n type calcium channels

Summary

Summary: CALCIUM CHANNELS that are concentrated in neural tissue. Omega toxins inhibit the actions of these channels by altering their voltage dependence.

Top Publications

  1. ncbi Distribution of voltage-dependent calcium channel beta subunits in the hippocampus of patients with temporal lobe epilepsy
    A A Lie
    Department of Epileptology, University of Bonn Medical Center, Germany
    Neuroscience 93:449-56. 1999
  2. pmc Membrane depolarization inhibits spiral ganglion neurite growth via activation of multiple types of voltage sensitive calcium channels and calpain
    Pamela C Roehm
    Department of Otolaryngology Head and Neck Surgery, University of Iowa, Iowa City, IA 52242, USA
    Mol Cell Neurosci 37:376-87. 2008
  3. ncbi Interaction via a key tryptophan in the I-II linker of N-type calcium channels is required for beta1 but not for palmitoylated beta2, implicating an additional binding site in the regulation of channel voltage-dependent properties
    Jérôme Leroy
    Department of Pharmacology, University College London, London WC1E 6BT, United Kingdom
    J Neurosci 25:6984-96. 2005
  4. ncbi Transfer of beta subunit regulation from high to low voltage-gated Ca2+ channels
    Juan Manuel Arias
    Department of Pharmacology, University of Virginia, Charlottesville, VA 22908, USA
    FEBS Lett 579:3907-12. 2005
  5. ncbi Endocannabinoids inhibit transmission at granule cell to Purkinje cell synapses by modulating three types of presynaptic calcium channels
    Solange P Brown
    Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Neurosci 24:5623-31. 2004
  6. ncbi Unified mechanisms of Ca2+ regulation across the Ca2+ channel family
    Haoya Liang
    Ca2 Signals Laboratory, Departments of Biomedical Engineering and Neuroscience, The Johns Hopkins University School of Medicine, 720 Rutland Avenue, Baltimore, MD 21205, USA
    Neuron 39:951-60. 2003
  7. ncbi Differential role of N-type calcium channel splice isoforms in pain
    Christophe Altier
    Hotchkiss Brain Institute, Department of Physiology and Biophysics, University of Calgary, Calgary, Alberta, Canada T2N 1N4
    J Neurosci 27:6363-73. 2007
  8. ncbi Cell-specific alternative splicing increases calcium channel current density in the pain pathway
    Thomas J Bell
    Department of Neuroscience, Brown University, Providence, RI 02912, USA
    Neuron 41:127-38. 2004
  9. pmc Alternative splicing controls G protein-dependent inhibition of N-type calcium channels in nociceptors
    Jesica Raingo
    Department of Neuroscience, Brown University, Sidney E Frank Hall for Life Sciences, 185 Meeting Street, Providence, Rhode Island 02912, USA
    Nat Neurosci 10:285-92. 2007
  10. pmc Molecular determinants of Ca(2+)/calmodulin-dependent regulation of Ca(v)2.1 channels
    Amy Lee
    Department of Pharmacology, University of Washington School of Medicine, Seattle, WA 98195 7280, USA
    Proc Natl Acad Sci U S A 100:16059-64. 2003

Research Grants

  1. PRESYNAPTIC RECEPTORS ON EMBRYONIC SENSORY NEURONS
    Kathleen Dunlap; Fiscal Year: 2007
  2. Nitrergic Neuro-smooth Muscle Transmission in the Gut
    Raj K Goyal; Fiscal Year: 2013
  3. Regulation of (Ca2+)i oscillation and osteoclast differentiation by RGS12
    Shuying Yang; Fiscal Year: 2010
  4. N-type Calcium Channels in Nociceptive Neurons
    Diane Lipscombe; Fiscal Year: 2013
  5. Regulation and function of hippocampal excrescences
    Daniel T Pak; Fiscal Year: 2011
  6. PATCH CLAMP STUDY OF GASTROINTESTINAL NEURONS
    JACKIE WOOD; Fiscal Year: 1999
  7. Farnesol Analogues as Novel Treatment of Alcoholism
    Jean Baptiste Roullet; Fiscal Year: 2005
  8. STUDIES OF N-TYPE CALCIUM CHANNELS
    Aaron Fox; Fiscal Year: 2003
  9. N TYPE CA CHANNELS IN GROWTH CONES
    Maureen McEnery; Fiscal Year: 2000
  10. CALCIUM CHANNELS IN SYMPATHETIC NEURONS
    KEITH ELMSLIE; Fiscal Year: 2000

Detail Information

Publications196 found, 100 shown here

  1. ncbi Distribution of voltage-dependent calcium channel beta subunits in the hippocampus of patients with temporal lobe epilepsy
    A A Lie
    Department of Epileptology, University of Bonn Medical Center, Germany
    Neuroscience 93:449-56. 1999
    ..Altered expression of these beta subunits may lead to increased currents carried by voltage-dependent calcium channels and to enhanced synaptic excitability...
  2. pmc Membrane depolarization inhibits spiral ganglion neurite growth via activation of multiple types of voltage sensitive calcium channels and calpain
    Pamela C Roehm
    Department of Otolaryngology Head and Neck Surgery, University of Iowa, Iowa City, IA 52242, USA
    Mol Cell Neurosci 37:376-87. 2008
    ....
  3. ncbi Interaction via a key tryptophan in the I-II linker of N-type calcium channels is required for beta1 but not for palmitoylated beta2, implicating an additional binding site in the regulation of channel voltage-dependent properties
    Jérôme Leroy
    Department of Pharmacology, University College London, London WC1E 6BT, United Kingdom
    J Neurosci 25:6984-96. 2005
    ....
  4. ncbi Transfer of beta subunit regulation from high to low voltage-gated Ca2+ channels
    Juan Manuel Arias
    Department of Pharmacology, University of Virginia, Charlottesville, VA 22908, USA
    FEBS Lett 579:3907-12. 2005
    ..Taken together, these results support a direct coupling model where beta subunits alter movements in IS6 that occur as the channel transits between closed, open, and inactivated states...
  5. ncbi Endocannabinoids inhibit transmission at granule cell to Purkinje cell synapses by modulating three types of presynaptic calcium channels
    Solange P Brown
    Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Neurosci 24:5623-31. 2004
    ....
  6. ncbi Unified mechanisms of Ca2+ regulation across the Ca2+ channel family
    Haoya Liang
    Ca2 Signals Laboratory, Departments of Biomedical Engineering and Neuroscience, The Johns Hopkins University School of Medicine, 720 Rutland Avenue, Baltimore, MD 21205, USA
    Neuron 39:951-60. 2003
    ....
  7. ncbi Differential role of N-type calcium channel splice isoforms in pain
    Christophe Altier
    Hotchkiss Brain Institute, Department of Physiology and Biophysics, University of Calgary, Calgary, Alberta, Canada T2N 1N4
    J Neurosci 27:6363-73. 2007
    ..In contrast, both N-type channel isoforms (e37a- and e37b-containing) contribute to tactile neuropathic allodynia. Hence, exon 37a acts as a molecular switch that tailors the channels toward specific roles in pain...
  8. ncbi Cell-specific alternative splicing increases calcium channel current density in the pain pathway
    Thomas J Bell
    Department of Neuroscience, Brown University, Providence, RI 02912, USA
    Neuron 41:127-38. 2004
    ..8. Cell-specific inclusion of exon 37a correlates closely with significantly larger N-type currents in nociceptive neurons. This unique splice isoform of the N-type channel could represent a novel target for pain management...
  9. pmc Alternative splicing controls G protein-dependent inhibition of N-type calcium channels in nociceptors
    Jesica Raingo
    Department of Neuroscience, Brown University, Sidney E Frank Hall for Life Sciences, 185 Meeting Street, Providence, Rhode Island 02912, USA
    Nat Neurosci 10:285-92. 2007
    ..Our data define the molecular origins of voltage-independent inhibition of N-type channels in the pain pathway...
  10. pmc Molecular determinants of Ca(2+)/calmodulin-dependent regulation of Ca(v)2.1 channels
    Amy Lee
    Department of Pharmacology, University of Washington School of Medicine, Seattle, WA 98195 7280, USA
    Proc Natl Acad Sci U S A 100:16059-64. 2003
    ..This multifaceted mechanism allows positive regulation of Cav2.1 in response to local Ca2+ increases (CDF) and negative regulation during more global Ca2+ increases (CDI)...
  11. pmc Familial hemiplegic migraine mutations increase Ca(2+) influx through single human CaV2.1 channels and decrease maximal CaV2.1 current density in neurons
    Angelita Tottene
    Department of Biomedical Sciences, University of Padova, 35121 Padova, Italy
    Proc Natl Acad Sci U S A 99:13284-9. 2002
    ..1 current density in neurons. We discuss the relevance of these findings for the pathogenesis of migraine with aura...
  12. pmc Elementary mechanisms producing facilitation of Cav2.1 (P/Q-type) channels
    Dipayan Chaudhuri
    Department of Neuroscience, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    J Gen Physiol 129:385-401. 2007
    ..This enhanced-opening mechanism suggests that the CDF evoked during action-potential trains would produce not only larger, but longer-lasting Ca(2+) responses, an outcome with potential ramifications for short-term synaptic plasticity...
  13. pmc Alternative splicing in the C-terminus of CaV2.2 controls expression and gating of N-type calcium channels
    Andrew J Castiglioni
    Department of Neuroscience, Box 1953, Brown University, Providence, RI 02912, USA
    J Physiol 576:119-34. 2006
    ..Our studies of the e37a/e37b splice site reveal a multifunctional domain in the C-terminus of Ca(V)2.2 that regulates the overall activity of N-type calcium channels in nociceptors...
  14. pmc Localization of the activation gate of a voltage-gated Ca2+ channel
    Cheng Xie
    Department of Biological Sciences, Columbia University, New York, NY 10027, USA
    J Gen Physiol 126:205-12. 2005
    ..Our results suggest that the S6 helices of the alpha1 subunit of VGCCs undergo conformation changes during gating and the activation gate is located at the intracellular end of the pore...
  15. pmc Differential facilitation of N- and P/Q-type calcium channels during trains of action potential-like waveforms
    Kevin P M Currie
    Department of Neurobiology, Pharmacology and Physiology, University of Chicago, 947 E 58th Street, MC 0926, Chicago, IL 60637, USA
    J Physiol 539:419-31. 2002
    ..Since the ratio of N-type to P/Q-type Ca(2+) channels varies widely between synapses, differential facilitation may contribute to the fine tuning of synaptic transmission, thereby increasing the computational repertoire of neurons...
  16. pmc Capacitance measurements of exocytosis in mouse pancreatic alpha-, beta- and delta-cells within intact islets of Langerhans
    Sven Göpel
    Department of Physiological Sciences, BMC F11, SE 221 84 Lund, Sweden
    J Physiol 556:711-26. 2004
    ....
  17. ncbi Biochemical and anatomical evidence for specialized voltage-dependent calcium channel gamma isoform expression in the epileptic and ataxic mouse, stargazer
    A H Sharp
    Department of Physiology and Biophysics, Case Western Reserve University, School of Medicine, Cleveland, OH 44106 4970, USA
    Neuroscience 105:599-617. 2001
    ....
  18. pmc The novel product of a five-exon stargazin-related gene abolishes Ca(V)2.2 calcium channel expression
    Fraser J Moss
    Department of Pharmacology, University College London, Gower Street, London WC1E 6BT, UK
    EMBO J 21:1514-23. 2002
    ....
  19. ncbi Long splice variant N type calcium channels are clustered at presynaptic transmitter release sites without modular adaptor proteins
    R Khanna
    Cellular and Molecular Biology Division, Toronto Western Research Institute, University Health Network, 399 Bathurst Street, MP14 320, Toronto, Ontario, Canada M5T 2S8
    Neuroscience 138:1115-25. 2006
    ..2 argues against the idea that these modular adaptor proteins anchor CaV2.2 at presynaptic nerve terminals...
  20. ncbi Preassociation of calmodulin with voltage-gated Ca(2+) channels revealed by FRET in single living cells
    M G Erickson
    Department of Biomedical Engineering, Calcium Signals Laboratory, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Neuron 31:973-85. 2001
    ..These results now definitively demonstrate channel-CaM preassociation in resting cells and underscore the potential of three-cube FRET for probing protein-protein interactions...
  21. pmc Decoding of synaptic voltage waveforms by specific classes of recombinant high-threshold Ca(2+) channels
    Zhi Liu
    Kinsmen Laboratory and Brain Research Centre, University of British Columbia, Vancouver, BC, Canada
    J Physiol 553:473-88. 2003
    ..We propose that in response to complex synaptic voltage waveforms P/Q- and N-type channels can undergo selective voltage-dependent inactivation leading to a Ca(2+) current mediated predominantly by L-type channels...
  22. ncbi Functional consequences of P/Q-type Ca2+ channel Cav2.1 missense mutations associated with episodic ataxia type 2 and progressive ataxia
    Edwin Wappl
    Institut fur Biochemische Pharmakologie, Abteilung Pharmakologie und Toxikologie, Institut fur Pharmazie, Universitat Innsbruck, Peter Mayrstrasse 1, A 6020 Innsbruck, Austria
    J Biol Chem 277:6960-6. 2002
    ..In contrast to other EA-2 mutations, AY1593/1594D and G293R form at least partially functional channels...
  23. ncbi Effects of ablation of N- and R-type Ca(2+) channels on pain transmission
    Hironao Saegusa
    Department of Pharmacology and Neurobiology, Graduate School of Medicine, Tokyo Medical and Dental University, and CREST, Japan Science and Technology Corporation, Tokyo, Japan
    Neurosci Res 43:1-7. 2002
    ..3-/- mice, was also observed in the Ca(v)2.2-/- mice. Therefore, it is suggested that these mutant mice could provide novel models to delineate the nociceptive and antinociceptive mechanisms...
  24. ncbi Nitric oxide augments voltage-gated P/Q-type Ca(2+) channels constituting a putative positive feedback loop
    Jianguo Chen
    Department of Physiology and Biophysics, The University of Iowa, Iowa City, IA, USA
    Free Radic Biol Med 32:638-49. 2002
    ....
  25. ncbi Specific kinetic alterations of human CaV2.1 calcium channels produced by mutation S218L causing familial hemiplegic migraine and delayed cerebral edema and coma after minor head trauma
    Angelita Tottene
    Department of Biomedical Sciences, University of Padova, CNR Institute of Neuroscience, Viale G Colombo 3, 35121 Padova, Italy
    J Biol Chem 280:17678-86. 2005
    ....
  26. ncbi A destructive interaction mechanism accounts for dominant-negative effects of misfolded mutants of voltage-gated calcium channels
    Alexandre Mezghrani
    Centre National de la Recherche Scientifique, Unite Mixte de Recherche 5203, Institut de Genomique Fonctionnelle, Institut National de la Sante et de la Recherche Medicale, 34094 Montpellier, France
    J Neurosci 28:4501-11. 2008
    ..We conclude that this destructive interaction mechanism promoted by Ca(v) mutants is likely to occur in EA2 and in other inherited dominant channelopathies...
  27. ncbi Single tottering mutations responsible for the neuropathic phenotype of the P-type calcium channel
    M Wakamori
    Department of Information Physiology, National Institute for Physiological Sciences, Okazaki, Aichi 444 8585, Japan
    J Biol Chem 273:34857-67. 1998
    ....
  28. ncbi Direct G protein modulation of Cav2 calcium channels
    H William Tedford
    Hotchkiss Brain Institute, Department of Physiology and Biophysics, University of Calgary, Calgary, Canada
    Pharmacol Rev 58:837-62. 2006
    ..Twenty-five years after this mode of physiological regulation was first described, we review the investigations that have led to our current understanding of its molecular mechanisms...
  29. ncbi Alternative splicing of the Ca2+ channel beta4 subunit confers specificity for gabapentin inhibition of Cav2.1 trafficking
    Patrice M Mich
    Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14850, USA
    Mol Pharmacol 74:904-12. 2008
    ..This mechanism may help to explain why gabapentin is both effective and selective in the treatment of neuropathic pain states that involve up-regulation of alpha2delta subunits...
  30. ncbi Ca(V)2.1 P/Q-type calcium channel alternative splicing affects the functional impact of familial hemiplegic migraine mutations: implications for calcium channelopathies
    Paul J Adams
    Michael Smith Laboratories, University of British Columbia, Vancouver, BC, CA
    Channels (Austin) 3:110-21. 2009
    ..Our findings provide important insight concerning the role of calcium channel alternatively spliced variants and the molecular pathophysiology of FHM-1 and potentially of other calcium channelopathies...
  31. pmc Calcium channel beta subunit promotes voltage-dependent modulation of alpha 1 B by G beta gamma
    A Meir
    Department of Pharmacology, University College London, London WC1E 6BT, United Kingdom
    Biophys J 79:731-46. 2000
    ..These results suggest that the VDCC beta subunit must be present for G beta gamma to induce voltage-dependent modulation of N-type calcium channels...
  32. ncbi Mutations in high-voltage-activated calcium channel genes stimulate low-voltage-activated currents in mouse thalamic relay neurons
    Yi Zhang
    Developmental Neurogenetics Laboratory, Department of Neurology, Baylor College of Medicine, Houston, Texas 77030, USA
    J Neurosci 22:6362-71. 2002
    ..These alterations increase the probability for abnormal thalamocortical synchronization and absence epilepsy in tg, lh, and stg mice...
  33. ncbi G protein-gated inhibitory module of N-type (ca(v)2.2) ca2+ channels
    Heather L Agler
    Department of Biomedical Engineering and Ca2 Signals Laboratory, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Neuron 46:891-904. 2005
    ..Thus, an NT module, acting via interactions with the I-II loop, appears fundamental to such modulation...
  34. pmc Modal gating of human CaV2.1 (P/Q-type) calcium channels: II. the b mode and reversible uncoupling of inactivation
    Tommaso Fellin
    Dept of Biomedical Sciences, University of Padova, Viale G Colombo, 3 35121 Padova, Italy
    J Gen Physiol 124:463-74. 2004
    ..1 channels could be a potent and versatile mechanism for the modulation of synaptic strength and plasticity as well as of neuronal excitability and other postsynaptic Ca2+-dependent processes...
  35. pmc Modal gating of human CaV2.1 (P/Q-type) calcium channels: I. The slow and the fast gating modes and their modulation by beta subunits
    Siro Luvisetto
    Dept of Biomedical Sciences, University of Padova, Viale G Colombo 3, 35121 Padova, Italy
    J Gen Physiol 124:445-61. 2004
    ....
  36. ncbi The interaction between the I-II loop and the III-IV loop of Cav2.1 contributes to voltage-dependent inactivation in a beta -dependent manner
    Sandrine Geib
    INSERM Unité 464, Laboratoire de Neurobiologie des Canaux Ioniques, Faculte de Medecine Nord, Boulevard Pierre Dramard, 13916 Marseille Cedex 20, France
    J Biol Chem 277:10003-13. 2002
    ..These data provide novel insights into the mechanisms whereby the beta subunit, the I-II loop, and the III-IV loop altogether can contribute to regulate inactivation in high voltage-activated calcium channels...
  37. ncbi Dominant-negative synthesis suppression of voltage-gated calcium channel Cav2.2 induced by truncated constructs
    A Raghib
    Department of Pharmacology, University College London, London WC1E6BT, United Kingdom
    J Neurosci 21:8495-504. 2001
    ..Our results indicate that the mechanism of suppression of Ca(v)2.2 by truncated constructs containing domain I involves inhibition of channel synthesis, which may represent a role of endogenously expressed truncated Ca(v) isoforms...
  38. ncbi Presynaptic Ca2+ channels and neurotransmitter release at the terminal of a mouse cortical neuron
    J Qian
    Department of Neurology, Baylor College of Medicine, Houston, Texas 77030, USA
    J Neurosci 21:3721-8. 2001
    ....
  39. ncbi Spinocerebellar ataxia type 6: channelopathy or glutamine repeat disorder?
    M Frontali
    Istituto di Neurobiologia e Medicina Molecolare, Consiglio Nazionale delle Ricerche, Rome, Italy
    Brain Res Bull 56:227-31. 2001
    ..In conclusion, available data seem to suggest that SCA6 is more likely belonging to channelopathies than to polyglutamine disorders...
  40. pmc Functional roles of gamma2, gamma3 and gamma4, three new Ca2+ channel subunits, in P/Q-type Ca2+ channel expressed in Xenopus oocytes
    M Rousset
    CRBM, CNRS UPR 1086, UFR 24, 1919 Route de Mende, 34293 Montpellier Cedex 05, France
    J Physiol 532:583-93. 2001
    ..Our data support a role for the [gamma] subunit as a brain Ca2+ channel modulatory subunit and suggest that [beta] and [gamma] subunits are involved in a switch between two regulatory modes of the P/Q-type channel inactivation...
  41. pmc Suppression of inflammatory and neuropathic pain symptoms in mice lacking the N-type Ca2+ channel
    H Saegusa
    Department of Pharmacology and Neurobiology, Graduate School of Medicine, Tokyo Medical and Dental University and CREST, Japan Science and Technology Corporation, 1 5 45 Yushima, Bunkyo ku, Tokyo 113 8519, Japan
    EMBO J 20:2349-56. 2001
    ..This finding clearly demonstrates that the N-type VDCC is essential for development of neuropathic pain and, therefore, controlling the activity of this channel can be of great importance for the management of neuropathic pain...
  42. pmc RGS2 blocks slow muscarinic inhibition of N-type Ca(2+) channels reconstituted in a human cell line
    K Melliti
    Department of Biology, Utah State University, Logan 84322-5305, USA
    J Physiol 532:337-47. 2001
    ..These results identify RGS2 as a potential physiological regulator of the slow muscarinic pathway...
  43. ncbi Phosphatidylinositol [correction] 4,5-bisphosphate signals underlie receptor-specific Gq/11-mediated modulation of N-type Ca2+ channels
    Nikita Gamper
    Department of Physiology, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229, USA
    J Neurosci 24:10980-92. 2004
    ..We suggest that differential use of PIP2 signals underlies specificity of Gq/11-coupled receptor actions on the channels..
  44. ncbi Arachidonic acid both inhibits and enhances whole cell calcium currents in rat sympathetic neurons
    L Liu
    Program in Neuroscience, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    Am J Physiol Cell Physiol 280:C1293-305. 2001
    ..AA also enhanced the rate of N-type current activation. These findings indicate that AA causes multiple changes in sympathetic Ca(2+) currents...
  45. ncbi Physiological patterns of electrical stimulation can induce neuronal gene expression by activating N-type calcium channels
    T A Brosenitsch
    Department of Neurosciences, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106, USA
    J Neurosci 21:2571-9. 2001
    ..Moreover, our data show that phasic and chronic depolarizing stimuli act through distinct mechanisms to induce neuronal gene expression...
  46. ncbi Novel Cav2.1 splice variants isolated from Purkinje cells do not generate P-type Ca2+ current
    Taiji Tsunemi
    Department of Pharmacology and Neurobiology, Graduate School of Medicine, Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo ku, Tokyo 113 8519, Japan
    J Biol Chem 277:7214-21. 2002
    ..These results suggest that P-type-specific splice variants may exist but that post-translational processing or modification by uncharacterized interacting proteins is also required for generating the P-type current...
  47. ncbi Increased glucose tolerance in N-type Ca2+ channel alpha(1B)-subunit gene-deficient mice
    Eiki Takahashi
    Tsukuba Research Laboratory, Eisai Co Ltd, Tsukuba, Ibaraki 300 2635, Japan
    Int J Mol Med 15:937-44. 2005
    ..Thus, N-type Ca2+ channel blockers might be candidate anti-diabetic/anti-obesity agents...
  48. ncbi Phospholipid metabolism is required for M1 muscarinic inhibition of N-type calcium current in sympathetic neurons
    Liwang Liu
    Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    Eur Biophys J 33:255-64. 2004
    ..In contrast to AA, applying LPA did not inhibit whole cell currents. Taken together, these findings suggest that the slow pathway requires M1Rs, Gqalpha, PLC, PIP2, PLA2, and AA for N-current inhibition...
  49. ncbi The calcium channel ligand FPL 64176 enhances L-type but inhibits N-type neuronal calcium currents
    Liwang Liu
    Program in Neuroscience, University of Massachusetts Medical Center, 55 Lake Ave North, Worcester, MA 01655, USA
    Neuropharmacology 45:281-92. 2003
    ....
  50. pmc Pharmacological discrimination between muscarinic receptor signal transduction cascades with bethanechol chloride
    Liwang Liu
    Program in Neuroscience, Program in Cellular and Molecular Physiology, Department of Physiology, University of Massachusetts Medical Center, 55 Lake Ave North, Worcester, MA 01655, U S A
    Br J Pharmacol 138:1259-70. 2003
    ..5. These results indicate that BeCh may be an effective tool for selectively activating M(2) receptor stimulation of the membrane-delimited pathway...
  51. pmc Origin of the voltage dependence of G-protein regulation of P/Q-type Ca2+ channels
    Yun Zhang
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    J Neurosci 28:14176-88. 2008
    ....
  52. ncbi Direct interaction and functional coupling between metabotropic glutamate receptor subtype 1 and voltage-sensitive Cav2.1 Ca2+ channel
    Jun Kitano
    Department of Biological Sciences, Faculty of Medicine, Kyoto University, Japan
    J Biol Chem 278:25101-8. 2003
    ..1 were observed at dendrites but not soma of cultured Purkinje neurons. These data suggest that the assembly of mGluR1 and Cav2.1 provides the mechanism that ensures spatiotemporal regulation of [Ca2+]i in glutamatergic neurotransmission...
  53. ncbi Cell-type-specific alternative splicing in spinocerebellar ataxia type 6
    Taiji Tsunemi
    Department of Neurology and Neurological Science, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan
    Neurosci Lett 447:78-81. 2008
    ..Our results suggest that toxic isoform mRNA is increased in a Purkinje cell-specific manner, which may result in SCA6-associated selective neurodegeneration...
  54. ncbi Several structural domains contribute to the regulation of N-type calcium channel inactivation by the beta 3 subunit
    Stephanie C Stotz
    Department of Physiology and Biophysics Cellular and Molecular Neurobiology Research Group, University of Calgary, Calgary, Alberta T2N 4N1, Canada
    J Biol Chem 279:3793-800. 2004
    ..2 did not alter the regulation of the channel by wild type and chimeric beta subunits. Hence, the molecular underpinnings of beta subunit regulation of voltage-gated calcium channels appear to vary with calcium channel subtype...
  55. pmc Arachidonic acid mediates muscarinic inhibition and enhancement of N-type Ca2+ current in sympathetic neurons
    Liwang Liu
    Program in Neuroscience, Department of Physiology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester 01655, USA
    Proc Natl Acad Sci U S A 100:295-300. 2003
    ..Our finding that AA participates in the slow pathway strongly suggests that it may be the previously unknown diffusible second messenger...
  56. ncbi L- and N-current but not M-current inhibition by M1 muscarinic receptors requires DAG lipase activity
    Liwang Liu
    Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Cell Physiol 216:91-100. 2008
    ..These results extend our previous findings that the signaling pathway mediating L- and N-current inhibition diverges from the pathway initiating M-current inhibition...
  57. ncbi Whole-cell and single-channel analysis of P-type calcium currents in cerebellar Purkinje cells of leaner mutant mice
    L S Dove
    Department of Medical Pharmacology and Toxicology, College of Medicine, Texas A and M University Health Science Center, College Station, Texas 77843 1114, USA
    J Neurosci 18:7687-99. 1998
    ..These results suggest that the tgla mutation can alter native P-type calcium channels at the single-channel level and that these alterations may contribute to the neuropathology of the leaner phenotype...
  58. ncbi The expression of voltage-dependent calcium channel beta subunits in human hippocampus
    N C Day
    MRC Neurochemical Pathology Unit, University of Newcastle upon Tyne, Newcastle General Hospital, Westgate Road, Newcastle upon Tyne NE4 6BE, UK
    Brain Res Mol Brain Res 60:259-69. 1998
    ....
  59. ncbi Neurotransmitter modulation of neuronal calcium channels
    Keith S Elmslie
    Department of Physiology, Tulane University Health Science Center, New Orleans, Louisiana 70112, USA
    J Bioenerg Biomembr 35:477-89. 2003
    ....
  60. ncbi Endocannabinoids modulate N-type calcium channels and G-protein-coupled inwardly rectifying potassium channels via CB1 cannabinoid receptors heterologously expressed in mammalian neurons
    Juan Guo
    Laboratory of Molecular Physiology, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, MD 20892 8815, USA
    Mol Pharmacol 65:665-74. 2004
    ..These results suggest a mechanism by which endocannibinoids might influence presynaptic function...
  61. ncbi Reduced voltage sensitivity of activation of P/Q-type Ca2+ channels is associated with the ataxic mouse mutation rolling Nagoya (tg(rol))
    Y Mori
    Department of Information Physiology, National Institute for Physiological Sciences, The Graduate University for Advanced Studies, Okazaki, Aichi, Japan
    J Neurosci 20:5654-62. 2000
    ....
  62. ncbi Alternative splicing in the voltage-sensing region of N-Type CaV2.2 channels modulates channel kinetics
    Yingxin Lin
    Deprtment of Neuroscience, Brown University, 192 Thayer St, Providence, RI 02912, USA
    J Neurophysiol 92:2820-30. 2004
    ..Our results suggest that alternative splicing in the S3-S4 linker influences the kinetics but not the voltage dependence of N-type channel gating...
  63. ncbi Effects of intrathecal administration of ziconotide, a selective neuronal N-type calcium channel blocker, on mechanical allodynia and heat hyperalgesia in a rat model of postoperative pain
    Y X Wang
    Department of Pharmacology, Elan Pharmaceuticals, 3760 Haven Avenue, Menlo Park, CA 94025, USA
    Pain 84:151-8. 2000
    ..The results of this study show that intrathecal ziconotide is antinociceptive in a rat incisional model of post-operative pain and is more potent, longer acting, and more specific in its actions than intrathecal morphine...
  64. ncbi Properties of human Cav2.1 channel with a spinocerebellar ataxia type 6 mutation expressed in Purkinje cells
    Hironao Saegusa
    Department of Pharmacology and Neurobiology, Graduate School of Medicine, Tokyo Medical and Dental University, 1 5 45 Yushima, Tokyo 113 8519, Japan
    Mol Cell Neurosci 34:261-70. 2007
    ..Therefore, our results do not support the notion that the alteration of the channel properties may underlie the pathogenic mechanism of SCA6...
  65. ncbi Alternative splicing of a beta4 subunit proline-rich motif regulates voltage-dependent gating and toxin block of Cav2.1 Ca2+ channels
    Thomas D Helton
    Department of Molecular Biomedical Sciences, North Carolina State University, Raleigh, North Carolina 27606, USA
    J Neurosci 22:9331-9. 2002
    ..These results raise interesting questions about the functional role that alternative splicing of the beta4 subunit has played in the evolution of complex neural networks...
  66. ncbi Systematic identification of splice variants in human P/Q-type channel alpha1(2.1) subunits: implications for current density and Ca2+-dependent inactivation
    Tuck Wah Soong
    National Neuroscience Institute, Singapore 308433
    J Neurosci 22:10142-52. 2002
    ..These findings significantly expand the anticipated scope of functional diversity produced by splice variation of P/Q-type channels...
  67. pmc Differential modulation of N-type 1B and P/Q-type 1A calcium channels by different G protein subunit isoforms
    M I Arnot
    Neuroscience and Smooth Muscle Research Groups, Department of Pharmacology and Therapeutics, University of Calgary, Calgary, Canada
    J Physiol 527:203-12. 2000
    ..The differential modulation of P/Q- and N-type channels by various Gbeta subtypes may provide a mechanism for fine tuning the amount of calcium entering the presynaptic nerve termini...
  68. ncbi Effects of glucocorticoid receptor antagonists on allodynia and hyperalgesia in mouse model of neuropathic pain
    Ichiro Takasaki
    Department of Pharmacology and Neurobiology, Graduate School of Medicine, Tokyo Medical and Dental University, Bunkyo ku, Japan
    Eur J Pharmacol 524:80-3. 2005
    ..These results suggest that spinal glucocorticoid receptors play an important role in neuropathic pain, and that controlling the activity of glucocorticoid receptors may be of great importance in the treatment of neuropathic pain...
  69. pmc Functional architecture of the inner pore of a voltage-gated Ca2+ channel
    Xiao guang Zhen
    Department of Biological Sciences, Columbia University, New York, NY 10027, USA
    J Gen Physiol 126:193-204. 2005
    ..This work provides an impetus for future studies on ion permeation, gating, and drug binding of VGCCs...
  70. ncbi Alternative splicing in the cytoplasmic II-III loop of the N-type Ca channel alpha 1B subunit: functional differences are beta subunit-specific
    J Q Pan
    Department of Neuroscience, Brown University, Providence, Rhode Island 02912, USA
    J Neurosci 20:4769-75. 2000
    ..The potential to mix and match multiple alpha(1B) splice variants and beta subunits probably represents a mechanism for controlling the plasticity of excitation-secretion coupling at different synapses...
  71. pmc Spinocerebellar ataxia type 6 knockin mice develop a progressive neuronal dysfunction with age-dependent accumulation of mutant CaV2.1 channels
    Kei Watase
    Twenty First Century Center of Excellence Program on Brain Integration and Its Disorders, Tokyo Medical and Dental University, 1 5 45 Yushima, Bunkyo ku, Tokyo 113 8519, Japan
    Proc Natl Acad Sci U S A 105:11987-92. 2008
    ..The pathogenesis of SCA6 is apparently linked to an age-dependent process accompanied by accumulation of mutant Ca(V)2.1 channels...
  72. ncbi G-protein inhibition of N- and P/Q-type calcium channels: distinctive elementary mechanisms and their functional impact
    H M Colecraft
    Program in Molecular and Cellular Systems Physiology, Departments of Biomedical Engineering and Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Neurosci 21:1137-47. 2001
    ..Variable expression of N- and P/Q-type channels at spatially distinct synapses therefore offers the potential for custom regulation of both synaptic efficacy and synchrony, by G-protein inhibition...
  73. pmc Orientation of the calcium channel beta relative to the alpha(1)2.2 subunit is critical for its regulation of channel activity
    Iuliia Vitko
    Department of Pharmacology and Neuroscience Graduate Program, University of Virginia, Charlottesville, Virginia, USA
    PLoS ONE 3:e3560. 2008
    ..One hypothesis is that betas regulate channel gating by modulating movements of IS6. A key requirement for this direct-coupling model is that the linker connecting IS6 to the alpha-interaction domain (AID) be a rigid structure...
  74. ncbi beta subunit reshuffling modifies N- and P/Q-type Ca2+ channel subunit compositions in lethargic mouse brain
    D L Burgess
    Department of Neurology, Baylor College of Medicine, Houston, Texas 77030, USA
    Mol Cell Neurosci 13:293-311. 1999
    ..The existence of beta subunit reshuffling demonstrates that molecular plasticity of Ca2+ channel assembly, a normal feature of early brain development, is retained in the mature brain...
  75. ncbi Targeting Ca2+ channels to treat pain: T-type versus N-type
    Christophe Altier
    Department of Physiology and Biophysics, Cellular and Molecular Neurobiology Research Group, University of Calgary, Calgary, Alberta T2N 4N1, Canada
    Trends Pharmacol Sci 25:465-70. 2004
    ..In this review, we discuss the implications of these findings for the design of novel therapeutic strategies and contrast the role of T-type channels with that of N-type channels in pain transmission and analgesia...
  76. pmc Orientation of palmitoylated CaVbeta2a relative to CaV2.2 is critical for slow pathway modulation of N-type Ca2+ current by tachykinin receptor activation
    Tora Mitra-Ganguli
    Department of Physiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    J Gen Physiol 134:385-96. 2009
    ..These findings support our previous hypothesis that Ca(V)beta2a's palmitoyl groups directly interact with an inhibitory site on Ca(V)2.2 to block N-current inhibition by SP...
  77. pmc The Ca2+ channel beta subunit determines whether stimulation of Gq-coupled receptors enhances or inhibits N current
    John F Heneghan
    Department of Physiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    J Gen Physiol 134:369-84. 2009
    ..Moreover, these findings predict that at synapses, modulation of N-channel activity by M(1)Rs or NK-1Rs will fluctuate between enhancement and inhibition based on the presence of palmitoylated Ca(V)beta2a...
  78. ncbi Binding of G alpha(o) N terminus is responsible for the voltage-resistant inhibition of alpha(1A) (P/Q-type, Ca(v)2.1) Ca(2+) channels
    M Kinoshita
    Department of Neuropharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
    J Biol Chem 276:28731-8. 2001
    ..These findings demonstrate that the voltage-resistant inhibition of the P/Q-type, alpha(1A) channel is caused by the interaction between the C-terminal domain of Ca(2+) channel alpha(1A) subunit and the N-terminal region of G alpha(o)...
  79. ncbi PKCepsilon upregulates voltage-dependent calcium channels in cultured astrocytes
    M Burgos
    Unidad de Fisiologia, Facultad de Medicina y Centro Regional de Investigaciones Biomedicas, Universidad de Castilla La Mancha, Albacete, Spain
    Glia 55:1437-48. 2007
    ..Therefore, here we describe a novel regulatory pathway involving VGCC that participates in PKCepsilon-dependent astrocyte activation...
  80. ncbi PMA counteracts G protein actions on CaV2.2 channels in rat sympathetic neurons
    Alfonso F Díaz-Cárdenas
    Departamento de Fisiologia, Facultad de Medicina, Universidad Nacional Autonoma de Mexico, Av Universidad 3000, Ciudad Universitaria, Delegación Coyoacán C P 04510, Apdo Postal 70250, Mexico, DF, Mexico
    Arch Biochem Biophys 473:1-7. 2008
    ..Furthermore, counteracting modulation of ion channels and reversibility within a short time frame are better support for a dynamic system with short-term adaptive responses...
  81. pmc The tumor suppressor eIF3e mediates calcium-dependent internalization of the L-type calcium channel CaV1.2
    Eric M Green
    Department of Neurobiology, Stanford University, 299 Campus Drive, Fairchild Research Building D227, Stanford, CA 94305, USA
    Neuron 55:615-32. 2007
    ..These findings provide a mechanism for activity-dependent internalization and trafficking of CaV1.2 and provide a tantalizing link between Ca2+ homeostasis and a mammalian oncogene...
  82. ncbi 5-HT inhibits N-type but not L-type Ca(2+) channels via 5-HT1A receptors in lamprey spinal neurons
    Russell H Hill
    Nobel Institute for Neurophysiology, Department of Neuroscience, Karolinska Institutet, S 17177 Stockholm, Sweden
    Eur J Neurosci 18:2919-24. 2003
    ..As N-type, but not L-type, Ca(2+) channels are known to mediate the activation of KCa channels and presynaptic transmitter release at lamprey synapses, the effects of 5-HT reported here can contribute to a reduction in both actions...
  83. ncbi D1 receptors physically interact with N-type calcium channels to regulate channel distribution and dendritic calcium entry
    Alexandra E Kisilevsky
    Department of Physiology and Biophysics, Hotchkiss Brain Institute, University of Calgary, 3330 Hospital Drive Northwest, Calgary, Canada
    Neuron 58:557-70. 2008
    ..Our data indicate that the N-type calcium channel is an important physiological target of D1 receptors and reveal a mechanism for D1 receptor-mediated regulation of cognitive function in the PFC...
  84. ncbi Low-threshold L-type calcium channels in rat dopamine neurons
    P Durante
    Department of Anatomy and Neurobiology, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA
    J Neurophysiol 91:1450-4. 2004
    ....
  85. ncbi Calcium channels involved in noradrenaline release from sympathetic neurones in rabbit carotid artery
    Torben R Uhrenholt
    Department of Physiology and Pharmacology, University of Southern Denmark, Odense, Denmark
    Pharmacol Toxicol 92:226-33. 2003
    ....
  86. ncbi Compensatory increase in P/Q-calcium current-mediated synaptic transmission following chronic block of N-type channels
    Christiane Grimm
    Department of Physiology and Pathophysiology, University of Heidelberg, INF 326, 69120 Heidelberg, Germany
    Neurosci Lett 442:44-9. 2008
    ..1, the underlying calcium channel alpha-subunit. We conclude that developing hippocampal neurons can compensate for the loss of one calcium current component by up-regulation of alternative isoforms at the post-translational level...
  87. ncbi Neurotensin stimulates both calcium mobilization from inositol trisphosphate-sensitive intracellular stores and calcium influx through membrane channels in frog pituitary melanotrophs
    Amor Belmeguenai
    European Institute for Peptide Research IFRMP 23, Laboratory of Cellular and Molecular Neuroendocrinology, Institut National de la Sante et de la Recherche Medicale, Unité 413, University of Rouen, 76821 Mont Saint Aignan, France
    Endocrinology 144:5556-67. 2003
    ..In addition, the present data indicate that PKC plays a crucial role in maintenance of the responsiveness of melanotrophs to NT...
  88. pmc Neurotransmitter modulation of small-conductance Ca2+-activated K+ channels by regulation of Ca2+ gating
    Francois Maingret
    Centre de Recherche en Neurobiologie et Neurophysiologie de Marseille CRN2M, CNRS UMR 6231, Universite de la Mediterranee, Boulevard Pierre Dramard, 13916, Marseille Cedex 20, France
    Neuron 59:439-49. 2008
    ..Hence, neurotransmitter-initiated signaling cascades can dynamically regulate Ca2+ sensitivity of SK channels and directly influence somatic excitability...
  89. ncbi Mechanisms of sodium azide-induced changes in intracellular calcium concentration in rat primary cortical neurons
    Silvia Marino
    Department of Clinical and Experimental Medicine, Section of Pharmacology, University of Ferrara, Via Fossato di Mortara 17 19, 44100 Ferrara, Italy
    Neurotoxicology 28:622-9. 2007
    ..These findings show that the predominant source of calcium increase induced by NaN(3) is extracellular, involving glutamate receptor activation in a first step and calcium channel (mainly of the N-type) opening in a second step...
  90. ncbi Differential contribution of L-, N-, and P/Q-type calcium channels to [Ca2+]i changes evoked by kainate in hippocampal neurons
    Ana R Santiago
    Center for Neuroscience and Cell Biology, Department of Zoology, University of Coimbra, Coimbra, 3004 517, Portugal
    Neurochem Res 33:1501-8. 2008
    ..The results indicate that hippocampal neurons differ in what concerns their L-, N- and P/Q-type Ca(2+) channels activated by stimulation of the AMPA/kainate receptors...
  91. ncbi High-voltage-activated calcium channels in Muller cells acutely isolated from tiger salamander retina
    Nicole C Welch
    Department of Pharmacology, Dalhousie University, Halifax, Nova Scotia, Canada
    Glia 49:259-74. 2005
    ..Overall, these observations suggest either the presence of a mix of L- and N-type Ca channels or a single, unconventional HVA Ca channel subtype sharing L- and N-type Ca channel characteristics...
  92. ncbi Spontaneous muscle action potentials are blocked by N-type and P/Q-calcium channels blockers in the rat spinal cord-muscle co-culture system
    Kyoji Taguchi
    Department of Neuroscience, Showa Pharmaceutical University, 3 3165 Higashitamagawagakuen, Machida, Tokyo 194 8543, Japan
    Brain Res 1034:62-70. 2005
    ..These results indicate that blockers of N- and P/Q-type voltage-dependent calcium channels inhibit transmitter release at neuromuscular junctions in the rat spinal cord-muscle co-culture system...
  93. ncbi A protein phosphatase 2calpha-Ca2+ channel complex for dephosphorylation of neuronal Ca2+ channels phosphorylated by protein kinase C
    Dongjun Li
    Department of Physiology, Jefferson Medical College, Philadelphia, Pennsylvania 19107, USA
    J Neurosci 25:1914-23. 2005
    ..Thus, the PP2calpha-Ca2+ channel complex is responsible for rapid dephosphorylation of Ca2+ channels and may contribute to regulation of synaptic transmission in neurons...
  94. ncbi Inhibitory effect of pranidipine on N-type voltage-dependent Ca2+ channels in mice
    Manabu Murakami
    Department of Pharmacology, Akita University School of Medicine, 1 1 1 Hondoh, Akita 010 8543, Japan
    Neurosci Lett 367:118-22. 2004
    ....
  95. ncbi Extracellular domains of alpha-neurexins participate in regulating synaptic transmission by selectively affecting N- and P/Q-type Ca2+ channels
    Weiqi Zhang
    Center for Physiology and Pathophysiology, Georg August University, D 37073 Gottingen, Germany
    J Neurosci 25:4330-42. 2005
    ....
  96. ncbi Voltage-gated calcium channels in developing GnRH-1 neuronal system in the mouse
    Yoko Toba
    Cellular and Developmental Neurobiology Section, NINDS, NIH, Bethesda, MD 20892, USA
    Eur J Neurosci 22:79-92. 2005
    ..These results indicate that extracellular calcium is required for initiating GnRH-1 neuronal migration and that these events are partially dependent on N-type VGCC signals...
  97. ncbi [Dosage and specificity of anti-calcium channel antibodies in Lambert-Eaton myasthenic syndrome]
    N Martin-Moutot
    UMR 641 INSERM Université de la Méditerranée, IFR Jean Roche, Faculte de Medecine Secteur Nord, Marseille, France
    Rev Neurol (Paris) 160:S28-34. 2004
    ..Only 5 patients were seronegative in both tests, thus a combination of the two assays reliably detected autoantibodies in 26/31 (84%) patients...
  98. ncbi Intracellular calcium increase in epileptiform activity: modulation by levetiracetam and lamotrigine
    Antonio Pisani
    Clinica Neurologica, Dipartimento di Neuroscienze, Universita di Roma Tor Vergata, Rome, Italy
    Epilepsia 45:719-28. 2004
    ..Levetiracetam (LEV) and lamotrigine (LTG), novel antiepileptic drugs (AEDs), were tested on epileptiform events and the corresponding elevations in intracellular Ca2+ concentration ([Ca2+]i) recorded from rat neocortical slices...
  99. ncbi Gamma-aminobutyric acid type B receptors facilitate L-type and attenuate N-type Ca(2+) currents in isolated hippocampal neurons
    Thomas J Carter
    Department of Biological Sciences, Marquette University, Milwaukee, Wisconsin 53201 1881, USA
    J Neurosci Res 76:323-33. 2004
    ..The two opposing effects of GABA(B) receptor activation may reflect the heterogeneity of the cultured cells or may be a developmentally regulated phenomenon...
  100. ncbi Functional compensation by other voltage-gated Ca2+ channels in mouse basal forebrain neurons with Ca(V)2.1 mutations
    Jason A Etheredge
    Department of Medical Pharmacology and Toxicology, College of Medicine, Reynolds Medical Science Building, Texas A and M University System Health Science Center, 1114 TAMU, College Station, TX 77843 1114, USA
    Brain Res 1140:105-19. 2007
    ....
  101. ncbi Effects of 50 Hz electromagnetic fields on voltage-gated Ca2+ channels and their role in modulation of neuroendocrine cell proliferation and death
    Claudio Grassi
    Institute of Human Physiology, Medical School, Catholic University S Cuore, Largo F Vito 1, 00168 Rome, Italy
    Cell Calcium 35:307-15. 2004
    ..The consequent increase in Ca(2+) influx is likely responsible for the EF-induced modulation of neuronal cell proliferation and apoptosis...

Research Grants62

  1. PRESYNAPTIC RECEPTORS ON EMBRYONIC SENSORY NEURONS
    Kathleen Dunlap; Fiscal Year: 2007
    ..g., allodynia and hypersensitivity- identifying mechanisms that control Ca2+ influx in sensory neurons may allow development of new therapeutic strategies to treat chronic pain. ..
  2. Nitrergic Neuro-smooth Muscle Transmission in the Gut
    Raj K Goyal; Fiscal Year: 2013
    ..This proposal is to understand how these disorders are caused and how better treatments for these disorders can be developed. ..
  3. Regulation of (Ca2+)i oscillation and osteoclast differentiation by RGS12
    Shuying Yang; Fiscal Year: 2010
    ..Aim 3. To define the role and mechanism of RGS12 in vivo in bone remodeling by osteoclast-specific targeted disruption of the RGS12 gene using Cre/loxP technology! ..
  4. N-type Calcium Channels in Nociceptive Neurons
    Diane Lipscombe; Fiscal Year: 2013
    ..By studying a novel form of the N-type calcium channel in the pain pathway, our studies of novel knock-in mice should lead to new strategies for inhibiting the activity of this N-type calcium channel and alleviating pain sufferers. ) ..
  5. Regulation and function of hippocampal excrescences
    Daniel T Pak; Fiscal Year: 2011
    ..abstract_text> ..
  6. PATCH CLAMP STUDY OF GASTROINTESTINAL NEURONS
    JACKIE WOOD; Fiscal Year: 1999
    ....
  7. Farnesol Analogues as Novel Treatment of Alcoholism
    Jean Baptiste Roullet; Fiscal Year: 2005
    ..Potential commercialization: This research will provide the foundation for Phase II evaluation of the most active farnesol analogue in the treatment of alcohol withdrawal syndrome in alcoholic patients. ..
  8. STUDIES OF N-TYPE CALCIUM CHANNELS
    Aaron Fox; Fiscal Year: 2003
    ....
  9. N TYPE CA CHANNELS IN GROWTH CONES
    Maureen McEnery; Fiscal Year: 2000
    ....
  10. CALCIUM CHANNELS IN SYMPATHETIC NEURONS
    KEITH ELMSLIE; Fiscal Year: 2000
    ..In addition, the knowledge gained about single N-channel gating will be important for the development of comprehensive models on calcium-dependent synaptic release and how that release can be inhibited by neurotransmitters. ..
  11. NEUROTRANSMITTERS AND BRAIN INJURY
    MARK ZORNOW; Fiscal Year: 2000
    ..These studies will provide a better understanding of the mechanisms involved in neurologic injury and will help to resolve questions regarding the possible therapeutic benefits of these potential neuroprotective agents. ..
  12. CALCIUM CHANNELS ON SYNAPTIC TRANSMISSION
    D Kim; Fiscal Year: 1999
    ....
  13. Development of High Throughput Assays for N-type Calcium Channels
    Edward Perez Reyes; Fiscal Year: 2007
    ..By targeting an important protein in the pain pathway, the research funded by this grant will provide tools that can be used to screen candidate compounds during the development of novel analgesics. [unreadable] [unreadable] [unreadable]..
  14. NEURONAL CALCIUM CHANNELS--REGULATION AND FUNCTION
    Diane Lipscombe; Fiscal Year: 2000
    ..This award is requested at a critical time for Dr. Lipscombe to devote herself fully to projects which are already providing new and important insights into Ca2+ channel function. The proposed plan is fully endorsed by Brown University. ..
  15. MOLECULAR MECHANISMS OF N TYPE CALCIUM CHANNEL MODULATIO
    STEPHEN IKEDA; Fiscal Year: 2001
    ....
  16. NOVEL NEUROPROTECTIVE AGENTS
    PAUL MARANGOS; Fiscal Year: 1991
    ..Phase II - We will launch a medicinal chemistry effort to derivatize the agent characterized in phase I. The goals here are to generate agents with greater potency at the n-type calcium channel...
  17. PKC epsilon Regulation of Cannabinoid Response
    MELISA WALLACE; Fiscal Year: 2007
    ..These investigations will not only provide insight into a factor that may contribute to marijuana dependence, but will also aid in understanding regulatory mechanisms of the endogenous cannabinoid system. ..
  18. Screen:Blockers of a CaV2.2-Mint-PDZ1 association (RMI)
    Ilya Bezprozvanny; Fiscal Year: 2004
    ..Biological activity of compounds identified in the full HTS screen will be tested in whole animal pain assays (formalin, hot plate, tail flick). ..
  19. Enkephalins: Neuropharmacology and Abuse Potential
    William Dewey; Fiscal Year: 2006
    ....
  20. Neuropeptides and the Mammalian Circadian System
    Christopher Colwell; Fiscal Year: 2007
    ..In addition, transgenic mice lacking PACAP and VIP will be analyzed with behavioral and anatomical tools. These newly developed mice are likely to prove a useful tool for circadian rhythms research. ..
  21. Neuromodulation of Voltage-gated Ca2+ Channels
    Jian Yang; Fiscal Year: 2007
    ..This research may not only enhance our understanding of the complex regulatory pathways for VGCCs but also provide valuable information for the development of potential therapeutic reagents that target these pathways ..
  22. CA CHANNELS: G PROTEINS & INACTIVATION
    David Yue; Fiscal Year: 2001
    ..Because P/Q and N type calcium channels trigger neurotransmitter release, dynamic modulation of channels by G proteins and voltage inactivation ..
  23. REGULATION OF THE MAMMALIAN CIRCADIAN SYSTEM
    Christopher Colwell; Fiscal Year: 2002
    ..Finally, the proposed work will determine whether a model developed to explain synchronization of molluscan circadian oscillators can be applied to the mammalian SCN. ..
  24. Confocal Microscope for Mutant-GFP-Based FRET
    David Yue; Fiscal Year: 2002
    ..The microscope will anchor a departmental facility supported by 3 major and 3 minor users, all of whose research would be fundamentally advanced by the platform's unique capabilities. ..
  25. MOLECULAR PHYSIOLOGY OF INWARDLY RECTIFYING K CHANNELS
    Jian Yang; Fiscal Year: 2001
    ..They will also allow us to gain better insight into the molecular architecture of the channel pore, which may help in the development of pharmacological agents directed at these channels. ..
  26. Characterization of N-type Ca channels in growth cones
    Maureen McEnery; Fiscal Year: 2005
    ....
  27. A role for circadian clock genes in hippocampal function?
    Christopher Colwell; Fiscal Year: 2008
    ..This line of research has not been previously funded, is exploratory in nature, and thus qualifies under the R21 format. [unreadable] [unreadable] [unreadable]..
  28. Plasticity of Kappa Opioid Function in Reward Circuitry
    GREGORY HJELMSTAD; Fiscal Year: 2007
    ..Specific aims will test both the extracellular and intracellular mechanisms underlying this effect, as well as testing the specificity of the effect on glutamate release. ..
  29. Genetics of Familial Episodic Ataxia
    Joanna C Jen; Fiscal Year: 2010
    ..Insights gained from the study will lead to improved diagnosis and treatment of familial episodic ataxia and the more common episodic vertigo syndromes. ..
  30. Cortical Spreading Depression, Proteases and Ischemia
    Michael Moskowitz; Fiscal Year: 2008
    ..By doing so, we intend to explore ways in which neuronal activity during cerebral ischemia modulates the extracellular matrix and neurovascular unit and contribute to tissue injury. ..
  31. AFFERENT PLASTICITY UNDERLYING URETHRAL AND PELVIC PAIN
    Naoki Yoshimura; Fiscal Year: 2010
    ..This is recognized as a high priority in the urologic care of patients with IC/CPPS. ..
  32. Central contributions to pathobiology of fibromyalgia
    Kathleen Sluka; Fiscal Year: 2009
    ..A better understanding of the pathobiological mechanisms underlying musculoskeletal pain conditions may lead to the development of novel therapeutic approaches for its treatment. ..
  33. CEREBRAL VESSEL INNERVATION IN HYPERTENSION
    Tony Lee; Fiscal Year: 2002
    ..This research is a step toward our long-term goal to define the transmitter mechanisms in cerebral vasodilation and constriction, and their alterations in cerebral vascular diseases. ..
  34. HMG1 SIGNALING IN INFLAMMATION FOLLOWING BRAIN ISCHEMIA
    Michael Moskowitz; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  35. MUTATIONS IN CALCIUM CHANNELS CAUSING VERTIGO AND ATAXIA
    Joanna Jen; Fiscal Year: 2002
    ..The further relevance of this work to the more common basilar migraine and Meniere's syndrome is emphasized by the overlapping symptoms of vertigo and ataxia in the group of patients that we propose to study. ..
  36. Afferent modulation in bladde dysfunction
    Naoki Yoshimura; Fiscal Year: 2007
    ..abstract not provided ..
  37. Caspase Mechanisms after Brain Injury
    Michael Moskowitz; Fiscal Year: 2006
    ..Together these experiments will explore death receptor-mediated acute cell killing within brain in order to identify mechanisms and potential targets of ischemic and traumatic brain injury. ..
  38. Neurophysiology and Biomechanics of Urethra in Stress Urinary Incontinence
    Naoki Yoshimura; Fiscal Year: 2008
    ..This is recognized as a high priority in the urologic/gynecologic care of SUI patients. [unreadable] [unreadable]..
  39. Synaptic Defects in the Ca Channel Mutant Mouse
    Kathleen Dunlap; Fiscal Year: 2004
    ....
  40. Modulation and Targeting of Kainate Receptors
    John Marshall; Fiscal Year: 2004
    ..unclustered) will be evaluated. These studies are expected to provide novel information on how functional properties can be determined through mechanisms controlling the clustering/targeting of receptors of specific subunit composition. ..
  41. Bladder Pain Gene Therapy with Pro-opiomelanocortin cDNA
    Naoki Yoshimura; Fiscal Year: 2004
    ..This research project is important to provide a solid basis for potential future clinical application. ..
  42. G-protein alpha subunits in regulation of GIRK channels
    Nathan Dascal; Fiscal Year: 2006
    ..Colocalization and complex formation between Galpha and GIRK will be explored in atrium and brain using biochemical and immunohistochemical methods. ..
  43. PAIN EVOKED RELEASE OF AMINO ACIDS AND PROSTANOIDS
    Tony Yaksh; Fiscal Year: 2005
    ....
  44. Spinal Galanin and its Receptors in Pain Processing
    Tony Yaksh; Fiscal Year: 2005
    ..We believe the outcome of these studies will provide direct evidence for the role played by this spinal peptidergic system in pain and point to the development of novel anti-hyperpathic agents. ..
  45. Properties of calcium channel mutants linked to migraine
    Yuqing Cao; Fiscal Year: 2004
    ..This work may provide useful clues about the dominant pattern of inheritance of FHM as well as shed light on the mechanism underlying other forms migraine. ..
  46. INTERDEPARTMENTAL STROKE PROGRAM
    Michael Moskowitz; Fiscal Year: 2008
    ..abstract_text> ..
  47. CRCNS:Comput./Exp.Study:Hypothal.-Pituitary Interaction
    Richard Bertram; Fiscal Year: 2008
    ..It will then lead to studies of effects of drugs of abuse on brain-pituitary function. [unreadable] [unreadable]..
  48. Photothrombotic Brain Infarction and Epileptogenesis
    Kevin Kelly; Fiscal Year: 2005
    ..abstract_text> ..
  49. Characterization of Toxicity with Spinal Opiates
    Tony L Yaksh; Fiscal Year: 2010
    ..The present studies aim to define the mechanisms of granuloma formation and point to specific methods to prevent granuloma formation, which will increase the utility and reduce the risks of this important pain therapy. ..
  50. Mutants Showing SLO-1 Synaptic Mislocalization
    Zhao Wen Wang; Fiscal Year: 2005
    ..elegans. Analysis of these mutants in subsequent studies may allow the identification of molecules mediating SLO-1 synaptic targeting and localization. ..
  51. Sensitization in Trigeminal Neurons: A Role in Migraine?
    JOYCE HURLEY; Fiscal Year: 2008
    ..abstract_text> ..
  52. Protein ubiquitination based neuroprotection for stroke
    Robert Meller; Fiscal Year: 2006
    ....
  53. Coupling mechanisms of OP4 receptor and calcium channels
    Victor Ruiz Velasco; Fiscal Year: 2006
    ..Moreover, the information gathered from these studies may help determine the precise role opioids play in decreasing myocardial infarct size. [unreadable] [unreadable] [unreadable]..
  54. Nasal Delivery of Naltrexone for Treatment of Alcoholism
    Daniel Wermeling; Fiscal Year: 2006
    ..The long-term goal of this work is to develop and commercialize nasal delivered NTX for the treatment of alcoholism. [unreadable] [unreadable] [unreadable]..
  55. Synaptic change in cingulate cortex after amputation
    Min Zhuo; Fiscal Year: 2006
    ..This information will provide a potential neuronal basis for understanding phantom limb sensation and phantom pain. ..
  56. International Conference on Episodic Ataxia Syndromes
    Joanna Jen; Fiscal Year: 2005
    ..abstract_text> ..
  57. MIGRAINE DRUG PROPHYLAXIS
    Michael Moskowitz; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  58. Lower urinary tract dysfunction in Parkinson's disease
    Naoki Yoshimura; Fiscal Year: 2003
    ..This is a high priority in the urologic care of patients with Parkinson's disease. ..
  59. Rapid ischemic tolerance: Ubiquitin-mediated structural reorganization
    Robert Meller; Fiscal Year: 2007
    ..Indeed, the long-term aim of these studies is to discover endogenous protective mechanisms that can be translated into effective rapid acting neuroprotective agents for stroke. [unreadable] [unreadable] [unreadable]..
  60. Pain Mechanisms and the Development of Analgesics
    Tony Yaksh; Fiscal Year: 2006
    ..g. COX2 inhibitors, GABApentin, ziconotide) and failures (NK1 antagonist). [unreadable] [unreadable] [unreadable]..
  61. THE USE OF ELECTROACUPUNCTURE TO MODULATE AROUSAL
    Edgar Garcia Rill; Fiscal Year: 2002
    ..However, the optimal stimulation sites and paradigms muse be identified and proven effective, first, in control populations, and later, in pathological populations. ..
  62. Homologous Recombination in a Secretory Mouse Cell Line
    AMY HARKINS; Fiscal Year: 2003
    ....