u4 u6 small nuclear ribonucleoprotein

Summary

Summary: A nuclear RNA-protein complex that plays a role in RNA processing. In the nucleoplasm, the U4-U6 snRNP along with the U5 snRNP preassemble into a single 25S particle that binds to the U1 and U2 snRNPs and the substrate to form mature SPLICEOSOMES. There is also evidence for the existence of individual U4 or U6 snRNPs in addition to their organization as a U4-U6 snRNP.

Top Publications

  1. doi Phosphorylation of human PRP28 by SRPK2 is required for integration of the U4/U6-U5 tri-snRNP into the spliceosome
    Rebecca Mathew
    Department of Cellular Biochemistry MPI for Biophysical Chemistry, Am Fassberg 11, D 37077 Gottingen, Germany
    Nat Struct Mol Biol 15:435-43. 2008
  2. pmc Xenopus LSm proteins bind U8 snoRNA via an internal evolutionarily conserved octamer sequence
    Nenad Tomasevic
    Genetics and Biochemistry Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 1766, USA
    Mol Cell Biol 22:4101-12. 2002
  3. pmc Structure and function of an RNase H domain at the heart of the spliceosome
    Vladimir Pena
    Abteilung Zelluläre Biochemie, Max Planck Institut fur biophysikalische Chemie, Gottingen, Germany
    EMBO J 27:2929-40. 2008
  4. pmc ATP-dependent unwinding of U4/U6 snRNAs by the Brr2 helicase requires the C terminus of Prp8
    Corina Maeder
    Department of Biochemistry and Biophysics, University of California, San Francisco, 600 16th Street, California 94143, USA
    Nat Struct Mol Biol 16:42-8. 2009
  5. pmc Ongoing U snRNP biogenesis is required for the integrity of Cajal bodies
    Ira Lemm
    Department of Cellular Biochemistry, Max Planck Institute for Biophysical Chemistry, D 37077 Gottingen, Germany
    Mol Biol Cell 17:3221-31. 2006
  6. pmc The yeast Prp3 protein is a U4/U6 snRNP protein necessary for integrity of the U4/U6 snRNP and the U4/U6.U5 tri-snRNP
    J G Anthony
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA
    RNA 3:1143-52. 1997
  7. pmc Ubiquitin binding by a variant Jab1/MPN domain in the essential pre-mRNA splicing factor Prp8p
    Priya Bellare
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, IL 60208, USA
    RNA 12:292-302. 2006
  8. pmc Suppressors of a cold-sensitive mutation in yeast U4 RNA define five domains in the splicing factor Prp8 that influence spliceosome activation
    A N Kuhn
    Department of Biomolecular Chemistry, University of Wisconsin Medical School, Madison, Wisconsin 53706 1532, USA
    Genetics 155:1667-82. 2000
  9. ncbi A spliceosomal recycling factor that reanneals U4 and U6 small nuclear ribonucleoprotein particles
    P L Raghunathan
    University of California, San Francisco, School of Medicine, Department of Biochemistry and Biophysics, San Francisco, CA 94143 0448, USA
    Science 279:857-60. 1998
  10. pmc Roles of PRP8 protein in the assembly of splicing complexes
    J D Brown
    Institute of Cell and Molecular Biology, University of Edinburgh, UK
    EMBO J 11:3721-9. 1992

Research Grants

  1. ASSEMBLY AND FUNCTION OF THE U4/U6 SPLICEOSOMAL SNRNP
    DAVID BROW; Fiscal Year: 2000
  2. Interpreting the Structure of the Spliceosome
    MELISSA JURICA; Fiscal Year: 2007
  3. DNA-DIRECTED EFFECTS OF FdUMP(N)
    WILLIAM GMEINER; Fiscal Year: 2007
  4. Interpreting the Structure of the Spliceosome
    MELISSA JURICA; Fiscal Year: 2006
  5. Interpreting the Structure of the Spliceosome
    Melissa S Jurica; Fiscal Year: 2010
  6. Interpreting the Structure of the Spliceosome
    MELISSA JURICA; Fiscal Year: 2006
  7. Mechanisms of 3' Splice Site Selection in S. cerevisiae
    DAVID MCPHEETERS; Fiscal Year: 2005
  8. BIOCHEMISTRY OF PREMRNA SPLICING FACTORS
    David Horowitz; Fiscal Year: 2004
  9. High-throughput screen for inhibitors of human spliceosomes
    Melissa S Jurica; Fiscal Year: 2010

Scientific Experts

Detail Information

Publications141 found, 100 shown here

  1. doi Phosphorylation of human PRP28 by SRPK2 is required for integration of the U4/U6-U5 tri-snRNP into the spliceosome
    Rebecca Mathew
    Department of Cellular Biochemistry MPI for Biophysical Chemistry, Am Fassberg 11, D 37077 Gottingen, Germany
    Nat Struct Mol Biol 15:435-43. 2008
    ..Our results demonstrate a role for SRPK2 in splicing and reveal a previously unknown function for PRP28 in spliceosome assembly...
  2. pmc Xenopus LSm proteins bind U8 snoRNA via an internal evolutionarily conserved octamer sequence
    Nenad Tomasevic
    Genetics and Biochemistry Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 1766, USA
    Mol Cell Biol 22:4101-12. 2002
    ..This purified complex can bind U6 snRNA in vitro but does not bind U3 or U14 snoRNA in vitro, demonstrating that the LSm complex specifically recognizes U8 RNA...
  3. pmc Structure and function of an RNase H domain at the heart of the spliceosome
    Vladimir Pena
    Abteilung Zelluläre Biochemie, Max Planck Institut fur biophysikalische Chemie, Gottingen, Germany
    EMBO J 27:2929-40. 2008
    ..These data suggest that Prp8 employs an RNase H domain to help assemble and stabilize the spliceosomal catalytic core, coordinate the activities of other splicing factors and possibly participate in chemical catalysis of splicing...
  4. pmc ATP-dependent unwinding of U4/U6 snRNAs by the Brr2 helicase requires the C terminus of Prp8
    Corina Maeder
    Department of Biochemistry and Biophysics, University of California, San Francisco, 600 16th Street, California 94143, USA
    Nat Struct Mol Biol 16:42-8. 2009
    ..Because Brr2 activity must be restricted to prevent premature catalytic activation, our results have important implications for fidelity maintenance in the spliceosome...
  5. pmc Ongoing U snRNP biogenesis is required for the integrity of Cajal bodies
    Ira Lemm
    Department of Cellular Biochemistry, Max Planck Institute for Biophysical Chemistry, D 37077 Gottingen, Germany
    Mol Biol Cell 17:3221-31. 2006
    ..Altogether, our data suggest that CBs have a modular structure with distinct domains for spliceosomal U snRNPs and snoRNPs...
  6. pmc The yeast Prp3 protein is a U4/U6 snRNP protein necessary for integrity of the U4/U6 snRNP and the U4/U6.U5 tri-snRNP
    J G Anthony
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA
    RNA 3:1143-52. 1997
    ..U5 tri-snRNPs. Prp3p is homologous to a human protein that is a component of U4/U6 snRNPs, exemplifying the conservation of splicing factors between yeast and metazoans...
  7. pmc Ubiquitin binding by a variant Jab1/MPN domain in the essential pre-mRNA splicing factor Prp8p
    Priya Bellare
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, IL 60208, USA
    RNA 12:292-302. 2006
    ..Our results define a new UBD and suggest functional links between ubiquitin and the pre-mRNA splicing machinery...
  8. pmc Suppressors of a cold-sensitive mutation in yeast U4 RNA define five domains in the splicing factor Prp8 that influence spliceosome activation
    A N Kuhn
    Department of Biomolecular Chemistry, University of Wisconsin Medical School, Madison, Wisconsin 53706 1532, USA
    Genetics 155:1667-82. 2000
    ....
  9. ncbi A spliceosomal recycling factor that reanneals U4 and U6 small nuclear ribonucleoprotein particles
    P L Raghunathan
    University of California, San Francisco, School of Medicine, Department of Biochemistry and Biophysics, San Francisco, CA 94143 0448, USA
    Science 279:857-60. 1998
    ..Addition of purified Prp24 protein regenerates duplex U4/U6 snRNPs for new rounds of splicing. The reannealing reaction catalyzed by Prp24 proceeds more efficiently with snRNPs than with deproteinized snRNAs...
  10. pmc Roles of PRP8 protein in the assembly of splicing complexes
    J D Brown
    Institute of Cell and Molecular Biology, University of Edinburgh, UK
    EMBO J 11:3721-9. 1992
    ....
  11. pmc Fission yeast Prp4p kinase regulates pre-mRNA splicing by phosphorylating a non-SR-splicing factor
    W Schwelnus
    Institute of Genetics, Technical University of Braunschweig, Germany
    EMBO Rep 2:35-41. 2001
    ..These results are consistent with the notion that Prp4p kinase is involved in the control of the formation of active spliceosomes, targeting non-SR splicing factors...
  12. pmc A doughnut-shaped heteromer of human Sm-like proteins binds to the 3'-end of U6 snRNA, thereby facilitating U4/U6 duplex formation in vitro
    T Achsel
    Institut fur Molekularbiologie und Tumorforschung, Universitat Marburg, Emil Mannkopff Strasse 2, 35037 Marburg, Germany
    EMBO J 18:5789-802. 1999
    ..Finally, we show that the LSm proteins facilitate the formation of U4/U6 RNA duplices in vitro, suggesting that the LSm proteins may play a role in U4/U6 snRNP formation...
  13. pmc Characterization of U6 snRNA-protein interactions
    V P Vidal
    Institute of Cell and Molecular Biology, University of Edinburgh, United Kingdom
    RNA 5:1470-81. 1999
    ..Interestingly, the Lsm proteins associate efficiently with the 3' half of U6, which contains the 3' stem-loop and uridine-rich 3' end, suggesting that the Lsm and Sm proteins may recognize similar features in RNAs...
  14. ncbi RNA unwinding in U4/U6 snRNPs requires ATP hydrolysis and the DEIH-box splicing factor Brr2
    P L Raghunathan
    Department of Biochemistry and Biophysics, University of California, San Francisco 94143 0448, USA
    Curr Biol 8:847-55. 1998
    ..Because these helices must be disrupted to activate the spliceosome for catalysis, this rearrangement is believed to be tightly regulated in vivo...
  15. doi Localization of Prp8, Brr2, Snu114 and U4/U6 proteins in the yeast tri-snRNP by electron microscopy
    Irina Häcker
    Department of Cellular Biochemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, D 37077 Gottingen, Germany
    Nat Struct Mol Biol 15:1206-12. 2008
    ..The head and arm adopt variable relative positions. This molecular organization and dynamics suggest possible scenarios for structural events during catalytic activation...
  16. pmc A role for ubiquitin in the spliceosome assembly pathway
    Priya Bellare
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, 2205 Tech Drive, Evanston, Illinois 60208, USA
    Nat Struct Mol Biol 15:444-51. 2008
    ..Finally, we show that the conserved splicing factor Prp8 is ubiquitinated within purified triple snRNPs. These results reveal a previously unknown ubiquitin-dependent mechanism for controlling the pre-mRNA splicing pathway...
  17. pmc The DEAH-box protein PRP22 is an ATPase that mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes
    J D Wagner
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    EMBO J 17:2926-37. 1998
    ..Significantly, these data suggest that the DEAH-box proteins act directly on RNA substrates within the spliceosome...
  18. pmc Crystal structure of the beta-finger domain of Prp8 reveals analogy to ribosomal proteins
    Kui Yang
    Department of Biochemistry and Molecular Genetics, Anschutz Medical Campus, University of Colorado Denver, Aurora, CO 80045, USA
    Proc Natl Acad Sci U S A 105:13817-22. 2008
    ..These results also demonstrate an analogy between a spliceosomal protein and ribosomal proteins that insert extensions into folded rRNAs and stabilize the ribosome...
  19. pmc The human U5-200kD DEXH-box protein unwinds U4/U6 RNA duplices in vitro
    B Laggerbauer
    Institut fur Molekularbiologie und Tumorforschung, Philipps Universitat Marburg, Emil Mannkopff Strasse 2, D 35037 Marburg, Germany
    Proc Natl Acad Sci U S A 95:4188-92. 1998
    ..The RNA unwinding activity was found to reside exclusively with the U5-200kD DEXH-box protein. Our data raise the interesting possibility that this RNA helicase catalyzes unwinding of the U4/U6 RNA duplex in the spliceosome...
  20. pmc A conserved Lsm-interaction motif in Prp24 required for efficient U4/U6 di-snRNP formation
    Stephen D Rader
    Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California 94143 0448, USA
    RNA 8:1378-92. 2002
    ..We conclude that the conserved C-terminal motif of Prp24 interacts with the Lsm proteins to promote U4/U6 formation...
  21. pmc Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP
    A Gottschalk
    European Molecular Biology Laboratory EMBL, Protein and Peptide Group, Meyerhofstr 1, D 69117 Heidelberg
    EMBO J 18:4535-48. 1999
    ..This suggests that these proteins, at least in part, are also present in a [U2.U4/U6.U5] tetra-snRNP complex...
  22. ncbi Mutations in HPRP3, a third member of pre-mRNA splicing factor genes, implicated in autosomal dominant retinitis pigmentosa
    Christina F Chakarova
    Department of Molecular Genetics, Institute of Ophthalmology, University College London, 11 43 Bath Street, London EC1V 9EV, UK
    Hum Mol Genet 11:87-92. 2002
    ..The identification of mutations in a third pre-mRNA splicing factor gene further highlights a novel mechanism of photoreceptor degeneration due to defects in the splicing process...
  23. doi Structural elucidation of a PRP8 core domain from the heart of the spliceosome
    Dustin B Ritchie
    Department of Biochemistry, 474 Medical Sciences Building, University of Alberta, Edmonton, Alberta T6G2H7, Canada
    Nat Struct Mol Biol 15:1199-205. 2008
    ....
  24. pmc p110, a novel human U6 snRNP protein and U4/U6 snRNP recycling factor
    Mathias Bell
    Institut fur Biochemie, Justus Liebig Universitat Giessen, Heinrich Buff Ring 58, D 35392 Giessen, Germany
    EMBO J 21:2724-35. 2002
    ..In summary, p110 represents the human ortholog of Prp24, and associates only transiently with U6 and U4/U6 snRNPs during the recycling phase of the spliceosome cycle...
  25. ncbi Cloning of human PRP4 reveals interaction with Clk1
    T Kojima
    Department of Functional Genomics, Medical Research Institute, Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo ku, Tokyo 113 8510, Japan
    J Biol Chem 276:32247-56. 2001
    ..These findings suggest that the NH(2)-terminal region of hPRP4 may play regulatory roles under an unidentified signal transduction pathway through Clk1...
  26. ncbi Central region of the human splicing factor Hprp3p interacts with Hprp4p
    Juana Maria Gonzalez-Santos
    Program in Lung Biology Research, Hospital for Sick Children, Toronto, Ontario, Canada
    J Biol Chem 277:23764-72. 2002
    ....
  27. pmc Distinct domains of splicing factor Prp8 mediate different aspects of spliceosome activation
    Andreas N Kuhn
    Department of Biomolecular Chemistry, University of Wisconsin Medical School, Madison, WI 53706 1532, USA
    Proc Natl Acad Sci U S A 99:9145-9. 2002
    ..Furthermore, additional genetic interactions with U4-cs1 support a two-state model for this RNA conformational switch and implicate another splicing factor, Prp31, in Prp8-mediated spliceosome activation...
  28. pmc Assembly of Snu114 into U5 snRNP requires Prp8 and a functional GTPase domain
    Tamara J Brenner
    Department of Biochemistry and Biophysics, University of California, San Francisco 94143 2200, USA
    RNA 12:862-71. 2006
    ..Since Prp8 is thought to regulate the activity of the DEAD-box ATPases, this strategy of snRNP assembly could ensure that Prp8 activity is itself regulated by a GTP-dependent mechanism...
  29. pmc Genetic analysis reveals a role for the C terminus of the Saccharomyces cerevisiae GTPase Snu114 during spliceosome activation
    Tamara J Brenner
    Department of Biochemistry and Biophysics, University of California, San Francisco, California 94143 2200, USA
    Genetics 170:1063-80. 2005
    ..We propose that GTP hydrolysis results in a rearrangement between Prp8 and the C terminus of Snu114 that leads to release of U1 and U4, thus activating the spliceosome for catalysis...
  30. ncbi Structure of a multipartite protein-protein interaction domain in splicing factor prp8 and its link to retinitis pigmentosa
    Vladimir Pena
    AG Röntgenkristallographie, Max Planck Institut fur biophysikalische Chemie, Am Fassberg 11, D 37077 Gottingen, Germany
    Mol Cell 25:615-24. 2007
    ..We conclude that the expanded Prp8 Jab1/MPN domain represents a pseudoenzyme converted into a protein-protein interaction platform and that dysfunction of this platform underlies Retinitis pigmentosa...
  31. ncbi Identification and characterization of human genes encoding Hprp3p and Hprp4p, interacting components of the spliceosome
    A Wang
    Division of Respiratory Research, Hospital for Sick Children, University of Toronto, Toronto, Ontario M5G 1X8, Canada
    Hum Mol Genet 6:2117-26. 1997
    ..Hprp3p and Hprp4p have been shown to interact with each other and the first 100 amino acids of Hprp3p are not essential for this interaction. These experiments suggest that both Hprp3p and Hprp4p are components of human spliceosomes...
  32. pmc prp8 mutations that cause human retinitis pigmentosa lead to a U5 snRNP maturation defect in yeast
    Kum Loong Boon
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, King s Buildings, Mayfield Road, Edinburgh EH9 3JR, UK
    Nat Struct Mol Biol 14:1077-83. 2007
    ..We therefore propose a novel assembly pathway for U5 snRNP complexes that is disrupted by mutations that cause human RP...
  33. pmc Hierarchical, clustered protein interactions with U4/U6 snRNA: a biochemical role for U4/U6 proteins
    Stephanie Nottrott
    Department of Cellular Biochemistry, Max Planck Institute of Biophysical Chemistry, Gottingen, Germany
    EMBO J 21:5527-38. 2002
    ..This uneven clustering of the U4/U6 snRNP-specific proteins on U4/U6 snRNA is consistent with a sequential dissociation of the U4/U6 duplex prior to spliceosome catalysis...
  34. ncbi Association of PAP-1 and Prp3p, the products of causative genes of dominant retinitis pigmentosa, in the tri-snRNP complex
    Hiroshi Maita
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo 060 0812, Japan
    Exp Cell Res 302:61-8. 2005
    ..These results also suggest that splicing factors implicated in adRP contribute alone or mutually to proper splicing in the retina and that loss of their functions leads to onset of adRP...
  35. pmc Mammalian PRP4 kinase copurifies and interacts with components of both the U5 snRNP and the N-CoR deacetylase complexes
    Graham Dellaire
    MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh EH4 2XU, Scotland, UK
    Mol Cell Biol 22:5141-56. 2002
    ....
  36. ncbi Mutations in splicing factor PRPF3, causing retinal degeneration, form detrimental aggregates in photoreceptor cells
    Antonella Comitato
    Department of Biomedical Sciences, University of Modena and Reggio Emilia, Modena, Italy
    Hum Mol Genet 16:1699-707. 2007
    ..Our data suggest that the mutant protein has a cell-specific dominant effect in rod photoreceptors while appears not to be harmful to epithelial and fibroblast cells...
  37. ncbi Multiple genetic and biochemical interactions of Brr2, Prp8, Prp31, Prp1 and Prp4 kinase suggest a function in the control of the activation of spliceosomes in Schizosaccharomyces pombe
    Claudia A Bottner
    Institute of Genetics, Technical University of Braunschweig, Spielmannstrasse 7, 38106 Braunschweig, Germany
    Curr Genet 48:151-61. 2005
    ..These data are consistent with the notion that in fission yeast phosphorylation of Prp1 by Prp4 kinase is involved in the activation of pre-catalytic spliceosomes...
  38. ncbi Isolation of S. cerevisiae snRNPs: comparison of U1 and U4/U6.U5 to their human counterparts
    P Fabrizio
    Institut fur Molekularbiologie und Tumorforschung, Phillipps Universität, Marburg, Germany
    Science 264:261-5. 1994
    ..U5 snRNPs are significantly similar. The preparative isolation of yeast snRNPs will allow the cloning as well as genetic and phylogenetic analysis of snRNP proteins which will accelerate our understanding of their function...
  39. pmc Protein 61K, encoded by a gene (PRPF31) linked to autosomal dominant retinitis pigmentosa, is required for U4/U6*U5 tri-snRNP formation and pre-mRNA splicing
    Olga V Makarova
    Max Planck Institute for Biophysical Chemistry, Department of Cellular Biochemistry, Am Fassberg 11, D 37077 Gottingen, Germany
    EMBO J 21:1148-57. 2002
    ..Thus, our studies suggest that disruptions in tri-snRNP formation and function resulting from mutations in the 61K protein may contribute to the manifestation of this disease...
  40. ncbi Binding of the human Prp31 Nop domain to a composite RNA-protein platform in U4 snRNP
    Sunbin Liu
    Abteilung Zelluläre Biochemie, Max Planck Institut fur biophysikalische Chemie, Am Fassberg 11, D 37077 Gottingen, Germany
    Science 316:115-20. 2007
    ..Yeast two-hybrid analyses suggest a link between retinitis pigmentosa and an aberrant hPrp31-hPrp6 interaction that blocks U4/U6-U5 tri-snRNP formation...
  41. pmc The 65 and 110 kDa SR-related proteins of the U4/U6.U5 tri-snRNP are essential for the assembly of mature spliceosomes
    O V Makarova
    Max Planck Institute for Biophysical Chemistry, Department of Cellular Biochemistry, Am Fassberg 11, D 37077 Gottingen, Germany
    EMBO J 20:2553-63. 2001
    ..Moreover, since both proteins contain an N-terminal RS domain, they could mediate the association of the tri-snRNP with pre-spliceosomes by interaction with members of the SR protein family...
  42. pmc A new cyclophilin and the human homologues of yeast Prp3 and Prp4 form a complex associated with U4/U6 snRNPs
    D S Horowitz
    Cold Spring Harbor Laboratory, New York 11724, USA
    RNA 3:1374-87. 1997
    ..The third protein in the complex is a new cyclophilin. Cyclophilins have been proposed to act as chaperones in a variety of cellular processes, and we discuss some possible roles of this U4/U6 snRNP-associated cyclophilin...
  43. pmc RNAi knockdown of hPrp31 leads to an accumulation of U4/U6 di-snRNPs in Cajal bodies
    Nina Schaffert
    Department of Cellular Biochemistry, Max Planck Institute of Biophysical Chemistry, Gottingen, Germany
    EMBO J 23:3000-9. 2004
    ..In contrast, U5 snRNPs largely remain in nucleoplasmic speckles. Our data support the idea that CBs may play a role in tri-snRNP recycling...
  44. ncbi Suppression of multiple substrate mutations by spliceosomal prp8 alleles suggests functional correlations with ribosomal ambiguity mutants
    Charles C Query
    Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Mol Cell 14:343-54. 2004
    ..This mechanistic commonality suggests that alteration of rearrangements represents an evolutionarily convenient way of modulating substrate selectivity...
  45. pmc The biochemical defects of prp4-1 and prp6-1 yeast splicing mutants reveal that the PRP6 protein is required for the accumulation of the [U4/U6.U5] tri-snRNP
    F Galisson
    , Institut Pasteur, Paris, France
    Nucleic Acids Res 21:1555-62. 1993
    ..These results establish that the PRP6 protein is required for the accumulation of the [U4/U6.U5] tri-snRNP. We found no evidence for the presence of the PRP6 protein in the U4/U6 particle...
  46. pmc The prp1+ gene required for pre-mRNA splicing in Schizosaccharomyces pombe encodes a protein that contains TPR motifs and is similar to Prp6p of budding yeast
    S Urushiyama
    Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan
    Genetics 147:101-15. 1997
    ..These results suggest that Prp1p/Zer1p is either directly or indirectly involved in cell cycle progression and/or poly(A)+ RNA nuclear export, in addition to pre-mRNA splicing...
  47. pmc Splicing-independent recruitment of spliceosomal small nuclear RNPs to nascent RNA polymerase II transcripts
    Snehal Bhikhu Patel
    Department of Biochemistry, College of Medicine, School of Molecular and Cellular Biology, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    J Cell Biol 178:937-49. 2007
    ..Collectively, these data indicate that the recruitment of snRNPs to nascent transcripts and the assembly of the spliceosome are uncoupled events...
  48. ncbi Mutations in the pre-mRNA splicing-factor genes PRPF3, PRPF8, and PRPF31 in Spanish families with autosomal dominant retinitis pigmentosa
    Maria Martínez-Gimeno
    Laboratory of Biology and Molecular Genetics, Laboratory of Service, Consorci Sanitari de Terrassa, Hospital de Terrassa, Terrassa, Spain
    Invest Ophthalmol Vis Sci 44:2171-7. 2003
    ....
  49. ncbi Cell-division-cycle defects associated with fission yeast pre-mRNA splicing mutants
    J Potashkin
    Department of Cellular and Molecular Pharmacology, Finch University of Health Sciences, The Chicago Medical School, North Chicago, IL 60064, USA
    Curr Genet 34:153-63. 1998
    ..These results suggest a connection between pre-mRNA splicing and the control of cell division in fission yeast...
  50. ncbi SPF30 is an essential human splicing factor required for assembly of the U4/U5/U6 tri-small nuclear ribonucleoprotein into the spliceosome
    J Rappsilber
    Protein Interaction Laboratory, University of Southern Denmark, Campusvej 55, DK 5230 Odense M, Denmark
    J Biol Chem 276:31142-50. 2001
    ....
  51. pmc A role for the yeast La protein in U6 snRNP assembly: evidence that the La protein is a molecular chaperone for RNA polymerase III transcripts
    B K Pannone
    Departments of Cell Biology, Molecular Biophysics and Biochemistry and Howard Hughes Medical Institute, Yale University School of Medicine, 295 Congress Avenue, New Haven, CT 06536, USA
    EMBO J 17:7442-53. 1998
    ..These results provide evidence that Lhp1p is a molecular chaperone for polymerase III-transcribed RNAs and implicate Lsm8p as a key component in the very early steps of U6 snRNP assembly...
  52. pmc Targeting of U4/U6 small nuclear RNP assembly factor SART3/p110 to Cajal bodies
    David Stanek
    Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
    J Cell Biol 160:505-16. 2003
    ..We propose that U4 and U6 snRNPs accumulate in CBs for the purpose of assembly into U4/U6 snRNPs by SART3/p110...
  53. pmc The human U5-220kD protein (hPrp8) forms a stable RNA-free complex with several U5-specific proteins, including an RNA unwindase, a homologue of ribosomal elongation factor EF-2, and a novel WD-40 protein
    T Achsel
    Institut fur Molekularbiologie und Tumorforschung, Philipps Universitat Marburg, 35037 Marburg, Germany
    Mol Cell Biol 18:6756-66. 1998
    ..Since the 220kD protein is also known to contact both the pre-mRNA and the U5 snRNA, it is in a position to relay the functional state of the spliceosome to the other proteins in the complex and thus modulate their activity...
  54. pmc Functional analysis of the fission yeast Prp4 protein kinase involved in pre-mRNA splicing and isolation of a putative mammalian homologue
    T Gross
    Institut für Genetik Biozentrum, Technische Universitat Braunschweig, Spielmannstrasse 7, D 38106 Braunschweig, Germany
    Nucleic Acids Res 25:1028-35. 1997
    ..pombe. Prp4 and the recombinant yeast/mouse protein kinase phosphorylate the human SR splicing factor ASF/SF2 in vitro in its RS domain...
  55. pmc The human LSm1-7 proteins colocalize with the mRNA-degrading enzymes Dcp1/2 and Xrnl in distinct cytoplasmic foci
    Dierk Ingelfinger
    Department of Cellular Biochemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    RNA 8:1489-501. 2002
    ..Therefore, the foci contain a partially or fully assembled machinery for the degradation of mRNA...
  56. pmc Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins
    S W Stevens
    California Institute of Technology, Division of Biology, 1200 East California Boulevard 147 75, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 96:7226-31. 1999
    ....
  57. pmc Prp8p dissection reveals domain structure and protein interaction sites
    Kum Loong Boon
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, King s Buildings, Mayfield Road, Edinburgh EH9 3JR, UK
    RNA 12:198-205. 2006
    ..Thus, the U5 snRNP protein Snu114p associates with Prp8p in the region 437-770, whereas fragmenting Prp8p at residue 2173 destabilizes its association with Aar2p...
  58. pmc Biochemical and genetic analyses of the U5, U6, and U4/U6 x U5 small nuclear ribonucleoproteins from Saccharomyces cerevisiae
    S W Stevens
    California Institute of Technology, Division of Biology, Pasadena 91125, USA
    RNA 7:1543-53. 2001
    ..We also show that, unlike the human tri-snRNP, the yeast tri-snRNP dissociated upon addition of ATP or dATP...
  59. ncbi Clinical features of a Japanese family with autosomal dominant retinitis pigmentosa associated with a Thr494Met mutation in the HPRP3 gene
    Yuko Wada
    Department of Ophthalmology, Tohoku University School of Medicine, 1 1, Seiryo machi, Aoba ku, 980 77, Sendai, Japan
    Graefes Arch Clin Exp Ophthalmol 242:956-61. 2004
    ..To determine the clinical features of a Japanese family with autosomal dominant retinitis pigmentosa (ADRP) associated with a Thr494Met mutation in the HPRP3 gene...
  60. pmc The network of protein-protein interactions within the human U4/U6.U5 tri-snRNP
    Sunbin Liu
    Department of Cellular Biochemistry, MPI of Biophysical Chemistry, Gottingen, Germany
    RNA 12:1418-30. 2006
    ..Taken together, data presented here provide a detailed picture of the network of protein interactions within the human tri-snRNP...
  61. pmc Functional contacts with a range of splicing proteins suggest a central role for Brr2p in the dynamic control of the order of events in spliceosomes of Saccharomyces cerevisiae
    R W van Nues
    Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3JR, United Kingdom
    Genetics 157:1451-67. 2001
    ..Overall, these protein interaction studies shed light on how splicing factors regulate the order of events in the large spliceosome complex...
  62. pmc Human U4/U6.U5 and U4atac/U6atac.U5 tri-snRNPs exhibit similar protein compositions
    Claudia Schneider
    Department of Cellular Biochemistry, Max Planck Institute of Biophysical Chemistry, D 37077 Gottingen, Germany
    Mol Cell Biol 22:3219-29. 2002
    ....
  63. ncbi Functional recognition of 5' splice site by U4/U6.U5 tri-snRNP defines a novel ATP-dependent step in early spliceosome assembly
    P A Maroney
    Center for RNA Molecular Biology, Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106, USA
    Mol Cell 6:317-28. 2000
    ..We propose that U1 and U5 snRNPs functionally collaborate to recognize and define the 5' splice site prior to establishment of communication with the 3' splice site...
  64. ncbi The human homologue of the yeast splicing factor prp6p contains multiple TPR elements and is stably associated with the U5 snRNP via protein-protein interactions
    E M Makarov
    Institut fur Molekularbiologie und Tumorforschung, Emil Mannkopff Str 2, Philipps Universitat, 35037, Germany
    J Mol Biol 298:567-75. 2000
    ..Consistent with this idea, we show that in vitro translated U5-102kD protein binds to purified 13S U4/U6 snRNPs, which contain, in addition to the Sm proteins, all known U4/U6-specific proteins...
  65. pmc The human U4/U6 snRNP contains 60 and 90kD proteins that are structurally homologous to the yeast splicing factors Prp4p and Prp3p
    J Lauber
    Institut fur Molekularbiologie und Tumorforschung, Marburg, Germany
    RNA 3:926-41. 1997
    ..Based on their structural similarity with essential splicing factors in yeast, the human U4/U6-60kD and 90kD proteins are likely also to play important roles in the mammalian splicing process...
  66. ncbi Interaction of the human autoantigen p150 with splicing snRNPs
    B J Blencowe
    European Molecular Biology Laboratory, Heidelberg, Germany
    J Cell Sci 105:685-97. 1993
    ..Addition of the purified U4/U6.U5 tri-snRNP restores splicing activity to inactivated HeLa nuclear extracts in which splicing had been inhibited by specific depletion of either the U4/U6 or U5 snRNPs...
  67. pmc The yeast PRP19 protein is not tightly associated with small nuclear RNAs, but appears to associate with the spliceosome after binding of U2 to the pre-mRNA and prior to formation of the functional spliceosome
    W Y Tarn
    Institute of Molecular Biology, Academia Sinica, Nankang, Taiwan, Republic of China
    Mol Cell Biol 13:1883-91. 1993
    ..No non-snRNP protein factor has been demonstrated to participate in either step of the spliceosome assembly pathway that PRP19 might be involved in. Thus, PRP19 represents a novel splicing factor...
  68. pmc Prp31p promotes the association of the U4/U6 x U5 tri-snRNP with prespliceosomes to form spliceosomes in Saccharomyces cerevisiae
    E M Weidenhammer
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA
    Mol Cell Biol 17:3580-8. 1997
    ..These results suggest that Prp31p is involved in recruiting the U4/U6 x U5 tri-snRNP to prespliceosome complexes or in stabilizing these interactions...
  69. pmc Yeast ortholog of the Drosophila crooked neck protein promotes spliceosome assembly through stable U4/U6.U5 snRNP addition
    S Chung
    T H Morgan School of Biological Sciences and The Markey Cancer Center, University of Kentucky, Lexington 40506 0225, USA
    RNA 5:1042-54. 1999
    ..Our results indicate that Clf1p acts as a scaffolding protein in spliceosome assembly and suggest that Clf1p may support the cross-intron bridge during the prespliceosome-to-spliceosome transition...
  70. ncbi Mutation in the splicing factor Hprp3p linked to retinitis pigmentosa impairs interactions within the U4/U6 snRNP complex
    Juana Maria Gonzalez-Santos
    Physiology and Experimental Medicine Research Program, Hospital for Sick Children, Toronto, ON, Canada
    Hum Mol Genet 17:225-39. 2008
    ....
  71. pmc The 20kD protein of human [U4/U6.U5] tri-snRNPs is a novel cyclophilin that forms a complex with the U4/U6-specific 60kD and 90kD proteins
    S Teigelkamp
    Institut fur Molekularbiologie und Tumorforschung, Philipps Universitat Marburg, Germany
    RNA 4:127-41. 1998
    ..We discuss possible roles of SnuCyp-20 in the assembly of [U4/U6.U5] tri-snRNPs and/or in conformational changes occurring during the splicing process...
  72. doi Contribution of the individual subunits of protein kinase CK2 and of hPrp3p to the splicing process
    Janka Dörr
    Medizinische Biochemie und Molekularbiologie, Universitat des Saarlandes, Gebäude 44, 66424, Homburg, Germany
    Mol Cell Biochem 316:187-93. 2008
    ..Furthermore, these data support the notion about individual roles for CK2alpha and CK2alpha' in the splicing process...
  73. ncbi The Prp19p-associated complex in spliceosome activation
    Shih Peng Chan
    Institute of Microbiology and Immunology, National Yang Ming University, Shih Pai, Taiwan, Republic of China
    Science 302:279-82. 2003
    ....
  74. pmc The [U4/U6.U5] tri-snRNP-specific 27K protein is a novel SR protein that can be phosphorylated by the snRNP-associated protein kinase
    S Fetzer
    Institut fur Molekularbiologie und Tumorforschung, Marburg, Germany
    RNA 3:344-55. 1997
    ..Thus, the tri-snRNP-specific 27K protein could potentially be involved in SR protein-mediated protein/protein interactions and, additionally, its phosphorylation state could modulate pre-mRNA splicing...
  75. ncbi Two regions promote U11 small nuclear ribonucleoprotein particle binding to a retroviral splicing inhibitor element (negative regulator of splicing)
    Lisa M McNally
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Biol Chem 279:38201-8. 2004
    ..These results indicate that efficient U11 binding to the isolated NRS involves at least two elements in addition to the U11 consensus sequence and may have implications for U11 binding to authentic splicing substrates...
  76. doi Splicing factor SPF30 bridges an interaction between the prespliceosome protein U2AF35 and tri-small nuclear ribonucleoprotein protein hPrp3
    John T Little
    Department of Molecular, Cell, and Developmental Biology and Center for Molecular Biology of RNA, University of California, Santa Cruz, CA 95064, USA
    J Biol Chem 283:8145-52. 2008
    ..Finally, we note that SPF30 and its partner-interacting domains are not conserved in yeast, suggesting this interaction network may play an important role in the complex splicing observed in higher eukaryotes...
  77. ncbi Protein kinase CK2 interacts with the splicing factor hPrp3p
    S Lehnert
    Universitat des Saarlandes, Medizinische Biochemie und Molekularbiologie, Homburg, Germany
    Oncogene 27:2390-400. 2008
    ..In vitro and in vivo splicing assays showed that the splicing activity is significantly influenced by the CK2-hPrp3p interaction. Thus, these data showed that CK2 is involved in the regulation of RNA processing...
  78. pmc The basic domain of Rev from human immunodeficiency virus type 1 specifically blocks the entry of U4/U6.U5 small nuclear ribonucleoprotein in spliceosome assembly
    J Kjems
    Department of Molecular Biology, University of Aarhus, Denmark
    J Virol 67:4769-76. 1993
    ....
  79. pmc A novel set of spliceosome-associated proteins and the essential splicing factor PSF bind stably to pre-mRNA prior to catalytic step II of the splicing reaction
    O Gozani
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115
    EMBO J 13:3356-67. 1994
    ..Finally, we show that SAPs 60 and 90, which are present in both the B and C complexes, are specifically associated with U4 and U6 snRNPs, and thus may have important roles in the functioning of these snRNPs during the splicing reaction...
  80. pmc Recycling of the U12-type spliceosome requires p110, a component of the U6atac snRNP
    Andrey Damianov
    Institut fur Biochemie, Justus Liebig Universitat Giessen, D 35392 Giessen, Germany
    Mol Cell Biol 24:1700-8. 2004
    ..With a U12-dependent in vitro splicing system, we demonstrate that p110 is required for recycling of the U4atac/U6atac snRNP...
  81. ncbi RNA structure and RNA-protein interactions in purified yeast U6 snRNPs
    Ramazan Karaduman
    Max Planck Institute of Biophysical Chemistry, Department of Cellular Biochemistry, Am Fassberg 11, D 37077 Gottingen, Germany
    J Mol Biol 356:1248-62. 2006
    ..Interestingly, we find that the open structure of the yeast U6 snRNA in native snRNPs can also be adopted by human U6 and U6atac snRNAs...
  82. pmc Functional links between the Prp19-associated complex, U4/U6 biogenesis, and spliceosome recycling
    Chun Hong Chen
    Institute of Molecular Biology, Academia Sinica, Nankang, Taiwan, Republic of China
    RNA 12:765-74. 2006
    ....
  83. pmc Progression through the spliceosome cycle requires Prp38p function for U4/U6 snRNA dissociation
    J Xie
    T H Morgan School of Biological Sciences, University of Kentucky, Lexington, KY 40506 0225, USA
    EMBO J 17:2938-46. 1998
    ..Prp38p is the first tri-snRNP-specific protein shown to be dispensable for assembly, but required for conformational changes which lead to catalytic activation of the spliceosome...
  84. pmc A cyclophilin functions in pre-mRNA splicing
    David S Horowitz
    Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
    EMBO J 21:470-80. 2002
    ..These results provide an example of the function of a cyclophilin in a complex process and provide insight into the mechanisms of action of cyclophilins...
  85. ncbi The divergent U12-type spliceosome is required for pre-mRNA splicing and is essential for development in Drosophila
    Leo R Otake
    Department of Cell Biology, Yale University, Howard Hughes Medical Institute, New Haven, CT 06536, USA
    Mol Cell 9:439-46. 2002
    ..Thus, we demonstrate the requirement for the U12 spliceosome in the development of a metazoan organism...
  86. pmc The yeast U5 snRNP coisolated with the U1 snRNP has an unexpected protein composition and includes the splicing factor Aar2p
    A Gottschalk
    Max Planck Institute of Biophysical Chemistry, Department of Cellular Biochemistry, Gottingen, Germany
    RNA 7:1554-65. 2001
    ..Thus, the basic structural scaffold of the Aar2-U5 snRNP seems to be essentially determined by Prp8p, Snu114p, and the Sm proteins...
  87. pmc Reassembly and protection of small nuclear ribonucleoprotein particles by heat shock proteins in yeast cells
    A P Bracken
    Moyne Institute for Preventive Medicine, Microbiology Department, University of Dublin, Trinity College, Ireland
    RNA 5:1586-96. 1999
    ..In addition, our results show that Hsp70 plays a role in snRNP assembly under normal physiological conditions...
  88. ncbi A common core RNP structure shared between the small nucleoar box C/D RNPs and the spliceosomal U4 snRNP
    N J Watkins
    Max Planck Institut fur biophysikalische Chemie, Abteilung Zelluläre Biochemie, Gottingen, Germany
    Cell 103:457-66. 2000
    ..5 kD in vitro. The similarities in structure and function observed between the U4 snRNP (chaperone for U6) and the box C/D snoRNPs raises the interesting possibility that these particles may have evolved from a common ancestral RNP...
  89. pmc Characterization of U4 and U6 interactions with the 5' splice site using a S. cerevisiae in vitro trans-splicing system
    T L Johnson
    Division of Biology, California Institute of Technology, Pasadena, California 91125, USA
    Genes Dev 15:1957-70. 2001
    ..Furthermore, it defines specific nucleotides in U4 and U6 that interact with the 5' splice site at this early stage, even in the absence of base-pairing with the U1 snRNA...
  90. pmc U snRNP assembly in yeast involves the La protein
    D Xue
    Departments of Cell Biology and Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, 295 Congress Avenue, New Haven, CT 06536, USA
    EMBO J 19:1650-60. 2000
    ..These results suggest that, by stabilizing a 3'-extended form of U4 RNA, Lhp1p facilitates efficient Sm protein binding, thus assisting formation of the U4/U6 snRNP...
  91. pmc PSF and p54nrb bind a conserved stem in U5 snRNA
    Rui Peng
    Department of Biological Sciences, Vanderbilt University, Nashville, Tennessee 37235, USA
    RNA 8:1334-47. 2002
    ..Sedimentation analyses show that both proteins associate with spliceosomes and with U4/U6.U5 tri-snPNP...
  92. pmc A conditional U5 snRNA mutation affecting pre-mRNA splicing and nuclear pre-mRNA retention identifies SSD1/SRK1 as a general splicing mutant suppressor
    B G Luukkonen
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nucleic Acids Res 27:3455-65. 1999
    ..Thus we have identified SSD1/SRK1 as a general suppressor of splicing mutants...
  93. pmc Sm protein-Sm site RNA interactions within the inner ring of the spliceosomal snRNP core structure
    H Urlaub
    Max Planck Institute of Biophysical Chemistry, Department of Cellular Biochemistry, Am Fassberg 11, D 37077 Gottingen, Germany
    EMBO J 20:187-96. 2001
    ..Our results thus provide the first evidence that, within the core snRNP, multiple Sm protein-Sm site RNA contacts occur on the inner surface of the heptameric Sm protein ring...
  94. pmc Pseudouridine modification in Caenorhabditis elegans spliceosomal snRNAs: unique modifications are found in regions involved in snRNA-snRNA interactions
    Jeffrey R Patton
    Department of Pathology and Microbiology, University of South Carolina School of Medicine, Columbia, SC 29208, USA
    BMC Mol Biol 6:20. 2005
    ..The positions of Psi in spliceosomal small nuclear RNAs (snRNAs) have been determined for a number of species but not for the snRNAs from Caenorhabditis elegans (C. elegans), a popular experimental model system of development...
  95. pmc PRP38 encodes a yeast protein required for pre-mRNA splicing and maintenance of stable U6 small nuclear RNA levels
    S Blanton
    T H Morgan School of Biological Sciences, University of Kentucky, Lexington 40506 0225
    Mol Cell Biol 12:3939-47. 1992
    ..Curiously, U4 snRNA, normally extensively base paired with U6 snRNA, persists in the virtual absence of U6 snRNA...
  96. ncbi A suppressor of a yeast splicing mutation (prp8-1) encodes a putative ATP-dependent RNA helicase
    D J Jamieson
    Institute of Cell and Molecular Biology, University of Edinburgh, UK
    Nature 349:715-7. 1991
    ..The possible role of this putative helicase in nuclear pre-mRNA splicing is discussed...
  97. pmc PRP4 (RNA4) from Saccharomyces cerevisiae: its gene product is associated with the U4/U6 small nuclear ribonucleoprotein particle
    S P Bjørn
    Hospital for Sick Children, Toronto, Ontario, Canada
    Mol Cell Biol 9:3698-709. 1989
    ..Immunoprecipitations with the antibodies indicated that the PRP4 protein is associated with the U4/U6 small nuclear ribonucleoprotein particle...
  98. pmc PRP4: a protein of the yeast U4/U6 small nuclear ribonucleoprotein particle
    J Banroques
    Division of Biology, California Institute of Technology, Pasadena 91125
    Mol Cell Biol 9:3710-9. 1989
    ..Furthermore, the U5 snRNP could be immunoprecipitated through snRNP-snRNP interactions in the large U4/U5/U6 complex...
  99. ncbi A nuclear-encoded intein in the fungal pathogen Cryptococcus neoformans
    M I Butler
    Department of Biochemistry, University of Otago, Dunedin, New Zealand
    Yeast 18:1365-70. 2001
    ..The Cne PRP8 intein may be a useful drug target in addressing the cryptococcal infections so prevalent in AIDS patients...
  100. doi Structure and function of the GINS complex, a key component of the eukaryotic replisome
    Stuart A MacNeill
    Biomedical Sciences Research Complex, School of Biology, University of St Andrews, North Haugh, St Andrews, Fife KY16 9ST, UK
    Biochem J 425:489-500. 2010
    ....
  101. pmc A yeast splicing factor is localized in discrete subnuclear domains
    D J Elliott
    Howard Hughes Medical Institute, Waltham, MA
    EMBO J 11:3731-6. 1992
    ..The observations indicate that some splicing components are located in discrete subregions of the yeast nucleus, similar to the situation described for the mammalian nucleus...

Research Grants20

  1. ASSEMBLY AND FUNCTION OF THE U4/U6 SPLICEOSOMAL SNRNP
    DAVID BROW; Fiscal Year: 2000
    ..Furthermore, the U4/U6 RNA interaction serves as a paradigm for the study of RNA dynamics, a new and important field of biochemical investigation. ..
  2. Interpreting the Structure of the Spliceosome
    MELISSA JURICA; Fiscal Year: 2007
    ..These studies will move us closer to defining the mechanisms of splice site identification, spliceosome assembly, and splicing catalysis. ..
  3. DNA-DIRECTED EFFECTS OF FdUMP(N)
    WILLIAM GMEINER; Fiscal Year: 2007
    ..These studies greatly enrich our understanding of the unique cytotoxic mechanism for FdUMP[N] compounds towards PC cells. ..
  4. Interpreting the Structure of the Spliceosome
    MELISSA JURICA; Fiscal Year: 2006
    ..These studies will move us closer to defining the mechanisms of splice site identification, spliceosome assembly, and splicing catalysis. ..
  5. Interpreting the Structure of the Spliceosome
    Melissa S Jurica; Fiscal Year: 2010
    ..These studies will move us closer to defining the mechanisms of splice site identification, spliceosome assembly, and splicing catalysis. ..
  6. Interpreting the Structure of the Spliceosome
    MELISSA JURICA; Fiscal Year: 2006
    ..These studies will move us closer to defining the mechanisms of splice site identification, spliceosome assembly, and splicing catalysis. ..
  7. Mechanisms of 3' Splice Site Selection in S. cerevisiae
    DAVID MCPHEETERS; Fiscal Year: 2005
    ..Because these enzymes have important roles in all aspects of cellular RNA metabolism, the results of these studies will be relevant to the study of their functions in other biological contexts. ..
  8. BIOCHEMISTRY OF PREMRNA SPLICING FACTORS
    David Horowitz; Fiscal Year: 2004
    ..Following these interactions through the second step of the reaction will further the understanding of the mechanism of splicing. ..
  9. High-throughput screen for inhibitors of human spliceosomes
    Melissa S Jurica; Fiscal Year: 2010
    ..Identifying chemicals that block the function of this cellular machinery will provide insights into the workings of spliceosomes and potentially serve as leads for drugs to treat disease. ..