gtp binding protein beta subunits

Summary

Summary: Heterotrimeric GTP-binding protein subunits that tightly associate with GTP-BINDING PROTEIN GAMMA SUBUNITS. A dimer of beta and gamma subunits is formed when the GTP-BINDING PROTEIN ALPHA SUBUNIT dissociates from the GTP-binding protein heterotrimeric complex. The beta-gamma dimer can play an important role in signal transduction by interacting with a variety of second messengers.

Top Publications

  1. pmc Pheromone signalling in Saccharomyces cerevisiae requires the small GTP-binding protein Cdc42p and its activator CDC24
    Z S Zhao
    Institute of Molecular and Cell Biology, National University of Singapore, Kent Ridge
    Mol Cell Biol 15:5246-57. 1995
  2. ncbi Adenylyl cyclase type II domains involved in Gbetagamma stimulation
    S Weitmann
    , , Thielallee 67 73, 14195 Berlin, Germany
    Biochemistry 40:10853-8. 2001
  3. ncbi Absence of the RGS9.Gbeta5 GTPase-activating complex in photoreceptors of the R9AP knockout mouse
    Gabor Keresztes
    Department of Otolaryngology Eaton Peabody Laboratory, Harvard Medical School, Massachusetts Eye and Ear Infirmary, 243 Charles Street, Boston, MA 02114, USA
    J Biol Chem 279:1581-4. 2004
  4. ncbi Spatial and temporal regulation of RACK1 function and N-methyl-D-aspartate receptor activity through WD40 motif-mediated dimerization
    Claire Thornton
    Ernest Gallo Research Center, Emeryville, California 94608, USA
    J Biol Chem 279:31357-64. 2004
  5. pmc Mechanism of the regulation of type IB phosphoinositide 3OH-kinase byG-protein betagamma subunits
    Sonja Krugmann
    Signalling Programme, The Babraham Institute, Babraham, Cambridge CB2 4AT, U K
    Biochem J 362:725-31. 2002
  6. ncbi Gbetagamma-mediated regulation of Golgi organization is through the direct activation of protein kinase D
    C Jamora
    Biology Department, University of California, San Diego, La Jolla 92093, USA
    Cell 98:59-68. 1999
  7. ncbi A novel kind of G protein heterodimer: the G beta5-RGS complex
    D Scott Witherow
    Department of Molecular and Cellular Pharmacology, University of Miami School of Medicine, Miami, Florida 33101, USA
    Receptors Channels 9:205-12. 2003
  8. pmc Saccharomyces cerevisiae cells execute a default pathway to select a mate in the absence of pheromone gradients
    R Dorer
    Department of Genetics, University of Washington, Seattle 98195 7360, USA
    J Cell Biol 131:845-61. 1995
  9. ncbi Nuclear localization of G protein beta 5 and regulator of G protein signaling 7 in neurons and brain
    J H Zhang
    Metabolic Diseases Branch and the Diabetes Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 276:10284-9. 2001
  10. ncbi Gbeta gamma isoforms selectively rescue plasma membrane localization and palmitoylation of mutant Galphas and Galphaq
    D S Evanko
    Department of Microbiology and Immunology and Kimmel Cancer Institute, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 276:23945-53. 2001

Research Grants

  1. Beta/Gamma Subunit Heterogeniety in G Proteins
    Janet D Robishaw; Fiscal Year: 2010
  2. G PROTEIN BETA GAMMA SIGNALING SYSTEMS IN VIVO
    JANET ROBISHAW; Fiscal Year: 2007
  3. Structural Studies of Galphaq and Its Complexes at the Cell Membrane
    JOHN TESMER; Fiscal Year: 2007
  4. Calcium channels at ribbon synapses in the retina
    CATHERINE MORGANS; Fiscal Year: 2007
  5. Activation of G Protein-Coupled Kinase 2
    JOHN TESMER; Fiscal Year: 2007
  6. The role of nyctalopin in the mammalian retina
    CATHERINE MORGANS; Fiscal Year: 2006
  7. EUKARYOTIC GENE REGULATION AS STUDIED IN YEAST
    Mark Ptashne; Fiscal Year: 2004
  8. Beta/Gamma Subunit Heterogeniety in G Proteins
    JANET ROBISHAW; Fiscal Year: 2004
  9. STRUCTURAL BASIS FOR BINDING TO G PROTEIN BETA GAMMA
    Alan Smrcka; Fiscal Year: 2007
  10. FASEB SUMMER CONFERENCE ON PHOSPHOLIPASES
    Suzanne Scarlata; Fiscal Year: 2007

Detail Information

Publications166 found, 100 shown here

  1. pmc Pheromone signalling in Saccharomyces cerevisiae requires the small GTP-binding protein Cdc42p and its activator CDC24
    Z S Zhao
    Institute of Molecular and Cell Biology, National University of Singapore, Kent Ridge
    Mol Cell Biol 15:5246-57. 1995
    ..The two-hybrid system revealed that Ste4p interacts with Cdc24p. We propose that Cdc42p plays a pivotal role both in polarization of the cytoskeleton and in pheromone signalling...
  2. ncbi Adenylyl cyclase type II domains involved in Gbetagamma stimulation
    S Weitmann
    , , Thielallee 67 73, 14195 Berlin, Germany
    Biochemistry 40:10853-8. 2001
    ....
  3. ncbi Absence of the RGS9.Gbeta5 GTPase-activating complex in photoreceptors of the R9AP knockout mouse
    Gabor Keresztes
    Department of Otolaryngology Eaton Peabody Laboratory, Harvard Medical School, Massachusetts Eye and Ear Infirmary, 243 Charles Street, Boston, MA 02114, USA
    J Biol Chem 279:1581-4. 2004
    ..Gbeta5 complex, and therefore all three proteins, RGS9, Gbeta5 , and R9AP, are obligate members of the regulatory complex that speeds the rate at which transducin hydrolyzes GTP...
  4. ncbi Spatial and temporal regulation of RACK1 function and N-methyl-D-aspartate receptor activity through WD40 motif-mediated dimerization
    Claire Thornton
    Ernest Gallo Research Center, Emeryville, California 94608, USA
    J Biol Chem 279:31357-64. 2004
    ..Based on these results, we propose that WD40-mediated homo- and heterodimerization of RACK1 mediate the formation of a transient signaling complex that includes the NMDAR, a G protein and Fyn...
  5. pmc Mechanism of the regulation of type IB phosphoinositide 3OH-kinase byG-protein betagamma subunits
    Sonja Krugmann
    Signalling Programme, The Babraham Institute, Babraham, Cambridge CB2 4AT, U K
    Biochem J 362:725-31. 2002
    ..We conclude that Gbetagammas activate type IB PI3K by a mechanism other than translocation to the plasma membrane...
  6. ncbi Gbetagamma-mediated regulation of Golgi organization is through the direct activation of protein kinase D
    C Jamora
    Biology Department, University of California, San Diego, La Jolla 92093, USA
    Cell 98:59-68. 1999
    ..Our findings suggest a possible mechanism by which the direct interaction of Gbetagamma with PKD regulates the dynamics of Golgi membranes and protein secretion...
  7. ncbi A novel kind of G protein heterodimer: the G beta5-RGS complex
    D Scott Witherow
    Department of Molecular and Cellular Pharmacology, University of Miami School of Medicine, Miami, Florida 33101, USA
    Receptors Channels 9:205-12. 2003
    ..This review summarizes the information about the assembly and function of G beta5-RGS dimers, as well as their posttranslational modifications and localization...
  8. pmc Saccharomyces cerevisiae cells execute a default pathway to select a mate in the absence of pheromone gradients
    R Dorer
    Department of Genetics, University of Washington, Seattle 98195 7360, USA
    J Cell Biol 131:845-61. 1995
    ..These observations reveal a molecular relationship between the mating and budding polarity pathways...
  9. ncbi Nuclear localization of G protein beta 5 and regulator of G protein signaling 7 in neurons and brain
    J H Zhang
    Metabolic Diseases Branch and the Diabetes Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 276:10284-9. 2001
    ..RGS protein complexes suggests a potential role in neurons communicating between classical heterotrimeric G protein subunits and/or their effectors at the plasma membrane and the cell nucleus...
  10. ncbi Gbeta gamma isoforms selectively rescue plasma membrane localization and palmitoylation of mutant Galphas and Galphaq
    D S Evanko
    Department of Microbiology and Immunology and Kimmel Cancer Institute, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 276:23945-53. 2001
    ....
  11. ncbi G protein betagamma directly regulates SNARE protein fusion machinery for secretory granule exocytosis
    Trillium Blackmer
    Department of Biological Sciences, University of Illinois at Chicago, 840 West Taylor Street, Chicago, Illinois 60607, USA
    Nat Neurosci 8:421-5. 2005
    ..Here we show inhibitory coupling between GPCRs and vesicle exocytosis mediated directly by Gbetagamma interactions with the Ca(2+)-dependent fusion machinery...
  12. ncbi Expression levels of RGS7 and RGS4 proteins determine the mode of regulation of the G protein-activated K(+) channel and control regulation of RGS7 by G beta 5
    T Keren-Raifman
    Department of Physiology and Pharmacology, Sackler School of Medicine, Tel Aviv University, Ramat Aviv, Israel
    FEBS Lett 492:20-8. 2001
    ..These dual effects resolve previous controversies regarding RGS4 and RGS7 function and indicate that they modulate signaling by mechanisms supplementary to their GTPase-activating protein activity...
  13. pmc Crystal structure of the multifunctional Gbeta5-RGS9 complex
    Matthew L Cheever
    Department of Pharmacology, University of North Carolina School of Medicine, Campus Box 7365, Chapel Hill, North Carolina 27599 7365, USA
    Nat Struct Mol Biol 15:155-62. 2008
    ..This structure reveals a canonical RGS domain that is functionally integrated within a molecular complex that is poised for integration of multiple steps during G-protein activation and deactivation...
  14. ncbi Interaction of Gbetagamma with RACK1 and other WD40 repeat proteins
    Songhai Chen
    Department of Pharmacology, Vanderbilt University Medical Center, Room 442, Robinson Research Building, Nashville, TN 37232, USA
    J Mol Cell Cardiol 37:399-406. 2004
    ..Here we will describe the molecular mechanism underlying this interaction and the implications of the interaction on the signal transduction of G-protein and RACK1...
  15. pmc RGS9-2 modulates D2 dopamine receptor-mediated Ca2+ channel inhibition in rat striatal cholinergic interneurons
    Theresa M Cabrera-Vera
    Institute for Neuroscience and Department of Molecular Pharmacology and Biological Chemistry, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
    Proc Natl Acad Sci U S A 101:16339-44. 2004
    ..These results implicate RGS9-2 as a specific regulator of dopamine receptor-mediated signaling in the striatum and identify a role for GAP activity modulation by the DEP-GGL domain...
  16. ncbi Distinct roles for Galpha and Gbetagamma in regulating spindle position and orientation in Caenorhabditis elegans embryos
    M Gotta
    Wellcome CRC Institute, Tennis Court Road, Cambridge, CB2 1QR, UK
    Nat Cell Biol 3:297-300. 2001
    ..Gbetagamma is important in regulating migration of the centrosome around the nucleus and hence in orientating the mitotic spindle. Galpha is required for asymmetric spindle positioning in the one-celled embryo...
  17. ncbi G protein betagamma subunit-mediated presynaptic inhibition: regulation of exocytotic fusion downstream of Ca2+ entry
    T Blackmer
    Department of Molecular Pharmacology and Biological Chemistry, Department of Physiology, Northwestern University Institute for Neuroscience, Northwestern University Medical School, 303 East Chicago Avenue, Chicago, IL 60611, USA
    Science 292:293-7. 2001
    ..Thus, Gbetagamma blocked neurotransmitter release downstream of Ca2+ entry and may directly target the exocytotic fusion machinery at the presynaptic terminal...
  18. ncbi Regulation of G protein-initiated signal transduction in yeast: paradigms and principles
    H G Dohlman
    Department of Pharmacology, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, Connecticut 06536 0812, USA
    Annu Rev Biochem 70:703-54. 2001
    ....
  19. ncbi RACK1 regulates specific functions of Gbetagamma
    Songhai Chen
    Department of Pharmacology, Vanderbilt University Medical Center, Nashville, Tennessee 37232 6600, USA
    J Biol Chem 279:17861-8. 2004
    ..Similarly, RACK1 does not affect signal transduction through the Galpha subunits of G(i), G(s), or G(q). Collectively, these findings suggest a role of RACK1 in regulating specific functions of Gbetagamma...
  20. ncbi The role of electrostatic interactions in the regulation of the membrane association of G protein beta gamma heterodimers
    D Murray
    Department of Biochemistry, Howard Hughes Medical Institute, Columbia University, New York, New York 10032, USA
    J Biol Chem 276:45153-9. 2001
    ..Sequence analysis and homology model building suggest that our conclusions may be generalized to other Gbetagamma and phosducin isoforms as well...
  21. pmc G-protein beta subunit of Cochliobolus heterostrophus involved in virulence, asexual and sexual reproductive ability, and morphogenesis
    Sherif Ganem
    Department of Biology, Technion, Haifa 32000, Israel
    Eukaryot Cell 3:1653-63. 2004
    ..These data may be genetic evidence for a novel cell death-related function of the Gbeta subunit in filamentous fungi...
  22. ncbi Gbetagamma subunits stimulate p21-activated kinase 1 (PAK1) through activation of PI3-kinase and Akt but act independently of Rac1/Cdc42
    Raymond E Menard
    Department of Pharmacology, Wayne State University, 540 East Canfield Avenue, Room 6326, Detroit, MI 48201, USA
    FEBS Lett 556:187-92. 2004
    ..These results reveal that stimulation of PAK1 by Gbetagamma can occur via a PI3-kinase and Akt pathway that does not require Rac1 or Cdc42...
  23. ncbi The G-beta subunit MGB1 is involved in regulating multiple steps of infection-related morphogenesis in Magnaporthe grisea
    Marie Nishimura
    National Institute of Agrobiological Sciences, 2 1 2, Kan non dai, Tsukuba, Ibaraki, 305 8602, Japan
    Mol Microbiol 50:231-43. 2003
    ..The Pmk1 pathway may be the downstream target of MGB1 for regulating penetration and infectious hyphae growth in M. grisea...
  24. ncbi Gbetagamma-mediated prostacyclin production and cAMP-dependent protein kinase activation by endothelin-1 promotes vascular smooth muscle cell hypertrophy through inhibition of glycogen synthase kinase-3
    Sebastien Taurin
    Department of Medicine, University of Chicago, Chicago, Illinois 60637, USA
    J Biol Chem 282:19518-25. 2007
    ....
  25. ncbi Characterizing the interactions between the two subunits of the p101/p110gamma phosphoinositide 3-kinase and their role in the activation of this enzyme by G beta gamma subunits
    S Krugmann
    Signalling Programme, The Babraham Institute, Babraham, Cambridge CB2 4AT, United Kingdom
    J Biol Chem 274:17152-8. 1999
    ..While modifications of the N terminus of p110gamma could modulate its intrinsic catalytic activity, binding to the N-terminal region of p101 was found to be indispensable for activation of heterodimers with Gbetagamma...
  26. ncbi The role of Far1p in linking the heterotrimeric G protein to polarity establishment proteins during yeast mating
    A C Butty
    Swiss Institute for Experimental Cancer Research ISREC, Chemin des Boveresses 155, 1066 Epalinges VD, Switzerland
    Science 282:1511-6. 1998
    ..Thus, Far1p functions as an adaptor that recruits polarity establishment proteins to the site of extracellular signaling marked by Gbetagamma to polarize assembly of the cytoskeleton in a morphogenetic gradient...
  27. ncbi Regulation of membrane and subunit interactions by N-myristoylation of a G protein alpha subunit in yeast
    J Song
    Department of Pharmacology, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, Connecticut 06536 0812, USA
    J Biol Chem 271:20273-83. 1996
    ..These data suggest that myristoylation is required for specific targeting of Gpa1p to the plasma membrane, where it is needed to interact with the receptor and to regulate the release of Gbetagamma...
  28. ncbi Interaction of a G-protein beta-subunit with a conserved sequence in Ste20/PAK family protein kinases
    T Leeuw
    Eukaryotic Genetics Group, Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec
    Nature 391:191-5. 1998
    ....
  29. ncbi Fusarium oxysporum G-protein beta subunit Fgb1 regulates hyphal growth, development, and virulence through multiple signalling pathways
    Jesús Delgado-Jarana
    Departamento de Genetica, Universidad de Cordoba, Campus Universitario de Rabanales Edif C5, 14071 Cordoba, Spain
    Fungal Genet Biol 42:61-72. 2005
    ..Our results support a model in which Fgb1 controls hyphal growth, development and virulence in F. oxysporum both through cAMP-dependent and -independent pathways...
  30. ncbi Distinct sites on G protein beta gamma subunits regulate different effector functions
    Tooraj Mirshahi
    Department of Physiology and Biophysics, Mount Sinai School of Medicine of New York University, New York, New York 10029, USA
    J Biol Chem 277:36345-50. 2002
    ..These data show that distinct domains within Gbetagamma subserve specific functional roles...
  31. ncbi The G protein beta subunit FGB1 regulates development and pathogenicity in Fusarium oxysporum
    Sona Jain
    Department of Applied Life Science, Faculty of Agriculture, Ehime University, 790 8566 Matsuyama, Japan
    Curr Genet 43:79-86. 2003
    ..oxysporum. These results suggest that FGA1 and FGB1 have partially overlapping functions in the regulation of development and pathogenicity in F. oxysporum...
  32. ncbi Prolonged photoresponses and defective adaptation in rods of Gbeta5-/- mice
    Claudia M Krispel
    Center for Neuroscience, University of California, Davis, Davis, California 95616, USA
    J Neurosci 23:6965-71. 2003
    ..In addition, our light adaptation studies support the notion than an additional weak GTPase-accelerating factor in rods is regulated by intracellular calcium and/or cGMP...
  33. ncbi G Protein betagamma subunits stimulate p114RhoGEF, a guanine nucleotide exchange factor for RhoA and Rac1: regulation of cell shape and reactive oxygen species production
    Jiaxin Niu
    Department of Pharmacology, College of Medicine, University of Illinois at Chicago, Chicago, Ill 60612, USA
    Circ Res 93:848-56. 2003
    ..The findings help to explain the integrated effects of LPA and other G-protein receptor-coupled agonists on actin stress fiber formation, cell shape change, and ROS production...
  34. ncbi A fifth member of the mammalian G-protein beta-subunit family. Expression in brain and activation of the beta 2 isotype of phospholipase C
    A J Watson
    Division of Biology, California Institute of Technology, Pasadena 91125
    J Biol Chem 269:22150-6. 1994
    ..The rather low level of identity between G beta 5 and the other mammalian G beta-subunits may shed light on structural/sequence elements necessary for G beta protein function...
  35. ncbi R7BP augments the function of RGS7*Gbeta5 complexes by a plasma membrane-targeting mechanism
    Ryan M Drenan
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 281:28222-31. 2006
    ..Therefore, R7BP augments the function of the complex by a palmitoylation-regulated plasma membrane-targeting mechanism...
  36. ncbi Interaction sites of the G protein beta subunit with brain G protein-coupled inward rectifier K+ channel
    A M Albsoul Younes
    Department of Pharmacology, University of Illinois, Chicago, Illinois 60612, USA
    J Biol Chem 276:12712-7. 2001
    ....
  37. ncbi RGS7 is palmitoylated and exists as biochemically distinct forms
    J J Rose
    Department of Pharmacology, Emory University School of Medicine, Atlanta, Georgia 30322 3090, USA
    J Neurochem 75:2103-12. 2000
    ..These findings define previously unrecognized biochemical properties of RGS7, including the first demonstration that RGS7 is palmitoylated...
  38. ncbi Gbeta gamma mediate differentiation of vascular smooth muscle cells
    H P Reusch
    Institute of Clinical Pharmacology and Toxicology and the Institute of Pharmacology, Benjamin Franklin Medical Center, , Berlin 14195, Germany
    J Biol Chem 276:19540-7. 2001
    ..We conclude that receptor-mediated differentiation of VSM cells requires Gbetagamma and an intact Ras/Raf/MEK/ERK signaling...
  39. ncbi Gbeta residues that do not interact with Galpha underlie agonist-independent activity of K+ channels
    Tooraj Mirshahi
    Department of Physiology and Biophysics, Mt Sinai School of Medicine of NYU, New York, NY 10029, USA
    J Biol Chem 277:7348-55. 2002
    ..Our findings indicate that the presence of Gbeta residues that do not interact with Galpha are involved in Gbetagamma interactions in the absence of agonist stimulation...
  40. ncbi Brain-specific RGS9-2 is localized to the nucleus via its unique proline-rich domain
    Mohamad Bouhamdan
    Department of Psychiatry, Wayne State University School of Medicine, 540 E Canfield, 2309 Scott Hall, Detroit, MI 48201, USA
    Biochim Biophys Acta 1691:141-50. 2004
    ..The nuclear localization of RGS9-2 suggests a heretofore-unanticipated role for this brain-specific protein in transducing signals to the nuclei of forebrain neurons...
  41. ncbi The G beta gamma complex of the yeast pheromone response pathway. Subcellular fractionation and protein-protein interactions
    J E Hirschman
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester 01655 0122, USA
    J Biol Chem 272:240-8. 1997
    ..These results indicate that at least 40% of Ste4p and Ste18p are part of a G betagamma complex at the plasma membrane and that stable association of this complex with the plasma membrane requires the presence of G alpha...
  42. ncbi G beta 5.RGS7 inhibits G alpha q-mediated signaling via a direct protein-protein interaction
    D Scott Witherow
    Department of Molecular and Cellular Pharmacology and the Neuroscience Program, University of Miami School of Medicine, Miami, Florida 33136, USA
    J Biol Chem 278:21307-13. 2003
    ..Gbeta5 complex and cyan fluorescence protein-tagged Galphaq, indicating a direct interaction between these molecules...
  43. pmc Evidence that mating by the Saccharomyces cerevisiae gpa1Val50 mutant occurs through the default mating pathway and a suggestion of a role for ubiquitin-mediated proteolysis
    B e Xu
    Department of Microbiology and Molecular Genetics, University of Vermont, College of Medicine, Burlington, USA
    Mol Biol Cell 8:1649-64. 1997
    ..On the basis of these results, we suggest that a positive component of the default mating pathway is subject to ubiquitin-mediated degradation...
  44. ncbi Translating G protein subunit diversity into functional specificity
    Janet D Robishaw
    100 N Academy Ave, Weis Center for Research, Geisinger Clinic, Danville, PA 17822 2614, USA
    Curr Opin Cell Biol 16:206-9. 2004
    ..Although much remains to be learned, the assembly of specific alphabetagamma subunit combinations seems to involve both structural and spatial factors...
  45. ncbi betagamma subunits of heterotrimeric G-proteins contribute to Ca2+ release at fertilization in the sea urchin
    Ekaterina Voronina
    Department of Molecular and Cell Biology and Biochemistry, Brown University, 69 Brown Street, Providence, RI 02912, USA
    J Cell Sci 117:5995-6005. 2004
    ..We propose integration of the G-protein input into the framework of signaling at sea urchin fertilization...
  46. pmc Submembraneous microtubule cytoskeleton: regulation of microtubule assembly by heterotrimeric Gproteins
    Sukla Roychowdhury
    Department of Biological Sciences, University of Texas, El Paso, TX, USA
    FEBS J 275:4654-63. 2008
    ....
  47. ncbi Activation of serum response element by D2 dopamine receptor is governed by Gbetagamma-mediated MAPK and Rho pathways and regulated by RGS proteins
    Guyu Ho
    Wyeth Neuroscience Research, Wyeth Research, Princeton, N J, USA
    Pharmacology 79:114-21. 2007
    ..These results delineate the signaling pathways which couple D2 receptor to the transcriptional activation of SRE and demonstrate a modulatory role for RGS proteins in these processes...
  48. ncbi Gbetagamma inhibits Galpha GTPase-activating proteins by inhibition of Galpha-GTP binding during stimulation by receptor
    Wei Tang
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9041, USA
    J Biol Chem 281:4746-53. 2006
    ....
  49. ncbi Influence of membrane components in the binding of proteins to membrane surfaces
    Finly Philip
    Department of Physiology and Biophysics, State University of New York at Stony Brook, Stony Brook, New York 11794 8661, USA
    Biochemistry 43:11691-700. 2004
    ..For these proteins, the formation of larger protein complexes with very high membrane affinities is unlikely...
  50. ncbi Purification and in vitro functional analysis of the Arabidopsis thaliana regulator of G-protein signaling-1
    Francis S Willard
    Department of Pharmacology, The University of North Carolina at Chapel Hill, 27599 7365, USA
    Methods Enzymol 389:320-38. 2004
    ..This article describes methods for the purification and in vitro functional analysis of the RGS box of AtRGS1...
  51. pmc Expansion of signal transduction by G proteins. The second 15 years or so: from 3 to 16 alpha subunits plus betagamma dimers
    Lutz Birnbaumer
    Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, NIH, DHHS, Research Triangle Park, NC 27709, USA
    Biochim Biophys Acta 1768:772-93. 2007
    ..Emphasis is placed in presenting how the field developed with the hope of conveying why many of the new findings were made...
  52. ncbi The Gbetagamma dimer drives the interaction of heterotrimeric Gi proteins with nonlamellar membrane structures
    Oliver Vögler
    Institut Universitari d Investigacio en Ciencies de la Salut, Department of Biology, University of the Balearic Islands, Carretera Valldemossa Kilómetro 7 5, E 07122 Palma de Mallorca, Spain
    J Biol Chem 279:36540-5. 2004
    ..Finally, their differential interactions with lipids suggest an active role of the membrane lipid secondary structure in the propagation of signals through G protein-coupled receptors...
  53. ncbi Pharmacology. Hitting the hot spots of cell signaling cascades
    John Joseph Grubb Tesmer
    Department of Pharmacology, Life Sciences Institute, University of Michigan, 210 Washtenaw Avenue, Ann Arbor, MI 48109, USA
    Science 312:377-8. 2006
  54. ncbi Over-expression of a truncated Arabidopsis thaliana heterotrimeric G protein gamma subunit results in a phenotype similar to alpha and beta subunit knockouts
    David Chakravorty
    University of Queensland Brisbane, Queensland, 4072, Australia
    Gene 393:163-70. 2007
    ....
  55. pmc Heterotrimeric G protein signaling in the Arabidopsis unfolded protein response
    Shiyu Wang
    Biology Department and Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA 16802, USA
    Proc Natl Acad Sci U S A 104:3817-22. 2007
    ....
  56. ncbi A Purkinje cell specific GoLoco domain protein, L7/Pcp-2, modulates receptor-mediated inhibition of Cav2.1 Ca2+ channels in a dose-dependent manner
    Mariko Kinoshita-Kawada
    Department of Neuroscience and the Center for Molecular Neurobiology, The Ohio State University, 168 Rightmire Hall, 1060 Carmack Road, Columbus, OH 43210, USA
    Brain Res Mol Brain Res 132:73-86. 2004
    ..This provides a framework for designing further experiments to determine how dendritic local fluctuations in L7 protein levels might influence signal processing in Purkinje cells...
  57. ncbi Differential targeting of Gbetagamma-subunit signaling with small molecules
    Tabetha M Bonacci
    Department of Pharmacology and Physiology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    Science 312:443-6. 2006
    ..These data demonstrate an approach for modulation of G protein-coupled receptor signaling that may represent an important therapeutic strategy...
  58. ncbi Specificity of Gbetagamma signaling to Kir3 channels depends on the helical domain of pertussis toxin-sensitive Galpha subunits
    Radda Rusinova
    Department of Structural and Chemical Biology, Mount Sinai School of Medicine, New York, NY 10029, USA
    J Biol Chem 282:34019-30. 2007
    ..These results implicate the helical domain of Galpha(i) proteins as a critical determinant of Gbetagamma signaling specificity...
  59. ncbi Selective interactions between G protein subunits and RGS4 with the C-terminal domains of the mu- and delta-opioid receptors regulate opioid receptor signaling
    Zafiroula Georgoussi
    Laboratory of Cellular Signaling and Molecular Pharmacology, Institute of Biology, National Center for Scientific Research Demokritos Ag Paraskevi 15310, Athens, Greece
    Cell Signal 18:771-82. 2006
    ..Collectively, our data demonstrate that the C-termini of the mu- and delta-ORs provide direct physical scaffolding in which G protein subunits and RGS4 protein can be bound...
  60. pmc Galpha Gbetagamma dissociation may be due to retraction of a buried lysine and disruption of an aromatic cluster by a GTP-sensing Arg Trp pair
    Andrew F Neuwald
    J Craig Venter Institute, Rockville, MD 20850, USA
    Protein Sci 16:2570-7. 2007
    ..Other structural features associated with Galpha functional divergence further support the proposed mechanism...
  61. ncbi Ultra-low-dose naloxone suppresses opioid tolerance, dependence and associated changes in mu opioid receptor-G protein coupling and Gbetagamma signaling
    H Y Wang
    Department of Physiology and Pharmacology, City University of New York Medical School, 138th Street and Convent Avenue, New York, NY 10031, USA
    Neuroscience 135:247-61. 2005
    ..These findings provide a molecular mechanism underpinning opioid tolerance and dependence and their attenuation by ultra-low-dose opioid antagonists...
  62. ncbi G proteins Go green: a plant G protein signaling FAQ sheet
    Sarah M Assmann
    Biology Department, Pennsylvania State University, 208 Mueller Laboratory, University Park, PA 16802, USA
    Science 310:71-3. 2005
    ..Pathways in Science's Signal Transduction Knowledge Environment Connections Maps database provide details about the emerging roles of G proteins in several cellular processes of plants...
  63. pmc Heterotrimeric G proteins facilitate Arabidopsis resistance to necrotrophic pathogens and are involved in jasmonate signaling
    Yuri Trusov
    Plant Genetic Engineering Laboratory, Department of Botany, School of Integrative Biology, University of Queensland, Brisbane, Queensland 4072, Australia
    Plant Physiol 140:210-20. 2006
    ..We hypothesize that Gbetagamma acts as a direct or indirect enhancer of the jasmonate signaling pathway in plants...
  64. ncbi Loss of association between activated Galpha q and Gbetagamma disrupts receptor-dependent and receptor-independent signaling
    Daniel S Evanko
    Department of Microbiology and Immunology, Kimmel Cancer Center, Thomas Jefferson University, 233 S 10th St, 839 BLSB, Philadelphia, PA 19107, USA
    Cell Signal 17:1218-28. 2005
    ..Taken together, results presented here suggest that alpha-betagamma binding is necessary at a point downstream from receptor activation of the heterotrimeric G protein for signal transduction by alpha q...
  65. pmc The heterotrimeric G-protein GanB(alpha)-SfaD(beta)-GpgA(gamma) is a carbon source sensor involved in early cAMP-dependent germination in Aspergillus nidulans
    Anne Lafon
    Unité Postulante Biologie et Pathogénicité Fongiques, INRA USC2019, Institut Pasteur, Paris, France
    Genetics 171:71-80. 2005
    ..Furthermore, GanB may function in sensing various carbon sources and subsequent activation of downstream signaling for germination...
  66. pmc The modulator of nongenomic actions of the estrogen receptor (MNAR) regulates transcription-independent androgen receptor-mediated signaling: evidence that MNAR participates in G protein-regulated meiosis in Xenopus laevis oocytes
    Derek Haas
    Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390 8857, USA
    Mol Endocrinol 19:2035-46. 2005
    ..Androgen binding to AR might then release this inhibition, allowing maturation to occur. Thus, MNAR may augment multiple nongenomic signals, depending upon the context and cell type in which it is expressed...
  67. pmc Heterotrimeric G-proteins: a short history
    Graeme Milligan
    Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ
    Br J Pharmacol 147:S46-55. 2006
    ..Finally, further modifications of such chimeras have generated a range of G-protein alpha-subunits with greater promiscuity to interact across GPCR classes and initiated the use of such modified G-proteins in drug discovery programmes...
  68. pmc Determination of the activation volume of PLCbeta by Gbeta gamma-subunits through the use of high hydrostatic pressure
    Suzanne Scarlata
    Department of Physiology and Biophysics, State University of New York, Stony Brook, New York 11794 8661, USA
    Biophys J 88:2867-74. 2005
    ..These results suggest that activation of PLCbeta by Gbeta gamma is conferred by a small (i.e., 3-15 ml/mol) volume element...
  69. pmc Assembly and trafficking of heterotrimeric G proteins
    Yannick Marrari
    Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Biochemistry 46:7665-77. 2007
    ..Understanding G protein assembly and trafficking will lead to a greater understanding of novel ways that cells regulate G protein signaling...
  70. pmc Distinct roles for two Galpha-Gbeta interfaces in cell polarity control by a yeast heterotrimeric G protein
    Shelly C Strickfaden
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Mol Biol Cell 19:181-97. 2008
    ..These findings raise the possibility that the Galphabetagamma heterotrimer can function in a partially dissociated state, tethered by the N-terminal interface...
  71. ncbi Anticancer activity of BIM-46174, a new inhibitor of the heterotrimeric Galpha/Gbetagamma protein complex
    Gregoire P Prevost
    Ipsen Institut Henri Beaufour, Les Ulis, France
    Cancer Res 66:9227-34. 2006
    ..Our data validate the feasibility of targeting heterotrimeric G-protein functions downstream the GPCRs to improve anticancer chemotherapy...
  72. ncbi G protein signaling in yeast: new components, new connections, new compartments
    Janna E Slessareva
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599 7260, USA
    Science 314:1412-3. 2006
    ..New tools available in yeast are beginning to uncover new pathway components and interactions and have revealed signaling in unexpected locations within the cell...
  73. ncbi Association of the yeast pheromone response G protein beta gamma subunits with the MAP kinase scaffold Ste5p
    M S Whiteway
    Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec
    Science 269:1572-5. 1995
    ..Thus, association of the G protein and the MAP kinase cassette via the scaffolding protein Ste5p may transmit the G protein signal...
  74. ncbi G beta gamma mediates the interplay between tubulin dimers and microtubules in the modulation of Gq signaling
    Juliana S Popova
    Department of Physiology and Biophysics, College of Medicine, University of Illinois, Chicago, Illinois 60612 7342, USA
    J Biol Chem 278:34299-308. 2003
    ..Thus G protein-coupled receptors might evoke G alpha and G beta gamma to orchestrate regulation of phospholipase signaling by tubulin dimers and control of cell shape by microtubules...
  75. pmc A Cdc24p-Far1p-Gbetagamma protein complex required for yeast orientation during mating
    A Nern
    Division of Cell Biology, MRC Laboratory of Molecular Biology, Cambridge, CB2 2QH, United Kingdom
    J Cell Biol 144:1187-202. 1999
    ..These results suggest that formation of a Cdc24p-Far1p-Gbetagamma complex functions as a landmark for orientation of the cytoskeleton during growth towards an external signal...
  76. pmc Roles of G beta gamma in membrane recruitment and activation of p110 gamma/p101 phosphoinositide 3-kinase gamma
    Carsten Brock
    Institut für Physiologische Chemie II, Klinikum der Heinrich Heine Universität, 40225 Dusseldorf, Germany
    J Cell Biol 160:89-99. 2003
    ..e., membrane recruitment is mediated via the noncatalytic p101 subunit, and direct stimulation of G beta gamma with p110 gamma contributes to activation of PI3K gamma...
  77. pmc Gbeta gamma -independent constitutive association of Galpha s with SHP-1 and angiotensin II receptor AT2 is essential in AT2-mediated ITIM-independent activation of SHP-1
    Ying Hong Feng
    Department of Medicine, Case Western Reserve University School of Medicine, and University Hospital of Cleveland, 10900 Euclid Avenue, Cleveland, OH 44106 4982, USA
    Proc Natl Acad Sci U S A 99:12049-54. 2002
    ..The AT(2)-mediated ITIM-independent activation of SHP-1 that is distinct from the conventional mode of activation, may represent a general paradigm for activation of SHP-1/2-class tyrosine phosphatases by G protein-coupled receptors...
  78. pmc The rat cytomegalovirus R33-encoded G protein-coupled receptor signals in a constitutive fashion
    Yvonne K Gruijthuijsen
    Department of Medical Microbiology, Cardiovascular Research Institute Maastricht, University of Maastricht, 6202 AZ Maastricht, The Netherlands
    J Virol 76:1328-38. 2002
    ..In contrast to its homolog of human herpesvirus 6B (pU12), pR33 does not bind RANTES...
  79. ncbi Coupling of the insulin-like growth factor-I receptor tyrosine kinase to Gi2 in human intestinal smooth muscle: Gbetagamma -dependent mitogen-activated protein kinase activation and growth
    J F Kuemmerle
    Departments of Medicine and Physiology, Medical College of Virginia of Virginia Commonwealth University, Richmond, Virginia 23298, USA
    J Biol Chem 276:7187-94. 2001
    ....
  80. ncbi Inhibition of GDP/GTP exchange on G alpha subunits by proteins containing G-protein regulatory motifs
    M Natochin
    Department of Physiology and Biophysics, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
    Biochemistry 40:5322-8. 2001
    ..Our results suggest that GPR proteins are potent GDP dissociation inhibitors for Gialpha-like Galpha subunits in vitro, and in this capacity they may inhibit GPCR/Gi protein signaling in vivo...
  81. ncbi A function for phosphoinositide 3-kinase beta lipid products in coupling beta gamma to Ras activation in response to lysophosphatidic acid
    Armelle Yart
    INSERM U326, IFR 30, Hopital Purpan, Toulouse 31059, France
    J Biol Chem 277:21167-78. 2002
    ..e. Gbetagamma release and tyrosine kinase receptor transactivation...
  82. ncbi Role of the G protein gamma subunit in beta gamma complex modulation of phospholipase Cbeta function
    Muslum Akgoz
    Department of Anesthesiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 277:19573-8. 2002
    ..All mutants, however, interacted equally effectively with the G(o)alpha subunit. The results indicate that the G protein gamma subunit plays a direct role in the modulation of effector function by the betagamma complex...
  83. ncbi Biochemical analysis of yeast G(alpha) mutants that enhance adaptation to pheromone
    M J Cismowski
    Laboratory for Molecular Biology, University of Illinois at Chicago, Chicago, Illinois 60607, USA
    Biochem Biophys Res Commun 284:247-54. 2001
    ..A combination of G(alpha) affinity chromatography, GTP binding/hydrolysis studies, and genetic analysis allowed us to assign a distinct mechanism of action to each of these mutant proteins...
  84. ncbi G protein specificity: traffic direction required
    Paul R Albert
    Ottawa Health Research Institute, Neuroscience, University of Ottawa, 451 Smyth Road, K1H 8M5, Ottawa, ON, Canada
    Cell Signal 14:407-18. 2002
    ..By identifying G protein specificity for individual receptor signaling pathways, ligands targeted to disrupt individual pathways of a given receptor could be developed...
  85. ncbi G beta gamma counteracts G alpha(q) signaling upon alpha(1)-adrenergic receptor stimulation
    Motohiro Nishida
    Laboratory of Pharmacology and Toxicology, Graduate School of Pharmaceutical Science, University of Tokyo, Tokyo 113 0033, Japan
    Biochem Biophys Res Commun 291:995-1000. 2002
    ..However, activation of Akt but not ERK plays an important role in the protection against the G alpha(q)-induced cellular injury and apoptosis...
  86. ncbi Multiple pathways of ERK activation by G protein-coupled receptors
    T Gudermann
    , , Germany
    Novartis Found Symp 239:68-79; discussion 80-4, 150-9. 2001
    ....
  87. pmc Mapping of a yeast G protein betagamma signaling interaction
    S J Dowell
    Glaxo Wellcome Research and Development, Stevenage, SG1 2NY, United Kingdom
    Genetics 150:1407-17. 1998
    ..Identification of the Gbetagamma coiled-coil in Ste5p binding may set a precedent for Gbetagamma-effector interactions in more complex organisms...
  88. pmc Ggamma in dictyostelium: its role in localization of gbetagamma to the membrane is required for chemotaxis in shallow gradients
    N Zhang
    Department of Cell Biology and Anatomy, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Mol Biol Cell 12:3204-13. 2001
    ..Thus, a full complement of membrane-tethered Gbetagamma is required for sensing shallow gradients, but is not essential for responses to increments in extracellular stimuli...
  89. ncbi Suppression of a dominant G-protein beta-subunit mutation in yeast by G alpha protein expression
    M Zhang
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical Center, Worcester 01655
    Mol Microbiol 9:813-21. 1993
    ..A G alpha i hybrid also restores growth and allows inefficient mating in the STE4HPL strain...
  90. ncbi Yeast assays for G-protein-coupled receptors
    S J Dowell
    GlaxoSmithKline, Medicines Research Centre, Gunnels Wood Road, Stevenage, Hertfordshire, SG1 2NY, UK
    Receptors Channels 8:343-52. 2002
    ..Yeast genetics has also been applied to a functional analysis of GPCRs and peptide ligands. In this review we describe the historical development of yeast GPCR assay systems and their current applications...
  91. ncbi Activation of nuclear factor {kappa}B by somatostatin type 2 receptor in pancreatic acinar AR42J cells involves G{alpha}14 and multiple signaling components: a mechanism requiring protein kinase C, calmodulin-dependent kinase II, ERK, and c-Src
    Andrew M F Liu
    Department of Biochemistry, Molecular Neuroscience Center, and Biotechnology Research Institute, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    J Biol Chem 280:34617-25. 2005
    ..Similar results were also obtained using AR42J cells. These data suggest that activation of the IKK/NFkappaB signaling cascade by SSTR2 requires a complicated network consisting of Galpha(14) and multiple intermediates...
  92. ncbi Alpha-thrombin-mediated phosphatidylinositol 3-kinase activation through release of Gbetagamma dimers from Galphaq and Galphai2
    Reema Goel
    Department of Pharmacological and Physiological Sciences, St Louis University Medical School, St Louis, Missouri 63104, USA
    J Biol Chem 279:6701-10. 2004
    ....
  93. ncbi Assay for G protein-dependent activation of phospholipase C beta using purified protein components
    Mousumi Ghosh
    Department of Pharmacology and Physiology, University of Rochester School of Medicine, NY, USA
    Methods Mol Biol 237:67-75. 2004
    ..It can also be used to assess the functionality of the components after modification by mutagenesis, chemical modification, or in the presence of competing molecules...
  94. pmc Genetic identification of residues involved in association of alpha and beta G-protein subunits
    M Whiteway
    National Research Council, Biotechnology Research Institute, Montreal, Quebec, Canada
    Mol Cell Biol 14:3223-9. 1994
    ..Because the ability of the modified G alpha subunit to suppress the Ste4 mutations is allele specific, it is likely that the residues defined by this analysis play a direct role in G-protein subunit association...
  95. ncbi Galphai1 and Galphai3 differentially interact with, and regulate, the G protein-activated K+ channel
    Tatiana Ivanina
    Department of Physiology and Pharmacology, Sackler School of Medicine, Tel Aviv University, Ramat Aviv 69978, Israel
    J Biol Chem 279:17260-8. 2004
    ....
  96. ncbi Purification of G protein subunits from Sf9 insect cells using hexahistidine-tagged alpha and beta gamma subunits
    Tohru Kozasa
    Department of Pharmacology, University of Illinois at Chicago, USA
    Methods Mol Biol 237:21-38. 2004
    ..Using Sf9-Baculovirus expression system, a general and simplified method to purify various G protein subunits is described in this chapter. This method is useful for purification of most of G protein subunits...
  97. ncbi Differential distribution of the G protein gamma3 subunit in the developing zebrafish nervous system
    G M Kelly
    Department of Zoology, Molecular Genetics Unit, University of Western Ontario, ON, N6A 5B7, London, Canada
    Int J Dev Neurosci 19:455-67. 2001
    ..Together, these data imply that a betagamma heterodimer plays a role in signal transduction events involving G protein coupled receptors and that these events occur in specific regions in the nervous system of the developing zebrafish...
  98. pmc AKR1 encodes a candidate effector of the G beta gamma complex in the Saccharomyces cerevisiae pheromone response pathway and contributes to control of both cell shape and signal transduction
    P M Pryciak
    Department of Genetics, University of Washington, Seattle 98195 7360, USA
    Mol Cell Biol 16:2614-26. 1996
    ....
  99. ncbi Gbetagammas and the Ras binding domain of p110gamma are both important regulators of PI(3)Kgamma signalling in neutrophils
    Sabine Suire
    The Babraham Institute, Babraham, Cambs CB22 3AT, UK
    Nat Cell Biol 8:1303-9. 2006
    ..We conclude that Gbetagammas, p101 and the Ras-RBD interaction all have important roles in the regulation of PI(3)Kgamma in vivo and that they can simultaneously, but differentially, control distinct PI(3)Kgamma effectors...
  100. pmc Membrane anchoring subunits specify selective regulation of RGS9·Gbeta5 GAP complex in photoreceptor neurons
    Yan Cao
    Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota 55455, USA
    J Neurosci 30:13784-93. 2010
    ..These findings illustrate the diversity of the G-protein signaling regulation by RGS·Gβ5 complexes achieved by differential recruitment of the membrane anchors...
  101. pmc Loss of sustained Fus3p kinase activity and the G1 arrest response in cells expressing an inappropriate pheromone receptor
    A Couve
    Department of Cell Biology and Anatomy, Mount Sinai School of Medicine, New York, New York 10029, USA
    Mol Cell Biol 16:4478-85. 1996
    ....

Research Grants92

  1. Beta/Gamma Subunit Heterogeniety in G Proteins
    Janet D Robishaw; Fiscal Year: 2010
    ....
  2. G PROTEIN BETA GAMMA SIGNALING SYSTEMS IN VIVO
    JANET ROBISHAW; Fiscal Year: 2007
    ..Since many diseases (e.g. cancer, heart disease) are associated with re-induction of embryonic genes, this information will eventually lead to the better diagnosis and more selective therapeutic treatments. ..
  3. Structural Studies of Galphaq and Its Complexes at the Cell Membrane
    JOHN TESMER; Fiscal Year: 2007
    ..GRK2 and G?q /n are essential for proper heart development and function, RGS2 regulates blood pressure via attenuation of G?q signaling, and p63RhoGEF induces changes in myocytes that are characteristic of cardiac hypertrophy. ..
  4. Calcium channels at ribbon synapses in the retina
    CATHERINE MORGANS; Fiscal Year: 2007
    ..The results of these experiments will provide insight into the composition and functional organization of retinal ribbon synapses and into the perturbation of retinal function associated with CSNB2. ..
  5. Activation of G Protein-Coupled Kinase 2
    JOHN TESMER; Fiscal Year: 2007
    ..abstract_text> ..
  6. The role of nyctalopin in the mammalian retina
    CATHERINE MORGANS; Fiscal Year: 2006
    ..These results will provide insight into the role of nyctalopin in synaptic transmission between photoreceptors and ON-BPCs in the retina, and may lead to the identification of other components of the ON-BPC pathway. ..
  7. EUKARYOTIC GENE REGULATION AS STUDIED IN YEAST
    Mark Ptashne; Fiscal Year: 2004
    ....
  8. Beta/Gamma Subunit Heterogeniety in G Proteins
    JANET ROBISHAW; Fiscal Year: 2004
    ..Thus, studies of these knockout models will likely lead to elucidation of new therapeutic strategies for enhancing the function of the diseased heart. ..
  9. STRUCTURAL BASIS FOR BINDING TO G PROTEIN BETA GAMMA
    Alan Smrcka; Fiscal Year: 2007
    ..4. Identification of sites of interaction G protein betagamma subunits with cellular targets using deuterium exchange mass spectrometry. ..
  10. FASEB SUMMER CONFERENCE ON PHOSPHOLIPASES
    Suzanne Scarlata; Fiscal Year: 2007
    ..The funds requested in this proposal will make an invaluable contribution to the support of this conference and in particular the development of young investigators. ..
  11. ACTIVATION OF PHOSPHOLIPASE C BETA BY G PROTEINS
    SUZANNE F SCARLATA; Fiscal Year: 2010
    ..This proposal seeks to understand on the molecular level howthis activation occurs with the belief that understanding this system on a basic level will allow for the design of more effective and targeted drugs. ..
  12. Beta/Gamma Subunit Heterogeniety in G Proteins
    JANET ROBISHAW; Fiscal Year: 2009
    ....
  13. Molecular basis for the regulation of G protein-coupled receptor kinases
    John J G Tesmer; Fiscal Year: 2010
    ....
  14. Mechanism for Regulation of Phospholipase C by G Protein
    Alan V Smrcka; Fiscal Year: 2010
    ..Elucidation of the functions and mechanisms of this novel Epac/PLCe pathway could lead to novel therapeutic strategies for heart failure, pulmonary disease and hypertension. ..
  15. Selective Targeting of G Protein beta gamma Subunits with Small Molecules
    Alan V Smrcka; Fiscal Year: 2010
    ..Public Health Relevance: Results of these experiments will help to validate this alternate approach to modification of signaling pathways downstream of GPCRs that could ultimately lead to development of novel therapeutics. ..
  16. Novel Mechanisms Regulating the Heterotrimeric G Protein Complex
    Alan Jones; Fiscal Year: 2009
    ....
  17. Mechanism for Regulation of Phospholipase C by G Protein
    Alan Smrcka; Fiscal Year: 2007
    ..Elucidation of the functions and mechanismsof this novel Epac/PLCe pathway could lead to novel therapeutic strategies for heart failure, pulmonary disease and hypertension. ..
  18. Beta/Gamma Subunit Heterogeniety in G Proteins
    JANET ROBISHAW; Fiscal Year: 2007
    ....
  19. Mechanism for Regulation of Phospholipase C by G Protein
    Alan Smrcka; Fiscal Year: 2007
    ..Elucidation of the functions and mechanisms of this novel Epac/PLCe pathway could lead to novel therapeutic strategies for heart failure, pulmonary disease and hypertension. ..
  20. ACTIVATION OF PHOSPHOLIPASE C BETA BY G PROTEINS
    Suzanne Scarlata; Fiscal Year: 2004
    ..We will test this model by a unique combination of in vitro and in vivo methods. ..
  21. G PROTEIN BETA GAMMA SIGNALING SYSTEMS IN VIVO
    JANET ROBISHAW; Fiscal Year: 2001
    ..Validation of results with all of these approaches will provide strong support for the role of a specific beta-gamma dimer in a particular receptor signaling pathway. ..
  22. PHYSICAL ELUCIDATION OF VIRUS ASSEMBLY
    Suzanne Scarlata; Fiscal Year: 2001
    ..Third, the applicant will examine the role of a region of MA in weakening membrane interactions. The proposed study makes it possible to identify critical interactions required for assembly and to design assembly inhibitors. ..
  23. SIGNAL TRANSDUCTION BY G PROTEIN
    Alan Smrcka; Fiscal Year: 1999
    ..Finally, oligomerization of PLC and the potential involvement of this process in regulating PLC activity will be investigated by FRET, sucrose density gradient centrifugation, and fluorescence polarization anisotropy. ..
  24. ADVANCED SEQUENCE BASED PREDICTION OF PROTEIN FUNCTION
    ANDREW NEUWALD; Fiscal Year: 2000
    ..A comprehensive database of protein domains will be made available to the biomedical community through the National Center for Biotechnology Information. ..
  25. An in vivo screen for biological and chemical regulators of mammalial PDEs
    CHARLES HOFFMAN; Fiscal Year: 2007
    ....
  26. DIVERSITY OF A1-ADRENERGIC SIGNALING PATHWAYS IN HEART
    JANET ROBISHAW; Fiscal Year: 1993
    ..The information expected from the proposed studies will begin to elucidate the molecular basis underlying the diversity of alpha1- adrenergic receptor-mediated effects in cardiac muscle during development...
  27. EUKARYOTIC GENE REGULATION AS STUDIED IN YEAST
    Mark Ptashne; Fiscal Year: 2007
    ..In particular we are testing the idea that this protein, a component of the large complex called the Mediator, is rapidly degraded in cells, and that this instability facilitates activation of transcription ..
  28. Modulation of calcium signaling by presenilin-1
    Peter Koulen; Fiscal Year: 2003
    ..Thus, potential new targets for treating those devastating conditions affecting the aging population may be identified. ..
  29. EUKARYOTIC GENE REGULATION AS STUDIED IN YEAST
    Mark Ptashne; Fiscal Year: 2009
    ..In particular we are testing the idea that this protein, a component of the large complex called the Mediator, is rapidly degraded in cells, and that this instability facilitates activation of transcription ..
  30. Predicting common protein mechanisms by the light of evolution
    ANDREW NEUWALD; Fiscal Year: 2007
    ..And (v) propose molecular mechanisms based on these analyses. ..
  31. Nano-HPLC-ESI Quadrupole Ion Trap Mass Spectrometer
    Alan Smrcka; Fiscal Year: 2005
    ..A cost recovery plan will be implemented that will cover costs in future years. ..
  32. Mechanism for Regulation of Phospholipase C by G Protein
    Alan Smrcka; Fiscal Year: 2005
    ..Given the well known involvement of Ras in mitogenesis and cancer, investigation of this mechanism could yield information about PLC involvement in cancer. ..
  33. Heterotrimeric G Protein Signaling in Arabidopsis
    Alan Jones; Fiscal Year: 2005
    ..abstract_text> ..
  34. ACTIVATION OF PHOSPHOLIPASE C BETA BY G PROTEINS
    Suzanne Scarlata; Fiscal Year: 2007
    ..This proposal seeks to understand on the molecular level how this activation occurs with the belief that understanding this system on a basic level will allow for the design of more effective and targeted drugs. ..