediatrics, Washington University School of Medicine, 660 S Euclid Ave, St Louis, Missouri 63110, USA
    Nat Chem Biol 5:157-65. 2009
  6. ncbi GBF1, a guanine nucleotide exchange factor for ADP-ribosylation factors, is localized to the cis-Golgi and involved in membrane association of the COPI coat
    Kazumasa Kawamoto
    Institute of Biological Sciences and Gene Research Center, University of Tsukuba, Tsukuba Science City, Ibaraki 305 8572, Japan
    Traffic 3:483-95. 2002
  7. doi The COPI system: molecular mechanisms and function
    R Beck
    Yale University School of Medicine, Department of Cell Biology, New Haven, CT 06520, USA
    FEBS Lett 583:2701-9. 2009
  8. pmc COPI recruitment is modulated by a Rab1b-dependent mechanism
    Cecilia Alvarez
    Department of Cell Biology, University of Alabama at Birmingham, 35924, USA
    Mol Biol Cell 14:2116-27. 2003
  9. pmc The COPI vesicle complex binds and moves with survival motor neuron within axons
    Cyril Jayakumar Peter
    Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Hum Mol Genet 20:1701-11. 2011
  10. doi The structures of COPI-coated vesicles reveal alternate coatomer conformations and interactions
    Marco Faini
    Structural and Computational Biology Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Science 336:1451-4. 2012

Research Grants

Detail Information

Publications180 found, 100 shown here

  1. ncbi Dissection of COPI and Arf1 dynamics in vivo and role in Golgi membrane transport
    John F Presley
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nature 417:187-93. 2002
    ..This role for Arf1/coatomer might provide a model for investigating the behaviour of other coat protein systems within cells...
  2. ncbi [Retrograde transport]
    Tomohiko Aoe
    Tanpakushitsu Kakusan Koso 49:914-5. 2004
  3. pmc Structure of coatomer cage proteins and the relationship among COPI, COPII, and clathrin vesicle coats
    Changwook Lee
    Howard Hughes Medical Institute, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA
    Cell 142:123-32. 2010
    ....
  4. pmc Rer1p, a retrieval receptor for endoplasmic reticulum membrane proteins, is dynamically localized to the Golgi apparatus by coatomer
    K Sato
    Molecular Membrane Biology Laboratory, RIKEN The Institute of Physical and Chemical Research, Saitama 351 0198, Japan
    J Cell Biol 152:935-44. 2001
    ..These findings not only give the proof that Rer1p is a novel type of retrieval receptor recognizing the TMD in the Golgi but also indicate that coatomer actively regulates the function and localization of Rer1p...
  5. pmc Golgicide A reveals essential roles for GBF1 in Golgi assembly and function
    Jose B Saenz
    Department of Pediatrics, Washington University School of Medicine, 660 S Euclid Ave, St Louis, Missouri 63110, USA
    Nat Chem Biol 5:157-65. 2009
    ..Collectively, these results highlight the central role for GBF1 in coordinating bidirectional transport and maintaining structural integrity of the Golgi...
  6. ncbi GBF1, a guanine nucleotide exchange factor for ADP-ribosylation factors, is localized to the cis-Golgi and involved in membrane association of the COPI coat
    Kazumasa Kawamoto
    Institute of Biological Sciences and Gene Research Center, University of Tsukuba, Tsukuba Science City, Ibaraki 305 8572, Japan
    Traffic 3:483-95. 2002
    ..These observations indicate that GBF1 is involved in the formation of COPI-coated vesicles from the cis-Golgi or the pre-Golgi intermediate compartment through activating ADP-ribosylation factors...
  7. doi The COPI system: molecular mechanisms and function
    R Beck
    Yale University School of Medicine, Department of Cell Biology, New Haven, CT 06520, USA
    FEBS Lett 583:2701-9. 2009
    ..Specifically, we will weigh the pros and cons of recent data on roles of the small GTP binding protein Arf1, of Arf1GAPs, and lipids during COPI carrier formation...
  8. pmc COPI recruitment is modulated by a Rab1b-dependent mechanism
    Cecilia Alvarez
    Department of Cell Biology, University of Alabama at Birmingham, 35924, USA
    Mol Biol Cell 14:2116-27. 2003
    ..Our data suggest a novel role for Rab1b in ARF1- and GBF1-mediated COPI recruitment pathway...
  9. pmc The COPI vesicle complex binds and moves with survival motor neuron within axons
    Cyril Jayakumar Peter
    Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Hum Mol Genet 20:1701-11. 2011
    ..We propose that neurons utilize the Golgi-associated COPI vesicle to deliver cargoes necessary for motor neuron integrity and function...
  10. doi The structures of COPI-coated vesicles reveal alternate coatomer conformations and interactions
    Marco Faini
    Structural and Computational Biology Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Science 336:1451-4. 2012
    ..This represents a fundamentally different basis for vesicle coat assembly...
  11. ncbi KDEL-cargo regulates interactions between proteins involved in COPI vesicle traffic: measurements in living cells using FRET
    I Majoul
    Department of Neurobiology, Max Planck Institute of Biophysical Chemistry, Gottingen, Germany
    Dev Cell 1:139-53. 2001
    ..Both p24a and p23 interact with ARF1, but only p24 interacts with ARFGAP. These findings suggest a model for how cargo-induced oligomerization of ERD2 regulates its sorting into COPI-coated buds...
  12. pmc Spatiotemporal dynamics of the COPI vesicle machinery
    Markus Elsner
    Cell Biology and Cell Biophysics Programme, EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    EMBO Rep 4:1000-4. 2003
    ..The addition of aluminium fluoride locks coatomer onto Golgi membranes and also decreases the binding kinetics of both ARF1 and ARFGAP1, suggesting that these proteins function in concert to mediate sorting and vesicle formation...
  13. ncbi Overexpression of an ADP-ribosylation factor-guanine nucleotide exchange factor, BIG2, uncouples brefeldin A-induced adaptor protein-1 coat dissociation and membrane tubulation
    Chisa Shinotsuka
    Institute of Biological Sciences and Gene Research Center, University of Tsukuba, 1 1 1 Tennohdai, Tsukuba Science City, Ibaraki 305 8572, Japan
    J Biol Chem 277:9468-73. 2002
    ..These observations indicate that BFA-induced AP-1 dissociation from TGN membranes and tubulation of TGN membranes are not coupled events and suggest that a BFA target other than ARF-GEFs exists in the cell...
  14. pmc GS15 forms a SNARE complex with syntaxin 5, GS28, and Ykt6 and is implicated in traffic in the early cisternae of the Golgi apparatus
    Yue Xu
    Membrane Biology Laboratory, Institute of Molecular and Cell Biology, Singapore 117609, Singapore
    Mol Biol Cell 13:3493-507. 2002
    ....
  15. ncbi Transport between ER and Golgi
    J Klumperman
    Department of Cell Biology, Institute of Biomembranes, Center for Biomedical Genetics, University Medical Center, Utrecht, 3584 CX, The Netherlands
    Curr Opin Cell Biol 12:445-9. 2000
    ..The anterograde membrane flow is compensated for by a retrograde pathway, which, in addition to the recycling of membrane and proteins to the endoplasmic reticulum, may play a role in anterograde cargo concentration...
  16. ncbi Similar subunit interactions contribute to assembly of clathrin adaptor complexes and COPI complex: analysis using yeast three-hybrid system
    H Takatsu
    Institute of Biological Sciences and Gene Experiment Center, University of Tsukuba, Ibaraki, Tsukuba Science City, 305 8572, Japan
    Biochem Biophys Res Commun 284:1083-9. 2001
    ....
  17. pmc Isoform-selective effects of the depletion of ADP-ribosylation factors 1-5 on membrane traffic
    Laura A Volpicelli-Daley
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    Mol Biol Cell 16:4495-508. 2005
    ....
  18. pmc Arf3 is activated uniquely at the trans-Golgi network by brefeldin A-inhibited guanine nucleotide exchange factors
    Florin Manolea
    Department of Cell Biology, University of Alberta, Edmonton, AB, Canada
    Mol Biol Cell 21:1836-49. 2010
    ..These results suggest that Arf3 plays a unique function at the TGN that likely involves recruitment by a specific receptor...
  19. pmc Genetic inactivation of COPI coatomer separately inhibits vesicular stomatitis virus entry and gene expression
    David K Cureton
    Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts, USA
    J Virol 86:655-66. 2012
    ..Our results offer an explanation of why COPI coatomer is frequently identified in screens for cellular factors that support cell invasion by microbial pathogens...
  20. pmc Molecular structure and flexibility of the yeast coatomer as revealed by electron microscopy
    Calvin K Yip
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    J Mol Biol 408:825-31. 2011
    b>Coat protein complex I (COPI)-coated vesicles, one of three major types of vesicular carriers in the cell, mediate the early secretory pathway and retrograde transport from the Golgi to the endoplasmic reticulum...
  21. pmc A COPI coat subunit interacts directly with an early-Golgi localized Arf exchange factor
    Yi Deng
    Cell Biology and Metabolism Program, National Institute of Child Health and Human Development, NIH, Bethesda, Maryland 20892, USA
    EMBO Rep 10:58-64. 2009
    ..A well-studied class of Arf1 effectors comprises the coat complexes, such as the cis-Golgi-localized COPI (coat protein complex I) coat, and trans-Golgi network-endosomal clathrin coats...
  22. ncbi Segregation of COPI-rich and anterograde-cargo-rich domains in endoplasmic-reticulum-to-Golgi transport complexes
    D T Shima
    Cell Biology Laboratory, Imperial Cancer Research Fund, 44, Lincoln s Inn Fields, London, WC2A 3PX, UK
    Curr Biol 9:821-4. 1999
    ..By sequestering retrograde cargo in the anterograde-directed TCs, COPI couples the sorting of ER recycling proteins [10] to the transport of anterograde cargo...
  23. ncbi Identification and characterization of novel isoforms of COP I subunits
    M Futatsumori
    Institute of Biological Sciences and Gene Experiment Center, University of Tsukuba, Tsukuba Science City, Ibaraki 305 8572, Japan
    J Biochem 128:793-801. 2000
    ..These results indicate that gamma2-COP and zeta2-COP can form a COP I-like complex in place of gamma1-COP and zeta1-COP, respectively, and suggest that the COP I complex and the COP I-like complex are functionally redundant...
  24. ncbi Dominant-negative mutant of BIG2, an ARF-guanine nucleotide exchange factor, specifically affects membrane trafficking from the trans-Golgi network through inhibiting membrane association of AP-1 and GGA coat proteins
    Chisa Shinotsuka
    Institute of Biological Sciences and Gene Research Center, University of Tsukuba, Tsukuba Science City, Ibaraki 305 8572, Japan
    Biochem Biophys Res Commun 294:254-60. 2002
    ..These observations indicate that BIG2 is involved in trafficking from the TGN by regulating membrane association of AP-1 and GGA through activating ARF...
  25. ncbi Key components of the fission machinery are interchangeable
    Jia Shu Yang
    Division of Rheumatology, Immunology and Allergy, Brigham and Women s Hospital, and Department of Medicine, Harvard Medical School, Boston, MA 02115, USA
    Nat Cell Biol 8:1376-82. 2006
    ....
  26. pmc Peri-Golgi vesicles contain retrograde but not anterograde proteins consistent with the cisternal progression model of intra-Golgi transport
    J A Martinez-Menarguez
    Department of Cell Biology, School of Medicine, University of Murcia, 30071 Murcia, Spain
    J Cell Biol 155:1213-24. 2001
    ..These data suggest a role of peri-Golgi vesicles in recycling of Golgi residents, rather than an important role in anterograde transport...
  27. pmc A role for BARS at the fission step of COPI vesicle formation from Golgi membrane
    Jia Shu Yang
    Division of Rheumatology, Immunology and Allergy, Brigham and Women s Hospital, Boston, MA 02115, USA
    EMBO J 24:4133-43. 2005
    ..Our findings not only identify a new factor needed for COPI vesicle formation from Golgi membrane but also reveal a surprising mechanism by which the roles of p-coA and GAP are linked in this process...
  28. ncbi Traffic COPs of the early secretory pathway
    C Barlowe
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA
    Traffic 1:371-7. 2000
    ..This review focuses on recent advances in molecular mechanisms underlying coated-vesicle assembly and connections with cellular structures...
  29. pmc The golgi-associated COPI-coated buds and vesicles contain beta/gamma -actin
    F Valderrama
    Departament de Biologia Cellular, Facultat de Medicina, Institut d Investigacions Biomediques August Pi i Sunyer IDIBAPS, Universitat de Barcelona, 08036 Barcelona, Spain
    Proc Natl Acad Sci U S A 97:1560-5. 2000
    ....
  30. pmc Intracellular phospholipase A1gamma (iPLA1gamma) is a novel factor involved in coat protein complex I- and Rab6-independent retrograde transport between the endoplasmic reticulum and the Golgi complex
    Rei K Morikawa
    Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan
    J Biol Chem 284:26620-30. 2009
    ..Thus, iPLA(1)gamma is a novel membrane transport factor that contributes to a specific Golgi-to-ER retrograde pathway distinct from presently characterized COPI- and Rab6-dependent pathways...
  31. pmc Functional genomic screen reveals genes involved in lipid-droplet formation and utilization
    Yi Guo
    Department of Biochemistry and Biophysics, University of California, San Francisco, California 94158, USA
    Nature 453:657-61. 2008
    ..These phenotypes are conserved in mammalian cells, suggesting that insights from these studies are likely to be central to our understanding of human diseases involving excessive lipid storage...
  32. pmc Models for the functions of Arf GAPs
    Michael P East
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322 3050, USA
    Semin Cell Dev Biol 22:3-9. 2011
    ....
  33. doi Physical aspects of COPI vesicle formation
    Mathieu Pinot
    UMR 144 CNRS Institut Curie, 75248 Paris Cedex 05, France
    Mol Membr Biol 27:428-42. 2010
    ..b>Coat protein complex I (COPI) vesicles shuttle between the Golgi apparatus and the endoplasmic reticulum and between Golgi stacks...
  34. pmc COPI complex is a regulator of lipid homeostasis
    Mathias Beller
    Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA
    PLoS Biol 6:e292. 2008
    ..Those include the vesicle-mediated Coat Protein Complex I (COPI) transport complex, which is required for limiting lipid storage...
  35. pmc Distinct functions for Arf guanine nucleotide exchange factors at the Golgi complex: GBF1 and BIGs are required for assembly and maintenance of the Golgi stack and trans-Golgi network, respectively
    Florin Manolea
    Department of Cell Biology, University of Alberta, Edmonton, AB, T6G 2H7 Canada
    Mol Biol Cell 19:523-35. 2008
    ....
  36. pmc Cog3p depletion blocks vesicle-mediated Golgi retrograde trafficking in HeLa cells
    Sergey N Zolov
    Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA
    J Cell Biol 168:747-59. 2005
    ..In a contrast, Cog3p KD resulted in inhibition of retrograde trafficking of the Shiga toxin. Furthermore, the mammalian COG complex physically interacts with GS28 and COPI and specifically binds to isolated CCD vesicles...
  37. pmc ARFGAP1 plays a central role in coupling COPI cargo sorting with vesicle formation
    Stella Y Lee
    Division of Rheumatology, Immunology, and Allergy, Brigham and Women s Hospital, and Department of Medicine, Harvard Medical School, Boston, MA 02115
    J Cell Biol 168:281-90. 2005
    ..Together, these findings indicate that GAP plays a central role in coupling cargo sorting and vesicle formation, with implications for simplifying models to describe how these two processes are coupled during COPI transport...
  38. doi The evolving understanding of COPI vesicle formation
    Victor W Hsu
    Division of Rheumatology, Immunology and Allergy, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Rev Mol Cell Biol 10:360-4. 2009
    ..Here, we discuss emerging evidence that suggests the need to revise some long-held views on how COPI vesicle formation is achieved...
  39. pmc ADP-ribosylation factor/COPI-dependent events at the endoplasmic reticulum-Golgi interface are regulated by the guanine nucleotide exchange factor GBF1
    Rafael Garcia-Mata
    Department of Cell Biology, University of Alabama at Birmingham, 35924, USA
    Mol Biol Cell 14:2250-61. 2003
    ..Our findings imply that the continuous recruitment of GBF1 to spatially differentiated membrane domains is required for sustained membrane remodeling that underlies membrane traffic and Golgi biogenesis...
  40. ncbi Differential requirements for COPI transport during vertebrate early development
    Pedro Coutinho
    Division of Developmental Biology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom
    Dev Cell 7:547-58. 2004
    ..Moreover, we note that the mRNA encoding alpha coatomer is strikingly upregulated in notochord progenitors, and we present data suggesting that alpha coatomer transcription is tuned to activity- and cell type-specific secretory loads...
  41. pmc The COG and COPI complexes interact to control the abundance of GEARs, a subset of Golgi integral membrane proteins
    Toshihiko Oka
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Mol Biol Cell 15:2423-35. 2004
    ..COG and COPI may work in concert to ensure the proper retention or retrieval of a subset of proteins in the Golgi, and COG helps prevent the endoplasmic reticulum accumulation and degradation of some GEARs...
  42. ncbi Gamma-COP appendage domain - structure and function
    Peter J Watson
    Cambridge Institute for Medical Research and Department of Clinical Biochemistry, University of Cambridge, Hills Road, Cambridge CB2 2XY, UK
    Traffic 5:79-88. 2004
    ..On the basis of mutations in the yeast homologue of gamma-COP, Sec21p, a second binding site is proposed to exist on the gamma-COP appendage that interacts with the alpha,beta',epsilon COPI subcomplex...
  43. pmc Functional reconstitution of COPI coat assembly and disassembly using chemically defined components
    Constanze Reinhard
    Biochemie Zentrum Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    Proc Natl Acad Sci U S A 100:8253-7. 2003
    ..Bourgoin, S., Randazzo, P. A., et al. (2002) J. Cell Biol. 159, 69-78]. Thus, a complete round of COPI coat assembly and disassembly has been reconstituted with purified components defining the core machinery of COPI vesicle biogenesis...
  44. ncbi Lipid packing sensed by ArfGAP1 couples COPI coat disassembly to membrane bilayer curvature
    Joëlle Bigay
    Institut de Pharmacologie Moleculaire et Cellulaire, CNRS, 660 route des Lucioles, 06560 Valbonne Sophia Antipolis, France
    Nature 426:563-6. 2003
    ..This leads to a model for COPI dynamics in which GTP hydrolysis in Arf1 is organized temporally and spatially according to the changes in lipid packing induced by the coat...
  45. ncbi ER-to-Golgi transport: COP I and COP II function (Review)
    Rainer Duden
    Cambridge Institute for Medical Research, Department of Clinical Biochemistry, University of Cambridge, Hills Road, Cambridge CB2 2XY, UK
    Mol Membr Biol 20:197-207. 2003
    ..Additionally, COP I coat proteins have complex functions in intra-Golgi trafficking and in maintaining the normal structure of the mammalian interphase Golgi complex...
  46. ncbi Dissection of membrane dynamics of the ARF-guanine nucleotide exchange factor GBF1
    Tomasz Szul
    Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL 35924, USA
    Traffic 6:374-85. 2005
    ..Our findings imply that continuous cycles of recruitment and dissociation of GBF1 to membranes are required for sustained ARF activation and COP I recruitment that underlies ER-Golgi traffic...
  47. pmc Two human ARFGAPs associated with COP-I-coated vesicles
    Gabriella Frigerio
    Department of Clinical Biochemistry, Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge CB2 2XY, United Kingdom
    Traffic 8:1644-55. 2007
    ..However, silencing all three ARFGAPs causes cell death. Our data provide strong evidence that ARFGAP2 and ARFGAP3 function in COP I traffic...
  48. ncbi Dissecting the role of the ARF guanine nucleotide exchange factor GBF1 in Golgi biogenesis and protein trafficking
    Tomasz Szul
    Department of Cell Biology, University of Alabama at Birmingham, 1918 University Blvd, Birmingham, AL 35924, USA
    J Cell Sci 120:3929-40. 2007
    ..However, the trafficking of transmembrane proteins through the existing pathway requires GBF1-mediated ARF activation and COPI recruitment...
  49. pmc Rab1b interacts with GBF1 and modulates both ARF1 dynamics and COPI association
    Pablo Monetta
    Centro de Investigaciones en Bioquimica Clinica e Inmunologia, Departamento Bioquimica Clinica, Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, Cordoba 5000, Argentina
    Mol Biol Cell 18:2400-10. 2007
    ..Our data support a model where Rab1b-GTP induces GBF1 recruitment at the ERES interface and at the Golgi complex where it is required for COPII/COPI exchange or COPI vesicle formation, respectively...
  50. pmc COPI coatomer complex proteins facilitate the translocation of anthrax lethal factor across vesicular membranes in vitro
    Alfred G Tamayo
    Section of Molecular Medicine, Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA
    Proc Natl Acad Sci U S A 105:5254-9. 2008
    ..This facilitated delivery appears to use a mechanism that is analogous to that of DT entry...
  51. ncbi Quantitative proteomics analysis of the secretory pathway
    Annalyn Gilchrist
    Department of Anatomy and Cell Biology, McGill University, 3640 University Street, Montreal, Quebec H3A 2B2, Canada
    Cell 127:1265-81. 2006
    ..Of these, 230 had their subcellular location deduced by proteomics. This study provides a comprehensive catalog of the ER and Golgi proteomes with insight into their identity and function...
  52. pmc Involvement of specific COPI subunits in protein sorting from the late endosome to the vacuole in yeast
    Galina Gabriely
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Mol Cell Biol 27:526-40. 2007
    ..Together, our findings suggest that certain COPI subunits could have a direct role in vacuolar protein sorting to the MVB compartment...
  53. doi Depletion of beta-COP reveals a role for COP-I in compartmentalization of secretory compartments and in biosynthetic transport of caveolin-1
    Melanie L Styers
    Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Am J Physiol Cell Physiol 294:C1485-98. 2008
    ..Our findings suggest that COP-I is required for the compartmentalization of the ERGIC, Golgi, TGN, and recycling endosomes and that COP-I plays a novel role in the biosynthetic transport of Cav1...
  54. doi Scyl1, mutated in a recessive form of spinocerebellar neurodegeneration, regulates COPI-mediated retrograde traffic
    Jonathon L Burman
    Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, QC H3A 2B4, Canada
    J Biol Chem 283:22774-86. 2008
    ..Our data demonstrate a function for Scyl1 as an accessory factor in COPI trafficking and suggest for the first time that alterations in the COPI pathway result in neurodegenerative disease...
  55. ncbi Structural design of cage and coat scaffolds that direct membrane traffic
    Scott M Stagg
    Department of Cell, The Scripps Research Institute, La Jolla, CA 92037, USA
    Curr Opin Struct Biol 17:221-8. 2007
    ..These studies emphasize molecular and structural principles that reflect the properties of self-assembling nanomachines to regulate cargo capacity in trafficking pathways...
  56. ncbi COPI-mediated transport
    J Bethune
    Biochemie Zentrum, University of Heidelberg, Im Neuenheimer Feld 328, D 69120, Heidelberg, Germany
    J Membr Biol 211:65-79. 2006
    ..Finally, we describe the striking similarities that exist between the COPI system and the two other characterized types of vesicular carriers: COPII- and clathrin-coated vesicles...
  57. pmc COPI activity coupled with fatty acid biosynthesis is required for viral replication
    Sara Cherry
    University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, United States of America
    PLoS Pathog 2:e102. 2006
    ..screening, we found that this step in the viral lifecycle requires at least two host encoded pathways: the coat protein complex I (COPI) coatamer and fatty acid biosynthesis...
  58. ncbi A viral protein that blocks Arf1-mediated COP-I assembly by inhibiting the guanine nucleotide exchange factor GBF1
    Els Wessels
    Department of Medical Microbiology, Radboud University Nijmegen Medical Centre, Nijmegen Centre for Molecular Life Sciences, PO Box 9101, 6500 HB Nijmegen, The Netherlands
    Dev Cell 11:191-201. 2006
    ..By specifically interfering with GBF1-mediated Arf1 activation, 3A may prove a valuable tool in dissecting the early steps of the secretory pathway...
  59. pmc Mutants in trs120 disrupt traffic from the early endosome to the late Golgi
    Huaqing Cai
    Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06519, USA
    J Cell Biol 171:823-33. 2005
    ..Furthermore, we demonstrate that Trs120p largely colocalizes with the late Golgi marker Sec7p. Our findings imply that Trs120p is required for vesicle traffic from the early endosome to the late Golgi...
  60. pmc COPI coat assembly occurs on liquid-disordered domains and the associated membrane deformations are limited by membrane tension
    Jean Baptiste Manneville
    Unite Mixte de Recherche 144, Centre National de la Recherche Scientifique Institut Curie, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    Proc Natl Acad Sci U S A 105:16946-51. 2008
    ..These findings suggest that the COPI coat is adapted to liquid disordered membrane domains where it could promote lipid sorting and that its mechanical effects can be tuned by membrane tension...
  61. pmc ArfGAP1 dynamics and its role in COPI coat assembly on Golgi membranes of living cells
    Wei Liu
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 168:1053-63. 2005
    ..These data suggest that ArfGAP1, coatomer and Arf1 play interdependent roles in the assembly-disassembly cycle of the COPI coat in vivo...
  62. ncbi Three ways to make a vesicle
    T Kirchhausen
    Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nat Rev Mol Cell Biol 1:187-98. 2000
    ..A general model to explain the formation of coated vesicles is starting to emerge but the picture is more complex than we had imagined...
  63. pmc ARF-GAP-mediated interaction between the ER-Golgi v-SNAREs and the COPI coat
    Ulrike Rein
    Friedrich Miescher Laboratory, Max Planck Society, D 72076 Tubingen, Germany
    J Cell Biol 157:395-404. 2002
    ..The mechanisms by which v-SNAREs interact with COPI and COPII coat proteins seem to be different and may play a key role in determining specificity in vesicle budding...
  64. pmc Differential requirements for COPI coats in formation of replication complexes among three genera of Picornaviridae
    Elena V Gazina
    Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Victoria 3004, Australia
    J Virol 76:11113-22. 2002
    ..ParV1 RCs are formed from COPI-containing membranes but COPI is unlikely to be directly involved in their formation, whereas formation of EV11 RCs appears to be dependent on COPI association with membranes...
  65. ncbi ARF1 regulatory factors and COPI vesicle formation
    Anne Spang
    Friedrich Miescher Laboratorium, Max Planck Gesellschaft, Spemannstrasse 39 D 72076 Tübingen, Germany
    Curr Opin Cell Biol 14:423-7. 2002
    ..clear that cargo might not simply be a passive passenger, and that ADP-ribosylation factor (ARF) GAPs are not only GTPase-activating proteins for ARF, but might play crucial roles in regulating coat protein complex I vesicle formation.
  66. ncbi Breaking the COPI monopoly on Golgi recycling
    B Storrie
    Dept of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
    Trends Cell Biol 10:385-91. 2000
    ..As signal-mediated COPI-dependent recycling also involves the concentration of resident proteins into retrograde COPI vesicles, the main bulk of lipids must be recycled, possibly through a COPI-independent pathway...
  67. ncbi Imaging of procollagen transport reveals COPI-dependent cargo sorting during ER-to-Golgi transport in mammalian cells
    David J Stephens
    Cell Biology and Cell Biophysics Programme, EMBL Heidelberg, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    J Cell Sci 115:1149-60. 2002
    ..These data reveal the existence of an early COPI-dependent, pre-Golgi cargo sorting step in mammalian cells...
  68. pmc Localization of large ADP-ribosylation factor-guanine nucleotide exchange factors to different Golgi compartments: evidence for distinct functions in protein traffic
    Xinhua Zhao
    Department of Cell Biology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    Mol Biol Cell 13:119-33. 2002
    ..In agreement with this possibility, COPI overlapped to a greater extent with GBF1 (64%) than BIG1 (31%), whereas clathrin showed limited overlap with BIG1, and virtually none with GBF1...
  69. pmc The Sec34/Sec35p complex, a Ypt1p effector required for retrograde intra-Golgi trafficking, interacts with Golgi SNAREs and COPI vesicle coat proteins
    Elena S Suvorova
    Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA
    J Cell Biol 157:631-43. 2002
    ..We propose that the Sec34/35 protein complex acts as a tether that connects cis-Golgi membranes and COPI-coated, retrogradely targeted intra-Golgi vesicles...
  70. pmc Evidence that Golgi structure depends on a p115 activity that is independent of the vesicle tether components giantin and GM130
    M A Puthenveedu
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213, USA
    J Cell Biol 155:227-38. 2001
    ..These observations indicate that inhibition of p115 can induce a mitotic-like Golgi disassembly, but its essential role in Golgi structure is independent of its Golgi-localized binding partners giantin and GM130...
  71. ncbi COPI vesicles accumulating in the presence of a GTP restricted arf1 mutant are depleted of anterograde and retrograde cargo
    R Pepperkok
    Cell Biophysics and Cell Biology Program, EMBL Heidelberg, Meyerhofstr 1, Germany
    J Cell Sci 113:135-44. 2000
    ....
  72. pmc Identification of Sec36p, Sec37p, and Sec38p: components of yeast complex that contains Sec34p and Sec35p
    Rachna J Ram
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
    Mol Biol Cell 13:1484-500. 2002
    ..These tests indicate two general types of subunits: Sec34p, Sec35p, Sec36p, and Sec38p seem to form the essential core of a complex to which Sec37p, Cod4p, Cod5p, and Dor1p seem to be peripherally attached...
  73. ncbi Golgi architecture and inheritance
    James Shorter
    Annu Rev Cell Dev Biol 18:379-420. 2002
    ..This structural framework may be instructive for Golgi biogenesis and may encode sufficient information to ensure accurate Golgi inheritance, thereby helping to resolve some of the current discrepancies between different workers...
  74. ncbi Yeast ER-Golgi v-SNAREs Bos1p and Bet1p differ in steady-state localization and targeting
    D Ossipov
    Department of Molecular Genetics, Max Planck Institute for Biophysical Chemistry, D 37070 Gottingen, Germany
    J Cell Sci 112:4135-42. 1999
    ..Bet1-alpha is an early Golgi protein and it does not change its localization under conditions when other recycling Golgi proteins can be trapped in the ER...
  75. pmc Reevaluation of the effects of brefeldin A on plant cells using tobacco Bright Yellow 2 cells expressing Golgi-targeted green fluorescent protein and COPI antisera
    Christophe Ritzenthaler
    Institut de Biologie Moleculaire des Plantes, 67084 Strasbourg Cedex, France
    Plant Cell 14:237-61. 2002
    ....
  76. ncbi Presenilin-1-mediated retention of APP derivatives in early biosynthetic compartments
    Marloes Réchards
    Cell Microscopy Center, Department of Cell Biology, University Medical Center and Institute for Biomembranes, 3584 CX Utrecht, The Netherlands
    Traffic 7:354-64. 2006
    ..Malfunctioning of PS-1 in this role may have important consequences for the progress of AD...
  77. ncbi H-Ras does not need COP I- or COP II-dependent vesicular transport to reach the plasma membrane
    Hui Zheng
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa 50011, USA
    J Biol Chem 282:25760-8. 2007
    ..Thus, H-Ras has two simultaneous but distinct means of transport and need not depend on vesicular trafficking for its delivery to the plasma membrane...
  78. pmc Traffic of Kv4 K+ channels mediated by KChIP1 is via a novel post-ER vesicular pathway
    Burcu Hasdemir
    The Physiological Laboratory, School of Biomedical Sciences, University of Liverpool, Liverpool L69 3BX, England, UK
    J Cell Biol 171:459-69. 2005
    ..The EF-hand mutant had no effect on general exocytic traffic. Traffic of Kv4.2 was coat protein complex I (COPI)-dependent, but KChIP1-containing vesicles were not COPII-coated, and expression of a GTP-loaded ..
  79. ncbi The role of ARF1 and rab GTPases in polarization of the Golgi stack
    Serguei I Bannykh
    Department of Pathology, Yale University Medical School, 310 Cedar Street, New Haven, CT 06520, USA
    Traffic 6:803-19. 2005
    ....
  80. ncbi Uncoupling of brefeldin a-mediated coatomer protein complex-I dissociation from Golgi redistribution
    Eran Barzilay
    Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv 69978, Israel
    Traffic 6:794-802. 2005
    ..Taken together, we propose that monensin inhibits membrane fusion processes in the presence or absence of BFA...
  81. pmc Golgi-to-endoplasmic reticulum (ER) retrograde traffic in yeast requires Dsl1p, a component of the ER target site that interacts with a COPI coat subunit
    B A Reilly
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Mol Biol Cell 12:3783-96. 2001
    ..Last, we show that Dsl1p interacts with the delta-subunit of the retrograde COPI coat, Ret2p, and discuss possible roles for this interaction...
  82. pmc PGY repeats and N-glycans govern the trafficking of paranodin and its selective association with contactin and neurofascin-155
    Carine Bonnon
    Neurobiologie des Interactions Cellulaires et Neurophysiopathologie, CNRS UMR 6184, Universite de la Mediterranee, Institut Jean Roche, 13916 Marseille Cedex 20, France
    Mol Biol Cell 18:229-41. 2007
    ..Thus, the unconventional processing of paranodin and contactin may determine the selective association of axo-glial complexes at paranodes...
  83. ncbi Evidence for a COP-I-independent transport route from the Golgi complex to the endoplasmic reticulum
    A Girod
    Cell Biology and Biophysics Programme, EMBL Heidelberg, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Nat Cell Biol 1:423-30. 1999
    ..The COP-I-independent pathway is specifically regulated by Rab6 and is used by Golgi glycosylation enzymes and Shiga toxin/Shiga-like toxin-1...
  84. ncbi Retrograde transport of protein toxins under conditions of COPI dysfunction
    Alice Chen
    Molecular and Cell Biology Department, FO31, University of Texas at Dallas, Box 830688, Richardson, TX 75083 0688, USA
    Biochim Biophys Acta 1589:124-39. 2002
    ..In addition, the results suggest that certain toxins containing a KDEL motif may use either the COPI-dependent or COPI-independent pathway of retrograde transport...
  85. ncbi Site-specific photocrosslinking to probe interactions of Arf1 with proteins involved in budding of COPI vesicles
    K D Fischer
    Biochemie Zentrum Heidelberg BZH, University of Heidelberg, Im Neuenheimer Feld 328, Heidelberg, 69120, Germany
    Methods 20:455-64. 2000
    ....
  86. pmc Arf1-GTP-induced tubule formation suggests a function of Arf family proteins in curvature acquisition at sites of vesicle budding
    Michael Krauss
    Institute of Chemistry and Biochemistry, Department of Membrane Biochemistry, Freie Universitat Berlin, 14195 Berlin, Germany
    J Biol Chem 283:27717-23. 2008
    ..Our data indicate that GTP-controlled local induction of high curvature membranes is an important property of Arf1 that might be shared by a subgroup of Arf/Arl family GTPases...
  87. doi Signal-mediated dynamic retention of glycosyltransferases in the Golgi
    Linna Tu
    Department of Biology, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, Special Administrative Region SAR of the People s Republic of China
    Science 321:404-7. 2008
    ..The cytoplasmic tails play a role in the localization of glycosyltransferases, and coat protein complex I (COPI) vesicle-mediated retrograde transport is also involved in their Golgi localization...
  88. pmc COPI vesicle transport is a common requirement for tube expansion in Drosophila
    Satish Arcot Jayaram
    Department of Developmental Biology, Wenner Gren Institute, Stockholm University, Stockholm, Sweden
    PLoS ONE 3:e1964. 2008
    ..In the trachea, COPII anterograde transport of luminal proteins is required for extracellular matrix assembly and the concurrent tube expansion...
  89. pmc Golgi cisternal unstacking stimulates COPI vesicle budding and protein transport
    Yanzhuang Wang
    Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, Michigan, USA
    PLoS ONE 3:e1647. 2008
    ..The results further suggest that at the onset of mitosis, unstacking of cisternae allows extensive and rapid vesiculation of the Golgi in preparation for its subsequent partitioning...
  90. doi Adaptor protein Ruk/CIN85 is associated with a subset of COPI-coated membranes of the Golgi complex
    Serhiy Havrylov
    School of Biosciences, Cardiff University, Museum Avenue, Cardiff CF10 3US, Wales, UK
    Traffic 9:798-812. 2008
    ..Only a small pool of Ruk/CIN85 is present in compartments involved in clathrin-mediated endocytosis and sorting. These results suggest that endogenous Ruk/CIN85 may be involved in regulation of specific membrane trafficking processes...
  91. ncbi Sec7p directs the transitions required for yeast Golgi biogenesis
    S B Deitz
    Program in Molecular Biology and CU Cancer Center, Department of Cellular and Structural Biology, Box B 111, University of Colorado Health Sciences Center, Denver, CO 80262, USA
    Traffic 1:172-83. 2000
    ....
  92. pmc Alternate routes for drug delivery to the cell interior: pathways to the Golgi apparatus and endoplasmic reticulum
    Maria Teresa Tarrago-Trani
    Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
    Adv Drug Deliv Rev 59:782-97. 2007
    ..We highlight the retrograde trafficking pathway illustrated by Shiga toxin and Shiga-like toxin, and the potential role of the B subunit of these toxins as carriers of drugs, antigens and imaging agents...
  93. pmc Diacylglycerol is required for the formation of COPI vesicles in the Golgi-to-ER transport pathway
    Inés Fernández-Ulibarri
    Departament de Biologia Cellular i Anatomia Patologica, Facultat de Medicina and Institut d Investigacions Biomèdiques August Pi i Sunyer, Universitat de Barcelona, 08036 Barcelona, Spain
    Mol Biol Cell 18:3250-63. 2007
    ..Our findings implicate diacylglycerol in the retrograde transport of proteins from Golgi to the ER and suggest that it plays a critical role at a late stage of COPI vesicle formation...
  94. ncbi Multiple and stepwise interactions between coatomer and ADP-ribosylation factor-1 (Arf1)-GTP
    Zhe Sun
    Biochemie Zentrum der Universität Heidelberg BZH, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    Traffic 8:582-93. 2007
    ..These interactions are located within the core, adaptor-like domain of coatomer, indicating an organizational similarity between the COP I coat and clathrin adaptor complexes...
  95. ncbi Peroxisomal membrane proteins are properly targeted to peroxisomes in the absence of COPI- and COPII-mediated vesicular transport
    T Voorn-Brouwer
    Department of Biochemistry, University of Amsterdam, Academic Medical Center, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands
    J Cell Sci 114:2199-204. 2001
    ..Together, these data fail to provide any evidence for a role of the endoplasmic reticulum in peroxisome biogenesis...
  96. pmc Mutations in a highly conserved region of the Arf1p activator GEA2 block anterograde Golgi transport but not COPI recruitment to membranes
    Sei Kyoung Park
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Biol Cell 16:3786-99. 2005
    ..Hence this region of Gea2p upstream of the Sec7 domain plays a role in anterograde transport that is independent of its role in recruiting COPI for retrograde transport, at least of a subset of Golgi-ER cargo...
  97. ncbi Rab2 interacts directly with atypical protein kinase C (aPKC) iota/lambda and inhibits aPKCiota/lambda-dependent glyceraldehyde-3-phosphate dehydrogenase phosphorylation
    Ellen J Tisdale
    Department of Pharmacology, School of Medicine, Wayne State University, Detroit, Michigan 48201, USA
    J Biol Chem 278:52524-30. 2003
    ..These results suggest that a downstream effector recruited to the VTC stimulates PKCiota/lambda-mediated GAPDH phosphorylation by alleviating the inhibition imposed by Rab2-PKCiota/lambda interaction...
  98. ncbi Localization of GDP-mannose transporter in the Golgi requires retrieval to the endoplasmic reticulum depending on its cytoplasmic tail and coatomer
    Masato Abe
    Department of Biotechnology, University of Tokyo, Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    J Cell Sci 117:5687-96. 2004
    ..Our results indicate that GMT escapes from delivery to the vacuole by recycling to the endoplasmic reticulum and retrieval requires the lysine-rich C-terminal tail that can bind to the COPI coat...
  99. pmc The GTPase ARF1p controls the sequence-specific vacuolar sorting route to the lytic vacuole
    Peter Pimpl
    Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, United Kingdom
    Plant Cell 15:1242-56. 2003
    ..This finding suggests that ARF1p influences the BP80-mediated transport route to the vacuole in addition to transport steps of the default secretory pathway to the cell surface...
  100. pmc Arf1p provides an unexpected link between COPI vesicles and mRNA in Saccharomyces cerevisiae
    Mark Trautwein
    Friedrich Miescher Laboratorium, Max Planck Gesellschaft, D 72076 Tubingen, Germany
    Mol Biol Cell 15:5021-37. 2004
    ..Hereby acts the SHE machinery in long-range mRNA transport, whereas COPI vesicles could act as short-range and localization vehicles. The endoplasmic reticulum (ER)-Golgi shuttle might be involved in concentrating mRNA at the ER...
  101. ncbi COP and clathrin-coated vesicle budding: different pathways, common approaches
    Harvey T McMahon
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK
    Curr Opin Cell Biol 16:379-91. 2004
    ..These themes point to the way in which evolutionarily conserved features underpin these diverse pathways...

Research Grants94

  1. MOLECULAR GENETIC ANALYSIS OF FUNGAL CIRCADIAN RHYTHMS
    Deborah Bell Pedersen; Fiscal Year: 2007
    ..Our studies will have a significant impact on understanding how circadian clocks control overt rhythmicity and will potentially provide novel approaches for therapies for human diseases that result from circadian dysfunction. ..
  2. Neuroprotective Vaccination for Parkinson's Disease
    R Mosley; Fiscal Year: 2005
    ..These approaches could prove useful for treatment of human PD. ..
  3. BIOGENESIS OF TRANSPORT VESICLES COATED BY COPI
    Victor Hsu; Fiscal Year: 2007
    ..Thus, as defects in intracellular transport pathways are being appreciated as the cause for a growing number of human diseases, our findings will likely be broadly applicable to understanding and treating these diseases. ..
  4. BIOGENESIS OF TRANSPORT VESICLES COATED BY COPI
    Victor Hsu; Fiscal Year: 2006
    ....
  5. Cargo sorting during endocytic recycling
    Victor Hsu; Fiscal Year: 2007
    ..abstract_text> ..
  6. Host proteins in Vaccinia viral membrane biogenesis
    Victor Hsu; Fiscal Year: 2004
    ..abstract_text> ..
  7. Cargo sorting during endocytic recycling
    Victor Hsu; Fiscal Year: 2007
    ..abstract_text> ..
  8. ARF regulators in endocytic transport
    Victor W Hsu; Fiscal Year: 2010
    ..This understanding will be broadly applicable to understanding human diseases, as many are now appreciated to arise because of defects in protein transport within the cell. ..
  9. BIOGENESIS OF TRANSPORT VESICLES COATED BY COPI
    Victor W Hsu; Fiscal Year: 2010
    ..Thus, as defects in intracellular transport pathways are being appreciated as the cause for a growing number of human diseases, our findings will likely be broadly applicable to understanding and treating these diseases. ..
  10. BIOGENESIS OF TRANSPORT VESICLES COATED BY COPI
    Victor Hsu; Fiscal Year: 2009
    ..Our results will likely contribute to a basic understanding of transport mechanisms within the cell, and also shed insight into the regulation of iron metabolism. ..
  11. ARF regulators in endocytic transport
    Victor W Hsu; Fiscal Year: 2011
    ..This understanding will be broadly applicable to understanding human diseases, as many are now appreciated to arise because of defects in protein transport within the cell. ..
  12. MOLECULAR INTERACTIONS IN CLATHRIN COATED VESICLES
    Tomas Kirchhausen; Fiscal Year: 2010
    ..By the end of this grant period, we aim to be able to study the dynamics of clathrin coat assembly and disassembly in a reconstituted system, in parallel with our work on coat dynamics in living cells. ..
  13. Influence of iron status on the neurotoxicity of inhaled manganese
    Marianne Wessling Resnick; Fiscal Year: 2010
    ....
  14. The role of autophagy in innate anti-viral immunity in Drosophila
    Sara Cherry; Fiscal Year: 2010
    ..The identification of new host factors amenable to inhibition may lead to targets for much-needed antiviral therapeutics. ..
  15. MOLECULAR INTERACTIONS IN CLATHRIN COATED VESICLES
    Tomas Kirchhausen; Fiscal Year: 2006
    ..abstract_text> ..
  16. EXOCRINE PANCREATIC ZYMOGEN ACTIVATION
    Fred S Gorelick; Fiscal Year: 2010
    ....
  17. Chemical Genetics of Iron Transport
    Marianne Wessling Resnick; Fiscal Year: 2010
    ....
  18. Mechanisms of COPII-dependent Transport
    Charles Barlowe; Fiscal Year: 2007
    ..Therefore, the proposed studies are basic for understanding numerous health related issues and are specifically relevant to understanding cholesterol regulation, Alzheimer's disease and cystic fibrosis. ..
  19. Fc Receptor Signaling in Vaccine Design for the Elderly
    Biao Zheng; Fiscal Year: 2009
    ..Study the modulation of immune responses by selective signaling Fc receptors Aim 3. Determine the effectiveness of 1C vaccines in overcoming age-related immune deficiency. ..
  20. Mechanisms of COPII-dependent Transport
    Charles Barlowe; Fiscal Year: 2006
    ..Therefore, these studies are basic for illuminating endocrine and exocrine secretion and will be of broad importance to medicine. ..
  21. MOLECULAR INTERACTIONS IN CLATHRIN COATED VESICLES
    Tomas Kirchhausen; Fiscal Year: 2002
    ..We thus hope to provide a molecular picture of the key steps in sorting and coat assembly. ..
  22. CORE--METAL ANALYSIS AND MOLECULAR GENETICS
    MELVIN BILLINGSLEY; Fiscal Year: 2002
    ..abstract_text> ..
  23. EXOCRINE PANCREATIC ZYMOGEN ACTIVATION
    Fred Gorelick; Fiscal Year: 2001
    ..Finally, the importance of compartmental acidification and vacuolar ATPases in this pathologic processing will be tested. ..
  24. IMMUNOSENESCENCE AND ALTERED GERMINAL CENTER RESPONSE
    Biao Zheng; Fiscal Year: 2003
    ..These approaches will make possible to systemically study the effect of aging on germinal center response and its consequence. ..
  25. Age-Related Differences in Cartilage OP-1
    Susan Chubinskaya; Fiscal Year: 2005
    ..This study will contribute to the understanding of the mechanisms of cartilage repair/anabolism. ..
  26. VTC Events Mediated by the Rab2 Complex
    Ellen J Tisdale; Fiscal Year: 2010
    ..The studies proposed in this application will develop a new and novel paradigm that couples Rab2-Apkc lA-GAPDH dysfunction with pathological consequences. ..
  27. Influence of iron status on the neurotoxicity of inhaled manganese
    Marianne Wessling Resnick; Fiscal Year: 2009
    ....
  28. EXOCRINE PANCREATIC ZYMOGEN ACTIVATION
    Fred Gorelick; Fiscal Year: 2009
    ....
  29. Influence of iron status on the neurotoxicity of inhaled manganese
    Marianne Wessling Resnick; Fiscal Year: 2009
    ....
  30. EXOCRINE PANCREATIC ZYMOGEN ACTIVATION
    Fred Gorelick; Fiscal Year: 2006
    ..Further, we will examine the effects of the sensitizing agents on cell injury and the trafficking of active enzymes. ..
  31. Fluorescence-based screen to probe hepcidin-ferroportin interactions
    Marianne Wessling Resnick; Fiscal Year: 2006
    ..The project has broader applicability in the development of HTS to assay ligand-receptor interactions as well as representing a major advance towards the discovery of small molecule probes of iron transport. ..
  32. Chemical Genetics of Iron Transport
    Marianne Wessling Resnick; Fiscal Year: 2007
    ....
  33. The role of autophagy in innate anti-viral immunity in Drosophila
    Sara Cherry; Fiscal Year: 2007
    ..The identification of new host factors amenable to inhibition may lead to targets for much-needed antiviral therapeutics. ..
  34. Iron transport and its regulation in C. elegans
    Marianne Wessling Resnick; Fiscal Year: 2002
    ..elegans; 3) Use genetic selection to establish transport mutants useful for the elucidation of additional proteins involved in iron import and/or export and the factors that regulate these processes. ..
  35. Mn transport in the Belgrade rat
    Marianne Wessling Resnick; Fiscal Year: 2003
    ..The Belgrade rat provides the model system to address test our hypothesis. ..
  36. TRAINING PROGRAM IN INVESTIGATIVE GASTROENTEROLOGY
    Fred Gorelick; Fiscal Year: 2007
    ..Fred Gorelick will become the Program Director, and Dr. Henry Binder, will become the Associate Director. The Executive Committee will include Drs. Henry Binder, Judy Cho, and Fred Gorelick. ..
  37. Chemical Genetics of Iron Transport
    Marianne Wessling Resnick; Fiscal Year: 2009
    ....
  38. Interdisciplinary Training in Genetics and Complex Disease(RMI)
    Marianne Wessling Resnick; Fiscal Year: 2007
    ..abstract_text> ..
  39. Dynamics of Clathrin Coat Formation in Cells
    Tomas Kirchhausen; Fiscal Year: 2007
    ....
  40. STRUCTURAL CHEMISTRY OF CLATHRIN & ASSOCIATED PROTEINS
    Tomas Kirchhausen; Fiscal Year: 1993
    ..We will modify DNA sequences and transfect cells in an effort to influence aspects of coat formation and function...
  41. EXOCRINE PANCREATIC ZYMOGEN ACTIVATION
    Fred Gorelick; Fiscal Year: 2007
    ....