hemeproteins

Summary

Summary: Proteins that contain an iron-porphyrin, or heme, prosthetic group resembling that of hemoglobin. (From Lehninger, Principles of Biochemistry, 1982, p480)

Top Publications

  1. pmc Toll-like receptor 9 mediates innate immune activation by the malaria pigment hemozoin
    Cevayir Coban
    Exploratory Research for Advanced Technology ERATO, Japan Science and Technology Agency JST, Osaka, Japan
    J Exp Med 201:19-25. 2005
  2. pmc Fzf1p regulates an inducible response to nitrosative stress in Saccharomyces cerevisiae
    Aaron Sarver
    Chemistry and Chemical Biology Graduate Program, University of California, San Francisco, San Francisco, CA 94143, USA
    Mol Biol Cell 16:4781-91. 2005
  3. pmc Pure Hemozoin is inflammatory in vivo and activates the NALP3 inflammasome via release of uric acid
    Jason W Griffith
    Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06511, USA
    J Immunol 183:5208-20. 2009
  4. ncbi Innate inflammatory response to the malarial pigment hemozoin
    Marina T Shio
    Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada
    Microbes Infect 12:889-99. 2010
  5. pmc Involvement of inflammatory chemokines in survival of human monocytes fed with malarial pigment
    Giuliana Giribaldi
    Department of Genetics, Biology and Biochemistry, University of Torino Medical School, Turin, Italy
    Infect Immun 78:4912-21. 2010
  6. ncbi Adaptations against heme toxicity in blood-feeding arthropods
    Aurelio V Graca-Souza
    Instituto de Bioquimica Medica, Universidade Federal do Rio de Janeiro, Centro de Ciencias da Saude, Av Brigadeiro Trompowsky, S N, Cidade Universitaria, Ilha do Fundao, 21941 690 Rio de Janeiro, RJ, Brazil
    Insect Biochem Mol Biol 36:322-35. 2006
  7. pmc Identification and characterization of the hemophore-dependent heme acquisition system of Yersinia pestis
    M S Rossi
    , 75724 Paris Cedex 15, France
    Infect Immun 69:6707-17. 2001
  8. pmc Natural hemozoin stimulates syncytiotrophoblast to secrete chemokines and recruit peripheral blood mononuclear cells
    N W Lucchi
    Department of Infectious Diseases, University of Georgia, Athens, GA 30602, USA
    Placenta 32:579-85. 2011
  9. ncbi Malaria-parasitized erythrocytes and hemozoin nonenzymatically generate large amounts of hydroxy fatty acids that inhibit monocyte functions
    Evelin Schwarzer
    Institute of Biochemistry, Humboldt University Charite, Berlin, Germany
    Blood 101:722-8. 2003
  10. pmc The role of neutral lipid nanospheres in Plasmodium falciparum haem crystallization
    John M Pisciotta
    Malaria Research Institute, W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, 615 N Wolfe Street, Baltimore, MD 21205, USA
    Biochem J 402:197-204. 2007

Detail Information

Publications303 found, 100 shown here

  1. pmc Toll-like receptor 9 mediates innate immune activation by the malaria pigment hemozoin
    Cevayir Coban
    Exploratory Research for Advanced Technology ERATO, Japan Science and Technology Agency JST, Osaka, Japan
    J Exp Med 201:19-25. 2005
    ..These data suggest that TLR9-mediated, MyD88-dependent, and CQ-sensitive innate immune activation by HZ may play an important role in malaria parasite-host interactions...
  2. pmc Fzf1p regulates an inducible response to nitrosative stress in Saccharomyces cerevisiae
    Aaron Sarver
    Chemistry and Chemical Biology Graduate Program, University of California, San Francisco, San Francisco, CA 94143, USA
    Mol Biol Cell 16:4781-91. 2005
    ..Our results suggest that the presence of *NO or *NO derivatives activates Fzf1p leading to transcriptional induction of a discrete set of target genes that function to protect the cell from *NO-mediated stress...
  3. pmc Pure Hemozoin is inflammatory in vivo and activates the NALP3 inflammasome via release of uric acid
    Jason W Griffith
    Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06511, USA
    J Immunol 183:5208-20. 2009
    ..These data suggest that uric acid is released during malaria infection and may serve to augment the initial host response to hemozoin via activation of the NALP3 inflammasome...
  4. ncbi Innate inflammatory response to the malarial pigment hemozoin
    Marina T Shio
    Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada
    Microbes Infect 12:889-99. 2010
    ..This review will focus on HZ biosynthesis and synthetic preparation, but in particular on its effect on host's innate inflammatory responses...
  5. pmc Involvement of inflammatory chemokines in survival of human monocytes fed with malarial pigment
    Giuliana Giribaldi
    Department of Genetics, Biology and Biochemistry, University of Torino Medical School, Turin, Italy
    Infect Immun 78:4912-21. 2010
    ....
  6. ncbi Adaptations against heme toxicity in blood-feeding arthropods
    Aurelio V Graca-Souza
    Instituto de Bioquimica Medica, Universidade Federal do Rio de Janeiro, Centro de Ciencias da Saude, Av Brigadeiro Trompowsky, S N, Cidade Universitaria, Ilha do Fundao, 21941 690 Rio de Janeiro, RJ, Brazil
    Insect Biochem Mol Biol 36:322-35. 2006
    ..In spite of the existence of a great molecular diversity of protective mechanisms, different hematophagous organisms developed convergent solutions that may be physiologically equivalent...
  7. pmc Identification and characterization of the hemophore-dependent heme acquisition system of Yersinia pestis
    M S Rossi
    , 75724 Paris Cedex 15, France
    Infect Immun 69:6707-17. 2001
    ..This report constitutes the first attempt to investigate the contribution of the hemophore-dependent heme acquisition system in bacterial pathogenicity...
  8. pmc Natural hemozoin stimulates syncytiotrophoblast to secrete chemokines and recruit peripheral blood mononuclear cells
    N W Lucchi
    Department of Infectious Diseases, University of Georgia, Athens, GA 30602, USA
    Placenta 32:579-85. 2011
    ....
  9. ncbi Malaria-parasitized erythrocytes and hemozoin nonenzymatically generate large amounts of hydroxy fatty acids that inhibit monocyte functions
    Evelin Schwarzer
    Institute of Biochemistry, Humboldt University Charite, Berlin, Germany
    Blood 101:722-8. 2003
    ....
  10. pmc The role of neutral lipid nanospheres in Plasmodium falciparum haem crystallization
    John M Pisciotta
    Malaria Research Institute, W Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, 615 N Wolfe Street, Baltimore, MD 21205, USA
    Biochem J 402:197-204. 2007
    ....
  11. pmc Aligned hemozoin crystals in curved clusters in malarial red blood cells revealed by nanoprobe X-ray Fe fluorescence and diffraction
    Sergey Kapishnikov
    Department of Materials and Interfaces, The Weizmann Institute of Science, Rehovot 76100, Israel
    Proc Natl Acad Sci U S A 109:11184-7. 2012
    ..The diffraction results are verified by a study of the nucleation process using emerging tools of three-dimensional cellular microscopy, described in the companion paper...
  12. ncbi The IL-12p70/IL-10 interplay is differentially regulated by free heme and hemozoin in murine bone-marrow-derived macrophages
    Mathieu Cambos
    Department of Biological Sciences, Universite du Quebec a Montreal, Montreal, Que, Canada
    Int J Parasitol 40:1003-12. 2010
    ..Our results suggest that oxidant stress induced by free heme may potentially contribute to sustained production of IL-10 and down-regulation of IL-12 responses in malaria...
  13. pmc Transmembrane heme delivery systems
    B S Goldman
    Department of Biology, Washington University, One Brookings Drive, St Louis, MO 63130, USA
    Proc Natl Acad Sci U S A 95:5003-8. 1998
    ..We believe that a directed heme delivery pathway is vital for the synthesis of cytochromes c, whereby heme iron is protected from oxidation via ligation to histidinyl residues within the delivery proteins...
  14. pmc Haem homeostasis is regulated by the conserved and concerted functions of HRG-1 proteins
    Abbhirami Rajagopal
    Department of Animal and Avian Sciences, University of Maryland, College Park, Maryland 20742, USA
    Nature 453:1127-31. 2008
    ..Thus, uncovering the mechanisms of haem transport in C. elegans may provide insights into human disorders of haem metabolism and reveal new drug targets for developing anthelminthics to combat worm infestations...
  15. pmc Malaria hemozoin is immunologically inert but radically enhances innate responses by presenting malaria DNA to Toll-like receptor 9
    Peggy Parroche
    Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Proc Natl Acad Sci U S A 104:1919-24. 2007
    ..However, its activity depends on being bound to HZ, which we propose amplifies the biological responses to malaria DNA by targeting it to a TLR9(+) intracellular compartment...
  16. ncbi Haemozoin detection in infected erythrocytes for Plasmodium falciparum malaria diagnosis-prospects and limitations
    Maria Rebelo
    Instituto de Medicina Molecular, Faculdade de Medicina, Lisbon, Portugal
    Acta Trop 123:58-61. 2012
    ..Our results strongly suggest that, although it may be useful for monitoring maturation, detection of intra-erythrocytic Hz by flow cytometry will not provide an optimal method for diagnosis of P. falciparum malaria...
  17. pmc A simple and inexpensive haemozoin-based colorimetric method to evaluate anti-malarial drug activity
    Tran Thanh Men
    Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo ku, Kyoto 606 8585, Japan
    Malar J 11:272. 2012
    ..This study describes an inexpensive procedure that is based on the advantage of a positive correlation between the haemozoin level of infected erythrocytes and parasite load...
  18. ncbi On the mechanisms involved in biological heme crystallization
    Renata Stiebler
    Instituto de Bioquimica Medica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil
    J Bioenerg Biomembr 43:93-9. 2011
    ....
  19. pmc Haemozoin induces early cytokine-mediated lysozyme release from human monocytes through p38 MAPK- and NF-kappaB-dependent mechanisms
    Manuela Polimeni
    Dipartimento di Genetica, Biologia e Biochimica, Facolta di Medicina e Chirurgia, Universita degli Studi di Torino, Torino, Italy
    PLoS ONE 7:e39497. 2012
    ....
  20. ncbi Suppression of erythropoiesis in malarial anemia is associated with hemozoin in vitro and in vivo
    Climent Casals-Pascual
    Nuffield Department of Clinical Laboratory Sciences and National Blood Service Oxford Centre, John Radcliffe Hospital, Oxford OX3 9BQ, United Kingdom
    Blood 108:2569-77. 2006
    ..Taken together, these observations provide compelling evidence for inhibition of erythropoiesis by Hz...
  21. pmc The mechanism of direct heme transfer from the streptococcal cell surface protein Shp to HtsA of the HtsABC transporter
    Tyler K Nygaard
    Department of Veterinary Molecular Biology, Montana State University, Bozeman, 59718, USA
    J Biol Chem 281:20761-71. 2006
    ..These results support a direct, rapid, and affinity-driven mechanism of heme and hemin transfer from the cell surface receptor Shp to the ATP-binding cassette transporter system...
  22. pmc The neutral lipid composition present in the digestive vacuole of Plasmodium falciparum concentrates heme and mediates β-hematin formation with an unusually low activation energy
    Anh N Hoang
    Department of Chemistry, Vanderbilt University, Station B351822, Nashville, Tennessee 37235, United States
    Biochemistry 49:10107-16. 2010
    ....
  23. pmc Malaria pigment paralyzes dendritic cells
    Britta C Urban
    Centre for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Clinical Medicine, University of Oxford, Churchill Hospital, Old Road, Oxford, OX3 7LJ, UK
    J Biol 5:4. 2006
    ..A prime suspect, the parasite-produced pigment hemozoin, has now been clearly shown to mediate immunosuppression by inhibiting dendritic cell activity...
  24. pmc Suppression of adaptive immunity to heterologous antigens during Plasmodium infection through hemozoin-induced failure of dendritic cell function
    Owain R Millington
    Division of Immunology, Infection and Inflammation, University of Glasgow, Glasgow G11 6NT, UK
    J Biol 5:5. 2006
    ....
  25. pmc Nitric oxide dioxygenase: an enzymic function for flavohemoglobin
    P R Gardner
    Division of Critical Care Medicine, Children s Hospital Medical Center, and Department of Pediatrics, University of Cincinnati, 3333 Burnet Avenue, Cincinnati, OH 45229, USA
    Proc Natl Acad Sci U S A 95:10378-83. 1998
    ....
  26. ncbi The redox activity of hemoglobins: from physiologic functions to pathologic mechanisms
    Brandon J Reeder
    Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex, England
    Antioxid Redox Signal 13:1087-123. 2010
    ....
  27. ncbi Modulation of cytokine profiles by malaria pigment--hemozoin: role of IL-10 in suppression of proliferative responses of mitogen stimulated human PBMC
    Prakash Deshpande
    National Centre for Cell Science NCCS, NCCS campus, Ganeshkhind, Pune 411007, India
    Cytokine 28:205-13. 2004
    ..Kinetics of IL-10 secretion analyzed up to day 6 in MO/MQ cultures fed with Hz revealed that high levels of IL-10 were secreted during the first 48 h after ingestion and decreased drastically at later time points...
  28. pmc Improved methods for magnetic purification of malaria parasites and haemozoin
    Charles C Kim
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA 94158, USA
    Malar J 9:17. 2010
    ..As these methods are not necessarily optimized for parasite purification, the relationship between magnetic field strength and the quantity and quality of yield during parasite purification was explored...
  29. ncbi Natural haemozoin modulates matrix metalloproteinases and induces morphological changes in human microvascular endothelium
    Mauro Prato
    Dipartimento di Genetica, Biologia e Biochimica, Universita di Torino, Torino, Italy
    Cell Microbiol 13:1275-85. 2011
    ..These findings might help understanding the mechanisms of blood brain barrier damage during CM...
  30. ncbi Evaluation of unbound free heme in plant cells by differential acetone extraction
    Nino A Espinas
    Graduate School of Science, The University of Tokyo, Tokyo, Japan
    Plant Cell Physiol 53:1344-54. 2012
    ..The present data demonstrate that the combination of single-step acetone extraction following a sensitive heme assay is the ideal method for determining total and free heme in plants...
  31. pmc Inhibiting Plasmodium falciparum growth and heme detoxification pathway using heme-binding DNA aptamers
    Jacquin C Niles
    Department of Chemistry, University of California, Berkeley, CA 94720 3220, USA
    Proc Natl Acad Sci U S A 106:13266-71. 2009
    ..Altogether, these data demonstrate that aptamers can be versatile tools with applicability in functionally dissecting important P. falciparum-specific pathways both in vitro and in vivo...
  32. pmc Differential regulation of beta-chemokines in children with Plasmodium falciparum malaria
    Daniel O Ochiel
    Department of Infectious Diseases and Microbiology, University of Pittsburgh, 130 DeSoto Street, 603 Parran Hall, Pittsburgh, Pennsylvania 15261, USA
    Infect Immun 73:4190-7. 2005
    ..Altered patterns of circulating beta-chemokines result, at least in part, from Hz-induced changes in beta-chemokine gene expression in blood mononuclear cells...
  33. ncbi Bacterial heme sources: the role of heme, hemoprotein receptors and hemophores
    C Wandersman
    Unité des Membranes Bactériennes, Institut Pasteur CNRS URA 1300, Paris Cedex 15, 75724, France
    Curr Opin Microbiol 3:215-20. 2000
    ..Recent genetic, biochemical and crystallization studies have started to unravel the mechanism and molecular interactions between heme-carrier proteins and components of bacterial heme assimilation systems...
  34. pmc Simple flow cytometric detection of haemozoin containing leukocytes and erythrocytes for research on diagnosis, immunology and drug sensitivity testing
    Rosangela Frita
    Instituto de Medicina Molecular, Faculdade de Medicina de Lisboa, Lisbon, Hospital Universitario de Santa Maria, Av, Prof, Egas Moniz, P 1649 028 Lisboa, Portugal
    Malar J 10:74. 2011
    ..These can range from the ex-vivo and in-vitro detection and functional analysis of Hz-containing leukocytes to the detection of parasitized Red-Blood-Cells (pRBCs) to assess antimalarial activity...
  35. pmc HDP-a novel heme detoxification protein from the malaria parasite
    Dewal Jani
    Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, Virginia, United States of America
    PLoS Pathog 4:e1000053. 2008
    ..As Hz formation is critical for survival, involvement of HDP in this process suggests that it could be a malaria drug target...
  36. ncbi A colorimetric high-throughput beta-hematin inhibition screening assay for use in the search for antimalarial compounds
    Kanyile K Ncokazi
    Department of Chemistry, University of Cape Town, Private Bag, Rondebosch 7701, South Africa
    Anal Biochem 338:306-19. 2005
    ..The quantitative in vitro antimalarial activities of a set of 13 aminoquinolines and quinoline methanols were found to correlate significantly with beta-hematin inhibition values determined using the assay...
  37. ncbi Flavohaemoglobin HmpX from Erwinia chrysanthemi confers nitrosative stress tolerance and affects the plant hypersensitive reaction by intercepting nitric oxide produced by the host
    Martine Boccara
    Laboratoire de Pathologie Végétale, UMR 217 INRA INAP G Paris VI, 16 rue Claude Bernard, 75005 Paris, France
    Plant J 43:226-37. 2005
    ....
  38. ncbi Role of the NF-κB transcription pathway in the haemozoin- and 15-HETE-mediated activation of matrix metalloproteinase-9 in human adherent monocytes
    Mauro Prato
    Dipartimento di Genetica, Biologia e Biochimica, Universita di Torino, Via Santena 5 bis, 10126 Torino, Italy
    Cell Microbiol 12:1780-91. 2010
    ....
  39. pmc Interference with hemozoin formation represents an important mechanism of schistosomicidal action of antimalarial quinoline methanols
    Juliana B R Corrêa Soares
    Laboratório de Bioquímica Redox, Programa de Biologia Molecular e Biotecnologia, Instituto de Bioquimica Medica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
    PLoS Negl Trop Dis 3:e477. 2009
    ....
  40. pmc Synthetic Plasmodium-like hemozoin activates the immune response: a morphology - function study
    Maritza Jaramillo
    The Research Institute of the McGill University Health Centre, Centre for the Study of Host Resistance, Departments of Medicine, Microbiology and Immunology, McGill University, Montreal, Canada
    PLoS ONE 4:e6957. 2009
    ..Periodic quality control experiments and unification of the method of HZ synthesis are key steps to unravel the role of HZ in malaria immunopathology...
  41. pmc Naturally acquired hemozoin by monocytes promotes suppression of RANTES in children with malarial anemia through an IL-10-dependent mechanism
    Tom Were
    University of New Mexico Kenya Medical Research Institute, Laboratories of Parasitic and Viral Diseases, Centre for Global Health Research, Kisumu, Kenya
    Microbes Infect 11:811-9. 2009
    ..028) in PBMC from children with naturally acquired Hz. Results here demonstrate that monocytic acquisition of Hz suppresses RANTES production in children with MA through an IL-10-dependent mechanism...
  42. pmc Species specificity, surface exposure, protein expression, immunogenicity, and participation in biofilm formation of Porphyromonas gingivalis HmuY
    Teresa Olczak
    Laboratory of Biochemistry, Faculty of Biotechnology, University of Wroclaw, Tamka 2, 50 137 Wroclaw, Poland
    BMC Microbiol 10:134. 2010
    ..The aim of this study was to examine the species specificity, surface exposure, protein expression, immunogenicity, and participation in biofilm formation of the P. gingivalis heme-binding protein HmuY...
  43. ncbi Isolation and characterization of an extracellular haem-binding protein from Pseudomonas aeruginosa that shares function and sequence similarities with the Serratia marcescens HasA haemophore
    S Letoffe
    Unité de Physiologie Cellulaire Institut Pasteur CNRS URA 1300, Paris, France
    Mol Microbiol 28:1223-34. 1998
    ..HasAp, like HasA, lacks a signal peptide and is secreted by an ABC transporter. These findings show that haemophore-dependent haem acquisition is not unique to S. marcescens...
  44. ncbi Heme protein assemblies
    Charles J Reedy
    Department of Chemistry, Columbia University, 3000 Broadway, MC 3121, New York, New York 10027, USA
    Chem Rev 104:617-49. 2004
  45. ncbi Diversity and conservation of interactions for binding heme in b-type heme proteins
    Sabine Schneider
    School of Pharmacy and Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, England, UK
    Nat Prod Rep 24:621-30. 2007
  46. ncbi Mitochondrial cytochrome oxidase produces nitric oxide under hypoxic conditions: implications for oxygen sensing and hypoxic signaling in eukaryotes
    Pablo R Castello
    The Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309, USA
    Cell Metab 3:277-87. 2006
    ....
  47. ncbi Magnetic field enriched surface enhanced resonance Raman spectroscopy for early malaria diagnosis
    Clement Yuen
    Nanyang Technological University, Division of Bioengineering, School of Chemical and Biomedical Engineering, Singapore 637457
    J Biomed Opt 17:017005. 2012
    ....
  48. pmc A magneto-optic route toward the in vivo diagnosis of malaria: preliminary results and preclinical trial data
    Dave M Newman
    School of Engineering, Computing and Mathematics, University of Exeter, Exeter EX4 4QF, United Kingdom
    Biophys J 95:994-1000. 2008
    ....
  49. pmc Identification and characterization of HtsA, a second heme-binding protein made by Streptococcus pyogenes
    Benfang Lei
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
    Infect Immun 71:5962-9. 2003
    ..Our results suggest that HtsABC is an ABC transporter that may participate in heme acquisition in GAS...
  50. pmc Heme uptake across the outer membrane as revealed by crystal structures of the receptor-hemophore complex
    Stefanie Krieg
    Fachbereich Biologie, Universitat Konstanz, 78457 Konstanz, Germany
    Proc Natl Acad Sci U S A 106:1045-50. 2009
    ..Subsequently, steric displacement of heme by a receptor residue ruptures the other axial coordination, leading to heme transfer into the receptor...
  51. ncbi The diversity of globin-coupled sensors
    Tracey Allen K Freitas
    Department of Microbiology, Snyder Hall 207, 2538 The Mall, University of Hawaii, Honolulu, HI 96822, USA
    FEBS Lett 552:99-104. 2003
    ..The GCS superfamily is composed of two major subfamilies: the aerotactic and gene regulators. We postulate the existence of protoglobin in Archaea as the predecessor to the chimeric GCS...
  52. pmc Increase on the initial soluble heme levels in acidic conditions is an important mechanism for spontaneous heme crystallization in vitro
    Renata Stiebler
    Laboratório de Bioquímica Redox, Programa de Biologia Molecular e Biotecnologia, Instituto de Bioquimica Medica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
    PLoS ONE 5:e12694. 2010
    ..Here, we aimed to investigate the role of medium polarity on spontaneous βH formation in vitro...
  53. pmc Computational investigation on the photoacoustics of malaria infected red blood cells
    Ratan K Saha
    Ratan K Saha Applied Material Science Division, Saha Institute of Nuclear Physics, Kolkata, West Bengal, India
    PLoS ONE 7:e51774. 2012
    ..The presence of hemozoin inside the cells was examined and an excellent estimation was made. The simulation results suggest that intraerythrocytic stages of malarial parasite may be assessed using the PA technique...
  54. pmc One ring to rule them all: trafficking of heme and heme synthesis intermediates in the metazoans
    Iqbal Hamza
    Department of Animal and Avian Sciences, University of Maryland, College Park, MD 20742, USA
    Biochim Biophys Acta 1823:1617-32. 2012
    ..Additionally, older, well established data are reexamined in light of more recent studies on cellular organization and data available from newer model organisms. This article is part of a Special Issue entitled: Cell Biology of Metals...
  55. ncbi Platelet accumulation in brain microvessels in fatal pediatric cerebral malaria
    Georges E Grau
    Experimental Parasitology Unit, IFR48, Universite de la Mediterranee, Marseille, France
    J Infect Dis 187:461-6. 2003
    ..Platelet accumulation occurs in the microvasculature of patients with CM and may play a role in the pathogenesis of the disease...
  56. pmc Transcriptional regulation of the heme binding protein gene family of Bartonella quintana is accomplished by a novel promoter element and iron response regulator
    James M Battisti
    Division of Biological Sciences, The University of Montana, Missoula, MT 59812, USA
    Infect Immun 75:4373-85. 2007
    ....
  57. pmc Molecular characterization of the hemin uptake locus (hmu) from Yersinia pestis and analysis of hmu mutants for hemin and hemoprotein utilization
    J M Thompson
    Department of Microbiology and Immunology, University of Kentucky, Lexington, Kentucky 40536 0084, USA
    Infect Immun 67:3879-92. 1999
    ..Growth studies showed that a DeltahmuP'RSTUV mutant could grow in iron-depleted medium containing high concentrations of hemoglobin, suggesting that an Hmu-independent, lower-affinity hemoglobin uptake system may exist...
  58. pmc BhuR, a virulence-associated outer membrane protein of Bordetella avium, is required for the acquisition of iron from heme and hemoproteins
    Erin R Murphy
    The Witebsky Center for Microbial Pathogenesis and Immunology, School of Medicine and Biomedical Sciences, University at Buffalo, State University of New York, Buffalo, New York 14214, USA
    Infect Immun 70:5390-403. 2002
    ..avium to demonstrate that the bacterium transports heme into the cytoplasm in a BhuR-dependent manner. The role of BhuR in virulence was established in turkey poults by use of a competitive-infection model...
  59. pmc A novel way to grow hemozoin-like crystals in vitro and its use to screen for hemozoin inhibiting antimalarial compounds
    Vincent Thomas
    STERIS SA R and D, Fontenay aux Roses, France
    PLoS ONE 7:e41006. 2012
    ..In this report we describe new ways to form hemozoin-like crystals that were incidentally discovered during research in the field of prion inactivation...
  60. ncbi Macrophage inflammatory protein-1alpha mediates matrix metalloproteinase-9 enhancement in human adherent monocytes fed with malarial pigment
    Giuliana Giribaldi
    Dipartimento di Genetica, Biologia e Biochimica, Facolta di Medicina e Chirurgia, Universita degli Studi di Torino, Italy
    Asian Pac J Trop Med 4:925-30. 2011
    ....
  61. pmc Sensitive detection of malaria infection by third harmonic generation imaging
    Jonathan M Belisle
    Biophys J 94:L26-8. 2008
    ..The immense nonlinear response of the intrinsic parasitic by-product pigments suggests that automated optical detection by THG could be used for sensitive and rapid screening of parasite infection in blood samples...
  62. ncbi Molecular characterization of a newly identified heme-binding protein induced during differentiation of urine erythroleukemia cells
    S Taketani
    Department of Hygiene, Kansai Medical University, Moriguchi, Osaka 570 8506, Japan
    J Biol Chem 273:31388-94. 1998
    ..Thus, this newly identified heme-binding protein, p22 HBP, may be involved in heme utilization for hemoprotein synthesis and even be coupled to hemoglobin synthesis during erythroid differentiation...
  63. pmc Ultrafast dynamics of diatomic ligand binding to nitrophorin 4
    Abdelkrim Benabbas
    Department of Physics and Center for Interdisciplinary Research on Complex Systems, Northeastern University, Boston, Massachusetts 02115, USA
    J Am Chem Soc 132:2811-20. 2010
    ..et al. Proc. Natl. Acad. Sci. U.S.A. 2007, 104, 14682)...
  64. ncbi Growth of Plasmodium falciparum induces stage-dependent haemichrome formation, oxidative aggregation of band 3, membrane deposition of complement and antibodies, and phagocytosis of parasitized erythrocytes
    G Giribaldi
    Department of Genetics, Biology and Biochemistry, University of Torino Medical School, Torino, Italy
    Br J Haematol 113:492-9. 2001
    ....
  65. ncbi ABC transporter-mediated release of a haem chaperone allows cytochrome c biogenesis
    Robert E Feissner
    Washington University, Department of Biology Campus Box 1137, 1 Brookings Drive, St Louis, MO 63130, USA
    Mol Microbiol 61:219-31. 2006
    ..We propose that the CcmA2B1C1 complex represents a new subgroup within the ABC transporter superfamily that functions to release a chaperone...
  66. ncbi The Janus nature of heme
    Thomas L Poulos
    Department of Molecular Biology and Biochemistry, University of California, Irvine, California 92697 3900, USA
    Nat Prod Rep 24:504-10. 2007
  67. ncbi Hemozoin and the human monocyte--a brief review of their interactions
    E Schwarzer
    Department of Genetics, Biology and Biochemistry, University of Torino, Via Santena 5 bis, 10126 Torino, Italy
    Parassitologia 50:143-5. 2008
    ..Several HZ effects were reproduced by supplementation with plausible concentrations of HETE or HNE, the first most likely via interaction with PPAR-receptors, the second via adduct or crosslinks formation with critical targets...
  68. pmc Trioxaquines and heme-artemisinin adducts inhibit the in vitro formation of hemozoin better than chloroquine
    Christophe Loup
    Laboratoire de Chimie de Coordination, CNRS, 205 route de Narbonne, 31000, Toulouse Cedex 4, France
    Antimicrob Agents Chemother 51:3768-70. 2007
    ..Trioxaquines, potential antimalarial agents, and heme-artemisinin adducts, resulting from the alkylation of heme by artemisinin, were evaluated as inhibitors of beta-hematin formation in 10 M acetate medium at pH 5...
  69. ncbi The novel extracellular Streptomyces reticuli haem-binding protein HbpS influences the production of the catalase-peroxidase CpeB
    Darío Ortiz de Orué Lucana
    Universitat Osnabruck, FB Biologie Chemie, Barbarastrasse 11, D 49069 Osnabruck, Germany
    Microbiology 150:2575-85. 2004
    ..vi) HbpS shared varying degrees of amino acid identities with bacterial proteins of so far unknown function. This report contributes to the elucidation of the biological function of these proteins...
  70. ncbi Yeast flavohemoglobin protects against nitrosative stress and controls ferric reductase activity
    Anna Lewinska
    Department of Biochemistry and Cell Biology, University of Rzeszow, Rzeszow, Poland
    Redox Rep 11:231-9. 2006
    ..Deletion of the YHB1 gene was found to augment the reduction of Fe(3+) by yeast cells which suggests that flavohemoglobin participates in regulation of the activity of plasma membrane ferric reductase(s)...
  71. pmc Alpha-glucosidase promotes hemozoin formation in a blood-sucking bug: an evolutionary history
    Flávia Borges Mury
    Laboratório de Química e Função de Proteínas e Peptídeos, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Rio de Janeiro, Brazil
    PLoS ONE 4:e6966. 2009
    ..In Rhodnius prolixus, hemoglobin-derived heme is detoxified by biomineralization, forming hemozoin (Hz). Recently, the involvement of the R. prolixus perimicrovillar membranes in Hz formation was demonstrated...
  72. pmc Unique structure and stability of HmuY, a novel heme-binding protein of Porphyromonas gingivalis
    Halina Wojtowicz
    Laboratory of Biochemistry, Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland
    PLoS Pathog 5:e1000419. 2009
    ..It is also stable against thermal and chemical denaturation. In conclusion, these studies reveal novel molecular properties of HmuY that are consistent with its role as a putative virulence factor during bacterial infection...
  73. ncbi Enhanced phagocytosis of ring-parasitized mutant erythrocytes: a common mechanism that may explain protection against falciparum malaria in sickle trait and beta-thalassemia trait
    Kodjo Ayi
    Dipartimento di Genetica, Biologia e Biochimica, Universita di Torino, Italy
    Blood 104:3364-71. 2004
    ....
  74. pmc Artemisinin antimalarials do not inhibit hemozoin formation
    Richard K Haynes
    Antimicrob Agents Chemother 47:1175. 2003
  75. ncbi The hmu locus of Yersinia pestis is essential for utilization of free haemin and haem--protein complexes as iron sources
    J M Hornung
    Department of Microbiology and Immunology, University of Kentucky, Lexington 40536 0084, USA
    Mol Microbiol 20:725-39. 1996
    ....
  76. ncbi A bacterial cytochrome c heme lyase. CcmF forms a complex with the heme chaperone CcmE and CcmH but not with apocytochrome c
    Qun Ren
    Institut fur Mikrobiologie, Eidgenossische Technische Hochschule, Schmelzbergstrasse 7, CH 8092 Zurich, Switzerland
    J Biol Chem 277:7657-63. 2002
    ..We propose that CcmFH forms a bacterial heme lyase complex for the transfer of heme from CcmE to apocytochrome c...
  77. ncbi A simple and rapid colorimetric method to measure hemozoin crystal growth in vitro
    Nguyen Tien Huy
    Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo ku, Kyoto 606 8585, Japan
    Anal Biochem 354:305-7. 2006
  78. pmc Identification of amino acid residues involved in heme binding and hemoprotein utilization in the Porphyromonas gingivalis heme receptor HmuR
    Xinyan Liu
    Department of Medicine, Section of Infectious Diseases, Boston University School of Medicine, 650 Albany St, Boston, MA 02118, USA
    Infect Immun 74:1222-32. 2006
    ..Taken together, these results support a role for H95, H434, and the NPDL motif of the P. gingivalis HmuR protein in heme binding and utilization of serum hemoproteins and the HmuR YRAP motif in serum hemoprotein utilization...
  79. ncbi Heme environment in HmuY, the heme-binding protein of Porphyromonas gingivalis
    Halina Wojtowicz
    Faculty of Biotechnology, Laboratory of Biochemistry, University of Wrocław, Wrocław, Poland
    Biochem Biophys Res Commun 383:178-82. 2009
    ..Analysis of heme binding to several single and double HmuY mutants with the methionine, histidine, cysteine, or tyrosine residues replaced by an alanine residue identified histidines 134 and 166 as potential heme ligands...
  80. ncbi Extracellular lipid droplets promote hemozoin crystallization in the gut of the blood fluke Schistosoma mansoni
    Juliana B R Corrêa Soares
    Instituto de Bioquimica Medica, Programa de Biologia Molecular e Biotecnologia, Universidade Federal do Rio de Janeiro, Cidade Universitaria, Rio de Janeiro, RJ, Brazil
    FEBS Lett 581:1742-50. 2007
    ..mansoni. Thus, these data demonstrate that LDs present in the gut lumen of S. mansoni support Hz formation possibly by allowing association of heme to the lipid-water interface of these structures...
  81. pmc Crystal structure and biochemical features of EfeB/YcdB from Escherichia coli O157: ASP235 plays divergent roles in different enzyme-catalyzed processes
    Xiuhua Liu
    State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China
    J Biol Chem 286:14922-31. 2011
    ..In addition, dramatically enhanced fluorescence excitation and emission of EfeB-PPIX was observed, implying this protein may be used as a red color fluorescence marker...
  82. pmc Simple colorimetric inhibition assay of heme crystallization for high-throughput screening of antimalarial compounds
    Nguyen Tien Huy
    Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo ku, Kyoto 606 8585, Japan
    Antimicrob Agents Chemother 51:350-3. 2007
    ....
  83. pmc Hematological predictors of increased severe anemia in Kenyan children coinfected with Plasmodium falciparum and HIV-1
    Gregory C Davenport
    Department of Infectious Diseases and Microbiology, University of Pittsburgh, Pittsburgh, PA, USA
    Am J Hematol 85:227-33. 2010
    ..Thus, malaria/HIV-1 coinfection is characterized by more profound anemia and increased mortality, with acquisition of monocytic pigment having the most detrimental impact on Hb levels...
  84. ncbi Inhibitory effect of ursolic acid derivatives on hydrogen peroxide- and glutathione-mediated degradation of hemin: a possible additional mechanism of action for antimalarial activity
    Catherine Mullié
    Laboratoire des Glucides UMR CNRS 6219, Faculte de Pharmacie, rue des Louvels, Amienx Cedex 1, France
    Exp Parasitol 125:202-7. 2010
    ..For GSH degradation pathway, grafting of UA structure with a piperazine structure gave the best inhibition, pleading for the implication of this latter moiety in the inhibitory process...
  85. ncbi Characterization of cytosolic tetrapyrrole-binding proteins in Arabidopsis thaliana
    Shigekazu Takahashi
    Department of General Systems Studies, Graduate School of Arts and Sciences, The University of Tokyo, Komaba 3 8 1, Tokyo, 153 8902, Japan
    Photochem Photobiol Sci 7:1216-24. 2008
    ..These results suggest that both cHBP1 and cHBP2 have properties suitable for tetrapyrrole carrier proteins and function in distinct organs in plant cells...
  86. ncbi Plasmodium berghei: in vitro and in vivo activity of dequalinium
    Juan Ricardo Rodrigues
    Unidad de Bioquimica, Facultad de Farmacia, Universidad Central de Venezuela, Apartado 47206, Los Chaguaramos, Caracas 1051 A, Venezuela
    Exp Parasitol 115:19-24. 2007
    ..These results confirm that dequalinium is a promising lead for antimalarial drug development...
  87. ncbi Inhibition assay of beta-hematin formation initiated by lecithin for screening new antimalarial drugs
    Dai Thi Xuan Trang
    Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo ku, Kyoto 606 8585, Japan
    Anal Biochem 349:292-6. 2006
    ..We also indicated that dimethyl sulfoxide is an ideal solvent for preparation of heme stock solution, which is stable and can be used for 1 month...
  88. ncbi Loss of ATP hydrolysis activity by CcmAB results in loss of c-type cytochrome synthesis and incomplete processing of CcmE
    Olaf Christensen
    Institut fur Mikrobiologie, Eidgenossische Technische Hochschule, Zurich, Switzerland
    FEBS J 274:2322-32. 2007
    ..coli with a ccmA deletion did not restore cytochrome c production. Our results suggest a role for CcmAB in the handling of heme by CcmE, which is chemically complex and involves an unusual histidine-heme covalent bond...
  89. pmc Hemin binding protein C is found in outer membrane vesicles and protects Bartonella henselae against toxic concentrations of hemin
    Julie A Roden
    Microbial Pathogenesis and Host Defense Program, Department of Medicine, University of California at San Francisco, San Francisco, California, USA
    Infect Immun 80:929-42. 2012
    ..henselae to infect the cat host. Modulation of hbpC expression appears to be a strategy employed by B. henselae to survive in the arthropod vector and the mammalian host...
  90. pmc Ellagitannins of the fruit rind of pomegranate (Punica granatum) antagonize in vitro the host inflammatory response mechanisms involved in the onset of malaria
    Mario dell'Agli
    Dipartimento di Scienze Farmacologiche, Universita degli Studi di Milano, Via Balzaretti, 9 20133 Milano, Italy
    Malar J 9:208. 2010
    ..The aim of the present study was to explore whether the anti-malarial effect of OMARIA could also be sustained via other mechanisms among those associated to the host immune response...
  91. pmc The HP-1 maquette: from an apoprotein structure to a structured hemoprotein designed to promote redox-coupled proton exchange
    Steve S Huang
    The Johnson Research Foundation and Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 101:5536-41. 2004
    ..Oxidation-reduction of both hemes displays an unusually low midpoint potential (-248 mV vs. normal hydrogen electrode at pH 9.0), which is strongly coupled to proton binding, as designed...
  92. pmc A pivotal heme-transfer reaction intermediate in cytochrome c biogenesis
    Despoina A I Mavridou
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom
    J Biol Chem 287:2342-52. 2012
    ..We propose the complex is a trapped catalytic intermediate in the cytochrome c biogenesis process, at the point of heme transfer from CcmE to the cytochrome, the key step in the maturation pathway...
  93. ncbi Physical interaction of CcmC with heme and the heme chaperone CcmE during cytochrome c maturation
    Q Ren
    Institut fur Mikrobiologie, Eidgenossische Technische Hochschule, Schmelzbergstrasse 7, CH 8092 Zurich, Switzerland
    J Biol Chem 276:32591-6. 2001
    ..We propose that CcmC, CcmE, and heme interact directly with each other, establishing a periplasmic heme delivery pathway for cytochrome c maturation...
  94. ncbi Haem-polypeptide interactions during cytochrome c maturation
    L Thony-Meyer
    Institute of Microbiology, ETH Zurich, Schmelzbergstrasse 7, CH 8092, Zurich, Switzerland
    Biochim Biophys Acta 1459:316-24. 2000
    ..CcmC is required for binding of haem to CcmE, and CcmF for releasing it from CcmE and transferring it onto the apocytochrome. The mechanism by which haem crosses the cytoplasmic membrane is currently unknown...
  95. ncbi Bond or cage effect: how nitrophorins transport and release nitric oxide
    Marcelo A Marti
    Departamento de Quimica Inorganica, Analitica, y Quimica Fisica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, INQUIMAE CONICET, Ciudad Universitaria, Pab 2, C1428EHA, Buenos Aires, Argentina
    J Am Chem Soc 130:1611-8. 2008
    ..In contrast to most heme proteins that control ligand affinity by modulating the bond strength to the iron, NP4 has evolved a cage mechanism that traps the NO at low pH and releases it upon cage opening when the pH rises...
  96. ncbi In situ localization and structural analysis of the malaria pigment hemozoin
    Torsten Frosch
    Institut fur Physikalische Chemie, Friedrich Schiller Universitat Jena, Helmholtzweg 4, D 07743 Jena, Germany
    J Phys Chem B 111:11047-56. 2007
    ....
  97. ncbi Haemozoin: from melatonin pigment to drug target, diagnostic tool, and immune modulator
    Thomas Hanscheid
    Institute of Molecular Medicine, Lisbon Medical College, Lisbon, Portugal
    Lancet Infect Dis 7:675-85. 2007
    ..Finally, haemozoin has a substantial effect on the immune system. Further research is needed to clarify these aspects, many of which are important in clinical practice...
  98. ncbi Multi-frequency high-field EPR study of iron centers in malarial pigments
    Andrzej Sienkiewicz
    Institute of Physics, Polish Academy of Sciences, Al Lotnikow 32 46, 02 668 Warsaw, Poland
    J Am Chem Soc 128:4534-5. 2006
    ..85(1) cm-1, E = 0, g perpendicular = 1.95(1), g parallel = 2.00(1). These results point to the existence of largely axial symmetry of the iron environment in the bulk phase of hemozoin and beta-hematin...
  99. ncbi Abnormal display of PfEMP-1 on erythrocytes carrying haemoglobin C may protect against malaria
    Rick M Fairhurst
    Laboratory of Malaria and Vector Research, Research Technologies Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nature 435:1117-21. 2005
    ..Haemoglobin C might protect against malaria by reducing PfEMP-1-mediated adherence of parasitized erythrocytes, thereby mitigating the effects of their sequestration in the microvasculature...
  100. ncbi Heme proteins--diversity in structural characteristics, function, and folding
    Lorna J Smith
    Department of Chemistry, University of Oxford, Inorganic Chemistry Laboratory, Oxford OX1 3QR, United Kingdom
    Proteins 78:2349-68. 2010
    ..The principles identified here provide a framework for the design of de novo proteins that will exhibit tight heme ligand binding and for the identification of the function of structural genomic target proteins with heme ligands...
  101. pmc Differences in anti-malarial activity of 4-aminoalcohol quinoline enantiomers and investigation of the presumed underlying mechanism of action
    Catherine Mullié
    Laboratoire des Glucides, UMR CNRS 6219, UFR de Pharmacie, 1 rue des Louvels, 80037 Amiens Cedex 1, France
    Malar J 11:65. 2012
    ..Their in vitro activity on Plasmodium falciparum W2 and 3D7 strains is reported here along with their inhibition of β-haematin formation and peroxidative degradation of haemin, two possible mechanisms of action of anti-malarial drugs...

Research Grants64

  1. Nitric Oxide Signaling with Heme Proteins
    William R Montfort; Fiscal Year: 2012
    ..Our results are expected to uncover new approaches for drug discovery targeted to cardiovascular disease, cancer metastasis and related failures in human health. ..
  2. Biosynthetic Protein Models of Heme-copper Oxidases and Nitric Oxide Reductases
    Yi Lu; Fiscal Year: 2013
    ..Therefore the work will make important contributions to healthcare, as it will provide a molecular basis for understanding two enzymes important to human health. ..
  3. RESEARCH CENTER FOR PHARMACOLOGY AND DRUG TOXICOLOGY
    L Jackson Roberts; Fiscal Year: 2010
    ..It is anticipated that the projects proposed will significantly advance our understanding of the pharmacology and biology of eicosanoids and related compounds. ..
  4. Oxygen Sensors and P450 Monooxygenases in Mycobacertium tuberculosis
    Paul R Ortiz de Montellano; Fiscal Year: 2013
    ..tuberculosis. ..
  5. NITRIC OXIDE INTERACTION WITH INSECT NITROPHORINS
    FRANCES ANN WALKER; Fiscal Year: 2012
    ....
  6. New mechanism and regulation of intracellular heme delivery in mammals
    Dennis J Stuehr; Fiscal Year: 2013
    ..Our findings will touch many aspects of human health and disease, including blood formation, drug response, anemia, and inflammation. ..
  7. Myoglobin as a Nitrite Reductase that Regulates Hypoxic Cardiac NO Signaling
    Sruti Shiva; Fiscal Year: 2013
    ..This project will investigate how nitrite and myoglobin work together so that nitrite can be used to protect the heart during a heart attack. ..
  8. Molecular Probes of the Mechanisms of Cytocrhome P450
    JOHN TAYLOR GROVES; Fiscal Year: 2013
    ..abstract_text> ..
  9. HEME PROTEIN REDUCTASES
    Stephen G Sligar; Fiscal Year: 2012
    ..abstract_text> ..
  10. Soluble guanylyl cyclase modulation by endogenous elements and exogenous factors
    Emil Martin; Fiscal Year: 2012
    ..Although there are many examples of hemeproteins inhibited by interaction with NO, sGC is the only hemeproteins which is activated by NO binding...
  11. Elucidating Protein Folding Mechanisms
    Jay R Winkler; Fiscal Year: 2010
    ..A clear understanding of the dynamics of polypeptide conformational motions is the first step on the pathway to new molecules that can help proteins avoid or escape from the incorrect structures that lead to disease. ..
  12. Protein, Enzyme, and Biological Water Dynamics: 2D Vibrational Echo Spectroscopy
    Michael D Fayer; Fiscal Year: 2011
    ..The methodology builds on previous successful applications and developments of state-of-the-art ultrafast infrared laser experiments. ..
  13. Femtosecond Coherence Spectroscopy &Ultrafast Kinetic Study of Heme Proteins
    Paul M Champion; Fiscal Year: 2012
    ..Such non-equilibrium processes will be studied to learn if they allow heme proteins to enhance discrimination between different classes of diatomic ligands. ..
  14. Diversity in Heme Protein Structure and Function
    THOMAS LEWIS POULOS; Fiscal Year: 2013
    ..A new crystal structure of a peroxidase-cytochrome c complex has been solved which has provided a new system for probing inter-protein electron transfer. ..
  15. Homocysteine &Heme: A Role in Thrombosis?
    Jan P Kraus; Fiscal Year: 2012
    ..abstract_text> ..
  16. HTS for Cytochrome C Synthesis Pathways
    Robert G Kranz; Fiscal Year: 2011
    ..Thus, the HTS will also discover new inhibitors of heme biosynthesis, and the PIs group has developed the tools to quickly determine which enzyme (of nine) is inhibited in heme synthesis. ..
  17. Development of photothermal microscopy for biomedical applications
    JEROME C MERTZ; Fiscal Year: 2012
    ..e. non-fluorescent) proteins or molecules in tissue. This technology will be useful for in- vivo imaging research applications and rapid tissue diagnosis in the clinic. ..
  18. Cytochrome c biogenesis
    Robert G Kranz; Fiscal Year: 2013
    ..Results here will unravel the mechanisms underlying heme transport, heme red-ox control, and heme attachment to all prokaryotic and plant c-type cytochromes. ..
  19. Conformational Properties of Cytochrome c in Apoptosis
    EKATERINA PLETNEVA; Fiscal Year: 2013
    ....
  20. Design of Heme Protein-Based Blood Substitutes
    John S Olson; Fiscal Year: 2010
    ....
  21. Raman Studies of Mammalian Cytochromes P450
    JAMES ROBERT KINCAID; Fiscal Year: 2013
    ..The resonance Raman technique being used in this work is one of the most powerful methods to acquire this critical information. ..
  22. Oxidation in Inflammation and Cardiovascular Disease
    Stanley L Hazen; Fiscal Year: 2013
    ..It may also lead to important insights for atherosclerosis risk assessment, diagnosis and therapy. ..
  23. 2012 Oxygen Radicals Gordon Research Conference
    Neil Hogg; Fiscal Year: 2012
    ..The conference brings together the leading scientists in the field to share and discuss their current un-published research findings which will stimulate new endeavors and promote novel ways to improve human health. ..
  24. METABOLISM AND NUTRITION OF IRON AND OTHER TRACE METALS
    PAUL SALTMAN; Fiscal Year: 1993
    ..There is a remarkable coherence among the three research goals in that site-specific binding, oxidation-reduction and chelation play significant roles in cellular metabolism of trace elements...
  25. MPO and NO signaling in neointima formation
    Chunxiang Zhang; Fiscal Year: 2010
    ..The current proposal will identify a novel mediator, leukocyte-derived MPO, in neointima formation and its molecular mechanism(s). Blocking the MPO pathway might be a new therapeutic approach to restenosis after balloon angioplasty. ..
  26. Structural Biology of Gaseous Messenger Signaling
    Sudha Veeraraghavan; Fiscal Year: 2011
    ..This proposal is aimed at understanding how nitric oxide works in healthy and disease states. ..
  27. HNO Interactions with Metalloproteins: Structures and Mechanisms
    Yong Zhang; Fiscal Year: 2013
    ..Results will provide useful structural and mechanistic results of HNO interactions with metalloproteins and models to facilitate studies of health, diseases, and therapeutic treatments involving HNO. ..
  28. Electronic, Structural, and Kinetic Characterizations of Cytochrome P450 Compound
    Michael T Green; Fiscal Year: 2013
    ..Proposed experiments thus provide access to the magnitude of D(O-H), allowing insight into how P450s leverage redox potential and ferryl basicity to activate hydrocarbons. ..
  29. MECHANISMS INVOLVED IN FLAVIN-LINKED OXYGEN METABOLISM
    Al Claiborne; Fiscal Year: 2005
    ..for the biochemical diversity in oxygen metabolism, due to their lack of respiratory cytochromes and other hemeproteins. Instead these organisms rely on networks of unusual flavin-linked respiratory enzymes, and this application is ..
  30. Heme Oxygenase and Spinal Cord Injury
    Linda Noble; Fiscal Year: 2004
    ..abstract_text> ..
  31. HEME PROTEINS, MICROSPHERES AND SYNTHETIC ANALOGS
    KENNETH SUSLICK; Fiscal Year: 2004
    ....
  32. STRUCTURE AND DYNAMICS OF HEME PROTEIN ACTIVE SITES
    JAMES SATTERLEE; Fiscal Year: 2005
    ..We shall proceed using modern protein engineering methods combined with x-ray crystallography, kinetics, photothermal methods, equillibrium dynamics methods and NMR spectroscopy. ..
  33. ELECTROSTATICS AND DYNAMICS IN MYOGLOBIN MUTANTS
    STEVEN BOXER; Fiscal Year: 2003
    ..This will permit a direct comparison of experimental data with predictions from molecular dynamics simulations. ..
  34. OXYGEN SENSING AND SIGNAL TRANSDUCTION
    H Bunn; Fiscal Year: 2003
    ..abstract_text> ..
  35. IRON PORPHYRIN ACTIVATION OF DIOXYGEN/SMALL MOLECULES
    ALAN BALCH; Fiscal Year: 2004
    ....
  36. TIME-RESOLVED MAGNETIC CIRCULAR DICHROISM
    David Kliger; Fiscal Year: 2002
    ..abstract_text> ..
  37. Fast Kinetic Studies of Protein Folding and Function
    David Kliger; Fiscal Year: 2007
    ..abstract_text> ..
  38. SYNTHESES OF HEMES FOR PROTEIN STUDIES
    Kevin Smith; Fiscal Year: 2003
    ..Some methods for such approaches have already been worked out, but improvements and discovery of new methodology, simple and multistep, is planned and anticipated. ..
  39. SYNTHESES OF HEMES FOR PROTEIN STUDIES
    Kevin Smith; Fiscal Year: 2002
    ..Some methods for such approaches have already been worked out, but improvements and discovery of new methodology, simple and multistep, is planned and anticipated. ..
  40. OPTICAL PROPERTIES OF BIOLOGICAL MACROMOLECULES
    ROBERT WOODY; Fiscal Year: 2003
    ..Models of multi-heme proteins obtained by protein design (maquettes) will be studied experimentally and theoretically. ..
  41. OPTICAL PROPERTIES OF BIOLOGICAL MACROMOLECULES
    ROBERT WOODY; Fiscal Year: 2002
    ..Models of multi-heme proteins obtained by protein design (maquettes) will be studied experimentally and theoretically. ..
  42. NO DYNAMICS IN HEMEPROTEINS--TRANSIENT INFRARED STUDIES
    Marco Pereira; Fiscal Year: 1999
    ..of the proposed research is the study of the ligand dynamics of carbonmonoxide(CO) nitric oxide (NO) in hemeproteins and the correlated protein conformational changes using transient mid-infrared absorption, picosecond Raman, ..
  43. REACTIONS OF HEMEPROTEINS AND OXYGEN
    Winslow Caughey; Fiscal Year: 1990
    Oxygen biochemistry associated with hemeproteins will be investigated...
  44. CHAPERONES AND PROTEIN BIOSYNTHESIS IN MYELOID CELLS
    WILLIAM NAUSEEF; Fiscal Year: 2005
    ..Since these two hemeproteins are essential for optimal PMN activity, the biosynthesis of each in the endoplasmic reticulum (ER) is finely ..
  45. ELECTRONIC STRUCTURE OF FERRIC PORPHYRINS
    CHARLES STROUSE; Fiscal Year: 1990
    ..As a part of the proposed investigation they will also be used to construct matrices for the study of ligand and solvate dynamics and the study of excitation and electron transfer...
  46. PSYCHOACTIVE DRUG METABOLISM BY BRAIN CYTOCHROMES P450
    Vijayalakshmi Ravindranath; Fiscal Year: 1999
    ..Human brain P-450 may influence pharmacological modulation of drugs acting on CNS; it is therefore important to identify P-450 mediated biotransformation of psychoactive drugs and characterize the enzymes mediating such reactions. ..
  47. The mechanism of immunosuppression by thymosin beta 4.
    ELENA YARMOLA; Fiscal Year: 2007
    ..As an initial step toward these goals, I specifically propose to investigate the mechanism by which thymosin beta4 is oxidized, secreted, and recognized by cells of the immune system. ..
  48. HEME PROTEIN STRUCTURE AND FUNCTION
    Jack Peisach; Fiscal Year: 2004
    ..abstract_text> ..
  49. Structure Function Relationship in Hemeproteins
    Syun Ru Yeh; Fiscal Year: 2005
    ..Experiments are proposed to test the functional consequences of this finding. These hemeprotein systems provide an excellent model for investigating fundamental structural properties that underlie biological reactivity. ..
  50. NOVEL SUBSTRATE OXIDATION BY ENZYME ENGINEERING
    David B Goodin; Fiscal Year: 2010
    ..In addition, information about the inherent propensity of a particular protein architecture to accept a given ligand should provide insight into fundamentals of P450 substrate specificity. ..
  51. ACTIVE SITE PROPERTIES OF FE(III) HEME PROTEINS
    JAMES ERMAN; Fiscal Year: 1991
    ....
  52. BIOPHYSICAL EVALUATION OF SOL-GEL ENCAPSULATED PROTEINS
    Joel Friedman; Fiscal Year: 2003
    ..It is anticipated that this comprehensive study will result in an algorithm for matching an encapsulation protocol to a particular biomedical or biophysical application. ..
  53. BIOPHYSICAL EVALUATION OF SOL-GEL ENCAPSULATED PROTEINS
    Joel Friedman; Fiscal Year: 2001
    ..It is anticipated that this comprehensive study will result in an algorithm for matching an encapsulation protocol to a particular biomedical or biophysical application. ..
  54. THEORY OF PHYSICAL PROPERTIES AND REACTIVITY IN P450
    Danni Harris; Fiscal Year: 2000
    ..Paul Ortiz de Montellano has agreed to make mutants of the 4A1 and 4A11 isozyme suggested by our proposed 3D structure and binding site that will further test the reliability of the models to make robust predictions. ..
  55. THEORETICAL STUDIES OF LIGAND BINDING TO HEME PROTEINS
    David Case; Fiscal Year: 1990
    ..This will allow comparisons to rate constants determined from laser photodissociation experiments, and will clarify the connection between elements of protein structure and the intermediates seen in kinetics experiments...
  56. PICOSECOND LASER STUDIES OF HEME-PROTEINS
    KENNETH EISENTHAL; Fiscal Year: 1980
    ....
  57. OXYGEN UTILIZING MEMBRANE HEME PROTEINS
    Shelagh Ferguson Miller; Fiscal Year: 2002
    ....
  58. Resonance Raman Studies of Mammalian Heme Proteins
    James Kincaid; Fiscal Year: 2007
    ..abstract_text> ..
  59. STRUCTURAL AND DYNAMIC STUDY OF MODEL HEME COMPLEXES
    GERD LA MAR; Fiscal Year: 1999
    ..The structural probes developed for the model myoglobins will be applied towards elucidating the molecular mechanism of allostery in monomeric, dimeric and tetrameric hemoglobins. ..
  60. MOLECULAR TARGETS OF NITRIC OXIDE IN CARDIAC TRANSPLANTS
    GALEN PIEPER; Fiscal Year: 2004
    ..These studies will provide a novel mechanism to explain the pathogenesis of dyskinesis and graft failure during cardiac transplant rejection. ..
  61. iNOS Posttranslational Regulation in Cardiac Rejection
    GALEN PIEPER; Fiscal Year: 2009
    ..A better understanding of this molecular process may lead to better strategies to prevent injury cardiac cells in these patients. ..
  62. RENAL INJURY AND ADAPTATION TO HEME PROTEINS
    Karl Nath; Fiscal Year: 2001
    ..Our proposed application thus continues to pursue the functionality of HO-1, as it utilized a new strategy, transgenic mice, and as it defines a new area of involvement namely, renal inflammation. ..
  63. Magnetic Circular Dichroism/Circular Discroism System
    Ah Lim Tsai; Fiscal Year: 2008
    ..Dichroism (MCD/CD) system, including JASCO-815 and JASCO-730 spectrophotometers, to be used for a wide range of hemeproteins and other biomedical research...
  64. Structure-Function and Reacation Mechanism of eNOS
    Ah Lim Tsai; Fiscal Year: 2007
    ..The last Aim will provide understanding of the role of reductase and CaM in regulating the redox coupling with the oxygenase domain thus lead to an elucidation of the reaction of the reaction mechanism of the whole eNOS. ..