polylysine

Summary

Summary: A peptide which is a homopolymer of lysine.

Top Publications

  1. ncbi Exploring polyethylenimine-mediated DNA transfection and the proton sponge hypothesis
    Akin Akinc
    Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    J Gene Med 7:657-63. 2005
  2. ncbi Efficient gene transfer by histidylated polylysine/pDNA complexes
    P Midoux
    Centre de Biophysique Moleculaire, Glycobiologie CNRS UPR4301 and University of Orléans, rue Charles Sadron, F 45071 Orléans Cedex 02, France
    Bioconjug Chem 10:406-11. 1999
  3. pmc Synthetic double-stranded RNA induces innate immune responses similar to a live viral vaccine in humans
    Marina Caskey
    Laboratory of Cellular Physiology and Immunology, The Rockefeller University, New York, NY 10065, USA
    J Exp Med 208:2357-66. 2011
  4. ncbi DNA and polylysine adsorption and multilayer construction onto cationic lipid-coated microbubbles
    Mark A Borden
    Biomedical Engineering Department, University of California, Davis, California 95616, USA
    Langmuir 23:9401-8. 2007
  5. ncbi Poly-L-Lysine induces fibrosis on alginate microcapsules via the induction of cytokines
    B L Strand
    Department of Biotechnology, Norwegian University of Science and Technology, Trondheim
    Cell Transplant 10:263-75. 2001
  6. ncbi Assaying phosphatidylinositol bisphosphate regulation of potassium channels
    Tibor Rohacs
    Department of Physiology and Biophysics, Mount Sinai School of Medicine, New York, New York 10029, USA
    Methods Enzymol 345:71-92. 2002
  7. ncbi Alginate-polylysine-alginate microcapsules: effect of size reduction on capsule properties
    B L Strand
    Department of Biotechnology, Norwegian University of Science and Technology, 7491 Trondheim, Norway
    J Microencapsul 19:615-30. 2002
  8. ncbi Long-term active antimicrobial coatings for surgical sutures based on silver nanoparticles and hyperbranched polylysine
    Chau Hon Ho
    Freiburg Materials Research Center and Institute for Macromolecular Chemistry, Albert Ludwigs Universitaet Freiburg, Stefan Meier Str 21, 79104 Freiburg, Germany
    J Biomater Sci Polym Ed 24:1589-600. 2013
  9. ncbi Axonal outgrowth of hippocampal neurons on micro-scale networks of polylysine-conjugated laminin
    L Kam
    Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180, USA
    Biomaterials 22:1049-54. 2001
  10. pmc Polylysine-induced 2H NMR-observable domains in phosphatidylserine/phosphatidylcholine lipid bilayers
    C M Franzin
    Department of Chemistry, University of Toronto at Mississauga, Mississauga, Ontario L5L 1C6, Canada
    Biophys J 81:3346-62. 2001

Research Grants

  1. TRANSPORT MECHANISMS IN CORNEAL ENDOTHELIUM
    Jorge Fischbarg; Fiscal Year: 2004
  2. PHSCN Therapies to Prevent Prostate Cancer Progression
    DONNA LUCY LIVANT; Fiscal Year: 2010
  3. MECHANISM OF PC12 CELL ADHESION TO COLLAGEN AND LAMININ
    David Turner; Fiscal Year: 1991
  4. Role of integrins and activin in granulosa cell growth
    Kutluk Oktay; Fiscal Year: 2007
  5. Mucosal Vaccines for Plague
    David Pascual; Fiscal Year: 2006
  6. NOVEL NONVIRAL VECTORS FOR GENE TRANSFER
    Thomas Scanlin; Fiscal Year: 2000
  7. ELA COOPERATIVE ONCOGENE PRODUCTS IN HUMAN TUMOR CELLS
    JOHN RUPPERT; Fiscal Year: 1999
  8. PATTERNED NEURONAL NETWORKS
    Bruce Wheeler; Fiscal Year: 1991
  9. EXTRACELLULAR MODULATION OF UROTHELIAL PERMEABILITY
    Simon Lewis; Fiscal Year: 2000
  10. LARGE DELETION ADENOVIRAL VECTORS FOR CYSTIC FIBROSIS
    WANDA O NEAL; Fiscal Year: 1999

Detail Information

Publications255 found, 100 shown here

  1. ncbi Exploring polyethylenimine-mediated DNA transfection and the proton sponge hypothesis
    Akin Akinc
    Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    J Gene Med 7:657-63. 2005
    ..According to the proton sponge hypothesis, the buffering capacity of PEI leads to osmotic swelling and rupture of endosomes, resulting in the release of the vector into the cytoplasm...
  2. ncbi Efficient gene transfer by histidylated polylysine/pDNA complexes
    P Midoux
    Centre de Biophysique Moleculaire, Glycobiologie CNRS UPR4301 and University of Orléans, rue Charles Sadron, F 45071 Orléans Cedex 02, France
    Bioconjug Chem 10:406-11. 1999
    Plasmid/polylysine complexes, which are used to transfect mammalian cells, increase the uptake of DNA, but plasmid molecules are sequestered into vesicles where they cannot escape to reach the nuclear machinery...
  3. pmc Synthetic double-stranded RNA induces innate immune responses similar to a live viral vaccine in humans
    Marina Caskey
    Laboratory of Cellular Physiology and Immunology, The Rockefeller University, New York, NY 10065, USA
    J Exp Med 208:2357-66. 2011
    ..Therefore, a chemically defined PRR agonist like poly ICLC can be a reliable and authentic microbial mimic for inducing innate immune responses in humans...
  4. ncbi DNA and polylysine adsorption and multilayer construction onto cationic lipid-coated microbubbles
    Mark A Borden
    Biomedical Engineering Department, University of California, Davis, California 95616, USA
    Langmuir 23:9401-8. 2007
    ..These results suggest that the surface can be compartmentalized to make multifunctional, high-payload ultrasound contrast agents for targeted gene therapy...
  5. ncbi Poly-L-Lysine induces fibrosis on alginate microcapsules via the induction of cytokines
    B L Strand
    Department of Biotechnology, Norwegian University of Science and Technology, Trondheim
    Cell Transplant 10:263-75. 2001
    ..By combining soluble PLL and alginate both the toxic and TNF-inducing effects of PLL were reduced. The implications of these data are to use alginate microcapsules with low amounts of PLL for transplantation purposes...
  6. ncbi Assaying phosphatidylinositol bisphosphate regulation of potassium channels
    Tibor Rohacs
    Department of Physiology and Biophysics, Mount Sinai School of Medicine, New York, New York 10029, USA
    Methods Enzymol 345:71-92. 2002
  7. ncbi Alginate-polylysine-alginate microcapsules: effect of size reduction on capsule properties
    B L Strand
    Department of Biotechnology, Norwegian University of Science and Technology, 7491 Trondheim, Norway
    J Microencapsul 19:615-30. 2002
    Alginate-polylysine-alginate capsules containing insulin-producing cells have been used as a bio-artificial pancreas in the treatment of diabetes mellitus...
  8. ncbi Long-term active antimicrobial coatings for surgical sutures based on silver nanoparticles and hyperbranched polylysine
    Chau Hon Ho
    Freiburg Materials Research Center and Institute for Macromolecular Chemistry, Albert Ludwigs Universitaet Freiburg, Stefan Meier Str 21, 79104 Freiburg, Germany
    J Biomater Sci Polym Ed 24:1589-600. 2013
    ..To this end, two water-insoluble polymeric nanocontainers based on hyperbranched polylysine (HPL), hydrophobically modified by either using glycidyl hexadecyl ether, or a mixture of stearoyl/palmitoyl ..
  9. ncbi Axonal outgrowth of hippocampal neurons on micro-scale networks of polylysine-conjugated laminin
    L Kam
    Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180, USA
    Biomaterials 22:1049-54. 2001
    Microcontact printing was used to define an interconnected lattice network of polylysine-conjugated laminin, a protein-polypeptide ligate that is an effective promoter of neuron outgrowth on material surfaces...
  10. pmc Polylysine-induced 2H NMR-observable domains in phosphatidylserine/phosphatidylcholine lipid bilayers
    C M Franzin
    Department of Chemistry, University of Toronto at Mississauga, Mississauga, Ontario L5L 1C6, Canada
    Biophys J 81:3346-62. 2001
    ..The intensities and quadrupolar splittings of the two spectral components were consistent with the existence of polylysine-bound domains enriched in POPS, in coexistence with polylysine-free domains depleted in POPS...
  11. ncbi Characterization of biophysical and metabolic properties of cells labeled with superparamagnetic iron oxide nanoparticles and transfection agent for cellular MR imaging
    Ali S Arbab
    Experimental Neuroimaging Section, Laboratory of Diagnostic Radiology Research, National Institutes of Health, 10 Center Dr, Rm B1N256, Bethesda, MD 20892, USA
    Radiology 229:838-46. 2003
    ..To evaluate the effect of using the ferumoxides-poly-l-lysine (PLL) complex for magnetic cell labeling on the long-term viability, function, metabolism, and iron utilization of mammalian cells...
  12. ncbi Effect of human stem cells labeled with ferumoxides-poly-L-lysine on hematologic and biochemical measurements in rats
    Gene T Yocum
    Experimental Neuroimaging Section, Laboratory of Diagnostic Radiology Research, Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA
    Radiology 235:547-52. 2005
    ....
  13. ncbi Clinically applicable labeling of mammalian and stem cells by combining superparamagnetic iron oxides and transfection agents
    Joseph A Frank
    Laboratory of Diagnostic Radiology Research, Clinical Center, National Institutes of Health, Bldg 10, Rm B1N256, 10 Center Dr, MSC 1074, Bethesda, MD 20892 1074, USA
    Radiology 228:480-7. 2003
    ..To label mammalian and stem cells by combining commercially available transfection agents (TAs) with superparamagnetic iron oxide (SPIO) magnetic resonance (MR) imaging contrast agents...
  14. ncbi DNA strand exchange stimulated by spontaneous complex formation with cationic comb-type copolymer
    Won Jong Kim
    Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, and PRESTO, Japan Science and Technology Corporation, Midori, Yokohama 226 8501, Japan
    J Am Chem Soc 124:12676-7. 2002
    ..CCCs stabilize not only matured hybrids but also the nucleation complex to accelerate hybridization...
  15. ncbi Toxicity and dose response of intra-abdominally administered poly-L-alpha-lysine and poly-L-glutamate for postoperative adhesion protection
    K Isaksson
    Department of Surgery, University Hospital of Lund, Lund, Sweden
    Eur Surg Res 44:17-22. 2010
    ..This study aims to investigate the possible toxic effects and to establish a lowest effective antiadhesive dose...
  16. ncbi Poly(L-lysine)-modified iron oxide nanoparticles for stem cell labeling
    Michal Babic
    Institute of Macromolecular Chemistry, v v i, Academy of Sciences of the Czech Republic, Heyrovsky Sq 2, 162 06 Prague 6, Czech Republic
    Bioconjug Chem 19:740-50. 2008
    ..In comparison with Endorem, nanoparticles modified with PLL of an optimum molecular weight demonstrated a higher efficiency of intracellular uptake by MSC cells...
  17. ncbi Thermodynamic properties underlying the alpha-helix-to-beta-sheet transition, aggregation, and amyloidogenesis of polylysine as probed by calorimetry, densimetry, and ultrasound velocimetry
    Vytautas Smirnovas
    J Phys Chem B 109:19043-5. 2005
    In this work, we performed a detailed thermodynamic study of an aggregation-prone polypeptide, polylysine, to gain a deeper insight into the scenario of physicochemical events during its unfolding, aggregation, and amyloidogenesis...
  18. ncbi Prevention of postoperative peritoneal adhesions: effects of lysozyme, polylysine and polyglutamate versus hyaluronic acid
    Laszlo Nehez
    Department of Surgery, Lund University, Sweden
    Scand J Gastroenterol 40:1118-23. 2005
    ..In the present study we hypothesized that a combination of polypeptides with different surface properties, resulting in fine disperse low-soluble complexes, could be of benefit in the prevention of abdominal adhesions...
  19. ncbi Epsilon-polylysine microparticle adjuvant drives cytokine production to Th1 profile
    Hiroaki Wakamoto
    Basic Veterinary Science, Doctoral Course of the United Graduate School of Veterinary Sciences, Gifu University, National University Corporation, 1 1 Yanagido, Gifu shi 501 1193, Japan
    J Vet Med Sci 69:717-23. 2007
    Epsilon-polylysine micro particles (SGEPL) and polyethyleneimine micro particles (SGPEI) were developed by the addition of a hydrophobic group and the immunological characterization of these micro particles and aluminum hydroxide (ALUM) ..
  20. pmc Evaluations of unformulated and formulated dendrimer-based microbicide candidates in mouse and guinea pig models of genital herpes
    D I Bernstein
    Division of Infectious Diseases, Children s Hospital Medical Center, Cincinnati, Ohio 45229, USA
    Antimicrob Agents Chemother 47:3784-8. 2003
    ..From these studies a lead compound and formulation (2V of SPL7013) was chosen for ongoing evaluations in primate models of simian immunodeficiency virus and Chlamydia trachomatis infection...
  21. ncbi Poly(L-lysine)-GRGDS as a biomimetic surface modifier for poly(lactic acid)
    R A Quirk
    School of Pharmaceutical Sciences, The University of Nottingham, University Park, UK
    Biomaterials 22:865-72. 2001
    ..Potentially, this is a flexible method of surface modification that can engineer many different types of tissue engineering scaffolds with a variety of biomolecules, thus allowing initial cell adhesion to be controlled...
  22. ncbi Systemic circulation of poly(L-lysine)/DNA vectors is influenced by polycation molecular weight and type of DNA: differential circulation in mice and rats and the implications for human gene therapy
    C M Ward
    CRC Institute for Cancer Studies, University of Birmingham, United Kingdom
    Blood 97:2221-9. 2001
    ..Analysis using human blood in vitro shows no hemolysis, but both types of complexes fix complement and bind IgG, suggesting that pLL/DNA complexes may be rapidly cleared from the human circulation...
  23. ncbi Novel treatment in peritoneal adhesion prevention: protection by polypeptides
    Laszlo Nehez
    Department of Surgery, Center of Chemistry and Clinical Engineering, Lund University, Sweden
    Scand J Gastroenterol 41:1110-7. 2006
    ..To evaluate a novel antiadhesive polypeptide complex containing a combination of poly-L-glutamate and poly-L-lysine in order to study its effectiveness and mechanisms in the prevention of postoperative abdominal adhesions in mice...
  24. ncbi Coating of negatively charged liposomes by polylysine: drug release study
    Dmitry Volodkin
    Institut National de la Sante et de la Recherche Medicale, Unite 595, Faculte de Chirurgie Dentaire, 11 rue Humann, 67085 Strasbourg Cedex, France
    J Control Release 117:111-20. 2007
    ..Moreover, PLL does not prevent lipid exchange between vesicles induced by temperature change...
  25. ncbi Polylysine-coated diamond nanocrystals for MALDI-TOF mass analysis of DNA oligonucleotides
    Xianglei Kong
    Institute of Atomic and Molecular Sciences, Academia Sinica, P O Box 23 166, Taipei, Taiwan 106
    Anal Chem 77:4273-7. 2005
    ..No preseparation of PL or diamond nanocrystals is required for subsequent MALDI-TOF mass analysis...
  26. ncbi Differently charged polypeptides in the prevention of post-surgical peritoneal adhesions
    Laszlo Nehez
    Department of Surgery, Lund University Hospital, SE 221 85 Lund, Sweden
    Scand J Gastroenterol 42:519-23. 2007
    ....
  27. ncbi Capping methotrexate α-carboxyl groups enhances systemic exposure and retains the cytotoxicity of drug conjugated PEGylated polylysine dendrimers
    Lisa M Kaminskas
    Drug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University, 381 Royal Parade, Parkville, VIC 3052, Australia
    Mol Pharm 8:338-49. 2011
    A generation 5 PEGylated (PEG 1100) polylysine dendrimer, conjugated via a stable amide linker to OtBu protected methotrexate (MTX), was previously shown to have a circulatory half-life of 2 days and to target solid tumors in both rats ..
  28. pmc Both the C-terminal polylysine region and the farnesylation of K-RasB are important for its specific interaction with calmodulin
    Ling Jia Wu
    State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei, China
    PLoS ONE 6:e21929. 2011
    ..However, the mechanism for the interaction of CaM with K-RasB is not well understood...
  29. pmc Structure of modified ε-polylysine micelles and their application in improving cellular antioxidant activity of curcuminoids
    Hailong Yu
    Department of Food Science, Rutgers, The State University of New Jersey, 65 Dudley Road, New Brunswick, New Jersey 08901, USA
    Food Funct 2:373-80. 2011
    The micelle structure of octenyl succinic anhydride modified ε-polylysine (M-EPL), an anti-microbial surfactant prepared from natural peptide ε-polylysine in aqueous solution has been studied using synchrotron small-angle X-ray ..
  30. pmc Differential modulation of the active site environment of human carbonic anhydrase XII by cationic quantum dots and polylysine
    Sumathra Manokaran
    Department of Chemistry, Biochemistry and Molecular Biology, North Dakota State University, Fargo, ND 58105, USA
    Biochim Biophys Acta 1804:1376-84. 2010
    ..site pocket of human carbonic anhydrase XII (hCA XII), both positively charged CdTe quantum dots (Qds(+)) and polylysine electrostatically interact with the enzyme, and such interaction does not influence the catalytic activity of ..
  31. ncbi Understanding the effect of polylysine architecture on DNA binding using molecular dynamics simulations
    Robert M Elder
    Department of Chemical and Biological Engineering, University of Colorado at Boulder, Colorado 80309, United States
    Biomacromolecules 12:3870-9. 2011
    ..These trends agree with experimental results that show higher transfection efficiency for Poly3 and Poly4 grafted oligolysines than for Poly5, Poly2, and linear PLL...
  32. ncbi Phytoglycogen octenyl succinate, an amphiphilic carbohydrate nanoparticle, and epsilon-polylysine to improve lipid oxidative stability of emulsions
    Siqi L Scheffler
    The Department of Food Science, Purdue University, 745 Agriculture Mall Drive, West Lafayette, Indiana 47907 1160, USA
    J Agric Food Chem 58:660-7. 2010
    Phytoglycogen octenyl succinate (PG-OS) and epsilon-polylysine (EPL) were used to form oil-in-water emulsions with enhanced lipid oxidative stability...
  33. ncbi Novel biodegradable powder for preventing postoperative pleural adhesion
    Katsunori Takagi
    Division of Surgical Oncology, Department of Translational Medical Sciences, Nagasaki University Graduate School of Biomedical Sciences, Sakamoto 1 7 1, Nagasaki 852 8501, Japan
    J Surg Res 179:e13-9. 2013
    ..The aim of this study was to investigate the anti-adhesive effects of commercially available anti-adhesive agents and a newly developed powder-type anti-adhesive agent...
  34. ncbi Polylysine induces an antiparallel actin dimer that nucleates filament assembly: crystal structure at 3.5-A resolution
    Michael R Bubb
    Research Service, Malcom Randall Department of Veterans Affairs Medical Center and the University of Florida College of Medicine, Gainesville, Florida 32608, USA
    J Biol Chem 277:20999-1006. 2002
    ..We now show that latrunculin A, a marine natural product that inhibits actin polymerization, arrests polylysine-induced nucleation at the level of an antiparallel dimer, resulting in its accumulation...
  35. ncbi DNA delivery with hyperbranched polylysine: a comparative study with linear and dendritic polylysine
    Zuzana Kadlecova
    Ecole Polytechnique Federale de Lausanne EPFL, Institut des Matériaux et Institut des Sciences et Ingénierie Chimiques, Laboratoire des Polymères, Bâtiment MXD, Station 12, CH 1015 Lausanne, Switzerland
    J Control Release 169:276-88. 2013
    PEI and polylysine are among the most investigated synthetic polymeric carriers for DNA delivery...
  36. ncbi Interaction and transport of poly(L-lysine) dendrigrafts through liposomal and cellular membranes: the role of generation and surface functionalization
    Ioannis Tsogas
    Institute of Physical Chemistry, NCSR Demokritos, 15310 Aghia Paraskevi, Attiki, Greece
    Biomacromolecules 8:3263-70. 2007
    ..The efficiency of dendrigraft internalization was enhanced by the presence of the guanidinium groups, while DGLs were preferentially localized in the nucleus and nuclear membrane, as revealed by fluorescence microscopy...
  37. ncbi The impact of DNA topology on polyplex uptake and transfection efficiency in mammalian cells
    Arjun Dhanoya
    The Advanced Centre for Biochemical Engineering, University College London, Torrington Place, London, UK
    J Biotechnol 155:377-86. 2011
    ..6%. Collectively these findings emphasize the importance of pDNA topology when complexed with PLL for gene delivery with the SC-form being a key pre-requisite...
  38. ncbi Protease-mediated phototoxicity of a polylysine-chlorin(E6) conjugate
    Yongdoo Choi
    Center for Molecular Imaging Research, Massachusetts General Hospital, 149 13th Street, Room 5406, Charlestown, Massachusetts 02129, USA
    ChemMedChem 1:698-701. 2006
  39. ncbi PEG grafted polylysine with fusogenic peptide for gene delivery: high transfection efficiency with low cytotoxicity
    Haeshin Lee
    Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373 1 Kusong Dong, Yusong gu, 305 701, Taejon, South Korea
    J Control Release 79:283-91. 2002
    ..This suggests that maintaining the size of DNA/polymer complexes after KALA coating plays an important role in gene transfection. KALA/DNA/PEG-g-PLL complexes exhibited lower cytotoxicity compared with other polymer/DNA complexes...
  40. pmc Inhibition of epsilon-poly-L-lysine biosynthesis in Streptomycetaceae bacteria by short-chain polyols
    Masanobu Nishikawa
    Research Institute for Biological Sciences Okayama RIBS Okayama, 7549 1 Kibichuo cho, Okayama 716 1241, Japan
    Appl Environ Microbiol 72:2306-12. 2006
    ..Taken together, these results indicate that ePL synthesis is inhibited by polyols via esterification and that ePL elongation occurs via the incorporation of lysine monomers into the carboxyl terminus of ePL...
  41. ncbi Conformation and assembly of polypeptide scaffolds in templating the synthesis of silica: an example of a polylysine macromolecular "switch"
    Siddharth V Patwardhan
    Department of Chemical and Material Engineering, University of Cincinnati, Ohio 45221 0012, USA
    Biomacromolecules 7:491-7. 2006
    ..hexagonal plates was suggested, via circular dichroic spectroscopy (CD), to result from the assembly of helical polylysine molecules. We confirm that the formation of PLL helices is a prerequisite to the hexagonal silica synthesis...
  42. ncbi Biodegradability of synthetic branched polypeptide with poly(L-lysine) backbone
    F Hudecz
    , , Budapest
    Biol Chem Hoppe Seyler 370:1019-26. 1989
    ..The degradation is significantly retarded as compared to that of alpha- or epsilon-polylysine. A mechanism of degradation is suggested based on the experimental results.
  43. ncbi Targeted imaging of activated caspase-3 in the central nervous system by a dual functional nano-device
    Yang Liu
    Key Laboratory of Smart Drug Delivery, Ministry of Education and PLA, Department of Pharmaceutics, School of Pharmacy, Fudan University, 826 Zhangheng Road, Shanghai 201203, PR China
    J Control Release 163:203-10. 2012
    ..Taken together, our nano-device would help to image activated caspase-3 in vivo and hold great promise in early diagnosis of neurodegenerative diseases...
  44. ncbi Chain-length dependence of alpha-helix to beta-sheet transition in polylysine: model of protein aggregation studied by temperature-tuned FTIR spectroscopy
    Wojciech Dzwolak
    High Pressure Research Center, Polish Academy of Sciences, Sokolowska 29 37, 01 142 Warsaw, Poland
    Biopolymers 73:463-9. 2004
    ..Namely, the chains are arranged in an antiparallel manner because of energetically favored antiparallel pre-assembly of dipolar alpha helices...
  45. ncbi Cationic antimicrobial (ε-polylysine)-anionic polysaccharide (pectin) interactions: influence of polymer charge on physical stability and antimicrobial efficacy
    Yuhua Chang
    Department of Food Science, University of Massachusetts, Amherst, Massachusetts 01003, United States
    J Agric Food Chem 60:1837-44. 2012
    The cationic biopolymer ε-polylysine (ε-PL) is a potent food-grade antimicrobial that is highly effective against a range of food pathogens and spoilage organisms...
  46. ncbi Gene transfer into human hepatoma cells by receptor-associated protein/polylysine conjugates
    Tae Gyun Kim
    Department of Pharmacology, National Institute of Toxicological Research, Korea Food and Drug Administration, Seoul 122 704, Republic of Korea
    Bioconjug Chem 15:326-32. 2004
    ..reamplified by using a primer containing a cysteine codon at its carboxyl end to facilitate its conjugation to polylysine (polyK)...
  47. ncbi Kinetically different populations of O-pyromellityl-gramicidin channels induced by poly-L-lysines in lipid bilayers
    A V Krylov
    A N Belozersky Institute of Physico Chemical Biology and Department of Polymer Sciences, School of Chemistry, Moscow State University, Moscow 119899, Russia
    J Membr Biol 189:119-30. 2002
    ..As found in our previous work, the interaction of polylysine with OPg led to the deceleration of the OPg photoinactivation kinetics, i.e...
  48. ncbi Pegylation of a chlorin(e6) polymer conjugate increases tumor targeting of photosensitizer
    M R Hamblin
    Wellman Laboratories of Photomedicine, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Cancer Res 61:7155-62. 2001
    ..Taken together, these results suggest that pegylation of a polymer-photosensitizer conjugate improves tumor-targeting and may increase the efficacy of photodynamic therapy for ovarian cancer...
  49. pmc Novel high-resolution micropatterning for neuron culture using polylysine adsorption on a cell repellant, plasma-polymerized background
    Wesley C Chang
    Department of Ophthalmology and Phsiology, Neuroscience and Bioengineering Programs, University of California, San Francisco, CA, USA
    Langmuir 24:13048-57. 2008
    ..this process exploits the often-overlooked tendency for the surface of this PEO-like material to adsorb polylysine from aqueous solution while remaining nonfouling with respect to other species, such as bovine serum albumin (..
  50. pmc Nuclear entry of hyperbranched polylysine nanoparticles into cochlear cells
    Weikai Zhang
    Department of Otolaryngology, University of Tampere, Medical School, Finland
    Int J Nanomedicine 6:535-46. 2011
    ..This study aimed to investigate the potential nuclear entry of hyperbranched polylysine nanoparticles (HPNPs) for gene delivery to cochlear targets.
  51. ncbi Improvement of the biocompatibility of alginate/poly-L-lysine/alginate microcapsules by the use of epimerized alginate as a coating
    Aileen King
    Department of Medical Cell Biology, Uppsala University, Box 571, Biomedical Centre, SE 751 23 Uppsala, Sweden
    J Biomed Mater Res A 64:533-9. 2003
    ..In conclusion, the use of an epimerized coating on alginate-PLL-alginate can improve the biocompatibility of such capsules but still cannot completely eliminate the detrimental effects of PLL on the biocompatibility of the capsules...
  52. ncbi Reconfiguring polylysine architectures for controlling polyplex binding and non-viral transfection
    Sangram S Parelkar
    Polymer Science and Engineering Department, University of Massachusetts, 120 Governors Drive, Amherst, MA 01003, USA
    Biomaterials 32:2432-44. 2011
    ..We find that reconfiguring polylysine into short oligolysine grafts, strung from a hydrophobic polymer backbone, gives transfection reagents greatly ..
  53. ncbi Oligonucleotide probes containing polylysine residues for fabrication of DNA chips on various solid surfaces
    Asya Levina
    Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia
    Biotechnol J 2:879-85. 2007
    ..technique for DNA chips preparation on various unmodified surfaces using one type of oligonucleotide derivative, polylysine-oligonucleotide conjugates (PL-oligo)...
  54. ncbi Evaluation on the use of reactive dye-modified polylysine as the biomarker in immunochromatographic test application
    Wei Feng Chang
    Department of Chemical Engineering, National Chung Hsing University, Taichung 402, Taiwan
    Anal Biochem 411:236-40. 2011
    An improved dye immunochromatographic test (DICT) using polylysine (PL) as conjugate spacer loading dye molecules to enhance chromophor color intensity with the potential of a simultaneous multicolored assay has been developed...
  55. pmc The natural antimicrobial peptide subtilosin acts synergistically with glycerol monolaurate, lauric arginate, and ε-poly-L-lysine against bacterial vaginosis-associated pathogens but not human lactobacilli
    Katia Sutyak Noll
    Rutgers, The State University of New Jersey, New Brunswick, New Jersey, USA
    Antimicrob Agents Chemother 56:1756-61. 2012
    ..This is the first report on the effects of subtilosin combined with other natural antimicrobials against G. vaginalis...
  56. ncbi On the kinetics of polyplex endocytic trafficking: implications for gene delivery vector design
    M Laird Forrest
    Department of Chemical and Biomolecular Engineering, University of Illinois, Urbana, Illinois 61801, USA
    Mol Ther 6:57-66. 2002
    ..Additionally, PEI does not appear to buffer endocytic compartments, but requires exposure to an acidic environment for efficient gene delivery...
  57. ncbi Evaluation of dendrimer SPL7013, a lead microbicide candidate against herpes simplex viruses
    Edwin Gong
    Viridae Clinical Sciences Inc, Vancouver, BC, Canada
    Antiviral Res 68:139-46. 2005
    ..The 50% cytotoxic concentrations (CC(50)) in all cases were greater than 10,000 microg/ml. Our data indicate that SPL7013 is a promising candidate for development as a vaginal microbicide and a therapeutic agent...
  58. ncbi Dendritic alpha,epsilon-poly(L-lysine)s as delivery agents for antisense oligonucleotides
    Khee Dong Eom
    Nanormics Inc, Sungbuk Gu, Seoul, 136 130, South Korea
    Pharm Res 24:1581-9. 2007
    ..To evaluate the potential use of dendritic alpha,epsilon-poly(L-lysine)s (DPL) for the efficient cellular delivery of antisense oligonucleotides...
  59. ncbi Changes in the amide I FT-IR bands of poly-L-lysine on spray-drying from alpha-helix, beta-sheet or random coil conformations
    Alexander Mauerer
    Department of Pharmaceutics, Friedrich Alexander University, Erlangen, Germany
    Eur J Pharm Biopharm 62:131-42. 2006
    ..The efficacy of the trehalose could be related to the ability of newly-formed trehalose/PLS intermolecular H-bonds to stabilize the intramolecular H-bonds of the secondary structural elements...
  60. ncbi Safety, tolerability, and pharmacokinetics of SPL7013 gel (VivaGel): a dose ranging, phase I study
    John O'Loughlin
    Royal Adelaide Hospital, Adelaide, Australia
    Sex Transm Dis 37:100-4. 2010
    ..To evaluate safety, tolerability and systemic pharmacokinetics of escalating doses of SPL7013 Gel in healthy women...
  61. ncbi Microbial synthesis of poly(epsilon-lysine) and its various applications
    Ing Lung Shih
    Department of Environmental Engineering, Da Yeh University, 112 Shan Jiau Road, Chang Hwa 51505, Taiwan
    Bioresour Technol 97:1148-59. 2006
    ....
  62. ncbi Preparation of catalytically active, covalent α-polylysine-enzyme conjugates via UV/vis-quantifiable bis-aryl hydrazone bond formation
    Andrea Grotzky
    Department of Materials, ETH Zürich, Wolfgang Pauli Strasse 10, CH 8093 Zürich, Switzerland
    Biomacromolecules 12:134-44. 2011
    ..Thus, not only a mild and efficient preparation and convenient quantification of a conjugate between the polycationic α-polylysine and enzymes could be shown, but also the complete preservation of the enzymatic activity.
  63. ncbi epsilon-Poly-L-lysine: microbial production, biodegradation and application potential
    T Yoshida
    Department of Biomolecular Science, Gifu University, Yanagido 1 1, 501 1193 Gifu, Japan
    Appl Microbiol Biotechnol 62:21-6. 2003
    ..epsilon-PL producers commonly possess membrane-bound epsilon-PL-degrading aminopeptidase, which might play a role in self-protection...
  64. ncbi Determination of dendrigraft poly-L-lysine diffusion coefficients by taylor dispersion analysis
    Herve Cottet
    Institut des Biomolecules Max Mousseron IBMM, UMR 5247 CNRS Universite de Montpellier 1 Universite de Montpellier 2, place Eugène Bataillon CC 017, 34095, Montpellier Cedex 5, France
    Biomacromolecules 8:3235-43. 2007
    ....
  65. pmc Enhancement of human immunodeficiency virus type 1 replication is not intrinsic to all polyanion-based microbicides
    Secondo Sonza
    Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Victoria 3004, Australia
    Antimicrob Agents Chemother 53:3565-8. 2009
    ..The enhancement effect is likely to be a function of the assay conditions and is not an intrinsic property of these polyanions...
  66. pmc VivaGel (SPL7013 Gel): a candidate dendrimer--microbicide for the prevention of HIV and HSV infection
    Richard Rupp
    Sealy Center for Vaccine Development and Department of Pediatrics, University of Texas Medical Branch, Galveston, Texas 77555 1119, USA
    Int J Nanomedicine 2:561-6. 2007
    ..VivaGel appears to be well tolerated in both animals and humans. This review summarizes the studies of VivaGel and its active ingredient, SPL7013...
  67. ncbi PEGylation of polylysine dendrimers improves absorption and lymphatic targeting following SC administration in rats
    Lisa M Kaminskas
    Drug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University Parkville Campus 381 Royal Pde, Parkville, VIC 3052, Australia
    J Control Release 140:108-16. 2009
    b>Polylysine dendrimers have potential as highly flexible, biodegradable nanoparticular carriers that may also promote lymphatic transport...
  68. pmc A randomized controlled trial of the safety of candidate microbicide SPL7013 gel when applied to the penis
    Marcus Y Chen
    Melbourne Sexual Health Centre, The Alfred Hospital, and School of Population Health, University of Melbourne, Melbourne, Australia
    J Acquir Immune Defic Syndr 50:375-80. 2009
    ..To determine the safety of the candidate vaginal microbicide SPL7013 gel (VivaGel) when applied to the penis...
  69. ncbi Potential novel drug carriers for inner ear treatment: hyperbranched polylysine and lipid nanocapsules
    Verena Scheper
    Department of Otolaryngology, Medical University Hannover, Carl Neuberg Str 1, 30625 Hannover, Germany
    Nanomedicine (Lond) 4:623-35. 2009
    Treatment of sensorineural hearing loss could be advanced using novel drug carriers such as hyperbranched polylysine (HBPL) or lipid nanocapsules (LNCs)...
  70. ncbi A modified microstamping technique enhances polylysine transfer and neuronal cell patterning
    John C Chang
    Department of Electrical and Computer Engineering, Beckman Institute, University of Illinois at Urbana Champaign, 405 N Mathews Ave, Urbana, IL 61801, USA
    Biomaterials 24:2863-70. 2003
    ..Therefore, we hypothesized that stamp surface modification (termed here a release layer) can enhance polylysine transfer and benefit cell growth on microstamped substrates...
  71. ncbi Synthesis, characterization and transfection of a novel folate-targeted multipolymeric nanoparticles for gene delivery
    Yu Nie
    National Engineering Research Center in Biomaterials, Sichuan University, No 29, Wangjiang Road, Chengdu, Sichuan, 610041, People s Republic of China
    J Mater Sci Mater Med 20:1849-57. 2009
    ..Fluorescein sodium and polylysine-condensed DNA (PD) were encapsulated in both PELGA nanoparticles (PELGA-NPs) and folate modified nanoparticles (..
  72. pmc A multi-institution phase II study of poly-ICLC and radiotherapy with concurrent and adjuvant temozolomide in adults with newly diagnosed glioblastoma
    Myrna R Rosenfeld
    Department of Neurology, 3 W Gates, University of Pennsylvania, 3400 Spruce St, Philadelphia, PA 19104, USA
    Neuro Oncol 12:1071-7. 2010
    ..Survival data at 12 and 18 months suggest that this may improve the efficacy of chemoradiation and adjuvant TMZ in this patient population...
  73. ncbi Polylysine and polyornithine gene transfer complexes: a study of complex stability and cellular uptake as a basis for their differential in-vitro transfection efficiency
    E Ramsay
    Welsh School of Pharmacy, Cardiff University, UK
    J Drug Target 10:1-9. 2002
    ..plasmid DNA (pDNA) have demonstrated superior transfection efficiency (up to x 10-fold) compared to equivalent polylysine-based systems in-vitro. The mechanism(s) underlying this observation remains to be elucidated...
  74. ncbi Physicochemical properties and antimicrobial efficacy of electrostatic complexes based on cationic ε-polylysine and anionic pectin
    Yuhua Chang
    Department of Food Science, University of Massachusetts, Amherst, Massachusetts 01003, USA
    J Agric Food Chem 59:6776-82. 2011
    b>Polylysine (ε-PL) is a food-grade cationic antimicrobial that is highly effective against a wide range of food pathogens and spoilage organisms...
  75. ncbi Characterization of interpolyelectrolyte complexes between double-stranded DNA and polylysine comb-type copolymers having hydrophilic side chains
    A Maruyama
    Department of Biomolecular Engineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori, Yokohama, Japan
    Bioconjug Chem 9:292-9. 1998
    ....
  76. pmc Polylysine-coated mica can be used to observe systematic changes in the supercoiled DNA conformation by scanning force microscopy in solution
    Malte Bussiek
    German Cancer Research Center, Department of Biophysics of Macromolecules, Im Neuenheimer Feld 580, D 69120 Heidelberg, Germany
    Nucleic Acids Res 31:e137. 2003
    The conformations of supercoiled (sc) DNA and linear DNA bound to polylysine (PL)-coated mica were investigated by scanning force microscopy (SFM) in solution...
  77. ncbi Polylysine-modified PEG-based hydrogels to enhance the neuro-electrode interface
    Shreyas S Rao
    William G Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 W 19th Avenue, Columbus, OH 43210, USA
    J Biomater Sci Polym Ed 22:611-25. 2011
    ..In particular, polylysine-modified PEG-based hydrogels were synthesized, characterized and shown to promote neural adhesion using a PC12 ..
  78. pmc Mechanistic differences in DNA nanoparticle formation in the presence of oligolysines and poly-L-lysine
    Irina Nayvelt
    Department of Medicine, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, New Brunswick, New Jersey 08903, USA
    Biomacromolecules 8:477-84. 2007
    ..The DNA melting temperature increased with oligolysine concentration. These results indicate distinct differences in the mechanism(s) by which oligolysines and PLL provoke DNA condensation to nanoparticles...
  79. ncbi Polylysine-functionalised thermoresponsive chitosan hydrogel for neural tissue engineering
    K E Crompton
    School of Physics, Department of Materials Engineering, Monash University, Wellington Rd, Clayton, VIC 3800, Australia
    Biomaterials 28:441-9. 2007
    ..The results show that thermally responsive chitosan/GP hydrogels provide a suitable 3D scaffolding environment for neural tissue engineering...
  80. ncbi Spectroscopic investigation of cationic comb-type copolymers/DNA interaction: interpolyelectrolyte complex enhancement synchronized with DNA hybridization
    Yuichi Sato
    Precursory Research for Embryonic Science and Technology PRESTO and Core Research for Evolutional Science and Technology CREST, Japan Science and Technology Agency, 4 1 8 Honcho, Kawaguchi, Saitama 332 0012, Japan
    Langmuir 23:65-9. 2007
    ..The IPEC formation of CCCs with DNA, however, seems highly sensitive to the coil-helix transition of the DNA...
  81. ncbi Intracytoplasmic tagging of cells with ferumoxides and transfection agent for cellular magnetic resonance imaging after cell transplantation: methods and techniques
    Ali S Arbab
    Experimental Neuroimaging Section, Laboratory of Diagnostic Radiology Research, National Institutes of Health, Bethesda, MD 20892, USA
    Transplantation 76:1123-30. 2003
    ..The purpose of this study is to present protocols using SPIO and a polycationic transfection agent for magnetic labeling of cells as a basis for cellular MRI...
  82. pmc C2B polylysine motif of synaptotagmin facilitates a Ca2+-independent stage of synaptic vesicle priming in vivo
    Carin A Loewen
    Molecular, Cellular, and Integrative Neuroscience Program, Department of Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA
    Mol Biol Cell 17:5211-26. 2006
    ..I, a synaptic vesicle protein required for efficient synaptic transmission, contains a highly conserved polylysine motif necessary for function...
  83. pmc Preclinical safety and efficacy assessments of dendrimer-based (SPL7013) microbicide gel formulations in a nonhuman primate model
    D L Patton
    Department of Obstetrics and Gynecology, Box 356460, University of Washington, Seattle, WA 98195 6460, USA
    Antimicrob Agents Chemother 50:1696-700. 2006
    ..However, a single intravaginal application of the 3% (wt/wt) SPL7013 gel did not provide protection from the acquisition of cervical chlamydial infection...
  84. ncbi Cell surface nucleolin serves as receptor for DNA nanoparticles composed of pegylated polylysine and DNA
    Xuguang Chen
    Department of Case Western Reserve University School of Medicine, Cleveland, Ohio 44106, USA
    Mol Ther 16:333-42. 2008
    ..Therefore, the data strongly suggest that cell surface nucleolin serves as a receptor for DNA nanoparticles, and that nucleolin is essential for internalization and/or transport of the nanoparticles from cell surface to the nucleus...
  85. ncbi Long-term maintenance of patterns of hippocampal pyramidal cells on substrates of polyethylene glycol and microstamped polylysine
    D W Branch
    Biophysics Program, Beckman Institute, Urbana, IL, USA
    IEEE Trans Biomed Eng 47:290-300. 2000
    ..We conclude that these materials, especially PEG, are adequate for the maintenance of long-term patterned cultures of neurons. We believe that this is the first report of high-quality long-term patterning of cultured neurons...
  86. ncbi Alginate-PLL microencapsulation: effect on the differentiation of embryonic stem cells into hepatocytes
    Tim Maguire
    Department of Biomedical Engineering, Rutgers University, Piscataway, New Jersey 08854, USA
    Biotechnol Bioeng 93:581-91. 2006
    ..This system may ultimately assist in developing scalable stem cell differentiation strategies...
  87. ncbi Investigation of a new microcapsule membrane combining alginate, chitosan, polyethylene glycol and poly-L-lysine for cell transplantation applications
    T Haque
    Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine, McGill University, Montreal, Quebec, Canada
    Int J Artif Organs 28:631-7. 2005
    ..The study demonstrated that the new membrane can be used for cell entrapment. However, further investigations are needed to assess its potential for long term transplantation and usage in the development of bioartificial organs...
  88. ncbi Comparison of transfection agents in forming complexes with ferumoxides, cell labeling efficiency, and cellular viability
    Ali Syed Arbab
    Laboratory of Diagnostic Radiology Research, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Imaging 3:24-32. 2004
    ..Concentrations of 25 to 50 microg/mL of FEs complexed to TAs in media resulted in sufficient endocytosis of the SPIO into endosomes to detect cells on cellular magnetic resonance imaging...
  89. ncbi The effect of backbone structure on polycation comb-type copolymer/DNA interactions and the molecular assembly of DNA
    Yu Ichi Sato
    PRESTO, Japan Science and Technology Agency, 4 1 8 Honcho, Kawaguchi, Saitama 332 0012, Japan
    Biomaterials 26:703-11. 2005
    ..The study suggested that tailor-made design of "functional polycounterion" is a strategy to engineer molecular assembling of DNA...
  90. ncbi SPL7013 gel as a topical microbicide for prevention of vaginal transmission of SHIV89.6P in macaques
    Yong Hou Jiang
    Washington National Primate Research Center, University of Washington, Seattle, Washington 98195, USA
    AIDS Res Hum Retroviruses 21:207-13. 2005
    ..These results suggest that SPL7013 gel may be a promising anti-HIV microbicide formulation for further evaluation...
  91. ncbi In vitro study of alginate-chitosan microcapsules: an alternative to liver cell transplants for the treatment of liver failure
    Tasima Haque
    Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering and Organs Research Center, Faculty of Medicine, McGill University, 3775 University Street, Montreal, Quebec, Canada H3A 2B4
    Biotechnol Lett 27:317-22. 2005
    ..The AC microcapsule has several advantages compared to more widely used alginate-poly-L-lysine (APA) microcapsules for the application of cell therapy...
  92. ncbi Inhibition of lipases by epsilon-polylysine
    Takahiro Tsujita
    Central Research Laboratory, School of Medicine, Ehime University, Shigenobu, Onsen gun, Ehime 791 0295, Japan
    J Lipid Res 44:2278-86. 2003
    Oral administration of epsilon-polylysine to rats reduced the peak plasma triacylglycerol concentration...
  93. ncbi Double genetic modification of adenovirus fiber with RGD polylysine motifs significantly enhances gene transfer to isolated human pancreatic islets
    Juan L Contreras
    Department of Surgery, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    Transplantation 76:252-61. 2003
    ..Because the arginine-glycine-aspartame (RGD) and polylysine (pK7) motifs have been shown to enhance Ad5 infection through an Ad5 receptor-independent pathway, we ..
  94. ncbi Double modification of adenovirus fiber with RGD and polylysine motifs improves coxsackievirus-adenovirus receptor-independent gene transfer efficiency
    Hongju Wu
    Division of Human Gene Therapy, Department of Medicine, Gene Therapy Center, University of Alabama at Birmingham, Birmingham, AL 35294 2172, USA
    Hum Gene Ther 13:1647-53. 2002
    ..Two motifs, RGD and polylysine (pK7), have been shown to enhance Ad5 infection via CAR-independent pathways when incorporated into fiber ..
  95. ncbi Glycerol and polylysine synergize in their ability to rupture vesicular membranes: a mechanism for increased transferrin-polylysine-mediated gene transfer
    W Zauner
    Institute of Biochemistry, University of Vienna, Austria
    Exp Cell Res 232:137-45. 1997
    The presence of about 1.2 M glycerol during transfection with DNA/transferrin-polylysine and DNA/polylysine complexes dramatically increases transgene expression in a variety of cell types, provided that the complexes have an excess of ..
  96. ncbi Microencapsulation of cells producing therapeutic proteins: optimizing cell growth and secretion
    Anne Mari Rokstad
    Institute of Cancer Research and Molecular Biology, Norwegian University of Science and Technology, Trondheim
    Cell Transplant 11:313-24. 2002
    ....
  97. ncbi Dehydration-induced conformational changes of poly-L-lysine as influenced by drying rate and carbohydrates
    W F Wolkers
    Department of Biomolecular Sciences, Laboratory of Plant Physiology, Wageningen Agricultural University, Netherlands
    Biochim Biophys Acta 1425:127-36. 1998
    ..We conclude that either immobilization by fast air-drying or sufficient interaction with a protectant through hydrogen bonding (slow drying) plays the leading role in the preservation of the aqueous protein structure...
  98. ncbi Use of ADME studies to confirm the safety of epsilon-polylysine as a preservative in food
    Jun Hiraki
    Chisso Corporation, Yokohama, Japan
    Regul Toxicol Pharmacol 37:328-40. 2003
    Epsilon-polylysine is a homopolymer of L-lysine, containing approximately 30 L-lysine subunits, as synthesized in aerobic bacterial fermentation by Streptomyces albulus...
  99. ncbi Oral uptake and translocation of a polylysine dendrimer with a lipid surface
    A T Florence
    Centre for Drug Delivery Research, School of Pharmacy, University of London, 29 39, Brunswick Square, London, UK
    J Control Release 65:253-9. 2000
    ..These levels of uptake and translocation are lower than those exhibited by polystyrene particles in the range from 50 to 3000 nm. This might suggest that there is an optimum size for nanoparticulate uptake by the gut...
  100. ncbi Intracellular processing and stability of DNA complexed with histidylated polylysine conjugates
    Christine Goncalves
    Centre de Biophysique Moleculaire, CNRS UPR4301, rue Charles Sadron, 45071 Orleans Cedex 02, France
    J Gene Med 4:271-81. 2002
    ..The present work has been undertaken in two steps to study the uptake and the intracellular processing of pDNA, which are still poorly understood in the polyfection pathway...
  101. ncbi Laterally stabilized complexes of DNA with linear reducible polycations: strategy for triggered intracellular activation of DNA delivery vectors
    David Oupicky
    CRC Institute for Cancer Studies, University of Birmingham, Birmingham B15 2TT, United Kingdom
    J Am Chem Soc 124:8-9. 2002
    ....

Research Grants62

  1. TRANSPORT MECHANISMS IN CORNEAL ENDOTHELIUM
    Jorge Fischbarg; Fiscal Year: 2004
    ..of the coupling ratio after junctional modification by agents including cadmium chloride, protamine, and polylysine. 2) To confirm and characterize novel routes for trans-endothelial membrane movements of ions which would ..
  2. PHSCN Therapies to Prevent Prostate Cancer Progression
    DONNA LUCY LIVANT; Fiscal Year: 2010
    ..We have recently developed a more potent derivative of PHSCN: the PHSCN-polylysine dendrimer...
  3. MECHANISM OF PC12 CELL ADHESION TO COLLAGEN AND LAMININ
    David Turner; Fiscal Year: 1991
    ..is inhibited at low temperature or in the presence of azide, whereas passive attachment to substrata coated with polylysine or wheat germ agglutinin occurs in the absence of divalent cations, at low temperature, and in the presence of ..
  4. Role of integrins and activin in granulosa cell growth
    Kutluk Oktay; Fiscal Year: 2007
    ..In pursuit of these specific aims, SIGC will be cultured on polylysine, fibronectin, laminin or collagen in the presence or absence of Activin- A...
  5. Mucosal Vaccines for Plague
    David Pascual; Fiscal Year: 2006
    ..DNA delivery system, whereby the vaccinating DNA plasmid will be complexed to reovirus protein (1coupled to polylysine and will be administered via intranasal (i.n.) or intratracheal (i.t.) routes...
  6. NOVEL NONVIRAL VECTORS FOR GENE TRANSFER
    Thomas Scanlin; Fiscal Year: 2000
    ..Lactosylated polylysine, with enhancing agents, gives 90 percent efficiency of transfection in CF cells, similar to that achieved by ..
  7. ELA COOPERATIVE ONCOGENE PRODUCTS IN HUMAN TUMOR CELLS
    JOHN RUPPERT; Fiscal Year: 1999
    ..In this proposal the investigators describe a method for efficient, stable transfection that uses adenovirus-polylysine conjugates as a transfection reagent...
  8. PATTERNED NEURONAL NETWORKS
    Bruce Wheeler; Fiscal Year: 1991
    ..Two array/patterning systems will be investigated: 1. polyimide insulation with a neural substrate of polylysine selectively ablated by laser; 2...
  9. EXTRACELLULAR MODULATION OF UROTHELIAL PERMEABILITY
    Simon Lewis; Fiscal Year: 2000
    ..studies have established that cationic proteins (proteins with a net positive charge such as protamine and polylysine) can compromise the barrier function of the mammalian urinary bladder...
  10. LARGE DELETION ADENOVIRAL VECTORS FOR CYSTIC FIBROSIS
    WANDA O NEAL; Fiscal Year: 1999
    ..In addition, helper virus with a fiber gene modified to contain polylysine residues will be made and will be used to propagate the vectors...
  11. POLYCHELATING AGENT INCORPORATION LIPOSOMES & MICELLES
    John Babich; Fiscal Year: 1999
    ..A new molecule represents low-molecular- weight DTPA-polylysine linked via its N-terminus to a lipid anchor, NGPE-PE (DTPA-PLL-PE)...
  12. CELL-POLYMER IMPLANT USING A RODENT BETA-CELL LINE
    Shelley Weinstock; Fiscal Year: 1993
    ..Polymers may be coated with collagen, gelatin, fibronectin, laminin, or polylysine to enhance adhesion...
  13. Alveolar Bone Regeneration by Transcriptional Control
    Jeffrey Hollinger; Fiscal Year: 2006
    ..will be delivered to bone using an injectable, biodegradable, biocompatible hydrogel of hyaluronate (HA) and polylysine (PL). The overall hypothesis for this application is that controlled, predictable delivery ofp...
  14. RBC MEMBRANE ADHESION AND DEFORMATION ENERGIES
    Evan Evans; Fiscal Year: 1980
    ..mediated by a variety of agents: fibrinogen, dextran, small charged liposomes (simulating "virus-like" bodies), polylysine, and lectins (e.g., conconavalin A)...
  15. RBC MEMBRANE MATERIAL CHARACTERISTICS
    Evan Evans; Fiscal Year: 1980
    ..mediated by a variety of agents: fibrinogen, dextran, small charged liposomes (simulating "virus-like" bodies), polylysine, and lectins (e.g., conconavalin A)...
  16. Engineered Outer Membrane Vesicles as DNA Vaccine Delivery Vehicles
    David Putnam; Fiscal Year: 2007
    ..by non- specific endocytosis or phagocytosis, the surface of the OMVs will be engineered to possess sequences of polylysine to create OMVs with positive zeta potentials...
  17. CONTROLLED RELEASE OF MACROMOLECULES
    Robert Langer; Fiscal Year: 2007
    ..characterization of DNA delivery polymers such as modified poly(ethylene imines), poly(beta-amino esters), and polylysine graft imidizole acetic acids...
  18. HIGH-THROUGHPUT MICROARRAY-BASED MICRODIALYSIS (FAST-MD) WITH PARALLEL MEASUREMEN
    Lili Kudo; Fiscal Year: 2009
    ..existing microdialysis approach, samples will be continuously deposited using a robotic arm on the surface of a polylysine coated glass slide that creates a microarray...
  19. REGULATION OF CORONARY ARTERIAL SMOOTH MUSCLE
    JOSEPH DISALVO; Fiscal Year: 1990
    ..and an appropriate concentration of cationic effector, such as naturally occurring histone-H1 or synthetic polylysine, alters substrate specificity such that dephosphorylation of myosin is markedly enhanced, whereas ..
  20. COUNTER ION CONDENSATION AND THE ELECTROSTATIC POTENTIAL
    GERALD MANNING; Fiscal Year: 2001
    ..the aims of understanding experimentally observed formation of clusters of identically changed polyions, such as polylysine, DNA and synthetic charged polymers; and to apply the potential in the description of the elasticity driven ..
  21. TARGETING TO NORMAL HEPATOCYTES
    George Wu; Fiscal Year: 1990
    ..of targeted rescue: 1) increased delivery of rescue agents to normal hepatocytes using asialoglycoprotein-polylysine carrier molecules to increase the amount of antagonists capable of being coupled to the carrier molecule 2) ..
  22. Upright Light Microscope Workstation
    Ulrich Hopfer; Fiscal Year: 2006
    ..of receptor trafficking and signaling (renal proximal tubule cells); the intracellular fate of condensed polylysine/DNA complexes used for gene therapy (pulmonary epithelia); spatial regulation of EOF receptor signaling from ..
  23. Mutational Analysis of Synaptotagmin Function In Vivo
    NOREEN REIST; Fiscal Year: 2007
    ..4) Do the C2A and C2B polylysine motifs mediate independent functions? By mutating both motifs simultaneously, we will determine whether these ..
  24. MODULATION OF C-MYC EXPRESSION BY TRIPLEX FORMATION
    Donald Miller; Fiscal Year: 2000
    ..4. To develop adenovirus/polylysine and liposome systems to enhance nuclear delivery of c-myc targeted oligonucleotide in animal systems. 5...
  25. Macrophage Targeted Photodynamic Therapy
    MICHAEL HAMBLIN; Fiscal Year: 2004
    ....
  26. Ultraviolet-C Therapy for Onychomycosis
    MICHAEL HAMBLIN; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  27. Assessment of Lung function in Mice
    Jason Bates; Fiscal Year: 2009
    ....
  28. Vaccination With Peptide-loaded alphaDC1s For Malignant Gliomas
    Hideho Okada; Fiscal Year: 2008
    ..Information from this pilot study will establish a basis to design subsequent trials with patient populations in which more significant clinical benefit might be realized. ..
  29. MECHANICS OF ACUTE LUNG INJURY
    Jason Bates; Fiscal Year: 2009
    ..The results of our studies are expected to lead to the design of physiologically-based lung recruitment strategies that can be tailored to the individual patient with ALI. This will set the stage for future clinical trials. ..
  30. Bioengineering A Bioartificial Pancreas
    Emmanuel C Opara; Fiscal Year: 2010
    ..The purpose of this project is to develop a viable strategy to overcome these two barriers and make islet transplantation a more appealing and widely used treatment option for diabetic patients. ..
  31. Polymer Carrier for Ischemia-Inducible Gene Therapy
    David Bull; Fiscal Year: 2007
    ..abstract_text> ..
  32. Primate Models of Autism
    MELISSA DAWN contact BAUMAN; Fiscal Year: 2010
    ....
  33. Cytomegalovirus in the Brain
    Anthony N van den Pol; Fiscal Year: 2010
    ..These experiments will help us understand the mechanisms associated with CMV-induced neurological dysfunction, and how to combat the virus within the brain with minimal complications. ..
  34. Hypocretin Neurons
    Anthony N van den Pol; Fiscal Year: 2010
    ..Understanding the role of the endogenous opioid in hypocretin cells should also facilitate the understanding and treatment of narcolepsy. ..
  35. Photodynamic Therapy for Endodontic Disinfection
    NIKOLAOS SOUKOS; Fiscal Year: 2009
    ..Photodynamic therapy may be an adjunctive procedure to kill residual root canal bacteria after their sensitization with a photoactive drug and their subsequent exposure to light. ..
  36. NABTT-Consortium Therapeutic Studies of CNS Malignancies
    Stuart Grossman; Fiscal Year: 2008
    ..abstract_text> ..
  37. Transport of TNFalpha across the BBB
    Weihong Pan; Fiscal Year: 2008
    ..These transporters could be novel drug targets and therefore provide promising therapeutic potential. [unreadable] [unreadable]..
  38. Natural History of CIN2 in Adolescents
    Anna Barbara Moscicki; Fiscal Year: 2007
    ..S. and specifically assess how best to modify cytology-based screening, utilize HPV diagnostics, and tailor aoDrooriate follow-uo strategies for adolescents and vouna women diaanosed with CIN 2 ..
  39. Predictors of HIV Risk Behavior Among Men in Argentina
    Alex Carballo Dieguez; Fiscal Year: 2009
    ..This is a fundamental step prior to designing programs that mitigate those factors and result in decreased risk. ..
  40. Improved Macaque Safety Model for Topical Microbicides: Post-coital Assessments
    Dorothy Patton; Fiscal Year: 2008
    ..In addition, we will collect parallel assessments, when mating has occurred with a placebo gel (HEC universal placebo) in place. These studies will provide urgently needed data regarding topical microbicide use with coital activity. ..
  41. MR-Guided Delivery and Monitoring of Magnetocapsules Immunoprotecting Islet Cells
    JEFF BULTE; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  42. Transcription Factor Reporter Technology
    Alexei Bogdanov; Fiscal Year: 2006
    ..abstract_text> ..
  43. Controlling DNA Condensation In Vitro
    Nicholas Hud; Fiscal Year: 2005
    ..Dr. Hud's results will have direct implications on the feasibility of using static DNA structures in the development of nonviral gene delivery systems. ..
  44. NATURAL HISTORY OF HPV--INFECTION TO NEOPLASIA
    Anna Barbara Moscicki; Fiscal Year: 2004
    ..The understanding of both local and systemic immune responses to initial and subsequent HPV infections may be key in vaccine or therapeutic developments. ..
  45. MR IMAGING OF MYOCARDIAL VIABILITY AFTER ISCHEMIC INSULT
    Dara Kraitchman; Fiscal Year: 2004
    ..abstract_text> ..
  46. EFFECTS OF ALGINATE COMPOSITION ON ENCAPSULATED CELLS
    Ioannis Constantinidis; Fiscal Year: 2004
    ..Furthermore, the proposed NMR experiments will enhance our understanding of the capabilities of NMR to monitor a tissue-engineered construct in vitro and in vivo. ..
  47. INTEGRIN DEPENDENCE OF MUSCARINIC RECEPTOR SIGNALING
    BARBARA SLACK; Fiscal Year: 2003
    ..abstract_text> ..
  48. Chimeric Yellow Fever/West Nile Virus:Candidate Vaccine
    Rafi Ahmed; Fiscal Year: 2003
    ..abstract_text> ..
  49. LOCAL IMMUNE RESPONSE IN CHLAMYDIA SALPINGITIS
    Dorothy Patton; Fiscal Year: 2003
    ..trachomatis- induced PID. If one chlamydial antigen is the major cause of the DTH response in PID, diagnostic or immunomodulatory efforts can be directed towards that antigen. ..
  50. The Glycocalyx and Endothelial Barrier Regulation
    RANDAL DULL; Fiscal Year: 2005
    ..This K08 will provide the foundation for a research career dedicated to understanding the molecular mechanisms of endothelial barrier regulation. ..
  51. Poly-ICLC Prophylaxis Treatment of Ebola Virus Infection
    Andres Salazar; Fiscal Year: 2005
    ..C) To develop GMP manufacturing methods for Poly-ICLC, in compliance with specific FDA guidance. ..
  52. Age-Dependent Response of Hippocampal Neurons to Stress
    Gregory Brewer; Fiscal Year: 2006
    ..abstract_text> ..
  53. Gene Therapy, Neural Grafts & Parkinson's Disease
    DAVID YUREK; Fiscal Year: 2005
    ..These studies will also determine if combinations of viral vectors expressing different neurotrophic factors can be used to improve the therapeutic effects of dopamine grafts. ..
  54. Topical Microbicide Acceptability
    Alex Carballo Dieguez; Fiscal Year: 2006
    ..abstract_text> ..
  55. Adenoviral vectors to target cells
    Patricia Yotnda; Fiscal Year: 2006
    ..At the conclusion of this study we will have in place an approach that could be implemented to allow truly targeted delivery of therapeutic vectors to disseminated malignancies. ..
  56. Novel PEG-Dendrimeric Contrast Agents for Cancer Imaging
    Yanjun Fu; Fiscal Year: 2006
    ..from 60 possibilities) initiated from a homogeneously-sized polyethyleneglycol (PEG) core having dendritic polylysine "amplifiers" at both termini, to which amplifiers will be bound multiple highly-stable gadolinium (Gd)-based ..
  57. HIGH DENSITY MICROARRAYS WITH QUANTITATIVE QC/QA
    Martin Hessner; Fiscal Year: 2006
    ..Our success will provide an accurate, flexible, lower cost alternative to commercial arrays for laboratories wishing to conduct gene expression studies. ..
  58. ASN Workshop "In Vivo MR Tracking of Stem Cells in the CNS" (R13)
    JEFF BULTE; Fiscal Year: 2005
    ..The outcome of the workshop will be published in Experimental Neurology and available for the scientific community at large. ..
  59. Effects of brain-death on islet recovery and functional*
    John Thompson; Fiscal Year: 2006
    ..Overall, these studies will bring novel information about human islet biology and will provide c potential therapeutic strategy to enhance the therapeutic efficacy of PIT as a cure for diabetes. ..
  60. NOVEL TYROSINASE MUTANTS AS IMAGING MARKER GENES
    Alexei Bogdanov; Fiscal Year: 2002
    ..The long-term goal of this research is to explore and develop novel and useful bifunctional "imaging/therapeutic marker genes" for in vivo use. ..