periplasmic binding proteins

Summary

Summary: Periplasmic proteins that scavenge or sense diverse nutrients. In the bacterial environment they usually couple to transporters or chemotaxis receptors on the inner bacterial membrane.

Top Publications

  1. ncbi Periplasmic binding proteins: a versatile superfamily for protein engineering
    Mary A Dwyer
    Department of Biochemistry, Box 3711, Duke University Medical Center, Durham, North Carolina 27710, USA
    Curr Opin Struct Biol 14:495-504. 2004
  2. pmc Construction and optimization of a family of genetically encoded metabolite sensors by semirational protein engineering
    Karen Deuschle
    Carnegie Institution, 260 Panama Street, Stanford, CA 94305, USA
    Protein Sci 14:2304-14. 2005
  3. ncbi Treponema pallidum TroA is a periplasmic zinc-binding protein with a helical backbone
    Y H Lee
    Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235 8884, USA
    Nat Struct Biol 6:628-33. 1999
  4. pmc The iron-responsive Fur regulon in Yersinia pestis
    He Gao
    College of Life Sciences, Northeast Normal University, Changchun 130024, People s Republic of China
    J Bacteriol 190:3063-75. 2008
  5. ncbi 1.7 A X-ray structure of the periplasmic ribose receptor from Escherichia coli
    S L Mowbray
    Department of Molecular Biology, Swedish University of Agricultural Sciences, Uppsala Biomedical Center
    J Mol Biol 225:155-75. 1992
  6. doi The maltose ATP-binding cassette transporter in the 21st century--towards a structural dynamic perspective on its mode of action
    Enrica Bordignon
    ETH Zurich, Laboratory of Physical Chemistry, Wolfgang Pauli Str 10 CH 8093 Zurich, Switzerland
    Mol Microbiol 77:1354-66. 2010
  7. ncbi Dependence of maltose transport and chemotaxis on the amount of maltose-binding protein
    M D Manson
    J Biol Chem 260:9727-33. 1985
  8. pmc Unliganded maltose-binding protein triggers lactose transport in an Escherichia coli mutant with an alteration in the maltose transport system
    G Merino
    Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    J Bacteriol 179:7687-94. 1997
  9. ncbi Asparagine 394 in putative helix 11 of the galactose-H+ symport protein (GalP) from Escherichia coli is associated with the internal binding site for cytochalasin B and sugar
    T P McDonald
    Department of Biochemistry and Molecular Biology, University of Leeds, Leeds, LS2 9JT, United Kingdom
    J Biol Chem 272:15189-99. 1997
  10. pmc The structure of Escherichia coli BtuF and binding to its cognate ATP binding cassette transporter
    Elizabeth L Borths
    Howard Hughes Medical Institute and Division of Chemistry and Chemical Engineering, Mail Code 114 96, California Institute of Technology, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 99:16642-7. 2002

Research Grants

Detail Information

Publications192 found, 100 shown here

  1. ncbi Periplasmic binding proteins: a versatile superfamily for protein engineering
    Mary A Dwyer
    Department of Biochemistry, Box 3711, Duke University Medical Center, Durham, North Carolina 27710, USA
    Curr Opin Struct Biol 14:495-504. 2004
    ..Extensively redesigned periplasmic binding proteins have been re-introduced into bacteria to function in synthetic signal transduction pathways that ..
  2. pmc Construction and optimization of a family of genetically encoded metabolite sensors by semirational protein engineering
    Karen Deuschle
    Carnegie Institution, 260 Panama Street, Stanford, CA 94305, USA
    Protein Sci 14:2304-14. 2005
    A family of genetically-encoded metabolite sensors has been constructed using bacterial periplasmic binding proteins (PBPs) linearly fused to protein fluorophores...
  3. ncbi Treponema pallidum TroA is a periplasmic zinc-binding protein with a helical backbone
    Y H Lee
    Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235 8884, USA
    Nat Struct Biol 6:628-33. 1999
    ..This unique backbone helical conformation was possibly adopted to limit the hinge motion associated with ligand exchange...
  4. pmc The iron-responsive Fur regulon in Yersinia pestis
    He Gao
    College of Life Sciences, Northeast Normal University, Changchun 130024, People s Republic of China
    J Bacteriol 190:3063-75. 2008
    ..The data presented here give us an overview of the iron-responsive Fur regulon in Y. pestis...
  5. ncbi 1.7 A X-ray structure of the periplasmic ribose receptor from Escherichia coli
    S L Mowbray
    Department of Molecular Biology, Swedish University of Agricultural Sciences, Uppsala Biomedical Center
    J Mol Biol 225:155-75. 1992
    ....
  6. doi The maltose ATP-binding cassette transporter in the 21st century--towards a structural dynamic perspective on its mode of action
    Enrica Bordignon
    ETH Zurich, Laboratory of Physical Chemistry, Wolfgang Pauli Str 10 CH 8093 Zurich, Switzerland
    Mol Microbiol 77:1354-66. 2010
    ....
  7. ncbi Dependence of maltose transport and chemotaxis on the amount of maltose-binding protein
    M D Manson
    J Biol Chem 260:9727-33. 1985
    ..A half-maximal chemotactic response occurred at 25% of the malE+ MBP level, suggesting that in vivo the KD for binding of maltose-loaded MBP to Tar is quite high (approximately 250 microM)...
  8. pmc Unliganded maltose-binding protein triggers lactose transport in an Escherichia coli mutant with an alteration in the maltose transport system
    G Merino
    Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    J Bacteriol 179:7687-94. 1997
    ..The requirement for MBP confirms that unliganded MBP interacts with the inner membrane MalFGK2 complex and that MBP plays a crucial role in triggering the transport process...
  9. ncbi Asparagine 394 in putative helix 11 of the galactose-H+ symport protein (GalP) from Escherichia coli is associated with the internal binding site for cytochalasin B and sugar
    T P McDonald
    Department of Biochemistry and Molecular Biology, University of Leeds, Leeds, LS2 9JT, United Kingdom
    J Biol Chem 272:15189-99. 1997
    ..This work powerfully illustrates how comparisons of the aligned amino acid sequences of homologous membrane proteins of unknown structure and characterization of their phenotypes can be used to map substrate and ligand binding sites...
  10. pmc The structure of Escherichia coli BtuF and binding to its cognate ATP binding cassette transporter
    Elizabeth L Borths
    Howard Hughes Medical Institute and Division of Chemistry and Chemical Engineering, Mail Code 114 96, California Institute of Technology, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 99:16642-7. 2002
    ..These glutamate and arginine residues are conserved among binding proteins and ABC transporters mediating iron and B12 uptake, suggesting that they may have a role in docking and the transmission of conformational changes...
  11. pmc Identification of the periplasmic cobalamin-binding protein BtuF of Escherichia coli
    Nathalie Cadieux
    Department of Microbiology, School of Medicine, University of Virginia, Charlottesville, Virginia 22908 0734, USA
    J Bacteriol 184:706-17. 2002
    ..The open reading frame termed yadT in the E. coli genomic sequence is related in sequence to the periplasmic binding proteins for iron-siderophore complexes and was previously implicated in CN-Cbl uptake in Salmonella. The E...
  12. ncbi A molecular switch created by in vitro recombination of nonhomologous genes
    Gurkan Guntas
    Department of Chemical and Biomolecular Engineering, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA
    Chem Biol 11:1483-7. 2004
    ..The modularity of RG13 was demonstrated by increasing maltose affinity while preserving switching activity. RG13 gave rise to a novel cellular phenotype, illustrating the potential of molecular switches to rewire the cellular circuitry...
  13. ncbi X-ray crystallographic structures of the Escherichia coli periplasmic protein FhuD bound to hydroxamate-type siderophores and the antibiotic albomycin
    Teresa E Clarke
    Structural Biology Research Group, Department of Biological Sciences, University of Calgary, 2500 University Dr N W, Calgary, Alberta T2N 1N4, Canada
    J Biol Chem 277:13966-72. 2002
    ....
  14. ncbi Crystal structures of the BtuF periplasmic-binding protein for vitamin B12 suggest a functionally important reduction in protein mobility upon ligand binding
    Nathan K Karpowich
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    J Biol Chem 278:8429-34. 2003
    ..Furthermore, the high mobility of this domain in free BtuF could provide an entropic driving force for the subsequent release of BtuF required to complete the transport cycle...
  15. pmc Trapping the transition state of an ATP-binding cassette transporter: evidence for a concerted mechanism of maltose transport
    J Chen
    Howard Hughes Medical Institute, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 98:1525-30. 2001
    ..These results suggest a general model explaining how ATP hydrolysis is coupled to substrate transport in which a binding protein stimulates the ATPase activity of its cognate transporter by stabilizing the transition state...
  16. pmc Molecular cloning and characterization of genes required for ribose transport and utilization in Escherichia coli K-12
    A Iida
    J Bacteriol 158:674-82. 1984
    ..Mutants defective in this transport system, but normal for ribokinase , are able to grow normally on high concentrations of the sugar, indicating that there is at least a second, low-affinity transport system for ribose in E. coli K-12...
  17. ncbi The bacterial periplasmic histidine-binding protein. structure/function analysis of the ligand-binding site and comparison with related proteins
    B H Oh
    Department of Chemistry, University of California, Berkeley 94720
    J Biol Chem 269:4135-43. 1994
    Bacterial periplasmic binding proteins are initial receptors in the process of active transport across cell membranes and/or chemotaxis...
  18. pmc The crystal structure of Zn(II)-free Treponema pallidum TroA, a periplasmic metal-binding protein, reveals a closed conformation
    Yong Hwan Lee
    Department of Biochemistry, The University of Missouri Columbia, Columbia, Missouri 65211, USA
    J Bacteriol 184:2300-4. 2002
    ..Ligand binding and release by TroA, and presumably by other members of the MBP cluster, differs from the "Venus flytrap" mechanism utilized by bacterial nonmetal solute-binding receptors...
  19. ncbi DING proteins; novel members of a prokaryotic phosphate-binding protein superfamily which extends into the eukaryotic kingdom
    Anne Berna
    Institut de Biologie Moléculaire des Plantes du C N R S, Universite Louis Pasteur, Institut de Botanique, 28 rue Goethe, 67083 Strasbourg Cedex, France
    Int J Biochem Cell Biol 40:170-5. 2008
    ..Though it has been claimed that all such proteins may originate from pseudomonads, many eukaryotic DING proteins have unique features which are incompatible with a bacterial origin...
  20. ncbi Holo- and apo-bound structures of bacterial periplasmic heme-binding proteins
    Winny W Ho
    Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697 3900, USA
    J Biol Chem 282:35796-802. 2007
    ..Both share a common architecture typical of Class III periplasmic binding proteins. The heme binds in a narrow cleft between the N- and C-terminal binding domains and is coordinated by a ..
  21. pmc Binding and signaling of surface-immobilized reagentless fluorescent biosensors derived from periplasmic binding proteins
    Robert M de Lorimier
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    Protein Sci 15:1936-44. 2006
    ..binding, kinetics, and signal transduction of reagentless fluorescent biosensors based on engineered periplasmic binding proteins. We used thermostable ribose and glucose binding proteins cloned from Thermoanaerobacter tengcongensis ..
  22. ncbi In vitro functional characterization of BtuCD-F, the Escherichia coli ABC transporter for vitamin B12 uptake
    Elizabeth L Borths
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA
    Biochemistry 44:16301-9. 2005
    ....
  23. pmc Structural basis for iron binding and release by a novel class of periplasmic iron-binding proteins found in gram-negative pathogens
    Stephen R Shouldice
    Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, AB, Canada
    J Bacteriol 186:3903-10. 2004
    ..Our results clearly implicate the synergistic anion as the primary mediator of global protein conformation and provide detailed insights into the molecular mechanisms of iron binding and release in the periplasm...
  24. ncbi The Treponema pallidum tro operon encodes a multiple metal transporter, a zinc-dependent transcriptional repressor, and a semi-autonomously expressed phosphoglycerate mutase
    Karsten R O Hazlett
    Center for Microbial Pathogenesis, University of Connecticut Health Center, Farmington, Connecticut 06030 3710, USA
    J Biol Chem 278:20687-94. 2003
    ..Our data also indicate that Gpm expression and, therefore, glycolysis would not be abrogated when T. pallidum encounters high Zn2+ levels...
  25. pmc Ferrichrome transport in Escherichia coli K-12: altered substrate specificity of mutated periplasmic FhuD and interaction of FhuD with the integral membrane protein FhuB
    M R Rohrbach
    Universitat Tubingen, Germany
    J Bacteriol 177:7186-93. 1995
    ..His-tag-FhuD and His-tag-FhuD loaded with aerobactin specifically prevented degradation of FhuB and were cross-linked to FhuB. FhuD loaded with substrate and also FhuD free of substrate were able to interact with FhuB...
  26. pmc Ligand-free open-closed transitions of periplasmic binding proteins: the case of glutamine-binding protein
    Guillermo A Bermejo
    Laboratory of Molecular Biophysics, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biochemistry 49:1893-902. 2010
    ..ability to undergo large-scale domain rearrangements is essential for the substrate-binding function of periplasmic binding proteins (PBPs), which are indispensable for nutrient uptake in Gram-negative bacteria...
  27. pmc The Escherichia coli btuE gene, encodes a glutathione peroxidase that is induced under oxidative stress conditions
    Felipe A Arenas
    Departamento de Biologia, Facultad de Quimica y Biologia, Universidad de Santiago de Chile, Santiago, Chile
    Biochem Biophys Res Commun 398:690-4. 2010
    ..coli response to reactive oxygen species. To our knowledge, this is the first report describing a glutathione peroxidase in E. coli...
  28. ncbi Characterization of the periplasmic heme-binding protein shut from the heme uptake system of Shigella dysenteriae
    Suntara Eakanunkul
    Department of Pharmaceutical Sciences, School of Pharmacy, 20 Penn Street, University of Maryland, Baltimore, Maryland 21201, USA
    Biochemistry 44:13179-91. 2005
    ..The results of these experiments confirmed Tyr-94 as the only axial heme ligand and Tyr-228 as making a significant contribution to the stability of heme-loaded ShuT, albeit without directly interacting with the heme iron...
  29. ncbi 2 A resolution structure of DppA, a periplasmic dipeptide transport/chemosensory receptor
    A V Nickitenko
    Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030, USA
    Biochemistry 34:16585-95. 1995
    The family of about 50 periplasmic binding proteins, which exhibit diverse specificity (e.g...
  30. ncbi Maltose transport system of Escherichia coli: an ABC-type transporter
    H Nikaido
    Department of Molecular and Cell Biology, University of California, Berkeley 94720
    FEBS Lett 346:55-8. 1994
    ..In addition, MBP performs a special function in the translocation of the larger ligands, maltodextrins, perhaps by aligning them for entry into the channel...
  31. pmc Mechanism of maltose transport in Escherichia coli: transmembrane signaling by periplasmic binding proteins
    A L Davidson
    Department of Molecular and Cell Biology, University of California, Berkeley 94720
    Proc Natl Acad Sci U S A 89:2360-4. 1992
    ....
  32. ncbi Crystallographic and biochemical analyses of the metal-free Haemophilus influenzae Fe3+-binding protein
    C M Bruns
    Department of Molecular Biology MB8, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Biochemistry 40:15631-7. 2001
    ..4 x 10(18) M(-1)). This high-affinity ligand binding process is unique among the family of bacterial periplasmic binding proteins and has interesting implications in the mechanism of iron removal from the Fe(3+)-binding proteins ..
  33. ncbi Multiple open forms of ribose-binding protein trace the path of its conformational change
    A J Björkman
    Department of Molecular Biology, Swedish Agricultural University, Uppsala, Sweden
    J Mol Biol 279:651-64. 1998
    ..It seems certain that the conformational path that links the forms described here is that followed during ligand retrieval, and in ligand release into the membrane-bound permease system...
  34. pmc Genetic organization of the yersiniabactin biosynthetic region and construction of avirulent mutants in Yersinia pestis
    S W Bearden
    Department of Microbiology and Immunology, University of Kentucky, Lexington 40536 0084, USA
    Infect Immun 65:1659-68. 1997
    ..Finally, Y. pestis strains with mutations in either the psn or irp2 gene were avirulent in mice when inoculated subcutaneously...
  35. pmc Inactivation of the pst system reduces the virulence of an avian pathogenic Escherichia coli O78 strain
    Martin G Lamarche
    Groupe de Recherche sur les Maladies Infectieuses du Porc GREMIP, Universite de Montreal, Faculte de Medecine Veterinaire, C P 5000, Saint Hyacinthe, Quebec, Canada J2S 7C6
    Infect Immun 73:4138-45. 2005
    ..coli strains from different hosts...
  36. ncbi X-ray structures of the leucine-binding protein illustrate conformational changes and the basis of ligand specificity
    Ulrika Magnusson
    Department of Cell and Molecular Biology, Uppsala University, Biomedical Center, Box 596, S 751 24 Uppsala, Sweden
    J Biol Chem 279:8747-52. 2004
    ..Comparison with earlier structures provides further information about solution conformations, as well as the different specificity of the closely related leucine/isoleucine/valine-binding protein...
  37. pmc Green fluorescent protein functions as a reporter for protein localization in Escherichia coli
    B J Feilmeier
    Department of Microbiology, Iowa State University, Ames 50011, USA
    J Bacteriol 182:4068-76. 2000
    ..These results suggest that GFP could serve as a useful reporter for genetic analysis of bacterial protein export and of protein folding...
  38. ncbi Computational design of receptor and sensor proteins with novel functions
    Loren L Looger
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    Nature 423:185-90. 2003
    ..The biological and biosensing activities of the designed receptors illustrate potential applications of computational design...
  39. ncbi The crystal structure of a thermophilic glucose binding protein reveals adaptations that interconvert mono and di-saccharide binding sites
    Matthew J Cuneo
    The Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA
    J Mol Biol 362:259-70. 2006
    b>Periplasmic binding proteins (PBPs) comprise a protein superfamily that is involved in prokaryotic solute transport and chemotaxis. These proteins have been used to engineer reagentless biosensors to detect natural or non-natural ligands...
  40. pmc The role of the synergistic phosphate anion in iron transport by the periplasmic iron-binding protein from Haemophilus influenzae
    Ali G Khan
    Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, AB, Canada T2N 4N1
    Biochem J 403:43-8. 2007
    ..The results suggest that the transport of iron by FbpA is not dependent on binding of phosphate in the synergistic anion-binding site...
  41. pmc Model of maltose-binding protein/chemoreceptor complex supports intrasubunit signaling mechanism
    Y Zhang
    Department of Biology, Texas A and M University, College Station, TX 77843, USA
    Proc Natl Acad Sci U S A 96:939-44. 1999
    ....
  42. pmc Analysis of ligand binding to a ribose biosensor using site-directed mutagenesis and fluorescence spectroscopy
    Natalie C Vercillo
    Department of Chemistry, University of Richmond, Gottwald Center for Sciences, Richmond, Virginia 23173, USA
    Protein Sci 16:362-8. 2007
    ..The data collected from this study may be incorporated into design algorithms to help create more stable biosensors and optimize signal transduction properties for a variety of important analytes...
  43. ncbi Asymmetry in the structure of the ABC transporter-binding protein complex BtuCD-BtuF
    Rikki N Hvorup
    Institute of Molecular Biology and Biophysics, ETH Zurich, HPK D14 3, 8093 Zurich, Switzerland
    Science 317:1387-90. 2007
    ..A comparison with BtuCD and the homologous HI1470/71 protein suggests that the structure of BtuCD-F may reflect a posttranslocation intermediate...
  44. ncbi Structure of D-allose binding protein from Escherichia coli bound to D-allose at 1.8 A resolution
    B N Chaudhuri
    Department of Molecular Biology, Uppsala University, Uppsala, SE 751 24, Sweden
    J Mol Biol 286:1519-31. 1999
    ..Thus, ALBP can function as a low affinity transporter for D-ribose. The significance of these results is discussed in the context of the function of allose and ribose transport systems...
  45. ncbi Development of a fluorescent nanosensor for ribose
    Ida Lager
    ZMBP Tübingen, Plant Physiology, Tubingen, Germany
    FEBS Lett 553:85-9. 2003
    ..Inhibitor studies suggest that uptake is mediated by a monosaccharide transporter of the GLUT family, however, ribose taken up into the cell was not or only slowly released, indicating irreversibility of uptake...
  46. ncbi Probing protein-protein interactions. The ribose-binding protein in bacterial transport and chemotaxis
    A J Björkman
    Department of Molecular Biology, Swedish University of Agricultural Sciences, Uppsala
    J Biol Chem 269:30206-11. 1994
    ..2-A resolution) showed that the changes in local structure were accompanied by a diffuse pattern of structural changes in the surrounding region, implying that the suppression derives from a combination of sources...
  47. pmc The backbone structure of the thermophilic Thermoanaerobacter tengcongensis ribose binding protein is essentially identical to its mesophilic E. coli homolog
    Matthew J Cuneo
    The Institute for Biological Structure and Design and the Department of Biochemistry, Duke University Medical Center, Durham, North Carolina, 27710, USA
    BMC Struct Biol 8:20. 2008
    ..Of particular interest are pairs of homologous structures that are structurally very similar, but differ significantly in thermal stability...
  48. ncbi The structure of the ferric siderophore binding protein FhuD complexed with gallichrome
    T E Clarke
    Department of Biological Sciences, University of Calgary, 2500 University Drive N W, Calgary, Alberta, Canada T2N 1N4
    Nat Struct Biol 7:287-91. 2000
    ..FhuD possesses a novel fold, suggesting that its mechanisms of ligand binding and release are different from other structurally characterized periplasmic ligand binding proteins...
  49. ncbi The Tsr chemosensory transducer of Escherichia coli assembles into the cytoplasmic membrane via a SecA-dependent process
    J F Gebert
    Department of Biology, University of Konstanz, Federal Republic of Germany
    J Biol Chem 263:16652-60. 1988
    ..Moreover, assembly of the Tsr protein seems to be closely coupled to its synthesis...
  50. pmc Identification and characterization of dppA, an Escherichia coli gene encoding a periplasmic dipeptide transport protein
    E R Olson
    Molecular Biology Research, Upjohn Company, Kalamazoo, Michigan 49007
    J Bacteriol 173:234-44. 1991
    ..Transcription of dppA is repressed by the presence of casamino acids, suggesting that the cell alters its dipeptide transport capabilities in response to an environmental signal...
  51. ncbi Computational design of a biologically active enzyme
    Mary A Dwyer
    Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA
    Science 304:1967-71. 2004
    ..The inherent generality of the design method suggests that many enzymes can be designed by this approach...
  52. ncbi Mutations which alter the function of the signal sequence of the maltose binding protein of Escherichia coli
    H Bedouelle
    Nature 285:78-81. 1980
    ..In most cases, the change of a single hydrophobic or uncharged amino acid to a charged amino acid within the signal sequence is sufficient to block the secretion process...
  53. ncbi Degradation versus aggregation of misfolded maltose-binding protein in the periplasm of Escherichia coli
    J M Betton
    Unité de Programmation Moléculaire et de Toxicologie Génétique CNRS URA1444, Departement des Biotechnologies, Institut Pasteur, 25, rue du Docteur Roux 75015 Paris, France
    J Biol Chem 273:8897-902. 1998
    ..A kinetic competition between folding, aggregation, and degradation is proposed as a general model for the biogenesis of periplasmic proteins...
  54. pmc Domain-swapping analysis of FtsI, FtsL, and FtsQ, bitopic membrane proteins essential for cell division in Escherichia coli
    L M Guzman
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Bacteriol 179:5094-103. 1997
    ....
  55. pmc Transduction of envelope stress in Escherichia coli by the Cpx two-component system
    T L Raivio
    Department of Molecular Biology, Princeton University, New Jersey 08544, USA
    J Bacteriol 179:7724-33. 1997
    ..Further, the sequence and phenotypes of periplasmic cpx* mutations suggest that interactions with a periplasmic signaling molecule may normally dictate a decreased kinase/phosphatase ratio under nonstress conditions...
  56. ncbi prl mutations in the Escherichia coli secG gene
    S Bost
    Departement de Pathologie, Universite de Geneve, CH 1211 Geneva, Switzerland
    J Biol Chem 272:4087-93. 1997
    ..These results support the notion that SecG contributes to signal sequence recognition, and suggest that it may also contribute to the topology of integral membrane proteins...
  57. pmc MinCD-independent inhibition of cell division by a protein that fuses MalE to the topological specificity factor MinE
    S Pichoff
    Laboratoire de Microbiologie et de Génétique Moléculaire du CNRS, Toulouse, France
    J Bacteriol 179:4616-9. 1997
    ..We also report that high-level synthesis of MalE disturbs nucleoid partitioning...
  58. ncbi Maltose-binding protein interacts simultaneously and asymmetrically with both subunits of the Tar chemoreceptor
    P J Gardina
    Department of Biology, Texas A and M University, College Station 77843 3258, USA
    Mol Microbiol 23:1181-91. 1997
    ....
  59. pmc Subunit interactions in ABC transporters: a conserved sequence in hydrophobic membrane proteins of periplasmic permeases defines an important site of interaction with the ATPase subunits
    M Mourez
    Unite de Programmation Moleculaire et Toxicologie Genetique, CNRS URA 1444, Institut Pasteur, Paris, France
    EMBO J 16:3066-77. 1997
    ..They map mainly in the putative helical domain of MalK, suggesting that EAA regions may constitute a recognition site for the ABC ATPase helical domain...
  60. ncbi An Escherichia coli gene (yaeO) suppresses temperature-sensitive mutations in essential genes by modulating Rho-dependent transcription termination
    S Pichoff
    Laboratoire de Microbiologie et de Génétique Moléculaire du CNRS, Toulouse, France
    Mol Microbiol 29:859-69. 1998
    ..Our data indicate that the suppression is not caused by overexpression of the mutated genes, but presumably by indirect stabilization of the mutated proteins...
  61. pmc Functional characterization and regulation of gadX, a gene encoding an AraC/XylS-like transcriptional activator of the Escherichia coli glutamic acid decarboxylase system
    Angela Tramonti
    Dipartimento di Scienze Biochimiche A Rossi Fanelli, Centro di Biologia Molecolare del Consiglio Nazionale delle Ricerche, Universita di Roma La Sapienza, Piazzale Aldo Moro 5, 00185 Rome, Italy
    J Bacteriol 184:2603-13. 2002
    ..Gel shift and DNase I footprinting analyses with a MalE-GadX fusion protein demonstrate that GadX binds gadA and gadBC promoters at different sites and with different binding affinities...
  62. pmc Molecular cloning and characterization of the Salmonella enterica Serovar Paratyphi B rma Gene, which confers multiple drug resistance in Escherichia coli
    Mahmoud A Yassien
    Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt
    Antimicrob Agents Chemother 46:360-6. 2002
    ..Furthermore, overexpression of rma in E. coli caused changes in the outer membrane protein profile that were similar to those reported for MarA. These results suggest that Rma might act as a transcriptional activator of the marA regulon...
  63. ncbi The role of FhuD2 in iron(III)-hydroxamate transport in Staphylococcus aureus. Demonstration that FhuD2 binds iron(III)-hydroxamates but with minimal conformational change and implication of mutations on transport
    M Tom Sebulsky
    Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada
    J Biol Chem 278:49890-900. 2003
    ....
  64. ncbi Genomic arrangement of a putative operon involved in maltose transport in the Mycobacterium tuberculosis complex and Mycobacterium leprae
    S M Borich
    Mycobacteriology Research Laboratory, Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North, New Zealand
    Microbios 102:7-15. 2000
    ..leprae...
  65. ncbi ATP modulates subunit-subunit interactions in an ATP-binding cassette transporter (MalFGK2) determined by site-directed chemical cross-linking
    S Hunke
    Humboldt Universitat zu Berlin, Institut für Biologie Bakterienphysiologie, Chausseestrasse 117, D 10115 Berlin, Germany
    J Biol Chem 275:15526-34. 2000
    ..These interactions are strongly modulated by MgATP, indicating a structural rearrangement of the subunits during the transport cycle. These data are discussed with respect to current transport models...
  66. pmc Cell division in Escherichia coli: role of FtsL domains in septal localization, function, and oligomerization
    J M Ghigo
    Unité des Membranes Bactériennes, Institut Pasteur CNRS URA 1300, 75724 Paris Cedex 15, France
    J Bacteriol 182:116-29. 2000
    ..This coiled-coil motif is conserved in all gram-negative and gram-positive FtsL homologues identified so far. Our data suggest that most of the FtsL molecule is a helical coiled coil involved in FtsL multimerization...
  67. pmc Characterization of heme uptake cluster genes in the fish pathogen Vibrio anguillarum
    Susana Mouriño
    Department of Microbiology and Parasitology, Institute of Aquaculture and Faculty of Biology, University of Santiago de Compostela, Santiago de Compostela 15782, Spain
    J Bacteriol 186:6159-67. 2004
    ..The V. anguillarum heme uptake cluster shows some differences in gene arrangement when compared to homologous clusters described for other Vibrio species...
  68. ncbi Recruitment of ZipA to the division site by interaction with FtsZ
    Z Liu
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City 66160, USA
    Mol Microbiol 31:1853-61. 1999
    ..These results are consistent with ZipA acting after Z ring formation, possibly to link the membrane to FtsZ filaments during invagination of the septum...
  69. ncbi Transmembrane topology of the two FhuB domains representing the hydrophobic components of bacterial ABC transporters involved in the uptake of siderophores, haem and vitamin B12
    W Groeger
    Universitat Tubingen, Germany
    Microbiology 144:2759-69. 1998
    ..However, in the 'siderophore family' proteins this putative ATPase interaction loop is followed by four instead of two transmembrane spans...
  70. ncbi Structural biology. Not just another ABC transporter
    Amy L Davidson
    Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX 77030, USA
    Science 296:1038-40. 2002
  71. ncbi Maltose chemotaxis involves residues in the N-terminal and C-terminal domains on the same face of maltose-binding protein
    Y Zhang
    Department of Biology, Texas A and M University, College Station 77843 3258
    J Biol Chem 267:22813-20. 1992
    ....
  72. ncbi Structure of the L-leucine-binding protein refined at 2.4 A resolution and comparison with the Leu/Ile/Val-binding protein structure
    J S Sack
    Department of Biochemistry, Baylor College of Medicine, Houston, TX 77030
    J Mol Biol 206:193-207. 1989
    ..These substitutions, by themselves, fail to clearly explain the differences in the specificities for branched aliphatic amino acids...
  73. ncbi E. coli mutant pleiotropically defective in the export of secreted proteins
    D B Oliver
    Cell 25:765-72. 1981
    ..The principle behind the genetic selection employed here should be useful in obtaining other secretion mutants to characterize the cell's secretion machinery...
  74. pmc A new genetic selection identifies essential residues in SecG, a component of the Escherichia coli protein export machinery
    S Bost
    Department de Pathologie, Universite de Geneve, Switzerland
    EMBO J 14:4412-21. 1995
    ..Most suppressors map to secY, and several are allele-specific. Finally, SecG overexpression accelerates the kinetics of protein export, suggesting that there are two types of functional translocation complexes: with or without SecG...
  75. pmc Membrane insertion of the bacterial signal transduction protein ToxR and requirements of transcription activation studied by modular replacement of different protein substructures
    H Kolmar
    Institut für Molekulare Genetik, George August Universität Göttingen, Germany
    EMBO J 14:3895-904. 1995
    ..Possible applications of ToxR as a technical tool for analysing protein-protein interactions between pairs of arbitrary TM domains are discussed...
  76. pmc The topology of the brown adipose tissue mitochondrial uncoupling protein determined with antibodies against its antigenic sites revealed by a library of fusion proteins
    B Miroux
    Centre de Recherches en Endocrinologie Moléculaire et Développement CEREMOD, CNRS, Meudon, France
    EMBO J 12:3739-45. 1993
    ..This topological study together with previous data on the UCP provides an experimental basis for the predicted structure of the UCP and for other homologous carrier proteins...
  77. ncbi Purification of a MalE-SoxS fusion protein and identification of the control sites of Escherichia coli superoxide-inducible genes
    W P Fawcett
    Department of Biological Sciences, University of Maryland, Catonsville 21228
    Mol Microbiol 14:669-79. 1994
    ..Moreover, MalE-SoxS protected from DNase I attack 22-27 bp segments immediately adjacent to or overlapping the -35 hexamers of the zwf, sodA, nfo, micF, and fumC promoters. The protected regions revealed a consensus 'soxbox' sequence...
  78. ncbi Transport of haemin across the cytoplasmic membrane through a haemin-specific periplasmic binding-protein-dependent transport system in Yersinia enterocolitica
    I Stojiljkovic
    Lehrstuhl für Mikrobiologie II, Tubingen, Germany
    Mol Microbiol 13:719-32. 1994
    ..We propose a model of haemin utilization in Y. enterocolitica in which HemT, HemU and HemV proteins transport haemin into the cytoplasm where it is degraded by HemS thereby liberating the iron...
  79. pmc In vivo studies of the role of SecA during protein export in Escherichia coli
    S Y Chun
    Department of Biochemistry and Biophysics, Washington State University, Pullman 99164 4660
    J Bacteriol 176:4197-203. 1994
    ..The results indicate that the mutational change and treatment with sodium azide inhibit export by affecting different steps in the cycle of ATP binding and hydrolysis by SecA...
  80. ncbi Identical mutations at corresponding positions in two homologous proteins with nonidentical effects
    A J Björkman
    Department of Molecular Biology, Swedish University of Agricultural Sciences, Uppsala
    J Biol Chem 269:11196-200. 1994
    ..These results are consistent with the concept that these proteins have slightly different ways of interacting with the membrane components in transport and chemotaxis...
  81. ncbi Prediction of the structural similarity between spermidine/putrescine-binding protein and maltose-binding protein
    Y Matsuo
    Protein Engineering Research Institute, Osaka, Japan
    FEBS Lett 345:23-6. 1994
    ..The conservation of this motif among certain bacterial periplasmic binding proteins suggests a common functional role for this region as well as an evolutionary relationship between them.
  82. pmc Reconstitution of maltose chemotaxis in Escherichia coli by addition of maltose-binding protein to calcium-treated cells of maltose regulon mutants
    J M Brass
    J Bacteriol 157:881-90. 1984
    ....
  83. ncbi The nucleotide sequence of the gene for malF protein, an inner membrane component of the maltose transport system of Escherichia coli. Repeated DNA sequences are found in the malE-malF intercistronic region
    S Froshauer
    J Biol Chem 259:10896-903. 1984
    ..Further analysis of this region may help in understanding the observed step-down in synthesis of the MalF protein...
  84. pmc Identification of five new essential genes involved in the synthesis of a secreted protein in Escherichia coli
    D B Oliver
    J Bacteriol 161:285-91. 1985
    ..Since mutants in any of seven genes as well as secA amber mutants halt or reduce the synthesis of an exported protein, it appears that E. coli may possess a general and complex mechanism for coupling protein synthesis and secretion...
  85. pmc Aspartate and maltose-binding protein interact with adjacent sites in the Tar chemotactic signal transducer of Escherichia coli
    P Gardina
    Department of Biology, Texas A and M University, College Station 77843 3258
    J Bacteriol 174:1528-36. 1992
    ..We conclude that aspartate and maltose-binding protein interact with adjacent and partially overlapping regions in the periplasmic domain of Tar to initiate attractant signalling...
  86. ncbi Completion of the nucleotide sequence of the 'maltose B' region in Salmonella typhimurium: the high conservation of the malM gene suggests a selected physiological role for its product
    E Schneider
    Universitat Osnabruck, Fachbereich Biologie chemie, Abteilung Mikrobiologie, Germany
    Biochim Biophys Acta 1129:223-7. 1992
    ..The malM gene, encoding a periplasmic protein of unknown function in Escherichia coli, is a highly conserved as genes encoding proteins of known function from the same region...
  87. pmc Decoding signals for membrane protein assembly using alkaline phosphatase fusions
    K McGovern
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115
    EMBO J 10:2773-82. 1991
    ..The strength of cytoplasmic domains as topogenic signals varies, correlated with the density of positively charged amino acids within them...
  88. ncbi Membrane insertion of the Escherichia coli MalF protein in cells with impaired secretion machinery
    K McGovern
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115
    J Biol Chem 266:20870-6. 1991
    ..Our results suggest that MalF can assemble in the membrane independently of the bacterial secretion machinery...
  89. ncbi The 2.3-A resolution structure of the maltose- or maltodextrin-binding protein, a primary receptor of bacterial active transport and chemotaxis
    J C Spurlino
    Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030
    J Biol Chem 266:5202-19. 1991
    ..abstract truncated at 250 words)..
  90. pmc Genetic analysis of membrane protein topology by a sandwich gene fusion approach
    M Ehrmann
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115
    Proc Natl Acad Sci U S A 87:7574-8. 1990
    ..Thus, the sandwich fusion approach can give a more accurate picture of membrane protein topology...
  91. pmc Novel secA alleles improve export of maltose-binding protein synthesized with a defective signal peptide
    J D Fikes
    Department of Microbiology and Immunology, School of Medicine, University of North Carolina, Chapel Hill 27514
    J Bacteriol 171:402-9. 1989
    ..The properties of these suppressor and insertion mutations provide some insight into the role of SecA in the protein export process...
  92. pmc Maltose chemoreceptor of Escherichia coli: interaction of maltose-binding protein and the tar signal transducer
    M Kossmann
    Department of Biology, Texas A and M University, College Station 77843
    J Bacteriol 170:4516-21. 1988
    ..In turn, arginines at residues 69 and 73 of Tar must be involved in the recognition of maltose-bound MBP and/or in the production of the attractant signal generated by Tar in response to maltose-bound MBP...
  93. pmc Diverse effects of the MalE-LacZ hybrid protein on Escherichia coli cell physiology
    K Ito
    J Bacteriol 167:201-4. 1986
    ..These results indicate that the hybrid protein somehow generates a signal or stress that is similar to what the cell experiences at elevated temperatures...
  94. pmc Regions of maltose-binding protein that influence SecB-dependent and SecA-dependent export in Escherichia coli
    S M Strobel
    Department of Microbiology and Immunology, School of Medicine, University of North Carolina, Chapel Hill 27599 7290
    J Bacteriol 175:6988-95. 1993
    ..The export of these altered MBP species was also less affected in secA mutant cells and in cells treated with sodium azide. These results present additional evidence for the targeting role of SecB...
  95. ncbi Characterization of the structural requirements for assembly and nucleotide binding of an ATP-binding cassette transporter. The maltose transport system of Escherichia coli
    C H Panagiotidis
    Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, New York 10032
    J Biol Chem 268:23685-96. 1993
    ..Excess MalK competed with the MalK-LacZ hybrid protein for sites in the membrane and resulted in the hybrid fractionating as a soluble protein.(ABSTRACT TRUNCATED AT 400 WORDS)..
  96. pmc prlA suppression of defective export of maltose-binding protein in secB mutants of Escherichia coli
    O Francetic
    Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111
    J Bacteriol 175:4036-44. 1993
    ..Sako and T. Iino, J. Bacteriol. 170:5389-5391, 1988). Comparison of the prlA1024 mutant and the prlA4 double mutant provides a possible explanation for the isolation of these prlA alleles...
  97. ncbi Genetic analysis of periplasmic binding protein dependent transport in Escherichia coli. Each lobe of maltose-binding protein interacts with a different subunit of the MalFGK2 membrane transport complex
    L I Hor
    Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, NY 10032
    J Mol Biol 233:659-70. 1993
    ..The locations of residues 13, 14 and 210 on the three-dimensional structure of MBP are in keeping with this model...
  98. ncbi Molecular cloning of a maltose transport gene from Bacillus stearothermophilus and its expression in Escherichia coli K-12
    E C Liong
    Department of Microbiology, University of Sydney, NSW, Australia
    Mol Gen Genet 243:343-52. 1994
    ..coli mutants. Hence MalA protein was the only protein necessary for maltose transport, but despite giving a detectable but low level of transport function in E. coli, the protein was very poorly expressed and could not be identified...
  99. ncbi Structure of the maltodextrin-uptake locus of Streptococcus pneumoniae. Correlation to the Escherichia coli maltose regulon
    A Puyet
    Centro de Investigaciones Biologicas, C S I C, Madrid, Spain
    J Mol Biol 230:800-11. 1993
    ..The processing of mRNA has not been reported in the Gram-negative maltose regulons, and may suggest either a less evolved or a divergent system for the control of gene expression of this regulon in S. pneumoniae...
  100. ncbi Crystal structure of PotD, the primary receptor of the polyamine transport system in Escherichia coli
    S Sugiyama
    Protein Engineering Research Institute, Suita, Osaka, Japan
    J Biol Chem 271:9519-25. 1996
    ..The overall fold of PotD is similar to that of other periplasmic binding proteins, and in particular to the maltodextrin-binding protein from E...
  101. ncbi Folding of a mutant maltose-binding protein of Escherichia coli which forms inclusion bodies
    J M Betton
    Departement des Biotechnologies, Institut Pasteur, 25, rue du Docteur Roux, 75015 Paris, France
    J Biol Chem 271:8046-52. 1996
    ..5 kcal/mol) and that folding intermediates have a high tendency to form aggregates. In conclusion, the observed phenotype of cells expressing malE31 can be explained by a defective folding pathway of the protein...

Research Grants57

  1. PERIPLASMIC RECEPTORS OF ACTIVE TRANSPORT AND CHEMOTAXIS
    Florante Quiocho; Fiscal Year: 1993
    A molecular understanding of the role of periplasmic binding proteins in active transport and chemotaxis in bacteria requires a detailed pictures of this family of proteins...
  2. IRON UPTAKE AS A VIRULENCE FACTOR IN PATHOGENIC VIBRIOS
    JORGE CROSA; Fiscal Year: 2000
    ..An understanding of signal transduction in bacteria may also reveal new strategies for combating infectious disease. Results obtained in this system are thus directly applicable to other systems involved in pathogenesis. ..
  3. Mulitvalent Ligands as Effectors
    LAURA KIESSLING; Fiscal Year: 2009
    ..Aim 1 is focused on understanding how periplasmic binding proteins (PBPs) function...
  4. Mulitvalent Ligands as Effectors
    LAURA KIESSLING; Fiscal Year: 2007
    ..Aim 1 is focused on understanding how periplasmic binding proteins (PBPs) function...
  5. MOLECULAR PHARMACOLOGY OF GLUTAMATE RECEPTORS
    Keith Williams; Fiscal Year: 2005
    ..domain (R domain) of the NMDA receptor, that may have an overall structure similar to bacterial periplasmic binding proteins. Thus, a second approach will involve biochemical and ligand-binding studies of purified, soluble R ..
  6. MOLECULAR PHARMACOLOGY OF GLUTAMATE RECEPTORS
    Keith Williams; Fiscal Year: 2007
    ..domain (R domain) of the NMDA receptor, that may have an overall structure similar to bacterial periplasmic binding proteins. Thus, a second approach will involve biochemical and ligand-binding studies of purified, soluble R ..
  7. Coordinated control of a regulatory network by gcvB RNA
    GEORGE STAUFFER; Fiscal Year: 2007
    ..g., oppA and dppA, encoding the oligopeptide and dipeptide periplasmic binding proteins, respectively)...
  8. Global Mechanisms of Bacteriocin Resistance in Listeria
    Kurt Miller; Fiscal Year: 2007
    ..The research ultimately will lead to better methods for prevention of food contamination and disease caused by the deadly pathogen, Listeria monocytogenes. ..
  9. Molecular Mechanisms of Neuronal Migrations
    Zygmunt Derewenda; Fiscal Year: 2009
    ..A comparison of results based on the Xenopus assays with the pull-down assays using mouse brain extracts will provide us with a unique database for Nud-protein interactions and invaluable insight into their function. ..
  10. Mechanistic and Structure-Function Studies of Human DNA Polymerase Lambda
    Zucai Suo; Fiscal Year: 2007
    ....
  11. Role of Shigella two component regulation systems in intracellular adaptation
    Laura Runyen Janecky; Fiscal Year: 2007
    ..The research described in this proposal will be useful for designing more effective therapies and will yield information applicable to other similar pathogens. ..
  12. Mechanistic and Structure-Function Studies of Human DNA Polymerase Lambda
    Zucai Suo; Fiscal Year: 2010
    ....
  13. Mechanistic and Structure-Function Studies of Human DNA Polymerase Lambda
    Zucai Suo; Fiscal Year: 2009
    ....
  14. Molecular Mechanisms of Neuronal Migrations
    Zygmunt S Derewenda; Fiscal Year: 2010
    ..A comparison of results based on the Xenopus assays with the pull-down assays using mouse brain extracts will provide us with a unique database for Nud-protein interactions and invaluable insight into their function. ..
  15. EXPORT OF PROTEINS IN ESCHERICHIA COLI
    Linda L Randall; Fiscal Year: 2010
    ..Therefore, what we learn by studying bacterial export will be applicable to the phenomenon in all cells, from bacteria to humans. ..
  16. Mechanistic and Structure-Function Studies of Human DNA Polymerase Lambda
    Zucai Suo; Fiscal Year: 2009
    ....
  17. EXPORT OF PROTEINS IN ESCHERICHIA COLI
    Linda Randall; Fiscal Year: 2007
    ..Therefore, what we learn by studying bacterial export will be applicable to the phenomenon in all cells, from bacteria to humans. ..
  18. MECHANISMS OF LIPID-MEDIATED SIGNAL TRANSDUCTION
    Zygmunt Derewenda; Fiscal Year: 2001
    ....
  19. Molecular Mechanisms of Neuronal Migration
    Zygmunt Derewenda; Fiscal Year: 2005
    ..We will also attempt to crystallize complexes of Lisi and DCX with downstream signaling partners. ..
  20. Rapid protein crystallization by surface mutagenesis
    Zygmunt Derewenda; Fiscal Year: 2004
    ..Funds are requested to further test the general applicability and success rate of this method, and to apply the method to a subset of targets selected for the Structural Genomic Initiative. ..
  21. Preparing high resolution membrane protein crystals
    Zygmunt Derewenda; Fiscal Year: 2005
    ..abstract_text> ..
  22. EXPORT OF PROTEINS IN ESCHERICHIA COLI
    Linda Randall; Fiscal Year: 2006
    ..Conclusions that are based on work in vitro with purified proteins will be confirmed in vivo. It is from integration of data obtained through diverse approaches that the investigators will learn the most. ..