contractile proteins

Summary

Summary: Proteins which participate in contractile processes. They include MUSCLE PROTEINS as well as those found in other cells and tissues. In the latter, these proteins participate in localized contractile events in the cytoplasm, in motile activity, and in cell aggregation phenomena.

Top Publications

  1. ncbi Mena, a relative of VASP and Drosophila Enabled, is implicated in the control of microfilament dynamics
    F B Gertler
    Division of Molecular Medicine, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA
    Cell 87:227-39. 1996
  2. pmc Identification of filamin as a novel ligand for caveolin-1: evidence for the organization of caveolin-1-associated membrane domains by the actin cytoskeleton
    M Stahlhut
    Structural Cell Biology Unit, Department of Medical Anatomy, The Panum Institute, University of Copenhagen, DK 2200 Copenhagen N, Denmark
    Mol Biol Cell 11:325-37. 2000
  3. ncbi Self- and actin-templated assembly of Mammalian septins
    Makoto Kinoshita
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Dev Cell 3:791-802. 2002
  4. pmc Filamin A (FLNA) is required for cell-cell contact in vascular development and cardiac morphogenesis
    Yuanyi Feng
    Division of Genetics and Department of Cardiology, Children s Hospital Boston, Boston, MA 02215, USA
    Proc Natl Acad Sci U S A 103:19836-41. 2006
  5. ncbi Direct observation of dendritic actin filament networks nucleated by Arp2/3 complex and WASP/Scar proteins
    L Blanchoin
    Structural Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037, USA
    Nature 404:1007-11. 2000
  6. pmc Filamin A regulates caveolae internalization and trafficking in endothelial cells
    Maria Sverdlov
    Department of Pharmacology, University of Illinois at Chicago, Chicago, IL 60612, USA
    Mol Biol Cell 20:4531-40. 2009
  7. ncbi Structural and functional aspects of filamins
    A van Der Flier
    Division of Cell Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands
    Biochim Biophys Acta 1538:99-117. 2001
  8. ncbi Molecular mechanisms controlling actin filament dynamics in nonmuscle cells
    T D Pollard
    Structural Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037, USA
    Annu Rev Biophys Biomol Struct 29:545-76. 2000
  9. pmc The filamins: organizers of cell structure and function
    Fumihiko Nakamura
    Translational Medicine Division, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA, USA
    Cell Adh Migr 5:160-9. 2011
  10. pmc Filamin B plays a key role in vascular endothelial growth factor-induced endothelial cell motility through its interaction with Rac-1 and Vav-2
    Beatriz Del Valle-Pérez
    From the Unitat de Bioquímica i Biologia Molecular, Departament de Ciències Fisiològiques II, Universitat de Barcelona IDIBELL, E 08907 Barcelona, Spain
    J Biol Chem 285:10748-60. 2010

Detail Information

Publications300 found, 100 shown here

  1. ncbi Mena, a relative of VASP and Drosophila Enabled, is implicated in the control of microfilament dynamics
    F B Gertler
    Division of Molecular Medicine, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA
    Cell 87:227-39. 1996
    ..Expression of neural-enriched isoforms of Mena in fibroblasts induces the formation of abnormal F-actin-rich outgrowths, supporting a role for this protein in microfilament assembly and cell motility...
  2. pmc Identification of filamin as a novel ligand for caveolin-1: evidence for the organization of caveolin-1-associated membrane domains by the actin cytoskeleton
    M Stahlhut
    Structural Cell Biology Unit, Department of Medical Anatomy, The Panum Institute, University of Copenhagen, DK 2200 Copenhagen N, Denmark
    Mol Biol Cell 11:325-37. 2000
    ..The actin network, therefore, seems to be directly involved in the spatial organization of caveolin-1-associated membrane domains...
  3. ncbi Self- and actin-templated assembly of Mammalian septins
    Makoto Kinoshita
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Dev Cell 3:791-802. 2002
    ..We conclude that septins alone self-assemble into rings, that adaptor proteins recruit septins to actin bundles, and that septins help organize these bundles...
  4. pmc Filamin A (FLNA) is required for cell-cell contact in vascular development and cardiac morphogenesis
    Yuanyi Feng
    Division of Genetics and Department of Cardiology, Children s Hospital Boston, Boston, MA 02215, USA
    Proc Natl Acad Sci U S A 103:19836-41. 2006
    ..Essential roles for FLNA in intercellular junctions provide a mechanism for the diverse developmental defects seen in patients with FLNA mutations...
  5. ncbi Direct observation of dendritic actin filament networks nucleated by Arp2/3 complex and WASP/Scar proteins
    L Blanchoin
    Structural Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037, USA
    Nature 404:1007-11. 2000
    ..These properties result in the automatic assembly of the branched actin network after activation by proteins of the WASP/Scar family and favour the selective disassembly of proximal regions of the network...
  6. pmc Filamin A regulates caveolae internalization and trafficking in endothelial cells
    Maria Sverdlov
    Department of Pharmacology, University of Illinois at Chicago, Chicago, IL 60612, USA
    Mol Biol Cell 20:4531-40. 2009
    ..Taken together, these data suggest that filamin A association with caveolin-1 promotes caveolae-mediated transport by regulating vesicle internalization, clustering, and trafficking...
  7. ncbi Structural and functional aspects of filamins
    A van Der Flier
    Division of Cell Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands
    Biochim Biophys Acta 1538:99-117. 2001
    ..In this review aspects of filamin structure, its splice variants, binding partners and biological function will be discussed...
  8. ncbi Molecular mechanisms controlling actin filament dynamics in nonmuscle cells
    T D Pollard
    Structural Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037, USA
    Annu Rev Biophys Biomol Struct 29:545-76. 2000
    ..Profilin catalyzes exchange of ADP for ATP, recycling actin back to a pool of unpolymerized monomers bound to profilin and thymosin-beta 4 that is poised for rapid elongation of new barbed ends...
  9. pmc The filamins: organizers of cell structure and function
    Fumihiko Nakamura
    Translational Medicine Division, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA, USA
    Cell Adh Migr 5:160-9. 2011
    ..Due to this diversity, mutations in human FLN genes result in a wide range of anomalies with moderate to lethal consequences. This review focuses on the structure and functions of FLNa in cell migration and adhesion...
  10. pmc Filamin B plays a key role in vascular endothelial growth factor-induced endothelial cell motility through its interaction with Rac-1 and Vav-2
    Beatriz Del Valle-Pérez
    From the Unitat de Bioquímica i Biologia Molecular, Departament de Ciències Fisiològiques II, Universitat de Barcelona IDIBELL, E 08907 Barcelona, Spain
    J Biol Chem 285:10748-60. 2010
    ..In conclusion, our results reveal a crucial role for FLNB in endothelial cell migration and in the angiogenic process in adult endothelial cells...
  11. pmc Exportin 6: a novel nuclear export receptor that is specific for profilin.actin complexes
    Theis Stüven
    ZMBH, INF 282, D 69120 Heidelberg and Universität Lübeck, Ratzeburger Allee 160, D 23538 Lubeck, Germany
    EMBO J 22:5928-40. 2003
    ..In contrast to a previous report, we found no indications of a major and direct role for CRM1 in actin export from mammalian or insect nuclei...
  12. ncbi Recovery from DNA damage-induced G2 arrest requires actin-binding protein filamin-A/actin-binding protein 280
    Xiangbing Meng
    Department of Molecular Genetics and Microbiology, MSC08 4660, 1 University of New Mexico School of Medicine, Albuquerque, New Mexico 87131 0001, USA
    J Biol Chem 279:6098-105. 2004
    ..Together, these data suggest a functional role of filamin-A in the recovery from G2 arrest and subsequent mitotic cell death after DNA damage...
  13. doi FLNA and PGK1 are two potential markers for progression in hepatocellular carcinoma
    Jianzhong Ai
    Core Facility of Genetically Engineered Mice, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, China
    Cell Physiol Biochem 27:207-16. 2011
    ..Until now, the prognosis for the metastasis of HCC had not improved. Recently, several proteins that are related to metastasis and invasion of HCC were identified, but the effective markers still remain to be elucidated...
  14. pmc Filamin-A as a marker and target for DNA damage based cancer therapy
    Jingyin Yue
    Department of Radiation Oncology, The Cancer Institute of New Jersey, UMDNJ Robert Wood Johnson Medical School, 195 Little Albany St, New Brunswick, NJ 08903, USA
    DNA Repair (Amst) 11:192-200. 2012
    ..Thus filamin-A can be used as a biomarker and a target for DNA damage based cancer therapy...
  15. ncbi Migfilin and Mig-2 link focal adhesions to filamin and the actin cytoskeleton and function in cell shape modulation
    Yizeng Tu
    Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Cell 113:37-47. 2003
    ..Together, Mig-2, migfilin and filamin define a connection between cell matrix adhesions and the actin cytoskeleton and participate in the orchestration of actin assembly and cell shape modulation...
  16. pmc Reassembly of contractile actin cortex in cell blebs
    Guillaume T Charras
    Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA
    J Cell Biol 175:477-90. 2006
    ..ezrin was recruited to the membrane first, followed by actin, actin-bundling proteins, and, finally, contractile proteins. Complete assembly of the contractile cortex, which was organized into a cagelike mesh of filaments, took ..
  17. ncbi Bni1p, a yeast formin linking cdc42p and the actin cytoskeleton during polarized morphogenesis
    M Evangelista
    Institute of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia V5A 1S6, Canada
    Science 276:118-22. 1997
    ..Both Bni1p and Bud6p (like Cdc42p and actin) localized to the tips of mating projections. Bni1p may function as a Cdc42p target that links the pheromone response pathway to the actin cytoskeleton...
  18. ncbi Increased Ca2+-sensitivity of the contractile apparatus in end-stage human heart failure results from altered phosphorylation of contractile proteins
    J van der Velden
    Laboratory for Physiology, Institute for Cardiovascular Research ICaR VU, VU University Medical Center, Van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands
    Cardiovasc Res 57:37-47. 2003
    The alterations in contractile proteins underlying enhanced Ca(2+)-sensitivity of the contractile apparatus in end-stage failing human myocardium are still not resolved...
  19. pmc Mid1p/anillin and the septation initiation network orchestrate contractile ring assembly for cytokinesis
    Olivier Hachet
    Cell Cycle Control Laboratory, Swiss Institute for Experimental Cancer Research ISREC, School of Life Sciences, Ecole Polytechnique Federale de Lausanne EPFL, 1066 Epalinges s Lausanne, Switzerland
    Genes Dev 22:3205-16. 2008
    ..Taken together, these data establish that Mid1p and the SIN are the key regulators that orchestrate CAR assembly...
  20. doi Proteomic analysis in aortic media of patients with Marfan syndrome reveals increased activity of calpain 2 in aortic aneurysms
    Christiane Pilop
    Departments of Nephrology and Hypertension, University Hospital of Bern, Bern, Switzerland
    Circulation 120:983-91. 2009
    ..The most life-threatening manifestations are aortic aneurysm and dissection. We investigated changes in the proteome of aortic media in patients with and without MFS to gain insight into molecular mechanisms leading to aortic dilatation...
  21. pmc The small GTPase RalA targets filamin to induce filopodia
    Y Ohta
    Division of Biochemistry and Cellular Biology, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4 1 1 Ogawahigashi, Kodaira, Tokyo 187, Japan
    Proc Natl Acad Sci U S A 96:2122-8. 1999
    ..RalA therefore is a downstream intermediate in Cdc42-mediated filopod production and uses filamin in this pathway...
  22. pmc Disruption of neural progenitors along the ventricular and subventricular zones in periventricular heterotopia
    Russell J Ferland
    Department of Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA
    Hum Mol Genet 18:497-516. 2009
    ..Our current findings suggest that PH formation arises from a final common pathway involving disruption of vesicle trafficking, leading to impaired cell adhesion and loss of neuroependymal integrity...
  23. pmc Nuclear versus cytoplasmic localization of filamin A in prostate cancer: immunohistochemical correlation with metastases
    Roble G Bedolla
    University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA
    Clin Cancer Res 15:788-96. 2009
    ..We have examined whether FlnA localization determines a propensity to metastasis in advanced androgen-independent prostate cancer...
  24. pmc The cytoskeleton protein filamin-A is required for an efficient recombinational DNA double strand break repair
    Jingyin Yue
    Department of Radiation Oncology, The Cancer Institute of New Jersey, University of Medicine and Dentistry of New Jersey Robert Wood Johnson Medical School, New Brunswick, New Jersey 08903, USA
    Cancer Res 69:7978-85. 2009
    ....
  25. pmc Role of proteins of the Ena/VASP family in actin-based motility of Listeria monocytogenes
    V Laurent
    Dynamique du Cytosquelette, Laboratoire d Enzymologie et Biochimie Structurales, CNRS, 91198 Gif sur Yvette, France
    J Cell Biol 144:1245-58. 1999
    ..We propose a molecular ratchet model for insertional polymerization of actin, within which frequent attachment-detachment of VASP to F-actin allows its sliding along the growing filament...
  26. pmc R-Ras regulates migration through an interaction with filamin A in melanoma cells
    Joanna E Gawecka
    Natural Products and Cancer Biology, Cancer Research Center of Hawaii, University of Hawaii at Manoa, Honolulu, Hawaii, United States of America
    PLoS ONE 5:e11269. 2010
    ..Using a yeast two-hybrid approach we sought to identify novel R-Ras binding proteins that might mediate its effects on integrins...
  27. ncbi The Rac1- and RhoG-specific GEF domain of Trio targets filamin to remodel cytoskeletal actin
    J M Bellanger
    CRBM CNRS, UPR 1086, 1919 Route de Mende, 34293 Montpellier Cedex 5, France
    Nat Cell Biol 2:888-92. 2000
    ..In addition, Trio GEFD1 exchange activity is not affected by filamin binding. Our results indicate that filamin, as a molecular target of Trio, may be a scaffold for the spatial organization of Rho-GTPase-mediated signalling pathways...
  28. pmc Androgen-induced cell migration: role of androgen receptor/filamin A association
    Gabriella Castoria
    Dipartimento di Patologia Generale, II Universita di Napoli, Napoli, Italy
    PLoS ONE 6:e17218. 2011
    ..These findings support a role for AR in cell migration and invasiveness. However, the molecular mechanism involved in AR-mediated cell migration still remains elusive...
  29. pmc Filamin A regulates focal adhesion disassembly and suppresses breast cancer cell migration and invasion
    Yingjie Xu
    Lady Davis Institute for Medical Research, Jewish General Hospital, Faculty of Medicine, McGill University, Montreal, Quebec H3T 1E2, Canada
    J Exp Med 207:2421-37. 2010
    ..These results document a regulation of FA dynamics by FLNa in breast cancer cells...
  30. ncbi Mutations in filamin 1 prevent migration of cerebral cortical neurons in human periventricular heterotopia
    J W Fox
    Department of Neurology, Beth Israel Deaconess Medical Center, Harvard Institutes of Medicine, Boston, Massachusetts 02115, USA
    Neuron 21:1315-25. 1998
    ..FLN1 shows previously unrecognized, high-level expression in the developing cortex, is required for neuronal migration to the cortex, and is essential for embryogenesis...
  31. doi Filamins in cell signaling, transcription and organ development
    Alex Xianghua Zhou
    Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, University of Gothenburg, SE 405 30 Goteborg, Sweden
    Trends Cell Biol 20:113-23. 2010
    ..This review focuses on the emerging roles of filamins in cell signaling and transcription, with emphasis on cell motility and organ development...
  32. ncbi Drosophila filamin encoded by the cheerio locus is a component of ovarian ring canals
    N S Sokol
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06510, USA
    Curr Biol 9:1221-30. 1999
    ..Female sterile cheerio mutants fail to recruit actin to ring canals, disrupting the flow of cytoplasm to oocytes...
  33. ncbi Dissecting temporal and spatial control of cytokinesis with a myosin II Inhibitor
    Aaron F Straight
    Department of Cell Biology and Institute of Chemistry and Cell Biology, Harvard Medical School, 250 Longwood Avenue, Boston, MA 02115, USA
    Science 299:1743-7. 2003
    ..Continuous signals from microtubules are required to maintain the position of the cleavage furrow, and these signals control the localization of myosin II independently of other furrow components...
  34. pmc Human endothelial actin-binding protein (ABP-280, nonmuscle filamin): a molecular leaf spring
    J B Gorlin
    Department of Medicine, Massachusetts General Hospital, Charlestown
    J Cell Biol 111:1089-105. 1990
    ..The large size of the leaves, their interruption by two hinges and flexible actin-binding site, facilitate cross-linking of widely dispersed actin filaments...
  35. pmc Activation of the CDC42 effector N-WASP by the Shigella flexneri IcsA protein promotes actin nucleation by Arp2/3 complex and bacterial actin-based motility
    C Egile
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Cell Biol 146:1319-32. 1999
    ..VASP is not involved in Shigella movement, and the function of profilin does not require its binding to proline-rich regions...
  36. pmc Absence of filamin A prevents cells from responding to stiffness gradients on gels coated with collagen but not fibronectin
    Fitzroy J Byfield
    Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Biophys J 96:5095-102. 2009
    ..These results have implications for different models of cell protrusion and adhesion and identify a key role for filamin A in altering cellular stiffness that cannot be compensated for by other actin cross-linkers in vivo...
  37. pmc In mouse brain profilin I and profilin II associate with regulators of the endocytic pathway and actin assembly
    W Witke
    Mouse Biology Programme, EMBL, Monterotondo Rome, Italy
    EMBO J 17:967-76. 1998
    ..Our findings strongly suggest that in brain profilin I and profilin II complexes link the actin cytoskeleton and endocytic membrane flow, directing actin and clathrin assembly to distinct membrane domains...
  38. doi Studies of focal adhesion assembly
    Iain D Campbell
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
    Biochem Soc Trans 36:263-6. 2008
    ....
  39. ncbi Mutations in the X-linked filamin 1 gene cause periventricular nodular heterotopia in males as well as in females
    V L Sheen
    Department of Neurology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02115, USA
    Hum Mol Genet 10:1775-83. 2001
    ..These results demonstrate the sensitivity and specificity of DNA testing for FLN1 mutations and have important functional implications for models of FLN1 protein function in neuronal migration...
  40. pmc Prognostic values of filamin-A status for topoisomerase II poison chemotherapy
    Jingyin Yue
    Department of Radiation Oncology, The Cancer Institute of New Jersey, Robert Wood Johnson Medical School, New Brunswick, NJ 08903, USA
    Int J Biol Sci 8:442-50. 2012
    ..These data suggest a novel function of filamin-A in regulating the pharmacokinetics of Topo II poisons, and that the status of filamin-A may be used as a prognostic marker for Topo II poisons based cancer treatments...
  41. ncbi Determination of a cAMP-dependent protein kinase phosphorylation site in the C-terminal region of human endothelial actin-binding protein
    D Jay
    Departamento de Cultivo de Tejidos, Instituto Nacional de Cardiologia, Ignacio Chavez, Juan Badiano 1, D F Mexico
    Arch Biochem Biophys 377:80-4. 2000
    ..The functional significance of phosphorylation of this residue, which belongs to a serine-proline motif, is discussed...
  42. ncbi Physical and functional interaction of filamin (actin-binding protein-280) and tumor necrosis factor receptor-associated factor 2
    A Leonardi
    Immune Activation Section, Laboratory of Immunoregulation, NIAID, National Institutes of Health, Bethesda, Maryland 20892 1876, USA
    J Biol Chem 275:271-8. 2000
    ..Reintroduction of Filamin into these cells restores the TNF response. The data imply a role for Filamin in inflammatory signal transduction pathways...
  43. ncbi The pathomechanism of filaminopathy: altered biochemical properties explain the cellular phenotype of a protein aggregation myopathy
    Thomas Lowe
    Department of Cell Biology, University of Potsdam, 14476 Potsdam Golm, Germany
    Hum Mol Genet 16:1351-8. 2007
    ..The mutant filamin does not associate with wild type filamin. These findings are of great importance to explain the pathomechanism of this disease...
  44. ncbi FilGAP, a Rho- and ROCK-regulated GAP for Rac binds filamin A to control actin remodelling
    Yasutaka Ohta
    Hematology Division, Brigham and Women s Hospital, Department of Medicine, Harvard Medical School, Boston, MA 02115, USA
    Nat Cell Biol 8:803-14. 2006
    ..FilGAP is, therefore, a mediator of the well-established antagonism of Rac by RhoA that suppresses leading edge protrusion and promotes cell retraction to achieve cellular polarity...
  45. pmc Identification of Filamin A as a BRCA1-interacting protein required for efficient DNA repair
    Aneliya Velkova
    Risk Assessment, Detection and Intervention Program, H Lee Moffitt Cancer Center, Tampa, FL, USA
    Cell Cycle 9:1421-33. 2010
    ..Our data is consistent with a model in which absence of FLNA compromises homologous recombination and non-homologous end joining. Our findings have implications for the response to irradiation induced DNA damage...
  46. ncbi Orbit/Mast, the CLASP orthologue of Drosophila, is required for asymmetric stem cell and cystocyte divisions and development of the polarised microtubule network that interconnects oocyte and nurse cells during oogenesis
    Endre Mathe
    Cancer Research UK, Cell Cycle Genetics Research Group, Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EN, UK
    Development 130:901-15. 2003
    ..The localisation of CLIP-190 to such microtubules and to the fusome is dependent upon Orbit/Mast to which it is complexed...
  47. ncbi Regulation of cell adhesion to collagen via beta1 integrins is dependent on interactions of filamin A with vimentin and protein kinase C epsilon
    Hugh Kim
    CIHR Group in Matrix Dynamics, University of Toronto, Room 239, 150 College Street, Toronto, Ontario, Canada M5S 3E2
    Exp Cell Res 316:1829-44. 2010
    ..We conclude that the binding of filamin A to vimentin and protein kinase Cepsilon is an essential regulatory step for the trafficking and activation of beta1 integrins and cell spreading on collagen...
  48. pmc Filamin a binds to CCR2B and regulates its internalization
    Laura Minsaas
    Department of Biomedicine, University of Bergen, Bergen, Norway
    PLoS ONE 5:e12212. 2010
    ..Therefore, FLNa emerges as an important protein for controlling the internalization and spatial localization of the CCR2B receptor in different dynamic membrane structures...
  49. pmc Macrophage mesenchymal migration requires podosome stabilization by filamin A
    Romain Guiet
    CNRS, Institut de Pharmacologie et de Biologie Structurale IPBS, Unité Mixte de Recherche 5089, 205 route de Narbonne, Toulouse, France
    J Biol Chem 287:13051-62. 2012
    ..These new functions assigned to FLNa, particularly its role in mesenchymal migration, could be directly related to the defects in cell migration described during the embryonic development in FLNa-defective patients...
  50. doi Disease-associated substitutions in the filamin B actin binding domain confer enhanced actin binding affinity in the absence of major structural disturbance: Insights from the crystal structures of filamin B actin binding domains
    Gregory M Sawyer
    Institute of Molecular Biosciences, Massey University, Palmerston North, New Zealand
    J Mol Biol 390:1030-47. 2009
    ..0 microM. The increased F-actin binding affinity of the mutants presents a biochemical mechanism that differentiates the autosomal dominant gain-of-function FLNB disorders from those that arise through the complete loss of FLNB protein...
  51. ncbi Filamin is essential in actin cytoskeletal assembly mediated by p21-activated kinase 1
    Ratna K Vadlamudi
    Department of Molecular and Cellular Oncology, The University of Texas M D Anderson Cancer Center, Houston, Texas 77030, USA
    Nat Cell Biol 4:681-90. 2002
    ....
  52. pmc R-Ras interacts with filamin a to maintain endothelial barrier function
    G S Griffiths
    Department of Cell and Molecular Biology, Cardiovascular Research Center and the John A Burns School of Medicine, University of Hawaii at Manoa, Honolulu, Hawaii 96813, USA
    J Cell Physiol 226:2287-96. 2011
    ....
  53. pmc Atomic structures of two novel immunoglobulin-like domain pairs in the actin cross-linking protein filamin
    Outi K Heikkinen
    Department of Chemistry, University of Helsinki, FI 00014 Helsinki, Finland
    J Biol Chem 284:25450-8. 2009
    ....
  54. pmc Analyzing the effects of delaying aster separation on furrow formation during cytokinesis in the Caenorhabditis elegans embryo
    Lindsay Lewellyn
    Department of Cellular and Molecular Medicine, Ludwig Institute for Cancer Research, University of California, San Diego, La Jolla, CA 92093, USA
    Mol Biol Cell 21:50-62. 2010
    ....
  55. doi Phosphorylated filamin A regulates actin-linked caveolae dynamics
    Olivia Muriel
    Integrin Signaling Laboratory, Department of Vascular Biology, Centro Nacional de Investigaciones Cardiovasculares CNIC, Melchor Fernandez Almagro 3, 28029 Madrid, Spain
    J Cell Sci 124:2763-76. 2011
    ..Inwards trafficking of caveolin-1 requires both the ability of FLNa to bind actin and cycling PKCα-dependent phosphorylation of FLNa on Ser2152 after cell detachment...
  56. pmc Molecular basis of filamin A-FilGAP interaction and its impairment in congenital disorders associated with filamin A mutations
    Fumihiko Nakamura
    Translational Medicine Division, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA, USA
    PLoS ONE 4:e4928. 2009
    ..Mutations in filamin A (FLNa), an essential cytoskeletal protein with multiple binding partners, cause developmental anomalies in humans...
  57. ncbi Actin-binding protein-280 binds the stress-activated protein kinase (SAPK) activator SEK-1 and is required for tumor necrosis factor-alpha activation of SAPK in melanoma cells
    A Marti
    Diabetes Unit and Medical Services, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 021291, USA
    J Biol Chem 272:2620-8. 1997
    ..ABP-280 participates in TNF-alpha signal transduction to SAPKs, in part through the binding of SEK-1...
  58. doi The myriad roles of Anillin during cytokinesis
    Alisa J Piekny
    Department of Biology, Concordia University, Montreal, QC, Canada
    Semin Cell Dev Biol 21:881-91. 2010
    ..Here we review Anillin's interacting partners and the relevance of these interactions in vivo. We also discuss questions of how these interactions are coordinated, and finally provide some perspective regarding Anillin's role in cancer...
  59. doi Bilateral periventricular nodular heterotopia in France: frequency of mutations in FLNA, phenotypic heterogeneity and spectrum of mutations
    G Sole
    Laboratoire de Génétique Humaine, Universite Victor Segalen Bordeaux 2, 33076 Bordeaux Cedex, France
    J Neurol Neurosurg Psychiatry 80:1394-8. 2009
    ..Three additional patients with BPNH-EDS and a mutation in FLNA are described. No phenotype-genotype correlations could be established, but these clinical data sustain the importance of cardiovascular monitoring in FLNA-BPNH patients...
  60. ncbi Profilin promotes barbed-end actin filament assembly without lowering the critical concentration
    F Kang
    Department of Medicine, University of Florida College of Medicine, Gainesville, Florida 32610 0245, USA
    J Biol Chem 274:36963-72. 1999
    ..Our findings indicate that profilin serves as a polymerization catalyst that captures actin monomers from Thymosin-beta4. Actin and ushers actin as a Profilin. Actin complex onto growing barbed filament ends...
  61. doi Differential involvement of sarcomeric proteins in myofibrillar myopathies: a morphological and immunohistochemical study
    Kristl G Claeys
    Institut de Myologie, Groupe Hospitalier Pitie Salpetriere, Paris, France
    Acta Neuropathol 117:293-307. 2009
    ....
  62. pmc Myofibrillar myopathies
    Duygu Selcen
    Department of Neurology, Division of Child Neurology and Neuromuscular Disease Research Laboratory, Mayo Clinic College of Medicine, Mayo Clinic, Rochester, MN, USA
    Neuromuscul Disord 21:161-71. 2011
    ..However, in the majority of the myofibrillar myopathy patients the disease gene awaits discovery...
  63. doi Spatial control of cytokinesis by Cdr2 kinase and Mid1/anillin nuclear export
    Maria Almonacid
    Institut Curie, Centre de Recherche, F 75248 Paris, France
    Curr Biol 19:961-6. 2009
    ..We conclude that Cdr2- and nuclear export-dependent positioning of Mid1 constitute two overlapping mechanisms that relay cell polarity and nuclear positional information to ensure proper division-plane specification...
  64. ncbi Stepping into the ring: the SIN takes on contractile ring assembly
    Rachel H Roberts-Galbraith
    Howard Hughes Medical Institute, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Genes Dev 22:3082-8. 2008
    ..Furthermore, their careful analysis of contractile ring formation may help bridge two existing models of cytokinetic ring formation...
  65. pmc Filamin-A fragment localizes to the nucleus to regulate androgen receptor and coactivator functions
    C J Loy
    Department of Obstetrics and Gynecology, National University of Singapore, Singapore 119074
    Proc Natl Acad Sci U S A 100:4562-7. 2003
    ..This unanticipated role of FLNa adds to the growing evidence for the involvement of cytoskeletal proteins in transcription regulation...
  66. pmc Filamin is required for ring canal assembly and actin organization during Drosophila oogenesis
    M G Li
    Department of Genetics, Cell and Developmental Biology, University of Minnesota, St Paul, Minnesota 55108, USA
    J Cell Biol 146:1061-74. 1999
    ..The genetic and molecular characterization of Drosophila filamin provides the first genetic model system for the analysis of filamin function and regulation during development...
  67. ncbi Acidocalcisomes are functionally linked to the contractile vacuole of Dictyostelium discoideum
    Norma Marchesini
    Laboratory of Molecular Parasitology, Department of Pathobiology, University of Illinois at Urbana Champaign, Urbana, Illinois 61802, USA
    J Biol Chem 277:8146-53. 2002
    ..Together, these results suggest that the mass-dense granules of D. discoideum are homologous to the acidocalcisomes described in protozoan parasites and are linked to the function of the contractile vacuole...
  68. ncbi CD28 and CTLA-4 coreceptor expression and signal transduction
    Christopher E Rudd
    Department of Pathology, Cell Signalling Section, University of Cambridge, Cambridge, UK
    Immunol Rev 229:12-26. 2009
    ....
  69. ncbi A 90 kDa fragment of filamin A promotes Casodex-induced growth inhibition in Casodex-resistant androgen receptor positive C4-2 prostate cancer cells
    Y Wang
    Department of Urology, School of Medicine, University of California Davis, Sacramento, CA, USA
    Oncogene 26:6061-70. 2007
    ..Our results demonstrate that expression of nuclear FlnA is necessary for androgen dependence in these cells...
  70. ncbi Localized mutations in the gene encoding the cytoskeletal protein filamin A cause diverse malformations in humans
    Stephen P Robertson
    Weatherall Institute of Molecular Medicine, Room 304, The John Radcliffe, Headley Way, Oxford OX3 9DS, UK
    Nat Genet 33:487-91. 2003
    ....
  71. pmc Filamin A modulates kinase activation and intracellular trafficking of epidermal growth factor receptors in human melanoma cells
    Jennifer L Fiori
    Laboratories of Clinical Investigation, National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21224 6825, USA
    Endocrinology 150:2551-60. 2009
    ..These results suggest the requirement of FLNa for efficient EGFR kinase activation and the sorting of endocytosed receptors into the degradation pathway...
  72. pmc Novel anti-filamin-A antibody detects a secreted variant of filamin-A in plasma from patients with breast carcinoma and high-grade astrocytoma
    Ozge Alper
    Alper Biotech, LLC, Rockville, Maryland, USA
    Cancer Sci 100:1748-56. 2009
    ..Additional novel cancer biomarkers have been identified and are being developed alongside Alper-p280 for use in diagnosis of breast carcinoma and high-grade astrocytoma, and for use in the evaluation of therapeutic responses...
  73. pmc Rho-dependent control of anillin behavior during cytokinesis
    Gilles R X Hickson
    Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA
    J Cell Biol 180:285-94. 2008
    ..We propose that such interactions occur dynamically and transiently to promote furrow stability...
  74. ncbi Beta2 integrin phosphorylation on Thr758 acts as a molecular switch to regulate 14-3-3 and filamin binding
    Heikki Takala
    Department of Biological and Environmental Science and Nanoscience Center, University of Jyvaskyla, Jyvaskyla, Finland
    Blood 112:1853-62. 2008
    ..Our results suggest that the phosphorylation of beta2 integrins on Thr758 acts as a molecular switch to inhibit filamin binding and allow 14-3-3 protein binding to the integrin cytoplasmic domain, thereby modulating T-cell adhesion...
  75. pmc Cooperative regulation of extracellular signal-regulated kinase activation and cell shape change by filamin A and beta-arrestins
    Mark G H Scott
    Institut Cochin, Dept of Cell Biology, 27 rue du Faubourg St Jacques, 75014 Paris, France
    Mol Cell Biol 26:3432-45. 2006
    ..Our results indicate that betaarr and FLNA cooperate to regulate ERK activation and actin cytoskeleton reorganization...
  76. ncbi The regulation mechanism for the auto-inhibition of binding of human filamin A to integrin
    Ulla Pentikäinen
    Department of Biological and Environmental Science, University of Jyvaskyla, Jyvaskyla, Finland
    J Mol Biol 393:644-57. 2009
    ..This observed force-induced conformational change can also be one of possible mechanisms involved in the regulation of integrin activation...
  77. pmc ASB2 targets filamins A and B to proteasomal degradation
    Mélina L Heuzé
    Universite de Toulouse, Universite Paul Sabatier, Toulouse, France
    Blood 112:5130-40. 2008
    ..Thus, we suggest that ASB2 may regulate hematopoietic cell differentiation by modulating cell spreading and actin remodeling through targeting of filamins for degradation...
  78. ncbi Anillin is a substrate of anaphase-promoting complex/cyclosome (APC/C) that controls spatial contractility of myosin during late cytokinesis
    Wei Meng Zhao
    Department of Biological Sciences, Stanford University, Stanford, California 94305 5020, USA
    J Biol Chem 280:33516-24. 2005
    ..We concluded that anillin functions to maintain the localization of active myosin, thereby ensuring the spatial control of concerted contraction during cytokinesis...
  79. ncbi Filamin A is required for vimentin-mediated cell adhesion and spreading
    Hugh Kim
    Canadian Institutes of Health Group in Matrix Dynamics, University of Toronto, Toronto, Ontario, M5S 3E2, Canada
    Am J Physiol Cell Physiol 298:C221-36. 2010
    ..These data indicate that filamin A associates with vimentin and to protein kinase C-epsilon, thereby enabling vimentin phosphorylation, which is important for beta1 integrin activation and cell spreading on collagen...
  80. pmc The NPIY motif in the integrin beta1 tail dictates the requirement for talin-1 in outside-in signaling
    Bethsaida Nieves
    Center for Cell Biology and Cancer Research, Albany Medical College, 47 New Scotland Avenue, Albany, New York 12208, USA
    J Cell Sci 123:1216-26. 2010
    ..Our studies emphasize the importance of understanding the mechanisms that regulate the differential binding FLNa and talin-1 to the beta1 tail downstream of integrin activation in promoting integrin function...
  81. doi Filamin a mediates HGF/c-MET signaling in tumor cell migration
    Alex Xianghua Zhou
    Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, University of Gothenburg, Goteborg, Sweden
    Int J Cancer 128:839-46. 2011
    ..These data suggest that FLNA is one of the important regulators of c-MET signaling and HGF-induced tumor cell migration...
  82. pmc Asymmetric distribution of the apical plasma membrane during neurogenic divisions of mammalian neuroepithelial cells
    Yoichi Kosodo
    Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    EMBO J 23:2314-24. 2004
    ..Our results support a novel concept for the cell biological basis of asymmetric, neurogenic divisions of neuroepithelial cells in the mammalian central nervous system...
  83. ncbi Interaction of p38 and Sp1 in a mechanical force-induced, beta 1 integrin-mediated transcriptional circuit that regulates the actin-binding protein filamin-A
    Mario D'Addario
    Canadian Institutes of Health Research Group in Matrix Dynamics, University of Toronto, Ontario, Canada
    J Biol Chem 277:47541-50. 2002
    ....
  84. pmc Evidence for multisite ligand binding and stretching of filamin by integrin and migfilin
    Sujay Subbayya Ithychanda
    Department of Molecular Cardiology, Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, OH 44195, USA
    Biochemistry 50:4229-31. 2011
    ..The data suggest that filamin is mechanically stretched by integrin or migfilin via a multisite binding mechanism for regulating cytoskeleton and integrin-mediated cell adhesion...
  85. ncbi Mechanical unfolding of single filamin A (ABP-280) molecules detected by atomic force microscopy
    S Furuike
    Materials Science, Graduate School of Science and Engineering, Shizuoka University, Japan
    FEBS Lett 498:72-5. 2001
    ..Based on these results, we discuss here the physiological implications of the mechanical properties of single filamin A molecules...
  86. ncbi Clinical and morphological phenotype of the filamin myopathy: a study of 31 German patients
    Rudolf A Kley
    Department of Neurology, Neuromuscular Center Ruhrgebiet, Ruhr University Bochum, Bochum, Germany
    Brain 130:3250-64. 2007
    ..Therapy is so far limited to symptomatic treatment. The German filaminopathy cohort, the largest group of patients studied so far, shares phenotypic features with LGMD and presents with characteristic histopathological findings of MFM...
  87. ncbi G protein-coupled receptor kinase regulates dopamine D3 receptor signaling by modulating the stability of a receptor-filamin-beta-arrestin complex. A case of autoreceptor regulation
    Kyeong Man Kim
    Department of Pharmacology, College of Pharmacy, Chonnam National University, Kwang Ju, Korea
    J Biol Chem 280:12774-80. 2005
    ..These findings provide a novel GRK-based mechanism for regulating D3R signaling potential and provide insight for interpreting D3R autoreceptor behavior...
  88. ncbi ECSM2, an endothelial specific filamin a binding protein that mediates chemotaxis
    Laura Jane Armstrong
    Angiogenesis Laboratory, Cancer Research UK, Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford
    Arterioscler Thromb Vasc Biol 28:1640-6. 2008
    ..We aimed to characterize the expression and function of a novel transcript that bioinformatics analysis predicted to be endothelial specific, called endothelial-specific molecule-2 (ECSM2)...
  89. ncbi When contractile proteins go bad: the sarcomere and skeletal muscle disease
    Nigel G Laing
    Centre for Medical Research, University of Western Australia, West Australian Institute for Medical Research, Nedlands, Western Australia, Australia
    Bioessays 27:809-22. 2005
    ..The affected patients and families hope for help sooner rather than later. The onus is on all scientists researching sarcomeric proteins to help develop treatments...
  90. pmc An active biopolymer network controlled by molecular motors
    Gijsje H Koenderink
    Department of Physics and Harvard School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138
    Proc Natl Acad Sci U S A 106:15192-7. 2009
    ..This design principle may be applicable to engineering novel biologically inspired, active materials that adjust their own stiffness by internal catalytic control...
  91. pmc Profilin I is essential for cell survival and cell division in early mouse development
    W Witke
    European Molecular Biology Laboratory Monterotondo, Mouse Biology Program, Via Ramarini 32, 00016 Monterotondo, Italy
    Proc Natl Acad Sci U S A 98:3832-6. 2001
    ..Our results indicate that mouse profilin I is an essential protein that has dosage-dependent effects on cell division and survival during embryogenesis...
  92. ncbi Identification of candidate prostate cancer biomarkers in prostate needle biopsy specimens using proteomic analysis
    Jian feng Lin
    Key Laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005, China
    Int J Cancer 121:2596-605. 2007
    ..The proteins identified by this approach may be useful molecular targets for PCa diagnostics and therapeutics...
  93. pmc Regulation of contractile vacuole formation and activity in Dictyostelium
    Fei Du
    Section of Cell and Developmental Biology, Division of Biological Sciences, University of California San Diego, La Jolla, CA 92093 0380, USA
    EMBO J 27:2064-76. 2008
    ..Our studies help define the pathways controlling CV function...
  94. pmc Identification and characterization of multiple similar ligand-binding repeats in filamin: implication on filamin-mediated receptor clustering and cross-talk
    Sujay Subbayya Ithychanda
    Department of Molecular Cardiology, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA
    J Biol Chem 284:35113-21. 2009
    ....
  95. ncbi Dictyostelium amoebae that lack G-actin-sequestering profilins show defects in F-actin content, cytokinesis, and development
    M Haugwitz
    Max Planck Institut fur Biochemie, Martinsried, Federal Republic of Germany
    Cell 79:303-14. 1994
    ..The aberrant phenotype could be rescued by reintroducing a functional profilin I or profilin II gene. The data in this study suggest that profilin functions in Dictyostelium amoebae primarily as an actin-sequestering protein...
  96. pmc Phosphorylation facilitates the integrin binding of filamin under force
    Harvey S Chen
    Molecular Cell Biomechanics Laboratory, Department of Bioengineering, University of California, Berkeley, California, USA
    Biophys J 97:3095-104. 2009
    ..We believe the results of this study implicate filamin-A as a tunable mechanosensor, where its sensitivity can be modulated by the degree of phosphorylation...
  97. pmc Stabilization of the actomyosin ring enables spermatocyte cytokinesis in Drosophila
    Philip Goldbach
    Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, ON, M5G 1L7, Canada
    Mol Biol Cell 21:1482-93. 2010
    ..Our results show that the anillin-septin and cadherin-catenin complexes can serve as alternative cassettes to promote tight physical coupling of F-actin and myosin II to the cleavage furrow and successful completion of cytokinesis...
  98. ncbi A new member of the LIM protein family binds to filamin B and localizes at stress fibers
    Toshiro Takafuta
    Department of Medicine, Cardeza Foundation for Hematologic Research, Jefferson Medical College, Philadelphia, Pennsylvania 19041, USA
    J Biol Chem 278:12175-81. 2003
    ..The association between filamin B and FBLP-1 may play a hitherto unknown role in cytoskeletal function, cell adhesion, and cell motility...
  99. ncbi Bilateral periventricular nodular heterotopia due to filamin 1 gene mutation: widespread glomeruloid microvascular anomaly and dysplastic cytoarchitecture in the cerebral cortex
    Akiyoshi Kakita
    Department of Pathology, Brain Research Institute, Niigata University, 1 Asahimachi, Niigata 951 8585, Japan
    Acta Neuropathol 104:649-57. 2002
    ..This case appears to represent an example of BPNH manifesting widespread developmental anomalies within the blood vessels and the cortical cytoarchitecture in the cerebrum...
  100. pmc Anillin binds nonmuscle myosin II and regulates the contractile ring
    Aaron F Straight
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA
    Mol Biol Cell 16:193-201. 2005
    ..We propose a role for anillin in spatially regulating the contractile activity of myosin II during cytokinesis...
  101. ncbi Filamin A is a novel caveolin-1-dependent target in IGF-I-stimulated cancer cell migration
    Dana Ravid
    Department of Biological Regulation, Weizmann Institute of Science, Rehovot 76100, Israel
    Exp Cell Res 314:2762-73. 2008
    ..These data indicate that caveolin-1 specifies filamin A as a novel target for Akt-mediated filamin A Ser-2152 phosphorylation thus mediating the effects of caveolin-1 on IGF-I-induced cancer cell migration...

Research Grants78

  1. Single Cross-Bridge Kinetics in Transgenic Mouse Hearts Expressing FHC Mutations
    Danuta Szczesna Cordary; Fiscal Year: 2010
    ..FHC) is an autosomal dominant disease originating from mutations in genes that encode for the major contractile proteins of the heart, including the ventricular myosin regulatory (RLC) and essential (ELC) light chains...
  2. Single Cross-Bridge Kinetics in Transgenic Mouse Hearts Expressing FHC Mutations
    Julian Borejdo; Fiscal Year: 2009
    ..FHC) is an autosomal dominant disease originating from mutations in genes that encode for the major contractile proteins of the heart, including the ventricular myosin regulatory (RLC) and essential (ELC) light chains...
  3. Pbx Homeodomain Proteins in Skeletal Muscle Differentiation
    LISA A MAVES; Fiscal Year: 2010
    ..skeletal muscle differentiation by sequentially activating groups of genes, including those encoding muscle contractile proteins. The long term goal of this proposal is to elucidate how muscle regulatory factors like Myod activate their ..
  4. Pbx Homeodomain Proteins in Skeletal Muscle Differentiation
    Lisa Maves; Fiscal Year: 2009
    ..skeletal muscle differentiation by sequentially activating groups of genes, including those encoding muscle contractile proteins. The long term goal of this proposal is to elucidate how muscle regulatory factors like Myod activate their ..
  5. Proteasome, protein oxidation, and cardiomyocyte function.
    SAUL POWELL; Fiscal Year: 2009
    ..Earlier studies focused on the role of the Cadependent proteases to explain loss of contractile proteins during myocardial ischemia...
  6. Proteasome, protein oxidation, and cardiomyocyte function.
    Xuejun Wang; Fiscal Year: 2010
    ..Earlier studies focused on the role of the Cadependent proteases to explain loss of contractile proteins during myocardial ischemia...
  7. Myosin Light Chain 2 Phosphorylation in Cardiac Biology
    NATHAN JIDEAMA; Fiscal Year: 2004
    Cardiac muscle contraction is initiated by morphological changes in the contractile proteins of the cardiac myofibrils...
  8. Fetal/adult cerebral/pulmonary artery BK channel adaptat
    DAVID HESSINGER; Fiscal Year: 2005
    ..Developmental changes in VSMCs include changes in contractile proteins, membrane composition, receptors, signal pathways, and ion channels...
  9. FLUORESCENCE STUDIES OF ENZYME STRUCTURE AND FUNCTION
    Sonia Anderson; Fiscal Year: 1992
    ..The first, dealing with the effects of cytoskeletal and contractile proteins on the structure and activity of phosphofructokinase (PFK), was prompted by the association of the enzyme ..
  10. MOLECULAR PATHOGENESIS OF HUMAN DILATED CARDIOMYOPATHY
    SARKIS MARGOSSIAN; Fiscal Year: 1993
    ..These studies with contractile proteins will be extended to evaluate the state of Ca++-regulation during IDC using myofibirls and purified myosin ..
  11. FATIGUE OF SEGMENTAL MOTOR MECHANISMS
    DOUGLAS STUART; Fiscal Year: 1992
    ..of the descending drive provided by suprasegmental centers to a reduction in the activity of muscle contractile proteins. Recently our laboratory has proposed four themes that provide a basis for the systematic evaluation of the ..
  12. EFFECTS OF TRAUMATIC STRESS ON GASTRIC SECRETIONS
    Norman Weisbrodt; Fiscal Year: 1999
    ..intestinal transit, and 4) determine the effects of prolonged reduced smooth muscle contractility on the contractile proteins that effect contraction...
  13. FAILURE OF THE CONTRACTILE PROCESS IN FATIGUE
    Thomas Nosek; Fiscal Year: 1993
    ..What is the immediate cause of fatigue; what is the agent or agents that directly affect the SR and contractile proteins? This question has not as yet been answered...
  14. GENE EXPRESSION DURING DROSOPHILA MYOGENESIS
    Allen Shearn; Fiscal Year: 1999
    ..This work advanced our understanding of contractile proteins, illuminated fundamental aspects of myofibril assembly and function, and provided insights into the ..
  15. REGULATORY ELEMENTS CONTROLLING TROPONIN I EXPRESSION
    STEPHEN KONIECZNY; Fiscal Year: 1990
    ..from the cell cycle, the formation of multinucleated muscle fibers and the coordinate accumulation of the contractile proteins including alpha-actin, myosin heavy chain, myosin light chain, alpha-tropomyosin, troponins C,I and T, and ..
  16. ROLE OF CONTRACTILE PROTEINS IN PLATELET FUNCTION
    Joan Fox; Fiscal Year: 1990
    ..By increasing our knowledge of the function of actin-membrane interactions in other cells, these studies may provide insight into processes such as ligand-induced receptor redistribution and regulation of receptor function...
  17. CYTOSKELETAL PROTEINS IN RETINAL PHOTORECEPTORS
    MICHAEL CHAITIN; Fiscal Year: 1990
    ..This will help us to determine whether the generating outer segments undergo a disorganized breakdown, or if the degeneration follows a methodical sequence of events indicative of a "de-differentiation" of the visual cells...
  18. ETIOLOGY OF CATARACT
    NANCY RAFFERTY; Fiscal Year: 1990
    ..Since similar arrays have been shown in a variety of cultured cells to contain contractile proteins, identification and localization of these proteins in whole lens tissue will be pursued...
  19. CELL NA IN VSM PROTEIN PRODUCTION
    JULIUS ALLEN; Fiscal Year: 1990
    The basic underlying hypothesis of this proposal is that (Na+)i plays a role in regulating the amount of contractile proteins and the number of Na+ pump sites in cultured vascular smooth muscle cells...
  20. PROTEIN PHOSPHATASES IN ENDOTHELIAL CELL PERMEABILITY
    JOE GARCIA; Fiscal Year: 2001
    ..b>Contractile proteins, actin and myosin, control permeability and bioactive agonists such as thrombin induce endothelial cell ..
  21. PROTEIN PHOSPHATASES IN ENDOTHELIAL CELL PERMEABILITY
    JOE GARCIA; Fiscal Year: 2000
    ..b>Contractile proteins, actin and myosin, control permeability and bioactive agonists such as thrombin induce endothelial cell ..
  22. CONTROLLED AND UNCONTROLLABLE CALCIUM RELEASE IN HEART
    Sandor Gyorke; Fiscal Year: 2005
    ..coupling relies on transient release of Ca from the sarcoplasmic reticulum (SR) causing activation of the contractile proteins. While the events leading to initiation of Ca release have been well established and are known to involve ..
  23. PROTEIN PHOSPHATASES IN ENDOTHELIAL CELL PERMEABILITY
    JOE GARCIA; Fiscal Year: 1999
    ..b>Contractile proteins, actin and myosin, control permeability and bioactive agonists such as thrombin induce endothelial cell ..
  24. CONTROLLED AND UNCONTROLLABLE CALCIUM RELEASE IN HEART
    Sandor Gyorke; Fiscal Year: 2009
    ..coupling relies on transient release of Ca from the sarcoplasmic reticulum (SR) causing activation of the contractile proteins. While the events leading to initiation of Ca release have been well established and are known to involve ..
  25. CONTROLLED AND UNCONTROLLABLE CALCIUM RELEASE IN HEART
    Sandor Gyorke; Fiscal Year: 2006
    ..coupling relies on transient release of Ca from the sarcoplasmic reticulum (SR) causing activation of the contractile proteins. While the events leading to initiation of Ca release have been well established and are known to involve ..
  26. CONTROLLED AND UNCONTROLLABLE CALCIUM RELEASE IN HEART
    Sandor Gyorke; Fiscal Year: 2007
    ..coupling relies on transient release of Ca from the sarcoplasmic reticulum (SR) causing activation of the contractile proteins. While the events leading to initiation of Ca release have been well established and are known to involve ..
  27. EXCITATION-THERMOGENIC COUPLING/HEAT PRODUCING MUSCLES
    Barbara Block; Fiscal Year: 1990
    ..The heater organ is a muscle that generates heat but does not produce force; the muscle cells lacks contractile proteins yet have retained the two internal membrane networks found in normal skeletal muscle cells...
  28. Loss of Matrix Regulation and Aortic Aneurysm Development in vivo
    Hiromi Yanagisawa; Fiscal Year: 2011
    ..In addition, ECM has profound effects on smooth muscle cell (SMC) phenotypes and mutations in SMC contractile proteins have been shown to be responsible for subsets of thoracic aortic aneurysms in humans...
  29. MUTATION OF FUNCTIONAL DOMAINS IN CARDIAC TROPONIN C
    JOHN PUTKEY; Fiscal Year: 1990
    ..of the Ca2+ signal and transmission of this information through a cascade of myofibrils regulatory and contractile proteins. The critical role for troponin C in regulation of muscle contraction emphasizes a need to fully understand ..
  30. Cerebrovascular Myosin Light Chain Phosphorylation in Fetus, Newborn, and Adult
    William J Pearce; Fiscal Year: 2011
    ..New confocal colocalization methods are capable of resolving interactions among multiple different contractile proteins in these small arteries...
  31. Structure & function of cardiac contractile proteins
    Jeffrey Robbins; Fiscal Year: 2004
    ..Together with the models above, it will provide a comprehensive picture of the alpha-MHC's effects on the development of hypertrophy, dilation and failure. ..
  32. THE ROLE OF CONTRACTILE PROTEINS IN ERYTHROCYTE AGING
    DIANE LIN; Fiscal Year: 1980
    ..The biochemical approach will be on the characterization of the contractile proteins in the intact cell and in isolated form, including studies on the degree of phosphorylation, state of ..
  33. PERICYTE CONTRACTILITY AND MICROVASCULAR PERMEABILITY
    David Shepro; Fiscal Year: 1992
    ..The focus of this study is to demonstrate the: phenotypic expression of specific contractile proteins in pericytes from different microvascular beds; the mechanisms by which pericytes contract and relax; ..
  34. The Duke Pediatric Cardiology Clinical Center
    Page Anderson; Fiscal Year: 2004
    ..The studies have ranged from the contractile proteins to the in vivo heart of the patient and have examined the treatment of dysrhythmias, catheter-based device ..
  35. The Duke Pediatric Cardiology Clinical Center
    Page Anderson; Fiscal Year: 2005
    ..The studies have ranged from the contractile proteins to the in vivo heart of the patient and have examined the treatment of dysrhythmias, catheter-based device ..
  36. The Duke Pediatric Cardiology Clinical Center
    Page Anderson; Fiscal Year: 2003
    ..The studies have ranged from the contractile proteins to the in vivo heart of the patient and have examined the treatment of dysrhythmias, catheter-based device ..
  37. The Duke-UNC-CH Pediatric Cardiology Clinical Center
    Page Anderson; Fiscal Year: 2002
    ..The studies have ranged from the contractile proteins to the in vivo heart of the patient and have examined the treatment of dysrhythmias, catheter-based device ..
  38. The Duke-UNC-CH Pediatric Cardiology Clinical Center
    Page Anderson; Fiscal Year: 2001
    ..The studies have ranged from the contractile proteins to the in vivo heart of the patient and have examined the treatment of dysrhythmias, catheter-based device ..
  39. STUNNED HEART AND CARDIAC SURGERY--PROTEIN KINASE C
    Pedro del Nido; Fiscal Year: 2000
    ..are altered calcium regulation for activation/contraction coupling, and/or the response (sensitivity) of contractile proteins to calcium...
  40. STUNNED HEART AND CARDIAC SURGERY--PROTEIN KINASE C
    Pedro del Nido; Fiscal Year: 1999
    ..are altered calcium regulation for activation/contraction coupling, and/or the response (sensitivity) of contractile proteins to calcium...
  41. MUSCLES RELATED TO HEART DISEASE
    YALE GOLDMAN; Fiscal Year: 1993
    ..The major theme of the Program is the assembly and mechano-chemistry of contractile proteins. It integrates investigations of contractile proteins from genetic regulation of cell differentiation, ..
  42. THE ROLE OF CONTRACTILE PROTEINS IN PLATELETS
    ELENA PUSZKIN; Fiscal Year: 1990
    ..These studies may clarify the complex interactions that occur between the membrane of platelets with the internal cytoskeleton that are active in supporting platelet function during aggregation and/or thrombus formation...
  43. Hsp27 Association with Contractile Proteins
    KHALIL BITAR; Fiscal Year: 2001
    ..colonic smooth muscle that results in the phosphorylation of HSP27 and the association of HSP27 with other contractile proteins, particularly actin, tropomyosin, myosin and caldesmon, leading to smooth muscle contraction...
  44. MECHANISM OF MUSCLE WASTING IN AGING MAN
    K Sreekumaran Nair; Fiscal Year: 2010
    ..old proteins with newly synthesized proteins whereas resistance exercise increases synthesis of specific contractile proteins with effect on replacing old and modified proteins...
  45. Identifying the Genetic and Molecular Basis of Clubfoot
    ANITA BECK; Fiscal Year: 2009
    Tlie overall goal of this project is to understand liow mutations in genes that encode contractile proteins cause clubfoot in a group of disorders called distal arthrogryposis (DA) syndromes and test whether variants in these genes ..
  46. FUNCTIONAL DOMAINS IN CARDIAC TROPONIN C
    JOHN PUTKEY; Fiscal Year: 1990
    ..of the Ca2+ signal and transmission of this information through a cascade of myofibril regulatory and contractile proteins. The critical role for troponin C in regulation of muscle contraction emphasizes a need to fully understand ..
  47. NITRIC OXIDE REGULATION OF SMOOTH MUSCLE TONE
    Keith Jones; Fiscal Year: 1999
    ..Both inhibition of Ca2+- calmodulin activation of the contractile proteins and inhibition of membrane receptor-linked second messenger systems that regulate myofilament Ca2+ ..
  48. Identifying the Genetic and Molecular Basis of Clubfoot
    Anita E Beck; Fiscal Year: 2010
    Tlie overall goal of this project is to understand liow mutations in genes that encode contractile proteins cause clubfoot in a group of disorders called distal arthrogryposis (DA) syndromes and test whether variants in these genes ..
  49. BIOCHEMISTRY OF CONTRACTILE PROTEINS
    David Hartshorne; Fiscal Year: 2009
    ..If successful, they will establish an understanding at a molecular level of a process essential for function in muscle and non-muscle cells, and will facilitate pharmacological intervention. ..
  50. BIOCHEMISTRY OF CONTRACTILE PROTEINS
    David Hartshorne; Fiscal Year: 2007
    ..If successful, they will establish an understanding at a molecular level of a process essential for function in muscle and non-muscle cells, and will facilitate pharmacological intervention. ..
  51. NITRIC OXIDE REGULATION OF SMOOTH MUSCLE TONE
    Keith Jones; Fiscal Year: 2000
    ..Both inhibition of Ca2+- calmodulin activation of the contractile proteins and inhibition of membrane receptor-linked second messenger systems that regulate myofilament Ca2+ ..
  52. NITRIC OXIDE REGULATION OF SMOOTH MUSCLE TONE
    Keith Jones; Fiscal Year: 2002
    ..Both inhibition of Ca2+- calmodulin activation of the contractile proteins and inhibition of membrane receptor-linked second messenger systems that regulate myofilament Ca2+ ..
  53. BIOCHEMISTRY OF CONTRACTILE PROTEINS
    David Hartshorne; Fiscal Year: 2006
    ..If successful, they will establish an understanding at a molecular level of a process essential for function in muscle and non-muscle cells, and will facilitate pharmacological intervention. ..
  54. NITRIC OXIDE REGULATION OF SMOOTH MUSCLE TONE
    Keith Jones; Fiscal Year: 2001
    ..Both inhibition of Ca2+- calmodulin activation of the contractile proteins and inhibition of membrane receptor-linked second messenger systems that regulate myofilament Ca2+ ..
  55. REGULATION OF CONTRACTILE PROTEINS IN HUMAN MYOCARDIUM
    Ronglih Liao; Fiscal Year: 1999
    ....
  56. REGULATION OF CONTRACTILE PROTEINS IN HUMAN MYOCARDIUM
    Ronglih Liao; Fiscal Year: 2000
    ....
  57. REGULATORY ELEMENTS CONTROLLING TROPONIN I EXPRESSION
    STEPHEN KONIECZNY; Fiscal Year: 1993
    ..from the cell cycle, the formation of multinucleated muscle fibers and the coordinate accumulation of the contractile proteins including alpha-actin, myosin heavy chain, myosin light chain, alpha-tropomyosin, troponins C,I and T, and ..
  58. FAMILIAL HYPERTROPHIC CARDIOMYOPATHY--SARCOMERIC DISEASE
    David Warshaw; Fiscal Year: 2002
    ..disease with a high incidence of premature death due to cardiac failure, has its genetic loci in the contractile proteins in the heart. Thus, FHC may be a disease of the sarcomere, muscle's most basic contractile unit...
  59. Troponin T mutations and Sudden Cardiac Death
    Bjorn Knollmann; Fiscal Year: 2005
    ..Cardiomyopathy (FHC) is an autosomal-dominant disease resulting from mutations in genes encoding cardiac contractile proteins, and an important cause of Sudden Cardiac Death (SCD)...
  60. FAMILIAL HYPERTROPHIC CARDIOMYOPATHY--SARCOMERIC DISEASE
    David Warshaw; Fiscal Year: 2003
    ..disease with a high incidence of premature death due to cardiac failure, has its genetic loci in the contractile proteins in the heart. Thus, FHC may be a disease of the sarcomere, muscle's most basic contractile unit...
  61. GORDON RESEARCH CONFERENCE--MUSCLE CONTRACTILE PROTEINS
    PETER VIBERT; Fiscal Year: 1993
    The proposed conference on Contractile Proteins will be the 19th in a series of Gordon Research Conferences that originated with a conference on cardiac muscle in 1966...
  62. FAMILIAL HYPERTROPHIC CARDIOMYOPATHY--SARCOMERIC DISEASE
    David Warshaw; Fiscal Year: 2000
    ..disease with a high incidence of premature death due to cardiac failure, has its genetic loci in the contractile proteins in the heart. Thus, FHC may be a disease of the sarcomere, muscle's most basic contractile unit...