xenopus proteins

Summary

Summary: Proteins obtained from various species of Xenopus. Included here are proteins from the African clawed frog (XENOPUS LAEVIS). Many of these proteins have been the subject of scientific investigations in the area of MORPHOGENESIS and development.

Top Publications

  1. pmc The genome of the Western clawed frog Xenopus tropicalis
    Uffe Hellsten
    Department of Energy Joint Genome Institute, Walnut Creek, CA 94598, USA
    Science 328:633-6. 2010
  2. pmc Functional uncoupling of MCM helicase and DNA polymerase activities activates the ATR-dependent checkpoint
    Tony S Byun
    Department of Molecular Pharmacology, Stanford University, Stanford, CA 94305, USA
    Genes Dev 19:1040-52. 2005
  3. pmc Dorsal-ventral patterning and neural induction in Xenopus embryos
    Edward M De Robertis
    Howard Hughes Medical Institute, Department of Biological Chemistry, University of California Los Angeles, Los Angeles, CA 90095 1662, USA
    Annu Rev Cell Dev Biol 20:285-308. 2004
  4. ncbi Maternal wnt11 activates the canonical wnt signaling pathway required for axis formation in Xenopus embryos
    Qinghua Tao
    Division of Developmental Biology, Cincinnati Children s Research Foundation, 3333 Burnet Avenue, Cincinnati, Ohio 45229, USA
    Cell 120:857-71. 2005
  5. ncbi Biochemical and cellular effects of roscovitine, a potent and selective inhibitor of the cyclin-dependent kinases cdc2, cdk2 and cdk5
    L Meijer
    CNRS, Station Biologique, Roscoff, France
    Eur J Biochem 243:527-36. 1997
  6. ncbi TopBP1 activates the ATR-ATRIP complex
    Akiko Kumagai
    Division of Biology 216 76, California Institute of Technology, Pasadena, CA 91125, USA
    Cell 124:943-55. 2006
  7. ncbi Control of beta-catenin phosphorylation/degradation by a dual-kinase mechanism
    Chunming Liu
    Division of Neuroscience, Children s Hospital, Department of Neurology, Harvard Medical School, Boston, MA 02115, USA
    Cell 108:837-47. 2002
  8. pmc Mcm10 and And-1/CTF4 recruit DNA polymerase alpha to chromatin for initiation of DNA replication
    Wenge Zhu
    Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Genes Dev 21:2288-99. 2007
  9. pmc Collective chemotaxis requires contact-dependent cell polarity
    Eric Theveneau
    Department of Cell and Developmental Biology, University College London, London WC1E 6BT, UK
    Dev Cell 19:39-53. 2010
  10. pmc Regulated activity of PP2A-B55 delta is crucial for controlling entry into and exit from mitosis in Xenopus egg extracts
    Satoru Mochida
    Cancer Research UK, London Research Institute, Clare Hall Laboratories, South Mimms, Herts, UK
    EMBO J 28:2777-85. 2009

Research Grants

  1. MARCKS Phosphorylation in Growth Cone Signaling
    JESSE GATLIN; Fiscal Year: 2004
  2. Computation tools for analyzing gene expression data
    Naama Barkai; Fiscal Year: 2004
  3. THE ROLE OF MAP KINASE IN THE CELL CYCLE
    JAMES FERRELL; Fiscal Year: 2005
  4. Intrinsic Spindle Mechanisms to Insure Bipolarity
    JESSE GATLIN; Fiscal Year: 2007
  5. MOLECULAR GENETICS OF EARLY NEUROGENESIS
    Ethan Bier; Fiscal Year: 2007
  6. BIOLOGICAL FUNCTIONS OF TOPOISOMERASE I IN S CEREVISIAE
    Michael Christman; Fiscal Year: 2000
  7. BIOLOGICAL FUNCTIONS OF TOPOISOMERASE I IN S CEREVISIAE
    Michael Christman; Fiscal Year: 1999
  8. METHYLATION OF ATYPICAL PROTEIN ASPARTYL RESIDUES
    CLARE O CONNOR; Fiscal Year: 1990
  9. THE ROLE OF MAP KINASE IN THE CELL CYCLE
    JAMES FERRELL; Fiscal Year: 2002
  10. THE ROLE OF MAP KINASE IN THE CELL CYCLE
    JAMES FERRELL; Fiscal Year: 2002

Detail Information

Publications253 found, 100 shown here

  1. pmc The genome of the Western clawed frog Xenopus tropicalis
    Uffe Hellsten
    Department of Energy Joint Genome Institute, Walnut Creek, CA 94598, USA
    Science 328:633-6. 2010
    ..The genome exhibits substantial shared synteny with human and chicken over major parts of large chromosomes, broken by lineage-specific chromosome fusions and fissions, mainly in the mammalian lineage...
  2. pmc Functional uncoupling of MCM helicase and DNA polymerase activities activates the ATR-dependent checkpoint
    Tony S Byun
    Department of Molecular Pharmacology, Stanford University, Stanford, CA 94305, USA
    Genes Dev 19:1040-52. 2005
    ....
  3. pmc Dorsal-ventral patterning and neural induction in Xenopus embryos
    Edward M De Robertis
    Howard Hughes Medical Institute, Department of Biological Chemistry, University of California Los Angeles, Los Angeles, CA 90095 1662, USA
    Annu Rev Cell Dev Biol 20:285-308. 2004
    ..Many of the molecular players that regulate D-V patterning via regulation of BMP signaling have been conserved between Drosophila and the vertebrates...
  4. ncbi Maternal wnt11 activates the canonical wnt signaling pathway required for axis formation in Xenopus embryos
    Qinghua Tao
    Division of Developmental Biology, Cincinnati Children s Research Foundation, 3333 Burnet Avenue, Cincinnati, Ohio 45229, USA
    Cell 120:857-71. 2005
    ..Second, we show that activation requires the glycosyl transferase X.EXT1. Third, we find that the EGF-CFC protein, FRL1, is also essential and interacts with Wnt11 to activate canonical Wnt signaling...
  5. ncbi Biochemical and cellular effects of roscovitine, a potent and selective inhibitor of the cyclin-dependent kinases cdc2, cdk2 and cdk5
    L Meijer
    CNRS, Station Biologique, Roscoff, France
    Eur J Biochem 243:527-36. 1997
    ..Through its unique selectivity for some cyclin-dependent kinases, roscovitine provides a useful antimitotic reagent for cell cycle studies and may prove interesting to control cells with deregulated cdc2, cdk2 or cdk5 kinase activities...
  6. ncbi TopBP1 activates the ATR-ATRIP complex
    Akiko Kumagai
    Division of Biology 216 76, California Institute of Technology, Pasadena, CA 91125, USA
    Cell 124:943-55. 2006
    ..These studies establish that activation of ATR by TopBP1 is a crucial step in the initiation of ATR-dependent signaling processes...
  7. ncbi Control of beta-catenin phosphorylation/degradation by a dual-kinase mechanism
    Chunming Liu
    Division of Neuroscience, Children s Hospital, Department of Neurology, Harvard Medical School, Boston, MA 02115, USA
    Cell 108:837-47. 2002
    ..Our study uncovers distinct roles and steps of beta-catenin phosphorylation, identifies CKIalpha as a component in Wnt/beta-catenin signaling, and has implications to pathogenesis/therapeutics of human cancers and diabetes...
  8. pmc Mcm10 and And-1/CTF4 recruit DNA polymerase alpha to chromatin for initiation of DNA replication
    Wenge Zhu
    Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Genes Dev 21:2288-99. 2007
    ..The discovery also adds to the connection between replication initiation and sister chromatid cohesion...
  9. pmc Collective chemotaxis requires contact-dependent cell polarity
    Eric Theveneau
    Department of Cell and Developmental Biology, University College London, London WC1E 6BT, UK
    Dev Cell 19:39-53. 2010
    ....
  10. pmc Regulated activity of PP2A-B55 delta is crucial for controlling entry into and exit from mitosis in Xenopus egg extracts
    Satoru Mochida
    Cancer Research UK, London Research Institute, Clare Hall Laboratories, South Mimms, Herts, UK
    EMBO J 28:2777-85. 2009
    ..Increasing the concentration of PP2A-B55 delta in extracts by adding recombinant enzyme inhibited the entry into mitosis. This form of PP2A seems to be a key regulator of entry into and exit from mitosis...
  11. pmc MCM2-7 form double hexamers at licensed origins in Xenopus egg extract
    Agnieszka Gambus
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, United Kingdom
    J Biol Chem 286:11855-64. 2011
    ..The CMG proteins also associate less strongly with other replication proteins, consistent with the idea that a single CMG complex forms the core of the replisome...
  12. doi Hairy2 functions through both DNA-binding and non DNA-binding mechanisms at the neural plate border in Xenopus
    Massimo Nichane
    Laboratoire d Embryologie Moleculaire, Universite Libre de Bruxelles, Institut de Biologie et de Médecine Moléculaires IBMM, Gosselies, Belgium
    Dev Biol 322:368-80. 2008
    ..Together, these results suggest that Hairy2 has a dual mode of action and may function at the neural plate border through both a DNA-binding and a non-DNA-binding Stat3-Delta1 mediated mechanism...
  13. ncbi Initiation of DNA replication requires the RECQL4 protein mutated in Rothmund-Thomson syndrome
    Mahesh N Sangrithi
    Cancer Research UK Department of Oncology, University of Cambridge, Hutchison MRC Research Centre, Hills Road, Cambridge CB2 2XZ, United Kingdom
    Cell 121:887-98. 2005
    ..This role connects defective replication initiation to a chromosome-fragility disorder...
  14. pmc The miR-30 miRNA family regulates Xenopus pronephros development and targets the transcription factor Xlim1/Lhx1
    Raman Agrawal
    Department of Cell Biology and Anatomy, LSU Health Sciences Center, MEB 6A12, 1901 Perdido Street, New Orleans, LA 70112, USA
    Development 136:3927-36. 2009
    ..However, in the absence of miR-30 activity, Xlim1/Lhx1 is maintained at high levels and, therefore, may contribute to the delayed terminal differentiation of the amphibian pronephros...
  15. ncbi XTcf-3 transcription factor mediates beta-catenin-induced axis formation in Xenopus embryos
    M Molenaar
    Department of Immunology University Hospital, Utrecht The Netherlands
    Cell 86:391-9. 1996
    ..We propose that signaling by beta-catenin involves complex formation with XTcf-3, followed by nuclear translocation and activation of specific XTcf-3 target genes...
  16. pmc The structural determinants of checkpoint activation
    Christina A MacDougall
    Department of Chemical and Systems Biology, Stanford University, Stanford, California 94305, USA
    Genes Dev 21:898-903. 2007
    ..These observations define the minimal DNA requirements for checkpoint activation and suggest that primed ssDNA represents a common checkpoint activating-structure formed following many types of damage...
  17. pmc Neural induction in the absence of mesoderm: beta-catenin-dependent expression of secreted BMP antagonists at the blastula stage in Xenopus
    O Wessely
    Howard Hughes Medical Institute, University of California, Los Angeles, California 90095-1662, USA
    Dev Biol 234:161-73. 2001
    ..The present results suggest an additional function for beta-Catenin, the early activation of expression of secreted BMP antagonists, such as Chordin, in a preorganizer region in the dorsal side of the Xenopus blastula...
  18. ncbi Vertebrate hairy and Enhancer of split related proteins: transcriptional repressors regulating cellular differentiation and embryonic patterning
    R L Davis
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Oncogene 20:8342-57. 2001
    ....
  19. ncbi Direct binding of the PDZ domain of Dishevelled to a conserved internal sequence in the C-terminal region of Frizzled
    Hing C Wong
    Department of Structural Biology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Mol Cell 12:1251-60. 2003
    ..Together, our results identify a missing molecular connection within the Wnt pathway. Differences in the binding affinity of the Dvl PDZ domain and its binding partners may be important in regulating signal transduction by Dvl...
  20. ncbi Synergism between Pax-8 and lim-1 in embryonic kidney development
    T J Carroll
    Cell and Developmental Biology, University of Texas, Austin, Texas, 78712, USA
    Dev Biol 214:46-59. 1999
    ..Together these data indicate that the interaction between XPax-8 and Xlim-1 is a key early step in the establishment of the pronephric primordium...
  21. ncbi XCDT1 is required for the assembly of pre-replicative complexes in Xenopus laevis
    D Maiorano
    Institute of Human Genetics, CNRS, Genome Dynamics and Development, Montpellier, France
    Nature 404:622-5. 2000
    ..These findings indicate that XCDT1 is an essential component of the system that regulates origins firing during S phase...
  22. pmc GEMC1 is a TopBP1-interacting protein required for chromosomal DNA replication
    Alessia Balestrini
    Genome Stability, London Research Institute, Hertfordshire, UK
    Nat Cell Biol 12:484-91. 2010
    ..These data suggest that GEMC1 promotes initiation of chromosomal DNA replication in multicellular organisms by mediating TopBP1- and Cdk2-dependent recruitment of Cdc45 onto replication origins...
  23. ncbi Cerberus is a head-inducing secreted factor expressed in the anterior endoderm of Spemann's organizer
    T Bouwmeester
    Howard Hughes Medical Institute and Department of Biological Chemistry, University of California, Los Angeles, 90095 1737, USA
    Nature 382:595-601. 1996
    ..The results suggest a role for a molecule expressed in the anterior endoderm in the induction of head structures in the vertebrate embryo...
  24. ncbi Smad4 and FAST-1 in the assembly of activin-responsive factor
    X Chen
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115 5730, USA
    Nature 389:85-9. 1997
    ..These results indicate that Smad4 stabilizes a ligand-stimulated Smad2-FAST-1 complex as an active DNA-binding factor...
  25. pmc The N-terminal noncatalytic region of Xenopus RecQ4 is required for chromatin binding of DNA polymerase alpha in the initiation of DNA replication
    Kumiko Matsuno
    Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560 0043, Japan
    Mol Cell Biol 26:4843-52. 2006
    ..Our data suggest that RecQ4 performs an essential role in the assembly of replication machinery through interaction with Cut5 in vertebrates...
  26. ncbi Expression of Pax-3 is initiated in the early neural plate by posteriorizing signals produced by the organizer and by posterior non-axial mesoderm
    A G Bang
    Molecular Neurobiology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Development 124:2075-85. 1997
    ....
  27. pmc Repression of origin assembly in metaphase depends on inhibition of RLF-B/Cdt1 by geminin
    S Tada
    CRC Chromosome Replication Research Group, Wellcome Trust Biocentre, Dow Street University of Dundee, Dundee DD1 5EH, UK
    Nat Cell Biol 3:107-13. 2001
    ..These experiments suggest that geminin-mediated inhibition of RLF-B/Cdt1 is essential for repressing origin assembly late in the cell cycle of higher eukaryotes...
  28. pmc CENP-E combines a slow, processive motor and a flexible coiled coil to produce an essential motile kinetochore tether
    Yumi Kim
    Ludwig Institute for Cancer Research, University of California, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 181:411-9. 2008
    ..We propose that the highly processive microtubule-dependent motor activity of CENP-E serves to power chromosome congression and provides a flexible, motile tether linking kinetochores to dynamic spindle microtubules...
  29. ncbi Xenopus hairy2 functions in neural crest formation by maintaining cells in a mitotic and undifferentiated state
    Kan ichiro Nagatomo
    Department of Biology, Graduate School of Science, Osaka University, and JT Biohistory Research Hall, Osaka, Japan
    Dev Dyn 236:1475-83. 2007
    ..Furthermore, the depletion of Xhairy2 leads to the apoptosis of mitotic cells. Our results suggest that Xhairy2 functions in neural crest specification by maintaining cells in the mitotic and undifferentiated state...
  30. pmc Oct25 represses transcription of nodal/activin target genes by interaction with signal transducers during Xenopus gastrulation
    Ying Cao
    Institute of Biochemistry, University of Ulm, Ulm, Germany
    J Biol Chem 283:34168-77. 2008
    ..Our results provide a novel view in that Oct25 controls the nodal/activin pathway and thus maintains the undifferentiated state of embryonic cells in preventing them from premature differentiation...
  31. ncbi Zebrafish Rho kinase 2 acts downstream of Wnt11 to mediate cell polarity and effective convergence and extension movements
    Florence Marlow
    Department of Biological Sciences, Vanderbilt University, VU Station B 351634, Nashville, TN 37235 1634, USA
    Curr Biol 12:876-84. 2002
    ..Moreover, how defective mediolateral cell polarity impacts CE is not understood...
  32. doi ATP4a is required for Wnt-dependent Foxj1 expression and leftward flow in Xenopus left-right development
    Peter Walentek
    University of Hohenheim, Institute of Zoology, Garbenstrasse 30, 70593 Stuttgart, Germany
    Cell Rep 1:516-27. 2012
    ..Specifically, ATP4 was required for Wnt/β-catenin-regulated Foxj1 induction and Wnt/PCP-dependent cilia polarization. Our work argues for evolutionary conservation of symmetry breakage in the vertebrates...
  33. pmc POU-V factors antagonize maternal VegT activity and beta-Catenin signaling in Xenopus embryos
    Ying Cao
    Institute of Biochemistry, University of Ulm, Albert Einstein Allee 11, 89081 Ulm, Germany
    EMBO J 26:2942-54. 2007
    ..We suggest that POU-V factors antagonize primary inducers to allow germ layer specification in a temporally and spatially coordinated manner...
  34. ncbi Frizzled7 mediates canonical Wnt signaling in neural crest induction
    Muhammad Abu-Elmagd
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
    Dev Biol 298:285-98. 2006
    ..We therefore demonstrate that Xfz7 is regulated by BMP antagonism and is required for neural crest induction by Wnt in the developing vertebrate embryo...
  35. ncbi The Notch-effector HRT1 gene plays a role in glomerular development and patterning of the Xenopus pronephros anlagen
    Vincent Taelman
    Laboratoire d Embryologie Moleculaire, Universite Libre de Bruxelles, Institut de Biologie et de Médecine Moléculaires IBMM, Gosselies, Belgium
    Development 133:2961-71. 2006
    ..Together, our results provide evidence that XHRT1 plays an important role in glomerular development and early proximodistal patterning that is distinct from those of the other pronephric bHLH repressors...
  36. pmc Dynamic interactions of high Cdt1 and geminin levels regulate S phase in early Xenopus embryos
    Jolanta Kisielewska
    University of Newcastle, The Institute for Cell and Molecular Biosciences, Framlington Place, NE2 4HH, Newcastle upon Tyne, UK
    Development 139:63-74. 2012
    ....
  37. ncbi A heterodimeric coiled-coil protein required for mitotic chromosome condensation in vitro
    T Hirano
    Department of Pharmacology, University of California, San Francisco 94143 0450
    Cell 79:449-58. 1994
    ..These motifs are highly conserved in a protein family, members of which have been identified recently in both prokaryotes and eukaryotes...
  38. pmc Traf2- and Nck-interacting kinase is essential for canonical Wnt signaling in Xenopus axis formation
    Reiko Satow
    Chemotherapy Division, National Cancer Center Research Institute, Chuo Ku, Tokyo 104 0045, Japan
    J Biol Chem 285:26289-94. 2010
    ..These results demonstrate that XTNIK is an essential factor for the transcriptional activity of the beta-catenin-TCF complex and dorsal axis determination in Xenopus embryos...
  39. ncbi Xenopus Staufen is a component of a ribonucleoprotein complex containing Vg1 RNA and kinesin
    Young J Yoon
    Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI 02912, USA
    Development 131:3035-45. 2004
    ..Our results suggest a central role for XStau in RNA localization in Xenopus oocytes, and provide evidence that Staufen is a conserved link between specific mRNAs and the RNA localization machinery...
  40. ncbi Haploinsufficient phenotypes in Bmp4 heterozygous null mice and modification by mutations in Gli3 and Alx4
    N R Dunn
    Department of Cell Biology, Vanderbilt University Medical School, Nashville, Tennessee, 37232 2175, USA
    Dev Biol 188:235-47. 1997
    ..These genetic interactions suggest a model for the multigenic control of anterior digit patterning during vertebrate limb development...
  41. pmc From expression cloning to gene modeling: the development of Xenopus gene sequence resources
    Michael J Gilchrist
    Division of Systems Biology, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London, United Kingdom
    Genesis 50:143-54. 2012
    ..In addition, I demonstrate that the gaps in the current genome assembly create problems for the computational elucidation of gene sequences, and suggest some ways to ameliorate the effects of this...
  42. pmc Early, nonciliary role for microtubule proteins in left-right patterning is conserved across kingdoms
    Maria Lobikin
    Department of Biology, Center for Regenerative and Developmental Biology, Tufts University, Medford, MA 02155, USA
    Proc Natl Acad Sci U S A 109:12586-91. 2012
    ..The origin of laterality is cytoplasmic, ancient, and highly conserved across kingdoms, a fundamental feature of the cytoskeleton that underlies chirality in cells and multicellular organisms...
  43. ncbi The status of Wnt signalling regulates neural and epidermal fates in the chick embryo
    S I Wilson
    Department of Microbiology, , , Sweden
    Nature 411:325-30. 2001
    ..Conversely, a lack of exposure of epiblast cells to Wnt signals permits FGFs to induce a neural fate...
  44. ncbi Dickkopf-1 is a member of a new family of secreted proteins and functions in head induction
    A Glinka
    Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Heidelberg, Germany
    Nature 391:357-62. 1998
    ..Injections of mRNA and antibody indicate that dkk-1 is sufficient and necessary to cause head induction. dkk-1 s a potent antagonist of Wnt signalling, suggesting that dkk genes encode a family of secreted Wnt inhibitors...
  45. doi Serotonin signaling is required for Wnt-dependent GRP specification and leftward flow in Xenopus
    Tina Beyer
    Institute of Zoology, University of Hohenheim, Garbenstrasse 30, 70593 Stuttgart, Germany
    Curr Biol 22:33-9. 2012
    ..Our data confirm flow as primary mechanism of symmetry breakage and suggest a general role of serotonin as competence factor for Wnt signaling during axis formation in Xenopus...
  46. ncbi Two novel nodal-related genes initiate early inductive events in Xenopus Nieuwkoop center
    S Takahashi
    Department of Life Sciences Biology, The University of Tokyo, Tokyo 153 8902, Japan
    Development 127:5319-29. 2000
    ..The expression of Xnr5 and Xnr6 is regulated by maternal VegT and (beta)-catenin, but does not require TGF-(beta) signaling. Therefore, expression of Xnr5 and Xnr6 is controlled by different mechanisms from other Xnr family genes...
  47. ncbi Kinesin II mediates Vg1 mRNA transport in Xenopus oocytes
    J Nicholas Betley
    Department of Biology, Boston University, Boston, MA, USA
    Curr Biol 14:219-24. 2004
    ..These data support a model in which kinesin II mediates the transport of specific RNA complexes destined for the vegetal cortex...
  48. doi NLS-mediated NPC functions of the nucleoporin Pom121
    Sevil Yavuz
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    FEBS Lett 584:3292-8. 2010
    ..We propose that the NLS sites of Pom121 affect its function in NPC assembly both by influencing nucleoporin interactions and pore membrane structure...
  49. pmc Xenopus DNA2 is a helicase/nuclease that is found in complexes with replication proteins And-1/Ctf4 and Mcm10 and DSB response proteins Nbs1 and ATM
    Karen E Wawrousek
    Division of Biology, California Institute of Technology, Pasadena, CA, USA
    Cell Cycle 9:1156-66. 2010
    ..The nuclease activity of Mre11 is required, since its inhibition delays both full Dna2 recruitment and resection. Dna2 depletion inhibits but does not block resection, and Chk1 and Chk2 induction occurs in the absence of Dna2...
  50. ncbi Dynamic patterns of gene expression in the developing pronephros of Xenopus laevis
    T J Carroll
    Department of Zoology, University of Texas, Austin 78712, USA
    Dev Genet 24:199-207. 1999
    ..We describe restrictions of these gene expression patterns within the pronephros which indicate a model for the partitioning of the common pronephric anlage into its three component parts--the tubules, the glomus, and the duct...
  51. ncbi Crystal structure of a beta-catenin/Tcf complex
    T A Graham
    Department of Biological Structure University of Washington 98195, Seattle, WA, USA
    Cell 103:885-96. 2000
    ..The structural and mutagenesis data reveal a potential target for molecular drug design studies...
  52. pmc H,K-ATPase protein localization and Kir4.1 function reveal concordance of three axes during early determination of left-right asymmetry
    Sherry Aw
    Forsyth Institute, Forsyth Center for Regenerative and Developmental Biology, 140 The Fenway, Boston, MA 02115, USA
    Mech Dev 125:353-72. 2008
    ....
  53. pmc Convergence of a head-field selector Otx2 and Notch signaling: a mechanism for lens specification
    Hajime Ogino
    Department of Biology, University of Virginia, Charlottesville, VA 22904, USA
    Development 135:249-58. 2008
    ..This concept is also consistent with classical embryological studies of many organ systems involving a ;multiple-step induction'...
  54. pmc Temporal profiling of the chromatin proteome reveals system-wide responses to replication inhibition
    Guennadi A Khoudoli
    Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee, Dundee DD1 5EH, United Kingdom
    Curr Biol 18:838-43. 2008
    ....
  55. ncbi Structural basis of Aurora-A activation by TPX2 at the mitotic spindle
    Richard Bayliss
    Structural and Computational Biology Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117, Heidelberg, Germany
    Mol Cell 12:851-62. 2003
    ..The recognition between Aurora-A and TPX2 resembles that between the cAPK catalytic core and its flanking regions, suggesting this molecular mechanism may be a recurring theme in kinase regulation...
  56. ncbi Wnt5a and Wnt11 interact in a maternal Dkk1-regulated fashion to activate both canonical and non-canonical signaling in Xenopus axis formation
    Sang wook Cha
    Division of Developmental Biology, Cincinnati Children s Research Foundation, Cincinnati, OH 45229, USA
    Development 135:3719-29. 2008
    ..We determine that Wnt5a and Wnt11 form a previously unrecognized complex. This work suggests a new aspect of Wnt signaling: two Wnts acting in a complex together to regulate embryonic patterning...
  57. ncbi Beta-catenin/Tcf-regulated transcription prior to the midblastula transition
    Jing Yang
    Department of Medicine Hematology Oncology and Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Development 129:5743-52. 2002
    ....
  58. pmc Cdt1 downregulation by proteolysis and geminin inhibition prevents DNA re-replication in Xenopus
    Anatoliy Li
    Wellcome Trust Biocentre, University of Dundee, Dundee, UK
    EMBO J 24:395-404. 2005
    ..The results also explain the original experiments that led to the proposal of a replication licensing factor...
  59. ncbi Serotonin transporter function is an early step in left-right patterning in chick and frog embryos
    Takahiro Fukumoto
    Cytokine Biology Department, The Forsyth Institute, Harvard Medical School, Boston, Mass 02115, USA
    Dev Neurosci 27:349-63. 2005
    ..Taken together our data characterize two new right-sided markers in chick gastrulation, identify a novel, early component of the left-right pathway in two vertebrate species, and reveal a new biological role for serotonin transport...
  60. pmc Expression cloning of TMEM16A as a calcium-activated chloride channel subunit
    Björn Christian Schroeder
    Department of Physiology, Howard Hughes Medical Institute, University of California San Francisco, San Francisco, CA 94143, USA
    Cell 134:1019-29. 2008
    ..The identification of this new family of ion channels may help the development of CaCC modulators for treating diseases including hypertension and cystic fibrosis...
  61. pmc The posteriorizing gene Gbx2 is a direct target of Wnt signalling and the earliest factor in neural crest induction
    Bo Li
    Department of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK
    Development 136:3267-78. 2009
    ..We propose a new genetic cascade that operates in the distinction between anterior placodal and NC territories...
  62. pmc Sfrp5 coordinates foregut specification and morphogenesis by antagonizing both canonical and noncanonical Wnt11 signaling
    Yan Li
    Cincinnati Children s Research Foundation and Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, Ohio 45229, USA
    Genes Dev 22:3050-63. 2008
    ..This novel mechanism coordinating canonical and noncanonical Wnt signaling may have broad implications for organogenesis and cancer...
  63. ncbi Neural crest induction in Xenopus: evidence for a two-signal model
    C LaBonne
    Division of Biology, Beckman Institute 139 74, California Institute of Technology, Pasadena, CA 91125, USA
    Development 125:2403-14. 1998
    ..Slug-expressing ectoderm will generate neural crest in the presence of Wnt or FGF-like signals, however, bypassing the need for BMP inhibition in this process. A two-step model for neural crest induction is proposed...
  64. pmc Sequential expression of multiple POU proteins during amphibian early development
    C S Hinkley
    Department of Biochemistry and Molecular Biology, University of Texas M D Anderson Cancer Center, Houston 77030
    Mol Cell Biol 12:638-49. 1992
    ..By analogy with other members of the POU-domain gene family, the products of these genes may play critical roles in the determination of cell fate and the regulation of cell proliferation...
  65. ncbi Expression of Pax-3 in the lateral neural plate is dependent on a Wnt-mediated signal from posterior nonaxial mesoderm
    A G Bang
    Molecular Neurobiology Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California, 92037, USA
    Dev Biol 212:366-80. 1999
    ....
  66. pmc Identification of a novel dynein binding domain in nudel essential for spindle pole organization in Xenopus egg extract
    Shusheng Wang
    Department of Embryology, Carnegie Institution for Science and Howard Hughes Medical Institute, Baltimore, Maryland 21218, USA
    J Biol Chem 286:587-93. 2011
    ..Together, our findings open up new avenues to further decipher the mechanism of dynein regulation by Nudel and Lis1...
  67. ncbi Overexpression of the transcriptional repressor FoxD3 prevents neural crest formation in Xenopus embryos
    B S Pohl
    Abteilung Biochemie, Universitat Ulm, Albert Einstein Allee 11, D 89081 Ulm, Germany
    Mech Dev 103:93-106. 2001
    ..Transplantation experiments show that FoxD3 overexpressing cells from the prospective neural crest do neither differentiate nor migrate...
  68. ncbi Constant regulation of both the MPF amplification loop and the Greatwall-PP2A pathway is required for metaphase II arrest and correct entry into the first embryonic cell cycle
    Thierry Lorca
    Universités Montpellier 2 et 1, Centre de Recherche de Biochimie Macromoleculaire, CNRS UMR 5237, IFR 122, 1919 Route de Mende, 34293 Montpellier Cedex 5, France
    J Cell Sci 123:2281-91. 2010
    ....
  69. ncbi GBP, an inhibitor of GSK-3, is implicated in Xenopus development and oncogenesis
    C Yost
    Department of Biochemistry, University of Washington, Seattle 98195 7350, USA
    Cell 93:1031-41. 1998
    ..Our results define a family of GSK-3-binding proteins with roles in development and cell proliferation...
  70. ncbi Conserved requirement of Lim1 function for cell movements during gastrulation
    Neil A Hukriede
    Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Dev Cell 4:83-94. 2003
    ..We conclude that a primary function of Lim1 in the early embryo is to enable proper cell movements during gastrulation...
  71. pmc Dishevelled controls apical docking and planar polarization of basal bodies in ciliated epithelial cells
    Tae Joo Park
    Department of Molecular Cell and Developmental Biology and Institute for Cellular and Molecular Biology, University of Texas, Austin, Texas 78712, USA
    Nat Genet 40:871-9. 2008
    ..These results demonstrate previously undescribed functions for PCP signaling components and suggest that a common signaling apparatus governs both apical docking and planar polarization of basal bodies...
  72. ncbi The Rad9-Hus1-Rad1 checkpoint clamp regulates interaction of TopBP1 with ATR
    Joon Lee
    Division of Biology, California Institute of Technology, Pasadena, California 91125, USA
    J Biol Chem 282:28036-44. 2007
    ..These findings suggest that interaction of the 9-1-1 complex with the BRCT I-II region of TopBP1 is necessary for binding of ATR-ATRIP to the ATR-activating domain of TopBP1 and the ensuing activation of ATR...
  73. ncbi Induction of the Xenopus organizer: expression and regulation of Xnot, a novel FGF and activin-regulated homeo box gene
    G von Dassow
    Department of Biochemistry, University of Washington, Seattle 98195
    Genes Dev 7:355-66. 1993
    ..Our results provide experimental evidence supporting the proposed division of Spemann's organizer into independently regulated organizing centers...
  74. pmc Xenopus Zic3, a primary regulator both in neural and neural crest development
    K Nakata
    Molecular Neurobiology Laboratory, Tsukuba Life Science Center, Institute of Physical and Chemical Research RIKEN, Tsukuba, Ibaraki 305, Japan
    Proc Natl Acad Sci U S A 94:11980-5. 1997
    ..These findings suggest that Zic3 can determine the ectodermal cell fate and promote the earliest step of neural and neural crest development...
  75. pmc Requirement for Nudel and dynein for assembly of the lamin B spindle matrix
    Li Ma
    Laboratory of Molecular Cell Biology and Center of Cell Signaling, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
    Nat Cell Biol 11:247-56. 2009
    ..We suggest that Nudel regulates microtubule organization in part by facilitating assembly of the lamin B spindle matrix in a dynein-dependent manner...
  76. ncbi Requirement of FoxD3-class signaling for neural crest determination in Xenopus
    N Sasai
    Department of Medical Embryology and Neurobiology, and Department of Molecular and Cellular Biology, Institute for Frontier Medical Sciences, Kyoto University, Sakyo, Kyoto 606 8507, Japan
    Development 128:2525-36. 2001
    ..These data demonstrate that FoxD3 (or its closely related factor) is an essential upstream regulator of neural crest determination...
  77. ncbi FoxN3 is required for craniofacial and eye development of Xenopus laevis
    Maximilian Schuff
    Abteilung Biochemie, Universitat Ulm, Ulm, Germany
    Dev Dyn 236:226-39. 2007
    ....
  78. ncbi Nuclear exclusion of Smad2 is a mechanism leading to loss of competence
    Oliver H Grimm
    Wellcome Trust Cancer Research UK Institute, Tennis Court Road, University of Cambridge, Cambridge, CB2 1QR, UK
    Nat Cell Biol 4:519-22. 2002
    ..This mechanism operates within less than an hour, and is therefore well suited to control an orderly sequence of inductions...
  79. pmc The Xenopus Xmus101 protein is required for the recruitment of Cdc45 to origins of DNA replication
    Ruth A Van Hatten
    Department of Molecular and Cellular Biology, The Biological Laboratories, Harvard University, Cambridge, MA 02138, USA
    J Cell Biol 159:541-7. 2002
    ....
  80. ncbi Cooperative interaction of Xvent-2 and GATA-2 in the activation of the ventral homeobox gene Xvent-1B
    Henner Friedle
    Abteilung Biochemie, Universitat Ulm, Albert Einstein Allee 11, Ulm 89081, Germany
    J Biol Chem 277:23872-81. 2002
    ..This inhibitor is likely a component of the dorsal Wnt signaling pathway because nuclear translocation of beta-catenin before midblastula transition results in a suppression of Xvent-1B transcription...
  81. ncbi CPEB is a specificity factor that mediates cytoplasmic polyadenylation during Xenopus oocyte maturation
    L E Hake
    Worcester Foundation for Experimental Biology Shrewsbury, Massachusetts 01545
    Cell 79:617-27. 1994
    ..Partial restoration of polyadenylation in depleted extracts is achieved by the addition of CPEB, thus demonstrating that this protein is required for cytoplasmic polyadenylation...
  82. pmc BMP inhibition initiates neural induction via FGF signaling and Zic genes
    Leslie Marchal
    Institut de Biologie du Developpement de Marseille Luminy, UMR 6216, CNRS Université de la Méditerranée, 13288 Marseille Cedex 09, France
    Proc Natl Acad Sci U S A 106:17437-42. 2009
    ..We propose to merge the previous models into a unique one whereby neural induction is controlled by BMP inhibition, which activates directly, and, via FGF instructive activity, early neural regulators such as Zic genes...
  83. ncbi Requirement of prorenin receptor and vacuolar H+-ATPase-mediated acidification for Wnt signaling
    Cristina Maria Cruciat
    Division of Molecular Embryology, DKFZ ZMBH Alliance, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
    Science 327:459-63. 2010
    ..The results reveal an unsuspected role for the prorenin receptor, V-ATPase activity, and acidification during Wnt/beta-catenin signaling...
  84. ncbi Expression of Xenopus tropicalis noggin1 and noggin2 in early development: two noggin genes in a tetrapod
    Russell B Fletcher
    Division of Genetics and Development, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    Gene Expr Patterns 5:225-30. 2004
    ..rerio with expression in the forebrain, hindbrain, and somites, but unlike D. rerio, X. tropicalis noggin2 is expressed in the heart by stage 28. This work presents the first example of a tetrapod with at least two noggin genes...
  85. ncbi Overlapping expression of Xwnt-3A and Xwnt-1 in neural tissue of Xenopus laevis embryos
    S L Wolda
    Department of Pharmacology, University of Washington School of Medicine, Seattle 98195
    Dev Biol 155:46-57. 1993
    ..Last, the ectopic expression of Xwnt-3A induces the formation of a secondary axis at the anterior end of the embryo...
  86. pmc Dissection of CENP-C-directed centromere and kinetochore assembly
    Kirstin J Milks
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA
    Mol Biol Cell 20:4246-55. 2009
    ..We find that the amino terminus of CENP-C promotes kinetochore assembly by ensuring proper targeting of the Mis12/MIND complex and CENP-K...
  87. pmc Chromatin immunoprecipitation in early Xenopus laevis embryos
    Shelby A Blythe
    Cell and Molecular Biology Graduate Group, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA
    Dev Dyn 238:1422-32. 2009
    ..Developmental Dynamics 238:1422-1432, 2009. (c) 2009 Wiley-Liss, Inc...
  88. ncbi Xenopus Smad3 is specifically expressed in the chordoneural hinge, notochord and in the endocardium of the developing heart
    M Howell
    Laboratory of Developmental Signalling, Imperial Cancer Research Fund, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Mech Dev 104:147-50. 2001
    ..The chordoneural hinge staining persists at least until stage 40 whereas the staining in the endocardium peaks at approximately stage 32/33...
  89. ncbi New B-type cyclin synthesis is required between meiosis I and II during Xenopus oocyte maturation
    H Hochegger
    ICRF Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, UK
    Development 128:3795-807. 2001
    ..We discuss the interdependence of c-mos and MPF, and reveal an important role for translational control of cyclin synthesis between the two meiotic divisions...
  90. ncbi Frizzled-10, up-regulated in primary colorectal cancer, is a positive regulator of the WNT - beta-catenin - TCF signaling pathway
    Harumi Terasaki
    Laboratory of Molecular Embryology, Graduate School of Science, University of Tokyo, Tokyo 113 0033, Japan
    Int J Mol Med 9:107-12. 2002
    ..These results suggest that up-regulation of FZD10 mRNA in several types of human cells might lead to carcinogenesis through activation of the beta-catenin - TCF signaling pathway synergistically with some class of WNTs...
  91. pmc Replication protein A promotes 5'-->3' end processing during homology-dependent DNA double-strand break repair
    Hong Yan
    Fox Chase Cancer Center, Philadelphia, PA 19111, USA
    J Cell Biol 192:251-61. 2011
    ..These results provide strong biochemical evidence to link RPA to a specific DSB repair pathway and reveal a novel function of RPA in the generation of 3' ss-DNA for homology-dependent DSB repair...
  92. ncbi In vitro induction of the pronephric duct in Xenopus explants
    Kenji Osafune
    Department of Life Sciences Biology, Graduate School of Arts and Sciences, University of Tokyo, 3 8 1 Komaba, Meguro ku, Tokyo 153 8902, Japan
    Dev Growth Differ 44:161-7. 2002
    ....
  93. ncbi Activin/nodal responsiveness and asymmetric expression of a Xenopus nodal-related gene converge on a FAST-regulated module in intron 1
    S I Osada
    Department of Cell Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 2175, USA
    Development 127:2503-14. 2000
    ..Thus, an evolutionarily conserved intronic enhancer in Xnr1 is involved in both mesendoderm induction and asymmetric expression during left-right axis formation...
  94. ncbi Human replication proteins hCdc21, hCdc46 and P1Mcm3 bind chromatin uniformly before S-phase and are displaced locally during DNA replication
    T Krude
    Wellcome CRC Institute, Cambridge, UK
    J Cell Sci 109:309-18. 1996
    ....
  95. ncbi LDL-receptor-related protein 6 is a receptor for Dickkopf proteins
    B Mao
    Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Heidelberg, Germany
    Nature 411:321-5. 2001
    ..Thus, DKKs inhibit Wnt co-receptor function, exemplifying the modulation of LRP signalling by antagonists...
  96. ncbi Regulation of Msx genes by a Bmp gradient is essential for neural crest specification
    Celeste Tribulo
    Millennium Nucleus in Developmental Biology, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile
    Development 130:6441-52. 2003
    ..We propose a model where a gradient of Bmp activity specifies the expression of Msx genes in the neural folds, and that this expression is essential for the early specification of the neural crest...
  97. ncbi Distinct enhancer elements control Hex expression during gastrulation and early organogenesis
    T A Rodriguez
    Division of Mammalian Development, National Institute for Medical Research, The Ridgeway, Mill Hill, London, NW7 1AA, United Kingdom
    Dev Biol 234:304-16. 2001
    ..This analysis also reveals that the initiation of Hex expression in the anterior visceral endoderm and axial mesendoderm requires common elements, but maintenance of expression is regulated independently in these tissues...
  98. ncbi Cardiac septal and valvular dysmorphogenesis in mice heterozygous for mutations in the homeobox gene Nkx2-5
    C Biben
    Victor Chang Cardiac Research Institute, Darlinghurst, Australia
    Circ Res 87:888-95. 2000
    ..Our data demonstrate that the complex effects of Nkx2-5 haploinsufficiency in mice are weaker but convergent with those in humans. As in the mouse, the phenotype of human NKX2-5 mutations may be modulated by interacting alleles...
  99. pmc Translational control by cytoplasmic polyadenylation in Xenopus oocytes
    Helois E Radford
    School of Pharmacy, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, UK
    Biochim Biophys Acta 1779:217-29. 2008
    ..Finally we discuss some of the remaining questions regarding the mechanisms of translational regulation by cytoplasmic polyadenylation and give our view on where our knowledge is likely to be expanded in the near future...
  100. ncbi Tome-1, a trigger of mitotic entry, is degraded during G1 via the APC
    Nagi G Ayad
    Department of Cell Biology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Cell 113:101-13. 2003
    ..Degradation of Tome-1 during G1 allows for wee 1 accumulation during interphase, thereby providing a critical link between the APC and SCF pathways in regulation of cdk1/cyclin B activity and thus mitotic entry and exit...
  101. ncbi Identification of neurogenin, a vertebrate neuronal determination gene
    Q Ma
    Division of Biology, Howard Hughes Medical Institute, California Institute of Technology, Pasadena 91125, USA
    Cell 87:43-52. 1996
    ..The properties of X-NGNR-1 are thus analogous to those of the Drosophila proneural genes, suggesting that it functions as a vertebrate neuronal determination factor...

Research Grants108 found, 100 shown here

  1. MARCKS Phosphorylation in Growth Cone Signaling
    JESSE GATLIN; Fiscal Year: 2004
    ..Functional consequences of aberrant PKC signaling will be determined by observing the turning response of growth cones from DRG neurons expressing a dominant-negative, phosphorylation-deficient MARCKS mutant. ..
  2. Computation tools for analyzing gene expression data
    Naama Barkai; Fiscal Year: 2004
    ..Kinetic microarray experiments that highlight specific features of the transcriptional network will be designed, performed and analyzed. Methods for extracting regulatory relationships from these experiments will be developed. ..
  3. THE ROLE OF MAP KINASE IN THE CELL CYCLE
    JAMES FERRELL; Fiscal Year: 2005
    ..4. To examine the systems-level properties of a p42 MAPK cascade reconstituted with recombinant Xenopus proteins.Our overarching aim is to better understand the cell biology and biochemistry of oocyte maturation and early ..
  4. Intrinsic Spindle Mechanisms to Insure Bipolarity
    JESSE GATLIN; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  5. MOLECULAR GENETICS OF EARLY NEUROGENESIS
    Ethan Bier; Fiscal Year: 2007
    ..These studies may provide the framework for our future studies relevant to human disease and drug abuse. [unreadable] [unreadable] [unreadable]..
  6. BIOLOGICAL FUNCTIONS OF TOPOISOMERASE I IN S CEREVISIAE
    Michael Christman; Fiscal Year: 2000
    ..The SMC family of proteins are related to Xenopus proteins that are required for MCC in viro, suggesting that the mechanism of MCC is evolutionarity conserved...
  7. BIOLOGICAL FUNCTIONS OF TOPOISOMERASE I IN S CEREVISIAE
    Michael Christman; Fiscal Year: 1999
    ..The SMC family of proteins are related to Xenopus proteins that are required for MCC in viro, suggesting that the mechanism of MCC is evolutionarity conserved...
  8. METHYLATION OF ATYPICAL PROTEIN ASPARTYL RESIDUES
    CLARE O CONNOR; Fiscal Year: 1990
    ..In these experiments Xenopus proteins will be pulse-labeled with either L-[C14] aspartic acid or L[14C] asparagine, and the production of ..
  9. THE ROLE OF MAP KINASE IN THE CELL CYCLE
    JAMES FERRELL; Fiscal Year: 2002
    ..4. To examine the systems-level properties of a p42 MAPK cascade reconstituted with recombinant Xenopus proteins.Our overarching aim is to better understand the cell biology and biochemistry of oocyte maturation and early ..
  10. THE ROLE OF MAP KINASE IN THE CELL CYCLE
    JAMES FERRELL; Fiscal Year: 2002
    ..4. To examine the systems-level properties of a p42 MAPK cascade reconstituted with recombinant Xenopus proteins.Our overarching aim is to better understand the cell biology and biochemistry of oocyte maturation and early ..
  11. THE ROLE OF MAP KINASE IN THE CELL CYCLE
    JAMES FERRELL; Fiscal Year: 2004
    ..4. To examine the systems-level properties of a p42 MAPK cascade reconstituted with recombinant Xenopus proteins.Our overarching aim is to better understand the cell biology and biochemistry of oocyte maturation and early ..
  12. THE ROLE OF MAP KINASE IN THE CELL CYCLE
    JAMES FERRELL; Fiscal Year: 2003
    ..4. To examine the systems-level properties of a p42 MAPK cascade reconstituted with recombinant Xenopus proteins.Our overarching aim is to better understand the cell biology and biochemistry of oocyte maturation and early ..
  13. EUKARYOTIC HYALURONAN SYNTHASES
    PAUL DEANGELIS; Fiscal Year: 1999
    ..The essential residues identified in aim 3 will also be confirmed by mutagenesis studies. The enzymological and structural information may allow the generation of a working model describing the catalytic mechanism of these enzymes. ..
  14. TRAINING IN MOLECULAR AND CELLULAR BIOLOGY
    David Kimelman; Fiscal Year: 2007
    ..The trainees have a strong record of success as shown by their publications in graduate school and the career choices they make after they leave graduate school. ..
  15. MOLECULAR REGULATION OF EARLY XENOPUS DEVELOPMENT
    David Kimelman; Fiscal Year: 2003
    ..Since the Wnt pathway is implicated in normal vertebrate development and oncogenesis, these studies will be important in understanding the molecular basis of birth defects and cancer. ..
  16. Wnt Pathway Regulation in Embryos and Cells
    David Kimelman; Fiscal Year: 2007
    ..Since the Wnt pathway plays key roles in vertebrate development and oncogenesis, these studies will be important in advancing knowledge of the molecular basis of birth defects and cancer. ..
  17. EUKARYOTIC HYALURONAN SYNTHASES
    PAUL DEANGELIS; Fiscal Year: 2000
    ..The essential residues identified in aim 3 will also be confirmed by mutagenesis studies. The enzymological and structural information may allow the generation of a working model describing the catalytic mechanism of these enzymes. ..
  18. MOLECULAR REGULATION OF EARLY XENOPUS DEVELOPMENT
    David Kimelman; Fiscal Year: 2000
    ..Since the Wnt pathway is implicated in normal vertebrate development and oncogenesis, these studies will be important in understanding the molecular basis of birth defects and cancer. ..
  19. Wnt Pathway Regulation in Embryos and Cells
    David Kimelman; Fiscal Year: 2006
    ..Since the Wnt pathway plays key roles in vertebrate development and oncogenesis, these studies will be important in advancing knowledge of the molecular basis of birth defects and cancer. ..
  20. Combinatorial signaling in zebrafish development
    David Kimelman; Fiscal Year: 2005
    ..abstract_text> ..
  21. MOLECULAR REGULATION OF EARLY XENOPUS DEVELOPMENT
    David Kimelman; Fiscal Year: 2001
    ..Since the Wnt pathway is implicated in normal vertebrate development and oncogenesis, these studies will be important in understanding the molecular basis of birth defects and cancer. ..
  22. EUKARYOTIC HYALURONAN SYNTHASES
    PAUL DEANGELIS; Fiscal Year: 2001
    ..The essential residues identified in aim 3 will also be confirmed by mutagenesis studies. The enzymological and structural information may allow the generation of a working model describing the catalytic mechanism of these enzymes. ..
  23. Wnt Pathway Regulation in Embryos and Cells
    David Kimelman; Fiscal Year: 2005
    ..Since the Wnt pathway plays key roles in vertebrate development and oncogenesis, these studies will be important in advancing knowledge of the molecular basis of birth defects and cancer. ..
  24. MOLECULAR REGULATION OF EARLY XENOPUS DEVELOPMENT
    David Kimelman; Fiscal Year: 2002
    ..Since the Wnt pathway is implicated in normal vertebrate development and oncogenesis, these studies will be important in understanding the molecular basis of birth defects and cancer. ..
  25. TRAINING IN MOLECULAR AND CELLULAR BIOLOGY
    David Kimelman; Fiscal Year: 2008
    ..The trainees have a strong record of success as shown by their publications in graduate school and the career choices they make after they leave graduate school. ..
  26. Regulation of mesodermal progenitors in transgenic zebrafish
    David Kimelman; Fiscal Year: 2009
    ....
  27. Regulation of mesodermal progenitors in transgenic zebrafish
    David Kimelman; Fiscal Year: 2010
    ....
  28. Regulation of mesodermal progenitors in transgenic zebrafish
    David Kimelman; Fiscal Year: 2009
    ....
  29. Wnt Pathway Regulation in Embryos and Cells
    David Kimelman; Fiscal Year: 2009
    ..Since the Wnt pathway plays key roles in vertebrate development and oncogenesis, these studies will be important in advancing knowledge of the molecular basis of birth defects and cancer. ..
  30. Regulation of mesodermal progenitors in transgenic zebrafish
    David Kimelman; Fiscal Year: 2008
    ....
  31. Wnt Pathway Regulation in Embryos and Cells
    David Kimelman; Fiscal Year: 2008
    ..Since the Wnt pathway plays key roles in vertebrate development and oncogenesis, these studies will be important in advancing knowledge of the molecular basis of birth defects and cancer. ..
  32. MOLECULAR REGULATION OF EARLY XENOPUS DEVELOPMENT
    David Kimelman; Fiscal Year: 1999
    ..Since the Wnt pathway is implicated in normal vertebrate development and oncogenesis, these studies will be important in understanding the molecular basis of birth defects and cancer. ..
  33. MOLECULAR REGULATION OF EARLY XENOPUS DEVELOPMENT
    David Kimelman; Fiscal Year: 1993
    ..Since bFGF s not only involved in development regulation but can also convert common issues in different medical fields...
  34. MOLECULAR REGULATION OF EARLY XENOPUS DEVELOPMENT
    David Kimelman; Fiscal Year: 1992
    ..Since bFGF s not only involved in development regulation but can also convert common issues in different medical fields...
  35. MOLECULAR REGULATION OF EARLY XENOPUS DEVELOPMENT
    David Kimelman; Fiscal Year: 1991
    ..Since bFGF s not only involved in development regulation but can also convert common issues in different medical fields...
  36. Cytoskeleton polymerization dynamics in the cell cycle
    Timothy Mitchison; Fiscal Year: 2007
    ..Understanding midbody assembles will reveal principles for generating asymmetric assemblies in the cytoplasm, and will tell us how the cleavage furrow is positioned. ..
  37. Biochemistry and Cytology of Cytokinesis
    Timothy Mitchison; Fiscal Year: 2003
    ..We will probe how the microtubules associated with cytokinesis are organized in the cell and what mechanisms they use to target anillin and myosin II to the center of the cell to build a furrow there. ..
  38. ELECTRON MICROSCOPE
    Timothy Mitchison; Fiscal Year: 2004
    ..The automated controls and digital image acquisition will also be optimal for introducing new users to electron microscopy and for minimizing maintenance requirements that might arise from operator inexperience. ..
  39. Cytoskeleton polymerization dynamics in the cell cycle
    Timothy Mitchison; Fiscal Year: 2008
    ..Understanding midbody assembles will reveal principles for generating asymmetric assemblies in the cytoplasm, and will tell us how the cleavage furrow is positioned. ..
  40. MICROTUBULE DYNAMICS AND MITOTIC MECHANISM
    Timothy Mitchison; Fiscal Year: 2008
    ..These discoveries will have immediate relevance to the development of novel cancer drugs. ..
  41. ACTIN DYNAMICS AND CELL LOCOMOTION
    Timothy Mitchison; Fiscal Year: 2004
    ..For perturbation experiments we will use protein tools, and also cell permeable chemicals coming out of a screening effort at the Harvard Institute of Chemistry and Cell Biology (ICCB). ..
  42. Cytoskeleton polymerization dynamics in the cell cycle
    Timothy J Mitchison; Fiscal Year: 2010
    ..Understanding midbody assembles will reveal principles for generating asymmetric assemblies in the cytoplasm, and will tell us how the cleavage furrow is positioned. ..
  43. MICROTUBULE DYNAMICS AND MITOTIC MECHANISM
    Timothy J Mitchison; Fiscal Year: 2010
    ..These discoveries will have immediate relevance to the development of novel cancer drugs. ..
  44. Cytoskeleton polymerization dynamics in the cell cycle
    Timothy Mitchison; Fiscal Year: 2009
    ..Understanding midbody assembles will reveal principles for generating asymmetric assemblies in the cytoplasm, and will tell us how the cleavage furrow is positioned. ..
  45. Biochemistry and Cytology of Cytokinesis
    Timothy Mitchison; Fiscal Year: 2006
    ..We will probe how the microtubules associated with cytokinesis are organized in the cell and what mechanisms they use to target anillin and myosin II to the center of the cell to build a furrow there. ..
  46. MICROTUBULE DYNAMICS AND MITOTIC MECHANISM
    Timothy Mitchison; Fiscal Year: 2007
    ..These discoveries will have immediate relevance to the development of novel cancer drugs. ..
  47. Biochemistry and Cytology of Cytokinesis
    Timothy Mitchison; Fiscal Year: 2005
    ..We will probe how the microtubules associated with cytokinesis are organized in the cell and what mechanisms they use to target anillin and myosin II to the center of the cell to build a furrow there. ..
  48. ACTIN DYNAMICS AND CELL LOCOMOTION
    Timothy Mitchison; Fiscal Year: 2001
    ..For perturbation experiments we will use protein tools, and also cell permeable chemicals coming out of a screening effort at the Harvard Institute of Chemistry and Cell Biology (ICCB). ..
  49. ACTIN DYNAMICS AND CELL LOCOMOTION
    Timothy Mitchison; Fiscal Year: 2000
    ..They then intend to use what they learn in Listeria and apply it to understanding the movement of whole cells. One focus will be the mechanism by which the filopodia of neuronal growth cones extend and retract. ..
  50. GENETIC AND BIOCHEMICAL ANALYSIS OF CYTOKINESIS
    Timothy Mitchison; Fiscal Year: 2000
    ....
  51. ACTIN DYNAMICS AND CELL LOCOMOTION
    Timothy Mitchison; Fiscal Year: 1999
    ..They then intend to use what they learn in Listeria and apply it to understanding the movement of whole cells. One focus will be the mechanism by which the filopodia of neuronal growth cones extend and retract. ..
  52. GENETIC AND BIOCHEMICAL ANALYSIS OF CYTOKINESIS
    Timothy Mitchison; Fiscal Year: 1999
    ....
  53. ACTIN DYNAMICS AND CELL LOCOMOTION
    Timothy Mitchison; Fiscal Year: 2002
    ..For perturbation experiments we will use protein tools, and also cell permeable chemicals coming out of a screening effort at the Harvard Institute of Chemistry and Cell Biology (ICCB). ..
  54. ACTIN DYNAMICS AND CELL LOCOMOTION
    Timothy Mitchison; Fiscal Year: 2003
    ..For perturbation experiments we will use protein tools, and also cell permeable chemicals coming out of a screening effort at the Harvard Institute of Chemistry and Cell Biology (ICCB). ..
  55. Intrinsic Spindle Mechanisms to Insure Bipolarity
    JESSE GATLIN; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  56. Transcriptional regulatory networks controlling Xenopus pancreas development
    MARKO HORB; Fiscal Year: 2008
    ....
  57. Transcriptional regulatory networks controlling Xenopus pancreas development
    MARKO HORB; Fiscal Year: 2009
    ....
  58. Transcriptional regulatory networks controlling Xenopus pancreas development
    MARKO HORB; Fiscal Year: 2007
    ....
  59. 2006 Biophysical Discussions - Molecuar Motors: Point Counterpoint
    Steven Rosenfeld; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  60. Transcriptional regulatory networks controlling Xenopus pancreas development
    MARKO HORB; Fiscal Year: 2009
    ....
  61. Transcriptional regulatory networks controlling Xenopus pancreas development
    Marko E Horb; Fiscal Year: 2010
    ....
  62. Mechanisms of Processivity in Molecular Motors
    Steven Rosenfeld; Fiscal Year: 2008
    ..Furthermore, if successful, this work will support the argument that understanding how a motor works in vitro as an enzyme can provide valuable insights into how it works in vivo in the cell. ..
  63. Mechanisms of Processivity in Molecular Motors
    Steven Rosenfeld; Fiscal Year: 2007
    ..Furthermore, if successful, this work will support the argument that understanding how a motor works in vitro as an enzyme can provide valuable insights into how it works in vivo in the cell. ..
  64. Mechanisms of Processivity in Molecular Motors
    Steven Rosenfeld; Fiscal Year: 2005
    ..Furthermore, if successful, this work will support the argument that understanding how a motor works in vitro as an enzyme can provide valuable insights into how it works in vivo in the cell. ..
  65. Development of High Throughput Screening Assays (RMI)
    Steven Rosenfeld; Fiscal Year: 2004
    ..This work will serve as the basis for a future extramural funding initiative designed to take promising compounds identified by this approach into pre-clinical glioma model systems, and ultimately, into clinical trial. ..
  66. Mechanisms of Processivity in Molecular Motors
    Steven Rosenfeld; Fiscal Year: 2006
    ..Furthermore, if successful, this work will support the argument that understanding how a motor works in vitro as an enzyme can provide valuable insights into how it works in vivo in the cell. ..
  67. Mechanisms of Processivity in Molecular Motors
    Steven Rosenfeld; Fiscal Year: 2005
    ..Furthermore, if successful, this work will support the argument that understanding how a motor works in vitro as an enzyme can provide valuable insights into how it works in vivo in the cell. ..
  68. Mechanisms of Processivity in Molecular Motors
    Steven Rosenfeld; Fiscal Year: 2004
    ..Furthermore, if successful, this work will support the argument that understanding how a motor works in vitro as an enzyme can provide valuable insights into how it works in vivo in the cell. ..
  69. MOLECULAR GENETICS OF EARLY NEUROGENESIS
    Ethan Bier; Fiscal Year: 2008
    ..These studies may provide the framework for our future studies relevant to human disease and drug abuse. ..
  70. Analysis of Early Wing Vein Development in Drosophila
    Ethan Bier; Fiscal Year: 2004
    ..Thus, knowledge gained from these studies should have broad applications to understanding vertebrate development as well as offering possible insights into mechanisms of human disease. ..
  71. ANALYSIS OF EARLY WING VEIN DEVELOPMENT IN DROSPHILIA
    Ethan Bier; Fiscal Year: 2004
    ..Because mis-regulation of these known pathways causes aggressive forms of cancer, understanding the regulation of these pathways also is of significant relevance to cancer. ..
  72. MOLECULAR GENETICS OF EARLY NEUROGENESIS
    Ethan Bier; Fiscal Year: 2004
    ..2) Determine whether different forms of Sog block selected subsets of BMP ligands. 3) Analyze positive acting forms of Sog and determine their range of action. ..
  73. Analyzing Anthrox Toxins in Drosophila
    Ethan Bier; Fiscal Year: 2009
    ..Finally, we will collaborate with other groups to determine whether the LF and EF act similarly in vertebrates. ..
  74. MOLECULAR GENETICS OF EARLY NEUROGENESIS
    Ethan Bier; Fiscal Year: 2009
    ..These studies may provide the framework for our future studies relevant to human disease and drug abuse. ..
  75. MOLECULAR GENETICS OF EARLY NEUROGENESIS
    Ethan Bier; Fiscal Year: 2002
    ..2) Determine whether different forms of Sog block selected subsets of BMP ligands. 3) Analyze positive acting forms of Sog and determine their range of action. ..
  76. MOLECULAR GENETICS OF NEUROGENESIS
    Ethan Bier; Fiscal Year: 1993
    ..g modifiers of the scratched eye phenotype of putative scrt alleles). Finally, we will use low stringency hybridization and PCR amplification with scrt zinc finger probes and primers to search for structurally related pan-neural genes...
  77. ANALYSIS OF EARLY WING VEIN DEVELOPMENT IN DROSPHILIA
    Ethan Bier; Fiscal Year: 2001
    ..Because mis-regulation of these known pathways causes aggressive forms of cancer, understanding the regulation of these pathways also is of significant relevance to cancer. ..
  78. Analysis of Early Wing Vein Development in Drosophila
    Ethan Bier; Fiscal Year: 2006
    ..Thus, knowledge gained from these studies should have broad applications to understanding vertebrate development as well as offering possible insights into mechanisms of human disease. ..
  79. MOLECULAR GENETICS OF EARLY NEUROGENESIS
    Ethan Bier; Fiscal Year: 2000
    ..III. Identify enhancer element(s) directing sog expression during embryonic and adult development. IV. Determine whether a Sog protein(s) is secreted, diffuses, and binds to Dpp. ..
  80. ANALYSIS OF EARLY WING VEIN DEVELOPMENT IN DROSPHILIA
    Ethan Bier; Fiscal Year: 2000
    ..Because mis-regulation of these known pathways causes aggressive forms of cancer, understanding the regulation of these pathways also is of significant relevance to cancer. ..
  81. MOLECULAR GENETICS OF EARLY NEUROGENESIS
    Ethan Bier; Fiscal Year: 2003
    ..2) Determine whether different forms of Sog block selected subsets of BMP ligands. 3) Analyze positive acting forms of Sog and determine their range of action. ..
  82. ANALYSIS OF EARLY WING VEIN DEVELOPMENT IN DROSPHILIA
    Ethan Bier; Fiscal Year: 2001
    ..Because mis-regulation of these known pathways causes aggressive forms of cancer, understanding the regulation of these pathways also is of significant relevance to cancer. ..
  83. Genetic Analysis of Bacterial Toxins in Drosophia
    Ethan Bier; Fiscal Year: 2005
    ..DN alleles 2-2: Characterize molecular lesion in DN-toxin NOVA alleles 2-3: Confirm that DN-toxin alleles interfere with wild-type toxin gene function 2-4: Determine whether DN-toxin alleles function in mammalian cells ..
  84. MOLECULAR GENETICS OF EARLY NEUROGENESIS
    Ethan Bier; Fiscal Year: 2006
    ..These studies may provide the framework for our future studies relevant to human disease and drug abuse. [unreadable] [unreadable] [unreadable]..