lectins

Summary

Summary: Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.

Top Publications

  1. ncbi Glycosylation in cellular mechanisms of health and disease
    Kazuaki Ohtsubo
    Howard Hughes Medical Institute and Department of Cellular and Molecular Medicine, 9500 Gilman Drive MC0625, University of California, San Diego, La Jolla, CA 92093, USA
    Cell 126:855-67. 2006
  2. ncbi Siglecs and their roles in the immune system
    Paul R Crocker
    Wellcome Trust Biocentre, College of Life Sciences, University of Dundee, Dundee DD 15EH, UK
    Nat Rev Immunol 7:255-66. 2007
  3. pmc Role of chitin and chitinase/chitinase-like proteins in inflammation, tissue remodeling, and injury
    Chun Geun Lee
    Section of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520 8057, USA
    Annu Rev Physiol 73:479-501. 2011
  4. pmc C1q and mannose binding lectin engagement of cell surface calreticulin and CD91 initiates macropinocytosis and uptake of apoptotic cells
    C A Ogden
    Department of Pediatrics, National Jewish Medical and Research Center, Denver, CO 80206, USA
    J Exp Med 194:781-95. 2001
  5. ncbi DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells
    T B Geijtenbeek
    Department of Tumor Immunology, University Medical Center St Radboud, Nijmegen, The Netherlands
    Cell 100:587-97. 2000
  6. pmc C-type lectins DC-SIGN and L-SIGN mediate cellular entry by Ebola virus in cis and in trans
    Carmen P Alvarez
    Laboratory of Molecular Microbiology, Dept of Microbiology, Hospital 12 de Octubre, 28041 Madrid, Spain
    J Virol 76:6841-4. 2002
  7. ncbi Omentin plasma levels and gene expression are decreased in obesity
    Celia M de Souza Batista
    Division of Endocrinology, Diabetes and Nutrition, Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland, USA
    Diabetes 56:1655-61. 2007
  8. ncbi Galectins. Structure and function of a large family of animal lectins
    S H Barondes
    Department of Psychiatry, University of California, San Francisco 94143
    J Biol Chem 269:20807-10. 1994
  9. pmc Role of YKL-40 in the angiogenesis, radioresistance, and progression of glioblastoma
    Ralph A Francescone
    Molecular and Cellular Biology Program, Morrill Science Center, University of Massachusetts, Amherst, Massachusetts 01003, USA
    J Biol Chem 286:15332-43. 2011
  10. ncbi A chitinase-like protein in the lung and circulation of patients with severe asthma
    Geoffrey L Chupp
    Yale University School of Medicine, New Haven, CT, USA
    N Engl J Med 357:2016-27. 2007

Detail Information

Publications413 found, 100 shown here

  1. ncbi Glycosylation in cellular mechanisms of health and disease
    Kazuaki Ohtsubo
    Howard Hughes Medical Institute and Department of Cellular and Molecular Medicine, 9500 Gilman Drive MC0625, University of California, San Diego, La Jolla, CA 92093, USA
    Cell 126:855-67. 2006
    ..This review discusses the increasingly sophisticated molecular mechanisms being discovered by which mammalian glycosylation governs physiology and contributes to disease...
  2. ncbi Siglecs and their roles in the immune system
    Paul R Crocker
    Wellcome Trust Biocentre, College of Life Sciences, University of Dundee, Dundee DD 15EH, UK
    Nat Rev Immunol 7:255-66. 2007
    ..The Siglecs are a family of sialic-acid-binding immunoglobulin-like lectins that are thought to promote cell-cell interactions and regulate the functions of cells in the innate and adaptive ..
  3. pmc Role of chitin and chitinase/chitinase-like proteins in inflammation, tissue remodeling, and injury
    Chun Geun Lee
    Section of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520 8057, USA
    Annu Rev Physiol 73:479-501. 2011
    ..Our understanding of the roles of C/CLPs in inflammation, tissue remodeling, and tissue injury in health and disease is reviewed below...
  4. pmc C1q and mannose binding lectin engagement of cell surface calreticulin and CD91 initiates macropinocytosis and uptake of apoptotic cells
    C A Ogden
    Department of Pediatrics, National Jewish Medical and Research Center, Denver, CO 80206, USA
    J Exp Med 194:781-95. 2001
    ....
  5. ncbi DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells
    T B Geijtenbeek
    Department of Tumor Immunology, University Medical Center St Radboud, Nijmegen, The Netherlands
    Cell 100:587-97. 2000
    ..We propose that DC-SIGN efficiently captures HIV-1 in the periphery and facilitates its transport to secondary lymphoid organs rich in T cells, to enhance infection in trans of these target cells...
  6. pmc C-type lectins DC-SIGN and L-SIGN mediate cellular entry by Ebola virus in cis and in trans
    Carmen P Alvarez
    Laboratory of Molecular Microbiology, Dept of Microbiology, Hospital 12 de Octubre, 28041 Madrid, Spain
    J Virol 76:6841-4. 2002
    ..Here we show that the primate lentiviral binding C-type lectins DC-SIGN and L-SIGN act as cofactors for cellular entry by Ebola virus...
  7. ncbi Omentin plasma levels and gene expression are decreased in obesity
    Celia M de Souza Batista
    Division of Endocrinology, Diabetes and Nutrition, Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland, USA
    Diabetes 56:1655-61. 2007
    ..In summary, decreased omentin levels are associated with increasing obesity and insulin resistance. Therefore, omentin levels may be predictive of the metabolic consequences or co-morbidities associated with obesity...
  8. ncbi Galectins. Structure and function of a large family of animal lectins
    S H Barondes
    Department of Psychiatry, University of California, San Francisco 94143
    J Biol Chem 269:20807-10. 1994
  9. pmc Role of YKL-40 in the angiogenesis, radioresistance, and progression of glioblastoma
    Ralph A Francescone
    Molecular and Cellular Biology Program, Morrill Science Center, University of Massachusetts, Amherst, Massachusetts 01003, USA
    J Biol Chem 286:15332-43. 2011
    ..These findings have shed light on the mechanisms by which YKL-40 promotes tumor angiogenesis and malignancy, and thus provide a therapeutic target for tumor treatment...
  10. ncbi A chitinase-like protein in the lung and circulation of patients with severe asthma
    Geoffrey L Chupp
    Yale University School of Medicine, New Haven, CT, USA
    N Engl J Med 357:2016-27. 2007
    ..The related chitinase-like protein, YKL-40 (also called human cartilage glycoprotein 39 [HCgp-39] and chitinase 3-like 1), can be readily measured in the serum. However, its relationship to asthma has not been evaluated...
  11. pmc Role of breast regression protein 39 (BRP-39)/chitinase 3-like-1 in Th2 and IL-13-induced tissue responses and apoptosis
    Chun Geun Lee
    Section of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520, USA
    J Exp Med 206:1149-66. 2009
    ..These studies establish novel regulatory roles for BRP-39/YKL-40 in the initiation and effector phases of Th2 inflammation and remodeling and suggest that these proteins are therapeutic targets in Th2- and macrophage-mediated disorders...
  12. ncbi C-type lectin-like domains
    K Drickamer
    Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK
    Curr Opin Struct Biol 9:585-90. 1999
    Carbohydrate-recognition domains of C-type (Ca2+-dependent) animal lectins serve as prototypes for an important family of protein modules. Only some domains in this family bind Ca2+ or sugars...
  13. pmc Extremes of L-ficolin concentration in children with recurrent infections are associated with single nucleotide polymorphisms in the FCN2 gene
    M Cedzynski
    Laboratory of Immunobiology of Infections, Centre of Medical Biology, Polish Academy of Sciences, Lodz, Poland
    Clin Exp Immunol 150:99-104. 2007
    ..Conversely, high l-ficolin levels were associated with variant alleles of ss32469537 and ss32469544. FCN2 genotyping should be a valuable additional tool for disease association studies...
  14. pmc Carbohydrate recognition properties of human ficolins: glycan array screening reveals the sialic acid binding specificity of M-ficolin
    Evelyne Gout
    Laboratoire d Enzymologie Moleculaire, Institut de Biologie Structurale Jean Pierre Ebel, Commissariat a l Energie Atomique, CNRS UMR 5075, Universite Joseph Fourier, 41 rue Jules Horowitz, Grenoble 38027 Cedex 1, France
    J Biol Chem 285:6612-22. 2010
    ....
  15. pmc The mycobacterial heparin-binding hemagglutinin is a protective antigen in the mouse aerosol challenge model of tuberculosis
    Marcela Parra
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bldg 29, Rm 503, HFM 431, 29 Lincoln Drive, Bethesda, MD 20892, USA
    Infect Immun 72:6799-805. 2004
    ..tuberculosis are critical for generating effective host immune responses. The vaccine studies described here demonstrate that HBHA is a promising new vaccine candidate for tuberculosis...
  16. pmc YKL-40, a secreted glycoprotein, promotes tumor angiogenesis
    R Shao
    Pioneer Valley Life Sciences Institute, Springfield, MA 01199, USA
    Oncogene 28:4456-68. 2009
    ..These findings provide novel insights into angiogenic activities and molecular mechanisms of YKL-40 in cancer development...
  17. pmc A YKL-40-neutralizing antibody blocks tumor angiogenesis and progression: a potential therapeutic agent in cancers
    Michael Faibish
    Molecular and Cellular Biology Program, Morrill Science Center, University of Massachusetts, Amherst, MA, USA
    Mol Cancer Ther 10:742-51. 2011
    ..Furthermore, treatment of xenografted tumor mice with mAY restrained tumor growth, angiogenesis, and progression. Taken together, this study has shown the therapeutic use for the mAY in treatment of tumor angiogenesis and metastasis...
  18. pmc Molecular characterization of the mycobacterial heparin-binding hemagglutinin, a mycobacterial adhesin
    F D Menozzi
    Laboratoire de Microbiologie Génétique et Moléculaire, Institut National de la Santé et de la Recherche Médicale U447, Institut Pasteur de Lille, 1 rue Calmette, F 59019 Lille cedex, France
    Proc Natl Acad Sci U S A 95:12625-30. 1998
    ..Finally, the absence of a signal peptide in the coding region of HBHA raises the possibility that this protein is not secreted via the general secretion pathway...
  19. ncbi Molecular characterization of the gene for human cartilage gp-39 (CHI3L1), a member of the chitinase protein family and marker for late stages of macrophage differentiation
    M Rehli
    Department of Hematology and Oncology, University of Regensburg, Germany
    Genomics 43:221-5. 1997
    ..We sequenced 1.3 kb of the 5'-flanking region and identified a variety of putative regulatory elements...
  20. ncbi CNS-derived interleukin-4 is essential for the regulation of autoimmune inflammation and induces a state of alternative activation in microglial cells
    Eugene D Ponomarev
    BloodCenter of Wisconsin, Blood Research Institute, Milwaukee, Wisconsin 53201 2178, USA
    J Neurosci 27:10714-21. 2007
    ..Macrophages that have undergone alternative activation have been shown to be important in tissue repair; thus, our results suggest a new role for microglial cells in the regulation of inflammation in the CNS...
  21. ncbi A macrophage protein, Ym1, transiently expressed during inflammation is a novel mammalian lectin
    N C Chang
    Institute of Microbiology and Immunology, National Yang Ming University, Taipei, Taiwan 112, Republic of China
    J Biol Chem 276:17497-506. 2001
    ..Although it shares approximately 30% homology with microbial chitinases, no chitinase activity was found associated with Ym1. Genomic Southern blot analyses suggest that Ym1 may represent a member of a novel lectin gene family...
  22. ncbi The role of ficolins in the lectin pathway of innate immunity
    Yuichi Endo
    Department of Immunology, Fukushima Medical University School of Medicine, 1 Hikarigaoka, Fukushima 960 1295, Japan
    Int J Biochem Cell Biol 43:705-12. 2011
    ..They are novel lectins that employ the fibrinogen-like domain as a functional domain...
  23. ncbi The impact of FCN2 polymorphisms and haplotypes on the Ficolin-2 serum levels
    L Munthe-Fog
    Tissue Typing Laboratory, Department of Clinical Immunology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark
    Scand J Immunol 65:383-92. 2007
    ..Our results show that inter-individual variation of Ficolin-2 concentration is associated with polymorphisms in the promoter and the structural part of the FCN2 gene...
  24. ncbi Glycoprotein folding, quality control and ER-associated degradation
    Gerardo Z Lederkremer
    Department of Cell Research and Immunology, George Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel
    Curr Opin Struct Biol 19:515-23. 2009
    ..Recent studies help understand the code displayed by each structure produced by this trimming and its decoding by lectins. The calnexin folding cycle targets only monoglucosylated oligosaccharides...
  25. ncbi Ficolin-2 levels and FCN2 genetic polymorphisms as a susceptibility factor in schistosomiasis
    Eman Abou Ouf
    Institute of Tropical Medicine, University of Tubingen, Germany
    J Infect Dis 206:562-70. 2012
    ..Human ficolin-2 (L-ficolins) encoded by the FCN2 gene are pattern-recognition proteins involved in innate immunity and are associated with several infectious diseases...
  26. pmc Burkholderia cenocepacia BC2L-C is a super lectin with dual specificity and proinflammatory activity
    Ondrej Sulák
    CERMAV CNRS UPR5301 affiliated to Université Joseph Fourier, Grenoble, France
    PLoS Pathog 7:e1002238. 2011
    b>Lectins and adhesins are involved in bacterial adhesion to host tissues and mucus during early steps of infection...
  27. ncbi Expression of H-ficolin/Hakata antigen, mannose-binding lectin-associated serine protease (MASP)-1 and MASP-3 by human glioma cell line T98G
    Mikio Kuraya
    Department of Biochemistry, Fukushima Medical University School of Medicine, 1 Hikarigaoka, Fukushima 960 1295, Japan
    Int Immunol 15:109-17. 2003
    ..Since the glioma cells used are derived from astrocytes, this suggests that human astrocytes may be a source of some components of the LCP in the brain...
  28. ncbi Mannose-binding lectin levels during pregnancy: a longitudinal study
    F E van de Geijn
    Department of Rheumatology, Erasmus MC University Medical Center Rotterdam, Rotterdam, The Netherlands
    Hum Reprod 22:362-71. 2007
    ..Therefore, we aimed to determine whether MBL concentrations and the following steps in complement pathway activation are influenced by pregnancy...
  29. pmc OS-9 and GRP94 deliver mutant alpha1-antitrypsin to the Hrd1-SEL1L ubiquitin ligase complex for ERAD
    John C Christianson
    Department of Biological Sciences and Bio X Program, Stanford University, Lorry Lokey Bldg, 337 Campus Drive, Stanford, CA 94305, USA
    Nat Cell Biol 10:272-82. 2008
    ..These data suggest that XTP3-B and OS-9 are components of distinct, partially redundant, quality control surveillance pathways that coordinate protein folding with membrane dislocation and ubiquitin conjugation in mammalian cells...
  30. pmc Alpha-(1-3)- and alpha-(1-6)-D-mannose-specific plant lectins are markedly inhibitory to human immunodeficiency virus and cytomegalovirus infections in vitro
    J Balzarini
    Laboratory of Chemotherapy, Rega Institute for Medical Research, Katholieke Universiteit Leuven, Belgium
    Antimicrob Agents Chemother 35:410-6. 1991
    The alpha-(1-3)-D-mannose- and alpha-(1-6)-D-mannose-specific agglutinins (lectins) from Galanthus nivalis, Hippeastrum hybrid, Narcissus pseudonarcissus, and Listera ovata inhibited infection of MT-4 cells by human immunodeficiency ..
  31. ncbi Identification of DC-SIGN, a novel dendritic cell-specific ICAM-3 receptor that supports primary immune responses
    T B Geijtenbeek
    Department of Tumor Immunology, University Medical Center St Radboud, Nijmegen, The Netherlands
    Cell 100:575-85. 2000
    ..Since antibodies against DC-SIGN inhibit DC-induced proliferation of resting T cells, our findings predict that DC-SIGN enables T cell receptor engagement by stabilization of the DC-T cell contact zone...
  32. ncbi A novel human serum lectin with collagen- and fibrinogen-like domains that functions as an opsonin
    M Matsushita
    Department of Biochemistry, Fukushima Medical College, Japan
    J Biol Chem 271:2448-54. 1996
    Collectins are C-type animal lectins with both collagenous and carbohydrate recognition domains and are involved in the first line host defense against pathogens...
  33. pmc Human OS-9, a lectin required for glycoprotein endoplasmic reticulum-associated degradation, recognizes mannose-trimmed N-glycans
    Nobuko Hosokawa
    Department of Molecular and Cellular Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8397, Japan
    J Biol Chem 284:17061-8. 2009
    In the endoplasmic reticulum (ER), lectins and processing enzymes are involved in quality control of newly synthesized proteins for productive folding as well as in the ER-associated degradation (ERAD) of misfolded proteins...
  34. pmc Synergy between ficolin-2 and pentraxin 3 boosts innate immune recognition and complement deposition
    Ying Jie Ma
    Laboratory of Molecular Medicine, Department of Clinical Immunology, Section 7631, Rigshospitalet, Faculty of Health Sciences, University of Copenhagen, Copenhagen 2100, Denmark
    J Biol Chem 284:28263-75. 2009
    ..Thus, components of the humoral innate immune system, which activate different complement pathways, cooperate and amplify microbial recognition and effector functions...
  35. pmc C-reactive protein collaborates with plasma lectins to boost immune response against bacteria
    Patricia M L Ng
    Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore
    EMBO J 26:3431-40. 2007
    ..We propose that CRP does not act alone but collaborates with other plasma PRRs to form stable pathogen recognition complexes when targeting a wide range of bacteria for destruction...
  36. ncbi Elevated pretreatment serum concentration of YKL-40-An independent prognostic biomarker for poor survival in patients with metastatic nonsmall cell lung cancer
    Ina Thom
    Department of Oncology, Hematology, and Bone Marrow Transplantation with Section Pneumology, Hubertus Wald Tumor Center, University Cancer Center Hamburg, University Medical Center Hamburg Eppendorf, Hamburg, Germany
    Cancer 116:4114-21. 2010
    ..Consequently, high serum YKL-40 levels have been associated with a poor prognosis in patients with several cancer types. However, the role of YKL-40 has not been established in nonsmall cell lung cancer (NSCLC)...
  37. pmc Molecular mimicry of host sialylated glycans allows a bacterial pathogen to engage neutrophil Siglec-9 and dampen the innate immune response
    Aaron F Carlin
    Department of Medicine, Pediatrics, and Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093 0687, USA
    Blood 113:3333-6. 2009
    ..Thus, GBS can impair neutrophil defense functions by coopting a host inhibitory receptor via sialoglycan molecular mimicry, a novel mechanism of bacterial immune evasion...
  38. pmc A glycobiology review: carbohydrates, lectins and implications in cancer therapeutics
    Haike Ghazarian
    Department of Biology and Center for Cancer and Developmental Biology, California State University Northridge, 18111 Nordhoff Street, Northridge, CA 91330 8303, USA
    Acta Histochem 113:236-47. 2011
    ..These topics are among the hundreds included in the field of glycobiology and are treated here because they form the cornerstone of glycobiology or the focus of many advances in this rapidly expanding field...
  39. ncbi YKL-40 is elevated in patients with chronic obstructive pulmonary disease and activates alveolar macrophages
    Severine Letuve
    Institut National de la Santé et de la Recherche Médicale Unité 700, Physiopathology and Epidemiology of Respiratory Insufficiency, Paris, France
    J Immunol 181:5167-73. 2008
    ..We conclude that YKL-40 is up-regulated in COPD, in which it may contribute to tissue inflammation and remodeling by sustaining the synthesis of proinflammatory and fibrogenic chemokines and of metalloproteinases by alveolar macrophages...
  40. ncbi Omentin, a novel adipokine, induces vasodilation in rat isolated blood vessels
    Hideyuki Yamawaki
    Department of Veterinary Pharmacology, School of Veterinary Medicine, Kitasato University, Towada City, Aomori 034 8628, Japan
    Biochem Biophys Res Commun 393:668-72. 2010
    ..Omentin (1-300 ng/ml) also relaxed NA pre-contracted mesenteric artery. Present study for the first time demonstrated that omentin has a vasodilating effect on isolated blood vessels, which is mediated through endothelium-derived NO...
  41. pmc Genetic evidence of functional ficolin-2 haplotype as susceptibility factor in cutaneous leishmaniasis
    Amal Assaf
    Institute of Tropical Medicine, University of Tubingen, Wilhelmstr 27, Tubingen, Germany
    PLoS ONE 7:e34113. 2012
    ..Ficolin-2 coded by FCN2 gene is a soluble serum protein that plays an important role in innate immunity. In this study, we analyzed five functional polymorphisms of the FCN2 gene for their possible association with cutaneous leishmaniasis...
  42. ncbi Lectin complement pathway gene profile of donor and recipient determine the risk of bacterial infections after orthotopic liver transplantation
    Bert Jan F de Rooij
    Department of Gastroenterology and Hepatology, Leiden University Medical Center, Leiden, The Netherlands
    Hepatology 52:1100-10. 2010
    ..7 and P < 0.02). Moreover, patients with a lectin pathway gene polymorphism and infection had a six-fold higher mortality (P = 0.9 x 10(-8)), of which 80% was infection-related...
  43. ncbi P-type lectins
    Nancy M Dahms
    Department of Biochemistry, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Biochim Biophys Acta 1572:317-40. 2002
    ..and the insulin-like growth factor II/mannose 6-phosphate receptor (IGF-II/MPR), are distinguished from all other lectins by their ability to recognize phosphorylated mannose residues...
  44. ncbi A strategy for discovery of cancer glyco-biomarkers in serum using newly developed technologies for glycoproteomics
    Hisashi Narimatsu
    Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan
    FEBS J 277:95-105. 2010
    ..to predict the glycan structures of secreted glycoproteins; (b) analysis by lectin microarray to select lectins that distinguish cancer-related glycan structures on secreted glycoproteins; and (c) an isotope-coded ..
  45. ncbi The membrane-proximal immunoreceptor tyrosine-based inhibitory motif is critical for the inhibitory signaling mediated by Siglecs-7 and -9, CD33-related Siglecs expressed on human monocytes and NK cells
    Tony Avril
    Division of Cell Biology and Immunology, The Wellcome Trust Biocentre, University of Dundee, Dundee, United Kingdom
    J Immunol 173:6841-9. 2004
    ..Finally, mutation of the membrane-proximal motif increased the sialic acid binding activity of Siglecs-7 and -9, raising the possibility that "inside-out" signaling may occur to regulate ligand binding...
  46. ncbi Revisiting proteus: do minor changes in lectin structure matter in biological activity? Lessons from and potential biotechnological uses of the Diocleinae subtribe lectins
    B S Cavada
    BioMol Lab, Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, CEP 60 451 970 Fortaleza, Brasil
    Curr Protein Pept Sci 2:123-35. 2001
    Significant differences in function have been observed among lectins structurally similar to concanavalin A, but their high homology with this widely used lectin has kept them in obscurity...
  47. ncbi Human cartilage gp-39, a major secretory product of articular chondrocytes and synovial cells, is a mammalian member of a chitinase protein family
    B E Hakala
    Joint Diseases Laboratory, Shriners Hospital for Crippled Children, Montreal, Quebec, Canada
    J Biol Chem 268:25803-10. 1993
    ..These observations suggest that the expression of HC gp-39 may be related to a response of these cells to an altered tissue environment...
  48. pmc A novel L-ficolin/mannose-binding lectin chimeric molecule with enhanced activity against Ebola virus
    Ian C Michelow
    From the Program of Developmental Immunology, Department of Pediatrics, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114
    J Biol Chem 285:24729-39. 2010
    ..L-FCN/MBL chimeric proteins should be considered as potential novel therapeutics...
  49. pmc Recombinant chimeric lectins consisting of mannose-binding lectin and L-ficolin are potent inhibitors of influenza A virus compared with mannose-binding lectin
    Wei Chuan Chang
    Program of Developmental Immunology, Department of Pediatrics, Massachusetts General Hospital, Harvard Medical School, 55 Fruit Street, Boston, MA 02114, USA
    Biochem Pharmacol 81:388-95. 2011
    ..We have recently generated three novel recombinant chimeric lectins (RCL), in which varying length of collagenous domain of mannose-binding lectin (MBL) is replaced with that of L-..
  50. pmc No strong relationship between mannan binding lectin or plasma ficolins and chemotherapy-related infections
    D C Kilpatrick
    SNBTS National Science Laboratory, University of Edinburgh, UK
    Clin Exp Immunol 134:279-84. 2003
    ..These results cast doubt on the potential value of MBL replacement therapy in this clinical context...
  51. ncbi Two factors of the lectin pathway of complement, l-ficolin and mannan-binding lectin, and their associations with prematurity, low birthweight and infections in a large cohort of Polish neonates
    Anna St Swierzko
    Laboratory of Immunobiology of Infections, Institute of Medical Biology, Polish Academy of Sciences, Lodowa 106, 93 232 Lodz, Poland
    Mol Immunol 46:551-8. 2009
    ..Some similar trends were found with facets of MBL deficiency, but the observed relationships were weaker and less consistent...
  52. pmc Binding of the mannose-specific lectin, griffithsin, to HIV-1 gp120 exposes the CD4-binding site
    Kabamba Bankoledi Alexandre
    AIDS Virus Research Unit, National Institute for Communicable Diseases, Johannesburg, Private Bag X4, Sandringham 2131, Johannesburg, South Africa
    J Virol 85:9039-50. 2011
    ..They also serve as targets for lectins that bind mannose-rich glycans...
  53. ncbi Genomic structure of human omentin, a new adipocytokine expressed in omental adipose tissue
    A Schaffler
    Department of Internal Medicine I, University of Regensburg, D 93042 Regensburg, Germany
    Biochim Biophys Acta 1732:96-102. 2005
    ..Based on this, omentin might be a new adipocytokine playing a role in the defense against intestinal bacterial translocation in the context of Crohn's disease...
  54. pmc A novel mannose-binding lectin/ficolin-associated protein is highly expressed in heart and skeletal muscle tissues and inhibits complement activation
    Mikkel Ole Skjoedt
    Laboratory of Molecular Medicine, Department of Clinical Immunology, Rigshospitalet, Faculty of Health Sciences, University Hospital of Copenhagen, DK 2100 Copenhagen, Denmark
    J Biol Chem 285:8234-43. 2010
    ..It is found in complex with MBL and ficolins and may function as a potent inhibitor of the complement system in vivo...
  55. pmc Essential role of mannose-binding lectin-associated serine protease-1 in activation of the complement factor D
    Minoru Takahashi
    Department of Immunology, Institute of Biomedical Sciences, Fukushima Medical University School of Medicine, Fukushima 960 1295, Japan
    J Exp Med 207:29-37. 2010
    ..Thus, it is clear that MASP-1 is an essential protease of both the lectin and alternative complement pathways...
  56. ncbi Lectin microarray analysis of pluripotent and multipotent stem cells
    Masashi Toyoda
    Department of Reproductive Biology, National Institute for Child Health and Development, 2 10 1 Okura, Setagaya Ku, Tokyo 157 8535, Japan
    Genes Cells 16:1-11. 2011
    ..The pair-wise comparison means based on 'false discovery rate' revealed that three lectins -Euonymus europaeus lectin (EEL), Maackia amurensis lectin (MAL) and Phaseolus vulgaris leucoagglutinin [PHA(L)]- ..
  57. pmc Members of a novel protein family containing microneme adhesive repeat domains act as sialic acid-binding lectins during host cell invasion by apicomplexan parasites
    Nikolas Friedrich
    Department of Microbiology and Molecular Medicine, Faculty of Medicine, University of Geneva Centre Medical Universitaire, 1 rue Michel Servet, 1211 Geneva 4, Switzerland
    J Biol Chem 285:2064-76. 2010
    ..However, the three lectins also display differences in binding preferences...
  58. ncbi Ficolin-2 recognizes DNA and participates in the clearance of dying host cells
    Maria Lund Jensen
    Department of Clinical Immunology, Rigshospitalet, Copenhagen University Hospital, Blegdamsvej 9, DK 2100 Copenhagen O, Denmark
    Mol Immunol 44:856-65. 2007
    ..Ficolin-2 may therefore be a scavenger molecule participating in the removal of host cells and maintenance of tissue homeostasis...
  59. pmc Role of LecA and LecB lectins in Pseudomonas aeruginosa-induced lung injury and effect of carbohydrate ligands
    Chanez Chemani
    EA 2689, Faculte de Medecine, 1 place de Verdun, F 59045 Lille Cedex, France
    Infect Immun 77:2065-75. 2009
    ..Our results demonstrate that there is a relationship between lectins and the pathogenicity of P. aeruginosa...
  60. ncbi Plasma YKL-40: a potential new cancer biomarker?
    Julia S Johansen
    Department of Medicine O, Herlev Hospital, Herlev Ringvej 75, DK 2730, Herlev, Denmark
    Future Oncol 5:1065-82. 2009
    ..Large prospective, longitudinal clinical cancer studies are needed to determine if plasma YKL-40 is a new cancer biomarker, or is mainly a biomarker of inflammation...
  61. pmc The decreased expression of Siglec-7 represents an early marker of dysfunctional natural killer-cell subsets associated with high levels of HIV-1 viremia
    Enrico Brunetta
    Laboratory of Clinical and Experimental Immunology, Istituto di Ricovero e Cura a Carattere Scientifico, Istituto Clinico Humanitas, Rozzano, Milano, Italy
    Blood 114:3822-30. 2009
    ..High frequencies of Siglec-7-/CD56+ and Siglec-7-/CD56- pathologic NK cells reflect the immune and clinical status of HIV-1 infection and can also track the effectiveness of therapy...
  62. pmc Stringent requirement for HRD1, SEL1L, and OS-9/XTP3-B for disposal of ERAD-LS substrates
    Riccardo Bernasconi
    Institute for Research in Biomedicine, 6500 Bellinzona, Switzerland
    J Cell Biol 188:223-35. 2010
    ..dependent on the E3 ubiquitin ligase HRD1, the associated cargo receptor SEL1L, and two interchangeable ERAD lectins, OS-9 and XTP3-B...
  63. ncbi Isolation and characterization of griffithsin, a novel HIV-inactivating protein, from the red alga Griffithsia sp
    Toshiyuki Mori
    Molecular Targets Development Program, Center for Cancer Research, NCI Frederick, Maryland 21702, USA
    J Biol Chem 280:9345-53. 2005
    ..GRFT could be a potential candidate microbicide to prevent the sexual transmission of HIV and AIDS...
  64. pmc Heparin-binding-hemagglutinin-induced IFN-gamma release as a diagnostic tool for latent tuberculosis
    Jean Michel Hougardy
    Laboratory of Vaccinology and Mucosal Immunity, Hopital Erasme, Universite Libre de Bruxelles, Brussels, Belgium
    PLoS ONE 2:e926. 2007
    ..We have therefore evaluated the potential of the novel antigen heparin-binding hemagglutinin (HBHA) for in vitro detection of LTBI...
  65. ncbi The heparin-binding haemagglutinin of M. tuberculosis is required for extrapulmonary dissemination
    K Pethe
    Unité INSERM U447, Institut Pasteur de Lille, 1 rue du Prof Calmette, F 59019 Lille cedex, France
    Nature 412:190-4. 2001
    ..They also suggest that antibody responses to HBHA may add to immune protection against tuberculosis...
  66. ncbi Polymorphisms in the FCN2 gene determine serum variation and function of Ficolin-2
    Tina Hummelshoj
    Department of Clinical Immunology, Tissue Typing Laboratory 7631, Rigshospitalet, 2100 Copenhagen, Denmark
    Hum Mol Genet 14:1651-8. 2005
    ....
  67. ncbi Glomerular activation of the lectin pathway of complement in IgA nephropathy is associated with more severe renal disease
    Anja Roos
    Department of Clinical Chemistry, Leiden University Medical Center, The Netherlands, and Fonazione D Amico per la Ricerca sulle Malattie Renali, Associazione Nuova Nefrologia, San Carlo Borromeo Hospital, Milano, Italy
    J Am Soc Nephrol 17:1724-34. 2006
    ..Together, these findings strongly point to a role for the lectin pathway of complement in glomerular complement activation in IgAN and suggest a contribution for both MBL and L-ficolin in the progression of the disease...
  68. ncbi The innate pattern recognition molecule Ficolin-1 is secreted by monocytes/macrophages and is circulating in human plasma
    Christian Honore
    Department of Clinical Immunology, Rigshospitalet, Blegdamsvej 9, Copenhagen, Denmark
    Mol Immunol 45:2782-9. 2008
    ..Importantly, these results demonstrate that Ficolin-1 exists in human plasma and serum under normal conditions, hereby revising the general assumption that Ficolin-1 is solely a cellular associated protein...
  69. ncbi Immunodeficiency associated with FCN3 mutation and ficolin-3 deficiency
    Lea Munthe-Fog
    Laboratory of Molecular Medicine, Department of Clinical Immunology, Sect 7631, Rigshospitalet, University of Copenhagen, Faculty of Health Sciences, Copenhagen, Denmark
    N Engl J Med 360:2637-44. 2009
    ..01). We describe a patient with recurrent infections who was homozygous for this mutation, who had undetectable serum levels of ficolin-3, and who had a deficiency in ficolin-3-dependent complement activation...
  70. ncbi Cloning and characterization of the human lectin P35 gene and its related gene
    Y Endo
    Department of Biochemistry, Fukushima Medical College, Japan
    Genomics 36:515-21. 1996
    ..Both genes were assigned to a closely related region of chromosome 9 at 9q34...
  71. ncbi Use of glycan microarrays to explore specificity of glycan-binding proteins
    David F Smith
    Department of Biochemistry, The Glycomics Center, Emory University School of Medicine, Atlanta, Georgia, USA
    Methods Enzymol 480:417-44. 2010
    Microarrays of defined glycans represent a high throughput approach to determining the specificity of lectins, or more generally glycan-binding proteins (GBPs)...
  72. ncbi MBL2, FCN1, FCN2 and FCN3-The genes behind the initiation of the lectin pathway of complement
    Peter Garred
    Department of Clinical Immunology, Rigshospitalet, University of Copenhagen, Denmark
    Mol Immunol 46:2737-44. 2009
    ..This overview will summarize the current molecular knowledge of the human MBL2, FCN1, FCN2 and FCN3 genes...
  73. ncbi Serum concentrations of lectin-pathway components in healthy neonates, children and adults: mannan-binding lectin (MBL), M-, L-, and H-ficolin, and MBL-associated serine protease-2 (MASP-2)
    Seraina Sallenbach
    Department of Pediatrics, University of Bern, Bern, Switzerland
    Pediatr Allergy Immunol 22:424-30. 2011
    ..The respective normal ranges for adults should not be used in the pediatric population. The age-specific pediatric ranges established here may be used instead...
  74. pmc Ficolin-2 levels and FCN2 haplotypes influence hepatitis B infection outcome in Vietnamese patients
    Tong V Hoang
    Institute of Tropical Medicine, University of Tubingen, Tubingen, Germany
    PLoS ONE 6:e28113. 2011
    ..Strong linkage was found between the variant -986G>A and -4A>G. The very first study on Vietnamese cohort associates both Ficolin-2 serum levels and FCN2 haplotypes to hepatitis B virus infection and subsequent disease progression...
  75. ncbi TH2 cytokines and allergic challenge induce Ym1 expression in macrophages by a STAT6-dependent mechanism
    John S Welch
    Department of Cellular and Molecular Medicine, La Jolla, California 92093 0651, USA
    J Biol Chem 277:42821-9. 2002
    ..These studies establish Ym1 as a highly inducible STAT6-dependent transcript in T(H)2-biased inflammation and define Cis-active elements in the Ym1 promoter that are required for this transcriptional response...
  76. ncbi Roles of N-linked glycans in the endoplasmic reticulum
    Ari Helenius
    Institute of Biochemistry1 Swiss Federal Institute of Technology Zurich, Zurich 8093, Switzerland
    Annu Rev Biochem 73:1019-49. 2004
    ..but also indirectly by serving as recognition "tags" that allow glycoproteins to interact with a variety of lectins, glycosidases, and glycosyltranferases...
  77. ncbi Sialic acid-specific lectins: occurrence, specificity and function
    F Lehmann
    Institute for Glycomics, Griffith University, Gold Coast Campus, PMB 50, Gold Coast Mail Centre, 9726 Australia
    Cell Mol Life Sci 63:1331-54. 2006
    ..involves the direct participation of sialic acids in recognition events through specific interactions with lectins, a family of proteins that recognise and bind sugars...
  78. ncbi Molecular interaction of Siglecs (sialic acid-binding Ig-like lectins) with sialylated ligands on Trypanosoma cruzi
    Thomas Jacobs
    Department of Immunology, Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht Strasse 74, 20359 Hamburg, Germany
    Eur J Cell Biol 89:113-6. 2010
    ..cruzi with a special emphasis on their property to interact with sialic acid-binding Ig-like lectins, which may allow the parasite to modulate the immune system of the host.
  79. pmc Specifically binding of L-ficolin to N-glycans of HCV envelope glycoproteins E1 and E2 leads to complement activation
    Jun Liu
    The State Key Laboratory of Virology, Department of Immunology and Hubei Province Key Laboratory of Allergy and Immune related Diseases, Wuhan University School of Medicine, Wuhan 430071, China
    Cell Mol Immunol 6:235-44. 2009
    ..These findings provide new insights into the biological functions of L-ficolin in clinically important hepatic viral diseases...
  80. ncbi Role of early lectin pathway activation in the complement-mediated killing of Trypanosoma cruzi
    Igor Dos S Cestari
    Instituto Oswaldo Cruz FIOCRUZ, Laboratório de Biologia Molecular de Parasitas e Vetores, Rio de Janeiro, 21040 900, Brazil
    Mol Immunol 47:426-37. 2009
    ..Our findings establish that the complement lectin pathway recognizes T. cruzi and provide molecular insights into how the infectious stage inhibits this activation to resist complement system killing...
  81. ncbi Ficolin-2 levels and genetic polymorphisms of FCN2 in malaria
    Imad Faik
    Institute for Tropical Medicine, University of Tubingen, Tubingen, Germany
    Hum Immunol 72:74-9. 2011
    ..No FCN2 promoter haplotypes were significantly distributed between mild and severe cases...
  82. pmc Ficolin 2 (FCN2) functional polymorphisms and the risk of rheumatic fever and rheumatic heart disease
    I J Messias-Reason
    Department of Parasitology, Institute of Tropical Medicine, University of Tubingen, 72074 Tubingen, Germany
    Clin Exp Immunol 157:395-9. 2009
    ..5%, OR 3.2, CI 95% 0.13-0.77, P = 0.008). Based on those findings, one can conclude that polymorphisms associated with low levels of L-ficolin level may predispose an individual to recurrent and/or more severe streptococcal infection...
  83. ncbi Inhibition and dispersion of Pseudomonas aeruginosa biofilms by glycopeptide dendrimers targeting the fucose-specific lectin LecB
    Emma M V Johansson
    Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, CH 3012 Berne, Switzerland
    Chem Biol 15:1249-57. 2008
    ..These experiments suggest that LecB inhibition by high-affinity multivalent ligands could represent a therapeutic approach against P. aeruginosa infections by inhibition of biofilm formation and dispersion of established biofilms...
  84. pmc A dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN)-related protein is highly expressed on human liver sinusoidal endothelial cells and promotes HIV-1 infection
    A A Bashirova
    Laboratory of Genomic Diversity, Science Applications International Corporation Frederick, Frederick, MD 21702, USA
    J Exp Med 193:671-8. 2001
    ..We propose that L-SIGN may play an important role in the interaction between liver sinusoidal endothelium and trafficking lymphocytes, as well as function in the pathogenesis of HIV-1...
  85. ncbi Oligosaccharide specificity of galectins: a search by frontal affinity chromatography
    Jun Hirabayashi
    Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Teikyo University, Sagamiko, Kanagawa 199 0195, Japan
    Biochim Biophys Acta 1572:232-54. 2002
    ..On these bases, they are considered to exert specialized functions in diverse biological phenomena, which may include formation of local cell-surface microdomains (raft) by sorting glycoconjugate members for each cell type...
  86. pmc Structural insights into the innate immune recognition specificities of L- and H-ficolins
    Virginie Garlatti
    Laboratoire de Cristallographie et Cristallogenese des Proteines, Grenoble, France
    EMBO J 26:623-33. 2007
    ....
  87. ncbi Evolution of the lectin-complement pathway and its role in innate immunity
    Teizo Fujita
    Department of Biochemistry, Fukushima Medical University School of Medicine, Japan
    Nat Rev Immunol 2:346-53. 2002
    Discrimination between self and non-self by lectins (carbohydrate-binding proteins) is a strategy of innate immunity that is found in both vertebrates and invertebrates...
  88. ncbi Functional haplotypes that produce normal ficolin-2 levels protect against clinical leprosy
    Iara de Messias-Reason
    Department of Parasitology, Institute for Tropical Medicine, University of Tubingen, Germany
    J Infect Dis 199:801-4. 2009
    ..The distribution of functional FCN2 haplotypes amongpatients was significant different from that among the control subjects (P = .004). These results unveil an immunogenetic role for ficolin-2 in the host response against M. leprae...
  89. pmc Effect of capsulation of opportunistic pathogenic bacteria on binding of the pattern recognition molecules mannan-binding lectin, L-ficolin, and H-ficolin
    Anders Krarup
    Department of Medical Microbiology and Immunology, The Bartholin Building, University of Aarhus, DK 8000 Aarhus C, Denmark
    Infect Immun 73:1052-60. 2005
    ..The concentrations of the three proteins in 97 plasma samples were estimated. The median concentrations were 0.8 mug per ml for MBL, 3.3 mug per ml for L-ficolin, and 18.4 mug per ml for H-ficolin...
  90. pmc Identification of a heparin-binding hemagglutinin present in mycobacteria
    F D Menozzi
    Laboratoire de Microbiologie Genetique et Moléculare, Institut National de la Santé et de la Recherche Médicale U447, Institut Pasteur de Lille, France
    J Exp Med 184:993-1001. 1996
    ..Identification of putative mycobacterial adhesins, such as the one described in this report, may provide the basis for the development of new therapeutic and prophylactic strategies against mycobacterial diseases...
  91. ncbi L-ficolin is a pattern recognition molecule specific for acetyl groups
    Anders Krarup
    Institute of Medical Microbiology and Immunology, University of Aarhus, 8000 Aarhus, Denmark
    J Biol Chem 279:47513-9. 2004
    ..The column was loaded in the presence of EDTA and high salt, and L-ficolin was eluted by decreasing the salt concentration. Further purification was achieved by ion exchange chromatography...
  92. ncbi The genetics of ficolins
    Peter Garred
    Laboratory of Molecular Medicine, Department of Clinical Immunology, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark garred post5 tele dk
    J Innate Immun 2:3-16. 2010
    ..This FCN3 variation was also shown to be associated with immunodeficiency. This survey summarizes the current phylogenetic and inter-individual molecular understanding of the FCN genes...
  93. ncbi Functional SNPs in the human ficolin (FCN) genes reveal distinct geographical patterns
    Tina Hummelshøj
    Department of Clinical Immunology, University of Copenhagen, Denmark
    Mol Immunol 45:2508-20. 2008
    ....
  94. pmc Carbohydrate recognition by boronolectins, small molecules, and lectins
    Shan Jin
    Department of Chemistry and Center for Biotechnology and Drug Design, Georgia State University, Atlanta, Georgia 30302 4098, USA
    Med Res Rev 30:171-257. 2010
    ..This review discusses systematically the general approaches that are available in developing carbohydrate sensors and "binders/receptors," and their applications. The focus is on discoveries during the last 5 years...
  95. ncbi A novel strategy for mammalian cell surface glycome profiling using lectin microarray
    Hiroaki Tateno
    Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, Central 2, 1 1 1 Umezono, Ibaraki 305 8568, Japan
    Glycobiology 17:1138-46. 2007
    ..live cells were applied in situ to the established lectin microarray consisting of 43 immobilized lectins with distinctive binding specificities...
  96. ncbi Experimental anti-angiogenesis causes upregulation of genes associated with poor survival in glioblastoma
    Ahlame Saidi
    ELAT European Laboratory for Angiogenesis and Translational Research, INSERM U920 ex E0113, Talence, F 33405, France
    Int J Cancer 122:2187-98. 2008
    ..In particular, CHI3L1 and PI3/elafin may be useful as new prognostic markers and new therapeutic targets...
  97. ncbi Polymorphisms in the lectin pathway genes as a possible cause of early chronic Pseudomonas aeruginosa colonization in cystic fibrosis patients
    F Haerynck
    Department of Pediatric Pulmonology and Immunology, Ghent University Hospital Ghent, Gent, Belgium
    Hum Immunol 73:1175-83. 2012
    ..Variants in MBL2, FCN1, FCN2 and MASP3 genes are significantly associated with earlier onset of chronic P. aeruginosa colonization...
  98. pmc Porcine arterivirus infection of alveolar macrophages is mediated by sialic acid on the virus
    Peter L Delputte
    Department of Virology, Faculty of Veterinary Medicine, Belgium
    J Virol 78:8094-101. 2004
    ..Studies with linkage-specific neuraminidases and lectins indicated that alpha2-3- and alpha2-6-linked sialic acids on the virion are important for PRRSV infection of PAM...
  99. pmc YKL-40 tissue expression and plasma levels in patients with ovarian cancer
    Estrid V S Høgdall
    Department of Virus, Hormones and Cancer, Institute of Cancer Epidemiology, Danish Cancer Society, Copenhagen, Denmark
    BMC Cancer 9:8. 2009
    ..The aim of the study was to determine the expression of YKL-40 in tumor tissue and plasma in patients with borderline ovarian tumor or epithelial ovarian cancer (OC), and investigate prognostic value of this marker...
  100. ncbi Binding of porcine ficolin-alpha to lipopolysaccharides from Gram-negative bacteria and lipoteichoic acids from Gram-positive bacteria
    Abu M Nahid
    Laboratory of Veterinary Microbiology, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, 1 1 Gakuen cho, Sakai, Osaka 599 8531, Japan
    Dev Comp Immunol 30:335-43. 2006
    ..From the present findings, purified porcine serum protein reactive with GlcNAc is concluded to be ficolin-alpha playing an important role(s) in innate immunity against microbial infection with Gram-positive and -negative bacteria...
  101. ncbi Ficolins: novel pattern recognition molecules of the innate immune response
    Valeria L Runza
    Institute of Immunology, University of Regensburg, Franz Josef Strauss Allee 11, 93042 Regensburg, Germany
    Immunobiology 213:297-306. 2008
    ..When comparing ficolin orthologues from different species, it appears evident that human, murine, and porcine ficolins differ in many aspects, a specific point that we aim to address in this review...

Research Grants72

  1. Targeting Galectin-3 with novel drugs for treatment of eczema
    Fu Tong Liu; Fiscal Year: 2010
    ..However, safety issues raised by the FDA may limit use of these immunomodulators. A family of animal lectins, called galectins, is beta-galactoside-binding animal lectins with conserved carbohydrate-recognition domains...
  2. Induction and maintenance of regulatory T cells
    Mitchell Kronenberg; Fiscal Year: 2013
    ..In Project 4 led by Dr. Croft, we will examine the lectins Galectins-3 and -9, which we have shown are novel ligands for the TNF family receptor 4-1 BB...
  3. Inhibiting Mucosal HIV-1 Transmission by Host Cell RNA Interference
    Alexandra L Howell; Fiscal Year: 2013
    ..In addition, DC can capture HIV-1 via viral binding to C type lectins including DC-SIGN (DC-specific ICAM-grabbing non-integrin)...
  4. Characterization of the ER associated Biogenesis and Degradation of ENaC
    Teresa M Buck; Fiscal Year: 2013
    ..also assay the role of the nucleotide exchange factors Sil1 and Lhs1, as well as the quality control associated lectins in ENaC degradation...
  5. Targeting Siglec-8/-F to treat eosinophil and mast cell related disorders
    Bruce S Bochner; Fiscal Year: 2013
    ..Siglecs (sialic acid-binding, immunoglobulin-like lectins) are cell surface proteins found predominantly on leukocytes...
  6. Targeting Siglec-8/-F to treat eosinophil and mast cell related disorders
    Bruce S Bochner; Fiscal Year: 2012
    ..Siglecs (sialic acid-binding, immunoglobulin-like lectins) are cell surface proteins found predominantly on leukocytes...
  7. Galectin-3 in regulation of allergic skin inflammation
    Fu Tong Liu; Fiscal Year: 2013
    ..Galectin-3 is a member of a family of animal lectins defined by their affinity for 2-galactosides and consensus sequences...
  8. Nanotheranostics for Early Colorectal Cancer Detection and Treatment
    Jeffrey S Souris; Fiscal Year: 2013
    ..releasable NSAIDs (sulindac sulfide, celecoxib, or acetylsalicylic acid) and whose exteriors are covered with lectins/aptamers/peptides that target M-T stage-dependent, aberrant polyp glycan/glycoprotein expression (mucins MUC1 and ..
  9. C3, CR1, CRRY: INTERACTIONS, HOMEOSTASIS AND TRANSLATIONAL IMPLICATIONS
    JOHN PATTERSON ATKINSON; Fiscal Year: 2013
    ..Through natural Abs, lectins and the alternative pathway (AP), especially the AP's feedback loop, the complement system activates on ..
  10. Role of Surfactant in Innate and Adaptive Immunity
    Jo Rae Wright; Fiscal Year: 2010
    ..identified two of the surfactant proteins, SP-A and SP-D, as members of the collectin family of collagen-like lectins that function in host defense...
  11. Protein inhibitor of galectin-3 to limit fibrosis after myocardial infarction
    MATTHEW LAWRENCE SPRINGER; Fiscal Year: 2013
    ..Galectin- 3 is one of the galectin family of lectins that has homologous carbohydrate recognition domains and characteristic affinity for [unreadable]-galactosides...
  12. Synergy between TLR2 and IL-22R in airway epithelial cells during MRSA challenge
    Jeremy P McAleer; Fiscal Year: 2013
    ..infection, and also synergizes with IL-22R to mediate host defense through the induction of Reg3 antimicrobial lectins. This hypothesis will be tested with the following aims: Specific Aim 1: Determine the roles of TLR2 and IL-22R1 ..
  13. BIOLOGY OF TRABECULAR RESPONSE TO LASER TRABECULOPLASTY
    Ted S Acott; Fiscal Year: 2012
    ..Standard light and confocal microscopy using GAG stains, antibodies, binding proteins and lectins with enzymatic degradation will be used...
  14. Evaluation of the Effects of Chikungunya N-linked Glycosylation on Pathogenesis
    CHARLES EDWIN MCGEE; Fiscal Year: 2013
    ..from stimulation of innate immune activation via interactions between viral N-linked glycans and host C-type lectins. Experiments have been designed to identify specific interactions between a panel of CHIKV deficient for known ..
  15. Mechanisms of pneumococcal persistence during carriage.
    JEFFREY NEAL WEISER; Fiscal Year: 2013
    ..In specific aim#2, we will identify the macrophage receptor(s) (including scavenger receptors and C-type lectins) required for non-opsonic clearance during colonization...
  16. Synthetic Gypsogenin Saponins as Synergistic Vaccine Adjuvants
    ASHISH KUMAR PATHAK; Fiscal Year: 2013
    ..g. toll-like receptors (TLRs) and lectins, and ii) lipophilic chais, that may be designed to serve as ligands for innate immunity receptors...
  17. Protein Carbohydrate Interactions in Cell Communication
    James C Paulson; Fiscal Year: 2010
    ..The roles of protein-carbohydrate interactions in aspects of innate and acquired immunity, including C-type lectins, siglecs and the CD1 T-cell receptor in dendritic cells, macrophages, natural killer cells and other leukocytes, ..
  18. VESTIBULO-CEREBELLAR CIRCUITS
    CAREY DAVID BALABAN; Fiscal Year: 2013
    ..Hence, the experiments will shed new light of mechanisms accounting for the co-morbidity of vertigo/dizziness and migraine symptoms in patients. ..
  19. IMMUNOBIOLOGY OF SCHISTOSOME-MOLLUSC INTERACTIONS
    Timothy P Yoshino; Fiscal Year: 2013
    ..and molecular approach to evaluate the role of hemocyte pattern recognition receptors, emphasizing CHO-reactive lectins, in immune recognition of and reactivity to schistosome-specific CHOs...
  20. Lectin-Carbohydrate Interactions in the Host-Parasite System
    Xiao Qiang Yu; Fiscal Year: 2010
    ..The C-type lectins are a family of proteins with functions as PRRs in innate immunity...
  21. Development of novel immunotherapy for influenza virus infection
    Kazue Takahashi; Fiscal Year: 2011
    ..Preliminary studies demonstrate that these novel chimeric lectins bind mannan, the same carbohydrate that is expressed on influenza viruses and activate the lectin complement ..
  22. An Advanced Model to Analyze CD4T Cel Reactions to Antibody and BCR V Regions
    Lawrence J Wysocki; Fiscal Year: 2012
    ..may also activate CD4 T cells at sites of Ab deposition where APC gather Ab through Fc receptors or surface lectins. At present, we have only a rudimentary understanding of how the immune system regulates potentially dangerous ..
  23. Evolutionary lead optimization for immunotherapy of Marburg & Ebola viruses
    EMMETT VANCE SCHMIDT; Fiscal Year: 2010
    ..Furthermore, we will investigate the protective efficacy of these lectins in strains of genetically modified mice deficient in MBL, complement component 3, ficolin-A (murine form of L-..
  24. Functional Analysis of Galectin-1 Ligands in Melanoma Progression
    Charles J Dimitroff; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Cell surface carbohydrates and proteins (lectins) that bind them are conspicuously elevated in certain malignancies...
  25. GENETIC MODULATION OF BLOOD AND VASCULAR GLYCOSYLATION
    NISSI MARY VARKI; Fiscal Year: 2012
    ..Specific glycan-binding proteins differentially recognize these Sias, including CD33-related Siglecs (l-type lectins with cytosolic signaling motifs, on specific blood cell types) (Project 2)...
  26. Virulence Mechanisms in Candida Glabrata
    Carlos A Gomez; Fiscal Year: 2010
    ..studies: 1) The lab has previously shown, primarily using whole cell binding assays, that the Epa proteins are lectins, and has analyzed the glycan specificity for a subset of the Epa proteins...
  27. Preventing fucose-dependent binding of aspergillus and pseudomonas to lung mucin
    John V Fahy; Fiscal Year: 2012
    ..to mucins and mucin-covered cell surfaces, and we will inhibit the mucin cross-linking caused by these multimeric lectins. We have a multidisciplinary group that includes Drs Roy and Oscarson, two glycochemists with long experience in ..
  28. Mechanism of sepsis development in pulmonary bacterial infection
    Jyotika Sharma; Fiscal Year: 2013
    ..with a protective mutant show an upregulation and extracellular release of galectin-3 and galectin-9, two host lectins lacking secretion signal and exhibiting immune modulatory properties (characteristic of alarmins), only during ..
  29. Function of Ret-GFL signaling in sensory neurons
    Judith Golden; Fiscal Year: 2009
    ..This novel mouse will allow us to determine the role of the GFLs in nociceptor survival and function and to study mechanisms by which GFLs are effective in the treatment of chronic pain syndromes. ..
  30. Affinity Based Strategies to Fast Track Development of Colon Cancer Biomarkers
    Paul D Lampe; Fiscal Year: 2013
    ..incubated with plasma or tissue lysate for proteomic comparison or glycosylation (via incubation with different lectins)...
  31. Regulation of vascular leakage in age related macular degeneration
    MEREDITH GREGORY-KSANDER; Fiscal Year: 2013
    ....
  32. Microenvironment-Leukemia Communication Through Lectins
    NORA C HEISTERKAMP; Fiscal Year: 2013
    ....
  33. Composition, Structure, Dynamics and Function of C-Type Lectin Receptor Domains
    Nancy L Thompson; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): C-type lectins constitute a key class of pathogen receptors on dendritic cells (DCs), one of the main antigen presenting cell types in the immune system...
  34. ASSEMBLY AND TRANSFER OF N-LINKED OLIGOSACCHARIDES
    JAMES REID GILMORE; Fiscal Year: 2013
    ..hypoglycosylated proteins are delivered to the STT3B isoform of the OST by lumenal ER chaperones or ER-localized lectins. Inherited defects in the assembly pathway for the dolichol-linked oligosaccharide donor (Dol-PP-OS) for N-linked ..
  35. Glycobiology of Giardia
    John C Samuelson; Fiscal Year: 2013
    ..The second hypothesis is that the cyst wall of Giardia is composed of a small number of proteins, which are lectins that bind fibrils of the GalNAc homopolymer...
  36. High-specificity affinity reagents for N-glycosylation site mapping and glycomics
    Robert J Woods; Fiscal Year: 2013
    ..Lectenz(r) have several potential advantages over lectins and antibodies as glycomics reagents, including precise definition of specificity, ease of preparation in a ..
  37. Activation Defects in T Cells of Aged Mice
    Richard A Miller; Fiscal Year: 2011
    ..Aim 1 a will use a battery of specific lectins and glycosidases to determine which T cell surface glycoproteins contribute to diminished synapse formation, ..
  38. Analytical tools for the analysis of clustered O-glycans in clinical samples
    Matthew B Renfrow; Fiscal Year: 2013
    ..patients with IgAN;2) Define the range of clustered O-glycan structures that are recognized by five different lectins;and 3) Develop strategies for the quantitative assessment of individual protein and peptide clustered O-..
  39. Dendritic Cell-Associated C-Type Lectins
    Edward A Clark; Fiscal Year: 2013
    ....
  40. Harnessing Human DC Subsets for Improved Muscosal Vaccines
    Gerard Zurawski; Fiscal Year: 2013
    ..We have shown that antigens delivered to a single type of human DCs through different surface lectins induce distinct types of antigen-specific CD4+ T cell responses...
  41. Distinctive Glycan Fingerprints of Pancreatic Cancer for Plasma Detection
    David E Misek; Fiscal Year: 2013
    ..glycan structure as a function of cancer using the reactivity of tumor glycoproteins to fluorescently labeled lectins as a probe, as measured by differences in the intensity of the fractions arrayed from the individual samples...
  42. Defective Membrane Repair in Diabetes
    Paul L McNeil; Fiscal Year: 2012
    ..We have previously shown that binding of glycosylated cell surface proteins to exogenous lectins can potently inhibit membrane repair...
  43. Protein carbohydrate interactions in the pathophysiology of asthma exacerbations
    John V Fahy; Fiscal Year: 2013
    ..We hypothesize that mucin polymers are cross-linked by endogenous multimeric lectins specific for galactosyl residues in their O-linked glycans...
  44. Protein-glycan Interactions in the Vascular System
    Lijun Xia; Fiscal Year: 2013
    ..The data obtained will enhance our understanding of the functions of lectins and glycoconjugates during inflammation, thrombosis, and vascular development...
  45. Controlling Allergen-specific Th2-type Responses by Targeting DC Surface Lectins
    SangKon Oh; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Controlling allergen-specific Th2-type responses by targeting DC surface lectins (PI: Oh, SangKon) Increasing numbers of patients with allergic diseases demand safe and long-term effective ..
  46. Bacterial regulation of intestinal antimicrobial defense
    Lora V Hooper; Fiscal Year: 2013
    ..thus represents a previously unappreciated mechanism of mucosal defense and a new biological function for C-type lectins. Intestinal bacteria regulate RegIIIg transcription through direct activation of epithelial cell Toll-like ..
  47. Glycobiological Analysis of Plasmodium-Vector Host Interactions
    RHOEL DAVID RAMOS DINGLASAN; Fiscal Year: 2010
    ..chromatography followed by tandem mass spectrometry to identify glycoproteins that are recognized by specific lectins. Conversely, we anticipate that the process will be successful in identifying lectins that can bind to defined ..
  48. Functional Glycomics of Human Saliva
    Susan J Fisher; Fiscal Year: 2013
    ..Our approach includes capture by immobilized lectins and antibodies, which recognize specific aspects of oligosaccharide structure including linkage positions and ..
  49. Clinical and Molecular Phenotypes of Severe Asthma
    John V Fahy; Fiscal Year: 2013
    ..clinical and biological characteristics of these subgroups, and explore in ex vivo studies the role of multimeric lectins as cross-linkers of mucin polymers and potential targets of glycomimetic therapy...
  50. Novel Reagents For The Detection Of Low Abundance Cancer Specific Glycoforms
    MICHAEL JOHN CIOCCI; Fiscal Year: 2010
    ..The specific aims of this grant are focused on developing high affinity binding lectins (carbohydrate binding proteins) that recognize the glycsoylation changes that occur on serum biomarkers of HCC (..
  51. Probing Function of the C-type Lectin DC-SIGN with Glycomimetic Ligands
    Lynne Prost; Fiscal Year: 2011
    ..Compare ligand specificity of DC-SIGN to that of other lectins. Ligands found to bind with high affinity to DC-SIGN will be tested for binding to additional C-type lectins ..
  52. T CELL TARGETING FOR GVHD
    Bruce R Blazar; Fiscal Year: 2012
    ..T cell immunoglobulin, mucin-containing type I membrane glycoproteins (TIM family) present on T cells and S-type lectins (galectins) that recognize TIM carbohydrates present on immune system cells and on a wide range of tissue cells...
  53. Overcoming tumor cell resistance to apoptosis with a natural product, GCS-100
    Linda G Baum; Fiscal Year: 2010
    ..Galectin-3, a member of the galectin family of mammalian lectins, promotes cell survival, so that galectin-3 overexpression promotes tumor cell resistance to apoptosis and thus ..
  54. STRUCTURAL GLYCOMICS OF THE E. COLI HOST-PATHOGEN INTERFACE
    Bradley A Ford; Fiscal Year: 2012
    ..This process is most often mediated by carbohydrate-binding proteins (lectins) called adhesins that are assembled at the tips of hairlike extracellular appendages called pili...
  55. Galectin Modulation of Glutamate Receptors and Neuronal Function
    Geoffrey T Swanson; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The purpose of this project is to understand the role of animal-derived lectins in nervous system activity...
  56. Carbohydrate Receptors, Preterm Labor, and Periodontitis
    Wenge Ma; Fiscal Year: 2009
    ..are carried by mouse salivary and uterine mucins by evaluating their reactivity with a bank of antibodies and lectins that specifically react with these carbohydrate motifs...
  57. Pathogenesis of Fungal Keratitis
    Eric Pearlman; Fiscal Year: 2013
    ..Aim 1 will examine the role of c-type lectins in IL-6 and IL-23 production by macrophages and dendritic cells in response to these pathogens...
  58. Immune Responses to Schistosome Egg Antigens
    Donald A Harn; Fiscal Year: 2010
    ..Alternative activation of human DCs by soluble egg antigens occurs through three Ctype lectins: mannose receptor (MR), macrophage galactose receptor (MgI) and DC-SIGN...
  59. IMMUNOBIOLOGY OF SCHISTOSOME-MOLLUSC INTERACTIONS
    TIMOTHY YOSHINO; Fiscal Year: 2009
    ..hypothesis to be addressed is that parasite CHOs represent the recognition receptors, emphasizing CHO-reactive lectins, in immune recognition of and reactivity to expressed carbohydrates (CHO5) and hemocyte immune function...
  60. Synthesis of Complex Carbohydrates.
    Hien M Nguyen; Fiscal Year: 2013
    ..occurring oligosaccharides and glyco-conjugates, which play a crucial role as cell-surface receptor ligands for lectins, antibodies, and enzymes...
  61. SENSITIVE METHODS FOR GLYCOCONJUGATE ANALYSIS
    Milos Novotny; Fiscal Year: 2013
    ..glycoproteins from physiological fluids and tissues will be sought through the use of (a) surface-immobilized lectins;(b) surface-immobilized Protein L and selected antibodies;and (c) molecular size-based fractionation through ..
  62. Sensitive biomarker detection using organized nanoparticles in multiwell plates
    Mary J Wirth; Fiscal Year: 2010
    ..the sensitivity of ELISA assays in multi-well plates by at least an order of magnitude, and uniquely enable lectins to be used to probe glycosylation of biomarkers. This technology would enable earlier detection of cancer...
  63. Multiple Lectin Model of the Cyst Wall of Entamoeba
    John C Samuelson; Fiscal Year: 2010
    ..Similar 8-Cys chitin-binding domains are present in a novel family of Entamoeba lectins called Jessie...
  64. IMMUNE-LIKE PROTEINS OF INVERTEBRATES
    JOHN MARCHALONIS; Fiscal Year: 1993
    ..This proposal follows from a previous grant (GM 30672) on "animal lectins as recognition molecules", finding that certain carbohydrate-binding lectins of tunicate species could be ..
  65. CELL ADHESION AND CARBOHYDRATE LIGANDS
    ROGER LAINE; Fiscal Year: 1993
    ..Adhesion Molecule, ELAM-1), L-Selectin, and P-Selectin (GMP140) were a series of new calcium-binding lectins (C-lectins) which played a role in leukocyte adhesion to endothelium and extravasation...
  66. STRUCTURE OF THE RECEPTORS FOR ORAL BACTERIAL ADHESINS
    C Bush; Fiscal Year: 1991
    ..by streptococci and gram-positive rods which have been shown to coaggregate by the interaction of fimbrial lectins with polysaccharide receptors...
  67. EFFECTS OF PHORBOL ESTERS ON LYMPHOCYTE STIMULATION
    Andrea Mastro; Fiscal Year: 1991
    ..Unlike cell lines, lymphocytes remain in Go until stimulated. Mitogenic lectins along with macro-phages induce production of interleukin 1 (IL1), interleukin 2 (IL2), and their receptors which ..
  68. MOLECULAR BIOLOGY AND FUNCTIONS OF EOSINOPHIL PROTEINS
    Steven Ackerman; Fiscal Year: 2005
    ..lysophospholipase (LPLase), but we have now shown it to be a member of the galectin superfamily of animal lectins based on amino acid sequence, 3D protein structure, lack of LPLase activity and gene structure...
  69. CELL SURFACE LECTINS AND INTERCELLULAR ADHESION
    Steven Rosen; Fiscal Year: 2009
    ..As the molecular machinery of normal lymphocyte homing is elucidated, new targets for anti-inflammatory therapeutics are likely to emerge. ..
  70. Altered M. tuberculosis Mannosylation and the Macrophage
    Larry S Schlesinger; Fiscal Year: 2010
    ..These lipoglycans bind to C-type lectins expressed on macrophages and dendritic cells (DC)...
  71. INNOVATIVE INTRAVESICAL TREATMENT APPROACH FOR IC
    Veer Bhavanandan; Fiscal Year: 2001
    ..is to modify the sulfated polysaccharides with specific saccharide ligands, so that they will bind to endogenous lectins in the bladder...
  72. Dendritic cells, Mucosal Immunity and C-type Lectins
    Edward Clark; Fiscal Year: 2009
    ..The well-defined oral microflora is ideal for addressing this question. We have identified several new C-type lectins designated DCAL-1, DCAL-2 and DCAL-3...