fibrin

Summary

Summary: A protein derived from FIBRINOGEN in the presence of THROMBIN, which forms part of the blood clot.

Top Publications

  1. ncbi Fibrinogen and fibrin structure and functions
    M W Mosesson
    The Blood Research Institute, The Blood Center of Southeastern Wisconsin, Milwaukee, WI 53201 2178, USA
    J Thromb Haemost 3:1894-904. 2005
  2. pmc Microporous nanofibrous fibrin-based scaffolds for bone tissue engineering
    Thanaphum Osathanon
    Department of Oral Biology, School of Dentistry, University of Washington, Seattle, WA 98195, United States
    Biomaterials 29:4091-9. 2008
  3. ncbi Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part II: platelet-related biologic features
    David M Dohan
    Biophysics Laboratory, Faculty of Dental Surgery, University of Paris V, Paris, France
    Oral Surg Oral Med Oral Pathol Oral Radiol Endod 101:e45-50. 2006
  4. doi Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leucocyte- and platelet-rich fibrin (L-PRF)
    David M Dohan Ehrenfest
    Department of Biomaterials, Institute of Clinical Sciences, the Sahlgrenska Academy at University of Gothenburg, Sweden
    Trends Biotechnol 27:158-67. 2009
  5. pmc Annexin II regulates fibrin homeostasis and neoangiogenesis in vivo
    Qi Ling
    Department of Cell and Developmental Biology, Weill Medical College of Cornell University, New York, New York 10021, USA
    J Clin Invest 113:38-48. 2004
  6. doi Interaction of fibrin with red blood cells: the role of iron
    Boguslaw Lipinski
    Harvard Medical School, Boston, Massachusetts, USA
    Ultrastruct Pathol 36:79-84. 2012
  7. ncbi Fibrin as a cell carrier in cardiovascular tissue engineering applications
    Anita Mol
    Department of Biomedical Engineering, Eindhoven University of Technology, PO Box 513, 5600 MB Eindhoven, The Netherlands
    Biomaterials 26:3113-21. 2005
  8. ncbi Engineering of fibrin-based functional and implantable small-diameter blood vessels
    Daniel D Swartz
    Department of Physiology and Biophysics, State University of New York, Buffalo, New York 14260, USA
    Am J Physiol Heart Circ Physiol 288:H1451-60. 2005
  9. ncbi A proposed reference method for plasminogen activators that enables calculation of enzyme activities in SI units
    C Longstaff
    Division of Haematology, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, UK
    J Thromb Haemost 2:1416-21. 2004
  10. pmc Novel aspects of fibrin(ogen) fragments during inflammation
    Carla Jennewein
    Clinic of Anesthesiology, Intensive Care Medicine and Pain Therapy, Goethe University Hospital Frankfurt, Frankfurt am Main, Germany
    Mol Med 17:568-73. 2011

Detail Information

Publications379 found, 100 shown here

  1. ncbi Fibrinogen and fibrin structure and functions
    M W Mosesson
    The Blood Research Institute, The Blood Center of Southeastern Wisconsin, Milwaukee, WI 53201 2178, USA
    J Thromb Haemost 3:1894-904. 2005
    ..Each molecule contains two outer D domains connected to a central E domain by a coiled-coil segment. Fibrin is formed after thrombin cleavage of fibrinopeptide A (FPA) from fibrinogen Aalpha-chains, thus initiating fibrin ..
  2. pmc Microporous nanofibrous fibrin-based scaffolds for bone tissue engineering
    Thanaphum Osathanon
    Department of Oral Biology, School of Dentistry, University of Washington, Seattle, WA 98195, United States
    Biomaterials 29:4091-9. 2008
    ..Microporous, nanofibrous fibrin scaffolds were fabricated using sphere-templating methods...
  3. ncbi Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part II: platelet-related biologic features
    David M Dohan
    Biophysics Laboratory, Faculty of Dental Surgery, University of Paris V, Paris, France
    Oral Surg Oral Med Oral Pathol Oral Radiol Endod 101:e45-50. 2006
    Platelet-rich fibrin (PRF) belongs to a new generation of platelet concentrates, with simplified processing and without biochemical blood handling...
  4. doi Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leucocyte- and platelet-rich fibrin (L-PRF)
    David M Dohan Ehrenfest
    Department of Biomaterials, Institute of Clinical Sciences, the Sahlgrenska Academy at University of Gothenburg, Sweden
    Trends Biotechnol 27:158-67. 2009
    ..classification of the different platelet concentrates into four categories, depending on their leucocyte and fibrin content: pure platelet-rich plasma (P-PRP), such as cell separator PRP, Vivostat PRF or Anitua's PRGF; leucocyte- ..
  5. pmc Annexin II regulates fibrin homeostasis and neoangiogenesis in vivo
    Qi Ling
    Department of Cell and Developmental Biology, Weill Medical College of Cornell University, New York, New York 10021, USA
    J Clin Invest 113:38-48. 2004
    ..t-PA- dependent) conversion of plasminogen to active plasmin requires the presence of the cofactor/substrate fibrin. However, previous in vitro studies have suggested that the endothelial cell surface protein annexin II can ..
  6. doi Interaction of fibrin with red blood cells: the role of iron
    Boguslaw Lipinski
    Harvard Medical School, Boston, Massachusetts, USA
    Ultrastruct Pathol 36:79-84. 2012
    Activation of coagulation pathways results in the formation of hemostatic fibrin plugs...
  7. ncbi Fibrin as a cell carrier in cardiovascular tissue engineering applications
    Anita Mol
    Department of Biomedical Engineering, Eindhoven University of Technology, PO Box 513, 5600 MB Eindhoven, The Netherlands
    Biomaterials 26:3113-21. 2005
    ..In this study, seeding by using fibrin as a cell carrier is compared to the conventional static seeding method with regard to tissue development...
  8. ncbi Engineering of fibrin-based functional and implantable small-diameter blood vessels
    Daniel D Swartz
    Department of Physiology and Biophysics, State University of New York, Buffalo, New York 14260, USA
    Am J Physiol Heart Circ Physiol 288:H1451-60. 2005
    ..engineered implantable small-diameter blood vessels based on ovine smooth muscle and endothelial cells embedded in fibrin gels...
  9. ncbi A proposed reference method for plasminogen activators that enables calculation of enzyme activities in SI units
    C Longstaff
    Division of Haematology, National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, UK
    J Thromb Haemost 2:1416-21. 2004
    ..Plasminogen activation is monitored in a transparent, solid fibrin matrix but uses chromogenic substrate hydrolysis, rather than changes in fibrin, to quantitate the activity of PAs...
  10. pmc Novel aspects of fibrin(ogen) fragments during inflammation
    Carla Jennewein
    Clinic of Anesthesiology, Intensive Care Medicine and Pain Therapy, Goethe University Hospital Frankfurt, Frankfurt am Main, Germany
    Mol Med 17:568-73. 2011
    ..Coagulation is initiated by the activation of thrombin, which, in turn, triggers fibrin formation by the release of fibrinopeptides...
  11. ncbi Molecular mechanisms involved in the resistance of fibrin to clot lysis by plasmin in subjects with type 2 diabetes mellitus
    E J Dunn
    Academic Unit of Molecular Vascular Medicine, Leeds Institute for Genetics, Health and Therapeutics, University of Leeds, Clarendon Way, Leeds LS1 3EX, UK
    Diabetologia 49:1071-80. 2006
    ..the influence of type 2 diabetes on fibrinolysis by assessing interactions between the regulatory components of fibrinolysis and the fibrin clot, using fibrinogen purified from 150 patients with type 2 diabetes and 50 matched controls.
  12. doi Surface modification with fibrin/hyaluronic acid hydrogel on solid-free form-based scaffolds followed by BMP-2 loading to enhance bone regeneration
    Sun Woong Kang
    Department of Biomedical Science, CHA University, Seoul, Republic of Korea
    Bone 48:298-306. 2011
    ..In the present study, the surface of a scaffold fabricated using MHDS was coated with a mixture of fibrin and hyaluronic acid (HA) and used as a vehicle for delivery of both bone morphogenetic protein-2 (BMP-2) and ..
  13. pmc Activation of mannan-binding lectin-associated serine proteases leads to generation of a fibrin clot
    Krishana C Gulla
    School of Life Sciences, DA University, Indore, India
    Immunology 129:482-95. 2010
    ..Plasmin digestion of the clot and analysis using anti-D-dimer antibodies revealed that the clot was made up of fibrin and was similar to that generated by thrombin in normal human plasma...
  14. ncbi Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part I: technological concepts and evolution
    David M Dohan
    Biophysics Laboratory, Faculty of Dental Surgery, University of Paris V, Paris, France
    Oral Surg Oral Med Oral Pathol Oral Radiol Endod 101:e37-44. 2006
    Platelet-rich fibrin (PRF) belongs to a new generation of platelet concentrates geared to simplified preparation without biochemical blood handling...
  15. pmc Protective roles for fibrin, tissue factor, plasminogen activator inhibitor-1, and thrombin activatable fibrinolysis inhibitor, but not factor XI, during defense against the gram-negative bacterium Yersinia enterocolitica
    Deyan Luo
    Trudeau Institute, Saranac Lake, NY 12983, USA
    J Immunol 187:1866-76. 2011
    ..work may help to explain this discrepancy by demonstrating critical protective roles for coagulation leading to fibrin deposition during host defense against the Gram-negative bacterium Yersinia enterocolitica. After i.p...
  16. ncbi Platelet-rich fibrin: evolution of a second-generation platelet concentrate
    V Sunitha Raja
    Department of Periodontics, Meenakshiammal Dental College and Hospital, Maduravoyal, Chennai, India
    Indian J Dent Res 19:42-6. 2008
    ..The preparation of PRP has been described by several authors. Platelet-rich fibrin (PRF) was first described by Choukroun et al. in France...
  17. ncbi Fibroblast invasive migration into fibronectin/fibrin gels requires a previously uncharacterized dermatan sulfate-CD44 proteoglycan
    Richard A F Clark
    Department of Dermatology, School of Medicine, SUNY at Stony Brook, New York, 11794 8165, USA
    J Invest Dermatol 122:266-77. 2004
    After tissue injury, fibroblast migration from the peri-wound collagenous stroma into the fibrin-laden wound is critical for granulation tissue formation and subsequent healing...
  18. pmc Type I collagen, fibrin and PuraMatrix matrices provide permissive environments for human endothelial and mesenchymal progenitor cells to form neovascular networks
    Patrick Allen
    Vascular Biology Program, Children s Hospital, Boston, MA 02115, USA
    J Tissue Eng Regen Med 5:e74-86. 2011
    ..cells (EPCs) and mesenchymal progenitor cells (MPCs) could form microvascular networks in type I collagen, fibrin and an engineered peptide hydrogel, PuraMatrix, in 7 days in vivo in immune-deficient mice...
  19. pmc Sequential binding of αVβ3 and ICAM-1 determines fibrin-mediated melanoma capture and stable adhesion to CD11b/CD18 on neutrophils
    Pu Zhang
    Department of Bioengineering, The Pennsylvania State University, University Park, PA 16802, USA
    J Immunol 186:242-54. 2011
    b>Fibrin (Fn) deposition defines several type 1 immune responses, including delayed-type hypersensitivity and autoimmunity in which polymorphonuclear leukocytes (PMNs) are involved...
  20. doi Fibrinogen, fibrin, and FDP induce C-reactive protein generation in rat vascular smooth muscle cells: pro-inflammatory effect on atherosclerosis
    Fang Guo
    Department of Pharmacology, Xi an Jiaotong University School of Medicine, People s Republic of China
    Biochem Biophys Res Commun 390:942-6. 2009
    ..The purpose of the present study was to observe the effect of fibrinogen, fibrin, and fibrin degradation products (FDP) on CRP generation in VSMCs...
  21. pmc A fibrin patch-based enhanced delivery of human embryonic stem cell-derived vascular cell transplantation in a porcine model of postinfarction left ventricular remodeling
    Qiang Xiong
    Department of Medicine, University of Minnesota Medical School, Minneapolis, Minnesota 5545, USA
    Stem Cells 29:367-75. 2011
    ..transplantation of hESC-derived endothelial cells (ECs) and hESC-derived smooth muscle cells (SMCs) with a fibrin three-dimensional (3D) porous scaffold biomatrix...
  22. ncbi Mass + retention time = structure: a strategy for the analysis of N-glycans by carbon LC-ESI-MS and its application to fibrin N-glycans
    Martin Pabst
    Biochemistry Division, Department of Chemistry, University of Natural Resources and Applied Life Sciences BOKU, Muthgasse 18, 1190 Vienna, Austria
    Anal Chem 79:5051-7. 2007
    ..The method was applied for the detailed structural analysis of fibrin(ogen) N-glycans from various species (human, cow, pig, mouse, rat, cat, dog, Chinese hamster, horse, sheep, and ..
  23. ncbi Fibrin structure and wound healing
    N Laurens
    Department of Biomedical Research, TNO Quality of Life, Gaubius Laboratory, Leiden, The Netherlands
    J Thromb Haemost 4:932-9. 2006
    Fibrinogen and fibrin play an important role in blood clotting, fibrinolysis, cellular and matrix interactions, inflammation, wound healing, angiogenesis, and neoplasia...
  24. ncbi Fibrin and fibrinolysis in infection and host defense
    J L Degen
    Division of Developmental Biology, Children s Hospital Research Foundation and the University of Cincinnati College of Medicine, Cincinnati, OH, USA
    J Thromb Haemost 5:24-31. 2007
    ..and other hemostatic factors have begun to reveal a seminal role of bacterial plasminogen activators and fibrin clearance in microbial pathogenesis...
  25. ncbi Fibrinogen and fibrin
    John W Weisel
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6058, USA
    Adv Protein Chem 70:247-99. 2005
    ..which are in the central region, are cleaved by thrombin to convert soluble fibrinogen to insoluble fibrin polymer, via intermolecular interactions of the "knobs" exposed by fibrinopeptide removal with "..
  26. ncbi The structure and biological features of fibrinogen and fibrin
    M W Mosesson
    Blood Research Institute, Blood Center of Southeastern Wisconsin, P O Box 2178, Milwaukee, Wisconsin 53201, USA
    Ann N Y Acad Sci 936:11-30. 2001
    Fibrinogen and fibrin play important, overlapping roles in blood clotting, fibrinolysis, cellular and matrix interactions, inflammation, wound healing, and neoplasia...
  27. ncbi Fibrin glue alone and skeletal myoblasts in a fibrin scaffold preserve cardiac function after myocardial infarction
    Karen L Christman
    University of California Berkeley and San Francisco Joint Bioengineering Graduate Group, San Francisco, California, USA
    Tissue Eng 10:403-9. 2004
    ..In this study, we examined the effects of fibrin glue as an injectable scaffold and wall support in ischemic myocardium...
  28. ncbi Angiogenic sprouting and capillary lumen formation modeled by human umbilical vein endothelial cells (HUVEC) in fibrin gels: the role of fibroblasts and Angiopoietin-1
    Martin N Nakatsu
    Department of Molecular Biology and Biochemistry, 3219 McGaugh Hall, University of California, Irvine, Irvine, CA 92697, USA
    Microvasc Res 66:102-12. 2003
    ..report an optimized protocol for human umbilical vein EC in which EC sprout from the surface of beads embedded in fibrin gels...
  29. ncbi Platelets and fibrin(ogen) increase metastatic potential by impeding natural killer cell-mediated elimination of tumor cells
    Joseph S Palumbo
    Division of Hematology Oncology, Children s Hospital Research Foundation, and University of Cincinnati College of Medicine, Cincinnati, OH, USA
    Blood 105:178-85. 2005
    ..an important link between hemostatic factors and innate immunity and indicate that one mechanism by which the platelet-fibrin(ogen) axis contributes to metastatic potential is by impeding natural killer cell elimination of tumor cells.
  30. pmc Selective abrogation of the uPA-uPAR interaction in vivo reveals a novel role in suppression of fibrin-associated inflammation
    Brian M Connolly
    Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA
    Blood 116:1593-603. 2010
    ..mice revealed an unanticipated role of the uPA-uPAR interaction in suppressing inflammation secondary to fibrin deposition...
  31. pmc Functional production and characterization of a fibrin-specific single-chain antibody fragment from Bacillus subtilis: effects of molecular chaperones and a wall-bound protease on antibody fragment production
    Sau Ching Wu
    Division of Cellular, Molecular and Microbial Biology, Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, Alberta T2N 1N4, Canada
    Appl Environ Microbiol 68:3261-9. 2002
    To develop an ideal blood clot imaging and targeting agent, a single-chain antibody (SCA) fragment based on a fibrin-specific monoclonal antibody, MH-1, was constructed and produced via secretion from Bacillus subtilis...
  32. doi Enhancing efficacy of stem cell transplantation to the heart with a PEGylated fibrin biomatrix
    Ge Zhang
    Department of Biomedical Engineering, University of Texas, Austin, Texas 78712 0238, USA
    Tissue Eng Part A 14:1025-36. 2008
    ..We investigated whether delivery of BMNCs by an injectable PEGylated fibrin biomatrix that covalently binds hepatocyte growth factor (HGF) would enhance the rate of cell engraftment and ..
  33. ncbi Structure of fibrin: impact on clot stability
    J W Weisel
    Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6058, USA
    J Thromb Haemost 5:116-24. 2007
    ..on the structural origins of clot stability is necessary for understanding the functions and pathology of fibrin clots and thrombi, but is also important for interpreting correctly the results of a variety of clinical ..
  34. doi Factor XIII: a coagulation factor with multiple plasmatic and cellular functions
    Laszlo Muszbek
    Clinical Research Center and Thrombosis, Haemostasis and Vascular Biology Research Group of the Hungarian Academy of Sciences, University of Debrecen, Medical and Health Science Center, Debrecen, Hungary
    Physiol Rev 91:931-72. 2011
    ..New aspects of the traditional functions of FXIII, stabilization of fibrin clot, and protection of fibrin against fibrinolysis are summarized...
  35. pmc Talin-dependent integrin activation is required for fibrin clot retraction by platelets
    Jacob R Haling
    Department of Medicine, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
    Blood 117:1719-22. 2011
    ..deletion of talin, we show for the first time that the capacity of talin to activate integrins is required for fibrin clot retraction by platelets...
  36. doi Novel composite hyaluronan/type I collagen/fibrin scaffold enhances repair of osteochondral defect in rabbit knee
    Eva Filova
    Department of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    J Biomed Mater Res B Appl Biomater 87:415-24. 2008
    A new composite scaffold containing type I collagen, hyaluronan, and fibrin was prepared with and without autologous chondrocytes and implanted into a rabbit femoral trochlea...
  37. pmc Fibrin(ogen) mediates acute inflammatory responses to biomaterials
    L Tang
    Division of Experimental Pathology, Albany Medical College, New York 12208
    J Exp Med 178:2147-56. 1993
    ..Thus, spontaneous adsorption of fibrinogen appears to initiate the acute inflammatory response to an implanted polymer, suggesting an interesting nexus between two major iatrogenic effects of biomaterials: clotting and inflammation...
  38. pmc Fibrin gel-immobilized VEGF and bFGF efficiently stimulate angiogenesis in the AV loop model
    Andreas Arkudas
    Department of Plastic and Hand Surgery, University of Erlangen Medical Center, Erlangen, Germany
    Mol Med 13:480-7. 2007
    ..We assessed the angiogenic effects of fibrin-immobilized VEGF and bFGF in an arteriovenous loop (AVL) model in 22 AVLs created between the femoral artery and ..
  39. pmc Sildenafil attenuates pulmonary inflammation and fibrin deposition, mortality and right ventricular hypertrophy in neonatal hyperoxic lung injury
    Yvonne P de Visser
    Department of Pediatrics, Division of Neonatology, Leiden University Medical Center, 2300 RC Leiden, The Netherlands
    Respir Res 10:30. 2009
    ....
  40. pmc Nanostructure of the fibrin clot
    C Yeromonahos
    Centre National de la Recherche Scientifique, Universite Joseph Fourier, Grenoble, France
    Biophys J 99:2018-27. 2010
    The nanostructure of the fibrin fibers in fibrin clots is investigated by using spectrometry and small angle x-ray scattering measurements...
  41. pmc Role of the Enterococcus faecalis GelE protease in determination of cellular chain length, supernatant pheromone levels, and degradation of fibrin and misfolded surface proteins
    Christopher M Waters
    Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    J Bacteriol 185:3613-23. 2003
    ..GelE was also essential for degradation of polymerized fibrin. GelE expression reduced the titer of cCF10, the peptide pheromone that induces conjugation of pCF10, and pCF10 ..
  42. pmc Polyphosphate enhances fibrin clot structure
    Stephanie A Smith
    Departments of Internal Medicine and Biochemistry, College of Medicine, University of Illinois at Urbana Champaign, USA
    Blood 112:2810-6. 2008
    ..We now report that polyphosphate enhances fibrin clot structure in a calcium-dependent manner...
  43. pmc The development of bleomycin-induced pulmonary fibrosis in mice deficient for components of the fibrinolytic system
    C M Swaisgood
    Joseph J Jacobs Center for Thrombosis and Vascular Biology and the Department of Molecular Cardiology, Cleveland Clinic Foundation, OH, USA
    Am J Pathol 157:177-87. 2000
    ..Absence of these two components of the fibrinolytic system, either urokinase or plasminogen, results in accelerated fibrosis...
  44. pmc Thrombin flux and wall shear rate regulate fibrin fiber deposition state during polymerization under flow
    K B Neeves
    Department of Chemical Engineering, Colorado School of Mines, Golden, Colorado, USA
    Biophys J 98:1344-52. 2010
    Thrombin is released as a soluble enzyme from the surface of platelets and tissue-factor-bearing cells to trigger fibrin polymerization during thrombosis under flow conditions...
  45. ncbi Properties of engineered vascular constructs made from collagen, fibrin, and collagen-fibrin mixtures
    Christopher L Cummings
    Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, USA
    Biomaterials 25:3699-706. 2004
    ..were formed by embedding rat aortic smooth muscle cells in three-dimensional matrices of Type I collagen, fibrin, or a mixture of collagen and fibrin in a 1:1 ratio, at total matrix protein concentrations of 2 and 4 mg/ml...
  46. pmc Matrix composition regulates three-dimensional network formation by endothelial cells and mesenchymal stem cells in collagen/fibrin materials
    Rameshwar R Rao
    Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA
    Angiogenesis 15:253-64. 2012
    ..In this study, vessel-like network formation was studied using 3D collagen/fibrin (COL/FIB) matrices seeded with embedded EC and MSC and cultured for 7 days...
  47. doi The optimal carrier for BMP-2: a comparison of collagen versus fibrin matrix
    Sebastian Schützenberger
    Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Centre, Austrian Cluster for Tissue Regeneration, Donaueschingenstrasse 13, 1200, Vienna, Austria
    Arch Orthop Trauma Surg 132:1363-70. 2012
    ..in vitro and in a new in vivo rat model for impaired bone healing whether a low dose BMP-2 preparation in fibrin would be equivalent or better than the combination of collagen and a high dose of BMP-2 which is currently in ..
  48. doi Osteotome sinus floor elevation using Choukroun's platelet-rich fibrin as grafting material: a 1-year prospective pilot study with microthreaded implants
    Antoine Diss
    LASIO, Odontology Faculty, Nice University, France
    Oral Surg Oral Med Oral Pathol Oral Radiol Endod 105:572-9. 2008
    ..changes in the apical bone levels on microthreaded implants placed in subsinus residual bone height, according to a bone-added osteotome sinus floor elevation technique with platelet-rich fibrin (PRF) as grafting material.
  49. ncbi Isolation of mesenchymal stem cells from G-CSF-mobilized human peripheral blood using fibrin microbeads
    I Kassis
    Radiobiology and Biotechnology Laboratory, Sharett Institute of Oncology, Hadassah Hebrew University Medical Center, Jerusalem, Israel
    Bone Marrow Transplant 37:967-76. 2006
    ..b>Fibrin microbeads (FMB) that bind matrix-dependent cells were used to isolate MSC from the mononuclear fraction of ..
  50. doi Inflammation and coagulation. An overview
    Jari Petaja
    Director, Department of Pediatrics and Gynecology Helsinki University Central Hospital, Finland
    Thromb Res 127:S34-7. 2011
    ..Animal experiments with signaling selective APC-variants show promise in improving the therapeutic efficacy and safety of APC in sepsis...
  51. doi Effects of cigarette smoke exposure on clot dynamics and fibrin structure: an ex vivo investigation
    Rajat S Barua
    Division of Cardiovascular Medicine, University of California San Francisco, Fresno, 2823 N Fresno Street, Fresno, CA 93721, USA
    Arterioscler Thromb Vasc Biol 30:75-9. 2010
    The purpose of this study was to examine the effect of cigarette smoke exposure (CSE) on clot dynamics and fibrin architecture and to isolate the relative contribution of platelets and fibrinogen to clot dynamics.
  52. doi Controlled preparation of thin fibrin films immobilized at solid surfaces
    Tomas Riedel
    Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    J Biomed Mater Res A 88:437-47. 2009
    A technique for coating surfaces with attached fibrin structures without the formation of fibrin gel in bulk solution was developed...
  53. ncbi Pathological analysis of local delivery of paclitaxel via a polymer-coated stent
    A Farb
    Department of Cardiovascular Pathology, Armed Forces Institute of Pathology, Washington, DC, USA
    Circulation 104:473-9. 2001
    ..0 and 20.2 microgram) paclitaxel-containing stents consisting of persistent intimal fibrin deposition, intraintimal hemorrhage, and increased intimal and adventitial inflammation...
  54. pmc Thrombin generation, fibrin clot formation and hemostasis
    Alisa S Wolberg
    Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, 815 Brinkhous Bullitt Building, CB 7525, Chapel Hill, NC 27599 7525, USA
    Transfus Apher Sci 38:15-23. 2008
    Hemostatic clot formation entails thrombin-mediated cleavage of fibrinogen to fibrin. Previous in vitro studies have shown that the thrombin concentration present during clot formation dictates the ultimate fibrin structure...
  55. pmc Bone marrow stromal cells stimulate an angiogenic program that requires endothelial MT1-MMP
    Suraj Kachgal
    Department of Biomedical Engineering, University of California, Irvine, Irvine, CA, USA
    J Cell Physiol 227:3546-55. 2012
    ..In addition to preventing fibrosis at the wound site, BMSCs elicit an angiogenic response within the fibrin matrix...
  56. pmc Concentration independent modulation of local micromechanics in a fibrin gel
    Maxwell A Kotlarchyk
    Department of Biomedical Engineering, University of California Irvine, Irvine, California, United States of America
    PLoS ONE 6:e20201. 2011
    ..Here we present the device in the context of fibrin hydrogels...
  57. ncbi Enhancement of endogenous fibrinolysis does not reduce local fibrin deposition, but modulates inflammation upon intestinal ischemia and reperfusion
    Ivo G Schoots
    Department of Surgery, Academic Medical Center, University of Amsterdam, The Netherlands
    Thromb Haemost 91:497-505. 2004
    This study investigated the contribution of endogenous suppression of fibrinolysis and increased fibrin deposition to intestinal dysfunction and injury in a rat model of intestinal ischemia/reperfusion (I/R), as fibrinolytic inhibition ..
  58. pmc Differential contributions of monocyte- and platelet-derived microparticles towards thrombin generation and fibrin formation and stability
    M M Aleman
    Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, NC 27599 7525, USA
    J Thromb Haemost 9:2251-61. 2011
    ..Microparticles (MPs) are sub-micron vesicles shed by activated or apoptotic cells, including platelets and monocytes. Increased circulating MPs are associated with thrombosis; however, their role in thrombogenesis is poorly understood...
  59. doi Reduced thrombin formation and altered fibrin clot properties induced by polyunsaturated omega-3 fatty acids on top of dual antiplatelet therapy in patients undergoing percutaneous coronary intervention (OMEGA-PCI clot)
    Grzegorz Gajos
    Department of Coronary Disease, Institute of Cardiology, Jagiellonian University Medical College, Krakow, Poland
    Arterioscler Thromb Vasc Biol 31:1696-702. 2011
    ..of this study was to investigate whether omega-3 polyunsaturated fatty acids (n-3 PUFA) are able to alter plasma fibrin clot properties and reduce thrombin formation in stable coronary artery disease patients undergoing percutaneous ..
  60. ncbi Trial with Platelet-Rich Fibrin and Bio-Oss used as grafting materials in the treatment of the severe maxillar bone atrophy: clinical and radiological evaluations
    F Inchingolo
    School in Dental Hygiene, Faculty of Medicine, University Aldo Moro, Bari, Italy
    Eur Rev Med Pharmacol Sci 14:1075-84. 2010
    ....
  61. ncbi Enzymatic formation of modular cell-instructive fibrin analogs for tissue engineering
    Martin Ehrbar
    Oral Biology, Section Bioengineering, Department of Cranio Maxillofacial Surgery, University Hospital Zurich, Zurich, Switzerland
    Biomaterials 28:3856-66. 2007
    ..biomaterials by recapitulating the crosslinking chemistry and the biomolecular characteristics of the biopolymer fibrin in a synthetic analog...
  62. pmc Urokinase-type plasminogen activator is effective in fibrin clearance in the absence of its receptor or tissue-type plasminogen activator
    T H Bugge
    Division of Developmental Biology, Children s Hospital Research Foundation, Cincinnati, OH 45229, USA
    Proc Natl Acad Sci U S A 93:5899-904. 1996
    ..Sinusoidal fibrin deposits are found within the livers of nearly all adult mice examined with a dual deficiency in uPAR and tPA, ..
  63. ncbi Endothelial tubulogenesis within fibrin gels specifically requires the activity of membrane-type-matrix metalloproteinases (MT-MMPs)
    Marc A Lafleur
    School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, UK
    J Cell Sci 115:3427-38. 2002
    Macro- and microvascular endothelial cells (EC) formed tubular structures when cultured within a 3D fibrin matrix, a process that was enhanced by vascular endothelial growth factor (VEGF), fibroblast growth factor-2 (FGF-2), hepatocyte ..
  64. ncbi Role of factor XIII in fibrin clot formation and effects of genetic polymorphisms
    Robert A S Ariens
    Academic Unit of Molecular Vascular Medicine, University of Leeds School of Medicine, United Kingdom
    Blood 100:743-54. 2002
    Factor XIII and fibrinogen are unusual among clotting factors in that neither is a serine protease. Fibrin is the main protein constituent of the blood clot, which is stabilized by factor XIIIa through an amide or isopeptide bond that ..
  65. doi Secretion of fibrinolytic enzymes facilitates human mesenchymal stem cell invasion into fibrin clots
    Sabine Neuss
    Institute of Pathology, BioInterface Group, RWTH Aachen University, Aachen, Germany
    Cells Tissues Organs 191:36-46. 2010
    ..b>Fibrin clot formation is associated with most wound healing processes and potentially guides the recruitment of hMSC...
  66. pmc Simultaneous activation of complement and coagulation by MBL-associated serine protease 2
    Anders Krarup
    MRC Immunochemistry Unit, Department of Biochemistry, University of Oxford, Oxford, United Kingdom
    PLoS ONE 2:e623. 2007
    ..shown that the thrombin generated is active and can cleave both factor XIII and fibrinogen, forming cross-linked fibrin. To explore the biological significance of these findings we showed that fibrin was covalently bound on a ..
  67. doi Molecular MRI of early thrombus formation using a bimodal alpha2-antiplasmin-based contrast agent
    Robbert Jan J H M Miserus
    Department of Radiology, Maastricht University Medical Centre, Maastricht, The Netherlands
    JACC Cardiovasc Imaging 2:987-96. 2009
    ..We aimed to investigate whether early thrombus formation can be visualized with in vivo magnetic resonance imaging (MRI) by the use of a novel bimodal alpha(2)-antiplasmin-based contrast agent (CA)...
  68. ncbi Autologous stem cell transplantation for myocardial repair
    Jingbo Liu
    Department of Medicine, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    Am J Physiol Heart Circ Physiol 287:H501-11. 2004
    ..The patch consisted of a fibrin matrix seeded with autologous porcine mesenchymal stem cells labeled with lacZ...
  69. doi A composite fibrin-based scaffold for controlled delivery of bioactive pro-angiogenetic growth factors
    Enrica Briganti
    Laboratory for Biomaterials and Graft Technology, G Pasquinucci Hospital, Institute of Clinical Physiology, CNR, 54100Massa, Italy
    J Control Release 142:14-21. 2010
    ..synthetic biocompatible material, a poly(ether)urethane-polydimethylsiloxane blend, and a biological polymer, the fibrin, were manufactured by spray, phase-inversion technique...
  70. pmc Fibrin-based tissue engineering scaffolds enhance neural fiber sprouting and delay the accumulation of reactive astrocytes at the lesion in a subacute model of spinal cord injury
    Philip J Johnson
    Department of Biomedical Engineering, Washington University, St Louis, MO 63130, USA
    J Biomed Mater Res A 92:152-63. 2010
    The purpose of this study was to evaluate the effects of fibrin scaffolds on subacute rat spinal cord injury (SCI)...
  71. ncbi Use of soluble fibrin antigen instead of D-dimer as fibrin-related marker may enhance the prognostic power of the ISTH overt DIC score
    Carl Erik Dempfle
    University Hospital of Mannheim, I Department of Medicine, Theodor Kutzer Ufer 1 3, D 68167 Mannheim, Germany
    Thromb Haemost 91:812-8. 2004
    The overt DIC score of the DIC subcommittee of the ISTH includes a fibrin-related marker (FRM) as indicator of intravascular fibrin formation...
  72. pmc Multiscale mechanics of fibrin polymer: gel stretching with protein unfolding and loss of water
    Andre E X Brown
    Department of Physics and Astronomy, University of Pennsylvania, Philadelphia, PA 19104, USA
    Science 325:741-4. 2009
    Blood clots and thrombi consist primarily of a mesh of branched fibers made of the protein fibrin. We propose a molecular basis for the marked extensibility and negative compressibility of fibrin gels based on the structural and ..
  73. pmc The effect of matrix density on the regulation of 3-D capillary morphogenesis
    Cyrus M Ghajar
    Department of Biomedical Engineering, University of California, Irvine, Irvine, California 92697, USA
    Biophys J 94:1930-41. 2008
    ..To study this phenomenon, we utilized a fibrin-based in vitro assay in which a fibroblast monolayer is plated atop a fibrin gel approximately 2...
  74. pmc Direct comparisons of the morphology, migration, cell adhesions, and actin cytoskeleton of fibroblasts in four different three-dimensional extracellular matrices
    Kirsi M Hakkinen
    Laboratory of Cell and Developmental Biology, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892 4370, USA
    Tissue Eng Part A 17:713-24. 2011
    ..cell adhesion and migration by human foreskin fibroblasts in cell-derived matrix or hydrogels of collagen type I, fibrin, or basement membrane extract (BME)...
  75. pmc Soft fibrin gels promote selection and growth of tumorigenic cells
    Jing Liu
    Department of Biochemistry and Molecular Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China
    Nat Mater 11:734-41. 2012
    ..We developed a mechanical method for selecting tumorigenic cells by culturing single cancer cells in fibrin matrices of ~100 Pa in stiffness...
  76. pmc Endothelial progenitor cells are integrated in newly formed capillaries and alter adjacent fibrovascular tissue after subcutaneous implantation in a fibrin matrix
    Oliver Bleiziffer
    Department of Plastic and Hand Surgery, University Hospital Erlangen, Erlangen, Germany
    J Cell Mol Med 15:2452-61. 2011
    ..The transplanted cells were histologically detected at all time-points and located almost exclusively within the fibrin matrix at day 3 but the number of cells in the clot continuously decreased over day 7 to day 14...
  77. pmc Leukocyte engagement of fibrin(ogen) via the integrin receptor alphaMbeta2/Mac-1 is critical for host inflammatory response in vivo
    Matthew J Flick
    Children s Hospital Research Foundation and the University of Cincinnati College of Medicine, Cincinnati, Ohio 45229 3039, USA
    J Clin Invest 113:1596-606. 2004
    ..appears to support the inflammatory response through multiple ligands, but local engagement of fibrin(ogen) may be particularly important for leukocyte function...
  78. doi Fibrin clot structure and function: a role in the pathophysiology of arterial and venous thromboembolic diseases
    Anetta Undas
    Institute of Cardiology, Jagiellonian University School of Medicine, Krakow, Poland
    Arterioscler Thromb Vasc Biol 31:e88-99. 2011
    The formation of fibrin clots that are relatively resistant to lysis represents the final step in blood coagulation. We discuss the genetic and environmental regulators of fibrin structure in relation to thrombotic disease...
  79. pmc Fibrin deposition accelerates neurovascular damage and neuroinflammation in mouse models of Alzheimer's disease
    Justin Paul
    Laboratory of Neurobiology and Genetics, The Rockefeller University, New York, NY 10065, USA
    J Exp Med 204:1999-2008. 2007
    ..mouse models of AD (TgCRND8, PDAPP, and Tg2576), we evaluated blood-brain barrier damage and the role of fibrin and fibrinolysis in the progression of amyloid-beta pathology...
  80. ncbi Dysferlin is a new marker for leaky brain blood vessels in multiple sclerosis
    Sonja Hochmeister
    Division of Neuroimmunology, Center for Brain Research, Medical University of Vienna, Vienna, Austria
    J Neuropathol Exp Neurol 65:855-65. 2006
    ..Hence, dysferlin is not only a marker for leaky brain vessels, but also reveals dissociation of perivascular inflammatory infiltrates and blood-brain barrier disturbance in multiple sclerosis...
  81. ncbi Altered fibrin architecture is associated with hypofibrinolysis and premature coronary atherothrombosis
    J P Collet
    Department of Cardiology, Pitie Salpetriere University Hospital, Paris, France
    Arterioscler Thromb Vasc Biol 26:2567-73. 2006
    Hypofibrinolysis promotes atherosclerosis progression and recurrent ischemic events in premature coronary artery disease. We investigated the role of fibrin physical properties in this particular setting.
  82. ncbi Hematopoietic cell-derived microparticle tissue factor contributes to fibrin formation during thrombus propagation
    Janet Chou
    Center for Hemostasis and Thrombosis Research, Vascular Biology Center, 330 Brookline Ave, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA
    Blood 104:3190-7. 2004
    ..Platelet, TF, and fibrin accumulation in the developing thrombus was compared in wild-type and low TF mice...
  83. ncbi Influence of fibrin network conformation and fibrin fiber diameter on fibrinolysis speed: dynamic and structural approaches by confocal microscopy
    J P Collet
    Department of Cardiology, Hemostasis Laboratory, Pitie Salpetriere Hospital, Hotel Dieu Hospital, Paris, France
    Arterioscler Thromb Vasc Biol 20:1354-61. 2000
    Abnormal fibrin architecture is thought to be a determinant factor of hypofibrinolysis...
  84. pmc Fibrin derived from patients with chronic thromboembolic pulmonary hypertension is resistant to lysis
    Timothy A Morris
    Division of Pulmonary Critical Care Medicine, Department of Medicine, University of California, San Diego, CA 92103 8378, USA
    Am J Respir Crit Care Med 173:1270-5. 2006
    ....
  85. pmc Fibrin gels and their clinical and bioengineering applications
    Paul A Janmey
    Department of Physiology, Institute for Medicine and Engineering, University of Pennsylvania, 3340 Smith Walk, Philadelphia, PA 19104, USA
    J R Soc Interface 6:1-10. 2009
    b>Fibrin gels, prepared from fibrinogen and thrombin, the key proteins involved in blood clotting, were among the first biomaterials used to prevent bleeding and promote wound healing...
  86. ncbi Plasminogen deficiency causes severe thrombosis but is compatible with development and reproduction
    T H Bugge
    Division of Basic Science Research, Children s Hospital Research Foundation, Cincinnati, Ohio 45229, USA
    Genes Dev 9:794-807. 1995
    ..b>Fibrin deposition in the liver was a uniform finding in 5- to 21-week-old mice, and ulcerated lesions in the ..
  87. doi Tenascin-C triggers fibrin accumulation by downregulation of tissue plasminogen activator
    Florence Brellier
    Friedrich Miescher Institute for Biomedical Research, Novartis Research Foundation, Basel, Switzerland
    FEBS Lett 585:913-20. 2011
    ..In agreement with these observations, tenascin-C deficient MEFs had an increased capacity to digest fibrin in situ...
  88. pmc The interplay between tissue plasminogen activator domains and fibrin structures in the regulation of fibrinolysis: kinetic and microscopic studies
    Colin Longstaff
    National Institute for Biological Standards and Control, South Mimms, UK
    Blood 117:661-8. 2011
    Regulation of tissue-type plasminogen activator (tPA) depends on fibrin binding and fibrin structure...
  89. ncbi Mechanical properties and remodeling of hybrid cardiac constructs made from heart cells, fibrin, and biodegradable, elastomeric knitted fabric
    Jan Boublik
    Division of Health Sciences and Technology, Center for Space Research, and Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, 02139, USA
    Tissue Eng 11:1122-32. 2005
    ..suitable for in vitro loading studies and in vivo implantation were constructed from neonatal rat heart cells, fibrin (Fn), and biodegradable knitted fabric (Knit)...
  90. doi Cancer as an overhealing wound: an old hypothesis revisited
    Matthias Schafer
    Institute of Cell Biology, ETH Zurich, Schafmattstrasse 18, 8093 Zurich, Switzerland
    Nat Rev Mol Cell Biol 9:628-38. 2008
    ..However, a few crucial differences were also observed, which could account for the altered metabolism, impaired differentiation capacity and invasive growth of malignant tumours...
  91. ncbi Mesenchymal stem cells enhance angiogenesis in mechanically viable prevascularized tissues via early matrix metalloproteinase upregulation
    Cyrus M Ghajar
    Department of Biomedical Engineering, University of California Irvine, Irvine, California 92697 2575, USA
    Tissue Eng 12:2875-88. 2006
    ..In this study, the ability of endothelial cells to sprout and form stable vascular networks in 3-dimensional (3D) fibrin matrices was investigated as a function of matrix density in a prevascularized tissue model...
  92. ncbi Covalently conjugated VEGF--fibrin matrices for endothelialization
    A H Zisch
    Department of Materials and Institute for Biomedical Engineering, ETH and University of Zurich, Moussonstrasse 18, 8044 Zurich, Switzerland
    J Control Release 72:101-13. 2001
    ..The objective of this study was to confer the angiogenic activity of VEGF(121) upon the biomaterial fibrin, a natural substrate for endothelial cell growth and clinically accepted as 'fibrin glue'...
  93. ncbi Fibrinogen and fibrin
    R F Doolittle
    Annu Rev Biochem 53:195-229. 1984
  94. ncbi The nine residue plasminogen-binding motif of the pneumococcal enolase is the major cofactor of plasmin-mediated degradation of extracellular matrix, dissolution of fibrin and transmigration
    Simone Bergmann
    Research Center for Infectious Diseases, University of Wurzburg, Germany
    Thromb Haemost 94:304-11. 2005
    ..In particular, bacterial cell enolase-bound plasmin potentiated dissolution of fibrin or laminin and transmigration of pneumococci through a fibrin matrix...
  95. pmc Crystal structure of the complex between thrombin and the central "E" region of fibrin
    Igor Pechik
    Jerome H Holland Laboratory for the Biomedical Sciences, American Red Cross, 15601 Crabbs Branch Way, Rockville, MD 20855, USA
    Proc Natl Acad Sci U S A 101:2718-23. 2004
    Nonsubstrate interactions of thrombin with fibrin play an important role in modulating its procoagulant activity...
  96. doi Iron enhances generation of fibrin fibers in human blood: implications for pathogenesis of stroke
    Boguslaw Lipinski
    Joslin Diabetes Center, Harvard Medical School, Boston, MA 02215, USA
    Microsc Res Tech 75:1185-90. 2012
    Stroke is associated with the intracerebral formation of fibrin clots which may lead to irreversible brain damage...
  97. doi Improvement of spatial fibrin formation by the anti-TFPI aptamer BAX499: changing clot size by targeting extrinsic pathway initiation
    L A Parunov
    Center for Theoretical Problems of Physicochemical Pharmacology, Moscow, Russia
    J Thromb Haemost 9:1825-34. 2011
    ..However, it is not clear how stimulation of coagulation onset by inactivating TFPI will affect spatial and temporal clot propagation...
  98. doi Delayed arterial healing and increased late stent thrombosis at culprit sites after drug-eluting stent placement for acute myocardial infarction patients: an autopsy study
    Gaku Nakazawa
    CVPath Institute, Inc, 19 Firstfield Rd, Gaithersburg, MD 20878, USA
    Circulation 118:1138-45. 2008
    ..Using autopsy data, we evaluated the pathological responses of the stented segment in patients treated with DES for AMI and compared with patients with stable angina...
  99. ncbi Leukocyte- and platelet-rich fibrin (L-PRF) for long-term delivery of growth factor in rotator cuff repair: review, preliminary results and future directions
    Matthias A Zumstein
    Orthopaedic Sports Medicine and Knee Surgery, Dept of Orthopaedic Surgery and Traumatology, Inselspital University of Bern, 3010 Bern, Switzerland
    Curr Pharm Biotechnol 13:1196-206. 2012
    ..Subsequently, this provisional matrix is replaced by the definitive matrix. Leukocyte- and platelet-rich fibrin (L-PRF) contain growth factors and has a stable dense fibrin matrix...
  100. ncbi The influence of type 2 diabetes on fibrin structure and function
    E J Dunn
    Academic Unit of Molecular Vascular Medicine, Faculty of Medicine and Health, Leeds Institute for Genetics, Health and Therapeutics, University of Leeds, G Floor, Martin Wing, Leeds General Infirmary, Leeds, LS1 3EX, UK
    Diabetologia 48:1198-206. 2005
    ..The precise mechanisms underlying the increased risk of cardiovascular disease (CVD) in type 2 diabetes are unclear. Fibrin clot structure has been related to CVD risk in the general population...
  101. doi The effect of platelet-rich fibrin matrix on rotator cuff tendon healing: a prospective, randomized clinical study
    Scott A Rodeo
    The Hospital for Special Surgery, 535 East 70th Street, New York, NY 10021, USA
    Am J Sports Med 40:1234-41. 2012
    ..There is a strong need for methods to improve the biological potential of rotator cuff tendon healing. Platelet-rich fibrin matrix (PRFM) allows delivery of autologous cytokines to healing tissue, and limited evidence suggests a positive ..

Research Grants71

  1. Clinical Development of Hemostatic Agent ClotFoam for Severe Hemorrhage
    Maja Nowakowski; Fiscal Year: 2013
    ..ClotFoam(R) is also a plasmin-depleted aprotinin-and-thrombin-free fibrin sealant presenting no risk of autoimmune reaction to blood products...
  2. The Role of Carboxypeptidase B and its Substrates in Rheumatoid Arthritis
    William H Robinson; Fiscal Year: 2013
    ..histologic features in RA joints are synovial hyperplasia, inflammatory cell infiltrates, bone erosions and fibrin deposits...
  3. Thiol Isomerases During Thrombus Formation
    Barbara C Furie; Fiscal Year: 2013
    ..activation monitored by calcium mobilization, platelet alpha granule release detected by P-selectin expression, fibrin formation, thrombin activity, platelet accumulation, platelet on/off rates, platelet thrombus embolization, ..
  4. Novel therapeutics for vesicants and toxic inhaled chemicals (U54)
    Carl W White; Fiscal Year: 2013
    ..In lung, CEES caused bronchial vascular injury/leak. Airway obstructive fibrin-containing casts formed after inhalation exposures to CEES or SM, and similar lesions were apparent after chlorine ..
  5. STRUCTURE AND FUNCTION OF HEPARIN COFACTOR II
    Douglas M Tollefsen; Fiscal Year: 2010
    ..Rates of accumulation of fibrin(ogen), platelets, smooth muscle cells, and macrophages in these lesions will be determined in HCII-/- and wild-..
  6. The Role of Vascular Innervation on Tissue-type Plasminogen Activator Release
    JAMES ANTHONY SHEERIN MULDOWNEY; Fiscal Year: 2013
    ..The generation, extent, and location of the fibrin clot are critical when hemostasis is compromised...
  7. Dichotomous Roles of Thrombin in Acetaminophen Hepatotoxicity
    ROBERT ANDREW ROTH; Fiscal Year: 2013
    ..resulting in activation of the thrombin receptor, protease-activated receptor-1 (PAR-1), and in the deposition of fibrin in liver...
  8. Retinal Imaging of Prognostic Indicators of Atherosclerosis
    Frederick R Haselton; Fiscal Year: 2010
    ..use QD-antibody conjugates injected in vivo to detect the inflammatory markers VCAM-1, MCP-1, MSR, and dimerized fibrin (D-dimer) on plaque surfaces, which serve as relatively early and late indicators of atherosclerotic disease...
  9. PET Imaging of Thrombus
    PETER D CARAVAN; Fiscal Year: 2013
    ..The goal of this proposal is to develop a fibrin-specific positron emission tomography (PET) probe for direct imaging of thrombus...
  10. Clinical trial of self complementary AAV8-mediated gene transfer for hemophilia B
    Andrew M Davidoff; Fiscal Year: 2013
    ..that results from a defect in the factor IX (FIX) gene which encodes a serine protease critical for appropriate fibrin clot formation...
  11. Stem Cells For Myocardial Repair: A Large Bore Magnet Study
    Jianyi Zhang; Fiscal Year: 2012
    ..This proposal will examine whether a PEGylated fibrin patch based stem cell transplantation can provide a new regeneration therapy for heart failure patients.
  12. PLASMINOGEN AND PLASMIN: STRUCTURE AND FUNCTION
    Francis J Castellino; Fiscal Year: 2013
    ..their ability to bind to host human plasminogen (hPg) directly (PAM), or, indirectly (M1), via fibrinogen (Fg)/fibrin (Fn)...
  13. Coagulation and Fibrinolysis in Pediatric Insulin Titration Trial
    Anil Sapru; Fiscal Year: 2013
    ..This pro-thrombotic, anti-fibrinolytic state, may lead to intravascular fibrin deposition and micro thrombi, which can be a key contributor to the pathogenesis of multi-organ failure...
  14. Mesenchymal Stem Cells To Mitigate and Treat Cutaneous Injury Following Radiation
    William H McBride; Fiscal Year: 2010
    ..sources based on the finding that they adhere to multiple homologous C-terminal peptides on - and ?-chains of fibrin(ogen), termed haptides...
  15. Role of influenza-induced prothrombosis in Streptococcus pyogenes superinfections
    Michael S Chaussee; Fiscal Year: 2012
    ..pyogenes superinfections. Influenza infection induces thrombosis, which results in fibrinogen/fibrin (Fb) and fibronectin (Fn) deposition in the respiratory tract. These ae well-known ligands involved in S...
  16. High throughput assay for fibrin-binding probes
    PETER D CARAVAN; Fiscal Year: 2013
    ..provided by applicant): The goal of this application is to develop a high throughput screening assay to identify fibrin-specific compounds...
  17. Mini-plasmin: Pre-Clinical Evaluation of a Novel Thrombolytic Strategy for Stroke
    Irina Y Sazonova; Fiscal Year: 2012
    ..The fundamental mechanism of ischemic stroke is the occlusion of a cerebral vessel by a fibrin rich blood clot (thrombus)...
  18. Factor XI in Thrombosis
    David Gailani; Fiscal Year: 2013
    ..regarding the importance of contact activation to thrombosis in humans, we noted that fXII contributes to fibrin formation and platelet aggregation in human blood in an ex vivo flow model...
  19. EVOLVING MECHANICS OF INTRALUMINAL THROMBUS: FROM FORMATION TO ORGANIZATION
    Jay D Humphrey; Fiscal Year: 2010
    ..All past studies of the mechanical properties of blood clots have focused on either newly formed, primarily fibrin-based, clots or mature clots having an unknown natural history that were obtained from patients...
  20. A Novel Electrospun Vascular Graft
    David J Vachon; Fiscal Year: 2010
    ..These materials are prone to rapid protein (e.g. fibrinogen, fibrin) deposition, denaturation and subsequent adhesion and activation of blood platelets potentially leading to clot ..
  21. Mechanism of Staphylocoagulase-activated Blood Clotting
    Paul E Bock; Fiscal Year: 2013
    ..Active SC/vWbp.ProT* complexes cleave fibrinogen (Fbg) to fibrin (Fbn), aiding growth of platelet-Fbn-bacteria vegetations. S...
  22. Bilayered Vocal Fold Tissue Engineering
    Jennifer L Long; Fiscal Year: 2013
    ..Adult human stem cells isolated from adipose tissue are cultured within fibrin hydrogel derived from the blood product cryoprecipitate...
  23. CYTOKINE REGULATION OF RA SYNOVIOCYTE PHENOTYPE
    Lionel B Ivashkiv; Fiscal Year: 2013
    ..We have found that fibrin(ogen) and immune complexes, two factors expressed at high levels in RA synovium and implicated in inflammatory ..
  24. Deranged Coagulation and Fibrinolytic Cascades in Idiopathic Pulmonary Fibrosis
    MITCHELL ALAN OLMAN; Fiscal Year: 2013
    ..Furthermore, plasma fibrin D dimer levels from a small anti-coagulant trial were increased during an acute exacerbation, and those with ..
  25. Impaired integrin-dependent function of WASP-deficient platelets
    EILEEN REMOLD-O'DONNELL; Fiscal Year: 2010
    ..of FITC-lactadherin (aim 2a), rebleeding in a mouse tail cut model to assess clot stabilization (aim 2b) and fibrin clot retraction (aim 2c)...
  26. Hemostatic-antibiotic Combination for Prevention of MRSA Surgical Site Infections
    Timothy C Fisher; Fiscal Year: 2010
    ..g., thrombin, collagen, fibrin)...
  27. A diagnostic instrument to manage hemostasis in chronic liver disease
    Francesco Viola; Fiscal Year: 2012
    ..factors) but also clot formation rate (also dependent upon coagulation factors), clot stiffness (dependent upon fibrin assembly and platelet function), and time to lysis (dependent upon fibrinolytic proteins)...
  28. Association of TFPI with antemortem dementia and postmortem micro brain infarcts
    SUSAN ANTONE MARONEY; Fiscal Year: 2013
    ..Mouse models of either tfpi-/- and pros-/- mice die in utero and have intravascular fibrin clots in the brain...
  29. Reduction of Tunneled Dialysis Catheter Dysfunction Via Long Term Nitric Oxide Re
    Prabir Roy-Chaudhury; Fiscal Year: 2013
    ..cell-proliferation activities to target all the current effector arms of TDC dysfunction: infection thrombosis/fibrin sheath formation, and central venous stenosis...
  30. Structural origin of fibrin clot mechanical properties
    John W Weisel; Fiscal Year: 2012
    ..provided by applicant): Although we know a great deal about the structure and many aspects of the functions of fibrin(ogen), we still know very little about the microscopic and molecular structural origins of the fibrin clot's ..
  31. Role of the Plasminogen Activator Protease during Pneumonic Plague
    Wyndham W Lathem; Fiscal Year: 2013
    ..In addition, there exists an intimate link between fibrin deposition and inflammation...
  32. Mechanisms linking the hemostatic protease thrombin to arthritic disease
    Matthew J Flick; Fiscal Year: 2013
    ..Robust fibrin deposition within affected joints is one pathological consequence of exuberant thrombin activity, and fibrin(ogen) ..
  33. Assessment of Corneal Fibroblast Biomechanical Behavior
    W Matthew Petroll; Fiscal Year: 2013
    ..for the first time that whereas corneal fibroblasts generally move independently within collagen matrices, fibrin induces a switch to an interconnected, collective mode of cell spreading and migration, which is associated with ..
  34. Engineering fibrin polymers for enhanced angiogenesis
    THOMAS HARRISON BARKER; Fiscal Year: 2013
    ..A fibrin polymer is capable of engaging many integrins on mostly inflammatory cells;however the lack of specificity with ..
  35. Pulmonary Formulation of tPA for Plastic Bronchitis
    Kathleen A Stringer; Fiscal Year: 2010
    ..by applicant): Plastic bronchitis (PB) is a rare pediatric disease characterized by the presence of large fibrin or mucin casts in the airways...
  36. Regulation of Protease-activated Receptor-1 Signaling
    JoAnn Trejo; Fiscal Year: 2012
    ..Thrombin drives fibrin deposition and activates platelet, which are critical for hemostasis and thrombosis...
  37. MOLECULAR MECHANISMS OF FIBRINOLYSIS
    Paul E Bock; Fiscal Year: 2013
    ..By mechanisms that are not understood, Pm degrades protective fibrin (Fbn) barriers generated by the host response...
  38. CHADSS: Chagas Disease Scan Study
    Jamary Oliveira-Filho; Fiscal Year: 2013
    ..inflammation can result in endothelial dysfunction and stimulate the hemostatic system, increasing systemic fibrin production and platelet activation. Brain atrophy has also been associated with chronic inflammation...
  39. Pathobiology of Staphyloccocus areus Endocarditis
    PETER ROLF PANIZZI; Fiscal Year: 2010
    ..recognition of fibrinogen by the active staphylocoagulase-ProT complex resulting in conversion of fibrinogen to fibrin. Fibrin generation is hypothesized to play a critical role in ABE pathology...
  40. Multiscale, Multiphysics Model of Thrombus Biomechanics in Aortic Dissection
    George Karniadakis; Fiscal Year: 2013
    ..Specifically, we will extend and then couple a multiscale model of blood flow, platelet kinetics, fibrin organization, and plasmin transport (Karniadakis group) with a multiscale model of aortic wall mechanics and ..
  41. The Role of Tfam in Mitochondrial Biogenesis following sepsis
    Jeffrey Keenan; Fiscal Year: 2013
    ..Sepsis will then be induced by the peritoneal implantation of a S. aureus impregnated fibrin-clot in experimental and wild-type control mice...
  42. Deep Vein Thrombosis and Chronic Thrombotic Venous Disease
    Timothy A Morris; Fiscal Year: 2012
    ..Specifically, their fibrin tends to form less turbid clots, composed of thinner fibers that may enhance the accessibility of epitopes that ..
  43. Platelet adhesion to neutrophil extracellular DNA traps: role in thrombosis
    Denisa D Wagner; Fiscal Year: 2013
    ..The central hypothesis of this application is that NETs provide a scaffold that, together with fibrin, increases thrombus stability and promotes thrombus growth and microparticle recruitment...
  44. Development of B-Lock an antibiofilm catheter lock product
    Krzysztof Appelt; Fiscal Year: 2010
    ..Biofilms formed on catheters are complex structures composed of host-derived fibrin deposits and stranding as well as pathogen- derived, extracellular polysaccharide-rich matrix...
  45. Computer Integrated Systems for Microscopy and Manipulation
    Richard Superfine; Fiscal Year: 2013
    ..They cover length scales ranging from single molecule (mucin, myosin, MutS) to macromolecular complexes (fibrin fibers, viruses), cells (Plexin, cell motility, cell division), tissue cultures (human lung cell cultures) and ..
  46. Mechanisms and functions of p75 neurotrophin receptor signaling in astrocytes
    Katerina Akassoglou; Fiscal Year: 2013
    ..We have shown that the neurotrophin receptor p75NTR regulates repair processes by inhibiting fibrin degradation and regulating cell differentiation...
  47. Pathobiology of Staphyloccocus areus Endocarditis
    PETER ROLF PANIZZI; Fiscal Year: 2013
    ..recognition of fibrinogen by the active staphylocoagulase-ProT complex resulting in conversion of fibrinogen to fibrin. Fibrin generation is hypothesized to play a critical role in ABE pathology...
  48. Transscleral Drug Delivery for Retinal Disorders
    HENRY FRANCIS EDELHAUSER; Fiscal Year: 2010
    ..We propose to develop novel transscleral drug delivery approaches that use nanoparticles, microneedles, fibrin sealant, ontophoresis, and electoporation...
  49. Reducing Mortality from Acute Hemorrhage in Trauma
    RHONDA K COOKE; Fiscal Year: 2012
    ..The clinical trials we proposed in the initial period addressed the use of rVlla and the dry fibrin sealant dressing for controlling major hemorrhage...
  50. Transglutaminase-Based Imaging of Wound Healing
    Gregory W Faris; Fiscal Year: 2013
    ..Wound healing involves an intricate set of precisely timed processes that begin with the formation of a fibrin clot, followed by an inflammatory response, and eventually ending with extensive tissue remodeling and wound ..
  51. Hemostatic factors and sickle cell disease
    Punam Malik; Fiscal Year: 2013
    ..of thrombin in hemostasis and vascular biology is underscored by the fact that this protease positively controls fibrin deposition, platelet activation, and endothelial cell (EC) signaling events via multiple substrates and receptors [..
  52. 3-D microtissues with perfused human capillaries
    STEVEN CARL GEORGE; Fiscal Year: 2010
    ..an arteriole and venule, separated by a third central parallel channel that contains stromal cells embedded in fibrin. The channels are filled with flowing media enriched with oxygen and other nutrients, and are porous at fixed ..
  53. Enhanced Oral Tissue Repair via Self Assembled Polymer Multi-layer Barriers for t
    THOMAS DEAN DZIUBLA; Fiscal Year: 2010
    ..by rinsing the oral cavity with an aqueous polymeric solution, which can self assemble at the site of injury via fibrin targeting, it is possible to form a barrier that act as a platform for the sustained antioxidant therapy improving ..
  54. STEP UP In Health-related Research
    Raul DeLa Cadena; Fiscal Year: 2013
    ..Center which focuses on research related to platelet function, blood coagulation, neutrophil activation and fibrin formation, 2) the Cardiovascular Research Center with research related to the function of the cardiovascular ..
  55. Mechanisms of Homocysteine-Induced Fibrinogen-Amyloid Plaque Formation
    Suresh C Tyagi; Fiscal Year: 2013
    ..While HHcy increases MMP-9 activity and enhances the paracellular transport as well as homocyteinylates Fg (making fibrin clots more rigid), Fg enhances the transcellular transport through caveolin-1 (Cav-1) signaling (via ..
  56. Purinergic Thromboregulation
    Simon C Robson; Fiscal Year: 2010
    ..of CD39, CD39L1 and CD39L3 in generated thrombi, with platelet sequestration, associated tissue factor and fibrin deposition. b) Contrast the impact of vascular Cd39L1 and/or Cd39L3 deletion vs...
  57. Mechanism of Staphylocoagulase-activated Blood Clotting
    Paul E Bock; Fiscal Year: 2012
    ..ProT) non-proteolytically and forms an SOProT complex that specifically converts fibrinogen (Fbg) into fibrin. The studies focus on 6 areas: (1) Structure-function relationships governing the critical SC N-terminal insertion ..
  58. DEVELOPMENT OF AN IN VITRO WOUND HEALING MODEL
    Frederick Grinnell; Fiscal Year: 2012
    ..proposal, we plan to analyze human fibroblast migration and collagen flow in nested collagen matrices and in fibrin matrices...
  59. Novel antithrombotic agents to prevent hemodialysis vascular access failure
    TZE CHEIN WUN; Fiscal Year: 2013
    ..shear stress, the foreign graft material and uremia can elicit a strong thrombotic response that lays down a mural fibrin- and platelet-rich substrate on which excessive cell migration, proliferation and matrix deposition occur, leading ..
  60. Annexin A2 and Protein p11 in Endotoxemia and Sepsis
    KATHERINE AMBERSON HAJJAR; Fiscal Year: 2013
    ..In preliminary studies, we have found that while A2-/- mice have greater accumulations of microvascular fibrin during endotoxemia, they have unexpectedly reduced levels of tissue inflammation, despite extremely elevated ..
  61. Role of Fibrinogen in Alzheimer's Disease
    Sidney Strickland; Fiscal Year: 2013
    ..an increase in blood brain barrier (BBB) permeability and neurovascular damage in AD mice, and we showed that fibrin(ogen) deposition potentiates these processes...
  62. Efficacy of Kunitz Domain 1 variants of TFPI-2 as novel antifibrinolytic agents
    MADHU SATYA BAJAJ; Fiscal Year: 2010
    ..In patients with open-heart surgery and organ transplant, there is significant blood loss due to breakdown of fibrin by plasmin...
  63. ENDOTHELIAL INJURY AND CYTOPROTECTION
    JACK HAWIGER; Fiscal Year: 1993
    This proposal is focused on the study of two key changes involved in the seeding of fibrin thrombi on the vessel wall a process contributing to an estimated 5 million episodes of venous thrombosis and 1...
  64. HEMOSTATIC EFFECTS OF FIBRINOLYTIC THERAPY
    BARBARA ALEVRIADOU; Fiscal Year: 2000
    ..Bach agent results in cleavage of plasminogen to plasmin. Plasmin degrades the fibrin strands and dissolves thrombi...
  65. ENVIRONMENTAL MODULATION OF FIBRINOLYSIS INHIBITORS
    STEVEN GONIAS; Fiscal Year: 1992
    Fibrinolysis is the enzyme system that eliminates existing fibrin blood clots and inhibits the deposition of new fibrin in normal blood vessels. Plasmin is the primary proteinase responsible for fibrin digestion in vivo...
  66. Enhanced Cryoprecipiate in Wound Healing
    Edward Shanbrom; Fiscal Year: 2007
    unreadable] DESCRIPTION (provided by applicant): In wound repair, fibrin has a multiplicity of activities, some of which are intrinsic to the protein itself and some attributable to other blood constituents associated with the fibrin ..
  67. ROLE OF FACTOR XIII BINDING TO THE HEMOSTATIC PLUG
    Charles Greenberg; Fiscal Year: 1993
    ..The focus of this grant will be on the interaction between FXIII with fibrin and platelets in the hemostatic plug...
  68. Physiological Role of Fibrin Anti-Thrombin I Activities
    MICHAEL MOSESSON; Fiscal Year: 2005
    ..at its substrate recognition sites in the central E domain leads to proteolytic conversion of fibrinogen to fibrin. Fibrin itself has an appreciable non-substrate thrombin binding potential termed 'anti-thrombin I', that is ..
  69. FIBRINOGEN--ROLE OF THE GAMMA VARIANT
    David Farrell; Fiscal Year: 2001
    ..This proposal addresses the issue of fibrin clot strength, a critical factor in the outcome of fibrinolytic therapy...
  70. BASIC AND CLINICAL STUDIES OF COAGULATION PROTEINS
    Charles Francis; Fiscal Year: 2003
    ..investigators to study basic and clinical aspects of hemostasis and vascular biology with a focus on fibrinogen, fibrin and the vessel wall...
  71. Hemostatic Factors and Tumor Biology
    Joseph Palumbo; Fiscal Year: 2007
    ..will use gene-targeting technology and in vivo murine tumor models to define the role of platelet activation, fibrin(ogen) and platelet/fibrin interactions in metastasis and natural killer cell mediated tumor surveillance...