hemoglobin a

Summary

Summary: Normal adult human hemoglobin. The globin moiety consists of two alpha and two beta chains.

Top Publications

  1. ncbi The clinical sequelae of intravascular hemolysis and extracellular plasma hemoglobin: a novel mechanism of human disease
    Russell P Rother
    Alexion Pharmaceuticals, Cheshire, Conn 06410, USA
    JAMA 293:1653-62. 2005
  2. ncbi A comparison of anemia in hemoglobin C and normal hemoglobin A children with Plasmodium falciparum malaria
    Dapa A Diallo
    faculté de médecine de pharmacie et d odontostomatologie, University of Bamako, Bamako, Mali
    Acta Trop 90:295-9. 2004
  3. pmc Mechanism of human Hb switching: a possible role of the kit receptor/miR 221-222 complex
    Marco Gabbianelli
    Department of Hematology, Oncology and Molecular Medicine, Istituto Superiore di Sanita, 00161 Rome, Italy
    Haematologica 95:1253-60. 2010
  4. pmc Aggregation of normal and sickle hemoglobin in high concentration phosphate buffer
    Kejing Chen
    Department of Physics, Wake Forest University, Winston Salem, North Carolina, USA
    Biophys J 87:4113-21. 2004
  5. pmc Fetal hemoglobin silencing in humans
    Patricia A Oneal
    Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 10 Center Drive, Bldg 10, Rm 9B17, Bethesda, MD 20892, USA
    Blood 108:2081-6. 2006
  6. ncbi Sites of nonenzymatic glycosylation of human hemoglobin A
    R Shapiro
    J Biol Chem 255:3120-7. 1980
  7. ncbi Human reticulocytes isolated from peripheral blood: maturation time and hemoglobin synthesis
    Oyvind Skadberg
    Institute of Clinical Biochemistry, Haukeland University Hospital, University of Bergen, Bergen, Norway
    Lab Hematol 9:198-206. 2003
  8. doi Comparison of Sebia Capillarys capillary electrophoresis with the Primus high-pressure liquid chromatography in the evaluation of hemoglobinopathies
    David F Keren
    Warde Medical Laboratory, St Joseph Mercy Hospital, Ann Arbor, MI 48108, USA
    Am J Clin Pathol 130:824-31. 2008
  9. ncbi Evaluation of a new Sebia kit for analysis of hemoglobin fractions and variants on the Capillarys system
    Amal Louahabi
    Department of Clinical Chemistry, University Hospital St Luc, Brussels, Belgium
    Clin Chem Lab Med 44:340-5. 2006
  10. ncbi A third quaternary structure of human hemoglobin A at 1.7-A resolution
    M M Silva
    Department of Biochemistry, University of Iowa, Iowa City 52242
    J Biol Chem 267:17248-56. 1992

Research Grants

  1. OXYGEN TRANSPORT CYS CELL FREE HEMOGLOBIN
    Robert Winslow; Fiscal Year: 1999
  2. OXYGEN TRANSPORT CYS CELL FREE HEMOGLOBIN
    Robert Winslow; Fiscal Year: 2000
  3. OXYGEN TRANSPORT CYS CELL FREE HEMOGLOBIN
    Robert Winslow; Fiscal Year: 2001
  4. OXYGEN TRANSPORT CYS CELL FREE HEMOGLOBIN
    Robert Winslow; Fiscal Year: 2002
  5. Heme Oxygenase and Spinal Cord Injury
    Linda Noble; Fiscal Year: 2004
  6. Heme Oxygenase and Spinal Cord Injury
    Linda Noble; Fiscal Year: 2002
  7. Heme Oxygenase and Spinal Cord Injury
    Linda Noble; Fiscal Year: 2001
  8. Heme Oxygenase and Spinal Cord Injury
    Linda Noble; Fiscal Year: 2003
  9. METALLOPROTEIN NITROSYL FORMATION AND REACTIVITY
    Michael Doyle; Fiscal Year: 1980
  10. Role of nitrite and hemoglobin in vascular NO homeostasis in the fetus and adult
    Arlin B Blood; Fiscal Year: 2010

Detail Information

Publications199 found, 100 shown here

  1. ncbi The clinical sequelae of intravascular hemolysis and extracellular plasma hemoglobin: a novel mechanism of human disease
    Russell P Rother
    Alexion Pharmaceuticals, Cheshire, Conn 06410, USA
    JAMA 293:1653-62. 2005
    ..A growing list of clinical manifestations attributed to hemoglobin release in a variety of acquired and iatrogenic hemolytic disorders suggests that hemolysis and hemoglobinemia should be considered as a novel mechanism of human disease...
  2. ncbi A comparison of anemia in hemoglobin C and normal hemoglobin A children with Plasmodium falciparum malaria
    Dapa A Diallo
    faculté de médecine de pharmacie et d odontostomatologie, University of Bamako, Bamako, Mali
    Acta Trop 90:295-9. 2004
    ..Senescence in hemoglobin C (HbC) erythrocytes relative to normal hemoglobin A (HbA) erythrocytes is also increased, presumably because of the sensitivity of HbC to oxidative damage and ..
  3. pmc Mechanism of human Hb switching: a possible role of the kit receptor/miR 221-222 complex
    Marco Gabbianelli
    Department of Hematology, Oncology and Molecular Medicine, Istituto Superiore di Sanita, 00161 Rome, Italy
    Haematologica 95:1253-60. 2010
    ..MicroRNAs (miRs) play a pivotal role in normal hematopoiesis: downmodulation of miR-221/222 stimulates human erythropoietic proliferation through upmodulation of the kit receptor...
  4. pmc Aggregation of normal and sickle hemoglobin in high concentration phosphate buffer
    Kejing Chen
    Department of Physics, Wake Forest University, Winston Salem, North Carolina, USA
    Biophys J 87:4113-21. 2004
    ..In this article, we show that hemoglobin S and normal adult hemoglobin, hemoglobin A, aggregate in high concentration phosphate buffer even when the concentration of hemoglobin is below the ..
  5. pmc Fetal hemoglobin silencing in humans
    Patricia A Oneal
    Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 10 Center Drive, Bldg 10, Rm 9B17, Bethesda, MD 20892, USA
    Blood 108:2081-6. 2006
    ..The silencing phenomenon is mediated by a mechanism of cellular replacement. This novel silencing pattern may be important for the development of HbF-enhancing therapies...
  6. ncbi Sites of nonenzymatic glycosylation of human hemoglobin A
    R Shapiro
    J Biol Chem 255:3120-7. 1980
  7. ncbi Human reticulocytes isolated from peripheral blood: maturation time and hemoglobin synthesis
    Oyvind Skadberg
    Institute of Clinical Biochemistry, Haukeland University Hospital, University of Bergen, Bergen, Norway
    Lab Hematol 9:198-206. 2003
    ..Taking into account loss of hemoglobin-containing vesicles during reticulocyte maturation, we estimated that more than 20% of hemoglobin in erythrocytes was synthesized in reticulocytes after release from bone marrow...
  8. doi Comparison of Sebia Capillarys capillary electrophoresis with the Primus high-pressure liquid chromatography in the evaluation of hemoglobinopathies
    David F Keren
    Warde Medical Laboratory, St Joseph Mercy Hospital, Ann Arbor, MI 48108, USA
    Am J Clin Pathol 130:824-31. 2008
    ..Both methods identified HbS, HbC, HbE, HbS, and HbC together, HbA2&prime, HbD-Los Angeles, HbF variant, HbG-Philadelphia, HbS-G Philadelphia, and Hb Lepore...
  9. ncbi Evaluation of a new Sebia kit for analysis of hemoglobin fractions and variants on the Capillarys system
    Amal Louahabi
    Department of Clinical Chemistry, University Hospital St Luc, Brussels, Belgium
    Clin Chem Lab Med 44:340-5. 2006
    ..1-3.2%. The Capillarys system is a fully automated and accurate system that gives high-resolution performance and displays appropriate characteristics for use as a routine method for the diagnosis of thalassemias and hemoglobinopathies...
  10. ncbi A third quaternary structure of human hemoglobin A at 1.7-A resolution
    M M Silva
    Department of Biochemistry, University of Iowa, Iowa City 52242
    J Biol Chem 267:17248-56. 1992
    Previous crystallographic studies have shown that human hemoglobin A can adopt two stable quaternary structures, one for deoxyhemoglobin (the T-state) and one for liganded hemoglobin (the R-state)...
  11. pmc Molecular dynamics simulations of hemoglobin A in different states and bound to DPG: effector-linked perturbation of tertiary conformations and HbA concerted dynamics
    Monique Laberge
    Department of Biochemistry and Biophysics and Johnson Research Foundation, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania, USA
    Biophys J 94:2737-51. 2008
    ..The changes are not apparent when comparing the available x-ray crystal structures in the presence and absence of effector, but are striking when comparing the respective dynamic tertiary conformations of the R and T tetramers...
  12. pmc Restoration of hemoglobin A synthesis in erythroid cells from peripheral blood of thalassemic patients
    G Lacerra
    Lineberger Comprehensive Cancer Center and Department of Pharmacology, University of North Carolina, Chapel Hill, NC 27599 7295, USA
    Proc Natl Acad Sci U S A 97:9591-6. 2000
    ..Thus, expression of defective beta-globin genes was repaired and significant level of Hb A was restored in a cell population that would be targeted in clinical applications of this approach...
  13. ncbi High-level expression of hemoglobin A in human thalassemic erythroid progenitor cells following lentiviral vector delivery of an antisense snRNA
    Marla M Vacek
    Curriculum in Genetics and Molecular Biology, University of North Carolina Gene Therapy Center, Department of Pharmacology and Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, 27599, USA
    Blood 101:104-11. 2003
    ..623 lentiviral vector, the levels of correctly spliced beta-globin mRNA and hemoglobin A were approximately 25-fold over background...
  14. ncbi Binding of ovarian steroids to erythrocytes in patients with sickle cell disease; effects on cell sickling and osmotic fragility
    Wai C Yoong
    University Department of Obstetrics and Gynaecology, North Middlesex Hospital, Sterling Way, London N18 1QX, UK
    J Steroid Biochem Mol Biol 84:71-8. 2003
    ..However, steroid binding does not affect susceptibility to sickling or osmotic fragility irrespective of haemoglobin genotype...
  15. ncbi Fluorescence measurements of the labile iron pool of sickle erythrocytes
    Deepika Darbari
    Center for Sickle Cell Disease, Department of Biophysics and Physiology, University Hospital, Howard University College of Medicine, 2121 Georgia Avenue NW, Washington, DC 20060, USA
    Blood 102:357-64. 2003
    ..We conclude that cytosolic labile iron is not elevated in hemoglobin SS erythrocytes and that elemental membrane iron is present in only trace amounts...
  16. ncbi Regioselective covalent modification of hemoglobin in search of antisickling agents
    Soobong Park
    Department of Medicinal Chemistry and Pharmacognosy and Center for Pharmaceutical Biotechnology, College of Pharmacy, University of Illinois at Chicago, 833 S Wood Street, Chicago, Illinois 60612, USA
    J Med Chem 46:936-53. 2003
    ....
  17. ncbi Effect of nitric oxide on the transport and release of oxygen in fetal blood
    Maria Elisabetta Clementi
    CNR Institute Chimica del riconoscimento Molecolare, Catholic University, Largo F Vito 1, 00168 Rome, Italy
    Biochem Biophys Res Commun 302:515-9. 2003
    ....
  18. ncbi Distinct domain responses of R-state human hemoglobins A, C, and S to anions
    Qiuying Chen
    Department of Medicine, Division of Hematology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Blood Cells Mol Dis 29:119-32. 2002
    ....
  19. ncbi Rapid prenatal diagnosis of sickle cell diseases using oligonucleotide ligation assay coupled with laser-induced capillary fluorescence detection
    Nancy S Day
    Division of Pediatric Hematology, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
    Prenat Diagn 22:686-91. 2002
    ..This new approach allows the diagnosis of both Hgb S and Hgb C to be available in a few hours. We have utilized this technique in 30 prenatal diagnoses and found it to be in complete agreement with the standard diagnoses...
  20. ncbi Oxygen saturation and hemoglobin A content in patients with sickle cell disease undergoing erythrocytapheresis
    Thomas P Nifong
    Department of Pathology, Penn State Milton S Hershey Medical Center, Pennsylvania State University College of Medicine, Hershey, Pennsylvania, USA
    Ther Apher 6:390-3. 2002
    ..We investigated the changes in oxygen saturation versus hematocrit, fraction of hemoglobin A, and transfusion volume during 71 prophylactic erythrocytapheresis procedures performed in 5 stable patients ..
  21. ncbi Effects of fetal hemoglobin on accurate measurements of oxygen saturation in neonates
    Shyang Yun Pamela K Shiao
    School of Nursing, University of Texas Health Science Center at Houston, Houston, TX 77030, USA
    J Perinat Neonatal Nurs 19:348-61. 2005
    ..To examine the accuracy of oxygen saturation (So(2)) in relation to blood oxyhemoglobin (Hbo(2)) measurements with the effects of fetal hemoglobin (HbF) determined and their oxyhemoglobin dissociation curves...
  22. ncbi Analysis of betaS and betaA genes in a Mexican population with African roots
    María Teresa Magaña
    Division de Genetica, Centro de Investigacion Biomedica de Occidente, CMNO, IMSS, Sierra Mojada No 800, Col Independencia, Guadalajara, Mexico
    Blood Cells Mol Dis 28:121-6. 2002
    ..These data suggest distinct origins for the beta(A) and beta(S) genes in Mexican individuals from the Costa Chica region...
  23. ncbi Influence of carriage of hemoglobin AS and the Fc gamma receptor IIa-R131 allele on levels of immunoglobulin G2 antibodies to Plasmodium falciparum merozoite antigens in Gabonese children
    Francine Ntoumi
    Unite de Recherches Medicales, Hopital Albert Schweitzer, Lambarene, Gabon
    J Infect Dis 192:1975-80. 2005
    ..falciparum strains were investigated...
  24. ncbi A biophysical investigation of recombinant hemoglobins with aromatic B10 mutations in the distal heme pockets
    Mary Ellen Wiltrout
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA
    Biochemistry 44:7207-17. 2005
    ..These results may provide new insights for designing a new generation of hemoglobin-based oxygen carriers...
  25. ncbi Anion transport in normal erythrocytes, sickle red cells, and ghosts in relation to hemoglobins and magnesium
    D Teti
    Institute of General Pathology, Faculty of Medicine and Surgery, Torre Biologica IV Piano, Policlinico G Martino, Via Consolare Valeria, 98125, Messina, Italy
    Arch Biochem Biophys 403:149-54. 2002
    ....
  26. ncbi Ligand binding properties and structural studies of recombinant and chemically modified hemoglobins altered at beta 93 cysteine
    Yi Cheng
    Department of Biological Sciences, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213, USA
    Biochemistry 41:11901-13. 2002
    ....
  27. ncbi O-raffinose crosslinked hemoglobin lacks site-specific chemistry in the central cavity: structural and functional consequences of beta93Cys modification
    Robert A Boykins
    Laboratory of Biophysics, Division of Bacterial, Parasitic and Allergenic Products, Bethesda, Maryland 20892, USA
    Proteins 59:840-55. 2005
    ..Structural data presented here when taken together with the oxidative instability of O-R-PolyHbA0 may provide some basis for the reported toxicity of this oxygen carrier...
  28. ncbi Blood volume assessment with hemoglobin subtype analysis in preterm infants
    Jaana A Leipälä
    Hospital for Children and Adolescents, University of Helsinki, Finland
    Biol Neonate 84:41-4. 2003
    ..6 ml/kg, respectively. There was a close correlation between the RCVs measured with the two techniques (Pearson correlation 0.83, p < 0.05). In conclusion, HbST provides a reliable and safe determination of RCV in preterm infants...
  29. ncbi Human erythrocyte membrane band 3 protein influences hemoglobin cooperativity. Possible effect on oxygen transport
    Yuxun Zhang
    Department of Biology, Northeastern University, Boston, Massachusetts 02115, USA
    J Biol Chem 278:39565-71. 2003
    ..Membrane modulation of hemoglobin oxygen affinity has particularly interesting implications for the polymerization of hemoglobin S in the sickle red cell...
  30. ncbi Steady-state plasma lactoferrin levels in relation to infections and complications of sickle cell disease
    Iheanyi Okpala
    Hematol J 5:371-2. 2004
  31. ncbi Significance of beta116 His (G18) at alpha1beta1 contact sites for alphabeta assembly and autoxidation of hemoglobin
    Kazuhiko Adachi
    The Children s Hospital of Philadelphia, Division of Hematology and NMR Core Facility, Philadelphia, Pennsylvania 19104, USA
    Biochemistry 42:10252-9. 2003
    ..Tertiary structural changes may lead to changes in the heme region in beta chains after assembly with alpha chains, which could influence stability of dioxygen binding of beta chains...
  32. ncbi Spectroscopic and functional characterization of T state hemoglobin conformations encapsulated in silica gels
    Uri Samuni
    Physiology and Biophysics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Ullman 303, Bronx, New York 10461, USA
    Biochemistry 43:13674-82. 2004
    ..Thus, it appears that the encapsulation protocol-dependent rate of ligand-binding-induced relaxation determines the functional properties of the initially encapsulated deoxy-HbA population...
  33. ncbi Evidence for a metabolic shift of arginine metabolism in sickle cell disease
    John John B Schnog
    Department of Internal Medicine 9B, Slotervaart Hospital, Louwesweg 6, 1066 EC Amsterdam, The Netherlands
    Ann Hematol 83:371-5. 2004
    ....
  34. ncbi Platelet distribution width (PDW) is increased in vaso-occlusive crisis in sickle cell disease
    Manik A Amin
    Lata Medical Research Foundation, Nagpur, India
    Ann Hematol 83:331-5. 2004
    ..Our findings bridge previous inconsistencies relating to the role of platelets in sickle cell disease. Implications of this finding are discussed...
  35. ncbi Oxygen dependence of K(+)-Cl- cotransport in human red cell ghosts and sickle cells
    Asif I Khan
    University Laboratory of Physiology, Parks Road, Oxford OX1 3PT, UK
    Bioelectrochemistry 62:141-6. 2004
    ..These preparations provide a useful system with which to study the components involved in O2-sensitive membrane transport and why it is perturbed in certain pathological conditions (such as sickle cell disease and oxidant toxicity)...
  36. ncbi Arterial stiffness and wave reflections in patients with sickle cell disease
    Daniel Lemogoum
    Hypertension Clinic, Department of Cardiology, Erasme Hospital, 808 Lennik Road, 1070 Brussels, Belgium
    Hypertension 44:924-9. 2004
    ..SS patients have a paradoxical negative association between PWV and MAP, suggesting that low MAP does not protect them against arterial stiffness impairment...
  37. ncbi Hepcidin, haemoglobin and ferritin levels in sickle cell anaemia
    Chukwuma Ezeh
    Eur J Haematol 74:86-8. 2005
  38. pmc Relationship of binding of immunoglobulin G to Plasmodium falciparum-infected erythrocytes with parasite endemicity and antibody responses to conserved antigen in immune individuals
    Antoine Marie Diatta
    Unite d immunologie, Institut Pasteur de Dakar, Dakar, Senegal
    Clin Diagn Lab Immunol 11:6-11. 2004
    ..Measures of IgG binding to live IRBC are thus relevant for the functional screening of conserved IRBC-associated Ags that contribute to parasite destruction in vivo, as these Ags might be included in a multitarget vaccine...
  39. ncbi Expression of HbC and HbS, but not HbA, results in activation of K-Cl cotransport activity in transgenic mouse red cells
    Jose R Romero
    Division of Endocrinology, Diabetes and Hypertension, Harvard Medical School, Boston, MA, USA
    Blood 103:2384-90. 2004
    ..We conclude that human globins are able to stimulate the activity and/or regulation of K-Cl cotransport in mouse red cells. These observations support the contention that HbS and HbC stimulate K-Cl cotransport in human red cells...
  40. ncbi Arginine supplementation improves rotorod performance in sickle transgenic mice
    Francisca R Fasipe
    Division of Pediatric Hematology and Oncology, Department of Pediatrics, Montefiore Medical Centre, Albert Einstein College of Medicine, Bronx, NY 10467, USA
    Hematology 9:301-5. 2004
    ..In conclusion, impaired rotorod performance in sickle transgenic mice presents an opportunity to apply this simple task to provide an efficient method to screen some types of therapeutic regimens for efficacy in SCD...
  41. ncbi Allosteric effect of water in fish and human hemoglobins
    Christian Hundahl
    Department of Zoophysiology, Institute of Biological Sciences, C F Møllers Alle 131, University of Aarhus, DK 8000 Aarhus C, Denmark
    J Biol Chem 278:42769-73. 2003
    ..effects on the oxygen affinity of fish (trout I) hemoglobin that questioned allosteric water binding in human hemoglobin A (Bellelli, A., Brancaccio, A., and Brunori, M. (1993) J. Biol. Chem...
  42. ncbi Effect of Plasmodium falciparum parasitaemia on some haematological parameters in adolescent and adult Nigerian HbAA and HbAS blood genotypes
    M U Eteng
    Department of Biochemistry, College of Medical Sciences, University of Calabar, P M B 1115, Calabar, Cross Rivers State, Nigeria
    Cent Afr J Med 48:129-32. 2002
    ..To compare the susceptibility of HbAA and HbAS blood genotypes to Plasmodium falciparum in human subjects...
  43. ncbi The effect of adult hemoglobin on red blood cell nitric oxide levels during fetal development
    Harry Bard
    Pediatric Department, Sainte Justine s Hospital, University of Montreal, Montreal, Canada
    Biol Neonate 87:203-6. 2005
    ..To compare the levels of S-nitrosohemoglobin (HbSNO) at different gestational ages in newborn infants and correlate the levels of HbSNO with HbA and HbF...
  44. ncbi Oxidative reactions of normal and abnormal hemoglobins in the presence of phosphatidylserine vesicles
    Merita Nirmali Dias Wickramaratne
    Department of Chemistry, Loyola University of Chicago, Chicago, Illinois, USA
    Hemoglobin 29:27-42. 2005
    ..These findings suggest that heme oxidation and related reactions may not be responsible for detrimental cellular events in sickle erythrocytes under physiological conditions, as suggested in the literature...
  45. ncbi New insights into the proton-dependent oxygen affinity of Root effect haemoglobins
    C Bonaventura
    Nicholas School of the Environment and Earth Science, Duke University Marine Laboratory, Beaufort, NC 28516, USA
    Acta Physiol Scand 182:245-58. 2004
    ..Allosteric effectors moderate these steric effects within both R- and T-state conformations and allow for an elegant match between Hb function and the wide-ranging physiological needs of diverse organisms...
  46. ncbi Structure-function relationships in hemoglobin Kariya, Lys-40(C5) alpha----Glu, with high oxygen affinity. Functional role of the salt bridge between Lys-40 alpha and the beta chain COOH terminus
    K Imai
    Department of Physiochemical Physiology, Medical School, Osaka University, Japan
    J Biol Chem 264:11174-80. 1989
    ..The nature of another salt bridge between Asp-94 beta and the COOH-terminal His of beta chain was also discussed in comparison with the salt bridge involving Lys-40 alpha...
  47. ncbi Hydroxyurea-induced oxidative damage of normal and sickle cell hemoglobins in vitro: amelioration by radical scavengers
    E W Iyamu
    Comprehensive Sickle Cell Center, Meharry Medical College, Nashville, Tennessee, USA
    J Clin Lab Anal 15:1-7. 2001
    ..These results indicate that the HU-induced damage of hemoglobin and their component globin chains can be reduced by radical scavengers...
  48. doi Unexpected hemoglobin A results after an erythrocyte exchange: importance of specimen mixing
    Megan E Sullivan
    Clin Chem 54:1088-90. 2008
  49. ncbi Three new beta-globin gene promoter mutations identified through newborn screening
    Barry Eng
    Hamilton Regional Laboratory Medicine Program, Hamilton Health Sciences, Hamilton, Ontario, Canada
    Hemoglobin 31:129-34. 2007
    ..Two cases involved single base substitutions at positions -25 (G-->C) and -32 (C-->T). The remaining case involved the deletion of two bases (-AA) at positions -27 and -26...
  50. pmc Insights into the solution structure of human deoxyhemoglobin in the absence and presence of an allosteric effector
    Sarata C Sahu
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA
    Biochemistry 46:9973-80. 2007
    ..These new structural findings allow us to gain a deeper insight into the structure-function relationship of this interesting allosteric protein...
  51. ncbi Nitrite reductase activity of hemoglobin S (sickle) provides insight into contributions of heme redox potential versus ligand affinity
    Rozalina Grubina
    Vascular Medicine Branch, NHLBI, National Institutes of Health, Bethesda, MD 20892, USA
    J Biol Chem 283:3628-38. 2008
    b>Hemoglobin A (HbA) is an allosterically regulated nitrite reductase that reduces nitrite to NO under physiological hypoxia...
  52. ncbi Treatment of sickle cell anemia mouse model with iPS cells generated from autologous skin
    Jacob Hanna
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Science 318:1920-3. 2007
    ..The problems associated with using retroviruses and oncogenes for reprogramming need to be resolved before iPS cells can be considered for human therapy...
  53. doi Reduction of hypervalent states of myoglobin and hemoglobin to their ferrous forms by thymoquinone: the role of GSH, NADH and NADPH
    K H Khalife
    Department of Molecular, Cellular, and Animal Biology, University of Camerino, Via Camerini 2, 62032 Camerino MC Italy
    Biochim Biophys Acta 1780:627-37. 2008
    ..This is of particular biological significance, as natural quinones may participate in reducing processes that lead to recovery of hemoglobin and myoglobin during oxidative stress...
  54. ncbi Ion-exchange high-performance liquid chromatographic separation of protein variants and isoforms on MCI GEL ProtEx stationary phases
    T Adachi
    Yokohama Research Center, Mitsubishi Chemical Corporation, Japan
    J Chromatogr A 763:57-63. 1997
    ..These characteristics of ProtEx stationary phases may be attributed both to particle uniformity and to hydrophillic surface coverage of the base polymeric material...
  55. ncbi Crystal structure of T state haemoglobin with oxygen bound at all four haems
    M Paoli
    Department of Chemistry, University of York, Heslington, York, UK
    J Mol Biol 256:775-92. 1996
    ..They provide clear evidence of haem-haem interaction in the T state...
  56. ncbi Separation of hemoglobin variants in single human erythrocytes by capillary electrophoresis with laser-induced native fluorescence detection
    S J Lillard
    Department of Chemistry, Iowa State University, Ames 50011, USA
    J Chromatogr A 718:397-404. 1995
    ..Normal adult cells contained 9.6% and 4.8% glycated beta- and alpha-chains, respectively. Cells with elevated Hb A1 gave 30% and 12%, respectively. The amounts of glycated Hb and total Hb in a given cell were found to be uncorrelated...
  57. ncbi Haemoglobin caribbean beta91 (F7) Leu replaced by Arg: a mildly haemoglobin with a low oxygen affinity
    E Ahern
    FEBS Lett 69:99-102. 1976
  58. ncbi Role of hepatic enzymes in the biochemical assessment of the severity of sickle cell anemia
    D P Oparinde
    Department of Chemical Pathology and Immunology, Ladoke Akintola University of Technology, College of Medicine, University of Ilorin, florin, Kwara State, Nigeria
    Trop Gastroenterol 27:118-21. 2006
    ....
  59. ncbi The Bohr effect of hemoglobin intermediates and the role of salt bridges in the tertiary/quaternary transitions
    R Russo
    Dipartimento di Scienze e Tecnologie Biomediche, , Laboratori Interdisciplinari Tecnologie Avanzante 20090 Segrate, Italy
    J Biol Chem 276:13628-34. 2001
    ..The rupture of the salt bridges per se does not drive the transition...
  60. ncbi Distal ligand reactivity and quaternary structure studies of proximally detached hemoglobins
    D Barrick
    T. C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore Maryland 21218, USA
    Biochemistry 40:3780-95. 2001
    ..Some residual allosteric interactions may remain operative at the beta-chains of rHb (alphaH87G)...
  61. pmc Correction of the sickle cell mutation in embryonic stem cells
    Judy C Chang
    Department of Laboratory Medicine, Cardiovascular Research Institute and Center of Human Genetics, University of California, San Francisco, CA 94143, USA
    Proc Natl Acad Sci U S A 103:1036-40. 2006
    ..Hematopoietic cells differentiated from these ES cells produced both hemoglobin A and hemoglobin S...
  62. ncbi Dehydration response of sickle cells to sickling-induced Ca(++) permeabilization
    Virgilio L Lew
    Physiological Laboratory, University of Cambridge, United Kingdom
    Blood 99:2578-85. 2002
    ..A higher participation of SS reticulocytes in P(sickle)-triggered dehydration suggests that they form these pathways more efficiently than discocytes despite their lower cell hemoglobin concentrations...
  63. ncbi Assembly of human hemoglobin (Hb) beta- and gamma-globin chains expressed in a cell-free system with alpha-globin chains to form Hb A and Hb F
    Kazuhiko Adachi
    Children s Hospital of Philadelphia, Division of Hematology and the University of Pennsylvania School of Medicine Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 277:13415-20. 2002
    ....
  64. ncbi The interaction of nitric oxide with distinct hemoglobins differentially amplifies endothelial heme uptake and heme oxygenase-1 expression
    Roberta Foresti
    Vascular Biology Unit, Department of Surgical Research, Northwick Park Institute for Medical Research, Watford Road, Harrow, Middlesex, HA1 3UJ, UK
    J Pharmacol Exp Ther 317:1125-33. 2006
    ..In conclusion, our data indicate a novel role for NO in the modulation of heme transport and HO-1 induction in endothelial cells, which may be relevant for hematological disorders characterized by disruption of the heme-NO equilibrium...
  65. ncbi Sickle hemoglobin polymer stability probed by triple and quadruple mutant hybrids
    Xianfeng Li
    Department of Biochemistry, Northeastern University, Boston, Massachusetts 02115, USA
    J Biol Chem 277:13479-87. 2002
    ..5 g/dl. A second way to explain these data is by the modification of motional freedom, thereby changing vibrational entropy in the polymer...
  66. ncbi Premature neonate with apnea and trace amounts of hemoglobin S and A detected by high-performance liquid chromatography. Sickle cell trait/disease in a prematurely born baby with hampered switch from gamma-globin to beta-globin chain synthesis
    Maria Uzquiano
    Department of Pathology and Laboratory Medicine, University of Texas Health Science Center, Houston Medical School, Houston, TX 77030, USA
    Arch Pathol Lab Med 130:733-4. 2006
  67. ncbi [Application of Helena SAS-3 full automatic hemoglobin agarose electrophoresis]
    Jian kun Mo
    Clinical Laboratory, Second People s Hospital of Guangdong Province, Guangzhou 510310, China
    Di Yi Jun Yi Da Xue Xue Bao 25:1565-7. 2005
    ..To assess the value of Halena SAS-3 full automatic hemoglobin agarose electrophoresis in the diagnosis of thalassemia...
  68. ncbi Role of redox potential of hemoglobin-based oxygen carriers on methemoglobin reduction by plasma components
    Scott C Dorman
    Department of Chemistry, University of South Alabama, Mobile, USA
    Artif Cells Blood Substit Immobil Biotechnol 30:39-51. 2002
    ..HbA, HbXL99alpha, and oxyglobin were only partially reduced (10 to 37%) by glutathione, beta-NADH, and ascorbic acid under similar conditions...
  69. pmc The effect of deoxygenation on whole-cell conductance of red blood cells from healthy individuals and patients with sickle cell disease
    Joseph A Browning
    Department of Physiology, Anatomy and Genetics, University of Oxford, United Kingdom
    Blood 109:2622-9. 2007
    ..These findings represent the first electrical recordings of single HbS cells and will facilitate progress in understanding altered red blood cell cation transport characteristics of SCD...
  70. ncbi Effects of hydroxyurea and L-arginine on the production of nitric oxide metabolites in cultures of normal and sickle erythrocytes
    Masoud Nahavandi
    Departments of Anesthesiology, Howard University College of Medicine, Washington, DC 20059, USA
    Hematology 11:291-4. 2006
    ..Therefore, we conclude that a therapeutic dose of HU may increase the plasma concentration of NO by a mechanism that does not require erythrocytes NOS activity...
  71. pmc Haem disorder in recombinant- and reticulocyte-derived haemoglobins: evidence for stereoselective haem insertion in eukaryotes
    A J Mathews
    School of Biological Sciences, University of Auckland, 3 Symonds Street, Auckland, New Zealand
    Biochem J 357:305-11. 2001
    ..We propose that, in eukaryotes, in vivo haem insertion occurs via both co-translational mechanisms and insertion via semiHb-beta...
  72. doi Effects of gliclazide on platelet aggregation and the plasminogen activator inhibitor type 1 level in patients with type 2 diabetes mellitus
    Hiroyuki Konya
    Division of Diabetes and Metabolism, Department of Internal Medicine, Hyogo College of Medicine, Nishinomiya, Hyogo 663 8501, Japan
    Metabolism 59:1294-9. 2010
    ..mass index, fasting plasma glucose, immunoreactive insulin, homeostasis model assessment of insulin resistance, hemoglobin A(1c) (HbA(1c)), total cholesterol, triglycerides, high-density lipoprotein cholesterol, prothrombin time, ..
  73. doi C-peptide levels and insulin independence following autologous nonmyeloablative hematopoietic stem cell transplantation in newly diagnosed type 1 diabetes mellitus
    Carlos E B Couri
    Department of Clinical Medicine, School of Medicine of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Brazil
    JAMA 301:1573-9. 2009
    ..Most patients became insulin free with normal levels of glycated hemoglobin A(1c) (HbA(1c)) during a mean 18.8-month follow-up...
  74. ncbi Emphysematous pyelonephritis: clinicoradiological classification, management, prognosis, and pathogenesis
    J J Huang
    Department of Internal Medicine, National Cheng Kung University Hospital, Tainan, Taiwan, Republic of China
    Arch Intern Med 160:797-805. 2000
    ..The radiological classification and adequate therapeutic regimen are controversial and the prognostic factors and pathogenesis remain uncertain...
  75. pmc Nicotinamide effects oxidative burst activity of neutrophils in patients with poorly controlled type 2 diabetes mellitus
    Zeynep Osar
    Department of Internal Medicine, Division of Endocrinology, Metabolism, and Diabetes, Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey
    Exp Diabesity Res 5:155-62. 2004
    ..All patients were on sulphonylurea treatment and their hemoglobin A(1c) (HbA(1c)) levels were above 7.5%. The control group consisted of 10 voluntary healthy subjects...
  76. ncbi Proteinuria, a target for renoprotection in patients with type 2 diabetic nephropathy: lessons from RENAAL
    Dick de Zeeuw
    Department of Clinical Pharmacology, University Medical Center Groningen, Groningen, The Netherlands
    Kidney Int 65:2309-20. 2004
    ....
  77. doi Addition of metformin to exogenous glucagon-like peptide-1 results in increased serum glucagon-like peptide-1 concentrations and greater glucose lowering in type 2 diabetes mellitus
    Joy Cuthbertson
    Regional Centre for Endocrinology and Diabetes, Royal Victoria Hospital, Belfast, BT126BA Northern Ireland, UK
    Metabolism 60:52-6. 2011
    ..7 ± 2.6 years [mean ± SEM]; body mass index, 29.6 ± 1.7 kg/m²; hemoglobin A(1c), 7.0% ± 0...
  78. ncbi Three-year follow-up study of blood-retinal barrier and retinal thickness alterations in patients with type 2 diabetes mellitus and mild nonproliferative diabetic retinopathy
    Conceição L Lobo
    Centre of Ophthalmology, University Hospital and Institute of Biomedical Research on Light and Image, Faculty of Medicine, University of Coimbra, Coimbra, Portugal
    Arch Ophthalmol 122:211-7. 2004
    ..To examine the 3-year alterations of the blood-retinal barrier and changes in retinal thickness occurring in the macular region in 14 eyes of 14 patients with type 2 diabetes mellitus (DM) and mild nonproliferative diabetic retinopathy...
  79. ncbi Nitric oxide modulates the activity of the hemoproteins prostaglandin I2 synthase and thromboxane A2 synthase
    M L Wade
    Huntsman Cancer Institute, University of Utah, Salt Lake City, Utah 84112, USA
    Arch Biochem Biophys 347:174-80. 1997
    ..We conclude that nitric oxide can modulate certain hemoprotein enzymes in the biosynthetic cascade that governs the formation of eicosanoid mediators of thrombosis and hemostasis...
  80. ncbi Crystal structures of HbA2 and HbE and modeling of hemoglobin delta 4: interpretation of the thermal stability and the antisickling effect of HbA2 and identification of the ferrocyanide binding site in Hb
    Udayaditya Sen
    Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, 1 AF Bidhan Nagar, Kolkata 700064, India
    Biochemistry 43:12477-88. 2004
    b>Hemoglobin A(2) (alpha(2)delta(2)) is an important hemoglobin variant which is a minor component (2-3%) in the circulating red blood cells, and its elevated concentration in beta-thalassemia is a useful clinical diagnostic...
  81. doi Analysis of major protein-protein and protein-metal complexes of erythrocytes directly from cell lysate utilizing capillary electrophoresis mass spectrometry
    An Nguyen
    Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, Texas 78712, USA
    Anal Chem 80:7169-73. 2008
    ..zinc cofactor, carbonic anhydrase I (CAI-Zn at approximately 7 amol/cell) complexed with its zinc cofactor, and hemoglobin A (Hb-tetramer at approximately 450 amol/cell)a tetramer formed by two alpha-beta-subunits and four heme groups...
  82. ncbi Effects of race and family history of type 2 diabetes on metabolic status of women with polycystic ovary syndrome
    David A Ehrmann
    Department of Medicine, University of Chicago Medical Center, Chicago, Illinois 60637, USA
    J Clin Endocrinol Metab 90:66-71. 2005
    ..homeostasis model assessment model of insulin resistance derived from fasting levels of glucose and insulin), hemoglobin A(1c), and SHBG, and dehydroepiandrosterone sulfate levels...
  83. ncbi [Use of continuous subcutaneous insulin infusion by a portable insulin pump during pregnancy in women with type 1 diabetes mellitus]
    Svetlana Zoric
    Klinicki centar Srbije, Institut za endokrinologiju, dijabetes i bolesti metabolizma, Beograd
    Vojnosanit Pregl 63:648-51. 2006
    ....
  84. ncbi Increased serum glycated albumin level is associated with the presence and severity of coronary artery disease in type 2 diabetic patients
    Li Jin Pu
    Department of Cardiology, Rui Jin Hospital, Institute of Cardiovascular Diseases, Jiaotong University School of Medicine, Shanghai, People s Republic of China
    Circ J 71:1067-73. 2007
    ..The aim of this study was to assess the relationship between increased serum glycated albumin level and the presence and severity of coronary artery disease (CAD) in patients with type 2 diabetes mellitus (T2DM)...
  85. doi Cardiovascular and metabolic effects of medroxyprogesterone acetate versus conjugated equine estrogen after premenopausal hysterectomy with bilateral ovariectomy
    Shirin Kalyan
    Centre for Menstrual Cycle and Ovulation Research, University of British Columbia, Vancouver, British Columbia V5Z 1M9, Canada
    Pharmacotherapy 30:442-52. 2010
    ..To compare the cardiovascular and metabolic effects of medroxyprogesterone acetate (MPA) with those of conjugated equine estrogen (CEE) as single-hormone therapies in women who underwent hysterectomy with bilateral ovariectomy...
  86. pmc A longitudinal study of risk factors for incident albuminuria in diabetic American Indians: the Strong Heart Study
    Jiaqiong Xu
    Center for American Indian Health Research, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA
    Am J Kidney Dis 51:415-24. 2008
    ..There have been no studies that use longitudinal data with more than 2 measurements and methods of longitudinal data analysis to identify risk factors for incident albuminuria over time more effectively...
  87. doi Transgenic human alpha-hemoglobin stabilizing protein could partially relieve betaIVS-2-654-thalassemia syndrome in model mice
    Baobin Wang
    Shanghai Institute of Medical Genetics, Shanghai Children s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People s Republic of China
    Hum Gene Ther 21:149-56. 2010
    ..a chaperone protein for proper folding and stabilization of free alphaHb in red blood cells, thus aiding hemoglobin A (HbA) assembly, could relieve the pathogenic state of red blood cells in beta-thalassemia...
  88. ncbi Improved human pancreatic islet isolation for a prospective cohort study of islet transplantation vs best medical therapy in type 1 diabetes mellitus
    Garth L Warnock
    Department of Surgery, British Columbia Islet Transplant Centre, University of British Columbia, 950 W 10th Avenue, Vancouver, BC, Canada V5Z 4E3
    Arch Surg 140:735-44. 2005
    ..A local multiorgan donor pancreas procurement program can provide a source for optimized isolation of purified viable islets for transplantation into patients with type 1 diabetes mellitus receiving best medical therapy...
  89. ncbi Fetal hemoglobin modulation during human erythropoiesis: stem cell factor has "late" effects related to the expression pattern of CD117
    Urszula Wojda
    Laboratory of Chemical Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 101:492-7. 2003
    ..The average HbF/hemoglobin A (HbA) ratio was 30.9% +/- 18.7% in cultures containing SCF compared with 4.1% +/- 2...
  90. ncbi Sibutramine effect on metabolic control of obese patients with type 2 diabetes mellitus treated with pioglitazone
    Giuseppe Derosa
    Department of Internal Medicine and Therapeutics, University of Pavia, 19 27100 Pavia, Italy
    Metabolism 57:1552-7. 2008
    ..We assessed body mass index, hemoglobin A(1c) (HbA(1c)), fasting plasma glucose (FPG), postprandial plasma glucose (PPG), fasting plasma insulin (FPI), ..
  91. ncbi Clinical impact of a pharmacist-managed diabetes mellitus drug therapy management service
    Amie D McCord
    From the Department of Pharmacy Practice, Chicago College of Pharmacy, Midwestern University, Downers Grove, Illinois 60515, USA
    Pharmacotherapy 26:248-53. 2006
    ..To evaluate the impact of clinical pharmacist interventions, including drug therapy management, on outcomes relevant to diabetes mellitus...
  92. ncbi Addition of neutral protamine lispro insulin or insulin glargine to oral type 2 diabetes regimens for patients with suboptimal glycemic control: a randomized trial
    Katherine Esposito
    Second University of Naples and Azienda Sanitaria Locale Napoli 5, Naples, Italy
    Ann Intern Med 149:531-9. 2008
    ..Neutral protamine lispro (NPL) insulin has demonstrated better glycemic control and similar incidence of hypoglycemic events than that of neutral protamine Hagedorn insulin...
  93. ncbi Nature of the FeO2 bonding in myoglobin and hemoglobin: A new molecular paradigm
    Keiji Shikama
    Biological Institute, Graduate School of Life Sciences, Tohoku University, Sendai, Japan
    Prog Biophys Mol Biol 91:83-162. 2006
    ....
  94. doi Glucose control and walking in a multiethnic sample of older adults
    Ann M Swartz
    Department of Human Movement Sciences, University of Wisconsin Milwaukee, Milwaukee, Wisc, USA
    Gerontology 53:454-61. 2007
    ..Although walking is the most commonly reported physical activity by older adults, there is a paucity of data determining the relationship between objectively determined walking behavior and glucose dynamics in older adults...
  95. ncbi Effect of pioglitazone on body composition and energy expenditure: a randomized controlled trial
    Steven R Smith
    Department of Molecular Endocrinology, Pennington Biomedical Research Center, Baton Rouge, LA 70808, USA
    Metabolism 54:24-32. 2005
    ..Some, but not all, studies demonstrate reductions in visceral adipose tissue. Changes in body weight are the result of changes in energy intake, energy expenditure, or both...
  96. doi Class effect of erythropoietin therapy on hemoglobin A(1c) in a patient with diabetes mellitus and chronic kidney disease not undergoing hemodialysis
    Jamie N Brown
    Department of Pharmacy, Durham Veterans Affairs Medical Center, Durham, North Carolina 27705, USA
    Pharmacotherapy 29:468-72. 2009
    In patients with diabetes mellitus, hemoglobin A(1c) (A1C) is commonly interpreted as a measure of long-term glycemic control, reflecting a mean glucose level over the previous 2-3 months...
  97. doi Effect of noninsulin antidiabetic drugs added to metformin therapy on glycemic control, weight gain, and hypoglycemia in type 2 diabetes
    Olivia J Phung
    University of Connecticut School of Pharmacy, Storrs, and Drug Information Center, Hartford Hospital, Hartford, Connecticut 06102 5037, USA
    JAMA 303:1410-8. 2010
    ..Metformin is the recommended initial drug therapy for patients with type 2 diabetes mellitus (DM). However, the optimal second-line drug when metformin monotherapy fails is unclear...
  98. ncbi Progression of diabetic nephropathy
    P Hovind
    Steno Diabetes Center, Gentofte, Denmark
    Kidney Int 59:702-9. 2001
    ..The risk factors of losing filtration power (progression promoters) have not been clearly identified. Furthermore, information on optimal arterial blood pressure, glycemic control, and cholesterol levels are lacking...
  99. ncbi Effects of health maintenance organization coverage of self-monitoring devices on diabetes self-care and glycemic control
    Stephen B Soumerai
    Department of Ambulatory Care and Prevention, Harvard Medical School and Harvard Pilgrim Health Care, Boston, MA 02215, USA
    Arch Intern Med 164:645-52. 2004
    ..We evaluated whether a policy providing free blood glucose monitors increased SMBG and whether initiating SMBG was associated with increased regularity of medication use and improved glucose control (hemoglobin A(1c) [HbA(1c)] level).
  100. pmc Refill adherence to oral hypoglycemic agents and glycemic control in veterans
    Nancy Kim
    Department of Internal Medicine, Section of General Internal Medicine, School of Medicine, Yale University, Veterans Affairs Connecticut Healthcare System, West Haven, CT 06516, USA
    Ann Pharmacother 44:800-8. 2010
    ..Persistence, the continuous refill of medications, is one metric that could be integrated into clinical care if associated with glycemic control...
  101. ncbi Effect of improved glycemic control on health care costs and utilization
    E H Wagner
    MacColl Institute for Healthcare Innovation, Center for Health Studies, Group Health Cooperative, 1730 Minor Ave, Suite 1290, Seattle, WA 98101, USA
    JAMA 285:182-9. 2001
    ..Because of the additional costs associated with improving diabetes management, there is interest in whether improved glycemic control leads to reductions in health care costs, and, if so, when such cost savings occur...

Research Grants73

  1. OXYGEN TRANSPORT CYS CELL FREE HEMOGLOBIN
    Robert Winslow; Fiscal Year: 1999
    ..Taken together, these aims will not only lead to rationally-designed oxygen carriers for clinical use, but also important insight into mechanisms of vasoactivity and control of tissue oxygenation. ..
  2. OXYGEN TRANSPORT CYS CELL FREE HEMOGLOBIN
    Robert Winslow; Fiscal Year: 2000
    ..Taken together, these aims will not only lead to rationally-designed oxygen carriers for clinical use, but also important insight into mechanisms of vasoactivity and control of tissue oxygenation. ..
  3. OXYGEN TRANSPORT CYS CELL FREE HEMOGLOBIN
    Robert Winslow; Fiscal Year: 2001
    ..Taken together, these aims will not only lead to rationally-designed oxygen carriers for clinical use, but also important insight into mechanisms of vasoactivity and control of tissue oxygenation. ..
  4. OXYGEN TRANSPORT CYS CELL FREE HEMOGLOBIN
    Robert Winslow; Fiscal Year: 2002
    ..Taken together, these aims will not only lead to rationally-designed oxygen carriers for clinical use, but also important insight into mechanisms of vasoactivity and control of tissue oxygenation. ..
  5. Heme Oxygenase and Spinal Cord Injury
    Linda Noble; Fiscal Year: 2004
    ..abstract_text> ..
  6. Heme Oxygenase and Spinal Cord Injury
    Linda Noble; Fiscal Year: 2002
    ..abstract_text> ..
  7. Heme Oxygenase and Spinal Cord Injury
    Linda Noble; Fiscal Year: 2001
    ..abstract_text> ..
  8. Heme Oxygenase and Spinal Cord Injury
    Linda Noble; Fiscal Year: 2003
    ..abstract_text> ..
  9. METALLOPROTEIN NITROSYL FORMATION AND REACTIVITY
    Michael Doyle; Fiscal Year: 1980
    ..Selective intermolecular transfer of the nitrosyl ligand in Co(NO)D2 and (CoCq(NO)(en)2)C104 to hemoglobin A and myoglobin has been observed...
  10. Role of nitrite and hemoglobin in vascular NO homeostasis in the fetus and adult
    Arlin B Blood; Fiscal Year: 2010
    ..If so, nitrite holds wide- ranging potential as a therapeutic agent for the treatment of diseases and injury involving hypoxic/ischemic stress. ..
  11. Role of nitrite and hemoglobin in vascular NO homeostasis in the fetus and adult
    ARLIN BLOOD; Fiscal Year: 2009
    ..If so, nitrite holds wide- ranging potential as a therapeutic agent for the treatment of diseases and injury involving hypoxic/ischemic stress. ..
  12. Characterization of a alpha-globin chaperone protein
    Mitchell Weiss; Fiscal Year: 2003
    ..EDRF interacts with free alpha-globin but not with beta-globin or hemoglobin A (alpha2beta2)...
  13. Characterization of a alpha-globin chaperone protein
    Mitchell Weiss; Fiscal Year: 2003
    ..EDRF interacts with free alpha-globin but not with beta-globin or hemoglobin A (alpha2beta2)...
  14. Characterization of a alpha-globin chaperone protein
    Mitchell Weiss; Fiscal Year: 2005
    ..EDRF interacts with free alpha-globin but not with beta-globin or hemoglobin A (alpha2beta2)...
  15. Characterization of a alpha-globin chaperone protein
    Mitchell Weiss; Fiscal Year: 2003
    ..EDRF interacts with free alpha-globin but not with beta-globin or hemoglobin A (alpha2beta2)...
  16. Characterization of a alpha-globin chaperone protein
    Mitchell Weiss; Fiscal Year: 2006
    ..EDRF interacts with free alpha-globin but not with beta-globin or hemoglobin A (alpha2beta2)...
  17. Characterization of a alpha-globin chaperone protein
    Mitchell Weiss; Fiscal Year: 2004
    ..EDRF interacts with free alpha-globin but not with beta-globin or hemoglobin A (alpha2beta2)...
  18. BAND 3 N-TERMINAL AA (10-16) AND DEOXY S POLYMERIZATION
    John Harris; Fiscal Year: 1990
    ..the amino acid sequence present in the N-terminal portion of Band 3 of the human erythrocyte bind reversibly to hemoglobin A at the 2,3-DPG receptor locus. Binding to the deoxy conformation is much greater than to the oxy...
  19. Characterization of a alpha-globin chaperone protein
    Mitchell Weiss; Fiscal Year: 2002
    ..EDRF interacts with free alpha-globin but not with beta-globin or hemoglobin A (alpha2beta2)...
  20. Hemolysis, Lipid Oxidation and Vascular Dysfunction in Sickle Cell Disease
    TIMOTHY CHARLES FLEWELEN; Fiscal Year: 2010
    ..When completed, this research should lead to the discovery and exploitation of a new therapeutic target to aid in the treatment of sickle cell disease. ..
  21. Hemolysis, Lipid Oxidation and Vascular Dysfunction in Sickle Cell Disease
    TIMOTHY FLEWELEN; Fiscal Year: 2009
    ..When completed, this research should lead to the discovery and exploitation of a new therapeutic target to aid in the treatment of sickle cell disease. ..
  22. Regulation of insulator function and globin gene expression by USF and associated
    Suming Huang; Fiscal Year: 2010
    ..The studies will provide a novel insight into USF function in the regulation of developmental stage-specific globin gene expression and will lead to novel strategies for the molecular therapy of anemia. ..
  23. Regulation of insulator function and globin gene expression by USF and associated
    Suming Huang; Fiscal Year: 2009
    ..The studies will provide a novel insight into USF function in the regulation of developmental stage-specific globin gene expression and will lead to novel strategies for the molecular therapy of anemia. ..
  24. BIOCHEMICAL BASIS OF THE COMPLICATIONS OF DIABETES
    Anthony Cerami; Fiscal Year: 1980
    ..Biological studies have shown that hemoglobin AIc is formed from hemoglobin A as a postsynthetic modification whose rate is dictated by the environment in which the red cells circulate...
  25. Regulation of insulator function and globin gene expression by USF and associated
    Suming Huang; Fiscal Year: 2009
    ..The studies will provide a novel insight into USF function in the regulation of developmental stage-specific globin gene expression and will lead to novel strategies for the molecular therapy of anemia. ..
  26. IN VITRO MODELS OF SICKLE CELL GENE THERAPY
    GEORGE ATWEH; Fiscal Year: 2001
    ..cell patient with a high level of fetal hemoglobin or a sickle beta-thalassemia patient with a high level of hemoglobin a where the normal or beta-globin chains replace some of the mutant sickle globin chains in the red cell...
  27. IN VITRO MODELS OF SICKLE CELL GENE THERAPY
    GEORGE ATWEH; Fiscal Year: 2002
    ..cell patient with a high level of fetal hemoglobin or a sickle beta-thalassemia patient with a high level of hemoglobin a where the normal or beta-globin chains replace some of the mutant sickle globin chains in the red cell...
  28. IN VITRO MODELS OF SICKLE CELL GENE THERAPY
    GEORGE ATWEH; Fiscal Year: 2002
    ..cell patient with a high level of fetal hemoglobin or a sickle beta-thalassemia patient with a high level of hemoglobin a where the normal or beta-globin chains replace some of the mutant sickle globin chains in the red cell...
  29. IN VITRO MODELS OF SICKLE CELL GENE THERAPY
    GEORGE ATWEH; Fiscal Year: 1999
    ..cell patient with a high level of fetal hemoglobin or a sickle beta-thalassemia patient with a high level of hemoglobin a where the normal or beta-globin chains replace some of the mutant sickle globin chains in the red cell...
  30. IN VITRO MODELS OF SICKLE CELL GENE THERAPY
    GEORGE ATWEH; Fiscal Year: 2000
    ..cell patient with a high level of fetal hemoglobin or a sickle beta-thalassemia patient with a high level of hemoglobin a where the normal or beta-globin chains replace some of the mutant sickle globin chains in the red cell...
  31. STRUCTURE AND INTERACTION IN SICKLE HEMOGLOBIN GELS
    ROBIN BRIEHL; Fiscal Year: 2004
    ..Energetics of the incorporation of hemoglobin A into the polymer, relevant to mutant site interactions, will be studied...
  32. STRUCTURE AND INTERACTION IN SICKLE HEMOGLOBIN GELS
    ROBIN BRIEHL; Fiscal Year: 2002
    ..Energetics of the incorporation of hemoglobin A into the polymer, relevant to mutant site interactions, will be studied...
  33. STRUCTURE-FUNCTION RELATIONSHIP OF HEMOGLOBIN A
    Ali Mansouri; Fiscal Year: 1980
    ..IHP must bring about some other subtle structural changes which affect the oxidation rate...
  34. BEHAVIORAL MEDICINE APPROACHES TO PEDIATRIC ACUTE PAIN
    KEITH SLIFER; Fiscal Year: 2004
    ..be stratified by child age, gender, baseline distress, length of time receiving injections, diabetes control hemoglobin A]c level at baseline) and parent years of education...
  35. BEHAVIORAL MEDICINE APPROACHES TO PEDIATRIC ACUTE PAIN
    KEITH SLIFER; Fiscal Year: 2002
    ..be stratified by child age, gender, baseline distress, length of time receiving injections, diabetes control hemoglobin A]c level at baseline) and parent years of education...
  36. BEHAVIORAL MEDICINE APPROACHES TO PEDIATRIC ACUTE PAIN
    KEITH SLIFER; Fiscal Year: 2003
    ..be stratified by child age, gender, baseline distress, length of time receiving injections, diabetes control hemoglobin A]c level at baseline) and parent years of education...
  37. HEMOGLOBIN--A WORKBENCH TO STUDY PROTEIN ALLOSTERY
    Arthur Arnone; Fiscal Year: 2004
    ..This research would result in new insights into hemoglobin's molecular mechanism that may provide for better understanding and treatment of hemoglobinopathies and for the design of improved blood substitutes. ..
  38. HEMOGLOBIN--A WORKBENCH TO STUDY PROTEIN ALLOSTERY
    Arthur Arnone; Fiscal Year: 1999
    ..This research would result in new insights into hemoglobin's molecular mechanism that may provide for better understanding and treatment of hemoglobinopathies and for the design of improved blood substitutes. ..
  39. HEMOGLOBIN--A WORKBENCH TO STUDY PROTEIN ALLOSTERY
    Arthur Arnone; Fiscal Year: 2003
    ..This research would result in new insights into hemoglobin's molecular mechanism that may provide for better understanding and treatment of hemoglobinopathies and for the design of improved blood substitutes. ..
  40. HEMOGLOBIN--A WORKBENCH TO STUDY PROTEIN ALLOSTERY
    Arthur Arnone; Fiscal Year: 2001
    ..This research would result in new insights into hemoglobin's molecular mechanism that may provide for better understanding and treatment of hemoglobinopathies and for the design of improved blood substitutes. ..
  41. HEMOGLOBIN--A WORKBENCH TO STUDY PROTEIN ALLOSTERY
    Arthur Arnone; Fiscal Year: 2000
    ..This research would result in new insights into hemoglobin's molecular mechanism that may provide for better understanding and treatment of hemoglobinopathies and for the design of improved blood substitutes. ..
  42. HEMOGLOBIN--A WORKBENCH TO STUDY PROTEIN ALLOSTERY
    Arthur Arnone; Fiscal Year: 2002
    ..This research would result in new insights into hemoglobin's molecular mechanism that may provide for better understanding and treatment of hemoglobinopathies and for the design of improved blood substitutes. ..
  43. MAPPING OF ELECTRON TUNNELING PATHWAYS IN PROTEINS
    David Beratan; Fiscal Year: 1993
    ..This proposal is intended to allow the continuation of collaborative studies by the co- investigators that led to the pathway concept and its resent numerical implementation...
  44. HEMEPROTEINS: STATIC & RAPID KINETIC MAGNETIC STUDIES
    Todd Schuster; Fiscal Year: 1980
    ..thermodynamic and kinetic linkages between changes in protein conformation and iron electronic configuration in hemoglobin A and S, other mutant hemoglobins and various metalloproteins...
  45. Lifestyle, insulin, IGF-I and colorectal cancer
    Rudolf Kaaks; Fiscal Year: 2004
    ..This study seeks to increase understanding of etiologic mechanisms relating over nutrition to colon cancer development, which will eventually allow the in formulation of more precise nutritional guidelines for efficient prevention. ..
  46. Lifestyle, insulin, IGF-I and colorectal cancer
    Rudolf Kaaks; Fiscal Year: 2003
    ..This study seeks to increase understanding of etiologic mechanisms relating over nutrition to colon cancer development, which will eventually allow the in formulation of more precise nutritional guidelines for efficient prevention. ..
  47. Lifestyle, insulin, IGF-I and colorectal cancer
    Rudolf Kaaks; Fiscal Year: 2005
    ..This study seeks to increase understanding of etiologic mechanisms relating over nutrition to colon cancer development, which will eventually allow the in formulation of more precise nutritional guidelines for efficient prevention. ..
  48. IMIDOESTERS AS ANTISICKLING AGENTS
    David Albert; Fiscal Year: 1991
    ..is to produce a stable HbS that will not aggregate upon deoxygenation and has an oxygen affinity similar to hemoglobin A in normal blood...
  49. SUBUNIT INTERACTIONS IN SICKLE CELL HEMOGLOBIN
    GARY ACKERS; Fiscal Year: 1980
    ..These data will provide for each hemoglobin a linkage map defining the variation of subunit interactions at each successive stage of oxygenation...
  50. MEMBRANE-BOUND HEMOGLOBIN AND SICKLE-CELL DISEASE
    MITCHEL SAYARE; Fiscal Year: 1980
    ..The specific membrane protein(s) responsible for this binding and the question of similarity of binding of hemoglobin A and S to this site will be established by further cross-linking studies as outlined in the proposal...
  51. STRUCTURE AND FUNCTION OF HEMOGLOBIN
    FUMITO TAKETA; Fiscal Year: 1980
    ..The mechanism of activation and inhibition, including non-equivalence in alpha and beta heme reactivity, is of interest. ..
  52. PHYSICAL STUDIES OF BIOLOGICAL MACROMOLECULES
    S Englander; Fiscal Year: 1980
    ..Other experiments, involving NMR and flash-photolysis techniques, will be done to study kinetic and equilibrium aspects of O2-penetration into heme and non-heme proteins...
  53. CARDIOVASCULAR RESPONSE TO GLUTAMATE NITRIC OXIDE
    WILLIAM TALMAN; Fiscal Year: 1999
    ....
  54. CARDIOVASCULAR RESPONSE TO GLUTAMATE NITRIC OXIDE
    WILLIAM TALMAN; Fiscal Year: 2001
    ....
  55. CARDIOVASCULAR RESPONSE TO GLUTAMATE NITRIC OXIDE
    WILLIAM TALMAN; Fiscal Year: 2000
    ....
  56. INVESTIGATIONS OF HEMOGLOBIN S PROPERTIES
    ELIZABETH SIMONS; Fiscal Year: 1980
    ..h) the preparation and study of reconstituted ghosts which contain, instead of hemoglobin, a protein capable of gelling and of deforming the cell into a classical sickled shape...
  57. CARDIOVASCULAR RESPONSE TO GLUTAMATE NITRIC OXIDE
    WILLIAM TALMAN; Fiscal Year: 2002
    ....
  58. Treatment of Hyperglycemia Ischemic Stroke(THIS)Trial
    Askiel Bruno; Fiscal Year: 2003
    ..Additional measurements will include vital signs, serum electrolytes, hemoglobin A-1C, and a global test for multiple clinical outcomes at 3 months.
  59. Treatment of Hyperglycemia Ischemic Stroke(THIS)Trial
    Askiel Bruno; Fiscal Year: 2004
    ..Additional measurements will include vital signs, serum electrolytes, hemoglobin A-1C, and a global test for multiple clinical outcomes at 3 months.
  60. Treatment of Hyperglycemia Ischemic Stroke(THIS)Trial
    Askiel Bruno; Fiscal Year: 2002
    ..Additional measurements will include vital signs, serum electrolytes, hemoglobin A-1C, and a global test for multiple clinical outcomes at 3 months.
  61. STRUCTURAL BASIS FOR ASSEMBLY OF COOPERATIVE HEMOGLOBINS
    WILLIAM ROYER; Fiscal Year: 1999
    ....
  62. STRUCTURAL BASIS FOR ASSEMBLY OF COOPERATIVE HEMOGLOBINS
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