contractile proteins

Summary

Summary: Proteins which participate in contractile processes. They include MUSCLE PROTEINS as well as those found in other cells and tissues. In the latter, these proteins participate in localized contractile events in the cytoplasm, in motile activity, and in cell aggregation phenomena.

Top Publications

  1. pmc Citron kinase controls abscission through RhoA and anillin
    Marta Gai
    Department of Genetics, Biology, and Biochemistry, Molecular Biotechnology Center, University of Turin, 10126 Turin, Italy
    Mol Biol Cell 22:3768-78. 2011
  2. ncbi Self- and actin-templated assembly of Mammalian septins
    Makoto Kinoshita
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Dev Cell 3:791-802. 2002
  3. ncbi Mena, a relative of VASP and Drosophila Enabled, is implicated in the control of microfilament dynamics
    F B Gertler
    Division of Molecular Medicine, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA
    Cell 87:227-39. 1996
  4. ncbi Cleavage furrow organization requires PIP(2)-mediated recruitment of anillin
    Jinghe Liu
    Department of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, Canada
    Curr Biol 22:64-9. 2012
  5. pmc Anillin acts as a bifunctional linker coordinating midbody ring biogenesis during cytokinesis
    Amel Kechad
    CHU Sainte Justine Centre de Recherche, Centre de cancérologie Charles Bruneau, 3175 Chemin de la Côte Ste Catherine, Montreal, Quebec H3T 1C5, Canada
    Curr Biol 22:197-203. 2012
  6. pmc Filamin A regulates focal adhesion disassembly and suppresses breast cancer cell migration and invasion
    Yingjie Xu
    Lady Davis Institute for Medical Research, Jewish General Hospital, Faculty of Medicine, McGill University, Montreal, Quebec H3T 1E2, Canada
    J Exp Med 207:2421-37. 2010
  7. ncbi Dissecting temporal and spatial control of cytokinesis with a myosin II Inhibitor
    Aaron F Straight
    Department of Cell Biology and Institute of Chemistry and Cell Biology, Harvard Medical School, 250 Longwood Avenue, Boston, MA 02115, USA
    Science 299:1743-7. 2003
  8. pmc Temporal control of contractile ring assembly by Plo1 regulation of myosin II recruitment by Mid1/anillin
    Maria Almonacid
    Institut Curie, Centre de Recherche F 75248 Paris, France
    Curr Biol 21:473-9. 2011
  9. ncbi Bni1p, a yeast formin linking cdc42p and the actin cytoskeleton during polarized morphogenesis
    M Evangelista
    Institute of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia V5A 1S6, Canada
    Science 276:118-22. 1997
  10. pmc The filamins: organizers of cell structure and function
    Fumihiko Nakamura
    Translational Medicine Division, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA, USA
    Cell Adh Migr 5:160-9. 2011

Detail Information

Publications329 found, 100 shown here

  1. pmc Citron kinase controls abscission through RhoA and anillin
    Marta Gai
    Department of Genetics, Biology, and Biochemistry, Molecular Biotechnology Center, University of Turin, 10126 Turin, Italy
    Mol Biol Cell 22:3768-78. 2011
    ..Altogether, these results indicate that CIT-K is a crucial abscission regulator that may promote midbody stability through active RhoA and anillin...
  2. ncbi Self- and actin-templated assembly of Mammalian septins
    Makoto Kinoshita
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Dev Cell 3:791-802. 2002
    ..We conclude that septins alone self-assemble into rings, that adaptor proteins recruit septins to actin bundles, and that septins help organize these bundles...
  3. ncbi Mena, a relative of VASP and Drosophila Enabled, is implicated in the control of microfilament dynamics
    F B Gertler
    Division of Molecular Medicine, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA
    Cell 87:227-39. 1996
    ..Expression of neural-enriched isoforms of Mena in fibroblasts induces the formation of abnormal F-actin-rich outgrowths, supporting a role for this protein in microfilament assembly and cell motility...
  4. ncbi Cleavage furrow organization requires PIP(2)-mediated recruitment of anillin
    Jinghe Liu
    Department of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, Canada
    Curr Biol 22:64-9. 2012
    ..These data demonstrate that the anillin PH domain has two functions: targeting anillin to the furrow by binding to PI(4,5)P(2) to maintain furrow organization and recruiting septins to the furrow...
  5. pmc Anillin acts as a bifunctional linker coordinating midbody ring biogenesis during cytokinesis
    Amel Kechad
    CHU Sainte Justine Centre de Recherche, Centre de cancérologie Charles Bruneau, 3175 Chemin de la Côte Ste Catherine, Montreal, Quebec H3T 1C5, Canada
    Curr Biol 22:197-203. 2012
    ..One apparently acts as the core structural template for MR assembly, while the other ensures stable anchoring of the plasma membrane beyond the CR stage...
  6. pmc Filamin A regulates focal adhesion disassembly and suppresses breast cancer cell migration and invasion
    Yingjie Xu
    Lady Davis Institute for Medical Research, Jewish General Hospital, Faculty of Medicine, McGill University, Montreal, Quebec H3T 1E2, Canada
    J Exp Med 207:2421-37. 2010
    ..These results document a regulation of FA dynamics by FLNa in breast cancer cells...
  7. ncbi Dissecting temporal and spatial control of cytokinesis with a myosin II Inhibitor
    Aaron F Straight
    Department of Cell Biology and Institute of Chemistry and Cell Biology, Harvard Medical School, 250 Longwood Avenue, Boston, MA 02115, USA
    Science 299:1743-7. 2003
    ..Continuous signals from microtubules are required to maintain the position of the cleavage furrow, and these signals control the localization of myosin II independently of other furrow components...
  8. pmc Temporal control of contractile ring assembly by Plo1 regulation of myosin II recruitment by Mid1/anillin
    Maria Almonacid
    Institut Curie, Centre de Recherche F 75248 Paris, France
    Curr Biol 21:473-9. 2011
    ..Plo1 thereby facilitates contractile ring assembly at mitotic onset...
  9. ncbi Bni1p, a yeast formin linking cdc42p and the actin cytoskeleton during polarized morphogenesis
    M Evangelista
    Institute of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia V5A 1S6, Canada
    Science 276:118-22. 1997
    ..Both Bni1p and Bud6p (like Cdc42p and actin) localized to the tips of mating projections. Bni1p may function as a Cdc42p target that links the pheromone response pathway to the actin cytoskeleton...
  10. pmc The filamins: organizers of cell structure and function
    Fumihiko Nakamura
    Translational Medicine Division, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA, USA
    Cell Adh Migr 5:160-9. 2011
    ..Due to this diversity, mutations in human FLN genes result in a wide range of anomalies with moderate to lethal consequences. This review focuses on the structure and functions of FLNa in cell migration and adhesion...
  11. pmc Filamin-A as a marker and target for DNA damage based cancer therapy
    Jingyin Yue
    Department of Radiation Oncology, The Cancer Institute of New Jersey, UMDNJ Robert Wood Johnson Medical School, 195 Little Albany St, New Brunswick, NJ 08903, USA
    DNA Repair (Amst) 11:192-200. 2012
    ..Thus filamin-A can be used as a biomarker and a target for DNA damage based cancer therapy...
  12. pmc Molecular dissection of cytokinesis by RNA interference in Drosophila cultured cells
    Maria Patrizia Somma
    Istituto Pasteur Fondazione Cenci Bolognetti and Centro di Genetica Evoluzionistica del Consiglio Nazionale delle Richerche, Dipartimento di Genetica e Biologia Molecolare, Universita di Roma La Sapienza, 00185 Rome, Italy
    Mol Biol Cell 13:2448-60. 2002
    ....
  13. ncbi How to scaffold the contractile ring for a safe cytokinesis - lessons from Anillin-related proteins
    Pier Paolo D'Avino
    Department of Pathology, University of Cambridge, Cambridge CB2 1QP, UK
    J Cell Sci 122:1071-9. 2009
    ....
  14. pmc Filamin A (FLNA) is required for cell-cell contact in vascular development and cardiac morphogenesis
    Yuanyi Feng
    Division of Genetics and Department of Cardiology, Children s Hospital Boston, Boston, MA 02215, USA
    Proc Natl Acad Sci U S A 103:19836-41. 2006
    ..Essential roles for FLNA in intercellular junctions provide a mechanism for the diverse developmental defects seen in patients with FLNA mutations...
  15. ncbi Direct observation of dendritic actin filament networks nucleated by Arp2/3 complex and WASP/Scar proteins
    L Blanchoin
    Structural Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037, USA
    Nature 404:1007-11. 2000
    ..These properties result in the automatic assembly of the branched actin network after activation by proteins of the WASP/Scar family and favour the selective disassembly of proximal regions of the network...
  16. pmc Identification of filamin as a novel ligand for caveolin-1: evidence for the organization of caveolin-1-associated membrane domains by the actin cytoskeleton
    M Stahlhut
    Structural Cell Biology Unit, Department of Medical Anatomy, The Panum Institute, University of Copenhagen, DK 2200 Copenhagen N, Denmark
    Mol Biol Cell 11:325-37. 2000
    ..The actin network, therefore, seems to be directly involved in the spatial organization of caveolin-1-associated membrane domains...
  17. pmc Disruption of neural progenitors along the ventricular and subventricular zones in periventricular heterotopia
    Russell J Ferland
    Department of Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA
    Hum Mol Genet 18:497-516. 2009
    ..Our current findings suggest that PH formation arises from a final common pathway involving disruption of vesicle trafficking, leading to impaired cell adhesion and loss of neuroependymal integrity...
  18. pmc Mutations in orbit/mast reveal that the central spindle is comprised of two microtubule populations, those that initiate cleavage and those that propagate furrow ingression
    Yoshihiro H Inoue
    Drosophila Genetic Resource Center, Kyoto Institute of Technology, Sagaippongi cho, Ukyou ku, 616 8354, Japan
    J Cell Biol 166:49-60. 2004
    ..Our work identifies a new function for Orbit/Mast/CLASP and identifies a novel MT population involved in cleavage furrow initiation...
  19. ncbi Structural and functional aspects of filamins
    A van Der Flier
    Division of Cell Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands
    Biochim Biophys Acta 1538:99-117. 2001
    ..In this review aspects of filamin structure, its splice variants, binding partners and biological function will be discussed...
  20. pmc Filamin A regulates caveolae internalization and trafficking in endothelial cells
    Maria Sverdlov
    Department of Pharmacology, University of Illinois at Chicago, Chicago, IL 60612, USA
    Mol Biol Cell 20:4531-40. 2009
    ..Taken together, these data suggest that filamin A association with caveolin-1 promotes caveolae-mediated transport by regulating vesicle internalization, clustering, and trafficking...
  21. ncbi Molecular mechanisms controlling actin filament dynamics in nonmuscle cells
    T D Pollard
    Structural Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037, USA
    Annu Rev Biophys Biomol Struct 29:545-76. 2000
    ..Profilin catalyzes exchange of ADP for ATP, recycling actin back to a pool of unpolymerized monomers bound to profilin and thymosin-beta 4 that is poised for rapid elongation of new barbed ends...
  22. pmc An anillin-Ect2 complex stabilizes central spindle microtubules at the cortex during cytokinesis
    PAUL FRENETTE
    Department of Biology, Concordia University, Montreal, Quebec, Canada
    PLoS ONE 7:e34888. 2012
    ....
  23. pmc Activation of the CDC42 effector N-WASP by the Shigella flexneri IcsA protein promotes actin nucleation by Arp2/3 complex and bacterial actin-based motility
    C Egile
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Cell Biol 146:1319-32. 1999
    ..VASP is not involved in Shigella movement, and the function of profilin does not require its binding to proline-rich regions...
  24. pmc The cytoskeleton protein filamin-A is required for an efficient recombinational DNA double strand break repair
    Jingyin Yue
    Department of Radiation Oncology, The Cancer Institute of New Jersey, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, New Brunswick, New Jersey 08903, USA
    Cancer Res 69:7978-85. 2009
    ....
  25. pmc Embryonic morphogenesis in Caenorhabditis elegans integrates the activity of LET-502 Rho-binding kinase, MEL-11 myosin phosphatase, DAF-2 insulin receptor and FEM-2 PP2c phosphatase
    A J Piekny
    Genes and Development Research Group and Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta T2N 4N1, Canada
    Genetics 156:1671-89. 2000
    ..fem-2 blocked the near-normal elongation of let-502; mel-11 indicating that fem-2 acts in a parallel elongation pathway. The action of two redundant pathways likely ensures accurate elongation of the C. elegans embryo...
  26. ncbi Filamin B mutations cause chondrocyte defects in skeletal development
    Jie Lu
    Department of Neurology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, USA
    Hum Mol Genet 16:1661-75. 2007
    ..These data suggest that disruption of the ECM-beta1-integrin-Flnb pathway contributes to defects in vertebral and distal limb development, similar to those seen in the human autosomal recessive SCT due to Flnb mutations...
  27. ncbi chickadee encodes a profilin required for intercellular cytoplasm transport during Drosophila oogenesis
    L Cooley
    Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06510
    Cell 69:173-84. 1992
    ..We suggest that the newly synthesized cytoplasmic actin networks are responsible for maintaining nuclear position in the nurse cells...
  28. pmc Loss of FilaminC (FLNc) results in severe defects in myogenesis and myotube structure
    I Dalkilic
    Howard Hughes Medical Institute and Program in Genomics, Children s Hospital, Boston, Massachusetts 02115, USA
    Mol Cell Biol 26:6522-34. 2006
    ..These data provide the first evidence that FLNc has a crucial role in muscle development and maintenance of muscle structural integrity and suggest the presence of a TRIO-FLNc-dependent pathway in maintaining proper myotube structure...
  29. pmc Myofibrillar myopathies
    Duygu Selcen
    Department of Neurology, Division of Child Neurology and Neuromuscular Disease Research Laboratory, Mayo Clinic College of Medicine, Mayo Clinic, Rochester, MN, USA
    Neuromuscul Disord 21:161-71. 2011
    ..However, in the majority of the myofibrillar myopathy patients the disease gene awaits discovery...
  30. pmc pavarotti encodes a kinesin-like protein required to organize the central spindle and contractile ring for cytokinesis
    R R Adams
    Cancer Research Campaign CRC Laboratories, Cell Cycle Genetics Research Group, Department of Anatomy and Physiology, Medical Sciences Institute, University of Dundee, Dundee DD1 4HN, UK
    Genes Dev 12:1483-94. 1998
    ..We suggest that PAV-KLP is required both to establish the structure of the telophase spindle to provide a framework for the assembly of the contractile ring, and to mobilize mitotic regulator proteins...
  31. ncbi Filamins as integrators of cell mechanics and signalling
    T P Stossel
    Brigham and Women s Hospital, Boston, Massachusetts 02115, USA
    Nat Rev Mol Cell Biol 2:138-45. 2001
    ..They integrate cellular architectural and signalling functions and are essential for fetal development and cell locomotion. Here, we describe the history, structure and function of this group of proteins...
  32. ncbi The myriad roles of Anillin during cytokinesis
    Alisa J Piekny
    Department of Biology, Concordia University, Montreal, QC, Canada
    Semin Cell Dev Biol 21:881-91. 2010
    ..Here we review Anillin's interacting partners and the relevance of these interactions in vivo. We also discuss questions of how these interactions are coordinated, and finally provide some perspective regarding Anillin's role in cancer...
  33. pmc Filamin B plays a key role in vascular endothelial growth factor-induced endothelial cell motility through its interaction with Rac-1 and Vav-2
    Beatriz Del Valle-Pérez
    From the Unitat de Bioquímica i Biologia Molecular, Departament de Ciències Fisiològiques II, Universitat de Barcelona IDIBELL, E 08907 Barcelona, Spain
    J Biol Chem 285:10748-60. 2010
    ..In conclusion, our results reveal a crucial role for FLNB in endothelial cell migration and in the angiogenic process in adult endothelial cells...
  34. ncbi Recovery from DNA damage-induced G2 arrest requires actin-binding protein filamin-A/actin-binding protein 280
    Xiangbing Meng
    Department of Molecular Genetics and Microbiology, MSC08 4660, 1 University of New Mexico School of Medicine, Albuquerque, New Mexico 87131 0001, USA
    J Biol Chem 279:6098-105. 2004
    ..Together, these data suggest a functional role of filamin-A in the recovery from G2 arrest and subsequent mitotic cell death after DNA damage...
  35. ncbi Migfilin and Mig-2 link focal adhesions to filamin and the actin cytoskeleton and function in cell shape modulation
    Yizeng Tu
    Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Cell 113:37-47. 2003
    ..Together, Mig-2, migfilin and filamin define a connection between cell matrix adhesions and the actin cytoskeleton and participate in the orchestration of actin assembly and cell shape modulation...
  36. pmc Exportin 6: a novel nuclear export receptor that is specific for profilin.actin complexes
    Theis Stüven
    ZMBH, INF 282, D 69120 Heidelberg and Universität Lübeck, Ratzeburger Allee 160, D 23538 Lubeck, Germany
    EMBO J 22:5928-40. 2003
    ..In contrast to a previous report, we found no indications of a major and direct role for CRM1 in actin export from mammalian or insect nuclei...
  37. ncbi Increased Ca2+-sensitivity of the contractile apparatus in end-stage human heart failure results from altered phosphorylation of contractile proteins
    J van der Velden
    Laboratory for Physiology, Institute for Cardiovascular Research ICaR VU, VU University Medical Center, Van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands
    Cardiovasc Res 57:37-47. 2003
    The alterations in contractile proteins underlying enhanced Ca(2+)-sensitivity of the contractile apparatus in end-stage failing human myocardium are still not resolved...
  38. pmc Reassembly of contractile actin cortex in cell blebs
    Guillaume T Charras
    Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA
    J Cell Biol 175:477-90. 2006
    ..ezrin was recruited to the membrane first, followed by actin, actin-bundling proteins, and, finally, contractile proteins. Complete assembly of the contractile cortex, which was organized into a cagelike mesh of filaments, took ..
  39. pmc Mid1p/anillin and the septation initiation network orchestrate contractile ring assembly for cytokinesis
    Olivier Hachet
    Cell Cycle Control Laboratory, Swiss Institute for Experimental Cancer Research ISREC, School of Life Sciences, Ecole Polytechnique Federale de Lausanne EPFL, 1066 Epalinges s Lausanne, Switzerland
    Genes Dev 22:3205-16. 2008
    ..Taken together, these data establish that Mid1p and the SIN are the key regulators that orchestrate CAR assembly...
  40. ncbi Proteomic analysis in aortic media of patients with Marfan syndrome reveals increased activity of calpain 2 in aortic aneurysms
    Christiane Pilop
    Departments of Nephrology and Hypertension, University Hospital of Bern, Bern, Switzerland
    Circulation 120:983-91. 2009
    ..The most life-threatening manifestations are aortic aneurysm and dissection. We investigated changes in the proteome of aortic media in patients with and without MFS to gain insight into molecular mechanisms leading to aortic dilatation...
  41. pmc Filamins regulate cell spreading and initiation of cell migration
    Massimiliano Baldassarre
    Department of Pharmacology and Interdepartmental Program in Vascular Biology and Therapeutics, Yale University School of Medicine, New Haven, CT, USA
    PLoS ONE 4:e7830. 2009
    ..We propose that interactions between FLNs and transmembrane or signalling proteins, mediated at least in part by immunoglobulin domains 19 to 21 are important for both cell spreading and initiation of migration...
  42. pmc An active biopolymer network controlled by molecular motors
    Gijsje H Koenderink
    Department of Physics and Harvard School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138
    Proc Natl Acad Sci U S A 106:15192-7. 2009
    ..This design principle may be applicable to engineering novel biologically inspired, active materials that adjust their own stiffness by internal catalytic control...
  43. ncbi Actin machinery: pushing the envelope
    G G Borisy
    Laboratory of Molecular Biology, University of Wisconsin, Madison, WI 53706, USA
    Curr Opin Cell Biol 12:104-12. 2000
    ..Instead, actin polymerization, in conjunction with a small number of proteins, is sufficient. A dendritic pattern of nucleation controlled by the Arp2/3 complex provides an efficient pushing force for lamellipodial motility...
  44. pmc Role of proteins of the Ena/VASP family in actin-based motility of Listeria monocytogenes
    V Laurent
    Dynamique du Cytosquelette, Laboratoire d Enzymologie et Biochimie Structurales, CNRS, 91198 Gif sur Yvette, France
    J Cell Biol 144:1245-58. 1999
    ..We propose a molecular ratchet model for insertional polymerization of actin, within which frequent attachment-detachment of VASP to F-actin allows its sliding along the growing filament...
  45. pmc Androgen-induced cell migration: role of androgen receptor/filamin A association
    Gabriella Castoria
    Dipartimento di Patologia Generale, II Universita di Napoli, Napoli, Italy
    PLoS ONE 6:e17218. 2011
    ..These findings support a role for AR in cell migration and invasiveness. However, the molecular mechanism involved in AR-mediated cell migration still remains elusive...
  46. pmc The small GTPase RalA targets filamin to induce filopodia
    Y Ohta
    Division of Biochemistry and Cellular Biology, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4 1 1 Ogawahigashi, Kodaira, Tokyo 187, Japan
    Proc Natl Acad Sci U S A 96:2122-8. 1999
    ..RalA therefore is a downstream intermediate in Cdc42-mediated filopod production and uses filamin in this pathway...
  47. pmc Nuclear versus cytoplasmic localization of filamin A in prostate cancer: immunohistochemical correlation with metastases
    Roble G Bedolla
    University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA
    Clin Cancer Res 15:788-96. 2009
    ..We have examined whether FlnA localization determines a propensity to metastasis in advanced androgen-independent prostate cancer...
  48. ncbi Mutations in filamin 1 prevent migration of cerebral cortical neurons in human periventricular heterotopia
    J W Fox
    Department of Neurology, Beth Israel Deaconess Medical Center, Harvard Institutes of Medicine, Boston, Massachusetts 02115, USA
    Neuron 21:1315-25. 1998
    ..FLN1 shows previously unrecognized, high-level expression in the developing cortex, is required for neuronal migration to the cortex, and is essential for embryogenesis...
  49. ncbi Filamins in cell signaling, transcription and organ development
    Alex Xianghua Zhou
    Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, University of Gothenburg, SE 405 30 Goteborg, Sweden
    Trends Cell Biol 20:113-23. 2010
    ..This review focuses on the emerging roles of filamins in cell signaling and transcription, with emphasis on cell motility and organ development...
  50. ncbi Drosophila filamin encoded by the cheerio locus is a component of ovarian ring canals
    N S Sokol
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06510, USA
    Curr Biol 9:1221-30. 1999
    ..Female sterile cheerio mutants fail to recruit actin to ring canals, disrupting the flow of cytoplasm to oocytes...
  51. ncbi Isolation and characterization of fission yeast mutants defective in the assembly and placement of the contractile actin ring
    F Chang
    Imperial Cancer Research Fund, London, UK
    J Cell Sci 109:131-42. 1996
    ..The actin ring forms medially in a beta-tubulin mutant, showing that actin ring formation and placement are not dependent on the mitotic spindle...
  52. ncbi The Rac1- and RhoG-specific GEF domain of Trio targets filamin to remodel cytoskeletal actin
    J M Bellanger
    CRBM CNRS, UPR 1086, 1919 Route de Mende, 34293 Montpellier Cedex 5, France
    Nat Cell Biol 2:888-92. 2000
    ..In addition, Trio GEFD1 exchange activity is not affected by filamin binding. Our results indicate that filamin, as a molecular target of Trio, may be a scaffold for the spatial organization of Rho-GTPase-mediated signalling pathways...
  53. pmc In mouse brain profilin I and profilin II associate with regulators of the endocytic pathway and actin assembly
    W Witke
    Mouse Biology Programme, EMBL, Monterotondo Rome, Italy
    EMBO J 17:967-76. 1998
    ..Our findings strongly suggest that in brain profilin I and profilin II complexes link the actin cytoskeleton and endocytic membrane flow, directing actin and clathrin assembly to distinct membrane domains...
  54. pmc Absence of filamin A prevents cells from responding to stiffness gradients on gels coated with collagen but not fibronectin
    Fitzroy J Byfield
    Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Biophys J 96:5095-102. 2009
    ..These results have implications for different models of cell protrusion and adhesion and identify a key role for filamin A in altering cellular stiffness that cannot be compensated for by other actin cross-linkers in vivo...
  55. ncbi Studies of focal adhesion assembly
    Iain D Campbell
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
    Biochem Soc Trans 36:263-6. 2008
    ....
  56. ncbi Mutations in the X-linked filamin 1 gene cause periventricular nodular heterotopia in males as well as in females
    V L Sheen
    Department of Neurology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02115, USA
    Hum Mol Genet 10:1775-83. 2001
    ..These results demonstrate the sensitivity and specificity of DNA testing for FLN1 mutations and have important functional implications for models of FLN1 protein function in neuronal migration...
  57. ncbi Reconstitution of actin-based motility of Listeria and Shigella using pure proteins
    T P Loisel
    Dynamique du Cytosquelette, LEBS, CNRS, Gif sur Yvette, France
    Nature 401:613-6. 1999
    ..The movement is more effective when profilin, alpha-actinin and VASP (for Listeria) are also included. These results have implications for our understanding of the mechanism of actin-based motility in cells...
  58. ncbi Regulation of contractile proteins in diabetic heart
    A Malhotra
    Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Cardiovasc Res 34:34-40. 1997
    ..Abnormalities in the contractile proteins and regulatory proteins could be responsible for the mechanical defects in streptozotocin (STZ)-diabetic ..
  59. ncbi Mutations in the gene encoding filamin B disrupt vertebral segmentation, joint formation and skeletogenesis
    Deborah Krakow
    Department of Obstetrics and Gynecology, Cedars Sinai Research Institute, and David Geffen School of Medicine at UCLA, Los Angeles, California, USA
    Nat Genet 36:405-10. 2004
    ..These data indicate an unexpected role in vertebral segmentation, joint formation and endochondral ossification for this ubiquitously expressed cytoskeletal protein...
  60. ncbi Cindr interacts with anillin to control cytokinesis in Drosophila melanogaster
    Kaisa Haglund
    Department of Biochemistry, Institute for Cancer Research, Oslo University Hospital, N 0310 Oslo, Norway
    Curr Biol 20:944-50. 2010
    ..We propose that Cindr and Anillin cooperate to promote intercellular bridge stability during incomplete cytokinesis in Drosophila melanogaster...
  61. ncbi Profilin and the Abl tyrosine kinase are required for motor axon outgrowth in the Drosophila embryo
    Z Wills
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Neuron 22:291-9. 1999
    ..Moreover, the phenotype of a double mutant suggests that these components function together to control axonal outgrowth...
  62. pmc The essential role of profilin in the assembly of actin for microspike formation
    S Suetsugu
    Department of Biochemistry, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    EMBO J 17:6516-26. 1998
    ..These findings provide the first evidence that profilin is a key molecule linking a signaling network to rapid actin polymerization in microspike formation...
  63. ncbi Filamin A is required for vimentin-mediated cell adhesion and spreading
    Hugh Kim
    Canadian Institutes of Health Group in Matrix Dynamics, University of Toronto, Toronto, Ontario, M5S 3E2, Canada
    Am J Physiol Cell Physiol 298:C221-36. 2010
    ..These data indicate that filamin A associates with vimentin and to protein kinase C-epsilon, thereby enabling vimentin phosphorylation, which is important for beta1 integrin activation and cell spreading on collagen...
  64. ncbi Characterization of anillin mutants reveals essential roles in septin localization and plasma membrane integrity
    Christine M Field
    Department of Systems Biology, Harvard Medical School, Boston MA 02115, USA
    Development 132:2849-60. 2005
    ..Cumulatively, our data point to an important role for Anillin in scaffolding cleavage furrow components, directly stabilizing intracellular bridges, and indirectly stabilizing newly deposited plasma membrane during cellularization...
  65. pmc Rho-dependent control of anillin behavior during cytokinesis
    Gilles R X Hickson
    Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA
    J Cell Biol 180:285-94. 2008
    ..We propose that such interactions occur dynamically and transiently to promote furrow stability...
  66. ncbi FLNA and PGK1 are two potential markers for progression in hepatocellular carcinoma
    Jianzhong Ai
    Core Facility of Genetically Engineered Mice, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, China
    Cell Physiol Biochem 27:207-16. 2011
    ..Until now, the prognosis for the metastasis of HCC had not improved. Recently, several proteins that are related to metastasis and invasion of HCC were identified, but the effective markers still remain to be elucidated...
  67. ncbi The regulation mechanism for the auto-inhibition of binding of human filamin A to integrin
    Ulla Pentikäinen
    Department of Biological and Environmental Science, University of Jyvaskyla, Jyvaskyla, Finland
    J Mol Biol 393:644-57. 2009
    ..This observed force-induced conformational change can also be one of possible mechanisms involved in the regulation of integrin activation...
  68. ncbi Focal adhesions - the cytoskeletal connection
    D R Critchley
    Department of Biochemistry, University of Leicester, Leicester, LE1 7RH, England
    Curr Opin Cell Biol 12:133-9. 2000
    ....
  69. pmc Cooperative interactions between the central spindle and the contractile ring during Drosophila cytokinesis
    M G Giansanti
    Istituto Pasteur Fondazione Cenci Bolognetti, Dipartimento di Genetica e Biologia Molecolare, Universita di Roma La Sapienza, 00185 Rome, Italy
    Genes Dev 12:396-410. 1998
    ..These observations all indicate that proper actin assembly is necessary for centrosome separation and migration...
  70. ncbi Interaction of the pacemaker channel HCN1 with filamin A
    Biagio Gravante
    Department of Biomolecular Sciences and Biotechnology, Laboratory of Molecular Physiology and Neurobiology, University of Milano, Via Celoria 26, 20133, Italy
    J Biol Chem 279:43847-53. 2004
    ..The interaction with filamin A may contribute to localizing HCN1 channels to specific neuronal areas and to modulating channel activity...
  71. pmc R-Ras regulates migration through an interaction with filamin A in melanoma cells
    Joanna E Gawecka
    Natural Products and Cancer Biology, Cancer Research Center of Hawaii, University of Hawaii at Manoa, Honolulu, Hawaii, United States of America
    PLoS ONE 5:e11269. 2010
    ..Using a yeast two-hybrid approach we sought to identify novel R-Ras binding proteins that might mediate its effects on integrins...
  72. ncbi X-ray structure determination of human profilin II: A comparative structural analysis of human profilins
    I M Nodelman
    Department of Molecular Biology, Henry H Hoyt Laboratory, Princeton University, Princeton, NJ 08544, USA
    J Mol Biol 294:1271-85. 1999
    ..These observations are important for the understanding of the functional and structural differences between these two classes of profilin isoforms...
  73. ncbi Citron kinase mediates transition from constriction to abscission through its coiled-coil domain
    Sadanori Watanabe
    Department of Pharmacology, Kyoto University Faculty of Medicine, Kyoto 606 8501, Japan
    J Cell Sci 126:1773-84. 2013
    ..We propose that the CC-domain-mediated translocation and actions of Citron-K ensure proper stabilization of the midbody structure during the transition from constriction to abscission...
  74. ncbi FilGAP, a Rho- and ROCK-regulated GAP for Rac binds filamin A to control actin remodelling
    Yasutaka Ohta
    Hematology Division, Brigham and Women s Hospital, Department of Medicine, Harvard Medical School, Boston, MA 02115, USA
    Nat Cell Biol 8:803-14. 2006
    ..FilGAP is, therefore, a mediator of the well-established antagonism of Rac by RhoA that suppresses leading edge protrusion and promotes cell retraction to achieve cellular polarity...
  75. ncbi The pathomechanism of filaminopathy: altered biochemical properties explain the cellular phenotype of a protein aggregation myopathy
    Thomas Lowe
    Department of Cell Biology, University of Potsdam, 14476 Potsdam Golm, Germany
    Hum Mol Genet 16:1351-8. 2007
    ..The mutant filamin does not associate with wild type filamin. These findings are of great importance to explain the pathomechanism of this disease...
  76. pmc Filamin a binds to CCR2B and regulates its internalization
    Laura Minsaas
    Department of Biomedicine, University of Bergen, Bergen, Norway
    PLoS ONE 5:e12212. 2010
    ..Therefore, FLNa emerges as an important protein for controlling the internalization and spatial localization of the CCR2B receptor in different dynamic membrane structures...
  77. ncbi Filamin is essential in actin cytoskeletal assembly mediated by p21-activated kinase 1
    Ratna K Vadlamudi
    Department of Molecular and Cellular Oncology, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Nat Cell Biol 4:681-90. 2002
    ....
  78. pmc Macrophage mesenchymal migration requires podosome stabilization by filamin A
    Romain Guiet
    CNRS, Institut de Pharmacologie et de Biologie Structurale IPBS, Unité Mixte de Recherche 5089, 205 route de Narbonne, Toulouse, France
    J Biol Chem 287:13051-62. 2012
    ..These new functions assigned to FLNa, particularly its role in mesenchymal migration, could be directly related to the defects in cell migration described during the embryonic development in FLNa-defective patients...
  79. ncbi Orbit/Mast, the CLASP orthologue of Drosophila, is required for asymmetric stem cell and cystocyte divisions and development of the polarised microtubule network that interconnects oocyte and nurse cells during oogenesis
    Endre Mathe
    Cancer Research UK, Cell Cycle Genetics Research Group, Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EN, UK
    Development 130:901-15. 2003
    ..The localisation of CLIP-190 to such microtubules and to the fusome is dependent upon Orbit/Mast to which it is complexed...
  80. ncbi Determination of a cAMP-dependent protein kinase phosphorylation site in the C-terminal region of human endothelial actin-binding protein
    D Jay
    Departamento de Cultivo de Tejidos, Instituto Nacional de Cardiologia, Ignacio Chavez, Juan Badiano 1, D F Mexico
    Arch Biochem Biophys 377:80-4. 2000
    ..The functional significance of phosphorylation of this residue, which belongs to a serine-proline motif, is discussed...
  81. ncbi Regulation of cell adhesion to collagen via beta1 integrins is dependent on interactions of filamin A with vimentin and protein kinase C epsilon
    Hugh Kim
    CIHR Group in Matrix Dynamics, University of Toronto, Room 239, 150 College Street, Toronto, Ontario, Canada M5S 3E2
    Exp Cell Res 316:1829-44. 2010
    ..We conclude that the binding of filamin A to vimentin and protein kinase Cepsilon is an essential regulatory step for the trafficking and activation of beta1 integrins and cell spreading on collagen...
  82. pmc Identification of Filamin A as a BRCA1-interacting protein required for efficient DNA repair
    Aneliya Velkova
    Risk Assessment, Detection and Intervention Program, H Lee Moffitt Cancer Center, Tampa, FL, USA
    Cell Cycle 9:1421-33. 2010
    ..Our data is consistent with a model in which absence of FLNA compromises homologous recombination and non-homologous end joining. Our findings have implications for the response to irradiation induced DNA damage...
  83. pmc R-Ras interacts with filamin a to maintain endothelial barrier function
    G S Griffiths
    Department of Cell and Molecular Biology, Cardiovascular Research Center and the John A Burns School of Medicine, University of Hawaii at Manoa, Honolulu, Hawaii 96813, USA
    J Cell Physiol 226:2287-96. 2011
    ....
  84. ncbi Disease-associated substitutions in the filamin B actin binding domain confer enhanced actin binding affinity in the absence of major structural disturbance: Insights from the crystal structures of filamin B actin binding domains
    Gregory M Sawyer
    Institute of Molecular Biosciences, Massey University, Palmerston North, New Zealand
    J Mol Biol 390:1030-47. 2009
    ..0 microM. The increased F-actin binding affinity of the mutants presents a biochemical mechanism that differentiates the autosomal dominant gain-of-function FLNB disorders from those that arise through the complete loss of FLNB protein...
  85. pmc Prognostic values of filamin-A status for topoisomerase II poison chemotherapy
    Jingyin Yue
    Department of Radiation Oncology, The Cancer Institute of New Jersey, Robert Wood Johnson Medical School, New Brunswick, NJ 08903, USA
    Int J Biol Sci 8:442-50. 2012
    ..These data suggest a novel function of filamin-A in regulating the pharmacokinetics of Topo II poisons, and that the status of filamin-A may be used as a prognostic marker for Topo II poisons based cancer treatments...
  86. ncbi Profilin promotes barbed-end actin filament assembly without lowering the critical concentration
    F Kang
    Department of Medicine, University of Florida College of Medicine, Gainesville, Florida 32610 0245, USA
    J Biol Chem 274:36963-72. 1999
    ..Our findings indicate that profilin serves as a polymerization catalyst that captures actin monomers from Thymosin-beta4. Actin and ushers actin as a Profilin. Actin complex onto growing barbed filament ends...
  87. ncbi Bilateral periventricular nodular heterotopia in France: frequency of mutations in FLNA, phenotypic heterogeneity and spectrum of mutations
    G Sole
    Laboratoire de Génétique Humaine, Universite Victor Segalen Bordeaux 2, 33076 Bordeaux Cedex, France
    J Neurol Neurosurg Psychiatry 80:1394-8. 2009
    ..Three additional patients with BPNH-EDS and a mutation in FLNA are described. No phenotype-genotype correlations could be established, but these clinical data sustain the importance of cardiovascular monitoring in FLNA-BPNH patients...
  88. pmc Molecular basis of filamin A-FilGAP interaction and its impairment in congenital disorders associated with filamin A mutations
    Fumihiko Nakamura
    Translational Medicine Division, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA, USA
    PLoS ONE 4:e4928. 2009
    ..Mutations in filamin A (FLNa), an essential cytoskeletal protein with multiple binding partners, cause developmental anomalies in humans...
  89. ncbi Differential involvement of sarcomeric proteins in myofibrillar myopathies: a morphological and immunohistochemical study
    Kristl G Claeys
    Institut de Myologie, Groupe Hospitalier Pitie Salpetriere, Paris, France
    Acta Neuropathol 117:293-307. 2009
    ....
  90. ncbi Phosphorylated filamin A regulates actin-linked caveolae dynamics
    Olivia Muriel
    Integrin Signaling Laboratory, Department of Vascular Biology, Centro Nacional de Investigaciones Cardiovasculares CNIC, Melchor Fernandez Almagro 3, 28029 Madrid, Spain
    J Cell Sci 124:2763-76. 2011
    ..Inwards trafficking of caveolin-1 requires both the ability of FLNa to bind actin and cycling PKCα-dependent phosphorylation of FLNa on Ser2152 after cell detachment...
  91. ncbi Actin-binding protein-280 binds the stress-activated protein kinase (SAPK) activator SEK-1 and is required for tumor necrosis factor-alpha activation of SAPK in melanoma cells
    A Marti
    Diabetes Unit and Medical Services, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 021291, USA
    J Biol Chem 272:2620-8. 1997
    ..ABP-280 participates in TNF-alpha signal transduction to SAPKs, in part through the binding of SEK-1...
  92. pmc Atomic structures of two novel immunoglobulin-like domain pairs in the actin cross-linking protein filamin
    Outi K Heikkinen
    Department of Chemistry, University of Helsinki, FI 00014 Helsinki, Finland
    J Biol Chem 284:25450-8. 2009
    ....
  93. pmc Analyzing the effects of delaying aster separation on furrow formation during cytokinesis in the Caenorhabditis elegans embryo
    Lindsay Lewellyn
    Department of Cellular and Molecular Medicine, Ludwig Institute for Cancer Research, University of California, San Diego, La Jolla, CA 92093, USA
    Mol Biol Cell 21:50-62. 2010
    ....
  94. pmc Filamin is required for ring canal assembly and actin organization during Drosophila oogenesis
    M G Li
    Department of Genetics, Cell and Developmental Biology, University of Minnesota, St Paul, Minnesota 55108, USA
    J Cell Biol 146:1061-74. 1999
    ..The genetic and molecular characterization of Drosophila filamin provides the first genetic model system for the analysis of filamin function and regulation during development...
  95. ncbi Acidocalcisomes are functionally linked to the contractile vacuole of Dictyostelium discoideum
    Norma Marchesini
    Laboratory of Molecular Parasitology, Department of Pathobiology, University of Illinois at Urbana Champaign, Urbana, Illinois 61802, USA
    J Biol Chem 277:8146-53. 2002
    ..Together, these results suggest that the mass-dense granules of D. discoideum are homologous to the acidocalcisomes described in protozoan parasites and are linked to the function of the contractile vacuole...
  96. ncbi Spatial control of cytokinesis by Cdr2 kinase and Mid1/anillin nuclear export
    Maria Almonacid
    Institut Curie, Centre de Recherche, F 75248 Paris, France
    Curr Biol 19:961-6. 2009
    ..We conclude that Cdr2- and nuclear export-dependent positioning of Mid1 constitute two overlapping mechanisms that relay cell polarity and nuclear positional information to ensure proper division-plane specification...
  97. ncbi A 90 kDa fragment of filamin A promotes Casodex-induced growth inhibition in Casodex-resistant androgen receptor positive C4-2 prostate cancer cells
    Y Wang
    Department of Urology, School of Medicine, University of California Davis, Sacramento, CA, USA
    Oncogene 26:6061-70. 2007
    ..Our results demonstrate that expression of nuclear FlnA is necessary for androgen dependence in these cells...
  98. ncbi Stepping into the ring: the SIN takes on contractile ring assembly
    Rachel H Roberts-Galbraith
    Howard Hughes Medical Institute, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Genes Dev 22:3082-8. 2008
    ..Furthermore, their careful analysis of contractile ring formation may help bridge two existing models of cytokinetic ring formation...
  99. pmc Filamin-A fragment localizes to the nucleus to regulate androgen receptor and coactivator functions
    C J Loy
    Department of Obstetrics and Gynecology, National University of Singapore, Singapore 119074
    Proc Natl Acad Sci U S A 100:4562-7. 2003
    ..This unanticipated role of FLNa adds to the growing evidence for the involvement of cytoskeletal proteins in transcription regulation...
  100. pmc CD28 and CTLA-4 coreceptor expression and signal transduction
    Christopher E Rudd
    Department of Pathology, Cell Signalling Section, University of Cambridge, Cambridge, UK
    Immunol Rev 229:12-26. 2009
    ....
  101. ncbi Physical and functional interaction of filamin (actin-binding protein-280) and tumor necrosis factor receptor-associated factor 2
    A Leonardi
    Immune Activation Section, Laboratory of Immunoregulation, NIAID, National Institutes of Health, Bethesda, Maryland 20892 1876, USA
    J Biol Chem 275:271-8. 2000
    ..Reintroduction of Filamin into these cells restores the TNF response. The data imply a role for Filamin in inflammatory signal transduction pathways...

Research Grants62

  1. Anthrax Toxins Impair Phagocyte Actin-based Motility
    Frederick Southwick; Fiscal Year: 2010
    ..2. Determine how anthrax toxins directly or indirectly alter the function of one or more specific contractile proteins. A...
  2. Vascular Surgery - Estrogen and the Injury Response
    Richard H Karas; Fiscal Year: 2012
    ..of VSMC proliferation during vascular injury and atherosclerosis;and VSMC ER2 controls genes encoding key contractile proteins regulating VSMC tone...
  3. Impact of AMP-activated kinase on sex differences in hypertrophic cardiomyopathy
    John P Konhilas; Fiscal Year: 2013
    ..cellular energy state, (2) regulating mediators of energy producing pathways, and, (3) directly modifying contractile proteins by phosphorylation...
  4. Pbx Homeodomain Proteins in Skeletal Muscle Differentiation
    LISA A MAVES; Fiscal Year: 2011
    ..skeletal muscle differentiation by sequentially activating groups of genes, including those encoding muscle contractile proteins. The long term goal of this proposal is to elucidate how muscle regulatory factors like Myod activate their ..
  5. C/EBP, atrogin-1, and muscle wasting
    Per Olof Hasselgren; Fiscal Year: 2009
    ..Improved insight into mechanisms accounting for muscle wasting will help develop better treatments of this debilitating condition. ..
  6. Endothelial Lipoxygenase Metabolites and Vascular Tone
    WILLIAM BRYSON CAMPBELL; Fiscal Year: 2013
    ..and angiotensin promote constriction by activating the RhoA-Rho kinase cascade that sensitizes contractile proteins to Ca. 11,12,15-THETA decreases the formation of the active form of RhoA, RhoA-GTP...
  7. Impact of AMP-activated kinase on sex differences in hypertrophic cardiomyopathy
    John P Konhilas; Fiscal Year: 2013
    ..cellular energy state, (2) regulating mediators of energy producing pathways, and, (3) directly modifying contractile proteins by phosphorylation...
  8. Molecular mechanics of mutant cardiac myosin
    Jeffrey R Moore; Fiscal Year: 2013
    ..Our approach measures the mechanical properties of isolated contractile proteins. Therefore, we will determine the direct effects of the FHC mutations on actomyosin...
  9. Regulation of Cardiac Remodeling by SRF-mAKAP complexes
    CATHERINE LAURA PASSARIELLO; Fiscal Year: 2013
    ..regulator of myocyte gene expression, directing the transcription of genes encoding cytoskeletal and contractile proteins, calcium-handling molecules and factors controlling myocyte phenotype...
  10. Regulation of gastrointestinal neuromuscular function by NIBP/NFkB signaling
    Wenhui Hu; Fiscal Year: 2013
    ..cells, (b) nerve-stimulated and agonist-induced GI contraction/relaxation, and (c) expression patterns of contractile proteins and inflammatory mediators in smooth muscles...
  11. Loss of Matrix Regulation and Aortic Aneurysm Development in vivo
    Hiromi Yanagisawa; Fiscal Year: 2013
    ..In addition, ECM has profound effects on smooth muscle cell (SMC) phenotypes and mutations in SMC contractile proteins have been shown to be responsible for subsets of thoracic aortic aneurysms in humans...
  12. Single Cross-Bridge Kinetics in Transgenic Mouse Hearts Expressing FHC Mutations
    Danuta Szczesna-Cordary; Fiscal Year: 2012
    ..FHC) is an autosomal dominant disease originating from mutations in genes that encode for the major contractile proteins of the heart, including the ventricular myosin regulatory (RLC) and essential (ELC) light chains...
  13. Cardiomyocyte stretch and intracellular calcium release with advancing age
    TIMOTHY LEE DOMEIER; Fiscal Year: 2013
    ..will be used with fluorescent calcium indicators (Fluo-4) with reference to the well-defined spacing of contractile proteins (i.e., sarcomere length)...
  14. Analysis of a Novel Regulator of Excitation-Contraction Coupling in Skeletal Musc
    John Y Kuwada; Fiscal Year: 2013
    ..The increase in calcium ions in turn initiates contraction by activating the contractile proteins. EC coupling occurs at triadic junctions of the transverse tubules that are infoldings of the muscle ..
  15. Mutations in Smooth Muscle Contractile Proteins: Pathways to Vascular Diseases
    Dianna M Milewicz; Fiscal Year: 2013
    ..Project 4 will Investigate SMC cellular pathways leading to thoracic aortic disease and SMC proliferation in these same mouse models. ..
  16. Development of Novel Tools for Gene Targeting in Smooth Muscle
    Rosalyn M Adam; Fiscal Year: 2012
    ..Furthermore, current strategies typically rely on promoters encoding SM contractile proteins such as SM-MHC and SM221 to achieve SM-specific targeting of Cre...
  17. Glycoconjugates and Cardiovascular Disease
    Gerald Warren Hart; Fiscal Year: 2013
    ..the roles of the crosstalk between O-GlcNAcylation and phosphorylation on cardiomyocyte mitochondrial and contractile proteins in diabetic cardiomyopathy...
  18. Cerebrovascular Myosin Light Chain Phosphorylation in Fetus, Newborn, and Adult
    William J Pearce; Fiscal Year: 2013
    ..New confocal colocalization methods are capable of resolving interactions among multiple different contractile proteins in these small arteries...
  19. The Effects of Altered Contractility on Cardiac Myocyte Signaling and Hypertrophy
    MARIA LYNN VALENCIK; Fiscal Year: 2012
    ..Our general hypothesis that the forces generated by contractile proteins in cardiac myocytes activate integrin mediated signal transduction leading to the remodeling seen in ..
  20. Oxidative Stress and Myocardial Function
    Marius P Sumandea; Fiscal Year: 2010
    ..The hypothesis underlying this work is that oxidative stress-induced modifications of cardiac contractile proteins are exacerbated with age and cardiac disease progression, and lead to impaired myofilament function...
  21. My-BP-C Modulation of Cardiac Contraction
    Richard L Moss; Fiscal Year: 2013
    ..1) cMyBP-C is the primary regulator of myofibrillar contractile kinetics due to PKA phosphorylation of contractile proteins during )B-adrenergic stimulation of myocardium, 2) cMyBP-C binds to myosin along the long axis of the thick ..
  22. Myosin Binding Protein C structure-function relationships in the failing heart
    Pieter P de Tombe; Fiscal Year: 2010
    ..morbidity is associated with maladaptation of many aspects of cardiac function, including dysfunction of contractile proteins. The purpose of this proposal is to make possible hiring of a new Asst...
  23. A multi-scale approach to airway hyperresponsiveness: from molecule to organ
    MERRYN HOWATSON TAWHAI; Fiscal Year: 2013
    ..We will include in this extended model: 1) at the molecular level, the kinetics of the contractile proteins during regular cross-bridge cycling and during the latch-state and their contributions to force production, ..
  24. MECHANISM OF MUSCLE WASTING IN AGING MAN
    K Sreekumaran Nair; Fiscal Year: 2013
    ..old proteins with newly synthesized proteins whereas resistance exercise increases synthesis of specific contractile proteins with effect on replacing old and modified proteins...
  25. Identifying the Genetic and Molecular Basis of Clubfoot
    Anita E Beck; Fiscal Year: 2013
    Tlie overall goal of this project is to understand liow mutations in genes that encode contractile proteins cause clubfoot in a group of disorders called distal arthrogryposis (DA) syndromes and test whether variants in these genes ..
  26. Myocardial Protection during Fetal Bypass: Role of Calcium Cycling
    Charles R Cole; Fiscal Year: 2011
    ..The central hypothesis of this proposal is that disruption of intracellular calcium cycling and contractile proteins contribute to cardiac dysfunction associated with fetal cardiac bypass and that modified cardioplegia can ..
  27. MYOCARDIAL PROTEIN SYNTHESIS AFTER ALCOHOL INTOXICATION
    Charles H Lang; Fiscal Year: 2010
    ..The net effect is manifested through alterations in the expression of myocardial proteins including contractile proteins. We further hypothesize that provision of amino acids either through acute gavage or meal feeding to rats ..
  28. LENGTH REGULATION OF CARDIAC CONTRACTION
    Donald Martyn; Fiscal Year: 2010
    ..Physiologically, cardiac force sensitivity to [Ca2+] is regulated by altered phosphorylation of contractile proteins, including TF regulatory subunits, myosin regulatory light chains, myosin-binding protein-C and titin...
  29. Obscurin & Myofibrils in Cardiac & Skeletal Muscle
    Robert Bloch; Fiscal Year: 2009
    ..The results of our experiments should reveal some of the key mechanisms involved in assembling contractile proteins into sarcomeres and in determining their association with the SR.
  30. Role of Thrombospondin-4 in Cardiac Mechanotransduction
    Oscar Cingolani; Fiscal Year: 2013
    ..link between TSP4 and stretch-mediated cardiac myocyte increases in calcium, augmented sensitivity of contractile proteins to calcium or both;and determine the source of calcium affected by the lack of TSP4...
  31. Regulation of vascular smooth muscle calcium sensitivity
    Paul H Ratz; Fiscal Year: 2010
    ..which in turn, is regulated by changes in the levels of cytosolic free calcium (Ca) and the sensitivity of contractile proteins to Ca. This project will focus on mechanisms regulating rhoA kinase (ROK)-dependent Ca sensitivity...
  32. STRUCTURAL DYNAMICS OF ACTOMYOSIN MOTILITY
    Yale E Goldman; Fiscal Year: 2013
    ..applied to non-muscle myosin molecular motors to probe the relations between biochemical reactions of the contractile proteins, the elementary mechanical steps of the cross-bridge cycle and the corresponding structural motions...
  33. Ionic Mechanisms of Stretch-Activation in Muscular Lymphatics
    DAVID CARL ZAWIEJA; Fiscal Year: 2012
    ..Lymphatic muscle utilizes a unique combination of tonic and phasic contractions and muscle contractile proteins to generate and control lymph flow/transport...
  34. Thrombospondin-1 Regulates Vascular Tone
    Jeff S Isenberg; Fiscal Year: 2010
    ..In support of this hypothesis we propose three specific aims: 1) Demonstrate the effect TSP1 has upon contractile proteins in vascular smooth muscle cells. 2) Demonstrate the effects TSP1 has upon NO-driven vasorelaxation of VSMC...
  35. CYTOSKELETAL PROTEINS IN RETINAL PHOTORECEPTORS
    MICHAEL CHAITIN; Fiscal Year: 1991
    ..This will help us to determine whether the generating outer segments undergo a disorganized breakdown, or if the degeneration follows a methodical sequence of events indicative of a "de-differentiation" of the visual cells...
  36. CELL NA IN VSM PROTEIN PRODUCTION
    JULIUS ALLEN; Fiscal Year: 1992
    The basic underlying hypothesis of this proposal is that (Na+)i plays a role in regulating the amount of contractile proteins and the number of Na+ pump sites in cultured vascular smooth muscle cells...
  37. PROTEIN PHOSPHATASES IN ENDOTHELIAL CELL PERMEABILITY
    JOE GARCIA; Fiscal Year: 2001
    ..b>Contractile proteins, actin and myosin, control permeability and bioactive agonists such as thrombin induce endothelial cell ..
  38. THE ROLE OF CONTRACTILE PROTEINS IN ERYTHROCYTE AGING
    DIANE LIN; Fiscal Year: 1980
    ..The biochemical approach will be on the characterization of the contractile proteins in the intact cell and in isolated form, including studies on the degree of phosphorylation, state of ..
  39. Structure & function of cardiac contractile proteins
    Jeffrey Robbins; Fiscal Year: 2004
    ..Together with the models above, it will provide a comprehensive picture of the alpha-MHC's effects on the development of hypertrophy, dilation and failure. ..
  40. THE ROLE OF CONTRACTILE PROTEINS IN PLATELETS
    ELENA PUSZKIN; Fiscal Year: 1990
    ..These studies may clarify the complex interactions that occur between the membrane of platelets with the internal cytoskeleton that are active in supporting platelet function during aggregation and/or thrombus formation...
  41. BIOCHEMISTRY OF CONTRACTILE PROTEINS
    David Hartshorne; Fiscal Year: 2009
    ..If successful, they will establish an understanding at a molecular level of a process essential for function in muscle and non-muscle cells, and will facilitate pharmacological intervention. ..
  42. REGULATION OF CONTRACTILE PROTEINS IN HUMAN MYOCARDIUM
    Ronglih Liao; Fiscal Year: 2000
    ....
  43. FUNCTIONAL DOMAINS IN CARDIAC TROPONIN C
    JOHN PUTKEY; Fiscal Year: 1992
    ..of the Ca2+ signal and transmission of this information through a cascade of myofibril regulatory and contractile proteins. The critical role for troponin C in regulation of muscle contraction emphasizes a need to fully understand ..
  44. DEVELOPMENTAL ALTERATIONS IN CARDIAC PROTEIN TURNOVER
    Allen Samarel; Fiscal Year: 2003
    ..data indicate that focal adhesion/costmere assembly is critical to the assembly of newly synthesized contractile proteins into sarcomeres...
  45. REGULATORY ELEMENTS CONTROLLING TROPONIN I EXPRESSION
    STEPHEN KONIECZNY; Fiscal Year: 1993
    ..from the cell cycle, the formation of multinucleated muscle fibers and the coordinate accumulation of the contractile proteins including alpha-actin, myosin heavy chain, myosin light chain, alpha-tropomyosin, troponins C,I and T, and ..
  46. GORDON RESEARCH CONFERENCE--MUSCLE CONTRACTILE PROTEINS
    PETER VIBERT; Fiscal Year: 1993
    The proposed conference on Contractile Proteins will be the 19th in a series of Gordon Research Conferences that originated with a conference on cardiac muscle in 1966...
  47. Bioengineering Analysis of Muscle Mechanics & Metabolism
    SRBOLJUB MIJAILOVICH; Fiscal Year: 2007
    ..model that can be used to (1) generate new mechanistic hypotheses concerning the functions of the contractile proteins myosin and actin and (2) quantitatively evaluate the roles of accessory and regulatory proteins in ..
  48. GENE EXPRESSION DURING DROSOPHILA MYOGENESIS
    Allen Shearn; Fiscal Year: 1999
    ..This work advanced our understanding of contractile proteins, illuminated fundamental aspects of myofibril assembly and function, and provided insights into the ..
  49. MECHANISM OF CARDIAC MUSCLE CONTRACTION
    ROBERT BARSOTTI; Fiscal Year: 1999
    ..characterizes the elementary steps of the actin-myosin ATPase cycle in skeletal muscle using both isolated contractile proteins and muscle fibers...
  50. CYTOKINESIS IN DICTYOSTELIUM--A MOLECULAR ANALYSIS
    James Spudich; Fiscal Year: 2001
    ..While this process of cytokinesis clearly involves the contractile proteins actin and myosin, which accumulate in the furrow as a contractile ring, virtually nothing is understood at ..
  51. MUSCLE CHEMOMECHANICAL TRANSDUCTION AND ITS REGULATION
    Earl Homsher; Fiscal Year: 2007
    ..of shortening, and calcium sensitivity which are the ultimate expressions of the interaction between the contractile proteins myosin and actin Point, missense, and deletion mutations in troponin T and tropomyosin produce hypertrophic ..
  52. CONTRACTILE PROTEIN EXPRESSION IN FAILING HUMAN HEART
    Michael Bristow; Fiscal Year: 2002
    ..b>Contractile proteins are one of the primary means of regulating contractile function, because they possess or regulate the ..
  53. GROWTH AND DIFFERENTIATION OF HUMAN CARDIOBLASTS
    DONALD KOHTZ; Fiscal Year: 1992
    ..In addition, variability occurs in the developmental expression of contractile proteins in the hearts of different mammalian species, making it difficult to determine the developmental program of ..
  54. REGULATING MYOCARDIAL FATTY ACID OXIDATION IN DIABETES
    GEORGE COOK; Fiscal Year: 2003
    ..altered expression patterns of different isoforms of many proteins including enzymes of metabolism and the contractile proteins. Fatty acid oxidation inhibitors [especially carnitine palmitoyltransferase-I (CPT-I) inhibitors] increase ..
  55. STRUCTURE, FUNCTION & MECHANISM OF CONTRACTILE PROTEINS
    YALE GOLDMAN; Fiscal Year: 2002
    ..the Program are the structural biology, mechano-chemistry, protein design and sequence specificity of the contractile proteins. It integrates investigations from genetic expression, isoform diversity, enzymatic activity, molecular ..
  56. SRF/MYOCD: new targets in Alzheimer's neurovasculature
    JOSEPH MIANO; Fiscal Year: 2006
    ..Consequently, AD-VSMC display elevated expression of several contractile proteins that effect a hypercontractile state in these cells...
  57. ATHEROSCLEROSIS AND THE ARTERIAL CONTRACTILE PROTEINS
    DIXIE FREDERIKSEN; Fiscal Year: 1980
    ..b>Contractile proteins from proliferating atherosclerotic lesions will be studied to determine the nature of molecular changes in ..
  58. ALTERNATIVE SPLICING OF A POTASSIUM CHANNEL GENE
    Linda Iverson; Fiscal Year: 2000
    ..common in excitable cells such as nerve and muscle where many of the genes encoding ion channels and muscle contractile proteins show extremely complex tissue-and cell-type specific splicing patterns...
  59. Workshop on Multi-Scale Muscle Mechanics
    BRUCE DAMON; Fiscal Year: 2009
    ..that originate at widely ranging spatial scales, beginning with the structures and behaviors of the contractile proteins and extending through to the manner in which multiple muscles and joints interact during normal and ..
  60. Gordon Research Conference: Muscle: Contractile Proteins
    David Warshaw; Fiscal Year: 2002
    Partial support is requested for the 2002 Gordon Research Conference Muscle: Contractile Proteins to bring together biophysicists, biochemists, cell biologists and physiologists to address the mechanism of muscle contraction and its ..
  61. CONTRACTILE FORCE AND CALCIUM HANDLING IN HEART FAILURE
    Pieter de Tombe; Fiscal Year: 2000
    ..Alterations in the content and isoform distribution of contractile proteins and key proteins involved in calcium handling have been found in CHF...
  62. REGULATION OF SMOOTH MUSCLE ACTOMYOSIN BY CALPONIN
    JOE HAEBERLE; Fiscal Year: 2003
    ..part of a larger effort to understand how the contraction of smooth muscle is regulated at the level of contractile proteins, actin and myosin...