xenopus proteins

Summary

Summary: Proteins obtained from various species of Xenopus. Included here are proteins from the African clawed frog (XENOPUS LAEVIS). Many of these proteins have been the subject of scientific investigations in the area of MORPHOGENESIS and development.

Top Publications

  1. pmc The genome of the Western clawed frog Xenopus tropicalis
    Uffe Hellsten
    Department of Energy Joint Genome Institute, Walnut Creek, CA 94598, USA
    Science 328:633-6. 2010
  2. ncbi XTcf-3 transcription factor mediates beta-catenin-induced axis formation in Xenopus embryos
    M Molenaar
    Department of Immunology University Hospital, Utrecht The Netherlands
    Cell 86:391-9. 1996
  3. pmc Functional uncoupling of MCM helicase and DNA polymerase activities activates the ATR-dependent checkpoint
    Tony S Byun
    Department of Molecular Pharmacology, Stanford University, Stanford, CA 94305, USA
    Genes Dev 19:1040-52. 2005
  4. ncbi The bipolar mitotic kinesin Eg5 moves on both microtubules that it crosslinks
    Lukas C Kapitein
    Department of Physics and Astronomy and Laser Centre, Vrije Universiteit, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands
    Nature 435:114-8. 2005
  5. ncbi Geminin, an inhibitor of DNA replication, is degraded during mitosis
    T J McGarry
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cell 93:1043-53. 1998
  6. ncbi The axis-inducing activity, stability, and subcellular distribution of beta-catenin is regulated in Xenopus embryos by glycogen synthase kinase 3
    C Yost
    Department of Biochemistry, University of Washington School of Medicine, Seattle, 98195 7370, USA
    Genes Dev 10:1443-54. 1996
  7. ncbi TopBP1 activates the ATR-ATRIP complex
    Akiko Kumagai
    Division of Biology 216 76, California Institute of Technology, Pasadena, CA 91125, USA
    Cell 124:943-55. 2006
  8. ncbi Aurora-A and an interacting activator, the LIM protein Ajuba, are required for mitotic commitment in human cells
    Toru Hirota
    Department of Tumor Genetics and Biology, Graduate School of Medical Sciences, Kumamoto University, Japan
    Cell 114:585-98. 2003
  9. ncbi Inhibition of eukaryotic DNA replication by geminin binding to Cdt1
    J A Wohlschlegel
    Department of Pathology, Brigham and Women s Hospital, Harvard Medical School, 75 Francis Street, Boston, MA 02115, USA
    Science 290:2309-12. 2000
  10. pmc The M phase kinase Greatwall (Gwl) promotes inactivation of PP2A/B55delta, a phosphatase directed against CDK phosphosites
    Priscila V Castilho
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
    Mol Biol Cell 20:4777-89. 2009

Research Grants

  1. MARCKS Phosphorylation in Growth Cone Signaling
    JESSE GATLIN; Fiscal Year: 2004
  2. Computation tools for analyzing gene expression data
    Naama Barkai; Fiscal Year: 2004
  3. Analysis of Early Wing Vein Development in Drosophila
    Ethan Bier; Fiscal Year: 2007
  4. Wnt Pathway Regulation in Embryos and Cells
    David Kimelman; Fiscal Year: 2009
  5. Combinatorial signaling in zebrafish development
    David Kimelman; Fiscal Year: 2005
  6. EUKARYOTIC HYALURONAN SYNTHASES
    PAUL DEANGELIS; Fiscal Year: 2001
  7. Probing Cell Division with Synthetic Chemistry
    Timothy Mitchison; Fiscal Year: 2008
  8. ACTIN DYNAMICS AND CELL LOCOMOTION
    Timothy Mitchison; Fiscal Year: 2004
  9. Cytoskeleton polymerization dynamics in the cell cycle
    Timothy J Mitchison; Fiscal Year: 2010
  10. ELECTRON MICROSCOPE
    Timothy Mitchison; Fiscal Year: 2004

Detail Information

Publications295 found, 100 shown here

  1. pmc The genome of the Western clawed frog Xenopus tropicalis
    Uffe Hellsten
    Department of Energy Joint Genome Institute, Walnut Creek, CA 94598, USA
    Science 328:633-6. 2010
    ..The genome exhibits substantial shared synteny with human and chicken over major parts of large chromosomes, broken by lineage-specific chromosome fusions and fissions, mainly in the mammalian lineage...
  2. ncbi XTcf-3 transcription factor mediates beta-catenin-induced axis formation in Xenopus embryos
    M Molenaar
    Department of Immunology University Hospital, Utrecht The Netherlands
    Cell 86:391-9. 1996
    ..We propose that signaling by beta-catenin involves complex formation with XTcf-3, followed by nuclear translocation and activation of specific XTcf-3 target genes...
  3. pmc Functional uncoupling of MCM helicase and DNA polymerase activities activates the ATR-dependent checkpoint
    Tony S Byun
    Department of Molecular Pharmacology, Stanford University, Stanford, CA 94305, USA
    Genes Dev 19:1040-52. 2005
    ....
  4. ncbi The bipolar mitotic kinesin Eg5 moves on both microtubules that it crosslinks
    Lukas C Kapitein
    Department of Physics and Astronomy and Laser Centre, Vrije Universiteit, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands
    Nature 435:114-8. 2005
    ..Our results demonstrate how members of the kinesin-5 family are likely to function in mitosis, pushing apart interpolar microtubules as well as recruiting microtubules into bundles that are subsequently polarized by relative sliding...
  5. ncbi Geminin, an inhibitor of DNA replication, is degraded during mitosis
    T J McGarry
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cell 93:1043-53. 1998
    ..We propose that geminin inhibits DNA replication during S, G2, and M phases and that geminin destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle...
  6. ncbi The axis-inducing activity, stability, and subcellular distribution of beta-catenin is regulated in Xenopus embryos by glycogen synthase kinase 3
    C Yost
    Department of Biochemistry, University of Washington School of Medicine, Seattle, 98195 7370, USA
    Genes Dev 10:1443-54. 1996
    ..These studies provide a basis for understanding the interaction between Xgsk-3 and beta-catenin in the establishment of the dorsal-ventral axis in early Xenopus embryos...
  7. ncbi TopBP1 activates the ATR-ATRIP complex
    Akiko Kumagai
    Division of Biology 216 76, California Institute of Technology, Pasadena, CA 91125, USA
    Cell 124:943-55. 2006
    ..These studies establish that activation of ATR by TopBP1 is a crucial step in the initiation of ATR-dependent signaling processes...
  8. ncbi Aurora-A and an interacting activator, the LIM protein Ajuba, are required for mitotic commitment in human cells
    Toru Hirota
    Department of Tumor Genetics and Biology, Graduate School of Medical Sciences, Kumamoto University, Japan
    Cell 114:585-98. 2003
    ..Depletion of Ajuba prevented activation of Aurora-A at centrosomes in late G2 phase and inhibited mitotic entry. Overall, our data suggest that Ajuba is an essential activator of Aurora-A in mitotic commitment...
  9. ncbi Inhibition of eukaryotic DNA replication by geminin binding to Cdt1
    J A Wohlschlegel
    Department of Pathology, Brigham and Women s Hospital, Harvard Medical School, 75 Francis Street, Boston, MA 02115, USA
    Science 290:2309-12. 2000
    ..In the normal cell cycle, Cdt1 is present only in G1 and S, whereas geminin is present in S and G2 phases of the cell cycle. Together, these results suggest that geminin inhibits inappropriate origin firing by targeting Cdt1...
  10. pmc The M phase kinase Greatwall (Gwl) promotes inactivation of PP2A/B55delta, a phosphatase directed against CDK phosphosites
    Priscila V Castilho
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
    Mol Biol Cell 20:4777-89. 2009
    ..These findings support the hypothesis that M phase requires not only high levels of MPF function, but also the suppression, through a Gwl-dependent mechanism, of phosphatase(s) that would otherwise remove MPF-driven phosphorylations...
  11. ncbi Biochemical and cellular effects of roscovitine, a potent and selective inhibitor of the cyclin-dependent kinases cdc2, cdk2 and cdk5
    L Meijer
    CNRS, Station Biologique, Roscoff, France
    Eur J Biochem 243:527-36. 1997
    ..Through its unique selectivity for some cyclin-dependent kinases, roscovitine provides a useful antimitotic reagent for cell cycle studies and may prove interesting to control cells with deregulated cdc2, cdk2 or cdk5 kinase activities...
  12. ncbi Maternal wnt11 activates the canonical wnt signaling pathway required for axis formation in Xenopus embryos
    Qinghua Tao
    Division of Developmental Biology, Cincinnati Children s Research Foundation, 3333 Burnet Avenue, Cincinnati, Ohio 45229, USA
    Cell 120:857-71. 2005
    ..Second, we show that activation requires the glycosyl transferase X.EXT1. Third, we find that the EGF-CFC protein, FRL1, is also essential and interacts with Wnt11 to activate canonical Wnt signaling...
  13. ncbi Myt1: a membrane-associated inhibitory kinase that phosphorylates Cdc2 on both threonine-14 and tyrosine-15
    P R Mueller
    Division of Biology 216 76, Howard Hughes Medical Institute, California Institute of Technology, Pasadena 91125, USA
    Science 270:86-90. 1995
    ..Myt1 activity is highly regulated during the cell cycle, suggesting that this relative of Wee1 plays a role in mitotic control...
  14. ncbi A unified view of the DNA-damage checkpoint
    Justine Melo
    Department of Biochemistry and Biophysics, Mount Zion Cancer Research Center, University of California, San Francisco, CA 94115, USA
    Curr Opin Cell Biol 14:237-45. 2002
    ..This review compares the role of these four classes of checkpoint proteins in humans and model organisms...
  15. ncbi The AAA-ATPase Cdc48/p97 regulates spindle disassembly at the end of mitosis
    Kan Cao
    Department of Embryology, Carnegie Institution of Washington and Howard Hughes Medical Institute, Baltimore, MD 21210, USA
    Cell 115:355-67. 2003
    ..Therefore, Cdc48/p97-Ufd1-Npl4 is an essential chaperone that regulates transformation of the microtubule structure as cells reenter interphase...
  16. ncbi Control of beta-catenin phosphorylation/degradation by a dual-kinase mechanism
    Chunming Liu
    Division of Neuroscience, Children s Hospital, Department of Neurology, Harvard Medical School, Boston, MA 02115, USA
    Cell 108:837-47. 2002
    ..Our study uncovers distinct roles and steps of beta-catenin phosphorylation, identifies CKIalpha as a component in Wnt/beta-catenin signaling, and has implications to pathogenesis/therapeutics of human cancers and diabetes...
  17. pmc Collective chemotaxis requires contact-dependent cell polarity
    Eric Theveneau
    Department of Cell and Developmental Biology, University College London, London WC1E 6BT, UK
    Dev Cell 19:39-53. 2010
    ....
  18. pmc Regulated activity of PP2A-B55 delta is crucial for controlling entry into and exit from mitosis in Xenopus egg extracts
    Satoru Mochida
    Cancer Research UK, London Research Institute, Clare Hall Laboratories, South Mimms, Herts, UK
    EMBO J 28:2777-85. 2009
    ..Increasing the concentration of PP2A-B55 delta in extracts by adding recombinant enzyme inhibited the entry into mitosis. This form of PP2A seems to be a key regulator of entry into and exit from mitosis...
  19. pmc The miR-30 miRNA family regulates Xenopus pronephros development and targets the transcription factor Xlim1/Lhx1
    Raman Agrawal
    Department of Cell Biology and Anatomy, LSU Health Sciences Center, MEB 6A12, 1901 Perdido Street, New Orleans, LA 70112, USA
    Development 136:3927-36. 2009
    ..However, in the absence of miR-30 activity, Xlim1/Lhx1 is maintained at high levels and, therefore, may contribute to the delayed terminal differentiation of the amphibian pronephros...
  20. ncbi Initiation of Wnt signaling: control of Wnt coreceptor Lrp6 phosphorylation/activation via frizzled, dishevelled and axin functions
    Xin Zeng
    The F M Kirby Neurobiology Center, Children s Hospital Boston, Department of Neurology, Harvard Medical School, Boston, MA 02115, USA
    Development 135:367-75. 2008
    ..We discuss the dual roles of the axin-Gsk3 complex and signal amplification by Lrp6-axin interaction during Wnt/beta-catenin signaling...
  21. ncbi Casein kinase I transduces Wnt signals
    J M Peters
    Center for Developmental Biology, Department of Molecular Biology, UT Southwestern Medical Center, Dallas, Texas 75235 9133, USA
    Nature 401:345-50. 1999
    ..In addition, CKI bound to and increased the phosphorylation of dishevelled, a known component of the Wnt pathway. These data indicate that CKI may be a conserved component of the Wnt pathway...
  22. ncbi Hairy2 functions through both DNA-binding and non DNA-binding mechanisms at the neural plate border in Xenopus
    Massimo Nichane
    Laboratoire d Embryologie Moleculaire, Universite Libre de Bruxelles, Institut de Biologie et de Médecine Moléculaires IBMM, Gosselies, Belgium
    Dev Biol 322:368-80. 2008
    ..Together, these results suggest that Hairy2 has a dual mode of action and may function at the neural plate border through both a DNA-binding and a non-DNA-binding Stat3-Delta1 mediated mechanism...
  23. pmc The head inducer Cerberus is a multifunctional antagonist of Nodal, BMP and Wnt signals
    S Piccolo
    Howard Hughes Medical Institute and Department of Biological Chemistry, University of California, Los Angeles 90095 1662, USA
    Nature 397:707-10. 1999
    ..In order for the head territory to form, we propose that signals involved in trunk development, such as those involving BMP, Wnt and Nodal proteins, must be inhibited in rostral regions...
  24. ncbi The forkhead protein Foxj1 specifies node-like cilia in Xenopus and zebrafish embryos
    Jennifer L Stubbs
    The Salk Institute for Biological Studies, San Diego, CA 92186, USA
    Nat Genet 40:1454-60. 2008
    ..These results indicate that Foxj1 is a critical determinant in the specification of cilia used in left-right patterning...
  25. pmc Dorsal-ventral patterning and neural induction in Xenopus embryos
    Edward M De Robertis
    Howard Hughes Medical Institute, Department of Biological Chemistry, University of California Los Angeles, Los Angeles, CA 90095 1662, USA
    Annu Rev Cell Dev Biol 20:285-308. 2004
    ..Many of the molecular players that regulate D-V patterning via regulation of BMP signaling have been conserved between Drosophila and the vertebrates...
  26. ncbi A heterodimeric coiled-coil protein required for mitotic chromosome condensation in vitro
    T Hirano
    Department of Pharmacology, University of California, San Francisco 94143 0450
    Cell 79:449-58. 1994
    ..These motifs are highly conserved in a protein family, members of which have been identified recently in both prokaryotes and eukaryotes...
  27. pmc MCM2-7 form double hexamers at licensed origins in Xenopus egg extract
    Agnieszka Gambus
    Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, United Kingdom
    J Biol Chem 286:11855-64. 2011
    ..The CMG proteins also associate less strongly with other replication proteins, consistent with the idea that a single CMG complex forms the core of the replisome...
  28. ncbi Wnt-11 activation of a non-canonical Wnt signalling pathway is required for cardiogenesis
    Petra Pandur
    1 Abt Entwicklungsbiochemie, Junior Group SFB 271, Universitat Gottingen, Humboldtallee 23, D 37073 Gottingen, Germany 2 Abt Biochemie, Universitat Ulm, Albert Einstein Allee 11, D 89081 Ulm, Germany
    Nature 418:636-41. 2002
    ..Our results indicate that the cardiac developmental program requires non-canonical Wnt signal transduction...
  29. ncbi A morphogen gradient of Wnt/beta-catenin signalling regulates anteroposterior neural patterning in Xenopus
    C Kiecker
    Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
    Development 128:4189-201. 2001
    ..Our results indicate that an activity gradient of Wnt/beta-catenin signalling acts as transforming morphogen to pattern the Xenopus central nervous system...
  30. pmc TGF-beta signaling is required for multiple processes during Xenopus tail regeneration
    Diana M Ho
    Department of Developmental Biology, Harvard School of Dental Medicine, Boston, MA 02115, USA
    Dev Biol 315:203-16. 2008
    ....
  31. ncbi A minimal midzone protein module controls formation and length of antiparallel microtubule overlaps
    Peter Bieling
    Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Cell 142:420-32. 2010
    ..Our results mechanistically explain how the two conserved, essential midzone proteins PRC1 and Xklp1 cooperate to constitute a minimal protein module capable of dynamically organizing the core structure of the central anaphase spindle...
  32. pmc Frzb-1 is a secreted antagonist of Wnt signaling expressed in the Spemann organizer
    L Leyns
    Department of Biological Chemistry, University of California, Los Angeles 90095 1662, USA
    Cell 88:747-56. 1997
    ..Cultured cells transfected with a membrane-tethered form of Wnt-1 bind epitope-tagged Frzb-1 in the 10(-10) M range. The results strengthen the view that the Spemann organizer is a source of secreted inhibitory factors...
  33. pmc Human TPX2 is required for targeting Aurora-A kinase to the spindle
    Thomas A Kufer
    Department of Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18a, D 82152 Martinsried, Germany
    J Cell Biol 158:617-23. 2002
    ..We propose that human TPX2 is required for targeting Aurora-A kinase to the spindle apparatus. In turn, Aurora-A might regulate the function of TPX2 during spindle assembly...
  34. pmc Repression of origin assembly in metaphase depends on inhibition of RLF-B/Cdt1 by geminin
    S Tada
    CRC Chromosome Replication Research Group, Wellcome Trust Biocentre, Dow Street University of Dundee, Dundee DD1 5EH, UK
    Nat Cell Biol 3:107-13. 2001
    ..These experiments suggest that geminin-mediated inhibition of RLF-B/Cdt1 is essential for repressing origin assembly late in the cell cycle of higher eukaryotes...
  35. pmc Localization of the kinesin-like protein Xklp2 to spindle poles requires a leucine zipper, a microtubule-associated protein, and dynein
    T Wittmann
    European Molecular Biology Laboratory, Cell Biology and Cell Biophysics Programs, D 69117 Heidelberg, Germany
    J Cell Biol 143:673-85. 1998
    ..We propose two molecular mechanisms that could account for the localization of Xklp2 to microtubule minus ends...
  36. ncbi The left-right axis is regulated by the interplay of Coco, Xnr1 and derrière in Xenopus embryos
    Alin Vonica
    The Laboratory of Vertebrate Embryology, The Rockefeller University, New York, NY 10021, USA
    Dev Biol 303:281-94. 2007
    ..We conclude that derrière, Xnr1, and Coco define a posttranscriptionally regulated signaling center, which is a necessary link in the signaling chain leading to an increased TGF-beta signal on the left side of the embryo...
  37. pmc From expression cloning to gene modeling: the development of Xenopus gene sequence resources
    Michael J Gilchrist
    Division of Systems Biology, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London, United Kingdom
    Genesis 50:143-54. 2012
    ..In addition, I demonstrate that the gaps in the current genome assembly create problems for the computational elucidation of gene sequences, and suggest some ways to ameliorate the effects of this...
  38. ncbi Crystal structure of a beta-catenin/Tcf complex
    T A Graham
    Department of Biological Structure University of Washington 98195, Seattle, WA, USA
    Cell 103:885-96. 2000
    ..The structural and mutagenesis data reveal a potential target for molecular drug design studies...
  39. pmc Greatwall maintains mitosis through regulation of PP2A
    Suzanne Vigneron
    Centre de Recherche de Biochimie Macromoleculaire, CNRS UMR 5237, IFR 122, Labellisée Ligue Nationale Contre le Cancer, Universités Montpellier 2 et 1, Montpellier, France
    EMBO J 28:2786-93. 2009
    ..These results suggest that mitotic entry and maintenance is not only mediated by the activation of cyclin B-Cdc2 but also by the regulation of PP2A by GW...
  40. pmc Roles of polymerization dynamics, opposed motors, and a tensile element in governing the length of Xenopus extract meiotic spindles
    T J Mitchison
    Marine Biological Laboratory, Woods Hole, MA 02543, USA
    Mol Biol Cell 16:3064-76. 2005
    ..The response to microtubule destabilization suggests that an unidentified tensile element acts in parallel with these conventional factors, generating spindle shortening force...
  41. ncbi Of Fox and Frogs: Fox (fork head/winged helix) transcription factors in Xenopus development
    Barbara S Pohl
    Abteilung Biochemie, , Albert Einstein Allee 11, Ulm D 89081, Germany
    Gene 344:21-32. 2005
    ..Finally, Fox proteins serve as mediators of signalling pathways and they might function as checkpoint molecules for the cross-regulatory interactions of different intracellular signal transduction chains...
  42. pmc Mcm10 and And-1/CTF4 recruit DNA polymerase alpha to chromatin for initiation of DNA replication
    Wenge Zhu
    Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    Genes Dev 21:2288-99. 2007
    ..The discovery also adds to the connection between replication initiation and sister chromatid cohesion...
  43. ncbi The Xenopus MEF2 gene family: evidence of a role for XMEF2C in larval tendon development
    Bruno Della Gaspera
    UMR 7060 CNRS, Equipe Biologie du Développement et de la Différenciation Neuromusculaire, Centre Universitaire des Saints Peres, 45, rue des Saints Pères, Universite Paris Descartes, F 75270 Paris Cedex 06, France
    Dev Biol 328:392-402. 2009
    ....
  44. ncbi Repression of nodal expression by maternal B1-type SOXs regulates germ layer formation in Xenopus and zebrafish
    Chi Zhang
    Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, 80309 0347, USA
    Dev Biol 273:23-37. 2004
    ..A mechanistically conserved system appears to act in a similar manner in the zebrafish...
  45. pmc Neural induction in the absence of mesoderm: beta-catenin-dependent expression of secreted BMP antagonists at the blastula stage in Xenopus
    O Wessely
    Howard Hughes Medical Institute, University of California, Los Angeles, California 90095-1662, USA
    Dev Biol 234:161-73. 2001
    ..The present results suggest an additional function for beta-Catenin, the early activation of expression of secreted BMP antagonists, such as Chordin, in a preorganizer region in the dorsal side of the Xenopus blastula...
  46. pmc The structural determinants of checkpoint activation
    Christina A MacDougall
    Department of Chemical and Systems Biology, Stanford University, Stanford, California 94305, USA
    Genes Dev 21:898-903. 2007
    ..These observations define the minimal DNA requirements for checkpoint activation and suggest that primed ssDNA represents a common checkpoint activating-structure formed following many types of damage...
  47. ncbi Xenopus Zic-related-1 and Sox-2, two factors induced by chordin, have distinct activities in the initiation of neural induction
    K Mizuseki
    Department of Biological Sciences, Kyoto University Faculty of Medicine, Yoshida, Sakyo, Kyoto 606, Japan
    Development 125:579-87. 1998
    ....
  48. pmc Translational control by cytoplasmic polyadenylation in Xenopus oocytes
    Helois E Radford
    School of Pharmacy, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, UK
    Biochim Biophys Acta 1779:217-29. 2008
    ..Finally we discuss some of the remaining questions regarding the mechanisms of translational regulation by cytoplasmic polyadenylation and give our view on where our knowledge is likely to be expanded in the near future...
  49. ncbi Msps/XMAP215 interacts with the centrosomal protein D-TACC to regulate microtubule behaviour
    M J Lee
    Department of Genetics, Wellcome CRC Institute, Tennis Court Road, Cambridge CB2 1QR, UK
    Nat Cell Biol 3:643-9. 2001
    ..We propose that D-TACC and Msps normally cooperate to stabilize centrosomal microtubules by binding to their minus ends and binding to their plus ends as they grow out from the centrosome...
  50. ncbi Direct binding of the PDZ domain of Dishevelled to a conserved internal sequence in the C-terminal region of Frizzled
    Hing C Wong
    Department of Structural Biology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
    Mol Cell 12:1251-60. 2003
    ..Together, our results identify a missing molecular connection within the Wnt pathway. Differences in the binding affinity of the Dvl PDZ domain and its binding partners may be important in regulating signal transduction by Dvl...
  51. ncbi Vertebrate hairy and Enhancer of split related proteins: transcriptional repressors regulating cellular differentiation and embryonic patterning
    R L Davis
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Oncogene 20:8342-57. 2001
    ....
  52. pmc Sfrp5 coordinates foregut specification and morphogenesis by antagonizing both canonical and noncanonical Wnt11 signaling
    Yan Li
    Cincinnati Children s Research Foundation and Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, Ohio 45229, USA
    Genes Dev 22:3050-63. 2008
    ..This novel mechanism coordinating canonical and noncanonical Wnt signaling may have broad implications for organogenesis and cancer...
  53. ncbi Regulation of Wnt signaling by Sox proteins: XSox17 alpha/beta and XSox3 physically interact with beta-catenin
    A M Zorn
    Wellcome Trust Cancer Research Campaign Institute of Cancer and Developmental Biology, Cambridge
    Mol Cell 4:487-98. 1999
    ..Two additional Sox proteins, XSox17 alpha and XSox3, likewise bind to beta-catenin and inhibit its TCF-mediated signaling activity. These results reveal an unexpected mechanism by which Sox proteins can modulate Wnt signaling pathways...
  54. ncbi Anterior specification of embryonic ectoderm: the role of the Xenopus cement gland-specific gene XAG-2
    F Aberger
    Institute of Genetics, University of Salzburg, Hellbrunnerstrasse 34, A 5020, Salzburg, Austria
    Mech Dev 72:115-30. 1998
    ..Based on these findings we propose a role for XAG-2 in the specification of dorsoanterior ectodermal fate, i.e. in the formation of cement gland and induction of forebrain fate of Xenopus...
  55. ncbi Nodal signals to Smads through Cripto-dependent and Cripto-independent mechanisms
    C Yeo
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell 7:949-57. 2001
    ..Inhibition appears to be mediated by heterodimerization between Nodal and BMPs, indicating that antagonism between Nodal and BMPs can occur at the level of dimeric ligand production...
  56. pmc Reconstitution of licensed replication origins on Xenopus sperm nuclei using purified proteins
    P J Gillespie
    CRC Chromosome Replication Research Group, Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, UK
    BMC Biochem 2:15. 2001
    ..Mcm2-7 are essential for DNA replication, but are displaced from origins as they initiate, thus ensuring that no origin fires more than once in a single cell cycle...
  57. ncbi Dickkopf-1 is a member of a new family of secreted proteins and functions in head induction
    A Glinka
    Division of Molecular Embryology, Deutsches Krebsforschungszentrum, Heidelberg, Germany
    Nature 391:357-62. 1998
    ..Injections of mRNA and antibody indicate that dkk-1 is sufficient and necessary to cause head induction. dkk-1 s a potent antagonist of Wnt signalling, suggesting that dkk genes encode a family of secreted Wnt inhibitors...
  58. ncbi XCDT1 is required for the assembly of pre-replicative complexes in Xenopus laevis
    D Maiorano
    Institute of Human Genetics, CNRS, Genome Dynamics and Development, Montpellier, France
    Nature 404:622-5. 2000
    ..These findings indicate that XCDT1 is an essential component of the system that regulates origins firing during S phase...
  59. ncbi Cerberus is a head-inducing secreted factor expressed in the anterior endoderm of Spemann's organizer
    T Bouwmeester
    Howard Hughes Medical Institute and Department of Biological Chemistry, University of California, Los Angeles, 90095 1737, USA
    Nature 382:595-601. 1996
    ..The results suggest a role for a molecule expressed in the anterior endoderm in the induction of head structures in the vertebrate embryo...
  60. ncbi Synergism between Pax-8 and lim-1 in embryonic kidney development
    T J Carroll
    Cell and Developmental Biology, University of Texas, Austin, Texas, 78712, USA
    Dev Biol 214:46-59. 1999
    ..Together these data indicate that the interaction between XPax-8 and Xlim-1 is a key early step in the establishment of the pronephric primordium...
  61. pmc GEMC1 is a TopBP1-interacting protein required for chromosomal DNA replication
    Alessia Balestrini
    Genome Stability, London Research Institute, Hertfordshire, UK
    Nat Cell Biol 12:484-91. 2010
    ..These data suggest that GEMC1 promotes initiation of chromosomal DNA replication in multicellular organisms by mediating TopBP1- and Cdk2-dependent recruitment of Cdc45 onto replication origins...
  62. ncbi Smad4 and FAST-1 in the assembly of activin-responsive factor
    X Chen
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115 5730, USA
    Nature 389:85-9. 1997
    ..These results indicate that Smad4 stabilizes a ligand-stimulated Smad2-FAST-1 complex as an active DNA-binding factor...
  63. pmc The N-terminal noncatalytic region of Xenopus RecQ4 is required for chromatin binding of DNA polymerase alpha in the initiation of DNA replication
    Kumiko Matsuno
    Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560 0043, Japan
    Mol Cell Biol 26:4843-52. 2006
    ..Our data suggest that RecQ4 performs an essential role in the assembly of replication machinery through interaction with Cut5 in vertebrates...
  64. ncbi Expression of Pax-3 is initiated in the early neural plate by posteriorizing signals produced by the organizer and by posterior non-axial mesoderm
    A G Bang
    Molecular Neurobiology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    Development 124:2075-85. 1997
    ....
  65. pmc RFX2 is broadly required for ciliogenesis during vertebrate development
    Mei I Chung
    Section of Molecular Cell and Developmental Biology, University of Texas at Austin, Austin, TX 78712, USA
    Dev Biol 363:155-65. 2012
    ..Furthermore, we show that Rfx2 is essential for expression of several ciliogenic genes, including TTC25, which we show here is required for ciliogenesis, HH signaling, and left-right patterning...
  66. ncbi Initiation of DNA replication requires the RECQL4 protein mutated in Rothmund-Thomson syndrome
    Mahesh N Sangrithi
    Cancer Research UK Department of Oncology, University of Cambridge, Hutchison MRC Research Centre, Hills Road, Cambridge CB2 2XZ, United Kingdom
    Cell 121:887-98. 2005
    ..This role connects defective replication initiation to a chromosome-fragility disorder...
  67. ncbi Structural basis for inhibition of the replication licensing factor Cdt1 by geminin
    Changwook Lee
    National Creative Research Center for Structural Biology and Department of Life Science, Pohang University of Science and Technology, Hyo ja dong, San31, Pohang, KyungBook, South Korea
    Nature 430:913-7. 2004
    ....
  68. ncbi Xenopus cadherin-11 restrains cranial neural crest migration and influences neural crest specification
    A Borchers
    Department of Genetics, Stanford University, CA 94305-5120, USA
    Development 128:3049-60. 2001
    ..Although Wnt/beta-catenin signalling is required to sustain twist expression during migration, it is not sufficient to block neural differentiation in non-migrating grafts...
  69. ncbi The Wnt/beta-catenin pathway posteriorizes neural tissue in Xenopus by an indirect mechanism requiring FGF signalling
    P M Domingos
    Division of Developmental Neurobiology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom
    Dev Biol 239:148-60. 2001
    ..We show that this posteriorizing activity occurs by an indirect and noncell-autonomous mechanism requiring FGF signalling...
  70. ncbi Inhibition of Wnt signaling pathway by a novel axin-binding protein
    T Kadoya
    Department of Biochemistry and Second Department of Surgery, Hiroshima University School of Medicine, 1 2 3 Kasumi, Minami Ku, Hiroshima 734 8551, PRESTO, Japan
    J Biol Chem 275:37030-7. 2000
    ..These results suggest that Axam regulates the Wnt signaling pathway negatively by inhibiting the binding of Dvl to Axin...
  71. ncbi Genetic control of hematopoietic development in Xenopus and zebrafish
    Aldo Ciau-Uitz
    MRC Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, UK
    Int J Dev Biol 54:1139-49. 2010
    ..In this review, we summarise our current knowledge of the cellular and genetic basis of ontogeny of the hematopoietic system in Xenopus and zebrafish embryos...
  72. pmc Protein arginine methyltransferase Prmt5-Mep50 methylates histones H2A and H4 and the histone chaperone nucleoplasmin in Xenopus laevis eggs
    Carola Wilczek
    Department of Biochemistry, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York 10461, USA
    J Biol Chem 286:42221-31. 2011
    ....
  73. ncbi Biochemical identification of Xenopus Pumilio as a sequence-specific cyclin B1 mRNA-binding protein that physically interacts with a Nanos homolog, Xcat-2, and a cytoplasmic polyadenylation element-binding protein
    S Nakahata
    Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060 0810, Japan
    J Biol Chem 276:20945-53. 2001
    ..These results also suggest the generality of the function of Pumilio as a translational regulator of dormant mRNAs in both invertebrates and vertebrates...
  74. pmc Cdc7-Drf1 kinase links chromosome cohesion to the initiation of DNA replication in Xenopus egg extracts
    Tatsuro S Takahashi
    Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 22:1894-905. 2008
    ..These results show that DDK is required to tether Scc2-Scc4 to pre-RCs, and they underscore the intimate link between early steps in DNA replication and cohesion...
  75. ncbi Paracrine and autocrine mechanisms of apelin signaling govern embryonic and tumor angiogenesis
    Roland E Kälin
    Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zurich, Wolfgang Pauli Strasse 10, CH 8093 Zurich, Switzerland
    Dev Biol 305:599-614. 2007
    ..Thus, our results define apelin and APJ as genes of potential diagnostic value and promising targets for the development of a new generation of anti-tumor angiogenic drugs...
  76. ncbi Control of gastrula cell motility by the Goosecoid/Mix.1/ Siamois network: basic patterns and paradoxical effects
    Olivia Luu
    Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada
    Dev Dyn 237:1307-20. 2008
    ..Fast and efficiently migrating multipolar cells and slow-moving polarized cells can be obtained by ectopic expression of these transcription factors in different combinations...
  77. pmc Wnt11-R signaling regulates a calcium sensitive EMT event essential for dorsal fin development of Xenopus
    Robert J Garriock
    Department of Cell Biology and Anatomy, University of Arizona Health Sciences Center, 1501 N Campbell Avenue, P O Box 245044, Tucson, AZ 85724, USA
    Dev Biol 304:127-40. 2007
    ..These studies are consistent with a model in which Wnt11-R signaling, via a downstream calcium pathway, regulates fin cell migration and, more generally, indicates a role for non-canonical Wnt signaling in regulation of EMT...
  78. ncbi Xenopus hairy2 functions in neural crest formation by maintaining cells in a mitotic and undifferentiated state
    Kan ichiro Nagatomo
    Department of Biology, Graduate School of Science, Osaka University, and JT Biohistory Research Hall, Osaka, Japan
    Dev Dyn 236:1475-83. 2007
    ..Furthermore, the depletion of Xhairy2 leads to the apoptosis of mitotic cells. Our results suggest that Xhairy2 functions in neural crest specification by maintaining cells in the mitotic and undifferentiated state...
  79. pmc Traf2- and Nck-interacting kinase is essential for canonical Wnt signaling in Xenopus axis formation
    Reiko Satow
    Chemotherapy Division, National Cancer Center Research Institute, Chuo Ku, Tokyo 104 0045, Japan
    J Biol Chem 285:26289-94. 2010
    ..These results demonstrate that XTNIK is an essential factor for the transcriptional activity of the beta-catenin-TCF complex and dorsal axis determination in Xenopus embryos...
  80. ncbi Zebrafish Rho kinase 2 acts downstream of Wnt11 to mediate cell polarity and effective convergence and extension movements
    Florence Marlow
    Department of Biological Sciences, Vanderbilt University, VU Station B 351634, Nashville, TN 37235 1634, USA
    Curr Biol 12:876-84. 2002
    ..Moreover, how defective mediolateral cell polarity impacts CE is not understood...
  81. ncbi Haploinsufficient phenotypes in Bmp4 heterozygous null mice and modification by mutations in Gli3 and Alx4
    N R Dunn
    Department of Cell Biology, Vanderbilt University Medical School, Nashville, Tennessee, 37232 2175, USA
    Dev Biol 188:235-47. 1997
    ..These genetic interactions suggest a model for the multigenic control of anterior digit patterning during vertebrate limb development...
  82. ncbi Frizzled7 mediates canonical Wnt signaling in neural crest induction
    Muhammad Abu-Elmagd
    School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK
    Dev Biol 298:285-98. 2006
    ..We therefore demonstrate that Xfz7 is regulated by BMP antagonism and is required for neural crest induction by Wnt in the developing vertebrate embryo...
  83. pmc CENP-E combines a slow, processive motor and a flexible coiled coil to produce an essential motile kinetochore tether
    Yumi Kim
    Ludwig Institute for Cancer Research, University of California, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 181:411-9. 2008
    ..We propose that the highly processive microtubule-dependent motor activity of CENP-E serves to power chromosome congression and provides a flexible, motile tether linking kinetochores to dynamic spindle microtubules...
  84. ncbi ATP4a is required for Wnt-dependent Foxj1 expression and leftward flow in Xenopus left-right development
    Peter Walentek
    University of Hohenheim, Institute of Zoology, Garbenstrasse 30, 70593 Stuttgart, Germany
    Cell Rep 1:516-27. 2012
    ..Specifically, ATP4 was required for Wnt/β-catenin-regulated Foxj1 induction and Wnt/PCP-dependent cilia polarization. Our work argues for evolutionary conservation of symmetry breakage in the vertebrates...
  85. ncbi The status of Wnt signalling regulates neural and epidermal fates in the chick embryo
    S I Wilson
    Department of Microbiology, , , Sweden
    Nature 411:325-30. 2001
    ..Conversely, a lack of exposure of epiblast cells to Wnt signals permits FGFs to induce a neural fate...
  86. pmc POU-V factors antagonize maternal VegT activity and beta-Catenin signaling in Xenopus embryos
    Ying Cao
    Institute of Biochemistry, University of Ulm, Albert Einstein Allee 11, 89081 Ulm, Germany
    EMBO J 26:2942-54. 2007
    ..We suggest that POU-V factors antagonize primary inducers to allow germ layer specification in a temporally and spatially coordinated manner...
  87. pmc Early, nonciliary role for microtubule proteins in left-right patterning is conserved across kingdoms
    Maria Lobikin
    Department of Biology, Center for Regenerative and Developmental Biology, Tufts University, Medford, MA 02155, USA
    Proc Natl Acad Sci U S A 109:12586-91. 2012
    ..The origin of laterality is cytoplasmic, ancient, and highly conserved across kingdoms, a fundamental feature of the cytoskeleton that underlies chirality in cells and multicellular organisms...
  88. ncbi Xenopus Staufen is a component of a ribonucleoprotein complex containing Vg1 RNA and kinesin
    Young J Yoon
    Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI 02912, USA
    Development 131:3035-45. 2004
    ..Our results suggest a central role for XStau in RNA localization in Xenopus oocytes, and provide evidence that Staufen is a conserved link between specific mRNAs and the RNA localization machinery...
  89. ncbi The Notch-effector HRT1 gene plays a role in glomerular development and patterning of the Xenopus pronephros anlagen
    Vincent Taelman
    Laboratoire d Embryologie Moleculaire, Universite Libre de Bruxelles, Institut de Biologie et de Médecine Moléculaires IBMM, Gosselies, Belgium
    Development 133:2961-71. 2006
    ..Together, our results provide evidence that XHRT1 plays an important role in glomerular development and early proximodistal patterning that is distinct from those of the other pronephric bHLH repressors...
  90. pmc Oct25 represses transcription of nodal/activin target genes by interaction with signal transducers during Xenopus gastrulation
    Ying Cao
    Institute of Biochemistry, University of Ulm, Ulm, Germany
    J Biol Chem 283:34168-77. 2008
    ..Our results provide a novel view in that Oct25 controls the nodal/activin pathway and thus maintains the undifferentiated state of embryonic cells in preventing them from premature differentiation...
  91. pmc Dynamic interactions of high Cdt1 and geminin levels regulate S phase in early Xenopus embryos
    Jolanta Kisielewska
    University of Newcastle, The Institute for Cell and Molecular Biosciences, Framlington Place, NE2 4HH, Newcastle upon Tyne, UK
    Development 139:63-74. 2012
    ....
  92. ncbi NLS-mediated NPC functions of the nucleoporin Pom121
    Sevil Yavuz
    European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    FEBS Lett 584:3292-8. 2010
    ..We propose that the NLS sites of Pom121 affect its function in NPC assembly both by influencing nucleoporin interactions and pore membrane structure...
  93. ncbi Serotonin signaling is required for Wnt-dependent GRP specification and leftward flow in Xenopus
    Tina Beyer
    Institute of Zoology, University of Hohenheim, Garbenstrasse 30, 70593 Stuttgart, Germany
    Curr Biol 22:33-9. 2012
    ..Our data confirm flow as primary mechanism of symmetry breakage and suggest a general role of serotonin as competence factor for Wnt signaling during axis formation in Xenopus...
  94. ncbi Kinesin II mediates Vg1 mRNA transport in Xenopus oocytes
    J Nicholas Betley
    Department of Biology, Boston University, Boston, MA, USA
    Curr Biol 14:219-24. 2004
    ..These data support a model in which kinesin II mediates the transport of specific RNA complexes destined for the vegetal cortex...
  95. ncbi Two novel nodal-related genes initiate early inductive events in Xenopus Nieuwkoop center
    S Takahashi
    Department of Life Sciences Biology, The University of Tokyo, Tokyo 153 8902, Japan
    Development 127:5319-29. 2000
    ..The expression of Xnr5 and Xnr6 is regulated by maternal VegT and (beta)-catenin, but does not require TGF-(beta) signaling. Therefore, expression of Xnr5 and Xnr6 is controlled by different mechanisms from other Xnr family genes...
  96. ncbi Dynamic patterns of gene expression in the developing pronephros of Xenopus laevis
    T J Carroll
    Department of Zoology, University of Texas, Austin 78712, USA
    Dev Genet 24:199-207. 1999
    ..We describe restrictions of these gene expression patterns within the pronephros which indicate a model for the partitioning of the common pronephric anlage into its three component parts--the tubules, the glomus, and the duct...
  97. pmc Temporal profiling of the chromatin proteome reveals system-wide responses to replication inhibition
    Guennadi A Khoudoli
    Wellcome Trust Centre for Gene Regulation and Expression, University of Dundee, Dundee DD1 5EH, United Kingdom
    Curr Biol 18:838-43. 2008
    ....
  98. pmc H,K-ATPase protein localization and Kir4.1 function reveal concordance of three axes during early determination of left-right asymmetry
    Sherry Aw
    Forsyth Institute, Forsyth Center for Regenerative and Developmental Biology, 140 The Fenway, Boston, MA 02115, USA
    Mech Dev 125:353-72. 2008
    ....
  99. pmc Convergence of a head-field selector Otx2 and Notch signaling: a mechanism for lens specification
    Hajime Ogino
    Department of Biology, University of Virginia, Charlottesville, VA 22904, USA
    Development 135:249-58. 2008
    ..This concept is also consistent with classical embryological studies of many organ systems involving a ;multiple-step induction'...
  100. pmc Xenopus DNA2 is a helicase/nuclease that is found in complexes with replication proteins And-1/Ctf4 and Mcm10 and DSB response proteins Nbs1 and ATM
    Karen E Wawrousek
    Division of Biology, California Institute of Technology, Pasadena, CA, USA
    Cell Cycle 9:1156-66. 2010
    ..The nuclease activity of Mre11 is required, since its inhibition delays both full Dna2 recruitment and resection. Dna2 depletion inhibits but does not block resection, and Chk1 and Chk2 induction occurs in the absence of Dna2...
  101. ncbi Structural basis of Aurora-A activation by TPX2 at the mitotic spindle
    Richard Bayliss
    Structural and Computational Biology Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117, Heidelberg, Germany
    Mol Cell 12:851-62. 2003
    ..The recognition between Aurora-A and TPX2 resembles that between the cAPK catalytic core and its flanking regions, suggesting this molecular mechanism may be a recurring theme in kinase regulation...

Research Grants65

  1. MARCKS Phosphorylation in Growth Cone Signaling
    JESSE GATLIN; Fiscal Year: 2004
    ..Functional consequences of aberrant PKC signaling will be determined by observing the turning response of growth cones from DRG neurons expressing a dominant-negative, phosphorylation-deficient MARCKS mutant. ..
  2. Computation tools for analyzing gene expression data
    Naama Barkai; Fiscal Year: 2004
    ..Kinetic microarray experiments that highlight specific features of the transcriptional network will be designed, performed and analyzed. Methods for extracting regulatory relationships from these experiments will be developed. ..
  3. Analysis of Early Wing Vein Development in Drosophila
    Ethan Bier; Fiscal Year: 2007
    ..Thus, knowledge gained from these studies should have broad applications to understanding vertebrate development as well as offering possible insights into mechanisms of human disease. ..
  4. Wnt Pathway Regulation in Embryos and Cells
    David Kimelman; Fiscal Year: 2009
    ..Since the Wnt pathway plays key roles in vertebrate development and oncogenesis, these studies will be important in advancing knowledge of the molecular basis of birth defects and cancer. ..
  5. Combinatorial signaling in zebrafish development
    David Kimelman; Fiscal Year: 2005
    ..abstract_text> ..
  6. EUKARYOTIC HYALURONAN SYNTHASES
    PAUL DEANGELIS; Fiscal Year: 2001
    ..The essential residues identified in aim 3 will also be confirmed by mutagenesis studies. The enzymological and structural information may allow the generation of a working model describing the catalytic mechanism of these enzymes. ..
  7. Probing Cell Division with Synthetic Chemistry
    Timothy Mitchison; Fiscal Year: 2008
    ..Having found small molecule tools that perturb cell division by novel mechanisms, we will test their ability to kill cancer cells in vitro. ..
  8. ACTIN DYNAMICS AND CELL LOCOMOTION
    Timothy Mitchison; Fiscal Year: 2004
    ..For perturbation experiments we will use protein tools, and also cell permeable chemicals coming out of a screening effort at the Harvard Institute of Chemistry and Cell Biology (ICCB). ..
  9. Cytoskeleton polymerization dynamics in the cell cycle
    Timothy J Mitchison; Fiscal Year: 2010
    ..Understanding midbody assembles will reveal principles for generating asymmetric assemblies in the cytoplasm, and will tell us how the cleavage furrow is positioned. ..
  10. ELECTRON MICROSCOPE
    Timothy Mitchison; Fiscal Year: 2004
    ..The automated controls and digital image acquisition will also be optimal for introducing new users to electron microscopy and for minimizing maintenance requirements that might arise from operator inexperience. ..
  11. Conference on Wnt and Beta Catenin Signaling
    Randall Moon; Fiscal Year: 2002
    ..Given the explosive growth in research in this field owing to its connection to various cancers, it is extremely timely to have a meeting with the joint focus on the roles of Wnt and B-catenin signaling in both development and disease. ..
  12. ErbB signaling in vertebrate morphogenesis
    Chenbei Chang; Fiscal Year: 2010
    ..abstract_text> ..
  13. Wnt and beta-Catenin Signaling in Development/Disease
    Mariann Bienz; Fiscal Year: 2006
    ..Basic researchers and the pharmaceutical and biotechnology industry are invited to discuss how to exploit model systems and how to translate knowledge from these into drug development. [unreadable] [unreadable]..
  14. The Role of Eomesodermin in Cardiac Development
    Kenneth Ryan; Fiscal Year: 2007
    ..AIM 4. In situ hybridization is used to determine the full range of cardiac cell types expressing Eomes; a conditional dominant-negative isoform of Eomes examines the requirement for Eomes in the heart. ..
  15. Twisted Gastrulation Gene in Vertebrate Development
    Chenbei Chang; Fiscal Year: 2007
    ..abstract_text> ..
  16. Toxicant Target Genes in Xenopus and Zebrafish Embryos
    Jeremy Green; Fiscal Year: 2004
    ..These experiments will generate useful comparative information as well as lay the foundation for illuminating the mechanisms of developmental toxicity at the molecular and genomic levels. ..
  17. Acquisition of a High-throughput Confocal Imaging System
    Claire Walczak; Fiscal Year: 2008
    ..Overall the proposed instrumentation would have a significant impact on the research approaches and progress made by life sciences researchers at our university. [unreadable] [unreadable] [unreadable]..
  18. Left-Right Axis Determination and Cardiac Development
    ANN RAMSDELL; Fiscal Year: 2005
    ..The long-term significance of the work will be to identify genes and inductive processes that may result in congenital cardiac (and other) LR defects if affected by genetic or environmental perturbations. ..
  19. Retinal Circadian Signaling in a Transgenic Model
    Allan Wiechmann; Fiscal Year: 2004
    ..Discovering the molecular mechanisms of melatonin action may therefore provide a greater understanding of the role of circadian signals in environmentally- and genetically- induced photoreceptor degenerations. ..
  20. SPINDLE ASSEMBLY CHECKPOINT IN VERTEBRATES AND YEAST
    Rey Huei Chen; Fiscal Year: 2003
    ..The knowledge will then be applied to the question of how the deregulation of the checkpoint function may contribute to cancer progression. ..
  21. PATTERNING BY TGF BETA IN EARLY XENOPUS EMBRYOS
    Jeremy Green; Fiscal Year: 2002
    ..He also proposes to determine the effects on different processes in Xenopus development when different TGF-SM pathways are activated or blocked. ..
  22. Intrinsic Spindle Mechanisms to Insure Bipolarity
    JESSE GATLIN; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  23. Chemical genetic analysis of intracellular Transport
    Tarun Kapoor; Fiscal Year: 2007
    ..It is likely that these small molecules will lead to the identification of new targets for therapeutic agents and improved treatments of human diseases. ..
  24. Anti-Adenosine Therapy of Neonatal Brain Injury
    Scott Rivkees; Fiscal Year: 2007
    ..It is anticipated that these studies will lead to novel and practical strategies for directly preventing brain injury. [unreadable] [unreadable]..
  25. From embryo to adult: mechanisms of fetal programming
    Tom Fleming; Fiscal Year: 2006
    ..Our multidisciplinary approach has been designed to provide the first integrative assessment of dietary programming mechanisms, from embryo to adult. ..
  26. T cell immune response to group A streptococci in NALT
    Paul Cleary; Fiscal Year: 2008
    ..Experiments will determine impact of infection on the antigen specific T cell response in NALT and other lymphoid organs. ..
  27. Regulation of TGF-beta Signaling and Embryonic Development by GTPases
    Gerald H Thomsen; Fiscal Year: 2010
    ..Our studies will provide crucial information about GTPBPs which may eventually translate into clinical treatments for diseases caused by abnormal TGF[unreadable] or GTPBP signaling. ..
  28. ROLE OF NON-CLASSICAL MHC CLASS I AND HSPs IN IMMUNITY
    Jacques Robert; Fiscal Year: 2009
    ..Do hsp70 and gp96 generate similar anti-tumor CCU-CTL effectors? Aim 3. How important is the hsp-generated CCU-CTL responses in tumor immunity in vivo? Aim 4. Do CCU-CTLs interact with class Ib molecules? ..
  29. FASEB Summer Research Conference on "The Biology and Chemistry of Vision"
    JOSEPH BESHARSE; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable] [unreadable]..
  30. The Role of MeCP2 in L1 Neuronal Retrotransposition
    Fred Gage; Fiscal Year: 2008
    ..The validation of this hypothesis will certainly open new potential possibilities for therapeutic interventions for RTT and other neurological diseases. [unreadable] [unreadable] [unreadable]..
  31. Mechanisms of Microtubule Dynamic Turnover
    Patricia Wadsworth; Fiscal Year: 2009
    ..Learning how normal cells construct a mitotic spindle is an important first step towards understanding defective cell division in cancer. ..
  32. Biogenesis of Small RNAs
    SANDRA WOLIN; Fiscal Year: 2009
    ..By understanding these processes, it may be possible to design treatments for diseases caused by noncoding RNAs that do not function properly. ..
  33. MOLECULAR MECHANISMS OF VIRAL MEMBRANE FUSION PROTEINS
    Judith White; Fiscal Year: 2009
    ..g. influenza, West Nile, and Ebola viruses). The proposed work will aid these important efforts. ..
  34. Development of Synaptic Specificity in the Mammalian Visual System
    Ben Barres; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  35. TRAF4 in TGF-beta Signaling and Embryonic Development
    GERALD THOMSEN; Fiscal Year: 2009
    ....
  36. Understanding Head Development: A Segmental Groundplan of Vertebrates
    Thomas Schilling; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  37. RNA and the Etiology of Disease
    Joel Richter; Fiscal Year: 2008
    ..The meeting is cross-disciplinary in its approach, which is essential for combating human disease. [unreadable] [unreadable] [unreadable] [unreadable]..
  38. Genetic analysis of ovary development and oogenesis
    Mary Mullins; Fiscal Year: 2009
    ..Identification of the mutant zebrafish genes will provide candidate genes responsible for the human defects, which could ultimately lead to genetic testing or therapeutic intervention. ..
  39. Cell Genesis in the Intact and Injured Substantia Nigra
    Fred Gage; Fiscal Year: 2005
    ..The suggested experiments will provide the groundwork for possible future strategies in which we will try to manipulate cell genesis in the adult SN in order to restore the function of the injured adult SN. ..
  40. Cyclin Control DNA Replication and Mitosis
    Peter Jackson; Fiscal Year: 2005
    ..These aims will both map Cdc 14-regulated pathways and begin to define the critical Cdk and Cdcl4 targets for regulating steps in mitosis and genome stability. ..
  41. Q3DM/Beckman Coulter IC 100 Automated Microscope System
    Mark Mercola; Fiscal Year: 2005
    ....
  42. TRANSLATIONAL CONTROL MEDIATED BY MRNA STRUCTURE
    Marilyn Kozak; Fiscal Year: 2002
    ..The reason why 40S ribosome/factor complexes protect "extra" sequences, which are accessible to RNase in 80S initiation complexes, will be studied. ..
  43. SIGNALING PATHWAYS THAT REGULATE MITOSIS
    THOMAS GUADAGNO; Fiscal Year: 2005
    ..Ultimately, we hope to establish whether defects in MAP kinase regulation during mitosis may contribute to genomic instabilities associated with tumor progression. ..
  44. A-1 Adenosine Receptor Action in Developing Heart
    Scott Rivkees; Fiscal Year: 2005
    ..We anticipate that these studies will identify Rho and ROCK as critical signaling molecules in heart development and reveal that cardiac GTPase activity transduces the effects of adenosine. ..
  45. The Role of Adenosine in Hypoglycemic Brain Injury
    Scott Rivkees; Fiscal Year: 2003
    ..We anticipate that these studies will yield new insights into the mechanisms of hypoglycemic brain injury and may lead to the development of practical strategies for reducing hypoglycemic brain injury. ..
  46. LEICA TCS SPII SPECTRAL CONFOCAL MICROSCOPE
    JOSEPH BESHARSE; Fiscal Year: 2001
    ..After the project period, the long term care of the instrument for use of NIH funded investigators will be placed under the supervision of the existing imaging core facility. ..
  47. THE ROLE OF UFD1 AND CDC45 IN NEURAL CREST DEVELOPMENT
    Vidu Garg; Fiscal Year: 2004
    ..The function of Cdc45 will be examined in vivo in chick embryos by retrovirally mediated alteration of gene expression. A yeast two hybrid assay will be utilized to determine if Ufdl, Cdc45, and Hira interact with one another. ..
  48. Notch function in heart and kidney organogenesis
    Mark Mercola; Fiscal Year: 2004
    ..Knowledge of Notch function and the key downstream regulatory proteins should provide molecular insights that may be of therapeutic benefit for disease and congenital disorders. ..
  49. CELL CYCLE CONTROL BY THE CAMP PKA SIGNAL TRANSDUCTION P
    Jean Gautier; Fiscal Year: 2002
    ..This unique approach will help elucidate how PKA coordinates cell cycle progression in concert with other protein kinases in more complex, i.e. growth regulated systems. ..
  50. Mechanism of Poly-ADP-ribose function in the spindle
    Paul Chang; Fiscal Year: 2005
    ..Preliminary experiments demonstrate a requirement for PAR in spindle function. Here, I propose to examine the mechanism by which PAR acts in the mitotic spindle. ..
  51. EARLY EVENTS IN NEURAL AND HOMEOGENETIC INDUCTION
    Claudio Stern; Fiscal Year: 2005
    ..D. To explore the possible roles of 6 newly-identified candidate secreted factors in the early steps of neural induction and homeogenetic induction. ..
  52. CONFOCAL MICROSCOPY SYSTEM
    EARL GODFREY; Fiscal Year: 2001
    ..This instrument would serve as the centerpiece of a modern imaging facility to facilitate NIH funded research at EVMS. ..
  53. MOLECULAR GENETIC ANALYSIS OF ASYMMETRIC CELL DIVISIONS
    Chris Doe; Fiscal Year: 2002
    ..We will do RNA and protein localization studies; our collaborator, Dr. G. Oliver will generate and assay the gene knock-out mice. ..
  54. Translational Research of AAV-IGF-1 Gene Therapy for ALS
    Fred Gage; Fiscal Year: 2006
    ..We aim to complete further efficacy studies as well safety and toxicity studies required for the submission of an Investigational New Drug Application (IND) for a clinical study of AAV-IGF-1 in adults with ALS. [unreadable] [unreadable]..
  55. Mechanisms of Growth Cone Turning in Diffusible Gradient
    James Zheng; Fiscal Year: 2006
    ....
  56. Mature Brain Astrocyte Functions in Health and Disease
    Ben Barres; Fiscal Year: 2006
    ..Understanding reactive gliosis may also lead to new treatments, as it contributes to the pathophysiology of neurodegenerative diseases, stroke, epilepsy, and limits regenerative CNS repair. [unreadable] [unreadable]..
  57. REGULATION OF BETA-CATENIN NUCLEARIZATION
    CHARLES ETTENSOHN; Fiscal Year: 2006
    ..In addition, using dominant negative constructs and morpholinos, we will examine the developmental function of two regulators of GSK3 activity, disheveled and Akt/PKB. ..
  58. STREPTOCOCCAL INACTIVATOR IN HUMAN CHEMOATTRACTANTS
    Paul Cleary; Fiscal Year: 2006
    ..New data indicates that the C5a peptide required for successful colonization and persistence of streptococci in nasal associated lymphoid tissue. ..
  59. INTRACELLULAR INFECTION BY INVASIVE GROUP A STREPTOCOCCI
    Paul Cleary; Fiscal Year: 2006
    ..Information gained from this study may identify new targets for antibiotic development, and increase our understanding of the mechanisms by which this pathogen persists in human populations. ..
  60. Mechanisms of Processivity in Molecular Motors
    Steven Rosenfeld; Fiscal Year: 2008
    ..Furthermore, if successful, this work will support the argument that understanding how a motor works in vitro as an enzyme can provide valuable insights into how it works in vivo in the cell. ..
  61. Development of High Throughput Screening Assays (RMI)
    Steven Rosenfeld; Fiscal Year: 2004
    ..This work will serve as the basis for a future extramural funding initiative designed to take promising compounds identified by this approach into pre-clinical glioma model systems, and ultimately, into clinical trial. ..
  62. 2006 Biophysical Discussions - Molecuar Motors: Point Counterpoint
    Steven Rosenfeld; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  63. Genetic Analysis of Bacterial Toxins in Drosophia
    Ethan Bier; Fiscal Year: 2005
    ..DN alleles 2-2: Characterize molecular lesion in DN-toxin NOVA alleles 2-3: Confirm that DN-toxin alleles interfere with wild-type toxin gene function 2-4: Determine whether DN-toxin alleles function in mammalian cells ..