colicins

Summary

Summary: Bacteriocins elaborated by strains of Escherichia coli and related species. They are proteins or protein-lipopolysaccharide complexes lethal to other strains of the same species.

Top Publications

  1. pmc Colicin biology
    Eric Cascales
    Laboratoire d Ingénierie des Systèmes Macromoléculaires, Institut de Biologie Structurale et Microbiologie, Centre National de la Recherche Scientifique, UPR 9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Microbiol Mol Biol Rev 71:158-229. 2007
  2. ncbi Bacteriocins: evolution, ecology, and application
    Margaret A Riley
    Department of Ecology and Evolutionary Biology, Yale University, New Haven, Connecticut 06511, USA
    Annu Rev Microbiol 56:117-37. 2002
  3. pmc Low resolution structure and dynamics of a colicin-receptor complex determined by neutron scattering
    Luke A Clifton
    ISIS Spallation Neutron Source, Rutherford Appleton Laboratory, Harwell Science and Innovation Campus, Didcot, Oxfordshire OX11 0QX, United Kingdom
    J Biol Chem 287:337-46. 2012
  4. pmc Allosteric beta-propeller signalling in TolB and its manipulation by translocating colicins
    Daniel A Bonsor
    Department of Biology, University of York, York, UK
    EMBO J 28:2846-57. 2009
  5. ncbi Local dispersal promotes biodiversity in a real-life game of rock-paper-scissors
    Benjamin Kerr
    Department of Biological Sciences, Stanford University, Stanford, California 94305, USA
    Nature 418:171-4. 2002
  6. ncbi Border crossings: colicins and transporters
    Karen S Jakes
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Annu Rev Genet 46:209-31. 2012
  7. pmc Structure of colicin I receptor bound to the R-domain of colicin Ia: implications for protein import
    Susan K Buchanan
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    EMBO J 26:2594-604. 2007
  8. ncbi Swimming against the tide: progress and challenges in our understanding of colicin translocation
    Colin Kleanthous
    Department of Biology, University of York, Wentworth Way, Heslington, YO10 5DD, UK
    Nat Rev Microbiol 8:843-8. 2010
  9. pmc Competitive interactions in Escherichia coli populations: the role of bacteriocins
    Hadeel Majeed
    Zuckerberg Institute for Water Research, J Blaustein Institutes for Desert Research, Ben Gurion University, Sde Boqer Campus, Israel
    ISME J 5:71-81. 2011
  10. ncbi Structure of the complex of the colicin E2 R-domain and its BtuB receptor. The outer membrane colicin translocon
    Onkar Sharma
    Department of Biological Sciences, Lilly Hall of Life Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    J Biol Chem 282:23163-70. 2007

Research Grants

  1. GATING OF PROTEIN CHANNELS IN LIPID BILAYER MEMBRANES
    Alan Finkelstein; Fiscal Year: 2013
  2. Translocation through TonB-dependent transporters
    Karen Jakes; Fiscal Year: 2013
  3. MOLECULAR ANALYSIS OF FERRIC ENTEROBACTIN TRANSPORT
    MARK MCINTOSH; Fiscal Year: 1993
  4. STRUCTURE OF BACTERIAL MEMBRANE PROTEINS
    R Garavito; Fiscal Year: 1992
  5. FLUID BILAYER INTERACTIONS WITH PEPTIDES
    Stephen White; Fiscal Year: 2004
  6. MOLECULAR BASIS OF MEMBRANE EXCITATION
    Wayne Hubbell; Fiscal Year: 1999
  7. Receptor-mediated Colicin Import
    WILLIAM CRAMER; Fiscal Year: 2007
  8. Voltage-Gated Insertion of Colicin into Planar Bilayers
    WILLIAM CRAMER; Fiscal Year: 2005
  9. EXTRA-CHROMOSOMAL GENETIC ELEMENTS AND THE CELL SURFACE
    DONNA DUCKWORTH; Fiscal Year: 1980
  10. GENETIC ANALYSIS OF THE RECA PROTEIN OF E. COLI
    George Weinstock; Fiscal Year: 1991

Detail Information

Publications241 found, 100 shown here

  1. pmc Colicin biology
    Eric Cascales
    Laboratoire d Ingénierie des Systèmes Macromoléculaires, Institut de Biologie Structurale et Microbiologie, Centre National de la Recherche Scientifique, UPR 9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Microbiol Mol Biol Rev 71:158-229. 2007
    b>Colicins are proteins produced by and toxic for some strains of Escherichia coli. They are produced by strains of E. coli carrying a colicinogenic plasmid that bears the genetic determinants for colicin synthesis, immunity, and release...
  2. ncbi Bacteriocins: evolution, ecology, and application
    Margaret A Riley
    Department of Ecology and Evolutionary Biology, Yale University, New Haven, Connecticut 06511, USA
    Annu Rev Microbiol 56:117-37. 2002
    ..In the latter half of this review we focus on the potential role bacteriocins may play in addressing human health concerns and the current role they serve in food preservation...
  3. pmc Low resolution structure and dynamics of a colicin-receptor complex determined by neutron scattering
    Luke A Clifton
    ISIS Spallation Neutron Source, Rutherford Appleton Laboratory, Harwell Science and Innovation Campus, Didcot, Oxfordshire OX11 0QX, United Kingdom
    J Biol Chem 287:337-46. 2012
    ..This revealed that colicin N unfolds and binds to the OmpF-lipid interface. The implications of this unfolding step for colicin translocation across membranes are discussed...
  4. pmc Allosteric beta-propeller signalling in TolB and its manipulation by translocating colicins
    Daniel A Bonsor
    Department of Biology, University of York, York, UK
    EMBO J 28:2846-57. 2009
    The Tol system is a five-protein assembly parasitized by colicins and bacteriophages that helps stabilize the Gram-negative outer membrane (OM)...
  5. ncbi Local dispersal promotes biodiversity in a real-life game of rock-paper-scissors
    Benjamin Kerr
    Department of Biological Sciences, Stanford University, Stanford, California 94305, USA
    Nature 418:171-4. 2002
    ..We find that diversity is rapidly lost in our experimental community when dispersal and interaction occur over relatively large spatial scales, whereas all populations coexist when ecological processes are localized...
  6. ncbi Border crossings: colicins and transporters
    Karen S Jakes
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Annu Rev Genet 46:209-31. 2012
    b>Colicins are protein toxins produced by Escherichia coli to kill related bacteria. They must cross the target cell outer membrane (OM), and some must also cross the inner membrane (IM)...
  7. pmc Structure of colicin I receptor bound to the R-domain of colicin Ia: implications for protein import
    Susan K Buchanan
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    EMBO J 26:2594-604. 2007
    ..Functional data using full-length colicin Ia show that colicin I receptor is necessary for cell surface binding, and suggest that the receptor participates in translocation of colicin Ia across the outer membrane...
  8. ncbi Swimming against the tide: progress and challenges in our understanding of colicin translocation
    Colin Kleanthous
    Department of Biology, University of York, Wentworth Way, Heslington, YO10 5DD, UK
    Nat Rev Microbiol 8:843-8. 2010
    b>Colicins are folded protein toxins that face the formidable task of translocating across one or both of the Escherichia coli cell membranes in order to induce cell death. This translocation is achieved by parasitizing host proteins...
  9. pmc Competitive interactions in Escherichia coli populations: the role of bacteriocins
    Hadeel Majeed
    Zuckerberg Institute for Water Research, J Blaustein Institutes for Desert Research, Ben Gurion University, Sde Boqer Campus, Israel
    ISME J 5:71-81. 2011
    ..Two Escherichia coli strains were generated, each carrying a DNA-degrading bacteriocin (colicins E2 and E7)...
  10. ncbi Structure of the complex of the colicin E2 R-domain and its BtuB receptor. The outer membrane colicin translocon
    Onkar Sharma
    Department of Biological Sciences, Lilly Hall of Life Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    J Biol Chem 282:23163-70. 2007
    ..It was concluded that the oblique orientation of the R-domain of the nuclease E colicins has a function in the recruitment of another member(s) of an outer membrane translocon...
  11. ncbi Antibiotic-mediated antagonism leads to a bacterial game of rock-paper-scissors in vivo
    Benjamin C Kirkup
    Department of Ecology and Evolutionary Biology, Yale University, New Haven, Connecticut 06511, USA
    Nature 428:412-4. 2004
    b>Colicins are narrow-spectrum antibiotics produced by and active against Escherichia coli and its close relatives...
  12. pmc Colicin E2 is still in contact with its receptor and import machinery when its nuclease domain enters the cytoplasm
    Denis Duché
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Biologie Structural et Microbiologie, CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Bacteriol 189:4217-22. 2007
    b>Colicins reach their targets in susceptible Escherichia coli strains through two envelope protein systems: the Tol system is used by group A colicins and the TonB system by group B colicins...
  13. pmc The colicin Ia receptor, Cir, is also the translocator for colicin Ia
    Karen S Jakes
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Mol Microbiol 75:567-78. 2010
    ..for the colicin to cross the outer membrane, but no 'second receptor' has ever been identified for TonB-dependent colicins, such as Ia...
  14. ncbi Nuclease colicins and their immunity proteins
    Grigorios Papadakos
    Department of Biology, University of York, Wentworth Way, Heslington, York YO10 5DD, UK
    Q Rev Biophys 45:57-103. 2012
    ..coli, known collectively as nuclease colicins, and the specific immunity (inhibitor) proteins that colicin-producing organisms make in order to avoid ..
  15. pmc Mobility of BtuB and OmpF in the Escherichia coli outer membrane: implications for dynamic formation of a translocon complex
    Jeff Spector
    Department of Physics, Purdue University, West Lafayette, Indiana, USA
    Biophys J 99:3880-6. 2010
    ..vitamin B12) receptor (BtuB) and the OmpF porin, which are implicated in the cellular import pathways of colicins and phages-was measured in vivo...
  16. ncbi Structural and mechanistic basis of immunity toward endonuclease colicins
    C Kleanthous
    School of Biological Sciences, University of East Anglia, Norwich, UK
    Nat Struct Biol 6:243-52. 1999
    ..This hyperefficient mechanism of inhibition could be well suited to other toxic enzyme systems, particularly where the substrate is a polymer extending beyond the boundaries of the active site...
  17. ncbi Structural aspects of the inhibition of DNase and rRNase colicins by their immunity proteins
    Olatomirin O Kolade
    Schools of Biological and Chemical Sciences, University of East Anglia, Norwich, NR4 7TJ, Norfolk, UK
    Biochimie 84:439-46. 2002
    Nuclease E colicins that exert their cytotoxic activity through either non-specific DNase or specific rRNase action are inhibited by immunity proteins in a high affinity interaction that gives complete protection to the producing host ..
  18. pmc Genome-wide screens: novel mechanisms in colicin import and cytotoxicity
    Onkar Sharma
    Department of Biological Sciences, Lilly Hall of Life Sciences, Purdue University, West Lafayette, IN 47907, USA
    Mol Microbiol 73:571-85. 2009
    ..Genome-wide screening using the 'Keio collection' to test sensitivity to colicins (col) A, B, D, E1, E2, E3, E7 and N from groups A and B, allowed identification of novel genes affecting ..
  19. pmc Structure and function of colicin S4, a colicin with a duplicated receptor-binding domain
    Thomas Arnold
    Department I, Protein Evolution, Max Planck Institute for Developmental Biology, Spemannstrasse 35, 72076 Tubingen, Germany
    J Biol Chem 284:6403-13. 2009
    b>Colicins are plasmid-encoded toxic proteins produced by Escherichia coli strains to kill other E. coli strains that lack the corresponding immunity protein...
  20. ncbi The cytotoxic domain of colicin E9 is a channel-forming endonuclease
    Khedidja Mosbahi
    School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, UK
    Nat Struct Biol 9:476-84. 2002
    ..Our data suggest that endonuclease domains of colicins may mediate their own translocation across the bacterial inner membrane through an intrinsic channel activity ..
  21. ncbi Crystal structure of colicin E3: implications for cell entry and ribosome inactivation
    S Soelaiman
    Department of Biochemistry, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
    Mol Cell 8:1053-62. 2001
    b>Colicins kill E. coli by a process that involves binding to a surface receptor, entering the cell, and, finally, intoxicating it. The lethal action of colicin E3 is a specific cleavage in the ribosomal decoding A site...
  22. pmc Enhancement of Shiga toxin production in enterohemorrhagic Escherichia coli serotype O157:H7 by DNase colicins
    Hirono Toshima
    Graduate School of Human Life Science, Osaka City University, 3 3 138 Sugimoto, Osaka 558 8585, Japan
    Appl Environ Microbiol 73:7582-8. 2007
    b>Colicins are proteins produced by and active against several strains of Escherichia coli...
  23. ncbi High level expression of His-tagged colicin pore-forming domains and reflections on the sites for pore formation in the inner membrane
    Susan L Fridd
    School of Biochemistry and Genetics, University of upon Tyne NE2 4HH, Newcastle, UK
    Biochimie 84:477-83. 2002
    There exists ample evidence for the assumption that pore-forming colicins cannot exert their toxicity within the producing cell and that they must gain access to the outer face of the cytoplasmic membrane to achieve this...
  24. pmc DNA and amino acid sequence analysis of structural and immunity genes of colicins Ia and Ib
    J A Mankovich
    J Bacteriol 168:228-36. 1986
    The nucleotide sequences for colicin Ia and colicin Ib structural and immunity genes were determined. The two colicins each consist of 626 amino acid residues...
  25. pmc Escherichia coli bacteriocins: antimicrobial efficacy and prevalence among isolates from patients with bacteraemia
    Maruska Budic
    Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia
    PLoS ONE 6:e28769. 2011
    ..Escherichia coli produces two types of bacteriocins, colicins and microcins...
  26. ncbi The structure of BtuB with bound colicin E3 R-domain implies a translocon
    Genji Kurisu
    Department of Biological Sciences, Purdue University, Lilly Hall of Life Sciences, 915 W State St, West Lafayette, Indiana 47907 1392, USA
    Nat Struct Biol 10:948-54. 2003
    ..The translocator was concluded to be OmpF from the occlusion of OmpF channels by colicin E3...
  27. pmc Directed epitope delivery across the Escherichia coli outer membrane through the porin OmpF
    Nicholas G Housden
    Department of Biology, Area 10, and York Structural Biology Laboratory, University of York, Heslington, York YO10 5DD, United Kingdom
    Proc Natl Acad Sci U S A 107:21412-7. 2010
    ..Our study explains how colicins exploit porins to deliver epitope signals to the bacterial periplasm and, more broadly, how the inherent ..
  28. pmc Structural inhibition of the colicin D tRNase by the tRNA-mimicking immunity protein
    Marc Graille
    LEBS, CNRS, UPR 9063, Gif sur Yvette, France
    EMBO J 23:1474-82. 2004
    b>Colicins are toxins secreted by Escherichia coli in order to kill their competitors...
  29. pmc FtsH-dependent processing of RNase colicins D and E3 means that only the cytotoxic domains are imported into the cytoplasm
    Mathieu Chauleau
    CNRS, UPR 9073, Institut de Biologie Physico Chimique, 75005 Paris, France
    J Biol Chem 286:29397-407. 2011
    It has long been suggested that the import of nuclease colicins requires protein processing; however it had never been formally demonstrated...
  30. ncbi The TolA-recognition site of colicin N. ITC, SPR and stopped-flow fluorescence define a crucial 27-residue segment
    I Gokce
    Department of Chemistry Faculty of Science, Gaziomanpasa University, Tokat, Turkey
    J Mol Biol 304:621-32. 2000
    b>Colicins translocate across the Escherichia coli outer membrane and periplasm by interacting with several receptors...
  31. ncbi Purification and characterization of colicin V from Escherichia coli culture supernatants
    M J Fath
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115
    Biochemistry 33:6911-7. 1994
    ..0 amu, consistent with the mass of the unmodified 88 amino acid polypeptide. The purification scheme provides a rapid and simple way to obtain ColV for further biochemical analysis...
  32. pmc Crystal structures of the OmpF porin: function in a colicin translocon
    Eiki Yamashita
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907 1392, USA
    EMBO J 27:2171-80. 2008
    The OmpF porin in the Escherichia coli outer membrane (OM) is required for the cytotoxic action of group A colicins, which are proposed to insert their translocation and active domains through OmpF pores...
  33. pmc Energy-dependent immunity protein release during tol-dependent nuclease colicin translocation
    Mireille Vankemmelbeke
    School of Molecular Medical Sciences and Institute of Infection, Immunity, and Inflammation, Centre for Biomolecular Sciences, University of Nottingham, Nottingham NG7 2RD, United Kingdom
    J Biol Chem 284:18932-41. 2009
    Nuclease colicins bind their target receptor in the outer membrane of sensitive cells in the form of a high affinity complex with their cognate immunity proteins...
  34. ncbi Bacteriocin diversity and the frequency of multiple bacteriocin production in Escherichia coli
    David M Gordon
    School of Botany and Zoology, The Australian National University, Canberra, ACT 0200, Australia
    Microbiology 152:3239-44. 2006
    ..production of mitomycin C-inducible bacteriocins and screened using a PCR-based method for the presence of eleven colicins and seven microcins. Eight different colicins were detected and all seven microcins...
  35. ncbi Clusters in an intrinsically disordered protein create a protein-binding site: the TolB-binding region of colicin E9
    Kaeko Tozawa
    School of Chemical Sciences and Pharmacy, University of East Anglia, Norwich NR4 7TJ, United Kingdom
    Biochemistry 44:11496-507. 2005
    ....
  36. pmc Investigating early events in receptor binding and translocation of colicin E9 using synchronized cell killing and proteolytic cleavage
    Ying Zhang
    School of Molecular Medical Sciences, Institute of Infection, Immunity and Inflammation, Centre for Biomolecular Sciences, University Park, Nottingham NG7 2RD, United Kingdom
    J Bacteriol 190:4342-50. 2008
    Enzymatic colicins such as colicin E9 (ColE9) bind to BtuB on the cell surface of Escherichia coli and rapidly recruit a second coreceptor, either OmpF or OmpC, through which the N-terminal natively disordered region (NDR) of their ..
  37. ncbi The conserved asparagine in the HNH motif serves an important structural role in metal finger endonucleases
    Hsinchin Huang
    Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, ROC
    J Mol Biol 368:812-21. 2007
    ....
  38. ncbi The role of electrostatics in colicin nuclease domain translocation into bacterial cells
    Daniel Walker
    Department of Biology, University of York, York YO10 5YW, United Kingdom
    J Biol Chem 282:31389-97. 2007
    The mechanism(s) by which nuclease colicins translocate distinct cytotoxic enzymes (DNases, rRNases, and tRNases) to the cytoplasm of Escherichia coli is unknown...
  39. ncbi Identification of an essential cleavage site in ColE7 required for import and killing of cells
    Zhonghao Shi
    Institute of Biochemistry, National Yang Ming University, Taipei 11221, Taiwan and Institute of Molecular Biology, Academia Sinica, Taipei 11529, Taiwan
    J Biol Chem 280:24663-8. 2005
    ..Nuclease colicins are processed during translocation with only the cytotoxic nuclease domains traversing the inner membrane to ..
  40. pmc Characterization of colicin S4 and its receptor, OmpW, a minor protein of the Escherichia coli outer membrane
    H Pilsl
    Mikrobiologie Membranphysiologie, Universitat Tubingen, Tubingen, Germany
    J Bacteriol 181:3578-81. 1999
    ..By contrast, the predicted receptor binding domain shows no sequence similarities to other colicins. Mutants that lacked the OmpW protein were resistant to colicin S4.
  41. pmc The structural and energetic basis for high selectivity in a high-affinity protein-protein interaction
    Nicola A G Meenan
    Department of Biology, P O Box 373, University of York, York, YO10 5YW, United Kingdom
    Proc Natl Acad Sci U S A 107:10080-5. 2010
    ....
  42. pmc Human- and plant-pathogenic Pseudomonas species produce bacteriocins exhibiting colicin M-like hydrolase activity towards peptidoglycan precursors
    Hélène Barreteau
    Universite Paris Sud, Institut de Biochimie et Biophysique Moleculaire et Cellulaire, UMR 8619, Orsay F 91405, France
    J Bacteriol 191:3657-64. 2009
    ..aeruginosa strains. These proteins thus represent a new class of bacteriocins (pyocins), the first ones reported thus far in the genus Pseudomonas that target peptidoglycan metabolism...
  43. ncbi Effect of lipids with different spontaneous curvature on the channel activity of colicin E1: evidence in favor of a toroidal pore
    Alexander A Sobko
    A N Belozersky Institute of Physico Chemical Biology, Moscow State University, Moscow 119992, Russia
    FEBS Lett 576:205-10. 2004
    ..It is inferred that the toroidal pore model previously validated for small antimicrobial peptides is applicable to colicin E1, a large protein that contains ten alpha-helices in its pore-forming domain...
  44. pmc Structural basis for 16S ribosomal RNA cleavage by the cytotoxic domain of colicin E3
    C Leong Ng
    MRC Laboratory of Molecular Biology, Cambridge, UK
    Nat Struct Mol Biol 17:1241-6. 2010
    ..These residues activate the 16S rRNA for 2' OH-induced hydrolysis. Conformational changes observed for E3-rRNase, 16S rRNA and helix 69 of 23S rRNA suggest that a dynamic binding platform is required for colicin E3 binding and function...
  45. pmc Dietary inclusion of colicin e1 is effective in preventing postweaning diarrhea caused by F18-positive Escherichia coli in pigs
    S A Cutler
    Department of Animal Science, Iowa State University, Ames, USA
    Antimicrob Agents Chemother 51:3830-5. 2007
    ..The dietary inclusion of colicin E1 may be an effective alternative to conventional antibiotics in the diets of weaning pigs for the prevention of PWD caused by F18-positive enterotoxigenic E. coli...
  46. ncbi DNA binding and cleavage by the HNH homing endonuclease I-HmuI
    Betty W Shen
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave N A3 025, Seattle, WA 98109, USA
    J Mol Biol 342:43-56. 2004
    ..surface found in the I-PpoI homing endonuclease and an associated HNH/N active site found in the bacterial colicins, and a C-terminal DNA-binding domain previously observed in the I-TevI homing endonuclease...
  47. pmc Deciphering the catalytic domain of colicin M, a peptidoglycan lipid II-degrading enzyme
    Hélène Barreteau
    Universite Paris Sud 11, UMR 8619, Institut de Biochimie et Biophysique Moleculaire et Cellulaire, 91405 Orsay
    J Biol Chem 285:12378-89. 2010
    ..Most of these residues are surface-exposed and located relatively close to each other, hence suggesting they belong to the colicin M active site...
  48. pmc Crystal structure of colicin M, a novel phosphatase specifically imported by Escherichia coli
    Kornelius Zeth
    Department of Protein Evolution, Max Planck Institute for Developmental Biology, Spemannstrasse 35, Tubingen, Germany
    J Biol Chem 283:25324-31. 2008
    b>Colicins are cytotoxic proteins secreted by certain strains of Escherichia coli...
  49. ncbi Relation between tRNase activity and the structure of colicin D according to X-ray crystallography
    Shunsuke Yajima
    Department of Bioscience, Tokyo University of Agriculture, Setagaya Ku, Tokyo 156 8502, Japan
    Biochem Biophys Res Commun 322:966-73. 2004
    ..This finding, together with our refined tRNase activity experiments, indicates that the catalytic domain starting at position 595 has activity almost equivalent to that of colicin D...
  50. ncbi Colicins and microcins: the next generation antimicrobials
    Osnat Gillor
    Department of Ecology and Evolutionary Biology, Yale University, New Haven, Connecticut 06520 8106, USA
    Adv Appl Microbiol 54:129-46. 2004
  51. ncbi Insertion intermediates of pore-forming colicins in membrane two-dimensional space
    S D Zakharov
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907 1392, USA
    Biochimie 84:465-75. 2002
    ..voltage-gated ion channels by the initially soluble C-terminal channel polypeptide (CP) of the pore-forming colicins is a fruitful area for studies on membrane protein import...
  52. ncbi Thermodynamic consequences of bipartite immunity protein binding to the ribosomal ribonuclease colicin E3
    Daniel Walker
    Department of Biology, University of York, York YO10 5YW, United Kingdom
    Biochemistry 42:4161-71. 2003
    ..These observations suggest a mechanism whereby dissociation of the immunity protein prior to translocation into the target cell is facilitated by the loss of the Im3-translocation domain interaction...
  53. pmc Genetic organization of plasmid ColJs, encoding colicin Js activity, immunity, and release genes
    D Smajs
    Department of Microbiology and Molecular Genetics and Center for the Study of Emerging and Re emerging Pathogens, University of Texas Medical School, Houston, Texas 77030, USA
    J Bacteriol 183:3949-57. 2001
    ..Colicin Js was purified as an inactive fusion protein with an N-terminal histidine tag. Activity of the purified fusion form of colicin Js was restored after cleavage of the amino acids fused to the colicin Js N terminus...
  54. ncbi Multistep binding of transition metals to the H-N-H endonuclease toxin colicin E9
    Anthony H Keeble
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom
    Biochemistry 41:10234-44. 2002
    ....
  55. ncbi Intercellular communication by related bacterial protein toxins: colicins, contact-dependent inhibitors, and proteins exported by the type VI secretion system
    Volkmar Braun
    Department of Protein Evolution, Max Planck Institute for Developmental Biology, Tubingen, Germany
    FEMS Microbiol Lett 345:13-21. 2013
    ..b>Colicins, CdiA proteins, and certain type VI toxins have a modular design with the transport functions localized in the N-..
  56. pmc Cell entry mechanism of enzymatic bacterial colicins: porin recruitment and the thermodynamics of receptor binding
    Nicholas G Housden
    Department of Biology Area 10, P O Box 373, University of York, York YO10 5YW, United Kingdom
    Proc Natl Acad Sci U S A 102:13849-54. 2005
    Binding of enzymatic E colicins to the vitamin B12 receptor, BtuB, is the first stage in a cascade of events that culminate in the translocation of the cytotoxic nuclease into the Escherichia coli cytoplasm and release of its tightly ..
  57. ncbi Calorimetric dissection of colicin DNase--immunity protein complex specificity
    Anthony H Keeble
    Department of Biology Area 10, Post Office Box 373, University of York, Heslington, York YO10 5YW, United Kingdom
    Biochemistry 45:3243-54. 2006
    ..capacity (DeltaC(p)) accompanying binding of each Im protein (Im2, Im7, Im8, and Im9) to the DNase domains of colicins E2, E7, E8, and E9, in the context of both cognate and noncognate complexes...
  58. ncbi The Tol proteins of Escherichia coli and their involvement in the translocation of group A colicins
    Jean Claude Lazzaroni
    Unité de Microbiologie et Génétique, UMR5122 CNRS INSA, Universite Lyon 1, Bâtiment André Lwoff, 10, rue Dubois, 69622 Villeurbanne Cedex, France
    Biochimie 84:391-7. 2002
    ..The Tol proteins have been parasitized by group A colicins for their translocation through the cell envelope...
  59. pmc Colicins prevent colonization of urinary catheters
    Barbara W Trautner
    Department of Medicine, Infectious Diseases Section, Baylor College of Medicine, Houston, TX, USA
    J Antimicrob Chemother 56:413-5. 2005
    ..We investigated in vitro whether a colicin-expressing strain of Escherichia coli could prevent urinary catheter colonization by a colicin-susceptible, uropathgenic strain of E. coli...
  60. ncbi Cloning and characterization of the ColE7 plasmid
    K F Chak
    Department of Biochemistry, National Yang Ming Medical College, Taipei, Taiwan, ROC
    J Gen Microbiol 137:91-100. 1991
    ..Comparison of restriction maps and DNA sequence data suggests that ColE7 and ColE2 are more closely related than other E colicin plasmids...
  61. pmc Interaction of nuclease colicins with membranes: insertion depth correlates with bilayer perturbation
    Mireille Vankemmelbeke
    School of Molecular Medical Sciences, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, United Kingdom
    PLoS ONE 7:e46656. 2012
    ..Escherichia coli cell killing by nuclease colicins occurs through DNA (DNases) or RNA (RNases) hydrolysis and to this end their cytotoxic domains require ..
  62. pmc Genetics of resistance to colicins in Escherichia coli K-12: cross-resistance among colicins of group B
    J K Davies
    J Bacteriol 123:96-101. 1975
    This paper describes the isolation of mutants resistant to colicins of group B (i.e., colicins B, D, G, H, Ia, Ib, M, Q, S1, and V)...
  63. ncbi Primary events in the colicin translocon: FRET analysis of colicin unfolding initiated by binding to BtuB and OmpF
    Stanislav D Zakharov
    Department of Biological Sciences and Purdue Cancer Center, Purdue University, West Lafayette, Indiana 47907 2054, USA
    Biochemistry 47:12802-9. 2008
    ..The R-domain Arg399 is responsible for electrostatic interaction with BtuB. It is concluded that free energy for colicin unfolding is provided by binding of the R- domain to BtuB and binding/insertion of the T-domain to/into OmpF...
  64. ncbi Colicins exploit native disorder to gain cell entry: a hitchhiker's guide to translocation
    Daniel A Bonsor
    Department of Biology Area 10, University of York, Heslington, York, UK
    Biochem Soc Trans 36:1409-13. 2008
    ..One such group of toxins are enzymatic E colicins, protein antibiotics produced by Escherichia coli in times of stress...
  65. ncbi Hijacking cellular functions for processing and delivery of colicins E3 and D into the cytoplasm
    Miklos de Zamaroczy
    Institut de Biologie Physico Chimique, CNRS UPR 9073 associated with Université Paris 7, 75005 Paris, France
    Biochem Soc Trans 40:1486-91. 2012
    The mechanisms for importing colicins from the extracellular medium into Escherichia coli target cells implicate a complex cascade of interactions with host proteins...
  66. ncbi Involvement of OmpF during reception and translocation steps of colicin N entry
    J P Bourdineaud
    Centre de Biochimie et de Biologie Moleculaire, CNRS, Marseille, France
    Mol Microbiol 4:1737-43. 1990
    ..Colicin N does not cross-react with various monoclonal antibodies directed against colicin A...
  67. pmc A force-activated trip switch triggers rapid dissociation of a colicin from its immunity protein
    Oliver E Farrance
    School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, United Kingdom
    PLoS Biol 11:e1001489. 2013
    b>Colicins are protein antibiotics synthesised by Escherichia coli strains to target and kill related bacteria. To prevent host suicide, colicins are inactivated by binding to immunity proteins...
  68. ncbi Evolution of colicin BM plasmids: the loss of the colicin B activity gene
    Julia K Christenson
    School of Biology, Australian National University, Canberra, ACT 0200, Australia
    Microbiology 155:1645-55. 2009
    b>Colicins, a class of antimicrobial compounds produced by bacteria, are thought to be important mediators of intra- and interspecific interactions, and are a significant factor in maintaining microbial diversity...
  69. ncbi Destabilization of the colicin E9 Endonuclease domain by interaction with negatively charged phospholipids: implications for colicin translocation into bacteria
    Khedidja Mosbahi
    Department of Biology, University of York, York YO10 5YW, United Kingdom
    J Biol Chem 279:22145-51. 2004
    ..We suggest that formation of a destabilizing protein-lipid complex pre-empts channel formation by the E9 DNase and constitutes the initial step in its translocation across the Escherichia coli inner membrane...
  70. ncbi Mechanism and cleavage specificity of the H-N-H endonuclease colicin E9
    A J Pommer
    School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, UK
    J Mol Biol 314:735-49. 2001
    ..In the context of current structural information, our data show that the H-N-H motif is an adaptable catalytic centre able to hydrolyse nucleic acid by different mechanisms depending on the substrate and metal ion regime...
  71. pmc CbrA is a flavin adenine dinucleotide protein that modifies the Escherichia coli outer membrane and confers specific resistance to Colicin M
    Stephanie Helbig
    Max Planck Institute for Developmental Biology, Department of Protein Evolution, Tuebingen, Germany
    J Bacteriol 194:4894-903. 2012
    ..The next homologs with the highest identity (over 50%) are found in the anaerobic Clostridium botulinum group 1 and a few other Firmicutes...
  72. pmc Toxicity of the colicin M catalytic domain exported to the periplasm is FkpA independent
    Aurélie Barnéoud-Arnoulet
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Microbiologie de la Mediterranee, CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Bacteriol 192:5212-9. 2010
    ..Like the full-length protein, this domain is not active in the presence of the immunity protein Cmi. On the other hand, it does not require FkpA for toxic activity...
  73. ncbi Cys32 and His105 are the critical residues for the calcium-dependent cysteine proteolytic activity of CvaB, an ATP-binding cassette transporter
    Kai Hui Wu
    Department of Biology, Georgia State University, Atlanta, Georgia 30303, USA
    J Biol Chem 279:901-9. 2004
    ....
  74. pmc Probing metal ion binding and conformational properties of the colicin E9 endonuclease by electrospray ionization time-of-flight mass spectrometry
    Ewald T J van den Bremer
    Department of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands
    Protein Sci 11:1738-52. 2002
    ..In addition, our metal ion binding studies and gas-phase dissociation experiments of the ternary E9 DNase-Zn(2+)-Im9 complex have provided further evidence that electrostatic interactions govern the gas phase ion stability...
  75. ncbi Change of thermal stability of colicin E7 triggered by acidic pH suggests the existence of unfolded intermediate during the membrane-translocation phase
    K F Chak
    Institute of Biochemistry, National Yang Ming University, Taipei, Taiwan, ROC
    Proteins 32:17-25. 1998
    ..guanidine hydrochloride, indicating that this protein complex is indeed formed by aggregation of the monomeric colicins. Most interestingly, the aggregated colicins still perform in vivo bacteriocidal activity...
  76. pmc The crystal structure of the TolB box of colicin A in complex with TolB reveals important differences in the recruitment of the common TolB translocation portal used by group A colicins
    Ying Zhang
    Institute of Infection, Immunity and Inflammation, School of Molecular Medical Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, UK
    Mol Microbiol 75:623-36. 2010
    Interaction of the TolB box of Group A colicins with the TolB protein in the periplasm of Escherichia coli cells promotes transport of the cytotoxic domain of the colicin across the cell envelope...
  77. ncbi Processing of DNase domain during translocation of colicin E7 across the membrane of Escherichia coli
    C C Liao
    Institute of Biochemistry, National Yang Ming University, Shih-Pai, Taipei, Taiwan 11221, Republic of China
    Biochem Biophys Res Commun 284:556-62. 2001
    ..These results suggest that the processing of ColE7 has occurred for translocation of the DNase-type colicin across the membrane and the process is probably taking place in the periplasmic space of the membrane...
  78. ncbi Concerted folding and binding of a flexible colicin domain to its periplasmic receptor TolA
    Gregor Anderluh
    Department of Biology, Biotechnical Faculty, University of Ljubljana, Vecna pot 111, 1000 Ljubljana, Slovenia
    J Biol Chem 278:21860-8. 2003
    ..Such domains are common features of all colicins and are required for their translocation across the outer membrane of the target Escherichia coli cell...
  79. pmc Distinct conformational stability and functional activity of four highly homologous endonuclease colicins
    Ewald T J van den Bremer
    Department of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, 3584 CA Utrecht, The Netherlands e t j
    Protein Sci 13:1391-401. 2004
    ..Our results indicate that the apo-forms of these colicins are at room temperature and neutral pH in a dynamic conformational equilibrium between at least two quite ..
  80. ncbi Colicin M hydrolyses branched lipids II from Gram-positive bacteria
    Delphine Patin
    Univ Paris Sud, Laboratoire des Enveloppes Bactériennes et Antibiotiques, UMR 8619, Orsay, F 91405, France
    Biochimie 94:985-90. 2012
    ....
  81. ncbi Colicins and their potential in cancer treatment
    Lorna E Lancaster
    Institutes of Molecular Biology, University of Witten Herdecke, Stockumer Str 10, 58448 Witten, Germany
    Blood Cells Mol Dis 38:15-8. 2007
    b>Colicins are a family of antibacterial cytotoxins produced by Escherichia coli and released into the environment to reduce competition from other bacterial strains...
  82. ncbi In vitro assessment of the cytotoxicity of nisin, pediocin, and selected colicins on simian virus 40-transfected human colon and Vero monkey kidney cells with trypan blue staining viability assays
    S E Murinda
    Department of Food Science, Pennsylvania State University, University Park, Pennsylvania 16802, USA
    J Food Prot 66:847-53. 2003
    Gram-positive bacterial bacteriocins (nisin and pediocin) and gram-negative bacterial bacteriocins (colicins [Col] E1, E3, E6, E7, and K) were evaluated for cytotoxicity against cultured simian virus 40-transfected human colon (SV40-HC) ..
  83. ncbi The modes of action of colicins E5 and D, and related cytotoxic tRNases
    Haruhiko Masaki
    Department of Biotechnology, The University of Tokyo, Yayoi 1 1 1, Bunkyo ku, Tokyo 113 8657, Japan
    Biochimie 84:433-8. 2002
    b>Colicins E5 and D cleave the anticodon loops of distinct tRNAs of Escherichia coli both in vivo and in vitro, which accounts for their bactericidal actions through depletion of tRNAs and prevention of protein synthesis...
  84. pmc Inhibitory activities of colicins against Escherichia coli strains responsible for postweaning diarrhea and edema disease in swine
    Chad H Stahl
    Department of Animal Science, Iowa State University, Ames, Iowa 50011, USA
    Antimicrob Agents Chemother 48:3119-21. 2004
    The efficacies of colicins E1 and N against Escherichia coli strains responsible for postweaning diarrhea and edema disease, two of the most prevalent disease problems for pigs in the United States, were determined in vitro...
  85. ncbi Sensitivity of Shiga toxin-producing Escherichia coli (STEC) strains for colicins under different experimental conditions
    B J Jordi
    Bacteriology Division, Department of Infectious Diseases and Immunology, Utrecht University, Utrecht, The Netherlands
    FEMS Microbiol Lett 204:329-34. 2001
    Twenty Escherichia coli strains producing well-characterised colicins were tested for their inhibitory activity against five Shiga toxin-producing E. coli (STEC) strains using different media under aerobic and anaerobic conditions...
  86. pmc Translocation of inserted foreign epitopes by a channel-forming protein
    K S Jakes
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Proc Natl Acad Sci U S A 95:4321-6. 1998
    ..b>Colicins containing either epitope retain significant bactericidal activity and form channels of normal conductance in ..
  87. pmc Complete DNA sequence of a ColBM plasmid from avian pathogenic Escherichia coli suggests that it evolved from closely related ColV virulence plasmids
    Timothy J Johnson
    Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, 1802 Elwood Drive, VMRI 2, Iowa State University, Ames, IA 50011, USA
    J Bacteriol 188:5975-83. 2006
    ..These two F-type plasmids share remarkable similarity, except that they encode the production of different colicins; pAPEC-O2-ColV contains an intact ColV operon, and pAPEC-O1-ColBM encodes the colicins B and M...
  88. ncbi Following evolutionary paths to protein-protein interactions with high affinity and selectivity
    Kalia Bernath Levin
    Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, Israel
    Nat Struct Mol Biol 16:1049-55. 2009
    ....
  89. pmc Structural and functional alterations of a colicin-resistant mutant of OmpF porin from Escherichia coli
    D Jeanteur
    European Molecular Biology Laboratory, Heidelberg, Germany
    Proc Natl Acad Sci U S A 91:10675-9. 1994
    ....
  90. ncbi Lipid dependence of the channel properties of a colicin E1-lipid toroidal pore
    Alexander A Sobko
    A N Belozersky Institute of Physico Chemical Biology, Moscow State University, 119992 Moscow, Russia
    J Biol Chem 281:14408-16. 2006
    ....
  91. ncbi Importation of nuclease colicins into E coli cells: endoproteolytic cleavage and its prevention by the immunity protein
    Miklos de Zamaroczy
    Institut de Biologie Physico Chimique, CNRS, UPR 9073, 13, rue Pierre et Marie Curie 75005, Paris, France
    Biochimie 84:423-32. 2002
    A major group of colicins comprises molecules that possess nuclease activity and kill sensitive cells by cleaving RNA or DNA...
  92. pmc Colicin E3 cleavage of 16S rRNA impairs decoding and accelerates tRNA translocation on Escherichia coli ribosomes
    Lorna E Lancaster
    Institute of Molecular Biology, University of Witten Herdecke, 58448 Witten, Germany
    Mol Microbiol 69:390-401. 2008
    ..The tRNA-mRNA translocation is faster on colicin E3-cleaved than on intact ribosomes and is less sensitive to inhibition by the antibiotic viomycin...
  93. ncbi A mechanism for toxin insertion into membranes is suggested by the crystal structure of the channel-forming domain of colicin E1
    P Elkins
    Protein Engineering, Department Genentech, Inc 460 Pt San Bruno Blvd, South San Francisco, CA 94080, USA
    Structure 5:443-58. 1997
    Channel-forming colicins, including colicin E1, are a sub-family of bacteriocins...
  94. ncbi Characterization of colicinogenic Escherichia coli strains inhibitory to enterohemorrhagic Escherichia coli
    Gerry P Schamberger
    Department of Food Science and Nutrition, University of Minnesota, 1334 Eckles Avenue, St Paul, Minnesota 55108, USA
    J Food Prot 67:486-92. 2004
    ..The remaining set of 14 strains were evaluated for the presence of previously identified colicins. Seven colicins (B, El, E2/E7, E7, Ia/Ib, K, and M) were detected...
  95. pmc Competitive recruitment of the periplasmic translocation portal TolB by a natively disordered domain of colicin E9
    Steven R Loftus
    Department of Biology Area 10, University of York, York YO10 5YW, United Kingdom
    Proc Natl Acad Sci U S A 103:12353-8. 2006
    The natively disordered N-terminal 83-aa translocation (T) domain of E group nuclease colicins recruits OmpF to a colicin-receptor complex in the outer membrane (OM) as well as TolB in the periplasm of Escherichia coli, the latter ..
  96. ncbi Human tumor cells are selectively inhibited by colicins
    J Chumchalova
    Department of Biology, Faculty of Medicine, Masaryk University, 66243 Brno, Czechia
    Folia Microbiol (Praha) 48:111-5. 2003
    The activity in vitro of four types of colicins (A, E1, E3, U) against one human standard fibroblast line and against 11 human tumor-cell lines carrying defined mutations of the p53 gene was quantified by MTT (tetrazolium bromide) assay...
  97. pmc Sizing the protein translocation pathway of colicin Ia channels
    Paul K Kienker
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA
    J Gen Physiol 122:161-76. 2003
    ..Thus, the pathway for translocation is >/=26 A in diameter, or can stretch to this value. This is large enough for an alpha-helical hairpin to fit through...
  98. ncbi The colicin G, H and X determinants encode microcins M and H47, which might utilize the catecholate siderophore receptors FepA, Cir, Fiu and IroN
    S I Patzer
    Mikrobiologie Membranphysiologie, Universitat Tubingen, Auf der Morgenstelle 28, Tubingen, Germany
    Microbiology 149:2557-70. 2003
    ..These microcins could aid the producing strain in competing against enterobacteria that utilize catecholate siderophores...
  99. pmc Colicin occlusion of OmpF and TolC channels: outer membrane translocons for colicin import
    Stanislav D Zakharov
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana, USA
    Biophys J 87:3901-11. 2004
    The interaction of colicins with target cells is a paradigm for protein import. To enter cells, bactericidal colicins parasitize Escherichia coli outer membrane receptors whose physiological purpose is the import of essential metabolites...
  100. pmc TolA: a membrane protein involved in colicin uptake contains an extended helical region
    S K Levengood
    Department of Biochemistry, Duke University Medical Center, Durham, NC 27710
    Proc Natl Acad Sci U S A 88:5939-43. 1991
    The group A colicins and the DNA of many single-stranded filamentous bacteriophage are able to use combinations of the Tol proteins to gain entrance into or across the membrane of Escherichia coli...
  101. ncbi Ligand-induced changes in the conformational dynamics of a bacterial cytotoxic endonuclease
    Ewald T J van den Bremer
    Department of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands
    Biochemistry 43:4347-55. 2004
    ..In the present work we investigated the dynamic properties of the enzymatic domain of DNase colicins via time-resolved fluorescence and anisotropy decay analysis in combination with steady-state acrylamide ..

Research Grants30

  1. GATING OF PROTEIN CHANNELS IN LIPID BILAYER MEMBRANES
    Alan Finkelstein; Fiscal Year: 2013
    ..By forming chimeric channels by helix swapping between colicins Ia and A, and between diphtheria toxin's T-domain and colicin A, we shall identify which parts of colicin A ..
  2. Translocation through TonB-dependent transporters
    Karen Jakes; Fiscal Year: 2013
    ..Protein toxins called colicins that are made by some E. coli to kill other E...
  3. MOLECULAR ANALYSIS OF FERRIC ENTEROBACTIN TRANSPORT
    MARK MCINTOSH; Fiscal Year: 1993
    ....
  4. STRUCTURE OF BACTERIAL MEMBRANE PROTEINS
    R Garavito; Fiscal Year: 1992
    ..g., colicins) and in eliciting immune responses during enteric bacterial infections.
  5. FLUID BILAYER INTERACTIONS WITH PEPTIDES
    Stephen White; Fiscal Year: 2004
    ..to various subcellular compartments? How can pH-induced refolding on membranes of diphtheria toxin T-domain and colicins cause highly charged helices to cross lipid bilayers? How can short, highly basic "Trojan" peptides - such as ..
  6. MOLECULAR BASIS OF MEMBRANE EXCITATION
    Wayne Hubbell; Fiscal Year: 1999
    ..Colicin E1, a voltage-gated ion channel, will be investigated to probe molecular mechanisms of gating. ..
  7. Receptor-mediated Colicin Import
    WILLIAM CRAMER; Fiscal Year: 2007
    ..Receptor-mediated import of the toxin-like colicins is a paradigm for protein import to mitochondria and the import of viral proteins...
  8. Voltage-Gated Insertion of Colicin into Planar Bilayers
    WILLIAM CRAMER; Fiscal Year: 2005
    DESCRIPTION (provided by applicant) Studies on membrane import and channel formation of the pore-forming colicins concern: the nature of (i) the large soluble --> membrane-bound structural transition undergone by colicins, toxins, and ..
  9. EXTRA-CHROMOSOMAL GENETIC ELEMENTS AND THE CELL SURFACE
    DONNA DUCKWORTH; Fiscal Year: 1980
    ..Studies on membrane protein biosynthesis after phage infection are continuing...
  10. GENETIC ANALYSIS OF THE RECA PROTEIN OF E. COLI
    George Weinstock; Fiscal Year: 1991
    ..DNA rearrangements that result from recombination between repeated sequences will also be analyzed after DNA damaging treatments to determine if there may be constraints on recombination to maintain proper chromosome structure...
  11. BIOSYNTHESIS OF MEMBRANE PROTEINS(V2)
    Robert Webster; Fiscal Year: 2001
    ..the integrity of the outer membrane of gram negative bacteria as well as facilitating the uptake of the group A colicins. Genetics and affinity chromatography will be used to determine the cytoplasmic molecules that TolQ interacts ..
  12. ADDRESSING RESISTANCE IN ANTIMICROBIAL DESIGN
    ROBERT DORIT; Fiscal Year: 2000
    ..abstract_text> ..
  13. PROTEIN EXPORT BY AN ACCESSORY PROTEIN-SPECIFIED PATHWAY
    PHANG TAI; Fiscal Year: 1993
    ..These studies should elucidate the mechanism of CvaB-mediated Colicin V transport, and may be adapted to explore the mechanism of multi-drug resistance...
  14. STRUCTURAL BIOLOGY OF BACTERIAL COBALAMIN TRANSPORT
    Michael Wiener; Fiscal Year: 2005
    ..Also, although the natural ligands for BtuB are cobalamins, BtuB is also the receptor to which bacterial toxins (colicins) and bacteriophage bind...
  15. STRUCTURE OF THE PORE FORMING FRAGMENT OF COLICIN E1
    Cynthia Stauffacher; Fiscal Year: 1993
    ..This work will lead not only to an understanding of the structural basis for these transitions in colicin E1 but may also shed light on the general mechanism of voltage-gated channels, protein translocation, and toxin action...
  16. 600 MHZ NUCLEAR MAGNETIC RESONANCE SPECTROMETER
    LILA GIERASCH; Fiscal Year: 1999
    ..abstract_text> ..
  17. Export & Import of Lethal Agents Mediated by TolC
    Rajeev Misra; Fiscal Year: 2006
    ..Besides the obvious role of TolC in antibiotic resistance, its ability to mediate the secretion of a variety of virulence factors in pathogenic bacteria makes it a medically important subject of investigation. ..
  18. OPTICAL BIOSENSOR TO STUDY MACROMOLECULE INTERACTIONS
    WILLIAM CRAMER; Fiscal Year: 1999
    ..i) the nature of the membrane binding affinity of "membrane-active" proteins; (ii) the interaction between colicins and their cellular receptor; (iii) the binding affinity of antibodies to human rhinovirus; (iv) receptor ..
  19. Evolution of Antibiotic Resistance in Enteric Bacteria
    Margaret Riley; Fiscal Year: 2009
    ..abstract_text> ..
  20. Evolution of Stress-Induced Bacterial Toxins
    Margaret A Riley; Fiscal Year: 2010
    ....
  21. MOLECULAR EVOLUTION OF ENTERIC BACTERIOCINS
    Margaret Riley; Fiscal Year: 2002
    ..we gain regarding the natural role of bacteriocins, and the defenses raised against them within bacterial communities, can be directly applied to the rational application of novels antimicrobials to human health issues ..
  22. SIGNAL SEQUENCE RECOGNITION AND PROTEIN TARGETING
    Robert Stroud; Fiscal Year: 2007
    ..These will instruct in the mechanism of replacing the role of RNA by protein, and therefore in the essence of the dynamic roles of SRP RNA and cpSRP43. ..
  23. Molecular Basis for Transmembrane Conduction & Signaling
    Robert Stroud; Fiscal Year: 2006
    ..A further aim is to improve crystals of the Acetylcholine receptor as a means of three-dimensional structure determination of an archetype of gating mechanisms in an archetype of neuroreceptor superfamilies. ..
  24. 2005 Mechanisms of Membrane Transport Gordon Conference
    Robert Stroud; Fiscal Year: 2005
    ..abstract_text> ..
  25. GENETICS AND BIOCHEMISTRY OF RIBOSOME SYNTHESIS
    Masayasu Nomura; Fiscal Year: 2009
    ..We expect that the proposed work will make significant contributions to our understanding of transcription of rRNA genes and its regulation. ..
  26. THYMIDYLATE SYNTHASE
    Robert Stroud; Fiscal Year: 2001
    ..Irwin Kunts, UCSF. Some mechanistic question will be approached using NMR spectroscopy in collaboration with Dr. Thomas James, UCSF. ..
  27. STRUCTURE BASED DRUG DESIGN
    Robert Stroud; Fiscal Year: 2004
    ..Public domain access to the structures of therapeutic targets and technology will encourage their use by major pharmaceutical companies for the better design of therapeutics. ..