adp ribosylation factor 1

Summary

Summary: ADP-RIBOSYLATION FACTOR 1 is involved in regulating intracellular transport by modulating the interaction of coat proteins with organelle membranes in the early secretory pathway. It is a component of COAT PROTEIN COMPLEX I. This enzyme was formerly listed as EC 3.6.1.47.

Top Publications

  1. ncbi The GGAs promote ARF-dependent recruitment of clathrin to the TGN
    R Puertollano
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    Cell 105:93-102. 2001
  2. ncbi Specificities for the small G proteins ARF1 and ARF6 of the guanine nucleotide exchange factors ARNO and EFA6
    E Macia
    , CNRS-UMR 6097, 660 route des Lucioles, 06560 Valbonne Sophia-Antipolis, France
    J Biol Chem 276:24925-30. 2001
  3. ncbi Continual production of phosphatidic acid by phospholipase D is essential for antigen-stimulated membrane ruffling in cultured mast cells
    Niamh O'Luanaigh
    Department of Physiology, University College London, London WC1E 6JJ, United Kingdom
    Mol Biol Cell 13:3730-46. 2002
  4. ncbi Phosphatidylinositol 4-phosphate 5-kinase alpha is a downstream effector of the small G protein ARF6 in membrane ruffle formation
    A Honda
    Department of Biological Information, Tokyo Institute of Technology, Yokohama, Japan
    Cell 99:521-32. 1999
  5. ncbi Activation of ADP-ribosylation factor 1 GTPase-activating protein by phosphatidylcholine-derived diacylglycerols
    B Antonny
    CNRS, Institut de Pharmacologie Moleculaire et Cellulaire, 660 route des Lucioles, 06560 Valbonne, France
    J Biol Chem 272:30848-51. 1997
  6. ncbi Cytohesin-1, a cytosolic guanine nucleotide-exchange protein for ADP-ribosylation factor
    E Meacci
    Pulmonary Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1590, USA
    Proc Natl Acad Sci U S A 94:1745-8. 1997
  7. ncbi ADP-ribosylation factor is functionally and physically associated with the Golgi complex
    T Stearns
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139
    Proc Natl Acad Sci U S A 87:1238-42. 1990
  8. ncbi Sequential coupling between COPII and COPI vesicle coats in endoplasmic reticulum to Golgi transport
    M Aridor
    Scripps Research Institute, Department of Cell Biology, La Jolla, California 92037, USA
    J Cell Biol 131:875-93. 1995
  9. ncbi Membrane curvature induced by Arf1-GTP is essential for vesicle formation
    Rainer Beck
    Biochemistry Center, Heidelberg University, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    Proc Natl Acad Sci U S A 105:11731-6. 2008
  10. ncbi ARNO is a guanine nucleotide exchange factor for ADP-ribosylation factor 6
    S Frank
    Combined Program in Pediatric Gastroenterology and Nutrition, Massachusetts General Hospital East, Charlestown, Masschusetts 02129, USA
    J Biol Chem 273:23-7. 1998

Research Grants

  1. BIOSYNTHESIS AND PROCESSING OF PEPTIDE HORMONE
    Dennis Shields; Fiscal Year: 2003
  2. Structural Study of ARF-GTPase Regulators and Effectors
    Jonathan Goldberg; Fiscal Year: 2007
  3. STRUCTURAL STUDY OF ARF GTPASE REGULATORS AND EFFECTORS
    Jonathan Goldberg; Fiscal Year: 2003
  4. Molecular Mechanisms of HIV Disease Pathogenesis
    Kathleen Collins; Fiscal Year: 2007
  5. Molecular Mechanisms of HIV's Immune Evasion
    Kathleen Collins; Fiscal Year: 2007
  6. Molecular Mechanisms of HIV's Immune Evasion
    Kathleen L Collins; Fiscal Year: 2010
  7. Cargo sorting during endocytic recycling
    Victor Hsu; Fiscal Year: 2007
  8. BIOGENESIS OF TRANSPORT VESICLES COATED BY COPI
    Victor W Hsu; Fiscal Year: 2010
  9. BIOGENESIS OF TRANSPORT VESICLES COATED BY COPI
    Victor Hsu; Fiscal Year: 2007
  10. BIOGENESIS OF TRANSPORT VESICLES COATED BY COPI
    Victor Hsu; Fiscal Year: 2006

Detail Information

Publications171 found, 100 shown here

  1. ncbi The GGAs promote ARF-dependent recruitment of clathrin to the TGN
    R Puertollano
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
    Cell 105:93-102. 2001
    ..These observations suggest that the GGAs could function to link clathrin to membrane-bound ARF.GTP...
  2. ncbi Specificities for the small G proteins ARF1 and ARF6 of the guanine nucleotide exchange factors ARNO and EFA6
    E Macia
    , CNRS-UMR 6097, 660 route des Lucioles, 06560 Valbonne Sophia-Antipolis, France
    J Biol Chem 276:24925-30. 2001
    ..Thus selectivity for ARF1 or ARF6 must depend on subtle folding differences between the ARFs switch regions that interact with the Sec7 domains...
  3. ncbi Continual production of phosphatidic acid by phospholipase D is essential for antigen-stimulated membrane ruffling in cultured mast cells
    Niamh O'Luanaigh
    Department of Physiology, University College London, London WC1E 6JJ, United Kingdom
    Mol Biol Cell 13:3730-46. 2002
    ..We conclude that both activation of ARF6 by antigen and a continual PLD2 activity are essential for local phosphatidylinositol(4,5)bisphosphate generation that regulates dynamic actin cytoskeletal rearrangements...
  4. ncbi Phosphatidylinositol 4-phosphate 5-kinase alpha is a downstream effector of the small G protein ARF6 in membrane ruffle formation
    A Honda
    Department of Biological Information, Tokyo Institute of Technology, Yokohama, Japan
    Cell 99:521-32. 1999
    ....
  5. ncbi Activation of ADP-ribosylation factor 1 GTPase-activating protein by phosphatidylcholine-derived diacylglycerols
    B Antonny
    CNRS, Institut de Pharmacologie Moleculaire et Cellulaire, 660 route des Lucioles, 06560 Valbonne, France
    J Biol Chem 272:30848-51. 1997
    ..Thus, the phospholipase D pathway may provide a feedback mechanism that promotes GTP hydrolysis on ARF1 and the consequent uncoating of vesicles...
  6. ncbi Cytohesin-1, a cytosolic guanine nucleotide-exchange protein for ADP-ribosylation factor
    E Meacci
    Pulmonary Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892 1590, USA
    Proc Natl Acad Sci U S A 94:1745-8. 1997
    ..In this regard, it differs from a recently reported BFA-sensitive ARF-GEP that contains a Sec7 domain...
  7. ncbi ADP-ribosylation factor is functionally and physically associated with the Golgi complex
    T Stearns
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139
    Proc Natl Acad Sci U S A 87:1238-42. 1990
    ..Together, these results indicate that ARF functions in intracellular protein transport to or within the Golgi apparatus, a role not predicted by the previous in vitro biochemical studies...
  8. ncbi Sequential coupling between COPII and COPI vesicle coats in endoplasmic reticulum to Golgi transport
    M Aridor
    Scripps Research Institute, Department of Cell Biology, La Jolla, California 92037, USA
    J Cell Biol 131:875-93. 1995
    ..We propose that sequential coupling between COPII and COPI coats is essential to coordinate and direct bi-directional vesicular traffic between the ER and pre-Golgi intermediates involved in transport of protein to the Golgi complex...
  9. ncbi Membrane curvature induced by Arf1-GTP is essential for vesicle formation
    Rainer Beck
    Biochemistry Center, Heidelberg University, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    Proc Natl Acad Sci U S A 105:11731-6. 2008
    ..Strikingly, this mutant was not able to deform membranes, suggesting that GTP-induced dimerization of Arf1 is a critical step inducing membrane curvature during the formation of coated vesicles...
  10. ncbi ARNO is a guanine nucleotide exchange factor for ADP-ribosylation factor 6
    S Frank
    Combined Program in Pediatric Gastroenterology and Nutrition, Massachusetts General Hospital East, Charlestown, Masschusetts 02129, USA
    J Biol Chem 273:23-7. 1998
    ..These findings are consistent with the previous observation that cytohesin-1 regulates the adhesiveness of alphaLbeta2 integrins at the plasma membrane of lymphocytes...
  11. ncbi Arfs, phosphoinositides and membrane traffic
    J G Donaldson
    Laboratory of Cell Biology, National Heart, Lung and Blood Institute, National Institutes of Health, Building 50, Rm 2503, Bethesda, MD 20892, USA
    Biochem Soc Trans 33:1276-8. 2005
    ..Arf1 can also stimulate the activity of phosphatidylinositol kinases and recruit coat proteins and actin cytoskeletal elements to the Golgi complex...
  12. ncbi Turning on ARF: the Sec7 family of guanine-nucleotide-exchange factors
    C L Jackson
    Service de Biochimie et Génétique Moléculaire, CEA Saclay, Gif sur Yvette, France
    Trends Cell Biol 10:60-7. 2000
    ..A separate subclass of Sec7-domain proteins is involved in signal transduction and possess a domain that mediates membrane binding in response to extracellular signals...
  13. ncbi GGAs: a family of ADP ribosylation factor-binding proteins related to adaptors and associated with the Golgi complex
    E C Dell'Angelica
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 149:81-94. 2000
    ..Treatment with brefeldin A or overexpression of dominant-negative ADP ribosylation factor 1 (ARF1) caused dissociation of GGAs from membranes...
  14. ncbi Distinct roles for the yeast phosphatidylinositol 4-kinases, Stt4p and Pik1p, in secretion, cell growth, and organelle membrane dynamics
    A Audhya
    Division of Cellular and Molecular Medicine, The Howard Hughes Medical Institute, University of California, San Diego 92093 0668, USA
    Mol Biol Cell 11:2673-89. 2000
    ....
  15. ncbi Poliovirus proteins induce membrane association of GTPase ADP-ribosylation factor
    George A Belov
    Laboratory of Infectious Diseases, NIAID, NIH, Building 50, Room 6120, Bethesda, MD 20892-8011, USA
    J Virol 79:7207-16. 2005
    ..Taken together, the data suggest an involvement of ARF in poliovirus RNA replication...
  16. ncbi Reconstitution of retrograde transport from the Golgi to the ER in vitro
    A Spang
    Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California, Berkeley, California 94720, USA
    J Cell Biol 143:589-99. 1998
    ..Putative retrograde carriers (COPI vesicles) generated from Golgi-enriched membranes contain v-SNAREs as well as Emp47p as cargo...
  17. ncbi COPI vesicles accumulating in the presence of a GTP restricted arf1 mutant are depleted of anterograde and retrograde cargo
    R Pepperkok
    Cell Biophysics and Cell Biology Program, EMBL Heidelberg, Meyerhofstr 1, Germany
    J Cell Sci 113:135-44. 2000
    ....
  18. ncbi An arf1Delta synthetic lethal screen identifies a new clathrin heavy chain conditional allele that perturbs vacuolar protein transport in Saccharomyces cerevisiae
    C Y Chen
    Department of Molecular Biology, Vanderbilt University, Nashville, Tennessee 37235, USA
    Genetics 150:577-89. 1998
    ....
  19. ncbi Structural basis for activation of ARF GTPase: mechanisms of guanine nucleotide exchange and GTP-myristoyl switching
    J Goldberg
    Cellular Biochemistry and Biophysics Program Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Cell 95:237-48. 1998
    ..The transition controls the exposure of the myristoylated N terminus, explaining how ARF GTPases couple the GDP-GTP conformational switch to membrane binding...
  20. ncbi Molecular mechanism of membrane recruitment of GGA by ARF in lysosomal protein transport
    Tomoo Shiba
    Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization KEK, Tsukuba, Ibaraki 305 0801, Japan
    Nat Struct Biol 10:386-93. 2003
    ..These data reveal a molecular mechanism underlying membrane recruitment of adaptor proteins by ARF-GTP...
  21. ncbi ADP-ribosylation factor 6 as a target of guanine nucleotide exchange factor GRP1
    S E Langille
    Program in Molecular Medicine, Worcester, Massachusetts 01605, USA
    J Biol Chem 274:27099-104. 1999
    ..Further, co-localization of GRP1 with ARF6, combined with its demonstrated ability to activate ARF6, suggests a physiological role for GRP1 in regulating ARF6 functions...
  22. ncbi ARF1 is directly involved in dynamin-independent endocytosis
    Sudha Kumari
    National Centre for Biological Science TIFR, Bellary Road, Bangalore 560 065, India
    Nat Cell Biol 10:30-41. 2008
    ..These findings provide a molecular basis for a crosstalk of endocytosis with secretion by the sharing of a key regulator of secretory traffic, ARF1...
  23. ncbi Retrograde transport from the yeast Golgi is mediated by two ARF GAP proteins with overlapping function
    P P Poon
    Department of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 4H7
    EMBO J 18:555-64. 1999
    ..An in vitro assay indicates that efficient retrieval from the Golgi to the ER requires these two proteins. These findings suggest that Glo3 and Gcs1 ARF GAPs mediate retrograde vesicular transport from the Golgi to the ER...
  24. ncbi Evidence for a COP-I-independent transport route from the Golgi complex to the endoplasmic reticulum
    A Girod
    Cell Biology and Biophysics Programme, EMBL Heidelberg, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Nat Cell Biol 1:423-30. 1999
    ..The COP-I-independent pathway is specifically regulated by Rab6 and is used by Golgi glycosylation enzymes and Shiga toxin/Shiga-like toxin-1...
  25. ncbi ARF1.GTP, tyrosine-based signals, and phosphatidylinositol 4,5-bisphosphate constitute a minimal machinery to recruit the AP-1 clathrin adaptor to membranes
    Pascal Crottet
    Biozentrum, University of Basel, CH 4056 Basel, Switzerland
    Mol Biol Cell 13:3672-82. 2002
    ..The results thus indicate that cargo proteins are involved in coat recruitment and that the local lipid composition contributes to specifying the site of vesicle formation...
  26. ncbi Dissection of COPI and Arf1 dynamics in vivo and role in Golgi membrane transport
    John F Presley
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nature 417:187-93. 2002
    ..This role for Arf1/coatomer might provide a model for investigating the behaviour of other coat protein systems within cells...
  27. ncbi ARF-GAP-mediated interaction between the ER-Golgi v-SNAREs and the COPI coat
    Ulrike Rein
    Friedrich Miescher Laboratory, Max Planck Society, D 72076 Tubingen, Germany
    J Cell Biol 157:395-404. 2002
    ..The mechanisms by which v-SNAREs interact with COPI and COPII coat proteins seem to be different and may play a key role in determining specificity in vesicle budding...
  28. ncbi Arf and its many interactors
    Zhongzhen Nie
    Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, Building 37, Room 4118, Bethesda, MD 20892, USA
    Curr Opin Cell Biol 15:396-404. 2003
    ..Recent work examining the functional relationships among the diverse Arf interactors has led to reconsideration of the prevailing paradigms for Arf action...
  29. ncbi Overexpression of an ADP-ribosylation factor-guanine nucleotide exchange factor, BIG2, uncouples brefeldin A-induced adaptor protein-1 coat dissociation and membrane tubulation
    Chisa Shinotsuka
    Institute of Biological Sciences and Gene Research Center, University of Tsukuba, 1 1 1 Tennohdai, Tsukuba Science City, Ibaraki 305 8572, Japan
    J Biol Chem 277:9468-73. 2002
    ..These observations indicate that BFA-induced AP-1 dissociation from TGN membranes and tubulation of TGN membranes are not coupled events and suggest that a BFA target other than ARF-GEFs exists in the cell...
  30. ncbi DEF-1/ASAP1 is a GTPase-activating protein (GAP) for ARF1 that enhances cell motility through a GAP-dependent mechanism
    Craig Furman
    Department of Cancer Biology, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 277:7962-9. 2002
    ..In contrast, the inhibition of cell spreading by DEF-1 was not dependent on GAP activity, indicating that spreading and motility are altered by DEF-1 through different pathways...
  31. ncbi Sorting of Golgi resident proteins into different subpopulations of COPI vesicles: a role for ArfGAP1
    J Lanoix
    Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, D 69017 Heidelberg, Germany
    J Cell Biol 155:1199-212. 2001
    ..We propose a two-stage reaction to explain how GTP hydrolysis constitutes a prerequisite for sorting of resident proteins, yet becomes inhibited in their presence...
  32. ncbi Recruitment to Golgi membranes of ADP-ribosylation factor 1 is mediated by the cytoplasmic domain of p23
    D U Gommel
    Biochemie Zentrum Heidelberg, Ruprecht Karls Universitat, Im Neuenheimer Feld 328, D 69120 Heidelberg, Germany
    EMBO J 20:6751-60. 2001
    Binding to Golgi membranes of ADP ribosylation factor 1 (ARF1) is the first event in the initiation of COPI coat assembly. Based on binding studies, a proteinaceous receptor has been proposed to be critical for this process...
  33. ncbi KDEL-cargo regulates interactions between proteins involved in COPI vesicle traffic: measurements in living cells using FRET
    I Majoul
    Department of Neurobiology, Max Planck Institute of Biophysical Chemistry, Gottingen, Germany
    Dev Cell 1:139-53. 2001
    ..Both p24a and p23 interact with ARF1, but only p24 interacts with ARFGAP. These findings suggest a model for how cargo-induced oligomerization of ERD2 regulates its sorting into COPI-coated buds...
  34. ncbi Regulation of GTP hydrolysis on ADP-ribosylation factor-1 at the Golgi membrane
    E Szafer
    Department of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel
    J Biol Chem 276:47834-9. 2001
    ....
  35. ncbi ArfGAP1 dynamics and its role in COPI coat assembly on Golgi membranes of living cells
    Wei Liu
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 168:1053-63. 2005
    ..These data suggest that ArfGAP1, coatomer and Arf1 play interdependent roles in the assembly-disassembly cycle of the COPI coat in vivo...
  36. ncbi Multiple activities for Arf1 at the Golgi complex
    Julie G Donaldson
    Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Building 50, Room 2503, Bethesda, MD 20892, USA
    Biochim Biophys Acta 1744:364-73. 2005
    ..This spatial distribution of Arf regulators may facilitate the recruitment of the coat proteins and other Arf effectors to different regions of the Golgi complex...
  37. ncbi Targeting of Arf-1 to the early Golgi by membrin, an ER-Golgi SNARE
    Akira Honda
    Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 168:1039-51. 2005
    ..These studies suggest that membrin recruits Arf-1 to the early Golgi and reveal distinct kinetic cycles for Arf-1 at early and late Golgi determined by different sets of Arf regulators and effectors...
  38. ncbi ARF1 regulatory factors and COPI vesicle formation
    Anne Spang
    Friedrich Miescher Laboratorium, Max Planck Gesellschaft, Spemannstrasse 39 D 72076 Tübingen, Germany
    Curr Opin Cell Biol 14:423-7. 2002
    ....
  39. ncbi The structural GDP/GTP cycle of human Arf6
    S Pasqualato
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS, 1, Avenue de la Terrasse, 91198 Gif sur Yvette cedex and Institut de Pharmacologie Moléculaire et Cellulaire, CNRS, 660 route des Lucioles, 06560 Valbonne, France
    EMBO Rep 2:234-8. 2001
    ..The structure also allows further insight into the lack of spontaneous GTPase activity of Arf proteins...
  40. ncbi Membrane curvature and the control of GTP hydrolysis in Arf1 during COPI vesicle formation
    B Antonny
    Institut de Pharmacologie Moleculaire et Cellulaire, CNRS et Université de Nice, 660 route des Lucioles, 06560 Valbonne Sophia Antipolis, France
    Biochem Soc Trans 33:619-22. 2005
    ..As a result, the coat should be stable as long as the bud remains attached and should disassemble as soon as membrane fission occurs...
  41. ncbi Targeting of Golgi-specific pleckstrin homology domains involves both PtdIns 4-kinase-dependent and -independent components
    Timothy P Levine
    Division of Cell Biology, Institute of Ophthalmology, University College London, Bath Street, London EC1V 9EL, UK
    Curr Biol 12:695-704. 2002
    ....
  42. ncbi Coatomer, Arf1p, and nucleotide are required to bud coat protein complex I-coated vesicles from large synthetic liposomes
    A Spang
    Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 95:11199-204. 1998
    ..We conclude that Arf1p-GTP and coatomer comprise the minimum apparatus necessary to create a COPI-coated vesicle...
  43. ncbi Human ADP-ribosylation factors. A functionally conserved family of GTP-binding proteins
    R A Kahn
    Laboratory of Biological Chemistry, National Cancer Institute, Bethesda, Maryland 20892
    J Biol Chem 266:2606-14. 1991
    ....
  44. ncbi Crystal structure of ARF1*Sec7 complexed with Brefeldin A and its implications for the guanine nucleotide exchange mechanism
    Elena Mossessova
    Howard Hughes Medical Institute, Structural Biology Program, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
    Mol Cell 12:1403-11. 2003
    ..Thus, the Sec7 domain may act as a dual catalyst, facilitating both nucleotide release and conformational switching on ARF proteins...
  45. ncbi Trypanosoma brucei ARF1 plays a central role in endocytosis and golgi-lysosome trafficking
    Helen P Price
    Immunology and Infection Unit, Department of Biology, University of York, Heslington, York YO10 5YW, United Kingdom
    Mol Biol Cell 18:864-73. 2007
    ..We conclude that the essential Golgi-localizing T. brucei ARF1 has a primary role in the maintenance of both post-Golgi transport and endocytosis and that it is significantly divergent from other characterized ARFs...
  46. ncbi Active Arf6 recruits ARNO/cytohesin GEFs to the PM by binding their PH domains
    Lee Ann Cohen
    Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, and Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Biol Cell 18:2244-53. 2007
    ..This interaction was direct and required both inositol phospholipids and GTP. We propose a model of sequential Arf activation at the PM whereby Arf6-GTP recruits ARNO family GEFs for further activation of other Arf isoforms...
  47. ncbi Dynamics of GBF1, a Brefeldin A-sensitive Arf1 exchange factor at the Golgi
    Ting Kuang Niu
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Biol Cell 16:1213-22. 2005
    ..These results suggest that an Arf1-GBF1-BFA complex is formed and has a longer residence time on Golgi membranes than GBF1 or Arf1 alone...
  48. ncbi Comparative activity of ADP-ribosylation factor family members in the early steps of coated vesicle formation on rat liver Golgi membranes
    J O Liang
    Department of Medicine, Washington University, St Louis, Missouri 63110, USA
    J Biol Chem 272:4141-8. 1997
    ..These data indicate that the specificity for ARF action on the Golgi membranes is primarily determined by the Golgi guanine nucleotide exchange factor, which has a strong preference for myristoylated mammalian ARF1...
  49. ncbi The GTPase Arf1p and the ER to Golgi cargo receptor Erv14p cooperate to recruit the golgin Rud3p to the cis-Golgi
    Alison K Gillingham
    Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 2QH, England, UK
    J Cell Biol 167:281-92. 2004
    ..Tassin, C. Fedriani, and M. Bornens. 2004. Cell. 118:323-335). In contrast, we find that this region binds to the Golgi in a GRAB domain-dependent manner, suggesting that GMAP-210 may not link the Golgi to gamma-tubulin and centrosomes...
  50. ncbi Integrating three views of Arf1 activation dynamics
    Charles H Robert
    , CNRS UMR 8619, , , France
    J Mol Biol 337:969-83. 2004
    ..Large-scale collective movements in the Arf1-Sec7d complex appear to participate in the insertion of the Sec7d Glu finger into the GDP binding site to promote actual nucleotide release...
  51. ncbi ARF1 regulates Nef-induced CD4 degradation
    Julien Faure
    Department of Biochemistry, University of Geneva, 1211 Geneva, Switzerland
    Curr Biol 14:1056-64. 2004
    ..The HIV Nef protein downregulates CD4 through sequential connection with clathrin-coated pits and the COP1 coatomer, resulting in accelerated endocytosis and lysosomal targeting...
  52. ncbi p619, a giant protein related to the chromosome condensation regulator RCC1, stimulates guanine nucleotide exchange on ARF1 and Rab proteins
    J L Rosa
    Department of Molecular Oncology, Bristol Myers Squibb, Pharmaceutical Research Institute, Princeton, NJ 08543, USA
    EMBO J 15:4262-73. 1996
    ..These observations suggest that p619 is a Brefeldin A-sensitive Golgi protein that functions as a guanine nucleotide exchange factor for ARF1 and, possibly, for members of the Rab family of proteins...
  53. ncbi Saccharomyces cerevisiae Gcs1 is an ADP-ribosylation factor GTPase-activating protein
    P P Poon
    Department of Microbiology and Immunology, Dalhousie University, Halifax, NS Canada
    Proc Natl Acad Sci U S A 93:10074-7. 1996
    ..Thus by both in vitro and in vivo criteria, Gcs1 is a yeast ARF GAP...
  54. ncbi Decoding of sorting signals by coatomer through a GTPase switch in the COPI coat complex
    J Goldberg
    Cellular Biochemistry and Biophysics Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Cell 100:671-9. 2000
    ....
  55. ncbi Asymmetric tethering of flat and curved lipid membranes by a golgin
    Guillaume Drin
    Institut de Pharmacologie Moleculaire et Cellulaire, Université de Nice Sophia Antipolis and CNRS, 660 route des Lucioles, 06560 Valbonne, France
    Science 320:670-3. 2008
    ..Because membrane curvature constantly changes during vesicular trafficking, this mode of tethering suggests a way to maintain the Golgi architecture without compromising membrane flow...
  56. ncbi Role of ADP ribosylation factor 1 in the assembly and secretion of ApoB-100-containing lipoproteins
    Lennart Asp
    Department of Medical Biochemistry, Wallenberg Laboratory for Cardiovascular Research, Goteborg University, Goteborg, Sweden
    Arterioscler Thromb Vasc Biol 25:566-70. 2005
    We investigated the role of ADP ribosylation factor 1 (ARF1) in the assembly of very-low-density lipoproteins (VLDLs).
  57. ncbi A regulatory role for ARF6 in receptor-mediated endocytosis
    C D'SOUZA-SCHOREY
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, MO 63110
    Science 267:1175-8. 1995
    ..In contrast, overexpression of ARF1 had little or no effect on these parameters of endocytosis...
  58. ncbi ADP ribosylation factor is an essential protein in Saccharomyces cerevisiae and is encoded by two genes
    T Stearns
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139
    Mol Cell Biol 10:6690-9. 1990
    ..Among revertants of the fluoride sensitivity of an arf1 null mutation were ARF1-ARF2 fusion genes created by a gene conversion event in which the deleted ARF1 sequences were repaired by recombination with ARF2...
  59. ncbi A family of Arf effectors defined as suppressors of the loss of Arf function in the yeast Saccharomyces cerevisiae
    C j Zhang
    Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322 3050, USA
    J Biol Chem 273:19792-6. 1998
    ....
  60. ncbi Membrane recruitment of effector proteins by Arf and Rab GTPases
    Masato Kawasaki
    Structural Biology Research Center, Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK, Tsukuba, Ibaraki 305-0801, Japan
    Curr Opin Struct Biol 15:681-9. 2005
    ....
  61. ncbi Structure of the Sec7 domain of the Arf exchange factor ARNO
    J Cherfils
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS UPR 9063, Gif sur Yvette, France
    Nature 392:101-5. 1998
    ..Here we report the crystal structure of the Sec7 domain of human ARNO at 2 A resolution and the identification of the site of interaction of ARNO with Arf...
  62. ncbi ADP-ribosylation factor 1 controls the activation of the phosphatidylinositol 3-kinase pathway to regulate epidermal growth factor-dependent growth and migration of breast cancer cells
    Pierre Luc Boulay
    Department of Pharmacology, Faculty of Medicine, University of Montreal, Montreal, Quebec H3C 3J7, Canada
    J Biol Chem 283:36425-34. 2008
    ..These results uncover a novel molecular mechanism by which ARF1 regulates breast cancer cell growth and invasion during cancer progression...
  63. ncbi Reversible dissociation of coatomer: functional characterization of a beta/delta-coat protein subcomplex
    J Pavel
    Biochemie Zentrum Heidelberg Ruprecht Karls Universität, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    Proc Natl Acad Sci U S A 95:2140-5. 1998
    ..Herein we describe isolation of a subcomplex of coatomer consisting of beta- and delta-COPs that is able to bind to Golgi membranes in an ARF1- and GTP-dependent manner...
  64. ncbi The ARF1 GTPase-activating protein: zinc finger motif and Golgi complex localization
    E Cukierman
    Department of Biology, Technion Israel Institute of Technology, Haifa, Israel
    Science 270:1999-2002. 1995
    ..Thus, the GAP is likely to be recruited to the Golgi by an ARF1-dependent mechanism...
  65. ncbi Functional reconstitution of COPI coat assembly and disassembly using chemically defined components
    Constanze Reinhard
    Biochemie Zentrum Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany
    Proc Natl Acad Sci U S A 100:8253-7. 2003
    ..Bourgoin, S., Randazzo, P. A., et al. (2002) J. Cell Biol. 159, 69-78]. Thus, a complete round of COPI coat assembly and disassembly has been reconstituted with purified components defining the core machinery of COPI vesicle biogenesis...
  66. ncbi Real-time assays for the assembly-disassembly cycle of COP coats on liposomes of defined size
    Joëlle Bigay
    Institut de Pharmacologie, Moleculaire et Cellulaire, CNRS, Valbonne, France
    Methods Enzymol 404:95-107. 2005
    ..A flotation assay to assess the effect of membrane curvature on protein adsorption to liposomes is also presented...
  67. ncbi Mutational analysis of Saccharomyces cerevisiae ARF1
    R A Kahn
    Laboratory of Biological Chemistry, NCI, National Institutes of Health, Bethesda, Maryland 20892
    J Biol Chem 270:143-50. 1995
    ..Two high-copy suppressors of this conditional phenotype were cloned and sequenced. One of these suppressors, SFS4, was found to be identical to PBS2/HOG4, recently shown to encode a microtubule-associated protein kinase kinase in yeast...
  68. ncbi Specific regulation of the adaptor protein complex AP-3 by the Arf GAP AGAP1
    Zhongzhen Nie
    Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, Building 37, Room 4118, Bethesda, MD 20892, USA
    Dev Cell 5:513-21. 2003
    ..Cells overexpressing AGAP1 also exhibit increased LAMP1 trafficking via the plasma membrane. Taken together, these results support the hypothesis that AGAP1 directly and specifically regulates AP-3-dependent trafficking...
  69. ncbi Targeting of the Arf-like GTPase Arl3p to the Golgi requires N-terminal acetylation and the membrane protein Sys1p
    Rudy Behnia
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Nat Cell Biol 6:405-13. 2004
    ..This suggests that the targeting of ARFRP1/Arl3p to the Golgi is mediated by a direct interaction between its acetylated N terminus and Sys1p/hSys1...
  70. ncbi ADP-ribosylation factor 1 of Arabidopsis plays a critical role in intracellular trafficking and maintenance of endoplasmic reticulum morphology in Arabidopsis
    Mi Hee Lee
    Center for Plant Intracellular Trafficking, Pohang University of Science and Technology, Pohang, 790-784, Korea
    Plant Physiol 129:1507-20. 2002
    ..Together, these results strongly suggest that Arf1 plays a role in the intracellular trafficking of cargo proteins in Arabidopsis, and that Arf1 functions through a brefeldin A-sensitive factor...
  71. ncbi Growth factors mobilize multiple pools of KCa channels in developing parasympathetic neurons: role of ADP-ribosylation factors and related proteins
    Kwon-Seok Chae
    Department of Biology and Biochemistry, University of Houston, Houston, TX 77204-5513, USA
    J Neurophysiol 94:1597-605. 2005
    ....
  72. ncbi Differences between AGAP1, ASAP1 and Arf GAP1 in substrate recognition: interaction with the N-terminus of Arf1
    Hye-Young Yoon
    Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, Building 37, Room 4118, National Institutes of Health, Bethesda, MD 20892, USA
    Cell Signal 16:1033-44. 2004
    ..Taken together, our results indicate that (i) Arf GAPs interact with amino acids 2-17 of Arf1 and (ii) each subgroup of Arf GAPs has a unique interface with Arf1...
  73. ncbi A novel Golgi membrane protein is a partner of the ARF exchange factors Gea1p and Gea2p
    Sophie Chantalat
    SBGM, CEA Saclay, 91191 Gif sur Yvette Cedex, France
    Mol Biol Cell 14:2357-71. 2003
    ..Hence, we propose that Gmh1p acts as a positive Golgi-membrane partner for Gea function. These results are of general interest given the evolutionary conservation of both ARF-GEFs and the Gmh proteins...
  74. ncbi The GTPase ARF1p controls the sequence-specific vacuolar sorting route to the lytic vacuole
    Peter Pimpl
    Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, United Kingdom
    Plant Cell 15:1242-56. 2003
    ..This finding suggests that ARF1p influences the BP80-mediated transport route to the vacuole in addition to transport steps of the default secretory pathway to the cell surface...
  75. ncbi Arf1 GTPase plays roles in the protein traffic between the endoplasmic reticulum and the Golgi apparatus in tobacco and Arabidopsis cultured cells
    Masaki Takeuchi
    Molecular Membrane Biology Laboratory, Plant Science Center, RIKEN, Wako, Saitama 351-0198, Japan
    Plant J 31:499-515. 2002
    ..These results indicate that AtArf1 play roles in the vesicular transport between the ER and the Golgi and in the maintenance of the normal Golgi organization in plant cells...
  76. ncbi Recycling of Raft-associated prohormone sorting receptor carboxypeptidase E requires interaction with ARF6
    Irina Arnaoutova
    Section of Cellular Neurobiology, Laboratory of Developmental Neurobiology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    Mol Biol Cell 14:4448-57. 2003
    ..Thus, CPE recycles back to the TGN by a novel mechanism requiring ARF6 interaction and activity...
  77. ncbi Spatiotemporal dynamics of the COPI vesicle machinery
    Markus Elsner
    Cell Biology and Cell Biophysics Programme, EMBL, Meyerhofstrasse 1, D-69117 Heidelberg, Germany
    EMBO Rep 4:1000-4. 2003
    Assembly of the coat protein I (COPI) vesicle coat is controlled by the small GTPase ADP ribosylation factor 1 (ARF1) and its GTPase-activating protein, ARFGAP1...
  78. ncbi A kinetic proof-reading mechanism for protein sorting
    Matthias Weiss
    Cell Biology and Cell Biophysics Programme, EMBL, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    Traffic 4:65-73. 2003
    ..The sorting scheme predicts a dependency on the amount of ARFGAP1 and explains the recent experimental findings that ARF-1 and COPI detach with different time constants from the Golgi membrane in vivo...
  79. ncbi ARFGAP1 plays a central role in coupling COPI cargo sorting with vesicle formation
    Stella Y Lee
    Division of Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital, and Department of Medicine, Harvard Medical School, Boston, MA 02115
    J Cell Biol 168:281-90. 2005
    ..key components of coat protein I (COPI) transport participate in cargo sorting, we find that, instead of ADP ribosylation factor 1 (ARF1), its GTPase-activating protein (GAP) plays a direct role in promoting the binding of cargo ..
  80. ncbi Differential expression and targeting of endogenous Arf1 and Arf6 small GTPases in kidney epithelial cells in situ
    Jaafar El-Annan
    Program in Membrane Biology and Renal Unit, Massachusetts General Hospital, Boston, MA 02129 2020, USA
    Am J Physiol Cell Physiol 286:C768-78. 2004
    ..The results provide a framework on which to base and interpret future studies on the role of Arf GTPases in the multitude of cellular trafficking events that occur in renal tubular epithelial cells...
  81. ncbi [Retrograde transport]
    Tomohiko Aoe
    Tanpakushitsu Kakusan Koso 49:914-5. 2004
  82. ncbi ADP-ribosylation factor-dependent phospholipase D activation by the M3 muscarinic receptor
    Rory Mitchell
    Medical Research Council Membrane and Adapter Proteins Co operative Group, Membrane Biology Interdisciplinary Research Group, School of Biomedical and Clinical Laboratory Sciences, University of Edinburgh, EH8 9XD, United Kingdom
    J Biol Chem 278:33818-30. 2003
    ..Examples of other G protein-coupled receptors assessed in comparison display alternative pathways of protein kinase C- or ARF6-dependent activation of PLD2...
  83. ncbi Analysis of oxysterol binding protein homologue Kes1p function in regulation of Sec14p-dependent protein transport from the yeast Golgi complex
    Xinmin Li
    Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
    J Cell Biol 157:63-77. 2002
    ....
  84. ncbi In tobacco leaf epidermal cells, the integrity of protein export from the endoplasmic reticulum and of ER export sites depends on active COPI machinery
    Giovanni Stefano
    Department of Biology, 112 Science Place, University of Saskatchewan, Saskatoon, SK S7N 5E2, Canada
    Plant J 46:95-110. 2006
    ....
  85. ncbi A viral protein that blocks Arf1-mediated COP-I assembly by inhibiting the guanine nucleotide exchange factor GBF1
    Els Wessels
    Department of Medical Microbiology, Radboud University Nijmegen Medical Centre, Nijmegen Centre for Molecular Life Sciences, PO Box 9101, 6500 HB Nijmegen, The Netherlands
    Dev Cell 11:191-201. 2006
    ..By specifically interfering with GBF1-mediated Arf1 activation, 3A may prove a valuable tool in dissecting the early steps of the secretory pathway...
  86. ncbi The KDEL receptor, ERD2, regulates intracellular traffic by recruiting a GTPase-activating protein for ARF1
    T Aoe
    Division of Rheumatology, Immunology and Allergy, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    EMBO J 16:7305-16. 1997
    ..Because ERD2 overexpression enhances the recruitment of GAP to membranes and results in a phenotype that reflects ARF1 inactivation, our findings suggest that ERD2 regulates ARF1 GAP, and thus regulates ARF1-mediated transport...
  87. ncbi Knockout of Arfrp1 leads to disruption of ARF-like1 (ARL1) targeting to the trans-Golgi in mouse embryos and HeLa cells
    Claudia Zahn
    Department of Pharmacology, German Institute of Human Nutrition Potsdam Rehbruecke, Nuthetal, Germany
    Mol Membr Biol 23:475-85. 2006
    ..These data suggest that lethality of Arfrp1 knockout embryos is due to a specific disruption of protein targeting, e.g., of ARL1 and Golgin-245, to the Golgi...
  88. ncbi Arfaptin 1, a putative cytosolic target protein of ADP-ribosylation factor, is recruited to Golgi membranes
    H Kanoh
    Howard Hughes Medical Institute and the Department of Molecular Physiology and Biophysics and Pharmacology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Biol Chem 272:5421-9. 1997
    ..These results suggest that arfaptins 1 and 2 may be direct target proteins of class 1 ARFs. Arfaptin 1 may be involved in Golgi function along with ARF1...
  89. ncbi The role of ARF1 and rab GTPases in polarization of the Golgi stack
    Serguei I Bannykh
    Department of Pathology, Yale University Medical School, 310 Cedar Street, New Haven, CT 06520, USA
    Traffic 6:803-19. 2005
    ....
  90. ncbi Interaction of SNAREs with ArfGAPs precedes recruitment of Sec18p/NSF
    Christina Schindler
    Biozentrum, University of Basel, CH 4056 Basel, Switzerland
    Mol Biol Cell 18:2852-63. 2007
    ....
  91. ncbi The Arf GEF GBF1 is required for GGA recruitment to Golgi membranes
    Stephane Lefrancois
    Hopital Maisonneuve Rosemont, Universite de Montreal, Montreal, Canada
    Traffic 8:1440-51. 2007
    ..In summary, we show that GBF1 is required for GGA recruitment to Golgi membranes and plays a role in the proper processing and sorting of lysosomal cargo...
  92. ncbi Dissecting the role of the ARF guanine nucleotide exchange factor GBF1 in Golgi biogenesis and protein trafficking
    Tomasz Szul
    Department of Cell Biology, University of Alabama at Birmingham, 1918 University Blvd, Birmingham, AL 35924, USA
    J Cell Sci 120:3929-40. 2007
    ..However, the trafficking of transmembrane proteins through the existing pathway requires GBF1-mediated ARF activation and COPI recruitment...
  93. ncbi COPII vesicles derived from mammalian endoplasmic reticulum microsomes recruit COPI
    T Rowe
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Cell Biol 135:895-911. 1996
    ..These results suggest that coat exchange is an early event preceding the targeting of ER-derived vesicles to pre-Golgi intermediates...
  94. ncbi Bap31 is an itinerant protein that moves between the peripheral endoplasmic reticulum (ER) and a juxtanuclear compartment related to ER-associated Degradation
    Yuichi Wakana
    School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192 0392, Japan
    Mol Biol Cell 19:1825-36. 2008
    ..Overexpression of Sar1p and Arf1 mutants affected Bap31 cycling, suggesting that this cycling pathway is related to the conventional vesicular transport pathways...
  95. ncbi Dominant inhibitory mutants of ARF1 block endoplasmic reticulum to Golgi transport and trigger disassembly of the Golgi apparatus
    C Dascher
    Department of Cell and Molecular Biology, Scripps Research Institute, La Jolla, California 92037
    J Biol Chem 269:1437-48. 1994
    ..These results are discussed in the context of the GDP and GTP-bound forms of ARF in controlling both membrane structure and vesicular traffic through the early secretory pathway...
  96. ncbi Mutational analysis of the Arf1*GTP/Arf GAP interface reveals an Arf1 mutant that selectively affects the Arf GAP ASAP1
    RuiBai Luo
    Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Curr Biol 15:2164-9. 2005
    ..Based on our results, we conclude that the contribution of specific residues within switch 1 of Arf to binding and achieving a transition state toward GTP hydrolysis differs among Arf GAPs...
  97. ncbi ARNO3, a Sec7-domain guanine nucleotide exchange factor for ADP ribosylation factor 1, is involved in the control of Golgi structure and function
    M Franco
    Centre d Immunologie Institut National de la Santé et de la Recherche Médicale Centre National de la Recherche Scientifique de Marseille Luminy, Case 906, 13288 Marseille Cedex 9, France
    Proc Natl Acad Sci U S A 95:9926-31. 1998
    ..This study indicates that Sec7 domain-containing GEFs control intracellular membrane compartment structure and function through the regulation of specific ARF proteins in mammalian cells...
  98. ncbi Reevaluation of the effects of brefeldin A on plant cells using tobacco Bright Yellow 2 cells expressing Golgi-targeted green fluorescent protein and COPI antisera
    Christophe Ritzenthaler
    Institut de Biologie Moleculaire des Plantes, 67084 Strasbourg Cedex, France
    Plant Cell 14:237-61. 2002
    ....
  99. ncbi Maintenance of Golgi structure and function depends on the integrity of ER export
    T H Ward
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    J Cell Biol 155:557-70. 2001
    ..Instead, they suggest that the Golgi complex is a dynamic, steady-state system, whose membranes can be nucleated and are maintained by the activities of the Sar1-COPII and Arf1-coatomer systems...
  100. ncbi Membrane traffic: how do GGAs fit in with the adaptors?
    M W Black
    MRC Laboratory of Molecular Biology, Hills Road, CB2 2QH, Cambridge, UK
    Curr Biol 11:R460-2. 2001
    ..But new results on 'GGA' proteins have raised doubts about this paradigm and suggest both a new sorting mechanism and an unexpected complexity in the roles of clathrin...
  101. ncbi Arf1 dissociates from the clathrin adaptor GGA prior to being inactivated by Arf GTPase-activating proteins
    Kerry M Jacques
    Laboratory of Cellular Oncology, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 277:47235-41. 2002
    ..Instead, termination of Arf1 signals mediated through GGA require that Arf1.GTP dissociates from GGA prior to interaction with GAP and consequent hydrolysis of GTP...

Research Grants75

  1. BIOSYNTHESIS AND PROCESSING OF PEPTIDE HORMONE
    Dennis Shields; Fiscal Year: 2003
    ..Native and epitope tagged versions of ARF-1 will be used in conjunction with cross-linking agents to identify the proposed protein complex. ..
  2. Structural Study of ARF-GTPase Regulators and Effectors
    Jonathan Goldberg; Fiscal Year: 2007
    ..8 Angstroms resolution in-house, which we are working to improve. These studies will provide insight to the processes of GTP-dependent assembly of vesicular coat complexes and coat-controlled GTP hydrolysis triggering disassembly. ..
  3. STRUCTURAL STUDY OF ARF GTPASE REGULATORS AND EFFECTORS
    Jonathan Goldberg; Fiscal Year: 2003
    ..abstract_text> ..
  4. Molecular Mechanisms of HIV Disease Pathogenesis
    Kathleen Collins; Fiscal Year: 2007
    ..This proposal is relevant to the mission of NIH in that it seeks to understand basic mechanisms of how HIV establishes a chronic infection. This work has the potential to have a significant impact on public health. ..
  5. Molecular Mechanisms of HIV's Immune Evasion
    Kathleen Collins; Fiscal Year: 2007
    ..Studies are proposed to better understand the detailed molecular mechanism of MHC-I downmodulation. These studies should aid in the development of inhibitors that disrupt HIV immune evasion. ..
  6. Molecular Mechanisms of HIV's Immune Evasion
    Kathleen L Collins; Fiscal Year: 2010
    ..This grant proposal aims to more fully understand the viral mechanisms for immune escape and viral persistence in order to eradicate infected cells, cure disease and prevent new infection. ..
  7. Cargo sorting during endocytic recycling
    Victor Hsu; Fiscal Year: 2007
    ..abstract_text> ..
  8. BIOGENESIS OF TRANSPORT VESICLES COATED BY COPI
    Victor W Hsu; Fiscal Year: 2010
    ..Thus, as defects in intracellular transport pathways are being appreciated as the cause for a growing number of human diseases, our findings will likely be broadly applicable to understanding and treating these diseases. ..
  9. BIOGENESIS OF TRANSPORT VESICLES COATED BY COPI
    Victor Hsu; Fiscal Year: 2007
    ..Thus, as defects in intracellular transport pathways are being appreciated as the cause for a growing number of human diseases, our findings will likely be broadly applicable to understanding and treating these diseases. ..
  10. BIOGENESIS OF TRANSPORT VESICLES COATED BY COPI
    Victor Hsu; Fiscal Year: 2006
    ....
  11. ARF regulators in endocytic transport
    Victor W Hsu; Fiscal Year: 2011
    ..This understanding will be broadly applicable to understanding human diseases, as many are now appreciated to arise because of defects in protein transport within the cell. ..
  12. BIOGENESIS OF TRANSPORT VESICLES COATED BY COPI
    Victor Hsu; Fiscal Year: 2009
    ..Our results will likely contribute to a basic understanding of transport mechanisms within the cell, and also shed insight into the regulation of iron metabolism. ..
  13. ARF regulators in endocytic transport
    Victor W Hsu; Fiscal Year: 2010
    ..This understanding will be broadly applicable to understanding human diseases, as many are now appreciated to arise because of defects in protein transport within the cell. ..
  14. Albumin-AFP Gene Family Regulation in Fetal and Adult Liver
    Brett T Spear; Fiscal Year: 2010
    ..Since zonal gene regulation is essential for the liver to carry out its normal function, it is important to understand the basis for this control in the normal and diseased liver. ..
  15. Cargo sorting during endocytic recycling
    Victor Hsu; Fiscal Year: 2007
    ..abstract_text> ..
  16. Albumin-AFP Gene Family Regulation in Fetal and Adult Liver
    Brett Spear; Fiscal Year: 2007
    ..Since zonal gene regulation is essential for the liver to carry out its normal function, it is important to understand the basis for this control in the normal and diseased liver. ..
  17. Afri 1: Gene Cloning and its Role in Liver Regulation
    Brett Spear; Fiscal Year: 2006
    ..We can then understand how Afr1 regulates AFP and we are likely to learn more about the transcription/post/transcriptional connections as well as how this may be developmentally regulated to control gene expression. ..
  18. ENHANCERS AND LIVER-SPECIFIC TRANSCRIPTION
    Brett Spear; Fiscal Year: 2001
    ..In addition, the information derived from these studies may improve strategies to express foreign genes within the liver, a goal of many gene therapy trials. ..
  19. AIRWAY SMOOTH MUSCLE IN HEALTH AND DISEASE
    RONALD COBURN; Fiscal Year: 1999
    ..In these investigations we use intact airway smooth muscle, subcellular fractions, and cultured human airway smooth muscle cells. ..
  20. A non-surgical method for producing gene-modified mice
    Brett Spear; Fiscal Year: 2005
    ..We believe that this is a dramatic improvement over current methods, and would become adopted as the method of choice for transgenic researchers if it is truly as successful as our preliminary data suggests. ..
  21. Host proteins in Vaccinia viral membrane biogenesis
    Victor Hsu; Fiscal Year: 2004
    ..abstract_text> ..
  22. AIRWAY SMOOTH MUSCLE IN HEALTH AND DISEASE
    RONALD COBURN; Fiscal Year: 2004
    ..In particular, all of these diseases cause physiological alterations that influence the length at which smooth muscle cells contract and relax. ..
  23. Oxidative stress and IkBalpha modification
    Brett Spear; Fiscal Year: 2002
    ..Overall, these studies may help elucidate additional mechanisms that control NFkB activity in organisms. ..