rhoa gtp binding protein

Summary

Summary: A RHO GTP-BINDING PROTEIN involved in regulating signal transduction pathways that control assembly of focal adhesions and actin stress fibers. This enzyme was formerly listed as EC 3.6.1.47.

Top Publications

  1. ncbi Regulation of TNF-alpha-induced reorganization of the actin cytoskeleton and cell-cell junctions by Rho, Rac, and Cdc42 in human endothelial cells
    B Wojciak-Stothard
    Ludwig Institute for Cancer Research, London, United Kingdom
    J Cell Physiol 176:150-65. 1998
  2. ncbi Tumor necrosis factor inhibits neurite outgrowth and branching of hippocampal neurons by a rho-dependent mechanism
    Harald Neumann
    Departments of Neuroimmunology and Neurobiochemistry, Max Planck Institute of Neurobiology, 82152 Martinsried, Germany
    J Neurosci 22:854-62. 2002
  3. pmc RhoA inactivation by p190RhoGAP regulates cell spreading and migration by promoting membrane protrusion and polarity
    W T Arthur
    Department of Cell and Developmental Biology, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA
    Mol Biol Cell 12:2711-20. 2001
  4. pmc Microtubules regulate migratory polarity through Rho/ROCK signaling in T cells
    Aya Takesono
    University College London, Department of Biochemistry and Molecular Biology and Ludwig Institute for Cancer Research, London, United Kingdom
    PLoS ONE 5:e8774. 2010
  5. ncbi A role for Rac in Tiam1-induced membrane ruffling and invasion
    F Michiels
    The Netherlands Cancer Institute, Division of Cell Biology, Amsterdam
    Nature 375:338-40. 1995
  6. ncbi Up-regulation of small GTPases, RhoA and RhoC, is associated with tumor progression in ovarian carcinoma
    Akiko Horiuchi
    Department of Obstetrics and Gynecology, Shinshu University School of Medicine, Matsumoto, Japan
    Lab Invest 83:861-70. 2003
  7. ncbi Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho-kinase)
    K Kimura
    Division of Signal Transduction, Nara Institute of Science and Technology, Ikoma 630 01, Japan
    Science 273:245-8. 1996
  8. ncbi Signal-regulated activation of serum response factor is mediated by changes in actin dynamics
    A Sotiropoulos
    Transcription Laboratory, Imperial Cancer Research Fund Laboratories, London, United Kingdom
    Cell 98:159-69. 1999
  9. ncbi Podocalyxin activates RhoA and induces actin reorganization through NHERF1 and Ezrin in MDCK cells
    Sandra Schmieder
    Department of Cellular and Molecular Medicine, George Palade Laboratories Room 210, 9500 Gilman Drive, La Jolla, CA 92093 0651, USA
    J Am Soc Nephrol 15:2289-98. 2004
  10. pmc The GTP binding proteins Gem and Rad are negative regulators of the Rho-Rho kinase pathway
    Yvona Ward
    Cell and Cancer Biology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    J Cell Biol 157:291-302. 2002

Research Grants

  1. Adrenergic activation of NHE requires PLD in CCL39 cells
    JOSEPH PROVOST; Fiscal Year: 2006
  2. Adrenergic activation of NHE requires PLD in CCL39 cells
    JOSEPH PROVOST; Fiscal Year: 2004
  3. Regulation of Rho Signaling by S-Nitrosothiols
    A Richard Whorton; Fiscal Year: 2010
  4. Regulation of Rho Signaling by S-Nitrosothiols
    A Whorton; Fiscal Year: 2009
  5. Significance and Function of HGAL in Lymphoma
    Izidore Lossos; Fiscal Year: 2009
  6. Significance and Function of HGAL in Lymphoma
    Izidore S Lossos; Fiscal Year: 2010
  7. Significance and Function of HGAL in Lymphoma
    Izidore S Lossos; Fiscal Year: 2010
  8. Endothelial Barrier Function Modulation by PKCdelta
    Elizabeth Harrington; Fiscal Year: 2004
  9. The Role of RhoA/Rho-kinase in Erectile Dysfunction in Type II Diabetes
    Liming Jin; Fiscal Year: 2007
  10. Control of Actomyosin by Microtubules
    William Bement; Fiscal Year: 2004

Detail Information

Publications257 found, 100 shown here

  1. ncbi Regulation of TNF-alpha-induced reorganization of the actin cytoskeleton and cell-cell junctions by Rho, Rac, and Cdc42 in human endothelial cells
    B Wojciak-Stothard
    Ludwig Institute for Cancer Research, London, United Kingdom
    J Cell Physiol 176:150-65. 1998
    ..Our data suggest that Cdc42, Rac, and Rho are activated in a hierarchical cascade following stimulation with TNF-alpha leading to actomyosin-mediated cell retraction and formation of intercellular gaps...
  2. ncbi Tumor necrosis factor inhibits neurite outgrowth and branching of hippocampal neurons by a rho-dependent mechanism
    Harald Neumann
    Departments of Neuroimmunology and Neurobiochemistry, Max Planck Institute of Neurobiology, 82152 Martinsried, Germany
    J Neurosci 22:854-62. 2002
    ..These results suggest that glia-derived TNF, as part of an injury or inflammatory process, can inhibit neurite elongation and branching during development and regeneration...
  3. pmc RhoA inactivation by p190RhoGAP regulates cell spreading and migration by promoting membrane protrusion and polarity
    W T Arthur
    Department of Cell and Developmental Biology, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA
    Mol Biol Cell 12:2711-20. 2001
    ..Taken together, these data demonstrate that integrin-triggered RhoA inhibition by p190RhoGAP enhances spreading and migration by regulating cell protrusion and polarity...
  4. pmc Microtubules regulate migratory polarity through Rho/ROCK signaling in T cells
    Aya Takesono
    University College London, Department of Biochemistry and Molecular Biology and Ludwig Institute for Cancer Research, London, United Kingdom
    PLoS ONE 5:e8774. 2010
    ..Microtubules (MTs) are required for persistent migration and chemotaxis, but how they affect cell polarity is not known...
  5. ncbi A role for Rac in Tiam1-induced membrane ruffling and invasion
    F Michiels
    The Netherlands Cancer Institute, Division of Cell Biology, Amsterdam
    Nature 375:338-40. 1995
    ..Moreover, T-lymphoma cells expressing V12Rac1 become invasive, indicating that the Tiam1-Rac signalling pathway could be operating in the invasion and metastasis of tumour cells...
  6. ncbi Up-regulation of small GTPases, RhoA and RhoC, is associated with tumor progression in ovarian carcinoma
    Akiko Horiuchi
    Department of Obstetrics and Gynecology, Shinshu University School of Medicine, Matsumoto, Japan
    Lab Invest 83:861-70. 2003
    ..These findings suggest that up-regulation of Rho GTPases is important in the tumor progression of ovarian carcinoma and that Rho family proteins could be a molecular target in cancer therapy...
  7. ncbi Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho-kinase)
    K Kimura
    Division of Signal Transduction, Nara Institute of Science and Technology, Ikoma 630 01, Japan
    Science 273:245-8. 1996
    ..Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase...
  8. ncbi Signal-regulated activation of serum response factor is mediated by changes in actin dynamics
    A Sotiropoulos
    Transcription Laboratory, Imperial Cancer Research Fund Laboratories, London, United Kingdom
    Cell 98:159-69. 1999
    ..Actin treadmilling provides a convergence point for both serum- and LIM kinase-1-induced signaling to SRF...
  9. ncbi Podocalyxin activates RhoA and induces actin reorganization through NHERF1 and Ezrin in MDCK cells
    Sandra Schmieder
    Department of Cellular and Molecular Medicine, George Palade Laboratories Room 210, 9500 Gilman Drive, La Jolla, CA 92093 0651, USA
    J Am Soc Nephrol 15:2289-98. 2004
    ..PC activates RhoA through NHERF and ezrin, leading to redistribution of actin filaments. These results suggest that in podocytes, PC may also regulate foot process architecture through RhoA...
  10. pmc The GTP binding proteins Gem and Rad are negative regulators of the Rho-Rho kinase pathway
    Yvona Ward
    Cell and Cancer Biology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
    J Cell Biol 157:291-302. 2002
    ..In addition, Gem reverted the anchorage-independent growth and invasiveness of Dbl-transformed fibroblasts. These results identify physiological roles for Gem and Rad in cytoskeletal regulation mediated by ROK...
  11. ncbi Regulation of the polarity protein Par6 by TGFbeta receptors controls epithelial cell plasticity
    Barish Ozdamar
    Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Canada
    Science 307:1603-9. 2005
    ..These studies define how an extracellular cue signals to the polarity machinery to control epithelial cell morphology...
  12. ncbi Directional migration of neural crest cells in vivo is regulated by Syndecan-4/Rac1 and non-canonical Wnt signaling/RhoA
    Helen K Matthews
    Department of Anatomy and Development Biology, University College London, Gower Street, London WC1E 6BT, UK
    Development 135:1771-80. 2008
    ..In addition, we show that RhoA inhibits Rac in NC cells. We present a model in which Syn4 and PCP control directional NC migration by, at least in part, regulating membrane protrusions through the regulation of small GTPase activities...
  13. ncbi Regulation of PTEN by Rho small GTPases
    Zhong Li
    Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
    Nat Cell Biol 7:399-404. 2005
    ..Furthermore, we have identified key residues on PTEN that are required for its regulation by the small GTPase, and show that small GTPase-mediated regulation of PTEN has a significant role in the regulation of chemotaxis...
  14. pmc Tissue-specific GATA factors are transcriptional effectors of the small GTPase RhoA
    F Charron
    , , , , Canada H2W 1R7
    Genes Dev 15:2702-19. 2001
    ..The identification of transcription factor GATA-4 as a RhoA mediator in sarcomere reorganization and cardiac gene regulation provides a link between RhoA effects on transcription and cell remodeling...
  15. ncbi Phosphorylation of Neurogenin2 specifies the migration properties and the dendritic morphology of pyramidal neurons in the neocortex
    Randal Hand
    Department of Phamacology, Neuroscience Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Neuron 48:45-62. 2005
    ....
  16. ncbi Endotoxin inhibits intestinal epithelial restitution through activation of Rho-GTPase and increased focal adhesions
    Selma Cetin
    Division of Pediatric Surgery, Department of Surgery, Children s Hospital of Pittsburgh and University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    J Biol Chem 279:24592-600. 2004
    ..We therefore conclude that endotoxin inhibits enterocyte migration through a RhoA-dependent increase in focal adhesions and enhanced cell adhesiveness, which may participate in the impaired restitution observed in experimental NEC...
  17. pmc Muscle-specific signaling mechanism that links actin dynamics to serum response factor
    Koichiro Kuwahara
    Department of Molecular Biology, University of Texas Southwestern Medical Center at Dallas, 6000 Harry Hines Blvd, Dallas, TX 75390, USA
    Mol Cell Biol 25:3173-81. 2005
    ....
  18. ncbi Tumor suppressor p53 restricts Ras stimulation of RhoA and cancer cell motility
    Mingxuan Xia
    Department of Biomedical Genetics and James P Wilmot Cancer Center, University of Rochester Medical Center, Rochester, New York 14642, USA
    Nat Struct Mol Biol 14:215-23. 2007
    ..Our data suggest that p53 suppresses cancer progression to malignancy by modulating the quality of Ras signaling...
  19. pmc The fibronectin-binding integrins alpha5beta1 and alphavbeta3 differentially modulate RhoA-GTP loading, organization of cell matrix adhesions, and fibronectin fibrillogenesis
    Erik H J Danen
    Division of Cell Biology, Netherlands Cancer Institute, 1066 CX Amsterdam, Netherlands
    J Cell Biol 159:1071-86. 2002
    ..Our findings demonstrate that the pattern of fibronectin receptors expressed on a cell dictates the ability of fibronectin to stimulate RhoA-mediated organization of cell matrix adhesions...
  20. ncbi Simvastatin reduces human atrial myofibroblast proliferation independently of cholesterol lowering via inhibition of RhoA
    Karen E Porter
    Institute for Cardiovascular Research, Worsley Building, University of Leeds, Leeds LS2 9JT, UK
    Cardiovasc Res 61:745-55. 2004
    ..Although statins are known to reduce myocyte hypertrophy, their effect on cardiac fibroblast proliferation is unknown. The purpose of this study was to investigate the effects of simvastatin on human atrial myofibroblast proliferation...
  21. ncbi Rho mediates calcium-dependent activation of p38alpha and subsequent excitotoxic cell death
    Maria M Semenova
    Department of Neurobiology, A I Virtanen Institute, University of Kuopio, Kuopio FIN 70211, Finland
    Nat Neurosci 10:436-43. 2007
    ..Therefore, Rho is an essential component of the excitotoxic cell death pathway...
  22. pmc Grit, a GTPase-activating protein for the Rho family, regulates neurite extension through association with the TrkA receptor and N-Shc and CrkL/Crk adapter molecules
    Takeshi Nakamura
    Department of Molecular Genetics, National Institute for Longevity Sciences, Program of Protecting the Brain, CREST, JST, Oobu, Aichi 474 8522, Japan
    Mol Cell Biol 22:8721-34. 2002
    ..These results suggest that Grit, a novel TrkA-interacting protein, regulates neurite outgrowth by modulating the Rho family of small GTPases...
  23. pmc The Amot/Patj/Syx signaling complex spatially controls RhoA GTPase activity in migrating endothelial cells
    Mira Ernkvist
    Department of Oncology and Pathology, Cancer Centrum Karolinska, Karolinska Institutet, Karolinska Hospital, Stockholm, Sweden
    Blood 113:244-53. 2009
    ..Taken together, our results indicate that the directional migration of capillaries in the embryo is governed by the Amot:Patj/Mupp1:Syx signaling that controls local GTPase activity...
  24. ncbi KDR stimulates endothelial cell migration through heterotrimeric G protein Gq/11-mediated activation of a small GTPase RhoA
    Huiyan Zeng
    Department of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA
    J Biol Chem 277:46791-8. 2002
    ..Taken together, our results indicate that KDR stimulates endothelial cell migration through a heterotrimeric G protein Gq/11 and Gbetagamma-mediated RhoA pathway...
  25. ncbi Rho family GTPases bind to phosphoinositide kinases
    K F Tolias
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA
    J Biol Chem 270:17656-9. 1995
    ....
  26. ncbi Cyclic GMP-dependent protein kinase signaling pathway inhibits RhoA-induced Ca2+ sensitization of contraction in vascular smooth muscle
    V Sauzeau
    Laboratoire de Physiologie Cellulaire et Moleculaire, INSERM U 533, Faculte des Sciences, 44322 Nantes, France
    J Biol Chem 275:21722-9. 2000
    ....
  27. pmc A role for Cdc42 in macrophage chemotaxis
    W E Allen
    Muscle and Motility Research Centre, Randall Institute, King s College London WC2B 5RL, UK
    J Cell Biol 141:1147-57. 1998
    ..We conclude that Rho and Rac are required for the process of cell migration, whereas Cdc42 is required for cells to respond to a gradient of CSF-1 but is not essential for cell locomotion...
  28. ncbi Direct interaction of focal adhesion kinase with p190RhoGEF
    Jinbin Zhai
    Division of Neuropathology, University of Pennsylvania School of Medicine, Philadelphia 19104, USA
    J Biol Chem 278:24865-73. 2003
    ....
  29. pmc Therapeutic potential of RhoA/Rho kinase inhibitors in pulmonary hypertension
    M Oka
    Department of Pharmacology and Center for Lung Biology, University of South Alabama, College of Medicine, Mobile, AL 36688, USA
    Br J Pharmacol 155:444-54. 2008
    ..We propose that ROCK inhibitors and other drugs that inhibit this pathway might be useful in the treatment of various forms of PH...
  30. ncbi Involvement of RhoA/Rho kinase signaling in VEGF-induced endothelial cell migration and angiogenesis in vitro
    Geerten P van Nieuw Amerongen
    Laboratory for Physiology, Institute for Cardiovascular Research, VU University Medical Center, Amsterdam, The Netherlands
    Arterioscler Thromb Vasc Biol 23:211-7. 2003
    ..The small GTPase RhoA previously has been implicated in vascular endothelial growth factor (VEGF)-induced signaling pathways, but its role has not been clarified...
  31. ncbi Regulation of cell polarity and protrusion formation by targeting RhoA for degradation
    Hong Rui Wang
    Program in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto M56 1x5, Canada
    Science 302:1775-9. 2003
    ..Smurf1 thus links the polarity complex to degradation of RhoA in lamellipodia and filopodia to prevent RhoA signaling during dynamic membrane movements...
  32. ncbi Activation of Rho GTPases by synaptic transmission in the hippocampus
    E M O'Kane
    Division of Neuroscience and Biomedical Systems, Institute of Biomedical and Life Sciences, West Medical Building, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
    J Neurochem 87:1309-12. 2003
    ..This illustrates that these GTPases are potential mediators of synaptic transmission in the hippocampus, and raises the possibility that RhoB may play a role in plasticity at hippocampal synapses during LTP...
  33. ncbi Genomic analysis of metastasis reveals an essential role for RhoC
    E A Clark
    Howard Hughes Medical Institute, Centre for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139, USA
    Nature 406:532-5. 2000
    ..The genomic approach allows us to identify families of genes involved in a process, not just single genes, and can indicate which molecular and cellular events might be important in complex biological processes such as metastasis...
  34. pmc p190RhoGAP is the convergence point of adhesion signals from alpha 5 beta 1 integrin and syndecan-4
    Mark D Bass
    Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, England, UK
    J Cell Biol 181:1013-26. 2008
    ..This molecular mechanism explains the cooperation between extracellular matrix receptors during cell adhesion...
  35. ncbi Regulation of Rho family GTPases by cell-cell and cell-matrix adhesion
    William T Arthur
    Department of Cell and Developmental Biology, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA
    Biol Res 35:239-46. 2002
    ....
  36. pmc SHP-2 positively regulates myogenesis by coupling to the Rho GTPase signaling pathway
    Maria I Kontaridis
    Department of Pharmacology, Yale University School of Medicine, New Haven, CT 06520, USA
    Mol Cell Biol 24:5340-52. 2004
    ..Collectively, these data provide a mechanistic basis for RhoA activation in myoblasts and demonstrate that myogenesis is critically regulated by the actions of SHP-2 on the p190-B Rho GAP/RhoA pathway...
  37. pmc Localized zones of Rho and Rac activities drive initiation and expansion of epithelial cell-cell adhesion
    Soichiro Yamada
    Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, USA
    J Cell Biol 178:517-27. 2007
    ..We show that zones of Rac1 and lamellipodia activity and of RhoA and actomyosin contractility are restricted to the periphery of contacting membranes and together drive initiation, expansion, and completion of cell-cell adhesion...
  38. ncbi Ca2+-dependent activation of Rho and Rho kinase in membrane depolarization-induced and receptor stimulation-induced vascular smooth muscle contraction
    Sotaro Sakurada
    Department of Physiology, Kanazawa University Graduate School of Medicine, Kanazawa, Ishikawa, Japan
    Circ Res 93:548-56. 2003
    ..Taken together, these results indicate the existence of the thus-far unrecognized Ca2+-dependent Rho stimulation mechanism in VSM. Excitatory receptor agonists are suggested to use this pathway for simulating Rho...
  39. ncbi Keratinocyte differentiation is regulated by the Rho and ROCK signaling pathway
    Rachel McMullan
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom
    Curr Biol 13:2185-9. 2003
    ..Thus, these results indicate that ROCK plays a critical role in regulating the balance between proliferation and differentiation in human keratinocytes...
  40. ncbi RhoA sustains integrin alpha IIbbeta 3 adhesion contacts under high shear
    Simone M Schoenwaelder
    Department of Medicine, Australian Centre for Blood Diseases, Monash University, Box Hill Hospital, Arnold St, Box Hill, Victoria 3128, Australia
    J Biol Chem 277:14738-46. 2002
    ..These studies define a critical role for RhoA in regulating the stability of integrin alpha(IIb)beta(3) adhesion contacts under conditions of high shear stress...
  41. pmc Guanine nucleotide exchange factor-H1 regulates cell migration via localized activation of RhoA at the leading edge
    Perihan Nalbant
    Departments of Immunology and Microbial Science, and Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Mol Biol Cell 20:4070-82. 2009
    ..This study establishes GEF-H1 as a critical organizer of key structural and signaling components of cell migration through the localized regulation of RhoA activity at the cell leading edge...
  42. ncbi Differential effect of Rac and Cdc42 on p38 kinase activity and cell cycle progression of nonadherent primary mouse fibroblasts
    A Philips
    Institut de Genetique Moleculaire, CNRS, UMR 5535, IFR 24, 1919 Route de Mende, 34293 Montpellier Cedex 5, France
    J Biol Chem 275:5911-7. 2000
    ..This demonstrates a novel function for Cdc42, distinct from that of Rac1, in the control of cell proliferation...
  43. pmc Rho GTPases in human breast tumours: expression and mutation analyses and correlation with clinical parameters
    G Fritz
    Institute of Toxicology, Division of Applied Toxicology, University of Mainz, Obere Zahlbacher Str 67, D 55131 Mainz, Germany
    Br J Cancer 87:635-44. 2002
    ....
  44. ncbi A role for the Rho-p160 Rho coiled-coil kinase axis in the chemokine stromal cell-derived factor-1alpha-induced lymphocyte actomyosin and microtubular organization and chemotaxis
    Miguel Vicente-Manzanares
    Servicio de Inmunologia, Hospital de la Princesa, Universidad Autonoma de Madrid, C Diego de Leon, 62, E 28006 Madrid, Spain
    J Immunol 168:400-10. 2002
    ..Our results indicate that the Rho-p160ROCK axis plays a pivotal role in the control of the cell shape as a step before lymphocyte migration toward a chemotactic gradient...
  45. pmc The PRK2 kinase is a potential effector target of both Rho and Rac GTPases and regulates actin cytoskeletal organization
    S Vincent
    Massachusetts General Hospital Cancer Center and Harvard Medical School, Charlestown 02129, USA
    Mol Cell Biol 17:2247-56. 1997
    ..These results indicate that p140 may be a shared kinase target of both Rho and Rac GTPases that mediates their effects on rearrangements of the actin cytoskeleton...
  46. ncbi The structural basis of Rho effector recognition revealed by the crystal structure of human RhoA complexed with the effector domain of PKN/PRK1
    R Maesaki
    Division of Structural Biology, Nara Institute of Science and Technology, Japan
    Mol Cell 4:793-803. 1999
    ..Sequence analysis based on the structure suggests that the ACC finger fold is widespread in Rho effector proteins...
  47. pmc Direct activation of RhoA by reactive oxygen species requires a redox-sensitive motif
    Amir Aghajanian
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Lineberger Comprehensive Cancer Center, Chapel Hill, North Carolina, United States of America
    PLoS ONE 4:e8045. 2009
    ..We hypothesized that this redox-based mechanism occurs in cells and affects cytoskeletal dynamics, and in this report we conclude this is indeed a novel mechanism of regulating the GTPase RhoA...
  48. doi Apocynin-induced vasodilation involves Rho kinase inhibition but not NADPH oxidase inhibition
    Torsten Schlüter
    Department of Physiology, University of Greifswald, Greifswalder Street 11C, D17495 Greifswald, Karlsburg, Germany
    Cardiovasc Res 80:271-9. 2008
    ..Further experiments were performed in normotensive rats and in NADPH oxidase subunit knock-out mice to test if apocynin-induced vasodilation depends on NADPH oxidase inhibition at all...
  49. pmc Rho kinase inhibition by fasudil ameliorates diabetes-induced microvascular damage
    Ryoichi Arita
    Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan
    Diabetes 58:215-26. 2009
    ..Involvement of the Rho/Rho kinase (ROCK) pathway in diabetic microvasculopathy and therapeutic potential of fasudil, a selective ROCK inhibitor, are investigated...
  50. ncbi Anti-RhoA and anti-RhoC siRNAs inhibit the proliferation and invasiveness of MDA-MB-231 breast cancer cells in vitro and in vivo
    J Y Pillé
    Laboratoire de Recherche MERCI, EA 2122, Faculte de Medecine et Pharmacie, 76183 Rouen, France
    Mol Ther 11:267-74. 2005
    ..One may infer from these observations that specific inhibition of the Rho-signaling pathway with siRNAs represents a promising approach for the treatment of aggressive breast cancers...
  51. ncbi Rho protein inactivation induced apoptosis of cultured human endothelial cells
    Stefan Hippenstiel
    Charite, Department of Internal Medicine, Humboldt University, 13353 Berlin, Germany
    Am J Physiol Lung Cell Mol Physiol 283:L830-8. 2002
    ..Caspase-3 activity and apoptosis induced by TcdB-10463 were abolished by cAMP elevation. In summary, inhibition of Rho in endothelial cells activates caspase-9- and -3-dependent apoptosis, which can be antagonized by cAMP elevation...
  52. ncbi RhoA and RhoC proteins promote both cell proliferation and cell invasion of human oesophageal squamous cell carcinoma cell lines in vitro and in vivo
    A Faried
    Department of General Surgical Science Surgery I, Graduate School of Medicine, Gunma University, 3 39 22, Showa machi, Maebashi, Gunma, 371 8511, Japan
    Eur J Cancer 42:1455-65. 2006
    ..The results from this study suggest that active Rho proteins may induce a transforming effect that leads to a malignant phenotype...
  53. ncbi LINGO-1 is a component of the Nogo-66 receptor/p75 signaling complex
    Sha Mi
    Department of Discovery Biology, Biogen Idec, Inc, 14 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nat Neurosci 7:221-8. 2004
    ..This effect on neurons was mimicked using an exogenously added human LINGO-1-Fc fusion protein. Together these observations suggest that LINGO-1 has an important role in CNS biology...
  54. ncbi The Yersinia pseudotuberculosis cytotoxic necrotizing factor (CNFY) selectively activates RhoA
    Claudia Hoffmann
    Institut für Experimentelle und Klinische Pharmakologie und Toxikologie der Albert Ludwigs Universität Freiburg, D 79104 Freiburg, Germany
    J Biol Chem 279:16026-32. 2004
    ..Altogether, our data suggest that CNFY from Y. pseudotuberculosis is a strong, selective activator of RhoA, which can be used as a powerful tool for constitutive RhoA activation without concomitant activation of Rac1 or Cdc42...
  55. ncbi Spatial and temporal activation of the small GTPases RhoA and Rac1 by the netrin-1 receptor UNC5a during neurite outgrowth
    Mariêve Picard
    Department of Anatomy and Cell Biology, McGill University, Montreal, Quebec, Canada H3A 2B2
    Cell Signal 21:1961-73. 2009
    ..Together, these findings demonstrate that the netrin-1 receptor UNC5a is able to induce neurite outgrowth and to differentially activate RhoA and Rac1 during neurite extension in a spatial and temporal manner...
  56. ncbi ROCK controls matrix synthesis in vascular smooth muscle cells: coupling vasoconstriction to vascular remodeling
    Rene Chapados
    Children s Hospital of Philadelphia, PA, USA
    Circ Res 99:837-44. 2006
    ..Finally, we showed that inhibition of ROCK activity suppresses SMC TN-C expression and disease progression in hypertensive rat PAs. Thus, in addition to its role in regulating vasoconstriction, ROCK also controls matrix production...
  57. pmc A PDZ-binding motif as a critical determinant of Rho guanine exchange factor function and cell phenotype
    Miaoliang Liu
    Angiogenesis Research Centre and Section of Cardiology, Dartmouth Medical School, Lebanon, NH 03756, USA
    Mol Biol Cell 17:1880-7. 2006
    ..We found that the activity level of JNK, which mediates transcriptional regulation downstream of RhoA, is elevated in a Syx2-dependent manner in these cells, possibly contributing to their tumorigenicity...
  58. pmc GEF-H1 modulates localized RhoA activation during cytokinesis under the control of mitotic kinases
    Jörg Birkenfeld
    Departments of Immunology and Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Dev Cell 12:699-712. 2007
    ..Our results identify a GEF-H1-dependent mechanism to modulate localized RhoA activation during cytokinesis under the control of mitotic kinases...
  59. ncbi Why three Rho proteins? RhoA, RhoB, RhoC, and cell motility
    Ann P Wheeler
    Laboratory of Cellular Molecular Biology, Ludwig Institute for Cancer Research, London W1W 7BS, United Kingdom
    Exp Cell Res 301:43-9. 2004
    ..In cancer cells, the expression and activity of RhoA, B, and C is altered in different ways. Together, this evidence suggests that although the 3 isoforms of Rho are structurally highly homologous, they have different cellular functions...
  60. pmc The Na+-H+ exchanger-1 induces cytoskeletal changes involving reciprocal RhoA and Rac1 signaling, resulting in motility and invasion in MDA-MB-435 cells
    Angelo Paradiso
    Laboratory of Clinical and Experimental Oncology, Oncology Institute of Bari, Bari, Italy
    Breast Cancer Res 6:R616-28. 2004
    ....
  61. ncbi Rho and vascular disease
    Barbara E Rolfe
    Centre for Research in Vascular Biology, School of Biomedical Sciences, University of Queensland, QLD 4072, Australia
    Atherosclerosis 183:1-16. 2005
    ..Further identification of the mechanisms by which these proteins and their effectors act in the vasculature should lead to therapies that specifically target only the adverse effects of Rho signalling...
  62. ncbi Redox regulation of RhoA
    Jongyun Heo
    Department of Biochemistry and Biophysics, The University of North Carolina, 530 Mary Ellen Jones Building, Chapel Hill, North Carolina 27599 7260, USA
    Biochemistry 45:14481-9. 2006
    ..Thus, in addition to redox agents, platinated-chemotherapeutic agents and arsenic complexes may modulate the activity of GTPases containing the GXXXCGK(S/T)C motif (i.e., RhoA and RhoB)...
  63. pmc The p160 RhoA-binding kinase ROK alpha is a member of a kinase family and is involved in the reorganization of the cytoskeleton
    T Leung
    Glaxo IMCB Group, Institute of Molecular and Cell Biology, National University of Singapore, Singapore
    Mol Cell Biol 16:5313-27. 1996
    ....
  64. ncbi Distribution of the small molecular weight GTP-binding proteins Rac1, Cdc42, RhoA and RhoB in the developing chick retina
    Andréa Silveira Santos-Bredariol
    Department of Histology and Embryology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, SP 05508 900, Brazil
    J Neurocytol 31:149-59. 2002
    ..The distribution pattern of Rho proteins during the development of the chick retina suggests a concerted role in the differentiation of specific cell types, and probably during synaptogenesis...
  65. pmc Syx, a RhoA guanine exchange factor, is essential for angiogenesis in Vivo
    Maija K Garnaas
    Department of Pediatrics, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Circ Res 103:710-6. 2008
    ..These results identify Syx as an essential contributor to angiogenesis in vivo...
  66. ncbi RhoA is involved in LFA-1 extension triggered by CXCL12 but not in a novel outside-in LFA-1 activation facilitated by CXCL9
    Ronit Pasvolsky
    Department of Immunology, Weizmann Institute of Science, Rehovot, Israel
    J Immunol 180:2815-23. 2008
    ....
  67. ncbi ERK-MAPK signaling coordinately regulates activity of Rac1 and RhoA for tumor cell motility
    Emmanuel Vial
    Cancer Research UK Centre for Cell and Molecular Biology, Institute of Cancer Research, 237 Fulham Road, London, SW3 6JB, United Kingdom
    Cancer Cell 4:67-79. 2003
    ....
  68. ncbi Human p63RhoGEF, a novel RhoA-specific guanine nucleotide exchange factor, is localized in cardiac sarcomere
    Michel Souchet
    SmithKline Beecham Laboratoires Pharmaceutiques, Unité de Biologie Cardiovasculaire, 4 rue du Chesnay Beauregard, BP 96205, 35760 Saint Grégoire, France
    J Cell Sci 115:629-40. 2002
    ..Together, these results show that p63RhoGEF is a RhoA-specific GEF that may play a key role in actin cytoskeleton reorganization in different tissues, especially in heart cellular morphology...
  69. doi Functional interactions between phosphatase POPX2 and mDia modulate RhoA pathways
    Yi Xie
    School of Biological Sciences, Nanyang Technological University, Singapore, Republic of Singapore
    J Cell Sci 121:514-21. 2008
    ..We propose that the interaction between mDia1 and POPX2 (PPM1F) serves to regulate both the actin cytoskeleton and SRF-mediated transcription, and to link the CDC42/RAC1 pathways with those of RhoA...
  70. ncbi Leukemia-associated Rho guanine nucleotide exchange factor, a Dbl family protein found mutated in leukemia, causes transformation by activation of RhoA
    G W Reuther
    Department of Pharmacology, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
    J Biol Chem 276:27145-51. 2001
    ..These data demonstrate that LARG is the first functional Dbl protein mutated in cancer and indicate LARG-mediated activation of RhoA may play a role in the development of human leukemias...
  71. ncbi RhoA prenylation is required for promotion of cell growth and transformation and cytoskeleton organization but not for induction of serum response element transcription
    C Allal
    Oncologie Cellulaire et Moléculaire, EA 2048 Université Paul Sabatier, Centre de Lutte Contre le Cancer Claudius Regaud, 20 24 rue du Pont saint Pierre, 31052 Toulouse Cedex, France
    J Biol Chem 275:31001-8. 2000
    ..These results demonstrate that RhoA geranylgeranylation is required for its biological activity and that the nature of the lipid modification is not critical...
  72. ncbi Reactive oxygen species from smooth muscle mitochondria initiate cold-induced constriction of cutaneous arteries
    S R Bailey
    Davis Heart and Lung Research Institute, R 110E, The Ohio State Univ, 473 West 12th Ave, Columbus, OH 43210, USA
    Am J Physiol Heart Circ Physiol 289:H243-50. 2005
    ..Altered activity of ROS may contribute to cold-induced vasospasm occurring in Raynaud's phenomenon...
  73. ncbi The pivotal role of RhoA GTPase in the molecular signaling of axon growth inhibition after CNS injury and targeted therapeutic strategies
    Robert E Gross
    Department of Neurosurgery, Center for Neurodegenerative Diseases, Emory University School of Medicine, Atlanta, GA 30322, USA
    Cell Transplant 16:245-62. 2007
    ..These vectors may be useful in regenerative strategies for spinal cord injury, brain injury, and neurodegenerative diseases including Parkinson's disease, Alzheimer's disease, and Huntington's disease...
  74. pmc Roles of p-ERM and Rho-ROCK signaling in lymphocyte polarity and uropod formation
    Jong Hwan Lee
    Center for Molecular Biology and Genetics, Kyoto University, Sakyo ku, Kyoto 606 8507, Japan
    J Cell Biol 167:327-37. 2004
    ..Thus, phosphorylated ERM has a potential function in establishing plasma membrane "posteriority" in the induction of the uropod in T lymphocytes...
  75. ncbi Nogo-A and myelin-associated glycoprotein mediate neurite growth inhibition by antagonistic regulation of RhoA and Rac1
    Barbara Niederost
    Institute of Medical Chemistry and Biochemistry, Leopold Franzens University of Innsbruck, A 6020 Innsbruck, Austria
    J Neurosci 22:10368-76. 2002
    ..Furthermore, we show that the recently cloned receptor for Nogo-66 and MAG, NgR, is not necessary for either NiG- or MAG-induced RhoA activation...
  76. pmc Vertebrate development requires ARVCF and p120 catenins and their interplay with RhoA and Rac
    Xiang Fang
    Department of Biochemistry and Molecular Biology, Box 117, University of Texas M D Anderson Cancer Center, 1515 Holcombe Blvd, Houston, Texas 77030 4095, USA
    J Cell Biol 165:87-98. 2004
    ..This work indicates the required functions of both p120 and ARVCF in vertebrate embryogenesis and their shared functional interplay with RhoA, Rac, and cadherin in a developmental context...
  77. ncbi The gene for a new brain specific RhoA exchange factor maps to the highly unstable chromosomal region 1p36.2-1p36.3
    M De Toledo
    , CNRS IFR24-UPR 1086, 1919 Route de Mende, 34293 Montpellier Cedex 5, France
    Oncogene 20:7307-17. 2001
    ..GEF720 is also a new candidate gene involved in the progression of neuroblastoma and developmental abnormalities associated with rearrangements in the 1p36 chromosomal region...
  78. pmc RhoE binds to ROCK I and inhibits downstream signaling
    Kirsi Riento
    Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, London, United Kingdom
    Mol Cell Biol 23:4219-29. 2003
    ..These data suggest that RhoE induces stress fiber disassembly by directly binding ROCK I and inhibiting it from phosphorylating downstream targets...
  79. ncbi Tech: a RhoA GEF selectively expressed in hippocampal and cortical neurons
    Ruth Marx
    Department of Neuroscience and Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    J Neurochem 92:850-8. 2005
    ..As Tech protein levels increase during post-natal development and remain at peak levels into adulthood, these results indicate that Tech regulates RhoA signaling pathways in developing and mature forebrain neurons...
  80. ncbi Smooth muscle differentiation marker gene expression is regulated by RhoA-mediated actin polymerization
    C P Mack
    Department of Molecular Physiology and Biological Physics, University of Virginia Medical School, Charlottesville, Virginia 22908, USA
    J Biol Chem 276:341-7. 2001
    ..The results also suggest that RhoA signaling may serve as a convergence point for the multiple signaling pathways that regulate SMC differentiation...
  81. ncbi Rho and ROCK signaling in VEGF-induced microvascular endothelial hyperpermeability
    Hengrui Sun
    Department of Surgery, University of California at Davis School of Medicine, Sacramento, California, USA
    Microcirculation 13:237-47. 2006
    ..They also investigated myosin light chain (MLC) phosphorylation and actin polymerization, two well-characterized targets of the Rho-ROCK pathway that are implicated in the regulation of endothelial barrier function...
  82. ncbi Activation of RhoA and inhibition of myosin phosphatase as important components in hypertension in vascular smooth muscle
    Tetsuya Seko
    First Department of Internal Medicine, Mie University School of Medicine, 2 174 Edobashi, Tsu, Mie 514 8507, Japan
    Circ Res 92:411-8. 2003
    ..These results suggest that independent of the cause of hypertension, a common point in downstream signaling and a critical component of hypertension is activation of RhoA and subsequent activation of Rho-kinase...
  83. ncbi Extension, retraction and contraction in the formation of a dendritic cell dendrite: distinct roles for Rho GTPases
    Claire A Swetman
    Department of Immunology and Molecular Pathology, University College London, London, GB
    Eur J Immunol 32:2074-83. 2002
    ..DC therefore coordinate adhesion and protrusion to perform a specialized process of cellular morphogenesis, which differentiates these cells from all other cells of the immune system and may contribute to their distinctive function...
  84. pmc Reassembly of contractile actin cortex in cell blebs
    Guillaume T Charras
    Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA
    J Cell Biol 175:477-90. 2006
    ..Ezrin played no role in actin nucleation, but was essential for tethering the membrane to the cortex. The Rho pathway was important for cortex assembly in blebs...
  85. pmc Mechanistic basis of Rho GTPase-induced extracellular matrix synthesis in trabecular meshwork cells
    Padmanabhan P Pattabiraman
    Dept of Ophthalmology, AERI Bldg, Rm 4010, Duke Univ School of Medicine, Durham, NC 27710, USA
    Am J Physiol Cell Physiol 298:C749-63. 2010
    ..This interaction could be significant for the homeostasis of aqueous humor drainage through the pressure-sensitive trabecular pathway...
  86. ncbi Neurotrophin binding to the p75 receptor modulates Rho activity and axonal outgrowth
    T Yamashita
    Department of Neurobiochemistry, Max Planck Institute of Neurobiology, Martinsried, Federal Republic of Germany
    Neuron 24:585-93. 1999
    ..In vivo, axonal outgrowth was retarded in mice carrying a mutation in the p75NTR gene. These results indicate that p75NTR modulates in a ligand-dependent fashion the activity of intracellular proteins known to regulate actin assembly...
  87. pmc S100B Protein Regulates Astrocyte Shape and Migration via Interaction with Src Kinase: IMPLICATIONS FOR ASTROCYTE DEVELOPMENT, ACTIVATION, AND TUMOR GROWTH
    Flora Brozzi
    Department of Experimental Medicine and Biochemical Sciences, University of Perugia, C P 81 Succ 3, 06122 Perugia, Italy
    J Biol Chem 284:8797-811. 2009
    ....
  88. ncbi Integrin engagement differentially modulates epithelial cell motility by RhoA/ROCK and PAK1
    Hua Zhou
    Department of Cell and Tissue Biology, University of California, San Francisco, California 94143, USA
    J Biol Chem 280:10624-35. 2005
    ..The data demonstrate that specific ligand-integrin pairs regulate cell motility differentially by selectively modulating activities of Rho GTPases and their effectors...
  89. ncbi Ablation of p120-catenin enhances invasion and metastasis of human lung cancer cells
    Yang Liu
    Department of Pathology, College of Basic Medical Sciences, China Medical University, Heping District, Shenyang City, Liaoning Province, China
    Cancer Sci 100:441-8. 2009
    ....
  90. ncbi beta1 integrin and organized actin filaments facilitate cardiomyocyte-specific RhoA-dependent activation of the skeletal alpha-actin promoter
    L Wei
    Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, USA
    FASEB J 15:785-96. 2001
    ..These observations support the concept that RhoA regulates SRF-dependent cardiac gene expression through cross-talk with beta1 integrin signal pathway via an organized actin cytoskeleton...
  91. ncbi Functional analysis of the contribution of RhoA and RhoC GTPases to invasive breast carcinoma
    Kaylene J Simpson
    Division of Cancer Biology and Angiogenesis, Department of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston Massachusetts 02215, USA
    Cancer Res 64:8694-701. 2004
    ..Unexpectedly, this analysis also revealed a compensatory relationship between RhoA and RhoC at the level of both their expression and activation, and a reciprocal relationship between RhoA and Rac1 activation...
  92. ncbi NGF enhances sensory axon growth induced by laminin but not by the L1 cell adhesion molecule
    Rong Yu Liu
    Neuroscience Center, University of North Carolina School of Medicine, Chapel Hill 27599 7260, USA
    Mol Cell Neurosci 20:2-12. 2002
    ..These results demonstrate a novel substrate-dependent regulation of NGF-induced growth of embryonic sensory axons mediated by RhoA GTPase...
  93. pmc Dual stimulation of Ras/mitogen-activated protein kinase and RhoA by cell adhesion to fibronectin supports growth factor-stimulated cell cycle progression
    E H Danen
    Division of Cell Biology, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands
    J Cell Biol 151:1413-22. 2000
    ..This ability of fibronectin to stimulate both Ras/MAPK- and RhoA-dependent signaling can explain its potent cooperation with growth factors in the stimulation of cell cycle progression...
  94. ncbi T-cell integrins: more than just sticking points
    Nancy Hogg
    Leukocyte Adhesion Laboratory, Cancer Research UK London Research Institute, London WC2A 3PX, UK
    J Cell Sci 116:4695-705. 2003
    ..Another important signalling pathway acts through CasL/Crk, which might regulate the activity of the GTPases Rac and Rap1 that have important roles in T-cell migration...
  95. pmc Modulation of RhoA-Rho kinase-mediated Ca2+ sensitization of rabbit myometrium during pregnancy - role of Rnd3
    C Cario-Toumaniantz
    INSERM U 533, Faculte des Sciences, 44322 Nantes, France
    J Physiol 552:403-13. 2003
    ..The RhoA-Rho kinase signalling pathway and its regulators might thus represent potential targets for the development of new treatments for pre-term labour...
  96. ncbi Protein kinase N (PKN) and PKN-related protein rhophilin as targets of small GTPase Rho
    G Watanabe
    Department of Pharmacology, Kyoto University Faculty of Medicine, Japan
    Science 271:645-8. 1996
    ..This study indicates that a serine-threonine protein kinase is a Rho effector and presents an amino acid sequence motif for binding to GTP-Rho that may be shared by a family of Rho target proteins...
  97. ncbi Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment
    Rowena McBeath
    The Cellular and Molecular Medicine Program, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Dev Cell 6:483-95. 2004
    ..These studies demonstrate that mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate...
  98. ncbi The Ras-related GTPase Rac1 binds tubulin
    A Best
    Department of Neurochemistry, Institute of Neurology, 1, Wakefield St, London WC1N 1PJ, United Kingdom
    J Biol Chem 271:3756-62. 1996
    ..These results suggest that the Rac1-tubulin interaction may play a role in Rac1 function...
  99. ncbi Coronary smooth muscle differentiation from proepicardial cells requires rhoA-mediated actin reorganization and p160 rho-kinase activity
    J Lu
    Department of Pathology, Center for Cardiovascular Development, Graduate Program in Cardiovascular Sciences, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
    Dev Biol 240:404-18. 2001
    ....
  100. pmc The insert region of RhoA is essential for Rho kinase activation and cellular transformation
    H Zong
    Department of Biochemistry and Molecular Biology and Walther Oncology Center, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA
    Mol Cell Biol 21:5287-98. 2001
    ..In conclusion, we have demonstrated that the insert region of RhoA is required for Rho kinase activation but not for binding and that this kinase activity is required to induce morphologic transformation of NIH 3T3 cells...
  101. ncbi A novel serine/threonine kinase binding the Ras-related RhoA GTPase which translocates the kinase to peripheral membranes
    T Leung
    Glaxo IMCB Group, Institute of Molecular and Cell Biology, National University of Singapore, Kent Ridge, Singapore
    J Biol Chem 270:29051-4. 1995
    ..These results are consistent with a role for the kinase ROK alpha as an effector for RhoA...

Research Grants147 found, 100 shown here

  1. Adrenergic activation of NHE requires PLD in CCL39 cells
    JOSEPH PROVOST; Fiscal Year: 2006
    ..All of the preliminary data was collected by undergraduates and the funding of this project would involve 15 to 20 undergraduates in meaningful research over the three-year term of the grant. [unreadable] [unreadable]..
  2. Adrenergic activation of NHE requires PLD in CCL39 cells
    JOSEPH PROVOST; Fiscal Year: 2004
    ..In fact all of the preliminary data was collected by undergraduates, and the funding of this project would involve 10 to 16 undergraduate students each year over the three-year term of the proposal. ..
  3. Regulation of Rho Signaling by S-Nitrosothiols
    A Richard Whorton; Fiscal Year: 2010
    ..Results from our studies will provide insight into prevention and development of potential therapeutic strategies. ..
  4. Regulation of Rho Signaling by S-Nitrosothiols
    A Whorton; Fiscal Year: 2009
    ..Results from our studies will provide insight into prevention and development of potential therapeutic strategies. ..
  5. Significance and Function of HGAL in Lymphoma
    Izidore Lossos; Fiscal Year: 2009
    ..These findings will not only elucidate the mechanisms of tumor dissemination but also will identify a new potential molecular target for novel therapeutic intervention. ..
  6. Significance and Function of HGAL in Lymphoma
    Izidore S Lossos; Fiscal Year: 2010
    ..These findings will not only elucidate the mechanisms of tumor dissemination but also will identify a new potential molecular target for novel therapeutic intervention. ..
  7. Significance and Function of HGAL in Lymphoma
    Izidore S Lossos; Fiscal Year: 2010
    ..These findings will not only elucidate the mechanisms of tumor dissemination but also will identify a new potential molecular target for novel therapeutic intervention. ..
  8. Endothelial Barrier Function Modulation by PKCdelta
    Elizabeth Harrington; Fiscal Year: 2004
    ..The elucidation of the molecular mechanisms by which PKCDELTA decreases endothelial monolayer permeability may lead to the development of therapeutic agents which are protective against endothelial barrier dysfunction. ..
  9. The Role of RhoA/Rho-kinase in Erectile Dysfunction in Type II Diabetes
    Liming Jin; Fiscal Year: 2007
    ..Understanding the molecular mechanisms of RhoA pathway regulating erectile function will provide a new therapeutic approach for treatment of ED. [unreadable] [unreadable] [unreadable]..
  10. Control of Actomyosin by Microtubules
    William Bement; Fiscal Year: 2004
    ..Thus, the proposed work will provide important and novel insights into the means by which microtubules control actomyosin in vivo. ..
  11. Endothelial Barrier Function Modulation by PKCdelta
    Elizabeth Harrington; Fiscal Year: 2003
    ..The elucidation of the molecular mechanisms by which PKCDELTA decreases endothelial monolayer permeability may lead to the development of therapeutic agents which are protective against endothelial barrier dysfunction. ..
  12. Actin-microtubule cross-talk in transient cell arrays
    William Bement; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  13. Control of Actomyosin by Microtubules
    William Bement; Fiscal Year: 2001
    ..Thus, the proposed work will provide important and novel insights into the means by which microtubules control actomyosin in vivo. ..
  14. Endothelial Barrier Function Modulation by PKCdelta
    Elizabeth Harrington; Fiscal Year: 2009
    ..We hope that identification of proteins important in maintaining a non-leaky state will assist in discovering a treatment for patients suffering from lung edema. ..
  15. Control of Actomyosin by Microtubules
    William Bement; Fiscal Year: 2002
    ..Thus, the proposed work will provide important and novel insights into the means by which microtubules control actomyosin in vivo. ..
  16. Endothelial Barrier Function Modulation by PKCdelta
    Elizabeth Harrington; Fiscal Year: 2001
    ..The elucidation of the molecular mechanisms by which PKCDELTA decreases endothelial monolayer permeability may lead to the development of therapeutic agents which are protective against endothelial barrier dysfunction. ..
  17. Actin-microtubule cross-talk in transient cell arrays
    William Bement; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  18. Endothelial Barrier Function Modulation by PKCdelta
    Elizabeth Harrington; Fiscal Year: 2002
    ..The elucidation of the molecular mechanisms by which PKCDELTA decreases endothelial monolayer permeability may lead to the development of therapeutic agents which are protective against endothelial barrier dysfunction. ..
  19. Endothelial Barrier Function Modulation by PKCdelta
    Elizabeth Harrington; Fiscal Year: 2007
    ..We hope that identification of proteins important in maintaining a non-leaky state will assist in discovering a treatment for patients suffering from lung edema. ..
  20. Control of Actomyosin by Microtubules
    William Bement; Fiscal Year: 2003
    ..Thus, the proposed work will provide important and novel insights into the means by which microtubules control actomyosin in vivo. ..
  21. Actin-microtubule cross-talk in transient cell arrays
    William Bement; Fiscal Year: 2005
    ..The successful completion of the proposed experiments will not only answer basic questions in biology, it will also provide critical insights into processes such as wound repair, cancer, birth defects and human infertility. ..
  22. Transient cytoskeletal arrays
    William Bement; Fiscal Year: 2010
    ..This work will provide cellular and molecular insights into these processes which will permit informed design of clinical interventions for these diseases. ..
  23. Endothelial Barrier Function Modulation by PKCdelta
    Elizabeth Harrington; Fiscal Year: 2008
    ..We hope that identification of proteins important in maintaining a non-leaky state will assist in discovering a treatment for patients suffering from lung edema. ..
  24. Transient cytoskeletal arrays
    William Bement; Fiscal Year: 2010
    ..This work will provide cellular and molecular insights into these processes which will permit informed design of clinical interventions for these diseases. ..
  25. FUNCTIONAL/MOLECULAR CONTROL OF ACTOMYOSIN--MICROTUBULE
    William Bement; Fiscal Year: 1999
    ..3) Cytoskeletal reorganization during cortical flow will be monitored by immunofluorescence and confocal microscopy. 4) Finally, he will determine the effect of MAPs on cortical flow by identifying Xenopus MAP(s). ..
  26. Actin-microtubule cross-talk in transient cell arrays
    William Bement; Fiscal Year: 2006
    ..unreadable] [unreadable]..
  27. Olympus FV1000 Laser Scanning Confocal Microscope
    William Bement; Fiscal Year: 2007
    ..This information, in turn, will help guide future investigations of a number of normal and pathological human processes such as wound repair, nerve regeneration, cancer, and birth defects. [unreadable] [unreadable] [unreadable]..
  28. Endothelial Barrier Function Modulation by PKCdelta
    Elizabeth O Harrington; Fiscal Year: 2010
    ..We hope that identification of proteins important in maintaining a non-leaky state will assist in discovering a treatment for patients suffering from lung edema. ..
  29. IGF-1, Oxidative Stress and Cardiovascular Aging
    Jun Ren; Fiscal Year: 2002
    ..Our long-term goal is to establish the causal link among IGF-1 deficiency, enhanced oxidative damage and ventricular dysfunction in the progression of cardiovascular aging so that hormonal or antioxidant therapy can be optimized...
  30. Ceruloplasmin Mediated Penile Endothelial Dysfunction in Type 1 Diabetes
    Kanchan Chitaley; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  31. Dynamics of MAPK Phosphorylation in Living Cells
    Klaus Hahn; Fiscal Year: 2003
    ..The fundamental processes controlled by Erk, including motility, apoptosis, and proliferation, are critical to normal homeostasis and relevant to many diseases and immune function. ..
  32. Hsp27 Association with Contractile Proteins
    KHALIL BITAR; Fiscal Year: 2003
    ....
  33. FOCAL ADHESION COMPLEXES AND LUNG ENDOTHELIAL APOPTOSIS
    Sharon Rounds; Fiscal Year: 2001
    ..Understanding of endothelial apoptosis will allow development of means of regulating vascular injury and repair. ..
  34. HSP27 Association with Thin Filaments in Colonic Muscle
    KHALIL BITAR; Fiscal Year: 2007
    ..abstract_text> ..
  35. CD44-P185HER2 INTERACTION IN OVARIAN CANCER PROGRESSION
    LILLY BOURGUIGNON; Fiscal Year: 2007
    ..abstract_text> ..
  36. PKC/PKA Regulation in Prostate Cancer by SSeCKS/Gravin
    Irwin Gelman; Fiscal Year: 2002
    ..abstract_text> ..
  37. Role of Shc in T lymphocyte development and function
    Kodi Ravichandran; Fiscal Year: 2005
    ..Since Shc has been linked to growth factor and cytokine signaling in lymphocytes and many other cell lineages, these studies would also have a broader importance for understanding signaling pathways affecting growth and differentiation. ..
  38. Small GTPases and Lung Endothelial Apoptosis
    Sharon Rounds; Fiscal Year: 2006
    ..In addition, understanding of apoptosis may provide clues to treatment of lung diseases dependent upon abnormal endothelial proliferation, such as Pulmonary Arterial Hypertension and cancer. [unreadable] [unreadable] [unreadable]..
  39. Entry of HHV-8 Into the Target Cells
    Bala Chandran; Fiscal Year: 2006
    ..Further understanding of HHV-8-entry, integrin-dependent cell signaling pathways, and their role in infection would eventually lead to the development of new anti-HHV-8 agents. ..
  40. NO-Mediated Pulmonary Vasodilation After Chronic Hypoxia
    Thomas Resta; Fiscal Year: 2005
    ..Findings from studies are expected to fundamentally advance our understanding of signal transduction mechanisms by which PKG regulates pulmonary vascular tone, as well as adaptive responses of the pulmonary circulation to CH. ..
  41. Biology of HHV-8 interactions with host cells
    Bala Chandran; Fiscal Year: 2005
    ..Further understanding of HHV-8-cell signaling will broaden our knowledge of HHV-8 related diseases and eventually lead to new anti-HHV-8 agents, and therapeutic agents against KS. ..
  42. Acute Ethanol-Induced Innate Immune Response in Liver
    Michael Wheeler; Fiscal Year: 2003
    ..The aims below will use gene delivery techniques and knockout mouse technology to address these critical questions related to roles of LPS, PI3 kinase activation, and CD14 expression in both acute ethanol and chronic ethanol toxicity. ..
  43. Ceruloplasmin Mediated Penile Endothelial Dysfunction in Type 1 Diabetes
    Kanchan Chitaley; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  44. INHIBITORY SIGNALING VIA THE FCGRIIB RECEPTOR ON B CELLS
    Kodi Ravichandran; Fiscal Year: 2000
    ..A detailed molecular understanding of FcgammaRIIB-mediated inhibitory signaling may yield important clues to B cell activation and desensitization, and point to possible future therapeutic interventions in certain autoimmune states. ..
  45. FoxM1 regulates endothelial repair following lung vascular injury
    You Yang Zhao; Fiscal Year: 2010
    ..We will also examine following LPS-induced vascular injury the ultrastructural defects and the cellular nature of the leaks and repair failure of the pulmonary vasculature in mice with endothelial cell- restricted deficiency of FoxM1. ..
  46. Versatile scaffolds to visualize endogenous protein activation in living cells
    Klaus Hahn; Fiscal Year: 2009
    ..PAK, JNK, and ERK2 are each on a different, parallel pathway downstream of Src. The spatio-temporal regulation of this signaling network to produce different cellular responses will be examined. ..
  47. SIGNALING VIA SHC AND THE INOSITOL PHOSPHATASE, SHIP
    Kodi Ravichandran; Fiscal Year: 2000
    ....
  48. STRAIN INDUCTION OF VSMC PROLIFERATION AND APOPTOSIS
    John Shyy; Fiscal Year: 1999
    ....
  49. Role of ELMO and Dock 180 in signaling to engulfment
    Kodi Ravichandran; Fiscal Year: 2009
    ..Taken together, through these studies, we expect to gain a better molecular understanding of the fundamentally important process of clearing apoptotic cells and define the role of specific players during engulfment. ..
  50. Shear Stress, AMPK, and Endothelial Functions
    John Y J Shyy; Fiscal Year: 2010
    ..The proposal will significantly increase our understanding of mechanisms by which blood flow patterns interplay with hyperlipidemia in atherogenesis, which are likely to contribute to novel therapies for its prevention and/or treatment. ..
  51. NEUROHUMORAL CONTROL OF INTERNAL ANAL SPHINCTER
    Satish Rattan; Fiscal Year: 1990
    ..The proposed studies should provide important information on the physiology and pharmacology of the IAS which may be useful in the understanding of pathophysiology, the effects of various drugs and the treatment of anorectal disorders...
  52. Dynamics of MAPK Phosphorylation in Living Cells
    Klaus Hahn; Fiscal Year: 2001
    ..The fundamental processes controlled by Erk, including motility, apoptosis, and proliferation, are critical to normal homeostasis and relevant to many diseases and immune function. ..
  53. UW Northern Rockies Regional INBRE
    Jun Ren; Fiscal Year: 2008
    ..abstract_text> ..
  54. CTGF-interacting proteins in scleroderma lung fibrosis
    GALINA BOGATKEVICH; Fiscal Year: 2008
    ..The identified protein(s) might serve then as potential target(s) in the treatment of scleroderma pulmonary fibrosis for which no proven, effective therapy currently exists. ..
  55. CD44/VARIANT CYTOSKELETON IN BREAST CANCER PROGRESSION
    LILLY BOURGUIGNON; Fiscal Year: 2002
    ..abstract_text> ..
  56. FUNCTION OF GUT PEPTIDES IN ISOLATED GUT MUSCLE CELLS
    S KARNAM; Fiscal Year: 2008
    ..Each specific aim is supported by substantial preliminary studies. Their completion should advance our understanding of the molecular mechanisms mediating smooth muscle relaxation. ..
  57. Caveolin-1 and NO Regulate PMN-mediated Increases in Vascular Permeability
    Richard Minshall; Fiscal Year: 2008
    ....
  58. PKC/PKA Regulation in Prostate Cancer by SSeCKS/Gravin
    Irwin Gelman; Fiscal Year: 2002
    ..abstract_text> ..
  59. CORE--Imaging and Physiology Core
    Richard Minshall; Fiscal Year: 2007
    ..Core D personnel, who have extensive training and experience in the described procedures, will provide the support and resources outlined below as well as the necessary training of personnel in all Projects. ..
  60. Regulation of Endothelial ICAM-1 and PMN-Mediated Lung Injury by Actin Dynamics
    Fabeha Fazal; Fiscal Year: 2010
    ....
  61. Antigens of Kaposi's sarcoma associated herpesvirus
    Bala Chandran; Fiscal Year: 2005
    ..These studies are significant since they will provide an insight into the biology of HHV-8 and it's role in the pathogenesis of KS. ..
  62. Caveolin-1 and NO Regulate PMN-mediated Increases in Vascular Permeability
    Richard Minshall; Fiscal Year: 2009
    ..Project Description Page 6 ..
  63. NO-Mediated Pulmonary Vasodilation After Chronic Hypoxia
    Thomas Resta; Fiscal Year: 2006
    ..Findings from studies are expected to fundamentally advance our understanding of signal transduction mechanisms by which PKG regulates pulmonary vascular tone, as well as adaptive responses of the pulmonary circulation to CH. ..
  64. Antigens of Kaposi's sarcoma associated herpesvirus
    Bala Chandran; Fiscal Year: 2009
    ....
  65. Pulmonary Vasoreactivity Following Chronic Hypoxia
    Thomas Resta; Fiscal Year: 2009
    ..Such disease states cause poor oxygenation of the blood (hypoxia), and the resultant pulmonary hypertension leads to right heart failure and mortality in humans. ..
  66. Entry of HHV-8 Into the Target Cells
    Bala Chandran; Fiscal Year: 2007
    ..Further understanding of HHV-8-entry, integrin-dependent cell signaling pathways, and their role in infection would eventually lead to the development of new anti-HHV-8 agents. ..
  67. Nitric Oxide-Mediated Pulmonary Vasodilation After Chronic Hypoxia
    Thomas Resta; Fiscal Year: 2007
    ..Findings from studies are expected to fundamentally advance our understanding of signal transduction mechanisms by which PKG regulates pulmonary vascular tone, as well as adaptive responses of the pulmonary circulation to CH. ..
  68. Genetic Variation in KIBRA and its role in Human Episodic Memory
    MATTHEW HUENTELMAN; Fiscal Year: 2008
    ....
  69. Regulation of Vascular Calcification in Diabetes
    XUENING CHEN; Fiscal Year: 2007
    ....
  70. FoxM1 regulates endothelial repair following lung vascular injury
    You Yang Zhao; Fiscal Year: 2007
    ..We will also examine following LPS-induced vascular injury the ultrastructural defects and the cellular nature of the leaks and repair failure of the pulmonary vasculature in mice with endothelial cell- restricted deficiency of FoxM1. ..
  71. Antigens of Kaposi's sarcoma associated herpesvirus
    Bala Chandran; Fiscal Year: 2005
    ..These studies are significant since they will provide an insight into the biology of HHV-8 and it's role in the pathogenesis of KS. ..
  72. NEUROHUMORAL CONTROL OF INTERNAL ANAL SPHINCTER
    Satish Rattan; Fiscal Year: 2005
    ..Information produced by these studies will permit an understanding of the pathophysiology that underlies disorders of the LAS, and provide a rationale for development of therapies to treat disorders of the IAS. ..
  73. Antigens of Kaposi's sarcoma associated herpesvirus
    Bala Chandran; Fiscal Year: 2003
    ..These studies are significant since they will provide an insight into the biology of HHV-8 and it's role in the pathogenesis of KS. ..
  74. NEUROHUMORAL CONTROL OF INTERNAL ANAL SPHINCTER
    Satish Rattan; Fiscal Year: 2003
    ..Information produced by these studies will permit an understanding of the pathophysiology that underlies disorders of the LAS, and provide a rationale for development of therapies to treat disorders of the IAS. ..
  75. Biology of HHV-8 interactions with host cells
    Bala Chandran; Fiscal Year: 2004
    ..Further understanding of HHV-8-cell signaling will broaden our knowledge of HHV-8 related diseases and eventually lead to new anti-HHV-8 agents, and therapeutic agents against KS. ..
  76. ENVELOPE GLYCOPROTEINS OF HHV8
    Bala Chandran; Fiscal Year: 2000
    ..1(A and B) and gB will lead into an important in sight into the development of future control measures. ..
  77. Antigens of Kaposi's sarcoma associated herpesvirus
    Bala Chandran; Fiscal Year: 2004
    ..These studies are significant since they will provide an insight into the biology of HHV-8 and it's role in the pathogenesis of KS. ..
  78. NEUROHUMORAL CONTROL OF INTERNAL ANAL SPHINCTER
    Satish Rattan; Fiscal Year: 2001
    ..The proposed studies should provide important information leading to a better understanding of the pathophysiology and the treatment of anorectal disorders. ..
  79. Antigens of Kaposi's sarcoma associated herpesvirus
    Bala Chandran; Fiscal Year: 2002
    ..These studies are significant since they will provide an insight into the biology of HHV-8 and it's role in the pathogenesis of KS. ..
  80. ENVELOPE GLYCOPROTEINS OF HHV8
    Bala Chandran; Fiscal Year: 2002
    ..1(A and B) and gB will lead into an important in sight into the development of future control measures. ..
  81. NEUROHUMORAL CONTROL OF INTERNAL ANAL SPHINCTER
    Satish Rattan; Fiscal Year: 2000
    ..The proposed studies should provide important information leading to a better understanding of the pathophysiology and the treatment of anorectal disorders. ..
  82. ANTIGENS OF KAPOSIS SARCOMA ASSOCIATED HERPESVIRUS
    Bala Chandran; Fiscal Year: 2001
    ..These studies could be significant since if HHV-8 replication precedes the development of KS then this may have important implications in the assessment of therapeutic intervention of HHV-8 infection by anti-herpes viral drugs. ..
  83. NEUROHUMORAL CONTROL OF INTERNAL ANAL SPHINCTER
    Satish Rattan; Fiscal Year: 2009
    ..This information will have significant implications in the pathophysiology and therapeutic potentials of the IAS dysfunction. ..
  84. NEUROHUMORAL CONTROL OF INTERNAL ANAL SPHINCTER
    Satish Rattan; Fiscal Year: 2008
    ..This information will have significant implications in the pathophysiology and therapeutic potentials of the IAS dysfunction. ..
  85. NEUROHUMORAL CONTROL OF INTERNAL ANAL SPHINCTER
    Satish Rattan; Fiscal Year: 2002
    ..Information produced by these studies will permit an understanding of the pathophysiology that underlies disorders of the LAS, and provide a rationale for development of therapies to treat disorders of the IAS. ..
  86. Entry of HHV-8 Into the Target Cells
    Bala Chandran; Fiscal Year: 2009
    ..Further understanding of HHV-8-entry, integrin-dependent cell signaling pathways, and their role in infection would eventually lead to the development of new anti-HHV-8 agents. ..
  87. FoxM1 regulates endothelial repair following lung vascular injury
    You Yang Zhao; Fiscal Year: 2008
    ..We will also examine following LPS-induced vascular injury the ultrastructural defects and the cellular nature of the leaks and repair failure of the pulmonary vasculature in mice with endothelial cell- restricted deficiency of FoxM1. ..
  88. Genetic Variation in KIBRA and its role in Human Episodic Memory
    MATTHEW HUENTELMAN; Fiscal Year: 2009
    ....
  89. NEUROHUMORAL CONTROL OF INTERNAL ANAL SPINCHTER
    Satish Rattan; Fiscal Year: 1991
    ..The proposed studies should provide important information on the physiology and pharmacology of the IAS which may be useful in the understanding of pathophysiology, the effects of various drugs and the treatment of anorectal disorders...
  90. NEUROHUMORAL CONTROL OF INTERNAL ANAL SPHINCTER
    Satish Rattan; Fiscal Year: 1999
    ..The proposed studies should provide important information leading to a better understanding of the pathophysiology and the treatment of anorectal disorders. ..
  91. Entry of HHV-8 Into the Target Cells
    Bala Chandran; Fiscal Year: 2008
    ..Further understanding of HHV-8-entry, integrin-dependent cell signaling pathways, and their role in infection would eventually lead to the development of new anti-HHV-8 agents. ..
  92. Src Regulation of Lung Endothelial Barrier Function
    Richard Minshall; Fiscal Year: 2005
    ..Thus, these studies will elucidate the Src-dependent signaling mechanisms that regulate endocytosis and transport across the pulmonary vascular endothelial barrier. ..
  93. Antigens of Kaposi's sarcoma associated herpesvirus
    Bala Chandran; Fiscal Year: 2010
    ....
  94. Entry of HHV-8 Into the Target Cells
    Bala Chandran; Fiscal Year: 2005
    ..Further understanding of HHV-8-entry, integrin-dependent cell signaling pathways, and their role in infection would eventually lead to the development of new anti-HHV-8 agents. ..