dental enamel proteins

Summary

Summary: The proteins that are part of the dental enamel matrix.

Top Publications

  1. pmc Ameloblastin is a cell adhesion molecule required for maintaining the differentiation state of ameloblasts
    Satoshi Fukumoto
    Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 167:973-83. 2004
  2. ncbi A nonsense mutation in the enamelin gene causes local hypoplastic autosomal dominant amelogenesis imperfecta (AIH2)
    Carina K Mårdh
    Department of Medical Genetics Medical Biosciences, Umea University, S 901 87 Umea, Sweden
    Hum Mol Genet 11:1069-74. 2002
  3. ncbi Autosomal-dominant hypoplastic form of amelogenesis imperfecta caused by an enamelin gene mutation at the exon-intron boundary
    M Kida
    Research Group of Human Gene Therapy, Hokkaido University Graduate School of Medicine, N 15, W 7, Kita ku, Sapporo, 060 8638, Hokkaido, Japan
    J Dent Res 81:738-42. 2002
  4. ncbi Mutation of the gene encoding the enamel-specific protein, enamelin, causes autosomal-dominant amelogenesis imperfecta
    M H Rajpar
    School of Biological Sciences and Department of Dental Medicine and Surgery, 3 239 Stopford Building, University of Manchester, Oxford Road, Manchester M13 9PT, UK
    Hum Mol Genet 10:1673-7. 2001
  5. pmc Enamel defects and ameloblast-specific expression in Enam knock-out/lacz knock-in mice
    Jan C C Hu
    Department of Orthodontics and Pediatric Dentistry and Department of Biologic and Materials Sciences, University of Michigan School of Dentistry, Ann Arbor, Michigan 48108, USA
    J Biol Chem 283:10858-71. 2008
  6. pmc Critical role of heparin binding domains of ameloblastin for dental epithelium cell adhesion and ameloblastoma proliferation
    Akira Sonoda
    Section of Pediatric Dentistry, Division of Oral Health, Growth and Development, Kyushu University, Fukuoka 812 8582, Japan
    J Biol Chem 284:27176-84. 2009
  7. pmc Target gene analyses of 39 amelogenesis imperfecta kindreds
    Hui Chen Chan
    Department of Biologic and Materials Sciences, University of Michigan School of Dentistry, Ann Arbor, MI 48108, USA
    Eur J Oral Sci 119:311-23. 2011
  8. ncbi The effect of Emdogain on the growth and differentiation of rat bone marrow cells
    J van den Dolder
    Department of Periodontology and Biomaterials, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands
    J Periodontal Res 41:471-6. 2006
  9. doi Novel FAM20A mutations in hypoplastic amelogenesis imperfecta
    Sang Hyun Cho
    Department of Pediatric Dentistry and Dental Research Institute, School of Dentistry, Seoul National University, Chongno Gu, Seoul, Korea
    Hum Mutat 33:91-4. 2012
  10. ncbi Amelogenin p.M1T and p.W4S mutations underlying hypoplastic X-linked amelogenesis imperfecta
    J W Kim
    Department of Orthodontics and Pediatric Dentistry, University of Michigan Dental Research Lab, 1210 Eisenhower Place, Ann Arbor, MI 48108, USA
    J Dent Res 83:378-83. 2004

Detail Information

Publications239 found, 100 shown here

  1. pmc Ameloblastin is a cell adhesion molecule required for maintaining the differentiation state of ameloblasts
    Satoshi Fukumoto
    Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 167:973-83. 2004
    ..Thus, ameloblastin is a cell adhesion molecule essential for amelogenesis, and it plays a role in maintaining the differentiation state of secretory stage ameloblasts by binding to ameloblasts and inhibiting proliferation...
  2. ncbi A nonsense mutation in the enamelin gene causes local hypoplastic autosomal dominant amelogenesis imperfecta (AIH2)
    Carina K Mårdh
    Department of Medical Genetics Medical Biosciences, Umea University, S 901 87 Umea, Sweden
    Hum Mol Genet 11:1069-74. 2002
    ..These findings support ENAM as a disease gene, and shed new light on the molecular mechanism of the disease and to the function of the enamelin protein in enamel formation...
  3. ncbi Autosomal-dominant hypoplastic form of amelogenesis imperfecta caused by an enamelin gene mutation at the exon-intron boundary
    M Kida
    Research Group of Human Gene Therapy, Hokkaido University Graduate School of Medicine, N 15, W 7, Kita ku, Sapporo, 060 8638, Hokkaido, Japan
    J Dent Res 81:738-42. 2002
    ..Thus, heterogeneous mutations in the enamelin gene are responsible for an autosomal-dominant hypoplastic form of AI...
  4. ncbi Mutation of the gene encoding the enamel-specific protein, enamelin, causes autosomal-dominant amelogenesis imperfecta
    M H Rajpar
    School of Biological Sciences and Department of Dental Medicine and Surgery, 3 239 Stopford Building, University of Manchester, Oxford Road, Manchester M13 9PT, UK
    Hum Mol Genet 10:1673-7. 2001
    ..As this type of AI is clinically distinct from that localized previously to chromosome 4q11-q21, these findings highlight the need for a molecular classification of this group of disorders...
  5. pmc Enamel defects and ameloblast-specific expression in Enam knock-out/lacz knock-in mice
    Jan C C Hu
    Department of Orthodontics and Pediatric Dentistry and Department of Biologic and Materials Sciences, University of Michigan School of Dentistry, Ann Arbor, Michigan 48108, USA
    J Biol Chem 283:10858-71. 2008
    ..These results demonstrate ameloblast-specific expression of enamelin and reveal that enamelin is essential for proper enamel matrix organization and mineralization...
  6. pmc Critical role of heparin binding domains of ameloblastin for dental epithelium cell adhesion and ameloblastoma proliferation
    Akira Sonoda
    Section of Pediatric Dentistry, Division of Oral Health, Growth and Development, Kyushu University, Fukuoka 812 8582, Japan
    J Biol Chem 284:27176-84. 2009
    ....
  7. pmc Target gene analyses of 39 amelogenesis imperfecta kindreds
    Hui Chen Chan
    Department of Biologic and Materials Sciences, University of Michigan School of Dentistry, Ann Arbor, MI 48108, USA
    Eur J Oral Sci 119:311-23. 2011
    ..We conclude that mutation analyses of the current candidate genes for AI have about a 50% chance of identifying the disease-causing mutation in a given kindred...
  8. ncbi The effect of Emdogain on the growth and differentiation of rat bone marrow cells
    J van den Dolder
    Department of Periodontology and Biomaterials, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands
    J Periodontal Res 41:471-6. 2006
    ..Emdogain (EMD) consists of enamel matrix proteins which are known to stimulate bone formation. However, only a few studies in the literature have reported the effect of EMD on osteoblast-like cells in vitro...
  9. doi Novel FAM20A mutations in hypoplastic amelogenesis imperfecta
    Sang Hyun Cho
    Department of Pediatric Dentistry and Dental Research Institute, School of Dentistry, Seoul National University, Chongno Gu, Seoul, Korea
    Hum Mutat 33:91-4. 2012
    ..This study confirms the importance of the FAM20A protein in enamel biomineralization as well as tooth eruption...
  10. ncbi Amelogenin p.M1T and p.W4S mutations underlying hypoplastic X-linked amelogenesis imperfecta
    J W Kim
    Department of Orthodontics and Pediatric Dentistry, University of Michigan Dental Research Lab, 1210 Eisenhower Place, Ann Arbor, MI 48108, USA
    J Dent Res 83:378-83. 2004
    ..Dentin was normal. The severity of the enamel phenotype correlated with the predicted effects of the mutations on amelogenin expression and secretion...
  11. ncbi Identification of a nonsense mutation in the amelogenin gene (AMELX) in a family with X-linked amelogenesis imperfecta (AIH1)
    M J Aldred
    Institute of Medical Genetics, University of Wales College of Medicine, Heath Park, Cardiff, UK
    Hum Genet 90:413-6. 1992
    ..We propose that the amelogenin gene is implicated in both the formation of enamel of normal thickness and in the normal mineralisation process...
  12. ncbi Amelogenin-deficient mice display an amelogenesis imperfecta phenotype
    C W Gibson
    Department of Anatomy and Histology, University of Pennsylvania School of Dental Medicine, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 276:31871-5. 2001
    ..These null mice will be useful for understanding the functions of amelogenin proteins during enamel formation and for developing therapeutic approaches for treating this developmental defect that affects the enamel...
  13. pmc Enamel matrix proteins; old molecules for new applications
    S P Lyngstadaas
    Department of Biomaterials, Faculty of Dentistry, University of Oslo, Oslo, Norway
    Orthod Craniofac Res 12:243-53. 2009
    ..New experiments now confirm that amelogenins have potential for being used also in the fields of endodontics, bone regeneration, implantology, traumatology, and wound care...
  14. pmc The human enamel protein gene amelogenin is expressed from both the X and the Y chromosomes
    E C Salido
    Howard Hughes Medical Institute, University of California School of Medicine, Harbor UCLA Medical Center, Torrance
    Am J Hum Genet 50:303-16. 1992
    ....
  15. ncbi A deletion in the amelogenin gene (AMG) causes X-linked amelogenesis imperfecta (AIH1)
    M Lagerström
    Department of Medical Genetics, University of Uppsala, Sweden
    Genomics 10:971-5. 1991
    ..Our results link a defect in the amelogenin gene to the abnormal formation of enamel. We thus conclude that the amelogenin protein has a role in biomineralization of tooth enamel...
  16. ncbi Enamelin (Enam) is essential for amelogenesis: ENU-induced mouse mutants as models for different clinical subtypes of human amelogenesis imperfecta (AI)
    Hiroshi Masuya
    Mouse Functional Genomics Research Group, Tsukuba, Ibaraki, Japan
    Hum Mol Genet 14:575-83. 2005
    ..Thus, we report here that the Enam gene is essential for amelogenesis, and that mice with different point mutations at Enam may provide good animal models to study the different clinical subtypes of AI...
  17. ncbi Role of Hertwig's epithelial root sheath cells in tooth root development
    Margarita Zeichner-David
    Center for Craniofacial Molecular Biology, University of Southern California School of Dentistry, Los Angeles, California 90033, USA
    Dev Dyn 228:651-63. 2003
    ..These studies suggest that the acellular and cellular cementum are synthesized by two different types of cells, the first one by HERS-derived cementoblasts and the later by neural crest-derived cementoblasts...
  18. ncbi Imaging RNA polymerase-amelogenin gene complexes with single molecule resolution using atomic force microscopy
    Neal Crampton
    School of Physics and Astronomy, University of Leeds, and Department of Oral Biology, Leeds Dental Institute, UK
    Eur J Oral Sci 114:133-8; discussion 164-5, 380-1. 2006
    ....
  19. ncbi DNA sequence for cloned cDNA for murine amelogenin reveal the amino acid sequence for enamel-specific protein
    M L Snead
    Biochem Biophys Res Commun 129:812-8. 1985
    ..However, an additional 10-residues were found at the carboxy terminus of the murine amelogenin. This is the most complete sequence database for amelogenin peptides and the only DNA sequence for enamel specific genes...
  20. ncbi Identification of the leucine-rich amelogenin peptide (LRAP) as the translation product of an alternatively spliced transcript
    C W Gibson
    Department of Anatomy and Histology, School of Dental Medicine, University of Pennsylvania, Philadelphia 19104
    Biochem Biophys Res Commun 174:1306-12. 1991
    ..Analysis of the structure of LRAP suggests that the polypeptide might exhibit interesting properties relative to hydroxy apatite crystal formation...
  21. ncbi Ameloblastin fusion protein enhances pulpal healing and dentin formation in porcine teeth
    Y Nakamura
    Department of Clinical Cariology and Endodontology, School of Dentistry, Showa University, 2 1 1, Kitasenzoku, Ohta ku, Tokyo, 145 8515, Japan
    Calcif Tissue Int 78:278-84. 2006
    ..The results suggest potential for rAmbn as a biologically active pulp-dressing agent for enhanced pulpal wound healing and reparative dentin formation after pulpotomy procedures...
  22. doi Ameloblastin expression and putative autoregulation in mesenchymal cells suggest a role in early bone formation and repair
    Margareth V Tamburstuen
    Department of Biomaterials, Faculty of Dentistry, University of Oslo UiO, Oslo, Norway
    Bone 48:406-13. 2011
    ..Expression of ameloblastin was also detected in newly formed bone in experimental bone defects in adult rats. Together these findings suggest a role for this protein in early bone formation and repair...
  23. ncbi Effect of GBR in combination with deproteinized bovine bone mineral and/or enamel matrix proteins on the healing of critical-size defects
    Nikolaos Donos
    Department of Periodontology, Eastman Dental Institute, University College London, UK
    Clin Oral Implants Res 15:101-11. 2004
    ..To evaluate the effect of guided bone regeneration (GBR) in combination with or without deproteinized bovine bone mineral (DBBM) and/or an enamel matrix derivative (EMD) on the healing of critical-size calvarial defects...
  24. ncbi Amelogenin, a major structural protein in mineralizing enamel, is also expressed in soft tissues: brain and cells of the hematopoietic system
    Dan Deutsch
    Dental Research Laboratory, Institute of Dental Sciences, Hebrew University Hadassah, Faculty of Dental Medicine, Jerusalem, Israel, and Institute of Anatomy, Charite Medical Center, Berlin, Germany
    Eur J Oral Sci 114:183-9; discussion 201-2, 381. 2006
    ..Several different potential functions of amelogenin are discussed...
  25. pmc Enamel proteins and proteases in Mmp20 and Klk4 null and double-null mice
    Yasuo Yamakoshi
    Department of Biologic and Materials Sciences, University of Michigan School of Dentistry, Ann Arbor, MI 48108, USA
    Eur J Oral Sci 119:206-16. 2011
    ..These results support the importance of MMP20 during the secretory stage and of KLK4 during the maturation stage and show there is only limited functional redundancy for these enzymes...
  26. pmc Hydrolysis of amelogenin by matrix metalloprotease-20 accelerates mineralization in vitro
    Vuk Uskoković
    Division of Biomaterials and Bioengineering, Department of Preventive and Restorative Dental Sciences, University of California, Parnassus Avenue 707, San Francisco, CA 94143, USA
    Arch Oral Biol 56:1548-59. 2011
    ..6 mg/ml. Controlling the rate and the extent of the proteolytic cleavage can thus be used to control the nucleation and growth rates in a protein-guided crystallization system...
  27. doi Ameloblastin promotes bone growth by enhancing proliferation of progenitor cells and by stimulating immunoregulators
    Margareth V Tamburstuen
    Department of Biomaterials, Faculty of Dentistry, University of Oslo UiO, Oslo, Norway
    Eur J Oral Sci 118:451-9. 2010
    ..A receptor has not yet been identified, but we found rAmbn to induce, directly and indirectly, signal transducer and activator of transcription (STAT) 1 and 2 and downstream factors in the interferon pathway...
  28. doi The enamelin genes in lizard, crocodile, and frog and the pseudogene in the chicken provide new insights on enamelin evolution in tetrapods
    Nawfal Al-Hashimi
    Universite Pierre et Marie Curie, UMR 7138 Systématique Adaptation Evolution, Paris, France
    Mol Biol Evol 27:2078-94. 2010
    ....
  29. pmc Altered enamelin phosphorylation site causes amelogenesis imperfecta
    H C Chan
    Department of Biologic and Materials Sciences, University of Michigan School of Dentistry, 1011 N University, Ann Arbor, MI 48109 1078, USA
    J Dent Res 89:695-9. 2010
    ..Ser(216) is one of two serines on the 32-kDa enamelin that is phosphorylated by Golgi casein kinase and is thought to mediate calcium binding. We propose that phosphorylation of enamelin is critical for its function...
  30. doi Human cementoblasts express enamel-associated molecules in vitro and in vivo
    J Nunez
    Facultad de Odontologa, Universidad Complutense de Madrid, Espana
    J Periodontal Res 45:809-14. 2010
    ....
  31. pmc Regulation of dental enamel shape and hardness
    J P Simmer
    Department of Biologic and Materials Sciences, University of Michigan School of Dentistry, 1011 N University, Ann Arbor, MI 48109 1078, USA
    J Dent Res 89:1024-38. 2010
    ..Here we review the growth parameters that determine the shape of the enamel crown as well as the mechanisms of enamel appositional growth and maturation...
  32. pmc Ameloblastin regulates osteogenic differentiation by inhibiting Src kinase via cross talk between integrin beta1 and CD63
    Shinji Iizuka
    Department of Oral and Maxillofacial Pathobiology, Graduate School of Biomedical Sciences, Hiroshima University, Minami Ku, Hiroshima, Japan
    Mol Cell Biol 31:783-92. 2011
    ..Therefore, our results suggest that ameloblastin is expressed in osteoblasts and functions as a promoting factor for osteogenic differentiation via a novel pathway through the interaction between CD63 and integrin β1...
  33. doi Do bone grafts or barrier membranes provide additional treatment effects for infrabony lesions treated with enamel matrix derivatives? A network meta-analysis of randomized-controlled trials
    Yu Kang Tu
    Department of Periodontology, Leeds Dental Institute, Leeds, UK
    J Clin Periodontol 37:59-79. 2010
    ....
  34. ncbi Osteoprotegerin and receptor activator of nuclear factor-kappa B ligand modulation by enamel matrix derivative in human alveolar osteoblasts
    Carlo Galli
    Unit of Periodontology, University of Parma, Parma, Italy
    J Periodontol 77:1223-8. 2006
    ..Recent evidence suggests that EMD may contribute to inducing osteoblast growth and differentiation. We investigated the effects of EMD on growth and osteogenic marker modulation in human mandibular osteoblasts...
  35. ncbi Emdogain stimulates matrix degradation by osteoblasts
    S Goda
    Departments of Biochemistry, Osaka Dental University, Osaka, Japan
    J Dent Res 87:782-7. 2008
    ..These results suggest that Emdogain facilitates tissue regeneration through the activation of the collagenase, MMP-1, that degrades matrix proteins in bone tissue microenvironments...
  36. ncbi Sheathlin: cloning, cDNA/polypeptide sequences, and immunolocalization of porcine enamel sheath proteins
    C C Hu
    University of Texas Health Science Center at San Antonio, School of Dentistry, Department of Pediatric Dentistry 78284 7888, USA
    J Dent Res 76:648-57. 1997
    ..We propose that the porcine sheath proteins and their proteolytic cleavage products be designated "sheathlin"...
  37. doi Bioinformatic analysis and molecular modelling of human ameloblastin suggest a two-domain intrinsically unstructured calcium-binding protein
    Jirí Vymetal
    Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Flemingovo nAm, Prague, Czech Republic
    Eur J Oral Sci 116:124-34. 2008
    ..The model provides information useful for further studies on the function of AMBN...
  38. ncbi Comparative immunochemical analyses of the developmental expression and distribution of ameloblastin and amelogenin in rat incisors
    A Nanci
    Faculty of Dentistry, Universite de Montreal, Montreal, Quebec, Canada
    J Histochem Cytochem 46:911-34. 1998
    ..These data suggest a correlation between the distribution of amelogenin and ameloblastin and that intact ameloblastin has a transient role in promoting/stabilizing crystal elongation. (J Histochem Cytochem 46:911-934, 1998)..
  39. ncbi Enamel matrix derivative and bone healing after guided bone regeneration in dehiscence-type defects around implants. A histomorphometric study in dogs
    Marcio Z Casati
    Department of Prosthodontics and Periodontics, School of Dentistry at Piracicaba, UNICAMP, Sao Paulo, Brazil
    J Periodontol 73:789-96. 2002
    ..e., in the absence of periodontal ligament cells...
  40. ncbi Alternative splicing of the mouse amelogenin primary RNA transcript
    J P Simmer
    University of Texas School of Dentistry, Department of Pediatric Dentistry, San Antonio 78284 7888
    Calcif Tissue Int 55:302-10. 1994
    ..Antibodies were raised to synthetic exon 4-encoded polypeptides and used to immunostain Western transfers and histologic tooth sections...
  41. pmc Molecular decay of the tooth gene Enamelin (ENAM) mirrors the loss of enamel in the fossil record of placental mammals
    Robert W Meredith
    Department of Biology, University of California Riverside, Riverside, California, United States of America
    PLoS Genet 5:e1000634. 2009
    ..The molecular decay of ENAM parallels the morphological degeneration of enamel in the fossil record of placental mammals and provides manifest evidence for the predictive power of Darwin's theory...
  42. ncbi The effect of enamel matrix proteins and deproteinized bovine bone mineral on heterotopic bone formation
    Nikolaos Donos
    Eastman Dental Institute, UCL, Department of Periodontology, London, UK
    Clin Oral Implants Res 17:434-8. 2006
    ..Intramuscular bone formation was not encountered in any group. The implantation of DBBM particles alone, combined with EMD or its carrier (PGA) failed to exhibit extraskeletal, bone-inductive properties...
  43. pmc Construction and identification of mouse amelogenin cDNA clones
    M L Snead
    Proc Natl Acad Sci U S A 80:7254-8. 1983
    ..The mouse cDNA sequence hybridized to genomic mouse and human DNAs. This amelogenin cDNA probe now enables molecular investigations of a number of classical problems in developmental biology...
  44. ncbi Enamel matrix derivative (EMDOGAIN) rapidly stimulates phosphorylation of the MAP kinase family and nuclear accumulation of smad2 in both oral epithelial and fibroblastic human cells
    T Kawase
    Department of Pharmacology, Niigata University, Niigata, Japan
    J Periodontal Res 36:367-76. 2001
    ....
  45. ncbi Anti-TGF-beta antibody blocks enamel matrix derivative-induced upregulation of p21WAF1/cip1 and prevents its inhibition of human oral epithelial cell proliferation
    Tomoyuki Kawase
    Department of Pharmacology, Faculty of Dentistry, Niigata University, 2 5274 Gakkocho dori, Niigata 951 8514, Japan
    J Periodontal Res 37:255-62. 2002
    ....
  46. ncbi Effects of enamel matrix derivative on gene expression of primary osteoblasts
    J Jiang
    Department of Endodontology, School of Dental Medicine, University of Connecticut Health Center, Farmington, USA
    Oral Surg Oral Med Oral Pathol Oral Radiol Endod 91:95-100. 2001
    ..CONCLUSION: These results indicate that EMD might regulate certain gene expression during periodontal tissue regeneration...
  47. ncbi Porcine enamel matrix derivative enhances trabecular bone regeneration during wound healing of injured rat femur
    F Kawana
    Department of Oral Histology, School of Dentistry, Showa University, Shinagawa-ku, Tokyo, Japan
    Anat Rec 264:438-46. 2001
    ..Our results suggest that EMD possesses an osteo-promotive effect on bone and medullary regeneration during wound healing of injured long bones...
  48. ncbi Rare failures in the amelogenin sex test
    M Steinlechner
    Institute of Legal Medicine, Innsbruck, Austria
    Int J Legal Med 116:117-20. 2002
    ..The amplification of a fragment of the SRY gene gave positive results in all six samples, confirming the male phenotype of the individuals. The observed failure rate of the amelogenin sex test was 0.018% in this study...
  49. ncbi Amelogenins: assembly, processing and control of crystal morphology
    J Moradian-Oldak
    Center for Craniofacial Molecular Biology, University of Southern California School of Dentistry, 2250 Alcazar St, Los Angeles, CA 90033, USA
    Matrix Biol 20:293-305. 2001
    ..This paper summarizes current knowledge on the assembly, processing and effect on crystal morphology by amelogenin proteins. The correlation between these three events and putative functional roles for amelogenin protein are discussed...
  50. pmc Novel ENAM mutation responsible for autosomal recessive amelogenesis imperfecta and localised enamel defects
    T C Hart
    Department of Oral Medicine and Pathology, School of Dental Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    J Med Genet 40:900-6. 2003
    ..The phenotype associated with the g.13185_13186insAG ENAM mutation is dose dependent such that ARAI with openbite malocclusion segregates as a recessive trait, and enamel pitting as a dominant trait...
  51. pmc Synergistic roles of amelogenin and ameloblastin
    J Hatakeyama
    Functional Genomics Section, Laboratory of Cell and Developmental Biology, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA
    J Dent Res 88:318-22. 2009
    ..Ameloblastin-regulated expression of RhoGDI suggests that Rho-mediated signaling pathway might play a role in enamel formation...
  52. doi Candidate gene strategy reveals ENAM mutations
    H Y Kang
    Department of Cell and Developmental Biology, Dental Research Institute and BK21 Program, School of Dentistry, Seoul National University, Seoul, Korea
    J Dent Res 88:266-9. 2009
    ..14917delT, c.2991delT), which is predicted to result in a frameshift with a premature termination codon (p.L998fsX1062). This result supports the use of a candidate-gene-based strategy to study the genetic basis for AI...
  53. ncbi Characterization of Apin, a secreted protein highly expressed in tooth-associated epithelia
    Pierre Moffatt
    Genetics Unit, Shriners Hospital for Children, Montreal, Quebec, Canada H3G 1A6
    J Cell Biochem 103:941-56. 2008
    ..The presence of Apin at cell-tooth interfaces suggests involvement in adhesive mechanisms active at these sites, but its presence among other epithelial tissues indicates Apin likely possesses broader physiological roles...
  54. pmc FAM20: an evolutionarily conserved family of secreted proteins expressed in hematopoietic cells
    Demet Nalbant
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA
    BMC Genomics 6:11. 2005
    ..We have employed representational difference analysis to identify genes that are differentially expressed during experimentally induced myeloid differentiation in the murine EML hematopoietic stem cell line...
  55. ncbi Identification of a novel mutation in the enamalin gene in a family with autosomal-dominant amelogenesis imperfecta
    Sandra Janeth Gutierrez
    Dental Research Centre, School of Dentistry, Javeriana University, Bogota, Colombia
    Arch Oral Biol 52:503-6. 2007
    ..These results support a possible correlation between hypoplastic AI and mutations in the ENAM gene; however, identification of additional mutations could be helpful in establishing phenotype/genotype relationships...
  56. ncbi Effects of Emdogain on osteoblast gene expression
    F Carinci
    Department of Maxillofacial Surgery, University of Ferrara, Ferrara, Italy
    Oral Dis 12:329-42. 2006
    ..Enamel matrix proteins seem to be involved in root formation. EMD has been reported to promote proliferation, migration, adhesion and differentiation of cells associated with healing periodontal tissues in vivo...
  57. ncbi Amelogenin and ameloblastin show growth-factor like activity in periodontal ligament cells
    Maggie Zeichner-David
    Center for Craniofacial Molecular Biology, University of Southern California School of Dentistry, Los Angeles, CA 90033, USA
    Eur J Oral Sci 114:244-53; discussion 254-6, 381-2. 2006
    ..Amelogenin also induced the expression of bone sialoprotein. These results suggest that amelogenin, as well as ameloblastin, might have some 'growth factor' activity during periodontium development and regeneration...
  58. pmc Cleavage site specificity of MMP-20 for secretory-stage ameloblastin
    Y H P Chun
    Department of Biologic and Materials Sciences, University of Michigan, School of Dentistry, 1011 North University, Ann Arbor, MI 48109 1078, USA
    J Dent Res 89:785-90. 2010
    ..We conclude that MMP-20 is the enzyme that processes ameloblastin during the secretory stage of amelogenesis, and we present a hypothesis about the sequence of ameloblastin cleavages...
  59. ncbi In vitro effects of enamel matrix proteins on rat bone marrow cells and gingival fibroblasts
    S Keila
    Department of Oral Biology, The Maurice and Gabriela Goldschleger School of Dental Medicine, Tel Aviv University, Tel Aviv, Israel
    J Dent Res 83:134-8. 2004
    ..These in vitro data on BMSC and GF could explain the promotive effect of EMD on bone formation and connective tissue regeneration, respectively...
  60. ncbi Phenotype and enamel ultrastructure characteristics in patients with ENAM gene mutations g.13185-13186insAG and 8344delG
    Alenka Pavlic
    Department of Paediatric and Preventive Dentistry, Faculty of Medicine, University of Ljubljana, Hrvatski trg 6, 1000 Ljubljana, Slovenia
    Arch Oral Biol 52:209-17. 2007
    ..AI is associated with different mutations in four genes: enamelin (ENAM), amelogenin (AMGX), kallikrein (KLK4) and enamelysin (MMP-20). Seven different mutations have been identified in the enamelin gene (ENAM)...
  61. ncbi Processing of ameloblastin by MMP-20
    T Iwata
    Department of Biologic and Materials Sciences, Dental Research Lab, University of Michigan School of Dentistry, 1210 Eisenhower Place, Ann Arbor, MI 48108, USA
    J Dent Res 86:153-7. 2007
    ..We conclude that MMP-20 processes ameloblastin in vitro and in vivo...
  62. doi Inhibitory effect of enamel matrix derivative on osteoblastic differentiation of rat calvaria cells in culture
    Hideki Hama
    Department of Periodontology and Endodontology, Oral and Maxillofacial Dentistry, Division of Medico Dental Biosciences, Institute of Health Biosciences, University of Tokushima Graduate School, Tokushima 770 8504, Japan
    J Periodontal Res 43:179-85. 2008
    ..Transforming growth factor beta1 (TGF-beta1) is reported to be contained in EMD. The aim of this study was to clarify the effect of EMD on osteoblastic cell differentiation and the possible role of TGF-beta1...
  63. ncbi Transcription factor epiprofin is essential for tooth morphogenesis by regulating epithelial cell fate and tooth number
    Takashi Nakamura
    Laboratory of Cell and Developmental Biology, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland 20892 4370, USA
    J Biol Chem 283:4825-33. 2008
    ..Thus, Epfn has multiple functions for cell fate determination of the dental epithelium by regulating both proliferation and differentiation, preventing continuous tooth budding and generation...
  64. ncbi A mutation in the enamelin gene in a mouse model
    H Seedorf
    Department of Prosthetic Dentistry, University Medical Center, Hamburg Eppendorf, Martinistr 52, D 20246 Hamburg, Germany
    J Dent Res 86:764-8. 2007
    ..These results define the ATE1 mice as a model for local hypoplastic autosomal-dominant amelogenesis imperfecta (AIH2), which is caused by enamelin truncation mutations in humans...
  65. doi Clinical outcomes with bioactive agents alone or in combination with grafting or guided tissue regeneration
    Leonardo Trombelli
    Research Centre for the Study of Periodontal Diseases, University of Ferrara, Ferrara, Italy
    J Clin Periodontol 35:117-35. 2008
    ..The purpose of the present review was to determine the clinical effect of the use of bioactive agents (BAs) for the treatment of intra-osseous and furcation defects...
  66. ncbi A human X-Y homologous region encodes "amelogenin"
    Y Nakahori
    Department of Congenital Abnormalities Research, National Children s Medical Research Center, Tokyo, Japan
    Genomics 9:264-9. 1991
    ..Three exons are defined on both the X and Y sequences. The nucleotide sequences reported here offer basic information for investigating human amelogenin...
  67. ncbi Emdogain promotes osteoblast proliferation and differentiation and stimulates osteoprotegerin expression
    Jianing He
    Department of Endodontics, Baylor College of Dentistry, Waco, Texas, USA
    Oral Surg Oral Med Oral Pathol Oral Radiol Endod 97:239-45. 2004
    ....
  68. ncbi Autocrine growth factors in human periodontal ligament cells cultured on enamel matrix derivative
    S P Lyngstadaas
    Department Research and Development, BIORA AB, Malmo, Sweden
    J Clin Periodontol 28:181-8. 2001
    ..The cellular mechanism(s), e.g. autocrine growth factors, extracellular matrix synthesis and cell growth, underlying PDL regeneration with EMD is however poorly investigated...
  69. ncbi The influence of enamel matrix derivative associated with insulin-like growth factor-I on periodontal ligament fibroblasts
    Daniela B Palioto
    Department of Maxillo Facial Surgery and Periodontics, School of Dentistry of Ribeirao Preto, University of Sao Paulo, Brazil
    J Periodontol 75:498-504. 2004
    ..The objective of the present study was to evaluate the effect of EMD, IGF-I, and the combination of these two factors on the proliferation, adhesion, migration, and expression of type I collagen in PDL fibroblasts...
  70. ncbi Comparison of enamel matrix proteins and bioabsorbable membranes in the treatment of intrabony periodontal defects. A split-mouth study
    A Sculean
    Department of Periodontology and Conservative Dentistry, University of the Saarland, Homburg, Germany
    J Periodontol 70:255-62. 1999
    ..However, limited information is available concerning the comparison of the treatment of intrabony periodontal defects with enamel matrix proteins and other regenerative treatment alternatives...
  71. ncbi Effects of enamel matrix derivative and transforming growth factor-beta1 on human periodontal ligament fibroblasts
    Thaisângela L S Rodrigues
    Department of Bucco Maxillo Facial Surgery and Traumatology and Periodontology, School of Dentistry of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, SP, Brazil
    J Clin Periodontol 34:514-22. 2007
    ..The objective of this study was to evaluate the effects of enamel matrix derivative (EMD), transforming growth factor-beta1 (TGF-beta1), and a combination of both factors (EMD+TGF-beta1) on periodontal ligament (PDL) fibroblasts...
  72. ncbi In vitro studies on periodontal ligament cells and enamel matrix derivative
    S Gestrelius
    BIORA AB, Malmo, Sweden
    J Clin Periodontol 24:685-92. 1997
    ..With immunoassays used, none of these molecules were identified in EMD. These in vitro studies support the concept that EMD can act as a positive matrix for cells at a regenerative site...
  73. ncbi The developing enamel matrix: nature and function
    C Robinson
    Division of Oral Biology, Leeds Dental Institute, University of Leeds, UK
    Eur J Oral Sci 106:282-91. 1998
    ..Failure of these mechanisms could lead to incomplete maturation of the enamel and the eruption of dysplastic tissue...
  74. ncbi Enamel matrix derivative induces connective tissue growth factor expression in human osteoblastic cells
    Nora H M Heng
    Institute of Periodontology and Synoptic Dentistry, Charité Medical University of Berlin, Berlin, Germany
    J Periodontol 78:2369-79. 2007
    ..Also, the roles of CTGF in cell proliferation, cell cycle progression, and mineralized nodule formation of EMD-induced osteoblastic cultures were examined...
  75. ncbi Enamel matrix derivative and titanium implants
    V Franke Stenport
    Department of Biomaterials Handicap Research, Institute of Surgical Sciences, Goteborg University, Box 412, S 405 30 Goteborg, Sweden
    J Clin Periodontol 30:359-63. 2003
    ..The aim of present study was to evaluate if an enamel matrix derivative (Emdogain) may enhance bone formation and osseointegration of titanium implants, using a well-documented rabbit model...
  76. ncbi Emdogain-gel stimulates proliferation of odontoblasts and osteoblasts
    Jin Jiang
    Division of Endodontology, School of Dental Medicine, The University of Connecticut Health Center, Farmington, CT
    Oral Surg Oral Med Oral Pathol Oral Radiol Endod 102:698-702. 2006
    ..The purpose of this study was to determine whether a premixed form of enamel matrix derivative (EMD), Emdogain-gel, has the same property as the original formula of EMD in stimulating the proliferation of osteoblasts and odontoblasts...
  77. ncbi The effect of enamel matrix protein derivative on follicle cells in vitro
    S S Hakki
    Department of Periodontics/Prevention/Geriatrics, University of Michigan, School of Dentistry, Ann Arbor 48109-1078, USA
    J Periodontol 72:679-87. 2001
    ..Furthermore, EMD can regulate follicle cell activity, thus suggesting that epithelial-mesenchymal interactions may be important during development of periodontal tissues...
  78. ncbi Genes and related proteins involved in amelogenesis imperfecta
    G Stephanopoulos
    Diploma in Dental Science, Aristotle University of Thessaloniki, Greece
    J Dent Res 84:1117-26. 2005
    ....
  79. ncbi A new frameshift mutation encoding a truncated amelogenin leads to X-linked amelogenesis imperfecta
    S R Greene
    Department of Anatomy and Histology, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Arch Oral Biol 47:211-7. 2002
    ..While greater than 60% of the protein is predicted to be intact, the severity of this phenotype illustrates the importance of the C-terminal region of the amelogenin protein for the formation of enamel with normal thickness...
  80. pmc Identification and characterization of amelogenin genes in monotremes, reptiles, and amphibians
    S Toyosawa
    Max Planck Institut fur Biologie, Abteilung Immungenetik, Corrensstrasse 42, D 72076 Tubingen, Germany
    Proc Natl Acad Sci U S A 95:13056-61. 1998
    ..Clones corresponding to one locus only were found in caiman, whereas the clawed toad possesses at least two amelogenin-encoding loci...
  81. ncbi Identification and characterization of ameloblastin gene in a reptile
    Seikou Shintani
    Department of Pedodontics, Osaka University Graduate School of Dentistry, 1 8 Yamadaoka, Suita, 565 0871, Osaka, Japan
    Gene 283:245-54. 2002
    ..The caiman AMBN gene is a single-copy gene, transcribed only in the jaws, presumably in teeth...
  82. ncbi Reduced hydrolysis of amelogenin may result in X-linked amelogenesis imperfecta
    W Li
    Growth and Development Department, School of Dentistry, University of California San Francisco, 521 Parnassus Avenue, CA 94143-0640, USA
    Matrix Biol 19:755-60. 2001
    ..This study suggests that the reduced rate of TRAP formation due to a single amino acid substitution may alter enamel formation and consequently result in amelogenesis imperfecta...
  83. ncbi Amelogenesis imperfecta phenotype-genotype correlations with two amelogenin gene mutations
    P S Hart
    Department of Human Genetics, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA, USA
    Arch Oral Biol 47:261-5. 2002
    ..g. the mutation seen in family 1). This difference suggests that the C-terminus of the normal amelogenin protein is important for controlling enamel thickness...
  84. ncbi Clinical evaluation of an enamel matrix protein derivative (Emdogain) combined with a bovine-derived xenograft (Bio-Oss) for the treatment of intrabony periodontal defects in humans
    Anton Sculean
    Department of Periodontology and Conservative Dentistry, University of the Saarland, Homburg, Germany
    Int J Periodontics Restorative Dent 22:259-67. 2002
    ..However, no statistically significant differences in any of the investigated parameters were observed between the test and control groups. Both therapies led to significant improvements of the investigated clinical parameters...
  85. ncbi Evolution of the amelogenin gene in toothed and toothless vertebrates
    M Girondot
    URA 1137, CNRS and Universite Paris 7, France
    Eur J Oral Sci 106:501-8. 1998
    ..The amelogenin gene has been detected in all species except those lacking teeth. This result indicates that the unique role of amelogenin in amniotes is to contribute to enamel formation...
  86. ncbi A developmental comparison of matrix metalloproteinase-20 and amelogenin null mouse enamel
    John D Bartlett
    Department of Cytokine Biology, Forsyth Institute, Boston, MA 02115, USA
    Eur J Oral Sci 114:18-23; discussion 39-41, 379. 2006
    ..Both genes are essential for the generation of full-thickness enamel containing the characteristic decussating prism pattern...
  87. ncbi Amelogenin: lessons from evolution
    Jean Yves Sire
    Equipe Evolution and Développement du Squelette, CNRS FRE 2696, Université Paris 6 Pierre and Marie Curie, CNRS FRE 2696, Case 7077, 2, place Jussieu, 75251 Paris Cedex 05, France
    Arch Oral Biol 50:205-12. 2005
    ..Taken together these data suggest that amelogenin could have originated from either ameloblastin or enamelin, themselves being created from SPARCL1, which itself originated from a SPARC duplication, 600 millions years ago...
  88. ncbi Amelogenin-cytokeratin 14 interaction in ameloblasts during enamel formation
    R M Ravindranath
    Center for Craniofacial Molecular Biology, School of Dentistry, University of Southern California, Los Angeles, California 90033, USA
    J Biol Chem 276:36586-97. 2001
    ..Autoradiography with [(3)H]ATMP and [(3)H]GMp corroborated the dissociation of the co-assembly at the ameloblast Tomes' process. It is proposed that CK14 play a chaperon role for nascent amelogenin polypeptide during amelogenesis...
  89. pmc MMP20 active-site mutation in hypomaturation amelogenesis imperfecta
    D Ozdemir
    NIDCR National Institutes of Health, 10 Center Drive, Building 10, room 5 2531, Bethesda, MD 20892 1432, USA
    J Dent Res 84:1031-5. 2005
    ..H226Q). Zymogram analysis demonstrated that this missense mutation abolished MMP20 proteolytic activity. No MMP20 mutations were found in the remaining 14 probands, underscoring the genetic heterogeneity of hypomaturation AI...
  90. ncbi Is the amelogenin gene reliable for gender identification in forensic casework and prenatal diagnosis?
    K Thangaraj
    Centre for Cellular and Molecular Biology, Hyderabad, India
    Int J Legal Med 116:121-3. 2002
    ..SRY, STR and 50f2) would have been identified as females. Considering the consequences of the result obtained only using the amelogenin marker, we suggest the use of additional Y chromosome markers for unambiguous gender identification...
  91. ncbi Expression of ameloblastin during enamel formation in a crocodile
    Seikou Shintani
    Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, 1 8 Yamadaoka, Suita, Osaka 565 0871, Japan
    J Exp Zool B Mol Dev Evol 306:126-33. 2006
    ..The principal molecules that provide the adhesive function are presumably C-terminal peptides...
  92. ncbi Ameloblastin expression during craniofacial bone formation in rats
    Axel Spahr
    Department of Conservative Dentistry and Periodontology, University of Ulm, Ulm, Germany
    Eur J Oral Sci 114:504-11. 2006
    ..This study indicates the expression of the putative dental protein ameloblastin during craniofacial bone development in rats...
  93. ncbi Cloning, sequencing, and expression of the amelogenin gene in two scincid lizards
    S Delgado
    UMR 7138 Systématique, Adaptation, Evolution, Universite Paris 6, 7, quai St Bernard, 75005 Paris, France
    J Dent Res 85:138-43. 2006
    ..However, we have not found amelogenin transcripts in odontoblasts. This indicates that full-length amelogenin is specific to enamel matrix, at least in this lizard...
  94. pmc Exclusion of known gene for enamel development in two Brazilian families with amelogenesis imperfecta
    Maria C L G Santos
    Department of Morphology, Dental School of Piracicaba, State University of Campinas, Piracicaba, SP, Brazil
    Head Face Med 3:8. 2007
    ..These findings indicate extensive genetic heterogeneity for non-syndromic AI...
  95. ncbi The enamel protein amelogenin binds to the N-acetyl-D-glucosamine-mimicking peptide motif of cytokeratins
    R M Ravindranath
    Center for Craniofacial Molecular Biology, School of Dentistry, University of Southern California, Los Angeles, California 90033, USA
    J Biol Chem 275:39654-61. 2000
    ..This study documents that molecular mimicry may play a role in stability and organization of amelogenin during amelogenesis...
  96. ncbi Proteinases in developing dental enamel
    J D Bartlett
    Department of Biomineralization, Forsyth Institute, Boston, Massachusetts 02115, USA
    Crit Rev Oral Biol Med 10:425-41. 1999
    ..Other, as yet unidentified, proteinases and proteinase inhibitors are almost certainly present within the forming enamel and await discovery...
  97. ncbi In vitro biologic response of human bone marrow stromal cells to enamel matrix derivative
    Luigi Guida
    Department of Odontostomatological, Orthodontic and Surgical Disciplines, Second University of Naples, Naples, Italy
    J Periodontol 78:2190-6. 2007
    ..In this study, we investigated the effects of EMD on human BMSCs...
  98. ncbi Molecular analysis for genetic counselling in amelogenesis imperfecta
    M J Aldred
    Department of Dentistry, Royal Children s Hospital, Melbourne, Australia
    Oral Dis 8:249-53. 2002
    ..To use molecular genetics to establish the mode of inheritance in a family with amelogenesis imperfecta...
  99. ncbi Exclusion of candidate genes in two families with autosomal dominant hypocalcified amelogenesis imperfecta
    P Suzanne Hart
    Department of Genetics, Graduate School of Public Health, University of Pittsburgh, Pittsburgh PA 15261, USA
    Eur J Oral Sci 111:326-31. 2003
    ....
  100. ncbi Tuftelin--aspects of protein and gene structure
    D Deutsch
    Department of Oral Biology, Hebrew University, Hadassah Faculty of Dental Medicine, Jerusalem, Israel
    Eur J Oral Sci 106:315-23. 1998
    ..The timing of tuftelin expression and its possible roles in enamel formation are discussed...
  101. ncbi Effect of enamel matrix protein derivative on the attachment, proliferation, and viability of human SaOs(2) osteoblasts on titanium implants
    Frank Schwarz
    Department of Oral Surgery, Heinrich Heine University, Westdeutsche Kieferklinik, Moorenstrasse 5, 40225 Dusseldorf, Germany
    Clin Oral Investig 8:165-71. 2004
    ..Within the limits of the present study, it was concluded that EMD enhanced cell proliferation and viability of human SaOs(2) osteoblasts on SLA titanium implants in a concentration-dependent manner...

Research Grants100

  1. Biomimetic Synthesis of an Enamel-Like Material
    Stefan Habelitz; Fiscal Year: 2004
    ..abstract_text> ..
  2. ENAMEL MINERAL FORMATION DURING MURINE ODONTOGENESIS
    Carolyn W Gibson; Fiscal Year: 2010
    ..Mouse models mimic the human defects and can be used to correlate individual gene mutations with appropriate clinical intervention. ..
  3. REGULATION OF EXPRESSION OF THE AMELOGENIN GENES
    CAROLYN GIBSON; Fiscal Year: 1993
    ....
  4. ENAMEL MINERAL FORMATION DURING MURINE ODONTOGENESIS
    CAROLYN GIBSON; Fiscal Year: 2006
    ..3) to evaluate LRAP for signal function in vivo and in vitro; and (4) to ascertain additional consequences of absence of amelogenin in maturative ameloblasts, in reparative dentin and during accelerated eruption ..
  5. The Impact of Fluoride on the Rho Signaling Pathway and the Actin Cytoskeleton
    CAROLYN GIBSON; Fiscal Year: 2007
    ..A better understanding of the health-related benefits and problems associated with environmental fluoride will lead to more informed policy decisions and fundamental knowledge about the impact of this environmental agent. ..
  6. ENAMEL MINERAL FORMATION DURING MURINE ODONTOGENESIS
    CAROLYN GIBSON; Fiscal Year: 2009
    ..Mouse models mimic the human defects and can be used to correlate individual gene mutations with appropriate clinical intervention. ..
  7. REGULATION OF EXPRESSION OF THE AMELOGENIN GENES
    CAROLYN GIBSON; Fiscal Year: 2001
    ..This is a unique opportunity to study regulation of a sexually dimorphic gene that has a completely tissue specific pattern of expression. ..
  8. ENAMEL MINERAL FORMATION DURING MURINE ODONTOGENESIS
    CAROLYN GIBSON; Fiscal Year: 2009
    ..Mouse models mimic the human defects and can be used to correlate individual gene mutations with appropriate clinical intervention. ..
  9. MATRIX-BASED MINERAL (MBM) ENAMEL BIOMIMETICS
    JANET OLDAK; Fiscal Year: 2004
    ....
  10. ACTION AND FUNCTION OF MMP-20 DURING ENAMEL FORMATION
    JANET OLDAK; Fiscal Year: 2007
    ..abstract_text> ..
  11. AMELOGENIN PROCESSING AND APATITE CRYSTAL GROWTH
    JANET OLDAK; Fiscal Year: 2002
    ....
  12. MATRIX BASED MINERAL ENAMEL-BIOMIMETICS
    JANET M OLDAK; Fiscal Year: 2010
    ..Moreover, this study will have a significant impact on the field of biomineralization, molecular self-assembly, protein structure, and understanding of pathological enamel formation. ..
  13. MATRIX BASED MINERAL ENAMEL-BIOMIMETICS
    JANET OLDAK; Fiscal Year: 2009
    ..Moreover, this study will have a significant impact on the field of biomineralization, molecular self-assembly, protein structure, and understanding of pathological enamel formation. ..
  14. A NEW STRATEGY FOR AMELOGENIN CYRSTALLIZATION
    JANET OLDAK; Fiscal Year: 2004
    ..abstract_text> ..
  15. INTRINSICALLY DISORDERED PROTEINS IN BIOMINERALIZATION
    JANET M OLDAK; Fiscal Year: 2010
    ..The outcomes of our study will therefore have the potential to improve treatments for repair and regeneration of oral, dental and craniofacial tissues. ..
  16. MATRIX BASED MINERAL ENAMEL-BIOMIMETICS
    JANET OLDAK; Fiscal Year: 2009
    ..Moreover, this study will have a significant impact on the field of biomineralization, molecular self-assembly, protein structure, and understanding of pathological enamel formation. ..
  17. LECTIN-LIKE PROPERTIES OF AMELOGENINS
    RAJESWARI RAVINDRANATH; Fiscal Year: 2004
    ..These experiments are a logical extension of the newly identified lectin-like properties of amelogenins (Ravindranath et al. Submitted), which are postulated to play a functional role in normal enamel biomineralization. ..
  18. Why is Fam83h critical for enamel formation?
    Jan C C Hu; Fiscal Year: 2010
    ..Our long term goal is to advance understanding of normal and pathological tooth formation to stimulate improvements in the public's oral health. ..
  19. Phenotype and Gene Expression in Odontogenic Tumors
    JOHN T WRIGHT; Fiscal Year: 2010
    ..Gene data will be placed on our web site in a timely fashion for data sharing. These studies will provide the first detailed mutational and gene profile studies of human odontogenic tumors. ..
  20. MODIFYING GENE DETERMINANTS OF TOOTH AND BONE PHENOTYPES
    John Wright; Fiscal Year: 2007
    ..abstract_text> ..
  21. Enamelysin Processing Mechanisms in Amelogenesis
    John Bartlett; Fiscal Year: 2007
    ....
  22. Matrix Component Interactions in Bone, Dentin, and Invertebrate Mineral
    Arthur Veis; Fiscal Year: 2010
    ..Invertebrate mineralization studied from a similar perspective links into the same conceptual framework with implications in developmental and evolutionary biology, and biomimetic tissue repair models. ..
  23. MATRIX COMPONENT INTERACTIONS IN BONE AND DENTIN
    Arthur Veis; Fiscal Year: 2005
    ..These data should provide a clearer understanding of the mechanisms whereby the acidic matrix proteins nucleate and control mineral deposition, and link the vertebrate and invertebrate systems. ..
  24. THE GENOTYPE AND PHENOTYPE AMELOGENESIS IMPERFECTA
    John Wright; Fiscal Year: 2004
    ..Knowledge of the molecular determinants of morphogenesis and tissue formation will allow novel and more effective treatments and more accurate diagnosis of the diverse hereditary conditions affecting teeth. ..
  25. MATRIX COMPONENT INTERACTIONS IN BONE AND DENTIN
    Arthur Veis; Fiscal Year: 2002
    ..The Dmp2 gene has been co-localized with the mineralization disorder Dentinogenesis Imperfecta Type II (DI-II) on human chromosome 4q21, and its possible relationship with DI-II will be explored. ..
  26. Phenotype and Gene Expression in Odontogenic Tumors
    John Wright; Fiscal Year: 2007
    ..Gene data will be placed on our web site in a timely fashion for data sharing. These studies will provide the first detailed mutational and gene profile studies of human odontogenic tumors. ..
  27. Recombinant Amelogenin Matrices for Apatite Nanofibers
    Stefan Habelitz; Fiscal Year: 2007
    ..Importantly, comprehending nanoscale control over this complex process will provide a basis for development of novel methods for mineralized tissue repair. ..
  28. Solid State NMR Structure/Function Studies of Amelogenin
    WENDY SHAW; Fiscal Year: 2007
    ..These studies will yield an in depth understanding of the molecular level processes involved in the formation of teeth, and more generally will provide basic insight into protein/crystal interactions. ..
  29. Solid State NMR Structure/Function Studies of Amelogenin
    Wendy J Shaw; Fiscal Year: 2010
    ....
  30. Enamelysin Processing Mechanisms in Amelogenesis
    John Bartlett; Fiscal Year: 2009
    ..This proposal seeks to demonstrate that cathepsin K plays an essential role in enamel formation by degrading enamel matrix proteins as the proteins are removed from the maturing enamel. ..
  31. The Role of ER-stress and pH in Fluorosis
    John Bartlett; Fiscal Year: 2009
    ..This application seeks to identify those stress response genes and determine if they play a role in causing Dental fluorosis. ..
  32. A Unified Surgical Instrument System for Periodontal Minimally Invasive Surgery
    STEPHEN HARREL; Fiscal Year: 2007
    ..The resulting system, based on the surgical consultants evaluations and scores, will be used in a Phase 2 multicenter controlled clinical evaluation of MIS. ..
  33. MMP-20 AND MMP-20 DOMAIN FUNCTION IN FORMING ENAMEL
    John Bartlett; Fiscal Year: 2006
    ..This application builds on results from a highly productive R29 grant (DE12098). ..