nucleocytoplasmic transport proteins

Summary

Summary: Proteins involved in the process of transporting molecules in and out the cell nucleus. Included here are: NUCLEOPORINS, which are membrane proteins that form the NUCLEAR PORE COMPLEX; KARYOPHERINS, which carry molecules through the nuclear pore complex; and proteins that play a direct role in the transport of karyopherin complexes through the nuclear pore complex.

Top Publications

  1. pmc Adaptor Aly and co-adaptor Thoc5 function in the Tap-p15-mediated nuclear export of HSP70 mRNA
    Jun Katahira
    Biomolecular Dynamics Laboratory, Graduate School of Frontier Biosciences, Osaka University, Yamadaoka, Suita, Osaka, Japan Japan
    EMBO J 28:556-67. 2009
  2. pmc ATP is required for interactions between UAP56 and two conserved mRNA export proteins, Aly and CIP29, to assemble the TREX complex
    Kobina Dufu
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 24:2043-53. 2010
  3. pmc Identification of mRNAs that are spliced but not exported to the cytoplasm in the absence of THOC5 in mouse embryo fibroblasts
    Anuja Guria
    Institut fur Biochemie, OE4310, Medizinische Hochschule Hannover, D 30623 Hannover, Germany
    RNA 17:1048-56. 2011
  4. ncbi The mRNA export protein DBP5 binds RNA and the cytoplasmic nucleoporin NUP214 in a mutually exclusive manner
    Holger von Moeller
    Max Planck Institute of Biochemistry, Department of Structural Cell Biology, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Nat Struct Mol Biol 16:247-54. 2009
  5. ncbi TAP, the human homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus
    P Grüter
    Department of Molecular Biology, University of Geneva, Switzerland
    Mol Cell 1:649-59. 1998
  6. pmc The yeast THO complex and mRNA export factors link RNA metabolism with transcription and genome instability
    S Jimeno
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, 41012 Sevilla, Spain
    EMBO J 21:3526-35. 2002
  7. ncbi THO/Sub2p functions to coordinate 3'-end processing with gene-nuclear pore association
    Mathieu Rougemaille
    LEA Laboratory of Nuclear RNA Metabolism, Centre de Genetique Moleculaire, Centre National de la Recherche Scientifique UPR2167, 1, av de la Terrasse, 91190, Gif sur Yvette, France
    Cell 135:308-21. 2008
  8. pmc Imaging and characterizing influenza A virus mRNA transport in living cells
    Wei Wang
    State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, China
    Nucleic Acids Res 36:4913-28. 2008
  9. pmc Ubiquitin-associated domain of Mex67 synchronizes recruitment of the mRNA export machinery with transcription
    Carole Gwizdek
    Institut Jacques Monod, Unite Mixte de Recherche 7592, Centre National de la Recherche Scientifique, Universités Paris VI and VII, 2 place Jussieu, Tour 43, 75251 Paris Cedex 05, France
    Proc Natl Acad Sci U S A 103:16376-81. 2006
  10. pmc Yra1p, a conserved nuclear RNA-binding protein, interacts directly with Mex67p and is required for mRNA export
    K Strasser
    BZH, Biochemie Zentrum Heidelberg, Germany
    EMBO J 19:410-20. 2000

Detail Information

Publications227 found, 100 shown here

  1. pmc Adaptor Aly and co-adaptor Thoc5 function in the Tap-p15-mediated nuclear export of HSP70 mRNA
    Jun Katahira
    Biomolecular Dynamics Laboratory, Graduate School of Frontier Biosciences, Osaka University, Yamadaoka, Suita, Osaka, Japan Japan
    EMBO J 28:556-67. 2009
    ..Thus, through the combinatorial use of an adaptor (e.g., Aly) and co-adapter (e.g., Thoc5), Tap-p15 could function as an export receptor for different classes of mRNAs...
  2. pmc ATP is required for interactions between UAP56 and two conserved mRNA export proteins, Aly and CIP29, to assemble the TREX complex
    Kobina Dufu
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 24:2043-53. 2010
    ..We conclude that the interaction of two conserved export proteins, CIP29 and Aly, with UAP56 is strictly regulated by ATP during assembly of the TREX complex...
  3. pmc Identification of mRNAs that are spliced but not exported to the cytoplasm in the absence of THOC5 in mouse embryo fibroblasts
    Anuja Guria
    Institut fur Biochemie, OE4310, Medizinische Hochschule Hannover, D 30623 Hannover, Germany
    RNA 17:1048-56. 2011
    ..Furthermore, Hsp70 mRNA was exported in the absence of THOC5 at 37°C, but not under heat shock condition (42°C), suggesting that THOC5 may be required for mRNA export under stress and/or upon signaling-induced conditions...
  4. ncbi The mRNA export protein DBP5 binds RNA and the cytoplasmic nucleoporin NUP214 in a mutually exclusive manner
    Holger von Moeller
    Max Planck Institute of Biochemistry, Department of Structural Cell Biology, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Nat Struct Mol Biol 16:247-54. 2009
    ..More generally, they provide a paradigm for how binding of regulators can specifically inhibit DEAD-box proteins...
  5. ncbi TAP, the human homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus
    P Grüter
    Department of Molecular Biology, University of Geneva, Switzerland
    Mol Cell 1:649-59. 1998
    ..Thus, TAP, like its yeast homolog Mex67p, is a bona fide mRNA nuclear export mediator. TAP is the second cellular RNA binding protein shown to be directly involved in the export of its target RNA...
  6. pmc The yeast THO complex and mRNA export factors link RNA metabolism with transcription and genome instability
    S Jimeno
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, 41012 Sevilla, Spain
    EMBO J 21:3526-35. 2002
    ..These results indicate a functional link between the processes of elongation and metabolism of nascent mRNA mediated by THO and mRNA export proteins, which have important consequences for the maintenance of genome stability...
  7. ncbi THO/Sub2p functions to coordinate 3'-end processing with gene-nuclear pore association
    Mathieu Rougemaille
    LEA Laboratory of Nuclear RNA Metabolism, Centre de Genetique Moleculaire, Centre National de la Recherche Scientifique UPR2167, 1, av de la Terrasse, 91190, Gif sur Yvette, France
    Cell 135:308-21. 2008
    ..Our data strongly suggests that the THO complex/Sub2p function is required to coordinate events leading to the acquisition of export competence at a step that follows commitment to 3'-processing...
  8. pmc Imaging and characterizing influenza A virus mRNA transport in living cells
    Wei Wang
    State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, China
    Nucleic Acids Res 36:4913-28. 2008
    ..These findings characterize influenza A virus mRNA transport in living cells and suggest that influenza A virus mRNA may be exported from the nucleus by the cellular TAP/p15 pathway with NS1 protein and RNAP-II participation...
  9. pmc Ubiquitin-associated domain of Mex67 synchronizes recruitment of the mRNA export machinery with transcription
    Carole Gwizdek
    Institut Jacques Monod, Unite Mixte de Recherche 7592, Centre National de la Recherche Scientifique, Universités Paris VI and VII, 2 place Jussieu, Tour 43, 75251 Paris Cedex 05, France
    Proc Natl Acad Sci U S A 103:16376-81. 2006
    ..This interaction transiently protects Hpr1 from ubiquitin/proteasome-mediated degradation and thereby coordinates recruitment of the mRNA export machinery with transcription and early messenger ribonucleoproteins assembly...
  10. pmc Yra1p, a conserved nuclear RNA-binding protein, interacts directly with Mex67p and is required for mRNA export
    K Strasser
    BZH, Biochemie Zentrum Heidelberg, Germany
    EMBO J 19:410-20. 2000
    ..Thus, Yra1p is the first RNA-binding protein characterized, which bridges the shuttling Mex67p/Mtr2p exporter to intranuclear mRNA transport cargoes...
  11. ncbi VSV disrupts the Rae1/mrnp41 mRNA nuclear export pathway
    Paula A Faria
    Department of Molecular and Cellular Pharmacology, University of Miami School of Medicine, Miami, FL 33136, USA
    Mol Cell 17:93-102. 2005
    ..Thus, these results demonstrate that VSV M protein blocks mRNA export by disrupting Rae1 function, which can be reverted by induction of Rae1 expression...
  12. pmc Ubiquitin-mediated mRNP dynamics and surveillance prior to budding yeast mRNA export
    Nahid Iglesias
    Department of Cell Biology, Sciences III, 1211 Geneva 4, Switzerland
    Genes Dev 24:1927-38. 2010
    ....
  13. pmc The C-terminal domain of TAP interacts with the nuclear pore complex and promotes export of specific CTE-bearing RNA substrates
    A Bachi
    European Molecular Biology Laboratory, Heidelberg, Germany
    RNA 6:136-58. 2000
    ....
  14. ncbi PHAX, a mediator of U snRNA nuclear export whose activity is regulated by phosphorylation
    M Ohno
    European Molecular Biology Laboratory, Heidelberg, Germany
    Cell 101:187-98. 2000
    ..PHAX phosphorylation is essential for export complex assembly while its dephosphorylation causes export complex disassembly. The compartmentalized PHAX phosphorylation cycle can contribute to the directionality of export...
  15. pmc RNA content in the nucleolus alters p53 acetylation via MYBBP1A
    Takao Kuroda
    Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan
    EMBO J 30:1054-66. 2011
    ..Our observations indicated that a dynamic equilibrium between RNA generation and export regulated nucleolar RNA content. Perturbation of this balance by nucleolar stress altered the nucleolar RNA content and modulated p53 activity...
  16. pmc Phosphorylation of the eukaryotic translation initiation factor 4E-transporter (4E-T) by c-Jun N-terminal kinase promotes stress-dependent P-body assembly
    Marie Cargnello
    Institute for Research in Immunology and Cancer, Universite de Montreal, Montreal, Quebec, Canada
    Mol Cell Biol 32:4572-84. 2012
    ....
  17. ncbi Sus1, a functional component of the SAGA histone acetylase complex and the nuclear pore-associated mRNA export machinery
    Susana Rodriguez-Navarro
    Biochemie Zentrum der Universität Heidelberg BZH, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    Cell 116:75-86. 2004
    ..Finally, mRNA export is impaired in sus1 mutants. These data provide an unexpected connection between the SAGA histone acetylase complex and the mRNA export machinery...
  18. pmc Identification of Myb-binding protein 1A (MYBBP1A) as a novel substrate for aurora B kinase
    Claudia Perrera
    Nerviano Medical Sciences, Nerviano 20014 MI, Italy
    J Biol Chem 285:11775-85. 2010
    ..The results presented herein show MYBBP1A as a novel Aurora B kinase substrate and reveal a not yet recognized link of this nucleolar protein to mitosis...
  19. ncbi The DEAD-box protein Dbp5 controls mRNA export by triggering specific RNA:protein remodeling events
    Elizabeth J Tran
    Department of Cell and Developmental Biology, Vanderbilt University Medical Center, U 3209 MRBIII, 465 21st Avenue South, Nashville, TN 37232 8240, USA
    Mol Cell 28:850-9. 2007
    ..In vivo studies with nab2 and dbp5 mutants show that a Nab2-bound mRNP is a physiological Dbp5 target. We propose that Dbp5 functions as a nucleotide-dependent switch to control mRNA export efficiency and release the mRNP from the NPC...
  20. pmc Suppression of mitochondrial respiration through recruitment of p160 myb binding protein to PGC-1alpha: modulation by p38 MAPK
    Melina Fan
    Dana Farber Cancer Institute and the Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 18:278-89. 2004
    ..The discovery of p160MBP as a PGC-1alpha regulator has important implications for the understanding of energy balance and diabetes...
  21. pmc HRD4/NPL4 is required for the proteasomal processing of ubiquitinated ER proteins
    N W Bays
    Division of Biology, Section of Cell and Developmental Biology, University of California San Diego, La Jolla, CA 92093 0347, USA
    Mol Biol Cell 12:4114-28. 2001
    ..We also found that each member of the Cdc48p-Ufd1p-Npl4p complex is individually required for ERAD...
  22. pmc The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human
    J Katahira
    BZH, Biochemie Zentrum Heidelberg, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    EMBO J 18:2593-609. 1999
    ..Thus, the human TAP-p15 complex can functionally replace the Mex67p-Mtr2p complex in yeast and thus performs a conserved role in nuclear mRNA export...
  23. ncbi Nab2p and the Thp1p-Sac3p complex functionally interact at the interface between transcription and mRNA metabolism
    Mercedes Gallardo
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, Avenida Reina Mercedes 6, 41012 Sevilla, Spain
    J Biol Chem 278:24225-32. 2003
    ..Altogether, our results suggest that Thp1p-Sac3p and Nab2p are functionally related heterogeneous nuclear ribonucleoproteins that define a further link between mRNA metabolism and transcription...
  24. ncbi MYBBP1a is a novel repressor of NF-kappaB
    Heather R Owen
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    J Mol Biol 366:725-36. 2007
    ..These results indicate that MYBBP1a is a novel NF-kappaB co-repressor of transcription that competes with p300 and may function to regulate cell type specific genes...
  25. pmc mRNA export from mammalian cell nuclei is dependent on GANP
    Vihandha O Wickramasinghe
    MRC Cancer Cell Unit, Hutchison MRC Research Centre, Hills Road, Cambridge CB2 0XZ, UK
    Curr Biol 20:25-31. 2010
    ..These results establish GANP as an integral component of the mammalian mRNA export machinery and suggest a model whereby GANP facilitates the transfer of NXF1-containing mRNPs to NPCs...
  26. ncbi Ectopic expression of eIF4E-transporter triggers the movement of eIF4E into P-bodies, inhibiting steady-state translation but not the pioneer round of translation
    Hyung Chul Lee
    School of Life Sciences and Biotechnology, Korea University, Anam Dong, Seongbuk gu, Seoul 136 701, Republic of Korea
    Biochem Biophys Res Commun 369:1160-5. 2008
    ..These results suggest that the pioneer round of translation differs from steady-state translation in terms of ribosome recruitment...
  27. pmc The oncogene eIF4E reprograms the nuclear pore complex to promote mRNA export and oncogenic transformation
    Biljana Culjkovic-Kraljacic
    Institute for Research in Immunology and Cancer, Department of Pathology and Cell Biology, Universite de Montreal, Quebec, Canada
    Cell Rep 2:207-15. 2012
    ..eIF4E overcomes this inhibitory mechanism by indirectly reducing levels of RanBP2. More generally, these results suggest that reprogramming the NPC is a means by which oncogenes can harness the proliferative capacity of the cell...
  28. ncbi The human TREX-2 complex is stably associated with the nuclear pore basket
    David Umlauf
    Cellular Signaling and Nuclear Dynamics Program, Institut de Genetique et de Biologie Moleculaire et Cellulaire IGBMC, CNRS UMR 7104, INSERM U964, Universite de Strasbourg, BP 10142 67404 Illkirch Cedex, CU de Strasbourg, France
    J Cell Sci 126:2656-67. 2013
    ..Thus, our results show that TREX-2 is an NPC-associated complex in mammalian cells and suggest that it is involved in putative NPC basket-related functions. ..
  29. pmc mRNA binding protein mrnp 41 localizes to both nucleus and cytoplasm
    D Kraemer
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 94:9119-24. 1997
    ....
  30. pmc REF1/Aly and the additional exon junction complex proteins are dispensable for nuclear mRNA export
    David Gatfield
    European Molecular Biology Laboratory, 69117 Heidelberg, Germany
    J Cell Biol 159:579-88. 2002
    ..Our results imply that the essential role of UAP56 in mRNA export is not restricted to the recruitment of REF1...
  31. pmc The nuclear exosome contributes to autogenous control of NAB2 mRNA levels
    Kelly M Roth
    Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Box 672, 601 Elmwood Ave, Rochester, NY 14642, USA
    Mol Cell Biol 25:1577-85. 2005
    ..These findings suggest a novel regulatory circuit in which the nuclear exosome controls the level of NAB2 mRNA in response to changes in the activity of Nab2 protein...
  32. pmc Interaction between the shuttling mRNA export factor Gle1 and the nucleoporin hCG1: a conserved mechanism in the export of Hsp70 mRNA
    Frederic Kendirgi
    Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232 8240, USA
    Mol Biol Cell 16:4304-15. 2005
    ..Because this closely parallels the role of the hCG1 orthologue yNup42/Rip1, we speculate that hGle1-hCG1 function in the mRNA export mechanism is highly conserved...
  33. ncbi SRprises along a messenger's journey
    Yingqun Huang
    Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    Mol Cell 17:613-5. 2005
    ..Recent work, however, has implicated these proteins in numerous additional steps of mRNA metabolism, including nuclear export, RNA stability, mRNA quality control, and translation...
  34. pmc Novel nucleolar pathway connecting intracellular energy status with p53 activation
    Takuya Kumazawa
    Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba Science City, Ibaraki 305 8577, Japan
    J Biol Chem 286:20861-9. 2011
    ..Taken together, our results indicate that the nucleolus works as a sensor that transduces the intracellular energy status into the cell cycle machinery...
  35. ncbi The interaction between cap-binding complex and RNA export factor is required for intronless mRNA export
    Takayuki Nojima
    Laboratory of Gene Expression, School of Biomedical Science, Tokyo Medical and Dental University, Yushima 1 5 45, Bunkyo ku, Japan
    J Biol Chem 282:15645-51. 2007
    ..The export of Cy3-labeled intronless beta-globin mRNA from nuclei of HeLa cells was enhanced by co-injection of CBP20 and REF. Thus, REF recruited by CBP20 may play a stimulatory role to export the capped intronless mRNAs...
  36. pmc Selectivity mechanism of the nuclear pore complex characterized by single cargo tracking
    Alan R Lowe
    Department of Physics, University of California, Berkeley, California 94720, USA
    Nature 467:600-3. 2010
    ..The overall selectivity of the NPC seems to arise from the cumulative action of multiple reversible substeps and a final irreversible exit step...
  37. pmc The cellular RNA export receptor TAP/NXF1 is required for ICP27-mediated export of herpes simplex virus 1 RNA, but the TREX complex adaptor protein Aly/REF appears to be dispensable
    Lisa A Johnson
    Department of Microbiology and Molecular Genetics, School of Medicine, Medical Sciences I, Rm B240, University of California at Irvine, Irvine, CA 92697 4025, USA
    J Virol 83:6335-46. 2009
    ..In contrast, a decrease in TAP/NXF1 levels severely impaired export of ICP27 and poly(A)(+) RNA. We conclude that TAP/NXF1 is essential for ICP27-mediated export of RNA during HSV-1 infection, whereas Aly/REF may be dispensable...
  38. ncbi PHAX and CRM1 are required sequentially to transport U3 snoRNA to nucleoli
    Séverine Boulon
    IGMM, CNRS UMR 5535, IFR 122, 34293 Montpellier Cedex 5, France
    Mol Cell 16:777-87. 2004
    ..Our data indicate that the role of PHAX in determining the identity of small RNAs extends to nonexported species, and this appears critical to promote their transport within the nucleus...
  39. pmc Structural and functional analysis of the interaction between the nucleoporin Nup98 and the mRNA export factor Rae1
    Yi Ren
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 107:10406-11. 2010
    ..Together, these data suggest that the Rae1*Nup98 complex directly binds to the mRNP at several stages of the mRNA export pathway...
  40. ncbi The AAA ATPase Cdc48/p97 and its partners transport proteins from the ER into the cytosol
    Y Ye
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 414:652-6. 2001
    ..We propose that the Cdc48/p97-Ufd1-Npl4 complex extracts proteins from the ER membrane for cytosolic degradation...
  41. pmc Herpesvirus capsid association with the nuclear pore complex and viral DNA release involve the nucleoporin CAN/Nup214 and the capsid protein pUL25
    David Pasdeloup
    MRC Virology Unit, Church Street, Glasgow G11 5JR, United Kingdom
    J Virol 83:6610-23. 2009
    ....
  42. ncbi Solution and crystal structures of mRNA exporter Dbp5p and its interaction with nucleotides
    Jing Song Fan
    Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore
    J Mol Biol 388:1-10. 2009
    ..Binding of ATP or ADP to NTD induces significant conformational rearrangement too...
  43. pmc Binding of the Mex67p/Mtr2p heterodimer to FXFG, GLFG, and FG repeat nucleoporins is essential for nuclear mRNA export
    K Strasser
    BZH, Biochemie Zentrum Heidelberg, D 69120 Heidelberg, Germany
    J Cell Biol 150:695-706. 2000
    ....
  44. pmc A complex of mammalian ufd1 and npl4 links the AAA-ATPase, p97, to ubiquitin and nuclear transport pathways
    H H Meyer
    Department of Cell Biology, Yale University School of Medicine, 333 Cedar Street, SHM, C441, PO Box 208002, New Haven, CT 06520 8002, USA
    EMBO J 19:2181-92. 2000
    ..The fact that the binding of p47 and Ufd1/Npl4 is mutually exclusive suggests that these protein complexes act as adapters, directing a basic p97 activity into different cellular pathways...
  45. ncbi Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly
    M Hetzer
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nat Cell Biol 3:1086-91. 2001
    ..Subsequent NE growth involves a p97-p47 complex. This study provides the first insights into the molecular mechanisms and specificity of fusion events involved in NE formation...
  46. pmc Molecular and functional characterization of the p62 complex, an assembly of nuclear pore complex glycoproteins
    T Hu
    Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037, USA
    J Cell Biol 134:589-601. 1996
    ..These data support a model in which the p62 complex binds to a multicomponent particle consisting of transport ligand and cytosolic factors to achieve accumulation of ligand near the central gated channel of the nuclear pore complex...
  47. pmc Dual requirement for yeast hnRNP Nab2p in mRNA poly(A) tail length control and nuclear export
    Ronald E Hector
    Department of Molecular Genetics and Microbiology, Powell Gene Therapy Center, University of Florida College of Medicine, Gainesville, FL 32610 0266, USA
    EMBO J 21:1800-10. 2002
    ..We propose that Nab2p provides an important link between the termination of mRNA polyadenylation and nuclear export...
  48. pmc Rae1 interaction with NuMA is required for bipolar spindle formation
    Richard W Wong
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 103:19783-7. 2006
    ..These data point to the Rae1-NuMA interaction as a critical element for normal spindle formation in mitosis...
  49. pmc Characterization of Ran-driven cargo transport and the RanGTPase system by kinetic measurements and computer simulation
    Dirk Gorlich
    ZMBH, INF 282, 69120 Heidelberg, Germany
    EMBO J 22:1088-100. 2003
    ..This indicates that RanGTP gradients can provide positional information for mitotic spindle and NE assembly in early embryonic cells, but hardly any in small somatic cells...
  50. pmc Regulation of NAB2 mRNA 3'-end formation requires the core exosome and the Trf4p component of the TRAMP complex
    Kelly M Roth
    Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, New York 14642, USA
    RNA 15:1045-58. 2009
    ..These findings suggest that NAB2 mRNA 3'-end formation requires the exosome and TRAMP complex, and that competition between polyadenylation and Rrp6p-dependent degradation controls the level of this mRNA...
  51. pmc Functional specificity of shuttling hnRNPs revealed by genome-wide analysis of their RNA binding profiles
    Karen Kim Guisbert
    Department of Biochemistry and Biophysics, 600 16th Street, Genentech Hall, University of California at San Francisco, San Francisco, CA 94143, USA
    RNA 11:383-93. 2005
    ..These specialized associations of the hnRNP proteins of Saccharomyces cerevisiae suggest the opportunity to regulate the processing of particular transcripts between transcription and translation...
  52. pmc RanBP2/Nup358 provides a major binding site for NXF1-p15 dimers at the nuclear pore complex and functions in nuclear mRNA export
    Daniel Forler
    EMBL, 69117 Heidelberg, Germany
    Mol Cell Biol 24:1155-67. 2004
    ..In RanBP2-depleted cells, NXF1 diffuses freely through the cytoplasm. Consequently, the nuclear levels of the protein decrease and export of bulk mRNA is impaired...
  53. pmc Structural and functional analysis of RNA and TAP binding to SF2/ASF
    Aura M Tintaru
    Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK
    EMBO Rep 8:756-62. 2007
    ..The linker connecting RRM1 and RRM2 contains arginine residues, which provide a binding site for the mRNA export factor TAP, and when TAP binds to this region it displaces RNA bound to RRM2...
  54. ncbi Ribosomal stress induces processing of Mybbp1a and its translocation from the nucleolus to the nucleoplasm
    Tomohiro Yamauchi
    Laboratory of Molecular Genetics, RIKEN Tsukuba Institute, 3 1 1 Koyadai, Tsukuba, Ibaraki 305 0074, Japan
    Genes Cells 13:27-39. 2008
    ..These results suggest that Mybbp1a may connect the ribosome biogenesis and the Myb-dependent transcription, which controls cell cycle progression and proliferation...
  55. pmc YRA1 autoregulation requires nuclear export and cytoplasmic Edc3p-mediated degradation of its pre-mRNA
    Shuyun Dong
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    Mol Cell 25:559-73. 2007
    ....
  56. pmc eIF4A3 is a novel component of the exon junction complex
    Chia C Chan
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6148, USA
    RNA 10:200-9. 2004
    ..Using monoclonal antibodies, we show that eIF4A3 is found in the nucleus whereas eIF4A1 and eIF4A2 are found in the cytoplasm. Thus, eIF4A3 likely provides a splicing-dependent influence on the translation of mRNAs...
  57. ncbi Molecular cloning and chromosomal mapping of the human homologue of MYB binding protein (P160) 1A (MYBBP1A) to 17p13.3
    R Keough
    Hanson Centre for Cancer Research, Institute of Medical and Veterinary Science, Adelaide, South Australia, 5000, Australia
    Genomics 62:483-9. 1999
    ..A novel, polymorphic (CA)(25) dinucleotide repeat was also isolated from this PAC and may serve as a useful marker for MYBBP1A and this region of chromosome 17...
  58. ncbi Cex1p facilitates Rna1p-mediated dissociation of the Los1p-tRNA-Gsp1p-GTP export complex
    Andrew T McGuire
    Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, N1G 2W1, Canada
    Traffic 13:234-56. 2012
    ..It is possible that this tRNA unloading mechanism is conserved in evolutionarily diverse organisms and that other Gsp1p-GTP-dependent export processes use a pathway-specific component to recruit Rna1p to the NPC...
  59. ncbi The human RAE1 gene is a functional homologue of Schizosaccharomyces pombe rae1 gene involved in nuclear export of Poly(A)+ RNA
    A Bharathi
    Laboratory of Molecular Virology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Gene 198:251-8. 1997
    ..An epitope tagged human Rae1p localizes to both the nucleus and the cytoplasm in transiently transfected HeLa cells. We discuss the potential role of Rae1p in nuclear cytoplasmic trafficking in yeast and higher eukaryotic cells...
  60. pmc Opposing function of MYBBP1A in proliferation and migration of head and neck squamous cell carcinoma cells
    Gustavo A Acuña Sanhueza
    Department of Otolaryngology, Head and Neck Surgery, University Hospital Heidelberg, Heidelberg, Germany
    BMC Cancer 12:72. 2012
    ..Despite its significance, our knowledge on molecular, cellular and environmental mechanisms that drive disease pathogenesis remains largely elusive, and there are limited therapeutic options, with only negligible clinical benefit...
  61. pmc The long and the short of it: the role of the zinc finger polyadenosine RNA binding protein, Nab2, in control of poly(A) tail length
    Sharon Soucek
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    Biochim Biophys Acta 1819:546-54. 2012
    ..This article is part of a Special Issue entitled: Nuclear Transport and RNA Processing...
  62. ncbi Kap104p: a karyopherin involved in the nuclear transport of messenger RNA binding proteins
    J D Aitchison
    Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Science 274:624-7. 1996
    ..This finding suggests that the major function of Kap104p lies in returning mRNA binding proteins to the nucleus after mRNA export...
  63. pmc Karyopherin beta 2B participates in mRNA export from the nucleus
    Monee K Shamsher
    The Carl C Icahn Institute for Gene Therapy and Molecular Medicine, Mount Sinai School of Medicine, Box 1496, 1425 Madison Avenue, New York, NY 10029, USA
    Proc Natl Acad Sci U S A 99:14195-9. 2002
    ..The data support the conclusion that Kap beta2B participates directly in the export of a large proportion of cellular mRNAs, and TAP connects Kap beta2B to the mRNAs to be exported...
  64. pmc Cajal body surveillance of U snRNA export complex assembly
    Tatsuya Suzuki
    Institute for Virus Research, Kyoto University, Kyoto, Japan
    J Cell Biol 190:603-12. 2010
    ..Similar results were obtained with U snRNAs transcribed from microinjected genes. These results reveal a novel function for CBs, which ensure that U snRNA precursors are properly bound by PHAX...
  65. pmc Recognition of polyadenosine RNA by the zinc finger domain of nuclear poly(A) RNA-binding protein 2 (Nab2) is required for correct mRNA 3'-end formation
    Seth M Kelly
    Departments of Biochemistry, Emory University School ofMedicine, Atlanta, Georgia 30322, USA
    J Biol Chem 285:26022-32. 2010
    ..Together, these data provide strong evidence that Nab2 binding to RNA is critical for proper control of poly(A) tail length...
  66. pmc Arginine methylation of REF/ALY promotes efficient handover of mRNA to TAP/NXF1
    Ming Lung Hung
    Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK
    Nucleic Acids Res 38:3351-61. 2010
    ..Therefore, arginine methylation fine-tunes the RNA-binding activity of REF such that the RNA-protein interaction can be readily disrupted by export factors further down the pathway...
  67. pmc Identification of an NTF2-related factor that binds Ran-GTP and regulates nuclear protein export
    B E Black
    Center for Cell Signaling, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908, USA
    Mol Cell Biol 19:8616-24. 1999
    ..The export function of NXT1 is blocked by the addition of leptomycin B, a compound that selectively inhibits the NES receptor Crm1. Thus, NXT1 regulates the Crm1-dependent export pathway through its direct interaction with Ran-GTP...
  68. pmc An aminoacylation-dependent nuclear tRNA export pathway in yeast
    H Grosshans
    Biochemie Zentrum Heidelberg BZH, University of Heidelberg, D 69120 Heidelberg, Germany
    Genes Dev 14:830-40. 2000
    ..Our data show that tRNA aminoacylation and eEF-1A are required for efficient nuclear tRNA export in yeast and suggest coordination between the protein translation and the nuclear tRNA processing and transport machineries...
  69. ncbi FG-rich repeats of nuclear pore proteins form a three-dimensional meshwork with hydrogel-like properties
    Steffen Frey
    Zentrum für Molekulare Biologie der Universität Heidelberg ZMBH, INF 282, D 69120 Heidelberg, Germany
    Science 314:815-7. 2006
    ..Furthermore, we obtained evidence that such hydrogel formation is required for viability in yeast...
  70. pmc Molecular dissection of mRNA poly(A) tail length control in yeast
    Nicolas Viphakone
    Universite Victor Segalen Bordeaux 2, CNRS, Institut de Biochimie et Génétique Cellulaires, Bordeaux, France
    Nucleic Acids Res 36:2418-33. 2008
    ....
  71. pmc The DEXD/H-box RNA helicase DDX19 is regulated by an {alpha}-helical switch
    Ruairi Collins
    Structural Genomics Consortium, Department of Medical Biochemistry and Biophysics, Karolinska Institute, S 17177 Stockholm, Sweden
    J Biol Chem 284:10296-300. 2009
    ..This is the first study describing crystal structures of a DEXD/H-box protein in its open and closed cleft conformations...
  72. ncbi Regulation of mRNP dynamics along the export pathway
    Nahid Iglesias
    Department of Cell Biology, University of Geneva, 30 quai E Ansermet, 1211 Geneva 4, Switzerland
    FEBS Lett 582:1987-96. 2008
    ..Recent discoveries provide molecular understanding of how a broad range of post-translational modifications together with RNA-dependent ATPases coordinate proteins acting at different steps and regulate mRNP assembly and export...
  73. pmc Synthetic genetic array analysis in Saccharomyces cerevisiae provides evidence for an interaction between RAT8/DBP5 and genes encoding P-body components
    John J Scarcelli
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA
    Genetics 179:1945-55. 2008
    ..Although these foci are distinct from P-bodies, the two merge under heat-stress conditions. We suggest that these granules reflect defective mRNP remodeling during mRNA export and during cytoplasmic mRNA metabolism...
  74. pmc The Dbp5 cycle at the nuclear pore complex during mRNA export II: nucleotide cycling and mRNP remodeling by Dbp5 are controlled by Nup159 and Gle1
    Kristen N Noble
    Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Genes Dev 25:1065-77. 2011
    ..We propose a model wherein Nup159 and Gle1-IP(6) regulate Dbp5 cycles by controlling its nucleotide-bound state, allowing multiple cycles of mRNP remodeling by a single Dbp5 at the NPC...
  75. ncbi Characterization of the Drosophila Rae1 protein as a G1 phase regulator of the cell cycle
    D Sitterlin
    EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Gene 326:107-16. 2004
    ..Given that dmRae1 is closely related to the human form, results suggest that the human homologue, hsRAE1, may also play a similar role during the cell cycle...
  76. ncbi Critical role of the nucleolus in activation of the p53-dependent postmitotic checkpoint
    Mai Tsuchiya
    Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba Science City, Ibaraki 305 8572, Japan
    Biochem Biophys Res Commun 407:378-82. 2011
    ..Thus, our results demonstrate a novel nucleolar function that monitors the prolongation of mitosis and converts its signal into activation of the checkpoint machinery...
  77. pmc Nxf3 is expressed in Sertoli cells, but is dispensable for spermatogenesis
    Jian Zhou
    Department of Animal Biology, Center for Animal Transgenesis and Germ Cell Research, University of Pennsylvania School of Veterinary Medicine, Philadelphia, Pennsylvania 19104, USA
    Mol Reprod Dev 78:241-9. 2011
    ..With its unique expression pattern in the testis, the promoter of Nxf3 can be used to drive postnatal Sertoli cell-specific expression of other proteins such as Cre recombinase...
  78. ncbi A mutation in the Schizosaccharomyces pombe rae1 gene causes defects in poly(A)+ RNA export and in the cytoskeleton
    J A Brown
    Laboratory of Molecular Virology, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 270:7411-9. 1995
    ..The rae1 gene was isolated by complementation and encodes a predicted protein of 352 amino acids with four beta-transducin/WD40 repeats...
  79. ncbi Identification of a nuclear export receptor for tRNA
    G J Arts
    European Molecular Biology Laboratory, Heidelberg, Germany
    Curr Biol 8:305-14. 1998
    ..Export complexes form and import complexes disassemble on binding of RanGTP to the receptor. Yeast Los 1 p is a member of the importin-beta family with a poorly defined role in tRNA production...
  80. ncbi Conserved nuclear export sequences in Schizosaccharomyces pombe Mex67 and human TAP function in mRNA export by direct nuclear pore interactions
    Anjan G Thakurta
    Center for Cancer Research, NCI and NICHD, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 279:17434-42. 2004
    ..pombe strain, suggesting that the NESs can function in the absence of p15. These novel nuclear export sequences may provide additional routes for delivering Mex67/TAP to the nuclear pore complex...
  81. ncbi Ran-binding protein 3 links Crm1 to the Ran guanine nucleotide exchange factor
    Michael E Nemergut
    Department of Microbiology, The Center for Cell Signaling, The University of Virginia, Charlottesville, Virginia 22908, USA
    J Biol Chem 277:17385-8. 2002
    ..By tethering Crm1 to catalytically enhanced RCC1, RanBP3 may lower the entropic barrier for the loading of Ran.GTP onto Crm1. We propose that this provides an additional mechanism by which RanBP3 facilitates export...
  82. pmc Mex67p, a novel factor for nuclear mRNA export, binds to both poly(A)+ RNA and nuclear pores
    A Segref
    University of Heidelberg, BZH, Germany
    EMBO J 16:3256-71. 1997
    ..Thus, Mex67p is likely to participate directly in the export of mRNA from the nucleus to the cytoplasm...
  83. ncbi Myb-binding protein 1a is a nucleocytoplasmic shuttling protein that utilizes CRM1-dependent and independent nuclear export pathways
    Rebecca A Keough
    Hanson Institute, Division of Human Immunology, Institute of Medical and Veterinary Science, Adelaide, South Australia 5000, Australia
    Exp Cell Res 289:108-23. 2003
    ..Deletion analysis and in vivo export studies using a heterologous assay system identified several nuclear export sequences which facilitate Mybbp1a nuclear export of Mybbp1a by CRM1-dependent and -independent pathways...
  84. ncbi The GLFG regions of Nup116p and Nup100p serve as binding sites for both Kap95p and Mex67p at the nuclear pore complex
    L A Strawn
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 276:6445-52. 2001
    ..Taken together, our in vivo and in vitro results define an essential role for a direct Mex67p-GLFG interaction during mRNA export...
  85. ncbi Structural biology of nucleocytoplasmic transport
    Atlanta Cook
    European Molecular Biology Laboratory, D 69117 Heidelberg, Germany
    Annu Rev Biochem 76:647-71. 2007
    ....
  86. pmc The mRNA export machinery requires the novel Sac3p-Thp1p complex to dock at the nucleoplasmic entrance of the nuclear pores
    Tamas Fischer
    Biochemie Zentrum Heidelberg BZH, Im Neuenheimer Feld 328, Germany
    EMBO J 21:5843-52. 2002
    ..Significantly, mutations in Sac3p or Thp1p lead to strong mRNA export defects. Taken together, our data suggest that the novel Sac3p-Thp1p complex functions by docking the mRNP to specific nucleoporins at the nuclear entrance of the NPC...
  87. pmc A PY-NLS nuclear targeting signal is required for nuclear localization and function of the Saccharomyces cerevisiae mRNA-binding protein Hrp1
    Allison Lange
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 283:12926-34. 2008
    ..Finally, we apply this analysis to a bioinformatic search of the yeast proteome as a preliminary search for new potential Kap104 cargos...
  88. pmc Role of the ubiquitin-selective CDC48(UFD1/NPL4 )chaperone (segregase) in ERAD of OLE1 and other substrates
    Sigurd Braun
    Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    EMBO J 21:615-21. 2002
    ..We propose that CDC48(UFD1/NPL4) functions as a segregase that liberates ubiquitylated proteins from non-modified partners...
  89. pmc Nuclear export of Smad2 and Smad3 by RanBP3 facilitates termination of TGF-beta signaling
    Fangyan Dai
    Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Dev Cell 16:345-57. 2009
    ..In conclusion, our study supports a definitive role for RanBP3 in mediating Smad2/3 nuclear export and terminating TGF-beta signaling...
  90. pmc The role of exportin-t in selective nuclear export of mature tRNAs
    G J Arts
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    EMBO J 17:7430-41. 1998
    ..These results suggest that at least two mechanisms are involved in discrimination of pre-tRNAs and mature tRNAs prior to nuclear export...
  91. ncbi Protein dislocation from the ER requires polyubiquitination and the AAA-ATPase Cdc48
    Ernst Jarosch
    Max Delbruck Center for Molecular Medicine, Robert Rossle Strasse 10, 13092 Berlin, Germany
    Nat Cell Biol 4:134-9. 2002
    ..Moreover, our results suggest that the AAA proteins of the 26S proteasome are not directly involved in substrate export. Instead, a related AAA complex Cdc48, is required for ER-associated protein degradation upstream of the proteasome...
  92. pmc Structure-function studies of nucleocytoplasmic transport of retroviral genomic RNA by mRNA export factor TAP
    Marianna Teplova
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York, USA
    Nat Struct Mol Biol 18:990-8. 2011
    ....
  93. pmc Regulation of tRNA bidirectional nuclear-cytoplasmic trafficking in Saccharomyces cerevisiae
    Athulaprabha Murthi
    Department of Molecular Genetics, Graduate Program in Molecular, Cellular, and Developmental Biology, Center for RNA Biology, The Ohio State University, Columbus, OH 43210, USA
    Mol Biol Cell 21:639-49. 2010
    ..Finally, we implicate Tef1, the yeast orthologue of translation elongation factor eEF1A, in the tRNA reexport process and show that its subcellular distribution between the nucleus and cytoplasm is dependent upon Mtr10 and Msn5...
  94. pmc A specific subset of SR proteins shuttles continuously between the nucleus and the cytoplasm
    J F Caceres
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724 2208, USA
    Genes Dev 12:55-66. 1998
    ....
  95. pmc Mutations in tap uncouple RNA export activity from translocation through the nuclear pore complex
    LYNE LEVESQUE
    Department of Cell and Developmental Biology, University of Illinois in Urbana Champaign, Urbana, IL 61801, USA
    Mol Biol Cell 17:931-43. 2006
    ..These data suggest that Tap requires both the UBA- and NTF2-like domains to mediate the export of RNA cargo, but can move through the pores independently of these domains when free of RNA cargo...
  96. ncbi Structural basis for molecular recognition between nuclear transport factor 2 (NTF2) and the GDP-bound form of the Ras-family GTPase Ran
    M Stewart
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, England
    J Mol Biol 277:635-46. 1998
    ..The binding of GDP-Ran at the NTF2 cavity raises the possibility that this interaction might be modulated by a metabolite or small molecule substrate for NTF2's putative enzymatic activity...
  97. pmc Structural and functional analysis of the interaction between the nucleoporin Nup214 and the DEAD-box helicase Ddx19
    Johanna Napetschnig
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 106:3089-94. 2009
    ..Repeat of these cycles would remove proteins from a mRNP, one at a time, akin to a ratchet mechanism for mRNA export...
  98. pmc Structural similarity in the absence of sequence homology of the messenger RNA export factors Mtr2 and p15
    Sebastien Fribourg
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    EMBO Rep 4:699-703. 2003
    ..In addition, Mtr2 presents a long internal loop, which contains residues that affect the export of the large ribosomal subunit...
  99. ncbi Familial 1.1 Mb deletion in chromosome Xq22.1 associated with mental retardation and behavioural disorders in female patients
    L Grillo
    Laboratory of Genetic Diagnosis, I R C C S Associazione Oasi Maria Santissima, Troina, Italy
    Eur J Med Genet 53:113-6. 2010
    ..The dosage imbalance of NXF5 and NXF2 genes may explain the severe phenotype in our patient...
  100. ncbi Splicing factor Sub2p is required for nuclear mRNA export through its interaction with Yra1p
    K Strasser
    BZH Biochemie Zentrum Heidelberg, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    Nature 413:648-52. 2001
    ..Sub2p may then be displaced from Yra1p by the binding of Mex67p/Mtr2p, which participates in the export of mRNA through the nuclear pores...
  101. ncbi The evolutionarily conserved Kaposi's sarcoma-associated herpesvirus ORF57 protein interacts with REF protein and acts as an RNA export factor
    Poonam Malik
    Division of Virology, Institute of Biomedical and Life Sciences, University of Glasgow, Church Street, Glasgow, G11 5JR, Scotland, United Kingdom
    J Biol Chem 279:33001-11. 2004
    ..We propose that ORF57-mediated RNA export may use different export factors to accommodate the KSHV-infected host cell environments, for example, in B-cells or endothelial cells and during the different phases of lytic virus replication...

Research Grants11

  1. CRYO-EM STRUCTURAL STUDIES OF ADENOVIRUS CELL ENTRY
    Phoebe L Stewart; Fiscal Year: 2010
    ..Understanding the molecular principles of this natural defense mechanism could lead to novel strategies for antiviral drug design. ..
  2. Downstream Regulators of Beta-Amyloid Induced Neuronal Death
    Carol M Troy; Fiscal Year: 2010
    ..2: To determine how caspase-2 is regulated and activated by A_ and TFD. 3: To determine how caspase-2 induces of Bim expression. ..
  3. Chemical Genetic and Biochmechical Studies of Mitotic Proteolysis
    RANDALL KING; Fiscal Year: 2007
    ..abstract_text> ..
  4. Structure of Circadian Clock Complexes from Cyanobacteria by Three Dimensional EM
    Phoebe L Stewart; Fiscal Year: 2010
    ..abstract_text> ..
  5. Downstream Regulators of Beta-Amyloid Induced Neuronal Death
    CAROL TROY; Fiscal Year: 2008
    ..2: To determine how caspases are regulated and activated by A[unreadable] and TFD. 3: To determine how caspase-2 regulates the induction of Bim after A[unreadable] treatment. ..
  6. Regulation of Spermiogenesis in Mice
    PEIJING WANG; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  7. NEURONAL DEGENERATION: MECHANISMS AND PREVENTION
    CAROL TROY; Fiscal Year: 2007
    ..4. To determine how caspases-8 and -7 are activated after SOD1 down-regulation. 5. To determine if there is activation/regulation of caspase-3 and other caspases after SOD1 down-regulation. ..
  8. Influence of glycosylation on nuclear pore function
    KENNETH BELANGER; Fiscal Year: 2005
    ....
  9. Acquisition of a Liquid Helium Cryo-Electron Microscope
    PHOEBE STEWART; Fiscal Year: 2003
    ..The financial plan for the long term operation of the microscope includes a significant additional commitment from UCLA for microscope operating expenses. ..
  10. HERPES SIMPLEX VIRUS GENE REGULATION
    ROZANNE SANDRI GOLDIN; Fiscal Year: 2001
    ....
  11. Protein methyltransferases as transcription coactivators
    Michael Stallcup; Fiscal Year: 2007
    ..abstract_text> ..