caveolins

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..These results are consistent with previous immunoelectron microscopic studies demonstrating that dystrophin is localized to plasma membrane caveolae in smooth muscle cells...

..That so many signaling molecules and signaling cascades are regulated by an interaction with the caveolins provides a paradigm by which numerous disease processes may be affected by ablation or mutation of these ..

..Until recently, the only extensively characterised protein components of caveolae were the caveolins. Recently, data from several laboratories have demonstrated that a family of four related proteins, termed ..

..Thus the nature of the lipid anchor on a protein is sufficient to determine submicroscopic localization within the plasma membrane...

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..Our results also suggest that other as yet unknown caveolin family members are likely to exist and may be expressed in a regulated or tissue-specific fashion...

..b>Caveolins 1 and 2 are similar in most respects...

We have studied the biosynthesis and transport of the endogenous caveolins in MDCK cells. We show that in addition to homooligomers of caveolin-1, heterooligomeric complexes of caveolin-1 and -2 are formed in the ER...

..Our results revealed the existence of a virus-activated endocytic pathway from the plasma membrane to the ER that involves neither clathrin nor caveolae and that can be activated also in the presence of cav-1...

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..Overall, these results suggest that caveolin-1 plays multiple roles in the VEGF-induced signaling cascade...

..Our results together suggest that alpha2beta1 integrin clustering defines its own entry pathway that is Pak1 dependent but clathrin and caveolin independent and that is able to sort cargo to caveosomes...

..of VIP21-caveolin as a cholesterol-binding oligomeric protein, and evidence for functional interactions between caveolins and heterotrimeric G proteins...

..b>Caveolins are the principal protein components of caveolae (50-100 nm invaginations found in most cell types) which ..

..Taken together, our data show for the first time a specific role for caveolin-2 in mammalian physiology independent of caveolin-1...

..Consequently, protein-induced membrane domains may affect cell signaling by organizing signal transduction components within the membrane and changing reaction rates...

..Some of these genes may provide novel diagnostic markers for lung cancer...

..This includes brightly fluorescent membrane patches in many cases concentrated at the margin of cells and in arrays. Caveolae may be distinct from other membrane domains due at least in part to caveolin...

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..Thus, caveolin-1 and caveolae play a fundamental role in organizing multiple signaling pathways in the cell...

b>Caveolins are cholesterol-binding proteins involved in the regulation of several intracellular processes, including cholesterol transport...

..Caveolin also influences the formation, morphology, and function of caveolae. The ability of caveolae and caveolin to mediate macromolecular transport directly impacts a variety of physiological and pathophysiological processes...

..The transiently expressed protein appeared on the Golgi-apparatus, the plasma membrane and vesicular structures. We propose that VIP21 is a component of the molecular machinery of vesicular transport...

..The organization and functions of caveolae are mediated by coat proteins (caveolins) and support or adapter proteins (cavins)...

..In addition, the Ras-p42/44 MAPK cascade is hyper-activated. Because a similar premature lactation phenotype is observed in SOCS1 (-/-) null mice, we conclude that caveolin-1 is a novel suppressor of cytokine signaling...

The mammalian caveolin gene family consists of caveolins-1, -2, and -3. The expression of caveolin-3 is muscle-specific...

..They owe their characteristic Omega-shape to complexes of unique proteins, the caveolins, which indirectly tether cholesterol and sphingolipid-enriched membrane microdomains to the cytoskeleton...

..Thus, our results suggest that acylated eNOS may couple muscarinic receptor activation to heart rate control and indicate a key role for eNOS/caveolin interactions in the cholinergic modulation of cardiac myocyte function...

..These results have clear mechanistic implications for understanding the pathogenesis of LGMD-1C at a molecular level...

..They were necessary for formation of caveolae-derived endocytic vesicles and for infection of the cell. Thus, caveolar endocytosis is ligand-triggered and involves extensive rearrangement of the actin cytoskeleton...

..By contrast, upregulation of the caveolin-3 protein is associated with Duchenne muscular dystrophy (DMD). Thus, tight regulation of caveolin-3 appears essential for maintaining normal muscle health and homeostasis...

..These microdomains are referred to as/called caveolae. Caveolins are small proteins (18-24 kDa) that have a hairpin loop conformation with both the N and C termini exposed to ..

..Recent work shows that caveolins can act independently of caveolae, both in cells that lack caveolae (e.g...

..The behavior of cavicles suggests that they function as transport intermediates between caveolae and caveosomes...

..We propose that flotillin-1 is one determinant of a clathrin-independent endocytic pathway in mammalian cells...

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..These findings may have clinical implications for the early diagnosis and treatment of caveolinopathies...

..Thus, caveolae may bud to form discrete carrier vesicles that participate in membrane trafficking...

..We conclude that caveolin-1 can be targeted to a variety of intracellular destinations, which suggests a novel mechanism for the intracellular traffic of this protein...

..within the kinase domain of the EGF-R can mediate the interaction of the EGF-R with the scaffolding domains of caveolins 1 and 3 but not with caveolin 2...

b>Caveolins are important components of caveolae, which have been implicated in vesicular trafficking and signal transduction...

..This study provides several independent lines of evidence supporting the hypothesis that PKCepsilon expression may be sufficient to maintain prostate cancer growth and survival after androgen ablation...

..Taken together, these data indicate that the insulin receptor directly catalyzes the tyrosine phosphorylation of caveolin...

..several recent papers have suggested that caveolae, which are considered to be a specific form of raft, and caveolins, the major membrane proteins of caveolae, are involved in the dynamic cholesterol-dependent regulation of ..

..CCP formation at these sites is much faster than elsewhere on the cell surface, suggesting a virus-induced CCP formation mechanism that may be commonly exploited by many other types of viruses...

..In conclusion, a large fraction of CT can be endocytosed by clathrin-dependent as well as by caveolae- and clathrin-independent endocytosis in different cell types...

..However, since the identification of a family of proteins termed "caveolins", that form and reside in caveolae, a better understanding has emerged...

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..exquisite sensitivity to cholesterol-sequestering agents, can involve caveolae but also exist in cells devoid of caveolins and caveolae...

..These results demonstrate that disruption of the Cav1 gene promotes the progressive steps required for urinary calcium stone formation and establish a new mouse model for urinary stone disease...

..line of immortalized murine neurons (gonadotropin-releasing hormone expressing neurons; GN11), enriched with caveolins and caveolae invaginations that show in vitro chemotaxis upon serum exposure...

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..We speculate that SR-BI is in a more fluid membrane domain that will favor rapid cholesterol flux between the membrane and HDL...

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..However, formation of clusters supporting "hotspots" of Ca(v) activity requires aggregation of macromolecular complexes containing raft components, stabilised by interactions with the cytoskeleton...

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..evidence suggests an important role for membranous invaginations termed caveolae, and their integral protein components caveolins, in the coordination of extracellular contractile stimuli and intracellular effectors in smooth muscle.

..These results provide evidence that caveolin-1 peptide significantly attenuated PMN-induced post-I/R cardiac contractile dysfunction in the isolated perfused rat heart, probably via enhanced release of endothelium-derived NO...

..There is evidence that caveolin-2 and -3 are upregulated during growth and development of articular cartilage, suggesting a role for caveolins in chondrocyte differentiation.

..Direct interactions between resident raft proteins (caveolins and flotillin-1) and insulin-signaling molecules may organize these molecules in space and time to ensure ..

..However, the signal was seen neither on synaptic vesicles nor on presynaptic plasma membranes. CONCLUSIONS. Caveolin-1 is a component protein of synaptic ribbons and may be involved in the regulation of transmitter release...

..Thus, localization of the Cbl-CAP complex to lipid rafts generates a pathway that is crucial in the regulation of glucose uptake...

..These include endothelial cells, macrophages, and smooth muscle cells. Caveolae and caveolin-1 are involved in regulating several signal transduction pathways and processes that play an important role in atherosclerosis...

Caveolae are an abundant feature of mammalian cells. Integral membrane proteins called caveolins drive the formation of caveolae but the precise mechanisms underlying caveola formation, and the origin of caveolae and caveolins during ..

..genetic deletion or overexpression of specific caveolin isoforms has provided key evidence for the importance of caveolins and caveolae in several aspects of the cardiovascular biology, including vascular contractility, lipid ..

..In addition to natural ligands human echovirus-1 (EV1) gains entry into the cell by binding to alpha 2 beta 1 and taking advantage of alpha 2 beta 1 internalization via caveolae...

..In the present study we have analyzed the role of caveolins in intracellular cholesterol cycling using a dominant negative caveolin mutant...

Caveolae and the caveolae coat proteins, caveolins, are putatively implicated in many cellular processes, including transcytosis of macromolecules, cholesterol transport, and signal transduction...

..This is the first in vivo demonstration that caveolin-1 can function as a transformation suppressor gene...

..Therefore, polyomavirus uptake occurs through a class of uncoated vesicles in a clathrin-, caveolin-1-, and dynamin I-independent manner...

..These results suggest that TARP has a role in regulating growth and gene expression in prostate cancer cells...

..b>Caveolins are a class of oligomeric structural proteins that are both necessary and sufficient for caveolae formation...

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..It further implies that G alpha subunits are tethered to Golgi membranes by posttranslational modifications (e.g., palmitoylation) or by binding to another protein(s)...

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..In conclusion, a high level of LDL increases the movement of eNOS to membrane caveolae via the increased stress fibers. The RhoA-mediated pathway may play a crucial role in this process in vascular ECs...

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Caveolae, whose major structural proteins are Caveolins, are classically defined as flask-shaped plasma membrane invaginations with a characteristic diameter of 50 to 100 nm...

..When endogenous caveolins were downregulated, however, the late-phase ERK activation was subsided completely...

..Thus, our results suggest that lipid rafts are sufficient for IGF-1 receptor signaling and the recruitment of signal molecules by caveolin-1 is not essential for IGF-1 receptor signaling...

..Here, more recent data addressing the role of caveolin-1 in cellular signaling and the development of diseases like cancer will be preferentially discussed...

..Furthermore, our findings suggest that caveolae play a pivotal role in lipid raft-dependent LCFA uptake. This transport mechanism is induced in conjunction with cell differentiation and might be mediated by FAT/CD36...

..Our results suggest that Pgp does not interact directly with caveolin-1, and is localized in intermediate-density domains, distinct from classical lipid rafts and caveolae, which can be isolated using Brij-96...

..Thus, ARF6 is involved in the temporal-spatial organization of caveolae/lipid rafts- and ROS-dependent VEGF signaling in ECs as well as in angiogenesis in vivo...

..Our analysis demonstrates that signalling functions such as those controlling cell adhesion, growth and proliferation, are built into the machinery of endocytosis to a much higher degree than previously recognized...

..Thus, the decreased Na(+) absorption induced by AmphoB resulted from reduced cell surface expression of the ENaC, Na(+)K(+)-ATPase pump and NKCC1 and not from direct inhibition of their activities...

..These data suggest a functional link between the localization of CD39 in cholesterol-rich domains of the membrane and its role in thromboregulation...

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..These carriers contained GPI-anchored proteins and fluid phase markers and represented the major vehicles mediating CTB uptake in both WT and caveolae-null cells...

..Thus targeting of caveolae by modifying the capsid of HAdV may represent an alternative approach to enhancing uptake in most hematopoietic cells for future gene therapy...

..These data define a novel molecular mechanism for growth and tumour suppression by caveolin-1...

..We conclude that presenilins are required for caveolae formation by controlling transport of intracellular caveolin 1 to the plasma membrane...

..This suggests that although IGF-IR localizes to caveolin-rich subcellular fractions and coimmunoprecipitates with caveolin, caveolae may not be obligatory for IGF signaling...

..These data support the view that Ca2+ fluxes mediated by Ca2+-ATPase in kidney epithelial cells occur only in caveolae, being strictly dependent on the integrity of these microdomains...

..The PI will introduce anti-sense constructs for Rab3A, Rab3D, caveolins 1, 2, and 3, PKC epsilon, PKC zeta, osteonectin, and adipocyte alpha3 VI collagen...

..We believe that the data obtained from this proposal will provide evidence that even moderate exercise can significantly contribute to brain regeneration in chronic cerebrovascular disorders. ..

..co-crystallization and surface plasmon resonance techniques, to measure interactions with bradykinin receptors, caveolins, dynamin, and nostrin or their respective interactive domains; and 3) to identify and quantify the various ..

..3 channels, 5-HT receptors, c-Src tyrosine kinase and caveolins, all critical regulators of SM function...

..of muscular dystrophy are due to abnormalities of membrane proteins and protein complexes, such as integrins and caveolins, that are known to regulate cellular signaling pathways in general, and cell survival signaling in particular, ..

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..Specific Aim 2: To determine the relevance of 5-HT2A receptor interactions with caveolins-1 and flotillin, which target 5-HT2A receptors...

..There are three caveolin proteins in mammalian cells: caveolins-1, -2, and -3. Caveolins 1 and 2 are usually co-expressed, while caveolin-3 is restricted to muscle cells...

..Specific Aim 2: To determine the relevance of 5-HT2A receptor interactions with caveolins-1 and flotillin, which target 5-HT2A receptors...

..There are three caveolin proteins in mammalian cells: caveolins-1, -2, and -3. Caveolins 1 and 2 are usually co-expressed, while caveolin-3 is restricted to muscle cells...

..These studies will involve the role of caveolins and in mediating regulation of JNK and p381, as well as temporal regulation of the cross-talk mechanisms that ..

..These studies will involve the role of caveolins and in mediating regulation of JNK and p381, as well as temporal regulation of the cross-talk mechanisms that ..

..MDCK and mpkCCD cells and assessment of sumoylation (a novel modification that we have recently identified) of caveolins;2) Functional roles of renal P2Y and adrenergic receptors using receptor-knockout mice...

..MDCK and mpkCCD cells and assessment of sumoylation (a novel modification that we have recently identified) of caveolins; 2) Functional roles of renal P2Y and adrenergic receptors using receptor-knockout mice...

..In this regard, the role of caveolae and caveolins in the lung is an exciting and emerging area of research...

..In this regard, the role of caveolae and caveolins in the lung is an exciting and emerging area of research...

..In this regard, the role of caveolae and caveolins in the lung is an exciting and emerging area of research...

..Recent studies on idiopathic PAH and idiopathic pulmonary fibrosis identified caveolins as important participants in the pathogenesis of both diseases...

..Recent studies on idiopathic PAH and idiopathic pulmonary fibrosis identified caveolins as important participants in the pathogenesis of both diseases...

..localization of Tat to caveolae, the subset of lipid rafts characterized by the presence of proteins termed caveolins. The importance of this observation is related to the fact that a variety of cell surface receptors and ..

..the characterization of a unique insulin-stimulated signaling pathway that leads to tyrosine phosphorylation of caveolins-1 and -2, structural components of specialized cell surface domains termed caveolae...

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..Specific aim 1 is to investigate the dual requirement for caveolins 1 and 2 in the apical targeting of GPI-anchored proteins by Fischer Rat Thyroid (FRT) cells...

..b>Caveolins, proteins abundant in caveolae, provide a scaffold to organize, traffic and regulate signaling molecules...

..b>Caveolins, proteins abundant in caveolae, provide a scaffold to organize, traffic and regulate signaling molecules...

..endothelia vs. fatty stromal cells (mammary adipocytes), in the context of mammary tumorigenesis. ..

..endothelia vs. fatty stromal cells (mammary adipocytes), in the context of mammary tumorigenesis. ..