Genomes and Genes
Summary: Proteins that contain an iron-porphyrin, or heme, prosthetic group resembling that of hemoglobin. (From Lehninger, Principles of Biochemistry, 1982, p480)
Publications303 found, 100 shown here
- Toll-like receptor 9 mediates innate immune activation by the malaria pigment hemozoinCevayir Coban
Exploratory Research for Advanced Technology ERATO, Japan Science and Technology Agency JST, Osaka, Japan
J Exp Med 201:19-25. 2005..These data suggest that TLR9-mediated, MyD88-dependent, and CQ-sensitive innate immune activation by HZ may play an important role in malaria parasite-host interactions...
- Fzf1p regulates an inducible response to nitrosative stress in Saccharomyces cerevisiaeAaron Sarver
Chemistry and Chemical Biology Graduate Program, University of California, San Francisco, San Francisco, CA 94143, USA
Mol Biol Cell 16:4781-91. 2005..Our results suggest that the presence of *NO or *NO derivatives activates Fzf1p leading to transcriptional induction of a discrete set of target genes that function to protect the cell from *NO-mediated stress...
- Pure Hemozoin is inflammatory in vivo and activates the NALP3 inflammasome via release of uric acidJason W Griffith
Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06511, USA
J Immunol 183:5208-20. 2009..These data suggest that uric acid is released during malaria infection and may serve to augment the initial host response to hemozoin via activation of the NALP3 inflammasome...
- Innate inflammatory response to the malarial pigment hemozoinMarina T Shio
Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada
Microbes Infect 12:889-99. 2010..This review will focus on HZ biosynthesis and synthetic preparation, but in particular on its effect on host's innate inflammatory responses...
- Involvement of inflammatory chemokines in survival of human monocytes fed with malarial pigmentGiuliana Giribaldi
Department of Genetics, Biology and Biochemistry, University of Torino Medical School, Turin, Italy
Infect Immun 78:4912-21. 2010....
- Adaptations against heme toxicity in blood-feeding arthropodsAurelio V Graca-Souza
Instituto de Bioquimica Medica, Universidade Federal do Rio de Janeiro, Centro de Ciencias da Saude, Av Brigadeiro Trompowsky, S N, Cidade Universitaria, Ilha do Fundao, 21941 690 Rio de Janeiro, RJ, Brazil
Insect Biochem Mol Biol 36:322-35. 2006..In spite of the existence of a great molecular diversity of protective mechanisms, different hematophagous organisms developed convergent solutions that may be physiologically equivalent...
- Identification and characterization of the hemophore-dependent heme acquisition system of Yersinia pestisM S Rossi
, 75724 Paris Cedex 15, France
Infect Immun 69:6707-17. 2001..This report constitutes the first attempt to investigate the contribution of the hemophore-dependent heme acquisition system in bacterial pathogenicity...
- Natural hemozoin stimulates syncytiotrophoblast to secrete chemokines and recruit peripheral blood mononuclear cellsN W Lucchi
Department of Infectious Diseases, University of Georgia, Athens, GA 30602, USA
Placenta 32:579-85. 2011....
- Malaria-parasitized erythrocytes and hemozoin nonenzymatically generate large amounts of hydroxy fatty acids that inhibit monocyte functionsEvelin Schwarzer
Institute of Biochemistry, Humboldt University Charite, Berlin, Germany
Blood 101:722-8. 2003....
- The role of neutral lipid nanospheres in Plasmodium falciparum haem crystallizationJohn M Pisciotta
Malaria Research Institute, W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, 615 N Wolfe Street, Baltimore, MD 21205, USA
Biochem J 402:197-204. 2007....
- Aligned hemozoin crystals in curved clusters in malarial red blood cells revealed by nanoprobe X-ray Fe fluorescence and diffractionSergey Kapishnikov
Department of Materials and Interfaces, The Weizmann Institute of Science, Rehovot 76100, Israel
Proc Natl Acad Sci U S A 109:11184-7. 2012..The diffraction results are verified by a study of the nucleation process using emerging tools of three-dimensional cellular microscopy, described in the companion paper...
- The IL-12p70/IL-10 interplay is differentially regulated by free heme and hemozoin in murine bone-marrow-derived macrophagesMathieu Cambos
Department of Biological Sciences, Universite du Quebec a Montreal, Montreal, Que, Canada
Int J Parasitol 40:1003-12. 2010..Our results suggest that oxidant stress induced by free heme may potentially contribute to sustained production of IL-10 and down-regulation of IL-12 responses in malaria...
- Transmembrane heme delivery systemsB S Goldman
Department of Biology, Washington University, One Brookings Drive, St Louis, MO 63130, USA
Proc Natl Acad Sci U S A 95:5003-8. 1998..We believe that a directed heme delivery pathway is vital for the synthesis of cytochromes c, whereby heme iron is protected from oxidation via ligation to histidinyl residues within the delivery proteins...
- Haem homeostasis is regulated by the conserved and concerted functions of HRG-1 proteinsAbbhirami Rajagopal
Department of Animal and Avian Sciences, University of Maryland, College Park, Maryland 20742, USA
Nature 453:1127-31. 2008..Thus, uncovering the mechanisms of haem transport in C. elegans may provide insights into human disorders of haem metabolism and reveal new drug targets for developing anthelminthics to combat worm infestations...
- Malaria hemozoin is immunologically inert but radically enhances innate responses by presenting malaria DNA to Toll-like receptor 9Peggy Parroche
Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, MA 01605, USA
Proc Natl Acad Sci U S A 104:1919-24. 2007..However, its activity depends on being bound to HZ, which we propose amplifies the biological responses to malaria DNA by targeting it to a TLR9(+) intracellular compartment...
- Haemozoin detection in infected erythrocytes for Plasmodium falciparum malaria diagnosis-prospects and limitationsMaria Rebelo
Instituto de Medicina Molecular, Faculdade de Medicina, Lisbon, Portugal
Acta Trop 123:58-61. 2012..Our results strongly suggest that, although it may be useful for monitoring maturation, detection of intra-erythrocytic Hz by flow cytometry will not provide an optimal method for diagnosis of P. falciparum malaria...
- A simple and inexpensive haemozoin-based colorimetric method to evaluate anti-malarial drug activityTran Thanh Men
Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo ku, Kyoto 606 8585, Japan
Malar J 11:272. 2012..This study describes an inexpensive procedure that is based on the advantage of a positive correlation between the haemozoin level of infected erythrocytes and parasite load...
- On the mechanisms involved in biological heme crystallizationRenata Stiebler
Instituto de Bioquimica Medica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil
J Bioenerg Biomembr 43:93-9. 2011....
- Haemozoin induces early cytokine-mediated lysozyme release from human monocytes through p38 MAPK- and NF-kappaB-dependent mechanismsManuela Polimeni
Dipartimento di Genetica, Biologia e Biochimica, Facolta di Medicina e Chirurgia, Universita degli Studi di Torino, Torino, Italy
PLoS ONE 7:e39497. 2012....
- Suppression of erythropoiesis in malarial anemia is associated with hemozoin in vitro and in vivoCliment Casals-Pascual
Nuffield Department of Clinical Laboratory Sciences and National Blood Service Oxford Centre, John Radcliffe Hospital, Oxford OX3 9BQ, United Kingdom
Blood 108:2569-77. 2006..Taken together, these observations provide compelling evidence for inhibition of erythropoiesis by Hz...
- The mechanism of direct heme transfer from the streptococcal cell surface protein Shp to HtsA of the HtsABC transporterTyler K Nygaard
Department of Veterinary Molecular Biology, Montana State University, Bozeman, 59718, USA
J Biol Chem 281:20761-71. 2006..These results support a direct, rapid, and affinity-driven mechanism of heme and hemin transfer from the cell surface receptor Shp to the ATP-binding cassette transporter system...
- The neutral lipid composition present in the digestive vacuole of Plasmodium falciparum concentrates heme and mediates β-hematin formation with an unusually low activation energyAnh N Hoang
Department of Chemistry, Vanderbilt University, Station B351822, Nashville, Tennessee 37235, United States
Biochemistry 49:10107-16. 2010....
- Malaria pigment paralyzes dendritic cellsBritta C Urban
Centre for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Clinical Medicine, University of Oxford, Churchill Hospital, Old Road, Oxford, OX3 7LJ, UK
J Biol 5:4. 2006..A prime suspect, the parasite-produced pigment hemozoin, has now been clearly shown to mediate immunosuppression by inhibiting dendritic cell activity...
- Suppression of adaptive immunity to heterologous antigens during Plasmodium infection through hemozoin-induced failure of dendritic cell functionOwain R Millington
Division of Immunology, Infection and Inflammation, University of Glasgow, Glasgow G11 6NT, UK
J Biol 5:5. 2006....
- Nitric oxide dioxygenase: an enzymic function for flavohemoglobinP R Gardner
Division of Critical Care Medicine, Children s Hospital Medical Center, and Department of Pediatrics, University of Cincinnati, 3333 Burnet Avenue, Cincinnati, OH 45229, USA
Proc Natl Acad Sci U S A 95:10378-83. 1998....
- The redox activity of hemoglobins: from physiologic functions to pathologic mechanismsBrandon J Reeder
Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex, England
Antioxid Redox Signal 13:1087-123. 2010....
- Modulation of cytokine profiles by malaria pigment--hemozoin: role of IL-10 in suppression of proliferative responses of mitogen stimulated human PBMCPrakash Deshpande
National Centre for Cell Science NCCS, NCCS campus, Ganeshkhind, Pune 411007, India
Cytokine 28:205-13. 2004..Kinetics of IL-10 secretion analyzed up to day 6 in MO/MQ cultures fed with Hz revealed that high levels of IL-10 were secreted during the first 48 h after ingestion and decreased drastically at later time points...
- Improved methods for magnetic purification of malaria parasites and haemozoinCharles C Kim
Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA 94158, USA
Malar J 9:17. 2010..As these methods are not necessarily optimized for parasite purification, the relationship between magnetic field strength and the quantity and quality of yield during parasite purification was explored...
- Natural haemozoin modulates matrix metalloproteinases and induces morphological changes in human microvascular endotheliumMauro Prato
Dipartimento di Genetica, Biologia e Biochimica, Universita di Torino, Torino, Italy
Cell Microbiol 13:1275-85. 2011..These findings might help understanding the mechanisms of blood brain barrier damage during CM...
- Evaluation of unbound free heme in plant cells by differential acetone extractionNino A Espinas
Graduate School of Science, The University of Tokyo, Tokyo, Japan
Plant Cell Physiol 53:1344-54. 2012..The present data demonstrate that the combination of single-step acetone extraction following a sensitive heme assay is the ideal method for determining total and free heme in plants...
- Inhibiting Plasmodium falciparum growth and heme detoxification pathway using heme-binding DNA aptamersJacquin C Niles
Department of Chemistry, University of California, Berkeley, CA 94720 3220, USA
Proc Natl Acad Sci U S A 106:13266-71. 2009..Altogether, these data demonstrate that aptamers can be versatile tools with applicability in functionally dissecting important P. falciparum-specific pathways both in vitro and in vivo...
- Differential regulation of beta-chemokines in children with Plasmodium falciparum malariaDaniel O Ochiel
Department of Infectious Diseases and Microbiology, University of Pittsburgh, 130 DeSoto Street, 603 Parran Hall, Pittsburgh, Pennsylvania 15261, USA
Infect Immun 73:4190-7. 2005..Altered patterns of circulating beta-chemokines result, at least in part, from Hz-induced changes in beta-chemokine gene expression in blood mononuclear cells...
- Bacterial heme sources: the role of heme, hemoprotein receptors and hemophoresC Wandersman
Unité des Membranes Bactériennes, Institut Pasteur CNRS URA 1300, Paris Cedex 15, 75724, France
Curr Opin Microbiol 3:215-20. 2000..Recent genetic, biochemical and crystallization studies have started to unravel the mechanism and molecular interactions between heme-carrier proteins and components of bacterial heme assimilation systems...
- Simple flow cytometric detection of haemozoin containing leukocytes and erythrocytes for research on diagnosis, immunology and drug sensitivity testingRosangela Frita
Instituto de Medicina Molecular, Faculdade de Medicina de Lisboa, Lisbon, Hospital Universitario de Santa Maria, Av, Prof, Egas Moniz, P 1649 028 Lisboa, Portugal
Malar J 10:74. 2011..These can range from the ex-vivo and in-vitro detection and functional analysis of Hz-containing leukocytes to the detection of parasitized Red-Blood-Cells (pRBCs) to assess antimalarial activity...
- HDP-a novel heme detoxification protein from the malaria parasiteDewal Jani
Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, Virginia, United States of America
PLoS Pathog 4:e1000053. 2008..As Hz formation is critical for survival, involvement of HDP in this process suggests that it could be a malaria drug target...
- A colorimetric high-throughput beta-hematin inhibition screening assay for use in the search for antimalarial compoundsKanyile K Ncokazi
Department of Chemistry, University of Cape Town, Private Bag, Rondebosch 7701, South Africa
Anal Biochem 338:306-19. 2005..The quantitative in vitro antimalarial activities of a set of 13 aminoquinolines and quinoline methanols were found to correlate significantly with beta-hematin inhibition values determined using the assay...
- Flavohaemoglobin HmpX from Erwinia chrysanthemi confers nitrosative stress tolerance and affects the plant hypersensitive reaction by intercepting nitric oxide produced by the hostMartine Boccara
Laboratoire de Pathologie Végétale, UMR 217 INRA INAP G Paris VI, 16 rue Claude Bernard, 75005 Paris, France
Plant J 43:226-37. 2005....
- Role of the NF-κB transcription pathway in the haemozoin- and 15-HETE-mediated activation of matrix metalloproteinase-9 in human adherent monocytesMauro Prato
Dipartimento di Genetica, Biologia e Biochimica, Universita di Torino, Via Santena 5 bis, 10126 Torino, Italy
Cell Microbiol 12:1780-91. 2010....
- Interference with hemozoin formation represents an important mechanism of schistosomicidal action of antimalarial quinoline methanolsJuliana B R Corrêa Soares
Laboratório de Bioquímica Redox, Programa de Biologia Molecular e Biotecnologia, Instituto de Bioquimica Medica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
PLoS Negl Trop Dis 3:e477. 2009....
- Synthetic Plasmodium-like hemozoin activates the immune response: a morphology - function studyMaritza Jaramillo
The Research Institute of the McGill University Health Centre, Centre for the Study of Host Resistance, Departments of Medicine, Microbiology and Immunology, McGill University, Montreal, Canada
PLoS ONE 4:e6957. 2009..Periodic quality control experiments and unification of the method of HZ synthesis are key steps to unravel the role of HZ in malaria immunopathology...
- Naturally acquired hemozoin by monocytes promotes suppression of RANTES in children with malarial anemia through an IL-10-dependent mechanismTom Were
University of New Mexico Kenya Medical Research Institute, Laboratories of Parasitic and Viral Diseases, Centre for Global Health Research, Kisumu, Kenya
Microbes Infect 11:811-9. 2009..028) in PBMC from children with naturally acquired Hz. Results here demonstrate that monocytic acquisition of Hz suppresses RANTES production in children with MA through an IL-10-dependent mechanism...
- Species specificity, surface exposure, protein expression, immunogenicity, and participation in biofilm formation of Porphyromonas gingivalis HmuYTeresa Olczak
Laboratory of Biochemistry, Faculty of Biotechnology, University of Wroclaw, Tamka 2, 50 137 Wroclaw, Poland
BMC Microbiol 10:134. 2010..The aim of this study was to examine the species specificity, surface exposure, protein expression, immunogenicity, and participation in biofilm formation of the P. gingivalis heme-binding protein HmuY...
- Isolation and characterization of an extracellular haem-binding protein from Pseudomonas aeruginosa that shares function and sequence similarities with the Serratia marcescens HasA haemophoreS Letoffe
Unité de Physiologie Cellulaire Institut Pasteur CNRS URA 1300, Paris, France
Mol Microbiol 28:1223-34. 1998..HasAp, like HasA, lacks a signal peptide and is secreted by an ABC transporter. These findings show that haemophore-dependent haem acquisition is not unique to S. marcescens...
- Heme protein assembliesCharles J Reedy
Department of Chemistry, Columbia University, 3000 Broadway, MC 3121, New York, New York 10027, USA
Chem Rev 104:617-49. 2004
- Diversity and conservation of interactions for binding heme in b-type heme proteinsSabine Schneider
School of Pharmacy and Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, England, UK
Nat Prod Rep 24:621-30. 2007
- Mitochondrial cytochrome oxidase produces nitric oxide under hypoxic conditions: implications for oxygen sensing and hypoxic signaling in eukaryotesPablo R Castello
The Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309, USA
Cell Metab 3:277-87. 2006....
- Magnetic field enriched surface enhanced resonance Raman spectroscopy for early malaria diagnosisClement Yuen
Nanyang Technological University, Division of Bioengineering, School of Chemical and Biomedical Engineering, Singapore 637457
J Biomed Opt 17:017005. 2012....
- A magneto-optic route toward the in vivo diagnosis of malaria: preliminary results and preclinical trial dataDave M Newman
School of Engineering, Computing and Mathematics, University of Exeter, Exeter EX4 4QF, United Kingdom
Biophys J 95:994-1000. 2008....
- Identification and characterization of HtsA, a second heme-binding protein made by Streptococcus pyogenesBenfang Lei
Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
Infect Immun 71:5962-9. 2003..Our results suggest that HtsABC is an ABC transporter that may participate in heme acquisition in GAS...
- Heme uptake across the outer membrane as revealed by crystal structures of the receptor-hemophore complexStefanie Krieg
Fachbereich Biologie, Universitat Konstanz, 78457 Konstanz, Germany
Proc Natl Acad Sci U S A 106:1045-50. 2009..Subsequently, steric displacement of heme by a receptor residue ruptures the other axial coordination, leading to heme transfer into the receptor...
- The diversity of globin-coupled sensorsTracey Allen K Freitas
Department of Microbiology, Snyder Hall 207, 2538 The Mall, University of Hawaii, Honolulu, HI 96822, USA
FEBS Lett 552:99-104. 2003..The GCS superfamily is composed of two major subfamilies: the aerotactic and gene regulators. We postulate the existence of protoglobin in Archaea as the predecessor to the chimeric GCS...
- Increase on the initial soluble heme levels in acidic conditions is an important mechanism for spontaneous heme crystallization in vitroRenata Stiebler
Laboratório de Bioquímica Redox, Programa de Biologia Molecular e Biotecnologia, Instituto de Bioquimica Medica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
PLoS ONE 5:e12694. 2010..Here, we aimed to investigate the role of medium polarity on spontaneous βH formation in vitro...
- Computational investigation on the photoacoustics of malaria infected red blood cellsRatan K Saha
Ratan K Saha Applied Material Science Division, Saha Institute of Nuclear Physics, Kolkata, West Bengal, India
PLoS ONE 7:e51774. 2012..The presence of hemozoin inside the cells was examined and an excellent estimation was made. The simulation results suggest that intraerythrocytic stages of malarial parasite may be assessed using the PA technique...
- One ring to rule them all: trafficking of heme and heme synthesis intermediates in the metazoansIqbal Hamza
Department of Animal and Avian Sciences, University of Maryland, College Park, MD 20742, USA
Biochim Biophys Acta 1823:1617-32. 2012..Additionally, older, well established data are reexamined in light of more recent studies on cellular organization and data available from newer model organisms. This article is part of a Special Issue entitled: Cell Biology of Metals...
- Platelet accumulation in brain microvessels in fatal pediatric cerebral malariaGeorges E Grau
Experimental Parasitology Unit, IFR48, Universite de la Mediterranee, Marseille, France
J Infect Dis 187:461-6. 2003..Platelet accumulation occurs in the microvasculature of patients with CM and may play a role in the pathogenesis of the disease...
- Transcriptional regulation of the heme binding protein gene family of Bartonella quintana is accomplished by a novel promoter element and iron response regulatorJames M Battisti
Division of Biological Sciences, The University of Montana, Missoula, MT 59812, USA
Infect Immun 75:4373-85. 2007....
- Molecular characterization of the hemin uptake locus (hmu) from Yersinia pestis and analysis of hmu mutants for hemin and hemoprotein utilizationJ M Thompson
Department of Microbiology and Immunology, University of Kentucky, Lexington, Kentucky 40536 0084, USA
Infect Immun 67:3879-92. 1999..Growth studies showed that a DeltahmuP'RSTUV mutant could grow in iron-depleted medium containing high concentrations of hemoglobin, suggesting that an Hmu-independent, lower-affinity hemoglobin uptake system may exist...
- BhuR, a virulence-associated outer membrane protein of Bordetella avium, is required for the acquisition of iron from heme and hemoproteinsErin R Murphy
The Witebsky Center for Microbial Pathogenesis and Immunology, School of Medicine and Biomedical Sciences, University at Buffalo, State University of New York, Buffalo, New York 14214, USA
Infect Immun 70:5390-403. 2002..avium to demonstrate that the bacterium transports heme into the cytoplasm in a BhuR-dependent manner. The role of BhuR in virulence was established in turkey poults by use of a competitive-infection model...
- A novel way to grow hemozoin-like crystals in vitro and its use to screen for hemozoin inhibiting antimalarial compoundsVincent Thomas
STERIS SA R and D, Fontenay aux Roses, France
PLoS ONE 7:e41006. 2012..In this report we describe new ways to form hemozoin-like crystals that were incidentally discovered during research in the field of prion inactivation...
- Macrophage inflammatory protein-1alpha mediates matrix metalloproteinase-9 enhancement in human adherent monocytes fed with malarial pigmentGiuliana Giribaldi
Dipartimento di Genetica, Biologia e Biochimica, Facolta di Medicina e Chirurgia, Universita degli Studi di Torino, Italy
Asian Pac J Trop Med 4:925-30. 2011....
- Sensitive detection of malaria infection by third harmonic generation imagingJonathan M Belisle
Biophys J 94:L26-8. 2008..The immense nonlinear response of the intrinsic parasitic by-product pigments suggests that automated optical detection by THG could be used for sensitive and rapid screening of parasite infection in blood samples...
- Molecular characterization of a newly identified heme-binding protein induced during differentiation of urine erythroleukemia cellsS Taketani
Department of Hygiene, Kansai Medical University, Moriguchi, Osaka 570 8506, Japan
J Biol Chem 273:31388-94. 1998..Thus, this newly identified heme-binding protein, p22 HBP, may be involved in heme utilization for hemoprotein synthesis and even be coupled to hemoglobin synthesis during erythroid differentiation...
- Ultrafast dynamics of diatomic ligand binding to nitrophorin 4Abdelkrim Benabbas
Department of Physics and Center for Interdisciplinary Research on Complex Systems, Northeastern University, Boston, Massachusetts 02115, USA
J Am Chem Soc 132:2811-20. 2010..et al. Proc. Natl. Acad. Sci. U.S.A. 2007, 104, 14682)...
- Growth of Plasmodium falciparum induces stage-dependent haemichrome formation, oxidative aggregation of band 3, membrane deposition of complement and antibodies, and phagocytosis of parasitized erythrocytesG Giribaldi
Department of Genetics, Biology and Biochemistry, University of Torino Medical School, Torino, Italy
Br J Haematol 113:492-9. 2001....
- ABC transporter-mediated release of a haem chaperone allows cytochrome c biogenesisRobert E Feissner
Washington University, Department of Biology Campus Box 1137, 1 Brookings Drive, St Louis, MO 63130, USA
Mol Microbiol 61:219-31. 2006..We propose that the CcmA2B1C1 complex represents a new subgroup within the ABC transporter superfamily that functions to release a chaperone...
- The Janus nature of hemeThomas L Poulos
Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92697 3900, USA
Nat Prod Rep 24:504-10. 2007
- Hemozoin and the human monocyte--a brief review of their interactionsE Schwarzer
Department of Genetics, Biology and Biochemistry, University of Torino, Via Santena 5 bis, 10126 Torino, Italy
Parassitologia 50:143-5. 2008..Several HZ effects were reproduced by supplementation with plausible concentrations of HETE or HNE, the first most likely via interaction with PPAR-receptors, the second via adduct or crosslinks formation with critical targets...
- Trioxaquines and heme-artemisinin adducts inhibit the in vitro formation of hemozoin better than chloroquineChristophe Loup
Laboratoire de Chimie de Coordination, CNRS, 205 route de Narbonne, 31000, Toulouse Cedex 4, France
Antimicrob Agents Chemother 51:3768-70. 2007..Trioxaquines, potential antimalarial agents, and heme-artemisinin adducts, resulting from the alkylation of heme by artemisinin, were evaluated as inhibitors of beta-hematin formation in 10 M acetate medium at pH 5...
- The novel extracellular Streptomyces reticuli haem-binding protein HbpS influences the production of the catalase-peroxidase CpeBDarío Ortiz de Orué Lucana
Universitat Osnabruck, FB Biologie Chemie, Barbarastrasse 11, D 49069 Osnabruck, Germany
Microbiology 150:2575-85. 2004..vi) HbpS shared varying degrees of amino acid identities with bacterial proteins of so far unknown function. This report contributes to the elucidation of the biological function of these proteins...
- Yeast flavohemoglobin protects against nitrosative stress and controls ferric reductase activityAnna Lewinska
Department of Biochemistry and Cell Biology, University of Rzeszow, Rzeszow, Poland
Redox Rep 11:231-9. 2006..Deletion of the YHB1 gene was found to augment the reduction of Fe(3+) by yeast cells which suggests that flavohemoglobin participates in regulation of the activity of plasma membrane ferric reductase(s)...
- Alpha-glucosidase promotes hemozoin formation in a blood-sucking bug: an evolutionary historyFlávia Borges Mury
Laboratório de Química e Função de Proteínas e Peptídeos, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Rio de Janeiro, Brazil
PLoS ONE 4:e6966. 2009..In Rhodnius prolixus, hemoglobin-derived heme is detoxified by biomineralization, forming hemozoin (Hz). Recently, the involvement of the R. prolixus perimicrovillar membranes in Hz formation was demonstrated...
- Unique structure and stability of HmuY, a novel heme-binding protein of Porphyromonas gingivalisHalina Wojtowicz
Laboratory of Biochemistry, Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland
PLoS Pathog 5:e1000419. 2009..It is also stable against thermal and chemical denaturation. In conclusion, these studies reveal novel molecular properties of HmuY that are consistent with its role as a putative virulence factor during bacterial infection...
- Enhanced phagocytosis of ring-parasitized mutant erythrocytes: a common mechanism that may explain protection against falciparum malaria in sickle trait and beta-thalassemia traitKodjo Ayi
Dipartimento di Genetica, Biologia e Biochimica, Universita di Torino, Italy
Blood 104:3364-71. 2004....
- Artemisinin antimalarials do not inhibit hemozoin formationRichard K Haynes
Antimicrob Agents Chemother 47:1175. 2003
- The hmu locus of Yersinia pestis is essential for utilization of free haemin and haem--protein complexes as iron sourcesJ M Hornung
Department of Microbiology and Immunology, University of Kentucky, Lexington 40536 0084, USA
Mol Microbiol 20:725-39. 1996....
- A bacterial cytochrome c heme lyase. CcmF forms a complex with the heme chaperone CcmE and CcmH but not with apocytochrome cQun Ren
Institut fur Mikrobiologie, Eidgenossische Technische Hochschule, Schmelzbergstrasse 7, CH 8092 Zurich, Switzerland
J Biol Chem 277:7657-63. 2002..We propose that CcmFH forms a bacterial heme lyase complex for the transfer of heme from CcmE to apocytochrome c...
- A simple and rapid colorimetric method to measure hemozoin crystal growth in vitroNguyen Tien Huy
Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo ku, Kyoto 606 8585, Japan
Anal Biochem 354:305-7. 2006
- Identification of amino acid residues involved in heme binding and hemoprotein utilization in the Porphyromonas gingivalis heme receptor HmuRXinyan Liu
Department of Medicine, Section of Infectious Diseases, Boston University School of Medicine, 650 Albany St, Boston, MA 02118, USA
Infect Immun 74:1222-32. 2006..Taken together, these results support a role for H95, H434, and the NPDL motif of the P. gingivalis HmuR protein in heme binding and utilization of serum hemoproteins and the HmuR YRAP motif in serum hemoprotein utilization...
- Heme environment in HmuY, the heme-binding protein of Porphyromonas gingivalisHalina Wojtowicz
Faculty of Biotechnology, Laboratory of Biochemistry, University of Wrocław, Wrocław, Poland
Biochem Biophys Res Commun 383:178-82. 2009..Analysis of heme binding to several single and double HmuY mutants with the methionine, histidine, cysteine, or tyrosine residues replaced by an alanine residue identified histidines 134 and 166 as potential heme ligands...
- Extracellular lipid droplets promote hemozoin crystallization in the gut of the blood fluke Schistosoma mansoniJuliana B R Corrêa Soares
Instituto de Bioquimica Medica, Programa de Biologia Molecular e Biotecnologia, Universidade Federal do Rio de Janeiro, Cidade Universitaria, Rio de Janeiro, RJ, Brazil
FEBS Lett 581:1742-50. 2007..mansoni. Thus, these data demonstrate that LDs present in the gut lumen of S. mansoni support Hz formation possibly by allowing association of heme to the lipid-water interface of these structures...
- Crystal structure and biochemical features of EfeB/YcdB from Escherichia coli O157: ASP235 plays divergent roles in different enzyme-catalyzed processesXiuhua Liu
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China
J Biol Chem 286:14922-31. 2011..In addition, dramatically enhanced fluorescence excitation and emission of EfeB-PPIX was observed, implying this protein may be used as a red color fluorescence marker...
- Simple colorimetric inhibition assay of heme crystallization for high-throughput screening of antimalarial compoundsNguyen Tien Huy
Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo ku, Kyoto 606 8585, Japan
Antimicrob Agents Chemother 51:350-3. 2007....
- Hematological predictors of increased severe anemia in Kenyan children coinfected with Plasmodium falciparum and HIV-1Gregory C Davenport
Department of Infectious Diseases and Microbiology, University of Pittsburgh, Pittsburgh, PA, USA
Am J Hematol 85:227-33. 2010..Thus, malaria/HIV-1 coinfection is characterized by more profound anemia and increased mortality, with acquisition of monocytic pigment having the most detrimental impact on Hb levels...
- Inhibitory effect of ursolic acid derivatives on hydrogen peroxide- and glutathione-mediated degradation of hemin: a possible additional mechanism of action for antimalarial activityCatherine Mullié
Laboratoire des Glucides UMR CNRS 6219, Faculte de Pharmacie, rue des Louvels, Amienx Cedex 1, France
Exp Parasitol 125:202-7. 2010..For GSH degradation pathway, grafting of UA structure with a piperazine structure gave the best inhibition, pleading for the implication of this latter moiety in the inhibitory process...
- Characterization of cytosolic tetrapyrrole-binding proteins in Arabidopsis thalianaShigekazu Takahashi
Department of General Systems Studies, Graduate School of Arts and Sciences, The University of Tokyo, Komaba 3 8 1, Tokyo, 153 8902, Japan
Photochem Photobiol Sci 7:1216-24. 2008..These results suggest that both cHBP1 and cHBP2 have properties suitable for tetrapyrrole carrier proteins and function in distinct organs in plant cells...
- Plasmodium berghei: in vitro and in vivo activity of dequaliniumJuan Ricardo Rodrigues
Unidad de Bioquimica, Facultad de Farmacia, Universidad Central de Venezuela, Apartado 47206, Los Chaguaramos, Caracas 1051 A, Venezuela
Exp Parasitol 115:19-24. 2007..These results confirm that dequalinium is a promising lead for antimalarial drug development...
- Inhibition assay of beta-hematin formation initiated by lecithin for screening new antimalarial drugsDai Thi Xuan Trang
Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo ku, Kyoto 606 8585, Japan
Anal Biochem 349:292-6. 2006..We also indicated that dimethyl sulfoxide is an ideal solvent for preparation of heme stock solution, which is stable and can be used for 1 month...
- Loss of ATP hydrolysis activity by CcmAB results in loss of c-type cytochrome synthesis and incomplete processing of CcmEOlaf Christensen
Institut fur Mikrobiologie, Eidgenossische Technische Hochschule, Zurich, Switzerland
FEBS J 274:2322-32. 2007..coli with a ccmA deletion did not restore cytochrome c production. Our results suggest a role for CcmAB in the handling of heme by CcmE, which is chemically complex and involves an unusual histidine-heme covalent bond...
- Hemin binding protein C is found in outer membrane vesicles and protects Bartonella henselae against toxic concentrations of heminJulie A Roden
Microbial Pathogenesis and Host Defense Program, Department of Medicine, University of California at San Francisco, San Francisco, California, USA
Infect Immun 80:929-42. 2012..henselae to infect the cat host. Modulation of hbpC expression appears to be a strategy employed by B. henselae to survive in the arthropod vector and the mammalian host...
- Ellagitannins of the fruit rind of pomegranate (Punica granatum) antagonize in vitro the host inflammatory response mechanisms involved in the onset of malariaMario dell'Agli
Dipartimento di Scienze Farmacologiche, Universita degli Studi di Milano, Via Balzaretti, 9 20133 Milano, Italy
Malar J 9:208. 2010..The aim of the present study was to explore whether the anti-malarial effect of OMARIA could also be sustained via other mechanisms among those associated to the host immune response...
- The HP-1 maquette: from an apoprotein structure to a structured hemoprotein designed to promote redox-coupled proton exchangeSteve S Huang
The Johnson Research Foundation and Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104, USA
Proc Natl Acad Sci U S A 101:5536-41. 2004..Oxidation-reduction of both hemes displays an unusually low midpoint potential (-248 mV vs. normal hydrogen electrode at pH 9.0), which is strongly coupled to proton binding, as designed...
- A pivotal heme-transfer reaction intermediate in cytochrome c biogenesisDespoina A I Mavridou
Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom
J Biol Chem 287:2342-52. 2012..We propose the complex is a trapped catalytic intermediate in the cytochrome c biogenesis process, at the point of heme transfer from CcmE to the cytochrome, the key step in the maturation pathway...
- Physical interaction of CcmC with heme and the heme chaperone CcmE during cytochrome c maturationQ Ren
Institut fur Mikrobiologie, Eidgenossische Technische Hochschule, Schmelzbergstrasse 7, CH 8092 Zurich, Switzerland
J Biol Chem 276:32591-6. 2001..We propose that CcmC, CcmE, and heme interact directly with each other, establishing a periplasmic heme delivery pathway for cytochrome c maturation...
- Haem-polypeptide interactions during cytochrome c maturationL Thony-Meyer
Institute of Microbiology, ETH Zurich, Schmelzbergstrasse 7, CH 8092, Zurich, Switzerland
Biochim Biophys Acta 1459:316-24. 2000..CcmC is required for binding of haem to CcmE, and CcmF for releasing it from CcmE and transferring it onto the apocytochrome. The mechanism by which haem crosses the cytoplasmic membrane is currently unknown...
- Bond or cage effect: how nitrophorins transport and release nitric oxideMarcelo A Marti
Departamento de Quimica Inorganica, Analitica, y Quimica Fisica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, INQUIMAE CONICET, Ciudad Universitaria, Pab 2, C1428EHA, Buenos Aires, Argentina
J Am Chem Soc 130:1611-8. 2008..In contrast to most heme proteins that control ligand affinity by modulating the bond strength to the iron, NP4 has evolved a cage mechanism that traps the NO at low pH and releases it upon cage opening when the pH rises...
- In situ localization and structural analysis of the malaria pigment hemozoinTorsten Frosch
Institut fur Physikalische Chemie, Friedrich Schiller Universitat Jena, Helmholtzweg 4, D 07743 Jena, Germany
J Phys Chem B 111:11047-56. 2007....
- Haemozoin: from melatonin pigment to drug target, diagnostic tool, and immune modulatorThomas Hanscheid
Institute of Molecular Medicine, Lisbon Medical College, Lisbon, Portugal
Lancet Infect Dis 7:675-85. 2007..Finally, haemozoin has a substantial effect on the immune system. Further research is needed to clarify these aspects, many of which are important in clinical practice...
- Multi-frequency high-field EPR study of iron centers in malarial pigmentsAndrzej Sienkiewicz
Institute of Physics, Polish Academy of Sciences, Al Lotnikow 32 46, 02 668 Warsaw, Poland
J Am Chem Soc 128:4534-5. 2006..85(1) cm-1, E = 0, g perpendicular = 1.95(1), g parallel = 2.00(1). These results point to the existence of largely axial symmetry of the iron environment in the bulk phase of hemozoin and beta-hematin...
- Abnormal display of PfEMP-1 on erythrocytes carrying haemoglobin C may protect against malariaRick M Fairhurst
Laboratory of Malaria and Vector Research, Research Technologies Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
Nature 435:1117-21. 2005..Haemoglobin C might protect against malaria by reducing PfEMP-1-mediated adherence of parasitized erythrocytes, thereby mitigating the effects of their sequestration in the microvasculature...
- Heme proteins--diversity in structural characteristics, function, and foldingLorna J Smith
Department of Chemistry, University of Oxford, Inorganic Chemistry Laboratory, Oxford OX1 3QR, United Kingdom
Proteins 78:2349-68. 2010..The principles identified here provide a framework for the design of de novo proteins that will exhibit tight heme ligand binding and for the identification of the function of structural genomic target proteins with heme ligands...
- Differences in anti-malarial activity of 4-aminoalcohol quinoline enantiomers and investigation of the presumed underlying mechanism of actionCatherine Mullié
Laboratoire des Glucides, UMR CNRS 6219, UFR de Pharmacie, 1 rue des Louvels, 80037 Amiens Cedex 1, France
Malar J 11:65. 2012..Their in vitro activity on Plasmodium falciparum W2 and 3D7 strains is reported here along with their inhibition of β-haematin formation and peroxidative degradation of haemin, two possible mechanisms of action of anti-malarial drugs...
- Nitric Oxide Signaling with Heme ProteinsWilliam R Montfort; Fiscal Year: 2012..Our results are expected to uncover new approaches for drug discovery targeted to cardiovascular disease, cancer metastasis and related failures in human health. ..
- Biosynthetic Protein Models of Heme-copper Oxidases and Nitric Oxide ReductasesYi Lu; Fiscal Year: 2013..Therefore the work will make important contributions to healthcare, as it will provide a molecular basis for understanding two enzymes important to human health. ..
- RESEARCH CENTER FOR PHARMACOLOGY AND DRUG TOXICOLOGYL Jackson Roberts; Fiscal Year: 2010..It is anticipated that the projects proposed will significantly advance our understanding of the pharmacology and biology of eicosanoids and related compounds. ..
- Oxygen Sensors and P450 Monooxygenases in Mycobacertium tuberculosisPaul R Ortiz de Montellano; Fiscal Year: 2013..tuberculosis. ..
- NITRIC OXIDE INTERACTION WITH INSECT NITROPHORINSFRANCES ANN WALKER; Fiscal Year: 2012....
- New mechanism and regulation of intracellular heme delivery in mammalsDennis J Stuehr; Fiscal Year: 2013..Our findings will touch many aspects of human health and disease, including blood formation, drug response, anemia, and inflammation. ..
- Myoglobin as a Nitrite Reductase that Regulates Hypoxic Cardiac NO SignalingSruti Shiva; Fiscal Year: 2013..This project will investigate how nitrite and myoglobin work together so that nitrite can be used to protect the heart during a heart attack. ..
- Molecular Probes of the Mechanisms of Cytocrhome P450JOHN TAYLOR GROVES; Fiscal Year: 2013..abstract_text> ..
- HEME PROTEIN REDUCTASESStephen G Sligar; Fiscal Year: 2012..abstract_text> ..
- Soluble guanylyl cyclase modulation by endogenous elements and exogenous factorsEmil Martin; Fiscal Year: 2012..Although there are many examples of hemeproteins inhibited by interaction with NO, sGC is the only hemeproteins which is activated by NO binding...
- Elucidating Protein Folding MechanismsJay R Winkler; Fiscal Year: 2010..A clear understanding of the dynamics of polypeptide conformational motions is the first step on the pathway to new molecules that can help proteins avoid or escape from the incorrect structures that lead to disease. ..
- Protein, Enzyme, and Biological Water Dynamics: 2D Vibrational Echo SpectroscopyMichael D Fayer; Fiscal Year: 2011..The methodology builds on previous successful applications and developments of state-of-the-art ultrafast infrared laser experiments. ..
- Femtosecond Coherence Spectroscopy &Ultrafast Kinetic Study of Heme ProteinsPaul M Champion; Fiscal Year: 2012..Such non-equilibrium processes will be studied to learn if they allow heme proteins to enhance discrimination between different classes of diatomic ligands. ..
- Diversity in Heme Protein Structure and FunctionTHOMAS LEWIS POULOS; Fiscal Year: 2013..A new crystal structure of a peroxidase-cytochrome c complex has been solved which has provided a new system for probing inter-protein electron transfer. ..
- Homocysteine &Heme: A Role in Thrombosis?Jan P Kraus; Fiscal Year: 2012..abstract_text> ..
- HTS for Cytochrome C Synthesis PathwaysRobert G Kranz; Fiscal Year: 2011..Thus, the HTS will also discover new inhibitors of heme biosynthesis, and the PIs group has developed the tools to quickly determine which enzyme (of nine) is inhibited in heme synthesis. ..
- Development of photothermal microscopy for biomedical applicationsJEROME C MERTZ; Fiscal Year: 2012..e. non-fluorescent) proteins or molecules in tissue. This technology will be useful for in- vivo imaging research applications and rapid tissue diagnosis in the clinic. ..
- Cytochrome c biogenesisRobert G Kranz; Fiscal Year: 2013..Results here will unravel the mechanisms underlying heme transport, heme red-ox control, and heme attachment to all prokaryotic and plant c-type cytochromes. ..
- Conformational Properties of Cytochrome c in ApoptosisEKATERINA PLETNEVA; Fiscal Year: 2013....
- Design of Heme Protein-Based Blood SubstitutesJohn S Olson; Fiscal Year: 2010....
- Raman Studies of Mammalian Cytochromes P450JAMES ROBERT KINCAID; Fiscal Year: 2013..The resonance Raman technique being used in this work is one of the most powerful methods to acquire this critical information. ..
- Oxidation in Inflammation and Cardiovascular DiseaseStanley L Hazen; Fiscal Year: 2013..It may also lead to important insights for atherosclerosis risk assessment, diagnosis and therapy. ..
- 2012 Oxygen Radicals Gordon Research ConferenceNeil Hogg; Fiscal Year: 2012..The conference brings together the leading scientists in the field to share and discuss their current un-published research findings which will stimulate new endeavors and promote novel ways to improve human health. ..
- METABOLISM AND NUTRITION OF IRON AND OTHER TRACE METALSPAUL SALTMAN; Fiscal Year: 1993..There is a remarkable coherence among the three research goals in that site-specific binding, oxidation-reduction and chelation play significant roles in cellular metabolism of trace elements...
- MPO and NO signaling in neointima formationChunxiang Zhang; Fiscal Year: 2010..The current proposal will identify a novel mediator, leukocyte-derived MPO, in neointima formation and its molecular mechanism(s). Blocking the MPO pathway might be a new therapeutic approach to restenosis after balloon angioplasty. ..
- Structural Biology of Gaseous Messenger SignalingSudha Veeraraghavan; Fiscal Year: 2011..This proposal is aimed at understanding how nitric oxide works in healthy and disease states. ..
- HNO Interactions with Metalloproteins: Structures and MechanismsYong Zhang; Fiscal Year: 2013..Results will provide useful structural and mechanistic results of HNO interactions with metalloproteins and models to facilitate studies of health, diseases, and therapeutic treatments involving HNO. ..
- Electronic, Structural, and Kinetic Characterizations of Cytochrome P450 CompoundMichael T Green; Fiscal Year: 2013..Proposed experiments thus provide access to the magnitude of D(O-H), allowing insight into how P450s leverage redox potential and ferryl basicity to activate hydrocarbons. ..
- MECHANISMS INVOLVED IN FLAVIN-LINKED OXYGEN METABOLISMAl Claiborne; Fiscal Year: 2005..for the biochemical diversity in oxygen metabolism, due to their lack of respiratory cytochromes and other hemeproteins. Instead these organisms rely on networks of unusual flavin-linked respiratory enzymes, and this application is ..
- Heme Oxygenase and Spinal Cord InjuryLinda Noble; Fiscal Year: 2004..abstract_text> ..
- HEME PROTEINS, MICROSPHERES AND SYNTHETIC ANALOGSKENNETH SUSLICK; Fiscal Year: 2004....
- STRUCTURE AND DYNAMICS OF HEME PROTEIN ACTIVE SITESJAMES SATTERLEE; Fiscal Year: 2005..We shall proceed using modern protein engineering methods combined with x-ray crystallography, kinetics, photothermal methods, equillibrium dynamics methods and NMR spectroscopy. ..
- ELECTROSTATICS AND DYNAMICS IN MYOGLOBIN MUTANTSSTEVEN BOXER; Fiscal Year: 2003..This will permit a direct comparison of experimental data with predictions from molecular dynamics simulations. ..
- OXYGEN SENSING AND SIGNAL TRANSDUCTIONH Bunn; Fiscal Year: 2003..abstract_text> ..
- IRON PORPHYRIN ACTIVATION OF DIOXYGEN/SMALL MOLECULESALAN BALCH; Fiscal Year: 2004....
- TIME-RESOLVED MAGNETIC CIRCULAR DICHROISMDavid Kliger; Fiscal Year: 2002..abstract_text> ..
- Fast Kinetic Studies of Protein Folding and FunctionDavid Kliger; Fiscal Year: 2007..abstract_text> ..
- SYNTHESES OF HEMES FOR PROTEIN STUDIESKevin Smith; Fiscal Year: 2003..Some methods for such approaches have already been worked out, but improvements and discovery of new methodology, simple and multistep, is planned and anticipated. ..
- SYNTHESES OF HEMES FOR PROTEIN STUDIESKevin Smith; Fiscal Year: 2002..Some methods for such approaches have already been worked out, but improvements and discovery of new methodology, simple and multistep, is planned and anticipated. ..
- OPTICAL PROPERTIES OF BIOLOGICAL MACROMOLECULESROBERT WOODY; Fiscal Year: 2003..Models of multi-heme proteins obtained by protein design (maquettes) will be studied experimentally and theoretically. ..
- OPTICAL PROPERTIES OF BIOLOGICAL MACROMOLECULESROBERT WOODY; Fiscal Year: 2002..Models of multi-heme proteins obtained by protein design (maquettes) will be studied experimentally and theoretically. ..
- NO DYNAMICS IN HEMEPROTEINS--TRANSIENT INFRARED STUDIESMarco Pereira; Fiscal Year: 1999..of the proposed research is the study of the ligand dynamics of carbonmonoxide(CO) nitric oxide (NO) in hemeproteins and the correlated protein conformational changes using transient mid-infrared absorption, picosecond Raman, ..
- REACTIONS OF HEMEPROTEINS AND OXYGENWinslow Caughey; Fiscal Year: 1990Oxygen biochemistry associated with hemeproteins will be investigated...
- CHAPERONES AND PROTEIN BIOSYNTHESIS IN MYELOID CELLSWILLIAM NAUSEEF; Fiscal Year: 2005..Since these two hemeproteins are essential for optimal PMN activity, the biosynthesis of each in the endoplasmic reticulum (ER) is finely ..
- ELECTRONIC STRUCTURE OF FERRIC PORPHYRINSCHARLES STROUSE; Fiscal Year: 1990..As a part of the proposed investigation they will also be used to construct matrices for the study of ligand and solvate dynamics and the study of excitation and electron transfer...
- PSYCHOACTIVE DRUG METABOLISM BY BRAIN CYTOCHROMES P450Vijayalakshmi Ravindranath; Fiscal Year: 1999..Human brain P-450 may influence pharmacological modulation of drugs acting on CNS; it is therefore important to identify P-450 mediated biotransformation of psychoactive drugs and characterize the enzymes mediating such reactions. ..
- The mechanism of immunosuppression by thymosin beta 4.ELENA YARMOLA; Fiscal Year: 2007..As an initial step toward these goals, I specifically propose to investigate the mechanism by which thymosin beta4 is oxidized, secreted, and recognized by cells of the immune system. ..
- HEME PROTEIN STRUCTURE AND FUNCTIONJack Peisach; Fiscal Year: 2004..abstract_text> ..
- Structure Function Relationship in HemeproteinsSyun Ru Yeh; Fiscal Year: 2005..Experiments are proposed to test the functional consequences of this finding. These hemeprotein systems provide an excellent model for investigating fundamental structural properties that underlie biological reactivity. ..
- NOVEL SUBSTRATE OXIDATION BY ENZYME ENGINEERINGDavid B Goodin; Fiscal Year: 2010..In addition, information about the inherent propensity of a particular protein architecture to accept a given ligand should provide insight into fundamentals of P450 substrate specificity. ..
- ACTIVE SITE PROPERTIES OF FE(III) HEME PROTEINSJAMES ERMAN; Fiscal Year: 1991....
- BIOPHYSICAL EVALUATION OF SOL-GEL ENCAPSULATED PROTEINSJoel Friedman; Fiscal Year: 2003..It is anticipated that this comprehensive study will result in an algorithm for matching an encapsulation protocol to a particular biomedical or biophysical application. ..
- BIOPHYSICAL EVALUATION OF SOL-GEL ENCAPSULATED PROTEINSJoel Friedman; Fiscal Year: 2001..It is anticipated that this comprehensive study will result in an algorithm for matching an encapsulation protocol to a particular biomedical or biophysical application. ..
- THEORY OF PHYSICAL PROPERTIES AND REACTIVITY IN P450Danni Harris; Fiscal Year: 2000..Paul Ortiz de Montellano has agreed to make mutants of the 4A1 and 4A11 isozyme suggested by our proposed 3D structure and binding site that will further test the reliability of the models to make robust predictions. ..
- THEORETICAL STUDIES OF LIGAND BINDING TO HEME PROTEINSDavid Case; Fiscal Year: 1990..This will allow comparisons to rate constants determined from laser photodissociation experiments, and will clarify the connection between elements of protein structure and the intermediates seen in kinetics experiments...
- PICOSECOND LASER STUDIES OF HEME-PROTEINSKENNETH EISENTHAL; Fiscal Year: 1980....
- OXYGEN UTILIZING MEMBRANE HEME PROTEINSShelagh Ferguson Miller; Fiscal Year: 2002....
- Resonance Raman Studies of Mammalian Heme ProteinsJames Kincaid; Fiscal Year: 2007..abstract_text> ..
- STRUCTURAL AND DYNAMIC STUDY OF MODEL HEME COMPLEXESGERD LA MAR; Fiscal Year: 1999..The structural probes developed for the model myoglobins will be applied towards elucidating the molecular mechanism of allostery in monomeric, dimeric and tetrameric hemoglobins. ..
- MOLECULAR TARGETS OF NITRIC OXIDE IN CARDIAC TRANSPLANTSGALEN PIEPER; Fiscal Year: 2004..These studies will provide a novel mechanism to explain the pathogenesis of dyskinesis and graft failure during cardiac transplant rejection. ..
- iNOS Posttranslational Regulation in Cardiac RejectionGALEN PIEPER; Fiscal Year: 2009..A better understanding of this molecular process may lead to better strategies to prevent injury cardiac cells in these patients. ..
- RENAL INJURY AND ADAPTATION TO HEME PROTEINSKarl Nath; Fiscal Year: 2001..Our proposed application thus continues to pursue the functionality of HO-1, as it utilized a new strategy, transgenic mice, and as it defines a new area of involvement namely, renal inflammation. ..
- Magnetic Circular Dichroism/Circular Discroism SystemAh Lim Tsai; Fiscal Year: 2008..Dichroism (MCD/CD) system, including JASCO-815 and JASCO-730 spectrophotometers, to be used for a wide range of hemeproteins and other biomedical research...
- Structure-Function and Reacation Mechanism of eNOSAh Lim Tsai; Fiscal Year: 2007..The last Aim will provide understanding of the role of reductase and CaM in regulating the redox coupling with the oxygenase domain thus lead to an elucidation of the reaction of the reaction mechanism of the whole eNOS. ..