nucleocytoplasmic transport proteins

Summary

Summary: Proteins involved in the process of transporting molecules in and out the cell nucleus. Included here are: NUCLEOPORINS, which are membrane proteins that form the NUCLEAR PORE COMPLEX; KARYOPHERINS, which carry molecules through the nuclear pore complex; and proteins that play a direct role in the transport of karyopherin complexes through the nuclear pore complex.

Top Publications

  1. pmc ATP is required for interactions between UAP56 and two conserved mRNA export proteins, Aly and CIP29, to assemble the TREX complex
    Kobina Dufu
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 24:2043-53. 2010
  2. pmc RNA content in the nucleolus alters p53 acetylation via MYBBP1A
    Takao Kuroda
    Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan
    EMBO J 30:1054-66. 2011
  3. pmc Yra1p, a conserved nuclear RNA-binding protein, interacts directly with Mex67p and is required for mRNA export
    K Strasser
    BZH, Biochemie Zentrum Heidelberg, Germany
    EMBO J 19:410-20. 2000
  4. pmc Arginine methylation of REF/ALY promotes efficient handover of mRNA to TAP/NXF1
    Ming Lung Hung
    Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK
    Nucleic Acids Res 38:3351-61. 2010
  5. pmc Identification of Myb-binding protein 1A (MYBBP1A) as a novel substrate for aurora B kinase
    Claudia Perrera
    Nerviano Medical Sciences, Nerviano 20014 MI, Italy
    J Biol Chem 285:11775-85. 2010
  6. pmc Suppression of mitochondrial respiration through recruitment of p160 myb binding protein to PGC-1alpha: modulation by p38 MAPK
    Melina Fan
    Dana Farber Cancer Institute and the Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 18:278-89. 2004
  7. pmc mRNA export from mammalian cell nuclei is dependent on GANP
    Vihandha O Wickramasinghe
    MRC Cancer Cell Unit, Hutchison MRC Research Centre, Hills Road, Cambridge CB2 0XZ, UK
    Curr Biol 20:25-31. 2010
  8. ncbi MYBBP1a is a novel repressor of NF-kappaB
    Heather R Owen
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    J Mol Biol 366:725-36. 2007
  9. ncbi The human RAE1 gene is a functional homologue of Schizosaccharomyces pombe rae1 gene involved in nuclear export of Poly(A)+ RNA
    A Bharathi
    Laboratory of Molecular Virology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Gene 198:251-8. 1997
  10. pmc Ubiquitin-associated domain of Mex67 synchronizes recruitment of the mRNA export machinery with transcription
    Carole Gwizdek
    Institut Jacques Monod, Unite Mixte de Recherche 7592, Centre National de la Recherche Scientifique, Universités Paris VI and VII, 2 place Jussieu, Tour 43, 75251 Paris Cedex 05, France
    Proc Natl Acad Sci U S A 103:16376-81. 2006

Research Grants

Detail Information

Publications206 found, 100 shown here

  1. pmc ATP is required for interactions between UAP56 and two conserved mRNA export proteins, Aly and CIP29, to assemble the TREX complex
    Kobina Dufu
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 24:2043-53. 2010
    ..We conclude that the interaction of two conserved export proteins, CIP29 and Aly, with UAP56 is strictly regulated by ATP during assembly of the TREX complex...
  2. pmc RNA content in the nucleolus alters p53 acetylation via MYBBP1A
    Takao Kuroda
    Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan
    EMBO J 30:1054-66. 2011
    ..Our observations indicated that a dynamic equilibrium between RNA generation and export regulated nucleolar RNA content. Perturbation of this balance by nucleolar stress altered the nucleolar RNA content and modulated p53 activity...
  3. pmc Yra1p, a conserved nuclear RNA-binding protein, interacts directly with Mex67p and is required for mRNA export
    K Strasser
    BZH, Biochemie Zentrum Heidelberg, Germany
    EMBO J 19:410-20. 2000
    ..Thus, Yra1p is the first RNA-binding protein characterized, which bridges the shuttling Mex67p/Mtr2p exporter to intranuclear mRNA transport cargoes...
  4. pmc Arginine methylation of REF/ALY promotes efficient handover of mRNA to TAP/NXF1
    Ming Lung Hung
    Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK
    Nucleic Acids Res 38:3351-61. 2010
    ..Therefore, arginine methylation fine-tunes the RNA-binding activity of REF such that the RNA-protein interaction can be readily disrupted by export factors further down the pathway...
  5. pmc Identification of Myb-binding protein 1A (MYBBP1A) as a novel substrate for aurora B kinase
    Claudia Perrera
    Nerviano Medical Sciences, Nerviano 20014 MI, Italy
    J Biol Chem 285:11775-85. 2010
    ..The results presented herein show MYBBP1A as a novel Aurora B kinase substrate and reveal a not yet recognized link of this nucleolar protein to mitosis...
  6. pmc Suppression of mitochondrial respiration through recruitment of p160 myb binding protein to PGC-1alpha: modulation by p38 MAPK
    Melina Fan
    Dana Farber Cancer Institute and the Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 18:278-89. 2004
    ..The discovery of p160MBP as a PGC-1alpha regulator has important implications for the understanding of energy balance and diabetes...
  7. pmc mRNA export from mammalian cell nuclei is dependent on GANP
    Vihandha O Wickramasinghe
    MRC Cancer Cell Unit, Hutchison MRC Research Centre, Hills Road, Cambridge CB2 0XZ, UK
    Curr Biol 20:25-31. 2010
    ..These results establish GANP as an integral component of the mammalian mRNA export machinery and suggest a model whereby GANP facilitates the transfer of NXF1-containing mRNPs to NPCs...
  8. ncbi MYBBP1a is a novel repressor of NF-kappaB
    Heather R Owen
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    J Mol Biol 366:725-36. 2007
    ..These results indicate that MYBBP1a is a novel NF-kappaB co-repressor of transcription that competes with p300 and may function to regulate cell type specific genes...
  9. ncbi The human RAE1 gene is a functional homologue of Schizosaccharomyces pombe rae1 gene involved in nuclear export of Poly(A)+ RNA
    A Bharathi
    Laboratory of Molecular Virology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Gene 198:251-8. 1997
    ..An epitope tagged human Rae1p localizes to both the nucleus and the cytoplasm in transiently transfected HeLa cells. We discuss the potential role of Rae1p in nuclear cytoplasmic trafficking in yeast and higher eukaryotic cells...
  10. pmc Ubiquitin-associated domain of Mex67 synchronizes recruitment of the mRNA export machinery with transcription
    Carole Gwizdek
    Institut Jacques Monod, Unite Mixte de Recherche 7592, Centre National de la Recherche Scientifique, Universités Paris VI and VII, 2 place Jussieu, Tour 43, 75251 Paris Cedex 05, France
    Proc Natl Acad Sci U S A 103:16376-81. 2006
    ..This interaction transiently protects Hpr1 from ubiquitin/proteasome-mediated degradation and thereby coordinates recruitment of the mRNA export machinery with transcription and early messenger ribonucleoproteins assembly...
  11. ncbi The mRNA export protein DBP5 binds RNA and the cytoplasmic nucleoporin NUP214 in a mutually exclusive manner
    Holger von Moeller
    Max Planck Institute of Biochemistry, Department of Structural Cell Biology, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Nat Struct Mol Biol 16:247-54. 2009
    ..More generally, they provide a paradigm for how binding of regulators can specifically inhibit DEAD-box proteins...
  12. pmc Opposing function of MYBBP1A in proliferation and migration of head and neck squamous cell carcinoma cells
    Gustavo A Acuña Sanhueza
    Department of Otolaryngology, Head and Neck Surgery, University Hospital Heidelberg, Heidelberg, Germany
    BMC Cancer 12:72. 2012
    ..Despite its significance, our knowledge on molecular, cellular and environmental mechanisms that drive disease pathogenesis remains largely elusive, and there are limited therapeutic options, with only negligible clinical benefit...
  13. ncbi SRprises along a messenger's journey
    Yingqun Huang
    Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    Mol Cell 17:613-5. 2005
    ..Recent work, however, has implicated these proteins in numerous additional steps of mRNA metabolism, including nuclear export, RNA stability, mRNA quality control, and translation...
  14. pmc Identification of mRNAs that are spliced but not exported to the cytoplasm in the absence of THOC5 in mouse embryo fibroblasts
    Anuja Guria
    Institut fur Biochemie, OE4310, Medizinische Hochschule Hannover, D 30623 Hannover, Germany
    RNA 17:1048-56. 2011
    ..Furthermore, Hsp70 mRNA was exported in the absence of THOC5 at 37°C, but not under heat shock condition (42°C), suggesting that THOC5 may be required for mRNA export under stress and/or upon signaling-induced conditions...
  15. pmc Novel nucleolar pathway connecting intracellular energy status with p53 activation
    Takuya Kumazawa
    Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba Science City, Ibaraki 305 8577, Japan
    J Biol Chem 286:20861-9. 2011
    ..Taken together, our results indicate that the nucleolus works as a sensor that transduces the intracellular energy status into the cell cycle machinery...
  16. pmc YRA1 autoregulation requires nuclear export and cytoplasmic Edc3p-mediated degradation of its pre-mRNA
    Shuyun Dong
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    Mol Cell 25:559-73. 2007
    ....
  17. pmc Binding of the Mex67p/Mtr2p heterodimer to FXFG, GLFG, and FG repeat nucleoporins is essential for nuclear mRNA export
    K Strasser
    BZH, Biochemie Zentrum Heidelberg, D 69120 Heidelberg, Germany
    J Cell Biol 150:695-706. 2000
    ....
  18. pmc A complex of mammalian ufd1 and npl4 links the AAA-ATPase, p97, to ubiquitin and nuclear transport pathways
    H H Meyer
    Department of Cell Biology, Yale University School of Medicine, 333 Cedar Street, SHM, C441, PO Box 208002, New Haven, CT 06520 8002, USA
    EMBO J 19:2181-92. 2000
    ..The fact that the binding of p47 and Ufd1/Npl4 is mutually exclusive suggests that these protein complexes act as adapters, directing a basic p97 activity into different cellular pathways...
  19. pmc Herpesvirus capsid association with the nuclear pore complex and viral DNA release involve the nucleoporin CAN/Nup214 and the capsid protein pUL25
    David Pasdeloup
    MRC Virology Unit, Church Street, Glasgow G11 5JR, United Kingdom
    J Virol 83:6610-23. 2009
    ....
  20. ncbi The AAA ATPase Cdc48/p97 and its partners transport proteins from the ER into the cytosol
    Y Ye
    Howard Hughes Medical Institute and Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 414:652-6. 2001
    ..We propose that the Cdc48/p97-Ufd1-Npl4 complex extracts proteins from the ER membrane for cytosolic degradation...
  21. pmc Structural and functional analysis of the interaction between the nucleoporin Nup98 and the mRNA export factor Rae1
    Yi Ren
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 107:10406-11. 2010
    ..Together, these data suggest that the Rae1*Nup98 complex directly binds to the mRNP at several stages of the mRNA export pathway...
  22. doi Solution and crystal structures of mRNA exporter Dbp5p and its interaction with nucleotides
    Jing Song Fan
    Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore
    J Mol Biol 388:1-10. 2009
    ..Binding of ATP or ADP to NTD induces significant conformational rearrangement too...
  23. ncbi Nab2p and the Thp1p-Sac3p complex functionally interact at the interface between transcription and mRNA metabolism
    Mercedes Gallardo
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, Avenida Reina Mercedes 6, 41012 Sevilla, Spain
    J Biol Chem 278:24225-32. 2003
    ..Altogether, our results suggest that Thp1p-Sac3p and Nab2p are functionally related heterogeneous nuclear ribonucleoproteins that define a further link between mRNA metabolism and transcription...
  24. ncbi Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly
    M Hetzer
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Nat Cell Biol 3:1086-91. 2001
    ..Subsequent NE growth involves a p97-p47 complex. This study provides the first insights into the molecular mechanisms and specificity of fusion events involved in NE formation...
  25. ncbi VSV disrupts the Rae1/mrnp41 mRNA nuclear export pathway
    Paula A Faria
    Department of Molecular and Cellular Pharmacology, University of Miami School of Medicine, Miami, FL 33136, USA
    Mol Cell 17:93-102. 2005
    ..Thus, these results demonstrate that VSV M protein blocks mRNA export by disrupting Rae1 function, which can be reverted by induction of Rae1 expression...
  26. doi Ribosomal stress induces processing of Mybbp1a and its translocation from the nucleolus to the nucleoplasm
    Tomohiro Yamauchi
    Laboratory of Molecular Genetics, RIKEN Tsukuba Institute, 3 1 1 Koyadai, Tsukuba, Ibaraki 305 0074, Japan
    Genes Cells 13:27-39. 2008
    ..These results suggest that Mybbp1a may connect the ribosome biogenesis and the Myb-dependent transcription, which controls cell cycle progression and proliferation...
  27. pmc Molecular dissection of mRNA poly(A) tail length control in yeast
    Nicolas Viphakone
    Universite Victor Segalen Bordeaux 2, CNRS, Institut de Biochimie et Génétique Cellulaires, Bordeaux, France
    Nucleic Acids Res 36:2418-33. 2008
    ....
  28. pmc RanBP2/Nup358 provides a major binding site for NXF1-p15 dimers at the nuclear pore complex and functions in nuclear mRNA export
    Daniel Forler
    EMBL, 69117 Heidelberg, Germany
    Mol Cell Biol 24:1155-67. 2004
    ..In RanBP2-depleted cells, NXF1 diffuses freely through the cytoplasm. Consequently, the nuclear levels of the protein decrease and export of bulk mRNA is impaired...
  29. pmc Structural and functional analysis of RNA and TAP binding to SF2/ASF
    Aura M Tintaru
    Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK
    EMBO Rep 8:756-62. 2007
    ..The linker connecting RRM1 and RRM2 contains arginine residues, which provide a binding site for the mRNA export factor TAP, and when TAP binds to this region it displaces RNA bound to RRM2...
  30. ncbi Characterization of the Drosophila Rae1 protein as a G1 phase regulator of the cell cycle
    D Sitterlin
    EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Gene 326:107-16. 2004
    ..Given that dmRae1 is closely related to the human form, results suggest that the human homologue, hsRAE1, may also play a similar role during the cell cycle...
  31. pmc Synthetic genetic array analysis in Saccharomyces cerevisiae provides evidence for an interaction between RAT8/DBP5 and genes encoding P-body components
    John J Scarcelli
    Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA
    Genetics 179:1945-55. 2008
    ..Although these foci are distinct from P-bodies, the two merge under heat-stress conditions. We suggest that these granules reflect defective mRNP remodeling during mRNA export and during cytoplasmic mRNA metabolism...
  32. ncbi A mutation in the Schizosaccharomyces pombe rae1 gene causes defects in poly(A)+ RNA export and in the cytoskeleton
    J A Brown
    Laboratory of Molecular Virology, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 270:7411-9. 1995
    ..The rae1 gene was isolated by complementation and encodes a predicted protein of 352 amino acids with four beta-transducin/WD40 repeats...
  33. pmc The Dbp5 cycle at the nuclear pore complex during mRNA export II: nucleotide cycling and mRNP remodeling by Dbp5 are controlled by Nup159 and Gle1
    Kristen N Noble
    Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Genes Dev 25:1065-77. 2011
    ..We propose a model wherein Nup159 and Gle1-IP(6) regulate Dbp5 cycles by controlling its nucleotide-bound state, allowing multiple cycles of mRNP remodeling by a single Dbp5 at the NPC...
  34. pmc mRNA binding protein mrnp 41 localizes to both nucleus and cytoplasm
    D Kraemer
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 94:9119-24. 1997
    ....
  35. pmc Nxf3 is expressed in Sertoli cells, but is dispensable for spermatogenesis
    Jian Zhou
    Department of Animal Biology, Center for Animal Transgenesis and Germ Cell Research, University of Pennsylvania School of Veterinary Medicine, Philadelphia, Pennsylvania 19104, USA
    Mol Reprod Dev 78:241-9. 2011
    ..With its unique expression pattern in the testis, the promoter of Nxf3 can be used to drive postnatal Sertoli cell-specific expression of other proteins such as Cre recombinase...
  36. pmc The DEXD/H-box RNA helicase DDX19 is regulated by an {alpha}-helical switch
    Ruairi Collins
    Structural Genomics Consortium, Department of Medical Biochemistry and Biophysics, Karolinska Institute, S 17177 Stockholm, Sweden
    J Biol Chem 284:10296-300. 2009
    ..This is the first study describing crystal structures of a DEXD/H-box protein in its open and closed cleft conformations...
  37. doi Critical role of the nucleolus in activation of the p53-dependent postmitotic checkpoint
    Mai Tsuchiya
    Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba Science City, Ibaraki 305 8572, Japan
    Biochem Biophys Res Commun 407:378-82. 2011
    ..Thus, our results demonstrate a novel nucleolar function that monitors the prolongation of mitosis and converts its signal into activation of the checkpoint machinery...
  38. doi Regulation of mRNP dynamics along the export pathway
    Nahid Iglesias
    Department of Cell Biology, University of Geneva, 30 quai E Ansermet, 1211 Geneva 4, Switzerland
    FEBS Lett 582:1987-96. 2008
    ..Recent discoveries provide molecular understanding of how a broad range of post-translational modifications together with RNA-dependent ATPases coordinate proteins acting at different steps and regulate mRNP assembly and export...
  39. ncbi Identification of a nuclear export receptor for tRNA
    G J Arts
    European Molecular Biology Laboratory, Heidelberg, Germany
    Curr Biol 8:305-14. 1998
    ..Export complexes form and import complexes disassemble on binding of RanGTP to the receptor. Yeast Los 1 p is a member of the importin-beta family with a poorly defined role in tRNA production...
  40. ncbi Ran-binding protein 3 links Crm1 to the Ran guanine nucleotide exchange factor
    Michael E Nemergut
    Department of Microbiology, The Center for Cell Signaling, The University of Virginia, Charlottesville, Virginia 22908, USA
    J Biol Chem 277:17385-8. 2002
    ..By tethering Crm1 to catalytically enhanced RCC1, RanBP3 may lower the entropic barrier for the loading of Ran.GTP onto Crm1. We propose that this provides an additional mechanism by which RanBP3 facilitates export...
  41. pmc HRD4/NPL4 is required for the proteasomal processing of ubiquitinated ER proteins
    N W Bays
    Division of Biology, Section of Cell and Developmental Biology, University of California San Diego, La Jolla, CA 92093 0347, USA
    Mol Biol Cell 12:4114-28. 2001
    ..We also found that each member of the Cdc48p-Ufd1p-Npl4p complex is individually required for ERAD...
  42. ncbi Conserved nuclear export sequences in Schizosaccharomyces pombe Mex67 and human TAP function in mRNA export by direct nuclear pore interactions
    Anjan G Thakurta
    Center for Cancer Research, NCI and NICHD, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 279:17434-42. 2004
    ..pombe strain, suggesting that the NESs can function in the absence of p15. These novel nuclear export sequences may provide additional routes for delivering Mex67/TAP to the nuclear pore complex...
  43. ncbi Myb-binding protein 1a is a nucleocytoplasmic shuttling protein that utilizes CRM1-dependent and independent nuclear export pathways
    Rebecca A Keough
    Hanson Institute, Division of Human Immunology, Institute of Medical and Veterinary Science, Adelaide, South Australia 5000, Australia
    Exp Cell Res 289:108-23. 2003
    ..Deletion analysis and in vivo export studies using a heterologous assay system identified several nuclear export sequences which facilitate Mybbp1a nuclear export of Mybbp1a by CRM1-dependent and -independent pathways...
  44. pmc The C-terminal domain of TAP interacts with the nuclear pore complex and promotes export of specific CTE-bearing RNA substrates
    A Bachi
    European Molecular Biology Laboratory, Heidelberg, Germany
    RNA 6:136-58. 2000
    ....
  45. ncbi Structural biology of nucleocytoplasmic transport
    Atlanta Cook
    European Molecular Biology Laboratory, D 69117 Heidelberg, Germany
    Annu Rev Biochem 76:647-71. 2007
    ....
  46. pmc The mRNA export machinery requires the novel Sac3p-Thp1p complex to dock at the nucleoplasmic entrance of the nuclear pores
    Tamas Fischer
    Biochemie Zentrum Heidelberg BZH, Im Neuenheimer Feld 328, Germany
    EMBO J 21:5843-52. 2002
    ..Significantly, mutations in Sac3p or Thp1p lead to strong mRNA export defects. Taken together, our data suggest that the novel Sac3p-Thp1p complex functions by docking the mRNP to specific nucleoporins at the nuclear entrance of the NPC...
  47. ncbi The GLFG regions of Nup116p and Nup100p serve as binding sites for both Kap95p and Mex67p at the nuclear pore complex
    L A Strawn
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 276:6445-52. 2001
    ..Taken together, our in vivo and in vitro results define an essential role for a direct Mex67p-GLFG interaction during mRNA export...
  48. pmc An aminoacylation-dependent nuclear tRNA export pathway in yeast
    H Grosshans
    Biochemie Zentrum Heidelberg BZH, University of Heidelberg, D 69120 Heidelberg, Germany
    Genes Dev 14:830-40. 2000
    ..Our data show that tRNA aminoacylation and eEF-1A are required for efficient nuclear tRNA export in yeast and suggest coordination between the protein translation and the nuclear tRNA processing and transport machineries...
  49. pmc A PY-NLS nuclear targeting signal is required for nuclear localization and function of the Saccharomyces cerevisiae mRNA-binding protein Hrp1
    Allison Lange
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 283:12926-34. 2008
    ..Finally, we apply this analysis to a bioinformatic search of the yeast proteome as a preliminary search for new potential Kap104 cargos...
  50. pmc Adaptor Aly and co-adaptor Thoc5 function in the Tap-p15-mediated nuclear export of HSP70 mRNA
    Jun Katahira
    Biomolecular Dynamics Laboratory, Graduate School of Frontier Biosciences, Osaka University, Yamadaoka, Suita, Osaka, Japan Japan
    EMBO J 28:556-67. 2009
    ..Thus, through the combinatorial use of an adaptor (e.g., Aly) and co-adapter (e.g., Thoc5), Tap-p15 could function as an export receptor for different classes of mRNAs...
  51. pmc Nuclear export of Smad2 and Smad3 by RanBP3 facilitates termination of TGF-beta signaling
    Fangyan Dai
    Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Dev Cell 16:345-57. 2009
    ..In conclusion, our study supports a definitive role for RanBP3 in mediating Smad2/3 nuclear export and terminating TGF-beta signaling...
  52. pmc Role of the ubiquitin-selective CDC48(UFD1/NPL4 )chaperone (segregase) in ERAD of OLE1 and other substrates
    Sigurd Braun
    Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    EMBO J 21:615-21. 2002
    ..We propose that CDC48(UFD1/NPL4) functions as a segregase that liberates ubiquitylated proteins from non-modified partners...
  53. ncbi Protein dislocation from the ER requires polyubiquitination and the AAA-ATPase Cdc48
    Ernst Jarosch
    Max Delbruck Center for Molecular Medicine, Robert Rossle Strasse 10, 13092 Berlin, Germany
    Nat Cell Biol 4:134-9. 2002
    ..Moreover, our results suggest that the AAA proteins of the 26S proteasome are not directly involved in substrate export. Instead, a related AAA complex Cdc48, is required for ER-associated protein degradation upstream of the proteasome...
  54. pmc The role of exportin-t in selective nuclear export of mature tRNAs
    G J Arts
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    EMBO J 17:7430-41. 1998
    ..These results suggest that at least two mechanisms are involved in discrimination of pre-tRNAs and mature tRNAs prior to nuclear export...
  55. pmc Regulation of tRNA bidirectional nuclear-cytoplasmic trafficking in Saccharomyces cerevisiae
    Athulaprabha Murthi
    Department of Molecular Genetics, Graduate Program in Molecular, Cellular, and Developmental Biology, Center for RNA Biology, The Ohio State University, Columbus, OH 43210, USA
    Mol Biol Cell 21:639-49. 2010
    ..Finally, we implicate Tef1, the yeast orthologue of translation elongation factor eEF1A, in the tRNA reexport process and show that its subcellular distribution between the nucleus and cytoplasm is dependent upon Mtr10 and Msn5...
  56. pmc Structure-function studies of nucleocytoplasmic transport of retroviral genomic RNA by mRNA export factor TAP
    Marianna Teplova
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York, USA
    Nat Struct Mol Biol 18:990-8. 2011
    ....
  57. pmc A specific subset of SR proteins shuttles continuously between the nucleus and the cytoplasm
    J F Caceres
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724 2208, USA
    Genes Dev 12:55-66. 1998
    ....
  58. pmc Structural and functional analysis of the interaction between the nucleoporin Nup214 and the DEAD-box helicase Ddx19
    Johanna Napetschnig
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 106:3089-94. 2009
    ..Repeat of these cycles would remove proteins from a mRNP, one at a time, akin to a ratchet mechanism for mRNA export...
  59. ncbi Structural basis for molecular recognition between nuclear transport factor 2 (NTF2) and the GDP-bound form of the Ras-family GTPase Ran
    M Stewart
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, England
    J Mol Biol 277:635-46. 1998
    ..The binding of GDP-Ran at the NTF2 cavity raises the possibility that this interaction might be modulated by a metabolite or small molecule substrate for NTF2's putative enzymatic activity...
  60. pmc Mutations in tap uncouple RNA export activity from translocation through the nuclear pore complex
    LYNE LEVESQUE
    Department of Cell and Developmental Biology, University of Illinois in Urbana Champaign, Urbana, IL 61801, USA
    Mol Biol Cell 17:931-43. 2006
    ..These data suggest that Tap requires both the UBA- and NTF2-like domains to mediate the export of RNA cargo, but can move through the pores independently of these domains when free of RNA cargo...
  61. pmc Structural similarity in the absence of sequence homology of the messenger RNA export factors Mtr2 and p15
    Sebastien Fribourg
    European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    EMBO Rep 4:699-703. 2003
    ..In addition, Mtr2 presents a long internal loop, which contains residues that affect the export of the large ribosomal subunit...
  62. pmc The cellular RNA export receptor TAP/NXF1 is required for ICP27-mediated export of herpes simplex virus 1 RNA, but the TREX complex adaptor protein Aly/REF appears to be dispensable
    Lisa A Johnson
    Department of Microbiology and Molecular Genetics, School of Medicine, Medical Sciences I, Rm B240, University of California at Irvine, Irvine, CA 92697 4025, USA
    J Virol 83:6335-46. 2009
    ..In contrast, a decrease in TAP/NXF1 levels severely impaired export of ICP27 and poly(A)(+) RNA. We conclude that TAP/NXF1 is essential for ICP27-mediated export of RNA during HSV-1 infection, whereas Aly/REF may be dispensable...
  63. pmc Mutually exclusive interactions drive handover of mRNA from export adaptors to TAP
    Guillaume M Hautbergue
    Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, United Kingdom
    Proc Natl Acad Sci U S A 105:5154-9. 2008
    ..The importance of direct TAP-mRNA interactions is confirmed by the observation that a mutant form of TAP that fails to bind mRNA but retains the ability to bind REF does not function in mRNA export...
  64. ncbi The DEAD-box protein Dbp5 controls mRNA export by triggering specific RNA:protein remodeling events
    Elizabeth J Tran
    Department of Cell and Developmental Biology, Vanderbilt University Medical Center, U 3209 MRBIII, 465 21st Avenue South, Nashville, TN 37232 8240, USA
    Mol Cell 28:850-9. 2007
    ..In vivo studies with nab2 and dbp5 mutants show that a Nab2-bound mRNP is a physiological Dbp5 target. We propose that Dbp5 functions as a nucleotide-dependent switch to control mRNA export efficiency and release the mRNP from the NPC...
  65. ncbi Cex1p facilitates Rna1p-mediated dissociation of the Los1p-tRNA-Gsp1p-GTP export complex
    Andrew T McGuire
    Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, N1G 2W1, Canada
    Traffic 13:234-56. 2012
    ..It is possible that this tRNA unloading mechanism is conserved in evolutionarily diverse organisms and that other Gsp1p-GTP-dependent export processes use a pathway-specific component to recruit Rna1p to the NPC...
  66. ncbi The evolutionarily conserved Kaposi's sarcoma-associated herpesvirus ORF57 protein interacts with REF protein and acts as an RNA export factor
    Poonam Malik
    Division of Virology, Institute of Biomedical and Life Sciences, University of Glasgow, Church Street, Glasgow, G11 5JR, Scotland, United Kingdom
    J Biol Chem 279:33001-11. 2004
    ..We propose that ORF57-mediated RNA export may use different export factors to accommodate the KSHV-infected host cell environments, for example, in B-cells or endothelial cells and during the different phases of lytic virus replication...
  67. pmc Formation of a Tap/NXF1 homotypic complex is mediated through the amino-terminal domain of Tap and enhances interaction with nucleoporins
    Leah H Matzat
    Department of Cell and Developmental Biology, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Mol Biol Cell 19:327-38. 2008
    ..This is the first report showing that the Tap amino terminus modulates the interaction of Tap with nucleoporins. We speculate that this mechanism has a regulatory role for RNA export independent of RNA binding...
  68. ncbi The Mtr2-Mex67 NTF2-like domain complex. Structural insights into a dual role of Mtr2 for yeast nuclear export
    Claire Senay
    AFMB CNRS, UMR 6098, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Biol Chem 278:48395-403. 2003
    ....
  69. ncbi Advancement through mitosis requires rae1 gene function in fission yeast
    W A Whalen
    Laboratory of Molecular Virology, National Cancer Institute, Bethesda, MD 20892, USA
    Yeast 13:1167-79. 1997
    ..We propose a model where rae1 functions in cell cycle progression through trafficking of proteins required for mitosis...
  70. doi Familial 1.1 Mb deletion in chromosome Xq22.1 associated with mental retardation and behavioural disorders in female patients
    L Grillo
    Laboratory of Genetic Diagnosis, I R C C S Associazione Oasi Maria Santissima, Troina, Italy
    Eur J Med Genet 53:113-6. 2010
    ..The dosage imbalance of NXF5 and NXF2 genes may explain the severe phenotype in our patient...
  71. pmc Recognition of polyadenosine RNA by the zinc finger domain of nuclear poly(A) RNA-binding protein 2 (Nab2) is required for correct mRNA 3'-end formation
    Seth M Kelly
    Departments of Biochemistry, Emory University School ofMedicine, Atlanta, Georgia 30322, USA
    J Biol Chem 285:26022-32. 2010
    ..Together, these data provide strong evidence that Nab2 binding to RNA is critical for proper control of poly(A) tail length...
  72. ncbi Splicing factor Sub2p is required for nuclear mRNA export through its interaction with Yra1p
    K Strasser
    BZH Biochemie Zentrum Heidelberg, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    Nature 413:648-52. 2001
    ..Sub2p may then be displaced from Yra1p by the binding of Mex67p/Mtr2p, which participates in the export of mRNA through the nuclear pores...
  73. pmc Sus1, Cdc31, and the Sac3 CID region form a conserved interaction platform that promotes nuclear pore association and mRNA export
    Divyang Jani
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Mol Cell 33:727-37. 2009
    ..These data indicate Sac3(CID) provides a scaffold within TREX-2 to integrate interactions between protein complexes to facilitate the coupling of transcription and mRNA export during gene expression...
  74. pmc The Ct-RAE1 protein interacts with Balbiani ring RNP particles at the nuclear pore
    N Sabri
    Department of Molecular Genome Research, University of Stockholm, Sweden
    RNA 6:1597-609. 2000
    ....
  75. pmc Prediction of structural domains of TAP reveals details of its interaction with p15 and nucleoporins
    M Suyama
    EMBL, Heidelberg, Germany
    EMBO Rep 1:53-8. 2000
    ..Furthermore, the C-terminus of TAP was found to contain a ubiquitin-associated (UBA) domain. By site-directed mutagenesis we show that a conserved loop in this domain plays an essential role in mediating TAP-nucleoporin interaction...
  76. pmc The mRNA export factor Dbp5 is associated with Balbiani ring mRNP from gene to cytoplasm
    Jian Zhao
    Department of Cell and Molecular Biology, Medical Nobel Institute, Karolinska Institutet, SE 17177 Stockholm, Sweden
    EMBO J 21:1177-87. 2002
    ..Finally, the addition of the export factor Dbp5 to the growing transcript highlights the importance of the co-transcriptional loading process in determining the fate of mRNA...
  77. ncbi The nuclear export signal of splicing factor Uap56p interacts with nuclear pore-associated protein Rae1p for mRNA export in Schizosaccharomyces pombe
    Anjan G Thakurta
    Basic Research Laboratory, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 282:17507-16. 2007
    ..RNA binding and the ability to shuttle between the nucleus and cytoplasm are important features of mRNA export carriers such as HIV-Rev. Our results suggest that Uap56p could function similarly as an export carrier of mRNA in S. pombe...
  78. ncbi Cse1p functions as the nuclear export receptor for importin alpha in yeast
    M Kunzler
    Biochemie Zentrum Heidelberg, Ruprecht Karls Universitat, Germany
    FEBS Lett 433:185-90. 1998
    ..These findings suggest that Cse1p is the exportin of importin alpha in yeast...
  79. pmc Transport-related structures and processes of the nuclear pore complex studied through molecular dynamics
    Lingling Miao
    Beckman Institute for Advanced Science and Technology and Department of Physics, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Structure 17:449-59. 2009
    ..Binding to FG-repeats and a sliding motion of NTF2 induced by binding and unbinding to phenylalanines were observed when adding this transport receptor into one of the brush-like structures...
  80. ncbi Human RanBP3, a group of nuclear RanGTP binding proteins
    L Mueller
    Division for Molecular Biology of Mitosis, German Cancer Research Center, Heidelberg
    FEBS Lett 427:330-6. 1998
    ..These proteins are localized in the nucleus, preferentially bind RanGTP and may be nuclear effectors of the Ran pathway...
  81. pmc Selectivity mechanism of the nuclear pore complex characterized by single cargo tracking
    Alan R Lowe
    Department of Physics, University of California, Berkeley, California 94720, USA
    Nature 467:600-3. 2010
    ..The overall selectivity of the NPC seems to arise from the cumulative action of multiple reversible substeps and a final irreversible exit step...
  82. pmc TAP (NXF1) belongs to a multigene family of putative RNA export factors with a conserved modular architecture
    A Herold
    European Molecular Biology Laboratory, D 69117 Heidelberg, Germany
    Mol Cell Biol 20:8996-9008. 2000
    ..Both human p15 homologues bind TAP, NXF2, and NXF3. Together, our results indicate that the TAP-p15 mRNA export pathway has diversified in higher eukaryotes compared to yeast, perhaps reflecting a greater substrate complexity...
  83. ncbi Differential effects of the SR proteins 9G8, SC35, ASF/SF2, and SRp40 on the utilization of the A1 to A5 splicing sites of HIV-1 RNA
    Delphine Ropers
    Laboratoire de Maturation des ARN et Enzymologie Moleculaire, UMR CNRS 7567, Universite Henri Poincare Nancy 1, Boulevard des Aiguillettes, BP239, 54506 Vandoeuvre les Nancy, France
    J Biol Chem 279:29963-73. 2004
    ..This is the first investigation of the effects of variations of individual SR protein concentrations that is performed ex vivo on an RNA containing a complex set of splicing sites...
  84. pmc Yeast poly(A)-binding protein Pab1 shuttles between the nucleus and the cytoplasm and functions in mRNA export
    Christiane Brune
    Department of Molecular and Cell Biology, Division of Cell and Developmental Biology, University of California, Berkeley, Berkeley, CA 94720 3200, USA
    RNA 11:517-31. 2005
    ..Therefore, nuclear Pab1 may be required for efficient mRNA export and may function in the quality control of mRNA in the nucleus...
  85. pmc Kinetic analysis of translocation through nuclear pore complexes
    K Ribbeck
    ZMBH, INF 282, 69120 Heidelberg, Germany
    EMBO J 20:1320-30. 2001
    ..We propose a 'selective phase model' that could explain how NPCs function as a permeability barrier for inert molecules and yet become selectively permeable for nuclear transport receptors and receptor-cargo complexes...
  86. pmc RanBP3 enhances nuclear export of active (beta)-catenin independently of CRM1
    Jolita Hendriksen
    Department of Tumor Biology, Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands
    J Cell Biol 171:785-97. 2005
    ..We conclude that RanBP3 is a direct export enhancer for beta-catenin, independent of its role as a CRM1-associated nuclear export cofactor...
  87. pmc NAB2: a yeast nuclear polyadenylated RNA-binding protein essential for cell viability
    J T Anderson
    Department of Immunology and Medical Microbiology, College of Medicine, University of Florida, Gainesville 32610 0266
    Mol Cell Biol 13:2730-41. 1993
    ..NAB2 is located on chromosome VII within a cluster of ribonucleoprotein genes, and its expression is essential for cell growth...
  88. ncbi NXF-2, REF-1, and REF-2 affect the choice of nuclear export pathway for tra-2 mRNA in C. elegans
    Scott Kuersten
    Laboratory of Genetics, University of Wisconsin Madison, Madison, WI 53706, USA
    Mol Cell 14:599-610. 2004
    ..Furthermore, choice of proper export pathway affects tra-2 translational control. Therefore, tra-2 mRNA export is highly regulated and plays an important role in development by regulating the activity of tra-2 mRNA in the cytoplasm...
  89. ncbi Yeast centrin Cdc31 is linked to the nuclear mRNA export machinery
    Tamas Fischer
    Biochemie Zentrum der Universität Heidelberg BZH, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    Nat Cell Biol 6:840-8. 2004
    ..A previously reported cdc31 temperature-sensitive allele, which is neither defective in SPB duplication nor Kic1 kinase activation, induces mRNA export defects. Thus, Cdc31 has an unexpected link to the mRNA export machinery...
  90. ncbi TAP, the human homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus
    P Grüter
    Department of Molecular Biology, University of Geneva, Switzerland
    Mol Cell 1:649-59. 1998
    ..Thus, TAP, like its yeast homolog Mex67p, is a bona fide mRNA nuclear export mediator. TAP is the second cellular RNA binding protein shown to be directly involved in the export of its target RNA...
  91. pmc The RNA export factor Gle1p is located on the cytoplasmic fibrils of the NPC and physically interacts with the FG-nucleoporin Rip1p, the DEAD-box protein Rat8p/Dbp5p and a new protein Ymr 255p
    Y Strahm
    Microbiology Institute, CHUV, Lausanne
    EMBO J 18:5761-77. 1999
    ..We also describe a human functional homologue of Rip1p, called hCG1, which rescues Rip1p function in yeast, consistent with the evolutionary conservation of this NPC-associated protein...
  92. pmc Dbp5, a DEAD-box protein required for mRNA export, is recruited to the cytoplasmic fibrils of nuclear pore complex via a conserved interaction with CAN/Nup159p
    C Schmitt
    University of Geneva, Department of Molecular Biology, 30 Quai Ernest Ansermet, CH 1205 Geneva
    EMBO J 18:4332-47. 1999
    ..Together, our data indicate that Dbp5 is a conserved RNA-dependent ATPase which is recruited to the cytoplasmic fibrils of the NPC where it participates in the export of mRNAs out of the nucleus...
  93. ncbi Nuclear export of RNA
    Manuel S Rodriguez
    Institut Jacques Monod, UMR 7592, CNRS, Universités Paris VI and VII, 2 place Jussieu, Tour 43, 75251 Paris Cedex 05, France
    Biol Cell 96:639-55. 2004
    ....
  94. pmc Monoclonal antibodies to NTF2 inhibit nuclear protein import by preventing nuclear translocation of the GTPase Ran
    S M Steggerda
    Center for Cell Signaling, Department of Biochemistry and Molecular Genetics, and Cell and Molecular Biology Program, University of Virginia Health Sciences Center, Charlottesville, Virginia, 22908, USA
    Mol Biol Cell 11:703-19. 2000
    ..Taken together, our data show that NTF2 is an essential regulator of the Ran distribution in living cells and that NTF2-mediated Ran nuclear import is required for NLS-dependent protein import...
  95. ncbi The DEAD-box RNA helicase Dbp5 functions in translation termination
    Thomas Gross
    Institut für Molekularbiologie und Tumorforschung der Philipps Universität Marburg, Emil Mannkopff Strasse 2, 35037 Marburg, Germany
    Science 315:646-9. 2007
    ..Therefore, Dbp5 controls the eRF3-eRF1 interaction and thus eRF3-mediated downstream events...
  96. pmc NTF2 mediates nuclear import of Ran
    K Ribbeck
    Zentrum fur Molekulare Biologie der Universitat Heidelberg, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany
    EMBO J 17:6587-98. 1998
    ....
  97. ncbi Crystal structure of the spindle assembly checkpoint protein Bub3
    Nicholas A Larsen
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA
    J Mol Biol 344:885-92. 2004
    ..Comparison of the Bub3 sequence to the WD40 protein, Rae1, shows high sequence conservation along the same surfaces. Rae1 interaction with Bub1 is, therefore, likely to involve a similar mode of binding...
  98. ncbi Structural basis for the assembly of a nuclear export complex
    Yoshiyuki Matsuura
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Nature 432:872-7. 2004
    ....
  99. pmc Significant proportions of nuclear transport proteins with reduced intracellular mobilities resolved by fluorescence correlation spectroscopy
    Allison Paradise
    Department of Molecular Microbial and Structural Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030, USA
    J Mol Biol 365:50-65. 2007
    ..Variation in concentrations of freely diffusing nuclear transport intermediates among cells indicates that the nuclear transport system is sufficiently robust to function over a wide range of conditions...
  100. ncbi The Rae1-Nup98 complex prevents aneuploidy by inhibiting securin degradation
    Karthik B Jeganathan
    Department of Pediatric and Adolescent Medicine, Mayo College of Medicine, 200 First Street SW, Rochester, Minnesota 55905, USA
    Nature 438:1036-9. 2005
    ..Together, our results suggest that Rae1 and Nup98 are temporal regulators of APC(Cdh1) that maintain euploidy by preventing unscheduled degradation of securin...
  101. ncbi Identification of a tRNA-specific nuclear export receptor
    U Kutay
    Zentrum fur Molekulare Biologie, Universitat Heidelberg, Federal Republic of Germany
    Mol Cell 1:359-69. 1998
    ..RanGTP regulates the substrate-exportin-t interaction such that tRNA can be preferentially bound in the nucleus and released in the cytoplasm...

Research Grants102 found, 100 shown here

  1. Characterization Of Growth Factor-Coupled Signaling
    RICHARD CERIONE; Fiscal Year: 2006
    ..We also discovered that the nucleocytoplasmic transport proteins, a- and p-importin, as well as the Ran GTPase are key participants in this signaling pathway, ..
  2. Characterization Of Growth Factor-Coupled Signaling
    RICHARD CERIONE; Fiscal Year: 2007
    ..We also discovered that the nucleocytoplasmic transport proteins, a- and p-importin, as well as the Ran GTPase are key participants in this signaling pathway, ..
  3. Characterization Of Growth Factor-Coupled Signaling
    RICHARD CERIONE; Fiscal Year: 2009
    ..We also discovered that the nucleocytoplasmic transport proteins, a- and p-importin, as well as the Ran GTPase are key participants in this signaling pathway, ..
  4. Function of TEX11 and its Associated Proteins in Mice
    Peijing Jeremy Wang; Fiscal Year: 2010
    ..Our research will also provide insight into the molecular etiology of X-linked male infertility and birth defects (trisomy and monosomy) in humans. ..
  5. Training in Genetic, Molecular and Cellular Biology
    Michael Stallcup; Fiscal Year: 2007
    ..D. degree. ..
  6. NEURONAL DEGENERATION: MECHANISMS AND PREVENTION
    CAROL TROY; Fiscal Year: 2007
    ..4. To determine how caspases-8 and -7 are activated after SOD1 down-regulation. 5. To determine if there is activation/regulation of caspase-3 and other caspases after SOD1 down-regulation. ..
  7. Structure of Circadian Clock Complexes from Cyanobacteria by Three Dimensional EM
    Phoebe L Stewart; Fiscal Year: 2010
    ..abstract_text> ..
  8. Protein methyltransferases as transcription coactivators
    Michael Stallcup; Fiscal Year: 2003
    ..abstract_text> ..
  9. Chemical Genetic Studies of Mitosis
    RANDALL KING; Fiscal Year: 2006
    ..Understanding how exit from mitosis and adaptation is regulated will provide important insights for understanding how cancer cells respond to taxol treatment. ..
  10. CRYO-EM STRUCTURAL STUDIES OF ADENOVIRUS CELL ENTRY
    PHOEBE STEWART; Fiscal Year: 2006
    ..Increased knowledge of the Ad cell entry process may provide an opportunity to develop antivirals that block viral cell entry and will facilitate the rational design of targeted Ad vectors. ..
  11. The Nuclear Pore Complex: Interphase and Mitotic Function
    KATHARINE ULLMAN; Fiscal Year: 2005
    ..The information gathered will impact practical applications, such as optimizing expression of exogenously introduced genes as well as understanding -and ultimately controlling-growth deregulation found in cancerous cells. ..
  12. NUCLEOCYTOPLASMIC TRANSPORT OF MRNA IN YEAST
    Charles Cole; Fiscal Year: 2005
    ..Aim 3) To determine whether Dbp5 functions not only at NPCs but also in the nucleus and what it does in the nucleus. ..
  13. NEURONAL DEGENERATION: MECHANISMS AND PREVENTION
    CAROL TROY; Fiscal Year: 2005
    ..4. To determine how caspases-8 and -7 are activated after SOD1 down-regulation. 5. To determine if there is activation/regulation of caspase-3 and other caspases after SOD1 down-regulation. ..
  14. Chemical Genetic Studies of Mitosis
    RANDALL KING; Fiscal Year: 2005
    ..Understanding how exit from mitosis and adaptation is regulated will provide important insights for understanding how cancer cells respond to taxol treatment. ..
  15. Nuclear Receptor Coactivators GRIP1, CoCoA and Flil
    Michael Stallcup; Fiscal Year: 2005
    ....
  16. Chemical Genetic Studies of Mitosis
    RANDALL KING; Fiscal Year: 2004
    ..Understanding how exit from mitosis and adaptation is regulated will provide important insights for understanding how cancer cells respond to taxol treatment. ..
  17. CRYO-EM STRUCTURAL STUDIES OF ADENOVIRUS CELL ENTRY
    PHOEBE STEWART; Fiscal Year: 2004
    ..Increased knowledge of the Ad cell entry process may provide an opportunity to develop antivirals that block viral cell entry and will facilitate the rational design of targeted Ad vectors. ..
  18. Chemical Genetic Studies of Mitosis
    RANDALL KING; Fiscal Year: 2003
    ..Understanding how exit from mitosis and adaptation is regulated will provide important insights for understanding how cancer cells respond to taxol treatment. ..
  19. NUCLEOCYTOPLASMIC TRANSPORT OF MRNA IN YEAST
    Charles Cole; Fiscal Year: 2003
    ..Studies are proposed to determine the functions of Gfdl and Gfd2, and to determine how their subcellular distribution is affected by mutation of several mRNA export factors. ..
  20. Downstream Regulators B-amyloid Induced Neuronal Death
    CAROL TROY; Fiscal Year: 2003
    ..4. To determine whether the molecular components of the Abeta death pathway, identified in the preceding Aims, are altered in a mouse model of AD and/or in human AD brain. ..
  21. The Nuclear Pore Complex: Interphase and Mitotic Function
    KATHARINE ULLMAN; Fiscal Year: 2007
    ..The information gathered will impact practical applications, such as optimizing expression of exogenously introduced genes as well as understanding -and ultimately controlling-growth deregulation found in cancerous cells. ..
  22. Function of TEX11 and its Associated Proteins in Mice
    PEIJING WANG; Fiscal Year: 2007
    ..abstract_text> ..
  23. Chemical Genetic and Biochemical Studies of Mitotic Proteolysis
    RANDALL KING; Fiscal Year: 2009
    ..Together these studies will provide new chemical tools for studying the ubiquitin-proteasome pathway, and will help us understand how a critical substrate of this pathway, cyclin B1, is regulated during the cell cycle. ..
  24. CryoEM Structural Studies of DNA-PKcs and Nonhomologous End Joining Complexes
    Phoebe L Stewart; Fiscal Year: 2010
    ..Ultimately this information will be helpful in understanding and treating cancer, severe combined immune deficiency (SCID), and sensitivity to ionizing radiation (RS-SCID). ..
  25. Function of TEX11 and its Associated Proteins in Mice
    PEIJING WANG; Fiscal Year: 2009
    ..Our research will also provide insight into the molecular etiology of X-linked male infertility and birth defects (trisomy and monosomy) in humans. ..
  26. ROLE OF COREGULATORS IN STEROID HORMONE-REGULATED TRANSCRIPTION
    Michael R Stallcup; Fiscal Year: 2010
    ..The new basic knowledge obtained in this project will identify new potential targets for therapeutic intervention in these diseases. ..
  27. Regulation of chromosome synapsis in mice
    Peijing Jeremy Wang; Fiscal Year: 2010
    ..Completion of this project will provide insight into the etiology of male infertility and birth defects in humans. ..
  28. The Nuclear Pore Complex: Interphase and Mitotic Function
    KATHARINE ULLMAN; Fiscal Year: 2009
    ..Such defects in morphology and DNA content are hallmarks of aggressive cancer cells, and the research proposed here will contribute to a better understanding of how these alterations arise. ..
  29. Function of TEX11 and its Associated Proteins in Mice
    PEIJING WANG; Fiscal Year: 2009
    ..Our research will also provide insight into the molecular etiology of X-linked male infertility and birth defects (trisomy and monosomy) in humans. ..
  30. Downstream Regulators of Beta-Amyloid Induced Neuronal Death
    CAROL TROY; Fiscal Year: 2008
    ..2: To determine how caspases are regulated and activated by A[unreadable] and TFD. 3: To determine how caspase-2 regulates the induction of Bim after A[unreadable] treatment. ..
  31. Nuclear Receptor Coactivators GRIP1, CoCoA and Flil
    Michael Stallcup; Fiscal Year: 2009
    ....
  32. Downstream Regulators of Beta-Amyloid Induced Neuronal Death
    CAROL TROY; Fiscal Year: 2009
    ..2: To determine how caspase-2 is regulated and activated by A_ and TFD. 3: To determine how caspase-2 induces of Bim expression. ..
  33. Structure of Circadian Clock Complexes from Cyanobacteria by Three Dimensional EM
    PHOEBE STEWART; Fiscal Year: 2009
    ..abstract_text> ..
  34. Function of TEX11 and its Associated Proteins in Mice
    PEIJING WANG; Fiscal Year: 2008
    ..Our research will also provide insight into the molecular etiology of X-linked male infertility and birth defects (trisomy and monosomy) in humans. ..
  35. Mammalian Pre-mRNA Splicing Mechanisms
    Melissa Moore; Fiscal Year: 2007
    ..abstract_text> ..
  36. NUCLEOCYTOPLASMIC TRANSPORT OF MRNA IN YEAST
    Charles Cole; Fiscal Year: 2008
    ..Aim 3) To determine whether Dbp5 functions not only at NPCs but also in the nucleus and what it does in the nucleus. ..
  37. Chemical Genetic Studies of Mitosis
    RANDALL KING; Fiscal Year: 2007
    ..Understanding how exit from mitosis and adaptation is regulated will provide important insights for understanding how cancer cells respond to taxol treatment. ..
  38. Training in Cellular, Biochemical and Molecular Sciences
    Michael Stallcup; Fiscal Year: 2008
    ..We also have formal partnerships with BRIDGES-to-the-Ph.D. programs at local minority-serving institutions. These measures ensure that we will continue to attract a diverse group of high-quality trainees. ..
  39. Regulation of Spermiogenesis in Mice
    PEIJING WANG; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  40. The Nuclear Pore Complex: Interphase and Mitotic Function
    Katharine S Ullman; Fiscal Year: 2010
    ..Such defects in morphology and DNA content are hallmarks of aggressive cancer cells, and the research proposed here will contribute to a better understanding of how these alterations arise. ..
  41. MECHANISM OF ACTIVATION BY NUCLEAR RECEPTOR COACTIVATORS
    Michael Stallcup; Fiscal Year: 2000
    ..The long range goal is to completely define the protein components that transmit the activating signal from the activated, DNA-bound NRs to the transcription machinery. ..
  42. NUCLEOCYTOPLASMIC TRANSPORT OF MRNA IN YEAST
    Charles Cole; Fiscal Year: 2000
    ..4) The effect on nucleocytoplasmic mRNA export of mutation in a number of other yeast genes will be determined by performing the in situ poly(A)+ RNA localization assay on additional mutant yeast strains. ..
  43. REGULATION OF THE METABOLISM OF EUCARYOTIC MRNA
    Charles Cole; Fiscal Year: 1990
    ....
  44. MECHANISM OF ACTIVATION BY NUCLEAR RECEPTOR COACTIVATORS
    Michael Stallcup; Fiscal Year: 1999
    ..The long range goal is to completely define the protein components that transmit the activating signal from the activated, DNA-bound NRs to the transcription machinery. ..
  45. NUCLEOCYTOPLASMIC TRANSPORT OF MRNA IN YEAST
    Charles Cole; Fiscal Year: 1999
    ..4) The effect on nucleocytoplasmic mRNA export of mutation in a number of other yeast genes will be determined by performing the in situ poly(A)+ RNA localization assay on additional mutant yeast strains. ..
  46. REGULATION OF THE METABOLISM OF EUCARYOTIC MRNA
    Charles Cole; Fiscal Year: 1992
    ....
  47. REGULATION OF THE METABOLISM OF EUCARYOTIC MRNA
    Charles Cole; Fiscal Year: 1991
    ....
  48. GENETICS OF HORMONE BINDING TO GLUCOCORTICOID RECEPTOR
    Michael Stallcup; Fiscal Year: 1990
    ..Understanding of this phenomenon will depend upon a detailed structure-function analysis of the hormone binding pockets of these steroid receptor proteins...
  49. CRYO-EM STRUCTURAL STUDIES OF ADENOVIRUS CELL ENTRY
    PHOEBE STEWART; Fiscal Year: 2008
    ..Increased knowledge of the Ad cell entry process may provide an opportunity to develop antivirals that block viral cell entry and will facilitate the rational design of targeted Ad vectors. ..
  50. REGULATION BY A STEROID RECEPTOR HORMONE BINDING DOMAIN
    Michael Stallcup; Fiscal Year: 1999
    ....
  51. REGULATION BY A STEROID RECEPTOR HORMONE BINDING DOMAIN
    Michael Stallcup; Fiscal Year: 2001
    ....
  52. Downstream Regulators B-amyloid Induced Neuronal Death
    CAROL TROY; Fiscal Year: 2002
    ..4. To determine whether the molecular components of the Abeta death pathway, identified in the preceding Aims, are altered in a mouse model of AD and/or in human AD brain. ..
  53. NUCLEOCYTOPLASMIC TRANSPORT OF MRNA IN YEAST
    Charles Cole; Fiscal Year: 2001
    ..Studies are proposed to determine the functions of Gfdl and Gfd2, and to determine how their subcellular distribution is affected by mutation of several mRNA export factors. ..
  54. MECHANISM OF ACTIVATION BY NUCLEAR RECEPTOR COACTIVATORS
    Michael Stallcup; Fiscal Year: 2002
    ..The long range goal is to completely define the protein components that transmit the activating signal from the activated, DNA-bound NRs to the transcription machinery. ..
  55. Transcription activation by nuclear coactivators
    Michael Stallcup; Fiscal Year: 2002
    ..abstract_text> ..
  56. Chemical Genetic Studies of Mitosis
    RANDALL KING; Fiscal Year: 2002
    ..Understanding how exit from mitosis and adaptation is regulated will provide important insights for understanding how cancer cells respond to taxol treatment. ..
  57. HERPES SIMPLEX VIRUS GENE REGULATION
    ROZANNE SANDRI GOLDIN; Fiscal Year: 1993
    ..Few factors which affect the efficiency and specificity of RNA processing have been Identified. The possible participation of ICP27 in both splicing and polyadenylation could uncover factors which link these complex processes...
  58. HERPES SIMPLEX VIRUS GENE REGULATION
    ROZANNE SANDRI GOLDIN; Fiscal Year: 1990
    ..Mutant proteins will be isolated and analyzed to determine which functions or activities are affected. In this way, a functional map of each protein can be derived...
  59. Acquisition of a Liquid Helium Cryo-Electron Microscope
    PHOEBE STEWART; Fiscal Year: 2003
    ..The financial plan for the long term operation of the microscope includes a significant additional commitment from UCLA for microscope operating expenses. ..
  60. GENETICS OF HORMONE BINDING TO GLUCOCORTICOID RECEPTOR
    Michael Stallcup; Fiscal Year: 1993
    ..Understanding of this phenomenon will depend upon a detailed structure-function analysis of the hormone binding pockets of these steroid receptor proteins...
  61. Nuclear Receptor Coactivators GRIP1, CoCoA and Flil
    Michael Stallcup; Fiscal Year: 2008
    ....
  62. HERPES SIMPLEX VIRUS GENE REGULATION
    ROZANNE SANDRI GOLDIN; Fiscal Year: 1992
    ..Few factors which affect the efficiency and specificity of RNA processing have been Identified. The possible participation of ICP27 in both splicing and polyadenylation could uncover factors which link these complex processes...
  63. NUCLEOCYTOPLASMIC TRANSPORT OF MRNA IN YEAST
    Charles Cole; Fiscal Year: 2002
    ..Studies are proposed to determine the functions of Gfdl and Gfd2, and to determine how their subcellular distribution is affected by mutation of several mRNA export factors. ..
  64. Mechanism of p160 nuclear coactivators
    Michael Stallcup; Fiscal Year: 2003
    ..abstract_text> ..
  65. MOLECULAR MECHANISMS OF NUCLEAR EXPORT
    KATHARINE ULLMAN; Fiscal Year: 2002
    ..Through genetic and biochemical interaction analyses, Nup153 will be used to construct a bigger picture of key connections made between export machinery components. ..
  66. Domain structure and interactions of HSV-1 ICP27
    ROZANNE SANDRI GOLDIN; Fiscal Year: 2007
    ..abstract_text> ..
  67. Mechanism of p160 nuclear coactivators
    Michael Stallcup; Fiscal Year: 2004
    ..abstract_text> ..
  68. CRYO-EM STRUCTURAL STUDIES OF ADENOVIRUS CELL ENTRY
    PHOEBE STEWART; Fiscal Year: 2009
    ..Understanding the molecular principles of this natural defense mechanism could lead to novel strategies for antiviral drug design. ..
  69. MOLECULAR MECHANISMS OF NUCLEAR EXPORT
    KATHARINE ULLMAN; Fiscal Year: 2004
    ..Through genetic and biochemical interaction analyses, Nup153 will be used to construct a bigger picture of key connections made between export machinery components. ..
  70. Chemical Genetic and Biochemical Studies of Mitotic Proteolysis
    RANDALL KING; Fiscal Year: 2008
    ..Together these studies will provide new chemical tools for studying the ubiquitin-proteasome pathway, and will help us understand how a critical substrate of this pathway, cyclin B1, is regulated during the cell cycle. ..
  71. NUCLEOCYTOPLASMIC TRANSPORT OF MRNA IN YEAST
    Charles Cole; Fiscal Year: 2004
    ..Studies are proposed to determine the functions of Gfdl and Gfd2, and to determine how their subcellular distribution is affected by mutation of several mRNA export factors. ..
  72. MECHANISM OF ACTIVATION BY NUCLEAR RECEPTOR COACTIVATORS
    Michael Stallcup; Fiscal Year: 2001
    ..The long range goal is to completely define the protein components that transmit the activating signal from the activated, DNA-bound NRs to the transcription machinery. ..
  73. Domain structure and interactions of HSV-1 ICP27
    ROZANNE SANDRI GOLDIN; Fiscal Year: 2006
    ..abstract_text> ..
  74. Influence of glycosylation on nuclear pore function
    KENNETH BELANGER; Fiscal Year: 2005
    ....
  75. Downstream Regulators B-amyloid Induced Neuronal Death
    CAROL TROY; Fiscal Year: 2001
    ..4. To determine whether the molecular components of the Abeta death pathway, identified in the preceding Aims, are altered in a mouse model of AD and/or in human AD brain. ..
  76. HERPES SIMPLEX VIRUS GENE REGULATION
    ROZANNE SANDRI GOLDIN; Fiscal Year: 2007
    ..abstract_text> ..
  77. Regulation of Spermiogenesis in Mice
    PEIJING WANG; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  78. HERPES SIMPLEX VIRUS GENE REGULATION
    ROZANNE SANDRI GOLDIN; Fiscal Year: 2001
    ....
  79. HERPES SIMPLEX VIRUS GENE REGULATION
    ROZANNE SANDRI GOLDIN; Fiscal Year: 2000
    ....
  80. NUCLEOCYTOPLASMIC TRANSPORT OF MRNA IN YEAST
    Charles Cole; Fiscal Year: 2007
    ..Aim 3) To determine whether Dbp5 functions not only at NPCs but also in the nucleus and what it does in the nucleus. ..
  81. Nuclear Receptor Coactivators GRIP1, CoCoA and Flil
    Michael Stallcup; Fiscal Year: 2007
    ....
  82. REGULATION BY A STEROID RECEPTOR HORMONE BINDING DOMAIN
    Michael Stallcup; Fiscal Year: 2000
    ....
  83. Domain structure and interactions of HSV-1 ICP27
    ROZANNE SANDRI GOLDIN; Fiscal Year: 2004
    ..abstract_text> ..
  84. Domain structure and interactions of HSV-1 ICP27
    ROZANNE SANDRI GOLDIN; Fiscal Year: 2005
    ..abstract_text> ..
  85. The Nuclear Pore Complex: Interphase and Mitotic Function
    KATHARINE ULLMAN; Fiscal Year: 2005
    ..The information gathered will impact practical applications, such as optimizing expression of exogenously introduced genes as well as understanding -and ultimately controlling-growth deregulation found in cancerous cells. ..
  86. Protein methyltransferases as transcription coactivators
    Michael Stallcup; Fiscal Year: 2007
    ..abstract_text> ..
  87. NEURONAL DEGENERATION: MECHANISMS AND PREVENTION
    CAROL TROY; Fiscal Year: 2006
    ..4. To determine how caspases-8 and -7 are activated after SOD1 down-regulation. 5. To determine if there is activation/regulation of caspase-3 and other caspases after SOD1 down-regulation. ..
  88. Domain structure and interactions of HSV-1 ICP27
    ROZANNE SANDRI GOLDIN; Fiscal Year: 2005
    ..abstract_text> ..