sp1 transcription factor


Summary: Promoter-specific RNA polymerase II transcription factor that binds to the GC box, one of the upstream PROMOTER elements, in mammalian cells. The binding of Sp1 is necessary for the initiation of transcription in the promoters of a variety of cellular and viral GENES.

Top Publications

  1. pmc Senescence mediates pituitary hypoplasia and restrains pituitary tumor growth
    Vera Chesnokova
    Department of Medicine, Cedars Sinai Medical Center, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90048, USA
    Cancer Res 67:10564-72. 2007
  2. ncbi The role of Sp1 and Sp3 in normal and cancer cell biology
    Lin Li
    Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Manitoba R3E 0V9, Canada
    Ann Anat 192:275-83. 2010
  3. pmc Sp1- and Krüppel-like transcription factors
    Joanna Kaczynski
    Gastroenterology Research Unit, Mayo Clinic, Rochester, MN 55901, USA
    Genome Biol 4:206. 2003
  4. ncbi Transcriptional regulation of the Vascular Endothelial Growth Factor gene--a concert of activating factors
    Gilles Pages
    Institute of Signaling, Developmental Biology and Cancer Research, CNRS UMR 6543, Centre Antoine Lacassagne, 33 Avenue de Valombrose, 06189 Nice Cedex 2, France
    Cardiovasc Res 65:564-73. 2005
  5. ncbi A single nucleotide polymorphism in the MDM2 promoter attenuates the p53 tumor suppressor pathway and accelerates tumor formation in humans
    Gareth L Bond
    Cancer Institute of New Jersey, University of Medicine and Dentistry of New Jersey, New Brunswick, NJ 08903, USA
    Cell 119:591-602. 2004
  6. ncbi Significant biological role of sp1 transactivation in multiple myeloma
    Mariateresa Fulciniti
    Jerome Lipper Multiple Myeloma Center, Dana Farber Cancer Institute, Boston, Massachusetts, USA
    Clin Cancer Res 17:6500-9. 2011
  7. pmc Mithramycin is a gene-selective Sp1 inhibitor that identifies a biological intersection between cancer and neurodegeneration
    Sama F Sleiman
    Burke Medical Research Institute, White Plains, New York 10605, USA
    J Neurosci 31:6858-70. 2011
  8. ncbi IL-10 gene expression is controlled by the transcription factors Sp1 and Sp3
    M Tone
    Sir William Dunn School of Pathology, University of Oxford, United Kingdom
    J Immunol 165:286-91. 2000
  9. pmc Myc represses the p21(WAF1/CIP1) promoter and interacts with Sp1/Sp3
    A L Gartel
    Department of Molecular Genetics, University of Illinois College of Medicine, Chicago, IL 60607, USA
    Proc Natl Acad Sci U S A 98:4510-5. 2001
  10. pmc Drugs that target specificity proteins downregulate epidermal growth factor receptor in bladder cancer cells
    Gayathri Chadalapaka
    Department of Veterinary Physiology and Pharmacology, Texas A and M University, 4466 TAMU, Veterinary Research Building 410, College Station, TX 77843 4466, USA
    Mol Cancer Res 8:739-50. 2010

Detail Information

Publications286 found, 100 shown here

  1. pmc Senescence mediates pituitary hypoplasia and restrains pituitary tumor growth
    Vera Chesnokova
    Department of Medicine, Cedars Sinai Medical Center, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90048, USA
    Cancer Res 67:10564-72. 2007
  2. ncbi The role of Sp1 and Sp3 in normal and cancer cell biology
    Lin Li
    Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Manitoba R3E 0V9, Canada
    Ann Anat 192:275-83. 2010
    ..In this review, the role of Sp1 and Sp3 in normal and cancer cell biology and the multiple mechanisms deciding the functional roles of Sp1 and Sp3 will be presented...
  3. pmc Sp1- and Krüppel-like transcription factors
    Joanna Kaczynski
    Gastroenterology Research Unit, Mayo Clinic, Rochester, MN 55901, USA
    Genome Biol 4:206. 2003
    ..Sp1-like/KLF proteins have highly conserved carboxy-terminal zinc-finger domains that function in DNA binding. The amino terminus, containing the transcription activation domain, can vary significantly between family members...
  4. ncbi Transcriptional regulation of the Vascular Endothelial Growth Factor gene--a concert of activating factors
    Gilles Pages
    Institute of Signaling, Developmental Biology and Cancer Research, CNRS UMR 6543, Centre Antoine Lacassagne, 33 Avenue de Valombrose, 06189 Nice Cedex 2, France
    Cardiovasc Res 65:564-73. 2005
  5. ncbi A single nucleotide polymorphism in the MDM2 promoter attenuates the p53 tumor suppressor pathway and accelerates tumor formation in humans
    Gareth L Bond
    Cancer Institute of New Jersey, University of Medicine and Dentistry of New Jersey, New Brunswick, NJ 08903, USA
    Cell 119:591-602. 2004
    ..In humans, SNP309 is shown to associate with accelerated tumor formation in both hereditary and sporadic cancers. A model is proposed whereby SNP309 serves as a rate-limiting event in carcinogenesis...
  6. ncbi Significant biological role of sp1 transactivation in multiple myeloma
    Mariateresa Fulciniti
    Jerome Lipper Multiple Myeloma Center, Dana Farber Cancer Institute, Boston, Massachusetts, USA
    Clin Cancer Res 17:6500-9. 2011
    ..We here provide experimental and clinical evidence that Sp1 plays an important regulatory role in multiple myeloma (MM) cell growth and survival...
  7. pmc Mithramycin is a gene-selective Sp1 inhibitor that identifies a biological intersection between cancer and neurodegeneration
    Sama F Sleiman
    Burke Medical Research Institute, White Plains, New York 10605, USA
    J Neurosci 31:6858-70. 2011
    ..These results support a model in which cancer cell transformation shares key genetic components with neurodegeneration...
  8. ncbi IL-10 gene expression is controlled by the transcription factors Sp1 and Sp3
    M Tone
    Sir William Dunn School of Pathology, University of Oxford, United Kingdom
    J Immunol 165:286-91. 2000
    ..The requirement for Sp1 and Sp3 in transcription of IL-10 was confirmed using Drosophila SL2 cells, which lack endogenous Sp factors. These results suggest that the transcription of IL-10 is positively regulated by both Sp1 and Sp3...
  9. pmc Myc represses the p21(WAF1/CIP1) promoter and interacts with Sp1/Sp3
    A L Gartel
    Department of Molecular Genetics, University of Illinois College of Medicine, Chicago, IL 60607, USA
    Proc Natl Acad Sci U S A 98:4510-5. 2001
    ..Repression of the p21 promoter may contribute to the ability of c-Myc to promote cell proliferation...
  10. pmc Drugs that target specificity proteins downregulate epidermal growth factor receptor in bladder cancer cells
    Gayathri Chadalapaka
    Department of Veterinary Physiology and Pharmacology, Texas A and M University, 4466 TAMU, Veterinary Research Building 410, College Station, TX 77843 4466, USA
    Mol Cancer Res 8:739-50. 2010
    ..Thus, compounds such as curcumin and BA that downregulate Sp transcription factors represent a novel class of anticancer drugs that target EGFR in bladder cancer cells and tumors by inhibiting receptor expression...
  11. pmc Smad2, Smad3 and Smad4 cooperate with Sp1 to induce p15(Ink4B) transcription in response to TGF-beta
    X H Feng
    Departments of Growth and Development and Anatomy, and Programs in Cell Biology and Developmental Biology, University of California, San Francisco, CA 94143 0640, USA
    EMBO J 19:5178-93. 2000
    ..These findings explain the tumor suppressor roles of Smad2 and Smad4 in growth arrest signaling by TGF-beta...
  12. ncbi Constitutive Sp1 activity is essential for differential constitutive expression of vascular endothelial growth factor in human pancreatic adenocarcinoma
    Q Shi
    Department of Gastrointestinal Medical Oncology, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX 77030, USA
    Cancer Res 61:4143-54. 2001
    ..Collectively, our data demonstrated that constitutive Sp1 activation is essential for the differential overexpression of VEGF, which in turn plays an important role in the angiogenesis and progression of human pancreatic cancer...
  13. ncbi Sumoylation of specificity protein 1 augments its degradation by changing the localization and increasing the specificity protein 1 proteolytic process
    Yi Ting Wang
    Institute of Basic Medical Sciences, National Cheng Kung University, Tainan 701, Taiwan
    J Mol Biol 380:869-85. 2008
    ..This interaction increased the efficiency of Sp1 proteolytic processing and ubiquitination and then resulted in Sp1 degradation. Therefore, sumoylation of Sp1 is attenuated during tumorigenesis in order to increase Sp1 stability...
  14. pmc Sp1 cooperates with c-Myc to activate transcription of the human telomerase reverse transcriptase gene (hTERT)
    S Kyo
    Department of Obstetrics and Gynecology, Kanazawa University, School of Medicine, 13 1 Takaramachi, Kanazawa, Ishikawa 920 0934, Japan
    Nucleic Acids Res 28:669-77. 2000
    ..These findings suggest that c-Myc and Sp1 cooperatively function as the major determinants of hTERT expression, and that the switching functions of Myc/Max and Mad/Max might also play roles in telomerase regulation...
  15. pmc Inhibition of NFkappaB and pancreatic cancer cell and tumor growth by curcumin is dependent on specificity protein down-regulation
    Indira Jutooru
    Department of Veterinary Physiology and Pharmacology, Texas A and M University, College Station, Texas 77843, USA
    J Biol Chem 285:25332-44. 2010
  16. ncbi Sp1 and TAFII130 transcriptional activity disrupted in early Huntington's disease
    Anthone W Dunah
    Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Center for Aging, Genetics and Neurodegeneration, Charlestown, MA 02129, USA
    Science 296:2238-43. 2002
    ..Understanding these early molecular events in HD may provide an opportunity to interfere with the effects of mutant huntingtin before the development of disease symptoms...
  17. doi Sp1: emerging roles--beyond constitutive activation of TATA-less housekeeping genes
    Inken Wierstra
    Biochem Biophys Res Commun 372:1-13. 2008
  18. pmc The tumor suppressor p53 and histone deacetylase 1 are antagonistic regulators of the cyclin-dependent kinase inhibitor p21/WAF1/CIP1 gene
    Gerda Lagger
    Institute of Medical Biochemistry, Division of Molecular Biology, Vienna Biocenter, University of Vienna, A 1030 Vienna, Austria
    Mol Cell Biol 23:2669-79. 2003
  19. ncbi The MDM2 promoter SNP285C/309G haplotype diminishes Sp1 transcription factor binding and reduces risk for breast and ovarian cancer in Caucasians
    Stian Knappskog
    Section of Oncology, Institute of Medicine, University of Bergen, Bergen, Norway
    Cancer Cell 19:273-82. 2011
    ..74; CI 0.58-0.94) and breast cancer (OR 0.79; CI 0.62-1.00) among SNP309G carriers...
  20. pmc Phosphorylation by c-Jun NH2-terminal kinase 1 regulates the stability of transcription factor Sp1 during mitosis
    Jian Ying Chuang
    Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan
    Mol Biol Cell 19:1139-51. 2008
    ..Together, our results indicate that JNK1 activation is necessary to phosphorylate Sp1 and to shield Sp1 from the ubiquitin-dependent degradation pathway during mitosis in tumor cell lines...
  21. ncbi Transcriptional regulation of the human DNA polymerase delta catalytic subunit gene POLD1 by p53 tumor suppressor and Sp1
    B Li
    Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595, USA
    J Biol Chem 276:29729-39. 2001
    ..Finally, gel shift assays suggested that p53 competes with Sp1 protein for binding to the P4 sequence of the POLD1 promoter...
  22. ncbi Induction of cPLA2 in lung epithelial cells and non-small cell lung cancer is mediated by Sp1 and c-Jun
    S A Blaine
    Department of Medicine and Pharmacology, University of Colorado Health Science Center, Denver, Colorado 80262, USA
    J Biol Chem 276:42737-43. 2001
    ..Expression of Ha-Ras led to induction of c-Jun protein, which showed functional cooperation with Sp1 in driving promoter activity. Additional unidentified transcription factors bound to the regions from -55/-53 and -37/-34...
  23. ncbi Signaling components involved in Bcl-w-induced migration of gastric cancer cells
    In Hwa Bae
    Laboratory of Radiation Cancer Biology, Korea Institute of Radiological and Medical Sciences, 215 4 Gongneung dong, Nowon Gu, Seoul, South Korea
    Cancer Lett 277:22-8. 2009
    ..These findings significantly advance our understandings of the Bcl-w-induced signaling processes that results in the migration and invasion of cancer cells...
  24. ncbi GATA-4 and GATA-6 modulate tissue-specific transcription of the human gene for P450c17 by direct interaction with Sp1
    Christa E Flück
    Department of Pediatrics, Building MR IV, Room 207, University of California San Francisco, San Francisco, California 94143 0978, USA
    Mol Endocrinol 18:1144-57. 2004
    ..Thus, GATA-4 or GATA-6 and Sp1 together regulate expression of P450c17 in adrenal NCI-H295A cells and methylation of P450c17, GATA-4 and GATA-6 silence the expression of P450c17 in placental JEG-3 cells...
  25. ncbi Yin Yang-1 inhibits vascular smooth muscle cell growth and intimal thickening by repressing p21WAF1/Cip1 transcription and p21WAF1/Cip1-Cdk4-cyclin D1 assembly
    Fernando S Santiago
    Centre for Vascular Research, Department of Pathology, University of New South Wales, and Department of Haematology, The Prince of Wales Hospital, Sydney, NSW, Australia
    Circ Res 101:146-55. 2007
    ..These findings define a new role for YY1 as both an inducer of p53 instability in smooth muscle cells, and an indirect repressor of p21(WAF1/Cip1) transcription, p21(WAF1/Cip1)-cdk4-cyclin D1 assembly and intimal thickening...
  26. ncbi Specific sequence configurations of HIV-1 LTR G/C box array result in altered recruitment of Sp isoforms and correlate with disease progression
    Michael R Nonnemacher
    Department of Microbiology and Immunology, Institute for Molecular Medicine and Infectious Disease, Drexel University College of Medicine, Philadelphia, PA 19129, USA
    J Neuroimmunol 157:39-47. 2004
    ..These results suggest LTR Sp binding site sequence variants may prove useful as viral molecular markers indicative of progressive HIV-1-induced disease...
  27. ncbi Altered expression of transcription factor Sp1 critically impacts the angiogenic phenotype of human gastric cancer
    Liwei Wang
    Shanghai East Hospital Cancer Center, Tongji University, Shanghai, The People s Republic of China
    Clin Exp Metastasis 22:205-13. 2005
    ..Therefore, we provided both clinical and experimental evidence to indicate that Sp1 might impact gastric cancer development and progression through regulating angiogenesis, a critical aspect of cancer biology...
  28. ncbi Sp1 is required for transforming growth factor-beta-induced mesenchymal transition and migration in pancreatic cancer cells
    Kerstin Jungert
    Department of Gastroenterology, University of Ulm, 35043 Ulm, Germany
    Cancer Res 67:1563-70. 2007
    ..It suggests that Sp1, via transcriptional induction of vimentin, cooperates with activated Smad complexes in mesenchymal transition and migration of pancreatic cancer cells upon TGF-beta stimulation...
  29. pmc Sp1 transcription factor and GATA1 cis-acting elements modulate testis-specific expression of mouse cyclin A1
    Sunil K Panigrahi
    Department of Genetics and Development, Columbia University Medical Center, New York, New York, United States of America
    PLoS ONE 7:e47862. 2012
  30. pmc Indole-3-carbinol downregulation of telomerase gene expression requires the inhibition of estrogen receptor-alpha and Sp1 transcription factor interactions within the hTERT promoter and mediates the G1 cell cycle arrest of human breast cancer cells
    Crystal N Marconett
    Department of Molecular and Cell Biology and The Cancer Research Laboratory, University of California, Berkeley, CA 94720 3200, USA
    Carcinogenesis 32:1315-23. 2011
    ..receptor-alpha (ERα) and cyclin-dependent kinase-6 transcripts levels (which is regulated through the Sp1 transcription factor) prior to the downregulation of hTERT suggesting a mechanistic link...
  31. ncbi Sp1 is a transcription repressor to stanniocalcin-1 expression in TSA-treated human colon cancer cells, HT29
    Alice Y S Law
    Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong
    J Cell Biochem 112:2089-96. 2011
    ..Re-ChIP assay illustrated that Sp1-mediated inhibition of STC1 transcription was associated with the recruitment of another repressor molecule, Rb. Collectively our findings identify STC1 is a downstream target of Sp1...
  32. pmc Protein kinase Calpha-induced derepression of the human luteinizing hormone receptor gene transcription through ERK-mediated release of HDAC1/Sin3A repressor complex from Sp1 sites
    Mingjuan Liao
    Program in Developmental Endocrinology and Genetics, National Institutes of Health, Bethesda, Maryland 20892 4510, USA
    Mol Endocrinol 22:1449-63. 2008
  33. ncbi Clinical significance of CD99 down-regulation in gastric adenocarcinoma
    Jung Hyun Lee
    Department of Pharmacy, Research Institute of Pharmaceutical Science, Seoul National University College of Pharmacy, Seoul, Korea
    Clin Cancer Res 13:2584-91. 2007
    ..CD99 is a cell adhesion molecule associated with human tumors. The aim of the present study was to characterize its role in the development and progression of human gastric adenocarcinoma...
  34. pmc Artemisinin blocks prostate cancer growth and cell cycle progression by disrupting Sp1 interactions with the cyclin-dependent kinase-4 (CDK4) promoter and inhibiting CDK4 gene expression
    Jamin A Willoughby
    Department of Molecular and Cell Biology and Cancer Research Laboratory, University of California at Berkeley, Berkeley, California 94720 3200, USA
    J Biol Chem 284:2203-13. 2009
    ..site in the CDK4 promoter forms a specific artemisinin-responsive DNA-protein complex that contains the Sp1 transcription factor. Artemisinin reduced phosphorylation of Sp1, and when dephosphorylation of Sp1 was inhibited by treatment ..
  35. ncbi Functional significance of a putative sp1 transcription factor binding site in the survivin gene promoter
    M V Mityaev
    Shemyakin Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia
    Biochemistry (Mosc) 73:1183-91. 2008
    ..We also demonstrated the functional significance of a putative Sp1 transcription factor-binding site at (-63...-54) upstream from the transcription initiation site...
  36. ncbi Overexpression of HDAC1 induces cellular senescence by Sp1/PP2A/pRb pathway
    Jian Ying Chuang
    Department of Pharmacology, National Cheng Kung University, Tainan 701, Taiwan
    Biochem Biophys Res Commun 407:587-92. 2011
    ..Taken together, these results indicate that HDAC1 overexpression restrained cell proliferation and induced premature senescence in cervical cancer cells through a novel Sp1/PP2A/pRb pathway...
  37. ncbi Sodium butyrate upregulates expression of NKG2D ligand MICA/B in HeLa and HepG2 cell lines and increases their susceptibility to NK lysis
    Cai Zhang
    Institute of Immunopharmacology and Immunotherapy, School of Pharmaceutical Sciences, Shandong University, 44 Wenhua West Road, Jinan, China
    Cancer Immunol Immunother 58:1275-85. 2009
    ..These results suggest that sodium butyrate and other HDAC inhibitors may have therapeutic potential by enhancing the immune response to cancer...
  38. ncbi Domains of estrogen receptor alpha (ERalpha) required for ERalpha/Sp1-mediated activation of GC-rich promoters by estrogens and antiestrogens in breast cancer cells
    Kyounghyun Kim
    Department of Veterinary Physiology and Pharmacology, Texas A and M University, College Station, Texas 77843 4466, USA
    Mol Endocrinol 17:804-17. 2003
  39. pmc Interaction of Huntington disease protein with transcriptional activator Sp1
    Shi Hua Li
    Department of Human Genetics, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Mol Cell Biol 22:1277-87. 2002
    ..These findings suggest that the soluble form of mutant huntingtin in the nucleus may cause cellular dysfunction by binding to Sp1 and thus reducing the expression of Sp1-regulated genes...
  40. ncbi Functional and sequence analysis of human neuroglobin gene promoter region
    Wei Zhang
    School of Biological Sciences, The University of Hong Kong, Hong Kong SAR
    Biochim Biophys Acta 1809:236-44. 2011
    ..Treatment of two non-neuronal cell lines HeLa and BEAS-2B with 5-aza-2'-deoxycytidine remarkably induced NGB expression, suggesting a potential role of DNA methylation in regulating NGB tissue-specific expression...
  41. ncbi 8-Chloro-adenosine-induced E2F1 promotes p14ARF gene activation in H1299 cells through displacing Sp1 from multiple overlapping E2F1/Sp1 sites
    Hai Jun Zhang
    Department of Biochemistry and Molecular Biology, Peking University Health Science Center, Xue Yuan Road 38, Beijing 100083, PR China
    J Cell Biochem 106:464-72. 2009
  42. pmc Sp1-mediated TRAIL induction in chemosensitization
    Jing Xu
    Program in Molecular Biology and Genetics, Department of Pathology, Karmanos Cancer Institute, Wayne State University School of Medicine, Detroit, Michigan 48201, USA
    Cancer Res 68:6718-26. 2008
  43. pmc Latency-associated nuclear antigen of Kaposi's sarcoma-associated herpesvirus up-regulates transcription of human telomerase reverse transcriptase promoter through interaction with transcription factor Sp1
    Subhash C Verma
    Department of Microbiology and Abramson Comprehensive Cancer Center, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    J Virol 78:10348-59. 2004
    ..Together, our results suggest that the interaction of LANA with Sp1 up-regulates the telomerase promoter, potentially contributing to the immortalization of KSHV-infected cells...
  44. ncbi Prostaglandin E2 stimulates human lung carcinoma cell growth through induction of integrin-linked kinase: the involvement of EP4 and Sp1
    Ying Zheng
    Department of Medicine, Division of Pulmonary, Emory University School of Medicine, Atlanta, USA
    Cancer Res 69:896-904. 2009
    ..These studies suggest a novel molecular mechanism by which PGE(2) stimulates NSCLC cell growth and unveils a new molecular target for the development of therapies against NSCLC...
  45. ncbi Sp1-like transcription factors are regulators of embryonic development in vertebrates
    Chengtian Zhao
    Laboratory of Developmental Biology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China
    Dev Growth Differ 47:201-11. 2005
    ..This review mainly summarises their expression patterns and functions during vertebrate embryogenesis...
  46. ncbi Sp1 plays a critical role in the transcriptional activation of the human cyclin-dependent kinase inhibitor p21(WAF1/Cip1) gene by the p53 tumor suppressor protein
    G Koutsodontis
    Department of Basic Sciences, University of Crete Medical School, Heraklion GR-71110, Greece
    J Biol Chem 276:29116-25. 2001
    ..In conclusion, Sp1 must play a critical role in regulating important biological processes controlled by p53 via p21 gene activation such as DNA repair, cell growth, differentiation, and apoptosis...
  47. ncbi PPARalpha activators inhibit vascular endothelial growth factor receptor-2 expression by repressing Sp1-dependent DNA binding and transactivation
    Markus Meissner
    Department of Dermatology, Klinikum der J W Goethe Universitat, Frankfurt am Main, Germany
    Circ Res 94:324-32. 2004
    ..are conveyed by an element located between base pairs -60 and -37 that contains two adjacent consensus Sp1 transcription factor binding sites...
  48. ncbi Sp1: regulation of gene expression by phosphorylation
    Shijian Chu
    McGuire VA Medical Center, Richmond, VA 23249, USA
    Gene 348:1-11. 2005
  49. pmc Regulation of Sp1 by cell cycle related proteins
    Alicia Tapias
    Department of Biochemistry and Molecular Biology, School of Pharmacy, IBUB, University of Barcelona, Barcelona, Spain
    Cell Cycle 7:2856-67. 2008
    b>Sp1 transcription factor regulates the expression of multiple genes, including the Sp1 gene itself. We analyzed the ability of different cell cycle regulatory proteins to interact with Sp1 and to affect Sp1 promoter activity...
  50. ncbi HDAC4 represses p21(WAF1/Cip1) expression in human cancer cells through a Sp1-dependent, p53-independent mechanism
    D Mottet
    Metastasis Research Laboratory, GIGA Cancer Center for Experimental Cancer Research, University of Liege, Liege, Belgium
    Oncogene 28:243-56. 2009
    ..Thus, HDAC4 could be a useful target for new anti-cancer therapies based on selective inhibition of specific HDACs...
  51. pmc Sp1 and p73 activate PUMA following serum starvation
    Lihua Ming
    Department of Pathology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    Carcinogenesis 29:1878-84. 2008
    ..Our results suggest that transcription factors Sp1 and p73 mediate p53-independent induction of PUMA following serum starvation to trigger apoptosis in human cancer cells...
  52. ncbi Overexpression of Sp1 leads to p53-dependent apoptosis in cancer cells
    Jian Ying Chuang
    Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan
    Int J Cancer 125:2066-76. 2009
    ..In conclusion, p53 was an essential factor for Sp1 overexpression-induced apoptotic cell death in transforming cells...
  53. pmc In vitro and in vivo binding of human immunodeficiency virus type 1 Tat protein and Sp1 transcription factor
    K T Jeang
    Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892
    J Virol 67:6224-33. 1993
    ..Assay of material that bound to and eluted from the Tat matrices revealed the presence of the Sp1 transcription factor. Other transcription factors (Oct and NF-kappa B) also bound to Tat matrices but with less efficiency--in ..
  54. ncbi Structural analysis and promoter characterization of the human membrane-type matrix metalloproteinase-1 (MT1-MMP) gene
    J Lohi
    Department of Virology, Haartman Institute, University of Helsinki, Finland
    Gene 242:75-86. 2000
  55. pmc Sp1 is involved in Akt-mediated induction of VEGF expression through an HIF-1-independent mechanism
    Nabendu Pore
    Department of Radiation Oncology, University of Pennsylvania School of Medicine, Philadelphia, PA 19004, USA
    Mol Biol Cell 15:4841-53. 2004
    ..Others have proposed that the PI3K/Akt pathway can increase VEGF expression via the hypoxia-inducible factor 1 (HIF-1); however, our results suggest an alternative mechanism can also operate...
  56. ncbi Transcription factor Sp1 regulates expression of cancer-associated molecule CD147 in human lung cancer
    Ling min Kong
    Department of Cell Biology, Fourth Military Medical University, Xi an, China
    Cancer Sci 101:1463-70. 2010
    ..831). Collectively, our results suggest that Sp1 is essential for regulating the CD147 gene expression in human lung cancer...
  57. ncbi Targeting Sp1 transcription factors in prostate cancer therapy
    Umesh T Sankpal
    Cancer Research Institute, MD Anderson Cancer Center Orlando, Orlando, FL 32827, USA
    Med Chem 7:518-25. 2011
    ..This review will describe the association of Sp proteins in prostate cancer with a special emphasis on some of the agents tested to target Sp proteins for the treatment of this malignancy...
  58. pmc Different members of the Sp1 multigene family exert opposite transcriptional regulation of the long terminal repeat of HIV-1
    B Majello
    Dipartimento di Genetica, Biologia Generale e Molecolare, Universita Federico II, Naples, Italy
    Nucleic Acids Res 22:4914-21. 1994
    ..We found that the cooperative interaction between NF-kappa B and Sp1 is highly specific, since neither Sp3 nor Sp4 is capable of cooperating with NF-kappa B...
  59. pmc Sp1 acetylation is associated with loss of DNA binding at promoters associated with cell cycle arrest and cell death in a colon cell line
    Jennifer S Waby
    Department of Oncology, University of Sheffield, Medical School, Beech Hill Road, Sheffield, UK
    Mol Cancer 9:275. 2010
    ..Taken together our data suggest a mechanistic model for the chemopreventive actions of butyrate in colon epithelial cells, and provide new insight into the differential activities some classes of HDAC inhibitors...
  60. ncbi Betulinic acid inhibits prostate cancer growth through inhibition of specificity protein transcription factors
    Sudhakar Chintharlapalli
    Institute of Biosciences and Technology, Texas A and M University Health Science Center, Houston, Texas 77843 4466, USA
    Cancer Res 67:2816-23. 2007
    ..Thus, betulinic acid acts as a novel anticancer agent through targeted degradation of Sp proteins that are highly overexpressed in tumors...
  61. ncbi Promoter structure and transcription initiation sites of the human death receptor 5/TRAIL-R2 gene
    T Yoshida
    Department of Preventive Medicine, Kyoto Prefectural University of Medicine, Japan
    FEBS Lett 507:381-5. 2001
    ..Site-directed mutagenesis demonstrated that the DR5 gene promoter has no typical TATA-box, but has two Sp1 sites responsible for the basal transcription activity of the DR5 gene promoter...
  62. ncbi Oxidative stress regulates vascular endothelial growth factor-A gene transcription through Sp1- and Sp3-dependent activation of two proximal GC-rich promoter elements
    Georgia Schäfer
    Medizinische Klinik mit Schwerpunkt Hepatologie, Gastroenterologie, Endokrinologie und Stoffwechsel, Charite, Campus Virchow Klinikum, 13353 Berlin, Germany
    J Biol Chem 278:8190-8. 2003
    ..This study for the first time delineates the mechanisms underlying regulation of VEGF-A gene transcription by oxidative stress and thereby further elucidates potential pathways underlying redox control of neoangiogenesis...
  63. ncbi PMA induces the MUC5AC respiratory mucin in human bronchial epithelial cells, via PKC, EGF/TGF-alpha, Ras/Raf, MEK, ERK and Sp1-dependent mechanisms
    Christopher A Hewson
    Department of Respiratory Medicine, National Heart and Lung Institute, Imperial College London, Norfolk Place, London W2 1PG, UK
    J Mol Biol 344:683-95. 2004
    ..We have characterised a signalling pathway regulating PMA induction of MUC5AC. Studies such as this identify key signalling intermediates as targets for pharmacological intervention to treat mucus hypersecretion...
  64. ncbi Celecoxib inhibits vascular endothelial growth factor expression in and reduces angiogenesis and metastasis of human pancreatic cancer via suppression of Sp1 transcription factor activity
    Daoyan Wei
    Department of Gastrointestinal Medical Oncology, The University of Texas M D Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA
    Cancer Res 64:2030-8. 2004
    ..Collectively, our data provide a novel molecular mechanism for the antitumor activity of celecoxib and may help further improve its effectiveness in controlling pancreatic cancer growth and metastasis...
  65. ncbi Sp1 and Sp3 are oxidative stress-inducible, antideath transcription factors in cortical neurons
    Hoon Ryu
    Department of Neurology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Neurosci 23:3597-606. 2003
    ..Taken together, these results establish Sp1 and Sp3 as oxidative stress-induced transcription factors in cortical neurons that positively regulate neuronal survival...
  66. ncbi v-Src-mediated down-regulation of SSeCKS metastasis suppressor gene promoter by the recruitment of HDAC1 into a USF1-Sp1-Sp3 complex
    Yahao Bu
    Department of Cancer Genetics, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263, USA
    J Biol Chem 282:26725-39. 2007
    ..Our data suggest that suppression of the beta promoter is facilitated by Src-induced changes in the alpha promoter chromatinization mediated by a USF1-Sp1-Sp3 complex...
  67. ncbi Estrogen-occupied estrogen receptor represses cyclin G2 gene expression and recruits a repressor complex at the cyclin G2 promoter
    Fabio Stossi
    Department of Molecular and Integrative Physiology, Department of Chemistry, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    J Biol Chem 281:16272-8. 2006
  68. ncbi Molecular cloning and functional analysis of Drosophila TAF110 reveal properties expected of coactivators
    T Hoey
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720
    Cell 72:247-60. 1993
    ..These findings taken together suggest that TAF110 may function as a coactivator by serving as a site of protein-protein contact between activators like Sp1 and the TFIID complex...
  69. pmc Interactive roles of Ets-1, Sp1, and acetylated histones in the retinoic acid-dependent activation of guanylyl cyclase/atrial natriuretic peptide receptor-A gene transcription
    Prerna Kumar
    Department of Physiology, Tulane University Health Sciences Center and School of Medicine, New Orleans, Louisiana 70112, USA
    J Biol Chem 285:37521-30. 2010
    ..Collectively, the present results demonstrate that ATRA regulates Npr1 gene transcription and GC activity of the receptor by involving the interactive actions of Ets-1, Sp1, and histone acetylation...
  70. pmc Down-regulation of PROS1 gene expression by 17beta-estradiol via estrogen receptor alpha (ERalpha)-Sp1 interaction recruiting receptor-interacting protein 140 and the corepressor-HDAC3 complex
    Atsuo Suzuki
    Department of Pathophysiological Laboratory Sciences, Nagoya University Graduate School of Medicine, Nagoya 461 8673, Japan
    J Biol Chem 285:13444-53. 2010
  71. ncbi Crucial roles of Sp1 and epigenetic modifications in the regulation of the CLDN4 promoter in ovarian cancer cells
    Hiroshi Honda
    Laboratory of Cellular and Molecular Biology, NIA, National Institutes of Health, Baltimore, Maryland 21224, USA
    J Biol Chem 281:21433-44. 2006
  72. ncbi Sp1 and krüppel-like factor family of transcription factors in cell growth regulation and cancer
    A R Black
    Department of Pharmacology and Therapeutics, Roswell Park Cancer Institute, Buffalo, New York 14263, USA
    J Cell Physiol 188:143-60. 2001
    ..Thus, it is likely that the properties of the overall network of Sp/KLF factors play a determining role in regulation of cell growth and tumor progression...
  73. pmc A conserved alpha-helical motif mediates the interaction of Sp1-like transcriptional repressors with the corepressor mSin3A
    J S Zhang
    Gastroenterology Research Unit, Mayo Clinic, Rochester, Minnesota 55901, USA
    Mol Cell Biol 21:5041-9. 2001
  74. ncbi Up-regulation of DNA-dependent protein kinase activity and Sp1 in colorectal cancer
    Yoshio Hosoi
    Department of Radiation Research, Faculty of Medicine, University of Tokyo, Tokyo 113 0033, Japan
    Int J Oncol 25:461-8. 2004
    ..These results suggest that DNA-PK activity and protein- and mRNA-levels of Ku70, Ku80 and DNA-PKcs were elevated in tumor tissues in patients with colorectal cancer because of elevated Sp1 protein levels in tumor tissues...
  75. pmc HIF-2alpha promotes hypoxic cell proliferation by enhancing c-myc transcriptional activity
    John D Gordan
    Abramson Family Cancer Research Institute, School of Medicine, University of Pennsylvania, 421 Curie Boulevard, Philadelphia, PA 19104, USA
    Cancer Cell 11:335-47. 2007
    ..Enhanced c-Myc activity likely contributes to HIF-2alpha-mediated neoplastic progression following loss of the VHL tumor suppressor and influences the behavior of hypoxic tumor cells...
  76. ncbi Identification of a critical Sp1 site within the endoglin promoter and its involvement in the transforming growth factor-beta stimulation
    L M Botella
    Centro de Investigaciones Biologicas, Consejo Superior de Investigaciones Cientificas, Velazquez 144, 28006 Madrid, Spain
    J Biol Chem 276:34486-94. 2001
    ..The molecular mechanism underlying this cooperation appears to involve a direct physical interaction between Sp1 and Smad3/Smad4...
  77. pmc Central role of Sp1-regulated CD39 in hypoxia/ischemia protection
    Holger K Eltzschig
    Department of Anesthesiology and Perioperative Medicine, University of Colorado Health Sciences Center, Denver, CO, USA
    Blood 113:224-32. 2009
    ..In summary, these results identify a novel Sp1-dependent regulatory pathway for CD39 and indicate the likelihood that CD39 is central to protective responses to hypoxia/ischemia...
  78. pmc Paraoxonase-1 promoter haplotypes and serum paraoxonase: a predominant role for polymorphic position - 107, implicating the Sp1 transcription factor
    Sara Deakin
    Clinical Diabetes Unit, Division of Endocrinology and Diabetology, Medical Faculty, University Hospital, 1211 Geneva 14, Switzerland
    Biochem J 372:643-9. 2003
    ..A potential role for the transcription factor Sp1 has been demonstrated, which is consistent with the disruption of an Sp1 recognition sequence by the -107 polymorphism...
  79. ncbi Sp1/Sp3 compound heterozygous mice are not viable: impaired erythropoiesis and severe placental defects
    Imme Krüger
    Institut fur Molekularbiologie und Tumorforschung, Philipps Universitat Marburg, Marburg, Germany
    Dev Dyn 236:2235-44. 2007
    ..Our findings demonstrate that a threshold of Sp1 and Sp3 activity is required for normal embryonic development, suggesting that Sp1 and Sp3 act cooperatively to regulate downstream targets...
  80. ncbi Reduced bone density and osteoporosis associated with a polymorphic Sp1 binding site in the collagen type I alpha 1 gene
    S F Grant
    Department of Medicine and Therapeutics, University of Aberdeen, UK
    Nat Genet 14:203-5. 1996
  81. ncbi Neuronal injury-inducible gene is synergistically regulated by ATF3, c-Jun, and STAT3 through the interaction with Sp1 in damaged neurons
    Sumiko Kiryu-Seo
    Department of Anatomy and Neurobiology, Osaka City University, Graduate School of Medicine, 1 4 3 Asahimachi, Abeno Ku, Osaka 545 8585, Japan
    J Biol Chem 283:6988-96. 2008
    ..These findings suggested that Sp1 recruit ATF3, c-Jun, and STAT3 to obtain the requisite synergistic effect. Of these transcription factors, ATF3 may be the most critical, because ATF3 is specifically expressed after nerve injury...
  82. ncbi Regulation of telomerase activity by the p53 family member p73
    M Beitzinger
    Molecular Tumor Biology Group, Rudolf Virchow Center DFG Research Center for Experimental Biomedicine, University of Wurzburg, Versbacher Strasse 9, Würzburg 97078, Germany
    Oncogene 25:813-26. 2006
    ..These data provide evidence that the p73 gene functions as an important regulator of telomerase activity with implications for embryonic development, cellular differentiation and tumorigenesis...
  83. pmc Transcription factor E2F-associated phosphoprotein (EAPP), RAM2/CDCA7L/JPO2 (R1), and simian virus 40 promoter factor 1 (Sp1) cooperatively regulate glucocorticoid activation of monoamine oxidase B
    Kevin Chen
    Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, 1985 Zonal Avenue, PSC 518, Los Angeles, CA 90089, USA
    Mol Pharmacol 79:308-17. 2011
    ..Together, this study uncovers for the first time the molecular mechanisms for glucocorticoid activation of MAO B gene and provides new insights into the hormonal regulation of MAO...
  84. ncbi Sp1 is a co-activator with Ets-1, and Net is an important repressor of the transcription of CTP:phosphocholine cytidylyltransferase alpha
    Hiroyuki Sugimoto
    Department of Molecular Biochemistry, Gunma University Graduate School of Medicine, Maebashi 371 8511, Japan
    J Biol Chem 280:40857-66. 2005
    ..These results indicated that Net is an important endogenous repressor for CTalpha transcription...
  85. ncbi Promoter function and the role of cytokines in the transcriptional regulation of rabbit CYP2E1 and CYP2E2
    H M Peng
    Department of Biological Chemistry, Medical School, The University of Michigan, Ann Arbor 48109, USA
    Arch Biochem Biophys 382:129-37. 2000
    ..Increased DNA binding and Sp1 protein content as a result of IL-1alpha treatment, as well as cotransfection experiments with pPacSp1, suggest that Sp1 is a transcription activator for the induction of 2E1 by IL-1alpha in HepG2 cells...
  86. ncbi 2-Tellurium-bridged β-cyclodextrin, a thioredoxin reductase inhibitor, sensitizes human breast cancer cells to TRAIL-induced apoptosis through DR5 induction and NF-κB suppression
    Tingting Lin
    State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, People s Republic of China
    Carcinogenesis 32:154-67. 2011
    ..Overall, 2-TeCD sensitizes resistant breast cancer cells to TRAIL-based apoptosis in vitro and in vivo. These findings provide strong evidence for the therapeutic potential of this combination against breast cancers...
  87. ncbi REST is a key regulator in brain-specific homeobox gene expression during neuronal differentiation
    So Yun Park
    Department of Biological Sciences, Korea Advanced Institute of Science and Technology KAIST, Daejeon, Korea
    J Neurochem 103:2565-74. 2007
    ..The CpG sites of NRSE and GC box were completely unmethylated, signifying no interference of DNA methylation. Our results suggest that binding of REST to NRSE suppresses the Sp1-mediated activation of Bsx in non-neuronal cells...
  88. pmc Transcriptional control of KCNQ channel genes and the regulation of neuronal excitability
    Mariusz Mucha
    Institute of Membrane and Systems Biology, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK
    J Neurosci 30:13235-45. 2010
    ..We propose that these mechanisms are important in the control of excitability of neurons and may have implications in seizure activity and pain...
  89. ncbi p300 collaborates with Sp1 and Sp3 in p21(waf1/cip1) promoter activation induced by histone deacetylase inhibitor
    H Xiao
    Department of Basic Gerontology, National Institute for Longevity Sciences, 36 3, Gengo Morioka cho, Obu, Aichi, 474 8522 Japan
    J Biol Chem 275:1371-6. 2000
    ..Our data also suggested that p300 collaborates with Sp1 in a way which is different from that when p300 collaborates with p53 in p21 transcription...
  90. pmc Arsenic suppresses gene expression in promyelocytic leukemia cells partly through Sp1 oxidation
    Wen Chien Chou
    Department of Laboratory Medicine, National Taiwan University Hospital
    Blood 106:304-10. 2005
    ..We further explored the mechanisms by which ROS affects gene regulation and found that the Sp1 transcription factor was oxidized by arsenic treatment, with a corresponding decrease in its in situ binding on the promoters ..
  91. ncbi Physical interaction between retinoic acid receptor and Sp1: mechanism for induction of urokinase by retinoic acid
    Y Suzuki
    Laboratory of Molecular Cell Sciences, Tsukuba Life Science Center, The Institute of Physical and Chemical Research RIKEN, Tsukuba, Ibaraki, Japan
    Blood 93:4264-76. 1999
    ..These results suggest that (1) RA induces RARs mainly via RARalpha and that (2) RAR/RXR physically and functionally interact with Sp1, resulting in a potentiation of uPA transcription...
  92. pmc Aberrant regulation of HDAC2 mediates proliferation of hepatocellular carcinoma cells by deregulating expression of G1/S cell cycle proteins
    Ji Heon Noh
    Department of Pathology, College of Medicine and Functional RNomics Research Center, The Catholic University of Korea, Seoul, Korea
    PLoS ONE 6:e28103. 2011
  93. ncbi Induction of VEGF gene expression by retinoic acid through Sp1-binding sites in retinoblastoma Y79 cells
    Hideo Akiyama
    Department of Ophthalmology, Gunma University School of Medicine, 3 39 15 Showa Machi, Maebashi, Gunma 371 8511, Japan
    Invest Ophthalmol Vis Sci 43:1367-74. 2002
    ..In this study, we investigated the effects of atRA on expression of the VEGF gene in retinoblastoma Y79 cells...
  94. ncbi POZ domain transcription factor, FBI-1, represses transcription of ADH5/FDH by interacting with the zinc finger and interfering with DNA binding activity of Sp1
    Dong Kee Lee
    Department of Biochemistry and Molecular Biology, BK21 Project for Medical Sciences, Institute of Genetic Sciences, Yonsei University School of Medicine, 134 Shinchon dong, Seodaemoon ku, Seoul 120 752, Korea
    J Biol Chem 277:26761-8. 2002
    ..Our data suggest that POZ domain represses transcription by interacting with Sp1 zinc fingers and by interfering with the DNA binding activity of Sp1...
  95. ncbi Histone deacetylases are critical targets of bortezomib-induced cytotoxicity in multiple myeloma
    Jiro Kikuchi
    Division of Stem Cell Regulation, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan
    Blood 116:406-17. 2010
    ..These results suggest that bortezomib targets HDACs via distinct mechanisms from conventional HDAC inhibitors. Our findings provide a novel molecular basis and rationale for the use of bortezomib in MM treatment...
  96. ncbi Molecular cloning and characterization of TIEG2 reveals a new subfamily of transforming growth factor-beta-inducible Sp1-like zinc finger-encoding genes involved in the regulation of cell growth
    T Cook
    Gastroenterology Research Unit, Saint Marys Hospital, Mayo Clinic, Rochester, Minnesota 55905, USA
    J Biol Chem 273:25929-36. 1998
    ..Thus, TIEG2, together with TIEG1, defines a new subfamily of TGF-beta-inducible Sp1-like proteins involved in the regulation of cell growth...
  97. pmc Regulation of the ClC-2 lung epithelial chloride channel by glycosylation of SP1
    Neeraj Vij
    Eudowood Division of Pediatric Respiratory Sciences, Department of Pediatrics, The Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA
    Am J Respir Cell Mol Biol 34:754-9. 2006
    ..A high level of luminal ClC-2 protein expression is maintained by the SP1 transcription factor until SP1 and ClC-2 decline rapidly at birth...
  98. pmc Progesterone induces expression of the prolactin receptor gene through cooperative action of Sp1 and C/EBP
    Anita S Goldhar
    Mammary Biology and Tumorigenesis Laboratory, Center for Cancer Research, NCI, Bethesda, MD 20892 4254, USA
    Mol Cell Endocrinol 335:148-57. 2011
  99. ncbi Sp1 increases expression of cyclooxygenase-2 in hypoxic vascular endothelium. Implications for the mechanisms of aortic aneurysm and heart failure
    Q Xu
    Section on Cardiology, Department of Medicine, and Department of Physiology and Pharmacology, Wake Forest University School of Medicine, Winston Salem, North Carolina 27157 1045, USA
    J Biol Chem 275:24583-9. 2000
    ..The regulatory role of Sp1 discovered in this work supports the concept that a mechanistic link exists between vascular cellular hypoxia and mediators of inflammation associated with aortic aneurysm and heart failure...
  100. ncbi ZEB2 upregulates integrin α5 expression through cooperation with Sp1 to induce invasion during epithelial-mesenchymal transition of human cancer cells
    Eun Hee Nam
    Therapeutic Antibody Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak ro, Yuseong Gu, Daejon 305 806, Korea
    Carcinogenesis 33:563-71. 2012
    ..These findings increase our understanding of the pathways beyond E-cadherin reduction that regulate mesenchymal gene expression during EMT and cancer progression...
  101. pmc Repression of the luteinizing hormone receptor gene promoter by cross talk among EAR3/COUP-TFI, Sp1/Sp3, and TFIIB
    Ying Zhang
    Section on Molecular Endocrinology, Endocrinology, and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Cell Biol 23:6958-72. 2003

Research Grants63

  1. Enhance Production of Functional Recombinant Human Protein C Variant In Mammalian
    Nienwen Chow; Fiscal Year: 2010
    ..or SOD1G37R mutants via protease activated receptors 1 and 3, which inhibits nuclear transport of the SP1 transcription factor. In SOD1G93A mice, 5A-APC eliminated microhemorrhages in the spinal cord and BSCB leakage and reduced ..
  2. Role of TWEAK in Skeletal Muscle Metabolism
    Ashok Kumar; Fiscal Year: 2013
    ..Hypothesis 3: Demethylation of CpG islands in Fn14 promoter followed by binding of SP1 transcription factor triggers the expression of Fn14 in skeletal muscle in catabolic states...
  3. Transcriptional HIV-1 latency in astrocyte and macrophage reservoirs of the centr
    Melissa Churchill; Fiscal Year: 2013
    ..blood mononuclear cells (PBMC) of the same subjects, and are defined by alterations associated with the Sp1 transcription factor binding motif...
  4. Regional Control of Telencephalic Neuronal Diversity
    Kenneth Campbell; Fiscal Year: 2009
    ..We have recently identified a new marker of the dLGE called Sp8, which is a new member of the Sp1 transcription factor family...
    SUNANDITA BANERJI; Fiscal Year: 2003
    ..the MCF-7 late passage cells is repressed at the level of transcription due to inadequate levels of the Sp1 transcription factor. Therefore, these cells represent an ideal model system for examining a non-mutational mechanism of re-..
  6. 2,3,7,8,--TCDD Effects on the Estrogenic Responses
    Stephen Safe; Fiscal Year: 2006
    ..The proposed Aims will address important mechanistic considerations on IDRE-independent inhibitory AhR-ERa crosstalk and pathways critical for inhibition of mammary tumor growth by SAhRMs. ..
  7. Mechanisms of Growth Factor-Estrogen Receptor Crosstalk
    Stephen Safe; Fiscal Year: 2006
    ..abstract_text> ..
    Stephen Safe; Fiscal Year: 2002
    ..These results will ultimately facilitate design of chemotherapeutic approaches which target specific genes. ..
  9. Colon Cancer Inhibition by a Class of PPARgamma Agonists
    Stephen Safe; Fiscal Year: 2009
    ..The proposed studies will provide mechanistic insights on PPARy-dependent inhibition of colon cancer and identify compounds for future clinical studies. ..
  10. Coactivation of Estrogen Receptor alpha/Sp1
    Stephen Safe; Fiscal Year: 2006
    ..abstract_text> ..
    Stephen Safe; Fiscal Year: 2007
    ..The proposed training program will continue to provide trainees with a challenging academic and research environment that will prepare them for future careers in academic, industry and government. ..
    Stephen Safe; Fiscal Year: 2001
    ..Thus the proposed studies will define a new mechanism-based class of antiestrogens that can be used alone or in combination with tamoxifen for treatment of breast and endometrial cancer. ..
  13. P21 transcriptional inhibitors as proapoptotic compounds
    Andrei Gartel; Fiscal Year: 2003
    ..This study may improve treatment of cancer by leading to the identification of new compounds that will increase the efficiency of cell death promoting anticancer drugs for cancer treatment. ..
  14. Inhibition of Pancreatic Cancer by New PPARgamma Agonis*
    Stephen Safe; Fiscal Year: 2008
    ..PPAR? -active C-substituted DIMs that inhibit pancreatic cancer cell growth in vitro and in vivo, and these will serve as lead compounds for development of new drugs for clinical treatment of this devastating disease ..
  15. Regulation of SMAD2 Signaling by SMP1 Phosphatase
    Xia Lin; Fiscal Year: 2008
    ..The results will be pertinent towards development for a foundation for the rational design of novel therapeutic approaches for prevention and treatment of human cancers and other diseases. ..
  16. Genomic Instability and Evolution of Drug Resistance
    MACUS KUO; Fiscal Year: 2008
    ..We anticipate from these studies to gain important insights into the function of FSA in the regulation of cell growth and differentiation in normal and malignant epithelial cells. ..
    Richard Tsika; Fiscal Year: 2008
    ..abstract_text> ..
  18. 15-Lipoxygenase 2 is a Prostate Senescence Gene
    DEAN TANG; Fiscal Year: 2008
    ..Our long-term goal is to utilize the data obtained from this project to develop 15-LOX-2-targeted anti-PCa therapeutics that trigger cell-cycle arrest and senescence. ..
  19. Jade-1 in cystic renal disease
    Herbert Cohen; Fiscal Year: 2008
    ..abstract_text> ..
    Juanita Merchant; Fiscal Year: 2007
    ..For type specifications, see instructions on page 6.) RESEARCH GRANT TABLE OF CONTENTS Page Numbers Face Page 1 Description, ..
  21. Genotypic Determinants of Aspirin Response in High Risk Families
    Lewis Becker; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  22. A Novel Mechanism to Regulate Caspase-8 Activation on the Mitochondrial Membrane
    DEAN TANG; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  23. Proteasome regulation by O-glycosylation
    JEFFREY KUDLOW; Fiscal Year: 2007
    ..4. Using transgenic mice, determine the effect of proteasome blockade in vivo on epithelial cell apoptosis and muscle protein wasting. ..
  24. Peroxiredoxin 6 and Cataractogenesis
    Dhirendra P Singh; Fiscal Year: 2010
    ..The proposed studies will provide a sound scientific basis for developing an antioxidant-based therapy or combination of therapies for preventing cataractogenesis and age-associated degenerative diseases in general. ..
  25. Muscle-Specific Nutritional Adaptations to Catabolic States
    S Russ Price; Fiscal Year: 2010
    ..New information regarding the mechanisms of muscle atrophy may identify therapeutic targets to reduce morbidity and mortality of chronically ill patients and improve their quality of life. ..
  26. Molecular Basis of mu-Opioid Receptor Gene Regulation
    Jane Ko; Fiscal Year: 2009
    ..abstract_text> ..
    GIANFRANCO D ALPINI; Fiscal Year: 2010
    ..It is our firm belief that the results of these studies will provide information that will not only allow us to better understand cholangiocyte pathobiology but will also lead to treatments for cholestatic liver diseases. ..
  28. PSF Regulates the Porcine P450scc IFGRE
    Randall J Urban; Fiscal Year: 2010
    ..This knowledge will increase our understanding and provide the basic background for novel concepts on hormonal control of ovarian steroidogenesis. ..
    BRUCE KONE; Fiscal Year: 2002
  30. Molecular Mechanism of Protein Kinase Cbeta-Mediated Cholesterol Homeostasis
    Kamal D Mehta; Fiscal Year: 2010
    ..The greatest potential for arresting this epidemic is likely to come through a clearer understanding of its molecular pathogenesis to develop rational and mechanism based therapy. ..
  31. Lipid Raft Microdomains in Neutrophil Function
    ROBERT GARY SITRIN; Fiscal Year: 2010
    ..Hopefully, targeting the function of lipid raft microdomains will engender new therapeutic strategies for enhancing antimicrobial defenses and suppressing the deleterious effects of acute inflammation. ..
  32. Redox Regulation of Multidrug Resistance Gene Expression
    MACUS KUO; Fiscal Year: 2009
    ..We hope from these studies to learn the molecular bases of redox conditions that affect drug sensitivity through the regulation of their respective drug resistance gene expression. ..
  33. Genome-Wide Association of Platelet Phenotypes
    Lewis Becker; Fiscal Year: 2009
    ..This study represents the first genome -wide SNP association study of comprehensive platelet function and should lead to novel tailored anti-platelet therapy for the prevention of vascular thromboses. (End of Abstract) ..
  34. Development of Novel Regimens for the Prevention of Chronic Renal Failure
    CARL SHELLEY; Fiscal Year: 2008
    ..In this way we expect to provide proof-of-principal for new therapeutic regimens that reduce the ability of hypoxia to cause CRF. ..
    ROSALIA SIMMEN; Fiscal Year: 2008
    ..This research will provide novel mechanistic insights into PR signaling involving PR coregulators that have relevance to human subfertility and hormone-dependent gynecological diseases. ..
  36. Combination Drug Therapy in Huntington's Disease
    ROBERT FERRANTE; Fiscal Year: 2008
    ..abstract_text> ..
  37. Molecular Mechanisms of Global Represion in Yeast
    Kevin Struhl; Fiscal Year: 2008
    ..We will use RNAi to identify genes affected by the various TLE proteins, and will address the mechanism of repression by TLE proteins by using derivatives of the well characterized B-interferon promoter. ..
    MACUS KUO; Fiscal Year: 2004
    ..We hope from this research, which includes in vitro cell culture and in vivo animal and tumor models, to gain important molecular insights into the genetic resolution of drug resistance and hepatocarcinogenesis. ..
  39. Mechanisms of p42/44MAPK-induced LDL receptor expression
    KAMAL MEHTA; Fiscal Year: 2004
    ..This knowledge could be exploited to develop improved hypercholesterolemia therapies and to reduce foam cell formation. ..
    DEAN TANG; Fiscal Year: 2004
    ..abstract_text> ..
  41. Novel Function of PI 3-Kinase in Calcium Regulation
    Ching Shih Chen; Fiscal Year: 2004
    ..These studies will help understand how the PI(3,4,5)P3-binding protein mediates Ca2+ entry across the plasma membrane. ..
  42. New DHFR Deletion Polymorphism - A Risk Factor for SB?
    William Johnson; Fiscal Year: 2003
    ..Forms of foliate that are already reduced could be preferable as foliate supplements during pregnancy for prevention of SB. ..
    Christie Thomas; Fiscal Year: 2003
    ..The long-term objectives are to understand the regulation and function of ENaC in transepithelial Na+ transport. ..
    SEKHAR POTHIREDDY; Fiscal Year: 2002
    ..This study will help us better understand the plasticity of airway epithelial cell differentiation and the response of airway epithelial cells to environmental insults. ..
  45. Regulation of Intestinal Nutrient Transporters
    Martin Martin; Fiscal Year: 2002
    ..The identification and further characterization of these cis elements will provide better insight into the molecular mechanism of enterocyte-specific cell fate and the various factors mediating its regulation. ..
    Martin Martin; Fiscal Year: 2002
    ..Analysis of the FcRn and pIgR genes offers a unique model that may lead to a better understanding of the molecular mechanism of intestinal ontogeny, and specifically the intrinsic clock which controls its regulation. ..
  47. Testing of Novel Apoptotic Agents in Prostate Cancer
    Ching Shih Chen; Fiscal Year: 2002
    ..Together, these animal-- model studies will validate that the in vitro apoptosis-inducing effect of these compounds is relevant for tumor growth inhibition in vivo. ..
  48. Apoptosis Regulation by Lipid Signals in Prostate Cancer
    Ching Shih Chen; Fiscal Year: 2005
    ..Identification of new anti-cancer agents whose mode of action is distinct from existing chemotherapeutic regimens will foster new therapeutic strategies. ..
  49. Novel Celecoxib Derivatives as Chemopreventive Agents
    Ching Shih Chen; Fiscal Year: 2005
    ..In parallel, we will examine the safety of chronic oral treatment with these compounds in non-carcinogen-treated tumor-free animals. ..
  50. Sgk1 in Na+ transport in fetal lung epithelia
    Christie Thomas; Fiscal Year: 2007
    ..abstract_text> ..
  51. Reactive astrocytes from gliotic capsule
    J Marc Simard; Fiscal Year: 2007
    ..unreadable] [unreadable]..
    J Marc Simard; Fiscal Year: 2007
    ..abstract_text> ..
  53. Altering Gastric Epithelial Cell Differentiation
    Juanita Merchant; Fiscal Year: 2007
    ..These studies will further our understanding of how corpus atrophy predisposes the gastric mucosa to neoplastic transformation. [unreadable] [unreadable]..
  54. Structure/Function of Varicella Zoster Virus DNA
    William Ruyechan; Fiscal Year: 2007
    ..We will also examine the recently identified interaction between IE63 and the host RNA Polymerase I1. ..
  55. Coronary Disease Detection by Thalluim SPECT and Fast CT
    Lewis Becker; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  56. Mechanisms of Redox Mediated Cardioprotection
    Daret St Clair; Fiscal Year: 2006
    ..The results could lead to the development of selective approaches to improve protection of the heart, thus reducing the toxicity of free radical-associated anti-cancer agents. ..
  57. Control of SnoN Activity by SUMO modification
    Xia Lin; Fiscal Year: 2006
    ..Finally, the results may also provide a foundation for the rational design of novel therapeutic approaches for prevention and treatment of cancer and musculoskeletal diseases. [unreadable] [unreadable]..
  58. Targeted Prodrug Therapy of Liver Cancers
    MACUS KUO; Fiscal Year: 2005
    Lewis Becker; Fiscal Year: 2001
    ..This study will provide important insights into the phenotypic and genetic determinants of clinical and occult CHD in families with premature CHD. ..
    J Simard; Fiscal Year: 2004
  61. Intestinal Iron Transport in Iron Deficiency/Anemia
    James Collins; Fiscal Year: 2005
    ..abstract_text> ..
    Michael Coffey; Fiscal Year: 2002
    ..This proposal will further enhance our ability to correct this defect and help boost AM, PBM, and PMN defenses against opportunistic infections in the lung in HIV disease. ..