Genomes and Genes
f box proteins
Summary: A family of proteins that share the F-BOX MOTIF and are involved in protein-protein interactions. They play an important role in process of protein ubiquition by associating with a variety of substrates and then associating into SCF UBIQUITIN LIGASE complexes. They are held in the ubiquitin-ligase complex via binding to SKP DOMAIN PROTEINS.
Publications246 found, 100 shown here
- The Fbw7 tumor suppressor regulates glycogen synthase kinase 3 phosphorylation-dependent c-Myc protein degradationMarkus Welcker
Division of Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
Proc Natl Acad Sci U S A 101:9085-90. 2004..Because Fbw7 mediates the degradation of cyclin E, Notch, and c-Jun, as well as c-Myc, the loss of Fbw7 is likely to elicit profound effects on cell proliferation during tumorigenesis...
- Systematic analysis and nomenclature of mammalian F-box proteinsJianping Jin
Genes Dev 18:2573-80. 2004
- Phosphorylation-dependent ubiquitination of cyclin E by the SCFFbw7 ubiquitin ligaseD M Koepp
Department of Biochemistry and Molecular Biology, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX, 77030, USA
Science 294:173-7. 2001..Multiple F-box proteins contribute to cyclin E stability in yeast, suggesting an overlap in SCF E3 ligase specificity that allows combinatorial control of cyclin E degradation...
- The SCFFBW7 ubiquitin ligase complex as a tumor suppressor in T cell leukemiaBenjamin J Thompson
Department of Pathology, New York University School of Medicine, New York, NY 10016, USA
J Exp Med 204:1825-35. 2007..Our data suggest that FBW7 is a novel tumor suppressor in T cell leukemia, and implicate the loss of FBW7 function as a potential mechanism of drug resistance in T-ALL...
- Plant development is regulated by a family of auxin receptor F box proteinsNihal Dharmasiri
Department of Biology, Indiana University, Bloomington, Indiana 47405, USA
Dev Cell 9:109-19. 2005..Here we show that three additional F box proteins, called AFB1, 2, and 3, also regulate auxin response...
- SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-boxC Bai
Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030, USA
Cell 86:263-74. 1996..Different skp1 mutants arrest cells in either G1 or G2, suggesting a connection between regulation of proteolysis in different stages of the cycle...
- Structure of a Fbw7-Skp1-cyclin E complex: multisite-phosphorylated substrate recognition by SCF ubiquitin ligasesBing Hao
Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
Mol Cell 26:131-43. 2007....
- FBW7 mutations in leukemic cells mediate NOTCH pathway activation and resistance to gamma-secretase inhibitorsJennifer O'Neil
Department of Pediatric Oncology, Dana Farber Cancer Institute, Boston, MA 02115, USA
J Exp Med 204:1813-24. 2007....
- FBXW7/hCDC4 is a general tumor suppressor in human cancerShahab Akhoondi
Cancer Center Karolinska, Karolinska Hospital, Stockholm, Sweden
Cancer Res 67:9006-12. 2007..Our study is the first comprehensive screen of FBXW7 mutations in various human malignancies and shows that FBXW7 is a general tumor suppressor in human cancer...
- Histone demethylation by a family of JmjC domain-containing proteinsYu Ichi Tsukada
Howard Hughes Medical Institute, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
Nature 439:811-6. 2006..Thus, we identify the JmjC domain as a novel demethylase signature motif and uncover a protein demethylation mechanism that is conserved from yeast to human...
- Multisite phosphorylation by Cdk2 and GSK3 controls cyclin E degradationMarkus Welcker
Divisions of Clinical Research and Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
Mol Cell 12:381-92. 2003..Thus, cyclin E turnover is controlled by multiple biological inputs and cannot be understood in terms of autophosphorylation alone...
- A nucleolar isoform of the Fbw7 ubiquitin ligase regulates c-Myc and cell sizeMarkus Welcker
Divisions of Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
Curr Biol 14:1852-7. 2004..We suggest that interactions between c-Myc and Fbw7gamma within the nucleolus regulate c-Myc's growth-promoting function and that c-Myc activation is likely to be an important oncogenic consequence of Fbw7 loss in cancers...
- FBW7 ubiquitin ligase: a tumour suppressor at the crossroads of cell division, growth and differentiationMarkus Welcker
Division of Human Biology, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, Washington 98109, USA
Nat Rev Cancer 8:83-93. 2008..This Review focuses on structural and functional aspects of FBW7 and its role in the development of cancer...
- Phosphorylation-dependent ubiquitination of cyclin D1 by the SCF(FBX4-alphaB crystallin) complexDouglas I Lin
The Leonard and Madlyn Abramson Family Cancer Research Institute and Cancer Center, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
Mol Cell 24:355-66. 2006..We conclude that SCF(FBX4-alphaB crystallin) is an E3 ubiquitin ligase that promotes ubiquitin-dependent degradation of Thr286-phosphorylated cyclin D1...
- Inactivation of hCDC4 can cause chromosomal instabilityHarith Rajagopalan
The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21231, USA
Nature 428:77-81. 2004..Our data suggest that chromosomal instability is caused by specific genetic alterations in a large fraction of human cancers and can occur before malignant conversion...
- EIN3-dependent regulation of plant ethylene hormone signaling by two arabidopsis F box proteins: EBF1 and EBF2Thomas Potuschak
Institut de Biologie Moleculaire des Plantes du CNRS, 12 rue du Général Zimmer, 67084 Cedex, Strasbourg, France
Cell 115:679-89. 2003..Here, we identify two Arabidopsis F box proteins called EBF1 and EBF2 that interact physically with EIN3/EIL transcription factors...
- WD40 repeat propellers define a ubiquitin-binding domain that regulates turnover of F box proteinsNatasha Pashkova
Department of Molecular Physiology and Biophysics, University of Iowa, Iowa City, IA 52242, USA
Mol Cell 40:433-43. 2010..Collectively, our results reveal a molecular mechanism that may account for how ubiquitin controls a broad spectrum of cellular activities...
- Phosphorylation-dependent degradation of c-Myc is mediated by the F-box protein Fbw7Masayoshi Yada
Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Maidashi, Higashi ku, Fukuoka, Fukuoka, Japan
EMBO J 23:2116-25. 2004..Accumulation of c-Myc was also apparent in mouse Fbw7-/- embryonic stem cells. These observations suggest that two F-box proteins, Fbw7 and Skp2, differentially regulate c-Myc stability by targeting MB1 and MB2, respectively...
- The ubiquitin ligase SCFFbw7 antagonizes apoptotic JNK signalingAbdolrahman S Nateri
Mammalian Genetics Laboratory, Cancer Research UK, London Research Institute, Lincoln s Inn Fields Laboratories, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
Science 303:1374-8. 2004..SCF(Fbw7) therefore antagonizes the apoptotic c-Jun-dependent effector arm of JNK signaling, allowing neurons to tolerate potentially neurotoxic JNK activity...
- CDC4 mutations occur in a subset of colorectal cancers but are not predicted to cause loss of function and are not associated with chromosomal instabilityZoe Kemp
Molecular and Population Genetics Laboratory, London Research Institute, Cancer Research UK
Cancer Res 65:11361-6. 2005..CDC4 mutations may be selected primarily to drive progression through the cell cycle although CIN might be an important secondary effect in some cancers...
- Suprafacial orientation of the SCFCdc4 dimer accommodates multiple geometries for substrate ubiquitinationXiaojing Tang
Centre for Systems Biology, Samuel Lunenfeld Research Institute, Toronto, Ontario, Canada M5G 1X5
Cell 129:1165-76. 2007SCF ubiquitin ligases recruit substrates for degradation via F box protein adaptor subunits. WD40 repeat F box proteins, such as Cdc4 and beta-TrCP, contain a conserved dimerization motif called the D domain...
- Mechanism of auxin perception by the TIR1 ubiquitin ligaseXu Tan
Department of Pharmacology, University of Washington, School of Medicine, Box 357280, Seattle, Washington 98195, USA
Nature 446:640-5. 2007..By filling in a hydrophobic cavity at the protein interface, auxin enhances the TIR1-substrate interactions by acting as a 'molecular glue'. Our results establish the first structural model of a plant hormone receptor...
- Defective cardiovascular development and elevated cyclin E and Notch proteins in mice lacking the Fbw7 F-box proteinMichael T Tetzlaff
Department of Human and Molecular Genetics, Texas Children s Hospital, Houston, TX 77030, USA
Proc Natl Acad Sci U S A 101:3338-45. 2004..These data suggest essential functions for Fbw7 in controlling cyclin E and Notch signaling pathways in the mouse...
- FBXO31 is the chromosome 16q24.3 senescence gene, a candidate breast tumor suppressor, and a component of an SCF complexRaman Kumar
Breast Cancer Genetics Group, Dame Roma Mitchell Cancer Research Laboratories, Department of Medicine, University of Adelaide and Hanson Institute, Institute of Medical and Veterinary Science Adelaide, Australia
Cancer Res 65:11304-13. 2005..We propose that FBXO31 functions as a tumor suppressor by generating SCF(FBXO31) complexes that target particular substrates, critical for the normal execution of the cell cycle, for ubiquitination and subsequent degradation...
- Fbw7 promotes ubiquitin-dependent degradation of c-Myb: involvement of GSK3-mediated phosphorylation of Thr-572 in mouse c-MybK Kitagawa
Department of Biochemistry 1, Hamamatsu University School of Medicine, Higashi ku, Hamamatsu, Shizuoka, Japan
Oncogene 28:2393-405. 2009..In consequence, the c-Myb protein was markedly stabilized by the substitution of Thr-572 to Ala. These observations suggest that SCF(Fbw7) ubiquitin ligase regulates phosphorylation-dependent degradation of c-Myb protein...
- F-box proteins are receptors that recruit phosphorylated substrates to the SCF ubiquitin-ligase complexD Skowyra
Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030, USA
Cell 91:209-19. 1997..SCF complexes couple protein kinase signaling pathways to the control of protein abundance...
- Fbw7 and Usp28 regulate myc protein stability in response to DNA damageNikita Popov
Institute of Molecular Biology and Tumor Research, Emil Mannkopff Str 2, Marburg, Germany
Cell Cycle 6:2327-31. 2007..Our data extend previous observations that link Myc function to the cellular response to DNA damage...
- Mammalian Emi2 mediates cytostatic arrest and transduces the signal for meiotic exit via Cdc20Shisako Shoji
Laboratory of Mammalian Molecular Embryology, RIKEN Center for Developmental Biology, Chuo Ku, Kobe, Japan
EMBO J 25:834-45. 2006..These studies show in intact oocytes that the interaction of Emi2 with Cdc20 links activating stimuli to meiotic resumption at fertilization and during parthenogenesis in mammals...
- Control of hematopoietic stem cell quiescence by the E3 ubiquitin ligase Fbw7Benjamin J Thompson
Department of Pathology, New York University School of Medicine, New York, NY 10016, USA
J Exp Med 205:1395-408. 2008....
- The small-molecule inhibitor BI 2536 reveals novel insights into mitotic roles of polo-like kinase 1Peter Lenart
Research Institute of Molecular Pathology, A 1030 Vienna, Austria
Curr Biol 17:304-15. 2007..Cdk1 and Aurora kinase studies have been facilitated by small-molecule inhibitors, but few if any potent Plk1 inhibitors have been identified...
- Identification of the pollen determinant of S-RNase-mediated self-incompatibilityPaja Sijacic
Integrative Biosciences Graduate Degree Program, The Pennsylvania State University, 403 Althouse Laboratory, University Park, Pennsylvania 16802, USA
Nature 429:302-5. 2004..We conclude that PiSLF encodes the pollen self-incompatibility determinant...
- Mouse Emi2 is required to enter meiosis II by reestablishing cyclin B1 during interkinesisSuzanne Madgwick
Institute for Cell and Molecular Biosciences, The Medical School, University of Newcastle, Newcastle NE2 4HH, England, UK
J Cell Biol 174:791-801. 2006....
- Ubiquitin-dependent degradation of multiple F-box proteins by an autocatalytic mechanismJ M Galan
Swiss Institute for Experimental Cancer Research, Chemin des Boveresses 155, 1066 Epalinges VD, Switzerland
Proc Natl Acad Sci U S A 96:9124-9. 1999....
- Fbxw7 acts as a critical fail-safe against premature loss of hematopoietic stem cells and development of T-ALLSahoko Matsuoka
Department of Cell Differentiation, The Sakaguchi Laboratory, School of Medicine, Keio University, Tokyo 160 8582, Japan
Genes Dev 22:986-91. 2008..Thus, Fbxw7 functions as a fail-safe mechanism against both premature HSC loss and leukemogenesis...
- A role for the anaphase-promoting complex inhibitor Emi2/XErp1, a homolog of early mitotic inhibitor 1, in cytostatic factor arrest of Xenopus eggsJeffrey J Tung
Department of Pathology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, CA 94305, USA
Proc Natl Acad Sci U S A 102:4318-23. 2005..These results identify Emi2 as a candidate CSF maintenance protein...
- Ubiquitylation of cyclin E requires the sequential function of SCF complexes containing distinct hCdc4 isoformsFrank van Drogen
Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
Mol Cell 23:37-48. 2006..Furthermore, in the context of Cdc4alpha and cyclin E, mutational data suggest that Pin1 isomerizes a noncanonical proline-proline bond, with the possibility that Cdc4alpha may serve as a cofactor for altering the specificity of Pin1...
- The SCF ubiquitin ligase: insights into a molecular machineTimothy Cardozo
Department of Pathology and New York University Cancer Institute, New York University Medical Center, 550 First Avenue, MSB 599, New York, New York 10016, USA
Nat Rev Mol Cell Biol 5:739-51. 2004..Here we explore a unifying and structurally detailed view of SCF-mediated proteolytic control of cellular processes that has been revealed by recent studies...
- hCDC4 gene mutations in endometrial cancerCharles H Spruck
Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
Cancer Res 62:4535-9. 2002..Our results show that the hCDC4 gene is mutated in primary human tumors and suggest that it may function as a tumor suppressor in the genesis of many human cancers...
- The tumor suppressor gene hCDC4 is frequently mutated in human T-cell acute lymphoblastic leukemia with functional consequences for Notch signalingAlena Malyukova
Department of Oncology Pathology, Cancer Center Karolinska, Karolinska sjukhuset, Sweden
Cancer Res 67:5611-6. 2007....
- A complex of Cdc4p, Skp1p, and Cdc53p/cullin catalyzes ubiquitination of the phosphorylated CDK inhibitor Sic1pR M Feldman
Division of Biology, California Institute of Technology, Pasadena 91125, USA
Cell 91:221-30. 1997..Taken together, these data illuminate the molecular basis for the G1/S transition in budding yeast and suggest a general mechanism for phosphorylation-targeted ubiquitination in eukaryotes...
- Multisite phosphorylation of a CDK inhibitor sets a threshold for the onset of DNA replicationP Nash
Programme in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto M5G 1X5, Canada
Nature 414:514-21. 2001..Multisite phosphorylation may be a more general mechanism to set thresholds in regulated protein-protein interactions...
- SCF ubiquitin protein ligases and phosphorylation-dependent proteolysisA R Willems
Programme in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada
Philos Trans R Soc Lond B Biol Sci 354:1533-50. 1999..As many F-box proteins can be found in sequence databases, it appears that a host of cellular pathways will be regulated by SCF-dependent proteolysis...
- Fbxw7/Cdc4 is a p53-dependent, haploinsufficient tumour suppressor geneJian Hua Mao
Cancer Research Institute, University of California at San Francisco, 2340 Sutter Street, San Francisco, California 94143, USA
Nature 432:775-9. 2004..We propose that p53-dependent loss of Fbxw7 leads to genetic instability by mechanisms that might involve the activation of Aurora-A, providing a rationale for the early occurrence of these mutations in human cancers...
- Regulation of NF-kappaB by NSD1/FBXL11-dependent reversible lysine methylation of p65Tao Lu
Department of Molecular Genetics, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA
Proc Natl Acad Sci U S A 107:46-51. 2010..We conclude that reversible lysine methylation of NF-kappaB is an important element in the complex regulation of this key transcription factor...
- A direct link of the Mos-MAPK pathway to Erp1/Emi2 in meiotic arrest of Xenopus laevis eggsDaigo Inoue
Department of Biology, Graduate School of Sciences, Kyushu University, Hakozaki 6 10 1, Fukuoka 812 8581, Japan
Nature 446:1100-4. 2007..These results clarify the direct link of the classical Mos-MAPK pathway to Erp1 in meiotic arrest of vertebrate oocytes...
- The F box protein Dsg1/Mdm30 is a transcriptional coactivator that stimulates Gal4 turnover and cotranscriptional mRNA processingMasafumi Muratani
Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
Cell 120:887-99. 2005..We propose that Gal4 ubiquitylation and destruction are required for initiation-competent transcription complexes to transition to fully mature elongating complexes capable of appropriate mRNA processing...
- Mouse Fbw7/Sel-10/Cdc4 is required for notch degradation during vascular developmentRyosuke Tsunematsu
Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan
J Biol Chem 279:9417-23. 2004..Expression of Notch1, -2, or -3 or of cyclin E was unaffected in Fbw7(-/-) embryos. Mammalian Fbw7 thus appears to play an indispensable role in negative regulation of the Notch4-Hey1 pathway and is required for vascular development...
- Mechanisms of tumor suppression by the SCF(Fbw7)Alex C Minella
Division of Clinical Research and Human Biology, Fred Hutchinson Cancer Research Center, Seattle, Washinton 98109 1024, USA
Cell Cycle 4:1356-9. 2005..In this review we discuss the activities of the SCF(Fbw7) in the context of its role as a tumor suppressor and highlight recent findings demonstrating that dominant oncogenes disable Fbw7 function...
- Human F-box protein hCdc4 targets cyclin E for proteolysis and is mutated in a breast cancer cell lineH Strohmaier
Department of Molecular Biology, MB 7, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
Nature 413:316-22. 2001..The gene encoding hCdc4 was found to be mutated in a cell line derived from breast cancer that expressed extremely high levels of cyclin E...
- The ubiquitin-specific protease USP28 is required for MYC stabilityNikita Popov
Institute of Molecular Biology and Tumor Research, Emil Mannkopff Str 2, 35033 Marburg, Germany
Nat Cell Biol 9:765-74. 2007..High expression levels of USP28 are found in colon and breast carcinomas, and stabilization of MYC by USP28 is essential for tumour-cell proliferation...
- The Arabidopsis F-box protein TIR1 is an auxin receptorStefan Kepinski
Department of Biology, University of York, Box 373, York YO10 5YW, UK
Nature 435:446-51. 2005..Here we show that this modification involves the direct binding of auxin to TIR1 and thus that TIR1 is an auxin receptor mediating transcriptional responses to auxin...
- Expression of 10 S-class SLF-like genes in Nicotiana alata pollen and its implications for understanding the pollen factor of the S locusDavid Wheeler
Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Parkville, Victoria 3010, Australia
Genetics 177:2171-80. 2007..Finally, the different topologies of the phylogenetic trees produced using available SLF-related sequences and those produced using S-RNase sequences suggests that pollen S and the S-RNase have different evolutionary histories...
- Plk1 regulates activation of the anaphase promoting complex by phosphorylating and triggering SCFbetaTrCP-dependent destruction of the APC Inhibitor Emi1David V Hansen
Department of Pathology, Stanford University School of Medicine, Palo Alto, CA 94305, USA
Mol Biol Cell 15:5623-34. 2004..These data support the hypothesis that Plk1 activates the APC by directing the SCF-dependent destruction of Emi1 in prophase...
- Met30p, a yeast transcriptional inhibitor that responds to S-adenosylmethionine, is an essential protein with WD40 repeatsD Thomas
Centre de Genetique Moleculaire, Centre National de la Recherche Scientifique, Gif sur Yvette, France
Mol Cell Biol 15:6526-34. 1995..By the two-hybrid method, we showed that Met30p interacts with Met4p and identified a region of Met4p involved in this interaction. Further analysis reveals that expression of Met30p is essential for cell viability...
- Ubiquitination of cyclin-dependent kinase inhibitor, Xic1, is mediated by the Xenopus F-box protein xSkp2Horng Ru Lin
Department of Molecular Medicine, Institute of Biotechnology, University of Texas Health Science Center, San Antonio, Texas 78245 3207, USA
Cell Cycle 5:304-14. 2006..These studies provide the first characterization of the SCF in Xenopus and its role in the ubiquitination of CDK inhibitor, Xic1, during DNA replication initiation...
- Regulating the yeast kinetochore by ubiquitin-dependent degradation and Skp1p-mediated phosphorylationK B Kaplan
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
Cell 91:491-500. 1997..We propose that coupled activation and destruction link the assembly of Cbf3p to the duplication of centromeres in S phase...
- Degradation of Hof1 by SCF(Grr1) is important for actomyosin contraction during cytokinesis in yeastMarc Blondel
CNRS, Station Biologique, UMR7150, Amyloids and Cell Division Cycle Laboratory, Place G Teissier, Roscoff, Bretagne, France
EMBO J 24:1440-52. 2005..SCF(Grr1)-mediated degradation of Hof1 may thus represent a novel mechanism to couple exit from mitosis with initiation of cytokinesis...
- The SV40 large T antigen contains a decoy phosphodegron that mediates its interactions with Fbw7/hCdc4Markus Welcker
Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
J Biol Chem 280:7654-8. 2005..Our data suggest that LT competes with cellular proteins for Fbw7 binding in a substrate-like fashion...
- Xenopus polo-like kinase Plx1 regulates XErp1, a novel inhibitor of APC/C activityAndreas Schmidt
Chemical Biology, Independent Research Group, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany
Genes Dev 19:502-13. 2005..Plx1 phosphorylates XErp1 in vitro at a site that targets XErp1 for degradation upon CSF release. Thus, our data lead to a model of APC/C activation in Xenopus egg extract in which Plx1 targets the APC/C inhibitor XErp1 for degradation...
- Mutations in Fbx4 inhibit dimerization of the SCF(Fbx4) ligase and contribute to cyclin D1 overexpression in human cancerOlena Barbash
Abramson Family Cancer Research Institute, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
Cancer Cell 14:68-78. 2008..Collectively, these data reveal that inactivation of the cyclin D1 E3 ligase likely contributes to cyclin D1 overexpression in a significant fraction of human cancer...
- Competitive interaction between two functional S-haplotypes confer self-compatibility on tetraploid Chinese cherry (Prunus pseudocerasus Lindl. CV. Nanjing Chuisi)S X Huang
College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
Plant Cell Rep 27:1075-85. 2008..Furthermore, in terms of recognition specificity, diploid pollen grains carrying PpsS-3'-haplotype are equal to monoploid pollen grains carrying the other functional S-haplotype...
- NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligasesJidong Liu
Department of Biochemistry and Biophysics, Lineberger Comprehensive Cancer Center, Program in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
Mol Cell 10:1511-8. 2002..We suggest that by restricting SKP1-CUL1 interaction, CAND1 regulated the assembly of productive SCF ubiquitin ligases, allowing a common CUL1-ROC core to be utilized by a large number of SKP1-F box-substrate subcomplexes...
- The APC/C inhibitor, Emi1, is essential for prevention of rereplicationYuichi J Machida
Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
Genes Dev 21:184-94. 2007..These data suggest that Emi1 plays a critical role in preserving genome integrity by blocking rereplication, revealing a previously unrecognized function of this inhibitor of APC/C...
- The C. elegans F-box/WD-repeat protein LIN-23 functions to limit cell division during developmentE T Kipreos
Department of Cellular Biology, The University of Georgia, Athens, Georgia 30602, USA
Development 127:5071-82. 2000..In contrast, lin-23 functions cell autonomously to negatively regulate cell cycle progression, thereby allowing cell cycle exit in response to developmental signals...
- The after-hours mutant reveals a role for Fbxl3 in determining mammalian circadian periodSofia I H Godinho
Medical Research Council MRC Mammalian Genetics Unit, Harwell, Oxfordshire OX11 0RD, UK
Science 316:897-900. 2007..The Afh allele significantly affected Per2 expression and delayed the rate of Cry protein degradation in Per2::Luciferase tissue slices. Our in vivo and in vitro studies reveal a central role for Fbxl3 in mammalian circadian timekeeping...
- Prophase destruction of Emi1 by the SCF(betaTrCP/Slimb) ubiquitin ligase activates the anaphase promoting complex to allow progression beyond prometaphaseFlorence Margottin-Goguet
Department of Pathology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, CA 94305, USA
Dev Cell 4:813-26. 2003..We hypothesize that Emi1 destruction relieves a late prophase checkpoint for APC activation...
- JHDM1B/FBXL10 is a nucleolar protein that represses transcription of ribosomal RNA genesDavid Frescas
Department of Pathology, NYU Cancer Institute, New York University School of Medicine, 550 First Avenue, New York, New York 10016, USA
Nature 450:309-13. 2007....
- Emi1 stably binds and inhibits the anaphase-promoting complex/cyclosome as a pseudosubstrate inhibitorJulie J Miller
Program in Molecular Pharmacology, Stanford University School of Medicine, Stanford, California 94305, USA
Genes Dev 20:2410-20. 2006..The combination of a degron/E3 recognition site and an anti-ligase function in Emi1 suggests a general model for how E3 substrates evolve to become pseudosubstrate inhibitors...
- Ras activity regulates cyclin E degradation by the Fbw7 pathwayAlex C Minella
Division of Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
Proc Natl Acad Sci U S A 102:9649-54. 2005..Thus, we conclude that Ras activity regulates Fbw7-mediated cyclin E proteolysis and suggest that impaired cyclin E proteolysis is a mechanism through which Ras mutations promote tumorigenesis...
- Circadian mutant Overtime reveals F-box protein FBXL3 regulation of cryptochrome and period gene expressionSandra M Siepka
Howard Hughes Medical Institute, Northwestern University, 2205 Tech Drive, Evanston, IL 60208, USA
Cell 129:1011-23. 2007..Thus, Fbxl3(Ovtm) defines a molecular link between CRY turnover and CLOCK/BMAL1-dependent circadian transcription to modulate circadian period...
- Slug stability is dynamically regulated during neural crest development by the F-box protein PpaAnn E Vernon
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208, USA
Development 133:3359-70. 2006..These results provide novel insight into the regulation of Slug, a protein that plays a central role in neural crest precursor formation, as well as in developmental and pathological epithelial to mesenchymal transitions...
- The amino-terminal TPR domain of Dia2 tethers SCF(Dia2) to the replisome progression complexHiroko Morohashi
Cancer Research UK Paterson Institute for Cancer Research, University of Manchester, UK
Curr Biol 19:1943-9. 2009..Our findings suggest that the amino-terminal domains of other F box proteins might also play an analogous regulatory role, controlling the localization of the cognate SCF complexes.
- Phosphorylation of Erp1 by p90rsk is required for cytostatic factor arrest in Xenopus laevis eggsTomoko Nishiyama
Laboratory of Cell and Developmental Biology, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori ku, Yokohama 226 8501, Japan
Nature 446:1096-9. 2007..Thus, Mos and Erp1 collaboratively establish and maintain metaphase II arrest in Xenopus eggs. The link between Mos and Erp1 provides a molecular explanation for the integral mechanism of CSF arrest in unfertilized vertebrate eggs...
- Role of the Schizosaccharomyces pombe F-Box DNA helicase in processing recombination intermediatesTakashi Morishita
Department of Molecular Microbiology, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
Mol Cell Biol 25:8074-83. 2005..Focus formation of Fbh1 protein was induced by treatment that damages DNA. Thus, the F-box DNA helicase appears to process toxic recombination intermediates, the formation of which is dependent on the function of Rhp51...
- Activation of the S-phase checkpoint inhibits degradation of the F-box protein Dia2Andrew C Kile
Department of Genetics, Cell Biology and Development, 6 160 Jackson Hall, 321 Church St, S E, Minneapolis, MN 55455, USA
Mol Cell Biol 30:160-71. 2010..Together, our results suggest that Dia2 protein turnover does not involve an autocatalytic mechanism and that Dia2 proteolysis is inhibited by activation of the replication stress response...
- F-box proteins everywhereEsther Lechner
Institut de Biologie Moleculaire des Plantes du CNRS, 12 rue du Général Zimmer, 67084 Strasbourg Cedex, France
Curr Opin Plant Biol 9:631-8. 2006..Interestingly, viruses and even pathogenic bacteria have also found ways to hijack the plant SCF and to reprogram it for their own purposes...
- Conditional inactivation of Fbxw7 impairs cell-cycle exit during T cell differentiation and results in lymphomatogenesisIchiro Onoyama
Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Higashi ku, Fukuoka 812 8582, Japan
J Exp Med 204:2875-88. 2007....
- The F-Box DNA helicase Fbh1 prevents Rhp51-dependent recombination without mediator proteinsFekret Osman
Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom
Mol Cell Biol 25:8084-96. 2005..Furthermore, analysis of different fbh1 mutant alleles suggests that both the F-box and helicase activities of Fbh1 contribute to controlling Rhp51...
- Inducible dissociation of SCF(Met30) ubiquitin ligase mediates a rapid transcriptional response to cadmiumRegine Barbey
Centre de Genetique Moleculaire, Centre National de la Recherche Scientifique, Gif sur Yvettte, France
EMBO J 24:521-32. 2005..Post-translational control of SCF(Met30) assembly by a physiological stress to allow rapid induction of a protective gene expression program represents a novel mode of regulation in the ubiquitin system...
- SCFFbxl3 controls the oscillation of the circadian clock by directing the degradation of cryptochrome proteinsLuca Busino
Department of Pathology, NYU Cancer Institute, New York University School of Medicine, 550 First Avenue, MSB 599, New York, NY 10016, USA
Science 316:900-4. 2007..Silencing of Fbxl3 produced no effect in Cry1-/-;Cry2-/- cells, which shows that Fbxl3 controls clock oscillations by mediating the degradation of CRY proteins...
- An SCF-like ubiquitin ligase complex that controls presynaptic differentiationEdward H Liao
Department of Medical Genetics and Microbiology, Samuel Lunenfeld Research Institute, University of Toronto, Ontario, Canada M5G 1X5
Nature 430:345-50. 2004..This neuron-specific, SCF-like complex therefore provides a localized signal to attenuate presynaptic differentiation...
- SIC1 is ubiquitinated in vitro by a pathway that requires CDC4, CDC34, and cyclin/CDK activitiesR Verma
Division of Biology, California Institute of Technology, Pasadena 91125, USA
Mol Biol Cell 8:1427-37. 1997..The complementary C-terminal segment of SIC1 binds to the S-phase cyclin CLB5, indicating a modular structure for SIC1...
- Fus3-regulated Tec1 degradation through SCFCdc4 determines MAPK signaling specificity during mating in yeastSong Chou
Department of Biological Chemistry, University of California, Irvine, Irvine, CA 92697, USA
Cell 119:981-90. 2004..Tec1T273V blocks Tec1 ubiquitination and degradation and allows the induction of filamentation genes in response to pheromone. Thus, Fus3 inhibits filamentous growth during mating by degrading Tec1...
- Regulation of the cell cycle by SCF-type ubiquitin ligasesKeiichi I Nakayama
Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka, Fukuoka 812 8582, Japan
Semin Cell Dev Biol 16:323-33. 2005..Clinical evidence also suggests that dysregulation of these F-box proteins contributes to human cancers...
- The F-box protein TIR1 is an auxin receptorNihal Dharmasiri
Department of Biology, Indiana University, Bloomington, Indiana 47405, USA
Nature 435:441-5. 2005..Finally, TIR1 synthesized in insect cells binds Aux/IAA proteins in an auxin-dependent manner. Together, these results indicate that TIR1 is an auxin receptor that mediates Aux/IAA degradation and auxin-regulated transcription...
- The evi5 oncogene regulates cyclin accumulation by stabilizing the anaphase-promoting complex inhibitor emi1Adam G Eldridge
Department of Cancer Biology, Stanford University School of Medicine, CA 94305, USA
Cell 124:367-80. 2006..We propose that the balance of Evi5 and Polo-like kinase activities determines the timely accumulation of Emi1 and cyclin, ensuring mitotic fidelity...
- Calcium elevation at fertilization coordinates phosphorylation of XErp1/Emi2 by Plx1 and CaMK II to release metaphase arrest by cytostatic factorJunjun Liu
Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine, Denver, Colorado 80262, USA
Curr Biol 15:1458-68. 2005..However, connections between the Plx1 pathway and the CaMKII pathway have not been identified...
- Fission yeast Mcl1 interacts with SCF(Pof3) and is required for centromere formationYasmine M Mamnun
Laboratory of Cell Regulation, London Research Institute, Cancer Research UK, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
Biochem Biophys Res Commun 350:125-30. 2006..Importantly, we identity a novel, specific role for Mcl1 in the transcriptional silencing and the localisation of CENP-A at the centromeres...
- sel-10, a negative regulator of lin-12 activity in Caenorhabditis elegans, encodes a member of the CDC4 family of proteinsE J Hubbard
Department of Biochemistry and Molecular Biophysics, Howard Hughes Medical Institute, New York, New York 10032, USA
Genes Dev 11:3182-93. 1997..We propose that SEL-10 promotes the ubiquitin-mediated turnover of LIN-12/Notch proteins, and discuss potential roles for the regulation of lin-12/Notch activity by sel-10 in cell fate decisions and tumorigenesis...
- CaMKII and polo-like kinase 1 sequentially phosphorylate the cytostatic factor Emi2/XErp1 to trigger its destruction and meiotic exitDavid V Hansen
Program in Cancer Biology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, CA 94305, USA
Proc Natl Acad Sci U S A 103:608-13. 2006....
- Overexpression of the anaphase promoting complex/cyclosome inhibitor Emi1 leads to tetraploidy and genomic instability of p53-deficient cellsNorman L Lehman
Department of Pathology, Stanford University, Stanford, California 94305, USA
Cell Cycle 5:1569-73. 2006..This represents a potentially important mechanism by which pRb and p53 dysfunction may contribute to tumorigenesis through the generation of genomic instability...
- The v-Jun point mutation allows c-Jun to escape GSK3-dependent recognition and destruction by the Fbw7 ubiquitin ligaseWenyi Wei
Department of Medical Oncology, Dana Farber Cancer Institute and Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
Cancer Cell 8:25-33. 2005..These findings explain the enhanced stability and oncogenicity of v-Jun relative to its cellular counterpart and reveal that GSK3 and Fbw7 coordinately regulate c-Jun and c-Myc...
- Role of Polo-like kinase in the degradation of early mitotic inhibitor 1, a regulator of the anaphase promoting complex/cyclosomeYakir Moshe
Unit of Biochemistry, The Bruce Rappaport Faculty of Medicine, Technion Israel Institute of Technology, Haifa 31096, Israel
Proc Natl Acad Sci U S A 101:7937-42. 2004..Transfection with an small interfering RNA duplex directed against Plk1 caused the accumulation of Emi1 in mitotically arrested HeLa cells. It is suggested that phosphorylation of Emi1 by Plk1 is involved in its degradation in mitosis...
- Multisite phosphorylation and the countdown to S phaseR J Deshaies
Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
Cell 107:819-22. 2001..This numerical wizardry suggests how analog inputs can be rectified to digital outputs. Unraveling the counting mechanism promises to generate new insights into the architecture of protein nanoprocessors...
- Regulation of the G1 to S transition by the ubiquitin pathwayL M DeSalle
Department of Pathology and Kaplan Comprehensive Cancer Center, MSB 548, New York University Medical Center, 550 First Avenue, New York, NY 10016, USA
FEBS Lett 490:179-89. 2001..Thus, understanding the mechanisms that confer specificity to the ubiquitin system will allow the development of novel therapeutic approaches to target aberrations in this pathway underlying tumorigenesis and other human pathologies...
- Archipelago regulates Cyclin E levels in Drosophila and is mutated in human cancer cell linesK H Moberg
Massachusetts General Hospital Cancer Center, Charlestown, MA 02129, USA
Nature 413:311-6. 2001..These findings implicate archipelago in developmentally regulated degradation of Cyclin E and potentially in the pathogenesis of human cancers...
- E2F-dependent accumulation of hEmi1 regulates S phase entry by inhibiting APC(Cdh1)Jerry Y Hsu
Department of Pathology and Program in Biophysics, Stanford University School of Medicine, CA 94305, USA
Nat Cell Biol 4:358-66. 2002..These data suggest that E2F can activate both transcription of cyclin A and the hEmi1-dependent stabilization of APC(Cdh1) targets, such as cyclin A, to promote S phase entry...
- Skp1p and the F-box protein Rcy1p form a non-SCF complex involved in recycling of the SNARE Snc1p in yeastJ M Galan
Swiss Institute for Experimental Cancer Research, 1066 Epalinges VD, Switzerland
Mol Cell Biol 21:3105-17. 2001..Importantly, Skp1p was the only major partner that copurified with Rcy1p. Our results thus suggest that a complex composed of Rcy1p and Skp1p but not other SCF components may play a direct role in recycling of internalized proteins...
- An essential domain within Cdc34p is required for binding to a complex containing Cdc4p and Cdc53p in Saccharomyces cerevisiaeN Mathias
Department of Biochemistry and Molecular Biology, Indiana University School of Medicine and the Walther Oncology Center, Indianapolis, Indiana, 46202 5122, USA
J Biol Chem 273:4040-5. 1998..This suggests that all Cdc34p substrates are ubiquitinated by a similar high molecular weight complex...
- Fbxw7 contributes to tumor suppression by targeting multiple proteins for ubiquitin-dependent degradationYo Fujii
Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582
Cancer Sci 97:729-36. 2006..Fbxw7 seems to regulate the levels of multiple targets to suppress cancer development...
- Grr1 functions in the ubiquitin pathway in Saccharomyces cerevisiae through association with Skp1T Kishi
Department of Molecular Genetics, National Institute of Genetics, Shizuoka ken, Japan
Mol Gen Genet 257:143-8. 1998..Furthermore, Grr1 bound Skp1 directly in vitro. These results strongly suggest that Grr1 functions in the ubiquitin pathway through association with Skp1...
- A NOVEL F BOX PROTEIN REGULATING MITOSISPeter Jackson; Fiscal Year: 2004..Understanding how SBP5 blocks mitosis and induces apoptosis may provide important insight for creating new cancer chemotherapeutic drugs that act similarly or synergistically with vinca alkaloids and Taxol. ..
- Mechanistic validation of SCF E3 ligase as a cancer and radiosensitizing targetYi Sun; Fiscal Year: 2013..This study will validate SCF E3 ubiquitin ligase as an anticancer and radiosensitizing target and MLN4924, a newly discovered SCF E3 ligase inhibitor, as a tumor-selective and radiosensitizing agent for cancer therapy. ..
- Orientia tsutsugamushi modulation of host cell ubiquitination machineryJason A Carlyon; Fiscal Year: 2013..In Aim 2, we will close an important knowledge gap by identifying the Anks that the pathogen expresses as it establishes infection in each of the 3 biologically relevant host cells. ..
- Role of SAG/RBX2 E3 Ubiquitin Ligase in Skin CarcinogenesisYi Sun; Fiscal Year: 2013....
- Mechanisms of tumor suppression by Fbw7Jonathan E Grim; Fiscal Year: 2010..As such, it is a crucial step towards developing treatment strategies for tumors associated with this pathway. ..
- SAG E3 ubiquitin ligase in regulation of transformation and carcinogenesisYi Sun; Fiscal Year: 2013....
- Role of the SCF/FBX011 ubiquitin ligase in the control of cell proliferationMichele Pagano; Fiscal Year: 2013..Rossi. ..
- Role of the SCF/Fbxo15 ubiquitin ligase in stem cells and cancer biologyMichele Pagano; Fiscal Year: 2012..We will identify biologically significant substrates of human Fbxo15 (Specific Aim 1) and validate the Fbxo15-dependent regulation of these substrates (Specific Aim 2). ..
- Structural studies of substrate recognition and specificity by the SCF ubiquitinBing Hao; Fiscal Year: 2013....
- Anti-pancreatic tumorigenesis by inactivation of SAG/RBX2 E3 ubiquitin ligaseYi Sun; Fiscal Year: 2013..Our work is, therefore, highly innovative and of significant impact with translational value. ..
- Regulation of Innate Immunity by F-Box ProteinsBeibei Chen; Fiscal Year: 2013....
- Septic Lung Injury and SurfactantRama K Mallampalli; Fiscal Year: 2013..Execution of studies outlined in this application will provide a major advance in the conceptual framework for how sepsis causes lung impairment by altering surfactant production. ..
- Evolution of Developmental Regulatory PathwaysRonald E Ellis; Fiscal Year: 2012..This project will elucidate how the functions of genes change during evolution, which could help scientists choose which creatures to study, and avoid mistaken inferences about their medical significance. ..
- Determinants of Protein Degradation Dynamics in Cell SignalingBRITNEY LANE MOSS; Fiscal Year: 2013..Thus, this research will both improve basic understanding of mechanisms that drive cancer and aid in designing therapeutics that specifically target dysfunctional signaling pathways in cancer cells. ..
- Regulation of Cellular Growth and Division by Ubiquitin-Mediated ProteolysisJennifer A Benanti; Fiscal Year: 2012..We will analyze SCF complex composition in different cell cycle and growth states to address these questions. ..
- Global characterization of ubiquitin information networks with mass spectrometrySteven P Gygi; Fiscal Year: 2013..Upon completion, our next step will be to create the complementary information networks for human genes. ..
- Mechanism of Auxin ActionMARK A ESTELLE; Fiscal Year: 2012..Defects in the pathway contribute to many disease processes including cancers. This study will advance our understanding of the ubiquitin/proteasome pathway in cell function. ..
- The Role of FBXL4 in T-cell Acute Lymphoblastic LeukemiaJULIE ERIKA M HAYDU; Fiscal Year: 2013..Overall, these studies will characterize the roles and mechanisms of FBXL4 in the pathogenesis of T-ALL. ..
- Regulation of cullin-RING ligases by Nedd8Raymond J Deshaies; Fiscal Year: 2013....
- F-box proteins: deregulated cell cycle control and proteolysis in cancerMichele Pagano; Fiscal Year: 2013..As the mechanisms of the ubiquitin-mediated proteolysis of cell cycle regulators are unraveled, this team is committed to the integration of its basic research results with an understanding of malignant transformation. ..
- Regulation of Cellular Growth and Division by Ubiquitin-Mediated ProteolysisJENNIFER BENANTI; Fiscal Year: 2009..The cell division cycle is regulated by the timed synthesis and destruction of regulatory molecules. Understanding how timed destruction of these molecules occurs will increase our understanding of processes disrupted in cancer cells. ..
- MECHANISMS OF CELL CYCLE CONTROL DURING MAMMARY GLAND DEHui Zhang; Fiscal Year: 2001..These studies should also provide a basis to design novel strategies for diagnosis, prognosis, and therapeutic treatment of breast cancers. ..
- Phosphoserine Dependant Assembly of Signaling complexesMichael Yaffe; Fiscal Year: 2007..unreadable] [unreadable]..
- Novel Assays to Identify HIV Vif InhibitorsDana Gabuzda; Fiscal Year: 2006..unreadable] [unreadable] [unreadable] [unreadable]..
- Analysis of ASKs, Components of SCF Ubiquitin-LigasesDEREK GINGERICH; Fiscal Year: 2005..abstract_text> ..
- F box Proteins and Deregulated Proteolysis in CancerMichele Pagano; Fiscal Year: 2005..This will be achieved using specific membrane permeable peptides designed on the basis of our structural and biochemical information. ..
- Functional Dissection of an F-box Protein in DevelopmentMichael Weir; Fiscal Year: 2006..abstract_text> ..
- Analysis of Fbx2 Family of Ubiquitin LigasesHenry Paulson; Fiscal Year: 2007..In three complementary aims, we will determine whether F box proteins encoded by the Fbx2 gene family function as ubiquitin ligases to mediate glycoprotein quality control in ..
- Cell cycle and transcriptional control by F box proteinsCurt Wittenberg; Fiscal Year: 2005..Understanding these regulatory mechanisms is central to understanding a range of human conditions including cancer, developmental defects and heritable disease. ..
- SAG E3 ubiquitin ligase in angiogenesis & carcinogenesisYi Sun; Fiscal Year: 2010..Targeting SAG in cancer cells, via siRNA silencing or small molecule inhibitors, would, therefore, be a valid antianglogenesis therapy. ..
- Establishment of C.elegans tissue culture cell linesEDWARD KIPREOS; Fiscal Year: 2007..The availability of cell lines would greatly extend research using this organism to provide further health-related insights. [unreadable] [unreadable]..
- Protein Knockout Technology/Molecular Analysis of CancerPengbo Zhou; Fiscal Year: 2004....
- Ubiquitin-Proteolytic Control of HOXA9 in LeukemogenesisPengbo Zhou; Fiscal Year: 2010..abstract_text> ..
- Substrates of SAG-SCF E3 ligase regulate hypoxia responseYi Sun; Fiscal Year: 2007..These initial studies will set the stage for a more detailed investigation of the mechanism of SAG mediated suppression of apoptosis. [unreadable] [unreadable]..
- The role of Cyclin E in growth control and tumorogenesisSteven Reed; Fiscal Year: 2008..It is hoped that insights gained from understanding these fundamental mechanisms of oncogenesis will lead to new approaches to cancer prevention and therapy. ..
- Regulation of Nucleotide Excision Repair by ProteolysisPengbo Zhou; Fiscal Year: 2008..abstract_text> ..
- Genetics of Cell Cycle Regulators in C. elegansEdward T Kipreos; Fiscal Year: 2010..The abstract has been modified to eliminate the previously proposed screen to identify cullin PTMs. ..
- THE S. CEREVISIAE S PHASE CHECKPOINTSteven Reed; Fiscal Year: 2003....
- Receptor-Mediated Endocytosis of Legionella PneumophilaYousef Abu Kwaik; Fiscal Year: 2004..Our studies may uncover potential pathogenic evolution of Lpn to invade the more evolved mammalian cells, and may contribute to the understanding of invasion of protozoa by Mycobacterium and Chlamydia. ..
- Stress proteins in autoimmune depigmentationISABELLE LE POOLE; Fiscal Year: 2005..The same model will be of use to test the efficacy of therapeutic modalities to be developed for vitiligo in the future. ..
- Pigmentation and Diversity ConferenceISABELLE LE POOLE; Fiscal Year: 2007..unreadable] [unreadable] [unreadable] [unreadable]..
- The Pore-forming Toxin of Legionella PneumophilaYousef Abu Kwaik; Fiscal Year: 2003..Our studies on L. pneumophila may provide a paradigm for other vacuolar intracellular pathogens such as Mycobacterium, Salmonella, and Chlamydia. ..
- Hedgehog signaling as a novel regulator of hematopoiesisIannis Aifantis; Fiscal Year: 2007..We will complement these experiments with the study of novel Hh activating mouse mutants in which we will define the exact role of the Gli factors in the regulation of mammalian lymphopoiesis and hematopoiesis. ..
- Regulation and Function of MAPKAP KinasesThomas Sturgill; Fiscal Year: 2005..Aim four is to characterize the activation, enzymatic properties, and functions of MAPKAP kinase-like enzymes (Rcklp, Rck2p) in yeast, a model eukaryote, using combined biochemical and genetic approaches. ..
- BIACORE 3000 BiosensorCecile Pickart; Fiscal Year: 2004..In recognition of this fact, the Bloomberg School of Public Health has made a substantial commitment to the long-term operation and maintenance of the Biacore 3000 ..
- GENETIC ANALYSIS OF EARLY LIMB DEVELOPMENTArend Sidow; Fiscal Year: 2004..Because Dac is likely to be the mouse ortholog of the human Split Hand/Foot Malformation 3 gene, this project may also bear direct relevance to understanding and diagnosis of congenital limb diseases. ..
- ASCB Conf:Nontraditional Functions of Ubiquitin and UbLsCecile Pickart; Fiscal Year: 2002..There will also be a poster session. The format of the Conference, and its small size, are designed to foster interactions among the 200 participants. ..
- Genistein induces thymic atrophy: a health concern?Paul Cooke; Fiscal Year: 2006..These studies will advance our understanding of isoflavone effects on the immune system, and also may have relevance for soy-fed infants and laboratory rodents consuming soy-based feed. ..
- The ProPhylER Database and Web ResourceArend Sidow; Fiscal Year: 2006..unreadable] [unreadable]..
- Autoimmune vitiligo as a roadmap to melanoma therapyISABELLE LE POOLE; Fiscal Year: 2007..Importantly, the cloning of high affinity T cell receptors in retroviral vectors may be suitable for introduction into patient PBL for treatment of malignant melanoma. ..
- The Ciona savignyi Genetic MapArend Sidow; Fiscal Year: 2008..Insights into the molecular mechanisms of regulation and development using Ciona as a model are highly effective and relevant to the homologous processes in human. ..
- Chemopreventive treatment of familial melanomaISABELLE LE POOLE; Fiscal Year: 2008..3] To quantify melanoma tumor growth and measure immunologic parameters (cytokine secretion by ELISA and ELISPOT) in mice treated with bleaching agents and IL-2. [unreadable] [unreadable] [unreadable]..
- Regulation of interferon receptor by Leishmania kinaseSERGE FUCHS; Fiscal Year: 2008..unreadable] [unreadable] [unreadable]..
- Role of the Archipelago gene in Drosophila tracheal morphogenesisKenneth H Moberg; Fiscal Year: 2010..Thus, we hypothesize that ago plays an important role in the homeostatic mechanisms that restrain tracheal branching. Our goal in Specific Aim 3 is to define this role and elucidate its molecular basis. ..
- Molecular pathogenesis of Francisella tularnesisYOUSEF A ABU KWAIK; Fiscal Year: 2010..The bacterial effectors involved in exploiting the macrophage are potential targets for treatment as well as potential vaccine candidates. ..
- Interaction of auxin and abscisic acid signalingLUCIA STRADER; Fiscal Year: 2007..By studying and cloning suppresors, as well as identifying IBR5 substrates, a more in-depth understanding of auxin and ABA cross-talk will be gained. ..
- Stability of prolactin receptor and prolactin signalingSerge Y Fuchs; Fiscal Year: 2010....
- CELL CYCLE ARREST AND SIGNAL TRANSDUCTION IN YEASTELAINE ELION; Fiscal Year: 2009..abstract_text> ..
- mTOR Signaling PathwaysThomas W Sturgill; Fiscal Year: 2010..We will investigate novel candidate mTOR-interacting proteins identified by MS. Finally, to understand better the mechanisms involved in mTORC2, we propose to identify pianissimo-interacting proteins. ..
- Skyscan 1172 ex-vivo microComputed Tomography SystemFrancesco Ramirez; Fiscal Year: 2007..Acquisition of this equipment will enable our institutions to engage in biomedical research highly relevant to pathophysiological processes and animal models of human diseases. [unreadable] [unreadable] [unreadable]..
- A transgenic model of ocular inflammationRussell Van Gelder; Fiscal Year: 2007..unreadable] [unreadable]..
- DEVELOPMENTAL BIOLOGY AND GENETICS OF THE MAIZE LEAFMichael Freeling; Fiscal Year: 2001..We are elucidating a molecular genetic basis for heterochrony, the prevailing theory that links evolution with developmental biology. ..