nucleobase transport proteins

Summary

Summary: Proteins involved in the transport of nucleobases such as PYRIMIDINES and PURINES across membranes.

Top Publications

  1. ncbi Cloning, heterologous expression, and in situ characterization of the first high affinity nucleobase transporter from a protozoan
    Richard J S Burchmore
    Institute of Biomedical and Life Sciences, Division of Infection and Immunity, University of Glasgow, Glasgow G12 8QQ, United Kingdom
    J Biol Chem 278:23502-7. 2003
  2. ncbi Purine and pyrimidine transport in pathogenic protozoa: from biology to therapy
    Harry P de Koning
    Institute of Biomedical and Life Sciences, Division of Infection and Immunity, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, UK
    FEMS Microbiol Rev 29:987-1020. 2005
  3. doi Specific interdomain synergy in the UapA transporter determines its unique specificity for uric acid among NAT carriers
    Ioannis Papageorgiou
    Faculty of Biology, University of Athens, Panepistimioupolis 15781, Athens, Greece
    J Mol Biol 382:1121-35. 2008
  4. ncbi Purine nucleobase transport in the intraerythrocytic malaria parasite
    Megan J Downie
    School of Biochemistry and Molecular Biology, Faculty of Science, The Australian National University, Canberra, ACT 0200, Australia
    Int J Parasitol 38:203-9. 2008
  5. doi Genetic and molecular characterization reveals a unique nucleobase cation symporter 1 in Arabidopsis
    George S Mourad
    Department of Biology, Indiana University Purdue University Fort Wayne, Fort Wayne, IN 46805, USA
    FEBS Lett 586:1370-8. 2012
  6. pmc Fluorescence monitoring of riboswitch transcription regulation using a dual molecular beacon assay
    Raja Chinnappan
    Department of Biology, Faculty of Sciences, RNA Group, Universite de Sherbrooke, Sherbrooke, Quebec J1K 2R1, Canada
    Nucleic Acids Res 41:e106. 2013
  7. pmc Substrate selectivity of YgfU, a uric acid transporter from Escherichia coli
    Konstantinos Papakostas
    Laboratory of Biological Chemistry, University of Ioannina Medical School, 45110 Ioannina, Greece
    J Biol Chem 287:15684-95. 2012
  8. pmc De novo pyrimidine nucleotide synthesis mainly occurs outside of plastids, but a previously undiscovered nucleobase importer provides substrates for the essential salvage pathway in Arabidopsis
    Sandra Witz
    Abteilung Pflanzenphysiologie, Fachbereich Biologie, Technische Universitat Kaiserslautern, D 67663 Kaiserslautern, Germany
    Plant Cell 24:1549-59. 2012
  9. pmc Loss of heterozygosity of FCY2 leading to the development of flucytosine resistance in Candida tropicalis
    Yen Ning Chen
    Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan, Republic of China
    Antimicrob Agents Chemother 55:2506-14. 2011
  10. ncbi Fungal nucleobase transporters
    Areti Pantazopoulou
    Faculty of Biology, Department of Botany, University of Athens, Panepistimioupolis, Athens, Greece
    FEMS Microbiol Rev 31:657-75. 2007

Scientific Experts

  • Harry P de Koning
  • George S Mourad
  • Mark J Wall
  • P Dessen
  • Javier Valdez-Taubas
  • M A Sanchez
  • Scott Landfear
  • William W Hope
  • VIVIAN M BELLOFATTO
  • Thierry Noel
  • George Diallinas
  • Stathis Frillingos
  • Sotiris Amillis
  • Konstantinos Papakostas
  • Panayiota Karatza
  • Derek B J Bone
  • Ekaterini Georgopoulou
  • Areti Pantazopoulou
  • Diana Ortiz
  • Ryo Kato
  • John P Paluszynski
  • Ekaterini Karena
  • Christos Gournas
  • Anna Vlanti
  • Ioannis Papageorgiou
  • Mohammed I Al-Salabi
  • T Ferreira
  • Anna Cedzich
  • D Brethes
  • Raja Chinnappan
  • Sandra Witz
  • J Chevallier
  • C Napias
  • Yen Ning Chen
  • Lynsey J M Wallace
  • Neils B Quashie
  • Syunsuke Yamamoto
  • Tyler A Mansfield
  • James R Hammond
  • Megan J Downie
  • Kazi Mirajul Hoque
  • Simone Weyand
  • Ikumi Tamai
  • Toshihiro Akaike
  • Tomoji Maeda
  • Friedhelm Meinhardt
  • Roland Klassen
  • B Pinson
  • Veronica G Maurino
  • Wolf B Frommer
  • Claudio Scazzocchio
  • Harald Stransky
  • Manal J Natto
  • Florence Chapeland-Leclerc
  • Sophia Goudela
  • I G Papageorgiou
  • Marina Koukaki
  • L J M Wallace
  • Denise Candlish
  • Cristina Henriques
  • Lukas Bürkle
  • Richard J S Burchmore
  • A Theisinger
  • G Diallinas
  • Maria Fedorova
  • Jean François Lemay
  • Audrey Dubé
  • Daniel A Lafontaine
  • R Wagner
  • Torsten Möhlmann
  • Sarah Fürst
  • Benjamin Jung
  • M F Vickers
  • Vasiliki Kosti
  • Te Pin Chang
  • Chia Chen Wu
  • Hsiu Jung Lo
  • Hui Ching Ko
  • Yun Liang Yang
  • Emmanuel Mikros
  • Katsuhisa Inoue
  • George Mermelekas
  • Hiroaki Yuasa
  • Imogen R Coe
  • Tomoya Yasujima
  • Yukihiro Yoshida
  • Jun ya Maeda
  • Kin ya Ohta
  • Doo Sup Choi
  • Lisa C Ranford-Cartwright

Detail Information

Publications70

  1. ncbi Cloning, heterologous expression, and in situ characterization of the first high affinity nucleobase transporter from a protozoan
    Richard J S Burchmore
    Institute of Biomedical and Life Sciences, Division of Infection and Immunity, University of Glasgow, Glasgow G12 8QQ, United Kingdom
    J Biol Chem 278:23502-7. 2003
    ..Expression of TbNBT1 in Xenopus oocytes further confirmed that this gene encodes the first high affinity nucleobase transporter from protozoa or animals to be identified at the molecular level...
  2. ncbi Purine and pyrimidine transport in pathogenic protozoa: from biology to therapy
    Harry P de Koning
    Institute of Biomedical and Life Sciences, Division of Infection and Immunity, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, UK
    FEMS Microbiol Rev 29:987-1020. 2005
    ..Recent studies are increasingly addressing the structure and substrate recognition mechanisms of these vital transport proteins...
  3. doi Specific interdomain synergy in the UapA transporter determines its unique specificity for uric acid among NAT carriers
    Ioannis Papageorgiou
    Faculty of Biology, University of Athens, Panepistimioupolis 15781, Athens, Greece
    J Mol Biol 382:1121-35. 2008
    ..We finally show that intramolecular synergy among UapA domains is highly specific and propose that it forms the basis for the evolution of the unique specificity of UapA for uric acid, a property not present in other NAT members...
  4. ncbi Purine nucleobase transport in the intraerythrocytic malaria parasite
    Megan J Downie
    School of Biochemistry and Molecular Biology, Faculty of Science, The Australian National University, Canberra, ACT 0200, Australia
    Int J Parasitol 38:203-9. 2008
    ..The results indicate that nucleobases enter the intraerythrocytic parasite via a rapid, equilibrative process that has kinetic characteristics similar to those of PfENT1...
  5. doi Genetic and molecular characterization reveals a unique nucleobase cation symporter 1 in Arabidopsis
    George S Mourad
    Department of Biology, Indiana University Purdue University Fort Wayne, Fort Wayne, IN 46805, USA
    FEBS Lett 586:1370-8. 2012
    ..This is in stark contrast to the canonical transport profiles determined for the well-characterized S. cerevisiae NCS1 proteins FUR4 (uracil transport) or FCY2 (adenine, guanine, and cytosine transport)...
  6. pmc Fluorescence monitoring of riboswitch transcription regulation using a dual molecular beacon assay
    Raja Chinnappan
    Department of Biology, Faculty of Sciences, RNA Group, Universite de Sherbrooke, Sherbrooke, Quebec J1K 2R1, Canada
    Nucleic Acids Res 41:e106. 2013
    ..This method is specific, reliable and could be applied at the high-throughput level for the identification of new potential antibiotics targeting any riboswitch-regulating gene expression at the mRNA level...
  7. pmc Substrate selectivity of YgfU, a uric acid transporter from Escherichia coli
    Konstantinos Papakostas
    Laboratory of Biological Chemistry, University of Ioannina Medical School, 45110 Ioannina, Greece
    J Biol Chem 287:15684-95. 2012
    ....
  8. pmc De novo pyrimidine nucleotide synthesis mainly occurs outside of plastids, but a previously undiscovered nucleobase importer provides substrates for the essential salvage pathway in Arabidopsis
    Sandra Witz
    Abteilung Pflanzenphysiologie, Fachbereich Biologie, Technische Universitat Kaiserslautern, D 67663 Kaiserslautern, Germany
    Plant Cell 24:1549-59. 2012
    ..Thus, a protein for the absolutely required import of pyrimidine nucleobases into plastids was identified...
  9. pmc Loss of heterozygosity of FCY2 leading to the development of flucytosine resistance in Candida tropicalis
    Yen Ning Chen
    Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan, Republic of China
    Antimicrob Agents Chemother 55:2506-14. 2011
    ..Subsequently, a combination of 5FC and another antifungal drug is applicable for treating infections of C. tropicalis...
  10. ncbi Fungal nucleobase transporters
    Areti Pantazopoulou
    Faculty of Biology, Department of Botany, University of Athens, Panepistimioupolis, Athens, Greece
    FEMS Microbiol Rev 31:657-75. 2007
    ..Apart from highlighting how a basic process such as nucleobase recognition and transport operates, this review intends to highlight features that might be applicable to antifungal pharmacology...
  11. ncbi Molecular genetic analysis of purine nucleobase transport in Leishmania major
    Diana Ortiz
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, OR 97239, USA
    Mol Microbiol 64:1228-43. 2007
    ..The phenotypes of these null mutants underscore the importance of purine nucleobase transporters in the Leishmania life cycle and pharmacology...
  12. ncbi Regulation of expression and kinetic modeling of substrate interactions of a uracil transporter in Aspergillus nidulans
    Sotiris Amillis
    Faculty of Biology, Department of Botany, University of Athens, Panepistimioupolis, Athens, Greece
    Mol Membr Biol 24:206-14. 2007
    ..This model, which rationalizes the unique specificity of FurD for uracil, is compared to and found to be very similar to analogous models for protozoan uracil transporters...
  13. pmc Control of basal extracellular adenosine concentration in rat cerebellum
    Mark J Wall
    Neuroscience Group, Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK
    J Physiol 582:137-51. 2007
    ..The increased spatial and temporal resolution of the purine biosensor measurements has revealed the complexity of the control of adenosine and purine tone in the cerebellum...
  14. ncbi Pyrimidine transport activities in trypanosomes
    Vivian Bellofatto
    Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey, Newark, NJ 07101, USA
    Trends Parasitol 23:187-9; discussion 190. 2007
    ..The work by Gudin et al. characterizes the dynamics of these transporters and points to further areas for future genetic and therapeutic experiments...
  15. ncbi Identification and expression analysis of twelve members of the nucleobase-ascorbate transporter (NAT) gene family in Arabidopsis thaliana
    Veronica G Maurino
    Botanisches Institut der Universitat zu Koln, Lehrstuhl II, Gyrhofstrasse 15, D 50931 Cologne, Germany
    Plant Cell Physiol 47:1381-93. 2006
    ....
  16. ncbi Various cytosine/adenine permease homologues are involved in the toxicity of 5-fluorocytosine in Saccharomyces cerevisiae
    John P Paluszynski
    Institut fur Molekulare Mikrobiologie und Biotechnologie, Westfalische Wilhelms Universitat Munster, Corrensstrasse 3, D 48149 Munster, Germany
    Yeast 23:707-15. 2006
    ..Among these, Tpn1, Fur4 and the one encoded by yOR071c were found to contribute significantly to 5-FC toxicity, thus revealing alternative entry routes for 5-FC via other cytosine/adenine permease homologues...
  17. ncbi Characterization of novel Na+-dependent nucleobase transport systems at the blood-testis barrier
    Ryo Kato
    Faculty of Pharmaceutical Sciences, Dept of Molecular Biopharmaceutics, Tokyo Univ of Science, 2641 Yamasaki, Noda, Chiba, 278 8510, Japan
    Am J Physiol Endocrinol Metab 290:E968-75. 2006
    ..In conclusion, it was suggested that there might be purine- or pyrimidine-selective nucleobase transporters in rat Sertoli cells...
  18. ncbi Comparative substrate recognition by bacterial and fungal purine transporters of the NAT/NCS2 family
    Sophia Goudela
    Department of Botany, University of Athens, Panepistimioupolis, Athens 15781, Greece
    Mol Membr Biol 22:263-75. 2005
    ..Both Xut1 and UapA recognize oxypurinol, but use different interactions reflected in a nearly 26-fold difference in their affinities for this drug, while YgfO interacts with this analogue very inefficiently...
  19. ncbi Cloning and functional characterization of two bacterial members of the NAT/NCS2 family in Escherichia coli
    Panayiota Karatza
    Laboratory of Biological Chemistry, University of Ioannina Medical School, Ioannina 45110, Greece
    Mol Membr Biol 22:251-61. 2005
    ....
  20. pmc Inactivation of the FCY2 gene encoding purine-cytosine permease promotes cross-resistance to flucytosine and fluconazole in Candida lusitaniae
    Florence Chapeland-Leclerc
    Laboratoire des Sciences Végétales, UFR des Sciences Pharmaceutiques et Biologiques, Universite Paris 5, 4 avenue de l Observatoire, 75006 Paris, France
    Antimicrob Agents Chemother 49:3101-8. 2005
    ..The results of this study provide molecular evidence that inactivation of the sole FCY2 gene promotes cross-resistance to the antifungal association 5FC-FLC in C. lusitaniae...
  21. ncbi Nucleoside and nucleobase transporters of primary human cardiac microvascular endothelial cells: characterization of a novel nucleobase transporter
    Derek B J Bone
    Department of Physiology and Pharmacology, University of Western Ontario, London, Ontario, Canada
    Am J Physiol Heart Circ Physiol 293:H3325-32. 2007
    ..Understanding the nucleoside and nucleobase transporter profiles in the vasculature will allow for further study into their roles in pathophysiological conditions such as hypoxia or ischemia...
  22. doi Cysteine-scanning analysis of putative helix XII in the YgfO xanthine permease: ILE-432 and ASN-430 are important
    Konstantinos Papakostas
    Laboratory of Biological Chemistry, University of Ioannina Medical School, 45110 Ioannina, Greece
    J Biol Chem 283:13666-78. 2008
    ..The findings suggest that Ile-432 and Asn-430 of helix XII are crucial for purine uptake and affinity, and Asn-430 is probably at the vicinity of the binding site...
  23. ncbi The Aspergillus nidulans FcyB cytosine-purine scavenger is highly expressed during germination and in reproductive compartments and is downregulated by endocytosis
    Anna Vlanti
    Faculty of Biology, Department of Botany, University of Athens, Panepistimioupolis, Athens 15781, Greece
    Mol Microbiol 68:959-77. 2008
    ..FcyB-GFP was also shown to be downregulated by endocytosis in response to ammonia or the presence of cytosine. The expression profile of FcyB supports that its main physiological role is cytosine-purine scavenging...
  24. ncbi Nucleoside/nucleobase transport and metabolism by microvascular endothelial cells isolated from ENT1-/- mice
    Derek B J Bone
    Department of Physiology and Pharmacology, University of Western Ontario, London, Canada
    Am J Physiol Heart Circ Physiol 299:H847-56. 2010
    ..Thus mouse MVECs from transgenic animals may prove to be a useful preclinical model for studies of the effects of purine metabolite modifiers on vascular function...
  25. pmc Purine substrate recognition by the nucleobase-ascorbate transporter signature motif in the YgfO xanthine permease: ASN-325 binds and ALA-323 senses substrate
    Ekaterini Georgopoulou
    Laboratory of Biological Chemistry, University of Ioannina Medical School, 45110 Ioannina, Greece
    J Biol Chem 285:19422-33. 2010
    ....
  26. doi Genetic prerequisites for additive or synergistic actions of 5-fluorocytosine and fluconazole in baker's yeast
    John P Paluszynski
    Institut fur Molekulare Mikrobiologie und Biotechnologie, Westfalische Wilhelms Universitat Munster, Corrensstr 3, D 48149 Munster, Germany
    Microbiology 154:3154-64. 2008
    ....
  27. pmc Identification and functional characterization of the first nucleobase transporter in mammals: implication in the species difference in the intestinal absorption mechanism of nucleobases and their analogs between higher primates and other mammals
    Syunsuke Yamamoto
    Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya 467 8603, Japan
    J Biol Chem 285:6522-31. 2010
    ..This may have a biological and evolutional meaning in the transport and metabolism of nucleobases. The present study provides novel insights into the specific transport and metabolism of nucleobases and their analogs for therapeutic use...
  28. pmc Role of intramembrane polar residues in the YgfO xanthine permease: HIS-31 and ASN-93 are crucial for affinity and specificity, and ASP-304 and GLU-272 are irreplaceable
    Ekaterini Karena
    Laboratory of Biological Chemistry, University of Ioannina Medical School, 45110 Ioannina, Greece
    J Biol Chem 284:24257-68. 2009
    ..Finally, one of the 17 assayable single-Cys mutants, Q258C, was sensitive to inactivation by N-ethylmaleimide. The findings suggest that polar residues important for the function of YgfO cluster in TMs 1, 3, 8 and 9a...
  29. pmc An acid-activated nucleobase transporter from Leishmania major
    Diana Ortiz
    Department of Molecular Microbiology and Immunology, Neuroscience Institute, Oregon Health and Science University, Portland, OR 97239, USA
    J Biol Chem 284:16164-9. 2009
    ..These observations imply that LmaNT3 and LmaNT4 are nucleobase/proton symporters...
  30. ncbi Characterization of nucleobase transport by mouse Sertoli cell line TM4
    Ryo Kato
    Department of Molecular Biopharmaceutics, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba, Japan
    Biol Pharm Bull 32:450-5. 2009
    ..These results suggested that there are distinct transport systems for purine and pyrimidine nucleobases in cells of mouse Sertoli cell line TM4...
  31. doi AtAzg1 and AtAzg2 comprise a novel family of purine transporters in Arabidopsis
    Tyler A Mansfield
    Department of Biology, Indiana University Purdue University Fort Wayne, Fort Wayne, IN 46805, USA
    FEBS Lett 583:481-6. 2009
    ..Radio-labeled purine uptake experiments in yeast and in planta confirm the function of AtAzg1 and AtAzg2 as plant adenine-guanine transporters...
  32. pmc Characterization of cytokinin and adenine transport in Arabidopsis cell cultures
    Anna Cedzich
    Center for Plant Molecular Biology, University of Tuebingen, D 72071 Tuebingen, Germany
    Plant Physiol 148:1857-67. 2008
    ....
  33. doi Mutational analysis and modeling reveal functionally critical residues in transmembrane segments 1 and 3 of the UapA transporter
    Sotiris Amillis
    Department of Botany, Faculty of Biology, University of Athens, Panepistimioupolis, Athens 15781, Greece
    J Mol Biol 411:567-80. 2011
    ..Our results are in agreement with a topological model of UapA built on the recently published structure of UraA, a bacterial homolog of UapA...
  34. doi A purine-selective nucleobase/nucleoside transporter in PK15NTD cells
    Kazi Mirajul Hoque
    Department of Medicine, The Johns Hopkins University, Baltimore, MD 21205 2109, USA
    Am J Physiol Regul Integr Comp Physiol 294:R1988-95. 2008
    ..This transporter is potentially important for transporting naturally occurring purines and purine analog drugs into cells...
  35. doi The nucleobase-ascorbate transporter (NAT) family: genomics, evolution, structure-function relationships and physiological role
    Christos Gournas
    Faculty of Biology, Department of Botany, University of Athens, Panepistimioupolis, Athens, Greece
    Mol Biosyst 4:404-16. 2008
    ..Finally, we also discuss how modelling NAT-purine interactions might constitute a step towards the use of NAT proteins as specific gateways for targeting pathogenic microbes...
  36. ncbi Identification of the first pyrimidine nucleobase transporter in Leishmania: similarities with the Trypanosoma brucei U1 transporter and antileishmanial activity of uracil analogues
    I G Papageorgiou
    Department of Biochemistry, Hellenic Pasteur Institute, 115 21 Athens, Greece
    Parasitology 130:275-83. 2005
    ..Overall, the results show that, like purine nucleobase transport, pyrimidine nucleobase transport function is very similar in L. major and T. brucei insect forms...
  37. ncbi The PAUSE software for analysis of translational control over protein targeting: application to E. nidulans membrane proteins
    P Dessen
    Service de Bioinformatique UMS825 CNRS SC13 INSERM, 7 Rue Guy Moquet BP8, 94801, Villejuif, France
    Gene 244:89-96. 2000
    ..Thus, these specific pauses might reflect a translational control over membrane protein targeting or early recognition ('+45 pause'), and over insertion or folding ('+70 pause')...
  38. ncbi Evidence for a dynamic role for proline376 in the purine-cytosine permease of Saccharomyces cerevisiae
    T Ferreira
    Institut de Biochimie et Géntique Celluaires de Centre National de la Recherche Scientifique, UPR 9026, Bordeaux, France
    Eur J Biochem 263:57-64. 1999
    ..They also show that the P376 residue is part of an unusual secondary structure, probably a beta-turn motif, which must play a crucial dynamic role in the translocation process...
  39. ncbi Screening of an intragenic second-site suppressor of purine-cytosine permease from Saccharomyces cerevisiae. Possible role of Ser272 in the base translocation process
    T Ferreira
    Institut de Biochimie et Génétique Cellulaires, Centre National de la Recherche Scientifique, Bordeaux, France
    Eur J Biochem 260:22-30. 1999
    ..The location and the observed decrease in the turnover of the carrier observed with the S272 L change lead us to propose that S272 could be part of a hydrophilic pore involved in the translocation of the base and/or the proton...
  40. ncbi Characterization of the Saccharomyces cerevisiae cytosine transporter using energizable plasma membrane vesicles
    B Pinson
    Institut de Biochimie et Génétique Cellulaires du Centre National de la Recherche Scientifique, Bordeaux, France
    J Biol Chem 272:28918-24. 1997
    ..Finally, no specific role of K+ ions on cytosine transport could be demonstrated in this system...
  41. ncbi Functional analysis of mutated purine-cytosine permease from Saccharomyces cerevisiae. A possible role of the hydrophilic segment 371-377 in the active carrier conformation
    T Ferreira
    Institut de Biochimie et Génétique Cellulaires du Centre National de la Recherche Scientifique, UPR 9026, 1 rue Camille Saint Saens, F 33077 Bordeaux cedex, France
    J Biol Chem 272:9697-702. 1997
    ..This result suggests that the 371-377 segment might play a key role in the proper three-dimensional structure of the active purine-cytosine permease...
  42. ncbi In vivo phosphorylation of the purine/cytosine permease from the plasma membrane of the yeast Saccharomyces cerevisiae
    B Pinson
    Institut de Biochimie et Génétique Cellulaires du Centre National de la Recherche Scientifique, Bordeaux, France
    Eur J Biochem 239:439-44. 1996
    ..By means of thermosensitive secretory-pathway-mutant strains, we demonstrated that purine/cytosine permease phosphorylation occurred either between the Golgi apparatus and the plasma membrane or in the plasma membrane itself...
  43. ncbi Detection of purine cytosine permease of S. cerevisiae: use of antibodies against a synthetic peptide corresponding to a predicted sequence in the N-terminal domain of the protein
    X Grandier-Vazeille
    Institut de Biochimie Cellulaire, Centre National de la Recherche Scientifique, Bordeaux, France
    Biochem Biophys Res Commun 197:372-9. 1993
    ..These results demonstrate the specificity of the antisera and also suggest that the 45 kDa and 50 kDa proteins are both products of the FCY2 gene...
  44. ncbi A purine permease in Candida glabrata
    S Sen Gupta
    Yale Universtiy School of Medicine, Department of Genetics, New Haven, Connecticut 06510 8005
    FEMS Microbiol Lett 126:93-6. 1995
    ..glabrata. This differs from the purine cytosine permeases in Saccharomyces cerevisiae and Candida albicans which transport adenine, cytosine, guanine and hypoxanthine...
  45. ncbi Genetic and molecular characterization of a gene encoding a wide specificity purine permease of Aspergillus nidulans reveals a novel family of transporters conserved in prokaryotes and eukaryotes
    G Diallinas
    Institut de Genetique et Microbiologie, Unité Associé au Centre National de la Recherche Scientifique 1354, Universite de Paris Sud, Centre d Orsay, France
    J Biol Chem 270:8610-22. 1995
    ..Comparisons of the sequences and hydropathy profiles of members of this novel family of transporters yield insights into their structure, functionally important residues, and possible evolutionary relationships...
  46. ncbi Photoaffinity labelling of the purine-cytosine permease of Saccharomyces cerevisiae
    M C Chirio
    Institut de Biochimie Cellulaire Centre, National de la Recherche Scientifique, Bordeaux, France
    Eur J Biochem 194:293-9. 1990
    ..Therefore this polypeptide corresponds to the purine-cytosine permease...
  47. ncbi The purine-cytosine permease gene of Saccharomyces cerevisiae: primary structure and deduced protein sequence of the FCY2 gene product
    E Weber
    I B M C du C N R S, Strasbourg, France
    Mol Microbiol 4:585-96. 1990
    ..Expression of the FCY2 gene product requires a functional secretory pathway and is reduced in mnn9, a mutant strain deficient in outer chain glycosylation. The FCY2 gene was mapped on the right arm of chromosome V close to the HIS1 gene...
  48. ncbi In vivo and in vitro studies of the purine-cytosine permease of Saccharomyces cerevisiae. Functional analysis of a mutant with an altered apparent transport constant of uptake
    D Brethes
    Institut de Biochimie Cellulaire et de Neurochimie, Centre National de la Recherche Scientifique, Bordeaux, France
    Eur J Biochem 204:699-704. 1992
    ..Therefore, we conclude that modification in the Ktapp of uptake in the strain carrying fcy2-21 allele is merely due to a modification of the binding ability of the permease for its ligands...
  49. ncbi Purine-cytosine permease of Saccharomyces cerevisiae. Effect of external pH on nucleobase uptake and binding
    D Brethes
    Institut de Biochimie Cellulaire du Centre National de la Recherche Scientifique ERS 0022, Bordeaux, France
    Eur J Biochem 210:785-91. 1992
    ..9). The pK of this group remained similar to the value obtained with pAB4 when hypoxanthine was bound. From these data, it is proposed that the binding of hypoxanthine and H+ is a random process...
  50. ncbi Determination of a specific region of the purine-cytosine permease involved in the recognition of its substrates
    J C Bloch
    URA GEM, CNRS, Strasbourg, France
    Mol Microbiol 6:2989-97. 1992
    ..In particular, amino acids at position 374 and 377 modulate the affinity of the permease towards its substrates. In the wild-type, when asparagine is present at both of these positions, the lowest Kmapp values are found...
  51. ncbi Fluorocytosine causes uncoupled dissipation of the proton gradient and behaves as an imperfect substrate of the yeast cytosine permease
    P Hopkins
    Department of Biochemistry and Applied Molecular Biology, University of Manchester, U K
    Yeast 8:1053-64. 1992
    ..A possible mechanism of this novel phenomenon is outlined...
  52. ncbi Construction of efficient centromeric, multicopy and expression vectors for the yeast Kluyveromyces marxianus using homologous elements and the promoter of a purine-cytosine-like permease
    M M Ball
    Laboratorio Organizacion y Expresión del Gen, Dpto de Biologia, Facultad de Ciencas, Merida, Venezuela
    J Mol Microbiol Biotechnol 1:347-53. 1999
    ..This promoter appears to be constitutive in various conditions tested. Its five transcription start sites have been mapped, and a derivative expression vector for K. marxianus has been constructed...
  53. ncbi Functional production of mammalian concentrative nucleoside transporters in Saccharomyces cerevisiae
    M F Vickers
    University of Alberta, and Cross Cancer Institute, Edmonton, Canada
    Mol Membr Biol 18:73-9. 2001
    ..This work represents the development of a new model system for the functional production of recombinant nucleoside transporters of the CNT family of membrane proteins...
  54. pmc New plasmid system to select for Saccharomyces cerevisiae purine-cytosine permease affinity mutants
    R Wagner
    Laboratoire de Microbiologie et de Genetique, ESA 7010 Université Louis Pasteur CNRS, Institut de Botanique, F 67083 Strasbourg cedex, France
    J Bacteriol 183:4386-8. 2001
    ..In this study, we developed a plasmid-based system that generated a number of affinity-mutated alleles, enabling us to define new amino acids critical for permease function...
  55. ncbi Trypanosoma brucei: expression of multiple purine transporters prevents the development of allopurinol resistance
    Manal J Natto
    Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, Joseph Black Building, Glasgow G12 8QQ, UK
    Exp Parasitol 109:80-6. 2005
    ..This study shows that transport-related resistance to purine antimetabolites is not easily induced in Trypanosoma spp. as long as uptake is mediated by multiple transporters...
  56. ncbi Selective transport of a new class of purine antimetabolites by the protozoan parasite Trypanosoma brucei
    L J M Wallace
    Institute of Biomedical and Life Sciences, Division of Infection and Immunity, University of Glasgow, Glasgow, UK
    Nucleosides Nucleotides Nucleic Acids 23:1441-4. 2004
    ..We show that these compounds display a remarkable selectivity for the parasite's transporters. The adenine analogue showed greater trypanocidal activity than the hypoxanthine or guanine analogues in vitro...
  57. pmc Nucleoside and nucleobase transporters in parasitic protozoa
    Scott M Landfear
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, Oregon 97239 3098, USA
    Eukaryot Cell 3:245-54. 2004
  58. ncbi Transcription of purine transporter genes is activated during the isotropic growth phase of Aspergillus nidulans conidia
    Sotiris Amillis
    Faculty of Biology, Department of Botany, University of Athens, Panepistimioupolis, Athens 15781, Greece
    Mol Microbiol 52:205-16. 2004
    ..Similar results have been found in studies on the expression of other transporters in A. nidulans, suggesting that global expression of transporters might operate as a general system for sensing solute availability...
  59. ncbi Functional expression and characterization of a purine nucleobase transporter gene from Leishmania major
    Marco A Sanchez
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, OR 97201, USA
    Mol Membr Biol 21:11-8. 2004
    ..5, 8.5 +/- 0.6, 8.5 +/- 1.1, and 8.8 +/- 4.0 microM, respectively. LmaNT3 is, thus, the first member of the ENT family identified in any organism that functions as a nucleobase rather than nucleoside or nucleoside/nucleobase transporter...
  60. ncbi Ammonium-induced internalisation of UapC, the general purine permease from Aspergillus nidulans
    Javier Valdez-Taubas
    Departamento de Quimica Biologica, Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, Ciudad Universitaria, 5000 Cordoba, Argentina
    Fungal Genet Biol 41:42-51. 2004
    ..These results provide further support for the occurrence of endocytosis and the lysosomal-endosomal function of the vacuolar compartment in A. nidulans...
  61. ncbi Molecular and functional characterization of the first nucleobase transporter gene from African trypanosomes
    Cristina Henriques
    Department of Molecular Microbiology and Immunology, Oregon Health and Science University, 3181 S W Sam Jackson Park Road, Portland, OR 97201, USA
    Mol Biochem Parasitol 130:101-10. 2003
    ..1 mRNA is significantly more abundant in insect stage procyclic forms than in mammalian stage bloodstream forms, and the TbNT8.1 permease thus may represent a major route for purine nucleobase uptake in procyclic trypanosomes...
  62. ncbi Identification and characterisation of high affinity nucleoside and nucleobase transporters in Toxoplasma gondii
    Harry P de Koning
    Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, Joseph Black Building, Glasgow G12 8QQ, UK
    Int J Parasitol 33:821-31. 2003
    ..gondii...
  63. ncbi Transport of cytokinins mediated by purine transporters of the PUP family expressed in phloem, hydathodes, and pollen of Arabidopsis
    Lukas Bürkle
    Plant Physiology, Zentrum für Molekularbiologie der Pflanzen, Auf der Morgenstelle 1, D 72076 Tubingen, Germany
    Plant J 34:13-26. 2003
    ..In summary, three closely related PUPs are differentially expressed in Arabidopsis and at least two PUPs have properties similar to the adenine and cytokinin transport system identified in Arabidopsis cell cultures...
  64. pmc Genome-wide identification of nodule-specific transcripts in the model legume Medicago truncatula
    Maria Fedorova
    Department of Agronomy and Plant Genetics, 1991 Upper Bedford Circle, University of Minnesota, St Paul, MN 55108, USA
    Plant Physiol 130:519-37. 2002
    ..Nodule-enhanced expression was confirmed experimentally for the TCs composed of five or more ESTs, whereas the results for those TCs containing fewer ESTs were variable...
  65. ncbi A Leishmania major nucleobase transporter responsible for allopurinol uptake is a functional homolog of the Trypanosoma brucei H2 transporter
    Mohammed I Al-Salabi
    Institute of Biomedical and Life Sciences, Division of Infection and Immunity, Joseph Black Building, University of Glasgow, Glasgow, United Kingdom
    Mol Pharmacol 63:814-20. 2003
    ..It is proposed that the architecture of their respective binding sites is very similar and that LmNBT1 can be named a functional homolog of TbH2...
  66. ncbi Na+ gradient-dependent transport of hypoxanthine by calf intestinal brush border membrane vesicles
    A Theisinger
    Institute of Veterinary Physiology, University of Zurich, Winterthurerstrasse 260, CH 8057 Zurich, Switzerland
    J Comp Physiol B 173:165-70. 2003
    ..These findings represent the first demonstration of active Na(+) gradient-dependent nucleobase transport in intestinal brush border membrane vesicles...
  67. pmc Structure and molecular mechanism of a nucleobase-cation-symport-1 family transporter
    Simone Weyand
    Membrane Protein Laboratory, Diamond Light Source Limited, Harwell Science and Innovation Campus, Chilton, Didcot, Oxfordshire OX11 0DE, UK
    Science 322:709-13. 2008
    ..The reciprocal opening and closing of these cavities is synchronized by the inverted repeat helices 3 and 8, providing the structural basis of the alternating access model for membrane transport...
  68. ncbi Cysteine-scanning analysis of the nucleobase-ascorbate transporter signature motif in YgfO permease of Escherichia coli: Gln-324 and Asn-325 are essential, and Ile-329-Val-339 form an alpha-helix
    Panayiota Karatza
    Laboratory of Biological Chemistry, University of Ioannina Medical School, 45110 Ioannina, Greece
    J Biol Chem 281:39881-90. 2006
    ..4 mM, positions 323-329 are highly sensitive, with IC(50) values of 15-80 microM, and sensitivity of positions 330-340 follows a periodicity, with mutants sensitive to inactivation clustering on one face of an alpha-helix...
  69. pmc Molecular mechanisms of primary resistance to flucytosine in Candida albicans
    William W Hope
    Immunocompromised Host Section POB, NCI, NIH CRC, Room 1 5700, 10 Center Dr, MSC 1100, Bethesda, MD 20892 1100, USA
    Antimicrob Agents Chemother 48:4377-86. 2004
    ..A single resistant isolate, lacking the above polymorphism in FUR1, has a homozygous polymorphism in FCA1 that results in a glycine-to-aspartate substitution at position 28 in cytosine deaminase...
  70. pmc Uptake of purines in Plasmodium falciparum-infected human erythrocytes is mostly mediated by the human equilibrative nucleoside transporter and the human facilitative nucleobase transporter
    Neils B Quashie
    Institute of Biomedical and Life Sciences, Division of Infection and Immunity, Glasgow Biomedical Research Centre, University of Glasgow, Glasgow G128TA, UK
    Malar J 9:36. 2010
    ..Using the appropriate transporter inhibitors, the role of each of these salvage pathways to the overall purine transport in intraerythrocytic Plasmodium falciparum was systematically investigated...

Research Grants61

  1. Purine Nucleoside/Nucleobase Transporters in Leishmania
    Scott M Landfear; Fiscal Year: 2010
    ..The essential roles that purine nucleoside/nucleobase transporters play in parasite viability further suggests selective inhibition of these transporters could be exploited to develop novel drugs against parasitic diseases. ..
  2. Functional Characterization of Leishmania Transporters
    Scott Landfear; Fiscal Year: 2005
    ..These studies will initiate the molecular analysis of amino acid transporters in Leishmania. ..
  3. Functional Characterization of Leishmania Transporters
    Scott Landfear; Fiscal Year: 2007
    ..Overall, this proposal will provide a detailed biochemical and genetic dissection of glucose transporter function and the essential role of these permeases in the parasite life cycle. ..
  4. Nucleoside/Nucleobase Transporters in Leishmania major
    Scott Landfear; Fiscal Year: 2007
    ..We will determine the ligand specificity and affinities for LmaNT4 and evaluate LmaNT4 expression throughout the L. major life cycle. ..
  5. Development of Assay for High Throughput Screen of Parasite Glucose Transporter
    Scott M Landfear; Fiscal Year: 2010
    ..Since glucose is an essential nutrient for these parasites, such compounds would kill the parasite and could act as novel drugs for treatment of the diseases they cause. ..
  6. ANALYSIS OF TRYPANOSOME MRNA SYNTHESIS BY GENE TRANSFER
    Vivian Bellofatto; Fiscal Year: 2010
    ..The goal of our biomedical work is to define unique features of trypanosome biology and then to exploit these traits for the design of anti-parasitic chemotherapies. ..
  7. ANALYSIS OF TRYPANOSOME MRNA SYNTHESIS BY GENE TRANSFER
    Vivian Bellofatto; Fiscal Year: 2007
    ..brucei chromosome 2. We have recently developed a preinitiation complex formation assay, a Chromatin Immunoprecipation assay and a yeast two-hybrid system that are pertinent tools for our transcriptional dissections. ..
  8. Regulation of mRNA Turnover in Trypanosomes
    Vivian Bellofatto; Fiscal Year: 2007
    ..We have recently developed a cell-free system that is competent for both of these enzymatic activities. This work is a key first step in our proposed experimental plan. [unreadable] [unreadable]..
  9. Functional Characterization of Leishmania Transporters
    Scott Landfear; Fiscal Year: 2006
    ..Overall, this proposal will provide a detailed biochemical and genetic dissection of glucose transporter function and the essential role of these permeases in the parasite life cycle. ..
  10. Nucleoside/Nucleobase Transporters in Leishmania major
    Scott Landfear; Fiscal Year: 2006
    ..We will determine the ligand specificity and affinities for LmaNT4 and evaluate LmaNT4 expression throughout the L. major life cycle. ..
  11. ANALYSIS OF TRYPANOSOME MRNA SYNTHESIS BY GENE TRANSFER
    Vivian Bellofatto; Fiscal Year: 2006
    ..brucei chromosome 2. We have recently developed a preinitiation complex formation assay, a Chromatin Immunoprecipation assay and a yeast two-hybrid system that are pertinent tools for our transcriptional dissections. ..
  12. Regulation of mRNA Turnover in Trypanosomes
    Vivian Bellofatto; Fiscal Year: 2006
    ..We have recently developed a cell-free system that is competent for both of these enzymatic activities. This work is a key first step in our proposed experimental plan. [unreadable] [unreadable]..
  13. Nucleoside/Nucleobase Transporters in Leishmania major
    Scott Landfear; Fiscal Year: 2005
    ..We will determine the ligand specificity and affinities for LmaNT4 and evaluate LmaNT4 expression throughout the L. major life cycle. ..
  14. ANALYSIS OF TRYPANOSOME MRNA SYNTHESIS BY GENE TRANSFER
    Vivian Bellofatto; Fiscal Year: 2005
    ..brucei chromosome 2. We have recently developed a preinitiation complex formation assay, a Chromatin Immunoprecipation assay and a yeast two-hybrid system that are pertinent tools for our transcriptional dissections. ..
  15. Regulation of mRNA Turnover in Trypanosomes
    Vivian Bellofatto; Fiscal Year: 2005
    ..We have recently developed a cell-free system that is competent for both of these enzymatic activities. This work is a key first step in our proposed experimental plan. ..
  16. Nucleoside/Nucleobase Transporters in Leishmania major
    Scott Landfear; Fiscal Year: 2004
    ..We will determine the ligand specificity and affinities for LmaNT4 and evaluate LmaNT4 expression throughout the L. major life cycle. ..
  17. Functional Characterization of Leishmania Transporters
    Scott Landfear; Fiscal Year: 2004
    ..These studies will initiate the molecular analysis of amino acid transporters in Leishmania. ..
  18. Regulation of mRNA Turnover in Trypanosomes
    Vivian Bellofatto; Fiscal Year: 2004
    ..We have recently developed a cell-free system that is competent for both of these enzymatic activities. This work is a key first step in our proposed experimental plan. ..
  19. Functional Characterization of Leishmania Transporters
    Scott M Landfear; Fiscal Year: 2010
    ..Overall, this proposal will provide a detailed biochemical and genetic dissection of glucose transporter function and the essential role of these permeases in the parasite life cycle. ..
  20. ANALYSIS OF TRYPANOSOME MRNA SYNTHESIS BY GENE TRANSFER
    Vivian Bellofatto; Fiscal Year: 2009
    ..The goal of our biomedical work is to define unique features of trypanosome biology and then to exploit these traits for the design of anti-parasitic chemotherapies. ..
  21. Development of Assay for High Throughput Screen of Parasite Glucose Transporter
    Scott Landfear; Fiscal Year: 2009
    ..Since glucose is an essential nutrient for these parasites, such compounds would kill the parasite and could act as novel drugs for treatment of the diseases they cause. ..
  22. Functional Characterization of Leishmania Transporters
    Scott Landfear; Fiscal Year: 2009
    ..Overall, this proposal will provide a detailed biochemical and genetic dissection of glucose transporter function and the essential role of these permeases in the parasite life cycle. ..
  23. Development of Assay for High Throughput Screen of Parasite Glucose Transporter
    Scott Landfear; Fiscal Year: 2008
    ..Since glucose is an essential nutrient for these parasites, such compounds would kill the parasite and could act as novel drugs for treatment of the diseases they cause. ..
  24. INTERACTIONS AT THE MICROBE/HOST INTERFACE
    Scott Landfear; Fiscal Year: 2008
    ....
  25. Nucleoside/Nucleobase Transporters in Leishmania major
    Scott Landfear; Fiscal Year: 2008
    ..We will determine the ligand specificity and affinities for LmaNT4 and evaluate LmaNT4 expression throughout the L. major life cycle. ..
  26. ANALYSIS OF TRYPANOSOME MRNA SYNTHESIS BY GENE TRANSFER
    Vivian Bellofatto; Fiscal Year: 2008
    ..brucei chromosome 2. We have recently developed a preinitiation complex formation assay, a Chromatin Immunoprecipation assay and a yeast two-hybrid system that are pertinent tools for our transcriptional dissections. ..
  27. Regulation of mRNA Turnover in Trypanosomes
    Vivian Bellofatto; Fiscal Year: 2008
    ..We have recently developed a cell-free system that is competent for both of these enzymatic activities. This work is a key first step in our proposed experimental plan. [unreadable] [unreadable]..
  28. INTERACTIONS AT THE MICROBE/HOST INTERFACE
    Scott Landfear; Fiscal Year: 2007
    ....
  29. ANALYSIS OF TRYPANOSOME MRNA SYNTHESIS BY GENE TRANSFER
    Vivian Bellofatto; Fiscal Year: 2004
    ..brucei chromosome 2. We have recently developed a preinitiation complex formation assay, a Chromatin Immunoprecipation assay and a yeast two-hybrid system that are pertinent tools for our transcriptional dissections. ..
  30. NUCLEOSIDE TRANSPORTERS IN LEISHMANIA AND TRYPANOSOMES
    Scott Landfear; Fiscal Year: 2003
    ..donovani and oocytes, to determine whether either transporter recognizes melarsoprol or pentamidine, and to discover whether three truncated versions of TbNT1 and TbNT2 encode functional nucleoside transporters. ..
  31. Functional Characterization of Leishmania Transporters
    Scott Landfear; Fiscal Year: 2003
    ..These studies will initiate the molecular analysis of amino acid transporters in Leishmania. ..
  32. STAGE-SPECIFIC GENES IN LEISHMANIA ENRIETTII
    Scott Landfear; Fiscal Year: 1991
    ....
  33. FUNCTIONAL CHARACTERIZATION OF LEISHMANIA TRANSPORTER
    Scott Landfear; Fiscal Year: 1999
    ..These experiments will initiate a detailed molecular study of these biochemically and pharmacologically important transporters in these purine auxotrophic parasites. ..
  34. TRYPANOSOME MRNA SYNTHESIS BY GENE TRANSFER
    Vivian Bellofatto; Fiscal Year: 1999
    ..These analyses, which will include gene cloning and protein function studies, will help identify the specific mechanisms that trypanosomes employ to accomplish this fundamental cellular process. ..
  35. NUCLEOSIDE TRANSPORTERS IN LEISHMANIA AND TRYPANOSOMES
    Scott Landfear; Fiscal Year: 1999
    ..donovani and oocytes, to determine whether either transporter recognizes melarsoprol or pentamidine, and to discover whether three truncated versions of TbNT1 and TbNT2 encode functional nucleoside transporters. ..
  36. ANALYSIS OF TRYPANOSOME MRNA SYNTHESIS BY GENE TRANSFER
    Vivian Bellofatto; Fiscal Year: 1993
    ....
  37. STAGE-SPECIFIC GENES IN LEISHMANIA ENRIETTII
    Scott Landfear; Fiscal Year: 1990
    ....
  38. TRYPANOSOME MRNA SYNTHESIS BY GENE TRANSFER
    Vivian Bellofatto; Fiscal Year: 2003
    ..These analyses, which will include gene cloning and protein function studies, will help identify the specific mechanisms that trypanosomes employ to accomplish this fundamental cellular process. ..
  39. TRYPANOSOME MRNA SYNTHESIS BY GENE TRANSFER
    Vivian Bellofatto; Fiscal Year: 2002
    ..These analyses, which will include gene cloning and protein function studies, will help identify the specific mechanisms that trypanosomes employ to accomplish this fundamental cellular process. ..
  40. NUCLEOSIDE TRANSPORTERS IN LEISHMANIA AND TRYPANOSOMES
    Scott Landfear; Fiscal Year: 2002
    ..donovani and oocytes, to determine whether either transporter recognizes melarsoprol or pentamidine, and to discover whether three truncated versions of TbNT1 and TbNT2 encode functional nucleoside transporters. ..
  41. Functional Characterization of Leishmania Transporters
    Scott Landfear; Fiscal Year: 2002
    ..These studies will initiate the molecular analysis of amino acid transporters in Leishmania. ..
  42. TRYPANOSOME MRNA SYNTHESIS BY GENE TRANSFER
    Vivian Bellofatto; Fiscal Year: 2002
    ..These analyses, which will include gene cloning and protein function studies, will help identify the specific mechanisms that trypanosomes employ to accomplish this fundamental cellular process. ..
  43. Functional Characterization of Leishmania Transporters
    Scott Landfear; Fiscal Year: 2001
    ..These studies will initiate the molecular analysis of amino acid transporters in Leishmania. ..
  44. NUCLEOSIDE TRANSPORTERS IN LEISHMANIA AND TRYPANOSOMES
    Scott Landfear; Fiscal Year: 2001
    ..donovani and oocytes, to determine whether either transporter recognizes melarsoprol or pentamidine, and to discover whether three truncated versions of TbNT1 and TbNT2 encode functional nucleoside transporters. ..
  45. TRYPANOSOME MRNA SYNTHESIS BY GENE TRANSFER
    Vivian Bellofatto; Fiscal Year: 2001
    ..These analyses, which will include gene cloning and protein function studies, will help identify the specific mechanisms that trypanosomes employ to accomplish this fundamental cellular process. ..
  46. FUNCTIONAL CHARACTERIZATION OF LEISHMANIA TRANSPORTER
    Scott Landfear; Fiscal Year: 2000
    ..These experiments will initiate a detailed molecular study of these biochemically and pharmacologically important transporters in these purine auxotrophic parasites. ..
  47. NUCLEOSIDE TRANSPORTERS IN LEISHMANIA AND TRYPANOSOMES
    Scott Landfear; Fiscal Year: 2000
    ..donovani and oocytes, to determine whether either transporter recognizes melarsoprol or pentamidine, and to discover whether three truncated versions of TbNT1 and TbNT2 encode functional nucleoside transporters. ..
  48. TRYPANOSOME MRNA SYNTHESIS BY GENE TRANSFER
    Vivian Bellofatto; Fiscal Year: 2000
    ..These analyses, which will include gene cloning and protein function studies, will help identify the specific mechanisms that trypanosomes employ to accomplish this fundamental cellular process. ..
  49. STAGE-SPECIFIC GENES IN LEISHMANIA ENRIETTII
    Scott Landfear; Fiscal Year: 1993
    ....
  50. STAGE-SPECIFIC GENES IN LEISHMANIA ENRIETTII
    Scott Landfear; Fiscal Year: 1992
    ....