- Multidrug-resistance efflux pumps - not just for resistance
Laura J V Piddock
Antimicrobial Agents Research Group, Division of Immunity and Infection, The Medical School, University of Birmingham, Birmingham B15 2TT, UK
Nat Rev Microbiol 4:629-36. 2006
..Here, I present the accumulating evidence that multidrug-resistance efflux pumps have roles in bacterial pathogenicity and propose that these pumps therefore have greater clinical relevance than is usually attributed to them...
- Coupled degradation of a small regulatory RNA and its mRNA targets in Escherichia coli
Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Genes Dev 17:2374-83. 2003
..Thus, this large class of regulatory RNAs share an unexpected intrinsic mechanism for shutting off their action...
- A genomic view of the human-Bacteroides thetaiotaomicron symbiosis
Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St Louis, MO 63110, USA
Science 299:2074-6. 2003
..These and other expanded paralogous groups shed light on the molecular mechanisms underlying symbiotic host-bacterial relationships in our intestine...
- The stringent response and cell cycle arrest in Escherichia coli
Daniel J Ferullo
Department of Biology and Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA, USA
PLoS Genet 4:e1000300. 2008
..This is the first indication that bacterial chromosome segregation, whose mechanism is not understood, is a step that may be regulated in response to environmental conditions...
- Crystal structures of a multidrug transporter reveal a functionally rotating mechanism
Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567 0047, Japan
Nature 443:173-9. 2006
..The voluminous binding pocket is aromatic and allows multi-site binding. The structures indicate that drugs are exported by a three-step functionally rotating mechanism in which substrates undergo ordered binding change...
- The Lyme disease agent exploits a tick protein to infect the mammalian host
Sections of Rheumatology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
Nature 436:573-7. 2005
..scapularis drastically reduced the capacity of tick-borne spirochaetes to infect mice. These results show the capacity of a pathogen to use a secreted arthropod protein to help it colonize the mammalian host...
- TROSPA, an Ixodes scapularis receptor for Borrelia burgdorferi
Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520, USA
Cell 119:457-68. 2004
..Identification of an I. scapularis receptor for B. burgdorferi is the first step toward elucidating arthropod ligands that are required for survival of spirochetes in nature...
- The Acinetobacter baumannii 19606 OmpA protein plays a role in biofilm formation on abiotic surfaces and in the interaction of this pathogen with eukaryotic cells
Jennifer A Gaddy
Department of Microbiology, Miami University, Oxford, OH 45056, USA
Infect Immun 77:3150-60. 2009
..Interestingly, the killing activity of cell-free culture supernatants proved to be protease and temperature sensitive, suggesting that its cytotoxic activity is due to secreted proteins, some of which are different from OmpA...
- Outer-surface protein C of the Lyme disease spirochete: a protein induced in ticks for infection of mammals
Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South Fourth Street, Hamilton, MT 59840, USA
Proc Natl Acad Sci U S A 101:3142-7. 2004
..The induction of a spirochetal virulence factor preceding the time and host in which it is required demonstrates a developmental sequence for transmission of this arthropod-borne pathogen...
- Structure and function of bacterial outer membrane proteins: barrels in a nutshell
Biozentrum Basel, Abteilung Mikrobiologie, Klingelbergstr 50, CH 4056 Basel, Switzerland
Mol Microbiol 37:239-53. 2000
..Our review is aimed at discussing their common principles and peculiarities as well as open questions associated with them...
- Release of outer membrane vesicles by Gram-negative bacteria is a novel envelope stress response
Amanda J McBroom
Duke University Medical Center, Department of Biochemistry Box 3711, Durham, NC 27710, USA
Mol Microbiol 63:545-58. 2007
..In addition to identifying a novel mechanism for alleviating stress, this work provides physiological relevance for vesicle production as a protective mechanism...
- Membrane protein architects: the role of the BAM complex in outer membrane protein assembly
Timothy J Knowles
Cancer Research UK Institute for Cancer Studies, School of Cancer Sciences, Edgbaston, Birmingham, UK
Nat Rev Microbiol 7:206-14. 2009
..Here, we discuss how these structures illuminate the mechanisms responsible for the biogenesis of outer membrane proteins...
- Complexity and complementarity of outer membrane protein A recognition by cellular and humoral innate immunity receptors
Centre d Immunologie Pierre Fabre, 5 Avenue Napoleon III, 74164 Saint Julien en Genevois, France
Immunity 22:551-60. 2005
..Thus, SR-mediated KpOmpA recognition and TLR2-dependent cellular activation set in motion a nonredundant PTX3-mediated humoral amplification loop of innate immunity...
- Crystal structure of bacterial multidrug efflux transporter AcrB
Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567 0047, Japan
Nature 419:587-93. 2002
- Structures of gram-negative cell walls and their derived membrane vesicles
T J Beveridge
Canadian Bacterial Disease Network, and Department of Microbiology, College of Biological Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1
J Bacteriol 181:4725-33. 1999
- Outer membrane protein 38 of Acinetobacter baumannii localizes to the mitochondria and induces apoptosis of epithelial cells
Chul Hee Choi
Department of Microbiology, Kyungpook National University School of Medicine, Daegu 700 422, Korea
Cell Microbiol 7:1127-38. 2005
..These results demonstrate that Omp38 may act as a potential virulence factor to induce apoptosis of epithelial cells in the early stage of A. baumannii infection...
- Cloning and characterization of a gene encoding the major surface protein of the bacterial endosymbiont Wolbachia pipientis
H R Braig
Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, Connecticut 06520, USA
J Bacteriol 180:2373-8. 1998
..As such, this gene will be a very useful tool for Wolbachia strain typing and phylogenetic analysis, as well as understanding the molecular basis of the interaction of Wolbachia with its host...
- SigmaE-dependent small RNAs of Salmonella respond to membrane stress by accelerating global omp mRNA decay
Max Planck Institute for Infection Biology, Chariteplatz 1, 10117 Berlin, Germany
Mol Microbiol 62:1674-88. 2006
..In unstressed bacterial cells, the two oE-dependent small RNAs function within a surveillance loop to maintain envelope homeostasis and to achieve autoregulation of oE...
- SciN is an outer membrane lipoprotein required for type VI secretion in enteroaggregative Escherichia coli
Marie Stéphanie Aschtgen
Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Biologie Structurale et Microbiologie, CNRS, UPR 9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
J Bacteriol 190:7523-31. 2008
..Together, these results support a model in which SciN is an outer membrane lipoprotein exposed in the periplasm and essential for the Sci-1 apparatus function...
- The meningococcal vaccine candidate GNA1870 binds the complement regulatory protein factor H and enhances serum resistance
Section of Infectious Diseases, Boston University School of Medicine, Boston, MA 02118, USA
J Immunol 177:501-10. 2006
..These data provide further support for inclusion of this molecule in a meningococcal vaccine. To reflect the critical function of this molecule, we suggest calling it fH-binding protein...
- Prevalence and genetic characterization of pertactin-deficient Bordetella pertussis in Japan
Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan
PLoS ONE 7:e31985. 2012
..Our observations suggested that human host factors (genetic factors and immune status) that select for Prn(-) strains have arisen and that Prn expression is not essential for fitness under these conditions...
- β-Barrel membrane protein assembly by the Bam complex
Christine L Hagan
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
Annu Rev Biochem 80:189-210. 2011
- OspC is potent plasminogen receptor on surface of Borrelia burgdorferi
Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
J Biol Chem 287:16860-8. 2012
..The co-localization of plasminogen and OspC on OspC-expressing spirochetes further implicates OspC as a biologically relevant plasminogen receptor on the surface of live B. burgdorferi...
- Hfq-dependent regulation of OmpA synthesis is mediated by an antisense RNA
Klas I Udekwu
Department of Cell and Molecular Biology, Uppsala University, S 75124 Uppsala, Sweden
Genes Dev 19:2355-66. 2005
..MicA requires the presence of the Hfq protein, although the mechanistic basis for this remains unclear...
- Identification of a multicomponent complex required for outer membrane biogenesis in Escherichia coli
Department of Chemistry, Princeton University, Princeton, New Jersey 08544, USA
Cell 121:235-45. 2005
..These genetic interactions suggest a role for YfgL, one of the lipoprotein components of the protein assembly complex, in a homeostatic control mechanism that coordinates the overall OM assembly process...
- A phase 1 study of a meningococcal native outer membrane vesicle vaccine made from a group B strain with deleted lpxL1 and synX, over-expressed factor H binding protein, two PorAs and stabilized OpcA expression
P B Keiser
Walter Reed Army Institute of Research WRAIR, Silver Spring, MD, USA
Vaccine 29:1413-20. 2011
..Depletion assays show contributions to bactericidal activity from antibodies to lipooligosaccharide (LOS), fHbp v.1 and OpcA...
- Immunological and molecular polymorphisms of OspC, an immunodominant major outer surface protein of Borrelia burgdorferi
Max von Pettenkofer Institut fur Hygiene und Medizinische Mikrobiologie, Universitat Munchen, Germany
Infect Immun 61:2182-91. 1993
- Outer membrane proteins: key players for bacterial adaptation in host niches
Food Animal Health Research Program, Department of Veterinary Preventive Medicine, The Ohio State University, 1680 Madison Avenue, Wooster, OH 44691, USA
Microbes Infect 4:325-31. 2002
..In this paper, we will present a brief review on OMPs that contribute to bacterial adaptive responses including iron uptake, antimicrobial peptide resistance, serum resistance, and drug/bile resistance...
- Associations between virulence factors of Shiga toxin-producing Escherichia coli and disease in humans
Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada
J Clin Microbiol 37:497-503. 1999
- Multiplex real-time PCR for detection of anaplasma phagocytophilum and Borrelia burgdorferi
Joshua W Courtney
Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd, MS G 13, Atlanta, GA 30333, USA
J Clin Microbiol 42:3164-8. 2004
..phagocytophilum and Borrelia species, two of the most common tick-borne infectious agents in the United States...
- Outer membrane permeability and antibiotic resistance
Anne H Delcour
Department of Biology, University of Houston, 369 Science and Research Building II, Houston, TX 77204 5001, USA
Biochim Biophys Acta 1794:808-16. 2009
..This review will describe the molecular mechanisms for permeation of antibiotics through the outer membrane, and the strategies that bacteria have deployed to resist antibiotics by modifications of these pathways...
- Borrelia burgdorferi OspC protein required exclusively in a crucial early stage of mammalian infection
Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, MT 59840, USA
Infect Immun 74:3554-64. 2006
..We conclude that OspC is indispensable for establishing infection by B. burgdorferi in mammals but is not required at any other point of the mouse-tick infection cycle...
- The trans-envelope Tol-Pal complex is part of the cell division machinery and required for proper outer-membrane invagination during cell constriction in E. coli
Matthew A Gerding
Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, OH, USA
Mol Microbiol 63:1008-25. 2007
- Rickettsioses as paradigms of new or emerging infectious diseases
Unite des Rickettsies, Faculte de Medecine, CNRS UPRESA 6020, Marseille, France
Clin Microbiol Rev 10:694-719. 1997
..We also assess the pathogenic potential of rickettsial strains that to date have been associated only with arthropods, and we discuss diseases of unknown etiology that may be rickettsioses...
- Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis
R S Stephens
Program in Infectious Diseases, University of California, Berkeley, CA 94720, USA
Science 282:754-9. 1998
..The phylogenetic mosaic of chlamydial genes, including a large number of genes with phylogenetic origins from eukaryotes, implies a complex evolution for adaptation to obligate intracellular parasitism...
- Global emm type distribution of group A streptococci: systematic review and implications for vaccine development
Andrew C Steer
Centre for International Child Health, University of Melbourne, Australia
Lancet Infect Dis 9:611-6. 2009
- HHomp--prediction and classification of outer membrane proteins
Department of Protein Evolution, Max Planck Institute for Developmental Biology, Spemannstrasse 35, 72076 Tubingen, Germany
Nucleic Acids Res 37:W446-51. 2009
..In Escherichia coli, HHomp identifies 57 out of 59 known OMPs and correctly assigns them to their functional subgroups. HHomp can be accessed at http://toolkit.tuebingen.mpg.de/hhomp...
- Structure of a type IV secretion system core complex
Institute of Structural and Molecular Biology, School of Crystallography, Birkbeck College, Malet Street, London, WC1E 7HX, UK
Science 323:266-8. 2009
..Overall, the T4SS core complex structure is different in both architecture and composition from the other known double membrane-spanning secretion system that has been structurally characterized...
- Next-generation outer membrane vesicle vaccines against Neisseria meningitidis based on nontoxic LPS mutants
Peter van der Ley
Unit Vaccinology, National Institute of Public Health and the Environment RIVM, Bilthoven, The Netherlands
Hum Vaccin 7:886-90. 2011
..Several groups have now started the development of native OMV vaccines based on non-toxic LPS mutants, and this Commentary provides an overview of the various approaches and results thus far...
- Actinobacillus pleuropneumoniae: pathobiology and pathogenesis of infection
Janine T Bosse
Molecular Infectious Diseases Group, Department of Paediatrics, Faculty of Medicine, Imperial College, St Mary s Campus, W2 1PG, London, UK
Microbes Infect 4:225-35. 2002
- Tolerance to the antimicrobial peptide colistin in Pseudomonas aeruginosa biofilms is linked to metabolically active cells, and depends on the pmr and mexAB-oprM genes
Sünje Johanna Pamp
BioCentrum DTU, Technical University of Denmark, Lyngby, Denmark
Mol Microbiol 68:223-40. 2008
..Consequently, targeting the two physiologically distinct subpopulations by combined antimicrobial treatment with either ciprofloxacin and colistin or tetracycline and colistin almost completely eradicated all biofilm cells...
- Transcript profiling and inference of Escherichia coli K-12 ArcA activity across the range of physiologically relevant oxygen concentrations
Matthew D Rolfe
The Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom
J Biol Chem 286:10147-54. 2011
- Relationship between immunity to Borrelia burgdorferi outer-surface protein A (OspA) and Lyme arthritis
Allen C Steere
Center for Immunology and Inflammatory Diseases, Massachusetts General Hospital, Harvard Medical School, Boston, USA
Clin Infect Dis 52:s259-65. 2011
..Thus, a vaccine-induced immune response to OspA does not replicate the sequence of events needed in the natural infection to induce antibiotic-refractory Lyme arthritis...
- A molecular Swiss army knife: OmpA structure, function and expression
Stephen G J Smith
Department of Clinical Microbiology, Trinity College Dublin, St James s Hospital, Dublin, Ireland
FEMS Microbiol Lett 273:1-11. 2007
..Many of these properties are due to four short protein loops that emanate from the protein to the outside of the cell. Herein it is described how the structure of this protein relates to its many functions...
- SoxS regulates the expression of the Salmonella enterica serovar Typhimurium ompW gene
Laboratorio de Microbiologia Molecular, Departamento de Ciencias Biologicas, Universidad Andres Bello, Santiago, Chile
Microbiology 155:2490-7. 2009
..Transcriptional fusions support the relevance of this region in ompW activation. The SoxS site is in the forward orientation and its location suggests that the ompW gene has a class I SoxS-dependent promoter...
- Vaccination with outer membrane complexes elicits rapid protective immunity to multidrug-resistant Acinetobacter baumannii
Michael J McConnell
Unit of Infectious Disease, Microbiology, and Preventive Medicine, Hospitales Universitarios Virgen del Rocio, Edificio Laboratorio Anexo Caracola, Avenida Manuel Siurot s n, 41013 Seville, Spain
Infect Immun 79:518-26. 2011
..These results indicate that prophylactic vaccination and antibody-based therapies based on an outer membrane complex vaccine may be viable approaches to preventing the morbidity and mortality caused by this pathogen...
- Identification of a protein complex that assembles lipopolysaccharide in the outer membrane of Escherichia coli
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
Proc Natl Acad Sci U S A 103:11754-9. 2006
..We show that RlpB is also essential for cell viability and that the Imp/RlpB complex is responsible for LPS reaching the outer surface of the outer membrane...
- Evaluation of high-resolution typing methods for Chlamydia trachomatis in samples from heterosexual couples
Reinier J M Bom
Public Health Laboratory, Public Health Service of Amsterdam, Nieuwe Achtergracht 100, 1018 WT Amsterdam, The Netherlands
J Clin Microbiol 49:2844-53. 2011
..This shows that the chlamydial genome in clinical specimens has an appropriate polymorphism to enable epidemiological cluster analysis using MLST and MLVA...
- Identification and characterization of inhibitors of multidrug resistance efflux pumps in Pseudomonas aeruginosa: novel agents for combination therapy
Microcide Pharmaceuticals, Inc, Mountain View, California 94043, USA
Antimicrob Agents Chemother 45:105-16. 2001
..aeruginosa strains that are highly resistant to fluoroquinolones...
- Brucella abortus induces the secretion of proinflammatory mediators from glial cells leading to astrocyte apoptosis
Clara García Samartino
Instituto de Estudios de la Inmunidad Humoral IDEHU, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires Junín 956 4 Piso 1113 Buenos Aires, Argentina
Am J Pathol 176:1323-38. 2010
..Our results provide proof of the principle that Brucella lipoproteins could be key virulence factors in neurobrucellosis and that astrogliosis might contribute to neurobrucellosis pathogenesis...
- Crystal structure of the bacterial membrane protein TolC central to multidrug efflux and protein export
Department of Pathology, University of Cambridge, UK
Nature 405:914-9. 2000
..The structure provides an explanation of how the cell cytosol is connected to the external environment during export, and suggests a general mechanism for the action of bacterial efflux pumps...
- Structural basis for the regulated protease and chaperone function of DegP
Research Institute for Molecular Pathology IMP, Dr Bohrgasse 7, A 1030 Vienna, Austria
Nature 453:885-90. 2008
..Oligomer reassembly and concomitant activation on substrate binding may also be critical in regulating other HtrA proteases implicated in protein-folding diseases...
- Geographic uniformity of the Lyme disease spirochete (Borrelia burgdorferi) and its shared history with tick vector (Ixodes scapularis) in the Northeastern United States
Wei Gang Qiu
Department of Ecology and Evolution, State University of New York, Stony Brook, New York 11794 5245, USA
Genetics 160:833-49. 2002
..We therefore conclude that the genetic structure of B. burgdorferi has been intimately shaped by the natural history of its main vector, the northern lineage of I. scapularis ticks...
- Expression of Borrelia burgdorferi OspC and DbpA is controlled by a RpoN-RpoS regulatory pathway
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
Proc Natl Acad Sci U S A 98:12724-9. 2001
..burgdorferi. Our findings provide a foundation for elucidating further key regulatory networks that potentially impact many aspects of B. burgdorferi's parasitic strategy, host range, and virulence expression...
- A program of Yersinia enterocolitica type III secretion reactions is activated by specific signals
V T Lee
Department of Microbiology and Immunology, University of California Los Angeles School of Medicine, Los Angeles, California 90095, USA
J Bacteriol 183:4970-8. 2001
..Together these results suggest that yersiniae may have evolved distinct secretion reactions in response to environmental signals...
- Requirement of ArcA for redox regulation in Escherichia coli under microaerobic but not anaerobic or aerobic conditions
Laboratory for Microbiology, Swammerdam Institute for Life Sciences, University of Amsterdam, Nieuwe Achtergracht 166, 1018WV Amsterdam, The Netherlands
J Bacteriol 185:204-9. 2003
..Thus, the ArcA regulator was found to exert major control on flux distribution, and it is concluded that the ArcAB system should be considered a microaerobic redox regulator...
- A Pseudomonas syringae type III effector suppresses cell wall-based extracellular defense in susceptible Arabidopsis plants
Department of Energy Plant Research Laboratory and Department of Plant Biology, Michigan State University, East Lansing, MI 48824, USA
Proc Natl Acad Sci U S A 100:8577-82. 2003
..AvrPto is therefore one of the long postulated suppressors of an salicylic acid-independent, cell wall-based defense that is aimed at hrp mutant bacteria...
- Electrically conductive bacterial nanowires produced by Shewanella oneidensis strain MR-1 and other microorganisms
Yuri A Gorby
Pacific Northwest National Laboratory, Richland, WA 99352, USA
Proc Natl Acad Sci U S A 103:11358-63. 2006
- Acinetobacter baumannii outer membrane protein A targets the nucleus and induces cytotoxicity
Chul Hee Choi
Department of Microbiology, Kyungpook National University School of Medicine, Daegu, Korea
Cell Microbiol 10:309-19. 2008
..We propose a novel pathogenic mechanism of A. baumannii regarding the nuclear targeting of the bacterial structural protein AbOmpA...
- Proteomics in gram-negative bacterial outer membrane vesicles
Eun Young Lee
Department of Life Science and Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea
Mass Spectrom Rev 27:535-55. 2008
- The ArcBA two-component system of Escherichia coli is regulated by the redox state of both the ubiquinone and the menaquinone pool
Swammerdam Institute for Life Sciences Molecular Microbial Physiology, University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV Amsterdam, The Netherlands
J Bacteriol 192:746-54. 2010
..coli is modulated by the redox state of the menaquinone pool and that the ubiquinone/ubiquinol ratio in vivo surely is not the only determinant of ArcB activity...
- An ecological approach to preventing human infection: vaccinating wild mouse reservoirs intervenes in the Lyme disease cycle
Jean I Tsao
Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, CT 06520, USA
Proc Natl Acad Sci U S A 101:18159-64. 2004
..Thus, where nonmouse hosts play a large role in infection dynamics, vaccination should be directed at additional species...
- Identification and molecular cloning of a gene encoding a fibronectin-binding protein (CadF) from Campylobacter jejuni
M E Konkel
Department of Microbiology, Washington State University, Pullman 99164 4233, USA
Mol Microbiol 24:953-63. 1997
..jejuni as judged by ligand-binding blots. These results indicate that the binding of C. jejuni to fibronectin is mediated by the 37 kDa outer membrane protein which is conserved among C. jejuni isolates...
- ChIP on Chip: surprising results are often artifacts
Department of Cell Biology, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo University Hospital and University of Oslo, 0310 Oslo, Norway
BMC Genomics 11:414. 2010
..The method of chromatin immunoprecipitation combined with microarrays (ChIP-Chip) is a powerful tool for genome-wide analysis of protein binding. However, a high background signal is a common phenomenon...
- Acinetobacter baumannii invades epithelial cells and outer membrane protein A mediates interactions with epithelial cells
Chul Hee Choi
Department of Microbiology, Kyungpook National University School of Medicine, Daegu, Korea
BMC Microbiol 8:216. 2008
..This study investigated the potential of A. baumannii to invade epithelial cells and determined the role of A. baumannii outer membrane protein A (AbOmpA) in interactions with epithelial cells...
- Extensive DNA inversions in the B. fragilis genome control variable gene expression
Ana M Cerdeño-Tárraga
Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK
Science 307:1463-5. 2005
..The composition of the 10 different polysaccharide biosynthesis gene clusters identified (7 with associated invertible promoters) suggests a mechanism of synthesis similar to the O-antigen capsules of Escherichia coli...
- Evidence for long-term cervical persistence of Chlamydia trachomatis by omp1 genotyping
Children s Hospital Oakland Research Institute, Oakland, CA 94609, USA
J Infect Dis 182:909-16. 2000
..These data demonstrate that cervical infections with C class serovars can persist for years and may have specific biologic properties that allow for modulation of the major outer membrane protein in response to immune selection...
- Membrane vesicles are immunogenic facsimiles of Salmonella typhimurium that potently activate dendritic cells, prime B and T cell responses, and stimulate protective immunity in vivo
Robert C Alaniz
Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA
J Immunol 179:7692-701. 2007
..Our findings also reveal MVs are a functional nonviable complex vaccine for Salmonella by their ability to prime protective B and T cell responses in vivo...
- A unique mechanism regulating gene expression: translational inhibition by a complementary RNA transcript (micRNA)
Proc Natl Acad Sci U S A 81:1966-70. 1984
..We propose that the micRNA inhibits the translation of the ompF mRNA by hybridizing with it. This RNA interaction may cause premature termination of the transcription of the ompF gene or destabilization of the ompF mRNA or both...
- Biogenesis of beta-barrel membrane proteins in bacteria and eukaryotes: evolutionary conservation and divergence
Dirk M Walther
Interfaculty Institute for Biochemistry, University of Tubingen, Hoppe Seyler Str 4, 72076, Tubingen, Germany
Cell Mol Life Sci 66:2789-804. 2009
..This review summarizes our current knowledge of the functions and biogenesis of this special family of proteins...
- Probing regulon of ArcA in Shewanella oneidensis MR-1 by integrated genomic analyses
Institute for Environmental Genomics and Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 73019, USA
BMC Genomics 9:42. 2008
..Shewanella oneidensis MR-1 contains a gene encoding a putative ArcA homolog with ~81% amino acid sequence identity to the E. coli ArcA protein but not a full-length arcB gene...
- The Bam machine: a molecular cooper
Dante P Ricci
Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
Biochim Biophys Acta 1818:1067-84. 2012
..Special attention will be paid to the Bam machine, a highly conserved multiprotein complex that facilitates OM β-barrel folding. This article is part of a Special Issue entitled: Protein Folding in Membranes...
- Genetic exchange and plasmid transfers in Borrelia burgdorferi sensu stricto revealed by three-way genome comparisons and multilocus sequence typing
Wei Gang Qiu
Department of Biological Sciences, Hunter College of the City University of New York, 695 Park Avenue, New York, NY 10021, USA
Proc Natl Acad Sci U S A 101:14150-5. 2004
..Frequent recombination implies a potential for rapid adaptive evolution and a possible polygenic basis of B. burgdorferi pathogenicity...
- Periplasmic stress and ECF sigma factors
T L Raivio
Department of Biological Sciences, University of Alberta, Edmonton, Alberta, T6G 2E9 Canada
Annu Rev Microbiol 55:591-624. 2001
..Phosphorylated CpxR activates transcription of periplasmic factors necessary for pilus assembly...
- The complement regulator factor H binds to the surface protein OspE of Borrelia burgdorferi
Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Haartmaninkatu 3, FIN-00014 Helsinki, Finland
J Biol Chem 276:8427-35. 2001
..Specific binding of factor H to B. burgdorferi sensu stricto OspE may help the pathogen to evade complement attack and phagocytosis...
- Improved OMV vaccine against Neisseria meningitidis using genetically engineered strains and a detergent-free purification process
Bas van de Waterbeemd
Netherlands Vaccine Institute, Unit Research and Development, The Netherlands
Vaccine 28:4810-6. 2010
..With comparable toxicity and yield, no aggregation and cross-protection against other PorA subtypes, these OMV vaccines are potentially safe and effective for parenteral use in humans...
- Superantigen gene profile diversity among clinical group A streptococcal isolates
Biomedical Laboratory Science, Department of Clinical Microbiology, Umea University, Umea, Sweden
FEMS Immunol Med Microbiol 54:236-44. 2008
..The 22 smeZ alleles were generally linked to the emm-genotype. The results of the investigation show that superantigen gene profiling is useful for tracking spread of clones in the community...
- Recombinant protein meningococcal serogroup B vaccine combined with outer membrane vesicles
Vaccine Evaluation Unit, Health Protection Agency North West, Manchester Medical Microbiology Partnership, Manchester Royal Infirmary, Manchester, M13 9WZ, UK
Expert Opin Biol Ther 11:969-85. 2011
..The novel vaccine Bexsero (previously 4CMenB) has been developed and proven safe and immunogenic in clinical trials...
- Outer membrane vesicles as an acellular vaccine against Acinetobacter baumannii
Michael J McConnell
Unit of Infectious Disease, Microbiology, and Preventive Medicine, Institute of Biomedicine of Sevilla IBIS, University Hospital Virgen del Rocío CSIC University of Sevilla, 41013 Sevilla, Spain
Vaccine 29:5705-10. 2011
..These results indicate that vaccination with outer membrane vesicles may be a viable strategy for preventing A. baumannii infection...
- Single-virus tracking reveals a spatial receptor-dependent search mechanism
Department of Physics, University of Illinois at Urbana Champaign, Urbana, Illinois, USA
Biophys J 100:2875-82. 2011
..We discuss the possible implications of our findings for the process of viral receptor-finding in higher systems...
- Crystal structure of the multidrug exporter MexB from Pseudomonas aeruginosa
Department of Biochemistry, University of Zurich, Switzerland
J Mol Biol 389:134-45. 2009
..As the only missing piece of the puzzle in the MexAB-OprM system, the X-ray structure of MexB completes the molecular picture of the major pump mediating intrinsic and acquired multidrug resistance in P. aeruginosa...
- Secretion of Yop proteins by Yersiniae
Unite de Microbiologie, Universite Catholique de Louvain, Brussels, Belgium
Infect Immun 58:2840-9. 1990
..The release of Yops by Y. enterocolitica occurs by a novel secretion mechanism that does not involve the cleavage of a typical signal sequence or the recognition of a carboxy-terminal domain...
- Immunization with recombinant Brucella species outer membrane protein Omp16 or Omp19 in adjuvant induces specific CD4+ and CD8+ T cells as well as systemic and oral protection against Brucella abortus infection
Karina A Pasquevich
Laboratorio de Inmunogenética, Hospital de Clinicas Jose de San Martin, Facultad de Medicina, Universidad de Buenos Aires, Córdoba 2351 3 Piso Sala 4 1120, Buenos Aires, Argentina
Infect Immun 77:436-45. 2009
..Overall these results indicate that U-Omp16 or U-Omp19 would be a useful candidate for a subunit vaccine against human and animal brucellosis...
- Secretins: dynamic channels for protein transport across membranes
Konstantin V Korotkov
Department of Biochemistry, University of Washington, Seattle, WA, USA
Trends Biochem Sci 36:433-43. 2011
..Secretins might also play a key role in the biogenesis of their cognate secretion systems...
- The outer membrane protein OmpW forms an eight-stranded beta-barrel with a hydrophobic channel
Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908, USA
J Biol Chem 281:7568-77. 2006
..Taken together, the data suggest that members of the OmpW family could be involved in the transport of small hydrophobic molecules across the bacterial outer membrane...
- The crystal structure of BamB suggests interactions with BamA and its role within the BAM complex
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 8030, USA
J Mol Biol 407:248-60. 2011
..This may facilitate integration of newly synthesized OMPs into the outer membrane...
- Evaluation of a high resolution genotyping method for Chlamydia trachomatis using routine clinical samples
Molecular Microbiology and Infection, School of Medicine, University of Southampton, Southampton, United Kingdom
PLoS ONE 6:e16971. 2011
- Structure of the cholera toxin secretion channel in its closed state
Steve L Reichow
Department of Biochemistry, University of Washington, Seattle, Washington, USA
Nat Struct Mol Biol 17:1226-32. 2010
..By combining our results with structures of the cholera toxin and T2SS pseudopilus tip, we provide a structural basis for a possible secretion mechanism of the T2SS...
- Crystal structure of BamD: an essential component of the β-Barrel assembly machinery of gram-negative bacteria
Cristina M Sandoval
Department of Chemistry and Biochemistry, University of Colorado at Boulder, USA
J Mol Biol 409:348-57. 2011
- Plant carbohydrate scavenging through tonB-dependent receptors: a feature shared by phytopathogenic and aquatic bacteria
Laboratoire des Interactions Plantes Microorganismes, Centre National de la Recherche Scientifique CNRS Institut National de la Recherche Agronomique INRA UMR2594 441, Castanet Tolosan, France
PLoS ONE 2:e224. 2007
- Ail binding to fibronectin facilitates Yersinia pestis binding to host cells and Yop delivery
Tiffany M Tsang
Department of Microbiology and Immunology, University of Michigan School of Medicine, 1011 N University, Ann Arbor, MI 48109 1078, USA
Infect Immun 78:3358-68. 2010
..These data indicate that Ail is a key adhesin that mediates binding to host cells through interaction with fibronectin on the surface of host cells, and this interaction is important for Yop delivery by Y. pestis...
- Characterization of MexE-MexF-OprN, a positively regulated multidrug efflux system of Pseudomonas aeruginosa
Department of Genetics and Microbiology, Centre Medical Universitaire, Geneva, Switzerland
Mol Microbiol 23:345-54. 1997
..Evidence is provided to show that the mexEF-oprN operon may be involved in the excretion of intermediates for the biosynthesis of pyocyanin, a typical secondary metabolite of P. aeruginosa...
- First report and detailed characterization of B. pertussis isolates not expressing Pertussis Toxin or Pertactin
Institut Pasteur, Unité Prévention et Thérapie Moléculaires des Maladies Humaines, URA CNRS 3012, 25 rue du Dr Roux, 75015 Paris, France
Vaccine 27:6034-41. 2009
..All isolates were found less pathogen in animal or cellular models; their circulation raises the problem of clinical and biological diagnoses...
- Pertactin is required for Bordetella species to resist neutrophil-mediated clearance
Carol S Inatsuka
Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, Santa Barbara, California 93106 9610, USA
Infect Immun 78:2901-9. 2010
- Modulation of Neisseria gonorrhoeae susceptibility to vertebrate antibacterial peptides due to a member of the resistance/nodulation/division efflux pump family
W M Shafer
Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322, USA
Proc Natl Acad Sci U S A 95:1829-33. 1998
..These results indicate that such efflux systems may enable mucosal pathogens like gonococci to resist endogenous antimicrobial peptides that are thought to act during infection...
- Probing the ArcA-P modulon of Escherichia coli by whole genome transcriptional analysis and sequence recognition profiling
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
J Biol Chem 279:12588-97. 2004
..A conservative but reasonable assessment is that the Arc pathway recruits 100-150 operons to mediate a role in cellular adaptation that is more extensive than hitherto anticipated...
- M protein, a classical bacterial virulence determinant, forms complexes with fibrinogen that induce vascular leakage
Department of Cell and Molecular Biology, Lund University, Tornavagen 10, S 221 84 Lund, Sweden
Cell 116:367-79. 2004
..In addition, M protein/fibrinogen complexes were identified in tissue biopsies from a patient with necrotizing fasciitis and STSS, further underlining the pathogenic significance of such complexes in severe streptococcal infections...
- Antigenic and genetic heterogeneity of Borrelia burgdorferi populations transmitted by ticks
Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, NC 27599, USA
Proc Natl Acad Sci U S A 98:670-5. 2001
..burgdorferi transmission. Implicit in our model is the concept that tick transmission converts a homogeneous spirochete population into a heterogeneous population that is poised to infect the mammalian host...
- Properties and clinical performance of vaccines containing outer membrane vesicles from Neisseria meningitidis
Department of Bacteriology and Immunology, Norwegian Institute of Public Health, Oslo, P O Box 4404 Nydalen, Oslo NO 0403, Norway
Vaccine 27:B3-12. 2009
..The desire to have a global vaccine strategy that enables susceptible individuals to be protected against all the relevant serogroups of meningococcal disease may become a reality...
- Crystal structures of various maltooligosaccharides bound to maltoporin reveal a specific sugar translocation pathway
Department of Structural Biology, Biozentrum, University of Basel, CH 4056 Basel, Switzerland
Structure 4:127-34. 1996
..coli. In particular, it is indispensable for the transport of long maltooligosaccharides, as these do not pass through non-specific porins. An understanding of this intriguing capability requires elucidation of the structural basis...
- Overexpression of the mexC-mexD-oprJ efflux operon in nfxB-type multidrug-resistant strains of Pseudomonas aeruginosa
Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
Mol Microbiol 21:713-24. 1996
..These data indicate that the multidrug resistance of nfxB strains is due to overexpression of an efflux operon, mexC-mexD-oprJ, encoding components of a second efflux pump in P. aeruginosa...