rhoa gtp binding protein

Summary

Summary: A RHO GTP-BINDING PROTEIN involved in regulating signal transduction pathways that control assembly of focal adhesions and actin stress fibers. This enzyme was formerly listed as EC 3.6.1.47.

Top Publications

  1. ncbi Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho-kinase)
    K Kimura
    Division of Signal Transduction, Nara Institute of Science and Technology, Ikoma 630 01, Japan
    Science 273:245-8. 1996
  2. ncbi Spatiotemporal dynamics of RhoA activity in migrating cells
    Olivier Pertz
    University of North Carolina at Chapel Hill, Department of Pharmacology and Lineberger Cancer Center, Chapel Hill, North Carolina 27599, USA
    Nature 440:1069-72. 2006
  3. ncbi Significant association of Rho/ROCK pathway with invasion and metastasis of bladder cancer
    Takao Kamai
    Department of Urology, Dokkyo University School of Medicine, Tochigi 321 0293, Japan
    Clin Cancer Res 9:2632-41. 2003
  4. ncbi Tumor necrosis factor inhibits neurite outgrowth and branching of hippocampal neurons by a rho-dependent mechanism
    Harald Neumann
    Departments of Neuroimmunology and Neurobiochemistry, Max Planck Institute of Neurobiology, 82152 Martinsried, Germany
    J Neurosci 22:854-62. 2002
  5. ncbi Neurotrophin binding to the p75 receptor modulates Rho activity and axonal outgrowth
    T Yamashita
    Department of Neurobiochemistry, Max Planck Institute of Neurobiology, Martinsried, Federal Republic of Germany
    Neuron 24:585-93. 1999
  6. pmc RhoA inactivation by p190RhoGAP regulates cell spreading and migration by promoting membrane protrusion and polarity
    W T Arthur
    Department of Cell and Developmental Biology, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA
    Mol Biol Cell 12:2711-20. 2001
  7. ncbi Regulation of TNF-alpha-induced reorganization of the actin cytoskeleton and cell-cell junctions by Rho, Rac, and Cdc42 in human endothelial cells
    B Wojciak-Stothard
    Ludwig Institute for Cancer Research, London, United Kingdom
    J Cell Physiol 176:150-65. 1998
  8. pmc Overexpression of the rhoC gene correlates with progression of ductal adenocarcinoma of the pancreas
    H Suwa
    Department of Pathology, Graduate School of Medicine, Kyoto University, Japan
    Br J Cancer 77:147-52. 1998
  9. ncbi ROCK and Dia have opposing effects on adherens junctions downstream of Rho
    Erik Sahai
    Cancer Research UK Centre for Cell and Molecular Biology, Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK
    Nat Cell Biol 4:408-15. 2002
  10. ncbi The Rho family GTPases RhoA, Rac1, and CDC42Hs regulate transcriptional activation by SRF
    C S Hill
    Transcription Laboratory, Imperial Cancer Research Fund Laboratories, London, England
    Cell 81:1159-70. 1995

Detail Information

Publications288 found, 100 shown here

  1. ncbi Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho-kinase)
    K Kimura
    Division of Signal Transduction, Nara Institute of Science and Technology, Ikoma 630 01, Japan
    Science 273:245-8. 1996
    ..Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase...
  2. ncbi Spatiotemporal dynamics of RhoA activity in migrating cells
    Olivier Pertz
    University of North Carolina at Chapel Hill, Department of Pharmacology and Lineberger Cancer Center, Chapel Hill, North Carolina 27599, USA
    Nature 440:1069-72. 2006
    ..Our data therefore show that different extracellular cues induce distinct patterns of RhoA signalling during membrane protrusion...
  3. ncbi Significant association of Rho/ROCK pathway with invasion and metastasis of bladder cancer
    Takao Kamai
    Department of Urology, Dokkyo University School of Medicine, Tochigi 321 0293, Japan
    Clin Cancer Res 9:2632-41. 2003
    ..We investigated the roles of Rho and ROCK in bladder cancer...
  4. ncbi Tumor necrosis factor inhibits neurite outgrowth and branching of hippocampal neurons by a rho-dependent mechanism
    Harald Neumann
    Departments of Neuroimmunology and Neurobiochemistry, Max Planck Institute of Neurobiology, 82152 Martinsried, Germany
    J Neurosci 22:854-62. 2002
    ..These results suggest that glia-derived TNF, as part of an injury or inflammatory process, can inhibit neurite elongation and branching during development and regeneration...
  5. ncbi Neurotrophin binding to the p75 receptor modulates Rho activity and axonal outgrowth
    T Yamashita
    Department of Neurobiochemistry, Max Planck Institute of Neurobiology, Martinsried, Federal Republic of Germany
    Neuron 24:585-93. 1999
    ..In vivo, axonal outgrowth was retarded in mice carrying a mutation in the p75NTR gene. These results indicate that p75NTR modulates in a ligand-dependent fashion the activity of intracellular proteins known to regulate actin assembly...
  6. pmc RhoA inactivation by p190RhoGAP regulates cell spreading and migration by promoting membrane protrusion and polarity
    W T Arthur
    Department of Cell and Developmental Biology, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA
    Mol Biol Cell 12:2711-20. 2001
    ..Taken together, these data demonstrate that integrin-triggered RhoA inhibition by p190RhoGAP enhances spreading and migration by regulating cell protrusion and polarity...
  7. ncbi Regulation of TNF-alpha-induced reorganization of the actin cytoskeleton and cell-cell junctions by Rho, Rac, and Cdc42 in human endothelial cells
    B Wojciak-Stothard
    Ludwig Institute for Cancer Research, London, United Kingdom
    J Cell Physiol 176:150-65. 1998
    ..Our data suggest that Cdc42, Rac, and Rho are activated in a hierarchical cascade following stimulation with TNF-alpha leading to actomyosin-mediated cell retraction and formation of intercellular gaps...
  8. pmc Overexpression of the rhoC gene correlates with progression of ductal adenocarcinoma of the pancreas
    H Suwa
    Department of Pathology, Graduate School of Medicine, Kyoto University, Japan
    Br J Cancer 77:147-52. 1998
    ..8%) cases, did not affect the expression levels in any of the rho genes. These suggest that elevated expression of the rhoC gene may be involved in the progression of pancreatic carcinoma independent of K-ras gene activation...
  9. ncbi ROCK and Dia have opposing effects on adherens junctions downstream of Rho
    Erik Sahai
    Cancer Research UK Centre for Cell and Molecular Biology, Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK
    Nat Cell Biol 4:408-15. 2002
    ..The ability of Dia1 to regulate the actin network is crucial for the localization of adherens junction components to the cell periphery...
  10. ncbi The Rho family GTPases RhoA, Rac1, and CDC42Hs regulate transcriptional activation by SRF
    C S Hill
    Transcription Laboratory, Imperial Cancer Research Fund Laboratories, London, England
    Cell 81:1159-70. 1995
    ..Functional Rho is required for regulated activity of the c-fos promoter. These results establish SRF as a nuclear target of a novel Rho-mediated signaling pathway...
  11. pmc Concentric zones of active RhoA and Cdc42 around single cell wounds
    Hélène A Benink
    Department of Zoology, University of Wisconsin Madison, Madison, WI 53706, USA
    J Cell Biol 168:429-39. 2005
    ..Each of the zones makes distinct contributions to the organization and function of the actomyosin wound array. We propose that similar rho activity zones control related processes such as cytokinesis...
  12. ncbi Anti-RhoA and anti-RhoC siRNAs inhibit the proliferation and invasiveness of MDA-MB-231 breast cancer cells in vitro and in vivo
    J Y Pillé
    Laboratoire de Recherche MERCI, EA 2122, Faculte de Medecine et Pharmacie, 76183 Rouen, France
    Mol Ther 11:267-74. 2005
    ..One may infer from these observations that specific inhibition of the Rho-signaling pathway with siRNAs represents a promising approach for the treatment of aggressive breast cancers...
  13. pmc The p160 RhoA-binding kinase ROK alpha is a member of a kinase family and is involved in the reorganization of the cytoskeleton
    T Leung
    Glaxo IMCB Group, Institute of Molecular and Cell Biology, National University of Singapore, Singapore
    Mol Cell Biol 16:5313-27. 1996
    ....
  14. ncbi A role for Rac in Tiam1-induced membrane ruffling and invasion
    F Michiels
    The Netherlands Cancer Institute, Division of Cell Biology, Amsterdam
    Nature 375:338-40. 1995
    ..Moreover, T-lymphoma cells expressing V12Rac1 become invasive, indicating that the Tiam1-Rac signalling pathway could be operating in the invasion and metastasis of tumour cells...
  15. ncbi ERK-MAPK signaling coordinately regulates activity of Rac1 and RhoA for tumor cell motility
    Emmanuel Vial
    Cancer Research UK Centre for Cell and Molecular Biology, Institute of Cancer Research, 237 Fulham Road, London, SW3 6JB, United Kingdom
    Cancer Cell 4:67-79. 2003
    ....
  16. pmc RhoA is required for monocyte tail retraction during transendothelial migration
    R A Worthylake
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    J Cell Biol 154:147-60. 2001
    ..Finally, we find that p160ROCK signaling negatively regulates integrin adhesions and that inhibition of RhoA results in an accumulation of beta2 integrin in the unretracted tails...
  17. pmc Microtubules regulate migratory polarity through Rho/ROCK signaling in T cells
    Aya Takesono
    University College London, Department of Biochemistry and Molecular Biology and Ludwig Institute for Cancer Research, London, United Kingdom
    PLoS ONE 5:e8774. 2010
    ..Microtubules (MTs) are required for persistent migration and chemotaxis, but how they affect cell polarity is not known...
  18. pmc RhoA is required for cortical retraction and rigidity during mitotic cell rounding
    Amy Shaub Maddox
    Department of Cell and Developmental Biology and Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA
    J Cell Biol 160:255-65. 2003
    ..Cumulatively, these results suggest that the mitotic increase in RhoA activity leads to rearrangements of the cortical actin cytoskeleton that promote cortical rigidity, resulting in mitotic cell rounding...
  19. ncbi Up-regulation of small GTPases, RhoA and RhoC, is associated with tumor progression in ovarian carcinoma
    Akiko Horiuchi
    Department of Obstetrics and Gynecology, Shinshu University School of Medicine, Matsumoto, Japan
    Lab Invest 83:861-70. 2003
    ..These findings suggest that up-regulation of Rho GTPases is important in the tumor progression of ovarian carcinoma and that Rho family proteins could be a molecular target in cancer therapy...
  20. pmc The fibronectin-binding integrins alpha5beta1 and alphavbeta3 differentially modulate RhoA-GTP loading, organization of cell matrix adhesions, and fibronectin fibrillogenesis
    Erik H J Danen
    Division of Cell Biology, Netherlands Cancer Institute, 1066 CX Amsterdam, Netherlands
    J Cell Biol 159:1071-86. 2002
    ..Our findings demonstrate that the pattern of fibronectin receptors expressed on a cell dictates the ability of fibronectin to stimulate RhoA-mediated organization of cell matrix adhesions...
  21. pmc The GTP binding proteins Gem and Rad are negative regulators of the Rho-Rho kinase pathway
    Yvona Ward
    Cell and Cancer Biology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    J Cell Biol 157:291-302. 2002
    ..In addition, Gem reverted the anchorage-independent growth and invasiveness of Dbl-transformed fibroblasts. These results identify physiological roles for Gem and Rad in cytoskeletal regulation mediated by ROK...
  22. pmc Reassembly of contractile actin cortex in cell blebs
    Guillaume T Charras
    Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA
    J Cell Biol 175:477-90. 2006
    ..Ezrin played no role in actin nucleation, but was essential for tethering the membrane to the cortex. The Rho pathway was important for cortex assembly in blebs...
  23. ncbi RhoA and ROCK promote migration by limiting membrane protrusions
    Rebecca A Worthylake
    Department of Cell and Developmental Biology, Lineberger Comprehensive Cancer Center and Comprehensive Center for Inflammatory Disorders, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 278:13578-84. 2003
    ..Together, our results show that RhoA/ROCK signaling promotes migration by restricting integrin activity and membrane protrusions to the leading edge...
  24. ncbi Rho and Rac but not Cdc42 regulate endothelial cell permeability
    B Wojciak-Stothard
    Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, London W1W 7BS, UK
    J Cell Sci 114:1343-55. 2001
    ..These results demonstrate that Rho and Rac act in different ways to alter endothelial barrier function, whereas Cdc42 does not affect barrier function...
  25. ncbi Signal-regulated activation of serum response factor is mediated by changes in actin dynamics
    A Sotiropoulos
    Transcription Laboratory, Imperial Cancer Research Fund Laboratories, London, United Kingdom
    Cell 98:159-69. 1999
    ..Actin treadmilling provides a convergence point for both serum- and LIM kinase-1-induced signaling to SRF...
  26. ncbi Potent activation of RhoA by Galpha q and Gq-coupled receptors
    Hiroki Chikumi
    Oral and Pharyngeal Cancer Branch, NIDCR National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892 4330, USA
    J Biol Chem 277:27130-4. 2002
    ....
  27. ncbi Sphingosine-1-phosphate, a platelet-derived lysophospholipid mediator, negatively regulates cellular Rac activity and cell migration in vascular smooth muscle cells
    Yasuji Ryu
    Department of Physiology, Kanazawa University, Graduate School of Medicine, Kanazawa, Ishikawa, Japan
    Circ Res 90:325-32. 2002
    ..These results together indicate that Edg isoform-specific, negative or positive regulation of cellular Rac activity is critically involved in S1P-mediated bimodal regulation of cell motility in SMCs and HUVECs...
  28. ncbi Palmitoylated cysteine 192 is required for RhoB tumor-suppressive and apoptotic activities
    De An Wang
    Drug Discovery Program, H Lee Moffitt Cancer Center and Research Institute, Department of Interdisciplinary Oncology, Tampa, Florida 33612, USA
    J Biol Chem 280:19243-9. 2005
    ....
  29. ncbi Mammalian diaphanous-related formin Dia1 controls the organization of E-cadherin-mediated cell-cell junctions
    Letizia Carramusa
    Department of Molecular Cell Biology, The Weizmann Institute of Science, PO Box 26, Rehovot 76100, Israel
    J Cell Sci 120:3870-82. 2007
    ..Thus, Dia1 localizes to and controls cadherin-mediated junctions in a RhoA-dependent manner...
  30. ncbi Molecular mechanism of the anti-cancer activity of cerivastatin, an inhibitor of HMG-CoA reductase, on aggressive human breast cancer cells
    Christophe Denoyelle
    Laboratoire DIFEMA, Groupe de Recherche MERCI, UFR de Medecine et de Pharmacie, 76183 Rouen, France
    Cell Signal 15:327-38. 2003
    ..Thus, the inhibition of RhoA cell signalling could be a good strategy in therapy of aggressive forms of breast cancer...
  31. ncbi Differential usage of signal transduction pathways defines two types of serum response factor target gene
    D Gineitis
    Transcription Laboratory, Imperial Cancer Research Fund Laboratories, 44 Lincoln's Inn Fields, London WC2A 3PX, United Kingdom
    J Biol Chem 276:24531-9. 2001
    ..These results show that at least two classes of SRF target genes can be distinguished on the basis of their relative sensitivity to RhoA-actin and MEK-ERK signaling pathways...
  32. pmc Ras family GTPases control growth of astrocyte processes
    D Kalman
    Department of Microbiology and Immunology, G W Hooper Foundation Laboratories, University of California at San Francisco, San Francisco, California 94143, USA
    Mol Biol Cell 10:1665-83. 1999
    ....
  33. ncbi Overexpression of RhoA is associated with poor prognosis in hepatocellular carcinoma
    X R Li
    Department of General Surgery, The Third Affiliated Hospital of Central South University, Central South University, Tongzhipo Road, Changsha, Hunan Province 410013, PR China
    Eur J Surg Oncol 32:1130-4. 2006
    ..The aim of this study was to investigate the relationship between RhoA expression and clinic pathological parameters of hepatocellular carcinoma (HCC), and to evaluate the prognostic value of RhoA for HCC patients...
  34. ncbi Oxygen activates the Rho/Rho-kinase pathway and induces RhoB and ROCK-1 expression in human and rabbit ductus arteriosus by increasing mitochondria-derived reactive oxygen species: a newly recognized mechanism for sustaining ductal constriction
    Hidemi Kajimoto
    Vascular Biology Group, Alberta Cardiovascular and Stroke Research Centre ABACUS, University of Alberta, Edmonton, Canada
    Circulation 115:1777-88. 2007
    ..Subsequent membrane depolarization and calcium influx through L-type calcium channels initiates functional closure. We hypothesize that Rho-kinase activation is an additional mechanism that sustains DA constriction...
  35. ncbi Regulation of PTEN by Rho small GTPases
    Zhong Li
    Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
    Nat Cell Biol 7:399-404. 2005
    ..Furthermore, we have identified key residues on PTEN that are required for its regulation by the small GTPase, and show that small GTPase-mediated regulation of PTEN has a significant role in the regulation of chemotaxis...
  36. ncbi Directional migration of neural crest cells in vivo is regulated by Syndecan-4/Rac1 and non-canonical Wnt signaling/RhoA
    Helen K Matthews
    Department of Anatomy and Development Biology, University College London, Gower Street, London WC1E 6BT, UK
    Development 135:1771-80. 2008
    ..In addition, we show that RhoA inhibits Rac in NC cells. We present a model in which Syn4 and PCP control directional NC migration by, at least in part, regulating membrane protrusions through the regulation of small GTPase activities...
  37. ncbi Podocalyxin activates RhoA and induces actin reorganization through NHERF1 and Ezrin in MDCK cells
    Sandra Schmieder
    Department of Cellular and Molecular Medicine, George Palade Laboratories Room 210, 9500 Gilman Drive, La Jolla, CA 92093 0651, USA
    J Am Soc Nephrol 15:2289-98. 2004
    ..PC activates RhoA through NHERF and ezrin, leading to redistribution of actin filaments. These results suggest that in podocytes, PC may also regulate foot process architecture through RhoA...
  38. pmc Tissue-specific GATA factors are transcriptional effectors of the small GTPase RhoA
    F Charron
    , , , , Canada H2W 1R7
    Genes Dev 15:2702-19. 2001
    ..The identification of transcription factor GATA-4 as a RhoA mediator in sarcomere reorganization and cardiac gene regulation provides a link between RhoA effects on transcription and cell remodeling...
  39. ncbi Phosphorylation of Neurogenin2 specifies the migration properties and the dendritic morphology of pyramidal neurons in the neocortex
    Randal Hand
    Department of Phamacology, Neuroscience Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Neuron 48:45-62. 2005
    ....
  40. ncbi Reactive oxygen species from smooth muscle mitochondria initiate cold-induced constriction of cutaneous arteries
    S R Bailey
    Davis Heart and Lung Research Institute, R 110E, The Ohio State Univ, 473 West 12th Ave, Columbus, OH 43210, USA
    Am J Physiol Heart Circ Physiol 289:H243-50. 2005
    ..Altered activity of ROS may contribute to cold-induced vasospasm occurring in Raynaud's phenomenon...
  41. pmc An SH3 domain-containing GTPase-activating protein for Rho and Cdc42 associates with focal adhesion kinase
    J D Hildebrand
    Department of Microbiology, Health Sciences Center, University of Virginia, Charlottesville 22908, USA
    Mol Cell Biol 16:3169-78. 1996
    ..We suggest that Graf may function to mediate cross talk between the tyrosine kinases such as FAK and the Rho family GTPase that control steps in integrin-initiated signaling events...
  42. ncbi PKA inhibits RhoA activation: a protection mechanism against endothelial barrier dysfunction
    Jing Qiao
    Department of Pharmacology, Rush Presbyterian St Luke s Medical Center, Chicago, Illinois 60612, USA
    Am J Physiol Lung Cell Mol Physiol 284:L972-80. 2003
    ..In conclusion, the results provide evidence that PKA inhibited RhoA activation in endothelial cells, supporting a signaling mechanism of protection against vascular endothelial barrier dysfunction...
  43. ncbi Structural insights into the interaction of ROCKI with the switch regions of RhoA
    Radovan Dvorsky
    Max Planck Institute fuer Molekulare Physiologie, Abteilung Strukturelle Biologie, Otto Hahn Strasse 11, 44227 Dortmund, Germany
    J Biol Chem 279:7098-104. 2004
    ..The identified ROCK-binding site of RhoA strikingly supports the assumption of a common consensus-binding site for effector recognition...
  44. ncbi Rho-family GTPases: it's not only Rac and Rho (and I like it)
    Krister Wennerberg
    Department of Pharmacology and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7295, USA
    J Cell Sci 117:1301-12. 2004
    ..Newer members of the family possess additional sequence elements beyond the GTPase domain, which suggests they exhibit yet other mechanisms of regulation...
  45. pmc RhoA/ROCK-mediated switching between Cdc42- and Rac1-dependent protrusion in MTLn3 carcinoma cells
    Mirvat El-Sibai
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Exp Cell Res 314:1540-52. 2008
    ..These data describe a novel role for Rho in coordinating signaling by Rac and Cdc42...
  46. pmc Rho activation patterns after spinal cord injury and the role of activated Rho in apoptosis in the central nervous system
    Catherine I Dubreuil
    Departement de pathologie et biologie cellulaire, Universite de Montreal, Montreal, QC H3T 1J4, Canada
    J Cell Biol 162:233-43. 2003
    ..Experiments with p75NTR-null mutant mice showed that immediate Rho activation after SCI is p75NTR dependent. Our results indicate that blocking overactivation of Rho after SCI protects cells from p75NTR-dependent apoptosis...
  47. ncbi Smooth muscle differentiation marker gene expression is regulated by RhoA-mediated actin polymerization
    C P Mack
    Department of Molecular Physiology and Biological Physics, University of Virginia Medical School, Charlottesville, Virginia 22908, USA
    J Biol Chem 276:341-7. 2001
    ..The results also suggest that RhoA signaling may serve as a convergence point for the multiple signaling pathways that regulate SMC differentiation...
  48. pmc Rho kinase inhibition by fasudil ameliorates diabetes-induced microvascular damage
    Ryoichi Arita
    Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan
    Diabetes 58:215-26. 2009
    ..Involvement of the Rho/Rho kinase (ROCK) pathway in diabetic microvasculopathy and therapeutic potential of fasudil, a selective ROCK inhibitor, are investigated...
  49. pmc GEF-H1 modulates localized RhoA activation during cytokinesis under the control of mitotic kinases
    Jörg Birkenfeld
    Departments of Immunology and Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Dev Cell 12:699-712. 2007
    ..Our results identify a GEF-H1-dependent mechanism to modulate localized RhoA activation during cytokinesis under the control of mitotic kinases...
  50. ncbi Activation of RhoA and inhibition of myosin phosphatase as important components in hypertension in vascular smooth muscle
    Tetsuya Seko
    First Department of Internal Medicine, Mie University School of Medicine, 2 174 Edobashi, Tsu, Mie 514 8507, Japan
    Circ Res 92:411-8. 2003
    ..These results suggest that independent of the cause of hypertension, a common point in downstream signaling and a critical component of hypertension is activation of RhoA and subsequent activation of Rho-kinase...
  51. pmc Muscle-specific signaling mechanism that links actin dynamics to serum response factor
    Koichiro Kuwahara
    Department of Molecular Biology, University of Texas Southwestern Medical Center at Dallas, 6000 Harry Hines Blvd, Dallas, TX 75390, USA
    Mol Cell Biol 25:3173-81. 2005
    ....
  52. ncbi Tumor suppressor p53 restricts Ras stimulation of RhoA and cancer cell motility
    Mingxuan Xia
    Department of Biomedical Genetics and James P Wilmot Cancer Center, University of Rochester Medical Center, Rochester, New York 14642, USA
    Nat Struct Mol Biol 14:215-23. 2007
    ..Our data suggest that p53 suppresses cancer progression to malignancy by modulating the quality of Ras signaling...
  53. ncbi PKCbeta-dependent activation of RhoA by syndecan-4 during focal adhesion formation
    Athanassios Dovas
    Division of Biomedical Sciences, Imperial College London, London, SW7 2AZ, UK
    J Cell Sci 119:2837-46. 2006
    ..All these data point to a mechanism whereby syndecan-4 signals to RhoA in a PKCalpha-dependent manner and PKCalpha directly influences RhoA activity...
  54. pmc Involvement of RhoA, ROCK I and myosin II in inverted orientation of epithelial polarity
    Wei Yu
    Department of Anatomy, Neurology, University of California, San Francisco, California 94143, USA
    EMBO Rep 9:923-9. 2008
    ..These results might be relevant to the hyperactivation of RhoA and disruption of normal polarity frequently observed in human epithelial cancers...
  55. pmc RhoA/ROCK regulation of neuritogenesis via profilin IIa-mediated control of actin stability
    Jorge Santos Da Silva
    Cavalieri Ottolenghi Scientific Institute, University of Turin, 10043 Orbassano, Torino, Italy
    J Cell Biol 162:1267-79. 2003
    ..We propose that RhoA/ROCK/PIIa-mediated regulation of actin stability, shown to be essential for neuritogenesis, may constitute a central mechanism throughout neuronal differentiation...
  56. ncbi Regulation of the polarity protein Par6 by TGFbeta receptors controls epithelial cell plasticity
    Barish Ozdamar
    Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Canada
    Science 307:1603-9. 2005
    ..These studies define how an extracellular cue signals to the polarity machinery to control epithelial cell morphology...
  57. pmc Direct activation of RhoA by reactive oxygen species requires a redox-sensitive motif
    Amir Aghajanian
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Lineberger Comprehensive Cancer Center, Chapel Hill, North Carolina, United States of America
    PLoS ONE 4:e8045. 2009
    ..We hypothesized that this redox-based mechanism occurs in cells and affects cytoskeletal dynamics, and in this report we conclude this is indeed a novel mechanism of regulating the GTPase RhoA...
  58. ncbi Genomic analysis of metastasis reveals an essential role for RhoC
    E A Clark
    Howard Hughes Medical Institute, Centre for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139, USA
    Nature 406:532-5. 2000
    ..The genomic approach allows us to identify families of genes involved in a process, not just single genes, and can indicate which molecular and cellular events might be important in complex biological processes such as metastasis...
  59. ncbi Rho GTPases are over-expressed in human tumors
    G Fritz
    Institute of Toxicology, Division of Applied Toxicology, University of Mainz, Germany
    Int J Cancer 81:682-7. 1999
    ..Overall, increase in the amount of Rho GTPases, in particular RhoA, appears to be a frequent event in different types of human tumors. This supports the view that Rho GTPases are involved in human carcinogenesis...
  60. ncbi Toxin-induced activation of Rho GTP-binding protein increases Bcl-2 expression and influences mitochondrial homeostasis
    C Fiorentini
    Department of Ultrastructures, Istituto Superiore di Sanita, Rome, Italy
    Exp Cell Res 242:341-50. 1998
    ..Altogether our results suggest a link between the Rho GTP-binding protein and the regulation of the mitochondrial homeostasis via an effect on the antiapoptotic proteins of the Bcl-2 family...
  61. ncbi Rho mediates calcium-dependent activation of p38alpha and subsequent excitotoxic cell death
    Maria M Semenova
    Department of Neurobiology, A I Virtanen Institute, University of Kuopio, Kuopio FIN 70211, Finland
    Nat Neurosci 10:436-43. 2007
    ..Therefore, Rho is an essential component of the excitotoxic cell death pathway...
  62. ncbi Rho family GTPases bind to phosphoinositide kinases
    K F Tolias
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA
    J Biol Chem 270:17656-9. 1995
    ....
  63. pmc Grit, a GTPase-activating protein for the Rho family, regulates neurite extension through association with the TrkA receptor and N-Shc and CrkL/Crk adapter molecules
    Takeshi Nakamura
    Department of Molecular Genetics, National Institute for Longevity Sciences, Program of Protecting the Brain, CREST, JST, Oobu, Aichi 474 8522, Japan
    Mol Cell Biol 22:8721-34. 2002
    ..These results suggest that Grit, a novel TrkA-interacting protein, regulates neurite outgrowth by modulating the Rho family of small GTPases...
  64. pmc The Amot/Patj/Syx signaling complex spatially controls RhoA GTPase activity in migrating endothelial cells
    Mira Ernkvist
    Department of Oncology and Pathology, Cancer Centrum Karolinska, Karolinska Institutet, Karolinska Hospital, Stockholm, Sweden
    Blood 113:244-53. 2009
    ..Taken together, our results indicate that the directional migration of capillaries in the embryo is governed by the Amot:Patj/Mupp1:Syx signaling that controls local GTPase activity...
  65. ncbi KDR stimulates endothelial cell migration through heterotrimeric G protein Gq/11-mediated activation of a small GTPase RhoA
    Huiyan Zeng
    Department of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA
    J Biol Chem 277:46791-8. 2002
    ..Taken together, our results indicate that KDR stimulates endothelial cell migration through a heterotrimeric G protein Gq/11 and Gbetagamma-mediated RhoA pathway...
  66. pmc A role for Cdc42 in macrophage chemotaxis
    W E Allen
    Muscle and Motility Research Centre, Randall Institute, King s College London WC2B 5RL, UK
    J Cell Biol 141:1147-57. 1998
    ..We conclude that Rho and Rac are required for the process of cell migration, whereas Cdc42 is required for cells to respond to a gradient of CSF-1 but is not essential for cell locomotion...
  67. ncbi Direct interaction of focal adhesion kinase with p190RhoGEF
    Jinbin Zhai
    Division of Neuropathology, University of Pennsylvania School of Medicine, Philadelphia 19104, USA
    J Biol Chem 278:24865-73. 2003
    ....
  68. ncbi Cyclic GMP-dependent protein kinase signaling pathway inhibits RhoA-induced Ca2+ sensitization of contraction in vascular smooth muscle
    V Sauzeau
    Laboratoire de Physiologie Cellulaire et Moleculaire, INSERM U 533, Faculte des Sciences, 44322 Nantes, France
    J Biol Chem 275:21722-9. 2000
    ....
  69. pmc Therapeutic potential of RhoA/Rho kinase inhibitors in pulmonary hypertension
    M Oka
    Department of Pharmacology and Center for Lung Biology, University of South Alabama, College of Medicine, Mobile, AL 36688, USA
    Br J Pharmacol 155:444-54. 2008
    ..We propose that ROCK inhibitors and other drugs that inhibit this pathway might be useful in the treatment of various forms of PH...
  70. pmc Phosphorylation states of Cdc42 and RhoA regulate their interactions with Rho GDP dissociation inhibitor and their extraction from biological membranes
    Marie Annick Forget
    Laboratoire de Medecine Moleculaire, Hôpital Sainte Justine Université du Québec à Montréal, P O Box 8888, Centre Ville Station, Montreal, Quebec, Canada H3C 3P8
    Biochem J 361:243-54. 2002
    ..We therefore suggest that phosphorylation is a mechanism of regulation of Cdc42 and RhoA activity that is independent of GDP-GTP cycling...
  71. ncbi Caspase-3-mediated cleavage of ROCK I induces MLC phosphorylation and apoptotic membrane blebbing
    M Sebbagh
    INSERM U461, Faculte de Pharmacie, 92296 Chatenay Malabry, France
    Nat Cell Biol 3:346-52. 2001
    ..We also show that expression of active truncated ROCK I induces cell blebbing. Thus, activation of ROCK I by caspase-3 seems to be responsible for bleb formation in apoptotic cells...
  72. ncbi Serine phosphorylation negatively regulates RhoA in vivo
    Shawn M Ellerbroek
    Department of Cell and Developmental Biology, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 278:19023-31. 2003
    ....
  73. pmc Vav2 activates Rac1, Cdc42, and RhoA downstream from growth factor receptors but not beta1 integrins
    B P Liu
    Department of Cell Biology and Anatomy, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7090, USA
    Mol Cell Biol 20:7160-9. 2000
    ..Expression of a carboxy-terminal fragment of Vav2 decreased the elevation of Rac1 activity induced by epidermal growth factor, consistent with Vav2 mediating activation of Rac1 downstream from growth factor receptors...
  74. pmc S100B Protein Regulates Astrocyte Shape and Migration via Interaction with Src Kinase: IMPLICATIONS FOR ASTROCYTE DEVELOPMENT, ACTIVATION, AND TUMOR GROWTH
    Flora Brozzi
    Department of Experimental Medicine and Biochemical Sciences, University of Perugia, C P 81 Succ 3, 06122 Perugia, Italy
    J Biol Chem 284:8797-811. 2009
    ....
  75. ncbi Simvastatin reduces human atrial myofibroblast proliferation independently of cholesterol lowering via inhibition of RhoA
    Karen E Porter
    Institute for Cardiovascular Research, Worsley Building, University of Leeds, Leeds LS2 9JT, UK
    Cardiovasc Res 61:745-55. 2004
    ..Although statins are known to reduce myocyte hypertrophy, their effect on cardiac fibroblast proliferation is unknown. The purpose of this study was to investigate the effects of simvastatin on human atrial myofibroblast proliferation...
  76. pmc Arp2/3 complex is important for filopodia formation, growth cone motility, and neuritogenesis in neuronal cells
    Farida Korobova
    Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA
    Mol Biol Cell 19:1561-74. 2008
    ..Consistent with this phenotype, RhoA activity was increased in Arp2/3 complex-depleted cells, indicating that besides nucleating actin filaments, Arp2/3 complex may influence cell motility by altering Rho GTPase signaling...
  77. ncbi Actin polymerization-driven molecular movement of mDia1 in living cells
    Chiharu Higashida
    Department of Pharmacology, Kyoto University Faculty of Medicine, Yoshida konoe cho, Sakyo ku, Kyoto, Japan
    Science 303:2007-10. 2004
    ..mDia1 probably moves processively along the growing end of actin filaments in cells, and Formins may be a molecular motility machinery that is independent from motor proteins...
  78. ncbi Endotoxin inhibits intestinal epithelial restitution through activation of Rho-GTPase and increased focal adhesions
    Selma Cetin
    Division of Pediatric Surgery, Department of Surgery, Children s Hospital of Pittsburgh and University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    J Biol Chem 279:24592-600. 2004
    ..We therefore conclude that endotoxin inhibits enterocyte migration through a RhoA-dependent increase in focal adhesions and enhanced cell adhesiveness, which may participate in the impaired restitution observed in experimental NEC...
  79. ncbi Structural determinants of RhoA binding and nucleotide exchange in leukemia-associated Rho guanine-nucleotide exchange factor
    Romana Kristelly
    Department of Chemistry and Biochemistry, Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, Texas 78712 0165, USA
    J Biol Chem 279:47352-62. 2004
    ..Similar changes were previously observed in structures of nucleotide-free Ras and Ef-Tu. A potential protein-docking site on the LARG PH domain is also evident and appears to be conserved throughout the Lbc subfamily of RhoGEFs...
  80. ncbi Involvement of RhoA/Rho kinase signaling in VEGF-induced endothelial cell migration and angiogenesis in vitro
    Geerten P van Nieuw Amerongen
    Laboratory for Physiology, Institute for Cardiovascular Research, VU University Medical Center, Amsterdam, The Netherlands
    Arterioscler Thromb Vasc Biol 23:211-7. 2003
    ..The small GTPase RhoA previously has been implicated in vascular endothelial growth factor (VEGF)-induced signaling pathways, but its role has not been clarified...
  81. ncbi Activation of Rho GTPases by synaptic transmission in the hippocampus
    E M O'Kane
    Division of Neuroscience and Biomedical Systems, Institute of Biomedical and Life Sciences, West Medical Building, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    J Neurochem 87:1309-12. 2003
    ..This illustrates that these GTPases are potential mediators of synaptic transmission in the hippocampus, and raises the possibility that RhoB may play a role in plasticity at hippocampal synapses during LTP...
  82. ncbi Regulation of cell polarity and protrusion formation by targeting RhoA for degradation
    Hong Rui Wang
    Program in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto M56 1x5, Canada
    Science 302:1775-9. 2003
    ..Smurf1 thus links the polarity complex to degradation of RhoA in lamellipodia and filopodia to prevent RhoA signaling during dynamic membrane movements...
  83. ncbi Regulation of Rho family GTPases by cell-cell and cell-matrix adhesion
    William T Arthur
    Department of Cell and Developmental Biology, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA
    Biol Res 35:239-46. 2002
    ....
  84. pmc SHP-2 positively regulates myogenesis by coupling to the Rho GTPase signaling pathway
    Maria I Kontaridis
    Department of Pharmacology, Yale University School of Medicine, New Haven, CT 06520, USA
    Mol Cell Biol 24:5340-52. 2004
    ..Collectively, these data provide a mechanistic basis for RhoA activation in myoblasts and demonstrate that myogenesis is critically regulated by the actions of SHP-2 on the p190-B Rho GAP/RhoA pathway...
  85. pmc p190RhoGAP is the convergence point of adhesion signals from alpha 5 beta 1 integrin and syndecan-4
    Mark D Bass
    Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, England, UK
    J Cell Biol 181:1013-26. 2008
    ..This molecular mechanism explains the cooperation between extracellular matrix receptors during cell adhesion...
  86. pmc Localized zones of Rho and Rac activities drive initiation and expansion of epithelial cell-cell adhesion
    Soichiro Yamada
    Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, USA
    J Cell Biol 178:517-27. 2007
    ..We show that zones of Rac1 and lamellipodia activity and of RhoA and actomyosin contractility are restricted to the periphery of contacting membranes and together drive initiation, expansion, and completion of cell-cell adhesion...
  87. ncbi Ca2+-dependent activation of Rho and Rho kinase in membrane depolarization-induced and receptor stimulation-induced vascular smooth muscle contraction
    Sotaro Sakurada
    Department of Physiology, Kanazawa University Graduate School of Medicine, Kanazawa, Ishikawa, Japan
    Circ Res 93:548-56. 2003
    ..Taken together, these results indicate the existence of the thus-far unrecognized Ca2+-dependent Rho stimulation mechanism in VSM. Excitatory receptor agonists are suggested to use this pathway for simulating Rho...
  88. ncbi Differential effect of Rac and Cdc42 on p38 kinase activity and cell cycle progression of nonadherent primary mouse fibroblasts
    A Philips
    Institut de Genetique Moleculaire, CNRS, UMR 5535, IFR 24, 1919 Route de Mende, 34293 Montpellier Cedex 5, France
    J Biol Chem 275:5911-7. 2000
    ..This demonstrates a novel function for Cdc42, distinct from that of Rac1, in the control of cell proliferation...
  89. ncbi The structural basis of Rho effector recognition revealed by the crystal structure of human RhoA complexed with the effector domain of PKN/PRK1
    R Maesaki
    Division of Structural Biology, Nara Institute of Science and Technology, Japan
    Mol Cell 4:793-803. 1999
    ..Sequence analysis based on the structure suggests that the ACC finger fold is widespread in Rho effector proteins...
  90. pmc Guanine nucleotide exchange factor-H1 regulates cell migration via localized activation of RhoA at the leading edge
    Perihan Nalbant
    Departments of Immunology and Microbial Science, and Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Mol Biol Cell 20:4070-82. 2009
    ..This study establishes GEF-H1 as a critical organizer of key structural and signaling components of cell migration through the localized regulation of RhoA activity at the cell leading edge...
  91. ncbi Keratinocyte differentiation is regulated by the Rho and ROCK signaling pathway
    Rachel McMullan
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom
    Curr Biol 13:2185-9. 2003
    ..Thus, these results indicate that ROCK plays a critical role in regulating the balance between proliferation and differentiation in human keratinocytes...
  92. pmc The PRK2 kinase is a potential effector target of both Rho and Rac GTPases and regulates actin cytoskeletal organization
    S Vincent
    Massachusetts General Hospital Cancer Center and Harvard Medical School, Charlestown 02129, USA
    Mol Cell Biol 17:2247-56. 1997
    ..These results indicate that p140 may be a shared kinase target of both Rho and Rac GTPases that mediates their effects on rearrangements of the actin cytoskeleton...
  93. ncbi RhoA sustains integrin alpha IIbbeta 3 adhesion contacts under high shear
    Simone M Schoenwaelder
    Department of Medicine, Australian Centre for Blood Diseases, Monash University, Box Hill Hospital, Arnold St, Box Hill, Victoria 3128, Australia
    J Biol Chem 277:14738-46. 2002
    ..These studies define a critical role for RhoA in regulating the stability of integrin alpha(IIb)beta(3) adhesion contacts under conditions of high shear stress...
  94. ncbi A role for the Rho-p160 Rho coiled-coil kinase axis in the chemokine stromal cell-derived factor-1alpha-induced lymphocyte actomyosin and microtubular organization and chemotaxis
    Miguel Vicente-Manzanares
    Servicio de Inmunologia, Hospital de la Princesa, Universidad Autonoma de Madrid, C Diego de Leon, 62, E 28006 Madrid, Spain
    J Immunol 168:400-10. 2002
    ..Our results indicate that the Rho-p160ROCK axis plays a pivotal role in the control of the cell shape as a step before lymphocyte migration toward a chemotactic gradient...
  95. pmc Rho GTPases in human breast tumours: expression and mutation analyses and correlation with clinical parameters
    G Fritz
    Institute of Toxicology, Division of Applied Toxicology, University of Mainz, Obere Zahlbacher Str 67, D 55131 Mainz, Germany
    Br J Cancer 87:635-44. 2002
    ....
  96. ncbi Apocynin-induced vasodilation involves Rho kinase inhibition but not NADPH oxidase inhibition
    Torsten Schlüter
    Department of Physiology, University of Greifswald, Greifswalder Street 11C, D17495 Greifswald, Karlsburg, Germany
    Cardiovasc Res 80:271-9. 2008
    ..Further experiments were performed in normotensive rats and in NADPH oxidase subunit knock-out mice to test if apocynin-induced vasodilation depends on NADPH oxidase inhibition at all...
  97. ncbi Rho protein inactivation induced apoptosis of cultured human endothelial cells
    Stefan Hippenstiel
    Charite, Department of Internal Medicine, Humboldt University, 13353 Berlin, Germany
    Am J Physiol Lung Cell Mol Physiol 283:L830-8. 2002
    ..Caspase-3 activity and apoptosis induced by TcdB-10463 were abolished by cAMP elevation. In summary, inhibition of Rho in endothelial cells activates caspase-9- and -3-dependent apoptosis, which can be antagonized by cAMP elevation...
  98. ncbi RhoA and RhoC proteins promote both cell proliferation and cell invasion of human oesophageal squamous cell carcinoma cell lines in vitro and in vivo
    A Faried
    Department of General Surgical Science Surgery I, Graduate School of Medicine, Gunma University, 3 39 22, Showa machi, Maebashi, Gunma, 371 8511, Japan
    Eur J Cancer 42:1455-65. 2006
    ..The results from this study suggest that active Rho proteins may induce a transforming effect that leads to a malignant phenotype...
  99. ncbi Spatial and temporal activation of the small GTPases RhoA and Rac1 by the netrin-1 receptor UNC5a during neurite outgrowth
    Mariêve Picard
    Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada H3A 2B2
    Cell Signal 21:1961-73. 2009
    ..Together, these findings demonstrate that the netrin-1 receptor UNC5a is able to induce neurite outgrowth and to differentially activate RhoA and Rac1 during neurite extension in a spatial and temporal manner...
  100. ncbi The Yersinia pseudotuberculosis cytotoxic necrotizing factor (CNFY) selectively activates RhoA
    Claudia Hoffmann
    Institut für Experimentelle und Klinische Pharmakologie und Toxikologie der Albert Ludwigs Universität Freiburg, D 79104 Freiburg, Germany
    J Biol Chem 279:16026-32. 2004
    ..Altogether, our data suggest that CNFY from Y. pseudotuberculosis is a strong, selective activator of RhoA, which can be used as a powerful tool for constitutive RhoA activation without concomitant activation of Rac1 or Cdc42...
  101. ncbi LINGO-1 is a component of the Nogo-66 receptor/p75 signaling complex
    Sha Mi
    Department of Discovery Biology, Biogen Idec, Inc, 14 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nat Neurosci 7:221-8. 2004
    ..This effect on neurons was mimicked using an exogenously added human LINGO-1-Fc fusion protein. Together these observations suggest that LINGO-1 has an important role in CNS biology...

Research Grants57

  1. Regulation of Rho Signaling by S-Nitrosothiols
    A Richard Whorton; Fiscal Year: 2012
    ..abstract_text> ..
  2. Modulation of RhoA Signaling by the mRNA Binding Protein hnRNPQ1
    KATHRYN RENAE WILLIAMS; Fiscal Year: 2013
    ..abstract_text> ..
  3. Significance and Function of HGAL in Lymphoma
    Izidore S Lossos; Fiscal Year: 2013
    ..Furthermore, these studies will reveal potentially new mechanisms regulating dissemination and progression of lymphoid tumors. These mechanistic studies may provide novel approaches for new therapeutic. ..
  4. Adrenergic activation of NHE requires PLD in CCL39 cells
    JOSEPH PROVOST; Fiscal Year: 2006
    ..All of the preliminary data was collected by undergraduates and the funding of this project would involve 15 to 20 undergraduates in meaningful research over the three-year term of the grant. [unreadable] [unreadable]..
  5. Olympus FV1000 Laser Scanning Confocal Microscope
    William Bement; Fiscal Year: 2007
    ..This information, in turn, will help guide future investigations of a number of normal and pathological human processes such as wound repair, nerve regeneration, cancer, and birth defects. [unreadable] [unreadable] [unreadable]..
  6. Effect of HIV Tat on endothelial cell function
    Lance Terada; Fiscal Year: 2007
    ..The broad objectives of this grant are to further define the nature and significance of Tat dependent oxidant signaling through Hic-5 and EG-1 in endothelial cells. ..
  7. Phagocytosis of apoptotic cells: Signaling via GULP
    Kodi Ravichandran; Fiscal Year: 2007
    ..In these mouse studies, we will determine the effect on engulfment due to the absence of GULP, or disrupted GULP function, and any potential effects on autoimmunity due to defective engulfment. ..
  8. Flow Regulation of SREBP in Atherosclerosis
    John Shyy; Fiscal Year: 2007
    ..By combining bioengineering, vascular biology, and transgenic approaches, this program will generate new insights into the molecular and mechanical bases of local flow patterns in atherogenesis. ..
  9. Posttranslational Regulation of eNOS by Estrogen in the Vagina and Clitoris
    Biljana Musicki; Fiscal Year: 2007
    ..At the same time it may advance treatments for multiple disease states associated with endothelial dysfunction and alterations in hormonal milieu. [unreadable] [unreadable] [unreadable]..
  10. Erectile Dysfunction in Type II Diabetes: Role of Adiponectin
    Kanchan Chitaley; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  11. Biology of HHV-8 interactions with host cells
    Bala Chandran; Fiscal Year: 2007
    ..Further understanding of HHV-8-cell signaling will broaden our knowledge of HHV-8 related diseases and eventually lead to new anti-HHV-8 agents, and therapeutic agents against KS. ..
  12. The Role of Annexin 2 in Mucosal Wound Healing
    Brian Babbin; Fiscal Year: 2007
    ..The candidates long term goal is to develop an academic career as a physician/scientist. [unreadable] [unreadable]..
  13. Zeiss Dynamic Laser TIRF
    Richard Minshall; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  14. The Role of Rho GTPases in Ethanol-Induced Liver Injury
    Courtney Schaffert; Fiscal Year: 2007
    ..Our studies have begun to characterize how alcohol impairs these functions. Thus, results from the proposed research could lead to effective treatment strategies in alcoholic liver disease. [unreadable] [unreadable] [unreadable]..
  15. eNOS-Protein Interaction in the Penis
    Biljana Musicki; Fiscal Year: 2007
    ..At the same time, it may suggest a specific eNOS regulatory pathway as a possible new molecular target for treating ED. [unreadable] [unreadable] [unreadable]..
  16. Caveolin-1 and NO Regulate PMN-mediated Increases in Vascular Permeability
    Richard D Minshall; Fiscal Year: 2011
    ....
  17. Versatile scaffolds to visualize endogenous protein activation in living cells
    KLAUS MICHAEL HAHN; Fiscal Year: 2010
    ..PAK, JNK, and ERK2 are each on a different, parallel pathway downstream of Src. The spatio-temporal regulation of this signaling network to produce different cellular responses will be examined. ..
  18. Molecular Mechanism of HSV Entry and Spread
    Deepak Shukla; Fiscal Year: 2009
    ..The reagents generated would also be useful for evaluating other biological functions of 3-OS HS as well. ..
  19. CD44/VARIANT CYTOSKELETON IN BREAST CANCER PROGRESSION
    LILLY YW BOURGUIGNON; Fiscal Year: 2010
    ....
  20. HSP27 Association with Thin Filaments in Colonic Muscle
    KHALIL BITAR; Fiscal Year: 2009
    ..abstract_text> ..
  21. Pregnane X Receptor as a Regulator of Multi-Drug Resistance in Prostate Cancer
    Daotai Nie; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  22. Cytoskeletal Regulation of Outer Hair Cell Motility
    Federico Kalinec; Fiscal Year: 2007
    ..abstract_text> ..
  23. Role of Acetaldehyde in Alcoholic Cardiomyopathy
    Jun Ren; Fiscal Year: 2008
    ..Our long-term goal is to establish the toxic mechanism of acetaldehyde in the development of alcoholic cardiomyopathy so that prevention and treatment can be optimized [unreadable] [unreadable]..
  24. Apoptotic Cell Recognition & Clearance 2007 Gordon Research Conference
    Kodi Ravichandran; Fiscal Year: 2007
    ..The meeting is purposely held in alternative years with[unreadable] the Apoptosis meeting to encourage participation by all of those with an interest in cell death. ..
  25. Inhibitory Signaling via FcgRIIB Receptor on B Cells
    Kodi Ravichandran; Fiscal Year: 2008
    ....
  26. NEUROTROPHINS AND CARDIOGENESIS
    Barbara Hempstead; Fiscal Year: 2002
    ....
  27. Protein Tyrosine Phosphatase and Macrophage Function
    Fiona Pixley; Fiscal Year: 2005
    ..2. To elucidate the role of PTP? in the regulation of paxillin tyrosine phosphorylation. 3. To determine the role of PTP? in mouse development and macrophage function. ..
  28. Dynamics of MAPK Phosphorylation in Living Cells
    Klaus Hahn; Fiscal Year: 2004
    ..The fundamental processes controlled by Erk, including motility, apoptosis, and proliferation, are critical to normal homeostasis and relevant to many diseases and immune function. ..
  29. Cytoskeletal Regulation of Outer Hair Cell Motility
    Federico Kalinec; Fiscal Year: 2004
    ..abstract_text> ..
  30. Microglial interactions with amyloid beta peptide
    Douglas Walker; Fiscal Year: 2003
    ..Overall, the findings from the proposed research could extend our knowledge of the inflammatory events occurring in the AD brain. ..
  31. CGMP DEPENDENT PROTEIN KINASE IN VASCULAR BIOLOGY
    HOWARD SURKS; Fiscal Year: 2003
    ..abstract_text> ..
  32. Role of 3-0-sulfotransferases in HSV entry
    Deepak Shukla; Fiscal Year: 2003
    ..abstract not supplied ..
  33. ENYZMES AFFECTING THE ACCUMULATION OF ALTERED PROTEINS
    Steven Clarke; Fiscal Year: 2003
    ..These studies will hopefully not only provide a new window to view protein life but may also suggest that the biological aging process may be closely linked to how well cells can keep polypeptides free of spontaneous covalent damage. ..
  34. SHEAR STRESS ENHANCEMENT OF SMOOTH MUSCLE CELL MIGRATION
    John Shyy; Fiscal Year: 2003
    ....
  35. STRAIN INDUCTION OF VSMC PROLIFERATION AND APOPTOSIS
    John Shyy; Fiscal Year: 2002
    ....
  36. Gordon Conference on Neurotrophic Factors (2003,2005)
    Barbara Hempstead; Fiscal Year: 2005
    ..Hempstead and Y.-A. Barde) to profile new and young investigators as speakers in this Conference and for them to have a leadership role at this meeting. ..
  37. Atypical regulation of vascular tone by protein kinase C
    Thomas Robertson; Fiscal Year: 2005
    ..As such, these studies are likely to provide insights into the functional heterogeneity of the vasculature and may identify aPKC as a candidate mediator of vascular heterogeneity. ..
  38. Cell type gene delivery and alcoholic liver disease
    Michael Wheeler; Fiscal Year: 2006
    ..In addition, through didactic training, and interactions with his mentor and key faculty, the applicant will acquire new skills that will allow him to become a successful member of the alcohol research community. ..
  39. Functional hyperemia during metabolic syndrome
    Jay Naik; Fiscal Year: 2005
    ....
  40. Intestinal Mucosal Wound Resealing by Restitution
    Brian Babbin; Fiscal Year: 2005
    ..Such studies will provide important insights into the molecular mechanisms of restitution which will provide a basis for therapeutics aimed at promoting wound closure. ..
  41. Fibronectin mediated cell migration: signal integration
    Xiang Dong Ren; Fiscal Year: 2005
    ....
  42. TGFBeta Signaling in the Bladder Stroma
    Neil Bhowmick; Fiscal Year: 2005
    ..Results from these studies are directly relevant to potential non-invasive strategies in treating one of the most common pathologies of the bladder. ..
  43. CD44-P185HER2 INTERACTION IN OVARIAN CANCER PROGRESSION
    LILLY BOURGUIGNON; Fiscal Year: 2007
    ..abstract_text> ..
  44. Ceruloplasmin Mediated Penile Endothelial Dysfunction in Type 1 Diabetes
    Kanchan Chitaley; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  45. IGF-1, Oxidative Stress and Cardiovascular Aging
    Jun Ren; Fiscal Year: 2002
    ..Our long-term goal is to establish the causal link among IGF-1 deficiency, enhanced oxidative damage and ventricular dysfunction in the progression of cardiovascular aging so that hormonal or antioxidant therapy can be optimized...
  46. Nitric Oxide-Mediated Pulmonary Vasodilation After Chronic Hypoxia
    Thomas Resta; Fiscal Year: 2007
    ..Findings from studies are expected to fundamentally advance our understanding of signal transduction mechanisms by which PKG regulates pulmonary vascular tone, as well as adaptive responses of the pulmonary circulation to CH. ..
  47. Regulation of Vascular Calcification in Diabetes
    XUENING CHEN; Fiscal Year: 2007
    ....
  48. ENVELOPE GLYCOPROTEINS OF HHV8
    Bala Chandran; Fiscal Year: 2002
    ..1(A and B) and gB will lead into an important in sight into the development of future control measures. ..
  49. Entry of HHV-8 Into the Target Cells
    Bala Chandran; Fiscal Year: 2009
    ..Further understanding of HHV-8-entry, integrin-dependent cell signaling pathways, and their role in infection would eventually lead to the development of new anti-HHV-8 agents. ..
  50. Carbon Monoxide to Prevent Circulatory Collapse
    Leo Otterbein; Fiscal Year: 2007
    ..Specific Aim #2: To test whether the protective effects of CO on shock and tissue hypoxia involve maintenance and/or regulation of the endothelium and microcirculation. ..
  51. SHORT TERM TRAINING FOR MINORITY STUDENTS
    Sharon Rounds; Fiscal Year: 2006
    ..We present results of our previous experience showing that of students trained thus far, 45 percent have gone on to MD, PhD, or MD/PhD training programs. ..
  52. Small GTPases and Lung Endothelial Apoptosis
    Sharon Rounds; Fiscal Year: 2009
    ..In addition, understanding of apoptosis may provide clues to treatment of lung diseases dependent upon abnormal endothelial proliferation, such as Pulmonary Arterial Hypertension and cancer. ..
  53. Role of DEG/ENaC in the renal myogenic response
    NIKKI JERNIGAN; Fiscal Year: 2005
    ..abstract_text> ..
  54. Stretch-Induced Changes in Endothelial Contractility
    Jeffrey Jacobson; Fiscal Year: 2003
    ..These studies, which define EC biochemical and genomic response to cyclic stretch, may result in new therapeutic strategies that target VILI. ..
  55. Anti-Inflammatory Effects of Carbon Monoxide in the Lung
    Leo Otterbein; Fiscal Year: 2006
    ..3) Evaluate the mechanism by which CO-induced activation of p38 provides cytoprotection in endotoxic shock. 4) Evaluate the rote of CO-induced iNOS in the cytoprotection and induction of the anti-inflammatory phenotype. ..