Genomes and Genes
Summary: Bacteriocins elaborated by strains of Escherichia coli and related species. They are proteins or protein-lipopolysaccharide complexes lethal to other strains of the same species.
Publications181 found, 100 shown here
- Structural and mechanistic basis of immunity toward endonuclease colicinsC Kleanthous
School of Biological Sciences, University of East Anglia, Norwich, UK
Nat Struct Biol 6:243-52. 1999..This hyperefficient mechanism of inhibition could be well suited to other toxic enzyme systems, particularly where the substrate is a polymer extending beyond the boundaries of the active site...
- Following evolutionary paths to protein-protein interactions with high affinity and selectivityKalia Bernath Levin
Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, Israel
Nat Struct Mol Biol 16:1049-55. 2009....
- The conserved asparagine in the HNH motif serves an important structural role in metal finger endonucleasesHsinchin Huang
Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, ROC
J Mol Biol 368:812-21. 2007....
- Crystal structure of colicin M, a novel phosphatase specifically imported by Escherichia coliKornelius Zeth
Department of Protein Evolution, Max Planck Institute for Developmental Biology, Spemannstrasse 35, Tubingen, Germany
J Biol Chem 283:25324-31. 2008b>Colicins are cytotoxic proteins secreted by certain strains of Escherichia coli...
- The modes of action of colicins E5 and D, and related cytotoxic tRNasesHaruhiko Masaki
Department of Biotechnology, The University of Tokyo, Yayoi 1 1 1, Bunkyo ku, Tokyo 113 8657, Japan
Biochimie 84:433-8. 2002b>Colicins E5 and D cleave the anticodon loops of distinct tRNAs of Escherichia coli both in vivo and in vitro, which accounts for their bactericidal actions through depletion of tRNAs and prevention of protein synthesis...
- Inhibitory activities of colicins against Escherichia coli strains responsible for postweaning diarrhea and edema disease in swineChad H Stahl
Department of Animal Science, Iowa State University, Ames, Iowa 50011, USA
Antimicrob Agents Chemother 48:3119-21. 2004The efficacies of colicins E1 and N against Escherichia coli strains responsible for postweaning diarrhea and edema disease, two of the most prevalent disease problems for pigs in the United States, were determined in vitro...
- Sensitivity of Shiga toxin-producing Escherichia coli (STEC) strains for colicins under different experimental conditionsB J Jordi
Bacteriology Division, Department of Infectious Diseases and Immunology, Utrecht University, Utrecht, The Netherlands
FEMS Microbiol Lett 204:329-34. 2001Twenty Escherichia coli strains producing well-characterised colicins were tested for their inhibitory activity against five Shiga toxin-producing E. coli (STEC) strains using different media under aerobic and anaerobic conditions...
- Colicins and their potential in cancer treatmentLorna E Lancaster
Institutes of Molecular Biology, University of Witten Herdecke, Stockumer Str 10, 58448 Witten, Germany
Blood Cells Mol Dis 38:15-8. 2007b>Colicins are a family of antibacterial cytotoxins produced by Escherichia coli and released into the environment to reduce competition from other bacterial strains...
- In vitro assessment of the cytotoxicity of nisin, pediocin, and selected colicins on simian virus 40-transfected human colon and Vero monkey kidney cells with trypan blue staining viability assaysS E Murinda
Department of Food Science, Pennsylvania State University, University Park, Pennsylvania 16802, USA
J Food Prot 66:847-53. 2003Gram-positive bacterial bacteriocins (nisin and pediocin) and gram-negative bacterial bacteriocins (colicins [Col] E1, E3, E6, E7, and K) were evaluated for cytotoxicity against cultured simian virus 40-transfected human colon (SV40-HC) ..
- FtsH-dependent processing of RNase colicins D and E3 means that only the cytotoxic domains are imported into the cytoplasmMathieu Chauleau
CNRS, UPR 9073, Institut de Biologie Physico Chimique, 75005 Paris, France
J Biol Chem 286:29397-407. 2011It has long been suggested that the import of nuclease colicins requires protein processing; however it had never been formally demonstrated...
- Translocation of inserted foreign epitopes by a channel-forming proteinK S Jakes
Department of Physiology and Biophysics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
Proc Natl Acad Sci U S A 95:4321-6. 1998..b>Colicins containing either epitope retain significant bactericidal activity and form channels of normal conductance in ..
- Crystal structures of the OmpF porin: function in a colicin transloconEiki Yamashita
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907 1392, USA
EMBO J 27:2171-80. 2008The OmpF porin in the Escherichia coli outer membrane (OM) is required for the cytotoxic action of group A colicins, which are proposed to insert their translocation and active domains through OmpF pores...
- Deciphering the catalytic domain of colicin M, a peptidoglycan lipid II-degrading enzymeHélène Barreteau
Universite Paris Sud 11, UMR 8619, Institut de Biochimie et Biophysique Moleculaire et Cellulaire, 91405 Orsay
J Biol Chem 285:12378-89. 2010..Most of these residues are surface-exposed and located relatively close to each other, hence suggesting they belong to the colicin M active site...
- The Tol/Pal system function requires an interaction between the C-terminal domain of TolA and the N-terminal domain of TolBAnne Walburger
Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Biologie Structurale et Microbiologie, CNRS 31, Chemin Joseph Aiguier, Marseille, France
Mol Microbiol 44:695-708. 2002..It is involved in maintaining the integrity of the outer membrane, and is required for the uptake of group A colicins and DNA of filamentous bacteriophages...
- A cka-gfp transcriptional fusion reveals that the colicin K activity gene is induced in only 3 percent of the populationJanez Mulec
Karst Research Institute, Scientific Centre of the Slovenian Academy of Sciences and Arts, Postojna, Slovenia
J Bacteriol 185:654-9. 2003..Expression of the cka-gfp fusion in a lexA-defective strain and in a relA spoT mutant strain indicates that differential expression of cka is established primarily at the level of transcription...
- The crystal structure of the nuclease domain of colicin E7 suggests a mechanism for binding to double-stranded DNA by the H-N-H endonucleasesYi Sheng Cheng
Institute of Molecular Biology, Academia Sinica, Taipei 11529, Taiwan, ROC
J Mol Biol 324:227-36. 2002..A mechanism for the binding of double-stranded DNA by dimeric H-N-H nuclease ColE7 is suggested...
- The structure of TolB, an essential component of the tol-dependent translocation system, and its protein-protein interaction with the translocation domain of colicin E9S Carr
Colicin Research Group, School of Biological Sciences, School of Chemical Sciences, University of East Anglia, Norwich, NR4 7TJ, UK
Structure 8:57-66. 2000..The translocation of enzymatic E colicins like colicin E9 is dependent upon TolB but the details of the process are not known.
- The Tol proteins of Escherichia coli and their involvement in the translocation of group A colicinsJean Claude Lazzaroni
Unité de Microbiologie et Génétique, UMR5122 CNRS INSA, Universite Lyon 1, Bâtiment André Lwoff, 10, rue Dubois, 69622 Villeurbanne Cedex, France
Biochimie 84:391-7. 2002..The Tol proteins have been parasitized by group A colicins for their translocation through the cell envelope...
- Colicin crystal structures: pathways and mechanisms for colicin insertion into membranesStanislav D Zakharov
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907-1392, USA
Biochim Biophys Acta 1565:333-46. 2002The X-ray structures of the channel-forming colicins Ia and N, and endoribonucleolytic colicin E3, as well as of the channel domains of colicins A and E1, and spectroscopic and calorimetric data for intact colicin E1, are discussed in the ..
- Local dispersal promotes biodiversity in a real-life game of rock-paper-scissorsBenjamin Kerr
Department of Biological Sciences, Stanford University, Stanford, California 94305, USA
Nature 418:171-4. 2002..We find that diversity is rapidly lost in our experimental community when dispersal and interaction occur over relatively large spatial scales, whereas all populations coexist when ecological processes are localized...
- Structural dynamics of the membrane translocation domain of colicin E9 and its interaction with TolBEmily S Collins
School of Chemical Sciences, University of East Anglia, Norwich NR4 7TJ, UK
J Mol Biol 318:787-804. 2002..The flexibility of the translocation domain of colicin E9 may be connected with its need to recognise protein partners that assist it in crossing the outer membrane and in the translocation event itself...
- Colicins produced by the Escherichia fergusonii strains closely resemble colicins encoded by Escherichia coliDavid Smajs
Department of Microbiology and Molecular Genetics, University of Texas-Houston Medical School, Houston, TX, USA
FEMS Microbiol Lett 208:259-62. 2002..fergusonii strain EF36 (pColE1-EF36) was found to be only partially immune to E1 colicins produced by two other E. fergusonii strains suggesting that pColE1-EF36 may represent an ancestor ColE1 plasmid.
- The zinc ion in the HNH motif of the endonuclease domain of colicin E7 is not required for DNA binding but is essential for DNA hydrolysisWen-Yen Ku
Graduate Institute of Life Science, National Defense Medical Center, Taipei, Taiwan 11472, Republic of China
Nucleic Acids Res 30:1670-8. 2002....
- Translocation of a functional protein by a voltage-dependent ion channelStephen L Slatin
Albert Einstein College of Medicine, Bronx, NY 10461, USA
Proc Natl Acad Sci U S A 99:1286-91. 2002..The disparate features of these proteins suggest that the colicin channel has a general protein translocation mechanism...
- The newly characterized colicin Y provides evidence of positive selection in pore-former colicin diversificationM A Riley
Department of Ecology and Evolutionary Biology, Yale University, New Haven, CT 06511, USA
Microbiology 146:1671-7. 2000..process of protein diversification of the large family of antimicrobial toxins of Escherichia coli, known as the colicins. Data from previous studies suggest that the relatively rare nuclease colicins appear to diversify primarily ..
- Ultrarapid mixing experiments reveal that Im7 folds via an on-pathway intermediateA P Capaldi
School of Biochemistry and Molecular Biology and the Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, UK
Nat Struct Biol 8:68-72. 2001..Quantitative kinetic modeling of folding and unfolding data acquired over a wide range of urea concentrations demonstrate that this intermediate ensemble lies on a direct path from the unfolded to the native state...
- Using chimeric immunity proteins to explore the energy landscape for alpha-helical protein foldingN Ferguson
School of Biochemistry and Molecular Biology, University of Leeds, Leeds LS2 9JT, UK
J Mol Biol 307:393-405. 2001....
- Cleavage of colicin D is necessary for cell killing and requires the inner membrane peptidase LepBM de Zamaroczy
Institut de Biologie Physico Chimique, CNRS, UPR 9073, 75005, Paris, France
Mol Cell 8:159-68. 2001..Catalytic colicins share a consensus sequence at their putative processing site...
- Colicin occlusion of OmpF and TolC channels: outer membrane translocons for colicin importStanislav D Zakharov
Department of Biological Sciences, Purdue University, West Lafayette, Indiana, USA
Biophys J 87:3901-11. 2004The interaction of colicins with target cells is a paradigm for protein import. To enter cells, bactericidal colicins parasitize Escherichia coli outer membrane receptors whose physiological purpose is the import of essential metabolites...
- Human tumor cells are selectively inhibited by colicinsJ Chumchalova
Department of Biology, Faculty of Medicine, Masaryk University, 66243 Brno, Czechia
Folia Microbiol (Praha) 48:111-5. 2003The activity in vitro of four types of colicins (A, E1, E3, U) against one human standard fibroblast line and against 11 human tumor-cell lines carrying defined mutations of the p53 gene was quantified by MTT (tetrazolium bromide) assay...
- Sizing the protein translocation pathway of colicin Ia channelsPaul K Kienker
Department of Physiology and Biophysics, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA
J Gen Physiol 122:161-76. 2003..Thus, the pathway for translocation is >/=26 A in diameter, or can stretch to this value. This is large enough for an alpha-helical hairpin to fit through...
- Occurrence of strains producing specific antibacterial inhibitory agents in five genera of EnterobacteriaceaeJan Smarda
Department of Biology, Faculty of Medicine, Masaryk University, Tomesova 12, 602 00, Brno, Czech Republic
Curr Microbiol 54:113-8. 2007..The siderophore activity spectrum was rather wide in any genus, but the siderophores were usually not produced by strains producing phages or colicins.
- The SOS response affects thermoregulation of colicin K synthesisMatej Butala
Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia
FEMS Microbiol Lett 283:104-11. 2008..This study investigated the effect of temperature on synthesis of Escherichia coli colicins E1, K, N and E7 as well as the molecular basis underlying thermoregulation of the colicin K activity gene cka...
- Structure of the complex of the colicin E2 R-domain and its BtuB receptor. The outer membrane colicin transloconOnkar Sharma
Department of Biological Sciences, Lilly Hall of Life Sciences, Purdue University, West Lafayette, Indiana 47907, USA
J Biol Chem 282:23163-70. 2007..It was concluded that the oblique orientation of the R-domain of the nuclease E colicins has a function in the recruitment of another member(s) of an outer membrane translocon...
- Studies on colicin B translocation: FepA is gated by TonBSurendranathan Devanathan
School of Molecular Biosciences, Washington State University, Pullman, WA 99164 4234, USA
Mol Microbiol 65:441-53. 2007..Unexpectedly, TonB was also required for colicin-induced exposure of the FepA TonB box, suggesting that TonB binds FepA at a different site prior to interaction with the TonB box...
- The role of electrostatics in colicin nuclease domain translocation into bacterial cellsDaniel Walker
Department of Biology, University of York, York YO10 5YW, United Kingdom
J Biol Chem 282:31389-97. 2007The mechanism(s) by which nuclease colicins translocate distinct cytotoxic enzymes (DNases, rRNases, and tRNases) to the cytoplasm of Escherichia coli is unknown...
- Host gastro-intestinal dynamics and the frequency of colicin production by Escherichia coliBelinda Barnes
National Centre for Epidemiology and Population Health, Australian National University, Canberra, ACT 0200, Australia
Microbiology 153:2823-7. 2007The production of antimicrobial compounds known as colicins has been shown to be an important mediator of competitive interactions among Escherichia coli genotypes...
- Anomalous proton selectivity in a large channel: colicin AStephen L Slatin
Department of Physiology and Biophysics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
Biochemistry 47:1778-88. 2008Some of the bactericidal proteins known as colicins exert their toxic action by forming a large, nonselective channel in the inner membrane of target bacteria. The structure of this channel is unknown...
- The role of SOS boxes in enteric bacteriocin regulationOsnat Gillor
Department of Environmental Hydrology and Microbiology, Zuckerberg Institute for Water Research, J Blaustein Institutes for Desert Research, Ben Gurion University of the Negev, Sede Boqer Campus, 84990 Midreshet Ben Gurion, Israel
Microbiology 154:1783-92. 2008Bacteriocins are a large and functionally diverse family of toxins found in all major lineages of Bacteria. Colicins, those bacteriocins produced by Escherichia coli, serve as a model system for investigations of bacteriocin structure-..
- The colicin E3 outer membrane translocon: immunity protein release allows interaction of the cytotoxic domain with OmpF porinStanislav D Zakharov
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
Biochemistry 45:10199-207. 2006....
- Competitive recruitment of the periplasmic translocation portal TolB by a natively disordered domain of colicin E9Steven R Loftus
Department of Biology Area 10, University of York, York YO10 5YW, United Kingdom
Proc Natl Acad Sci U S A 103:12353-8. 2006The natively disordered N-terminal 83-aa translocation (T) domain of E group nuclease colicins recruits OmpF to a colicin-receptor complex in the outer membrane (OM) as well as TolB in the periplasm of Escherichia coli, the latter ..
- The colicin G, H and X determinants encode microcins M and H47, which might utilize the catecholate siderophore receptors FepA, Cir, Fiu and IroNS I Patzer
Mikrobiologie Membranphysiologie, Universitat Tubingen, Auf der Morgenstelle 28, Tubingen, Germany
Microbiology 149:2557-70. 2003..These microcins could aid the producing strain in competing against enterobacteria that utilize catecholate siderophores...
- Ligand-induced changes in the conformational dynamics of a bacterial cytotoxic endonucleaseEwald T J van den Bremer
Department of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands
Biochemistry 43:4347-55. 2004..In the present work we investigated the dynamic properties of the enzymatic domain of DNase colicins via time-resolved fluorescence and anisotropy decay analysis in combination with steady-state acrylamide ..
- Ultraviolet resonance Raman studies reveal the environment of tryptophan and tyrosine residues in the native and partially folded states of the E colicin-binding immunity protein Im7Iñigo R Rodriguez-Mendieta
Institute of Molecular Biophysics and Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, United Kingdom
Biochemistry 44:3306-15. 2005..We corroborated this observation using tryptophan-containing model compounds and conclude that the conventional interpretation of this UVRR feature at these wavelengths is unreliable...
- Identification of an essential cleavage site in ColE7 required for import and killing of cellsZhonghao Shi
Institute of Biochemistry, National Yang-Ming University, Taipei 11221, Taiwan and Institute of Molecular Biology, Academia Sinica, Taipei 11529, Taiwan
J Biol Chem 280:24663-8. 2005..Nuclease colicins are processed during translocation with only the cytotoxic nuclease domains traversing the inner membrane to ..
- Clusters in an intrinsically disordered protein create a protein-binding site: the TolB-binding region of colicin E9Kaeko Tozawa
School of Chemical Sciences and Pharmacy, University of East Anglia, Norwich NR4 7TJ, United Kingdom
Biochemistry 44:11496-507. 2005....
- Crystal structural analysis and metal-dependent stability and activity studies of the ColE7 endonuclease domain in complex with DNA/Zn2+ or inhibitor/Ni2+Lyudmila G Doudeva
Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan 11529, Republic of China
Protein Sci 15:269-80. 2006....
- Calorimetric dissection of colicin DNase--immunity protein complex specificityAnthony H Keeble
Department of Biology (Area 10, Post Office Box 373, University of York, Heslington, York YO10 5YW, United Kingdom
Biochemistry 45:3243-54. 2006..capacity (DeltaC(p)) accompanying binding of each Im protein (Im2, Im7, Im8, and Im9) to the DNase domains of colicins E2, E7, E8, and E9, in the context of both cognate and noncognate complexes...
- Exoproducts of the Escherichia coli strain H22 inhibiting some enteric pathogens both in vitro and in vivoL Cursino
Department of General Biology, ICB Universidade Federal de Minas Gerais, Belo Horizonte MG, Brazil
J Appl Microbiol 100:821-9. 2006..The antagonistic activity of the Escherichia coli strain H22 against enteric bacteria was studied both in vitro and in vivo...
- Hot-spot residues at the E9/Im9 interface help binding via different mechanismsSergio E Wong
Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, CA 92093 0365, USA
Biopolymers 89:916-20. 2008..This type of model may be helpful in engineering hot-spot clusters at protein-protein interfaces and, consequently, the design of specificity...
- Role of TolR N-terminal, central, and C-terminal domains in dimerization and interaction with TolA and tolQL Journet
Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Biologie Structurale et Microbiologie, Centre National de la Recherche Scientifique, 13402 Marseille Cedex 20, France
J Bacteriol 181:4476-84. 1999..a multiprotein system involved in maintaining the cell envelope integrity and is necessary for the import of some colicins and phage DNA into the bacterium...
- Structure of the membrane-pore-forming fragment of colicin AM W Parker
European Molecular Biology Laboratory, Heidelberg, FRG
Nature 337:93-6. 1989b>Colicins are antibiotic proteins produced by and active against sensitive Escherichia coli and closely related bacteria...
- Colicin cleavage by OmpT protease during both entry into and release from Escherichia coli cellsD Cavard
Centre de Biochimie et de Biologie Moleculaire, Centre National de la Recherche Scientifique, Marseille, France
J Bacteriol 172:648-52. 1990Proteolysis of colicins A, E1, E2, and E3 was observed after they were added to whole cells carrying a functional ompT gene. Recombinant plasmid pML19 containing the ompT gene enabled two mutant strains to cleave the added colicins...
- TolA: a membrane protein involved in colicin uptake contains an extended helical regionS K Levengood
Department of Biochemistry, Duke University Medical Center, Durham, NC 27710
Proc Natl Acad Sci U S A 88:5939-43. 1991The group A colicins and the DNA of many single-stranded filamentous bacteriophage are able to use combinations of the Tol proteins to gain entrance into or across the membrane of Escherichia coli...
- Evolutionary relationship of uptake systems for biopolymers in Escherichia coli: cross-complementation between the TonB-ExbB-ExbD and the TolA-TolQ-TolR proteinsV Braun
Mikrobiologie II, Universitat Tubingen, Germany
Mol Microbiol 8:261-8. 1993..Group B colicins, ferric siderophores and vitamin B12 are taken up via the TonB-ExbB-ExbD, group A colicins via the TolA-TolQ-TolR ..
- Mutational analyses define helix organization and key residues of a bacterial membrane energy-transducing complexEmilie L Goemaere
Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Biologie Structurale et Microbiologie, CNRS UPR 9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
J Mol Biol 366:1424-36. 2007..The Tol system is parasitized by bacterial toxins called colicins, which are imported through the outer membrane using an energy-independent process...
- Nucleotide sequences of the tolA and tolB genes and localization of their products, components of a multistep translocation system in Escherichia coliS K Levengood
Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710
J Bacteriol 171:6600-9. 1989..tolQRAB gene cluster of Escherichia coli render the bacteria tolerant to high concentrations of the E, A, or K colicins as well as tolerant to infection by the single-stranded filamentous bacteriophage. The nucleotide sequence of a 2...
- DNA and amino acid sequence analysis of structural and immunity genes of colicins Ia and IbJ A Mankovich
J Bacteriol 168:228-36. 1986The nucleotide sequences for colicin Ia and colicin Ib structural and immunity genes were determined. The two colicins each consist of 626 amino acid residues...
- A novel transposon-like structure carries the genes for pyocin AP41, a Pseudomonas aeruginosa bacteriocin with a DNase domain homology to E2 group colicinsY Sano
Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan
Mol Gen Genet 237:161-70. 1993..component (the deoxyribonuclease domain) and the immunity protein show remarkable homology to those of E2 group colicins, suggesting that these bacteriocins, which are produced by distantly related species, have originated from a ..
- Ion-channel-forming colicinsR M Stroud
Department of Biochemistry and Biophysics, University of California, San Francisco School of Medicine 94143 0448, USA
Curr Opin Struct Biol 8:525-33. 1998Several features of ion-channel-forming colicins have been illuminated by recent revelations: its four-domain structure, the mechanism and thermodynamics of binding to the gating loop of outer membrane porins, the mechanism of ..
- Characterization of colicin S4 and its receptor, OmpW, a minor protein of the Escherichia coli outer membraneH Pilsl
Mikrobiologie Membranphysiologie, Universitat Tubingen, Tubingen, Germany
J Bacteriol 181:3578-81. 1999..By contrast, the predicted receptor binding domain shows no sequence similarities to other colicins. Mutants that lacked the OmpW protein were resistant to colicin S4.
- Immunity proteins and their specificity for endonuclease colicins: telling right from wrong in protein-protein recognitionC Kleanthous
School of Biological Sciences, University of East Anglia, Norwich, UK
Mol Microbiol 28:227-33. 1998Immunity proteins inhibit colicins, protein toxins released by bacteria during times of environmental stress, by binding and inactivating their cytotoxic domains...
- Colicin biologyEric Cascales
Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Biologie Structurale et Microbiologie, Centre National de la Recherche Scientifique, UPR 9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
Microbiol Mol Biol Rev 71:158-229. 2007b>Colicins are proteins produced by and toxic for some strains of Escherichia coli. They are produced by strains of E. coli carrying a colicinogenic plasmid that bears the genetic determinants for colicin synthesis, immunity, and release...
- Structural inhibition of the colicin D tRNase by the tRNA-mimicking immunity proteinMarc Graille
LEBS, CNRS, UPR 9063, Gif sur Yvette, France
EMBO J 23:1474-82. 2004b>Colicins are toxins secreted by Escherichia coli in order to kill their competitors...
- Structure-function analysis of the vitamin B12 receptor of Escherichia coli by means of informational suppressionS E Hufton
Department of Biochemistry, University of Nottingham Medical School, Queen s Medical Centre, UK
Mol Microbiol 15:381-93. 1995..Most amino acid substitutions have a pleiotropic effect with respect to all ligands tested, the two colicins E1 and E3, the T5-like bacteriophage BF23, and vitamin B12...
- Tol-dependent macromolecule import through the Escherichia coli cell envelope requires the presence of an exposed TolA binding motifStéphanie Pommier
Institut de Biologie Structurale et de Microbiologie, CNRS, UPR 9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
J Bacteriol 187:7526-34. 2005..The Tol proteins are parasitized by filamentous bacteriophages and group A colicins. The N-terminal domain of the Ff phage g3p protein and the translocation domains of colicins interact directly ..
- Enzymatic E-colicins bind to their target receptor BtuB by presentation of a small binding epitope on a coiled-coil scaffoldArun K Mohanty
Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia 22908 0736, USA
J Biol Chem 278:40953-8. 2003..Bacterial E-colicins target Escherichia coli by binding to the outer membrane cobalamin transporter BtuB...
- Involvement of ArgR and PepA in the pairing of ColE1 dimer resolution sitesA Guhathakurta
Microbiology 141:1163-71. 1995..The validity of the pairing hypothesis is discussed in the light of alternative explanations for our data...
- Deletion and substitution analysis of the Escherichia coli TonB Q160 regionHema Vakharia-Rao
School of Molecular Biosciences, Washington State University, Pullman, WA 99164, USA
J Bacteriol 189:4662-70. 2007..It is hypothesized that the TonB Q160 region may be part of a large disordered region required to span the periplasm and contact an OM transporter...
- Concerted folding and binding of a flexible colicin domain to its periplasmic receptor TolAGregor Anderluh
Department of Biology, Biotechnical Faculty, University of Ljubljana, Vecna pot 111, 1000 Ljubljana, Slovenia
J Biol Chem 278:21860-8. 2003..Such domains are common features of all colicins and are required for their translocation across the outer membrane of the target Escherichia coli cell...
- Structural dynamics of the receptor-binding domain of colicin E9Ruth Boetzel
School of Chemical Sciences, University of East Anglia, Norwich NR4 7TJ, UK
Faraday Discuss 122:145-62; discussion 171-90. 2003..A possible biological role for this involves the hairpin opening during translocation into bacteria...
- Activity domains of the TonB proteinI Traub
Mikrobiologie II, Universitat Tubingen, Germany
Mol Microbiol 8:409-23. 1993..Uptake of colicins required different domains in TonB, for colicin B and M around residue 160 and for colicin Ia, a domain closer to ..
- Evidence that the immunity protein inactivates colicin 5 immediately prior to the formation of the transmembrane channelH Pilsl
Universitat Tubingen, Germany
J Bacteriol 177:6966-72. 1995..of the activity, immunity, and lysis genes of colicin 5 assigned colicin 5 to the subclass of pore-forming colicins to which colicins 10, E1, Ia, Ib, and K belong...
- Flexibility in the receptor-binding domain of the enzymatic colicin E9 is required for toxicity against Escherichia coli cellsChristopher N Penfold
School of Molecular Medical Sciences and Institute of Infection, Immunity and Inflammation, Centre for Biomolecular Sciences, University of Nottingham, Nottingham NG7 2RD, United Kingdom
J Bacteriol 186:4520-7. 2004The events that occur after the binding of the enzymatic E colicins to Escherichia coli BtuB receptors that lead to translocation of the cytotoxic domain into the periplasmic space and, ultimately, cell killing are poorly understood...
- Loop deletions indicate regions important for FhuA transport and receptor functions in Escherichia coliFranziska Endriss
Mikrobiologie Membranphysiologie, Universitat Tubingen, Tubingen, Germany
J Bacteriol 186:4818-23. 2004..Presumably, FhuA evolved as a ferrichrome transporter and was subsequently used as a receptor by the phages and colicin M, which selected the same as well as distinct loops as receptor sites...
- Interactions of TolB with the translocation domain of colicin E9 require an extended TolB boxSarah L Hands
Centre for Biomolecular Sciences, University Park, University of Nottingham, Nottingham NG7 2RD, United Kingdom
J Bacteriol 187:6733-41. 2005The mechanism by which enzymatic E colicins such as colicin E3 (ColE3) and ColE9 cross the outer membrane, periplasm, and cytoplasmic membrane to reach the cytoplasm and thus kill Escherichia coli cells is unique in prokaryotic biology ..
- Cell entry mechanism of enzymatic bacterial colicins: porin recruitment and the thermodynamics of receptor bindingNicholas G Housden
Department of Biology Area 10, P O Box 373, University of York, York YO10 5YW, United Kingdom
Proc Natl Acad Sci U S A 102:13849-54. 2005Binding of enzymatic E colicins to the vitamin B12 receptor, BtuB, is the first stage in a cascade of events that culminate in the translocation of the cytotoxic nuclease into the Escherichia coli cytoplasm and release of its tightly ..
- High-resolution crystal structure of a truncated ColE7 translocation domain: implications for colicin transport across membranesYi-Sheng Cheng
Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, ROC
J Mol Biol 356:22-31. 2006..ColE7 is classified as one of the group A colicins, since the N-terminal translocation domain (T-domain) of the nuclease-type colicins interact with specific ..
- Cloning and promoter identification of the iron-regulated cir gene of Escherichia coliD W Griggs
Department of Microbiology, University of Illinois, Urbana 61801
J Bacteriol 169:5343-52. 1987....
- Topology analysis of the colicin V export protein CvaA in Escherichia coliR C Skvirsky
Department of Biology, University of Massachusetts Boston 02125, USA
J Bacteriol 177:6153-9. 1995..This study demonstrates the application of AP fusion analysis to elucidate the topology of a membrane-associated protein having only a single transmembrane domain...
- Multiple extracellular loops contribute to substrate binding and transport by the Escherichia coli cobalamin transporter BtuBCynthia A Fuller-Schaefer
Department of Microbiology, University of Virginia, Charlottesville, VA 22908 0734, USA
J Bacteriol 187:1732-9. 2005..These phenotypes show that there are multiple independent binding elements and point out similarities and differences in binding properties among the TonB-dependent transporters...
- Molecular basis of inhibition of the ribonuclease activity in colicin E5 by its cognate immunity proteinCesar Luna-Chavez
Center for Biophysics and Computational Biology, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
J Mol Biol 358:571-9. 2006....
- Role of Cal, the colicin A lysis protein, in two steps of colicin A release and in the interaction with colicin A-porin complexesDaniele Cavard
Laboratoire d Ingenierie des Systemes Macromoleculaires, Centre National de la Recherche Scientifique, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
Microbiology 150:3867-75. 2004..of the pools of released and unreleased colicin A together with the hetero-oligomers of colicin A and porins (colicins Au)...
- Release of immunity protein requires functional endonuclease colicin import machineryDenis Duché
Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Biologie Structurale et Microbiologie, CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
J Bacteriol 188:8593-600. 2006Bacteria producing endonuclease colicins are protected against the cytotoxic activity by a small immunity protein that binds with high affinity and specificity to inactivate the endonuclease...
- Crystal structure of colicin E3: implications for cell entry and ribosome inactivationS Soelaiman
Department of Biochemistry, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA
Mol Cell 8:1053-62. 2001b>Colicins kill E. coli by a process that involves binding to a surface receptor, entering the cell, and, finally, intoxicating it. The lethal action of colicin E3 is a specific cleavage in the ribosomal decoding A site...
- Characterisation of a mobile protein-binding epitope in the translocation domain of colicin E9Colin J Macdonald
School of Chemical Sciences and Pharmacy, University of East Anglia, Norwich NR4 7TJ, UK
J Biomol NMR 30:81-96. 2004....
- Initial steps of colicin E1 import across the outer membrane of Escherichia coliMuriel Masi
School of Life Sciences, Arizona State University, Tempe, AZ 85287 4501, USA
J Bacteriol 189:2667-76. 2007..coli cells from infection by E colicins. A genetic strategy for isolating tolC mutations that confer resistance to ColE1, without affecting other TolC ..
- A sequence-specific RNase activity derived from the interface of the dimeric immunity protein of the ColE7 operonChen Chung Liao
Institute of Biochemistry, National Yang Ming University, Taipei 11221 Taiwan
Protein Pept Lett 14:147-50. 2007....
- Investigating early events in receptor binding and translocation of colicin E9 using synchronized cell killing and proteolytic cleavageYing Zhang
School of Molecular Medical Sciences, Institute of Infection, Immunity and Inflammation, Centre for Biomolecular Sciences, University Park, Nottingham NG7 2RD, United Kingdom
J Bacteriol 190:4342-50. 2008Enzymatic colicins such as colicin E9 (ColE9) bind to BtuB on the cell surface of Escherichia coli and rapidly recruit a second coreceptor, either OmpF or OmpC, through which the N-terminal natively disordered region (NDR) of their ..
- Evolutionary relationship between the TonB-dependent outer membrane transport proteins: nucleotide and amino acid sequences of the Escherichia coli colicin I receptor geneC D Nau
Department of Microbiology, University of Illinois, Urbana 61801
J Bacteriol 171:1041-7. 1989..On the basis of the multiple progressive sequence alignment and the similarity scores derived from it, a tree representing evolutionary distance between five TonB-dependent outer membrane transport proteins was generated...
- On the interaction of colicin E3 with the ribosomeRaz Zarivach
Weizmann Institute of Science, Department of Structural Biology, Rehovot 76100, Israel
Biochimie 84:447-54. 2002..Furthermore, the model structure suggests that the inhibitory action of bound immunity is due to charge repulsion of this acidic protein by the negatively charged rRNA backbone..
- The nucleoside-specific Tsx channel from the outer membrane of Salmonella typhimurium, Klebsiella pneumoniae and Enterobacter aerogenes: functional characterization and DNA sequence analysis of the tsx genesA Nieweg
Department of Biology, University of Konstanz, Germany
Microbiology 143:603-15. 1997..channel for deoxynucleosides and the antibiotic albicidin, and also serves as a receptor for bacteriophages and colicins. We cloned the structural genes of the Tsx proteins from Salmonella typhimurium, Klebsiella pneumoniae and ..
- The TolA protein interacts with colicin E1 differently than with other group A colicinsS L Schendel
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907 1392, USA
J Bacteriol 179:3683-90. 1997The 421-residue protein TolA is required for the translocation of group A colicins (colicins E1, E2, E3, A, K, and N) across the cell envelope of Escherichia coli...
- Macromolecular import into Escherichia coli: the TolA C-terminal domain changes conformation when interacting with the colicin A toxinChristophe Deprez
Institut de Biologie Structurale Jean Pierre Ebel, CEA CNRS UJF, UMR 5075, 41 rue Jules Horowitz, 38027 Grenoble Cedex 1, France
Biochemistry 41:2589-98. 2002..It was shown that the TolA, TolQ, and TolR proteins play a critical role in the translocation of group A colicins and filamentous bacteriophage minor coat proteins (g3p)...
- Import of colicins across the outer membrane of Escherichia coli involves multiple protein interactions in the periplasmL Journet
CNRS, LISM, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
Mol Microbiol 42:331-44. 2001..This interaction is specific to colicins dependent on TolR for their translocation, strongly suggesting a direct involvement of the interaction in the ..
- In vivo synthesis of the periplasmic domain of TonB inhibits transport through the FecA and FhuA iron siderophore transporters of Escherichia coliS P Howard
Mikrobiologie II, Universitat Tubingen, D 72076 Tubingen, Germany
J Bacteriol 183:5885-95. 2001....
- Mutations in the Escherichia coli receptor FepA reveal residues involved in ligand binding and transportT J Barnard
Department of Molecular Microbiology and Immunology, University of Missouri School of Medicine, Columbia, Missouri 65212, USA
Mol Microbiol 41:527-36. 2001..A third region formed a cluster near the barrel wall around positions 75 and 126 of the plug. These mutations provide initial insight into the mechanisms of ligand binding and transport through the FepA receptor...
- Functional analysis of a C-terminally altered TonB protein of Escherichia coliM Anton
Fakultat fur Biologie, Universitat Konstanz, F R G
Gene 105:23-9. 1991..The possibility is discussed that the C-terminal hydrophobic region is involved in the intramolecular interaction rather than in the membrane association of TonB...
- The TolC protein of Escherichia coli serves as a cell-surface receptor for the newly characterized TLS bacteriophageG J German
Department of Microbiology, Arizona State University, Tempe, AZ 85287, USA
J Mol Biol 308:579-85. 2001....
- The structure, function, and origin of the microcin H47 ATP-binding cassette exporter indicate its relatedness to that of colicin VM F Azpiroz
Sección de Fisiología y Genética Bacterianas, Facultad de Ciencias, Montevideo 11 400, Uruguay
Antimicrob Agents Chemother 45:969-72. 2001..The results of sequence and gene fusion analyses, as well as heterologous complementation assays, are presented...
- Enzymological characterization of the nuclease domain from the bacterial toxin colicin E9 from Escherichia coliA J Pommer
School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
Biochem J 334:387-92. 1998....
- GATING OF PROTEIN CHANNELS IN LIPID BILAYER MEMBRANESAlan Finkelstein; Fiscal Year: 1993..into membranes by bacterial proteins such as diphtheria toxin, tetanus toxin, botulinum toxin, anthrax toxin, and colicins of the E1 class (E1, Ia, Ib and A)...
- GATING OF PROTEIN CHANNELS IN LIPID BILAYER MEMBRANESAlan Finkelstein; Fiscal Year: 2010..By forming chimeric channels by helix swapping between colicins Ia and A, and between diphtheria toxin's T-domain and colicin A, we shall identify which parts of colicin A ..
- DOMAIN MAPPING CLOSTRIDIUM PERFRINGENS THETA TOXINRodney Tweten; Fiscal Year: 2000..Unlike many bacterial toxins, such as diphtheria toxin or the colicins, pH does not act as a trigger for the formation of a "molten globule" or insertion intermediate...
- GATING OF PROTEIN CHANNELS IN LIPID BILAYER MEMBRANESAlan Finkelstein; Fiscal Year: 1990..those inserted into membranes by bacterial proteins, such as diptheria toxin, tetanus toxin, botulinum toxin, and colicins of the E1 class (E1, Ib, A and K), and those normally present in plasma membranes, such as the voltage-dependent ..
- LOOP DELETION MUTAGENESIS OF FEPAPHILLIP KLEBBA; Fiscal Year: 1999..3. Conformation: binding of antibodies and colicins to surface loops, and maintenance of trimeric structure. 4...
- MOLECULAR MECHANISMS OF MEMBRANE TRANSPORTDavid Cafiso; Fiscal Year: 2003..to the accumulation of nutrients, this class of proteins functions in the uptake of bactericidal agents, such as colicins, phages and small molecule antibiotics...
- STRUCTURE/FUNCTION OF MEMBRANE PROTEINSWilliam Dowhan; Fiscal Year: 2000..phospholipids have been implicated in protein translocation across the cytoplasmic membrane, the insertion of colicins into membranes, and the initiation of DNA synthhesis...
- STRUCTURE AND FUNCTION OF MEMBRANE PROTEINSWilliam Dowhan; Fiscal Year: 1993..phospholipids have been implicated in protein translocation across the cytoplasmic membrane, the insertion of colicins into membranes, and the initiation of DNA synthhesis...
- FLUID BILAYER INTERACTIONS WITH PEPTIDESStephen White; Fiscal Year: 2004..to various subcellular compartments? How can pH-induced refolding on membranes of diphtheria toxin T-domain and colicins cause highly charged helices to cross lipid bilayers? How can short, highly basic "Trojan" peptides - such as ..
- STRUCTURE & FUNCTION OF THE COLICIN E1 CHANNELWILLIAM CRAMER; Fiscal Year: 1993....
- Translocation through TonB-dependent transportersKaren Jakes; Fiscal Year: 2011..Protein toxins called colicins that are made by some E. coli to kill other E...
- STRUCTURE/FUNCTION OF COLICIN E1/RECEPTOR/IMM PROTEINWILLIAM CRAMER; Fiscal Year: 2002..A weak interaction precludes effective binding. One that is too strong prevents insertion into the bilayer. ..
- BIOSYNTHESIS OF MEMBRANE PROTEINSRobert Webster; Fiscal Year: 1993..role of the TolQRA and B proteins in maintaining membrane integrity and facilitating the uptake of the group A colicins will be investigated...
- THE MECHANISM OF ACTION OF COLICINWILLIAM CRAMER; Fiscal Year: 1980..The latter studies will be complemented by using a small 20 k dalton tryptic fragment of colicin E1 which is active in the synthetic lipid vesicles. ..
- TRANSPORT OF VITAMIN B12 IN E COLIROBERT KADNER; Fiscal Year: 1993..The relation of this process to the uptake of colicins, which utilize the B12 receptor will be explored, initially by examination of the requirement for btuC function ..
- Receptor-mediated Colicin ImportWILLIAM CRAMER; Fiscal Year: 2007..Receptor-mediated import of the toxin-like colicins is a paradigm for protein import to mitochondria and the import of viral proteins...