plant lectins

Summary

Summary: Protein or glycoprotein substances of plant origin that bind to sugar moieties in cell walls or membranes. Some carbohydrate-metabolizing proteins (ENZYMES) from PLANTS also bind to carbohydrates, however they are not considered lectins. Many plant lectins change the physiology of the membrane of BLOOD CELLS to cause agglutination, mitosis, or other biochemical changes. They may play a role in plant defense mechanisms.

Top Publications

  1. pmc Alpha-(1-3)- and alpha-(1-6)-D-mannose-specific plant lectins are markedly inhibitory to human immunodeficiency virus and cytomegalovirus infections in vitro
    J Balzarini
    Laboratory of Chemotherapy, Rega Institute for Medical Research, Katholieke Universiteit Leuven, Belgium
    Antimicrob Agents Chemother 35:410-6. 1991
  2. doi Plant lectins as defense proteins against phytophagous insects
    Gianni Vandenborre
    Laboratory of Biochemistry and Glycobiology, Department of Molecular Biotechnology, Ghent University, Coupure Links 653, 9000 Ghent, Belgium
    Phytochemistry 72:1538-50. 2011
  3. pmc Hyperalgesic priming is restricted to isolectin B4-positive nociceptors
    E K Joseph
    Division of Neuroscience, Departments of Medicine and Oral and Maxillofacial Surgery, University of California, San Francisco, CA 94143, USA
    Neuroscience 169:431-5. 2010
  4. pmc Scaleable manufacture of HIV-1 entry inhibitor griffithsin and validation of its safety and efficacy as a topical microbicide component
    Barry R O'Keefe
    Molecular Targets Development Program, National Cancer Institute at Frederick, Frederick, MD 21702, USA
    Proc Natl Acad Sci U S A 106:6099-104. 2009
  5. pmc Diversity in protein glycosylation among insect species
    Gianni Vandenborre
    Laboratory of Agrozoology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium
    PLoS ONE 6:e16682. 2011
  6. doi Plant lectins: targeting programmed cell death pathways as antitumor agents
    Lei Lei Fu
    School of Life Sciences and State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China
    Int J Biochem Cell Biol 43:1442-9. 2011
  7. doi Characterization, molecular cloning, and in silico analysis of a novel mannose-binding lectin from Polygonatum odoratum (Mill.) with anti-HSV-II and apoptosis-inducing activities
    Yun Yang
    School of Life Sciences and State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China
    Phytomedicine 18:748-55. 2011
  8. doi Expression of the TRPM8-immunoreactivity in dorsal root ganglion neurons innervating the rat urinary bladder
    Tokumasa Hayashi
    Department of Urology, Kurume University School of Medicine, Asahimachi 67, Kurume 830 0011, Fukuoka, Japan
    Neurosci Res 65:245-51. 2009
  9. ncbi Antinutritional properties of plant lectins
    Ilka M Vasconcelos
    Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Caixa Postal 6020, Campus do Pici, CEP 60451 970, Fortaleza, CE, Brazil
    Toxicon 44:385-403. 2004
  10. pmc IB4-saporin attenuates acute and eliminates chronic muscle pain in the rat
    Pedro Alvarez
    Department of Oral and Maxillofacial Surgery, University of California San Francisco, CA 94143 0440, USA
    Exp Neurol 233:859-65. 2012

Research Grants

  1. STRUCTURE-FUNCTION RELATIONSHIPS OF LECTINS
    MARILYNN ETZLER; Fiscal Year: 2003
  2. CARBOHYDRATE AND GLYCOPEPTIDE INTERACTIONS WITH LECTINS
    CURTIS BREWER; Fiscal Year: 1990
  3. CARBOHYDRATE AND GLYCOPEPTIDE INTERACTIONS WITH LECTINS
    CURTIS BREWER; Fiscal Year: 1991
  4. PROTEIN-CARBOHYDRATE INTERACTION
    Irwin Goldstein; Fiscal Year: 1991
  5. PROTEIN-CARBOHYDRATE INTERACTION
    Irwin Goldstein; Fiscal Year: 1992
  6. PROTEIN-CARBOHYDRATE INTERACTION
    Irwin Goldstein; Fiscal Year: 1993
  7. NOVEL CELLULAR MECHANISMS IN CORTEX DEVELOPMENT
    Paul Worley; Fiscal Year: 2003
  8. NOVEL CELLULAR MECHANISMS IN CORTEX DEVELOPMENT
    Paul Worley; Fiscal Year: 2000
  9. NOVEL CELLULAR MECHANISMS IN CORTEX DEVELOPMENT
    Paul Worley; Fiscal Year: 2001
  10. NOVEL CELLULAR MECHANISMS IN CORTEX DEVELOPMENT
    Paul Worley; Fiscal Year: 1999

Detail Information

Publications327 found, 100 shown here

  1. pmc Alpha-(1-3)- and alpha-(1-6)-D-mannose-specific plant lectins are markedly inhibitory to human immunodeficiency virus and cytomegalovirus infections in vitro
    J Balzarini
    Laboratory of Chemotherapy, Rega Institute for Medical Research, Katholieke Universiteit Leuven, Belgium
    Antimicrob Agents Chemother 35:410-6. 1991
    ..2 to 0.6 microgram/ml). Unlike DS-5000, however, the plant lectins did not inhibit the replication of other enveloped viruses, except for human cytomegalovirus (50% effective ..
  2. doi Plant lectins as defense proteins against phytophagous insects
    Gianni Vandenborre
    Laboratory of Biochemistry and Glycobiology, Department of Molecular Biotechnology, Ghent University, Coupure Links 653, 9000 Ghent, Belgium
    Phytochemistry 72:1538-50. 2011
    ..lectins that are expressed in response to herbivory by phytophagous insects and the insecticidal properties of plant lectins in general...
  3. pmc Hyperalgesic priming is restricted to isolectin B4-positive nociceptors
    E K Joseph
    Division of Neuroscience, Departments of Medicine and Oral and Maxillofacial Surgery, University of California, San Francisco, CA 94143, USA
    Neuroscience 169:431-5. 2010
    ....
  4. pmc Scaleable manufacture of HIV-1 entry inhibitor griffithsin and validation of its safety and efficacy as a topical microbicide component
    Barry R O'Keefe
    Molecular Targets Development Program, National Cancer Institute at Frederick, Frederick, MD 21702, USA
    Proc Natl Acad Sci U S A 106:6099-104. 2009
    ..Moreover, GRFT-P is potently active in preventing infection of cervical explants by HIV-1 and has no mitogenic activity on cultured human lymphocytes...
  5. pmc Diversity in protein glycosylation among insect species
    Gianni Vandenborre
    Laboratory of Agrozoology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium
    PLoS ONE 6:e16682. 2011
    ..Although the Class of the Insecta is the largest animal taxon on Earth, almost all information concerning glycosylation in insects is derived from studies with only one species, namely the fruit fly Drosophila melanogaster...
  6. doi Plant lectins: targeting programmed cell death pathways as antitumor agents
    Lei Lei Fu
    School of Life Sciences and State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China
    Int J Biochem Cell Biol 43:1442-9. 2011
    ..In this review, we present a brief outline of the representative plant lectins including Ricin-B family, proteins with legume lectin domains and GNA family that can induce cancer cell death ..
  7. doi Characterization, molecular cloning, and in silico analysis of a novel mannose-binding lectin from Polygonatum odoratum (Mill.) with anti-HSV-II and apoptosis-inducing activities
    Yun Yang
    School of Life Sciences and State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, China
    Phytomedicine 18:748-55. 2011
    ..Moreover, POL showed remarkable anti-HSV-II activity towards Vero cells, cytotoxicity towards human melanoma A375 cells and induced apoptosis in a caspase-dependent manner...
  8. doi Expression of the TRPM8-immunoreactivity in dorsal root ganglion neurons innervating the rat urinary bladder
    Tokumasa Hayashi
    Department of Urology, Kurume University School of Medicine, Asahimachi 67, Kurume 830 0011, Fukuoka, Japan
    Neurosci Res 65:245-51. 2009
    ..Our results suggest that the TRPM8-expressing bladder afferents should be classified as Adelta-fibers and C-fibers, while some of these afferents may be involved in nociceptive sensations...
  9. ncbi Antinutritional properties of plant lectins
    Ilka M Vasconcelos
    Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Caixa Postal 6020, Campus do Pici, CEP 60451 970, Fortaleza, CE, Brazil
    Toxicon 44:385-403. 2004
    ..One of the most nutritionally important features of plant lectins is their ability to survive digestion by the gastrointestinal tract of consumers...
  10. pmc IB4-saporin attenuates acute and eliminates chronic muscle pain in the rat
    Pedro Alvarez
    Department of Oral and Maxillofacial Surgery, University of California San Francisco, CA 94143 0440, USA
    Exp Neurol 233:859-65. 2012
    ..Thus, IB4(+) and IB4(-) neurons contribute to acute muscle hyperalgesia induced by diverse insults. However, only IB4+ nociceptors participate in the long term consequence of acute hyperalgesia...
  11. ncbi The latex-fruit syndrome
    S Wagner
    Department of Pathophysiology, University of Vienna, AKH EBO 3Q, Waehringer Guertel 18 20, Austria
    Biochem Soc Trans 30:935-40. 2002
    ..Although there is much information about the plant-derived foods and some data about the allergens involved in the latex-fruit syndrome, it is not always clear whether latex sensitization precedes or follows the onset of food allergy...
  12. pmc Phylogenetic and specificity studies of two-domain GNA-related lectins: generation of multispecificity through domain duplication and divergent evolution
    Els J M Van Damme
    Department of Molecular Biotechnology, Laboratory of Biochemistry and Glycobiology, Ghent University, Coupure Links 653, B 9000 Gent, Belgium
    Biochem J 404:51-61. 2007
    ....
  13. doi Plant lectins: potential antineoplastic drugs from bench to clinic
    Bo Liu
    School of Life Sciences, Sichuan University, Chengdu 610064, China
    Cancer Lett 287:1-12. 2010
    b>Plant lectins, carbohydrate-binding proteins distributed widely in a variety of plant species, have drawn a rising attention for cancer biologists due to their remarkable anti-tumour properties...
  14. ncbi Mannose-binding plant lectins: different structural scaffolds for a common sugar-recognition process
    A Barre
    Institut de Pharmacologie et de Biologie Structurale, UMR-CNRS 5089, 205, route de Narbonne, 31077 Toulouse Cedex 4, France
    Biochimie 83:645-51. 2001
    ..This structural diversity associated with the specific recognition of high-mannose type glycans highlights the importance of mannose-specific lectins as recognition molecules in higher plants...
  15. ncbi Lectins as bioactive plant proteins: a potential in cancer treatment
    Elvira Gonzalez de Mejia
    Department of Food Science and Human Nutrition, University of Illinois at Urbana Champaign, Chicago, IL, USA
    Crit Rev Food Sci Nutr 45:425-45. 2005
    b>Plant lectins, a unique group of proteins and glycoproteins with potent biological activity, occur in foods like wheat, corn, tomato, peanut, kidney bean, banana, pea, lentil, soybean, mushroom, rice, and potato...
  16. ncbi Oxaliplatin acts on IB4-positive nociceptors to induce an oxidative stress-dependent acute painful peripheral neuropathy
    Elizabeth K Joseph
    Department of Medicine, Division of Neuroscience, University of California, San Francisco, San Francisco, California 94143 0440, USA
    J Pain 9:463-72. 2008
    ..We suggest that oxaliplatin acts on IB4 (+)-nociceptors to induce oxidative stress-dependent acute peripheral sensory neuropathy...
  17. doi Utility of Lens culinaris agglutinin-reactive fraction of alpha-fetoprotein and des-gamma-carboxy prothrombin, alone or in combination, as biomarkers for hepatocellular carcinoma
    Richard K Sterling
    Virginia Commonwealth University Health Systems, Richmond, Virginia, USA
    Clin Gastroenterol Hepatol 7:104-13. 2009
    ..This study evaluated their utility, alone or in combination, in a North American population...
  18. ncbi Insecticidal spider venom toxin fused to snowdrop lectin is toxic to the peach-potato aphid, Myzus persicae (Hemiptera: Aphididae) and the rice brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae)
    Rachel E Down
    Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK
    Pest Manag Sci 62:77-85. 2006
    ..Haemolymph taken from the insects that were fed on the fusion protein contained two GNA-immunoreactive proteins of molecular weights corresponding to GNA and to the SFI1/GNA fusion protein...
  19. pmc Plant lectins: the ties that bind in root symbiosis and plant defense
    Peter L De Hoff
    Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA
    Mol Genet Genomics 282:1-15. 2009
    ..Several different classes of plant lectins serve a diverse array of functions...
  20. pmc Griffithsin has antiviral activity against hepatitis C virus
    Philip Meuleman
    Institut Pasteur de Lille, Center for Infection and Immunity of Lille, 1 rue Calmette, 59021 Lille Cedex, France
    Antimicrob Agents Chemother 55:5159-67. 2011
    ..GRFT treatment of chronically infected HCV patients undergoing liver transplantation may be a suitable strategy to prevent infection of the liver allograft...
  21. doi ArtinM, a D-mannose-binding lectin from Artocarpus integrifolia, plays a potent adjuvant and immunostimulatory role in immunization against Neospora caninum
    Mariana R D Cardoso
    Laboratory of Immunoparasitology, Institute of Biomedical Sciences, Federal University of Uberlandia, Av Para 1720, 38400 902 Uberlândia, MG, Brazil
    Vaccine 29:9183-93. 2011
    ....
  22. ncbi Purification and characterization of an N-acetylglucosamine-binding lectin from Koelreuteria paniculata seeds and its effect on the larval development of Callosobruchus maculatus (Coleoptera: Bruchidae) and Anagasta kuehniella (Lepidoptera: Pyralidae)
    Maria Ligia Rodrigues Macedo
    Laboratório de Purificação de Proteínas e suas Funções Biológicas, Departamento de Ciencias Naturais, Universidade Federal de Mato Grosso do Sul UFMS, Avenida Olinto Mancini 1662, Colinos, CP 210, CEP 79603 011 Três Lagoas, MS, Brazil
    J Agric Food Chem 51:2980-6. 2003
    ..65% KpLec produced an LD(50), whereas 0.2% KpLec produced an ED(50). The transformation of genes coding for this lectin could be useful in the development of insect resistance in important agricultural crops...
  23. ncbi Crystal structure of banana lectin reveals a novel second sugar binding site
    Jennifer L Meagher
    Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA
    Glycobiology 15:1033-42. 2005
    ..The residues involved in the second site are common to other lectins in this family, potentially signaling a new group of mannose-specific jacalin-related lectins (mJRL) with two sugar binding sites...
  24. ncbi Fusion proteins containing insect-specific toxins as pest control agents: snowdrop lectin delivers fused insecticidal spider venom toxin to insect haemolymph following oral ingestion
    Elaine Fitches
    Central Science Laboratory, Department for Environment, Food and Rural Affairs, Sand Hutton, York YO41 1LZ, UK
    J Insect Physiol 50:61-71. 2004
    ..SFI1/GNA and similar fusion proteins offer a novel and effective approach for delivering haemolymph active toxins by oral administration, which could be used in crop protection by expression in transgenic plants...
  25. ncbi Identification of some lectin IB4 binding proteins in rat dorsal root ganglia
    Joseph M Fullmer
    Department of Neuroscience, University of Minnesota, 140 Gortner Laboratory, 1479 Gortner Ave, St Paul, MN 55108, USA
    Neuroreport 15:1705-9. 2004
    ..Mass spectrometric analysis of tryptic digests of alpha-galactosidase sensitive bands identified three proteins that bind IB4: the laminin beta 2 chain and the light and medium subunits of neurofilaments...
  26. ncbi Purification and characterization of a lectin from the Indian cultivar of French bean seeds
    A Sharma
    School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
    Protein Pept Lett 17:221-7. 2010
    ..The lectin had no antifungal activity...
  27. doi Immunomodulating effects of Korean mistletoe lectin in vitro and in vivo
    Chan Ho Lee
    College of Pharmacy, Sungkyunkwan University, 300 Cheoncheon dong, Jangan gu, Suwon, Gyeonggi do 440 746, Republic of Korea
    Int Immunopharmacol 9:1555-61. 2009
    ..KML treatment also induced increases in the percentages of CD4(+) and CD8(+) cells in thymus. Our results suggest that KML enhances the immune system through modulation of lymphocytes, NK cells, and macrophages...
  28. doi Overexpression of a wheat jasmonate-regulated lectin increases pathogen resistance
    Qing Hu Ma
    Institute of Botany, Chinese Academy of Sciences, Beijing, China
    Biochimie 92:187-93. 2010
    ..Ta-JA1 and its homologues in maize, rice, sorghum and creeping bentgrass may represent a new type of monocot lectin with a modular structure and diversity of physiological functions in biotic and abiotic stress responses...
  29. pmc Diversity of the superfamily of phloem lectins (phloem protein 2) in angiosperms
    Sylvie Dinant
    Laboratoire de Biologie Cellulaire, Institut National de la Recherche Agronomique, Versailles 78026, France
    Plant Physiol 131:114-28. 2003
    ..The acquisition of additional modular domains in the amino-terminal extensions of other PP2-like proteins could reflect divergence from its phloem function...
  30. ncbi The lectin from leaves of Japanese cycad, Cycas revoluta Thunb. (gymnosperm) is a member of the jacalin-related family
    Fumio Yagi
    Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University, Kagoshima, Japan
    Eur J Biochem 269:4335-41. 2002
    ..Partial amino acid sequences of the lysylendopeptic peptides shows that the lectin might have a repeating structure and belong to the jacalin-related lectin family...
  31. ncbi Use of a library of mutated Maackia amurensis hemagglutinin for profiling the cell lineage and differentiation
    Keisuke Maenuma
    Laboratory of Cancer Biology and Molecular Immunology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan
    Proteomics 8:3274-83. 2008
    ..These results indicated that a library of mutated MAH is useful as a tool for the profiling of various cells based on the variations of the surface glycans...
  32. ncbi Domain-swapped structure of the potent antiviral protein griffithsin and its mode of carbohydrate binding
    Natasza E Ziółkowska
    Protein Structure Section, Macromolecular Crystallography Laboratory, National Cancer Institute, NCI Frederick, Frederick, MD 21702, USA
    Structure 14:1127-35. 2006
    ..Antiviral potency of griffithsin is likely due to the presence of multiple, similar sugar binding sites that provide redundant attachment points for complex carbohydrate molecules present on viral envelopes...
  33. pmc RTM3, which controls long-distance movement of potyviruses, is a member of a new plant gene family encoding a meprin and TRAF homology domain-containing protein
    Patrick Cosson
    UMR Génomique, Diversité et Pouvoir Pathogène, INRA Université Bordeaux 2, Institut de Biologie Végétale Moléculaire, Villenave D Ornon, France
    Plant Physiol 154:222-32. 2010
    ..Taken together, these observations strongly suggest the RTM proteins might form a multiprotein complex in the resistance mechanism to block the long-distance movement of potyviruses...
  34. doi Expression of transient receptor potential ankyrin 1 (TRPA1) in the rat trigeminal sensory afferents and spinal dorsal horn
    Yun Sook Kim
    Department of Oral Anatomy and Neurobiology, Kyungpook National University, Daegu, Korea
    J Comp Neurol 518:687-98. 2010
    ..These results suggest that TRPA1-dependent orofacial and spinal nociceptive input is processed mainly in the superficial laminae of the Vc and DH in a specific manner and may be processed differently between the rostral TSN and Vc...
  35. pmc Binding properties of the N-acetylglucosamine and high-mannose N-glycan PP2-A1 phloem lectin in Arabidopsis
    Julie Beneteau
    UR1268 Unité de Recherches Biopolymères Interactions Assemblages, INRA, F 44300 Nantes, France
    Plant Physiol 153:1345-61. 2010
    ..As many plant lectins have insecticidal activity, we also assessed the effect of PP2-A1 on weight gain and survival in aphids...
  36. doi Healing activity induced by Cramoll 1,4 lectin in healthy and immunocompromised mice
    Cristiane Moutinho Lagos de Melo
    Centro de Pesquisas Aggeu Magalhães CPqAM FIOCRUZ, Pernambuco, Brazil crismout
    Int J Pharm 408:113-9. 2011
    ..Results showed that Cramoll 1,4 lectin was effective in the repair of experimental lesions in mice and can be used as a future cicatricial compound...
  37. ncbi Classification of plant lectins in families of structurally and evolutionary related proteins
    W J Peumans
    Laboratory for Phytopathology and Plant Protection, Katholieke Universiteit Leuven, Willem de Croylaan 42, 3001 Leuven, Belgium
    Adv Exp Med Biol 491:27-54. 2001
    The majority of plant lectins can be classified in seven families of structurally and evolutionary related proteins...
  38. ncbi Lectin analysis of human immunoglobulin G N-glycan sialylation
    M Dalziel
    ICRF Breast Cancer Biology Group, Thomas Guy House, Guy s Hospital, London, UK
    Glycoconj J 16:801-7. 1999
    ..This allowed the construction of quantitative assays capable of measuring both IgG Fab and Fc alpha2,6-sialylation without the need for enzymatic peptide digestion...
  39. ncbi Insecticidal activity of Arum maculatum tuber lectin and its binding to the glycosylated insect gut receptors
    Pralay Majumder
    Plant Molecular and Cellular Genetics, Bose Institute, P1 12 CIT Scheme VII M, Kolkata 700054, India
    J Agric Food Chem 53:6725-9. 2005
    ..erysimi and A. craccivora guts, respectively, using ligand-binding assay. Alpha-d-Mannose was found to be a deterrent to such binding of ATL to the BBMV receptors...
  40. ncbi Construction of hevein (Hev b 6.02) with reduced allergenicity for immunotherapy of latex allergy by comutation of six amino acid residues on the conformational IgE epitopes
    Piia Karisola
    Department of Biological and Environmental Science, University of Jyvaskyla, Jyvaskyla, Finland
    J Immunol 172:2621-8. 2004
    ..Hevein with a highly reduced ability to bind IgE should provide a valuable candidate molecule for immunotherapy of latex allergy and is anticipated to have a low risk of systemic side effects...
  41. pmc Mannose-rich glycosylation patterns on HIV-1 subtype C gp120 and sensitivity to the lectins, Griffithsin, Cyanovirin-N and Scytovirin
    Kabamba B Alexandre
    National Institute for Communicable Diseases, Johannesburg, South Africa
    Virology 402:187-96. 2010
    ..This data support further research on these lectins as potential microbicides in the context of HIV-1 subtype C infection...
  42. ncbi Functional phytohemagglutinin (PHA) and Galanthus nivalis agglutinin (GNA) expressed in Pichia pastoris correct N-terminal processing and secretion of heterologous proteins expressed using the PHA-E signal peptide
    R J Raemaekers
    Department of Biological Sciences, University of Durham, UK
    Eur J Biochem 265:394-403. 1999
    ....
  43. doi A close look at human IgG sialylation and subclass distribution after lectin fractionation
    Johannes Stadlmann
    Department of Chemistry, University of Natural Resources and Applied Life Sciences BOKU, Vienna, Austria
    Proteomics 9:4143-53. 2009
    ..This observation may be relevant when studying the effect of human Fc in murine models of inflammation because mouse IgG subclasses differ substantially in their interaction with receptors...
  44. pmc Enrichment of sialylated IgG by lectin fractionation does not enhance the efficacy of immunoglobulin G in a murine model of immune thrombocytopenia
    Theresa Guhr
    Sanquin Research and Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands
    PLoS ONE 6:e21246. 2011
    ..Our results indicate that SNA lectin fractionation is not a suitable method to enrich IVIg for Fc-sialylated IgG. The use of IVIg enriched for Fab-sialylated IgG abolishes the efficacy of IVIg in the murine PIT model...
  45. ncbi Carbohydrate-binding agents cause deletions of highly conserved glycosylation sites in HIV GP120: a new therapeutic concept to hit the achilles heel of HIV
    Jan Balzarini
    Rega Institute for Medical Research, Katholieke Universiteit Leuven, Belgium
    J Biol Chem 280:41005-14. 2005
    ..It forces HIV to escape drug pressure by deleting the indispensable glycans on its GP120, thereby obligatorily exposing previously hidden immunogenic epitopes on its envelope...
  46. doi Two structurally identical mannose-specific jacalin-related lectins display different effects on human T lymphocyte activation and cell death
    Herve Benoist
    INSERM U 858 I2MR, Toulouse, France
    J Leukoc Biol 86:103-14. 2009
    b>Plant lectins displaying similar single sugar-binding specificity and identical molecular structure might present various biological effects...
  47. ncbi A single amino acid substitution on the surface of a natural hevein isoform (Hev b 6.0202), confers different IgE recognition
    César A Reyes-López
    Instituto de Quimica, Universidad Nacional, Autonoma de Mexico, Circuito Exterior, Cd Universitaria, Mexico, D F 04510, Mexico
    FEBS Lett 580:2483-7. 2006
    ..Overall, these results are relevant to delineate crucial residues involved in this dominant discontinuous epitope...
  48. pmc Related lectins from snowdrop and maize differ in their carbohydrate-binding specificity
    Elke Fouquaert
    Laboratory of Biochemistry and Glycobiology, Department of Molecular Biotechnology, Ghent University, Coupure Links 653, B 9000 Ghent, Belgium
    Biochem Biophys Res Commun 380:260-5. 2009
    ..Our results indicate that gene divergence within the family of GNA-related lectins leads to changes in carbohydrate-binding specificity, as shown on N-glycan arrays...
  49. ncbi Fusion proteins containing neuropeptides as novel insect contol agents: snowdrop lectin delivers fused allatostatin to insect haemolymph following oral ingestion
    Elaine Fitches
    Central Science Laboratory, Department for Environment, Food and Rural Affairs, Sand Hutton, York YO41 1LZ, UK
    Insect Biochem Mol Biol 32:1653-61. 2002
    ....
  50. doi Lectin-based electrophoretic analysis of the expression of the 35 kDa inter-alpha-trypsin inhibitor heavy chain H4 fragment in sera of patients with five different malignancies
    Emida Mohamed
    Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia
    Electrophoresis 29:2645-50. 2008
    ..The lectin-based electrophoretic bioanalytical method adopted in the present study may be used to assess the physiological relevance of ITIH4 fragmentation and its correlation with different malignancies, their stages and progression...
  51. ncbi Structure-function analysis and insights into the reduced toxicity of Abrus precatorius agglutinin I in relation to abrin
    Ashima Bagaria
    Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India
    J Biol Chem 281:34465-74. 2006
    ..This perhaps is not a consequence of any kink formation by a proline residue in the helical segment, as reported by others earlier, but due to fewer interactions that proline can possibly have with the bound substrate...
  52. ncbi Trypanosoma brucei glycoproteins contain novel giant poly-N-acetyllactosamine carbohydrate chains
    Abdelmadjid Atrih
    Division of Biological Chemistry and Molecular Microbiology, The School of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom
    J Biol Chem 280:865-71. 2005
    ..Fluorescence microscopy using fluorescein isothiocyanatericin indicates that ricin ligands are located mainly in the flagellar pocket and in the endosomal/lysosomal system of the trypanosome...
  53. ncbi Lectins: carbohydrate-specific reagents and biological recognition molecules
    Nathan Sharon
    Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel
    J Biol Chem 282:2753-64. 2007
  54. pmc Monomerization of viral entry inhibitor griffithsin elucidates the relationship between multivalent binding to carbohydrates and anti-HIV activity
    Tinoush Moulaei
    Protein Structure Section, Macromolecular Crystallography Laboratory, National Cancer Institute Frederick, Frederick, MD 21702 1201, USA
    Structure 18:1104-15. 2010
    ..Atomic resolution crystal structure of a complex between mGRFT and nonamannoside revealed that a single mGRFT molecule binds to two different nonamannoside molecules through all three carbohydrate-binding sites present on the monomer...
  55. ncbi Binding sites for the plant lectin Bandeiraea simplicifolia I-isolectin B(4) are expressed by nociceptive primary sensory neurones
    M B Gerke
    Neuroscience Laboratory, School of Life Sciences, Queensland University of Technology, Brisbane, Queensland 4001, Australia
    Brain Res 911:101-4. 2001
    ..Consistent with the hypothesis, none of the low threshold primary sensory neurones we sampled expressed lectin binding sites, whilst a subpopulation of the nociceptive neurones did...
  56. ncbi Isolation and characterization of griffithsin, a novel HIV-inactivating protein, from the red alga Griffithsia sp
    Toshiyuki Mori
    Molecular Targets Development Program, Center for Cancer Research, NCI Frederick, Maryland 21702, USA
    J Biol Chem 280:9345-53. 2005
    ..GRFT could be a potential candidate microbicide to prevent the sexual transmission of HIV and AIDS...
  57. doi The determinant of potyvirus ability to overcome the RTM resistance of Arabidopsis thaliana maps to the N-terminal region of the coat protein
    V Decroocq
    UMR GDPP, INRA Université Bordeaux II, IBVM, Centre INRA de Bordeaux, BP 81, 33883 Villenave d Ornon Cedex France
    Mol Plant Microbe Interact 22:1302-11. 2009
    ..Taken together, these findings demonstrate that the potyvirus CP N-terminal region determines the outcome of the interaction with the RTM-mediated resistance...
  58. doi Transgenic cowpea (Vigna unguiculata) seeds expressing a bean alpha-amylase inhibitor 1 confer resistance to storage pests, bruchid beetles
    Siva Kumar Solleti
    Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, Assam 781039, India
    Plant Cell Rep 27:1841-50. 2008
    ..The transgenic protein was active as an inhibitor of porcine alpha-amylase in vitro. Transgenic cowpeas expressing alphaAI-1 strongly inhibited the development of C. maculatus and C. chinensis in insect bioassays...
  59. doi Clinical utility of highly sensitive Lens culinaris agglutinin-reactive alpha-fetoprotein in hepatocellular carcinoma patients with alpha-fetoprotein <20 ng/mL
    Hidenori Toyoda
    Department of Gastroenterology, Ogaki Municipal Hospital, Ogaki, Japan
    Cancer Sci 102:1025-31. 2011
    ..001). In patients with AFP <20 ng/mL, measurements of AFP-L3% by the highly sensitive method before treatment were more useful for diagnosis and prognosis of HCC than by the conventional method...
  60. doi Glycosylation signatures in Drosophila: fishing with lectins
    Gianni Vandenborre
    Laboratory of Agrozoology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, Ghent, Belgium
    J Proteome Res 9:3235-42. 2010
    ..Furthermore, it was demonstrated that a specific glycosylation signature can be associated with a functionally related group of glycoproteins in Drosophila, both in terms of biological process and molecular function...
  61. pmc Broad-spectrum in vitro activity and in vivo efficacy of the antiviral protein griffithsin against emerging viruses of the family Coronaviridae
    Barry R O'Keefe
    Molecular Targets Development Program, Center for Cancer Research, NCI Frederick, Frederick, Maryland 217021, USA
    J Virol 84:2511-21. 2010
    ....
  62. doi Insecticidal activity of scorpion toxin (ButaIT) and snowdrop lectin (GNA) containing fusion proteins towards pest species of different orders
    Elaine C Fitches
    Food and Environment Research Agency, Sand Hutton, York, UK
    Pest Manag Sci 66:74-83. 2010
    ....
  63. doi Lectins: past, present and future
    Nathan Sharon
    Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, Israel
    Biochem Soc Trans 36:1457-60. 2008
    ..This review describes how plant lectins were developed as widely used reagents for the study of glycoconjugates in solution and on cells, and for cell ..
  64. ncbi Expression of frutalin, an alpha-D-galactose-binding jacalin-related lectin, in the yeast Pichia pastoris
    Carla Oliveira
    IBB Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710 057 Braga, Portugal
    Protein Expr Purif 60:188-93. 2008
    ..pastoris and also that linker cleavage might not be essential for lectin sugar specificity...
  65. ncbi High expression of alpha 2, 3-linked sialic acid residues is associated with the metastatic potential of human gastric cancer
    Feng Ling Wang
    Department of Pharmacology, Institute of Materia Medica, Shandong Academy of Medical Sciences, Jinan, Shandong 250062, China
    Cancer Detect Prev 32:437-43. 2009
    ..Herein, we measured the expression of alpha 2, 3-linked sialic acid residues by using a specific lectin as well as the potential of invasion and metastasis of gastric cancer was analyzed...
  66. ncbi Structure of mannose-specific snowdrop (Galanthus nivalis) lectin is representative of a new plant lectin family
    G Hester
    Department of Biochemistry, Virginia Commonwealth University, Richmond 23298, USA
    Nat Struct Biol 2:472-9. 1995
    ..The so formed hybrid beta-sheets are the sites for high affinity mannose binding in the dimer interface. Occupancy observed at corresponding sites in other beta-sheets suggests a potential for twelve sites per tetramer...
  67. pmc Evolutionary history and stress regulation of the lectin superfamily in higher plants
    Shu Ye Jiang
    Temasek Life Sciences Laboratory, 1 Research Link, The National University of Singapore, Singapore 117604
    BMC Evol Biol 10:79. 2010
    ..The availability of full genome sequences from various plant species makes it possible to perform a whole-genome exploration for further understanding their biological functions...
  68. ncbi Structure of the complex of Maclura pomifera agglutinin and the T-antigen disaccharide, Galbeta1,3GalNAc
    X Lee
    Department of Cancer Biology, Cleveland Clinic Research Institute, Cleveland, Ohio 44195, USA
    J Biol Chem 273:6312-8. 1998
    ..The resulting lattice is comparable to those seen for complexes of other lectins with branched glycopeptides...
  69. ncbi Comparative analysis of alpha2,3/2,6-linked N-acetylneuraminic acid and cytokeratin expression in head and neck squamous cell carcinoma
    Martin Chovanec
    First Faculty of Medicine, Institute of Anatomy, Charles University, 128 00 Prague, Czech Republic
    Oncol Rep 12:297-301. 2004
    ..The expression of NeuNAc was assessed by plant lectins: Maackia amurensis agglutinin type 2 (MAA) and Sambucus nigra agglutinin (SNA) specific for alpha2,3NeuNAc and ..
  70. ncbi Changes in sialic acid expression in the lung during intrauterine development of the human fetus
    Andrea Cerná
    Department of Pathology, Medical Faculty Comenius University, Bratislava, Slovakia
    Acta Histochem 104:339-42. 2002
    ..Changes in expression of specific sialic acids during differentiation of the lungs may be useful as marker of the degree of maturity of the foetus...
  71. ncbi Mechanisms of the insecticidal action of TEL (Talisia esculenta lectin) against Callosobruchus maculatus (Coleoptera: Bruchidae)
    Maria Ligia Rodrigues Macedo
    Laboratório de Purificação de Proteínas e suas Funções Biológicas, Departamento de Ciencias Naturais, Universidade Federal de Mato Grosso do Sul, Tres Lagoas, MS, Brazil
    Arch Insect Biochem Physiol 56:84-96. 2004
    b>Plant lectins have insecticidal activity that is probably mediated through their ability to bind carbohydrates...
  72. ncbi Structural basis for the energetics of jacalin-sugar interactions: promiscuity versus specificity
    A Arockia Jeyaprakash
    Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560 012, India
    J Mol Biol 347:181-8. 2005
    ..These results and other available evidence suggest that jacalin is specific to O-glycans and its affinity to N-glycans is extremely weak or non-existent and therefore of limited value in processes involving biological recognition...
  73. ncbi Plant lectins are potent inhibitors of coronaviruses by interfering with two targets in the viral replication cycle
    Els Keyaerts
    Laboratory of Clinical and Epidemiological Virology, Department of Microbiology and Immunology, Rega Institute for Medical Research, University of Leuven, Minderbroedersstraat 10, 3000 Leuven, Belgium
    Antiviral Res 75:179-87. 2007
    We describe the antiviral activity of plant lectins with specificity for different glycan structures against the severe acute respiratory syndrome coronavirus (SARS-CoV) and the feline infectious peritonitis virus (FIPV) in vitro...
  74. ncbi The glycosylation of thymic microenvironments. A microscopic study using plant lectins
    Lutz C Paessens
    Department of Molecular Cell Biology and Immunology, VU University Medical Center, 1081 BT Amsterdam, The Netherlands
    Immunol Lett 110:65-73. 2007
    ..of the human thymus we studied the glycosylation of the human thymic microarchitecture by using plant lectins in situ...
  75. ncbi Structural basis of the carbohydrate specificities of jacalin: an X-ray and modeling study
    A Arockia Jeyaprakash
    Molecular Biophysics Unit, UGC Centre of Advanced Study, Indian Institute of Science, Bangalore 560 012, India
    J Mol Biol 332:217-28. 2003
    ..They also lead to fresh insights into the nature of the binding of glycoproteins by jacalin...
  76. pmc Interaction of the tobacco lectin with histone proteins
    Dieter Schouppe
    Laboratory of Biochemistry and Glycobiology, Department of Molecular Biotechnology, Faculty of Bioscience Engineering, Ghent University, B 9000 Ghent, Belgium
    Plant Physiol 155:1091-102. 2011
    The tobacco (Nicotiana tabacum) agglutinin or Nictaba is a member of a novel class of plant lectins residing in the nucleus and the cytoplasm of tobacco cells...
  77. pmc Functional characterization of HFR1, a high-mannose N-glycan-specific wheat lectin induced by Hessian fly larvae
    Subhashree Subramanyam
    Department of Agronomy, Purdue University, West Lafayette, Indiana 47907, USA
    Plant Physiol 147:1412-26. 2008
    ..Thus, HFR1 is an antinutrient to dipteran larvae and may play a significant role in deterring Hessian fly larvae from feeding on resistant wheat plants...
  78. ncbi Crystal structures of artocarpin, a Moraceae lectin with mannose specificity, and its complex with methyl-alpha-D-mannose: implications to the generation of carbohydrate specificity
    J V Pratap
    Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India
    J Mol Biol 317:237-47. 2002
    ....
  79. ncbi In vitro inhibition of Streptococci binding to enamel acquired pellicle by plant lectins
    E H Teixeira
    Medical School of Sobral, Federal University of Ceara, Brazil
    J Appl Microbiol 101:111-6. 2006
    ..Therefore, this work aimed to evaluate the potential of six lectins, extracted from seeds of Leguminosae family members, to inhibit the adherence of five streptococci species to acquired pellicle in vitro...
  80. ncbi Tissue and serum alpha 2-3- and alpha 2-6-linkage specific sialylation changes in oral carcinogenesis
    Manisha H Shah
    Biochemistry Research Division, The Gujarat Cancer and Research Institute, NCH Campus, Asarwa, Ahmedabad 380 016, India
    Glycoconj J 25:279-90. 2008
    ..Thus, the data suggest potential utility of sialylation markers in early detection, prognostication and treatment monitoring of oral cancer...
  81. doi Crystal structures of human secretory proteins ZG16p and ZG16b reveal a Jacalin-related β-prism fold
    Mayumi Kanagawa
    Structural Glycobiology Team, Systems Glycobiology Research Group, Chemical Biology Department, RIKEN Advanced Science Institute, 2 1 Hirosawa, Wako, Saitama 351 0198, Japan
    Biochem Biophys Res Commun 404:201-5. 2011
    ..ZG16p is occupied by a glycerol molecule, mimicking the mannose bound to Jacalin-related mannose-binding-type plant lectins such as Banlec...
  82. ncbi The crystal structure of the Calystegia sepium agglutinin reveals a novel quaternary arrangement of lectin subunits with a beta-prism fold
    Yves Bourne
    AFMB CNRS, CNRS UMR6098, F13402 Marseille Cedex 20, France
    J Biol Chem 279:527-33. 2004
    ..diversity in the mode of association of lectin subunits is frequently observed, the oligomeric assemblies of plant lectins display small variations within a single family...
  83. doi Isolation and characterization of a novel lectin with antifungal and antiproliferative activities from Sophora alopecuroides seeds
    Tingting Li
    Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, China
    Acta Biochim Biophys Sin (Shanghai) 44:606-13. 2012
    ..SAL suppresses the proliferation of human cervical cancer cells (HeLa) at an IC(50) of 6.25 μM (P< 0.05). But it has no inhibiting effect on bacteria. This is the first report of a lectin from seeds of S. alopecuroides...
  84. ncbi Mistletoe viscotoxins increase natural killer cell-mediated cytotoxicity
    Julie Tabiasco
    Institut National de la Santé et de la Recherche Médicale U563 and Service de spectrométrie de masse de l IFR 30, CHU Purpan, BP3028, Toulouse, France
    Eur J Biochem 269:2591-600. 2002
    ..Microphysiometry and assays of cell killing indicate that, within such nontoxic concentrations, viscotoxins do not activate NK cells, but act on cell conjugates to increase the resulting lysis...
  85. ncbi Pro-inflammatory effect of Arum maculatum lectin and role of resident cells
    Veruska B M Alencar
    Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceara, Brazil
    Int J Biochem Cell Biol 37:1805-14. 2005
    ..maculatum agglutinin presents pro-inflammatory activity, inducing neutrophil migration by two ways, one which is independent on resident cells and another one dependent on the presence of these cells...
  86. doi Dietary garlic (Allium sativum) lectins, ASA I and ASA II, are highly stable and immunogenic
    Fatima Clement
    Department of Biochemistry and Nutrition, Central Food Technological Research Institute CSIR Laboratory, Mysore, Karnataka State, India
    Int Immunopharmacol 10:1161-9. 2010
    ....
  87. ncbi Recognition profile of Morus nigra agglutinin (Morniga G) expressed by monomeric ligands, simple clusters and mammalian polyvalent glycotopes
    Tanuja Singh
    Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, Chang Gung University, Kwei San, Tao Yuan 333, Taiwan
    Mol Immunol 44:451-62. 2007
    ..These results provide evidence for the binding of this lectin to dense cell surface T/Tn glycoconjugates and facilitate future usage of this lectin in biotechnological and medical applications...
  88. ncbi Purification and characterization of a lectin from seeds of Vatairea macrocarpa Duke
    B S Cavada
    Depto de Bioquímica e Biologia Molecular, Universidade Federal do Ceara, Fortaleza CE, Brazil
    Phytochemistry 49:675-80. 1998
    ..Size-exclusion chromatography indicated that, at neutral pH, VML is predominantly a dimeric (70 kDa) protein, although tetramers (115 kDa) and larger aggregates (300 kDa) were also present...
  89. ncbi The Gal/GalNAc-specific lectin from the plant pathogenic basidiomycete Rhizoctonia solani is a member of the ricin-B family
    L Candy
    Institut de Pharmacologie et Biologie Structurale, UMR-CNRS 5089, 205 route de Narbonne, Toulouse Cedex 4, 31077, France
    Biochem Biophys Res Commun 282:655-61. 2001
    ..Our results demonstrate an obvious evolutionary relationship between some fungal and plant lectins, but also provide evidence for the occurrence of a lectin consisting of subunits corresponding to a single ..
  90. doi Characterization of IgE-binding epitopes of peanut (Arachis hypogaea) PNA lectin allergen cross-reacting with other structurally related legume lectins
    Pierre Rouge
    Universite de Toulouse, UMR UPS CNRS 5546, 24 Chemin de Borde Rouge, 31326 Castanet Tolosan, France
    Mol Immunol 47:2359-66. 2010
    ..Peanut lectin PNA and other legume lectins have been characterized as potential allergens for patients allergic to edible legume seeds. However, the clinical significance of the lectin-IgE interaction has to be addressed...
  91. ncbi Insect resistance to Nilaparvata lugens and Cnaphalocrocis medinalis in transgenic indica rice and the inheritance of gna+sbti transgenes
    Guiying Li
    State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China
    Pest Manag Sci 61:390-6. 2005
    ..In all, we obtained nine lines of R3 transgenic plants, including one pure line, which had better resistance to both N lugens and C medinalis than wild-type plants...
  92. ncbi Isolation and characterization of major banana allergens: identification as fruit class I chitinases
    R Sanchez-Monge
    Unidad de Bioquimica, Departamento de Biotecnologia, E T S Ingenieros Agronomos, Madrid, Spain
    Clin Exp Allergy 29:673-80. 1999
    ..Objective To purify and characterize the 30-37 kDa banana allergens...
  93. doi Analysis of B-cell epitopes from the allergen Hev b 6.02 revealed by using blocking antibodies
    Martha Pedraza-Escalona
    Instituto de Quimica, Universidad Nacional Autonoma de Mexico, Circuito Exterior, Cd Universitaria, Coyoacan, Mexico, D F 04510, Mexico
    Mol Immunol 46:668-76. 2009
    ..02-IgE interactions. Antibodies recognizing this important allergen have potential to be used to diagnose and ultimately treat latex allergy...
  94. ncbi Latex allergy within a cohort of not-at-risk subjects with respiratory symptoms: prevalence of latex sensitization and assessment of diagnostic tools
    Adriano Mari
    Center of Clinical and Experimental Allergology, IDI IRCCS, Rome, Italy
    Int Arch Allergy Immunol 143:135-43. 2007
    ..Many cases of NRL allergy remain undiagnosed due to misreporting of symptoms by the patients or lack of proper questions asked by the physician...
  95. ncbi Isolated hevein-like domains, but not 31-kd endochitinases, are responsible for IgE-mediated in vitro and in vivo reactions in latex-fruit syndrome
    Piia Karisola
    Department of Biological and Environmental Science, University of Jyvaskyla, Jyvaskyla, Finland
    J Allergy Clin Immunol 115:598-605. 2005
    ..IgE of these patients has been shown to bind endochitinases containing an N-terminal hevein-like domain (HLD). However, evidence on 31-kd endochitinase-induced reactions in vivo is lacking...
  96. ncbi Structural mechanism governing the quaternary organization of monocot mannose-binding lectin revealed by the novel monomeric structure of an orchid lectin
    Wei Liu
    Center for Structural and Molecular Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, People s Republic of China
    J Biol Chem 280:14865-76. 2005
    ..Potential carbohydrate recognition sites and biological implications of the orchid lectin based on its monomeric state are also discussed in this paper...
  97. pmc Genetic mapping of microsatellite markers around the arcelin bruchid resistance locus in common bean
    Matthew W Blair
    International Center for Tropical Agriculture, Cali, Colombia
    Theor Appl Genet 121:393-402. 2010
    ....
  98. ncbi Hevein-like protein domains as a possible cause for allergen cross-reactivity between latex and banana
    J H Mikkola
    National Public Health Institute, Institute of Occupational Health, Helsinki, Finland
    J Allergy Clin Immunol 102:1005-12. 1998
    ..Patients allergic to natural rubber latex (NRL) frequently exhibit immediate hypersensitivity reactions to banana, but the molecular basis of these putative cross-reactions is unknown...
  99. ncbi A new lectin in red kidney beans called PvFRIL stimulates proliferation of NIH 3T3 cells expressing the Flt3 receptor
    J G Moore
    ImClone Systems Incorprated, New York, New York 10014, USA
    Biochim Biophys Acta 1475:216-24. 2000
    ..These observations led to the hypothesis that PvFRIL acts in a dominant manner to preserve progenitor viability and prevent proliferation and differentiation...
  100. doi Targeted drug delivery: binding and uptake of plant lectins using human 5637 bladder cancer cells
    Verena E Plattner
    Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Vienna, Austria
    Eur J Pharm Biopharm 70:572-6. 2008
    ..effort to detect novel strategies in bladder cancer therapy, the potential and the applicability of different plant lectins was investigated using 5637 cells as a model for human urinary carcinoma...
  101. ncbi Ferritin acts as the most abundant binding protein for snowdrop lectin in the midgut of rice brown planthoppers (Nilaparvata lugens)
    J Du
    Department of Biological Sciences, University of Durham, South Road, Durham, UK
    Insect Biochem Mol Biol 30:297-305. 2000
    ..The fersub1 cDNA carries a stem-loop structure (iron-responsive element) upstream from the start codon, similar to structures that have been shown to play a role in the control of ferritin synthesis in other insects...

Research Grants78

  1. STRUCTURE-FUNCTION RELATIONSHIPS OF LECTINS
    MARILYNN ETZLER; Fiscal Year: 2003
    DESCRIPTION: (Adapted from applicants abstract) Plant lectins are major tools in studies of the protein-carbohydrate interactions that play a key role in establishing the specificity of a wide variety of biological recognition and ..
  2. CARBOHYDRATE AND GLYCOPEPTIDE INTERACTIONS WITH LECTINS
    CURTIS BREWER; Fiscal Year: 1990
    ..b>Plant lectins have a wide number of biological applications, including their use in exploring the structure and dynamic ..
  3. CARBOHYDRATE AND GLYCOPEPTIDE INTERACTIONS WITH LECTINS
    CURTIS BREWER; Fiscal Year: 1991
    ..b>Plant lectins have a wide number of biological applications, including their use in exploring the structure and dynamic ..
  4. PROTEIN-CARBOHYDRATE INTERACTION
    Irwin Goldstein; Fiscal Year: 1991
    This proposal represents a continuation of our studies on the isolation and characterization of plant lectins, and of their application to biomedical research...
  5. PROTEIN-CARBOHYDRATE INTERACTION
    Irwin Goldstein; Fiscal Year: 1992
    This proposal represents a continuation of our studies on the isolation and characterization of plant lectins, and of their application to biomedical research...
  6. PROTEIN-CARBOHYDRATE INTERACTION
    Irwin Goldstein; Fiscal Year: 1993
    This proposal represents a continuation of our studies on the isolation and characterization of plant lectins, and of their application to biomedical research...
  7. NOVEL CELLULAR MECHANISMS IN CORTEX DEVELOPMENT
    Paul Worley; Fiscal Year: 2003
    ..Binding sites for plant lectins (concanavilin A or pea lectin) are enriched in the developing cortex, but few natural ligands are known...
  8. NOVEL CELLULAR MECHANISMS IN CORTEX DEVELOPMENT
    Paul Worley; Fiscal Year: 2000
    ..Binding sites for plant lectins (concanavilin A or pea lectin) are enriched in the developing cortex, but few natural ligands are known...
  9. NOVEL CELLULAR MECHANISMS IN CORTEX DEVELOPMENT
    Paul Worley; Fiscal Year: 2001
    ..Binding sites for plant lectins (concanavilin A or pea lectin) are enriched in the developing cortex, but few natural ligands are known...
  10. NOVEL CELLULAR MECHANISMS IN CORTEX DEVELOPMENT
    Paul Worley; Fiscal Year: 1999
    ..Binding sites for plant lectins (concanavilin A or pea lectin) are enriched in the developing cortex, but few natural ligands are known...
  11. NOVEL CELLULAR MECHANISMS IN CORTEX DEVELOPMENT
    Paul Worley; Fiscal Year: 2002
    ..Binding sites for plant lectins (concanavilin A or pea lectin) are enriched in the developing cortex, but few natural ligands are known...
  12. CELL FUSION IN CHLAMYDOMONAS REINHARDI
    Richard Weiss; Fiscal Year: 1980
    ..Selected plant lectins will be studied to identify which of the fusion polypeptides present on the mating structure are involved in ..
  13. BIOCHEMISTRY OF MAMMARY TUMOR ANTIGENS
    Milan Tomana; Fiscal Year: 1980
    ..The antigens of MMTV isolated by ion exchange chromatography or affinity chromatography with plant lectins, gel filtration and/or preparative polyacrylamide gel electrophoresis in sodium dodecyl sulfate were used to ..
  14. CARBON-13 STUDIES OF CON A-CELL RECEPTOR INTERACTIONS
    A Sherry; Fiscal Year: 1980
    b>Plant lectins are widely used to study changes in cell membrane fluidity induced by chemical carcinogens and viruses, disrupt tumorous cell membrane functions in vivo, promote intracellular biological responses and cell division, and ..
  15. CARBOHYDRATE FRACTIONATION FACILITY
    Pamela Stanley; Fiscal Year: 1990
    ..for use in lectin-affinity HPLC which separates carbohydrates according to their abilities to bind different plant lectins. The third system in the facility will be the Dionex Carbohydrate Gradient HPLC system, a novel method ..
  16. A NEW APPROACH TO THE STUDY OF CAPACITATION
    Prudence Talbot; Fiscal Year: 1980
    ..A new approach using plant lectins to study the sperm surface is proposed...
  17. High-specificity affinity reagents for N-glycosylation site mapping and glycomics
    Robert Woods; Fiscal Year: 2009
    ..Further, in contrast to plant lectins, engineered lectenz are derived from enzymes that have exquisite substrate specificities and low toxicities...
  18. OXALINS AND CELL PROLIFERATION AND REGULATION
    Gordon Hamilton; Fiscal Year: 1993
    ..with the known metabolic effects of such stimulants (among the hormones and stimulants to be investigated are: plant lectins, phorbol esters, interleukins, calcium ionophores, prostaglandins, leukotrienes, etc...
  19. Investigating Complex Glycans on Biological Surfaces
    Ram Sasisekharan; Fiscal Year: 2010
    ..b>Plant lectins have traditionally been used to investigate distribution of glycan structural motifs (recognized by the ..
  20. AN ANALYSIS OF MAMMALIAN OOGENESIS AND FOLLICULOGENESIS
    EVERETT ANDERSON; Fiscal Year: 1980
    ..characteristics of the plasma membrane of the granulosa cell that accompany its differentiation by use of plant lectins, and the selective permeability of the basement membrane as it relates to the maintenance of the unique ..
  21. EFFECT OF MICROTUBULAR PROTEINS ON CELL SURFACES
    Richard Berlin; Fiscal Year: 1980
    ..For example, transformed cells are more agglutinable by various plant lectins than their normal counterparts...
  22. STRUCTURE-FUNCTION RELATIONSHIPS OF LECTINS
    MARILYNN ETZLER; Fiscal Year: 2001
    DESCRIPTION: (Adapted from applicants abstract) Plant lectins are major tools in studies of the protein-carbohydrate interactions that play a key role in establishing the specificity of a wide variety of biological recognition and ..
  23. STRUCTURE-FUNCTION RELATIONSHIPS OF LECTINS
    MARILYNN ETZLER; Fiscal Year: 2000
    DESCRIPTION: (Adapted from applicants abstract) Plant lectins are major tools in studies of the protein-carbohydrate interactions that play a key role in establishing the specificity of a wide variety of biological recognition and ..
  24. ENDOTHELIAL PERMEABILITY IN AIRWAY ANGIOGENESIS
    Donald McDonald; Fiscal Year: 2000
    ..The cells also have an abnormal binding pattern to certain plant lectins and are abnormally sensitive to inflammatory mediators that cause plasma leakage...
  25. ENDOTHELIAL PERMEABILITY IN AIRWAY ANGIOGENESIS
    Donald McDonald; Fiscal Year: 1999
    ..The cells also have an abnormal binding pattern to certain plant lectins and are abnormally sensitive to inflammatory mediators that cause plasma leakage...
  26. STRUCTURE-FUNCTION RELATIONSHIPS OF LECTINS
    MARILYNN ETZLER; Fiscal Year: 2002
    DESCRIPTION: (Adapted from applicants abstract) Plant lectins are major tools in studies of the protein-carbohydrate interactions that play a key role in establishing the specificity of a wide variety of biological recognition and ..
  27. A Real-Time, Label-Free Diagnostic Platform with Glycan Arrays
    Charles Greef; Fiscal Year: 2007
    ..During Phase I of this project, AlphaSniffer will develop glycan microarrays for the detection of two plant lectins, Concanavalin A and jacalin as model analytes to investigate the feasibility of incorporating glycomic tools ..
  28. SURFACE BIOCHEMISTRY OF EMBRYONIC CEREBELLAR CELLS
    MARY HATTEN; Fiscal Year: 1980
    ..from embryonic mouse cerebellum at different developmental stages and characterized by affinity binding to plant lectins immobilized on Sepharose...
  29. MECHANISMS UNDERLYING EXPERIMENTAL TERATOGENESIS
    Devendra Kochhar; Fiscal Year: 1980
    ..In investigations on changes in cell surfaces during normal and abnormal development, we will use plant lectins (radio-labeled or fluorescein-conjugated) in suspensions of cells derived from limb mesenchyme...
  30. FACTORS IN HUMAN LYMPHOCYTE COLONY FORMATION
    HENRY HERROD; Fiscal Year: 1980
    ..In addition to plant lectins other potential inducers of lymphoid colony formation will be examined...
  31. Novel Molecular Tools for Transsynaptic Circuit Analysis
    Haig Keshishian; Fiscal Year: 2006
    ..The constructs are based on both plant lectins (WGA and BL) and the tetanus toxin C chain (TTC), molecules that cross synapses...
  32. Novel Molecular Tools for Transsynaptic Circuit Analysis
    Haig Keshishian; Fiscal Year: 2007
    ..The constructs are based on both plant lectins (WGA and BL) and the tetanus toxin C chain (TTC), molecules that cross synapses...
  33. Colonic Cells:Non-Invasive Isolation Technology
    Padmanabhan Nair; Fiscal Year: 2003
    ..carbohydrate motifs linked to tumorigenesis will be examined by flow cytometry using fluorescently labeled plant lectins that are now to be specific ligands...
  34. Shotgun Glycomics: Linking Glycan Structure and Function
    David Smith; Fiscal Year: 2009
    ..The exquisite specificity of plant lectins, for example, has been known and documented for decades...
  35. IMMUNE ALTERATIONS MEDIATED BY CONDITIONING
    DONALD LYSLE; Fiscal Year: 1990
    ..The immunologic assessments will include the : (1) mitogenic response to plant lectins, (2) activity of natural killer cells, (3) production of interleukin-1 and interleukin-2, (4) quantification ..
  36. TRANSMITTER INTERACTIONS OF SEPTO-HIPPOCAMPAL NEURONS
    TERESA MILNER; Fiscal Year: 1990
    ..immunocytochemical labels combined with the histochemical demonstration of transport horseradish peroxidase or plant lectins for the identification of neuronal pathways...
  37. LOCATION AND FUNCTION OF CARBOHYDRATES IN THE BRAIN
    John Wood; Fiscal Year: 1980
    ..sugars in oligosaccharide chains on carbohydrates lining smooth membrane systems within neurons using plant lectins with different sugar specificities in combination with peroxidase labeling techniques to visualize the sites ..
  38. INHERITED RETINAL DEGENERATION
    Barbara McLaughlin; Fiscal Year: 1980
    ..Cell surface carbohydrates will be visualized by the use of plant lectins which bind to certain sugar residues, in combination with perioxidase labeling techniques to visualize the ..
  39. Colonic Cells:Non-Invasive Isolation Technology
    Padmanabhan Nair; Fiscal Year: 2002
    ..carbohydrate motifs linked to tumorigenesis will be examined by flow cytometry using fluorescently labeled plant lectins that are now to be specific ligands...
  40. AFFINITY PARTITIONING OF SUBSYNAPTIC MEMBRANES
    Steven Flanagan; Fiscal Year: 1980
    ..Purification will be based upon the interaction of plant lectins with saccharide-rich membrane fragments...
  41. RETINAL DIFFERENTIATION, CELL INTERACTIONS & PLASTICITY
    Janet Blanks; Fiscal Year: 1980
    ..will be studied by using microbial carbohydrate-specific antibodies plus peroxidase or ferritin conjugated plant lectins. Glycoproteins will be localized during synapse formation by EM autoradiography after intravitreal injections ..
  42. GLYCOSYLATION DEFECTS OF LECTIN-RESISTANT TUMOR CELLS
    Pamela Stanley; Fiscal Year: 1991
    ..The mutants were selected for resistance to plant lectins and exhibit properties typical of those that we have previously shown to arise from altered carbohydrate ..
  43. GLYCOSYLATION DEFECTS OF LECTIN-RESISTANT TUMOR CELLS
    Pamela Stanley; Fiscal Year: 1990
    ..The mutants were selected for resistance to plant lectins and exhibit properties typical of those that we have previously shown to arise from altered carbohydrate ..
  44. MOLECULAR STUDY OF RIBOSOMAL TOXINS AND APPLICATIONS
    John Perentesis; Fiscal Year: 1990
    Recently, potent toxic plant lectins have been conjugated to monoclonal antibodies directed against specific cell surface determinants to provide new avenues for selective cancer therapy...
  45. CNS DEVELOPMENT: A GENETIC AND CELL SURFACE STUDY
    Karl Herrup; Fiscal Year: 1980
    ..Finally, histochemical staining of developing mutant and wild-type cerebellum with labeled plant lectins reveals the carbohydrate "signature" of the developing cerebellum and how this pattern changes in the mutants.
  46. NMR INVESTIGATIONS OF MEMBRANE INTERACTIONS
    James Prestegard; Fiscal Year: 1990
    ..b>Plant lectins, such as ricin, binding to artificial lipid bilayer systems, will be used to mimic recognition and aggregation ..
  47. LECTIN-DEPENDENT CELL-MEDIATED CYTOTOXICITY
    DAVID LAUX; Fiscal Year: 1980
    ..nonspecific cellular cytotoxic reactions as mediated by immune lymphoid populations through interaction with plant lectins (lectin-dependent cell-mediated cytotoxicity)...
  48. PROTEIN-CARBOHYDRATE INTERACTION
    Irwin Goldstein; Fiscal Year: 1980
    ..b>Plant lectins are being isolated, purified and characterized and are being used as probes to study glycoconjugates (..
  49. SYNTHETIC GLYCOSIDES FOR CANCER RESEARCH
    Khushi Matta; Fiscal Year: 1980
    ..The synthetic carbohydrate derivatives will be used for the study of glycosidases, glycosyltransferases and plant lectins. Much emphasis is being made on the preparation of highly specific acceptors for glycosyl-transferases.
  50. RHEOLOGICAL DETERMINANTS OF MICROVASCULAR FUNCTION
    HERBERT LIPOWSKY; Fiscal Year: 2001
    ....
  51. Glial Cell Function on SVZ Neurogenesis
    Angelique Bordey; Fiscal Year: 2007
    ....
  52. Choriocapillaris Activation in Macular Degeneration
    Robert Mullins; Fiscal Year: 2009
    ..Interdisciplinary experiments involving genetics, computer engineering, biochemistry, cell culture and microscopy are expected to provide new information about the potential role of choriocapillaris activation in the development of AMD. ..
  53. Exploring Tai Chi as a Behavioral Intervention for Heart Failure Patients
    Laura S Redwine; Fiscal Year: 2010
    ..Historically, HF patients were counseled to limit physical activities. However, recent studies show that moderate exercise can be performed safely in properly evaluated NYHA II-III HF patients. ..
  54. Potential target molecules for ischemic retinopathies
    STANLEY VINORES; Fiscal Year: 2009
    ..If multiple target molecules are identified, combination therapies can be devised to provide maximum therapeutic effectiveness while minimizing adverse effects. ..
  55. GABA Signaling in the Postnatal Subventricular Zone
    Angelique Bordey; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  56. Afferent modulation in bladde dysfunction
    Naoki Yoshimura; Fiscal Year: 2006
    ..abstract not provided ..
  57. LOCALIZATION OF BLOOD-RETINAL BARRIER BREAKDOWN SITES
    STANLEY VINORES; Fiscal Year: 1993
    ..These experiments could provide new insights concerning the BRB and how it might be modulated, which could serve as a basis of the design of new treatment approaches for macular edema...
  58. PSYCHOLOGICAL INTERVENTION FOR WOMEN WITH BREAST CANCER
    BARBARA ANDERSEN; Fiscal Year: 2003
    ....
  59. AFFERENT PLASTICITY UNDERLYING URETHRAL AND PELVIC PAIN
    Naoki Yoshimura; Fiscal Year: 2009
    ..This is recognized as a high priority in the urologic care of patients with IC/CPPS. ..
  60. Cerebellar Neurogensis
    Angelique Bordey; Fiscal Year: 2010
    ....
  61. Muller glia and neuronal regeneration in the retina
    Andy Fischer; Fiscal Year: 2009
    ..This information could be applied to not only stimulate neural regeneration to treat sight-threatening diseases of the retina, but also to control gliosis that may be detrimental to the pathogenesis of retinal disorders. ..
  62. Muller glia and neuronal regeneration in the retina
    Andy Fischer; Fiscal Year: 2008
    ..This information could be applied to not only stimulate neural regeneration to treat sight-threatening diseases of the retina, but also to control gliosis that may be detrimental to the pathogenesis of retinal disorders. ..
  63. Role of Glycosylation of Dsg1 in Pemphigus acantholysis
    Ning Li; Fiscal Year: 2007
    ..Additionally, the data generated and broad knowledge gained will support the candidate to develop an independent career. ..
  64. Role of Apoptosis in Pemphigus
    Ning Li; Fiscal Year: 2007
    ..The work is likely to lead to a more comprehensive R01 project investigating the apoptotic pathways involved in pemphigus and its pathogenic role in pemphigus. [unreadable] [unreadable] [unreadable] [unreadable]..
  65. Muller glia and neuronal regeneration in the retina
    Andy Fischer; Fiscal Year: 2006
    ..This information could be applied to not only stimulate neural regeneration to treat sight-threatening diseases of the retina, but also to control gliosis that may be detrimental to the pathogenesis of retinal disorders. ..
  66. Muller glia and neuronal regeneration in the retina
    Andy Fischer; Fiscal Year: 2007
    ..This information could be applied to not only stimulate neural regeneration to treat sight-threatening diseases of the retina, but also to control gliosis that may be detrimental to the pathogenesis of retinal disorders. ..
  67. Fucosylated cell surface molecules in tumor invasion
    STEVEN DOMINO; Fiscal Year: 2005
    ..and in 1,2-dimethylhydrazine-induced tumors, in the presence and absence of cell surface alpha(1,2)fucose by crossing mice into the Fut1 and Fut2 null background. ..
  68. Molecular Mechanisms of Methamphetamine Neurotoxicity
    David Thomas; Fiscal Year: 2008
    ..Therefore, it is imperative to understand the mechanisms of how METH functions, and how it damages the CNS. ..