purinergic receptors

Summary

Summary: Cell surface proteins that bind PURINES with high affinity and trigger intracellular changes which influence the behavior of cells. The best characterized classes of purinergic receptors in mammals are the P1 receptors, which prefer ADENOSINE, and the P2 receptors, which prefer ATP or ADP.

Top Publications

  1. ncbi Inhibition of Ca2+ signaling and glucagon secretion in mouse pancreatic alpha-cells by extracellular ATP and purinergic receptors
    Eva Tudurí
    Institute of Bioengineering, Miguel Hernandez University, Elche, Spain
    Am J Physiol Endocrinol Metab 294:E952-60. 2008
  2. pmc Purinergic receptors contribute to early mesangial cell transformation and renal vessel hypertrophy during angiotensin II-induced hypertension
    Miguel L Graciano
    Department of Physiology and Hypertension and Renal Center of Excellence, Tulane University Health Sciences Center, New Orleans, USA
    Am J Physiol Renal Physiol 294:F161-9. 2008
  3. ncbi Purine-mediated signalling in pain and visceral perception
    G Burnstock
    Autonomic Neuroscience Institute, Royal Free and University College Medical School, Rowland Hill Street, NW3 2PF, London, UK
    Trends Pharmacol Sci 22:182-8. 2001
  4. ncbi Expression of purinergic receptors and modulation of P2X7 function by the inflammatory cytokine IFNgamma in human epithelial cells
    Lynn Welter-Stahl
    Université Paris Denis Diderot, Paris, France
    Biochim Biophys Acta 1788:1176-87. 2009
  5. pmc Activation of purinergic receptors induces proliferation and neuronal differentiation in Swiss Webster mouse olfactory epithelium
    C Jia
    Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI 48823, USA
    Neuroscience 163:120-8. 2009
  6. ncbi Mechanisms of glutamate release from astrocytes: gap junction "hemichannels", purinergic receptors and exocytotic release
    Vladimir Parpura
    Department of Cell Biology and Neuroscience, Center for Nanoscale Science and Engineering, University of California, Riverside, CA 92521, USA
    Neurochem Int 45:259-64. 2004
  7. pmc Neuronal soma-satellite glial cell interactions in sensory ganglia and the participation of purinergic receptors
    Yanping Gu
    Department of Neuroscience and Cell Biology, University of Texas Medical Branch, Galveston, TX 77555 6555, USA
    Neuron Glia Biol 6:53-62. 2010
  8. ncbi Pharmacological properties of purinergic receptors and their effects on proliferation and induction of neuronal differentiation of P19 embryonal carcinoma cells
    R R Resende
    Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Av Prof Lineu Prestes 748, 05508 900 Sao Paulo, SP, Brazil
    Int J Dev Neurosci 26:763-77. 2008
  9. ncbi Purinergic receptors in gastrointestinal inflammation
    Vasantha L Kolachala
    Division of Digestive Diseases, Emory University School of Medicine, Atlanta, GA 30322, USA
    Am J Physiol Gastrointest Liver Physiol 294:G401-10. 2008
  10. ncbi Infection of human endothelium in vitro by cytomegalovirus causes enhanced expression of purinergic receptors: a potential virus escape mechanism?
    Mariët Zandberg
    Department of Internal Medicine, Division of Nephrology, Groningen University Medical Center, Groningen, The Netherlands
    Transplantation 84:1343-7. 2007

Detail Information

Publications223 found, 100 shown here

  1. ncbi Inhibition of Ca2+ signaling and glucagon secretion in mouse pancreatic alpha-cells by extracellular ATP and purinergic receptors
    Eva Tudurí
    Institute of Bioengineering, Miguel Hernandez University, Elche, Spain
    Am J Physiol Endocrinol Metab 294:E952-60. 2008
    ..are particularly sensitive to neural and paracrine factors, in this report we studied the role of purinergic receptors and extracellular ATP, which can be released from nerve terminals and beta-cell secretory granules...
  2. pmc Purinergic receptors contribute to early mesangial cell transformation and renal vessel hypertrophy during angiotensin II-induced hypertension
    Miguel L Graciano
    Department of Physiology and Hypertension and Renal Center of Excellence, Tulane University Health Sciences Center, New Orleans, USA
    Am J Physiol Renal Physiol 294:F161-9. 2008
    ..05). The results suggest that activation of vascular and glomerular purinergic P2 receptors may contribute to the mesangial cell transformation, renal inflammation, and vascular hypertrophy observed in ANG II-dependent hypertension...
  3. ncbi Purine-mediated signalling in pain and visceral perception
    G Burnstock
    Autonomic Neuroscience Institute, Royal Free and University College Medical School, Rowland Hill Street, NW3 2PF, London, UK
    Trends Pharmacol Sci 22:182-8. 2001
    ....
  4. ncbi Expression of purinergic receptors and modulation of P2X7 function by the inflammatory cytokine IFNgamma in human epithelial cells
    Lynn Welter-Stahl
    Université Paris Denis Diderot, Paris, France
    Biochim Biophys Acta 1788:1176-87. 2009
    ..Although a truncated P2X(7) receptor has recently been identified in HeLa cells, the expression of other purinergic receptors or the function of the P2X(7) protein has not been characterized...
  5. pmc Activation of purinergic receptors induces proliferation and neuronal differentiation in Swiss Webster mouse olfactory epithelium
    C Jia
    Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI 48823, USA
    Neuroscience 163:120-8. 2009
    ..We propose that extracellular ATP released upon injury could induce proliferation and promote the neuroregeneration of the olfactory epithelium...
  6. ncbi Mechanisms of glutamate release from astrocytes: gap junction "hemichannels", purinergic receptors and exocytotic release
    Vladimir Parpura
    Department of Cell Biology and Neuroscience, Center for Nanoscale Science and Engineering, University of California, Riverside, CA 92521, USA
    Neurochem Int 45:259-64. 2004
    ..In this review we limit our discussion to findings supporting the exocytotic release of glutamate, as well as two new pathways implicated in this release, the ionotropic (P2X) purinergic receptors and gap junction hemichannels.
  7. pmc Neuronal soma-satellite glial cell interactions in sensory ganglia and the participation of purinergic receptors
    Yanping Gu
    Department of Neuroscience and Cell Biology, University of Texas Medical Branch, Galveston, TX 77555 6555, USA
    Neuron Glia Biol 6:53-62. 2010
    ..The participation of purinergic receptors is emphasized because of their dominant roles in the communication.
  8. ncbi Pharmacological properties of purinergic receptors and their effects on proliferation and induction of neuronal differentiation of P19 embryonal carcinoma cells
    R R Resende
    Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Av Prof Lineu Prestes 748, 05508 900 Sao Paulo, SP, Brazil
    Int J Dev Neurosci 26:763-77. 2008
    ..We have collected evidence for the involvement of metabotropic purinergic receptors in proliferation induction of undifferentiated and neural progenitor cells by using a BrdU-incorporation ..
  9. ncbi Purinergic receptors in gastrointestinal inflammation
    Vasantha L Kolachala
    Division of Digestive Diseases, Emory University School of Medicine, Atlanta, GA 30322, USA
    Am J Physiol Gastrointest Liver Physiol 294:G401-10. 2008
    b>Purinergic receptors comprise a family of transmembrane receptors that are activated by extracellular nucleosides and nucleotides...
  10. ncbi Infection of human endothelium in vitro by cytomegalovirus causes enhanced expression of purinergic receptors: a potential virus escape mechanism?
    Mariët Zandberg
    Department of Internal Medicine, Division of Nephrology, Groningen University Medical Center, Groningen, The Netherlands
    Transplantation 84:1343-7. 2007
    ..Infection with CMV in vivo therefore may result in local increase of adenosine production, providing an anti-inflammatory and antiaggregatory microenvironment, which may facilitate entry of the virus into the target cell...
  11. ncbi Activation of purinergic receptors by ATP inhibits secretion in bovine adrenal chromaffin cells
    A B Harkins
    Department of Neurobiology, Pharmacology and Physiology, The University of Chicago, 947 E 58th Street, Chicago, IL 60637, USA
    Brain Res 885:231-9. 2000
    ..millimolar levels of ATP which is coreleased with catecholamines upon stimulation and the cells express purinergic receptors. We were interested to determine whether autoinhibition produced by ATP binding to purinergic receptors ..
  12. ncbi Extracellular ATP determines 11beta-hydroxysteroid dehydrogenase type 2 activity via purinergic receptors
    Bert Kadereit
    Department of Nephrology Hypertension, University Hospital Berne, Berne, Switzerland
    J Am Soc Nephrol 16:3507-16. 2005
    ..This indicates a bidirectional regulation by nucleotides via purinergic receptors. In JEG-3 cells, ATP/ATPgammaS did not alter 11beta-HSD2 promoter activity but reduced 11beta-HSD2 protein ..
  13. pmc Functional distribution of Ca2+-coupled P2 purinergic receptors among adrenergic and noradrenergic bovine adrenal chromaffin cells
    Angelo R Tomé
    Center for Neurosciences and Cell Biology, University of Coimbra, Coimbra, Portugal
    BMC Neurosci 8:39. 2007
    ..We have taken advantage of the actions of the purinergic agonists ATP and UTP on cytosolic free Ca2+ concentration ([Ca2+]i) to determine whether P2X and P2Y receptors might be asymmetrically distributed among AD and NA chromaffin cells...
  14. ncbi Alteration of muscarinic and purinergic receptors in urinary bladder of rats with cyclophosphamide-induced interstitial cystitis
    Aiko Kageyama
    Department of Pharmacokinetics and Pharmacodynamics and Global Center of Excellence COE Program, School of Pharmaceutical Sciences, University of Shizuoka, 52 1 Yada, Suruga ku, Shizuoka 422 8526, Japan
    Neurosci Lett 436:81-4. 2008
    We characterized muscarnic and purinergic receptors and urodynamic parameters in the bladder of cyclophosphamide (CYP)-treated rats to clarify the mechanisms involved in the pathophysiology of interstitial cystitis (IC)...
  15. pmc Adrenergic receptor activation involves ATP release and feedback through purinergic receptors
    Yuka Sumi
    Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA
    Am J Physiol Cell Physiol 299:C1118-26. 2010
    ..receptor-induced chemotaxis of neutrophils depends on the release of ATP and autocrine feedback through purinergic receptors. Here, we show that adrenergic receptor signaling requires similar purinergic feedback mechanisms...
  16. ncbi Purinergic receptors and pain
    Geoffrey Burnstock
    Autonomic Neuroscience Centre, Royal Free and University College Medical School, Rowland Hill Street, London, UK
    Curr Pharm Des 15:1717-35. 2009
    ..Current developments of compounds for the therapeutic treatment of both visceral and neuropathic pain are discussed...
  17. ncbi Plasma ectonucleotidases prevent desensitization of purinergic receptors in stored platelets: importance for platelet activity during thrombus formation
    Sandra Cauwenberghs
    Department of Biochemistry CARIM, the Sanquin Blood Bank South East, the Department of Health Risk Analysis and Toxicology NUTRIM, Maastricht University, Maastricht, The Netherlands
    Transfusion 46:1018-28. 2006
    Platelets (PLTs) contain purinergic receptors for ATP (P2X1) and ADP (P2Y1 and P2Y12) that rapidly desensitize upon stimulation with these nucleotides...
  18. ncbi The function of microglia through purinergic receptors: neuropathic pain and cytokine release
    Kazuhide Inoue
    Department of Molecular and System Pharmacology, Graduate School of Pharmaceutical Sciences, Kyushu University, Maidashi 3 1 1, Higashi ku, Fukuoka 812 8582, Japan
    Pharmacol Ther 109:210-26. 2006
    ..Thus, diffusible factors released from activated microglia by the stimulation of purinergic receptors may have an important role in the development of neuropathic pain...
  19. pmc Purinergic receptors in airway epithelia
    Eduardo R Lazarowski
    Cystic Fibrosis Pulmonary Research and Treatment Center, Department of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Curr Opin Pharmacol 9:262-7. 2009
    ..Agonist occupation of the P2Y2 receptor promotes inhibition of Na+ absorption as well as CFTR-dependent and CFTR-independent Cl- secretion, ciliary beating, and mucin secretion...
  20. ncbi Painful purinergic receptors
    Diana Donnelly-Roberts
    Abbott Laboratories, R4PM, AP9A 311, 100 Abbott Park Road, Abbott Park, IL 60064, USA
    J Pharmacol Exp Ther 324:409-15. 2008
    ..Thus, ATP acting at multiple purinergic receptors, either directly on neurons (e.g...
  21. doi Purinergic receptors as future targets for treatment of functional GI disorders
    Geoffrey Burnstock
    Autonomic Neuroscience Centre, Royal Free and University College Medical School, Rowland Hill Street, London NW3 2PF, UK
    Gut 57:1193-4. 2008
  22. ncbi Historical review: ATP as a neurotransmitter
    Geoffrey Burnstock
    Autonomic Neuroscience Centre, Royal Free and University College Medical School, Rowland Hill Street, London NW3 2PF, UK
    Trends Pharmacol Sci 27:166-76. 2006
    ..I also discuss the current status of the field, including recent interest in the pathophysiology of purinergic signalling and its therapeutic potential...
  23. ncbi Purinergic receptors involved in the immunomodulatory effects of ATP in human blood
    Els L R Swennen
    Department of Pharmacology and Toxicology, NUTRIM, Maastricht University, P O Box 616, 6200 MD Maastricht, The Netherlands
    Biochem Biophys Res Commun 348:1194-9. 2006
    ....
  24. ncbi Purinergic receptors modulate MAP kinases and transcription factors that control microglial inflammatory gene expression
    Yvonne D Potucek
    Department of Comparative Biosciences and Program in Cellular and Molecular Biology, University of Wisconsin, Madison, WI 53706, United States
    Neurochem Int 49:204-14. 2006
    ..Among the proteins important for modulating the inflammatory function of microglia are the P2 purinergic receptors for which extracellular adenine nucleotides, such as ATP, are ligands...
  25. ncbi Platelet purinergic receptors
    Satya P Kunapuli
    Temple University Medical School, 3420 North Broad Street, Philadelphia, PA 19140, USA
    Curr Opin Pharmacol 3:175-80. 2003
    ..The cloning of the P2Y(12) receptor and its subsequent knockout in mice promises further understanding of its downstream signaling events...
  26. ncbi ATP increases intracellular calcium in supraoptic neurons by activation of both P2X and P2Y purinergic receptors
    Zhilin Song
    Department of Physiology and Biophysics, University of Colorado at Denver and Health Sciences Center, P O Box 6511, Mail Stop 8307, Aurora, CO 80045, USA
    Am J Physiol Regul Integr Comp Physiol 292:R423-31. 2007
    ..Involvement of P2X purinergic receptors (P2XR) in this response was anticipated, because ATP stimulation of vasopressin release from hypothalamo-..
  27. ncbi Purinergic receptors on microglial cells: functional expression in acute brain slices and modulation of microglial activation in vitro
    Clemens Boucsein
    Max Delbruck Center for Molecular Medicine, Cellular Neuroscience, Robert Rössle Strasse10, D 13092, Berlin, Germany
    Eur J Neurosci 17:2267-76. 2003
    ..These findings suggest that microglial cells in situ express different purinergic receptors with distinct sensitivity and functional coupling...
  28. ncbi Purinergic receptors are part of a functional signaling system for proliferation and differentiation of human epidermal keratinocytes
    Aina V H Greig
    Autonomic Neuroscience Institute, Royal Free and University College Medical School, Royal Free Campus, Rowland Hill Street, London, UK
    J Invest Dermatol 120:1007-15. 2003
    ..01) and 1000 microm ATP (p<0.001) on cell number. These results imply that different purinergic receptors have different functional roles in the human epidermis with P2Y1 and P2Y2 receptors controlling ..
  29. ncbi Purinergic receptors: their role in nociception and primary afferent neurotransmission
    G Burnstock
    Department of Anatomy and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK
    Curr Opin Neurobiol 6:526-32. 1996
    ....
  30. ncbi Purinergic signalling--an overview
    Geoffrey Burnstock
    Autonomic Neuroscience Centre, Royal Free and University College Medical School, London, UK
    Novartis Found Symp 276:26-48; discussion 48-57, 275-81. 2006
    ..Finally the purinoceptor subtypes expressed on astrocytes, oligodendrocytes, Schwann cells, microglia, Müller cells and enteric glial cells will be summarized as well as evidence for non-lytic release of ATP from glial cells...
  31. pmc Mechanisms of excitatory neuromuscular transmission in the guinea-pig urinary bladder
    H Hashitani
    Department of Zoology, The University of Melbourne, Parkville, Victoria 3052, Australia
    J Physiol 524:565-79. 2000
    ....
  32. ncbi Differential activation of subtype purinergic receptors modulates Ca(2+) mobilization and COX-2 in human microglia
    Hyun B Choi
    Department of Pharmacology and Therapeutics, Faculty of Medicine, University of British Columbia, Vancouver, British Columbia, Canada
    Glia 43:95-103. 2003
    We have studied modulation of purinergic receptors (P(2Y) and P(2X) subtypes) on changes in intracellular Ca(2+) [Ca(2+)](i) and expression and production of COX-2 in human microglia...
  33. ncbi Mechanical lesion activates newly identified NFATc1 in primary astrocytes: implication of ATP and purinergic receptors
    José M Pérez-Ortiz
    Department of Medical Sciences, University of Castilla La Mancha, Av Almansa 14, Albacete 02006, Spain
    Eur J Neurosci 27:2453-65. 2008
    ..modulators showed that such ATP action is mediated by stimulation of several G(q)-protein-coupled P2Y purinergic receptors, among which P2Y(1) and P2Y(6) are included...
  34. ncbi Autocrine/paracrine stimulation of purinergic receptors in osteoblasts: contribution of vesicular ATP release
    Milena Romanello
    Department of Biochemistry, University of Trieste, via L Giorgeri 1, 34100 Trieste, Italy
    Biochem Biophys Res Commun 331:1429-38. 2005
    ..Finally, elevated levels of cytosolic Ca2+ activated massive ATP release and a dose-dependent loss of quinacrine granules. The contribution of a vesicular mechanism for ATP release is proposed to sustain paracrine osteoblast activation...
  35. ncbi Expression of functional purinergic receptors in pulmonary neuroepithelial bodies and their role in hypoxia chemotransmission
    Xiao Wen Fu
    Division of Pathology, Department of Pediatric Laboratory Medicine, The Research Institute, The Hospital for Sick Children and University of Toronto, Toronto, Ontario M5G 1X8, Canada
    Biol Chem 385:275-84. 2004
    ..These data suggest that functional P2X2/3 heteromeric receptors are expressed in NEB cells. The possible function of these purinoreceptors in NEB cells could include modulation of hypoxia chemotransmission...
  36. ncbi Membrane-specific expression of functional purinergic receptors in normal human nasal epithelial cells
    Chang Hoon Kim
    Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, 120 752, Korea
    Am J Physiol Lung Cell Mol Physiol 287:L835-42. 2004
    ..In conclusion, the NHNE cells express functionally active P2Y2, P2Y6, and P2Y11 receptors in a membrane-specific pattern, which may play an important role in the control of mucin and fluid secretion in NHNE cells...
  37. ncbi Loss of muscarinic and purinergic receptors in urinary bladder of rats with hydrochloric acid-induced cystitis
    Akira Yoshida
    Department of Pharmacokinetics and Pharmacodynamics and Global Center of Excellence Program, University of Shizuoka School of Pharmaceutical Sciences, Shizuoka, Japan
    Urology 76:1017.e7-12. 2010
    ....
  38. ncbi Activation of purinergic receptors by ATP induces ventricular tachycardia by membrane depolarization and modifications of Ca2+ homeostasis
    Iman S Gurung
    Department of Biochemistry, Hopkins Building University of Cambridge, Tennis Court Road, Cambridge, CB2 1QW, UK
    J Mol Cell Cardiol 47:622-33. 2009
    ..In conclusion, extracellular ATP activates purinergic receptors and induces arrhythmic activity through modifications of Ca2+ homeostasis and an activation of depolarizing ..
  39. pmc Functional interaction between native G protein-coupled purinergic receptors in Xenopus follicles
    Rogelio O Arellano
    Departamento de Neurobiologia Celular y Molecular, Instituto de Neurobiologia, Universidad Nacional Autonoma de Mexico, Boulevard Juriquilla 3001, Juriquilla Querétaro, Queretaro, Mexico
    Proc Natl Acad Sci U S A 106:16680-5. 2009
    b>Purinergic receptors are expressed in the membrane of the follicular cell layer that communicates with the Xenopus oocyte...
  40. ncbi Evoked and spontaneous purinergic junctional Ca2+ transients (jCaTs) in rat small arteries
    Christine Lamont
    Department of Physiology, School of Medicine, University of Maryland, Baltimore, MD 21201, USA
    Circ Res 91:454-6. 2002
    ..Visualization of sympathetic neurotransmission shows that purinergic components dominate at low frequencies of sympathetic nerve fiber activation...
  41. ncbi Ionic currents activated via purinergic receptors in the cumulus cell-enclosed mouse oocyte
    Rogelio O Arellano
    Centro de Neurobiologia, Universidad Nacional Autonoma de Mexico, Queretaro 76230, Mexico
    Biol Reprod 67:837-46. 2002
    ..Both the ion channels and the receptors seemed to be located in the cumulus cells, which transmit their corresponding electrical signals to the oocyte via gap junction channels...
  42. ncbi Nonselective cation conductance activated by muscarinic and purinergic receptors in rat spiral ganglion neurons
    Ken Ito
    Laboratoire de Biologie Cellulaire et Moleculaire de l Audition, Institut National de la Santé et de la Recherche Médicale EMI 99 27, Universite de Bordeaux 2, Hopital Pellegrin, 33076 Bordeaux, France
    Am J Physiol Cell Physiol 282:C1121-35. 2002
    ....
  43. ncbi Inhibitory influences of xanthine oxidase inhibitor and angiotensin I-converting enzyme inhibitor on multinucleated giant cell formation from monocytes by downregulation of adhesion molecules and purinergic receptors
    K Mizuno
    Department of Dermatology, Kansai Medical University, 10 15 Fumizono, Moriguchi, Osaka 570 8507, Japan
    Br J Dermatol 150:205-10. 2004
    ..There have been reported cases showing that these two agents are effective for the treatment of granulomatous diseases such as sarcoidosis, although the mode of action is not elucidated...
  44. ncbi Detection of P2X purinergic receptors on human B lymphocytes
    R Sluyter
    Department of Medicine, University of Sydney, Nepean Hospital, Penrith, NSW, Australia
    Cell Tissue Res 304:231-6. 2001
    ..We observed that P2X1, P2X2, P2X4 and P2X7 subtypes, but not P2X3, P2X5 and P2X6 subtypes, are present on B lymphocytes...
  45. pmc Adenosine receptors on human airway epithelia and their relationship to chloride secretion
    E R Lazarowski
    Department of Medicine, University of North Carolina, Chapel Hill 27599
    Br J Pharmacol 106:774-82. 1992
    ..Activation of this receptor might lead to stimulation of Cl- secretion in amiloride pretreated normal but not CF cells...
  46. ncbi Stimulation of purinergic receptors modulates chemokine expression in human keratinocytes
    Saveria Pastore
    Laboratory of Tissue Engineering and Cutaneous Physiopathology, Istituto Dermopatico dell Immacolata, IRCCS, Roma, Italy
    J Invest Dermatol 127:660-7. 2007
    ..Collectively, our data suggest that the orchestrated activation of distinct P2Y and P2X receptors modulates skin inflammation...
  47. ncbi Expression of purinergic receptors in non-melanoma skin cancers and their functional roles in A431 cells
    Aina V H Greig
    Autonomic Neuroscience Institute, Royal Free and University College Medical School, Royal Free Campus, London, UK
    J Invest Dermatol 121:315-27. 2003
    We investigated the use of purinergic receptors as a new treatment modality for nonmelanoma skin cancers. Purinergic receptors, which bind adenosine 5'-tri-phosphate, are expressed on human cutaneous keratinocytes...
  48. ncbi Single-cell fura-2 microfluorometry reveals different purinoceptor subtypes coupled to Ca2+ influx and intracellular Ca2+ release in bovine adrenal chromaffin and endothelial cells
    E Castro
    Department of Biochemistry, University of Coimbra, Portugal
    Pflugers Arch 426:524-33. 1994
    ..An intracellular Ca(2+)-releasing P2U-type purinoceptor is specifically localized to adrenal endothelial cells, while a subpopulation of chromaffin cells expresses a non-P2X, non-P2Y subtype exclusively coupled to Ca2+ influx...
  49. ncbi Modulation of feeding behaviour by blocking purinergic receptors in the rat nucleus accumbens: a combined microdialysis, electroencephalographic and behavioural study
    H Kittner
    Rudolf Boehm Institute of Pharmacology and Toxicology, University of Leipzig, Härtelstrasse 16 18, D 04107 Leipzig, Germany
    Eur J Neurosci 19:396-404. 2004
    ....
  50. ncbi Activation of p90RSK and growth stimulation of multicellular tumor spheroids are dependent on reactive oxygen species generated after purinergic receptor stimulation by ATP
    H Sauer
    Department of Neurophysiology, University of Cologne, D-50931 Cologne, Germany
    FASEB J 15:2539-41. 2001
    ..Our data indicate that p90RSK downstream of ERK1/2 is the molecular target for ROS generated through stimulation of purinergic receptors by ATP.
  51. ncbi Leukotriene and purinergic receptors are involved in the hyperpolarizing effect of glucagon in liver cells
    Lisa Fischer
    Institute of Veterinary Physiology, Vetsuisse Faculty University of Zurich, Winterthurerstrasse 260, 8057 Zurich, Switzerland
    Biochim Biophys Acta 1669:26-33. 2005
    ..These findings suggest that glucagon affects the hepatic membrane potential partly by inducing the formation and release of leukotrienes and release of ATP acting on purinergic receptors of the liver cell membrane.
  52. ncbi Differential regulations between adenosine triphosphate (ATP)- and uridine triphosphate-induced Cl(-) secretion in bovine tracheal epithelium. Direct stimulation of P1-like receptor by ATP
    S Kanoh
    Third Department of Medicine, National Defense Medical College, Saitama, Japan
    Am J Respir Cell Mol Biol 25:370-6. 2001
    ..Therefore, ATP stimulates glybenclamide-sensitive Cl(-) secretion, and this action is partly mediated by PKA-dependent pathway via P1-like receptor...
  53. ncbi Mechanisms underlying extracellular ATP-evoked interleukin-6 release in mouse microglial cell line, MG-5
    Y Shigemoto-Mogami
    Division of Pharmacology, National Institute of Health Sciences, Setagaya, Tokyo, Japan
    J Neurochem 78:1339-49. 2001
    ..This is a novel pathway for the induction of cytokines in microglia...
  54. pmc Anandamide acts as an intracellular messenger amplifying Ca2+ influx via TRPV1 channels
    Mario Van Der Stelt
    Endocannabinoid Research Group, Institute of Biomolecular Chemistry, Consiglio Nazionale delle Ricerche, Pozzuoli NA, Italy
    EMBO J 24:3026-37. 2005
    ..This novel intracellular function of anandamide may precede its action at cannabinoid receptors, and might be relevant to its control over neurotransmitter release...
  55. pmc Adenosine 5'-triphosphate (ATP) is an excitatory cotransmitter with noradrenaline to the smooth muscle of the rat prostate gland
    Sabatino Ventura
    Department of Pharmaceutical Biology and Pharmacology, Victorian College of Pharmacy, Monash University, 381 Royal Parade, Parkville, Victoria 3052, Australia
    Br J Pharmacol 138:1277-84. 2003
    ....
  56. ncbi Nitrergic prejunctional inhibition of purinergic neuromuscular transmission in the hamster proximal colon
    Hayato Matsuyama
    Department of Pathogenetic Veterinary Science, The United Graduate School, Gifu University, Yanagido 1 1, Japan
    J Neurophysiol 89:2346-53. 2003
    ..In addition, VIP may be involved in the neurotransmitter in the hamster proximal colon...
  57. ncbi Spatial and temporal aspects of Ca2+ signaling mediated by P2Y receptors in cultured rat hippocampal astrocytes
    Schuichi Koizumi
    Division of Pharmacology, National Institute of Health Sciences, 1 18 1 Kamiyoga, Setagaya, 158, Tokyo, Japan
    Life Sci 72:431-42. 2002
    ..It should be noted that these Ca2+ signals and their modulation by Zn2+ could occur in the hippocampus in situ since both ATP and Zn2+ are rich in the hippocampus and could be released by excitatory stimulation...
  58. ncbi Pharmacology of a unique adenosine binding site in rat brain using a selective ligand
    K Yoshioka
    Department of Molecular and Cellular Neurobiology, Tokyo Metropolitan Institute for Neuroscience, Tokyo, Japan
    Clin Exp Pharmacol Physiol 28:278-84. 2001
    ..5. These studies reveal that the [3H]-NECA binding assay in combination with HAK2701 is successful in the characterization of P3LP, especially the membrane-bound form...
  59. pmc Intracellular pathways regulating ciliary beating of rat brain ependymal cells
    T Nguyen
    Departments of Bioengineering, Physiology and Biophysics and Medicine, University of Washington, Box 357962, Seattle, Washington 98195, USA
    J Physiol 531:131-40. 2001
    ..6. The opposite response of ciliated ependymal cells to 5-HT and ATP provides a novel mechanism for their active involvement in central nervous system signalling...
  60. ncbi Beta,gamma-methylene ATP-induced cAMP formation in C6Bu-1 cells: involvement of local metabolism and subsequent stimulation of adenosine A2B receptor
    S Ohkubo
    Department of Pharmacology, School of Medicine, Fukushima Medical University, Fukushima, Japan
    J Neurochem 76:872-80. 2001
    ..These results suggest that C6Bu-1 cells possess ecto-enzymes converting beta,gamma-MeATP to adenosine, and the locally accumulated adenosine in this mechanism efficiently stimulates A2B receptors in a manner resistant to exogenous ADA...
  61. ncbi Schwann cells in rat vascular autonomic nerves activated via purinergic receptors
    Yong Qi Lin
    The Neurobiology Laboratory, Department of Physiology, University of Sydney, NSW, Australia
    Neuroreport 17:531-5. 2006
    ..Recently, purinergic receptors have been shown to exist on Schwann cells of axon varicosities in visceral smooth muscle where they are ..
  62. ncbi Ca2+ activates cystic fibrosis transmembrane conductance regulator- and Cl- -dependent HCO3 transport in pancreatic duct cells
    Wan Namkung
    Department of Pharmacology and Brain Korea 21 Project for Medical Sciences and Department of Physiology, Yonsei University College of Medicine, Seoul 120 752, Korea
    J Biol Chem 278:200-7. 2003
    ..These results provide a molecular basis for calcium-induced bicarbonate secretion in pancreatic duct cells and highlight the importance of CFTR in epithelial bicarbonate secretion induced by various stimuli...
  63. pmc Glial cells dilate and constrict blood vessels: a mechanism of neurovascular coupling
    Monica R Metea
    Department of Neuroscience, University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Neurosci 26:2862-70. 2006
    ..These results indicate that glial cells contribute to neurovascular coupling and suggest that regulation of blood flow may involve both vasodilating and vasoconstricting components...
  64. ncbi Adenine nucleotide-induced activation of adenosine A(2B) receptors expressed in Xenopus laevis oocytes: involvement of a rapid and localized adenosine formation by ectonucleotidases
    Isao Matsuoka
    Department of Pharmacology, School of Medicine, Fukushima Medical University, Fukushima, Japan
    Mol Pharmacol 61:606-13. 2002
    ..These results support our hypothesis that adenine nucleotides are rapidly and locally converted into Ade on the membrane surface, resulting in the activation of A(2B) receptors...
  65. ncbi Resetting intrinsic purinergic modulation of neural activity: an associative mechanism?
    Nicholas Dale
    Department of Biological Sciences, University of Warwick, Coventry, CV4 7AL, United Kingdom
    J Neurosci 22:10461-9. 2002
    ..The length of the time window and the magnitude of the prolongation of neural activity both depend on the strength of the feedforward ADP-mediated inhibition of the ecto-5'-nucleotidase...
  66. ncbi Expression of a P2X7 receptor by a subpopulation of human osteoblasts
    A Gartland
    Department of Human Anatomy and Cell Biology, The University of Liverpool, United Kingdom
    J Bone Miner Res 16:846-56. 2001
    ..These data show for the first time the expression of functional P2X7 receptors in a subpopulation of osteoblasts, activation of which can result in ATP-mediated apoptosis...
  67. ncbi ATP induces leukemia inhibitory factor mRNA in cultured rat astrocytes
    Hisashi Yamakuni
    Department of Molecular Pharmacology, Faculty of Pharmaceutical Sciences, Kyoto University, 606 8501, Kyoto, Japan
    J Neuroimmunol 129:43-50. 2002
    ..Experiments using ATP, UTP and related compounds showed the involvement of P2Y2 and P2Y4 purinoceptors in the expression induced by ATP...
  68. ncbi The DC electrical-field-induced Ca(2+) response and growth stimulation of multicellular tumor spheroids are mediated by ATP release and purinergic receptor stimulation
    Heinrich Sauer
    Department of Neurophysiology, University of Cologne, Robert Koch Strasse 39, D 50931 Cologne, Germany
    J Cell Sci 115:3265-73. 2002
    ..that electrical field treatment of multicellular tumor spheroids results in ATP release, which concomitantly activates purinergic receptors, elicits a Ca(2+) wave spreading through the tumor spheroid tissue and stimulates tumor growth.
  69. ncbi Purinergic receptors have different effects in rat exocrine pancreas. Calcium signals monitored by fura-2 using confocal microscopy
    Ivana Novak
    August Krogh Institute, University of Copenhagen, Denmark
    Cell Physiol Biochem 12:83-92. 2002
    ..The low number of functional P2 receptors in acini might be related to the fact that they release ATP. Thereby acini would avoid autocrine stimulation and initiation of autodigestive processes, such as occurs in pancreatitis...
  70. ncbi Ontogeny of purinergic receptor-regulated Ca2+ signaling in mouse cortical collecting duct epithelium
    Johannes Tschop
    Physiologisches Institut, Ludwig Maximilians Universitat, Munich, Germany
    Cell Physiol Biochem 12:75-82. 2002
    ..This pathway might by mediated by the increasing expression of P2X(4)-receptors resulting in an increasing ATP-dependent membrane depolarization and activation of L-type Ca2+ channels...
  71. ncbi Purinergic receptor signaling at the basolateral membrane of macula densa cells
    Ruisheng Liu
    Department of Physiology, Uppsala University, Uppsala, Sweden
    J Am Soc Nephrol 13:1145-51. 2002
    b>Purinergic receptors are important in the regulation of renal hemodynamics; therefore, this study sought to determine if such receptors influence macula densa cell function...
  72. ncbi ATPgammaS enhances the production of inflammatory mediators by a human dermal endothelial cell line via purinergic receptor signaling
    Kristina Seiffert
    Department of Dermatology, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021 USA
    J Invest Dermatol 126:1017-27. 2006
    ..For example, ATP may contribute to both the vasodilation and the inflammation associated with rosacea...
  73. ncbi Multiple purinergic receptors lead to intracellular calcium increases in cultured rat Sertoli cells
    W H Ko
    Department of Physiology, Faculty of Medicine, The Chinese University of Hong Kong, Basic Medical Sciences Building, Shatin, Hong Kong, China
    Life Sci 72:1519-35. 2003
    ..Taken together, these data indicate that both metabotropic receptors and ionotropic receptors are present in rat Sertoli cells through which extracellular nucleotides can act...
  74. ncbi Calcium signaling to nucleus via store-operated system during cell cycle in retinal neuroepithelium
    Miho Sugioka
    Department of Physiology I, Nara Medical University, Shijo cho 840, Kashihara 634 8521, Japan
    Neurosci Res 45:447-58. 2003
    ..These results suggest that the Ca(2+) signaling to nucleus via the store-operated system including the ERs in the outer process is crucial for the cell cycle progression in the retinal neuroepithelium...
  75. ncbi Potential therapeutic targets in the rapidly expanding field of purinergic signalling
    G Burnstock
    Autonomic Neuroscience Institute, Royal Free and University College Medical School, London
    Clin Med 2:45-53. 2002
    ....
  76. ncbi Changes in the control of gastric motor activity during metamorphosis in the amphibian Xenopus laevis, with special emphasis on purinergic mechanisms
    Monika Sundqvist
    Department of Zoophysiology, Goteborg University, SE 405 30 Goteborg, Sweden
    J Exp Biol 211:1270-80. 2008
    ..An adenosine A(1)-like receptor mediating relaxation and a P2X-like receptor mediating contraction is demonstrated at all stages...
  77. ncbi Pregnancy-enhanced Ca2+ responses to ATP in uterine artery endothelial cells is due to greater capacitative Ca2+ entry rather than altered receptor coupling
    Shannon M Gifford
    Perinatal Research Laboratories, Department of Obstetrics and Gynecology, University of Wisconsin Madison, 7E Meriter Hospital Park, 202 South Park Street, Madison, Wisconsin 53715, USA
    J Endocrinol 190:373-84. 2006
    ..We conclude that changes at the level of capacitative entry mechanisms rather than switching of receptor subtype or coupling to phospholipase C underlies pregnancy adaptation of UAEC at the level of Ca(2+)signaling...
  78. pmc Activation of purinergic receptor subtypes modulates odor sensitivity
    Colleen C Hegg
    Department of Physiology, University of Utah, Salt Lake City, Utah 84108 1297, USA
    J Neurosci 23:8291-301. 2003
    ..Here we show that multiple subtypes of purinergic receptors are differentially expressed in olfactory receptor neurons and sustentacular support cells...
  79. pmc Astrocyte calcium waves: what they are and what they do
    Eliana Scemes
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Glia 54:716-25. 2006
    ....
  80. ncbi Differentiation-dependent alterations in the extracellular ATP-evoked calcium fluxes of cultured skeletal muscle cells from mice
    Gyula Péter Szigeti
    Department of Physiology, Medical and Health Science Centre, University of Debrecen, P O Box 22, 98 Nagyerdei krt, Debrecen, 4012, Hungary
    Pflugers Arch 453:509-18. 2007
    ..The concentration dependence of I (ATP) can be fitted using a Hill equation, yielding an EC(50) of 56 microM. Results are consistent with the parallel activation of both P2X and P2Y receptors...
  81. ncbi Potentiation of ATP-induced Ca2+ mobilisation in human retinal pigment epithelial cells
    David J Collison
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
    Exp Eye Res 80:465-75. 2005
    ....
  82. pmc Inhibition by Zn2+ of uridine 5'-triphosphate-induced Ca(2+)-influx but not Ca(2+)-mobilization in rat phaeochromocytoma cells
    S Koizumi
    Division of Pharmacology, National Institute of Health Sciences, Tokyo, Japan
    Br J Pharmacol 115:1502-8. 1995
    ..The dopamine release evoked by UTP requires external Ca2+ which may enter the cells through pathways sensitive to Zn2+ but insensitive to Cd2+ or nicardipine...
  83. ncbi Multiple actions of extracellular ATP and adenosine on calcium currents mediated by various purinoceptors in neurons of nucleus tractus solitarius
    Yoshihito Aoki
    Department of Physiology, Tokyo Dental College, 1 2 2 Masago, Mihama ku, Chiba 261 8502, Japan
    Neurosci Res 50:245-55. 2004
    ..Activation of A(1) and/or A(2) receptors inhibit N- and P/Q-types VDCCs via G(i)-protein betagamma subunits. Activation of P2X purinoceptors facilitates Ca(2+) entry in NTS...
  84. pmc Intracellular cross-talk between receptors coupled to phospholipase C via pertussis toxin-sensitive and insensitive G-proteins in DDT1MF-2 cells
    J M Dickenson
    Department of Physiology and Pharmacology, Medical School, Queen s Medical Centre, Nottingham
    Br J Pharmacol 109:719-24. 1993
    ..In contrast,activation of ATP-receptors which are linked to PTX-insensitive G-protein(s) do not interact synergically with histamine H1-receptors...
  85. ncbi EGF activates intracellular and intercellular calcium signaling by distinct pathways in tumor cells
    Joshua A Bryant
    Department of Neurology David Geffen School of Medicine at University of California Los Angeles, Los Angeles, California90095, USA
    Cancer Biol Ther 3:1243-9. 2004
    ..of intercellular Ca2+ waves, which have been previously shown to involve release of ATP and activation of purinergic receptors. Cells transiently exposed to EGF also showed a sustained potentiation of the Ca2+ signaling response to ..
  86. ncbi Regulation of ion transport via apical purinergic receptors in intact rabbit airway epithelium
    Asser Nyander Poulsen
    Department of Medical Physiology, The Panum Institute, University of Copenhagen, Blegdamsvej 3, 2200, Copenhagen N, Denmark
    Pflugers Arch 450:227-35. 2005
    We investigated purinergic receptors involved in ion transport regulation in the intact rabbit nasal airway epithelium...
  87. ncbi Cell biology. Purinergic chemotaxis
    Joel Linden
    Department of Medicine, University of Virginia, Charlottesville, VA 22908, USA
    Science 314:1689-90. 2006
  88. pmc Action of purine and pyrimidine nucleotides on the rat superior cervical ganglion
    G P Connolly
    Department of Physiology, University College of London
    Br J Pharmacol 110:1297-304. 1993
    ..There is also some evidence to suggest the presence of receptors for UTP, i.e., pyrimidinoceptors, which depolarize SCG neurones...
  89. ncbi Purinergic actions on neurons that modulate nociception in the rostral ventromedial medulla
    N R Selden
    Department of Neurological Surgery, Oregon Health and Science University, Mail code CH8N, 3303 Southwest Bond Avenue, Portland, OR 97239, USA
    Neuroscience 146:1808-16. 2007
    ..Immunohistochemical localization of purinergic receptors confirms the presence of some subtypes of P2X, P2Y and A1 receptors on neuronal cell bodies and fibers ..
  90. pmc An electrophysiological study of excitatory purinergic neuromuscular transmission in longitudinal smooth muscle of chicken anterior mesenteric artery
    Maisa Khalifa
    Department of Basic Veterinary Science, Laboratory of Physiology, The United Graduate School, Gifu University, 1 1 Yanagido, Gifu, 501 1193, Japan
    Br J Pharmacol 144:830-9. 2005
    ....
  91. pmc Heterologous desensitization of the sphingosine-1-phosphate receptors by purinoceptor activation in renal mesangial cells
    Cuiyan Xin
    Pharmazentrum Frankfurt, Klinikum der Johann Wolfgang Goethe Universitat, Theodor Stern Kai 7, D 60590 Frankfurt am Main, Germany
    Br J Pharmacol 143:581-9. 2004
    ....
  92. ncbi Possible involvement of ATP-purinoceptor signalling in the intercellular synchronization of intracellular Ca2+ oscillation in cultured cardiac myocytes
    Yukako Nakayama
    Laboratory of Cellular Cybernetics, Graduate School of Information Science and Technology, Hokkaido University, Sapporo 060 0814, Japan
    Biosystems 90:179-87. 2007
    ..The present study suggested that the extracellular ATP-purinoceptor system was responsible for the intercellular synchronization of Ca(2+) oscillation among cardiac myocytes...
  93. ncbi ATP causes glomus cell [Ca2+]c increase without corresponding increases in CSN activity
    A Mokashi
    Department of Physiology, School of Medicine, University of Pennsylvania, B400 Richards Building 37th and Hamilton Walk, Philadelphia, PA 19104 6085, USA
    Respir Physiol Neurobiol 138:1-18. 2003
    ..Thus, ATP dependent linear increases in [Ca(2+)](c) did not give rise to a corresponding increase in CSN activities, contravening the normally expected increase in CSN activities following [Ca(2+)](c) rise...
  94. pmc A quantitative model of purinergic junctional transmission of calcium waves in astrocyte networks
    M R Bennett
    The Neurobiology Laboratory, Institute for Biomedical Research, Department of Physiology, University of Sydney, New South Wales, Australia
    Biophys J 89:2235-50. 2005
    ..The properties of purinergic transmission at astrocyte junctions may determine many of the characteristics of Ca2+ propagation in networks of these cells...
  95. pmc Calcium increases in retinal glial cells evoked by light-induced neuronal activity
    Eric A Newman
    Department of Neuroscience, University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Neurosci 25:5502-10. 2005
    ....
  96. ncbi Uridine-5'-triphosphate and adenosine triphosphate gammaS induce mucin secretion via Ca2+-dependent pathways in human nasal epithelial cells
    Jae Young Choi
    Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, South Korea
    Acta Otolaryngol 123:1080-6. 2003
    ..We also examined the effect of UTP (an agonist for P2Y2) and ATPgammaS (an agonist for P2Y11) on mucin secretion and mucin gene expression...
  97. ncbi Involvement of peripheral purinoceptors in sympathetic modulation of capsaicin-induced sensitization of primary afferent fibers
    Yong Ren
    Department of Neuroscience and Cell Biology, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77555 1069, USA
    J Neurophysiol 96:2207-16. 2006
    ..Combined with our previous study, our data also provide further evidence that the sensitization of primary afferent nociceptors is subject to sympathetic modulation by activation of P2X as well as alpha(1)-adrenergic receptors...
  98. ncbi The ATP-gated P2X1 receptor plays a pivotal role in activation of aspirin-treated platelets by thrombin and epinephrine
    Magnus Grenegård
    Department of Medicine and Health, Division of Drug Research, Division of Clinical Chemistry, Cardiovascular Inflammation Research Center, Linkoping University, Linköping SE 581 85 Sweden
    J Biol Chem 283:18493-504. 2008
    ..The roles of released ATP and ADP were elucidated by using antagonists of the purinergic receptors P2X(1), P2Y(1), and P2Y(12) (i.e. NF449, MRS2159, MRS2179, and cangrelor)...
  99. ncbi The effect of a hyposmotic shock and purinergic agonists on K+(Rb+) efflux from cultured human breast cancer cells
    I F Gow
    Hannah Research Institute, Ayr, Scotland, UK
    Biochim Biophys Acta 1712:52-61. 2005
    ..It appears that the hyposmotically-activated and ATP-stimulated K(+) efflux pathways are separate entities. However, there may be a degree of 'crosstalk' between the two pathways...
  100. ncbi ATP- and adenosine-mediated signaling in the central nervous system: adenosine receptor activation by ATP through rapid and localized generation of adenosine by ecto-nucleotidases
    Isao Matsuoka
    Department of Pharmacology, Fukushima Medical University School of Medicine, Japan
    J Pharmacol Sci 94:95-9. 2004
    ....
  101. pmc Adenine nucleotide changes in the remnant liver: An early signal for regeneration after partial hepatectomy
    Sara Crumm
    Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, PA, USA
    Hepatology 48:898-908. 2008
    ..Pretreatment with the purinergic receptor antagonist suramin also partly suppressed early regenerative signals and c-fos expression, but without preventing TAN loss...

Research Grants62

  1. Regulation of IFN-beta induction by P2X7 purinergic receptor signaling
    JUDITH ANNE SMITH; Fiscal Year: 2011
    ..tissue damage leads to sufficient release of extracellular purine nucleotides to stimulate immune cells via purinergic receptors such as P2X7...
  2. Astrocytic Ca2+ Signaling in the Ischemic Penumbra
    Maiken Nedergaard; Fiscal Year: 2011
    ..Besides its activation of local purinergic receptors, it is also rapidly converted to adenosine, which potently inhibits excitatory transmission while lowering ..
  3. Role of Integrins in Bladder Urothelium
    Warren G Hill; Fiscal Year: 2013
    ..been show to be an extremely important autocrine and paracrine mediator in the bladder and signals through purinergic receptors on both the luminal surface and in suburothelial layers e.g. on afferent nerve fibers...
  4. Purinergic signaling in sleep apnea
    Elzbieta Kaczmarek; Fiscal Year: 2012
    ..We have recent evidence that extracellular nucleotides, such as ATP, ADP and UTP, acting via specific P2 purinergic receptors, decrease high glucose- induced ROS formation, attenuate inflammatory effects of tumor necrosis factor ..
  5. Mechanism and functional role of AKAP79/150 in M current control
    Mark S Shapiro; Fiscal Year: 2012
    ..that are critical to pain and inflammation, including bradykinin, PAR-2, histamine, angiotensin II and purinergic receptors. We will determine which Gq/11-coupled receptors modulate NG neurons, and the role of AKAP79/150 in ..
  6. DEPOLARIZATION-SECRETION COUPLING IN NERVE TERMINALS
    Jose R Lemos; Fiscal Year: 2012
    ..takes advantages of newly available genetic tools that facilitate the elucidation of the function of these purinergic receptors with greater specificity than is possible with traditional antagonist drugs...
  7. The Three Rs of Immunity: Recognition, Response and Resolution
    Robert A Clark; Fiscal Year: 2010
    ..in shock / trauma / sepsis, genetics of immunodeficiency, innate immune recognition of microbial products, purinergic receptors and immunity, soluble pattern recognition receptors, regulatory myeloid cells, exosomes in immunity, ..
  8. Novel transgenic reporter/deleter allele for Type I taste cells
    Sue Kinnamon; Fiscal Year: 2013
    ..They release ATP to activate purinergic receptors on afferent nerve fibers...
  9. Intercellular networks and luminal acidification in the male reproductive tract
    Sylvie Breton; Fiscal Year: 2013
    ..Aim 1B will identify and characterize the apical purinergic receptors (e.g. A2B, P2X4), and bicarbonate transporters (e.g...
  10. Novel Mechanisms of Visceral Pain: Functional Properties of Microglia
    Jennifer L Watts; Fiscal Year: 2012
    ..state to a reactive state characterized by increased expression of CDIIb and Ibal proteins and membrane purinergic receptors (P2X4 and P2Y12) and 2) To show that colon-inflammation induced visceral hypersensitivity is reversed by ..
  11. Respiratory Virus Induced Sensory Neuroplasticity
    Bradley J Undem; Fiscal Year: 2013
    ..that viral infections induce, de novo, the expression of the ligand-gated ion channels TRPV1, TRPA1, and purinergic receptors, in the A-fiber neurons rendering them responsive to myriad stimuli they would ordinarily be unresponsive ..
  12. Purinergic Regulation of Bladder Interstitial Cells of Cajal
    Weiqun Yu; Fiscal Year: 2013
    ..Guided by strong preliminary data demonstrating novel purinergic receptors, P2X2/6 and A2a expression on BICC and observing that activation of these receptors induces BSM contraction/..
  13. Mechanisms of Muscle Afferent Sensitization after Ischemia
    Michael P Jankowski; Fiscal Year: 2013
    ..in heat, mechanical and chemo-sensitivity in muscle afferents, which are mediated by upregulation of purinergic receptors during ischemia and acid sensing ion channels after reperfusion leading to muscle pain...
  14. Allergen-induced IL-33 release by the airway epithelium
    SCOTT M O'GRADY; Fiscal Year: 2013
    ..Importantly, suppressing expression of P2 purinergic receptors, either P2Y2R or P2X7R, abolishes the increase in [Ca2+]i and IL-33 release...
  15. Molecular basis of mechanotransduction in bone cells
    Jun You; Fiscal Year: 2013
    ..years, substantial evidence has indicated that extracellular nucleotides, such as ATP, signaling through P2 purinergic receptors, play an important role in the regulation of bone behavior...
  16. Multidisciplinary research training in pulmonary diseases
    Terry L Noah; Fiscal Year: 2013
    ..Our Program provides an opportunity for trainees to develop as scientists and become independent investigators within the national community. ..
  17. Extracellular ATP as regulator of hypoxia-induced vasa vasorum neovascularization
    Evgenia V Gerasimovskaya; Fiscal Year: 2012
    ..The characterization of an angiogenic phenotype of VVEC, including purinergic receptors and coupled signaling pathways, will provide unique targets for therapeutic strategies aimed at inhibiting ..
  18. Purinergic receptors in inflammation
    Wolfgang G Junger; Fiscal Year: 2012
    b>PURINERGIC RECEPTORS IN INFLAMMATION - Chemotaxis allows polymorphonuclear neutrophils (PMN) to rapidly reach infected and inflamed sites. However excessive influx of PMN damages host tissues...
  19. Adrenergic and Purinergic Regulation of Target Cells
    Paul A Insel; Fiscal Year: 2010
    ....
  20. Mechanotransduction of shear stress: from ATP release to CFTR regulation
    BRIAN M BUTTON; Fiscal Year: 2010
    ..ATP release and autocrine/paracrine stimulation of purinergic receptors has been implicated in the regulation of a wide array of cell functions in numerous diverse cell types...
  21. Structure Function Studies on the Mechanisms of P2X3 Receptor Desensitization
    STEVEN ELIAS MANSOOR; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Purinergic receptors (P2X) are proteins that are activated by the binding of ATP and adenosine...
  22. Corneal epithelial adhesion:morphology and biochemistry
    VICKERY E TRINKAUS-RANDALL; Fiscal Year: 2012
    ..We then identified the expression of purinergic receptors (P2Y and P2X) in both epithelium and trigeminal neuronal cells...
  23. Aqueous Humor Outflow Control and Intraocular Pressure
    MORTIMER MORDECAI CIVAN; Fiscal Year: 2013
    ..The current proposal seeks to identify the fundamental step in the regulation of IOP by the purine class of signaling molecules. Successful results may lead to an innovative strategy for lowering IOP in glaucoma. ..
  24. pH-sensing and activation of acid secretion by the V-ATPase
    Sylvie Breton; Fiscal Year: 2013
    ..Some purinergic receptors were proposed as pH sensors, and Aim 1 will test the hypothesis that A-ICs sense urinary pH variations via ..
  25. ATP as the instigator of inflammatory responses to spinal cord injury
    Maiken Nedergaard; Fiscal Year: 2013
    ..abstract_text> ..
  26. Detrusor Overactivity and Bladder Smooth Muscle Dysfunction
    Maryrose P Sullivan; Fiscal Year: 2013
    ..by bladder smooth muscle cells through connexin hemichannels and its autocrine/paracrine activation of purinergic receptors are regulated by caveolae;moreover, loss of caveolae-mediated regulation of this process augments ATP ..
  27. Neuroimaging of Purinergic Pathways in the Human ENS
    FEDIAS LEONTIOU CHRISTOFI; Fiscal Year: 2013
    ....
  28. Pulmonary Surface Liquid Homeostasis
    RICHARD CHARLES BOUCHER; Fiscal Year: 2013
    ..abstract_text> ..
  29. Neutrophil activation and trauma
    Wolfgang G Junger; Fiscal Year: 2013
    ..We identified that ATP release and autocrine signaling via purinergic receptors is the underlying molecular mecha- nism by which HS can suppress but also enhance PMN responses...
  30. Purinergic Regulation of hepatocyte proliferation
    Sundararajah Thevananther; Fiscal Year: 2010
    ..important signaling molecule influencing a variety of liver functions via the activation of cell surface P2 purinergic receptors, and the mitogenic potential of extracellular purines are beginning to be recognized in many other cell ..
  31. Purinergic signaling in taste buds
    Sue Kinnamon; Fiscal Year: 2013
    ..Using mice that lack the purinergic receptors P2X2 and P2X3, we showed that these mice lack gustatory responses to all taste stimuli...
  32. Peripheral Nerve Regeneration and Sensory Neuron Plasticity
    H Richard Koerber; Fiscal Year: 2013
    ..Next we will examine changes in DRG expression of specific trk, GFR[unreadable], ASIC, TRP and purinergic receptors/channels contained in these fibers to indentify possible molecular mechanisms responsible for the changes ..
  33. Injury-induced plasticity of P2X receptor function
    Li Yen M Huang; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Our recent studies of modulation of purinergic receptors (P2XR) in dorsal root ganglion (DRG) neurons show that, after inflammation or nerve injury, the activity of P2X3Rs becomes greatly enhanced The ..
  34. ADENOSINE AND ENDOTHELIN IN MYOCARDIAL REPERFUSION
    John Murray; Fiscal Year: 1993
    ..model systems to determine if ADENOSINE alters ENDOTHELIN gene production via activation of extracellular purinergic receptors. The role of activated neutrophils and platelets will be explored in an in vivo canine model of endothelial ..
  35. MEMBRANE-LIMITED METABOLISM IN MAMMALIAN RED CELLS
    Hyun Kim; Fiscal Year: 1990
    ..The underlying mechanism for this purine mediated glycolytic activation will be ascertained in terms of purinergic receptors, membrane permeability and glycolytic enzyme stimulation...
  36. AFFERENT MECHANISMS UNDERLYING BLADDER PAIN
    WILLIAM DEGROAT; Fiscal Year: 2009
    ..Na+ channels, yanilloid receptors (VRl) which are sensitive to capsaicin, ATP-sensitive purinergic receptors, fast activation R+ channels and acid sensitive cation channels (ASIC), (3) Chronic pathological conditions ..
  37. P2Y-PURINERGIC RECEPTORS
    T Kendall Harden; Fiscal Year: 2012
    ..Completion of the research described here will provide much-needed molecular tools to selectively activate and block uridine nucleotide- and nucleotide sugar-activated P2Y receptors with high affinity and selectivity. ..
  38. ATP in Skin Immunity
    Amanda S Macleod; Fiscal Year: 2013
    ..in skin-resident T cells, evaluate new in vitro and in vivo models of skin inflammation, identify candidate purinergic receptors for drug targeting, and develop new tools for investigating and treating UV-induced skin inflammation and ..
  39. REGULATION OF VASOPRESSIN SECRETION
    Celia D Sladek; Fiscal Year: 2010
    ..Identifying the a1-R signal cascade is critical to understanding why ATP activation of metabotropic purinergic receptors does not induce the same effect as phenylephrine...
  40. Transmission of Taste Signals in Taste Buds
    Thomas E Finger; Fiscal Year: 2010
    ..the hypothesis that in response to stimulation, taste cells release ATP which then activates ionotropic purinergic receptors (P2X2 and P2X3) on the gustatory nerve fibers...
  41. NEURAL CONTROL OF LARGE INTESTINAL MUCOSA
    Helen Cooke; Fiscal Year: 2005
    ..Understanding the regulation of 5-HT may be a key to management of functional bowel disease associated with an excess or reduced number of enterochromaffin cells. ..
  42. ROLE OF ADENYL PURINES IN MYOMETRIAL FUNCTION
    IAIN BUXTON; Fiscal Year: 1993
    ..The working hypothesis is that adenyl purines, both adenosine and ATP from one or more sources stimulate purinergic receptors in myometrial smooth muscle leading to uterine contraction...
  43. P2T PURINERGIC RECEPTORS
    JOSE BOYER; Fiscal Year: 2004
    ..This information should prove useful in the development of new biochemical and pharmacological tools with potential use in the treatment of coagulation-related diseases. ..
  44. Microglia adenine nucleotides and hypoxia
    JYOTI WATTERS; Fiscal Year: 2009
    ..Four specific aims are proposed to test mechanisms by which purinergic receptors influence microglial production of neurotoxic/inflammatory mediators, focusing on purinergic receptor MAP ..
  45. CHEMOTRANSDUCTION IN THE CAROTID BODY
    Robert Fitzgerald; Fiscal Year: 2007
    ..influence of NO, ATP, and ADO on the hypoxia- and hypercapnia-challenged CB; (2) to locate dopaminegic and purinergic receptors in the CB, the carotid sinus nerve, and in cell bodies of the petrosal ganglion (PG) with RT-PCR and ..
  46. Vaccine Development: Purinergic Agonists as Adjuvants
    Richard Granstein; Fiscal Year: 2005
    ..g., anthrax). This application will test the hypothesis that activation of purinergic receptors (i.e...
  47. PURINOCEPTOR CONTROL OF SURFACTANT SECRETION
    Ward Rice; Fiscal Year: 1991
    ....
  48. Cell Volume Regulated Biology in the Liver
    Ravi Chari; Fiscal Year: 2005
    ..We have recently described the presence of 2 mitochondrial P2Y purinergic receptors, mP2Y1 and mP2Y2 that alter mitochondrial Ca2+ uptake in response to changes in cytosolic [ATP]...
  49. CELLULAR BASIS OF AIRWAY SECRETION CLEARANCE COUPLING
    RICHARD BOOKMAN; Fiscal Year: 2004
    ..roles in regulating mucociiary clearance: muscarinic receptors (M3) stimulated by acetylcholine (ACh) and purinergic receptors (P2Y) stimulated by ATP or UTP...
  50. CONTROL OF LUNG SURFACTANT SECRETION IN THE NEWBORN
    SEAMUS ROONEY; Fiscal Year: 1999
    ..Such information is critical so that rational therapeutic interventions for prevention and/or control of the respiratory distress syndrome and other lung diseases may be developed. ..
  51. P2U(P2Y2)-PURINOCEPTOR AND WATER TRANSPORT IN RAT KIDNEY
    BELLAMKONDA KISHORE; Fiscal Year: 2004
    ..Successful completion of these studies will lead to significant insights into the AVP-independent mechanisms of regulation of collecting duct water permeability. ..
  52. Mechanisms of Trauma-induced N-cadherin Expression
    Minh Tran; Fiscal Year: 2005
    ..neurons and astrocytes increase their release of purine nucleosides and nucleotides, which stimulates purinergic receptors that activate signaling pathways and transcription factors...
  53. Nucleotides/fluid flow effects on osteoblastic cells
    Jun You; Fiscal Year: 2004
    ..In specific aims 2 and 3 we will determine the roles of specific P2Y purinergic receptors in fluid flow-induced increases in intracellular calcium concentration...
  54. INORGANIC PYROPHOSPHATE METABOLISM IN ARTHRITIS
    Lawrence Ryan; Fiscal Year: 2006
    ..The intent of these studies is to develop insights into the pathogenesis of CPPD deposition disease so that therapeutic options may be generated. ..
  55. HYPOXIC PULMONARY VASOCANSTRICTION AND RED BLOOD CELLS
    Steven Deem; Fiscal Year: 2002
    ....
  56. IONOTROPIC PURINERGIC RECEPTORS IN RENAL CELLS
    DRAGANA FILIPOVIC; Fiscal Year: 2002
    ..Several classes of purinergic receptors have been identified based on agonist specificity and intracellular signaling pathways...
  57. Mechanotransduction: Puringergic Signaling in Bone
    RANDALL DUNCAN; Fiscal Year: 2009
    ..This release, in turn, results in activation of purinergic receptors (P2) to alter intracellular Ca2+ release...
  58. STRUCTURE AND FUNCTION OF MEMBRANE PROTEINS
    Guido Guidotti; Fiscal Year: 2004
    ..Some central questions are whether ATP release is only used as signal for purinergic receptors which is subsequently terminated by the ectoapyrases, or is AT? extrusion part a way to regulate the amount ..
  59. INTERACTION OF CFTR AND EPITHELIAL NA CHANNELS
    Douglas Eaton; Fiscal Year: 2003
    ..ATP degrading enzymes; determining the rate of release of ATP from A6 monolayers; examining the coupling of purinergic receptors to ENaC inhibition; and determining if the ATP release is via CFTR-dependent exocytosis...
  60. P2T-PURINERGIC RECEPTORS
    JOSE BOYER; Fiscal Year: 1999
    DESCRIPTION (Adapted from investigator's abstract): Platelet P2T- purinergic receptors are unique among P2-purinergic receptors since ADP is the preferred agonist and ATP an antagonist...
  61. Purinergic Mechansism of Nociception
    CHRISTOPHER HONDA; Fiscal Year: 2004
    ..The P2X2 and P2X3 purinergic receptors arechosen for investigation because recent studies suggest that many sensory neurons behave as if they ..
  62. Role of Ion Channels in Cell Death
    William Schilling; Fiscal Year: 2005
    ..Indeed, the effects of MTX are indistinguishable from activation of P2Z/P2X7 purinergic receptors. In this regard, our most recent studies have shown that MTX and P2Z/P2X7 receptor stimulation activate ..