colicins

Summary

Summary: Bacteriocins elaborated by strains of Escherichia coli and related species. They are proteins or protein-lipopolysaccharide complexes lethal to other strains of the same species.

Top Publications

  1. ncbi Bacteriocins: evolution, ecology, and application
    Margaret A Riley
    Department of Ecology and Evolutionary Biology, Yale University, New Haven, Connecticut 06511, USA
    Annu Rev Microbiol 56:117-37. 2002
  2. doi Swimming against the tide: progress and challenges in our understanding of colicin translocation
    Colin Kleanthous
    Department of Biology, University of York, Wentworth Way, Heslington, YO10 5DD, UK
    Nat Rev Microbiol 8:843-8. 2010
  3. pmc Allosteric beta-propeller signalling in TolB and its manipulation by translocating colicins
    Daniel A Bonsor
    Department of Biology, University of York, York, UK
    EMBO J 28:2846-57. 2009
  4. pmc Colicin biology
    Eric Cascales
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Biologie Structurale et Microbiologie, Centre National de la Recherche Scientifique, UPR 9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Microbiol Mol Biol Rev 71:158-229. 2007
  5. pmc Directed epitope delivery across the Escherichia coli outer membrane through the porin OmpF
    Nicholas G Housden
    Department of Biology, Area 10, and York Structural Biology Laboratory, University of York, Heslington, York YO10 5DD, United Kingdom
    Proc Natl Acad Sci U S A 107:21412-7. 2010
  6. pmc Mobility of BtuB and OmpF in the Escherichia coli outer membrane: implications for dynamic formation of a translocon complex
    Jeff Spector
    Department of Physics, Purdue University, West Lafayette, Indiana, USA
    Biophys J 99:3880-6. 2010
  7. pmc FtsH-dependent processing of RNase colicins D and E3 means that only the cytotoxic domains are imported into the cytoplasm
    Mathieu Chauleau
    CNRS, UPR 9073, Institut de Biologie Physico Chimique, 75005 Paris, France
    J Biol Chem 286:29397-407. 2011
  8. doi Nuclease colicins and their immunity proteins
    Grigorios Papadakos
    Department of Biology, University of York, Wentworth Way, Heslington, York YO10 5DD, UK
    Q Rev Biophys 45:57-103. 2012
  9. pmc Low resolution structure and dynamics of a colicin-receptor complex determined by neutron scattering
    Luke A Clifton
    ISIS Spallation Neutron Source, Rutherford Appleton Laboratory, Harwell Science and Innovation Campus, Didcot, Oxfordshire OX11 0QX, United Kingdom
    J Biol Chem 287:337-46. 2012
  10. pmc Human- and plant-pathogenic Pseudomonas species produce bacteriocins exhibiting colicin M-like hydrolase activity towards peptidoglycan precursors
    Hélène Barreteau
    Universite Paris Sud, Institut de Biochimie et Biophysique Moleculaire et Cellulaire, UMR 8619, Orsay F 91405, France
    J Bacteriol 191:3657-64. 2009

Research Grants

  1. GATING OF PROTEIN CHANNELS IN LIPID BILAYER MEMBRANES
    Alan Finkelstein; Fiscal Year: 1993
  2. GATING OF PROTEIN CHANNELS IN LIPID BILAYER MEMBRANES
    Alan Finkelstein; Fiscal Year: 2010
  3. DOMAIN MAPPING CLOSTRIDIUM PERFRINGENS THETA TOXIN
    Rodney Tweten; Fiscal Year: 2000
  4. GATING OF PROTEIN CHANNELS IN LIPID BILAYER MEMBRANES
    Alan Finkelstein; Fiscal Year: 1990
  5. LOOP DELETION MUTAGENESIS OF FEPA
    PHILLIP KLEBBA; Fiscal Year: 1999
  6. MOLECULAR MECHANISMS OF MEMBRANE TRANSPORT
    David Cafiso; Fiscal Year: 2003
  7. STRUCTURE/FUNCTION OF MEMBRANE PROTEINS
    William Dowhan; Fiscal Year: 2000
  8. STRUCTURE AND FUNCTION OF MEMBRANE PROTEINS
    William Dowhan; Fiscal Year: 1993
  9. FLUID BILAYER INTERACTIONS WITH PEPTIDES
    Stephen White; Fiscal Year: 2004
  10. STRUCTURE & FUNCTION OF THE COLICIN E1 CHANNEL
    WILLIAM CRAMER; Fiscal Year: 1993

Detail Information

Publications216 found, 100 shown here

  1. ncbi Bacteriocins: evolution, ecology, and application
    Margaret A Riley
    Department of Ecology and Evolutionary Biology, Yale University, New Haven, Connecticut 06511, USA
    Annu Rev Microbiol 56:117-37. 2002
    ..In the latter half of this review we focus on the potential role bacteriocins may play in addressing human health concerns and the current role they serve in food preservation...
  2. doi Swimming against the tide: progress and challenges in our understanding of colicin translocation
    Colin Kleanthous
    Department of Biology, University of York, Wentworth Way, Heslington, YO10 5DD, UK
    Nat Rev Microbiol 8:843-8. 2010
    b>Colicins are folded protein toxins that face the formidable task of translocating across one or both of the Escherichia coli cell membranes in order to induce cell death. This translocation is achieved by parasitizing host proteins...
  3. pmc Allosteric beta-propeller signalling in TolB and its manipulation by translocating colicins
    Daniel A Bonsor
    Department of Biology, University of York, York, UK
    EMBO J 28:2846-57. 2009
    The Tol system is a five-protein assembly parasitized by colicins and bacteriophages that helps stabilize the Gram-negative outer membrane (OM)...
  4. pmc Colicin biology
    Eric Cascales
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Biologie Structurale et Microbiologie, Centre National de la Recherche Scientifique, UPR 9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Microbiol Mol Biol Rev 71:158-229. 2007
    b>Colicins are proteins produced by and toxic for some strains of Escherichia coli. They are produced by strains of E. coli carrying a colicinogenic plasmid that bears the genetic determinants for colicin synthesis, immunity, and release...
  5. pmc Directed epitope delivery across the Escherichia coli outer membrane through the porin OmpF
    Nicholas G Housden
    Department of Biology, Area 10, and York Structural Biology Laboratory, University of York, Heslington, York YO10 5DD, United Kingdom
    Proc Natl Acad Sci U S A 107:21412-7. 2010
    ..Our study explains how colicins exploit porins to deliver epitope signals to the bacterial periplasm and, more broadly, how the inherent ..
  6. pmc Mobility of BtuB and OmpF in the Escherichia coli outer membrane: implications for dynamic formation of a translocon complex
    Jeff Spector
    Department of Physics, Purdue University, West Lafayette, Indiana, USA
    Biophys J 99:3880-6. 2010
    ..vitamin B12) receptor (BtuB) and the OmpF porin, which are implicated in the cellular import pathways of colicins and phages-was measured in vivo...
  7. pmc FtsH-dependent processing of RNase colicins D and E3 means that only the cytotoxic domains are imported into the cytoplasm
    Mathieu Chauleau
    CNRS, UPR 9073, Institut de Biologie Physico Chimique, 75005 Paris, France
    J Biol Chem 286:29397-407. 2011
    It has long been suggested that the import of nuclease colicins requires protein processing; however it had never been formally demonstrated...
  8. doi Nuclease colicins and their immunity proteins
    Grigorios Papadakos
    Department of Biology, University of York, Wentworth Way, Heslington, York YO10 5DD, UK
    Q Rev Biophys 45:57-103. 2012
    ..coli, known collectively as nuclease colicins, and the specific immunity (inhibitor) proteins that colicin-producing organisms make in order to avoid ..
  9. pmc Low resolution structure and dynamics of a colicin-receptor complex determined by neutron scattering
    Luke A Clifton
    ISIS Spallation Neutron Source, Rutherford Appleton Laboratory, Harwell Science and Innovation Campus, Didcot, Oxfordshire OX11 0QX, United Kingdom
    J Biol Chem 287:337-46. 2012
    ..This revealed that colicin N unfolds and binds to the OmpF-lipid interface. The implications of this unfolding step for colicin translocation across membranes are discussed...
  10. pmc Human- and plant-pathogenic Pseudomonas species produce bacteriocins exhibiting colicin M-like hydrolase activity towards peptidoglycan precursors
    Hélène Barreteau
    Universite Paris Sud, Institut de Biochimie et Biophysique Moleculaire et Cellulaire, UMR 8619, Orsay F 91405, France
    J Bacteriol 191:3657-64. 2009
    ..aeruginosa strains. These proteins thus represent a new class of bacteriocins (pyocins), the first ones reported thus far in the genus Pseudomonas that target peptidoglycan metabolism...
  11. ncbi The cytotoxic domain of colicin E9 is a channel-forming endonuclease
    Khedidja Mosbahi
    School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, UK
    Nat Struct Biol 9:476-84. 2002
    ..Our data suggest that endonuclease domains of colicins may mediate their own translocation across the bacterial inner membrane through an intrinsic channel activity ..
  12. pmc The colicin Ia receptor, Cir, is also the translocator for colicin Ia
    Karen S Jakes
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Mol Microbiol 75:567-78. 2010
    ..for the colicin to cross the outer membrane, but no 'second receptor' has ever been identified for TonB-dependent colicins, such as Ia...
  13. pmc The structural and energetic basis for high selectivity in a high-affinity protein-protein interaction
    Nicola A G Meenan
    Department of Biology, P O Box 373, University of York, York, YO10 5YW, United Kingdom
    Proc Natl Acad Sci U S A 107:10080-5. 2010
    ....
  14. ncbi Structural and mechanistic basis of immunity toward endonuclease colicins
    C Kleanthous
    School of Biological Sciences, University of East Anglia, Norwich, UK
    Nat Struct Biol 6:243-52. 1999
    ..This hyperefficient mechanism of inhibition could be well suited to other toxic enzyme systems, particularly where the substrate is a polymer extending beyond the boundaries of the active site...
  15. ncbi Purification and characterization of colicin V from Escherichia coli culture supernatants
    M J Fath
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115
    Biochemistry 33:6911-7. 1994
    ..0 amu, consistent with the mass of the unmodified 88 amino acid polypeptide. The purification scheme provides a rapid and simple way to obtain ColV for further biochemical analysis...
  16. ncbi The TolA-recognition site of colicin N. ITC, SPR and stopped-flow fluorescence define a crucial 27-residue segment
    I Gokce
    Department of Chemistry Faculty of Science, Gaziomanpasa University, Tokat, Turkey
    J Mol Biol 304:621-32. 2000
    b>Colicins translocate across the Escherichia coli outer membrane and periplasm by interacting with several receptors...
  17. pmc Competitive interactions in Escherichia coli populations: the role of bacteriocins
    Hadeel Majeed
    Zuckerberg Institute for Water Research, J Blaustein Institutes for Desert Research, Ben Gurion University, Sde Boqer Campus, Israel
    ISME J 5:71-81. 2011
    ..Two Escherichia coli strains were generated, each carrying a DNA-degrading bacteriocin (colicins E2 and E7)...
  18. doi Colicins exploit native disorder to gain cell entry: a hitchhiker's guide to translocation
    Daniel A Bonsor
    Department of Biology Area 10, University of York, Heslington, York, UK
    Biochem Soc Trans 36:1409-13. 2008
    ..One such group of toxins are enzymatic E colicins, protein antibiotics produced by Escherichia coli in times of stress...
  19. ncbi Colicins and microcins: the next generation antimicrobials
    Osnat Gillor
    Department of Ecology and Evolutionary Biology, Yale University, New Haven, Connecticut 06520 8106, USA
    Adv Appl Microbiol 54:129-46. 2004
  20. pmc Crystal structures of the OmpF porin: function in a colicin translocon
    Eiki Yamashita
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907 1392, USA
    EMBO J 27:2171-80. 2008
    The OmpF porin in the Escherichia coli outer membrane (OM) is required for the cytotoxic action of group A colicins, which are proposed to insert their translocation and active domains through OmpF pores...
  21. ncbi Clusters in an intrinsically disordered protein create a protein-binding site: the TolB-binding region of colicin E9
    Kaeko Tozawa
    School of Chemical Sciences and Pharmacy, University of East Anglia, Norwich NR4 7TJ, United Kingdom
    Biochemistry 44:11496-507. 2005
    ....
  22. ncbi Bacteriocin diversity and the frequency of multiple bacteriocin production in Escherichia coli
    David M Gordon
    School of Botany and Zoology, The Australian National University, Canberra, ACT 0200, Australia
    Microbiology 152:3239-44. 2006
    ..production of mitomycin C-inducible bacteriocins and screened using a PCR-based method for the presence of eleven colicins and seven microcins. Eight different colicins were detected and all seven microcins...
  23. pmc Investigating early events in receptor binding and translocation of colicin E9 using synchronized cell killing and proteolytic cleavage
    Ying Zhang
    School of Molecular Medical Sciences, Institute of Infection, Immunity and Inflammation, Centre for Biomolecular Sciences, University Park, Nottingham NG7 2RD, United Kingdom
    J Bacteriol 190:4342-50. 2008
    Enzymatic colicins such as colicin E9 (ColE9) bind to BtuB on the cell surface of Escherichia coli and rapidly recruit a second coreceptor, either OmpF or OmpC, through which the N-terminal natively disordered region (NDR) of their ..
  24. ncbi Local dispersal promotes biodiversity in a real-life game of rock-paper-scissors
    Benjamin Kerr
    Department of Biological Sciences, Stanford University, Stanford, California 94305, USA
    Nature 418:171-4. 2002
    ..We find that diversity is rapidly lost in our experimental community when dispersal and interaction occur over relatively large spatial scales, whereas all populations coexist when ecological processes are localized...
  25. ncbi The conserved asparagine in the HNH motif serves an important structural role in metal finger endonucleases
    Hsinchin Huang
    Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, ROC
    J Mol Biol 368:812-21. 2007
    ....
  26. pmc Dietary inclusion of colicin e1 is effective in preventing postweaning diarrhea caused by F18-positive Escherichia coli in pigs
    S A Cutler
    Department of Animal Science, Iowa State University, Ames, USA
    Antimicrob Agents Chemother 51:3830-5. 2007
    ..The dietary inclusion of colicin E1 may be an effective alternative to conventional antibiotics in the diets of weaning pigs for the prevention of PWD caused by F18-positive enterotoxigenic E. coli...
  27. pmc Deciphering the catalytic domain of colicin M, a peptidoglycan lipid II-degrading enzyme
    Hélène Barreteau
    Universite Paris Sud 11, UMR 8619, Institut de Biochimie et Biophysique Moleculaire et Cellulaire, 91405 Orsay
    J Biol Chem 285:12378-89. 2010
    ..Most of these residues are surface-exposed and located relatively close to each other, hence suggesting they belong to the colicin M active site...
  28. ncbi DNA binding and cleavage by the HNH homing endonuclease I-HmuI
    Betty W Shen
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave N A3 025, Seattle, WA 98109, USA
    J Mol Biol 342:43-56. 2004
    ..surface found in the I-PpoI homing endonuclease and an associated HNH/N active site found in the bacterial colicins, and a C-terminal DNA-binding domain previously observed in the I-TevI homing endonuclease...
  29. pmc Crystal structure of colicin M, a novel phosphatase specifically imported by Escherichia coli
    Kornelius Zeth
    Department of Protein Evolution, Max Planck Institute for Developmental Biology, Spemannstrasse 35, Tubingen, Germany
    J Biol Chem 283:25324-31. 2008
    b>Colicins are cytotoxic proteins secreted by certain strains of Escherichia coli...
  30. pmc Distinct conformational stability and functional activity of four highly homologous endonuclease colicins
    Ewald T J van den Bremer
    Department of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, 3584 CA Utrecht, The Netherlands e t j
    Protein Sci 13:1391-401. 2004
    ..Our results indicate that the apo-forms of these colicins are at room temperature and neutral pH in a dynamic conformational equilibrium between at least two quite ..
  31. ncbi Processing of DNase domain during translocation of colicin E7 across the membrane of Escherichia coli
    C C Liao
    Institute of Biochemistry, National Yang Ming University, Shih-Pai, Taipei, Taiwan 11221, Republic of China
    Biochem Biophys Res Commun 284:556-62. 2001
    ..These results suggest that the processing of ColE7 has occurred for translocation of the DNase-type colicin across the membrane and the process is probably taking place in the periplasmic space of the membrane...
  32. pmc CbrA is a flavin adenine dinucleotide protein that modifies the Escherichia coli outer membrane and confers specific resistance to Colicin M
    Stephanie Helbig
    Max Planck Institute for Developmental Biology, Department of Protein Evolution, Tuebingen, Germany
    J Bacteriol 194:4894-903. 2012
    ..The next homologs with the highest identity (over 50%) are found in the anaerobic Clostridium botulinum group 1 and a few other Firmicutes...
  33. ncbi Change of thermal stability of colicin E7 triggered by acidic pH suggests the existence of unfolded intermediate during the membrane-translocation phase
    K F Chak
    Institute of Biochemistry, National Yang Ming University, Taipei, Taiwan, ROC
    Proteins 32:17-25. 1998
    ..guanidine hydrochloride, indicating that this protein complex is indeed formed by aggregation of the monomeric colicins. Most interestingly, the aggregated colicins still perform in vivo bacteriocidal activity...
  34. pmc Enhancement of Shiga toxin production in enterohemorrhagic Escherichia coli serotype O157:H7 by DNase colicins
    Hirono Toshima
    Graduate School of Human Life Science, Osaka City University, 3 3 138 Sugimoto, Osaka 558 8585, Japan
    Appl Environ Microbiol 73:7582-8. 2007
    b>Colicins are proteins produced by and active against several strains of Escherichia coli...
  35. ncbi Concerted folding and binding of a flexible colicin domain to its periplasmic receptor TolA
    Gregor Anderluh
    Department of Biology, Biotechnical Faculty, University of Ljubljana, Vecna pot 111, 1000 Ljubljana, Slovenia
    J Biol Chem 278:21860-8. 2003
    ..Such domains are common features of all colicins and are required for their translocation across the outer membrane of the target Escherichia coli cell...
  36. ncbi Cys32 and His105 are the critical residues for the calcium-dependent cysteine proteolytic activity of CvaB, an ATP-binding cassette transporter
    Kai Hui Wu
    Department of Biology, Georgia State University, Atlanta, Georgia 30303, USA
    J Biol Chem 279:901-9. 2004
    ....
  37. pmc The crystal structure of the TolB box of colicin A in complex with TolB reveals important differences in the recruitment of the common TolB translocation portal used by group A colicins
    Ying Zhang
    Institute of Infection, Immunity and Inflammation, School of Molecular Medical Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, UK
    Mol Microbiol 75:623-36. 2010
    Interaction of the TolB box of Group A colicins with the TolB protein in the periplasm of Escherichia coli cells promotes transport of the cytotoxic domain of the colicin across the cell envelope...
  38. pmc Energy-dependent immunity protein release during tol-dependent nuclease colicin translocation
    Mireille Vankemmelbeke
    School of Molecular Medical Sciences and Institute of Infection, Immunity, and Inflammation, Centre for Biomolecular Sciences, University of Nottingham, Nottingham NG7 2RD, United Kingdom
    J Biol Chem 284:18932-41. 2009
    Nuclease colicins bind their target receptor in the outer membrane of sensitive cells in the form of a high affinity complex with their cognate immunity proteins...
  39. pmc Probing metal ion binding and conformational properties of the colicin E9 endonuclease by electrospray ionization time-of-flight mass spectrometry
    Ewald T J van den Bremer
    Department of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands
    Protein Sci 11:1738-52. 2002
    ..In addition, our metal ion binding studies and gas-phase dissociation experiments of the ternary E9 DNase-Zn(2+)-Im9 complex have provided further evidence that electrostatic interactions govern the gas phase ion stability...
  40. pmc Toxicity of the colicin M catalytic domain exported to the periplasm is FkpA independent
    Aurélie Barnéoud-Arnoulet
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Microbiologie de la Mediterranee, CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Bacteriol 192:5212-9. 2010
    ..Like the full-length protein, this domain is not active in the presence of the immunity protein Cmi. On the other hand, it does not require FkpA for toxic activity...
  41. ncbi Antibiotic-mediated antagonism leads to a bacterial game of rock-paper-scissors in vivo
    Benjamin C Kirkup
    Department of Ecology and Evolutionary Biology, Yale University, New Haven, Connecticut 06511, USA
    Nature 428:412-4. 2004
    b>Colicins are narrow-spectrum antibiotics produced by and active against Escherichia coli and its close relatives...
  42. pmc Escherichia coli bacteriocins: antimicrobial efficacy and prevalence among isolates from patients with bacteraemia
    Maruska Budic
    Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia
    PLoS ONE 6:e28769. 2011
    ..Escherichia coli produces two types of bacteriocins, colicins and microcins...
  43. doi Colicin M hydrolyses branched lipids II from Gram-positive bacteria
    Delphine Patin
    Univ Paris Sud, Laboratoire des Enveloppes Bactériennes et Antibiotiques, UMR 8619, Orsay, F 91405, France
    Biochimie 94:985-90. 2012
    ....
  44. ncbi Colicins and their potential in cancer treatment
    Lorna E Lancaster
    Institutes of Molecular Biology, University of Witten Herdecke, Stockumer Str 10, 58448 Witten, Germany
    Blood Cells Mol Dis 38:15-8. 2007
    b>Colicins are a family of antibacterial cytotoxins produced by Escherichia coli and released into the environment to reduce competition from other bacterial strains...
  45. ncbi Sensitivity of Shiga toxin-producing Escherichia coli (STEC) strains for colicins under different experimental conditions
    B J Jordi
    Bacteriology Division, Department of Infectious Diseases and Immunology, Utrecht University, Utrecht, The Netherlands
    FEMS Microbiol Lett 204:329-34. 2001
    Twenty Escherichia coli strains producing well-characterised colicins were tested for their inhibitory activity against five Shiga toxin-producing E. coli (STEC) strains using different media under aerobic and anaerobic conditions...
  46. ncbi The modes of action of colicins E5 and D, and related cytotoxic tRNases
    Haruhiko Masaki
    Department of Biotechnology, The University of Tokyo, Yayoi 1 1 1, Bunkyo ku, Tokyo 113 8657, Japan
    Biochimie 84:433-8. 2002
    b>Colicins E5 and D cleave the anticodon loops of distinct tRNAs of Escherichia coli both in vivo and in vitro, which accounts for their bactericidal actions through depletion of tRNAs and prevention of protein synthesis...
  47. pmc Inhibitory activities of colicins against Escherichia coli strains responsible for postweaning diarrhea and edema disease in swine
    Chad H Stahl
    Department of Animal Science, Iowa State University, Ames, Iowa 50011, USA
    Antimicrob Agents Chemother 48:3119-21. 2004
    The efficacies of colicins E1 and N against Escherichia coli strains responsible for postweaning diarrhea and edema disease, two of the most prevalent disease problems for pigs in the United States, were determined in vitro...
  48. pmc Translocation of inserted foreign epitopes by a channel-forming protein
    K S Jakes
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Proc Natl Acad Sci U S A 95:4321-6. 1998
    ..b>Colicins containing either epitope retain significant bactericidal activity and form channels of normal conductance in ..
  49. ncbi In vitro assessment of the cytotoxicity of nisin, pediocin, and selected colicins on simian virus 40-transfected human colon and Vero monkey kidney cells with trypan blue staining viability assays
    S E Murinda
    Department of Food Science, Pennsylvania State University, University Park, Pennsylvania 16802, USA
    J Food Prot 66:847-53. 2003
    Gram-positive bacterial bacteriocins (nisin and pediocin) and gram-negative bacterial bacteriocins (colicins [Col] E1, E3, E6, E7, and K) were evaluated for cytotoxicity against cultured simian virus 40-transfected human colon (SV40-HC) ..
  50. pmc Complete DNA sequence of a ColBM plasmid from avian pathogenic Escherichia coli suggests that it evolved from closely related ColV virulence plasmids
    Timothy J Johnson
    Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, 1802 Elwood Drive, VMRI 2, Iowa State University, Ames, IA 50011, USA
    J Bacteriol 188:5975-83. 2006
    ..These two F-type plasmids share remarkable similarity, except that they encode the production of different colicins; pAPEC-O2-ColV contains an intact ColV operon, and pAPEC-O1-ColBM encodes the colicins B and M...
  51. pmc Structural and functional alterations of a colicin-resistant mutant of OmpF porin from Escherichia coli
    D Jeanteur
    European Molecular Biology Laboratory, Heidelberg, Germany
    Proc Natl Acad Sci U S A 91:10675-9. 1994
    ....
  52. doi Following evolutionary paths to protein-protein interactions with high affinity and selectivity
    Kalia Bernath Levin
    Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, Israel
    Nat Struct Mol Biol 16:1049-55. 2009
    ....
  53. ncbi A mechanism for toxin insertion into membranes is suggested by the crystal structure of the channel-forming domain of colicin E1
    P Elkins
    Protein Engineering, Department Genentech, Inc 460 Pt San Bruno Blvd, South San Francisco, CA 94080, USA
    Structure 5:443-58. 1997
    Channel-forming colicins, including colicin E1, are a sub-family of bacteriocins...
  54. ncbi Importation of nuclease colicins into E coli cells: endoproteolytic cleavage and its prevention by the immunity protein
    Miklos de Zamaroczy
    Institut de Biologie Physico Chimique, CNRS, UPR 9073, 13, rue Pierre et Marie Curie 75005, Paris, France
    Biochimie 84:423-32. 2002
    A major group of colicins comprises molecules that possess nuclease activity and kill sensitive cells by cleaving RNA or DNA...
  55. pmc Colicin E3 cleavage of 16S rRNA impairs decoding and accelerates tRNA translocation on Escherichia coli ribosomes
    Lorna E Lancaster
    Institute of Molecular Biology, University of Witten Herdecke, 58448 Witten, Germany
    Mol Microbiol 69:390-401. 2008
    ..The tRNA-mRNA translocation is faster on colicin E3-cleaved than on intact ribosomes and is less sensitive to inhibition by the antibiotic viomycin...
  56. ncbi Lipid dependence of the channel properties of a colicin E1-lipid toroidal pore
    Alexander A Sobko
    A N Belozersky Institute of Physico Chemical Biology, Moscow State University, 119992 Moscow, Russia
    J Biol Chem 281:14408-16. 2006
    ....
  57. ncbi Characterization of colicinogenic Escherichia coli strains inhibitory to enterohemorrhagic Escherichia coli
    Gerry P Schamberger
    Department of Food Science and Nutrition, University of Minnesota, 1334 Eckles Avenue, St Paul, Minnesota 55108, USA
    J Food Prot 67:486-92. 2004
    ..The remaining set of 14 strains were evaluated for the presence of previously identified colicins. Seven colicins (B, El, E2/E7, E7, Ia/Ib, K, and M) were detected...
  58. ncbi Structural dynamics of the membrane translocation domain of colicin E9 and its interaction with TolB
    Emily S Collins
    School of Chemical Sciences, University of East Anglia, Norwich NR4 7TJ, UK
    J Mol Biol 318:787-804. 2002
    ..The flexibility of the translocation domain of colicin E9 may be connected with its need to recognise protein partners that assist it in crossing the outer membrane and in the translocation event itself...
  59. ncbi Ligand-induced changes in the conformational dynamics of a bacterial cytotoxic endonuclease
    Ewald T J van den Bremer
    Department of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands
    Biochemistry 43:4347-55. 2004
    ..In the present work we investigated the dynamic properties of the enzymatic domain of DNase colicins via time-resolved fluorescence and anisotropy decay analysis in combination with steady-state acrylamide ..
  60. pmc Sizing the protein translocation pathway of colicin Ia channels
    Paul K Kienker
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA
    J Gen Physiol 122:161-76. 2003
    ..Thus, the pathway for translocation is >/=26 A in diameter, or can stretch to this value. This is large enough for an alpha-helical hairpin to fit through...
  61. ncbi Identification of an essential cleavage site in ColE7 required for import and killing of cells
    Zhonghao Shi
    Institute of Biochemistry, National Yang Ming University, Taipei 11221, Taiwan and Institute of Molecular Biology, Academia Sinica, Taipei 11529, Taiwan
    J Biol Chem 280:24663-8. 2005
    ..Nuclease colicins are processed during translocation with only the cytotoxic nuclease domains traversing the inner membrane to ..
  62. ncbi The Tol/Pal system function requires an interaction between the C-terminal domain of TolA and the N-terminal domain of TolB
    Anne Walburger
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Biologie Structurale et Microbiologie, CNRS 31, Chemin Joseph Aiguier, Marseille, France
    Mol Microbiol 44:695-708. 2002
    ..It is involved in maintaining the integrity of the outer membrane, and is required for the uptake of group A colicins and DNA of filamentous bacteriophages...
  63. ncbi Colicins produced by the Escherichia fergusonii strains closely resemble colicins encoded by Escherichia coli
    David Smajs
    Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School, Houston, TX, USA
    FEMS Microbiol Lett 208:259-62. 2002
    ..fergusonii strain EF36 (pColE1-EF36) was found to be only partially immune to E1 colicins produced by two other E. fergusonii strains suggesting that pColE1-EF36 may represent an ancestor ColE1 plasmid.
  64. pmc The zinc ion in the HNH motif of the endonuclease domain of colicin E7 is not required for DNA binding but is essential for DNA hydrolysis
    Wen Yen Ku
    Graduate Institute of Life Science, National Defense Medical Center, Taipei, Taiwan 11472, Republic of China
    Nucleic Acids Res 30:1670-8. 2002
    ....
  65. ncbi The Tol proteins of Escherichia coli and their involvement in the translocation of group A colicins
    Jean Claude Lazzaroni
    Unité de Microbiologie et Génétique, UMR5122 CNRS INSA, Universite Lyon 1, Bâtiment André Lwoff, 10, rue Dubois, 69622 Villeurbanne Cedex, France
    Biochimie 84:391-7. 2002
    ..The Tol proteins have been parasitized by group A colicins for their translocation through the cell envelope...
  66. pmc A cka-gfp transcriptional fusion reveals that the colicin K activity gene is induced in only 3 percent of the population
    Janez Mulec
    Karst Research Institute, Scientific Centre of the Slovenian Academy of Sciences and Arts, Postojna, Slovenia
    J Bacteriol 185:654-9. 2003
    ..Expression of the cka-gfp fusion in a lexA-defective strain and in a relA spoT mutant strain indicates that differential expression of cka is established primarily at the level of transcription...
  67. pmc Crystal structural analysis and metal-dependent stability and activity studies of the ColE7 endonuclease domain in complex with DNA/Zn2+ or inhibitor/Ni2+
    Lyudmila G Doudeva
    Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan 11529, Republic of China
    Protein Sci 15:269-80. 2006
    ....
  68. pmc Colicin occlusion of OmpF and TolC channels: outer membrane translocons for colicin import
    Stanislav D Zakharov
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana, USA
    Biophys J 87:3901-11. 2004
    The interaction of colicins with target cells is a paradigm for protein import. To enter cells, bactericidal colicins parasitize Escherichia coli outer membrane receptors whose physiological purpose is the import of essential metabolites...
  69. ncbi The colicin G, H and X determinants encode microcins M and H47, which might utilize the catecholate siderophore receptors FepA, Cir, Fiu and IroN
    S I Patzer
    Mikrobiologie Membranphysiologie, Universitat Tubingen, Auf der Morgenstelle 28, Tubingen, Germany
    Microbiology 149:2557-70. 2003
    ..These microcins could aid the producing strain in competing against enterobacteria that utilize catecholate siderophores...
  70. ncbi Colicin crystal structures: pathways and mechanisms for colicin insertion into membranes
    Stanislav D Zakharov
    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907 1392, USA
    Biochim Biophys Acta 1565:333-46. 2002
    The X-ray structures of the channel-forming colicins Ia and N, and endoribonucleolytic colicin E3, as well as of the channel domains of colicins A and E1, and spectroscopic and calorimetric data for intact colicin E1, are discussed in the ..
  71. ncbi Calorimetric dissection of colicin DNase--immunity protein complex specificity
    Anthony H Keeble
    Department of Biology Area 10, Post Office Box 373, University of York, Heslington, York YO10 5YW, United Kingdom
    Biochemistry 45:3243-54. 2006
    ..capacity (DeltaC(p)) accompanying binding of each Im protein (Im2, Im7, Im8, and Im9) to the DNase domains of colicins E2, E7, E8, and E9, in the context of both cognate and noncognate complexes...
  72. ncbi Exoproducts of the Escherichia coli strain H22 inhibiting some enteric pathogens both in vitro and in vivo
    L Cursino
    Department of General Biology, ICB Universidade Federal de Minas Gerais, Belo Horizonte MG, Brazil
    J Appl Microbiol 100:821-9. 2006
    ..The antagonistic activity of the Escherichia coli strain H22 against enteric bacteria was studied both in vitro and in vivo...
  73. ncbi The crystal structure of the nuclease domain of colicin E7 suggests a mechanism for binding to double-stranded DNA by the H-N-H endonucleases
    Yi Sheng Cheng
    Institute of Molecular Biology, Academia Sinica, Taipei 11529, Taiwan, ROC
    J Mol Biol 324:227-36. 2002
    ..A mechanism for the binding of double-stranded DNA by dimeric H-N-H nuclease ColE7 is suggested...
  74. ncbi Ultraviolet resonance Raman studies reveal the environment of tryptophan and tyrosine residues in the native and partially folded states of the E colicin-binding immunity protein Im7
    Iñigo R Rodriguez-Mendieta
    Institute of Molecular Biophysics and Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, United Kingdom
    Biochemistry 44:3306-15. 2005
    ..We corroborated this observation using tryptophan-containing model compounds and conclude that the conventional interpretation of this UVRR feature at these wavelengths is unreliable...
  75. ncbi Human tumor cells are selectively inhibited by colicins
    J Chumchalova
    Department of Biology, Faculty of Medicine, Masaryk University, 66243 Brno, Czechia
    Folia Microbiol (Praha) 48:111-5. 2003
    The activity in vitro of four types of colicins (A, E1, E3, U) against one human standard fibroblast line and against 11 human tumor-cell lines carrying defined mutations of the p53 gene was quantified by MTT (tetrazolium bromide) assay...
  76. pmc TolA: a membrane protein involved in colicin uptake contains an extended helical region
    S K Levengood
    Department of Biochemistry, Duke University Medical Center, Durham, NC 27710
    Proc Natl Acad Sci U S A 88:5939-43. 1991
    The group A colicins and the DNA of many single-stranded filamentous bacteriophage are able to use combinations of the Tol proteins to gain entrance into or across the membrane of Escherichia coli...
  77. ncbi Structure of the complex of the colicin E2 R-domain and its BtuB receptor. The outer membrane colicin translocon
    Onkar Sharma
    Department of Biological Sciences, Lilly Hall of Life Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    J Biol Chem 282:23163-70. 2007
    ..It was concluded that the oblique orientation of the R-domain of the nuclease E colicins has a function in the recruitment of another member(s) of an outer membrane translocon...
  78. ncbi Immunity proteins and their specificity for endonuclease colicins: telling right from wrong in protein-protein recognition
    C Kleanthous
    School of Biological Sciences, University of East Anglia, Norwich, UK
    Mol Microbiol 28:227-33. 1998
    Immunity proteins inhibit colicins, protein toxins released by bacteria during times of environmental stress, by binding and inactivating their cytotoxic domains...
  79. ncbi Structure of the membrane-pore-forming fragment of colicin A
    M W Parker
    European Molecular Biology Laboratory, Heidelberg, FRG
    Nature 337:93-6. 1989
    b>Colicins are antibiotic proteins produced by and active against sensitive Escherichia coli and closely related bacteria...
  80. ncbi The role of electrostatics in colicin nuclease domain translocation into bacterial cells
    Daniel Walker
    Department of Biology, University of York, York YO10 5YW, United Kingdom
    J Biol Chem 282:31389-97. 2007
    The mechanism(s) by which nuclease colicins translocate distinct cytotoxic enzymes (DNases, rRNases, and tRNases) to the cytoplasm of Escherichia coli is unknown...
  81. ncbi Host gastro-intestinal dynamics and the frequency of colicin production by Escherichia coli
    Belinda Barnes
    National Centre for Epidemiology and Population Health, Australian National University, Canberra, ACT 0200, Australia
    Microbiology 153:2823-7. 2007
    The production of antimicrobial compounds known as colicins has been shown to be an important mediator of competitive interactions among Escherichia coli genotypes...
  82. doi Anomalous proton selectivity in a large channel: colicin A
    Stephen L Slatin
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Biochemistry 47:1778-88. 2008
    Some of the bactericidal proteins known as colicins exert their toxic action by forming a large, nonselective channel in the inner membrane of target bacteria. The structure of this channel is unknown...
  83. doi The SOS response affects thermoregulation of colicin K synthesis
    Matej Butala
    Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia
    FEMS Microbiol Lett 283:104-11. 2008
    ..This study investigated the effect of temperature on synthesis of Escherichia coli colicins E1, K, N and E7 as well as the molecular basis underlying thermoregulation of the colicin K activity gene cka...
  84. ncbi A novel transposon-like structure carries the genes for pyocin AP41, a Pseudomonas aeruginosa bacteriocin with a DNase domain homology to E2 group colicins
    Y Sano
    Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan
    Mol Gen Genet 237:161-70. 1993
    ..component (the deoxyribonuclease domain) and the immunity protein show remarkable homology to those of E2 group colicins, suggesting that these bacteriocins, which are produced by distantly related species, have originated from a ..
  85. pmc The role of SOS boxes in enteric bacteriocin regulation
    Osnat Gillor
    Department of Environmental Hydrology and Microbiology, Zuckerberg Institute for Water Research, J Blaustein Institutes for Desert Research, Ben Gurion University of the Negev, Sede Boqer Campus, 84990 Midreshet Ben Gurion, Israel
    Microbiology 154:1783-92. 2008
    Bacteriocins are a large and functionally diverse family of toxins found in all major lineages of Bacteria. Colicins, those bacteriocins produced by Escherichia coli, serve as a model system for investigations of bacteriocin structure-..
  86. pmc Hot-spot residues at the E9/Im9 interface help binding via different mechanisms
    Sergio E Wong
    Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, CA 92093 0365, USA
    Biopolymers 89:916-20. 2008
    ..This type of model may be helpful in engineering hot-spot clusters at protein-protein interfaces and, consequently, the design of specificity...
  87. ncbi Evolutionary relationship of uptake systems for biopolymers in Escherichia coli: cross-complementation between the TonB-ExbB-ExbD and the TolA-TolQ-TolR proteins
    V Braun
    Mikrobiologie II, Universitat Tubingen, Germany
    Mol Microbiol 8:261-8. 1993
    ..Group B colicins, ferric siderophores and vitamin B12 are taken up via the TonB-ExbB-ExbD, group A colicins via the TolA-TolQ-TolR ..
  88. pmc Role of TolR N-terminal, central, and C-terminal domains in dimerization and interaction with TolA and tolQ
    L Journet
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Biologie Structurale et Microbiologie, Centre National de la Recherche Scientifique, 13402 Marseille Cedex 20, France
    J Bacteriol 181:4476-84. 1999
    ..a multiprotein system involved in maintaining the cell envelope integrity and is necessary for the import of some colicins and phage DNA into the bacterium...
  89. pmc DNA and amino acid sequence analysis of structural and immunity genes of colicins Ia and Ib
    J A Mankovich
    J Bacteriol 168:228-36. 1986
    The nucleotide sequences for colicin Ia and colicin Ib structural and immunity genes were determined. The two colicins each consist of 626 amino acid residues...
  90. pmc Nucleotide sequences of the tolA and tolB genes and localization of their products, components of a multistep translocation system in Escherichia coli
    S K Levengood
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710
    J Bacteriol 171:6600-9. 1989
    ..tolQRAB gene cluster of Escherichia coli render the bacteria tolerant to high concentrations of the E, A, or K colicins as well as tolerant to infection by the single-stranded filamentous bacteriophage. The nucleotide sequence of a 2...
  91. pmc Colicin cleavage by OmpT protease during both entry into and release from Escherichia coli cells
    D Cavard
    Centre de Biochimie et de Biologie Moleculaire, Centre National de la Recherche Scientifique, Marseille, France
    J Bacteriol 172:648-52. 1990
    Proteolysis of colicins A, E1, E2, and E3 was observed after they were added to whole cells carrying a functional ompT gene. Recombinant plasmid pML19 containing the ompT gene enabled two mutant strains to cleave the added colicins...
  92. ncbi Studies on colicin B translocation: FepA is gated by TonB
    Surendranathan Devanathan
    School of Molecular Biosciences, Washington State University, Pullman, WA 99164 4234, USA
    Mol Microbiol 65:441-53. 2007
    ..Unexpectedly, TonB was also required for colicin-induced exposure of the FepA TonB box, suggesting that TonB binds FepA at a different site prior to interaction with the TonB box...
  93. ncbi Mutational analyses define helix organization and key residues of a bacterial membrane energy-transducing complex
    Emilie L Goemaere
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Biologie Structurale et Microbiologie, CNRS UPR 9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Mol Biol 366:1424-36. 2007
    ..The Tol system is parasitized by bacterial toxins called colicins, which are imported through the outer membrane using an energy-independent process...
  94. ncbi Cleavage of colicin D is necessary for cell killing and requires the inner membrane peptidase LepB
    M de Zamaroczy
    Institut de Biologie Physico Chimique, CNRS, UPR 9073, 75005, Paris, France
    Mol Cell 8:159-68. 2001
    ..Catalytic colicins share a consensus sequence at their putative processing site...
  95. ncbi Using chimeric immunity proteins to explore the energy landscape for alpha-helical protein folding
    N Ferguson
    School of Biochemistry and Molecular Biology, University of Leeds, Leeds LS2 9JT, UK
    J Mol Biol 307:393-405. 2001
    ....
  96. ncbi Ultrarapid mixing experiments reveal that Im7 folds via an on-pathway intermediate
    A P Capaldi
    School of Biochemistry and Molecular Biology and the Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, UK
    Nat Struct Biol 8:68-72. 2001
    ..Quantitative kinetic modeling of folding and unfolding data acquired over a wide range of urea concentrations demonstrate that this intermediate ensemble lies on a direct path from the unfolded to the native state...
  97. ncbi The newly characterized colicin Y provides evidence of positive selection in pore-former colicin diversification
    M A Riley
    Department of Ecology and Evolutionary Biology, Yale University, New Haven, CT 06511, USA
    Microbiology 146:1671-7. 2000
    ..process of protein diversification of the large family of antimicrobial toxins of Escherichia coli, known as the colicins. Data from previous studies suggest that the relatively rare nuclease colicins appear to diversify primarily ..
  98. ncbi The structure of TolB, an essential component of the tol-dependent translocation system, and its protein-protein interaction with the translocation domain of colicin E9
    S Carr
    Colicin Research Group, School of Biological Sciences, School of Chemical Sciences, University of East Anglia, Norwich, NR4 7TJ, UK
    Structure 8:57-66. 2000
    ..The translocation of enzymatic E colicins like colicin E9 is dependent upon TolB but the details of the process are not known.
  99. ncbi The colicin E3 outer membrane translocon: immunity protein release allows interaction of the cytotoxic domain with OmpF porin
    Stanislav D Zakharov
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    Biochemistry 45:10199-207. 2006
    ....
  100. ncbi Ion-channel-forming colicins
    R M Stroud
    Department of Biochemistry and Biophysics, University of California, San Francisco School of Medicine 94143 0448, USA
    Curr Opin Struct Biol 8:525-33. 1998
    Several features of ion-channel-forming colicins have been illuminated by recent revelations: its four-domain structure, the mechanism and thermodynamics of binding to the gating loop of outer membrane porins, the mechanism of ..
  101. pmc Characterization of colicin S4 and its receptor, OmpW, a minor protein of the Escherichia coli outer membrane
    H Pilsl
    Mikrobiologie Membranphysiologie, Universitat Tubingen, Tubingen, Germany
    J Bacteriol 181:3578-81. 1999
    ..By contrast, the predicted receptor binding domain shows no sequence similarities to other colicins. Mutants that lacked the OmpW protein were resistant to colicin S4.

Research Grants66

  1. GATING OF PROTEIN CHANNELS IN LIPID BILAYER MEMBRANES
    Alan Finkelstein; Fiscal Year: 1993
    ..into membranes by bacterial proteins such as diphtheria toxin, tetanus toxin, botulinum toxin, anthrax toxin, and colicins of the E1 class (E1, Ia, Ib and A)...
  2. GATING OF PROTEIN CHANNELS IN LIPID BILAYER MEMBRANES
    Alan Finkelstein; Fiscal Year: 2010
    ..By forming chimeric channels by helix swapping between colicins Ia and A, and between diphtheria toxin's T-domain and colicin A, we shall identify which parts of colicin A ..
  3. DOMAIN MAPPING CLOSTRIDIUM PERFRINGENS THETA TOXIN
    Rodney Tweten; Fiscal Year: 2000
    ..Unlike many bacterial toxins, such as diphtheria toxin or the colicins, pH does not act as a trigger for the formation of a "molten globule" or insertion intermediate...
  4. GATING OF PROTEIN CHANNELS IN LIPID BILAYER MEMBRANES
    Alan Finkelstein; Fiscal Year: 1990
    ..those inserted into membranes by bacterial proteins, such as diptheria toxin, tetanus toxin, botulinum toxin, and colicins of the E1 class (E1, Ib, A and K), and those normally present in plasma membranes, such as the voltage-dependent ..
  5. LOOP DELETION MUTAGENESIS OF FEPA
    PHILLIP KLEBBA; Fiscal Year: 1999
    ..3. Conformation: binding of antibodies and colicins to surface loops, and maintenance of trimeric structure. 4...
  6. MOLECULAR MECHANISMS OF MEMBRANE TRANSPORT
    David Cafiso; Fiscal Year: 2003
    ..to the accumulation of nutrients, this class of proteins functions in the uptake of bactericidal agents, such as colicins, phages and small molecule antibiotics...
  7. STRUCTURE/FUNCTION OF MEMBRANE PROTEINS
    William Dowhan; Fiscal Year: 2000
    ..phospholipids have been implicated in protein translocation across the cytoplasmic membrane, the insertion of colicins into membranes, and the initiation of DNA synthhesis...
  8. STRUCTURE AND FUNCTION OF MEMBRANE PROTEINS
    William Dowhan; Fiscal Year: 1993
    ..phospholipids have been implicated in protein translocation across the cytoplasmic membrane, the insertion of colicins into membranes, and the initiation of DNA synthhesis...
  9. FLUID BILAYER INTERACTIONS WITH PEPTIDES
    Stephen White; Fiscal Year: 2004
    ..to various subcellular compartments? How can pH-induced refolding on membranes of diphtheria toxin T-domain and colicins cause highly charged helices to cross lipid bilayers? How can short, highly basic "Trojan" peptides - such as ..
  10. STRUCTURE & FUNCTION OF THE COLICIN E1 CHANNEL
    WILLIAM CRAMER; Fiscal Year: 1993
    ....
  11. Translocation through TonB-dependent transporters
    Karen Jakes; Fiscal Year: 2011
    ..Protein toxins called colicins that are made by some E. coli to kill other E...
  12. STRUCTURE/FUNCTION OF COLICIN E1/RECEPTOR/IMM PROTEIN
    WILLIAM CRAMER; Fiscal Year: 2002
    ..A weak interaction precludes effective binding. One that is too strong prevents insertion into the bilayer. ..
  13. BIOSYNTHESIS OF MEMBRANE PROTEINS
    Robert Webster; Fiscal Year: 1993
    ..role of the TolQRA and B proteins in maintaining membrane integrity and facilitating the uptake of the group A colicins will be investigated...
  14. THE MECHANISM OF ACTION OF COLICIN
    WILLIAM CRAMER; Fiscal Year: 1980
    ..The latter studies will be complemented by using a small 20 k dalton tryptic fragment of colicin E1 which is active in the synthetic lipid vesicles. ..
  15. TRANSPORT OF VITAMIN B12 IN E COLI
    ROBERT KADNER; Fiscal Year: 1993
    ..The relation of this process to the uptake of colicins, which utilize the B12 receptor will be explored, initially by examination of the requirement for btuC function ..
  16. Receptor-mediated Colicin Import
    WILLIAM CRAMER; Fiscal Year: 2007
    ..Receptor-mediated import of the toxin-like colicins is a paradigm for protein import to mitochondria and the import of viral proteins...