u1 small nuclear ribonucleoprotein

Summary

Summary: A nuclear RNA-protein complex that plays a role in RNA processing. In the nucleoplasm, the U1 snRNP along with other small nuclear ribonucleoproteins (U2, U4-U6, and U5) assemble into SPLICEOSOMES that remove introns from pre-mRNA by splicing. The U1 snRNA forms base pairs with conserved sequence motifs at the 5'-splice site and recognizes both the 5'- and 3'-splice sites and may have a fundamental role in aligning the two sites for the splicing reaction.

Top Publications

  1. pmc Crystal structure of human spliceosomal U1 snRNP at 5.5 A resolution
    Daniel A Pomeranz Krummel
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Nature 458:475-80. 2009
  2. pmc A bidirectional SF2/ASF- and SRp40-dependent splicing enhancer regulates human immunodeficiency virus type 1 rev, env, vpu, and nef gene expression
    Massimo Caputi
    Department of Biology, The Johns Hopkins University, Baltimore, MD 21218, USA
    J Virol 78:6517-26. 2004
  3. pmc Regulation of alternative polyadenylation by U1 snRNPs and SRp20
    H Lou
    Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030, USA
    Mol Cell Biol 18:4977-85. 1998
  4. pmc Heterogeneous nuclear ribonucleoproteins H and F regulate the proteolipid protein/DM20 ratio by recruiting U1 small nuclear ribonucleoprotein through a complex array of G runs
    Erming Wang
    Department of Neurology, University of Kentucky, Lexington, Kentucky 40536, USA
    J Biol Chem 284:11194-204. 2009
  5. ncbi U1A RNA-binding domain at 1.8 A resolution
    Peter B Rupert
    Fred Hutchinson Cancer Research Center, USA
    Acta Crystallogr D Biol Crystallogr 59:1521-4. 2003
  6. ncbi An RNA map predicting Nova-dependent splicing regulation
    Jernej Ule
    Howard Hughes Medical Institute, New York, New York 10021, USA
    Nature 444:580-6. 2006
  7. ncbi Altering the RNA-binding mode of the U1A RBD1 protein
    Scott A Showalter
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110, USA
    J Mol Biol 335:465-80. 2004
  8. ncbi Molecular dynamics simulation studies of induced fit and conformational capture in U1A-RNA binding: do molecular substates code for specificity?
    Felicia Pitici
    Chemistry Department and Molecular Biophysics Program, Wesleyan University, Middletown, CT 06459, USA
    Biopolymers 65:424-35. 2002
  9. pmc A study of collective atomic fluctuations and cooperativity in the U1A-RNA complex based on molecular dynamics simulations
    Bethany L Kormos
    Chemistry Department and Molecular Biophysics Program, Wesleyan University, 237 Church St, Middletown, CT 06459, USA
    J Struct Biol 157:500-13. 2007
  10. ncbi The apoptosis-promoting factor TIA-1 is a regulator of alternative pre-mRNA splicing
    P Förch
    Gene Expression Programme European Molecular Biology Laboratory Meyerhofstrasse 1 D 69117, Heidelberg, Germany
    Mol Cell 6:1089-98. 2000

Research Grants

Detail Information

Publications186 found, 100 shown here

  1. pmc Crystal structure of human spliceosomal U1 snRNP at 5.5 A resolution
    Daniel A Pomeranz Krummel
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Nature 458:475-80. 2009
    Human spliceosomal U1 small nuclear ribonucleoprotein particles (snRNPs), which consist of U1 small nuclear RNA and ten proteins, recognize the 5' splice site within precursor messenger RNAs and initiate the assembly of the spliceosome ..
  2. pmc A bidirectional SF2/ASF- and SRp40-dependent splicing enhancer regulates human immunodeficiency virus type 1 rev, env, vpu, and nef gene expression
    Massimo Caputi
    Department of Biology, The Johns Hopkins University, Baltimore, MD 21218, USA
    J Virol 78:6517-26. 2004
    ..4, even when the 5' splice site and the U1 snRNA have been mutated to obtain a perfect complementary match. The ESE characterized here is highly conserved in most viral subtypes...
  3. pmc Regulation of alternative polyadenylation by U1 snRNPs and SRp20
    H Lou
    Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030, USA
    Mol Cell Biol 18:4977-85. 1998
    ....
  4. pmc Heterogeneous nuclear ribonucleoproteins H and F regulate the proteolipid protein/DM20 ratio by recruiting U1 small nuclear ribonucleoprotein through a complex array of G runs
    Erming Wang
    Department of Neurology, University of Kentucky, Lexington, Kentucky 40536, USA
    J Biol Chem 284:11194-204. 2009
    ..Binding of U1 small nuclear ribonucleoprotein (U1snRNP) to DM20 is greatly impaired by mutation of G1 and M2 and depletion of hnRNPH and F...
  5. ncbi U1A RNA-binding domain at 1.8 A resolution
    Peter B Rupert
    Fred Hutchinson Cancer Research Center, USA
    Acta Crystallogr D Biol Crystallogr 59:1521-4. 2003
    ..Alternatively, the malonate (several molecules of which are bound in the vicinity of the RNA-binding surface) may mimic RNA...
  6. ncbi An RNA map predicting Nova-dependent splicing regulation
    Jernej Ule
    Howard Hughes Medical Institute, New York, New York 10021, USA
    Nature 444:580-6. 2006
    ....
  7. ncbi Altering the RNA-binding mode of the U1A RBD1 protein
    Scott A Showalter
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110, USA
    J Mol Biol 335:465-80. 2004
    ..The molecular basis for this alteration in RNA-binding properties is proposed to result from the inability of the RNA to induce a change in the structure of the free protein to produce a high-affinity complex...
  8. ncbi Molecular dynamics simulation studies of induced fit and conformational capture in U1A-RNA binding: do molecular substates code for specificity?
    Felicia Pitici
    Chemistry Department and Molecular Biophysics Program, Wesleyan University, Middletown, CT 06459, USA
    Biopolymers 65:424-35. 2002
    ..7947-7956) and by Williamson (Nature Structural Biology, 2000, Vol. 7, pp. 834-837) suggest an important role for intrinsic molecular architecture and substates other than the native form in the specificity of protein-RNA interactions...
  9. pmc A study of collective atomic fluctuations and cooperativity in the U1A-RNA complex based on molecular dynamics simulations
    Bethany L Kormos
    Chemistry Department and Molecular Biophysics Program, Wesleyan University, 237 Church St, Middletown, CT 06459, USA
    J Struct Biol 157:500-13. 2007
    ..This method has significant implications as a predictive tool regarding cooperativity in the protein-nucleic acid recognition process...
  10. ncbi The apoptosis-promoting factor TIA-1 is a regulator of alternative pre-mRNA splicing
    P Förch
    Gene Expression Programme European Molecular Biology Laboratory Meyerhofstrasse 1 D 69117, Heidelberg, Germany
    Mol Cell 6:1089-98. 2000
    ....
  11. ncbi Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals
    N Abovich
    Howard Hughes Medical Institute, Department of Biology, Brandeis University, Waltham, Massachusetts 02254, USA
    Cell 89:403-12. 1997
    ..Our results show that SF1 interacts strongly with human U2AF65, and that SF1 is a bona fide E complex component. This implies that aspects of these novel cross-intron protein-protein interactions are conserved between yeast and mammals...
  12. ncbi Specific alterations of U1-C protein or U1 small nuclear RNA can eliminate the requirement of Prp28p, an essential DEAD box splicing factor
    J Y Chen
    Department of Molecular Genetics, Ohio State University, Columbus, OH 43210, USA
    Mol Cell 7:227-32. 2001
    ..to counteract the stabilizing effect of the U1-C protein, thereby promoting the dissociation of the U1 small nuclear ribonucleoprotein particle from the 5' splice site...
  13. pmc A comprehensive biochemical and genetic analysis of the yeast U1 snRNP reveals five novel proteins
    A Gottschalk
    Institut fur Molekularbiologie und Tumorforschung, Philipps Universitat Marburg, Germany
    RNA 4:374-93. 1998
    ..Finally, we show that Nam8p/Mud15p contributes to the stability of U1 snRNP...
  14. ncbi SR proteins function in coupling RNAP II transcription to pre-mRNA splicing
    Rita Das
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Mol Cell 26:867-81. 2007
    ..Thus, these factors readily out-compete inhibitory hnRNP proteins, resulting in efficient spliceosome assembly on nascent RNAP II transcripts...
  15. pmc Identification of the proteins of the yeast U1 small nuclear ribonucleoprotein complex by mass spectrometry
    G Neubauer
    European Molecular Biology Laboratory, Heidelberg, Germany
    Proc Natl Acad Sci U S A 94:385-90. 1997
    Here we report the rapid identification of the proteins of the spliceosomal U1 small nuclear ribonucleoprotein (snRNP) from the yeast Saccharomyces cerevisiae by searching mass spectrometric data in genomic sequence databases...
  16. ncbi Specific interactions between proteins implicated in splice site selection and regulated alternative splicing
    J Y Wu
    Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138
    Cell 75:1061-70. 1993
    ..We report that the splicing factors SC35 and SF2/ASF specifically interact with both the integral U1 small nuclear ribonucleoprotein (snRNP U1-70K) and with the 35 kd subunit of the splicing factor U2AF (U2AF35)...
  17. ncbi Pre-spliceosomal binding of U1 small nuclear ribonucleoprotein (RNP) and heterogenous nuclear RNP E1 is associated with suppression of a growth hormone receptor pseudoexon
    Scott A Akker
    Department of Endocrinology, 5th Floor, King George V Block, St Bartholomew s Hospital, West Smithfield, London EC1A 7BE, United Kingdom
    Mol Endocrinol 21:2529-40. 2007
    ..experiments showed that specific binding of heterogenous nuclear ribonucleoprotein E1 (hnRNP E1) and U1 small nuclear ribonucleoprotein (snRNP) in the pre-spliceosomal complex was associated with silencing of pseudoexon splicing...
  18. pmc The importin-beta binding domain of snurportin1 is responsible for the Ran- and energy-independent nuclear import of spliceosomal U snRNPs in vitro
    Jochen Huber
    Department of Cellular Biochemistry, Max Planck Institute of Biophysical Chemistry, D 37077 Gottingen, Germany
    J Cell Biol 156:467-79. 2002
    ..Our results suggest that the nature of the IBB domain modulates the strength and/or site of interaction of impbeta with nucleoporins of the nuclear pore complex, and thus whether or not Ran is required to dissociate these interactions...
  19. ncbi T cell recognition and therapeutic effect of a phosphorylated synthetic peptide of the 70K snRNP protein administered in MR/lpr mice
    Fanny Monneaux
    Institut de Biologie Moléculaire et Cellulaire CNRS, UPR 9021, Strasbourg, France
    Eur J Immunol 33:287-96. 2003
    ..We further demonstrated that P140 is recognized by antibodies from lupus patients and binds to various HLA DR molecules, offering new hope for manipulating T cell response in humans...
  20. ncbi Interactions of Arabidopsis RS domain containing cyclophilins with SR proteins and U1 and U11 small nuclear ribonucleoprotein-specific proteins suggest their involvement in pre-mRNA Splicing
    Zdravko J Lorkovic
    Max F Perutz Laboratories, University Departments at the Vienna Biocenter, Department of Biochemistry, Medical University of Vienna, Dr Bohrgasse 9 3, A 1030 Vienna, Austria
    J Biol Chem 279:33890-8. 2004
    ..Alternatively, binding of CypRS64 to proteins important for 5' splice site recognition suggests its involvement in the dynamics of spliceosome assembly...
  21. pmc Interaction of the U1 snRNP with nonconserved intronic sequences affects 5' splice site selection
    O Puig
    Gene Expression Program, European Molecular Biology Laboratory, D 69117 Heidelberg, Germany
    Genes Dev 13:569-80. 1999
    ..This supports a model where early 5' splice recognition results from a network of interactions established by the splicing machinery with various regions of the pre-mRNA...
  22. pmc Identification of an snRNP-associated kinase activity that phosphorylates arginine/serine rich domains typical of splicing factors
    A Woppmann
    Institut fur Molekularbiologie und Tumorforschung, Marburg, Germany
    Nucleic Acids Res 21:2815-22. 1993
    ..A potential general role for this enzyme in the phosphorylation of splicing factors and its consequences for pre-mRNA splicing regulation are discussed...
  23. pmc An ATP-dependent, Ran-independent mechanism for nuclear import of the U1A and U2B" spliceosome proteins
    M Hetzer
    European Molecular Biology Laboratory EMBL, D 69117 Heidelberg, Germany
    J Cell Biol 148:293-303. 2000
    ..This activity can be solubilized in the presence of elevated MgCl(2). These data suggest that U1A and U2B" import into the nucleus occurs by a hitherto uncharacterized mechanism...
  24. ncbi Nuclear import of the U1A splicesome protein is mediated by importin alpha /beta and Ran in living mammalian cells
    M Hieda
    Department of Cell Biology and Neuroscience, Graduate School of Medicine, Osaka University, 2-2 Yamada-oka and Institute for Molecular and Cellular Biology, Osaka University, 1-3 Yamada-oka, Suita, Osaka 565-0871, Japan
    J Biol Chem 276:16824-32. 2001
    ....
  25. pmc The plant U1 small nuclear ribonucleoprotein particle 70K protein interacts with two novel serine/arginine-rich proteins
    M Golovkin
    Department of Biology and Program in Cell and Molecular Biology, Colorado State University, Fort Collins, Colorado 80523, USA
    Plant Cell 10:1637-48. 1998
    The U1 small nuclear ribonucleoprotein particle (U1 snRNP) 70K protein (U1-70K), one of the three U1 snRNP-specific proteins, is implicated in basic and alternative splicing of nuclear pre-mRNAs...
  26. pmc Identification of eight proteins that cross-link to pre-mRNA in the yeast commitment complex
    D Zhang
    Department of Biology, Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02454, USA
    Genes Dev 13:581-92. 1999
    ....
  27. ncbi Selective modulation of CD4+ T cells from lupus patients by a promiscuous, protective peptide analog
    Fanny Monneaux
    Centre National de la Recherche Scientifique CNRS, Unité Propre de Recherche 9021, Institut de Biologie Moleculaire et Cellulaire, Strasbourg, France
    J Immunol 175:5839-47. 2005
    ..The analog might act as an activator of regulatory T cells or as a partial agonist of TCR...
  28. ncbi The structure of Prp40 FF1 domain and its interaction with the crn-TPR1 motif of Clf1 gives a new insight into the binding mode of FF domains
    Alexander Gasch
    Structural Biology Program, EMBL Heidelberg, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    J Biol Chem 281:356-64. 2006
    ..Our results also revealed that not all FF domains in Prp40 are functionally equivalent. We proposed that at least two different interaction surfaces exist in FF domains that have evolved to recognize distinct binding motifs...
  29. doi Apoptosis-linked changes in the phosphorylation status and subcellular localization of the spliceosomal autoantigen U1-70K
    J Dieker
    Centre National de la Recherche Scientifique, Institut de Biologie Moleculaire et Cellulaire, Strasbourg, France
    Cell Death Differ 15:793-804. 2008
    ....
  30. pmc Improved identification of enriched peptide RNA cross-links from ribonucleoprotein particles (RNPs) by mass spectrometry
    Eva Kühn-Hölsken
    Bioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Nucleic Acids Res 35:e95. 2007
    ..Applying our approach to a partial complex of U2 snRNP allowed us to identify the contact site between the U2 snRNP-specific protein p14/SF3b14a and the branch-site interacting region (BSiR) of U2 snRNA...
  31. ncbi Intramolecular T cell spreading in unprimed MRL/lpr mice: importance of the U1-70k protein sequence 131-151
    Fanny Monneaux
    Centre National de la Recherche Scientifique, Strasbourg, France
    Arthritis Rheum 50:3232-8. 2004
    ....
  32. doi Can donor splice site recognition occur without the involvement of U1 snRNP?
    Michela Raponi
    Human Genetics Division, University of Southampton, Duthie Building Mailpoint 808, Southampton General Hospital, Tremona Road, Southampton SO16 6YD, UK
    Biochem Soc Trans 36:548-50. 2008
    ..We suggest that, in clinical molecular genetics, it is important to evaluate sequence variants for aberrant splicing even in those cases where the variant is not thought to alter the U1 snRNA interaction...
  33. pmc The SRm160/300 splicing coactivator is required for exon-enhancer function
    A G Eldridge
    Banting and Best Department of Medical Research, University of Toronto, Toronto, ON M5G 1L6, Canada
    Proc Natl Acad Sci U S A 96:6125-30. 1999
    ..The results suggest a model for ESE function in which the SRm160/300 splicing coactivator promotes critical interactions between ESE-bound "activators" and the snRNP machinery of the spliceosome...
  34. pmc U1 snRNP-dependent function of TIAR in the regulation of alternative RNA processing of the human calcitonin/CGRP pre-mRNA
    Hui Zhu
    Department of Genetics, School of Medicine, Case Western Reserve University, Ireland Cancer Center, University Hospitals of Cleveland, Cleveland, Ohio 44106, USA
    Mol Cell Biol 23:5959-71. 2003
    ..Conversely, TIAR binding promotes U6 snRNA binding to its target. The synergistic relationship between TIAR and U6 snRNA strongly suggests a novel role of U6 snRNP in regulated alternative RNA processing...
  35. ncbi Arrangement of RNA and proteins in the spliceosomal U1 small nuclear ribonucleoprotein particle
    H Stark
    Institut fur Molekularbiologie und Tumorforschung, Marburg, Germany
    Nature 409:539-42. 2001
    ....
  36. pmc In vitro reconstitution of mammalian U1 snRNPs active in splicing: the U1-C protein enhances the formation of early (E) spliceosomal complexes
    C L Will
    Institut fur Molekularbiologie und Tumorforschung, Philipps Universitat Marburg, Germany
    Nucleic Acids Res 24:4614-23. 1996
    ..Studies with recombinant U1-C protein mutants indicated that the N-terminal domain of U1-C is necessary and sufficient for the stimulation of E complex formation...
  37. ncbi Design of an adenosine analogue that selectively improves the affinity of a mutant U1A protein for RNA
    Ying Zhao
    Department of Chemistry, Wesleyan University, Middletown, CT 06459, USA
    J Am Chem Soc 125:2480-8. 2003
    ..Experiments with other U1A mutant proteins suggest that the stabilization of the complex between the Phe56Ala U1A protein and stem loop 2 RNA is due to a specific interaction between the Phe56Ala U1A protein and A6-4CPh stem loop 2 RNA...
  38. pmc Assembly of the U1 snRNP involves interactions with the backbone of the terminal stem of U1 snRNA
    Timothy S McConnell
    Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University, New Haven, CT 06536, USA
    RNA 9:193-201. 2003
    ..in two adenosines, A65 and A70, in the loop region previously identified as the binding site for the U1 small nuclear ribonucleoprotein (snRNP)-specific U1A protein...
  39. pmc Autoimmune response to U1 small nuclear ribonucleoprotein (U1 snRNP) associated with cytomegalovirus infection
    M M Newkirk
    Division of Rheumatology, The Montreal General Hospital, McGill University, Montreal, Quebec, Canada
    Arthritis Res 3:253-8. 2001
    The induction of autoantibodies to U1 small nuclear ribonucleoprotein (U1 snRNP) complexes is not well understood...
  40. pmc An intronic splicing enhancer binds U1 snRNPs to enhance splicing and select 5' splice sites
    A J McCullough
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Mol Cell Biol 20:9225-35. 2000
    ..We suggest that the G-rich region near vertebrate 5' splice sites promotes accurate splice site recognition by recruiting the U1 snRNP...
  41. ncbi Secondary necrosis is a source of proteolytically modified forms of specific intracellular autoantigens: implications for systemic autoimmunity
    X Wu
    Loma Linda University School of Medicine, California, USA
    Arthritis Rheum 44:2642-52. 2001
    ....
  42. ncbi The appearance of U1 RNP antibody specificities in sequential autoimmune human antisera follows a characteristic order that implicates the U1-70 kd and B'/B proteins as predominant U1 RNP immunogens
    E L Greidinger
    University of Missouri, Columbia, USA
    Arthritis Rheum 44:368-75. 2001
    ..CONCLUSION: Orderly patterns of emergence of U1 RNP peptide antibodies appear to exist in humans. Two peptides, 70 kd and B'/B, show characteristics of early immunogens in the development of human RNP immunity...
  43. ncbi Co-localisation studies of Arabidopsis SR splicing factors reveal different types of speckles in plant cell nuclei
    Zdravko J Lorkovic
    Max F Perutz Laboratories, Medical University of Vienna, Department of Medical Biochemistry, Vienna, Austria
    Exp Cell Res 314:3175-86. 2008
    ....
  44. pmc Sm protein-Sm site RNA interactions within the inner ring of the spliceosomal snRNP core structure
    H Urlaub
    Max Planck Institute of Biophysical Chemistry, Department of Cellular Biochemistry, Am Fassberg 11, D 37077 Gottingen, Germany
    EMBO J 20:187-96. 2001
    ..Our results thus provide the first evidence that, within the core snRNP, multiple Sm protein-Sm site RNA contacts occur on the inner surface of the heptameric Sm protein ring...
  45. ncbi A multiprotein complex mediates the ATP-dependent assembly of spliceosomal U snRNPs
    G Meister
    Max-Planck Institute of Biochemistry, Am Klopferspitz 18a, D-82152 Martinsried, Germany
    Nat Cell Biol 3:945-9. 2001
    ..These data provide the first direct evidence that a complex containing SMN and Gemin2 mediates the active assembly of spliceosomal U snRNPs...
  46. ncbi Modulation of P-element pre-mRNA splicing by a direct interaction between PSI and U1 snRNP 70K protein
    E Labourier
    Department of Molecular and Cell Biology, University of California, Berkeley, 401 Barker Hall 3204, Berkeley, CA 94720, USA
    Mol Cell 8:363-73. 2001
    ..Here we have identified a direct association of PSI with the spliceosomal U1 small nuclear ribonucleoprotein (snRNP) particle in somatic nuclear extracts...
  47. pmc Yeast U1 snRNP-pre-mRNA complex formation without U1snRNA-pre-mRNA base pairing
    H Du
    Department of Biology, Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02454, USA
    RNA 7:133-42. 2001
    ..Only one of these is base pairing between the 5' splice site and the 5' end of U1 snRNA, without which the U1 snRNP-pre-mRNA complex is less stable and has a somewhat altered conformation...
  48. pmc The Drosophila U2 snRNP protein U2A' has an essential function that is SNF/U2B" independent
    A A Nagengast
    Department of Genetics, Case Western Reserve University, School of Medicine, 10900 Euclid Avenue, Cleveland, OH 44106 4955, USA
    Nucleic Acids Res 29:3841-7. 2001
    ..Thus together these data demonstrate that in Drosophila U2A' has an essential function that is unrelated to its role as the partner protein of SNF/U2B"...
  49. ncbi Spliceosomal UsnRNP biogenesis, structure and function
    C L Will
    Max Planck Institute of Biophysical Chemistry, Department of Cellular Biochemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Curr Opin Cell Biol 13:290-301. 2001
    ....
  50. pmc Sequence-specific interaction of U1 snRNA with the SMN complex
    Jeongsik Yong
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6148, USA
    EMBO J 21:1188-96. 2002
    ..These findings indicate that SMN complex interaction with SL1 is sequence-specific and critical for U1 snRNP biogenesis, further supporting the direct role of the SMN complex in RNP biogenesis...
  51. ncbi A new type of mutation causes a splicing defect in ATM
    Franco Pagani
    Molecular Pathology, International Centre for Genetic Engineering and Biotechnology, Trieste, Italy
    Nat Genet 30:426-9. 2002
    ..a new intron-splicing processing element (ISPE) complementary to U1 snRNA, the RNA component of the U1 small nuclear ribonucleoprotein (snRNP)...
  52. pmc The yeast splicing factor Prp40p contains functional leucine-rich nuclear export signals that are essential for splicing
    Mark W Murphy
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Genetics 166:53-65. 2004
    ....
  53. pmc U1A inhibits cleavage at the immunoglobulin M heavy-chain secretory poly(A) site by binding between the two downstream GU-rich regions
    Catherine Phillips
    Molecular Biology and Biochemistry, Rutgers University, Nelson Labs, Room A322, 604 Allison Rd, Piscataway, NJ 08854, USA
    Mol Cell Biol 24:6162-71. 2004
    ..We demonstrate here that U1A binds two (AUGCN(1-3)C) motifs within the 29-nucleotide sequence and inhibits the binding of cleavage stimulatory factor 64K and cleavage at the secretory poly(A) site...
  54. pmc hnRNP H binding at the 5' splice site correlates with the pathological effect of two intronic mutations in the NF-1 and TSHbeta genes
    Emanuele Buratti
    International Centre for Genetic Engineering and Biotechnology, 34012 Trieste, Italy
    Nucleic Acids Res 32:4224-36. 2004
    ..Depletion analyses shows that this protein restricts the accessibility of U1 small nuclear ribonucleoprotein (U1snRNA) to the donor site...
  55. pmc Autoantibodies specific for apoptotic U1-70K are superior serological markers for mixed connective tissue disease
    Danielle Hof
    Department of Biochemistry, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen, Nijmegen, The Netherlands
    Arthritis Res Ther 7:R302-9. 2005
    ..Patients suffering from mixed connective tissue disease (MCTD) produce autoantibodies directed to U1 small nuclear ribonucleoprotein (snRNP), and antibodies against a 70 kDa protein component, the U1-70K (70K) protein, are the most ..
  56. pmc U1 small nuclear RNP from Trypanosoma brucei: a minimal U1 snRNA with unusual protein components
    Zsofia Palfi
    Institut fur Biochemie, Justus Liebig Universität Giessen Heinrich Buff Ring 58, D 35392 Giessen, Germany
    Nucleic Acids Res 33:2493-503. 2005
    ..These data result in a model of the trypanosome U1 snRNP, which deviates substantially from our classical view of the U1 particle and may reflect the special requirements for splicing of a small set of cis-introns in trypanosomes...
  57. ncbi Apoptotic modifications affect the autoreactivity of the U1 snRNP autoantigen
    D Hof
    Department of Biochemistry, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen, Nijmegen, The Netherlands
    Autoimmun Rev 4:380-8. 2005
    ....
  58. ncbi A murine model of mixed connective tissue disease induced with U1 small nuclear RNP autoantigen
    Eric L Greidinger
    Division of Rheumatology and Immunology, University of Miami, Miami, Florida 33136, USA
    Arthritis Rheum 54:661-9. 2006
    ..To test whether immunizing mice with autoantigens closely linked to mixed connective tissue disease (MCTD) could induce an MCTD-like clinical syndrome distinguishable from systemic lupus erythematosus (SLE)...
  59. pmc Complete MALDI-ToF MS analysis of cross-linked peptide-RNA oligonucleotides derived from nonlabeled UV-irradiated ribonucleoprotein particles
    Eva Kühn-Hölsken
    Bioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    RNA 11:1915-30. 2005
    ..This type of analysis is applicable to any type of RNP complex and may be expected to pave the way for the further analysis of protein-RNA complexes in much lower abundance and/or of cross-links that are obtained in low yield...
  60. ncbi An intronic polypyrimidine-rich element downstream of the donor site modulates cystic fibrosis transmembrane conductance regulator exon 9 alternative splicing
    Elisabetta Zuccato
    International Centre for Genetic Engineering and Biotechnology, Padriciano 99, Trieste 34012, Italy
    J Biol Chem 279:16980-8. 2004
    ..exon 9 inclusion, consistent with the facilitating role of TIA-1 in weak 5'-ss recognition by U1 small nuclear ribonucleoprotein. Interestingly, in the presence of a high number of TG repeats and a low number of T repeats in the ..
  61. ncbi Dephosphorylated SRp38 acts as a splicing repressor in response to heat shock
    Chanseok Shin
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    Nature 427:553-8. 2004
    ..We further show that dephosphorylated SRp38 interacts with a U1 small nuclear ribonucleoprotein particle (snRNP) protein, and that this interaction interferes with 5'-splice-site recognition by ..
  62. ncbi Antinuclear antibodies recognize cellular autoantigens driven by apoptosis
    Roxana Ramírez-Sandoval
    Department of Molecular Biology, Centro de Biología Experimental, Universidad Autonoma de Zacatecas, Chepinque 306, Col Lomas de la Soledad, 98040, Zacatecas, Mexico
    Joint Bone Spine 70:187-94. 2003
    ..Present study addresses the issue whether cellular antigens recognised by antinuclear autoantibodies are driven by apoptosis...
  63. pmc Determinants within an 18-amino-acid U1A autoregulatory domain that uncouple cooperative RNA binding, inhibition of polyadenylation, and homodimerization
    Fei Guan
    Rutgers University, Piscataway, New Jersey 08854, USA
    Mol Cell Biol 23:3163-72. 2003
    ..Our molecular dissection of the consequences of conformational changes within an RNP complex presents a powerful example to those studying more complicated pre-mRNA-regulatory systems...
  64. ncbi A functional role for correlated motion in the N-terminal RNA-binding domain of human U1A protein
    Scott A Showalter
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 South Euclid, Box 8231, 63110, St Louis, MO, USA
    J Mol Biol 322:533-42. 2002
    ..Interpretation of the novel RED results and corresponding NMR relaxation data suggests that the loss of collective motions in the mutants could account for their weak RNA-binding...
  65. pmc A general role for splicing enhancers in exon definition
    Bianca J Lam
    Department of Microbiology and Molecular Genetics, College of Medicine, University of California, Irvine 92697 4025, USA
    RNA 8:1233-41. 2002
    ..Our results suggest that ESEs recruit a multicomponent complex that minimally contains components of the splicing machinery required for 5' and 3' splice site selection...
  66. ncbi The fate of the U1 snRNP autoantigen during apoptosis: implications for systemic autoimmunity
    Kelen C R Malmegrim
    Department of Biochemistry, Nijmegen Center of Molecular Life Sciences, University of Nijmegen, Nijmegen, The Netherlands
    Isr Med Assoc J 4:706-12. 2002
    ....
  67. pmc Substitution of an essential adenine in the U1A-RNA complex with a non-polar isostere
    Jacob B Tuite
    Department of Chemistry, Wesleyan University, Middletown, CT 06459, USA
    Nucleic Acids Res 30:5269-75. 2002
    ..The data imply that hydrophobic interactions can compensate energetically for the disruption of the complex hydrogen-bonding network between nucleotide and protein...
  68. ncbi Sex-lethal splicing autoregulation in vivo: interactions between SEX-LETHAL, the U1 snRNP and U2AF underlie male exon skipping
    Alexis A Nagengast
    Department of Genetics, Case Western Reserve University, Cleveland, OH 44106 4955, USA
    Development 130:463-71. 2003
    ....
  69. pmc Proteomic analysis of the U1 snRNP of Schizosaccharomyces pombe reveals three essential organism-specific proteins
    Alain N S Newo
    Institute of Genetics, Technical University of Braunschweig, Spielmannstr 7, 38106 Braunschweig, Germany
    Nucleic Acids Res 35:1391-401. 2007
    ..pombe are essential for growth. We will discuss the differences between the U1 snRNPs with respect to the organism-specific proteins found in the two yeasts and the resulting effect it has on pre-mRNA splicing...
  70. pmc The U1 snRNP base pairs with the 5' splice site within a penta-snRNP complex
    Hadar Malca
    Department of Human Genetics and Molecular Medicine, Sackler School of Medicine, Tel Aviv University, Ramat Aviv 69978, Tel Aviv, Israel
    Mol Cell Biol 23:3442-55. 2003
    ..This fraction is functional in mRNA spliceosome assembly when supplemented with soluble nuclear proteins. The results argue that U1 can bind the 5' splice site in a mammalian preassembled penta-snRNP complex...
  71. pmc Non-snRNP U1A levels decrease during mammalian B-cell differentiation and release the IgM secretory poly(A) site from repression
    Jianglin Ma
    Rutgers University, Department of Molecular Biology and Biochemistry, Nelson Laboratories, Room A322, 604 Allison Road, Piscataway, NJ 08854, USA
    RNA 12:122-32. 2006
    ....
  72. ncbi Apoptotic U1-70 kd is antigenically distinct from the intact form of the U1-70-kd molecule
    Eric L Greidinger
    University of Missouri and Department of Veterans Affairs Medical Research Service, Harry S Truman Memorial Veterans Hospital, Columbia, Missouri, USA
    Arthritis Rheum 46:1264-9. 2002
    ..To determine whether immune responses to an apoptotically modified form of a human lupus autoantigen can be distinguished from immune responses to the intact form of the same antigen...
  73. ncbi Solution structure of the N-terminal RNP domain of U1A protein: the role of C-terminal residues in structure stability and RNA binding
    J M Avis
    MRC Laboratory of Molecular Biology, Cambridge, UK
    J Mol Biol 257:398-411. 1996
    ..The movement of helix C mainly involves changes in the main-chain torsion angles of Thr89, Asp90 and Ser91, the helix thereby acting as a "lid" over the RNA binding surface...
  74. ncbi Crystal structure of the spliceosomal U2B"-U2A' protein complex bound to a fragment of U2 small nuclear RNA
    S R Price
    MRC Laboratory of Molecular Biology, Cambridge, UK
    Nature 394:645-50. 1998
    ....
  75. ncbi A serine/arginine-rich domain in the human U1 70k protein is necessary and sufficient for ASF/SF2 binding
    W Cao
    Department of Pharmacology and Cancer Biology, Levine Science Research Center, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 273:20629-35. 1998
    ..ASF/SF2, SC35, and other members of the serine/arginine family, interact with the 70k protein of the U1 small nuclear ribonucleoprotein. The binding of this protein with ASF/SF2 is thought to enhance recognition of the 5' splice site of ..
  76. ncbi Nuclear accumulation of the U1 snRNP-specific protein C is due to diffusion and retention in the nucleus
    J M Klein Gunnewiek
    Department of Biochemistry, University of Nijmegen, Nijmegen, 6500 HB, The Netherlands
    Exp Cell Res 235:265-73. 1997
    The U1 small nuclear ribonucleoprotein particle (snRNP) has an important function in the early formation of the spliceosome, the multicomponent complex in which pre-mRNA splicing takes place...
  77. pmc Molecular characterization of the spliceosomal proteins U1A and U2B" from higher plants
    G G Simpson
    Cell and Molecular Genetics Department, Scottish Crop Research Institute, Invergowrie, Dundee, UK
    EMBO J 14:4540-50. 1995
    ....
  78. pmc Nucleo-cytoplasmic transport of U snRNPs: definition of a nuclear location signal in the Sm core domain that binds a transport receptor independently of the m3G cap
    U Fischer
    , , Germany
    EMBO J 12:573-83. 1993
    ..We propose that the RNA structure of a given snRNP particle determines at least in part whether the particle's m3G cap is required for nuclear transport or can be dispensed with...
  79. ncbi The Drosophila sex determination gene snf encodes a nuclear protein with sequence and functional similarity to the mammalian U1A snRNP protein
    T W Flickinger
    Department of Genetics, Case Western Reserve University, Cleveland, Ohio 44106 4955
    Genes Dev 8:914-25. 1994
    ..These studies provide the first demonstration, in a multicellular organism, that mutations in a U1 snRNP protein alter splicing in vivo...
  80. ncbi Crystal structure at 1.92 A resolution of the RNA-binding domain of the U1A spliceosomal protein complexed with an RNA hairpin
    C Oubridge
    MRC Laboratory of Molecular Biology, Cambridge, UK
    Nature 372:432-8. 1994
    ..The structure reveals the stereochemical basis for sequence-specific RNA recognition by the RNP domain...
  81. pmc Structure and expression of a plant U1 snRNP 70K gene: alternative splicing of U1 snRNP 70K pre-mRNAs produces two different transcripts
    M Golovkin
    Department of Biology, Colorado State University, Fort Collins 80523, USA
    Plant Cell 8:1421-35. 1996
    The product of the U1 small nuclear ribonucleoprotein particle (U1 snRNP) 70K (U1-70K) gene, a U1 snRNP-specific protein, has been implicated in basic as well as alternative splicing of pre-mRNAs in animals...
  82. pmc The association of the U1-specific 70K and C proteins with U1 snRNPs is mediated in part by common U snRNP proteins
    R L Nelissen
    Department of Biochemistry, University of Nijmegen, The Netherlands
    EMBO J 13:4113-25. 1994
    The U1 small nuclear ribonucleoprotein particle (snRNP)-specific 70K and A proteins are known to bind directly to stem-loops of the U1 snRNA, whereas the U1-C protein does not bind to naked U1 snRNA, but depends on other U1 snRNP protein ..
  83. pmc Commitment of yeast pre-mRNA to the splicing pathway requires a novel U1 small nuclear ribonucleoprotein polypeptide, Prp39p
    S R Lockhart
    T H Morgan School of Biological Sciences, University of Kentucky, Lexington 40506 0225
    Mol Cell Biol 14:3623-33. 1994
    The binding of a U1 small nuclear ribonucleoprotein (snRNP) particle to the 5' splice site region of a pre-mRNA is a primary step of intron recognition...
  84. ncbi Protein-protein interactions and 5'-splice-site recognition in mammalian mRNA precursors
    J D Kohtz
    Department of Biological Sciences, Columbia University, New York, New York 10027
    Nature 368:119-24. 1994
    ..This cooperation is probably achieved by specific interactions between the arginine/serine-rich domain of the splicing factor and a similar region in a U1 snRNP-specific protein...
  85. ncbi Isolation of S. cerevisiae snRNPs: comparison of U1 and U4/U6.U5 to their human counterparts
    P Fabrizio
    Institut fur Molekularbiologie und Tumorforschung, Phillipps Universität, Marburg, Germany
    Science 264:261-5. 1994
    ..U5 snRNPs are significantly similar. The preparative isolation of yeast snRNPs will allow the cloning as well as genetic and phylogenetic analysis of snRNP proteins which will accelerate our understanding of their function...
  86. ncbi Human monoclonal autoantibody fragments from combinatorial antibody libraries directed to the U1snRNP associated U1C protein; epitope mapping, immunolocalization and V-gene usage
    R M Hoet
    Department of Biochemistry, University of Nijmegen, The Netherlands
    Mol Immunol 35:1045-55. 1998
    ..The two cross-reactive antibodies immunoprecipitate the other Sm containing snRNPs as well. Using confocal immunofluorescence microscopy we could show that the major part of the U1C protein is localized within the coiled body structure...
  87. ncbi The fate of U1 snRNP during anti-Fas induced apoptosis: specific cleavage of the U1 snRNA molecule
    W G Degen
    Department of Biochemistry, University of Nijmegen, NL 6500 HB Nijmegen, The Netherlands
    Cell Death Differ 7:70-9. 2000
    ....
  88. ncbi An SC35-like protein and a novel serine/arginine-rich protein interact with Arabidopsis U1-70K protein
    M Golovkin
    Department of Biology and Program in Cell and Molecular Biology, Colorado State University, Fort Collins, Colorado 80523, USA
    J Biol Chem 274:36428-38. 1999
    The U1 small nuclear ribonucleoprotein 70-kDa protein, a U1 small nuclear ribonucleoprotein-specific protein, has been shown to have multiple roles in nuclear precursor mRNA processing in animals...
  89. ncbi The NMR structure of the 38 kDa U1A protein - PIE RNA complex reveals the basis of cooperativity in regulation of polyadenylation by human U1A protein
    L Varani
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Nat Struct Biol 7:329-35. 2000
    ..This mechanism ensures that the activity of PAP enzyme, which is essential to the cell, is only down regulated when U1A is bound to the U1A mRNA...
  90. pmc Yeast ortholog of the Drosophila crooked neck protein promotes spliceosome assembly through stable U4/U6.U5 snRNP addition
    S Chung
    T H Morgan School of Biological Sciences and The Markey Cancer Center, University of Kentucky, Lexington 40506 0225, USA
    RNA 5:1042-54. 1999
    ..Our results indicate that Clf1p acts as a scaffolding protein in spliceosome assembly and suggest that Clf1p may support the cross-intron bridge during the prespliceosome-to-spliceosome transition...
  91. pmc Assembly and localization of the U1-specific snRNP C protein in the amphibian oocyte
    M F Jantsch
    Department of Embryology, Carnegie Institution, Baltimore, Maryland 21210
    J Cell Biol 119:1037-46. 1992
    ..In transfected tissue culture cells, the myc-tagged C protein localized within the nucleus in a speckled pattern similar to that of endogenous U1 snRNPs...
  92. ncbi Structural basis for RanGTP independent entry of spliceosomal U snRNPs into the nucleus
    Daniel Wohlwend
    Abteilung für Molekulare Strukturbiologie, Institut für Mikrobiologie und Genetik and GZMB, Georg August Universitat Gottingen, Justus von Liebig Weg 11, D 37077 Gottingen, Germany
    J Mol Biol 374:1129-38. 2007
    ....
  93. pmc p54nrb is a component of the snRNP-free U1A (SF-A) complex that promotes pre-mRNA cleavage during polyadenylation
    Songchun Liang
    Department of Biochemistry and Molecular Biology, UMDNJ New Jersey Medical School MSB E671, 185 S Orange Avenue, Newark, NJ 07103, USA
    RNA 12:111-21. 2006
    ..We have explored the function of this complex in RNA processing, specifically cleavage and polyadenylation, by performing immunodepletions followed by reconstitution experiments, and have found that p54(nrb) is critical...
  94. pmc The role of positively charged amino acids and electrostatic interactions in the complex of U1A protein and U1 hairpin II RNA
    Michael J Law
    Department of Biochemistry and Molecular Biology, University of Southern California, Los Angeles, CA 90089 9176, USA
    Nucleic Acids Res 34:275-85. 2006
    ..Thus, well-positioned positively charged residues can be important for both initial complex formation and complex maintenance, illustrating the multiple roles of electrostatic interactions in protein-RNA complexes...
  95. doi Hu antigen R (HuR) functions as an alternative pre-mRNA splicing regulator of Fas apoptosis-promoting receptor on exon definition
    José M Izquierdo
    Departamento de Biología Molecular and the Centro de Biología Molecular Severo Ochoa, C S I C, Universidad Autonoma de Madrid, Cantoblanco 28049, Madrid, Spain
    J Biol Chem 283:19077-84. 2008
    ..Taken together, these results support a functional link between HuR as repressor of alternative Fas splicing and the molecular mechanisms modulating programmed cell death...
  96. ncbi A microbead-based system for identifying and characterizing RNA-protein interactions by flow cytometry
    Alexander S Brodsky
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School and the Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Mol Cell Proteomics 1:922-9. 2002
    ..We propose that this strategy, in combination with emerging coded bead systems, can identify RNAs and RNA sequences important for interacting with RNA-binding proteins on genomic scales...
  97. ncbi Identification of nuclear spliceosomal antigens targeted by NOD mouse antibodies following sodium iodide intake
    C Thompson
    University of Cambridge, Department of Pathology, Tennis Court Road, Cambridge, CB2 1QP, UK
    Autoimmunity 39:99-106. 2006
    ..The autoantibody isotypes most consistently represented were IgG2a and IgG2b...
  98. pmc NMR studies of U1 snRNA recognition by the N-terminal RNP domain of the human U1A protein
    P W Howe
    MRC Laboratory of Molecular Biology, Cambridge, UK
    EMBO J 13:3873-81. 1994
    ..The quality of the spectra for this complex of 22 kDa demonstrates the feasibility of NMR investigation of RNA-protein complexes...
  99. pmc The domain of the Bacillus subtilis DEAD-box helicase YxiN that is responsible for specific binding of 23S rRNA has an RNA recognition motif fold
    Shuying Wang
    Department of Structural Biology, Stanford University School of Medicine, California 94305, USA
    RNA 12:959-67. 2006
    ....
  100. ncbi NSSRs/TASRs/SRp38s function as splicing modulators via binding to pre-mRNAs
    Kazuo Fushimi
    Center for Nano Materials and Technology, Japan Advanced Institute of Science and Technology, Nomi City, Ishikawa 923 1292, Japan
    Genes Cells 10:531-41. 2005
    ..5, all containing undifferentiated neural stem cells. Taken together, our results showed that NSSRs modulate alternative splicing via binding to premRNAs during neural differentiation...
  101. pmc Kinetic analysis of the role of the tyrosine 13, phenylalanine 56 and glutamine 54 network in the U1A/U1 hairpin II interaction
    Michael J Law
    Department of Biochemistry and Molecular Biology, University of Southern California Los Angeles, CA 90089 9176, USA
    Nucleic Acids Res 33:2917-28. 2005
    The A protein of the U1 small nuclear ribonucleoprotein particle, interacting with its stem-loop RNA target (U1hpII), is frequently used as a paradigm for RNA binding by recognition motif domains (RRMs)...

Research Grants51

  1. NUCLEAR ACIDIC PROTEIN ANTIGENS IN RHEUMATIC DISEASES
    GORDON SHARP; Fiscal Year: 1990
    ..Patient autoimmune antibodies directed against distinct antigens (RNP, Sm and Me) of the ubiquitous U1 small nuclear ribonucleoprotein particle (U1 snRNP) are strongly associated with connnective tissue diseases, ie...
  2. EUBACTERIA IN PATHOGENESIS OF SYSTEMIC LUPUS ERYTHEMATOSUS
    SHIRLEY KOVACS; Fiscal Year: 2000
    ..disease of unknown etiology but behaves as an antigen-driven response to the nuclear component, U1 small nuclear ribonucleoprotein particle (U1snRNP)...
  3. MECHANISMS OF ANTI-NRNP/SM AUTOANTIBODY SPREADING
    MINOURU SATOH; Fiscal Year: 1999
    The U1 small nuclear ribonucleoprotein (snRNP), which carries Sm as well as nRNP antigenic determinants, is a major target of autoantibodies in human and murine systemic lupus erythematosus (SLE)...
  4. CHARACTERIZATION OF RHEUMATOID ANTIGENS IN CYANOBACTERIA
    SHIRLEY KOVACS; Fiscal Year: 1991
    ..autoantibodies directed against distinct antigenic sites (Sm and RNP respectively) on the ubiquitous U1 small nuclear ribonucleoprotein particle (U1snRNP or U1RNP) of eucaryotic cells...
  5. MECHANISMS OF AUTOREACTIVITY IN SLE
    Joseph Craft; Fiscal Year: 1993
    A central focus of the immune response in systemic lupus erythematosus is the U1 small nuclear ribonucleoprotein particle (U1 snRNP). Both anti-Sm and anti-U1 RNP antibodies, markers for the diagnosis of SLB, target this snRNP...
  6. Immune Response to Small Nuclear Ribonucleoprotein Autoantigens
    Eric Greidinger; Fiscal Year: 2009
    ..Our research goal is to develop new and effective treatments of these disorders. ..
  7. APOPTOSIS-MODIFIED SELF ANTIGEN IN RHEUMATIC DISEASE
    Eric Greidinger; Fiscal Year: 2002
    ..In the future, this work may lead to the development of specific immunotherapy strategies for the treatment of Ul-70kDa-associated rheumatic disorders. ..
  8. Molecular Dynamics of Induced Fit in U1A-RNA Complexes
    BETHANY KORMOS; Fiscal Year: 2006
    ..abstract_text> ..
  9. Immune Response to Small Nuclear Ribonucleoprotein Autoantigens
    Eric L Greidinger; Fiscal Year: 2010
    ..Our research goal is to develop new and effective treatments of these disorders. ..
  10. Epigenetic Control of Adipogenesis
    ANTHONY IMBALZANO; Fiscal Year: 2009
    ..abstract_text> ..
  11. Structure and Function of Copper Transporters
    Vinzenz Unger; Fiscal Year: 2007
    ..Using the substituted cysteine accessibility method and alanine scanning mutagenesis, we will test this hypothesis by determining how the different transmembrane helices contribute to such a copper uptake. ..
  12. TRANSCRIPTION FACTOR FUNCTION IN CHROMATIN
    ANTHONY IMBALZANO; Fiscal Year: 2007
    ....
  13. Regulation of mRNA Splicing by Mer1p
    Marc Spingola; Fiscal Year: 2007
    ..This project will elucidate how Mer1p and similar proteins function and will offer important insights towards the basis of diseases like Fragile X Syndrome in humans. [unreadable] [unreadable] [unreadable]..
  14. Mechanisms of MeCP2 gene expression regulation
    CAROL LUTZ; Fiscal Year: 2007
    ..These studies represent an under-explored area of research on MeCP2 gene expression that may have a critical influence in the tissue-specific effects observed in Rett syndrome. [unreadable] [unreadable] [unreadable]..
  15. 3' end formation of human type I and II collagen mRNAs
    CAROL LUTZ; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  16. Role of G protein Coupling in Fe(ll)-Uptake in Bacteria
    Vinzenz Unger; Fiscal Year: 2006
    ..The fourth aim is to generate crystals of FeoB embedded in a lipid bilayer. Ultimately, this will allow visualization of FeoB, and reveal whether its structure is related to other G protein-coupled membrane proteins. ..
  17. Structure and Function of Copper Transporters
    Vinzenz Unger; Fiscal Year: 2006
    ..Using the substituted cysteine accessibility method and alanine scanning mutagenesis, we will test this hypothesis by determining how the different transmembrane helices contribute to such a copper uptake. ..
  18. Spatial changes in gene organization during adipogenesis
    ANTHONY IMBALZANO; Fiscal Year: 2007
    ..Such molecules may represent an entirely new class of targets for future therapies. [unreadable] [unreadable] [unreadable]..
  19. Epigenetic Control of Adipogenesis
    SAID SIF; Fiscal Year: 2010
    ..abstract_text> ..
  20. Structural Biology of Presynaptic Scaffolds
    Vinzenz Unger; Fiscal Year: 2008
    ....
  21. Mechanisms of MeCP2 gene expression regulation
    CAROL LUTZ; Fiscal Year: 2008
    ..These studies represent an under-explored area of research on MeCP2 gene expression that may have a critical influence in the tissue-specific effects observed in Rett syndrome. [unreadable] [unreadable] [unreadable]..
  22. TRANSCRIPTION FACTOR FUNCTION IN CHROMATIN
    ANTHONY IMBALZANO; Fiscal Year: 2009
    ....
  23. Structure and Function of Copper Transporters
    Vinzenz Unger; Fiscal Year: 2009
    ..Using the substituted cysteine accessibility method and alanine scanning mutagenesis, we will test this hypothesis by determining how the different transmembrane helices contribute to such a copper uptake. ..
  24. Structure and Function of Copper Transporters
    Vinzenz Unger; Fiscal Year: 2008
    ..Using the substituted cysteine accessibility method and alanine scanning mutagenesis, we will test this hypothesis by determining how the different transmembrane helices contribute to such a copper uptake. ..
  25. TRANSCRIPTION FACTOR FUNCTION IN CHROMATIN
    ANTHONY IMBALZANO; Fiscal Year: 2009
    ....
  26. TRANSCRIPTION FACTOR FUNCTION IN CHROMATIN
    ANTHONY IMBALZANO; Fiscal Year: 2008
    ....
  27. TRANSCRIPTION FACTOR FUNCTION IN CHROMATIN
    Anthony N Imbalzano; Fiscal Year: 2010
    ....
  28. Spatial changes in gene organization during adipogenesis
    ANTHONY IMBALZANO; Fiscal Year: 2008
    ..Such molecules may represent an entirely new class of targets for future therapies. [unreadable] [unreadable] [unreadable]..
  29. Structure and Function of Copper Transporters
    Vinzenz Unger; Fiscal Year: 2005
    ..Using the substituted cysteine accessibility method and alanine scanning mutagenesis, we will test this hypothesis by determining how the different transmembrane helices contribute to such a copper uptake. ..
  30. Role of G protein Coupling in Fe(ll)-Uptake in Bacteria
    Vinzenz Unger; Fiscal Year: 2005
    ..The fourth aim is to generate crystals of FeoB embedded in a lipid bilayer. Ultimately, this will allow visualization of FeoB, and reveal whether its structure is related to other G protein-coupled membrane proteins. ..
  31. Role of G protein Coupling in Fe(ll)-Uptake in Bacteria
    Vinzenz Unger; Fiscal Year: 2002
    ..The fourth aim is to generate crystals of FeoB embedded in a lipid bilayer. Ultimately, this will allow visualization of FeoB, and reveal whether its structure is related to other G protein-coupled membrane proteins. ..
  32. PROTEIN ARGININE METHYLATION AND RNA MATURATION
    Michael Henry; Fiscal Year: 2002
    ..A better understanding of arginine methylation should not only enhance the understanding of cellular RNA maturation, but also provide insights into viral mechanisms for RNA maturation during cell infection. ..
  33. TRANSCRIPTION FACTOR FUNCTION IN CHROMATIN
    ANTHONY IMBALZANO; Fiscal Year: 2001
    ....
  34. PROTEIN ARGININE METHYLATION AND RNA MATURATION
    Michael Henry; Fiscal Year: 2001
    ..A better understanding of arginine methylation should not only enhance the understanding of cellular RNA maturation, but also provide insights into viral mechanisms for RNA maturation during cell infection. ..
  35. TRANSCRIPTION FACTOR FUNCTION IN CHROMATIN
    ANTHONY IMBALZANO; Fiscal Year: 2000
    ....
  36. PROTEIN ARGININE METHYLATION AND RNA MATURATION
    Michael Henry; Fiscal Year: 2000
    ..A better understanding of arginine methylation should not only enhance the understanding of cellular RNA maturation, but also provide insights into viral mechanisms for RNA maturation during cell infection. ..
  37. TRANSCRIPTION FACTOR FUNCTION IN CHROMATIN
    ANTHONY IMBALZANO; Fiscal Year: 1999
    ....
  38. PROTEIN ARGININE METHYLATION AND RNA MATURATION
    Michael Henry; Fiscal Year: 1999
    ..A better understanding of arginine methylation should not only enhance the understanding of cellular RNA maturation, but also provide insights into viral mechanisms for RNA maturation during cell infection. ..
  39. TRANSCRIPTION FACTOR FUNCTION IN CHROMATIN
    ANTHONY IMBALZANO; Fiscal Year: 2002
    ..Aim 3 will probe the generality of the requirement for SWI/SNF enzymes in different differentiation pathways and embryogenesis. ..
  40. TRANSCRIPTION FACTOR FUNCTION IN CHROMATIN
    ANTHONY IMBALZANO; Fiscal Year: 2003
    ..Aim 3 will probe the generality of the requirement for SWI/SNF enzymes in different differentiation pathways and embryogenesis. ..
  41. TRANSCRIPTION FACTOR FUNCTION IN CHROMATIN
    ANTHONY IMBALZANO; Fiscal Year: 2005
    ..Aim 3 will probe the generality of the requirement for SWI/SNF enzymes in different differentiation pathways and embryogenesis. ..
  42. TRANSCRIPTION FACTOR FUNCTION IN CHROMATIN
    ANTHONY IMBALZANO; Fiscal Year: 2004
    ..Aim 3 will probe the generality of the requirement for SWI/SNF enzymes in different differentiation pathways and embryogenesis. ..
  43. Role of arginine deficiency in pathogenesis of lupus
    Minoru Satoh; Fiscal Year: 2004
    ..abstract_text> ..
  44. Role of G protein Coupling in Fe(ll)-Uptake in Bacteria
    Vinzenz Unger; Fiscal Year: 2004
    ..The fourth aim is to generate crystals of FeoB embedded in a lipid bilayer. Ultimately, this will allow visualization of FeoB, and reveal whether its structure is related to other G protein-coupled membrane proteins. ..
  45. Tools for Membrane Protein Structure Determination
    Vinzenz Unger; Fiscal Year: 2004
    ..This approach may make a large number of membrane proteins amenable to 2D-crystallization and structure determination. ..
  46. TRANSCRIPTION FACTOR FUNCTION IN CHROMATIN
    ANTHONY IMBALZANO; Fiscal Year: 2004
    ..Aim 3 will probe the generality of the requirement for SWI/SNF enzymes in different differentiation pathways and embryogenesis. ..
  47. Role of G protein Coupling in Fe(ll)-Uptake in Bacteria
    Vinzenz Unger; Fiscal Year: 2003
    ..The fourth aim is to generate crystals of FeoB embedded in a lipid bilayer. Ultimately, this will allow visualization of FeoB, and reveal whether its structure is related to other G protein-coupled membrane proteins. ..
  48. Mechanisms of post-transcriptional regulation of MeCP2
    CAROL LUTZ; Fiscal Year: 2003
    ..These studies represent under-explored areas of MeCP2 expression that may have a critical influence in the tissue-specific effects observed in Rett syndrome. ..
  49. PROTEIN ARGININE METHYLATION AND RNA MATURATION
    Michael Henry; Fiscal Year: 2003
    ..A better understanding of arginine methylation should not only enhance the understanding of cellular RNA maturation, but also provide insights into viral mechanisms for RNA maturation during cell infection. ..