rhoa gtp binding protein

Summary

Summary: A RHO GTP-BINDING PROTEIN involved in regulating signal transduction pathways that control assembly of focal adhesions and actin stress fibers. This enzyme was formerly listed as EC 3.6.1.47.

Top Publications

  1. ncbi Regulation of the polarity protein Par6 by TGFbeta receptors controls epithelial cell plasticity
    Barish Ozdamar
    Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Canada
    Science 307:1603-9. 2005
  2. ncbi Microtubules regulate migratory polarity through Rho/ROCK signaling in T cells
    Aya Takesono
    University College London, Department of Biochemistry and Molecular Biology and Ludwig Institute for Cancer Research, London, United Kingdom
    PLoS ONE 5:e8774. 2010
  3. ncbi Signal-regulated activation of serum response factor is mediated by changes in actin dynamics
    A Sotiropoulos
    Transcription Laboratory, Imperial Cancer Research Fund Laboratories, London, United Kingdom
    Cell 98:159-69. 1999
  4. ncbi Phosphorylation of Neurogenin2 specifies the migration properties and the dendritic morphology of pyramidal neurons in the neocortex
    Randal Hand
    Department of Phamacology, Neuroscience Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Neuron 48:45-62. 2005
  5. ncbi Tissue-specific GATA factors are transcriptional effectors of the small GTPase RhoA
    F Charron
    , , , , Canada H2W 1R7
    Genes Dev 15:2702-19. 2001
  6. ncbi Reassembly of contractile actin cortex in cell blebs
    Guillaume T Charras
    Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA
    J Cell Biol 175:477-90. 2006
  7. ncbi Muscle-specific signaling mechanism that links actin dynamics to serum response factor
    Koichiro Kuwahara
    Department of Molecular Biology, University of Texas Southwestern Medical Center at Dallas, 6000 Harry Hines Blvd, Dallas, TX 75390, USA
    Mol Cell Biol 25:3173-81. 2005
  8. ncbi Tumor suppressor p53 restricts Ras stimulation of RhoA and cancer cell motility
    Mingxuan Xia
    Department of Biomedical Genetics and James P Wilmot Cancer Center, University of Rochester Medical Center, Rochester, New York 14642, USA
    Nat Struct Mol Biol 14:215-23. 2007
  9. ncbi Direct interaction of focal adhesion kinase with p190RhoGEF
    Jinbin Zhai
    Division of Neuropathology, University of Pennsylvania School of Medicine, Philadelphia 19104, USA
    J Biol Chem 278:24865-73. 2003
  10. ncbi Cyclic GMP-dependent protein kinase signaling pathway inhibits RhoA-induced Ca2+ sensitization of contraction in vascular smooth muscle
    V Sauzeau
    Laboratoire de Physiologie Cellulaire et Moleculaire, INSERM U 533, Faculte des Sciences, 44322 Nantes, France
    J Biol Chem 275:21722-9. 2000

Research Grants

  1. Adrenergic activation of NHE requires PLD in CCL39 cells
    JOSEPH PROVOST; Fiscal Year: 2006
  2. Regulation of Rho Signaling by S-Nitrosothiols
    A Richard Whorton; Fiscal Year: 2010
  3. Significance and Function of HGAL in Lymphoma
    Izidore Lossos; Fiscal Year: 2009
  4. Significance and Function of HGAL in Lymphoma
    Izidore S Lossos; Fiscal Year: 2010
  5. Significance and Function of HGAL in Lymphoma
    Izidore S Lossos; Fiscal Year: 2010
  6. Endothelial Barrier Function Modulation by PKCdelta
    Elizabeth Harrington; Fiscal Year: 2004
  7. The Role of RhoA/Rho-kinase in Erectile Dysfunction in Type II Diabetes
    Liming Jin; Fiscal Year: 2007
  8. Control of Actomyosin by Microtubules
    William Bement; Fiscal Year: 2004
  9. Endothelial Barrier Function Modulation by PKCdelta
    Elizabeth Harrington; Fiscal Year: 2009
  10. Actin-microtubule cross-talk in transient cell arrays
    William Bement; Fiscal Year: 2007

Detail Information

Publications222 found, 100 shown here

  1. ncbi Regulation of the polarity protein Par6 by TGFbeta receptors controls epithelial cell plasticity
    Barish Ozdamar
    Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Canada
    Science 307:1603-9. 2005
    ..These studies define how an extracellular cue signals to the polarity machinery to control epithelial cell morphology...
  2. ncbi Microtubules regulate migratory polarity through Rho/ROCK signaling in T cells
    Aya Takesono
    University College London, Department of Biochemistry and Molecular Biology and Ludwig Institute for Cancer Research, London, United Kingdom
    PLoS ONE 5:e8774. 2010
    ..Microtubules (MTs) are required for persistent migration and chemotaxis, but how they affect cell polarity is not known...
  3. ncbi Signal-regulated activation of serum response factor is mediated by changes in actin dynamics
    A Sotiropoulos
    Transcription Laboratory, Imperial Cancer Research Fund Laboratories, London, United Kingdom
    Cell 98:159-69. 1999
    ..Actin treadmilling provides a convergence point for both serum- and LIM kinase-1-induced signaling to SRF...
  4. ncbi Phosphorylation of Neurogenin2 specifies the migration properties and the dendritic morphology of pyramidal neurons in the neocortex
    Randal Hand
    Department of Phamacology, Neuroscience Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Neuron 48:45-62. 2005
    ....
  5. ncbi Tissue-specific GATA factors are transcriptional effectors of the small GTPase RhoA
    F Charron
    , , , , Canada H2W 1R7
    Genes Dev 15:2702-19. 2001
    ..The identification of transcription factor GATA-4 as a RhoA mediator in sarcomere reorganization and cardiac gene regulation provides a link between RhoA effects on transcription and cell remodeling...
  6. ncbi Reassembly of contractile actin cortex in cell blebs
    Guillaume T Charras
    Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA
    J Cell Biol 175:477-90. 2006
    ..Ezrin played no role in actin nucleation, but was essential for tethering the membrane to the cortex. The Rho pathway was important for cortex assembly in blebs...
  7. ncbi Muscle-specific signaling mechanism that links actin dynamics to serum response factor
    Koichiro Kuwahara
    Department of Molecular Biology, University of Texas Southwestern Medical Center at Dallas, 6000 Harry Hines Blvd, Dallas, TX 75390, USA
    Mol Cell Biol 25:3173-81. 2005
    ....
  8. ncbi Tumor suppressor p53 restricts Ras stimulation of RhoA and cancer cell motility
    Mingxuan Xia
    Department of Biomedical Genetics and James P Wilmot Cancer Center, University of Rochester Medical Center, Rochester, New York 14642, USA
    Nat Struct Mol Biol 14:215-23. 2007
    ..Our data suggest that p53 suppresses cancer progression to malignancy by modulating the quality of Ras signaling...
  9. ncbi Direct interaction of focal adhesion kinase with p190RhoGEF
    Jinbin Zhai
    Division of Neuropathology, University of Pennsylvania School of Medicine, Philadelphia 19104, USA
    J Biol Chem 278:24865-73. 2003
    ....
  10. ncbi Cyclic GMP-dependent protein kinase signaling pathway inhibits RhoA-induced Ca2+ sensitization of contraction in vascular smooth muscle
    V Sauzeau
    Laboratoire de Physiologie Cellulaire et Moleculaire, INSERM U 533, Faculte des Sciences, 44322 Nantes, France
    J Biol Chem 275:21722-9. 2000
    ....
  11. ncbi A role for Cdc42 in macrophage chemotaxis
    W E Allen
    Muscle and Motility Research Centre, Randall Institute, King s College London WC2B 5RL, UK
    J Cell Biol 141:1147-57. 1998
    ..We conclude that Rho and Rac are required for the process of cell migration, whereas Cdc42 is required for cells to respond to a gradient of CSF-1 but is not essential for cell locomotion...
  12. ncbi Therapeutic potential of RhoA/Rho kinase inhibitors in pulmonary hypertension
    M Oka
    Department of Pharmacology and Center for Lung Biology, University of South Alabama, College of Medicine, Mobile, AL 36688, USA
    Br J Pharmacol 155:444-54. 2008
    ..We propose that ROCK inhibitors and other drugs that inhibit this pathway might be useful in the treatment of various forms of PH...
  13. ncbi The PRK2 kinase is a potential effector target of both Rho and Rac GTPases and regulates actin cytoskeletal organization
    S Vincent
    Massachusetts General Hospital Cancer Center and Harvard Medical School, Charlestown 02129, USA
    Mol Cell Biol 17:2247-56. 1997
    ..These results indicate that p140 may be a shared kinase target of both Rho and Rac GTPases that mediates their effects on rearrangements of the actin cytoskeleton...
  14. ncbi Direct activation of RhoA by reactive oxygen species requires a redox-sensitive motif
    Amir Aghajanian
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Lineberger Comprehensive Cancer Center, Chapel Hill, North Carolina, United States of America
    PLoS ONE 4:e8045. 2009
    ..We hypothesized that this redox-based mechanism occurs in cells and affects cytoskeletal dynamics, and in this report we conclude this is indeed a novel mechanism of regulating the GTPase RhoA...
  15. ncbi Leukemia-associated Rho guanine nucleotide exchange factor, a Dbl family protein found mutated in leukemia, causes transformation by activation of RhoA
    G W Reuther
    Department of Pharmacology, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7295, USA
    J Biol Chem 276:27145-51. 2001
    ..These data demonstrate that LARG is the first functional Dbl protein mutated in cancer and indicate LARG-mediated activation of RhoA may play a role in the development of human leukemias...
  16. ncbi RhoA prenylation is required for promotion of cell growth and transformation and cytoskeleton organization but not for induction of serum response element transcription
    C Allal
    Oncologie Cellulaire et Moléculaire, EA 2048 Université Paul Sabatier, Centre de Lutte Contre le Cancer Claudius Regaud, 20 24 rue du Pont saint Pierre, 31052 Toulouse Cedex, France
    J Biol Chem 275:31001-8. 2000
    ..These results demonstrate that RhoA geranylgeranylation is required for its biological activity and that the nature of the lipid modification is not critical...
  17. ncbi Apocynin-induced vasodilation involves Rho kinase inhibition but not NADPH oxidase inhibition
    Torsten Schlüter
    Department of Physiology, University of Greifswald, Greifswalder Street 11C, D17495 Greifswald, Karlsburg, Germany
    Cardiovasc Res 80:271-9. 2008
    ..Further experiments were performed in normotensive rats and in NADPH oxidase subunit knock-out mice to test if apocynin-induced vasodilation depends on NADPH oxidase inhibition at all...
  18. ncbi Differential effect of Rac and Cdc42 on p38 kinase activity and cell cycle progression of nonadherent primary mouse fibroblasts
    A Philips
    Institut de Genetique Moleculaire, CNRS, UMR 5535, IFR 24, 1919 Route de Mende, 34293 Montpellier Cedex 5, France
    J Biol Chem 275:5911-7. 2000
    ..This demonstrates a novel function for Cdc42, distinct from that of Rac1, in the control of cell proliferation...
  19. ncbi The structural basis of Rho effector recognition revealed by the crystal structure of human RhoA complexed with the effector domain of PKN/PRK1
    R Maesaki
    Division of Structural Biology, Nara Institute of Science and Technology, Japan
    Mol Cell 4:793-803. 1999
    ..Sequence analysis based on the structure suggests that the ACC finger fold is widespread in Rho effector proteins...
  20. ncbi Rho GTPases in human breast tumours: expression and mutation analyses and correlation with clinical parameters
    G Fritz
    Institute of Toxicology, Division of Applied Toxicology, University of Mainz, Obere Zahlbacher Str 67, D 55131 Mainz, Germany
    Br J Cancer 87:635-44. 2002
    ....
  21. ncbi Guanine nucleotide exchange factor-H1 regulates cell migration via localized activation of RhoA at the leading edge
    Perihan Nalbant
    Departments of Immunology and Microbial Science, and Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Mol Biol Cell 20:4070-82. 2009
    ..This study establishes GEF-H1 as a critical organizer of key structural and signaling components of cell migration through the localized regulation of RhoA activity at the cell leading edge...
  22. ncbi RhoA sustains integrin alpha IIbbeta 3 adhesion contacts under high shear
    Simone M Schoenwaelder
    Department of Medicine, Australian Centre for Blood Diseases, Monash University, Box Hill Hospital, Arnold St, Box Hill, Victoria 3128, Australia
    J Biol Chem 277:14738-46. 2002
    ..These studies define a critical role for RhoA in regulating the stability of integrin alpha(IIb)beta(3) adhesion contacts under conditions of high shear stress...
  23. ncbi A PDZ-binding motif as a critical determinant of Rho guanine exchange factor function and cell phenotype
    Miaoliang Liu
    Angiogenesis Research Centre and Section of Cardiology, Dartmouth Medical School, Lebanon, NH 03756, USA
    Mol Biol Cell 17:1880-7. 2006
    ..We found that the activity level of JNK, which mediates transcriptional regulation downstream of RhoA, is elevated in a Syx2-dependent manner in these cells, possibly contributing to their tumorigenicity...
  24. ncbi Keratinocyte differentiation is regulated by the Rho and ROCK signaling pathway
    Rachel McMullan
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom
    Curr Biol 13:2185-9. 2003
    ..Thus, these results indicate that ROCK plays a critical role in regulating the balance between proliferation and differentiation in human keratinocytes...
  25. ncbi A role for the Rho-p160 Rho coiled-coil kinase axis in the chemokine stromal cell-derived factor-1alpha-induced lymphocyte actomyosin and microtubular organization and chemotaxis
    Miguel Vicente-Manzanares
    Servicio de Inmunologia, Hospital de la Princesa, Universidad Autonoma de Madrid, C Diego de Leon, 62, E 28006 Madrid, Spain
    J Immunol 168:400-10. 2002
    ..Our results indicate that the Rho-p160ROCK axis plays a pivotal role in the control of the cell shape as a step before lymphocyte migration toward a chemotactic gradient...
  26. ncbi Rho kinase inhibition by fasudil ameliorates diabetes-induced microvascular damage
    Ryoichi Arita
    Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan
    Diabetes 58:215-26. 2009
    ..Involvement of the Rho/Rho kinase (ROCK) pathway in diabetic microvasculopathy and therapeutic potential of fasudil, a selective ROCK inhibitor, are investigated...
  27. ncbi The Na+-H+ exchanger-1 induces cytoskeletal changes involving reciprocal RhoA and Rac1 signaling, resulting in motility and invasion in MDA-MB-435 cells
    Angelo Paradiso
    Laboratory of Clinical and Experimental Oncology, Oncology Institute of Bari, Bari, Italy
    Breast Cancer Res 6:R616-28. 2004
    ....
  28. ncbi Why three Rho proteins? RhoA, RhoB, RhoC, and cell motility
    Ann P Wheeler
    Laboratory of Cellular Molecular Biology, Ludwig Institute for Cancer Research, London W1W 7BS, United Kingdom
    Exp Cell Res 301:43-9. 2004
    ..In cancer cells, the expression and activity of RhoA, B, and C is altered in different ways. Together, this evidence suggests that although the 3 isoforms of Rho are structurally highly homologous, they have different cellular functions...
  29. ncbi Rho and vascular disease
    Barbara E Rolfe
    Centre for Research in Vascular Biology, School of Biomedical Sciences, University of Queensland, QLD 4072, Australia
    Atherosclerosis 183:1-16. 2005
    ..Further identification of the mechanisms by which these proteins and their effectors act in the vasculature should lead to therapies that specifically target only the adverse effects of Rho signalling...
  30. ncbi GEF-H1 modulates localized RhoA activation during cytokinesis under the control of mitotic kinases
    Jörg Birkenfeld
    Departments of Immunology and Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Dev Cell 12:699-712. 2007
    ..Our results identify a GEF-H1-dependent mechanism to modulate localized RhoA activation during cytokinesis under the control of mitotic kinases...
  31. ncbi Redox regulation of RhoA
    Jongyun Heo
    Department of Biochemistry and Biophysics, The University of North Carolina, 530 Mary Ellen Jones Building, Chapel Hill, North Carolina 27599-7260, USA
    Biochemistry 45:14481-9. 2006
    ..Thus, in addition to redox agents, platinated-chemotherapeutic agents and arsenic complexes may modulate the activity of GTPases containing the GXXXCGK(S/T)C motif (i.e., RhoA and RhoB)...
  32. ncbi ROCK controls matrix synthesis in vascular smooth muscle cells: coupling vasoconstriction to vascular remodeling
    Rene Chapados
    Children's Hospital of Philadelphia, PA, USA
    Circ Res 99:837-44. 2006
    ..Finally, we showed that inhibition of ROCK activity suppresses SMC TN-C expression and disease progression in hypertensive rat PAs. Thus, in addition to its role in regulating vasoconstriction, ROCK also controls matrix production...
  33. ncbi RhoE binds to ROCK I and inhibits downstream signaling
    Kirsi Riento
    Ludwig Institute for Cancer Research, Royal Free and University College School of Medicine, London, United Kingdom
    Mol Cell Biol 23:4219-29. 2003
    ..These data suggest that RhoE induces stress fiber disassembly by directly binding ROCK I and inhibiting it from phosphorylating downstream targets...
  34. ncbi Ablation of p120-catenin enhances invasion and metastasis of human lung cancer cells
    Yang Liu
    Department of Pathology, College of Basic Medical Sciences, China Medical University, Heping District, Shenyang City, Liaoning Province, China
    Cancer Sci 100:441-8. 2009
    ....
  35. ncbi Vertebrate development requires ARVCF and p120 catenins and their interplay with RhoA and Rac
    Xiang Fang
    Department of Biochemistry and Molecular Biology, Box 117, University of Texas M D Anderson Cancer Center, 1515 Holcombe Blvd, Houston, Texas 77030 4095, USA
    J Cell Biol 165:87-98. 2004
    ..This work indicates the required functions of both p120 and ARVCF in vertebrate embryogenesis and their shared functional interplay with RhoA, Rac, and cadherin in a developmental context...
  36. ncbi Tech: a RhoA GEF selectively expressed in hippocampal and cortical neurons
    Ruth Marx
    Department of Neuroscience and Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    J Neurochem 92:850-8. 2005
    ..As Tech protein levels increase during post-natal development and remain at peak levels into adulthood, these results indicate that Tech regulates RhoA signaling pathways in developing and mature forebrain neurons...
  37. ncbi Roles of p-ERM and Rho-ROCK signaling in lymphocyte polarity and uropod formation
    Jong-Hwan Lee
    Center for Molecular Biology and Genetics, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan
    J Cell Biol 167:327-37. 2004
    ..Thus, phosphorylated ERM has a potential function in establishing plasma membrane "posteriority" in the induction of the uropod in T lymphocytes...
  38. ncbi The gene for a new brain specific RhoA exchange factor maps to the highly unstable chromosomal region 1p36.2-1p36.3
    M De Toledo
    , CNRS IFR24-UPR 1086, 1919 Route de Mende, 34293 Montpellier Cedex 5, France
    Oncogene 20:7307-17. 2001
    ..GEF720 is also a new candidate gene involved in the progression of neuroblastoma and developmental abnormalities associated with rearrangements in the 1p36 chromosomal region...
  39. ncbi KDR stimulates endothelial cell migration through heterotrimeric G protein Gq/11-mediated activation of a small GTPase RhoA
    Huiyan Zeng
    Department of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA
    J Biol Chem 277:46791-8. 2002
    ..Taken together, our results indicate that KDR stimulates endothelial cell migration through a heterotrimeric G protein Gq/11 and Gbetagamma-mediated RhoA pathway...
  40. ncbi Extension, retraction and contraction in the formation of a dendritic cell dendrite: distinct roles for Rho GTPases
    Claire A Swetman
    Department of Immunology and Molecular Pathology, University College London, London, GB
    Eur J Immunol 32:2074-83. 2002
    ..DC therefore coordinate adhesion and protrusion to perform a specialized process of cellular morphogenesis, which differentiates these cells from all other cells of the immune system and may contribute to their distinctive function...
  41. ncbi Rho and ROCK signaling in VEGF-induced microvascular endothelial hyperpermeability
    Hengrui Sun
    Department of Surgery, University of California at Davis School of Medicine, Sacramento, California, USA
    Microcirculation 13:237-47. 2006
    ..They also investigated myosin light chain (MLC) phosphorylation and actin polymerization, two well-characterized targets of the Rho-ROCK pathway that are implicated in the regulation of endothelial barrier function...
  42. ncbi Rho family GTPases bind to phosphoinositide kinases
    K F Tolias
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA
    J Biol Chem 270:17656-9. 1995
    ....
  43. ncbi Nogo-A and myelin-associated glycoprotein mediate neurite growth inhibition by antagonistic regulation of RhoA and Rac1
    Barbara Niederost
    Institute of Medical Chemistry and Biochemistry, Leopold Franzens University of Innsbruck, A 6020 Innsbruck, Austria
    J Neurosci 22:10368-76. 2002
    ..Furthermore, we show that the recently cloned receptor for Nogo-66 and MAG, NgR, is not necessary for either NiG- or MAG-induced RhoA activation...
  44. ncbi Smooth muscle differentiation marker gene expression is regulated by RhoA-mediated actin polymerization
    C P Mack
    Department of Molecular Physiology and Biological Physics, University of Virginia Medical School, Charlottesville, Virginia 22908, USA
    J Biol Chem 276:341-7. 2001
    ..The results also suggest that RhoA signaling may serve as a convergence point for the multiple signaling pathways that regulate SMC differentiation...
  45. ncbi Endotoxin inhibits intestinal epithelial restitution through activation of Rho-GTPase and increased focal adhesions
    Selma Cetin
    Division of Pediatric Surgery, Department of Surgery, Children s Hospital of Pittsburgh and University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    J Biol Chem 279:24592-600. 2004
    ..We therefore conclude that endotoxin inhibits enterocyte migration through a RhoA-dependent increase in focal adhesions and enhanced cell adhesiveness, which may participate in the impaired restitution observed in experimental NEC...
  46. ncbi Activation of RhoA and inhibition of myosin phosphatase as important components in hypertension in vascular smooth muscle
    Tetsuya Seko
    First Department of Internal Medicine, Mie University School of Medicine, 2 174 Edobashi, Tsu, Mie 514 8507, Japan
    Circ Res 92:411-8. 2003
    ..These results suggest that independent of the cause of hypertension, a common point in downstream signaling and a critical component of hypertension is activation of RhoA and subsequent activation of Rho-kinase...
  47. ncbi Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho-kinase)
    K Kimura
    Division of Signal Transduction, Nara Institute of Science and Technology, Ikoma 630 01, Japan
    Science 273:245-8. 1996
    ..Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase...
  48. ncbi T-cell integrins: more than just sticking points
    Nancy Hogg
    Leukocyte Adhesion Laboratory, Cancer Research UK London Research Institute, London WC2A 3PX, UK
    J Cell Sci 116:4695-705. 2003
    ..Another important signalling pathway acts through CasL/Crk, which might regulate the activity of the GTPases Rac and Rap1 that have important roles in T-cell migration...
  49. ncbi The p160 RhoA-binding kinase ROK alpha is a member of a kinase family and is involved in the reorganization of the cytoskeleton
    T Leung
    Glaxo IMCB Group, Institute of Molecular and Cell Biology, National University of Singapore, Singapore
    Mol Cell Biol 16:5313-27. 1996
    ....
  50. ncbi Modulation of RhoA-Rho kinase-mediated Ca2+ sensitization of rabbit myometrium during pregnancy - role of Rnd3
    C Cario-Toumaniantz
    INSERM U-533, , 44322 Nantes, France
    J Physiol 552:403-13. 2003
    ..The RhoA-Rho kinase signalling pathway and its regulators might thus represent potential targets for the development of new treatments for pre-term labour...
  51. ncbi Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment
    Rowena McBeath
    The Cellular and Molecular Medicine Program, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Dev Cell 6:483-95. 2004
    ..These studies demonstrate that mechanical cues experienced in developmental and adult contexts, embodied by cell shape, cytoskeletal tension, and RhoA signaling, are integral to the commitment of stem cell fate...
  52. ncbi Protein kinase N (PKN) and PKN-related protein rhophilin as targets of small GTPase Rho
    G Watanabe
    Department of Pharmacology, Kyoto University Faculty of Medicine, Japan
    Science 271:645-8. 1996
    ..This study indicates that a serine-threonine protein kinase is a Rho effector and presents an amino acid sequence motif for binding to GTP-Rho that may be shared by a family of Rho target proteins...
  53. ncbi Coronary smooth muscle differentiation from proepicardial cells requires rhoA-mediated actin reorganization and p160 rho-kinase activity
    J Lu
    Department of Pathology, Center for Cardiovascular Development, Graduate Program in Cardiovascular Sciences, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
    Dev Biol 240:404-18. 2001
    ....
  54. ncbi The Ras-related GTPase Rac1 binds tubulin
    A Best
    Department of Neurochemistry, Institute of Neurology, 1, Wakefield St, London WC1N 1PJ, United Kingdom
    J Biol Chem 271:3756-62. 1996
    ..These results suggest that the Rac1-tubulin interaction may play a role in Rac1 function...
  55. ncbi The insert region of RhoA is essential for Rho kinase activation and cellular transformation
    H Zong
    Department of Biochemistry and Molecular Biology and Walther Oncology Center, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA
    Mol Cell Biol 21:5287-98. 2001
    ..In conclusion, we have demonstrated that the insert region of RhoA is required for Rho kinase activation but not for binding and that this kinase activity is required to induce morphologic transformation of NIH 3T3 cells...
  56. ncbi The molecular requirements for cytokinesis
    Michael Glotzer
    Research Institute of Molecular Pathology, Dr Bohrgasse 7, A 1030 Vienna, Austria
    Science 307:1735-9. 2005
    ..Cytokinesis involves additional proteins, but they, or their requirement in cytokinesis, are not conserved among animal cells...
  57. ncbi Fine regulation of RhoA and Rock is required for skeletal muscle differentiation
    Loriana Castellani
    Istituto di Biologia Cellulare, Consiglio Nazionale delle Ricerche, 00016 Monterotondo Scalo RM, Italy
    J Biol Chem 281:15249-57. 2006
    ..Finally, we have reported that Rock acts independently from RhoA in preventing myoblast exit from the cell cycle and commitment to differentiation and may receive signaling inputs from Raf-1 kinase...
  58. ncbi Mutant p53 induces the GEF-H1 oncogene, a guanine nucleotide exchange factor-H1 for RhoA, resulting in accelerated cell proliferation in tumor cells
    Shinji Mizuarai
    Functional Genomics, Banyu Tsukuba Research Institute, Merck Research Laboratory, Tsukuba, Ibaraki, Japan
    Cancer Res 66:6319-26. 2006
    ..These results suggest that increased GEF-H1 expression contributes to the tumor progression phenotype associated with the p53 mutation...
  59. ncbi Podocalyxin activates RhoA and induces actin reorganization through NHERF1 and Ezrin in MDCK cells
    Sandra Schmieder
    Department of Cellular and Molecular Medicine, George Palade Laboratories Room 210, 9500 Gilman Drive, La Jolla, CA 92093-0651, USA
    J Am Soc Nephrol 15:2289-98. 2004
    ..PC activates RhoA through NHERF and ezrin, leading to redistribution of actin filaments. These results suggest that in podocytes, PC may also regulate foot process architecture through RhoA...
  60. ncbi Localized zones of Rho and Rac activities drive initiation and expansion of epithelial cell-cell adhesion
    Soichiro Yamada
    Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, USA
    J Cell Biol 178:517-27. 2007
    ..We show that zones of Rac1 and lamellipodia activity and of RhoA and actomyosin contractility are restricted to the periphery of contacting membranes and together drive initiation, expansion, and completion of cell-cell adhesion...
  61. ncbi Rho GTPases are over-expressed in human tumors
    G Fritz
    Institute of Toxicology, Division of Applied Toxicology, University of Mainz, Germany
    Int J Cancer 81:682-7. 1999
    ..Overall, increase in the amount of Rho GTPases, in particular RhoA, appears to be a frequent event in different types of human tumors. This supports the view that Rho GTPases are involved in human carcinogenesis...
  62. ncbi Rnd proteins function as RhoA antagonists by activating p190 RhoGAP
    Krister Wennerberg
    Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Curr Biol 13:1106-15. 2003
    ..However, effector molecules of Rnd proteins with such properties have not been identified...
  63. ncbi A novel serine/threonine kinase binding the Ras-related RhoA GTPase which translocates the kinase to peripheral membranes
    T Leung
    Glaxo IMCB Group, Institute of Molecular and Cell Biology, National University of Singapore, Kent Ridge, Singapore
    J Biol Chem 270:29051-4. 1995
    ..These results are consistent with a role for the kinase ROK alpha as an effector for RhoA...
  64. ncbi Role of RhoA, mDia, and ROCK in cell shape-dependent control of the Skp2-p27kip1 pathway and the G1/S transition
    Akiko Mammoto
    Vascular Biology Program, Department of Pathology, Children s Hospital Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 279:26323-30. 2004
    ....
  65. ncbi Extra-nuclear signaling of progesterone receptor to breast cancer cell movement and invasion through the actin cytoskeleton
    Xiao Dong Fu
    Molecular and Cellular Gynecological Endocrinology Laboratory, Department of Reproductive Medicine and Child Development, University of Pisa, Pisa, Italy
    PLoS ONE 3:e2790. 2008
    ..These results provide original mechanistic explanations for the effects of progestins on breast cancer progression and highlight potential targets to treat endocrine-sensitive breast cancers...
  66. ncbi The Rho family GTPases RhoA, Rac1, and CDC42Hs regulate transcriptional activation by SRF
    C S Hill
    Transcription Laboratory, Imperial Cancer Research Fund Laboratories, London, England
    Cell 81:1159-70. 1995
    ..Functional Rho is required for regulated activity of the c-fos promoter. These results establish SRF as a nuclear target of a novel Rho-mediated signaling pathway...
  67. ncbi Rho mediates calcium-dependent activation of p38alpha and subsequent excitotoxic cell death
    Maria M Semenova
    Department of Neurobiology, A I Virtanen Institute, University of Kuopio, Kuopio FIN 70211, Finland
    Nat Neurosci 10:436-43. 2007
    ..Therefore, Rho is an essential component of the excitotoxic cell death pathway...
  68. ncbi Rho activation patterns after spinal cord injury and the role of activated Rho in apoptosis in the central nervous system
    Catherine I Dubreuil
    Departement de pathologie et biologie cellulaire, Universite de Montreal, Montreal, QC H3T 1J4, Canada
    J Cell Biol 162:233-43. 2003
    ..Experiments with p75NTR-null mutant mice showed that immediate Rho activation after SCI is p75NTR dependent. Our results indicate that blocking overactivation of Rho after SCI protects cells from p75NTR-dependent apoptosis...
  69. ncbi Deleted in liver cancer 2 (DLC2) suppresses cell transformation by means of inhibition of RhoA activity
    Thomas Ho Yin Leung
    Department of Pathology, SH Ho Foundation Research Laboratories and Hong Kong Jockey Club Clinical Research Centre, Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong
    Proc Natl Acad Sci U S A 102:15207-12. 2005
    ..These results suggest that DLC2 exhibits its tumor suppressor functions in vivo as a GAP specific for RhoA, exerting its effects in suppression of cytoskeleton reorganization, cell growth, cell migration, and transformation...
  70. ncbi Aspergillus nidulans RhoA is involved in polar growth, branching, and cell wall synthesis
    Gretel M Guest
    Department of Plant Biology, University of Georgia, Athens, GA 30602, USA
    Fungal Genet Biol 41:13-22. 2004
    ..Our results suggest that rhoA plays a role in polarity, proper branching pattern, and cell wall deposition...
  71. ncbi RhoB, not RhoA, represses the transcription of the transforming growth factor beta type II receptor by a mechanism involving activator protein 1
    Jalila Adnane
    Drug Discovery Program, H Lee Moffitt Cancer Center and Research Institute, Department of Oncology, University of South Florida, Tampa, Florida 33612, USA
    J Biol Chem 277:8500-7. 2002
    ..Altogether, these results identify a mechanism by which RhoB antagonizes TGF-beta action through transcriptional down-regulation of AP1 in T beta R-II promoter...
  72. ncbi RhoA/ROCK activation by growth hormone abrogates p300/histone deacetylase 6 repression of Stat5-mediated transcription
    Ling Ling
    Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609, Republic of Singapore
    J Biol Chem 279:32737-50. 2004
    ..We have therefore provided a novel mechanism by which a Ras-like small GTPase, RhoA, can regulate Stat5-mediated transcription...
  73. ncbi Increased RhoA translocation in aorta of diabetic rats
    Jiping Tang
    Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, MS 39216, USA
    Acta Pharmacol Sin 27:543-8. 2006
    ..To analyze RhoA expression and activation in the aorta of diabetic rats...
  74. ncbi Cytokinesis: welcome to the Rho zone
    Alisa Piekny
    Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637, USA
    Trends Cell Biol 15:651-8. 2005
    ..Advances in detecting the active pool of RhoA have allowed insights into the mechanisms and the molecules that promote the accumulation of active RhoA at the correct time and place in the cell...
  75. ncbi RhoA/Rho kinase up-regulate Bax to activate a mitochondrial death pathway and induce cardiomyocyte apoptosis
    Dominic P Del Re
    Department of Pharmacology and Biomedical Sciences Graduate Program, University of California San Diego, La Jolla, California 92093, USA
    J Biol Chem 282:8069-78. 2007
    ..The dominant negative p53 also prevents RhoA-induced apoptosis. We conclude that RhoA/Rho kinase activation up-regulates Bax through p53 to induce a mitochondrial death pathway and cardiomyocyte apoptosis...
  76. ncbi Loss of p53 promotes RhoA-ROCK-dependent cell migration and invasion in 3D matrices
    Gilles Gadea
    Centre de Recherche en Biochimie Macromoleculaire, Centre National de la Recherche Scientifique, Universite Mixte de Recherche 5237, Institut Federatif de Recherche 122, F 34293 Montpellier, Cedex 5, France
    J Cell Biol 178:23-30. 2007
    ..A similar p53-mediated transition is observed in melanoma A375P cancer cells. Our data suggest that genetic alterations of p53 in tumors are sufficient to promote motility and invasion, thereby contributing to metastasis...
  77. ncbi Asymmetric membrane ganglioside sialidase activity specifies axonal fate
    Jorge Santos Da Silva
    Cavalieri Ottolenghi Scientific Institute, University of Turin, 10043 Orbassano, Turin, Italy
    Nat Neurosci 8:606-15. 2005
    ..Thus, spatial restriction of an actin-regulating molecular machinery, in this case a membrane enzymatic activity, before polarization is enough to determine axonal fate...
  78. ncbi Pharmacological inhibition of RhoA signaling prevents connective tissue growth factor induction in endothelial cells exposed to non-uniform shear stress
    Iwona Cicha
    Medical Clinic 2, University of Erlangen Nuremberg, Erlangen, Germany
    Atherosclerosis 196:136-45. 2008
    ..Interference with shear stress-induced protein expression may inhibit endothelial dysfunction in atheroprone vessel regions...
  79. ncbi The RhoA transcriptional program in pre-T cells
    M Mullin
    Samuel Lunenfeld Research Institute, 600 University Avenue, Toronto, Ontario, Canada
    FEBS Lett 581:4309-17. 2007
    ..Transcriptional profiling reveals both common and unique gene targets for RhoA and the pre-TCR indicating that RhoA participates in the pre-TCR induced transcriptional program but also mediates pre-TCR independent gene transcription...
  80. ncbi Smurf1 regulates tumor cell plasticity and motility through degradation of RhoA leading to localized inhibition of contractility
    Erik Sahai
    Centre National de la Recherche Scientifique, UMR 6543, Centre Antoine Lacassagne, Nice 06189, France
    J Cell Biol 176:35-42. 2007
    ..Our results suggest that Smurf1 is a pivotal regulator of tumor cell movement through its regulation of RhoA signaling...
  81. ncbi F11L-mediated inhibition of RhoA-mDia signaling stimulates microtubule dynamics during vaccinia virus infection
    Yoshiki Arakawa
    Cell Motility Laboratory, Cancer Research UK, London Research Institute, 44 Lincoln s Inn Fields, London WC2A 3PX, UK
    Cell Host Microbe 1:213-26. 2007
    ..They do, however, require F11L-mediated inhibition of signaling via the GTPase RhoA and its effector, mDia. We suggest that increases in peripheral microtubule dynamics and cortical targeting contribute to enhanced virus release...
  82. ncbi beta-Arrestin 1 and Galphaq/11 coordinately activate RhoA and stress fiber formation following receptor stimulation
    William G Barnes
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 280:8041-50. 2005
    ....
  83. ncbi Rac and Rho play opposing roles in the regulation of hypoxia/reoxygenation-induced permeability changes in pulmonary artery endothelial cells
    Beata Wojciak-Stothard
    British Heart Foundation Laboratories, Department of Medicine, University College London, 5 University St, WC1 E6JJ London, UK
    Am J Physiol Lung Cell Mol Physiol 288:L749-60. 2005
    ..Our results show that Rho GTPases act as mediators coupling cellular redox state to endothelial function...
  84. ncbi Cell type-specific regulation of RhoA activity during cytokinesis
    Hisayoshi Yoshizaki
    Department of Tumor Virology, Research Institute for Microbial Diseases, Osaka University, Yamadaoka, Suita Shi, Osaka 565 0871, Japan
    J Biol Chem 279:44756-62. 2004
    ....
  85. ncbi RhoA signaling in phorbol ester-induced apoptosis
    Zee Fen Chang
    Institute of Biochemistry and Molecular Biology, College of Medicine, National Taiwan University, No 1, Section 1, Jen Ai Road, Taipei, Taiwan, ROC
    J Biomed Sci 13:173-80. 2006
    ..Thus, a cytoskeleton-regulated RhoA signaling cooperates with PKC activation constitutes a cellular context to determine the cell fate in response to phorbol ester stimulation...
  86. ncbi Identification of a putative target for Rho as the serine-threonine kinase protein kinase N
    M Amano
    Division of Signal Transduction, Nara Institute of Science and Technology, Ikoma, Japan
    Science 271:648-50. 1996
    ..PKN was phosphorylated in Swiss 3T3 cells stimulated with LPA, and this phosphorylation was blocked by treatment of cells with botulinum C3 exoenzyme. Activation of Rho may be linked directly to a serine-threonine kinase pathway...
  87. ncbi Characterization of p190RhoGEF, a RhoA-specific guanine nucleotide exchange factor that interacts with microtubules
    F P van Horck
    Division of Cellular Biochemistry, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    J Biol Chem 276:4948-56. 2001
    ....
  88. ncbi Involvement of the small GTPases XRhoA and XRnd1 in cell adhesion and head formation in early Xenopus development
    K Wünnenberg-Stapleton
    Department of Developmental and Cell Biology, and Developmental Biology Center, University of California, Irvine, CA 92697, USA
    Development 126:5339-51. 1999
    ..Thus, XRhoA may have a dual role in the embryo by regulating cell adhesion properties and pattern formation...
  89. ncbi Essential roles of Drosophila RhoA in the regulation of neuroblast proliferation and dendritic but not axonal morphogenesis
    T Lee
    Department of Biological Sciences, Stanford University, California 94305, USA
    Neuron 25:307-16. 2000
    ..Thus, RhoA is an important regulator of dendritic morphogenesis, while distinct mechanisms are used for axonal morphogenesis...
  90. ncbi Activation of Rhoa and ROCK are essential for detachment of migrating leukocytes
    J Alblas
    UMC Utrecht, Department of Pulmonary Diseases, 3584 CX Utrecht, The Netherlands
    Mol Biol Cell 12:2137-45. 2001
    ..From these results we conclude that activation of the RhoA-ROCK pathway is essential for detachment of migratory leukocytes...
  91. ncbi The Amot/Patj/Syx signaling complex spatially controls RhoA GTPase activity in migrating endothelial cells
    Mira Ernkvist
    Department of Oncology and Pathology, Cancer Centrum Karolinska, Karolinska Institutet, Karolinska Hospital, Stockholm, Sweden
    Blood 113:244-53. 2009
    ..Taken together, our results indicate that the directional migration of capillaries in the embryo is governed by the Amot:Patj/Mupp1:Syx signaling that controls local GTPase activity...
  92. ncbi RhoA and ROCK promote migration by limiting membrane protrusions
    Rebecca A Worthylake
    Department of Cell and Developmental Biology, Lineberger Comprehensive Cancer Center and Comprehensive Center for Inflammatory Disorders, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 278:13578-84. 2003
    ..Together, our results show that RhoA/ROCK signaling promotes migration by restricting integrin activity and membrane protrusions to the leading edge...
  93. ncbi Novel molecular insights into RhoA GTPase-induced resistance to aqueous humor outflow through the trabecular meshwork
    Min Zhang
    Dept of Ophthalmology, Duke Univ School of Medicine, 2351 Erwin Rd, Durham, NC 27710, USA
    Am J Physiol Cell Physiol 295:C1057-70. 2008
    ....
  94. ncbi Rho GTPases control migration and polarization of adhesion molecules and cytoskeletal ERM components in T lymphocytes
    M A del Pozo
    Servicio de Inmunologia, Hospital de la Princesa, Universidad Autonoma de Madrid, Madrid, Spain
    Eur J Immunol 29:3609-20. 1999
    ..Together, these data demonstrate that Rho GTPases regulate lymphocyte polarization and chemokine-induced migration, and underscore the key role of Cdc42 in lymphocyte directional migration...
  95. ncbi Regulation of inositol lipid kinases by Rho and Rac
    X D Ren
    Department of Vascular Biology, Scripps Research Institute, La Jolla, California 92037, USA
    Curr Opin Genet Dev 8:63-7. 1998
    ..Rho family GTPases also interact with phosphatidylinositol 3-kinase, though this kinase can be either upstream or downstream of the GTPases depending upon the system...
  96. ncbi Lysophosphatidic acid promotes phorbol-ester-induced apoptosis in TF-1 cells by interfering with adhesion
    J M Lai
    Institute of Biochemistry, College of Medicine, National Taiwan University, College of Medicine, No. 1 Jen-Ai Road, Section 1, Taipei, Taiwan, ROC
    Biochem J 359:227-33. 2001
    ....
  97. ncbi Mutation status of genes encoding RhoA, Rac1, and Cdc42 GTPases in a panel of invasive human colorectal and breast tumors
    S Rihet
    Laboratoire de Signalisation Intracellulaire et Oncogènes, Inserm U 528, Institut Curie, Paris, France
    J Cancer Res Clin Oncol 127:733-8. 2001
    ....
  98. ncbi Ca2+-dependent activation of Rho and Rho kinase in membrane depolarization-induced and receptor stimulation-induced vascular smooth muscle contraction
    Sotaro Sakurada
    Department of Physiology, Kanazawa University Graduate School of Medicine, Kanazawa, Ishikawa, Japan
    Circ Res 93:548-56. 2003
    ..Taken together, these results indicate the existence of the thus-far unrecognized Ca2+-dependent Rho stimulation mechanism in VSM. Excitatory receptor agonists are suggested to use this pathway for simulating Rho...
  99. ncbi Interaction of protein kinase C isozymes with Rho GTPases
    S J Slater
    Department of Pathology, Cell Biology and Anatomy, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Biochemistry 40:4437-45. 2001
    ..Such an interaction could result in significant cross-talk between the distinct pathways regulated by these two signaling elements...
  100. ncbi The G12/13-RhoA signaling pathway contributes to efficient lysophosphatidic acid-stimulated cell migration
    D Bian
    Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Oncogene 25:2234-44. 2006
    ..Our studies show that the G12/13-RhoA-ROCK signaling pathway mediates LPA-induced FAK autophosphorylation and contributes to LPA-stimulated cell migration...
  101. ncbi Epidermal growth factor stimulation of the ACK1/Dbl pathway in a Cdc42 and Grb2-dependent manner
    J Kato-Stankiewicz
    Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, 226 8501, Japan
    Biochem Biophys Res Commun 284:470-7. 2001
    ..These results provide evidence that ACK1 acts as a mediator of EGF signals to Rho family GTP-binding proteins through phosphorylation and activation of GEFs such as Dbl...

Research Grants147 found, 100 shown here

  1. Adrenergic activation of NHE requires PLD in CCL39 cells
    JOSEPH PROVOST; Fiscal Year: 2006
    ..All of the preliminary data was collected by undergraduates and the funding of this project would involve 15 to 20 undergraduates in meaningful research over the three-year term of the grant. ..
  2. Regulation of Rho Signaling by S-Nitrosothiols
    A Richard Whorton; Fiscal Year: 2010
    ..Results from our studies will provide insight into prevention and development of potential therapeutic strategies. ..
  3. Significance and Function of HGAL in Lymphoma
    Izidore Lossos; Fiscal Year: 2009
    ..These findings will not only elucidate the mechanisms of tumor dissemination but also will identify a new potential molecular target for novel therapeutic intervention. ..
  4. Significance and Function of HGAL in Lymphoma
    Izidore S Lossos; Fiscal Year: 2010
    ..These findings will not only elucidate the mechanisms of tumor dissemination but also will identify a new potential molecular target for novel therapeutic intervention. ..
  5. Significance and Function of HGAL in Lymphoma
    Izidore S Lossos; Fiscal Year: 2010
    ..These findings will not only elucidate the mechanisms of tumor dissemination but also will identify a new potential molecular target for novel therapeutic intervention. ..
  6. Endothelial Barrier Function Modulation by PKCdelta
    Elizabeth Harrington; Fiscal Year: 2004
    ..The elucidation of the molecular mechanisms by which PKCDELTA decreases endothelial monolayer permeability may lead to the development of therapeutic agents which are protective against endothelial barrier dysfunction. ..
  7. The Role of RhoA/Rho-kinase in Erectile Dysfunction in Type II Diabetes
    Liming Jin; Fiscal Year: 2007
    ..Understanding the molecular mechanisms of RhoA pathway regulating erectile function will provide a new therapeutic approach for treatment of ED. ..
  8. Control of Actomyosin by Microtubules
    William Bement; Fiscal Year: 2004
    ..Thus, the proposed work will provide important and novel insights into the means by which microtubules control actomyosin in vivo. ..
  9. Endothelial Barrier Function Modulation by PKCdelta
    Elizabeth Harrington; Fiscal Year: 2009
    ..We hope that identification of proteins important in maintaining a non-leaky state will assist in discovering a treatment for patients suffering from lung edema. ..
  10. Actin-microtubule cross-talk in transient cell arrays
    William Bement; Fiscal Year: 2007
    ..The successful completion of the proposed experiments will not only answer basic questions in biology, it will also provide critical insights into processes such as wound repair, cancer, birth defects and human infertility. ..
  11. Endothelial Barrier Function Modulation by PKCdelta
    Elizabeth Harrington; Fiscal Year: 2007
    ..We hope that identification of proteins important in maintaining a non-leaky state will assist in discovering a treatment for patients suffering from lung edema. ..
  12. Transient cytoskeletal arrays
    William Bement; Fiscal Year: 2010
    ..This work will provide cellular and molecular insights into these processes which will permit informed design of clinical interventions for these diseases. ..
  13. Transient cytoskeletal arrays
    William Bement; Fiscal Year: 2010
    ..This work will provide cellular and molecular insights into these processes which will permit informed design of clinical interventions for these diseases. ..
  14. FUNCTIONAL/MOLECULAR CONTROL OF ACTOMYOSIN--MICROTUBULE
    William Bement; Fiscal Year: 1999
    ..3) Cytoskeletal reorganization during cortical flow will be monitored by immunofluorescence and confocal microscopy. 4) Finally, he will determine the effect of MAPs on cortical flow by identifying Xenopus MAP(s). ..
  15. Olympus FV1000 Laser Scanning Confocal Microscope
    William Bement; Fiscal Year: 2007
    ..This information, in turn, will help guide future investigations of a number of normal and pathological human processes such as wound repair, nerve regeneration, cancer, and birth defects. ..
  16. Endothelial Barrier Function Modulation by PKCdelta
    Elizabeth O Harrington; Fiscal Year: 2010
    ..We hope that identification of proteins important in maintaining a non-leaky state will assist in discovering a treatment for patients suffering from lung edema. ..
  17. IGF-1, Oxidative Stress and Cardiovascular Aging
    Jun Ren; Fiscal Year: 2002
    ..Our long-term goal is to establish the causal link among IGF-1 deficiency, enhanced oxidative damage and ventricular dysfunction in the progression of cardiovascular aging so that hormonal or antioxidant therapy can be optimized...
  18. Ceruloplasmin Mediated Penile Endothelial Dysfunction in Type 1 Diabetes
    Kanchan Chitaley; Fiscal Year: 2007
    ..Relevance to Public Health: Treatment or prevention of ED in men with diabetes is necessary to improve life satisfaction and reduce the utilization of health care resources in this rapidly growing portion of the American population. ..
  19. CD44-P185HER2 INTERACTION IN OVARIAN CANCER PROGRESSION
    LILLY BOURGUIGNON; Fiscal Year: 2007
    ..abstract_text> ..
  20. FoxM1 regulates endothelial repair following lung vascular injury
    You Yang Zhao; Fiscal Year: 2010
    ..We will also examine following LPS-induced vascular injury the ultrastructural defects and the cellular nature of the leaks and repair failure of the pulmonary vasculature in mice with endothelial cell- restricted deficiency of FoxM1. ..
  21. Versatile scaffolds to visualize endogenous protein activation in living cells
    Klaus Hahn; Fiscal Year: 2009
    ..PAK, JNK, and ERK2 are each on a different, parallel pathway downstream of Src. The spatio-temporal regulation of this signaling network to produce different cellular responses will be examined. ..
  22. Shear Stress, AMPK, and Endothelial Functions
    John Y J Shyy; Fiscal Year: 2010
    ....
  23. CORE--Imaging and Physiology Core
    Richard Minshall; Fiscal Year: 2007
    ..Core D personnel, who have extensive training and experience in the described procedures, will provide the support and resources outlined below as well as the necessary training of personnel in all Projects. ..
  24. Regulation of Endothelial ICAM-1 and PMN-Mediated Lung Injury by Actin Dynamics
    Fabeha Fazal; Fiscal Year: 2010
    ....
  25. Caveolin-1 and NO Regulate PMN-mediated Increases in Vascular Permeability
    Richard Minshall; Fiscal Year: 2009
    ..Project Description Page 6 ..
  26. Entry of HHV-8 Into the Target Cells
    Bala Chandran; Fiscal Year: 2007
    ..Further understanding of HHV-8-entry, integrin-dependent cell signaling pathways, and their role in infection would eventually lead to the development of new anti-HHV-8 agents. ..
  27. Nitric Oxide-Mediated Pulmonary Vasodilation After Chronic Hypoxia
    Thomas Resta; Fiscal Year: 2007
    ..Findings from studies are expected to fundamentally advance our understanding of signal transduction mechanisms by which PKG regulates pulmonary vascular tone, as well as adaptive responses of the pulmonary circulation to CH. ..
  28. Regulation of Vascular Calcification in Diabetes
    XUENING CHEN; Fiscal Year: 2007
    ....
  29. NEUROHUMORAL CONTROL OF INTERNAL ANAL SPHINCTER
    Satish Rattan; Fiscal Year: 2005
    ..Information produced by these studies will permit an understanding of the pathophysiology that underlies disorders of the LAS, and provide a rationale for development of therapies to treat disorders of the IAS. ..
  30. NEUROHUMORAL CONTROL OF INTERNAL ANAL SPHINCTER
    Satish Rattan; Fiscal Year: 2001
    ..The proposed studies should provide important information leading to a better understanding of the pathophysiology and the treatment of anorectal disorders. ..
  31. ENVELOPE GLYCOPROTEINS OF HHV8
    Bala Chandran; Fiscal Year: 2002
    ..1(A and B) and gB will lead into an important in sight into the development of future control measures. ..
  32. ANTIGENS OF KAPOSIS SARCOMA ASSOCIATED HERPESVIRUS
    Bala Chandran; Fiscal Year: 2001
    ..These studies could be significant since if HHV-8 replication precedes the development of KS then this may have important implications in the assessment of therapeutic intervention of HHV-8 infection by anti-herpes viral drugs. ..
  33. Entry of HHV-8 Into the Target Cells
    Bala Chandran; Fiscal Year: 2009
    ..Further understanding of HHV-8-entry, integrin-dependent cell signaling pathways, and their role in infection would eventually lead to the development of new anti-HHV-8 agents. ..
  34. Antigens of Kaposi's sarcoma associated herpesvirus
    Bala Chandran; Fiscal Year: 2010
    ....