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| polylysineSummarySummary: A peptide which is a homopolymer of lysine. Top Publications
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Publications
Adhesion and proliferation of cells on new polymers modified biomaterialsS Lakard
Laboratory of Chemistry Materials and Interfaces, UFR Sciences et Technique, University of Franche Comte, 16 route de Gray, Batiment Propedeutique, 25030 Besancon, France
Bioelectrochemistry 62:19-27. 2004..Consequently, cells have a greater fold expansion on PEI and PPI than on PPPD, PPy or glass and FTO uncoated. From these results, we deduce that PEI and PPI can be useful as coating surface to cultivate neuronal cells...- A tetra(L-lysine)-grafted poly(organophosphazene) for gene deliveryYong Joo Jun
Division of Nano Science BK 21, Ewha Womans University, 11 1 Daehyun Dong, Seodaemun gu, Seoul 120 750, Republic of Korea
Bioorg Med Chem Lett 17:2975-8. 2007.... 
Physicochemical properties and antimicrobial efficacy of electrostatic complexes based on cationic ε-polylysine and anionic pectinYuhua Chang
Department of Food Science, University of Massachusetts, Amherst, Massachusetts 01003, USA
J Agric Food Chem 59:6776-82. 2011b>Polylysine (ε-PL) is a food-grade cationic antimicrobial that is highly effective against a wide range of food pathogens and spoilage organisms...- Characterization of biophysical and metabolic properties of cells labeled with superparamagnetic iron oxide nanoparticles and transfection agent for cellular MR imagingAli S Arbab
Experimental Neuroimaging Section, Laboratory of Diagnostic Radiology Research, National Institutes of Health, 10 Center Dr, Rm B1N256, Bethesda, MD 20892, USA
Radiology 229:838-46. 2003..To evaluate the effect of using the ferumoxides-poly-l-lysine (PLL) complex for magnetic cell labeling on the long-term viability, function, metabolism, and iron utilization of mammalian cells... - Effect of human stem cells labeled with ferumoxides-poly-L-lysine on hematologic and biochemical measurements in ratsGene T Yocum
Experimental Neuroimaging Section, Laboratory of Diagnostic Radiology Research, Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA
Radiology 235:547-52. 2005..CONCLUSION: Results indicate that infusing cells that are magnetically labeled with ferumoxides-PLL complex into rats does not alter biochemical or hematologic measures of organ function in a clinically relevant or preclusive manner... - Poly(L-lysine)-modified iron oxide nanoparticles for stem cell labelingMichal Babic
Institute of Macromolecular Chemistry, v v i, Academy of Sciences of the Czech Republic, Heyrovsky Sq 2, 162 06 Prague 6, Czech Republic
Bioconjug Chem 19:740-50. 2008..In comparison with Endorem, nanoparticles modified with PLL of an optimum molecular weight demonstrated a higher efficiency of intracellular uptake by MSC cells... - Safety, tolerability, and pharmacokinetics of SPL7013 gel (VivaGel): a dose ranging, phase I studyJohn O'Loughlin
Royal Adelaide Hospital, Adelaide, Australia
Sex Transm Dis 37:100-4. 2010..To evaluate safety, tolerability and systemic pharmacokinetics of escalating doses of SPL7013 Gel in healthy women... - Polylysine-modified PEG-based hydrogels to enhance the neuro-electrode interfaceShreyas S Rao
William G Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 W 19th Avenue, Columbus, OH 43210, USA
J Biomater Sci Polym Ed 22:611-25. 2011..In particular, polylysine-modified PEG-based hydrogels were synthesized, characterized and shown to promote neural adhesion using a PC12 .. - On the kinetics of polyplex endocytic trafficking: implications for gene delivery vector designM Laird Forrest
Department of Chemical and Biomolecular Engineering, University of Illinois, Urbana, Illinois 61801, USA
Mol Ther 6:57-66. 2002..Additionally, PEI does not appear to buffer endocytic compartments, but requires exposure to an acidic environment for efficient gene delivery... - Characterization of interpolyelectrolyte complexes between double-stranded DNA and polylysine comb-type copolymers having hydrophilic side chainsA Maruyama
Department of Biomolecular Engineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori, Yokohama, Japan
Bioconjug Chem 9:292-9. 1998.... - PEGylation of polylysine dendrimers improves absorption and lymphatic targeting following SC administration in ratsLisa M Kaminskas
Drug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University Parkville Campus 381 Royal Pde, Parkville, VIC 3052, Australia
J Control Release 140:108-16. 2009b>Polylysine dendrimers have potential as highly flexible, biodegradable nanoparticular carriers that may also promote lymphatic transport... - Potential novel drug carriers for inner ear treatment: hyperbranched polylysine and lipid nanocapsulesVerena Scheper
Department of Otolaryngology, Medical University Hannover, Carl Neuberg Str 1, 30625 Hannover, Germany
Nanomedicine (Lond) 4:623-35. 2009Treatment of sensorineural hearing loss could be advanced using novel drug carriers such as hyperbranched polylysine (HBPL) or lipid nanocapsules (LNCs)... - Double genetic modification of adenovirus fiber with RGD polylysine motifs significantly enhances gene transfer to isolated human pancreatic isletsJuan L Contreras
Department of Surgery, University of Alabama at Birmingham, Birmingham, AL 35294, USA
Transplantation 76:252-61. 2003..Because the arginine-glycine-aspartame (RGD) and polylysine (pK7) motifs have been shown to enhance Ad5 infection through an Ad5 receptor-independent pathway, we .. - Clinically applicable labeling of mammalian and stem cells by combining superparamagnetic iron oxides and transfection agentsJoseph A Frank
Laboratory of Diagnostic Radiology Research, Clinical Center, National Institutes of Health, Bldg 10, Rm B1N256, 10 Center Dr, MSC 1074, Bethesda, MD 20892 1074, USA
Radiology 228:480-7. 2003..To label mammalian and stem cells by combining commercially available transfection agents (TAs) with superparamagnetic iron oxide (SPIO) magnetic resonance (MR) imaging contrast agents... - Use of ADME studies to confirm the safety of epsilon-polylysine as a preservative in foodJun Hiraki
Chisso Corporation, Yokohama, Japan
Regul Toxicol Pharmacol 37:328-40. 2003Epsilon-polylysine is a homopolymer of L-lysine, containing approximately 30 L-lysine subunits, as synthesized in aerobic bacterial fermentation by Streptomyces albulus... - Evaluation of dendrimer SPL7013, a lead microbicide candidate against herpes simplex virusesEdwin Gong
Viridae Clinical Sciences Inc, Vancouver, BC, Canada
Antiviral Res 68:139-46. 2005..The 50% cytotoxic concentrations (CC(50)) in all cases were greater than 10,000 microg/ml. Our data indicate that SPL7013 is a promising candidate for development as a vaginal microbicide and a therapeutic agent... - DNA strand exchange stimulated by spontaneous complex formation with cationic comb-type copolymerWon Jong Kim
Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, and PRESTO, Japan Science and Technology Corporation, Midori, Yokohama 226-8501, Japan
J Am Chem Soc 124:12676-7. 2002..CCCs stabilize not only matured hybrids but also the nucleation complex to accelerate hybridization... - Double modification of adenovirus fiber with RGD and polylysine motifs improves coxsackievirus-adenovirus receptor-independent gene transfer efficiencyHongju Wu
Division of Human Gene Therapy, Department of Medicine, Gene Therapy Center, University of Alabama at Birmingham, Birmingham, AL 35294-2172, USA
Hum Gene Ther 13:1647-53. 2002..Two motifs, RGD and polylysine (pK7), have been shown to enhance Ad5 infection via CAR-independent pathways when incorporated into fiber .. - Microencapsulation of cells producing therapeutic proteins: optimizing cell growth and secretionAnne Mari Rokstad
Institute of Cancer Research and Molecular Biology, Norwegian University of Science and Technology, Trondheim
Cell Transplant 11:313-24. 2002.... - Systemic circulation of poly(L-lysine)/DNA vectors is influenced by polycation molecular weight and type of DNA: differential circulation in mice and rats and the implications for human gene therapyC M Ward
CRC Institute for Cancer Studies, University of Birmingham, United Kingdom
Blood 97:2221-9. 2001..Analysis using human blood in vitro shows no hemolysis, but both types of complexes fix complement and bind IgG, suggesting that pLL/DNA complexes may be rapidly cleared from the human circulation... - Polylysine induces an antiparallel actin dimer that nucleates filament assembly: crystal structure at 3.5-A resolutionMichael R Bubb
Research Service, Malcom Randall Department of Veterans Affairs Medical Center and the University of Florida College of Medicine, Gainesville, Florida 32608, USA
J Biol Chem 277:20999-1006. 2002..We now show that latrunculin A, a marine natural product that inhibits actin polymerization, arrests polylysine-induced nucleation at the level of an antiparallel dimer, resulting in its accumulation... - PEG grafted polylysine with fusogenic peptide for gene delivery: high transfection efficiency with low cytotoxicityHaeshin Lee
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Kusong-dong, Yusong-gu, 305-701, Taejon, South Korea
J Control Release 79:283-91. 2002..This suggests that maintaining the size of DNA/polymer complexes after KALA coating plays an important role in gene transfection. KALA/DNA/PEG-g-PLL complexes exhibited lower cytotoxicity compared with other polymer/DNA complexes... - Assaying phosphatidylinositol bisphosphate regulation of potassium channelsTibor Rohacs
Department of Physiology and Biophysics, Mount Sinai School of Medicine, New York, New York 10029, USA
Methods Enzymol 345:71-92. 2002 - Inhibition of lipases by epsilon-polylysineTakahiro Tsujita
Central Research Laboratory, School of Medicine, Ehime University, Shigenobu, Onsen gun, Ehime 791 0295, Japan
J Lipid Res 44:2278-86. 2003Oral administration of epsilon-polylysine to rats reduced the peak plasma triacylglycerol concentration... - Intracytoplasmic tagging of cells with ferumoxides and transfection agent for cellular magnetic resonance imaging after cell transplantation: methods and techniquesAli S Arbab
Experimental Neuroimaging Section, Laboratory of Diagnostic Radiology Research, National Institutes of Health, Bethesda, MD 20892, USA
Transplantation 76:1123-30. 2003..The purpose of this study is to present protocols using SPIO and a polycationic transfection agent for magnetic labeling of cells as a basis for cellular MRI... - Cell surface nucleolin serves as receptor for DNA nanoparticles composed of pegylated polylysine and DNAXuguang Chen
Department of Case Western Reserve University School of Medicine, Cleveland, Ohio 44106, USA
Mol Ther 16:333-42. 2008..Therefore, the data strongly suggest that cell surface nucleolin serves as a receptor for DNA nanoparticles, and that nucleolin is essential for internalization and/or transport of the nanoparticles from cell surface to the nucleus... - Spectroscopic investigation of cationic comb-type copolymers/DNA interaction: interpolyelectrolyte complex enhancement synchronized with DNA hybridizationYuichi Sato
Precursory Research for Embryonic Science and Technology PRESTO and Core Research for Evolutional Science and Technology CREST, Japan Science and Technology Agency, 4 1 8 Honcho, Kawaguchi, Saitama 332 0012, Japan
Langmuir 23:65-9. 2007..The IPEC formation of CCCs with DNA, however, seems highly sensitive to the coil-helix transition of the DNA... 
C2B polylysine motif of synaptotagmin facilitates a Ca2+-independent stage of synaptic vesicle priming in vivoCarin A Loewen
Molecular, Cellular, and Integrative Neuroscience Program, Department of Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA
Mol Biol Cell 17:5211-26. 2006..I, a synaptic vesicle protein required for efficient synaptic transmission, contains a highly conserved polylysine motif necessary for function...- Polylysine-functionalised thermoresponsive chitosan hydrogel for neural tissue engineeringK E Crompton
School of Physics, Department of Materials Engineering, Monash University, Wellington Rd, Clayton, VIC 3800, Australia
Biomaterials 28:441-9. 2007..The results show that thermally responsive chitosan/GP hydrogels provide a suitable 3D scaffolding environment for neural tissue engineering... - Preclinical safety and efficacy assessments of dendrimer-based (SPL7013) microbicide gel formulations in a nonhuman primate modelD L Patton
Department of Obstetrics and Gynecology, Box 356460, University of Washington, Seattle, WA 98195 6460, USA
Antimicrob Agents Chemother 50:1696-700. 2006..However, a single intravaginal application of the 3% (wt/wt) SPL7013 gel did not provide protection from the acquisition of cervical chlamydial infection... - Alginate-PLL microencapsulation: effect on the differentiation of embryonic stem cells into hepatocytesTim Maguire
Department of Biomedical Engineering, Rutgers University, Piscataway, New Jersey 08854, USA
Biotechnol Bioeng 93:581-91. 2006..This system may ultimately assist in developing scalable stem cell differentiation strategies... - Investigation of a new microcapsule membrane combining alginate, chitosan, polyethylene glycol and poly-L-lysine for cell transplantation applicationsT Haque
Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine, McGill University, Montreal, Quebec, Canada
Int J Artif Organs 28:631-7. 2005..The study demonstrated that the new membrane can be used for cell entrapment. However, further investigations are needed to assess its potential for long term transplantation and usage in the development of bioartificial organs... - In vitro study of alginate-chitosan microcapsules: an alternative to liver cell transplants for the treatment of liver failureTasima Haque
Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering and Organs Research Center, Faculty of Medicine, McGill University, 3775 University Street, Montreal, Quebec, Canada H3A 2B4
Biotechnol Lett 27:317-22. 2005..The AC microcapsule has several advantages compared to more widely used alginate-poly-L-lysine (APA) microcapsules for the application of cell therapy... - SPL7013 gel as a topical microbicide for prevention of vaginal transmission of SHIV89.6P in macaquesYong-Hou Jiang
Washington National Primate Research Center, University of Washington, Seattle, Washington 98195, USA
AIDS Res Hum Retroviruses 21:207-13. 2005..These results suggest that SPL7013 gel may be a promising anti-HIV microbicide formulation for further evaluation... - The effect of backbone structure on polycation comb-type copolymer/DNA interactions and the molecular assembly of DNAYu-ichi Sato
PRESTO, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan
Biomaterials 26:703-11. 2005..The study suggested that tailor-made design of "functional polycounterion" is a strategy to engineer molecular assembling of DNA... - Comparison of transfection agents in forming complexes with ferumoxides, cell labeling efficiency, and cellular viabilityAli Syed Arbab
Laboratory of Diagnostic Radiology Research, National Institutes of Health, Bethesda, MD 20892, USA
Mol Imaging 3:24-32. 2004..Concentrations of 25 to 50 microg/mL of FEs complexed to TAs in media resulted in sufficient endocytosis of the SPIO into endosomes to detect cells on cellular magnetic resonance imaging... - Poly(L-lysine)-GRGDS as a biomimetic surface modifier for poly(lactic acid)R A Quirk
School of Pharmaceutical Sciences, The University of Nottingham, University Park, UK
Biomaterials 22:865-72. 2001..Potentially, this is a flexible method of surface modification that can engineer many different types of tissue engineering scaffolds with a variety of biomolecules, thus allowing initial cell adhesion to be controlled... - Mechanistic differences in DNA nanoparticle formation in the presence of oligolysines and poly-L-lysineIrina Nayvelt
Department of Medicine, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, New Brunswick, New Jersey 08903, USA
Biomacromolecules 8:477-84. 2007..The DNA melting temperature increased with oligolysine concentration. These results indicate distinct differences in the mechanism(s) by which oligolysines and PLL provoke DNA condensation to nanoparticles... - Long-term maintenance of patterns of hippocampal pyramidal cells on substrates of polyethylene glycol and microstamped polylysineD W Branch
Biophysics Program, Beckman Institute, Urbana, IL, USA
IEEE Trans Biomed Eng 47:290-300. 2000..We conclude that these materials, especially PEG, are adequate for the maintenance of long-term patterned cultures of neurons. We believe that this is the first report of high-quality long-term patterning of cultured neurons... - Oral uptake and translocation of a polylysine dendrimer with a lipid surfaceA T Florence
Centre for Drug Delivery Research, School of Pharmacy, University of London, 29 39, Brunswick Square, London, UK
J Control Release 65:253-9. 2000..These levels of uptake and translocation are lower than those exhibited by polystyrene particles in the range from 50 to 3000 nm. This might suggest that there is an optimum size for nanoparticulate uptake by the gut... - Glycerol and polylysine synergize in their ability to rupture vesicular membranes: a mechanism for increased transferrin-polylysine-mediated gene transferW Zauner
Institute of Biochemistry, University of Vienna, Austria
Exp Cell Res 232:137-45. 1997The presence of about 1.2 M glycerol during transfection with DNA/transferrin-polylysine and DNA/polylysine complexes dramatically increases transgene expression in a variety of cell types, provided that the complexes have an excess of .. - Dehydration-induced conformational changes of poly-L-lysine as influenced by drying rate and carbohydratesW F Wolkers
Department of Biomolecular Sciences, Laboratory of Plant Physiology, Wageningen Agricultural University, Netherlands
Biochim Biophys Acta 1425:127-36. 1998..We conclude that either immobilization by fast air-drying or sufficient interaction with a protectant through hydrogen bonding (slow drying) plays the leading role in the preservation of the aqueous protein structure... - Efficient gene transfer by histidylated polylysine/pDNA complexesP Midoux
Centre de Biophysique Moleculaire, Glycobiologie CNRS UPR4301 and University of Orléans, rue Charles Sadron, F 45071 Orléans Cedex 02, France
Bioconjug Chem 10:406-11. 1999Plasmid/polylysine complexes, which are used to transfect mammalian cells, increase the uptake of DNA, but plasmid molecules are sequestered into vesicles where they cannot escape to reach the nuclear machinery... - Polylysine-coated mica can be used to observe systematic changes in the supercoiled DNA conformation by scanning force microscopy in solutionMalte Bussiek
German Cancer Research Center, Department of Biophysics of Macromolecules, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany
Nucleic Acids Res 31:e137. 2003The conformations of supercoiled (sc) DNA and linear DNA bound to polylysine (PL)-coated mica were investigated by scanning force microscopy (SFM) in solution... - Intracellular processing and stability of DNA complexed with histidylated polylysine conjugatesChristine Goncalves
Centre de Biophysique Moleculaire, CNRS UPR4301, rue Charles Sadron, 45071 Orleans Cedex 02, France
J Gene Med 4:271-81. 2002..The present work has been undertaken in two steps to study the uptake and the intracellular processing of pDNA, which are still poorly understood in the polyfection pathway... - Laterally stabilized complexes of DNA with linear reducible polycations: strategy for triggered intracellular activation of DNA delivery vectorsDavid Oupicky
CRC Institute for Cancer Studies, University of Birmingham, Birmingham B15 2TT, United Kingdom
J Am Chem Soc 124:8-9. 2002.... - Exploring polyethylenimine-mediated DNA transfection and the proton sponge hypothesisAkin Akinc
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
J Gene Med 7:657-63. 2005..Chloroquine, which buffers lysosomes, enhanced the transfection efficiency of N-quaternized PEIs and polylysine by 2-3-fold. In contrast, chloroquine did not improve the transfection efficiency of PEI... - Polylysine and polyornithine gene transfer complexes: a study of complex stability and cellular uptake as a basis for their differential in-vitro transfection efficiencyE Ramsay
Welsh School of Pharmacy, Cardiff University, UK
J Drug Target 10:1-9. 2002..plasmid DNA (pDNA) have demonstrated superior transfection efficiency (up to x 10-fold) compared to equivalent polylysine-based systems in-vitro. The mechanism(s) underlying this observation remains to be elucidated... - Polylysine-coated diamond nanocrystals for MALDI-TOF mass analysis of DNA oligonucleotidesXianglei Kong
Institute of Atomic and Molecular Sciences, Academia Sinica, P.O. Box 23-166, Taipei, Taiwan 106
Anal Chem 77:4273-7. 2005..No preseparation of PL or diamond nanocrystals is required for subsequent MALDI-TOF mass analysis... - Au-nanoparticle nanowires based on DNA and polylysine templatesFernando Patolsky
Institute of Chemistry The Hebrew University of Jerusalem, Jerusalem 91904, Israel
Angew Chem Int Ed Engl 41:2323-7. 2002 - Evaluations of unformulated and formulated dendrimer-based microbicide candidates in mouse and guinea pig models of genital herpesD I Bernstein
Division of Infectious Diseases, Children s Hospital Medical Center, Cincinnati, Ohio 45229, USA
Antimicrob Agents Chemother 47:3784-8. 2003..From these studies a lead compound and formulation (2V of SPL7013) was chosen for ongoing evaluations in primate models of simian immunodeficiency virus and Chlamydia trachomatis infection... - In vivo gene delivery via portal vein and bile duct to individual lobes of the rat liver using a polylysine-based nonviral DNA vector in combination with chloroquineX Zhang
Department of Clinical Sciences, Institute of Liver Studies, Guy's, King's and St. Thomas' School of Medicine, King's College Hospital, Bessemer Road, London SE5 9PJ, UK
Hum Gene Ther 12:2179-90. 2001..The 31-amino-acid peptide (polylysine-molossin) comprises an amino-terminal chain of 16 lysines for electrostatic binding of DNA, and the 15 amino .. - A modified microstamping technique enhances polylysine transfer and neuronal cell patterningJohn C Chang
Department of Electrical and Computer Engineering, Beckman Institute, University of Illinois at Urbana Champaign, 405 N Mathews Ave, Urbana, IL 61801, USA
Biomaterials 24:2863-70. 2003..Therefore, we hypothesized that stamp surface modification (termed here a release layer) can enhance polylysine transfer and benefit cell growth on microstamped substrates... - Cell growth and detachment from protein-coated PNIPAAm-based copolymersMartin T Moran
National Centre for Biomedical Engineering Sciences, National University of Ireland, Galway, Ireland
J Biomed Mater Res A 81:870-6. 2007..The results demonstrate a versatile method for the cultivation of cells while eliminating the need for the use of digestive enzymes such as trypsin... - In vitro and in vivo gene transfer with poly(amino acid) vesiclesMaureen D Brown
Department of Pharmaceutical Sciences, University of Strathclyde, Strachclyde Inst. For Bio. Scis, 22 Taylor Street G4 0NR, Glasgow, UK
J Control Release 93:193-211. 2003..It is possible that this approach may be extended to other polyamines... - Layer-by-layer-assembled microfiltration membranes for biomolecule immobilization and enzymatic catalysisV Smuleac
Department of Chemical and Materials Engineering and Center of Membrane Sciences, University of Kentucky, Lexington, Kentucky 40506, USA
Langmuir 22:10118-24. 2006..Besides simplicity and versatility, the ease of enzyme regeneration constitutes an additional benefit of this approach... - Controlled complexation of plasmid DNA with cationic polymers: effect of surfactant on the complexation and stability of the complexesMarjukka Ikonen
Helsinki University of Technology, Laboratory of Physical Chemistry and Electrochemistry, PO Box 6100, FI 02015 TKK, Finland
Colloids Surf B Biointerfaces 66:77-83. 2008..This study elucidates the role of surfactant in controlling the size of the PEI/pDNA complex and reveals the differences of the two polymers as complexing agents... - Polyion complex micelles from plasmid DNA and poly(ethylene glycol)-poly(L-lysine) block copolymer as serum-tolerable polyplex system: physicochemical properties of micelles relevant to gene transfection efficiencyKeiji Itaka
Department of Materials Science and Engineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
Biomaterials 24:4495-506. 2003..A virus-comparable size (approximately 100 nm) with a serum-tolerable property of the PIC micelles indeed suggests their promising feasibility as non-viral gene-vector systems used for clinical gene therapy... - Chloride accumulation and swelling in endosomes enhances DNA transfer by polyamine-DNA polyplexesN D Sonawane
Departments of Medicine and Physiology, Cardiovascular Research Institute, University of California, San Francisco, California 94143, USA
J Biol Chem 278:44826-31. 2003..endosomal Cl- concentration, pH, and volume after internalization of polyplexes composed of plasmid DNA and polylysine (POL), a non-buffering polyamine, or the strongly buffering polyamines polyethylenimine (PEI) or polyamidoamine (.. - Combined use of polycationic peptide and biodegradable macromolecular polymer as a novel gene delivery system: a preliminary studyYu Nie
Key Laboratory of Drug Targeting and Novel Drug Delivery Systems, West China School of Pharmacy, Sichuan University, Chengdu, Sichuan, PR China
Drug Deliv 13:441-6. 2006Plasmid(p) DNA was condensed by polycationic peptide polylysine (PLL) to be a core and then encapsulated in biodegradable monomethoxy (polyethyleneglycol)-poly(lactide-co-glycolide)-monomethoxy (poly-ethylene glycol) (PELGE) to form core-.. - Use of PLL-g-PEG in micro-fluidic devices for localizing selective and specific protein bindingRodolphe Marie
Division of Solid State Physics, Lund University, P.O. Box 118, SE-221 00, Lund, Sweden
Langmuir 22:10103-8. 2006..Equally good, or almost as good, results were obtained for oxidized SU-8, Topas, and poly(methyl metacrylate) (PMMA), demonstrating the generic potential of PLL-g-PEG for surface modification in various micro-fluidic applications... - Surface modification of PLGA microspheresM Muller
Laboratory for Surface Science and Technology, Department of Materials, Swiss Federal Institute of Technology, ETH Zurich, Switzerland
J Biomed Mater Res A 66:55-61. 2003..Low protein-binding, functionalizable microspheres provide a fundamental basis for the design of drug delivery and biosensor systems... - A comparative study of chemical derivatisation methods for spatially differentiated cell adhesion on 2-dimensional microfabricated polymeric matricesFrancesca Bianchi
Interdepartmental Research Centre E. Piaggio, University of Pisa, Via Diotisalvi 2, 56216 Pisa, Italy
J Biomater Sci Polym Ed 14:1077-96. 2003..PEG) derivatives were employed to render the surface underlying the scaffold anti-adhesive whilst polylysine was covalently coupled to the surface of the polymer matrix to enhance cell adhesion... - Structural characterization of self-assembled polypeptide films on titanium and glass surfaces by atomic force microscopyIstván Pelsöczi
Department of Dentistry and Oral Surgery, University of Szeged, Tisza Lajos krt 64, H 6720 Szeged, Hungary
Biomacromolecules 6:3345-50. 2005..Both surfaces displayed a rehydration capability, the titanium surface exhibiting a better stability in time. The high roughness values observed at acidic or basic pH are related to the degree of ionization of PGA and PLL... - Microcontact printing of novel co-polymers in combination with proteins for cell-biological applicationsGabor Csucs
BioMicroMetricsGroup, BMMG, ETH Zurich, Wagistrasse 4, Schlieren CH 8952, Switzerland
Biomaterials 24:1713-20. 2003.... - Freeze-dried formulations for in vivo gene delivery of PEGylated polyplex micelles with disulfide crosslinked cores to the liverKanjiro Miyata
Department of Materials Science and Engineering, Graduate School of Engineering, The University of Tokyo, Bunkyo-ku, Japan
J Control Release 109:15-23. 2005..This achievement is certainly useful for pharmaceutical applications and exhibits many advantages, including easy concentration adjustments of dosing samples, long-term storage stability, and large-scale production reproducibility... - The molecular structures of poly(ethylene glycol)-modified nonviral gene delivery polyplexesYan Guo
School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200030, PR China
Mol Pharm 1:477-82. 2004..Individual PEG strands were seen to extend from the compact cores and intertwined with each other to form the protective surface layer... - Correction of the growth defect in dwarf mice with nonautologous microencapsulated myoblasts--an alternate approach to somatic gene therapyA Al-Hendy
Department of Pediatrics, McMaster University, Hamilton, Ontario, Canada
Hum Gene Ther 6:165-75. 1995..It should have wide applications in therapies requiring a systemic continuous supply of recombinant gene products... - Physicochemical model of alginate-poly-L-lysine microcapsules defined at the micrometric/nanometric scale using ATR-FTIR, XPS, and ToF-SIMSSusan K Tam
, , C.P. 6079, Succ. Centre-Ville, Qu, Canada
Biomaterials 26:6950-61. 2005..This physicochemical model of APA microcapsules could explain their immunogenicity and will play an important role in the optimization of the microcapsule design... - Preparation of poly(ethylene glycol)-introduced cationized gelatin as a non-viral gene carrierToshihiro Kushibiki
Department of Biomaterials, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawara-cho Shogoin, Sakyo-ku, Kyoto 606-8507, Japan
J Biomater Sci Polym Ed 16:1447-61. 2005..It is concluded that the PEG-cationized gelatin was a promising non-viral gene carrier to enhance gene expression in vivo... - Novel surface entrapment process for the incorporation of bioactive molecules within preformed alginate fibersQingpu Hou
School of Pharmacy, The University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom
Biomacromolecules 6:734-40. 2005..By this technique, poly(L-lysine) (PLL) coupled with cell adhesion peptide sequence GRGDS (PLL-GRGDS) was entrapped within alginate fibers, and it was demonstrated that the modification promoted the attachment of mouse 3T3 fibroblasts... - Copolymers of poly-L-lysine with serine and tryptophan form stable DNA vectors: implications for receptor-mediated gene transferA G Gómez-Valadés
Unitat de Biofisica, Departament de Ciències Fisiològiques II, IDIBELL Universitat de Barcelona, Feixa Llarga s n, 08907 L Hospitalet, Spain
J Control Release 102:277-91. 2005..Moreover, tryptophan-containing particles are able to mediate specific gene transfer via receptor-mediated endocytosis... - DNA-polycation complexation and polyplex stability in the presence of competing polyanionsSigne Danielsen
Biophysics and Medical Technology, Department of Physics, The Norwegian University of Science and Technology, NTNU, NO-7491 Trondheim, Norway
Biopolymers 77:86-97. 2005..Annealing experiments of plasmid DNA-chitosan complexes showed an increased fraction of rods following temperature treatment, indicating that the rods most likely are the more stable morphology for this system... - Sustained release of PEG-g-chitosan complexed DNA from poly(lactide-co-glycolide)Yang H Yun
Department of Biomedical Engineering, 348 Psychology A Building, State University of New York at Stony Brook, Stony Brook, NY 11794-2580, USA
J Biomater Sci Polym Ed 16:1359-78. 2005..In vivo experiments also show that these microspheres are capable of achieving gene transfer in a rat hind limb muscle model... - Polyelectrolyte multilayer film coating and stability at the surfaces of oral prosthesis base polymers: an in vitro and in vivo studyO Etienne
, , 11, rue Humann, 67085 Strasbourg Cedex, France
J Dent Res 85:44-8. 2006..We also showed that saliva does not degrade the film after 7 days in vitro and after 4 days in vivo. Taken together, our results establish that the layer-by-layer technique is suitable for the coating of oral devices... - Effect of functionalization of multilayered polyelectrolyte films on motoneuron growthConstant Vodouhe
INSERM Unité 595, 11 rue Humann, Strasbourg Cedex 67085, France
Biomaterials 26:545-54. 2005..The viability of motoneurons on polyelectrolyte multilayers was higher compared to polyelectrolyte monolayers. BDNF and Sema3A embedded in the films remained active and thereby create functionalized nanofilms... - Biocompatibility of alginate-poly-L-lysine microcapsules for cell therapyGorka Orive
Laboratory of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of the Basque Country, Vitoria Gasteiz, Spain
Biomaterials 27:3691-700. 2006..Improved knowledge will lead to the production of standardized transplantation-grade biomaterials and biocompatible microcapsules... - Interaction of poly(L-lysine)-g-poly(ethylene glycol) with supported phospholipid bilayersFernanda F Rossetti
Bio Interface Group, Laboratory for Surface Science and Technology, Department of Materials, Swiss Federal Institute of Technology, Zurich, Switzerland
Biophys J 87:1711-21. 2004..Other factors included the ionic strength of the buffer used and the quality of the bilayers, pointing toward the important role defects present in the SPBs play in the decomposition process... - Emulsion strategies in the microencapsulation of cells: pathways to thin coherent membranesA Leung
Department of Chemical Engineering, The University of Queensland, Brisbane, Queensland 4072, Australia
Biotechnol Bioeng 92:45-53. 2005..Optical and fluorescence microscopy are used to assess the morphology and coherence of the calcium alginate/poly-L-ornithine/alginate (APA) capsules produced... - Influence of chitosan structure on the formation and stability of DNA-chitosan polyelectrolyte complexesSabina P Strand
NOBIPOL, Department of Biotechnology, and Biophysics and Medical Technology, The Norwegian University of Science and Technology, NTNU, NO 7491 Trondheim, Norway
Biomacromolecules 6:3357-66. 2005..4. Such pH-dependent dissociation of polyplexes around the physiological pH is highly relevant in gene delivery applications and might be one of the reasons for the high transfection activity of oligomer-based polyplexes observed... - Comparative study and improvement of current cell micro-patterning techniquesJenny Fink
Biologie du cycle cellulaire et de la motilité, Institut Curie, CNRS, UMR144, 26 Rue d Ulm, 75005 Paris, France
Lab Chip 7:672-80. 2007..Finally, we present a new method based on UV crosslinking of adhesion proteins with benzophenone to easily and rapidly produce highly reproducible micropatterns without the use of a microfabricated elastomeric stamp... - Surface-tethered DNA complexes for enhanced gene deliveryTatiana Segura
Department of Chemical Engineering, Northwestern University, 2145 Sheridan Road E156, Evanston, Illinois 60208-3120, USA
Bioconjug Chem 13:621-9. 2002..The cationic polymer polylysine (degree of polymerization equal to 19 or 150) was modified with biotin groups, which was confirmed by mass .. - Targeted gene transfer to liver using protein-DNA complexesCatherine H Wu
Division of Gastroenterology-Hepatology, Department of Medicine, University of Connecticut Health Center, Farmington, CT, USA
Methods Mol Med 69:15-23. 2002 - Novel ternary polyplex of triblock copolymer, pDNA and anionic dendrimer phthalocyanine for photochemical enhancement of transgene expressionA Arnida
Department of Materials Science and Engineering, Graduate School of Engineering, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo, Japan
J Control Release 116:e75-7. 2006 - Nanoparticle-mediated gene delivery: state of the artM Ravi Kumar
Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research, SASNagar, Mohali, India
Expert Opin Biol Ther 4:1213-24. 2004.... - Triggered intracellular activation of disulfide crosslinked polyelectrolyte gene delivery complexes with extended systemic circulation in vivoD Oupicky
CRC Institute for Cancer Studies, University of Birmingham, Birmingham B15 2TA, UK
Gene Ther 8:713-24. 2001..The extended systemic circulation together with triggered intracellular activation makes these complexes a promising system for targeted gene delivery in vivo... - Polymers for DNA deliveryH Eliyahu
Department of Medicinal Chemistry and Natural Products, School of Pharmacy, Jerusalem, Israel
Molecules 10:34-64. 2005..It introduces the most important cationic polymers used for gene delivery. A transition from in vitro to in vivo gene delivery is also presented, with an emphasis on the obstacles to achieve successful transfection in vivo... - Development of liposomal gene targeting for therapeutic intervention or prevention of restenosisEwa Zant-Przeworska
Groningen University Institute for Drug Exploration (GUIDE, Department of Cell Biology and Liposome Research, A. Deusinglaan 1, 9713 AV Groningen, The Netherlands
Cell Mol Biol Lett 7:268-9. 2002 - Inhibition of tumor growth in mice by endostatin derived from abdominal transplanted encapsulated cellsHuaining Teng
Institute of Biochemistry and Cell Biology, Model Organism Research Center, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
Acta Biochim Biophys Sin (Shanghai) 39:278-84. 2007..These results widen the clinical application of the microencapsulated cell endostatin delivery system in cancer treatment... - Palmitic acid substitution on cationic polymers for effective delivery of plasmid DNA to bone marrow stromal cellsVanessa Incani
Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, Alberta, Canada
J Biomed Mater Res A 81:493-504. 2007..We conclude that PA substitution on PLL provides an effective carrier for transfection of primary cells derived from the bone marrow... - Decomposable hollow biopolymer-based capsulesC Schuler
Max Planck Institute of Colloids and Interfaces, D 14424 Potsdam, Germany
Biomacromolecules 2:921-6. 2001..g., in the bloodstream... - Importance of lateral and steric stabilization of polyelectrolyte gene delivery vectors for extended systemic circulationDavid Oupicky
Cancer Research UK Institute for Cancer Studies, University of Birmingham, Birmingham, B15 2TT, UK
Mol Ther 5:463-72. 2002..Multivalent PHPMA coating allows, for the first time, considerably extended circulation time using polyplex systems-a prerequisite for systemic gene delivery... - Evaluation of cellular uptake and gene transfer efficiency of pegylated poly-L-lysine compacted DNA: implications for cancer gene therapyM Walsh
School of Pharmacy, University College Cork, Cork, Ireland
Mol Pharm 3:644-53. 2006..Such mechanistic studies are an essential step to support future rational design of pegylated poly-L-lysine vectors to improve the efficiency of gene delivery... - Continuous release of endostatin from microencapsulated engineered cells for tumor therapyT Joki
Brain Tumor Laboratory, Department of Neurosurgery, Department of Surgery, Brigham Women's Hospital, Harvard Medical School, Boston, MA 02115, USA
Nat Biotechnol 19:35-9. 2001..Encapsulated endostatin-secreting cells are effective for the treatment of human glioblastoma xenografts. Continuous local delivery of endostatin may offer an effective therapeutic approach to the treatment of a variety of tumor types... - Genetic manipulation of sinusoidal endothelial cellsYoshiyuki Takei
Department of Gastroenterology, Juntendo University School of Medicine, Tokyo, Japan
J Gastroenterol Hepatol 22:S68-72. 2007..So, the PLL-g-HA/DNA system permits targeted delivery of exogenous nucleotide agents selectively to the liver SEC, providing a novel strategy for manipulation of SEC functions... - Improvement of the biocompatibility of alginate/poly-L-lysine/alginate microcapsules by the use of epimerized alginate as a coatingAileen King
Department of Medical Cell Biology, Uppsala University, Box 571, Biomedical Centre, SE 751 23 Uppsala, Sweden
J Biomed Mater Res A 64:533-9. 2003..In conclusion, the use of an epimerized coating on alginate-PLL-alginate can improve the biocompatibility of such capsules but still cannot completely eliminate the detrimental effects of PLL on the biocompatibility of the capsules... - Biomaterial-mediated retroviral gene transfer using self-assembled monolayersCharles A Gersbach
Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Emory University, Atlanta, GA 30332, USA
Biomaterials 28:5121-7. 2007..This work is significant to establishing safe and effective gene therapy strategies, developing efficient methods for gene delivery, and supporting recent progress in the field of biomaterial-mediated gene transfer... - Poly(L-lysine)-modified silica nanoparticles for the delivery of antisense oligonucleotidesShi-Guo Zhu
Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha, Hunan 410078, People's Republic of China
Biotechnol Appl Biochem 39:179-87. 2004..These results indicated that PMS-NP was a suitable delivery vector for antisense ODN, although its delivery efficiency decreased in the presence of a serum-containing medium... - Fully degradable hydrophilic polyals for protein modificationAlexander Yurkovetskiy
Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114-2696, USA
Biomacromolecules 6:2648-58. 2005..The data suggests that hydrophilic polyals may be useful in designing protein conjugates with improved properties... - Cell-type-specific adhesion onto polymer surfaces from mixed cell populationsRobin A Quirk
School of Pharmaceutical Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom
Biotechnol Bioeng 81:625-8. 2003..This generic strategy is illustrated by the covalent attachment of a galactose derivative to a polylysine backbone via the amine functionality... - Intelligent biosynthetic nanobiomaterials (IBNs) for hyperthermic gene deliveryTze Haw Howard Chen
Division of Pharmaceutical Sciences, School of Pharmacy, University of Wisconsin Madison, Madison, Wisconsin 53705 2222, USA
Pharm Res 25:683-91. 2008....
Research Grants
- TRANSPORT MECHANISMS IN CORNEAL ENDOTHELIUMJorge Fischbarg; Fiscal Year: 2004..of the coupling ratio after junctional modification by agents including cadmium chloride, protamine, and polylysine. 2) To confirm and characterize novel routes for trans-endothelial membrane movements of ions which would ..
- IN VITRO INITIATION OF BIOLOGICAL CALCIFICATIONAdele Boskey; Fiscal Year: 1991..and vitamin A), b) proteoglycan synthesis, composition and/or properties (vitamin A, xylosides, hyaluronidase, polylysine), and c) synthesis of other extracellular matrix proteins (cAMP)...
- Role of integrins and activin in granulosa cell growthKutluk Oktay; Fiscal Year: 2007..In pursuit of these specific aims, SIGC will be cultured on polylysine, fibronectin, laminin or collagen in the presence or absence of Activin- A...
- Mucosal Vaccines for PlagueDavid Pascual; Fiscal Year: 2006..DNA delivery system, whereby the vaccinating DNA plasmid will be complexed to reovirus protein (1coupled to polylysine and will be administered via intranasal (i.n.) or intratracheal (i.t.) routes...
- EXPERIMENTAL PHOTOIMMUNOTHERAPY OF OVARIAN CANCERTayyaba Hasan; Fiscal Year: 1999..This involves the site-specific attachment of modified polylysine chains bearing chlorin e6 molecules to the F(ab')2 fragment of the anti-ovarian cancer Mab, OC125...
- Alveolar Bone Regeneration by Transcriptional ControlJeffrey Hollinger; Fiscal Year: 2006..will be delivered to bone using an injectable, biodegradable, biocompatible hydrogel of hyaluronate (HA) and polylysine (PL). The overall hypothesis for this application is that controlled, predictable delivery ofp...
- Engineered Outer Membrane Vesicles as DNA Vaccine Delivery VehiclesDavid Putnam; Fiscal Year: 2007..by non- specific endocytosis or phagocytosis, the surface of the OMVs will be engineered to possess sequences of polylysine to create OMVs with positive zeta potentials...
- CONTROLLED RELEASE OF MACROMOLECULESRobert Langer; Fiscal Year: 2007..characterization of DNA delivery polymers such as modified poly(ethylene imines), poly(beta-amino esters), and polylysine graft imidizole acetic acids...
- GENE THERAPY FOR CYSTIC FIBROSIS VIA THE SEC RECEPTORPamela Davis; Fiscal Year: 2003..The molecular conjugates are comprised of a peptide ligand for the SEC-R covalently bound to polylysine. This complex binds to DNA through charge interactions, and the complete conjugate is ultimately condensed under ..
- Upright Light Microscope WorkstationUlrich Hopfer; Fiscal Year: 2006..of receptor trafficking and signaling (renal proximal tubule cells); the intracellular fate of condensed polylysine/DNA complexes used for gene therapy (pulmonary epithelia); spatial regulation of EOF receptor signaling from ..
- PHSCN Therapies to Prevent Prostate Cancer ProgressionDonna Livant; Fiscal Year: 2009..We have recently developed a more potent derivative of PHSCN: the PHSCN-polylysine dendrimer...
- ISLET TRANSPLANTATION-- PROTECTION BY IMMUNOBARRIERSGordon Weir; Fiscal Year: 2004..Alginate microcapsules with and without polylysine coating will be tested for their ability to protect against these different immune mechanisms in mouse ..