xenopus proteins

Summary

Summary: Proteins obtained from various species of Xenopus. Included here are proteins from the African clawed frog (XENOPUS LAEVIS). Many of these proteins have been the subject of scientific investigations in the area of MORPHOGENESIS and development.

Top Publications

  1. ncbi H,K-ATPase protein localization and Kir4.1 function reveal concordance of three axes during early determination of left-right asymmetry
    Sherry Aw
    Forsyth Institute, Forsyth Center for Regenerative and Developmental Biology, 140 The Fenway, Boston, MA 02115, USA
    Mech Dev 125:353-72. 2008
  2. ncbi Crystal structure of a beta-catenin/Tcf complex
    T A Graham
    Department of Biological Structure University of Washington 98195, Seattle, WA, USA
    Cell 103:885-96. 2000
  3. ncbi Serotonin transporter function is an early step in left-right patterning in chick and frog embryos
    Takahiro Fukumoto
    Cytokine Biology Department, The Forsyth Institute, Harvard Medical School, Boston, Mass. 02115, USA
    Dev Neurosci 27:349-63. 2005
  4. ncbi Dynamic patterns of gene expression in the developing pronephros of Xenopus laevis
    T J Carroll
    Department of Zoology, University of Texas, Austin 78712, USA
    Dev Genet 24:199-207. 1999
  5. ncbi Temporal profiling of the chromatin proteome reveals system-wide responses to replication inhibition
    Guennadi A Khoudoli
    Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee, Dundee DD1 5EH, United Kingdom
    Curr Biol 18:838-43. 2008
  6. ncbi Convergence of a head-field selector Otx2 and Notch signaling: a mechanism for lens specification
    Hajime Ogino
    Department of Biology, University of Virginia, Charlottesville, VA 22904, USA
    Development 135:249-58. 2008
  7. ncbi Xenopus DNA2 is a helicase/nuclease that is found in complexes with replication proteins And-1/Ctf4 and Mcm10 and DSB response proteins Nbs1 and ATM
    Karen E Wawrousek
    Division of Biology, California Institute of Technology, Pasadena, CA, USA
    Cell Cycle 9:1156-66. 2010
  8. ncbi Mcm10 and And-1/CTF4 recruit DNA polymerase alpha to chromatin for initiation of DNA replication
    Wenge Zhu
    Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Genes Dev 21:2288-99. 2007
  9. ncbi Dishevelled controls apical docking and planar polarization of basal bodies in ciliated epithelial cells
    Tae Joo Park
    Department of Molecular Cell and Developmental Biology and Institute for Cellular and Molecular Biology, University of Texas, Austin, Texas 78712, USA
    Nat Genet 40:871-9. 2008
  10. ncbi GBP, an inhibitor of GSK-3, is implicated in Xenopus development and oncogenesis
    C Yost
    Department of Biochemistry, University of Washington, Seattle 98195 7350, USA
    Cell 93:1031-41. 1998

Research Grants

  1. MARCKS Phosphorylation in Growth Cone Signaling
    JESSE GATLIN; Fiscal Year: 2004
  2. Computation tools for analyzing gene expression data
    Naama Barkai; Fiscal Year: 2004
  3. THE ROLE OF MAP KINASE IN THE CELL CYCLE
    JAMES FERRELL; Fiscal Year: 2005
  4. Intrinsic Spindle Mechanisms to Insure Bipolarity
    JESSE GATLIN; Fiscal Year: 2007
  5. MOLECULAR GENETICS OF EARLY NEUROGENESIS
    Ethan Bier; Fiscal Year: 2007
  6. TRAINING IN MOLECULAR AND CELLULAR BIOLOGY
    David Kimelman; Fiscal Year: 2007
  7. MOLECULAR REGULATION OF EARLY XENOPUS DEVELOPMENT
    David Kimelman; Fiscal Year: 2003
  8. Combinatorial signaling in zebrafish development
    David Kimelman; Fiscal Year: 2005
  9. EUKARYOTIC HYALURONAN SYNTHASES
    PAUL DEANGELIS; Fiscal Year: 2001
  10. Regulation of mesodermal progenitors in transgenic zebrafish
    David Kimelman; Fiscal Year: 2009

Detail Information

Publications202 found, 100 shown here

  1. ncbi H,K-ATPase protein localization and Kir4.1 function reveal concordance of three axes during early determination of left-right asymmetry
    Sherry Aw
    Forsyth Institute, Forsyth Center for Regenerative and Developmental Biology, 140 The Fenway, Boston, MA 02115, USA
    Mech Dev 125:353-72. 2008
    ....
  2. ncbi Crystal structure of a beta-catenin/Tcf complex
    T A Graham
    Department of Biological Structure University of Washington 98195, Seattle, WA, USA
    Cell 103:885-96. 2000
    ..The structural and mutagenesis data reveal a potential target for molecular drug design studies...
  3. ncbi Serotonin transporter function is an early step in left-right patterning in chick and frog embryos
    Takahiro Fukumoto
    Cytokine Biology Department, The Forsyth Institute, Harvard Medical School, Boston, Mass. 02115, USA
    Dev Neurosci 27:349-63. 2005
    ..Taken together our data characterize two new right-sided markers in chick gastrulation, identify a novel, early component of the left-right pathway in two vertebrate species, and reveal a new biological role for serotonin transport...
  4. ncbi Dynamic patterns of gene expression in the developing pronephros of Xenopus laevis
    T J Carroll
    Department of Zoology, University of Texas, Austin 78712, USA
    Dev Genet 24:199-207. 1999
    ..We describe restrictions of these gene expression patterns within the pronephros which indicate a model for the partitioning of the common pronephric anlage into its three component parts--the tubules, the glomus, and the duct...
  5. ncbi Temporal profiling of the chromatin proteome reveals system-wide responses to replication inhibition
    Guennadi A Khoudoli
    Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee, Dundee DD1 5EH, United Kingdom
    Curr Biol 18:838-43. 2008
    ....
  6. ncbi Convergence of a head-field selector Otx2 and Notch signaling: a mechanism for lens specification
    Hajime Ogino
    Department of Biology, University of Virginia, Charlottesville, VA 22904, USA
    Development 135:249-58. 2008
    ..This concept is also consistent with classical embryological studies of many organ systems involving a ;multiple-step induction'...
  7. ncbi Xenopus DNA2 is a helicase/nuclease that is found in complexes with replication proteins And-1/Ctf4 and Mcm10 and DSB response proteins Nbs1 and ATM
    Karen E Wawrousek
    Division of Biology, California Institute of Technology, Pasadena, CA, USA
    Cell Cycle 9:1156-66. 2010
    ..The nuclease activity of Mre11 is required, since its inhibition delays both full Dna2 recruitment and resection. Dna2 depletion inhibits but does not block resection, and Chk1 and Chk2 induction occurs in the absence of Dna2...
  8. ncbi Mcm10 and And-1/CTF4 recruit DNA polymerase alpha to chromatin for initiation of DNA replication
    Wenge Zhu
    Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Genes Dev 21:2288-99. 2007
    ..The discovery also adds to the connection between replication initiation and sister chromatid cohesion...
  9. ncbi Dishevelled controls apical docking and planar polarization of basal bodies in ciliated epithelial cells
    Tae Joo Park
    Department of Molecular Cell and Developmental Biology and Institute for Cellular and Molecular Biology, University of Texas, Austin, Texas 78712, USA
    Nat Genet 40:871-9. 2008
    ..These results demonstrate previously undescribed functions for PCP signaling components and suggest that a common signaling apparatus governs both apical docking and planar polarization of basal bodies...
  10. ncbi GBP, an inhibitor of GSK-3, is implicated in Xenopus development and oncogenesis
    C Yost
    Department of Biochemistry, University of Washington, Seattle 98195 7350, USA
    Cell 93:1031-41. 1998
    ..Our results define a family of GSK-3-binding proteins with roles in development and cell proliferation...
  11. ncbi Conserved requirement of Lim1 function for cell movements during gastrulation
    Neil A Hukriede
    Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Dev Cell 4:83-94. 2003
    ..We conclude that a primary function of Lim1 in the early embryo is to enable proper cell movements during gastrulation...
  12. ncbi Requirement for Nudel and dynein for assembly of the lamin B spindle matrix
    Li Ma
    Laboratory of Molecular Cell Biology and Center of Cell Signaling, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
    Nat Cell Biol 11:247-56. 2009
    ..We suggest that Nudel regulates microtubule organization in part by facilitating assembly of the lamin B spindle matrix in a dynein-dependent manner...
  13. ncbi Xenopus Zic3, a primary regulator both in neural and neural crest development
    K Nakata
    Molecular Neurobiology Laboratory, Tsukuba Life Science Center, Institute of Physical and Chemical Research RIKEN, Tsukuba, Ibaraki 305, Japan
    Proc Natl Acad Sci U S A 94:11980-5. 1997
    ..These findings suggest that Zic3 can determine the ectodermal cell fate and promote the earliest step of neural and neural crest development...
  14. ncbi Control of beta-catenin phosphorylation/degradation by a dual-kinase mechanism
    Chunming Liu
    Division of Neuroscience, Children s Hospital, Department of Neurology, Harvard Medical School, Boston, MA 02115, USA
    Cell 108:837-47. 2002
    ..Our study uncovers distinct roles and steps of beta-catenin phosphorylation, identifies CKIalpha as a component in Wnt/beta-catenin signaling, and has implications to pathogenesis/therapeutics of human cancers and diabetes...
  15. ncbi The Rad9-Hus1-Rad1 checkpoint clamp regulates interaction of TopBP1 with ATR
    Joon Lee
    Division of Biology, California Institute of Technology, Pasadena, California 91125, USA
    J Biol Chem 282:28036-44. 2007
    ..These findings suggest that interaction of the 9-1-1 complex with the BRCT I-II region of TopBP1 is necessary for binding of ATR-ATRIP to the ATR-activating domain of TopBP1 and the ensuing activation of ATR...
  16. ncbi Induction of the Xenopus organizer: expression and regulation of Xnot, a novel FGF and activin-regulated homeo box gene
    G von Dassow
    Department of Biochemistry, University of Washington, Seattle 98195
    Genes Dev 7:355-66. 1993
    ..Our results provide experimental evidence supporting the proposed division of Spemann's organizer into independently regulated organizing centers...
  17. ncbi XTcf-3 transcription factor mediates beta-catenin-induced axis formation in Xenopus embryos
    M Molenaar
    Department of Immunology University Hospital, Utrecht The Netherlands
    Cell 86:391-9. 1996
    ..We propose that signaling by beta-catenin involves complex formation with XTcf-3, followed by nuclear translocation and activation of specific XTcf-3 target genes...
  18. ncbi Requirement of FoxD3-class signaling for neural crest determination in Xenopus
    N Sasai
    Department of Medical Embryology and Neurobiology, and Department of Molecular and Cellular Biology, Institute for Frontier Medical Sciences, Kyoto University, Sakyo, Kyoto 606 8507, Japan
    Development 128:2525-36. 2001
    ..These data demonstrate that FoxD3 (or its closely related factor) is an essential upstream regulator of neural crest determination...
  19. ncbi Nuclear exclusion of Smad2 is a mechanism leading to loss of competence
    Oliver H Grimm
    Wellcome Trust/Cancer Research UK Institute, Tennis Court Road, University of Cambridge, Cambridge, CB2 1QR, UK
    Nat Cell Biol 4:519-22. 2002
    ..This mechanism operates within less than an hour, and is therefore well suited to control an orderly sequence of inductions...
  20. ncbi The Xenopus Xmus101 protein is required for the recruitment of Cdc45 to origins of DNA replication
    Ruth A Van Hatten
    Department of Molecular and Cellular Biology, The Biological Laboratories, Harvard University, Cambridge, MA 02138, USA
    J Cell Biol 159:541-7. 2002
    ....
  21. ncbi Cooperative interaction of Xvent-2 and GATA-2 in the activation of the ventral homeobox gene Xvent-1B
    Henner Friedle
    Abteilung Biochemie, , Albert-Einstein Allee 11, Ulm 89081, Germany
    J Biol Chem 277:23872-81. 2002
    ..This inhibitor is likely a component of the dorsal Wnt signaling pathway because nuclear translocation of beta-catenin before midblastula transition results in a suppression of Xvent-1B transcription...
  22. ncbi FoxN3 is required for craniofacial and eye development of Xenopus laevis
    Maximilian Schuff
    Abteilung Biochemie, Universitat Ulm, Ulm, Germany
    Dev Dyn 236:226-39. 2007
    ....
  23. ncbi In vitro induction of the pronephric duct in Xenopus explants
    Kenji Osafune
    Department of Life Sciences (Biology, Graduate School of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan
    Dev Growth Differ 44:161-7. 2002
    ....
  24. ncbi Synergism between Pax-8 and lim-1 in embryonic kidney development
    T J Carroll
    Cell and Developmental Biology, University of Texas, Austin, Texas, 78712, USA
    Dev Biol 214:46-59. 1999
    ..Together these data indicate that the interaction between XPax-8 and Xlim-1 is a key early step in the establishment of the pronephric primordium...
  25. ncbi Frizzled-10, up-regulated in primary colorectal cancer, is a positive regulator of the WNT - beta-catenin - TCF signaling pathway
    Harumi Terasaki
    Laboratory of Molecular Embryology, Graduate School of Science, University of Tokyo, Tokyo 113 0033, Japan
    Int J Mol Med 9:107-12. 2002
    ..These results suggest that up-regulation of FZD10 mRNA in several types of human cells might lead to carcinogenesis through activation of the beta-catenin - TCF signaling pathway synergistically with some class of WNTs...
  26. ncbi Activin/nodal responsiveness and asymmetric expression of a Xenopus nodal-related gene converge on a FAST-regulated module in intron 1
    S I Osada
    Department of Cell Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 2175, USA
    Development 127:2503-14. 2000
    ..Thus, an evolutionarily conserved intronic enhancer in Xnr1 is involved in both mesendoderm induction and asymmetric expression during left-right axis formation...
  27. ncbi Expression of Xenopus tropicalis noggin1 and noggin2 in early development: two noggin genes in a tetrapod
    Russell B Fletcher
    Division of Genetics and Development, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    Gene Expr Patterns 5:225-30. 2004
    ..rerio with expression in the forebrain, hindbrain, and somites, but unlike D. rerio, X. tropicalis noggin2 is expressed in the heart by stage 28. This work presents the first example of a tetrapod with at least two noggin genes...
  28. ncbi Xenopus Smad3 is specifically expressed in the chordoneural hinge, notochord and in the endocardium of the developing heart
    M Howell
    Laboratory of Developmental Signalling, Imperial Cancer Research Fund, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Mech Dev 104:147-50. 2001
    ..The chordoneural hinge staining persists at least until stage 40 whereas the staining in the endocardium peaks at approximately stage 32/33...
  29. ncbi New B-type cyclin synthesis is required between meiosis I and II during Xenopus oocyte maturation
    H Hochegger
    ICRF Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, UK
    Development 128:3795-807. 2001
    ..We discuss the interdependence of c-mos and MPF, and reveal an important role for translational control of cyclin synthesis between the two meiotic divisions...
  30. ncbi Overlapping expression of Xwnt-3A and Xwnt-1 in neural tissue of Xenopus laevis embryos
    S L Wolda
    Department of Pharmacology, University of Washington School of Medicine, Seattle 98195
    Dev Biol 155:46-57. 1993
    ..Last, the ectopic expression of Xwnt-3A induces the formation of a secondary axis at the anterior end of the embryo...
  31. ncbi BMP inhibition initiates neural induction via FGF signaling and Zic genes
    Leslie Marchal
    Institut de Biologie du Developpement de Marseille Luminy, UMR 6216, CNRS Université de la Méditerranée, 13288 Marseille Cedex 09, France
    Proc Natl Acad Sci U S A 106:17437-42. 2009
    ..We propose to merge the previous models into a unique one whereby neural induction is controlled by BMP inhibition, which activates directly, and, via FGF instructive activity, early neural regulators such as Zic genes...
  32. ncbi Dissection of CENP-C-directed centromere and kinetochore assembly
    Kirstin J Milks
    Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA
    Mol Biol Cell 20:4246-55. 2009
    ..We find that the amino terminus of CENP-C promotes kinetochore assembly by ensuring proper targeting of the Mis12/MIND complex and CENP-K...
  33. ncbi Replication protein A promotes 5'-->3' end processing during homology-dependent DNA double-strand break repair
    Hong Yan
    Fox Chase Cancer Center, Philadelphia, PA 19111, USA
    J Cell Biol 192:251-61. 2011
    ..These results provide strong biochemical evidence to link RPA to a specific DSB repair pathway and reveal a novel function of RPA in the generation of 3' ss-DNA for homology-dependent DSB repair...
  34. ncbi Biochemical and cellular effects of roscovitine, a potent and selective inhibitor of the cyclin-dependent kinases cdc2, cdk2 and cdk5
    L Meijer
    CNRS, Station Biologique, Roscoff, France
    Eur J Biochem 243:527-36. 1997
    ..Through its unique selectivity for some cyclin-dependent kinases, roscovitine provides a useful antimitotic reagent for cell cycle studies and may prove interesting to control cells with deregulated cdc2, cdk2 or cdk5 kinase activities...
  35. ncbi Identification of a novel dynein binding domain in nudel essential for spindle pole organization in Xenopus egg extract
    Shusheng Wang
    Department of Embryology, Carnegie Institution for Science and Howard Hughes Medical Institute, Baltimore, Maryland 21218, USA
    J Biol Chem 286:587-93. 2011
    ..Together, our findings open up new avenues to further decipher the mechanism of dynein regulation by Nudel and Lis1...
  36. ncbi CPEB is a specificity factor that mediates cytoplasmic polyadenylation during Xenopus oocyte maturation
    L E Hake
    Worcester Foundation for Experimental Biology Shrewsbury, Massachusetts 01545
    Cell 79:617-27. 1994
    ..Partial restoration of polyadenylation in depleted extracts is achieved by the addition of CPEB, thus demonstrating that this protein is required for cytoplasmic polyadenylation...
  37. ncbi Requirement of prorenin receptor and vacuolar H+-ATPase-mediated acidification for Wnt signaling
    Cristina Maria Cruciat
    Division of Molecular Embryology, DKFZ ZMBH Alliance, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D 69120 Heidelberg, Germany
    Science 327:459-63. 2010
    ..The results reveal an unsuspected role for the prorenin receptor, V-ATPase activity, and acidification during Wnt/beta-catenin signaling...
  38. ncbi Chromatin immunoprecipitation in early Xenopus laevis embryos
    Shelby A Blythe
    Cell and Molecular Biology Graduate Group, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA
    Dev Dyn 238:1422-32. 2009
    ..Developmental Dynamics 238:1422-1432, 2009. (c) 2009 Wiley-Liss, Inc...
  39. ncbi Cardiac septal and valvular dysmorphogenesis in mice heterozygous for mutations in the homeobox gene Nkx2-5
    C Biben
    Victor Chang Cardiac Research Institute, Darlinghurst, Australia
    Circ Res 87:888-95. 2000
    ..Our data demonstrate that the complex effects of Nkx2-5 haploinsufficiency in mice are weaker but convergent with those in humans. As in the mouse, the phenotype of human NKX2-5 mutations may be modulated by interacting alleles...
  40. ncbi Distinct enhancer elements control Hex expression during gastrulation and early organogenesis
    T A Rodriguez
    Division of Mammalian Development, National Institute for Medical Research, The Ridgeway, Mill Hill, London, NW7 1AA, United Kingdom
    Dev Biol 234:304-16. 2001
    ..This analysis also reveals that the initiation of Hex expression in the anterior visceral endoderm and axial mesendoderm requires common elements, but maintenance of expression is regulated independently in these tissues...
  41. ncbi Human replication proteins hCdc21, hCdc46 and P1Mcm3 bind chromatin uniformly before S-phase and are displaced locally during DNA replication
    T Krude
    Wellcome CRC Institute, Cambridge, UK
    J Cell Sci 109:309-18. 1996
    ....
  42. ncbi Tome-1, a trigger of mitotic entry, is degraded during G1 via the APC
    Nagi G Ayad
    Department of Cell Biology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Cell 113:101-13. 2003
    ..Degradation of Tome-1 during G1 allows for wee 1 accumulation during interphase, thereby providing a critical link between the APC and SCF pathways in regulation of cdk1/cyclin B activity and thus mitotic entry and exit...
  43. ncbi Regulation of Msx genes by a Bmp gradient is essential for neural crest specification
    Celeste Tribulo
    Millennium Nucleus in Developmental Biology, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile
    Development 130:6441-52. 2003
    ..We propose a model where a gradient of Bmp activity specifies the expression of Msx genes in the neural folds, and that this expression is essential for the early specification of the neural crest...
  44. ncbi LDL-receptor-related protein 6 is a receptor for Dickkopf proteins
    B Mao
    Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Heidelberg, Germany
    Nature 411:321-5. 2001
    ..Thus, DKKs inhibit Wnt co-receptor function, exemplifying the modulation of LRP signalling by antagonists...
  45. ncbi Translational control by cytoplasmic polyadenylation in Xenopus oocytes
    Helois E Radford
    School of Pharmacy, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, UK
    Biochim Biophys Acta 1779:217-29. 2008
    ..Finally we discuss some of the remaining questions regarding the mechanisms of translational regulation by cytoplasmic polyadenylation and give our view on where our knowledge is likely to be expanded in the near future...
  46. ncbi Greatwall maintains mitosis through regulation of PP2A
    Suzanne Vigneron
    Centre de Recherche de Biochimie Macromoleculaire, CNRS UMR 5237, IFR 122, Labellisée Ligue Nationale Contre le Cancer, Universités Montpellier 2 et 1, Montpellier, France
    EMBO J 28:2786-93. 2009
    ..These results suggest that mitotic entry and maintenance is not only mediated by the activation of cyclin B-Cdc2 but also by the regulation of PP2A by GW...
  47. ncbi The HNF1beta transcription factor has several domains involved in nephrogenesis and partially rescues Pax8/lim1-induced kidney malformations
    Guizhi Wu
    Institut fur Zellbiologie, Universitatsklinikum Essen, Germany
    Eur J Biochem 271:3715-28. 2004
    ..We conclude that HNF1beta has some distinct morphogenetic properties during nephrogenesis...
  48. ncbi The planar polarity gene strabismus regulates convergent extension movements in Xenopus
    Rachel S Darken
    Department of Cell Biology, Weill Medical College, New York, NY 10021, USA
    EMBO J 21:976-85. 2002
    ..Thus our results strongly support the emerging hypothesis that a vertebrate analog of the planar polarity pathway governs convergent extension movements...
  49. ncbi Identification of neurogenin, a vertebrate neuronal determination gene
    Q Ma
    Division of Biology, Howard Hughes Medical Institute, California Institute of Technology, Pasadena 91125, USA
    Cell 87:43-52. 1996
    ..The properties of X-NGNR-1 are thus analogous to those of the Drosophila proneural genes, suggesting that it functions as a vertebrate neuronal determination factor...
  50. ncbi Diversity in the origins of sex chromosomes in anurans inferred from comparative mapping of sexual differentiation genes for three species of the Raninae and Xenopodinae
    Yoshinobu Uno
    Laboratory of Animal Cytogenetics, Biosystems Science Course, Graduate School of Life Science, Hokkaido University, Sapporo, 060 0810, Japan
    Chromosome Res 16:999-1011. 2008
    ..laevis and R. rugosa. Cyp17, Cyp19, Dmrt1, Sox9, and WT1 were localized to autosomes in X. laevis and R. rugosa, suggesting that these five genes probably are not candidates for the sex-determining genes in the two anuran species...
  51. ncbi Differential requirements of BMP and Wnt signalling during gastrulation and neurulation define two steps in neural crest induction
    Ben Steventon
    Department of Cell and Developmental Biology, University College London, London, UK
    Development 136:771-9. 2009
    ..Finally, our results emphasise the importance of considering the order of exposure to signals during an inductive event...
  52. ncbi ANR5, an FGF target gene product, regulates gastrulation in Xenopus
    Hyeyoung A Chung
    Division of Morphogenesis, National Institute for Basic Biology, 38 Nishigonaka, Myodaiji, Okazaki, 444 8585, Japan
    Curr Biol 17:932-9. 2007
    ..Our findings reveal for the first time that xANR5 acts through Rho to regulate gastrulation and is an important cytoplasmic partner of PAPC, whose cytoplasmic partner was previously unknown...
  53. ncbi spib is required for primitive myeloid development in Xenopus
    Ricardo M B Costa
    The Healing Foundation Centre, Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom
    Blood 112:2287-96. 2008
    ..Thus, we conclude that spib sits at the top of the known genetic hierarchy that leads to the specification of primitive myeloid cells in amphibians...
  54. ncbi A crucial role for hnRNP K in axon development in Xenopus laevis
    Yuanyuan Liu
    The Department of Biological Sciences and the Center for Neuroscience Research, University at Albany, State University of New York, Albany, NY 12222, USA
    Development 135:3125-35. 2008
    ..Our study supports the idea that translation of at least a subset of RNAs involved in axon development is controlled by post-transcriptional regulatory modules that have hnRNP K as an essential element...
  55. ncbi HURP is part of a Ran-dependent complex involved in spindle formation
    Maria D Koffa
    EMBL, Heidelberg, Germany
    Curr Biol 16:743-54. 2006
    ..CONCLUSIONS: HURP is a new and essential component of the mitotic apparatus. HURP acts as part of a multicomponent complex that affects the growth or stability of spindle MTs and is required for spindle MT organization...
  56. ncbi TopBP1 activates the ATR-ATRIP complex
    Akiko Kumagai
    Division of Biology 216 76, California Institute of Technology, Pasadena, CA 91125, USA
    Cell 124:943-55. 2006
    ..These studies establish that activation of ATR by TopBP1 is a crucial step in the initiation of ATR-dependent signaling processes...
  57. ncbi Neural crest induction in Xenopus: evidence for a two-signal model
    C LaBonne
    Division of Biology, Beckman Institute 139 74, California Institute of Technology, Pasadena, CA 91125, USA
    Development 125:2403-14. 1998
    ..Slug-expressing ectoderm will generate neural crest in the presence of Wnt or FGF-like signals, however, bypassing the need for BMP inhibition in this process. A two-step model for neural crest induction is proposed...
  58. ncbi Robust stability of the embryonic axial pattern requires a secreted scaffold for chordin degradation
    Hidehiko Inomata
    Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, Kobe 650 0047, Japan
    Cell 134:854-65. 2008
    ..These results indicate that stable axial formation depends on two compensatory regulatory pathways involving ONT1/B1TP and dorsally expressed BMPs...
  59. ncbi Roles of replication fork-interacting and Chk1-activating domains from Claspin in a DNA replication checkpoint response
    Joon Lee
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Mol Biol Cell 16:5269-82. 2005
    ..Another implication of this work is that stable binding of Claspin to chromatin may play a role in other functions besides the activation of Chk1...
  60. ncbi Cardiac expression of the ventricle-specific homeobox gene Irx4 is modulated by Nkx2-5 and dHand
    B G Bruneau
    Department of Genetics, Harvard Medical School, Boston, Massachusetts, 02115, USA
    Dev Biol 217:266-77. 2000
    ..These data indicate that the homeobox gene Irx4 is likely to be an important mediator of ventricular differentiation during cardiac development, which is downstream of Nkx2-5 and dHand...
  61. ncbi Phosphorylation of the cyclin b1 cytoplasmic retention sequence by mitogen-activated protein kinase and Plx
    Susan Walsh
    Duke University Medical Center, Durham, NC 27710, USA
    Mol Cancer Res 1:280-9. 2003
    ..Collectively, these data demonstrate a new role for Erk in mitotic regulation, identify the Ser 101-directed kinase, and provide a picture of cyclin B1/Cdc2 regulation by the combinatorial action of distinct kinases...
  62. ncbi PCNS: a novel protocadherin required for cranial neural crest migration and somite morphogenesis in Xenopus
    Janaki Rangarajan
    Laboratory of Molecular Genetics, NICHD, NIH, Bethesda, MD 20892, USA
    Dev Biol 295:206-18. 2006
    ..Somites and axial muscles also failed to undergo normal morphogenesis in these embryos. Thus, PCNS has essential functions in these two important developmental processes in Xenopus...
  63. ncbi Complementary expression of AP-2 and AP-2rep in ectodermal derivatives of Xenopus embryos
    Masanori Gotoh
    Graduate School of Science and Technology, Niigata University, 8050 Ikarashi-2, Niigata 950-2181, Japan
    Dev Genes Evol 213:363-7. 2003
    ....
  64. ncbi Sox10 is required for the early development of the prospective neural crest in Xenopus embryos
    Stella M Honoré
    Millennium Nucleus in Developmental Biology, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile
    Dev Biol 260:79-96. 2003
    ..However, the overexpression of Slug was not able to upregulate Sox10 expression. Taken together, these results indicate that Sox10 may lie between Snail and Slug in the genetic cascade that controls neural crest development...
  65. ncbi Myocardin is sufficient and necessary for cardiac gene expression in Xenopus
    Eric M Small
    Department of Cell Biology and Anatomy, University of Arizona Health Sciences Center, 1501 N Campbell Avenue, PO Box 245044, Tucson, AZ 85724, USA
    Development 132:987-97. 2005
    ..We conclude that myocardin is an essential component of the regulatory pathway for myocardial differentiation...
  66. ncbi Molecular cloning and characterization of an adaptor protein Shc isoform from Xenopus laevis oocytes
    Franck Chesnel
    UMR 6061 CNRS lt lt Génétique et développement, IFR 97 Université de Rennes 1 35042 Rennes cedex, France
    Biol Cell 95:311-20. 2003
    ....
  67. ncbi Isolation of the B3 transcription factor of the Xenopus TFIIIA gene
    David Griffin
    Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Gene 313:179-88. 2003
    ..Our results suggest that B3.65 is a key regulator of both RNA- and DNA metabolism...
  68. ncbi The beta-catenin/VegT-regulated early zygotic gene Xnr5 is a direct target of SOX3 regulation
    Chi Zhang
    Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80309 0347, USA
    Development 130:5609-24. 2003
    ..These observations indicate that maternal XSOX3 acts in a novel manner to restrict Xnr5 expression to the vegetal hemisphere...
  69. ncbi The Xenopus homeobox gene twin mediates Wnt induction of goosecoid in establishment of Spemann's organizer
    M N Laurent
    Department of Developmental and Cell Biology, and the Developmental Biology Center, University of California, Irvine 92697 2300, USA
    Development 124:4905-16. 1997
    ....
  70. ncbi Sox17 and beta-catenin cooperate to regulate the transcription of endodermal genes
    Debora Sinner
    Cincinnati Children s Hospital Medical Center, Division of Developmental Biology and The Department of Pediatrics, University of Cincinnati College of Medicine, 3333 Burnet Avenue, Cincinnati, OH 45229 3039, USA
    Development 131:3069-80. 2004
    ..These data suggest that in a mechanism analogous to Tcf/Lef interacting with beta-catenin, Sox17 and beta-catenin interact to transcribe endodermal target genes...
  71. ncbi A novel Cripto-related protein reveals an essential role for EGF-CFCs in Nodal signalling in Xenopus embryos
    Karel Dorey
    Laboratory of Developmental Signalling, Cancer Research UK London Research Institute, 44 Lincoln s Inn Fields, WC2A 3PX London, UK
    Dev Biol 292:303-16. 2006
    ..Together our results indicate that the XCRs are important for modulation of the timing and intensity of Nodal signalling in Xenopus embryos...
  72. ncbi Comparative genomic and expression analysis of the conserved NTPDase gene family in Xenopus
    Karine Masse
    Department of Biological Sciences, Warwick University, Coventry, CV47AL, UK
    Genomics 87:366-81. 2006
    ..During development, all NTPDase genes, except for NTPDase8, are expressed and display a distinct specific expression pattern, suggesting potentially different functions of these proteins during embryogenesis of X. laevis...
  73. ncbi Multiple noggins in vertebrate genome: cloning and expression of noggin2 and noggin4 in Xenopus laevis
    Fedor M Eroshkin
    Shemyakin Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16 10 Miklukho Maklaya, Moscow 117997, Russia
    Gene Expr Patterns 6:180-6. 2006
    ..The data presented here demonstrate that the vertebrate phylum contains at least three distinct noggin genes...
  74. ncbi Positive and negative regulation of the transforming growth factor beta/activin target gene goosecoid by the TFII-I family of transcription factors
    Manching Ku
    Molecular Cardiology Research Institute, Tufts New England Medical Center, Boston, MA 02111, USA
    Mol Cell Biol 25:7144-57. 2005
    ..These results suggest a model in which TFII-I family proteins have opposing effects in the regulation of the Gsc gene in response to a TGFbeta/activin signal...
  75. ncbi Functional specificity of the Xenopus T-domain protein Brachyury is conferred by its ability to interact with Smad1
    Nigel J Messenger
    Wellcome Trust Cancer Research UK Gurdon Institute, Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, United Kingdom
    Dev Cell 8:599-610. 2005
    ..These findings suggest a mechanism by which individual T-domain proteins may interact with different partners to elicit a specific response...
  76. ncbi Noncanonical Wnt-4 signaling and EAF2 are required for eye development in Xenopus laevis
    Daniel Maurus
    Department of Biochemistry, University of Ulm, Ulm, Germany
    EMBO J 24:1181-91. 2005
    ..These data add a new layer of complexity to our understanding of eye development and give further evidence for the importance of noncanonical Wnt pathways in organ development...
  77. ncbi SOX7 is an immediate-early target of VegT and regulates Nodal-related gene expression in Xenopus
    Chi Zhang
    Molecular, Cellular and Developmental Biology, University of Colorado, Porter Biosci Building, Boulder, CO 80309 0347, USA
    Dev Biol 278:526-41. 2005
    ..2004. Dev. Biol. 273, 23) and their common binding sites in the Xnr5 promoter, we propose a model in which competitive interactions between these two proteins are involved in refining the domain of endodermal differentiation...
  78. ncbi One of the duplicated matrix metalloproteinase-9 genes is expressed in regressing tail during anuran metamorphosis
    Kenta Fujimoto
    Division of Embryology and Genetics, Institute for Amphibian Biology, Graduate School of Science, Hiroshima University, Higashihiroshima 739 8526, Japan
    Dev Growth Differ 48:223-41. 2006
    ..laevis and X. tropicalis to be expressed in degenerating and remodeling organs as a response to thyroid hormone during metamorphosis...
  79. ncbi bHLH-dependent and -independent modes of Ath5 gene regulation during retinal development
    David A Hutcheson
    Department of Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City, UT 84132, USA
    Development 132:829-39. 2005
    ..Thus, despite extensive conservation in the proximal region, the importance of these elements may be species dependent...
  80. ncbi Isolation and comparative expression analysis of the Myc-regulatory proteins Mad1, Mad3, and Mnt during Xenopus development
    Kathrin Juergens
    Department of Developmental Biochemistry, University of Goettingen, Goettingen, Germany
    Dev Dyn 233:1554-9. 2005
    ..This study demonstrates the Mads are expressed in specific and mostly nonoverlapping patterns, suggesting distinct roles during embryogenesis...
  81. ncbi Stage-specific effects of retinoic acid on gene expression during forebrain development
    Gerald W Eagleson
    Department of Biology, Loras College Dubuque, IA 52004 0178, USA
    Brain Res Bull 75:281-8. 2008
    ..Xsix3 seems to be very important for the initial parcellation of telencephalon, retinal and diencephalon areas...
  82. ncbi Xenopus X-box binding protein 1, a leucine zipper transcription factor, is involved in the BMP signaling pathway
    Hui Zhao
    Department of Zoophysiology, University of Essen, Universitatsstrasse 5, D 45117 Essen, Germany
    Dev Biol 257:278-91. 2003
    ..Taken together, the results suggest a role for XXBP-1 in the control of neural differentiation, possibly as an activator...
  83. ncbi A vegetally localized T-box transcription factor in Xenopus eggs specifies mesoderm and endoderm and is essential for embryonic mesoderm formation
    M E Horb
    Department of Biochemistry and Cell Biology, Institute for Cell and Developmental Biology, State University of New York, Stony Brook 11794 5215, USA
    Development 124:1689-98. 1997
    ..This treatment inhibited mesoderm formation and severely disrupted normal development, thereby establishing that Brat plays a critical role in embryonic mesoderm formation and body patterning...
  84. ncbi The Xenopus homologue of the Drosophila gene tailless has a function in early eye development
    T Hollemann
    Institut fur Biochemie und Molekulare Zellbiologie, Humboldtallee 23, D 37073 Gottingen, Germany
    Development 125:2425-32. 1998
    ..These findings suggest that Xtll serves an important regulatory function in the earliest phases of vertebrate eye development...
  85. ncbi Bix1, a direct target of Xenopus T-box genes, causes formation of ventral mesoderm and endoderm
    M Tada
    Division of Developmental Biology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Development 125:3997-4006. 1998
    ..These results suggest that Bix1 acts downstream of both VegT and Xbra to induce formation of mesoderm and endoderm...
  86. ncbi cDNA cloning and expression during development of Drosophila melanogaster MCM3, MCM6 and MCM7
    K Ohno
    Laboratory of Cell Biology, Aichi Cancer Center Research Institute, Chikusa ku, Nagoya 464 8681, Japan
    Gene 217:177-85. 1998
    ....
  87. ncbi Spatiotemporal, allelic, and enforced expression of Ximpact, the Xenopus homolog of mouse imprinted gene impact
    Y Yamada
    Human Genome Center, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Tokyo, Minato ku, 108 8639, Japan
    Biochem Biophys Res Commun 256:162-9. 1999
    ..Overexpression of Ximpact by RNA microinjection resulted in a higher than normal rate of gastrulation defects, suggesting the need for tight control of its dosage in early development...
  88. ncbi Spatially and temporally-restricted expression of two T-box genes during zebrafish embryogenesis
    S Yonei-Tamura
    The Salk Institute for Biological Studies, Gene Expression Laboratory, 10010 N Torrey Pines Road, La Jolla, CA 92037 1099, USA
    Mech Dev 80:219-21. 1999
    ..Zf-tbr1 and zf-tbx3 share high amino acid identity with human, murine, chick and Xenopus orthologs and are expressed in specific regions during zebrafish development...
  89. ncbi XCtBP is a XTcf-3 co-repressor with roles throughout Xenopus development
    M Brannon
    Department of Biochemistry, Howard Hughes Medical Institute, Center for Developmental Biology, University of Washington School of Medicine, Seattle, Washington 98195 7350, USA
    Development 126:3159-70. 1999
    ..Our data support a role for XCtBP as a co-repressor throughout Xenopus development and indicate that XCtBP/G4A will be a useful tool in determining how XCtBP functions in various developmental processes...
  90. ncbi A role for the homeobox gene Xvex-1 as part of the BMP-4 ventral signaling pathway
    E Shapira
    Department of Cellular Biochemistry, Faculty of Medicine, Hebrew University, P O Box 12272, Jerusalem 91120, Israel
    Mech Dev 86:99-111. 1999
    ..These results place Xvex-1 downstream of BMP-4 during gastrulation and suggest that it represents a novel homeobox family in Xenopus which is part of the ventral signaling pathway...
  91. ncbi Genomic structure and embryonic expression of the Xenopus winged helix factors XFD-13/13'
    M Koster
    Abteilung Biochemie, Universitat Ulm, Germany
    Mech Dev 88:89-93. 1999
    ..Since they are subsequently found to accumulate in vessel like structures, we suggest that these cells represent hematopoietic/endothelial progenitor cells...
  92. ncbi Cerberus-like is a secreted factor with neutralizing activity expressed in the anterior primitive endoderm of the mouse gastrula
    J A Belo
    Department of Biological Chemistry, Howard Hughes Medical Institute, University of California, Los Angeles 90095 1662, USA
    Mech Dev 68:45-57. 1997
    ..A model of how head and trunk development might be regulated is discussed. Given its neuralizing activity, the secreted protein Cer-l is a candidate for mediating inductive activities of anterior visceral endoderm...
  93. ncbi An interferon regulatory factor-related gene (xIRF-6) is expressed in the posterior mesoderm during the early development of Xenopus laevis
    S Hatada
    Department of Biological Science, Graduate School of Science, University of Tokyo, Japan
    Gene 203:183-8. 1997
    ..The temporally and spatially limited expression of the xIRF-6 gene product may contribute to the transcriptional regulation of specific genes which are necessary for the development of the posterior somites...
  94. ncbi Sequential expression of multiple POU proteins during amphibian early development
    C S Hinkley
    Department of Biochemistry and Molecular Biology, University of Texas M D Anderson Cancer Center, Houston 77030
    Mol Cell Biol 12:638-49. 1992
    ..By analogy with other members of the POU-domain gene family, the products of these genes may play critical roles in the determination of cell fate and the regulation of cell proliferation...
  95. ncbi Expression of murine Lhx5 suggests a role in specifying the forebrain
    H Z Sheng
    Laboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Dev Dyn 208:266-77. 1997
    ..5 of gestation. The early, persistent, and dynamic expression of Lhx5 suggests a regulatory function in forebrain formation...
  96. ncbi Expression cloning of Siamois, a Xenopus homeobox gene expressed in dorsal-vegetal cells of blastulae and able to induce a complete secondary axis
    P Lemaire
    Wellcome Trust Cancer Research Campaign Institute, England
    Cell 81:85-94. 1995
    ..The activation of this gene can be obtained cell autonomously in dispersed embryo cells. These results indicate that Siamois may play an important role in the formation of the Nieuwkoop center...
  97. ncbi Dorsal-ventral patterning during neural induction in Xenopus: assessment of spinal cord regionalization with xHB9, a marker for the motor neuron region
    M S Saha
    Department of Biology, The College of William and Mary, Williamsburg, Virginia 23187, USA
    Dev Biol 187:209-23. 1997
    ..Our data suggest that, in addition to floor plate signaling, continued interactions with the underlying mesoderm through neural tube stages are essential for proper spatial patterning of the motor neuron region...
  98. ncbi Cloning and developmental expression of Xenopus cDNAs encoding the Enhancer of split groucho and related proteins
    B K Choudhury
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Gene 195:41-8. 1997
    ..8 kb and 2.2 kb, respectively, were ubiquitously expressed in the developing embryos. The overall structural relationships of ESG and AES proteins among human, mouse, rat, Xenopus and Drosophila were analysed...
  99. ncbi Xbap, a vertebrate gene related to bagpipe, is expressed in developing craniofacial structures and in anterior gut muscle
    C S Newman
    Center for Developmental Biology, Department of Zoology, University of Texas at Austin, 78712, USA
    Dev Biol 181:223-33. 1997
    ..This vertebrate bagpipe sequence therefore is expressed in both mesodermally and neural crest-derived tissues...
  100. ncbi Isolation and expression study of a maternally expressed novel Xenopus gene Xem1 encoding a putative evolutionarily conserved membrane protein
    N Adati
    Department of Biological Sciences, Graduate School of Science, University of Tokyo, Japan
    Biochem Biophys Res Commun 238:899-904. 1997
    ....
  101. ncbi Characterization and early embryonic expression of a neural specific transcription factor xSOX3 in Xenopus laevis
    R Penzel
    Universität GH Essen, Department of Zoophysiology, Germany
    Int J Dev Biol 41:667-77. 1997
    ..The gene can also be activated in dissociated gastrula ectoderm without mesodermal or neural inducers. That means that the gene can be expressed in ectodermal cells in a cell autonomous manner...

Research Grants108 found, 100 shown here

  1. MARCKS Phosphorylation in Growth Cone Signaling
    JESSE GATLIN; Fiscal Year: 2004
    ..Functional consequences of aberrant PKC signaling will be determined by observing the turning response of growth cones from DRG neurons expressing a dominant-negative, phosphorylation-deficient MARCKS mutant. ..
  2. Computation tools for analyzing gene expression data
    Naama Barkai; Fiscal Year: 2004
    ..Kinetic microarray experiments that highlight specific features of the transcriptional network will be designed, performed and analyzed. Methods for extracting regulatory relationships from these experiments will be developed. ..
  3. THE ROLE OF MAP KINASE IN THE CELL CYCLE
    JAMES FERRELL; Fiscal Year: 2005
    ..4. To examine the systems-level properties of a p42 MAPK cascade reconstituted with recombinant Xenopus proteins.Our overarching aim is to better understand the cell biology and biochemistry of oocyte maturation and early ..
  4. Intrinsic Spindle Mechanisms to Insure Bipolarity
    JESSE GATLIN; Fiscal Year: 2007
    ....
  5. MOLECULAR GENETICS OF EARLY NEUROGENESIS
    Ethan Bier; Fiscal Year: 2007
    ..Therefore, our comparative studies in cell fate specification are relevant to using Drosophila as a model for drug addiction. These studies may provide the framework for our future studies relevant to human disease and drug abuse. ..
  6. TRAINING IN MOLECULAR AND CELLULAR BIOLOGY
    David Kimelman; Fiscal Year: 2007
    ..The trainees have a strong record of success as shown by their publications in graduate school and the career choices they make after they leave graduate school. ..
  7. MOLECULAR REGULATION OF EARLY XENOPUS DEVELOPMENT
    David Kimelman; Fiscal Year: 2003
    ..Since the Wnt pathway is implicated in normal vertebrate development and oncogenesis, these studies will be important in understanding the molecular basis of birth defects and cancer. ..
  8. Combinatorial signaling in zebrafish development
    David Kimelman; Fiscal Year: 2005
    ..abstract_text> ..
  9. EUKARYOTIC HYALURONAN SYNTHASES
    PAUL DEANGELIS; Fiscal Year: 2001
    ..The essential residues identified in aim 3 will also be confirmed by mutagenesis studies. The enzymological and structural information may allow the generation of a working model describing the catalytic mechanism of these enzymes. ..
  10. Regulation of mesodermal progenitors in transgenic zebrafish
    David Kimelman; Fiscal Year: 2009
    ....
  11. Regulation of mesodermal progenitors in transgenic zebrafish
    David Kimelman; Fiscal Year: 2010
    ....
  12. Wnt Pathway Regulation in Embryos and Cells
    David Kimelman; Fiscal Year: 2009
    ..Since the Wnt pathway plays key roles in vertebrate development and oncogenesis, these studies will be important in advancing knowledge of the molecular basis of birth defects and cancer. ..
  13. MOLECULAR REGULATION OF EARLY XENOPUS DEVELOPMENT
    David Kimelman; Fiscal Year: 1993
    ..Since bFGF s not only involved in development regulation but can also convert common issues in different medical fields...
  14. Cytoskeleton polymerization dynamics in the cell cycle
    Timothy Mitchison; Fiscal Year: 2007
    ..Understanding midbody assembles will reveal principles for generating asymmetric assemblies in the cytoplasm, and will tell us how the cleavage furrow is positioned. ..
  15. ELECTRON MICROSCOPE
    Timothy Mitchison; Fiscal Year: 2004
    ..The automated controls and digital image acquisition will also be optimal for introducing new users to electron microscopy and for minimizing maintenance requirements that might arise from operator inexperience. ..
  16. ACTIN DYNAMICS AND CELL LOCOMOTION
    Timothy Mitchison; Fiscal Year: 2004
    ..For perturbation experiments we will use protein tools, and also cell permeable chemicals coming out of a screening effort at the Harvard Institute of Chemistry and Cell Biology (ICCB). ..
  17. Cytoskeleton polymerization dynamics in the cell cycle
    Timothy J Mitchison; Fiscal Year: 2010
    ..Understanding midbody assembles will reveal principles for generating asymmetric assemblies in the cytoplasm, and will tell us how the cleavage furrow is positioned. ..
  18. MICROTUBULE DYNAMICS AND MITOTIC MECHANISM
    Timothy J Mitchison; Fiscal Year: 2010
    ..These discoveries will have immediate relevance to the development of novel cancer drugs. ..
  19. Biochemistry and Cytology of Cytokinesis
    Timothy Mitchison; Fiscal Year: 2006
    ..We will probe how the microtubules associated with cytokinesis are organized in the cell and what mechanisms they use to target anillin and myosin II to the center of the cell to build a furrow there. ..
  20. MICROTUBULE DYNAMICS AND MITOTIC MECHANISM
    Timothy Mitchison; Fiscal Year: 2007
    ..These discoveries will have immediate relevance to the development of novel cancer drugs. ..
  21. ACTIN DYNAMICS AND CELL LOCOMOTION
    Timothy Mitchison; Fiscal Year: 2000
    ..They then intend to use what they learn in Listeria and apply it to understanding the movement of whole cells. One focus will be the mechanism by which the filopodia of neuronal growth cones extend and retract. ..
  22. GENETIC AND BIOCHEMICAL ANALYSIS OF CYTOKINESIS
    Timothy Mitchison; Fiscal Year: 2000
    ....
  23. Transcriptional regulatory networks controlling Xenopus pancreas development
    MARKO HORB; Fiscal Year: 2009
    ....
  24. 2006 Biophysical Discussions - Molecuar Motors: Point Counterpoint
    Steven Rosenfeld; Fiscal Year: 2006
    ....
  25. Transcriptional regulatory networks controlling Xenopus pancreas development
    Marko E Horb; Fiscal Year: 2010
    ....
  26. Mechanisms of Processivity in Molecular Motors
    Steven Rosenfeld; Fiscal Year: 2007
    ..Furthermore, if successful, this work will support the argument that understanding how a motor works in vitro as an enzyme can provide valuable insights into how it works in vivo in the cell. ..
  27. Development of High Throughput Screening Assays (RMI)
    Steven Rosenfeld; Fiscal Year: 2004
    ..This work will serve as the basis for a future extramural funding initiative designed to take promising compounds identified by this approach into pre-clinical glioma model systems, and ultimately, into clinical trial. ..
  28. ANALYSIS OF EARLY WING VEIN DEVELOPMENT IN DROSPHILIA
    Ethan Bier; Fiscal Year: 2004
    ..Because mis-regulation of these known pathways causes aggressive forms of cancer, understanding the regulation of these pathways also is of significant relevance to cancer. ..
  29. MOLECULAR GENETICS OF EARLY NEUROGENESIS
    Ethan Bier; Fiscal Year: 2004
    ..2) Determine whether different forms of Sog block selected subsets of BMP ligands. 3) Analyze positive acting forms of Sog and determine their range of action. ..
  30. MOLECULAR GENETICS OF NEUROGENESIS
    Ethan Bier; Fiscal Year: 1993
    ..g modifiers of the scratched eye phenotype of putative scrt alleles). Finally, we will use low stringency hybridization and PCR amplification with scrt zinc finger probes and primers to search for structurally related pan-neural genes...
  31. MOLECULAR GENETICS OF EARLY NEUROGENESIS
    Ethan Bier; Fiscal Year: 2000
    ..III. Identify enhancer element(s) directing sog expression during embryonic and adult development. IV. Determine whether a Sog protein(s) is secreted, diffuses, and binds to Dpp. ..
  32. Genetic Analysis of Bacterial Toxins in Drosophia
    Ethan Bier; Fiscal Year: 2005
    ..DN alleles 2-2: Characterize molecular lesion in DN-toxin NOVA alleles 2-3: Confirm that DN-toxin alleles interfere with wild-type toxin gene function 2-4: Determine whether DN-toxin alleles function in mammalian cells ..