bacterial outer membrane proteins

Summary

Summary: Proteins isolated from the outer membrane of Gram-negative bacteria.

Top Publications

  1. ncbi Multidrug-resistance efflux pumps - not just for resistance
    Laura J V Piddock
    Antimicrobial Agents Research Group, Division of Immunity and Infection, The Medical School, University of Birmingham, Birmingham B15 2TT, UK
    Nat Rev Microbiol 4:629-36. 2006
  2. pmc Coupled degradation of a small regulatory RNA and its mRNA targets in Escherichia coli
    Eric Massé
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genes Dev 17:2374-83. 2003
  3. ncbi A genomic view of the human-Bacteroides thetaiotaomicron symbiosis
    Jian Xu
    Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St Louis, MO 63110, USA
    Science 299:2074-6. 2003
  4. pmc The stringent response and cell cycle arrest in Escherichia coli
    Daniel J Ferullo
    Department of Biology and Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA, USA
    PLoS Genet 4:e1000300. 2008
  5. ncbi Crystal structures of a multidrug transporter reveal a functionally rotating mechanism
    Satoshi Murakami
    Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567 0047, Japan
    Nature 443:173-9. 2006
  6. ncbi The Lyme disease agent exploits a tick protein to infect the mammalian host
    Nandhini Ramamoorthi
    Sections of Rheumatology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    Nature 436:573-7. 2005
  7. ncbi TROSPA, an Ixodes scapularis receptor for Borrelia burgdorferi
    Utpal Pal
    Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520, USA
    Cell 119:457-68. 2004
  8. pmc The Acinetobacter baumannii 19606 OmpA protein plays a role in biofilm formation on abiotic surfaces and in the interaction of this pathogen with eukaryotic cells
    Jennifer A Gaddy
    Department of Microbiology, Miami University, Oxford, OH 45056, USA
    Infect Immun 77:3150-60. 2009
  9. pmc Outer-surface protein C of the Lyme disease spirochete: a protein induced in ticks for infection of mammals
    Dorothee Grimm
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South Fourth Street, Hamilton, MT 59840, USA
    Proc Natl Acad Sci U S A 101:3142-7. 2004
  10. ncbi Structure and function of bacterial outer membrane proteins: barrels in a nutshell
    R Koebnik
    Biozentrum Basel, Abteilung Mikrobiologie, Klingelbergstr 50, CH 4056 Basel, Switzerland
    Mol Microbiol 37:239-53. 2000

Detail Information

Publications325 found, 100 shown here

  1. ncbi Multidrug-resistance efflux pumps - not just for resistance
    Laura J V Piddock
    Antimicrobial Agents Research Group, Division of Immunity and Infection, The Medical School, University of Birmingham, Birmingham B15 2TT, UK
    Nat Rev Microbiol 4:629-36. 2006
    ..Here, I present the accumulating evidence that multidrug-resistance efflux pumps have roles in bacterial pathogenicity and propose that these pumps therefore have greater clinical relevance than is usually attributed to them...
  2. pmc Coupled degradation of a small regulatory RNA and its mRNA targets in Escherichia coli
    Eric Massé
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genes Dev 17:2374-83. 2003
    ..Thus, this large class of regulatory RNAs share an unexpected intrinsic mechanism for shutting off their action...
  3. ncbi A genomic view of the human-Bacteroides thetaiotaomicron symbiosis
    Jian Xu
    Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St Louis, MO 63110, USA
    Science 299:2074-6. 2003
    ..These and other expanded paralogous groups shed light on the molecular mechanisms underlying symbiotic host-bacterial relationships in our intestine...
  4. pmc The stringent response and cell cycle arrest in Escherichia coli
    Daniel J Ferullo
    Department of Biology and Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA, USA
    PLoS Genet 4:e1000300. 2008
    ..This is the first indication that bacterial chromosome segregation, whose mechanism is not understood, is a step that may be regulated in response to environmental conditions...
  5. ncbi Crystal structures of a multidrug transporter reveal a functionally rotating mechanism
    Satoshi Murakami
    Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567 0047, Japan
    Nature 443:173-9. 2006
    ..The voluminous binding pocket is aromatic and allows multi-site binding. The structures indicate that drugs are exported by a three-step functionally rotating mechanism in which substrates undergo ordered binding change...
  6. ncbi The Lyme disease agent exploits a tick protein to infect the mammalian host
    Nandhini Ramamoorthi
    Sections of Rheumatology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    Nature 436:573-7. 2005
    ..scapularis drastically reduced the capacity of tick-borne spirochaetes to infect mice. These results show the capacity of a pathogen to use a secreted arthropod protein to help it colonize the mammalian host...
  7. ncbi TROSPA, an Ixodes scapularis receptor for Borrelia burgdorferi
    Utpal Pal
    Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520, USA
    Cell 119:457-68. 2004
    ..Identification of an I. scapularis receptor for B. burgdorferi is the first step toward elucidating arthropod ligands that are required for survival of spirochetes in nature...
  8. pmc The Acinetobacter baumannii 19606 OmpA protein plays a role in biofilm formation on abiotic surfaces and in the interaction of this pathogen with eukaryotic cells
    Jennifer A Gaddy
    Department of Microbiology, Miami University, Oxford, OH 45056, USA
    Infect Immun 77:3150-60. 2009
    ..Interestingly, the killing activity of cell-free culture supernatants proved to be protease and temperature sensitive, suggesting that its cytotoxic activity is due to secreted proteins, some of which are different from OmpA...
  9. pmc Outer-surface protein C of the Lyme disease spirochete: a protein induced in ticks for infection of mammals
    Dorothee Grimm
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South Fourth Street, Hamilton, MT 59840, USA
    Proc Natl Acad Sci U S A 101:3142-7. 2004
    ..The induction of a spirochetal virulence factor preceding the time and host in which it is required demonstrates a developmental sequence for transmission of this arthropod-borne pathogen...
  10. ncbi Structure and function of bacterial outer membrane proteins: barrels in a nutshell
    R Koebnik
    Biozentrum Basel, Abteilung Mikrobiologie, Klingelbergstr 50, CH 4056 Basel, Switzerland
    Mol Microbiol 37:239-53. 2000
    ..Our review is aimed at discussing their common principles and peculiarities as well as open questions associated with them...
  11. pmc Release of outer membrane vesicles by Gram-negative bacteria is a novel envelope stress response
    Amanda J McBroom
    Duke University Medical Center, Department of Biochemistry Box 3711, Durham, NC 27710, USA
    Mol Microbiol 63:545-58. 2007
    ..In addition to identifying a novel mechanism for alleviating stress, this work provides physiological relevance for vesicle production as a protective mechanism...
  12. ncbi Membrane protein architects: the role of the BAM complex in outer membrane protein assembly
    Timothy J Knowles
    Cancer Research UK Institute for Cancer Studies, School of Cancer Sciences, Edgbaston, Birmingham, UK
    Nat Rev Microbiol 7:206-14. 2009
    ..Here, we discuss how these structures illuminate the mechanisms responsible for the biogenesis of outer membrane proteins...
  13. ncbi Complexity and complementarity of outer membrane protein A recognition by cellular and humoral innate immunity receptors
    Pascale Jeannin
    Centre d Immunologie Pierre Fabre, 5 Avenue Napoleon III, 74164 Saint Julien en Genevois, France
    Immunity 22:551-60. 2005
    ..Thus, SR-mediated KpOmpA recognition and TLR2-dependent cellular activation set in motion a nonredundant PTX3-mediated humoral amplification loop of innate immunity...
  14. ncbi Crystal structure of bacterial multidrug efflux transporter AcrB
    Satoshi Murakami
    Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567 0047, Japan
    Nature 419:587-93. 2002
    ....
  15. pmc Structures of gram-negative cell walls and their derived membrane vesicles
    T J Beveridge
    Canadian Bacterial Disease Network, and Department of Microbiology, College of Biological Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1
    J Bacteriol 181:4725-33. 1999
  16. ncbi Outer membrane protein 38 of Acinetobacter baumannii localizes to the mitochondria and induces apoptosis of epithelial cells
    Chul Hee Choi
    Department of Microbiology, Kyungpook National University School of Medicine, Daegu 700 422, Korea
    Cell Microbiol 7:1127-38. 2005
    ..These results demonstrate that Omp38 may act as a potential virulence factor to induce apoptosis of epithelial cells in the early stage of A. baumannii infection...
  17. pmc Cloning and characterization of a gene encoding the major surface protein of the bacterial endosymbiont Wolbachia pipientis
    H R Braig
    Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Bacteriol 180:2373-8. 1998
    ..As such, this gene will be a very useful tool for Wolbachia strain typing and phylogenetic analysis, as well as understanding the molecular basis of the interaction of Wolbachia with its host...
  18. pmc SigmaE-dependent small RNAs of Salmonella respond to membrane stress by accelerating global omp mRNA decay
    Kai Papenfort
    Max Planck Institute for Infection Biology, Chariteplatz 1, 10117 Berlin, Germany
    Mol Microbiol 62:1674-88. 2006
    ..In unstressed bacterial cells, the two oE-dependent small RNAs function within a surveillance loop to maintain envelope homeostasis and to achieve autoregulation of oE...
  19. pmc SciN is an outer membrane lipoprotein required for type VI secretion in enteroaggregative Escherichia coli
    Marie Stéphanie Aschtgen
    Laboratoire d Ingenierie des Systemes Macromoleculaires, Institut de Biologie Structurale et Microbiologie, CNRS, UPR 9027, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    J Bacteriol 190:7523-31. 2008
    ..Together, these results support a model in which SciN is an outer membrane lipoprotein exposed in the periplasm and essential for the Sci-1 apparatus function...
  20. pmc The meningococcal vaccine candidate GNA1870 binds the complement regulatory protein factor H and enhances serum resistance
    Guillermo Madico
    Section of Infectious Diseases, Boston University School of Medicine, Boston, MA 02118, USA
    J Immunol 177:501-10. 2006
    ..These data provide further support for inclusion of this molecule in a meningococcal vaccine. To reflect the critical function of this molecule, we suggest calling it fH-binding protein...
  21. pmc Prevalence and genetic characterization of pertactin-deficient Bordetella pertussis in Japan
    Nao Otsuka
    Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan
    PLoS ONE 7:e31985. 2012
    ..Our observations suggested that human host factors (genetic factors and immune status) that select for Prn(-) strains have arisen and that Prn expression is not essential for fitness under these conditions...
  22. ncbi β-Barrel membrane protein assembly by the Bam complex
    Christine L Hagan
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Annu Rev Biochem 80:189-210. 2011
    ....
  23. pmc OspC is potent plasminogen receptor on surface of Borrelia burgdorferi
    Ozlem Onder
    Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 287:16860-8. 2012
    ..The co-localization of plasminogen and OspC on OspC-expressing spirochetes further implicates OspC as a biologically relevant plasminogen receptor on the surface of live B. burgdorferi...
  24. pmc Hfq-dependent regulation of OmpA synthesis is mediated by an antisense RNA
    Klas I Udekwu
    Department of Cell and Molecular Biology, Uppsala University, S 75124 Uppsala, Sweden
    Genes Dev 19:2355-66. 2005
    ..MicA requires the presence of the Hfq protein, although the mechanistic basis for this remains unclear...
  25. ncbi Identification of a multicomponent complex required for outer membrane biogenesis in Escherichia coli
    Tao Wu
    Department of Chemistry, Princeton University, Princeton, New Jersey 08544, USA
    Cell 121:235-45. 2005
    ..These genetic interactions suggest a role for YfgL, one of the lipoprotein components of the protein assembly complex, in a homeostatic control mechanism that coordinates the overall OM assembly process...
  26. ncbi A phase 1 study of a meningococcal native outer membrane vesicle vaccine made from a group B strain with deleted lpxL1 and synX, over-expressed factor H binding protein, two PorAs and stabilized OpcA expression
    P B Keiser
    Walter Reed Army Institute of Research WRAIR, Silver Spring, MD, USA
    Vaccine 29:1413-20. 2011
    ..Depletion assays show contributions to bactericidal activity from antibodies to lipooligosaccharide (LOS), fHbp v.1 and OpcA...
  27. pmc Immunological and molecular polymorphisms of OspC, an immunodominant major outer surface protein of Borrelia burgdorferi
    B Wilske
    Max von Pettenkofer Institut fur Hygiene und Medizinische Mikrobiologie, Universitat Munchen, Germany
    Infect Immun 61:2182-91. 1993
    ....
  28. ncbi Outer membrane proteins: key players for bacterial adaptation in host niches
    Jun Lin
    Food Animal Health Research Program, Department of Veterinary Preventive Medicine, The Ohio State University, 1680 Madison Avenue, Wooster, OH 44691, USA
    Microbes Infect 4:325-31. 2002
    ..In this paper, we will present a brief review on OMPs that contribute to bacterial adaptive responses including iron uptake, antimicrobial peptide resistance, serum resistance, and drug/bile resistance...
  29. pmc Associations between virulence factors of Shiga toxin-producing Escherichia coli and disease in humans
    P Boerlin
    Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada
    J Clin Microbiol 37:497-503. 1999
    ....
  30. pmc Multiplex real-time PCR for detection of anaplasma phagocytophilum and Borrelia burgdorferi
    Joshua W Courtney
    Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd, MS G 13, Atlanta, GA 30333, USA
    J Clin Microbiol 42:3164-8. 2004
    ..phagocytophilum and Borrelia species, two of the most common tick-borne infectious agents in the United States...
  31. pmc Outer membrane permeability and antibiotic resistance
    Anne H Delcour
    Department of Biology, University of Houston, 369 Science and Research Building II, Houston, TX 77204 5001, USA
    Biochim Biophys Acta 1794:808-16. 2009
    ..This review will describe the molecular mechanisms for permeation of antibiotics through the outer membrane, and the strategies that bacteria have deployed to resist antibiotics by modifications of these pathways...
  32. pmc Borrelia burgdorferi OspC protein required exclusively in a crucial early stage of mammalian infection
    Kit Tilly
    Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, MT 59840, USA
    Infect Immun 74:3554-64. 2006
    ..We conclude that OspC is indispensable for establishing infection by B. burgdorferi in mammals but is not required at any other point of the mouse-tick infection cycle...
  33. ncbi The trans-envelope Tol-Pal complex is part of the cell division machinery and required for proper outer-membrane invagination during cell constriction in E. coli
    Matthew A Gerding
    Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, OH, USA
    Mol Microbiol 63:1008-25. 2007
    ....
  34. pmc Rickettsioses as paradigms of new or emerging infectious diseases
    D Raoult
    Unite des Rickettsies, Faculte de Medecine, CNRS UPRESA 6020, Marseille, France
    Clin Microbiol Rev 10:694-719. 1997
    ..We also assess the pathogenic potential of rickettsial strains that to date have been associated only with arthropods, and we discuss diseases of unknown etiology that may be rickettsioses...
  35. ncbi Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis
    R S Stephens
    Program in Infectious Diseases, University of California, Berkeley, CA 94720, USA
    Science 282:754-9. 1998
    ..The phylogenetic mosaic of chlamydial genes, including a large number of genes with phylogenetic origins from eukaryotes, implies a complex evolution for adaptation to obligate intracellular parasitism...
  36. ncbi Global emm type distribution of group A streptococci: systematic review and implications for vaccine development
    Andrew C Steer
    Centre for International Child Health, University of Melbourne, Australia
    Lancet Infect Dis 9:611-6. 2009
    ....
  37. pmc HHomp--prediction and classification of outer membrane proteins
    Michael Remmert
    Department of Protein Evolution, Max Planck Institute for Developmental Biology, Spemannstrasse 35, 72076 Tubingen, Germany
    Nucleic Acids Res 37:W446-51. 2009
    ..In Escherichia coli, HHomp identifies 57 out of 59 known OMPs and correctly assigns them to their functional subgroups. HHomp can be accessed at http://toolkit.tuebingen.mpg.de/hhomp...
  38. ncbi Structure of a type IV secretion system core complex
    Remi Fronzes
    Institute of Structural and Molecular Biology, School of Crystallography, Birkbeck College, Malet Street, London, WC1E 7HX, UK
    Science 323:266-8. 2009
    ..Overall, the T4SS core complex structure is different in both architecture and composition from the other known double membrane-spanning secretion system that has been structurally characterized...
  39. ncbi Next-generation outer membrane vesicle vaccines against Neisseria meningitidis based on nontoxic LPS mutants
    Peter van der Ley
    Unit Vaccinology, National Institute of Public Health and the Environment RIVM, Bilthoven, The Netherlands
    Hum Vaccin 7:886-90. 2011
    ..Several groups have now started the development of native OMV vaccines based on non-toxic LPS mutants, and this Commentary provides an overview of the various approaches and results thus far...
  40. ncbi Actinobacillus pleuropneumoniae: pathobiology and pathogenesis of infection
    Janine T Bosse
    Molecular Infectious Diseases Group, Department of Paediatrics, Faculty of Medicine, Imperial College, St Mary s Campus, W2 1PG, London, UK
    Microbes Infect 4:225-35. 2002
    ....
  41. ncbi Tolerance to the antimicrobial peptide colistin in Pseudomonas aeruginosa biofilms is linked to metabolically active cells, and depends on the pmr and mexAB-oprM genes
    Sünje Johanna Pamp
    BioCentrum DTU, Technical University of Denmark, Lyngby, Denmark
    Mol Microbiol 68:223-40. 2008
    ..Consequently, targeting the two physiologically distinct subpopulations by combined antimicrobial treatment with either ciprofloxacin and colistin or tetracycline and colistin almost completely eradicated all biofilm cells...
  42. pmc Transcript profiling and inference of Escherichia coli K-12 ArcA activity across the range of physiologically relevant oxygen concentrations
    Matthew D Rolfe
    The Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, United Kingdom
    J Biol Chem 286:10147-54. 2011
    ....
  43. pmc Relationship between immunity to Borrelia burgdorferi outer-surface protein A (OspA) and Lyme arthritis
    Allen C Steere
    Center for Immunology and Inflammatory Diseases, Massachusetts General Hospital, Harvard Medical School, Boston, USA
    Clin Infect Dis 52:s259-65. 2011
    ..Thus, a vaccine-induced immune response to OspA does not replicate the sequence of events needed in the natural infection to induce antibiotic-refractory Lyme arthritis...
  44. ncbi A molecular Swiss army knife: OmpA structure, function and expression
    Stephen G J Smith
    Department of Clinical Microbiology, Trinity College Dublin, St James s Hospital, Dublin, Ireland
    FEMS Microbiol Lett 273:1-11. 2007
    ..Many of these properties are due to four short protein loops that emanate from the protein to the outside of the cell. Herein it is described how the structure of this protein relates to its many functions...
  45. ncbi SoxS regulates the expression of the Salmonella enterica serovar Typhimurium ompW gene
    F Gil
    Laboratorio de Microbiologia Molecular, Departamento de Ciencias Biologicas, Universidad Andres Bello, Santiago, Chile
    Microbiology 155:2490-7. 2009
    ..Transcriptional fusions support the relevance of this region in ompW activation. The SoxS site is in the forward orientation and its location suggests that the ompW gene has a class I SoxS-dependent promoter...
  46. pmc Vaccination with outer membrane complexes elicits rapid protective immunity to multidrug-resistant Acinetobacter baumannii
    Michael J McConnell
    Unit of Infectious Disease, Microbiology, and Preventive Medicine, Hospitales Universitarios Virgen del Rocio, Edificio Laboratorio Anexo Caracola, Avenida Manuel Siurot s n, 41013 Seville, Spain
    Infect Immun 79:518-26. 2011
    ..These results indicate that prophylactic vaccination and antibody-based therapies based on an outer membrane complex vaccine may be viable approaches to preventing the morbidity and mortality caused by this pathogen...
  47. pmc Identification of a protein complex that assembles lipopolysaccharide in the outer membrane of Escherichia coli
    Tao Wu
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 103:11754-9. 2006
    ..We show that RlpB is also essential for cell viability and that the Imp/RlpB complex is responsible for LPS reaching the outer surface of the outer membrane...
  48. pmc Evaluation of high-resolution typing methods for Chlamydia trachomatis in samples from heterosexual couples
    Reinier J M Bom
    Public Health Laboratory, Public Health Service of Amsterdam, Nieuwe Achtergracht 100, 1018 WT Amsterdam, The Netherlands
    J Clin Microbiol 49:2844-53. 2011
    ..This shows that the chlamydial genome in clinical specimens has an appropriate polymorphism to enable epidemiological cluster analysis using MLST and MLVA...
  49. pmc Identification and characterization of inhibitors of multidrug resistance efflux pumps in Pseudomonas aeruginosa: novel agents for combination therapy
    O Lomovskaya
    Microcide Pharmaceuticals, Inc, Mountain View, California 94043, USA
    Antimicrob Agents Chemother 45:105-16. 2001
    ..aeruginosa strains that are highly resistant to fluoroquinolones...
  50. pmc Brucella abortus induces the secretion of proinflammatory mediators from glial cells leading to astrocyte apoptosis
    Clara García Samartino
    Instituto de Estudios de la Inmunidad Humoral IDEHU, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires Junín 956 4 Piso 1113 Buenos Aires, Argentina
    Am J Pathol 176:1323-38. 2010
    ..Our results provide proof of the principle that Brucella lipoproteins could be key virulence factors in neurobrucellosis and that astrogliosis might contribute to neurobrucellosis pathogenesis...
  51. ncbi Crystal structure of the bacterial membrane protein TolC central to multidrug efflux and protein export
    V Koronakis
    Department of Pathology, University of Cambridge, UK
    Nature 405:914-9. 2000
    ..The structure provides an explanation of how the cell cytosol is connected to the external environment during export, and suggests a general mechanism for the action of bacterial efflux pumps...
  52. ncbi Structural basis for the regulated protease and chaperone function of DegP
    Tobias Krojer
    Research Institute for Molecular Pathology IMP, Dr Bohrgasse 7, A 1030 Vienna, Austria
    Nature 453:885-90. 2008
    ..Oligomer reassembly and concomitant activation on substrate binding may also be critical in regulating other HtrA proteases implicated in protein-folding diseases...
  53. pmc Geographic uniformity of the Lyme disease spirochete (Borrelia burgdorferi) and its shared history with tick vector (Ixodes scapularis) in the Northeastern United States
    Wei Gang Qiu
    Department of Ecology and Evolution, State University of New York, Stony Brook, New York 11794 5245, USA
    Genetics 160:833-49. 2002
    ..We therefore conclude that the genetic structure of B. burgdorferi has been intimately shaped by the natural history of its main vector, the northern lineage of I. scapularis ticks...
  54. pmc Expression of Borrelia burgdorferi OspC and DbpA is controlled by a RpoN-RpoS regulatory pathway
    A Hubner
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 98:12724-9. 2001
    ..burgdorferi. Our findings provide a foundation for elucidating further key regulatory networks that potentially impact many aspects of B. burgdorferi's parasitic strategy, host range, and virulence expression...
  55. pmc A program of Yersinia enterocolitica type III secretion reactions is activated by specific signals
    V T Lee
    Department of Microbiology and Immunology, University of California Los Angeles School of Medicine, Los Angeles, California 90095, USA
    J Bacteriol 183:4970-8. 2001
    ..Together these results suggest that yersiniae may have evolved distinct secretion reactions in response to environmental signals...
  56. pmc Requirement of ArcA for redox regulation in Escherichia coli under microaerobic but not anaerobic or aerobic conditions
    Svetlana Alexeeva
    Laboratory for Microbiology, Swammerdam Institute for Life Sciences, University of Amsterdam, Nieuwe Achtergracht 166, 1018WV Amsterdam, The Netherlands
    J Bacteriol 185:204-9. 2003
    ..Thus, the ArcA regulator was found to exert major control on flux distribution, and it is concluded that the ArcAB system should be considered a microaerobic redox regulator...
  57. pmc A Pseudomonas syringae type III effector suppresses cell wall-based extracellular defense in susceptible Arabidopsis plants
    Paula Hauck
    Department of Energy Plant Research Laboratory and Department of Plant Biology, Michigan State University, East Lansing, MI 48824, USA
    Proc Natl Acad Sci U S A 100:8577-82. 2003
    ..AvrPto is therefore one of the long postulated suppressors of an salicylic acid-independent, cell wall-based defense that is aimed at hrp mutant bacteria...
  58. pmc Electrically conductive bacterial nanowires produced by Shewanella oneidensis strain MR-1 and other microorganisms
    Yuri A Gorby
    Pacific Northwest National Laboratory, Richland, WA 99352, USA
    Proc Natl Acad Sci U S A 103:11358-63. 2006
    ....
  59. ncbi Acinetobacter baumannii outer membrane protein A targets the nucleus and induces cytotoxicity
    Chul Hee Choi
    Department of Microbiology, Kyungpook National University School of Medicine, Daegu, Korea
    Cell Microbiol 10:309-19. 2008
    ..We propose a novel pathogenic mechanism of A. baumannii regarding the nuclear targeting of the bacterial structural protein AbOmpA...
  60. ncbi Proteomics in gram-negative bacterial outer membrane vesicles
    Eun Young Lee
    Department of Life Science and Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea
    Mass Spectrom Rev 27:535-55. 2008
    ....
  61. pmc The ArcBA two-component system of Escherichia coli is regulated by the redox state of both the ubiquinone and the menaquinone pool
    Martijn Bekker
    Swammerdam Institute for Life Sciences Molecular Microbial Physiology, University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV Amsterdam, The Netherlands
    J Bacteriol 192:746-54. 2010
    ..coli is modulated by the redox state of the menaquinone pool and that the ubiquinone/ubiquinol ratio in vivo surely is not the only determinant of ArcB activity...
  62. pmc An ecological approach to preventing human infection: vaccinating wild mouse reservoirs intervenes in the Lyme disease cycle
    Jean I Tsao
    Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, CT 06520, USA
    Proc Natl Acad Sci U S A 101:18159-64. 2004
    ..Thus, where nonmouse hosts play a large role in infection dynamics, vaccination should be directed at additional species...
  63. ncbi Identification and molecular cloning of a gene encoding a fibronectin-binding protein (CadF) from Campylobacter jejuni
    M E Konkel
    Department of Microbiology, Washington State University, Pullman 99164 4233, USA
    Mol Microbiol 24:953-63. 1997
    ..jejuni as judged by ligand-binding blots. These results indicate that the binding of C. jejuni to fibronectin is mediated by the 37 kDa outer membrane protein which is conserved among C. jejuni isolates...
  64. pmc ChIP on Chip: surprising results are often artifacts
    Torsten Waldminghaus
    Department of Cell Biology, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo University Hospital and University of Oslo, 0310 Oslo, Norway
    BMC Genomics 11:414. 2010
    ..The method of chromatin immunoprecipitation combined with microarrays (ChIP-Chip) is a powerful tool for genome-wide analysis of protein binding. However, a high background signal is a common phenomenon...
  65. pmc Acinetobacter baumannii invades epithelial cells and outer membrane protein A mediates interactions with epithelial cells
    Chul Hee Choi
    Department of Microbiology, Kyungpook National University School of Medicine, Daegu, Korea
    BMC Microbiol 8:216. 2008
    ..This study investigated the potential of A. baumannii to invade epithelial cells and determined the role of A. baumannii outer membrane protein A (AbOmpA) in interactions with epithelial cells...
  66. ncbi Extensive DNA inversions in the B. fragilis genome control variable gene expression
    Ana M Cerdeño-Tárraga
    Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK
    Science 307:1463-5. 2005
    ..The composition of the 10 different polysaccharide biosynthesis gene clusters identified (7 with associated invertible promoters) suggests a mechanism of synthesis similar to the O-antigen capsules of Escherichia coli...
  67. ncbi Evidence for long-term cervical persistence of Chlamydia trachomatis by omp1 genotyping
    D Dean
    Children s Hospital Oakland Research Institute, Oakland, CA 94609, USA
    J Infect Dis 182:909-16. 2000
    ..These data demonstrate that cervical infections with C class serovars can persist for years and may have specific biologic properties that allow for modulation of the major outer membrane protein in response to immune selection...
  68. ncbi Membrane vesicles are immunogenic facsimiles of Salmonella typhimurium that potently activate dendritic cells, prime B and T cell responses, and stimulate protective immunity in vivo
    Robert C Alaniz
    Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA
    J Immunol 179:7692-701. 2007
    ..Our findings also reveal MVs are a functional nonviable complex vaccine for Salmonella by their ability to prime protective B and T cell responses in vivo...
  69. pmc A unique mechanism regulating gene expression: translational inhibition by a complementary RNA transcript (micRNA)
    T Mizuno
    Proc Natl Acad Sci U S A 81:1966-70. 1984
    ..We propose that the micRNA inhibits the translation of the ompF mRNA by hybridizing with it. This RNA interaction may cause premature termination of the transcription of the ompF gene or destabilization of the ompF mRNA or both...
  70. pmc Biogenesis of beta-barrel membrane proteins in bacteria and eukaryotes: evolutionary conservation and divergence
    Dirk M Walther
    Interfaculty Institute for Biochemistry, University of Tubingen, Hoppe Seyler Str 4, 72076, Tubingen, Germany
    Cell Mol Life Sci 66:2789-804. 2009
    ..This review summarizes our current knowledge of the functions and biogenesis of this special family of proteins...
  71. pmc Probing regulon of ArcA in Shewanella oneidensis MR-1 by integrated genomic analyses
    Haichun Gao
    Institute for Environmental Genomics and Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 73019, USA
    BMC Genomics 9:42. 2008
    ..Shewanella oneidensis MR-1 contains a gene encoding a putative ArcA homolog with ~81% amino acid sequence identity to the E. coli ArcA protein but not a full-length arcB gene...
  72. pmc The Bam machine: a molecular cooper
    Dante P Ricci
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Biochim Biophys Acta 1818:1067-84. 2012
    ..Special attention will be paid to the Bam machine, a highly conserved multiprotein complex that facilitates OM β-barrel folding. This article is part of a Special Issue entitled: Protein Folding in Membranes...
  73. pmc Genetic exchange and plasmid transfers in Borrelia burgdorferi sensu stricto revealed by three-way genome comparisons and multilocus sequence typing
    Wei Gang Qiu
    Department of Biological Sciences, Hunter College of the City University of New York, 695 Park Avenue, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 101:14150-5. 2004
    ..Frequent recombination implies a potential for rapid adaptive evolution and a possible polygenic basis of B. burgdorferi pathogenicity...
  74. ncbi Periplasmic stress and ECF sigma factors
    T L Raivio
    Department of Biological Sciences, University of Alberta, Edmonton, Alberta, T6G 2E9 Canada
    Annu Rev Microbiol 55:591-624. 2001
    ..Phosphorylated CpxR activates transcription of periplasmic factors necessary for pilus assembly...
  75. ncbi The complement regulator factor H binds to the surface protein OspE of Borrelia burgdorferi
    J Hellwage
    Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Haartmaninkatu 3, FIN-00014 Helsinki, Finland
    J Biol Chem 276:8427-35. 2001
    ..Specific binding of factor H to B. burgdorferi sensu stricto OspE may help the pathogen to evade complement attack and phagocytosis...
  76. ncbi Improved OMV vaccine against Neisseria meningitidis using genetically engineered strains and a detergent-free purification process
    Bas van de Waterbeemd
    Netherlands Vaccine Institute, Unit Research and Development, The Netherlands
    Vaccine 28:4810-6. 2010
    ..With comparable toxicity and yield, no aggregation and cross-protection against other PorA subtypes, these OMV vaccines are potentially safe and effective for parenteral use in humans...
  77. ncbi Superantigen gene profile diversity among clinical group A streptococcal isolates
    Linda Maripuu
    Biomedical Laboratory Science, Department of Clinical Microbiology, Umea University, Umea, Sweden
    FEMS Immunol Med Microbiol 54:236-44. 2008
    ..The 22 smeZ alleles were generally linked to the emm-genotype. The results of the investigation show that superantigen gene profiling is useful for tracking spread of clones in the community...
  78. ncbi Recombinant protein meningococcal serogroup B vaccine combined with outer membrane vesicles
    Xilian Bai
    Vaccine Evaluation Unit, Health Protection Agency North West, Manchester Medical Microbiology Partnership, Manchester Royal Infirmary, Manchester, M13 9WZ, UK
    Expert Opin Biol Ther 11:969-85. 2011
    ..The novel vaccine Bexsero (previously 4CMenB) has been developed and proven safe and immunogenic in clinical trials...
  79. ncbi Outer membrane vesicles as an acellular vaccine against Acinetobacter baumannii
    Michael J McConnell
    Unit of Infectious Disease, Microbiology, and Preventive Medicine, Institute of Biomedicine of Sevilla IBIS, University Hospital Virgen del Rocío CSIC University of Sevilla, 41013 Sevilla, Spain
    Vaccine 29:5705-10. 2011
    ..These results indicate that vaccination with outer membrane vesicles may be a viable strategy for preventing A. baumannii infection...
  80. pmc Single-virus tracking reveals a spatial receptor-dependent search mechanism
    Eli Rothenberg
    Department of Physics, University of Illinois at Urbana Champaign, Urbana, Illinois, USA
    Biophys J 100:2875-82. 2011
    ..We discuss the possible implications of our findings for the process of viral receptor-finding in higher systems...
  81. ncbi Crystal structure of the multidrug exporter MexB from Pseudomonas aeruginosa
    Gaby Sennhauser
    Department of Biochemistry, University of Zurich, Switzerland
    J Mol Biol 389:134-45. 2009
    ..As the only missing piece of the puzzle in the MexAB-OprM system, the X-ray structure of MexB completes the molecular picture of the major pump mediating intrinsic and acquired multidrug resistance in P. aeruginosa...
  82. pmc Secretion of Yop proteins by Yersiniae
    T Michiels
    Unite de Microbiologie, Universite Catholique de Louvain, Brussels, Belgium
    Infect Immun 58:2840-9. 1990
    ..The release of Yops by Y. enterocolitica occurs by a novel secretion mechanism that does not involve the cleavage of a typical signal sequence or the recognition of a carboxy-terminal domain...
  83. pmc Immunization with recombinant Brucella species outer membrane protein Omp16 or Omp19 in adjuvant induces specific CD4+ and CD8+ T cells as well as systemic and oral protection against Brucella abortus infection
    Karina A Pasquevich
    Laboratorio de Inmunogenética, Hospital de Clinicas Jose de San Martin, Facultad de Medicina, Universidad de Buenos Aires, Córdoba 2351 3 Piso Sala 4 1120, Buenos Aires, Argentina
    Infect Immun 77:436-45. 2009
    ..Overall these results indicate that U-Omp16 or U-Omp19 would be a useful candidate for a subunit vaccine against human and animal brucellosis...
  84. pmc Secretins: dynamic channels for protein transport across membranes
    Konstantin V Korotkov
    Department of Biochemistry, University of Washington, Seattle, WA, USA
    Trends Biochem Sci 36:433-43. 2011
    ..Secretins might also play a key role in the biogenesis of their cognate secretion systems...
  85. ncbi The outer membrane protein OmpW forms an eight-stranded beta-barrel with a hydrophobic channel
    Heedeok Hong
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908, USA
    J Biol Chem 281:7568-77. 2006
    ..Taken together, the data suggest that members of the OmpW family could be involved in the transport of small hydrophobic molecules across the bacterial outer membrane...
  86. pmc The crystal structure of BamB suggests interactions with BamA and its role within the BAM complex
    Nicholas Noinaj
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 8030, USA
    J Mol Biol 407:248-60. 2011
    ..This may facilitate integration of newly synthesized OMPs into the outer membrane...
  87. pmc Evaluation of a high resolution genotyping method for Chlamydia trachomatis using routine clinical samples
    Yibing Wang
    Molecular Microbiology and Infection, School of Medicine, University of Southampton, Southampton, United Kingdom
    PLoS ONE 6:e16971. 2011
    ....
  88. pmc Structure of the cholera toxin secretion channel in its closed state
    Steve L Reichow
    Department of Biochemistry, University of Washington, Seattle, Washington, USA
    Nat Struct Mol Biol 17:1226-32. 2010
    ..By combining our results with structures of the cholera toxin and T2SS pseudopilus tip, we provide a structural basis for a possible secretion mechanism of the T2SS...
  89. pmc Crystal structure of BamD: an essential component of the β-Barrel assembly machinery of gram-negative bacteria
    Cristina M Sandoval
    Department of Chemistry and Biochemistry, University of Colorado at Boulder, USA
    J Mol Biol 409:348-57. 2011
    ....
  90. pmc Plant carbohydrate scavenging through tonB-dependent receptors: a feature shared by phytopathogenic and aquatic bacteria
    Servane Blanvillain
    Laboratoire des Interactions Plantes Microorganismes, Centre National de la Recherche Scientifique CNRS Institut National de la Recherche Agronomique INRA UMR2594 441, Castanet Tolosan, France
    PLoS ONE 2:e224. 2007
    ....
  91. pmc Ail binding to fibronectin facilitates Yersinia pestis binding to host cells and Yop delivery
    Tiffany M Tsang
    Department of Microbiology and Immunology, University of Michigan School of Medicine, 1011 N University, Ann Arbor, MI 48109 1078, USA
    Infect Immun 78:3358-68. 2010
    ..These data indicate that Ail is a key adhesin that mediates binding to host cells through interaction with fibronectin on the surface of host cells, and this interaction is important for Yop delivery by Y. pestis...
  92. ncbi Characterization of MexE-MexF-OprN, a positively regulated multidrug efflux system of Pseudomonas aeruginosa
    T Kohler
    Department of Genetics and Microbiology, Centre Medical Universitaire, Geneva, Switzerland
    Mol Microbiol 23:345-54. 1997
    ..Evidence is provided to show that the mexEF-oprN operon may be involved in the excretion of intermediates for the biosynthesis of pyocyanin, a typical secondary metabolite of P. aeruginosa...
  93. ncbi First report and detailed characterization of B. pertussis isolates not expressing Pertussis Toxin or Pertactin
    V Bouchez
    Institut Pasteur, Unité Prévention et Thérapie Moléculaires des Maladies Humaines, URA CNRS 3012, 25 rue du Dr Roux, 75015 Paris, France
    Vaccine 27:6034-41. 2009
    ..All isolates were found less pathogen in animal or cellular models; their circulation raises the problem of clinical and biological diagnoses...
  94. pmc Pertactin is required for Bordetella species to resist neutrophil-mediated clearance
    Carol S Inatsuka
    Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, Santa Barbara, California 93106 9610, USA
    Infect Immun 78:2901-9. 2010
    ....
  95. pmc Modulation of Neisseria gonorrhoeae susceptibility to vertebrate antibacterial peptides due to a member of the resistance/nodulation/division efflux pump family
    W M Shafer
    Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA 30322, USA
    Proc Natl Acad Sci U S A 95:1829-33. 1998
    ..These results indicate that such efflux systems may enable mucosal pathogens like gonococci to resist endogenous antimicrobial peptides that are thought to act during infection...
  96. ncbi Probing the ArcA-P modulon of Escherichia coli by whole genome transcriptional analysis and sequence recognition profiling
    Xueqiao Liu
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    J Biol Chem 279:12588-97. 2004
    ..A conservative but reasonable assessment is that the Arc pathway recruits 100-150 operons to mediate a role in cellular adaptation that is more extensive than hitherto anticipated...
  97. ncbi M protein, a classical bacterial virulence determinant, forms complexes with fibrinogen that induce vascular leakage
    Heiko Herwald
    Department of Cell and Molecular Biology, Lund University, Tornavagen 10, S 221 84 Lund, Sweden
    Cell 116:367-79. 2004
    ..In addition, M protein/fibrinogen complexes were identified in tissue biopsies from a patient with necrotizing fasciitis and STSS, further underlining the pathogenic significance of such complexes in severe streptococcal infections...
  98. pmc Antigenic and genetic heterogeneity of Borrelia burgdorferi populations transmitted by ticks
    J Ohnishi
    Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, NC 27599, USA
    Proc Natl Acad Sci U S A 98:670-5. 2001
    ..burgdorferi transmission. Implicit in our model is the concept that tick transmission converts a homogeneous spirochete population into a heterogeneous population that is poised to infect the mammalian host...
  99. ncbi Properties and clinical performance of vaccines containing outer membrane vesicles from Neisseria meningitidis
    Johan Holst
    Department of Bacteriology and Immunology, Norwegian Institute of Public Health, Oslo, P O Box 4404 Nydalen, Oslo NO 0403, Norway
    Vaccine 27:B3-12. 2009
    ..The desire to have a global vaccine strategy that enables susceptible individuals to be protected against all the relevant serogroups of meningococcal disease may become a reality...
  100. ncbi Crystal structures of various maltooligosaccharides bound to maltoporin reveal a specific sugar translocation pathway
    R Dutzler
    Department of Structural Biology, Biozentrum, University of Basel, CH 4056 Basel, Switzerland
    Structure 4:127-34. 1996
    ..coli. In particular, it is indispensable for the transport of long maltooligosaccharides, as these do not pass through non-specific porins. An understanding of this intriguing capability requires elucidation of the structural basis...
  101. ncbi Overexpression of the mexC-mexD-oprJ efflux operon in nfxB-type multidrug-resistant strains of Pseudomonas aeruginosa
    K Poole
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    Mol Microbiol 21:713-24. 1996
    ..These data indicate that the multidrug resistance of nfxB strains is due to overexpression of an efflux operon, mexC-mexD-oprJ, encoding components of a second efflux pump in P. aeruginosa...

Research Grants72

  1. Structure and dynamics of bacterial membrane protein - receptor interactions
    LINDA M COLUMBUS; Fiscal Year: 2013
    ..The goal of this research is to investigate the structure and dynamics of bacterial outer membrane proteins and their interactions with host receptors...
  2. Engineered Nanopores for Single-Molecule Stochastic Sensing
    Liviu Movileanu; Fiscal Year: 2013
    ..We will use Ferric hydroxamate uptake component A (FhuA), one of the members of the superfamily of bacterial outer membrane proteins. Molecular engineering of the FhuA protein will be used in single-molecule stochastic sensing, ..
  3. EFFECT OF PORA ON MENINGOCOCCI-MACROPHAGE INTERACTION
    Lillian Waldbeser; Fiscal Year: 1999
    ..Dr. Waldbeser proposes to study the meningococcal-macrophage interaction. Bacterial outer membrane proteins play a part in this interaction...
  4. Genetic Determinants of Anaplasma Transmission
    SUSAN NOH; Fiscal Year: 2004
    ..The sponsor has an active research program in vector borne disease has successfully mentored clinicians, graduate students, and post-doctoral fellows to research independence. ..
  5. Antigenic variation in rickettsial transmission
    Guy Palmer; Fiscal Year: 2008
    ..This hypothesis will be addressed using comprehensive identification of the variant population in the reservoir host and tick vector and testing whether immune responses against a broad array of variants prevents superinfection. ..
  6. Immune Responses in Genital Chlamydia Prior to Therapy
    WILLIAM GEISLER; Fiscal Year: 2005
    ..abstract_text> ..
  7. DEVELOPMENT OF A VACCINE FOR MORAXELLA CATARRHALIS
    Timothy Murphy; Fiscal Year: 2009
    ..catarrhalis surface, the identification of surface antigens expressed specifically during human infection and critical information about the human immune response to surface antigens of M. catarrhalis. ..
  8. Forensic Detection of Salmonella Resistance and Subtype
    Lee Harrison; Fiscal Year: 2005
    ..enterica and 2) molecular methods for rapid outbreak detection among S. enterica serovars. ..
  9. Siderophore transport mechanism via membrane receptors
    Ranjan Chakraborty; Fiscal Year: 2004
    ..For the mutant proteins showing normal binding and defective transport, methods of purification and crystallization will be standardized. ..
  10. Structure-Function of Borrelia Surface Lipoproteins
    WOLFRAM ZUECKERT; Fiscal Year: 2005
    ..Furthermore, they will yield important clues for the design of future intervention strategies. ..
  11. LYME ARTHRITIS: A NEW EPIDEMIC DISEASE
    Allen C Steere; Fiscal Year: 2010
    ..Furthermore, this work brings us closer to the long-term goal of direct comparisons of antigens in Lyme arthritis and rheumatoid arthritis synovia. ..
  12. Analysis of Borrelia Burgdorferi Erp Proteins
    Brian Stevenson; Fiscal Year: 2010
    ..Results of our studies will provide important insight into the mechanisms by which B. burgdorferi infects humans. ..
  13. Diagnosis and Pathogenesis of Early Lyme Disease
    Allen Steere; Fiscal Year: 2007
    ..In addition, greater knowledge of spirochetal virulence and host factors associated with severe disease may allow the development of laboratory markers for patients who would benefit from more intensive treatment. ..
  14. Lipoprotein Transport in Borrelia Spirochetes
    WOLFRAM R ZUECKERT; Fiscal Year: 2010
    ....
  15. MOLECULAR PROPERTIES AND MODULATION OF BACTERIAL PORINS
    ANNE DELCOUR; Fiscal Year: 2003
    ..This information on the three-dimensional structure of porins will be extremely valuable for the design of genetically engineered channels and the meaningful interpretation of the data. ..
  16. Borrelia burgdorferi LuxS-mediated quorum sensing
    Brian Stevenson; Fiscal Year: 2007
    ..burgdorferi and its hosts, as well as identifying potential bacterial targets for improved therapies to prevent and treat Lyme disease. ..
  17. Export & Import of Lethal Agents Mediated by TolC
    Rajeev Misra; Fiscal Year: 2006
    ..Besides the obvious role of TolC in antibiotic resistance, its ability to mediate the secretion of a variety of virulence factors in pathogenic bacteria makes it a medically important subject of investigation. ..
  18. Recurrent otitis media and COPD: immunity and vaccines
    Timothy Murphy; Fiscal Year: 2004
    ..The proposed studies will advance the field of vaccine development to prevent otitis media and respiratory tract infections in COPD. ..
  19. Human Lung Chromium Toxicity: Role of cytochrome b5
    Charles Myers; Fiscal Year: 2007
    ..They will provide a deeper understanding of the mechanisms underlying Cr(VI) toxicity in human lung, and will lead to new insights on the role of b5 in toxicant activation [unreadable] [unreadable]..
  20. 2008 Bacterial Cell Surfaces Gordon Research Conference
    ANNE DELCOUR; Fiscal Year: 2008
    ..Success in these research endeavors is enhanced by the exchange of information and ideas promoted by the Bacterial Cell Surfaces Gordon Conference. [unreadable] [unreadable] [unreadable]..
  21. Resistant E.coli in Humans and Poultry
    James Johnson; Fiscal Year: 2005
    ..Does the research address a current and compelling problem of antimicrobial resistance that is of high public health importance and for which research is needed? [yes]. ..
  22. BACTERIAL SURFACE PROTEINS: POTENTIAL TARGETS FOR SEPSIS
    Judith Hellman; Fiscal Year: 2004
    ..The ultimate goal of the candidate is to apply the fundamental knowledge of mechanisms involved in sepsis to develop anti-sepsis strategies that target bacterial toxins. ..
  23. Plant Biofilm Inhibitors to Discover Biofilm Genes
    Thomas Wood; Fiscal Year: 2008
    ..deduce a model for bacterial biofilm formation based on the genetic information (which genes induced/repressed) and protein information discerned (via NMR) ..
  24. Hunter Killer Peptides: Membrane Interactions and Receptor Binding
    Leigh Plesniak; Fiscal Year: 2007
    ..A second HKP that kills white fat tissue reduces fatty deposits and has a positive impact on glucose metabolism, reducing symptoms of diabetes. [unreadable] [unreadable] [unreadable]..
  25. C. trachomatis genomics, strain typing, and evolution
    Deborah Dean; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  26. Extracellular Proteins in Virulence of B. (pseudo)mallei
    Mark Schell; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  27. Engineering Large scale pathways from organisms to Escherichia coli
    James Liao; Fiscal Year: 2008
    ..In particular, selected projects proposed here will be used as modules in a workshop established by FRT at Ohio State and form the basis for a course (Metabolic Engineering) at UCLA. ..
  28. Desferrithiocin Analogue Actinide Decorporation Agents
    RAYMOND BERGERON; Fiscal Year: 2008
    ..abstract_text> ..
  29. Novel Mechanism of Uranium Reduction Via Microbial Nanowires
    Gemma Reguera; Fiscal Year: 2011
    ....
  30. Complexes of CAP in Transcription Activation
    Catherine Lawson; Fiscal Year: 2009
    ..The proposed studies of the CAP system provide a means to rapidly advance our understanding of fundamental structural principles underlying transcriptional control. ..
  31. Cellular Immunity to Chlamydua at the Epithelial Interface
    RAYMOND MORRIS JOHNSON; Fiscal Year: 2010
    ..Defining what protective T cells look like, and how they function, will be critical for designing and assessing future Chlamydia candidate vaccines. ..
  32. Regulation of Recall Responses During Ehrlichiosis
    Gary M Winslow; Fiscal Year: 2010
    ..By defining optimal CD4 T cell immune correlates, and the role of memory CD8 T cells during recall responses, the proposed studies will contribute to the development of therapies and vaccines for ehrlichioses and related infections. ..
  33. 2004 Microbial Stress Response Gordon Conference
    Carol Gross; Fiscal Year: 2004
    ..This meeting is generally oversubscribed and given the current program is likely to have very wide appeal. ..
  34. Chemogenomic Analysis of E. coli Response to NO species
    James C Liao; Fiscal Year: 2010
    ..If hew TF targets are predicted, then the iteration continues. Otherwise, the predicted networks are verified using genetic and biochemical experiments. ..
  35. Mechanism of Host Cell Invasion by Microbial Pathogens
    Partho Ghosh; Fiscal Year: 2010
    ..In addition, the particular pathogen we are studying, Listeria monocytogenes, affects a protein called Met that is involved in some cancers. Thus, results from our studies may also have an impact on cancer therapies. ..
  36. Structural Biology of Cancer Related Membrane Proteins Expressed in P. Pastoris
    Michael Wiener; Fiscal Year: 2009
    ..We will use an automated plate imagerto view and document crystallization experiments. We will solve the structures of CRIMPs that form high quality crystals. ..
  37. Improved Methods for Membrane Protein Crystallization
    Michael Wiener; Fiscal Year: 2009
    ..Other planned targets will focus upon other human membrane proteins of fundamental and biomedical import. ..
  38. Small-Molecule Inhibitors of Vaccinia-H1-Related Phosphatase VHR
    Lutz Tautz; Fiscal Year: 2008
    ..PTPs have been recently implicated with numerous human diseases, including cancer. Recent studies by our lab and others clearly suggest that VHR is critical for the development of cervical cancer. [unreadable] [unreadable] [unreadable]..
  39. Bioorganic mechanism of peptide antibiotics
    Dewey McCafferty; Fiscal Year: 2009
    ..In turn, we hope to apply the knowledge gained to the development of alternative antibiotics with improved activity against infections due to resistant and virulent bacterial phenotypes. ..
  40. Srv is critical for Streptococcal survival in the host
    SEAN REID; Fiscal Year: 2009
    ..Understanding the mechanism of Srv-dependent gene regulation will provide new insights into the control of GAS disease. ..
  41. Topical Antibiotics to Prevent Lyme Disease
    Gary Wormser; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  42. Regulation of Cellular division in M. tuberculosis
    RICHARD SLAYDEN; Fiscal Year: 2006
    ..Thus, the studies proposed in this application are designed to examine the replication dynamics of MTB, specifically focusing on cell cycle-regulated genes that are involved in cell division. [unreadable] [unreadable] [unreadable]..
  43. American Society for Rickettsiology conference
    ROMAN GANTA; Fiscal Year: 2005
    ..abstract_text> ..
  44. HELICOBACTER DIAGNOSTIC ASSAYS FOR LABORATORY MICE
    Stephen Barthold; Fiscal Year: 2004
    ..abstract_text> ..
  45. Prevotella intermedia: Iron and Virulence
    JANINA LEWIS; Fiscal Year: 2004
    ..intermedia. Since P. intermedia is resistant to many antibiotics alternative methods of preventive measures are needed for this bacterium. ..
  46. ANTIGEN DELIVERY SYSTEM DESIGN FOR HOST DEFENSE ANALYSIS
    Roy Curtiss; Fiscal Year: 2004
    ..As improved antigen delivery systems are discovered, it is anticipated that they will construct S. typhi or S. paratyphi A derivatives thereof for eventual clinical studies in humans. ..
  47. Nuclease-Resistant Aptamers to Botox and Anthrax Toxins
    John Bruno; Fiscal Year: 2004
    ..Such reagents could also be used in environmental biosensors or diagnostic test kits for detection of BOTOX and anthrax toxins in body fluids or environmental samples. ..
  48. 3RD ANNUAL MUTANT MOUSE REGIONAL RESOURCE CENTER MEETING
    Stephen Barthold; Fiscal Year: 2003
    ....
  49. MYCOBACTERIAL CELL WALL RECOGNITION BY MACROPHAGES
    Kelly Smith; Fiscal Year: 2002
    ..tuberculosis, and disclose fundamental principles of innate immunity in infectious and inflammatory diseases. ..
  50. ROLES OF EHRLICHIAL OUTER MEMBRANE PROTEIN IN PARASITISM
    Yasuko Rikihisa; Fiscal Year: 2001
    ..Aim 5 addresses hypothesis 3 by examining the effects of immunization with the ehrlichial OMP in a mouse model of human ehrlichiosis. ..
  51. PERIPHERAL BENZODIAZEPINE RECEPTOR STRUCTURAL BIOLOGY
    Michael Wiener; Fiscal Year: 2006
    ....
  52. HIV-1 ASSOCIATED SUPERANTIGEN
    DAVID POSNETT; Fiscal Year: 2001
    ..Another aim is to investigate whether the SAG enhances CMV infection or reactivation. Finally a potential role of the CMV SAG in enhancing HIV infection will be explored. ..
  53. American Society for Rickettsiology Conference 2006
    ROMAN GANTA; Fiscal Year: 2006
    ..abstract_text> ..
  54. Disruption of Innate Immune Function by P.Gingivalis Phosphotidylethanolamine Lip
    Toshihisa Kawai; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  55. Pathogenesis of Persistent Chlamydial STDs
    Deborah Dean; Fiscal Year: 2007
    ..1 Description, ..
  56. Structures of Bacterial Membrane-Bound Oxidoreductases
    Joel Weiner; Fiscal Year: 2006
    ....
  57. MOLECULAR GENETIC ANALYSIS OF SALMONELLA PATHOGENICITY
    Roy Curtiss; Fiscal Year: 2006
    ..All experiments will be conducted under conditions that preclude infections of workers and inadvertent release of infectious microorganisms. ..
  58. T.pallidum Membrane Immunogens and Anti-Oxidant Defense
    Justin Radolf; Fiscal Year: 2006
    ..pallidum (Specific Aim Two); and characterize key components of T. pallidum's defense against oxidative stress (Specific Aim Three). ..
  59. Non-Invasive Approaches to Assessing Inflammation (RMI)
    RYAN SARTOR; Fiscal Year: 2006
    ..This project will create a new program involving investigators who have not previously worked together, while building on institutional strengths and programmatic initiatives. ..
  60. GENETIC CONTROL OF NITRATE RESPIRATION IN E. COLI
    Valley J Stewart; Fiscal Year: 2010
    ..Thus, results from the model species E. coli enhance understanding for a broad range of pathogens that significantly impact public health. ..
  61. Porins of Mycobacterium tuberculosis
    Michael Niederweis; Fiscal Year: 2009
    ..These studies will not only be essential for the design of new TB drugs, but will also be of fundamental interest for our understanding of nutrient uptake by M. tuberculosis. ..
  62. Mouse model for sepsis
    H Warren; Fiscal Year: 2009
    ..abstract_text> ..
  63. 5th Comparative Medicine Resource Center Director Mtg.
    Stephen Barthold; Fiscal Year: 2008
    ....
  64. MEMBRANE PROTEIN STRUCTURE : USE OF LIPIDIC MESOPHASES
    Martin Caffrey; Fiscal Year: 2004
    ..The results of these studies, together with an analysis in detail of the crystal/lipid interface will then be used in attempts at rational crystallization of the previously uncrystallized photosystem II from Chlamydomonas. ..
  65. Role of Yersinia Yops in an Animal Infection Model
    Joan Mecsas; Fiscal Year: 2007
    ..to colonize and cause disease in animal tissues and to understand the host defenses that prevent, combat and/or contain Yersinia infection in tissues. ..
  66. Inhibitors of Yersinia Yop Type III Secretion(RMI)
    Joan Mecsas; Fiscal Year: 2005
    ..In addition, these inhibitors will be valuable tools to probe the structure and function of type III secretion systems encoded by Yersinia species and other Gram-negative bacterial pathogens. ..
  67. INTERMEDIATES AND TRANSBILAYER PEPTIDES
    Martin Caffrey; Fiscal Year: 2001
    ..Such activity is consistent with Dr. Caffrey's hypothesis. ..
  68. IRON CHELATORS PREDICATED ON DESFERRITHIOCIN
    RAYMOND BERGERON; Fiscal Year: 2004
    ....
  69. Membrane Proteins, Crystallogenesis and X-ray Structure
    Martin Caffrey; Fiscal Year: 2009
    ..Success in obtaining crystals, and ultimately the atomic structure of membrane proteins, will enhance our understanding of some of the most fundamental processes underlying cellular function that are integral to human health. ..
  70. Genetics of Survival of Yersinia pseudotuberculosis
    Joan Mecsas; Fiscal Year: 2002
    ..In addition, studies of yop mutant Yersinia strains in mouse strains with specific immune defects may indicate the facets of the immune system targeted by each Yop. ..