u1 small nuclear ribonucleoprotein

Summary

Summary: A nuclear RNA-protein complex that plays a role in RNA processing. In the nucleoplasm, the U1 snRNP along with other small nuclear ribonucleoproteins (U2, U4-U6, and U5) assemble into SPLICEOSOMES that remove introns from pre-mRNA by splicing. The U1 snRNA forms base pairs with conserved sequence motifs at the 5'-splice site and recognizes both the 5'- and 3'-splice sites and may have a fundamental role in aligning the two sites for the splicing reaction.

Top Publications

  1. ncbi An RNA map predicting Nova-dependent splicing regulation
    Jernej Ule
    Howard Hughes Medical Institute, New York, New York 10021, USA
    Nature 444:580-6. 2006
  2. ncbi Heterogeneous nuclear ribonucleoproteins H and F regulate the proteolipid protein/DM20 ratio by recruiting U1 small nuclear ribonucleoprotein through a complex array of G runs
    Erming Wang
    Department of Neurology, University of Kentucky, Lexington, Kentucky 40536, USA
    J Biol Chem 284:11194-204. 2009
  3. ncbi In vitro reconstitution of mammalian U1 snRNPs active in splicing: the U1-C protein enhances the formation of early (E) spliceosomal complexes
    C L Will
    Institut fur Molekularbiologie und Tumorforschung, Philipps Universitat Marburg, Germany
    Nucleic Acids Res 24:4614-23. 1996
  4. ncbi The apoptosis-promoting factor TIA-1 is a regulator of alternative pre-mRNA splicing
    P Förch
    Gene Expression Programme European Molecular Biology Laboratory Meyerhofstrasse 1 D 69117, Heidelberg, Germany
    Mol Cell 6:1089-98. 2000
  5. ncbi Interaction of the U1 snRNP with nonconserved intronic sequences affects 5' splice site selection
    O Puig
    Gene Expression Program, European Molecular Biology Laboratory, D 69117 Heidelberg, Germany
    Genes Dev 13:569-80. 1999
  6. ncbi Sequence-specific interaction of U1 snRNA with the SMN complex
    Jeongsik Yong
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6148, USA
    EMBO J 21:1188-96. 2002
  7. ncbi The NMR structure of the 38 kDa U1A protein - PIE RNA complex reveals the basis of cooperativity in regulation of polyadenylation by human U1A protein
    L Varani
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Nat Struct Biol 7:329-35. 2000
  8. ncbi The fate of U1 snRNP during anti-Fas induced apoptosis: specific cleavage of the U1 snRNA molecule
    W G Degen
    Department of Biochemistry, University of Nijmegen, NL 6500 HB Nijmegen, The Netherlands
    Cell Death Differ 7:70-9. 2000
  9. ncbi The U1 snRNP base pairs with the 5' splice site within a penta-snRNP complex
    Hadar Malca
    Department of Human Genetics and Molecular Medicine, Sackler School of Medicine, Tel Aviv University, Ramat Aviv 69978, Tel Aviv, Israel
    Mol Cell Biol 23:3442-55. 2003
  10. ncbi An SC35-like protein and a novel serine/arginine-rich protein interact with Arabidopsis U1-70K protein
    M Golovkin
    Department of Biology and Program in Cell and Molecular Biology, Colorado State University, Fort Collins, Colorado 80523, USA
    J Biol Chem 274:36428-38. 1999

Research Grants

  1. Immune Response to Small Nuclear Ribonucleoprotein Autoantigens
    Eric Greidinger; Fiscal Year: 2009
  2. APOPTOSIS-MODIFIED SELF ANTIGEN IN RHEUMATIC DISEASE
    Eric Greidinger; Fiscal Year: 2002
  3. Molecular Dynamics of Induced Fit in U1A-RNA Complexes
    BETHANY KORMOS; Fiscal Year: 2006
  4. Immune Response to Small Nuclear Ribonucleoprotein Autoantigens
    Eric L Greidinger; Fiscal Year: 2010
  5. Regulation of mRNA Splicing by Mer1p
    Marc Spingola; Fiscal Year: 2007
  6. Mechanisms of MeCP2 gene expression regulation
    CAROL LUTZ; Fiscal Year: 2007
  7. 3' end formation of human type I and II collagen mRNAs
    CAROL LUTZ; Fiscal Year: 2007
  8. Role of G protein Coupling in Fe(ll)-Uptake in Bacteria
    Vinzenz Unger; Fiscal Year: 2006
  9. Spatial changes in gene organization during adipogenesis
    ANTHONY IMBALZANO; Fiscal Year: 2007
  10. Epigenetic Control of Adipogenesis
    SAID SIF; Fiscal Year: 2010

Detail Information

Publications165 found, 100 shown here

  1. ncbi An RNA map predicting Nova-dependent splicing regulation
    Jernej Ule
    Howard Hughes Medical Institute, New York, New York 10021, USA
    Nature 444:580-6. 2006
    ....
  2. ncbi Heterogeneous nuclear ribonucleoproteins H and F regulate the proteolipid protein/DM20 ratio by recruiting U1 small nuclear ribonucleoprotein through a complex array of G runs
    Erming Wang
    Department of Neurology, University of Kentucky, Lexington, Kentucky 40536, USA
    J Biol Chem 284:11194-204. 2009
    ..Binding of U1 small nuclear ribonucleoprotein (U1snRNP) to DM20 is greatly impaired by mutation of G1 and M2 and depletion of hnRNPH and F...
  3. ncbi In vitro reconstitution of mammalian U1 snRNPs active in splicing: the U1-C protein enhances the formation of early (E) spliceosomal complexes
    C L Will
    Institut fur Molekularbiologie und Tumorforschung, Philipps Universitat Marburg, Germany
    Nucleic Acids Res 24:4614-23. 1996
    ..Studies with recombinant U1-C protein mutants indicated that the N-terminal domain of U1-C is necessary and sufficient for the stimulation of E complex formation...
  4. ncbi The apoptosis-promoting factor TIA-1 is a regulator of alternative pre-mRNA splicing
    P Förch
    Gene Expression Programme European Molecular Biology Laboratory Meyerhofstrasse 1 D 69117, Heidelberg, Germany
    Mol Cell 6:1089-98. 2000
    ....
  5. ncbi Interaction of the U1 snRNP with nonconserved intronic sequences affects 5' splice site selection
    O Puig
    Gene Expression Program, European Molecular Biology Laboratory, D 69117 Heidelberg, Germany
    Genes Dev 13:569-80. 1999
    ..This supports a model where early 5' splice recognition results from a network of interactions established by the splicing machinery with various regions of the pre-mRNA...
  6. ncbi Sequence-specific interaction of U1 snRNA with the SMN complex
    Jeongsik Yong
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6148, USA
    EMBO J 21:1188-96. 2002
    ..These findings indicate that SMN complex interaction with SL1 is sequence-specific and critical for U1 snRNP biogenesis, further supporting the direct role of the SMN complex in RNP biogenesis...
  7. ncbi The NMR structure of the 38 kDa U1A protein - PIE RNA complex reveals the basis of cooperativity in regulation of polyadenylation by human U1A protein
    L Varani
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Nat Struct Biol 7:329-35. 2000
    ..This mechanism ensures that the activity of PAP enzyme, which is essential to the cell, is only down regulated when U1A is bound to the U1A mRNA...
  8. ncbi The fate of U1 snRNP during anti-Fas induced apoptosis: specific cleavage of the U1 snRNA molecule
    W G Degen
    Department of Biochemistry, University of Nijmegen, NL 6500 HB Nijmegen, The Netherlands
    Cell Death Differ 7:70-9. 2000
    ....
  9. ncbi The U1 snRNP base pairs with the 5' splice site within a penta-snRNP complex
    Hadar Malca
    Department of Human Genetics and Molecular Medicine, Sackler School of Medicine, Tel Aviv University, Ramat Aviv 69978, Tel Aviv, Israel
    Mol Cell Biol 23:3442-55. 2003
    ..This fraction is functional in mRNA spliceosome assembly when supplemented with soluble nuclear proteins. The results argue that U1 can bind the 5' splice site in a mammalian preassembled penta-snRNP complex...
  10. ncbi An SC35-like protein and a novel serine/arginine-rich protein interact with Arabidopsis U1-70K protein
    M Golovkin
    Department of Biology and Program in Cell and Molecular Biology, Colorado State University, Fort Collins, Colorado 80523, USA
    J Biol Chem 274:36428-38. 1999
    The U1 small nuclear ribonucleoprotein 70-kDa protein, a U1 small nuclear ribonucleoprotein-specific protein, has been shown to have multiple roles in nuclear precursor mRNA processing in animals...
  11. ncbi Altering the RNA-binding mode of the U1A RBD1 protein
    Scott A Showalter
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110, USA
    J Mol Biol 335:465-80. 2004
    ..The molecular basis for this alteration in RNA-binding properties is proposed to result from the inability of the RNA to induce a change in the structure of the free protein to produce a high-affinity complex...
  12. ncbi Yeast ortholog of the Drosophila crooked neck protein promotes spliceosome assembly through stable U4/U6.U5 snRNP addition
    S Chung
    T H Morgan School of Biological Sciences and The Markey Cancer Center, University of Kentucky, Lexington 40506 0225, USA
    RNA 5:1042-54. 1999
    ..Our results indicate that Clf1p acts as a scaffolding protein in spliceosome assembly and suggest that Clf1p may support the cross-intron bridge during the prespliceosome-to-spliceosome transition...
  13. ncbi Dephosphorylated SRp38 acts as a splicing repressor in response to heat shock
    Chanseok Shin
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    Nature 427:553-8. 2004
    ..We further show that dephosphorylated SRp38 interacts with a U1 small nuclear ribonucleoprotein particle (snRNP) protein, and that this interaction interferes with 5'-splice-site recognition by ..
  14. ncbi An intronic polypyrimidine-rich element downstream of the donor site modulates cystic fibrosis transmembrane conductance regulator exon 9 alternative splicing
    Elisabetta Zuccato
    International Centre for Genetic Engineering and Biotechnology, Padriciano 99, Trieste 34012, Italy
    J Biol Chem 279:16980-8. 2004
    ..exon 9 inclusion, consistent with the facilitating role of TIA-1 in weak 5'-ss recognition by U1 small nuclear ribonucleoprotein. Interestingly, in the presence of a high number of TG repeats and a low number of T repeats in the ..
  15. ncbi Human monoclonal autoantibody fragments from combinatorial antibody libraries directed to the U1snRNP associated U1C protein; epitope mapping, immunolocalization and V-gene usage
    R M Hoet
    Department of Biochemistry, University of Nijmegen, The Netherlands
    Mol Immunol 35:1045-55. 1998
    ..The two cross-reactive antibodies immunoprecipitate the other Sm containing snRNPs as well. Using confocal immunofluorescence microscopy we could show that the major part of the U1C protein is localized within the coiled body structure...
  16. ncbi The yeast splicing factor Prp40p contains functional leucine-rich nuclear export signals that are essential for splicing
    Mark W Murphy
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Genetics 166:53-65. 2004
    ....
  17. ncbi Crystal structure of the spliceosomal U2B"-U2A' protein complex bound to a fragment of U2 small nuclear RNA
    S R Price
    MRC Laboratory of Molecular Biology, Cambridge, UK
    Nature 394:645-50. 1998
    ....
  18. ncbi A serine/arginine-rich domain in the human U1 70k protein is necessary and sufficient for ASF/SF2 binding
    W Cao
    Department of Pharmacology and Cancer Biology, Levine Science Research Center, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 273:20629-35. 1998
    ..ASF/SF2, SC35, and other members of the serine/arginine family, interact with the 70k protein of the U1 small nuclear ribonucleoprotein. The binding of this protein with ASF/SF2 is thought to enhance recognition of the 5' splice site of ..
  19. ncbi Nuclear accumulation of the U1 snRNP-specific protein C is due to diffusion and retention in the nucleus
    J M Klein Gunnewiek
    Department of Biochemistry, University of Nijmegen, Nijmegen, 6500 HB, The Netherlands
    Exp Cell Res 235:265-73. 1997
    The U1 small nuclear ribonucleoprotein particle (snRNP) has an important function in the early formation of the spliceosome, the multicomponent complex in which pre-mRNA splicing takes place...
  20. ncbi A functional role for correlated motion in the N-terminal RNA-binding domain of human U1A protein
    Scott A Showalter
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 South Euclid, Box 8231, 63110, St Louis, MO, USA
    J Mol Biol 322:533-42. 2002
    ..Interpretation of the novel RED results and corresponding NMR relaxation data suggests that the loss of collective motions in the mutants could account for their weak RNA-binding...
  21. ncbi Determinants within an 18-amino-acid U1A autoregulatory domain that uncouple cooperative RNA binding, inhibition of polyadenylation, and homodimerization
    Fei Guan
    Rutgers University, Piscataway, New Jersey 08854, USA
    Mol Cell Biol 23:3163-72. 2003
    ..Our molecular dissection of the consequences of conformational changes within an RNP complex presents a powerful example to those studying more complicated pre-mRNA-regulatory systems...
  22. ncbi Antinuclear antibodies recognize cellular autoantigens driven by apoptosis
    Roxana Ramírez-Sandoval
    Department of Molecular Biology, Centro de Biología Experimental, Universidad Autonoma de Zacatecas, Chepinque 306, Col Lomas de la Soledad, 98040, Zacatecas, Mexico
    Joint Bone Spine 70:187-94. 2003
    ..Present study addresses the issue whether cellular antigens recognised by antinuclear autoantibodies are driven by apoptosis...
  23. ncbi An intronic splicing enhancer binds U1 snRNPs to enhance splicing and select 5' splice sites
    A J McCullough
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Mol Cell Biol 20:9225-35. 2000
    ..We suggest that the G-rich region near vertebrate 5' splice sites promotes accurate splice site recognition by recruiting the U1 snRNP...
  24. ncbi The fate of the U1 snRNP autoantigen during apoptosis: implications for systemic autoimmunity
    Kelen C R Malmegrim
    Department of Biochemistry, Nijmegen Center of Molecular Life Sciences, University of Nijmegen, Nijmegen, The Netherlands
    Isr Med Assoc J 4:706-12. 2002
    ....
  25. ncbi A multiprotein complex mediates the ATP-dependent assembly of spliceosomal U snRNPs
    G Meister
    Max-Planck Institute of Biochemistry, Am Klopferspitz 18a, D-82152 Martinsried, Germany
    Nat Cell Biol 3:945-9. 2001
    ..These data provide the first direct evidence that a complex containing SMN and Gemin2 mediates the active assembly of spliceosomal U snRNPs...
  26. ncbi Secondary necrosis is a source of proteolytically modified forms of specific intracellular autoantigens: implications for systemic autoimmunity
    X Wu
    Loma Linda University School of Medicine, California, USA
    Arthritis Rheum 44:2642-52. 2001
    ....
  27. ncbi Substitution of an essential adenine in the U1A-RNA complex with a non-polar isostere
    Jacob B Tuite
    Department of Chemistry, Wesleyan University, Middletown, CT 06459, USA
    Nucleic Acids Res 30:5269-75. 2002
    ..The data imply that hydrophobic interactions can compensate energetically for the disruption of the complex hydrogen-bonding network between nucleotide and protein...
  28. ncbi The Drosophila U2 snRNP protein U2A' has an essential function that is SNF/U2B" independent
    A A Nagengast
    Department of Genetics, Case Western Reserve University, School of Medicine, 10900 Euclid Avenue, Cleveland, OH 44106 4955, USA
    Nucleic Acids Res 29:3841-7. 2001
    ..Thus together these data demonstrate that in Drosophila U2A' has an essential function that is unrelated to its role as the partner protein of SNF/U2B"...
  29. ncbi Modulation of P-element pre-mRNA splicing by a direct interaction between PSI and U1 snRNP 70K protein
    E Labourier
    Department of Molecular and Cell Biology, University of California, Berkeley, 401 Barker Hall 3204, Berkeley, CA 94720, USA
    Mol Cell 8:363-73. 2001
    ..Here we have identified a direct association of PSI with the spliceosomal U1 small nuclear ribonucleoprotein (snRNP) particle in somatic nuclear extracts...
  30. ncbi Autoimmune response to U1 small nuclear ribonucleoprotein (U1 snRNP) associated with cytomegalovirus infection
    M M Newkirk
    Division of Rheumatology, The Montreal General Hospital, McGill University, Montreal, Quebec, Canada
    Arthritis Res 3:253-8. 2001
    The induction of autoantibodies to U1 small nuclear ribonucleoprotein (U1 snRNP) complexes is not well understood...
  31. ncbi Spliceosomal UsnRNP biogenesis, structure and function
    C L Will
    Max Planck Institute of Biophysical Chemistry, Department of Cellular Biochemistry, Am Fassberg 11, 37077 Gottingen, Germany
    Curr Opin Cell Biol 13:290-301. 2001
    ....
  32. ncbi A general role for splicing enhancers in exon definition
    Bianca J Lam
    Department of Microbiology and Molecular Genetics, College of Medicine, University of California, Irvine 92697-4025, USA
    RNA 8:1233-41. 2002
    ..Our results suggest that ESEs recruit a multicomponent complex that minimally contains components of the splicing machinery required for 5' and 3' splice site selection...
  33. ncbi The appearance of U1 RNP antibody specificities in sequential autoimmune human antisera follows a characteristic order that implicates the U1-70 kd and B'/B proteins as predominant U1 RNP immunogens
    E L Greidinger
    University of Missouri, Columbia, USA
    Arthritis Rheum 44:368-75. 2001
    ..CONCLUSION: Orderly patterns of emergence of U1 RNP peptide antibodies appear to exist in humans. Two peptides, 70 kd and B'/B, show characteristics of early immunogens in the development of human RNP immunity...
  34. ncbi Sm protein-Sm site RNA interactions within the inner ring of the spliceosomal snRNP core structure
    H Urlaub
    Max Planck Institute of Biophysical Chemistry, Department of Cellular Biochemistry, Am Fassberg 11, D 37077 Gottingen, Germany
    EMBO J 20:187-96. 2001
    ..Our results thus provide the first evidence that, within the core snRNP, multiple Sm protein-Sm site RNA contacts occur on the inner surface of the heptameric Sm protein ring...
  35. ncbi Yeast U1 snRNP-pre-mRNA complex formation without U1snRNA-pre-mRNA base pairing
    H Du
    Department of Biology, Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02454, USA
    RNA 7:133-42. 2001
    ..Only one of these is base pairing between the 5' splice site and the 5' end of U1 snRNA, without which the U1 snRNP-pre-mRNA complex is less stable and has a somewhat altered conformation...
  36. ncbi Specific alterations of U1-C protein or U1 small nuclear RNA can eliminate the requirement of Prp28p, an essential DEAD box splicing factor
    J Y Chen
    Department of Molecular Genetics, Ohio State University, Columbus, OH 43210, USA
    Mol Cell 7:227-32. 2001
    ..to counteract the stabilizing effect of the U1-C protein, thereby promoting the dissociation of the U1 small nuclear ribonucleoprotein particle from the 5' splice site...
  37. ncbi Sex-lethal splicing autoregulation in vivo: interactions between SEX-LETHAL, the U1 snRNP and U2AF underlie male exon skipping
    Alexis A Nagengast
    Department of Genetics, Case Western Reserve University, Cleveland, OH 44106 4955, USA
    Development 130:463-71. 2003
    ....
  38. ncbi Apoptotic U1-70 kd is antigenically distinct from the intact form of the U1-70-kd molecule
    Eric L Greidinger
    University of Missouri and Department of Veterans Affairs Medical Research Service, Harry S Truman Memorial Veterans Hospital, Columbia, Missouri, USA
    Arthritis Rheum 46:1264-9. 2002
    ..To determine whether immune responses to an apoptotically modified form of a human lupus autoantigen can be distinguished from immune responses to the intact form of the same antigen...
  39. ncbi U1A inhibits cleavage at the immunoglobulin M heavy-chain secretory poly(A) site by binding between the two downstream GU-rich regions
    Catherine Phillips
    Molecular Biology and Biochemistry, Rutgers University, Nelson Labs, Room A322, 604 Allison Rd, Piscataway, NJ 08854, USA
    Mol Cell Biol 24:6162-71. 2004
    ..We demonstrate here that U1A binds two (AUGCN(1-3)C) motifs within the 29-nucleotide sequence and inhibits the binding of cleavage stimulatory factor 64K and cleavage at the secretory poly(A) site...
  40. ncbi Structure and expression of a plant U1 snRNP 70K gene: alternative splicing of U1 snRNP 70K pre-mRNAs produces two different transcripts
    M Golovkin
    Department of Biology, Colorado State University, Fort Collins 80523, USA
    Plant Cell 8:1421-35. 1996
    The product of the U1 small nuclear ribonucleoprotein particle (U1 snRNP) 70K (U1-70K) gene, a U1 snRNP-specific protein, has been implicated in basic as well as alternative splicing of pre-mRNAs in animals...
  41. ncbi Protein-protein interactions and 5'-splice-site recognition in mammalian mRNA precursors
    J D Kohtz
    Department of Biological Sciences, Columbia University, New York, New York 10027
    Nature 368:119-24. 1994
    ..This cooperation is probably achieved by specific interactions between the arginine/serine-rich domain of the splicing factor and a similar region in a U1 snRNP-specific protein...
  42. ncbi The association of the U1-specific 70K and C proteins with U1 snRNPs is mediated in part by common U snRNP proteins
    R L Nelissen
    Department of Biochemistry, University of Nijmegen, The Netherlands
    EMBO J 13:4113-25. 1994
    The U1 small nuclear ribonucleoprotein particle (snRNP)-specific 70K and A proteins are known to bind directly to stem-loops of the U1 snRNA, whereas the U1-C protein does not bind to naked U1 snRNA, but depends on other U1 snRNP protein ..
  43. ncbi Molecular characterization of the spliceosomal proteins U1A and U2B" from higher plants
    G G Simpson
    Cell and Molecular Genetics Department, Scottish Crop Research Institute, Invergowrie, Dundee, UK
    EMBO J 14:4540-50. 1995
    ....
  44. ncbi Complete MALDI-ToF MS analysis of cross-linked peptide-RNA oligonucleotides derived from nonlabeled UV-irradiated ribonucleoprotein particles
    Eva Kühn-Hölsken
    Bioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry, 37077 Gottingen, Germany
    RNA 11:1915-30. 2005
    ..This type of analysis is applicable to any type of RNP complex and may be expected to pave the way for the further analysis of protein-RNA complexes in much lower abundance and/or of cross-links that are obtained in low yield...
  45. ncbi Crystal structure at 1.92 A resolution of the RNA-binding domain of the U1A spliceosomal protein complexed with an RNA hairpin
    C Oubridge
    MRC Laboratory of Molecular Biology, Cambridge, UK
    Nature 372:432-8. 1994
    ..The structure reveals the stereochemical basis for sequence-specific RNA recognition by the RNP domain...
  46. ncbi The Drosophila sex determination gene snf encodes a nuclear protein with sequence and functional similarity to the mammalian U1A snRNP protein
    T W Flickinger
    Department of Genetics, Case Western Reserve University, Cleveland, Ohio 44106 4955
    Genes Dev 8:914-25. 1994
    ..These studies provide the first demonstration, in a multicellular organism, that mutations in a U1 snRNP protein alter splicing in vivo...
  47. ncbi Nucleo-cytoplasmic transport of U snRNPs: definition of a nuclear location signal in the Sm core domain that binds a transport receptor independently of the m3G cap
    U Fischer
    , , Germany
    EMBO J 12:573-83. 1993
    ..We propose that the RNA structure of a given snRNP particle determines at least in part whether the particle's m3G cap is required for nuclear transport or can be dispensed with...
  48. ncbi Structural basis for RanGTP independent entry of spliceosomal U snRNPs into the nucleus
    Daniel Wohlwend
    Abteilung für Molekulare Strukturbiologie, Institut für Mikrobiologie und Genetik and GZMB, Georg August Universitat Gottingen, Justus von Liebig Weg 11, D 37077 Gottingen, Germany
    J Mol Biol 374:1129-38. 2007
    ....
  49. ncbi A new type of mutation causes a splicing defect in ATM
    Franco Pagani
    Molecular Pathology, International Centre for Genetic Engineering and Biotechnology, Trieste, Italy
    Nat Genet 30:426-9. 2002
    ..a new intron-splicing processing element (ISPE) complementary to U1 snRNA, the RNA component of the U1 small nuclear ribonucleoprotein (snRNP)...
  50. ncbi Non-snRNP U1A levels decrease during mammalian B-cell differentiation and release the IgM secretory poly(A) site from repression
    Jianglin Ma
    Rutgers University, Department of Molecular Biology and Biochemistry, Nelson Laboratories, Room A322, 604 Allison Road, Piscataway, NJ 08854, USA
    RNA 12:122-32. 2006
    ....
  51. ncbi A murine model of mixed connective tissue disease induced with U1 small nuclear RNP autoantigen
    Eric L Greidinger
    Division of Rheumatology and Immunology, University of Miami, Miami, Florida 33136, USA
    Arthritis Rheum 54:661-9. 2006
    ..To test whether immunizing mice with autoantigens closely linked to mixed connective tissue disease (MCTD) could induce an MCTD-like clinical syndrome distinguishable from systemic lupus erythematosus (SLE)...
  52. ncbi Proteomic analysis of the U1 snRNP of Schizosaccharomyces pombe reveals three essential organism-specific proteins
    Alain N S Newo
    Institute of Genetics, Technical University of Braunschweig, Spielmannstr 7, 38106 Braunschweig, Germany
    Nucleic Acids Res 35:1391-401. 2007
    ..pombe are essential for growth. We will discuss the differences between the U1 snRNPs with respect to the organism-specific proteins found in the two yeasts and the resulting effect it has on pre-mRNA splicing...
  53. ncbi Isolation of S. cerevisiae snRNPs: comparison of U1 and U4/U6.U5 to their human counterparts
    P Fabrizio
    Institut fur Molekularbiologie und Tumorforschung, Phillipps Universität, Marburg, Germany
    Science 264:261-5. 1994
    ..U5 snRNPs are significantly similar. The preparative isolation of yeast snRNPs will allow the cloning as well as genetic and phylogenetic analysis of snRNP proteins which will accelerate our understanding of their function...
  54. ncbi Commitment of yeast pre-mRNA to the splicing pathway requires a novel U1 small nuclear ribonucleoprotein polypeptide, Prp39p
    S R Lockhart
    T H Morgan School of Biological Sciences, University of Kentucky, Lexington 40506 0225
    Mol Cell Biol 14:3623-33. 1994
    The binding of a U1 small nuclear ribonucleoprotein (snRNP) particle to the 5' splice site region of a pre-mRNA is a primary step of intron recognition...
  55. ncbi Apoptotic modifications affect the autoreactivity of the U1 snRNP autoantigen
    D Hof
    Department of Biochemistry, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen, Nijmegen, The Netherlands
    Autoimmun Rev 4:380-8. 2005
    ....
  56. ncbi hnRNP H binding at the 5' splice site correlates with the pathological effect of two intronic mutations in the NF-1 and TSHbeta genes
    Emanuele Buratti
    International Centre for Genetic Engineering and Biotechnology, 34012 Trieste, Italy
    Nucleic Acids Res 32:4224-36. 2004
    ..Depletion analyses shows that this protein restricts the accessibility of U1 small nuclear ribonucleoprotein (U1snRNA) to the donor site...
  57. ncbi Assembly and localization of the U1-specific snRNP C protein in the amphibian oocyte
    M F Jantsch
    Department of Embryology, Carnegie Institution, Baltimore, Maryland 21210
    J Cell Biol 119:1037-46. 1992
    ..In transfected tissue culture cells, the myc-tagged C protein localized within the nucleus in a speckled pattern similar to that of endogenous U1 snRNPs...
  58. ncbi Co-localisation studies of Arabidopsis SR splicing factors reveal different types of speckles in plant cell nuclei
    Zdravko J Lorkovic
    Max F Perutz Laboratories, Medical University of Vienna, Department of Medical Biochemistry, Vienna, Austria
    Exp Cell Res 314:3175-86. 2008
    ....
  59. ncbi Autoantibodies specific for apoptotic U1-70K are superior serological markers for mixed connective tissue disease
    Danielle Hof
    Department of Biochemistry, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen, Nijmegen, The Netherlands
    Arthritis Res Ther 7:R302-9. 2005
    ..Patients suffering from mixed connective tissue disease (MCTD) produce autoantibodies directed to U1 small nuclear ribonucleoprotein (snRNP), and antibodies against a 70 kDa protein component, the U1-70K (70K) protein, are the most ..
  60. ncbi U1 small nuclear RNP from Trypanosoma brucei: a minimal U1 snRNA with unusual protein components
    Zsofia Palfi
    , , D-35392 Giessen, Germany
    Nucleic Acids Res 33:2493-503. 2005
    ..These data result in a model of the trypanosome U1 snRNP, which deviates substantially from our classical view of the U1 particle and may reflect the special requirements for splicing of a small set of cis-introns in trypanosomes...
  61. ncbi Solution structure of the N-terminal RNP domain of U1A protein: the role of C-terminal residues in structure stability and RNA binding
    J M Avis
    MRC Laboratory of Molecular Biology, Cambridge, UK
    J Mol Biol 257:398-411. 1996
    ..The movement of helix C mainly involves changes in the main-chain torsion angles of Thr89, Asp90 and Ser91, the helix thereby acting as a "lid" over the RNA binding surface...
  62. ncbi A study of collective atomic fluctuations and cooperativity in the U1A-RNA complex based on molecular dynamics simulations
    Bethany L Kormos
    Chemistry Department and Molecular Biophysics Program, Wesleyan University, 237 Church St, Middletown, CT 06459, USA
    J Struct Biol 157:500-13. 2007
    ..This method has significant implications as a predictive tool regarding cooperativity in the protein-nucleic acid recognition process...
  63. ncbi Hu antigen R (HuR) functions as an alternative pre-mRNA splicing regulator of Fas apoptosis-promoting receptor on exon definition
    José M Izquierdo
    Departamento de Biología Molecular and the Centro de Biología Molecular Severo Ochoa, C S I C, Universidad Autonoma de Madrid, Cantoblanco 28049, Madrid, Spain
    J Biol Chem 283:19077-84. 2008
    ..Taken together, these results support a functional link between HuR as repressor of alternative Fas splicing and the molecular mechanisms modulating programmed cell death...
  64. ncbi Splicing-independent recruitment of spliceosomal small nuclear RNPs to nascent RNA polymerase II transcripts
    Snehal Bhikhu Patel
    Department of Biochemistry, College of Medicine, School of Molecular and Cellular Biology, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    J Cell Biol 178:937-49. 2007
    ..Collectively, these data indicate that the recruitment of snRNPs to nascent transcripts and the assembly of the spliceosome are uncoupled events...
  65. ncbi SR proteins function in coupling RNAP II transcription to pre-mRNA splicing
    Rita Das
    Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Mol Cell 26:867-81. 2007
    ..Thus, these factors readily out-compete inhibitory hnRNP proteins, resulting in efficient spliceosome assembly on nascent RNAP II transcripts...
  66. ncbi Molecular dynamics simulation studies of induced fit and conformational capture in U1A-RNA binding: do molecular substates code for specificity?
    Felicia Pitici
    Chemistry Department and Molecular Biophysics Program, Wesleyan University, Middletown, CT 06459, USA
    Biopolymers 65:424-35. 2002
    ..7947-7956) and by Williamson (Nature Structural Biology, 2000, Vol. 7, pp. 834-837) suggest an important role for intrinsic molecular architecture and substates other than the native form in the specificity of protein-RNA interactions...
  67. ncbi Phosphorylation switches the general splicing repressor SRp38 to a sequence-specific activator
    Ying Feng
    Department of Biological Sciences, Columbia University, 1212 Amsterdam Avenue, New York, New York 10027, USA
    Nat Struct Mol Biol 15:1040-8. 2008
    ..Together, our data demonstrate that SRp38, in addition to its role as a splicing repressor, can function as an unusual sequence-specific splicing activator...
  68. ncbi The splicing regulator TIA-1 interacts with U1-C to promote U1 snRNP recruitment to 5' splice sites
    Patrik Förch
    Gene Expression Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    EMBO J 21:6882-92. 2002
    The U1 small nuclear ribonucleoprotein (U1 snRNP) binds to the pre-mRNA 5' splice site (ss) at early stages of spliceosome assembly...
  69. ncbi Prediction of salt and mutational effects on the association rate of U1A protein and U1 small nuclear RNA stem/loop II
    Sanbo Qin
    Department of Physics and Institute of Molecular Biophysics and School of Computational Science, Florida State University, Tallahassee, Florida 32306, USA
    J Phys Chem B 112:5955-60. 2008
    ..These observations are rationalized. Moreover, predicted effects of salt and charge mutations are found to be in quantitative agreement with experimental results...
  70. ncbi T cell immunity in connective tissue disease patients targets the RNA binding domain of the U1-70kDa small nuclear ribonucleoprotein
    Eric L Greidinger
    Division of Immunology and Rheumatology, University of Missouri, Columbia, MO 65212, USA
    J Immunol 169:3429-37. 2002
    ..The T cell autoimmune response to U1-70kDa appears to have less diversity than is seen in the humoral response; and therefore, may be a favorable target for therapeutic intervention...
  71. ncbi Snu56p is required for Mer1p-activated meiotic splicing
    Richard J Balzer
    Department of Molecular Biology, UMNDJ SOM, 2 Medical Center Drive, Stratford, NJ 08084, USA
    Mol Cell Biol 28:2497-508. 2008
    ..Interestingly, these two proteins do not interact, suggesting that Snu56p links pre-mRNA-bound Mer1p to Nam8p in the U1 snRNP. This work demonstrates that the Snu56 protein is required for splicing only during meiosis...
  72. ncbi Identification of nuclear spliceosomal antigens targeted by NOD mouse antibodies following sodium iodide intake
    C Thompson
    University of Cambridge, Department of Pathology, Tennis Court Road, Cambridge, CB2 1QP, UK
    Autoimmunity 39:99-106. 2006
    ..The autoantibody isotypes most consistently represented were IgG2a and IgG2b...
  73. ncbi A bidirectional SF2/ASF- and SRp40-dependent splicing enhancer regulates human immunodeficiency virus type 1 rev, env, vpu, and nef gene expression
    Massimo Caputi
    Department of Biology, The Johns Hopkins University, Baltimore, MD 21218, USA
    J Virol 78:6517-26. 2004
    ..4, even when the 5' splice site and the U1 snRNA have been mutated to obtain a perfect complementary match. The ESE characterized here is highly conserved in most viral subtypes...
  74. ncbi Characterization of a U2AF-independent commitment complex (E') in the mammalian spliceosome assembly pathway
    Oliver A Kent
    4-39 Medical Sciences Building, Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7
    Mol Cell Biol 25:233-40. 2005
    ..recognition of pre-mRNA during spliceosome assembly in mammals proceeds through the association of U1 small nuclear ribonucleoprotein particle (snRNP) with the 5' splice site as well as the interactions of the branch binding protein ..
  75. ncbi Use of fluorescent protein tags to study nuclear organization of the spliceosomal machinery in transiently transformed living plant cells
    Zdravko J Lorkovic
    Max F Perutz Laboratories, University Departments at the Vienna Biocenter, Institute of Medical Biochemistry, 1030 Vienna, Austria
    Mol Biol Cell 15:3233-43. 2004
    ....
  76. ncbi Kinetic analysis of the role of the tyrosine 13, phenylalanine 56 and glutamine 54 network in the U1A/U1 hairpin II interaction
    Michael J Law
    Department of Biochemistry and Molecular Biology, University of Southern California Los Angeles, CA 90089-9176, USA
    Nucleic Acids Res 33:2917-28. 2005
    The A protein of the U1 small nuclear ribonucleoprotein particle, interacting with its stem-loop RNA target (U1hpII), is frequently used as a paradigm for RNA binding by recognition motif domains (RRMs)...
  77. ncbi NSSRs/TASRs/SRp38s function as splicing modulators via binding to pre-mRNAs
    Kazuo Fushimi
    Center for Nano Materials and Technology, Japan Advanced Institute of Science and Technology, Nomi City, Ishikawa 923 1292, Japan
    Genes Cells 10:531-41. 2005
    ..5, all containing undifferentiated neural stem cells. Taken together, our results showed that NSSRs modulate alternative splicing via binding to premRNAs during neural differentiation...
  78. ncbi Structural availability influences the capacity of autoantigenic epitopes to induce a widespread lupus-like autoimmune response
    Micah T McClain
    Oklahoma Medical Research Foundation, University of Oklahoma Health Sciences Center, and Veterans Affairs Medical Center, Oklahoma City, OK 73104, USA
    Proc Natl Acad Sci U S A 101:3551-6. 2004
    ....
  79. ncbi Mer1p is a modular splicing factor whose function depends on the conserved U2 snRNP protein Snu17p
    Marc Spingola
    Center for the Molecular Biology of RNA, Sinsheimer Laboratories, University of California, Santa Cruz, Santa Cruz, CA 95064, USA
    Nucleic Acids Res 32:1242-50. 2004
    ..We conclude that Mer1p is a modular splicing regulator that can activate splicing at several early steps of spliceosome assembly and depends on the activities of both U1 and U2 snRNP proteins to activate splicing...
  80. ncbi Suppressors of a cold-sensitive mutation in yeast U4 RNA define five domains in the splicing factor Prp8 that influence spliceosome activation
    A N Kuhn
    Department of Biomolecular Chemistry, University of Wisconsin Medical School, Madison, Wisconsin 53706 1532, USA
    Genetics 155:1667-82. 2000
    ....
  81. ncbi A microbead-based system for identifying and characterizing RNA-protein interactions by flow cytometry
    Alexander S Brodsky
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School and the Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Mol Cell Proteomics 1:922-9. 2002
    ..We propose that this strategy, in combination with emerging coded bead systems, can identify RNAs and RNA sequences important for interacting with RNA-binding proteins on genomic scales...
  82. ncbi Functional redundancy of worm spliceosomal proteins U1A and U2B''
    Tassa Saldi
    Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, CO 80045, USA
    Proc Natl Acad Sci U S A 104:9753-7. 2007
    ..Redundancy results from the fact that one protein can substitute for the other, even though it normally does not...
  83. ncbi p54nrb is a component of the snRNP-free U1A (SF-A) complex that promotes pre-mRNA cleavage during polyadenylation
    Songchun Liang
    Department of Biochemistry and Molecular Biology, UMDNJ-New Jersey Medical School MSB E671, 185 S. Orange Avenue, Newark, NJ 07103, USA
    RNA 12:111-21. 2006
    ..We have explored the function of this complex in RNA processing, specifically cleavage and polyadenylation, by performing immunodepletions followed by reconstitution experiments, and have found that p54(nrb) is critical...
  84. ncbi The role of positively charged amino acids and electrostatic interactions in the complex of U1A protein and U1 hairpin II RNA
    Michael J Law
    Department of Biochemistry and Molecular Biology, University of Southern California, Los Angeles, CA 90089-9176, USA
    Nucleic Acids Res 34:275-85. 2006
    ..Thus, well-positioned positively charged residues can be important for both initial complex formation and complex maintenance, illustrating the multiple roles of electrostatic interactions in protein-RNA complexes...
  85. ncbi The domain of the Bacillus subtilis DEAD-box helicase YxiN that is responsible for specific binding of 23S rRNA has an RNA recognition motif fold
    Shuying Wang
    Department of Structural Biology, Stanford University School of Medicine, California 94305, USA
    RNA 12:959-67. 2006
    ....
  86. ncbi Interactions of Arabidopsis RS domain containing cyclophilins with SR proteins and U1 and U11 small nuclear ribonucleoprotein-specific proteins suggest their involvement in pre-mRNA Splicing
    Zdravko J Lorkovic
    Max F Perutz Laboratories, University Departments at the Vienna Biocenter, Department of Biochemistry, Medical University of Vienna, Dr Bohrgasse 9 3, A 1030 Vienna, Austria
    J Biol Chem 279:33890-8. 2004
    ..Alternatively, binding of CypRS64 to proteins important for 5' splice site recognition suggests its involvement in the dynamics of spliceosome assembly...
  87. ncbi Anti-U1A monoclonal antibodies recognize unique epitope targets of U1A which are involved in the binding of U1 RNA
    Carol S Lutz
    Department of Biochemistry and Molecular Biology, UMDNJ New Jersey Medical School, Newark, NJ 07103, USA
    J Mol Recognit 15:163-70. 2002
    ..These findings suggest that the structure of the U1A protein may be different when not part of the U1snRNP...
  88. ncbi A downstream splicing enhancer is essential for in vitro pre-mRNA splicing
    B G Yue
    Dept of Medical Biochemistry and Microbiology, Uppsala University, Sweden
    FEBS Lett 451:10-4. 1999
    ..Also, splice site pairing occurs efficiently in the absence of exonic SR enhancers, emphasizing the significance of a downstream 5' splice site as the enhancer element in vertebrate splicing...
  89. ncbi The snRNP-free U1A (SF-A) complex(es): identification of the largest subunit as PSF, the polypyrimidine-tract binding protein-associated splicing factor
    C S Lutz
    Department of Microbiology, University of Pennsylvania, School of Medicine, Philadelphia 19104, USA
    RNA 4:1493-9. 1998
    ..This suggests that SF-A and/or the SF-A complex(es) perform an important function in both processing reactions and possibly in last exon definition...
  90. ncbi U snRNP assembly in yeast involves the La protein
    D Xue
    Departments of Cell Biology and Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, 295 Congress Avenue, New Haven, CT 06536, USA
    EMBO J 19:1650-60. 2000
    ..These results suggest that, by stabilizing a 3'-extended form of U4 RNA, Lhp1p facilitates efficient Sm protein binding, thus assisting formation of the U4/U6 snRNP...
  91. ncbi An ATP-dependent, Ran-independent mechanism for nuclear import of the U1A and U2B" spliceosome proteins
    M Hetzer
    European Molecular Biology Laboratory EMBL, D 69117 Heidelberg, Germany
    J Cell Biol 148:293-303. 2000
    ..This activity can be solubilized in the presence of elevated MgCl(2). These data suggest that U1A and U2B" import into the nucleus occurs by a hitherto uncharacterized mechanism...
  92. ncbi Nuclear eukaryotic initiation factor 4E (eIF4E) colocalizes with splicing factors in speckles
    J Dostie
    Department of Biochemistry, McGill University, Montreal, Quebec H3G 1Y6, Canada
    J Cell Biol 148:239-47. 2000
    ..In addition, overexpression of the dual specificity kinase, Clk/Sty, but not of the catalytically inactive form, results in the dispersion of eIF4E nuclear speckles...
  93. ncbi RNA binding specificity of a Drosophila snRNP protein that shares sequence homology with mammalian U1-A and U2-B" proteins
    D S Harper
    Department of Microbiology and Immunology, Duke University Medical Center, Durham, NC 27710
    Nucleic Acids Res 20:3645-50. 1992
    ..Furthermore, D25 bound U1 RNA when transfected into mammalian cells. Thus, D25 appears to be a Drosophila homolog of the mammalian U1-A protein, despite its sequence similarity to U2-B"...
  94. ncbi Luc7p, a novel yeast U1 snRNP protein with a role in 5' splice site recognition
    P Fortes
    European Molecular Biology Laboratory, D 69117 Heidelberg, Germany
    Genes Dev 13:2425-38. 1999
    ..These data suggest that the loss of Luc7p disrupts U1 snRNP-CBC interaction, and that this interaction contributes to normal 5' splice site recognition...
  95. ncbi The SRm160/300 splicing coactivator is required for exon-enhancer function
    A G Eldridge
    Banting and Best Department of Medical Research, University of Toronto, Toronto, ON M5G 1L6, Canada
    Proc Natl Acad Sci U S A 96:6125-30. 1999
    ..The results suggest a model for ESE function in which the SRm160/300 splicing coactivator promotes critical interactions between ESE-bound "activators" and the snRNP machinery of the spliceosome...
  96. ncbi A comprehensive biochemical and genetic analysis of the yeast U1 snRNP reveals five novel proteins
    A Gottschalk
    Institut fur Molekularbiologie und Tumorforschung, Philipps Universitat Marburg, Germany
    RNA 4:374-93. 1998
    ..Finally, we show that Nam8p/Mud15p contributes to the stability of U1 snRNP...
  97. ncbi The yeast splicing factor Mud13p is a commitment complex component and corresponds to CBP20, the small subunit of the nuclear cap-binding complex
    H V Colot
    Howard Hughes Medical Institute, Brandeis University, Department of Biology, Waltham, Massachusetts 02254, USA
    Genes Dev 10:1699-708. 1996
    ..Taken together with the accompanying results for a mammalian system, our data indicate that cap-binding proteins as well as the pre-mRNA cap contribute to early steps in spliceosome assembly...
  98. ncbi Pre-mRNA processing factors are required for nuclear export
    A S Brodsky
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School and the Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    RNA 6:1737-49. 2000
    ..Taken together, these data graphically display the connections between mRNA processing and nuclear export...
  99. ncbi Molecular dynamics simulation of the human U2B" protein complex with U2 snRNA hairpin IV in aqueous solution
    J X Guo
    Eppley Institute, University of Nebraska Medical Center, Omaha, Nebraska, 68198-6805 USA
    Biophys J 81:630-42. 2001
    ..The stabilizing interaction energy due to bridging water molecules was obtained from ab initio Hartree-Fock and density functional theory calculations...
  100. ncbi Modulation of msl-2 5' splice site recognition by Sex-lethal
    P Förch
    Gene Expression Programme, European Molecular Biology Laboratory, Heidelberg, Germany
    RNA 7:1185-91. 2001
    ..Taken together with previous data, we conclude that efficient retention of msl-2 intron involves inhibition of early recognition of both splice sites by SXL...
  101. ncbi NMR studies of U1 snRNA recognition by the N-terminal RNP domain of the human U1A protein
    P W Howe
    MRC Laboratory of Molecular Biology, Cambridge, UK
    EMBO J 13:3873-81. 1994
    ..The quality of the spectra for this complex of 22 kDa demonstrates the feasibility of NMR investigation of RNA-protein complexes...

Research Grants51

  1. Immune Response to Small Nuclear Ribonucleoprotein Autoantigens
    Eric Greidinger; Fiscal Year: 2009
    ..Our research goal is to develop new and effective treatments of these disorders. ..
  2. APOPTOSIS-MODIFIED SELF ANTIGEN IN RHEUMATIC DISEASE
    Eric Greidinger; Fiscal Year: 2002
    ..In the future, this work may lead to the development of specific immunotherapy strategies for the treatment of Ul-70kDa-associated rheumatic disorders. ..
  3. Molecular Dynamics of Induced Fit in U1A-RNA Complexes
    BETHANY KORMOS; Fiscal Year: 2006
    ..abstract_text> ..
  4. Immune Response to Small Nuclear Ribonucleoprotein Autoantigens
    Eric L Greidinger; Fiscal Year: 2010
    ..Our research goal is to develop new and effective treatments of these disorders. ..
  5. Regulation of mRNA Splicing by Mer1p
    Marc Spingola; Fiscal Year: 2007
    ..This project will elucidate how Mer1p and similar proteins function and will offer important insights towards the basis of diseases like Fragile X Syndrome in humans. ..
  6. Mechanisms of MeCP2 gene expression regulation
    CAROL LUTZ; Fiscal Year: 2007
    ..These studies represent an under-explored area of research on MeCP2 gene expression that may have a critical influence in the tissue-specific effects observed in Rett syndrome. ..
  7. 3' end formation of human type I and II collagen mRNAs
    CAROL LUTZ; Fiscal Year: 2007
    ....
  8. Role of G protein Coupling in Fe(ll)-Uptake in Bacteria
    Vinzenz Unger; Fiscal Year: 2006
    ..The fourth aim is to generate crystals of FeoB embedded in a lipid bilayer. Ultimately, this will allow visualization of FeoB, and reveal whether its structure is related to other G protein-coupled membrane proteins. ..
  9. Spatial changes in gene organization during adipogenesis
    ANTHONY IMBALZANO; Fiscal Year: 2007
    ..Such molecules may represent an entirely new class of targets for future therapies. ..
  10. Epigenetic Control of Adipogenesis
    SAID SIF; Fiscal Year: 2010
    ..abstract_text> ..
  11. TRANSCRIPTION FACTOR FUNCTION IN CHROMATIN
    ANTHONY IMBALZANO; Fiscal Year: 2009
    ....
  12. Structure and Function of Copper Transporters
    Vinzenz Unger; Fiscal Year: 2009
    ..Using the substituted cysteine accessibility method and alanine scanning mutagenesis, we will test this hypothesis by determining how the different transmembrane helices contribute to such a copper uptake. ..
  13. TRANSCRIPTION FACTOR FUNCTION IN CHROMATIN
    Anthony N Imbalzano; Fiscal Year: 2010
    ....
  14. TRANSCRIPTION FACTOR FUNCTION IN CHROMATIN
    ANTHONY IMBALZANO; Fiscal Year: 2001
    ....
  15. TRANSCRIPTION FACTOR FUNCTION IN CHROMATIN
    ANTHONY IMBALZANO; Fiscal Year: 2005
    ..Aim 3 will probe the generality of the requirement for SWI/SNF enzymes in different differentiation pathways and embryogenesis. ..
  16. Role of arginine deficiency in pathogenesis of lupus
    Minoru Satoh; Fiscal Year: 2004
    ..abstract_text> ..
  17. Tools for Membrane Protein Structure Determination
    Vinzenz Unger; Fiscal Year: 2004
    ..This approach may make a large number of membrane proteins amenable to 2D-crystallization and structure determination. ..
  18. Mechanisms of post-transcriptional regulation of MeCP2
    CAROL LUTZ; Fiscal Year: 2003
    ..These studies represent under-explored areas of MeCP2 expression that may have a critical influence in the tissue-specific effects observed in Rett syndrome. ..
  19. PROTEIN ARGININE METHYLATION AND RNA MATURATION
    Michael Henry; Fiscal Year: 2003
    ..A better understanding of arginine methylation should not only enhance the understanding of cellular RNA maturation, but also provide insights into viral mechanisms for RNA maturation during cell infection. ..