cyclic gmp dependent protein kinases

Summary

Summary: A group of enzymes that are dependent on cyclic GMP and catalyzes the phosphorylation of serine or threonine residues of proteins.

Top Publications

  1. ncbi Lethargus is a Caenorhabditis elegans sleep-like state
    David M Raizen
    Center for Sleep and Respiratory Neurobiology, University of Pennsylvania School of Medicine, 3400 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    Nature 451:569-72. 2008
  2. ncbi Intestinal secretory defects and dwarfism in mice lacking cGMP-dependent protein kinase II
    A Pfeifer
    Institut f ur Pharmakologie und Toxikologie, Technische Universitat Munchen, Biedersteiner Strasse 29, D 80802 M unchen, Germany
    Science 274:2082-6. 1996
  3. ncbi Invited review: cGMP-dependent protein kinase signaling mechanisms in smooth muscle: from the regulation of tone to gene expression
    T M Lincoln
    Department of Pathology, Division of Molecular and Cellular Pathology, University of Alabama at Birmingham, Birmingham, Alabama 35294 0019, USA
    J Appl Physiol (1985) 91:1421-30. 2001
  4. ncbi A role for coccidian cGMP-dependent protein kinase in motility and invasion
    Helen I Wiersma
    Department of Human and Animal Infectious Disease Research, Merck Research Laboratories, Merck and Co Inc, PO Box 2000, Rahway, NJ 07065, USA
    Int J Parasitol 34:369-80. 2004
  5. ncbi A 3'UTR pumilio-binding element directs translational activation in olfactory sensory neurons
    Julia A Kaye
    Cellular and Developmental Biology Program, 1 Shields Drive, University of California, Davis, Davis, CA 95616, USA
    Neuron 61:57-70. 2009
  6. ncbi The biology of cyclic GMP-dependent protein kinases
    Franz Hofmann
    Institut fur Pharmakologie und Toxikologie, Technische Universitat Munchen, Biedersteiner Strasse 29, D 80802 München, Germany
    J Biol Chem 280:1-4. 2005
  7. pmc Toxoplasma gondii cyclic GMP-dependent kinase: chemotherapeutic targeting of an essential parasite protein kinase
    Robert G K Donald
    Department of Human and Animal Infectious Disease Research, Merck Research Laboratories, Merck and Co, Inc, Rahway New Jersey 07065, USA
    Eukaryot Cell 1:317-28. 2002
  8. ncbi Molecular mechanism of cGMP-mediated smooth muscle relaxation
    J A Carvajal
    Department of Obstetrics, Gynecology, and Reproductive Sciences, School of Medicine, University of Maryland Baltimore, Baltimore, Maryland 21201, USA
    J Cell Physiol 184:409-20. 2000
  9. ncbi Rising behind NO: cGMP-dependent protein kinases
    F Hofmann
    Institut fur Pharmakologie und Toxikologie, TU Munchen, Biedersteiner Str 29, D 80802 München, Germany tu muenchen de
    J Cell Sci 113:1671-6. 2000
  10. ncbi Anticoccidial kinase inhibitors: identification of protein kinase targets secondary to cGMP-dependent protein kinase
    Robert G K Donald
    Department of Infectious Diseases, Merck and Co, P O Box 2000, R80Y 260 Rahway, NJ 07065 0900, USA
    Mol Biochem Parasitol 149:86-98. 2006

Detail Information

Publications281 found, 100 shown here

  1. ncbi Lethargus is a Caenorhabditis elegans sleep-like state
    David M Raizen
    Center for Sleep and Respiratory Neurobiology, University of Pennsylvania School of Medicine, 3400 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    Nature 451:569-72. 2008
    ..The association of this C. elegans sleep-like state with developmental changes that occur with larval moults suggests that sleep may have evolved to allow for developmental changes...
  2. ncbi Intestinal secretory defects and dwarfism in mice lacking cGMP-dependent protein kinase II
    A Pfeifer
    Institut f ur Pharmakologie und Toxikologie, Technische Universitat Munchen, Biedersteiner Strasse 29, D 80802 M unchen, Germany
    Science 274:2082-6. 1996
    ..The cGKII-deficient mice also developed dwarfism that was caused by a severe defect in endochondral ossification at the growth plates. These results indicate that cGKII plays a central role in diverse physiological processes...
  3. ncbi Invited review: cGMP-dependent protein kinase signaling mechanisms in smooth muscle: from the regulation of tone to gene expression
    T M Lincoln
    Department of Pathology, Division of Molecular and Cellular Pathology, University of Alabama at Birmingham, Birmingham, Alabama 35294 0019, USA
    J Appl Physiol (1985) 91:1421-30. 2001
    ....
  4. ncbi A role for coccidian cGMP-dependent protein kinase in motility and invasion
    Helen I Wiersma
    Department of Human and Animal Infectious Disease Research, Merck Research Laboratories, Merck and Co Inc, PO Box 2000, Rahway, NJ 07065, USA
    Int J Parasitol 34:369-80. 2004
    ..These data clearly demonstrate that cGMP-dependent protein kinase performs an important role in the host-parasite interaction...
  5. ncbi A 3'UTR pumilio-binding element directs translational activation in olfactory sensory neurons
    Julia A Kaye
    Cellular and Developmental Biology Program, 1 Shields Drive, University of California, Davis, Davis, CA 95616, USA
    Neuron 61:57-70. 2009
    ....
  6. ncbi The biology of cyclic GMP-dependent protein kinases
    Franz Hofmann
    Institut fur Pharmakologie und Toxikologie, Technische Universitat Munchen, Biedersteiner Strasse 29, D 80802 München, Germany
    J Biol Chem 280:1-4. 2005
  7. pmc Toxoplasma gondii cyclic GMP-dependent kinase: chemotherapeutic targeting of an essential parasite protein kinase
    Robert G K Donald
    Department of Human and Animal Infectious Disease Research, Merck Research Laboratories, Merck and Co, Inc, Rahway New Jersey 07065, USA
    Eukaryot Cell 1:317-28. 2002
    ..gondii do not respond to treatment. We conclude that PKG represents the primary molecular target responsible for the antiparasitic efficacy of compound 1...
  8. ncbi Molecular mechanism of cGMP-mediated smooth muscle relaxation
    J A Carvajal
    Department of Obstetrics, Gynecology, and Reproductive Sciences, School of Medicine, University of Maryland Baltimore, Baltimore, Maryland 21201, USA
    J Cell Physiol 184:409-20. 2000
    ..In summary, we present compelling evidence supporting a key role for cGMP as a mediator of smooth muscle relaxation in physiological and pharmacological settings...
  9. ncbi Rising behind NO: cGMP-dependent protein kinases
    F Hofmann
    Institut fur Pharmakologie und Toxikologie, TU Munchen, Biedersteiner Str 29, D 80802 München, Germany tu muenchen de
    J Cell Sci 113:1671-6. 2000
    ..cGKII regulates in vivo intestinal fluid secretion by phosphorylation of the cystic fibrosis transmembrane conductance regulator (CFTR), bone growth and renal renin secretion by phosphorylation of unknown proteins...
  10. ncbi Anticoccidial kinase inhibitors: identification of protein kinase targets secondary to cGMP-dependent protein kinase
    Robert G K Donald
    Department of Infectious Diseases, Merck and Co, P O Box 2000, R80Y 260 Rahway, NJ 07065 0900, USA
    Mol Biochem Parasitol 149:86-98. 2006
    ..These results provide an example of the utility of the Toxoplasma model system for investigating the mechanism of action of novel anticoccidial agents...
  11. ncbi Influence of gene action across different time scales on behavior
    Y Ben-Shahar
    Department of Entomology, Neuroscience Program, University of Illinois at Urbana Champaign, 320 Morrill Hall, 505 South Goodwin Avenue, Urbana, IL 61801, USA
    Science 296:741-4. 2002
    ..Previous research showed that allelic differences in PKG expression result in two Drosophila foraging variants. The same gene can thus exert different types of influence on a behavior...
  12. pmc Intramitochondrial signaling: interactions among mitoKATP, PKCepsilon, ROS, and MPT
    Alexandre D T Costa
    Dept of Biology, Portland State Univ, PO Box 751, Portland, OR 97201 0751, USA
    Am J Physiol Heart Circ Physiol 295:H874-82. 2008
    ..This feedback pathway may be responsible for the lasting protective effect of preconditioning, colloquially known as the memory effect...
  13. pmc Cyclic GMP-dependent protein kinase II is a molecular switch from proliferation to hypertrophic differentiation of chondrocytes
    Hirotaka Chikuda
    Department of Sensory and Motor System Medicine, Faculty of Medicine, University of Tokyo, Bunkyo, Tokyo 113 8655, Japan
    Genes Dev 18:2418-29. 2004
    ....
  14. pmc Natural polymorphism affecting learning and memory in Drosophila
    Frederic Mery
    Department of Biology, University of Fribourg, Chemin du Musee 10, CH 1700 Fribourg, Switzerland
    Proc Natl Acad Sci U S A 104:13051-5. 2007
    ....
  15. ncbi Cyclic GMP-dependent protein kinase signaling pathway inhibits RhoA-induced Ca2+ sensitization of contraction in vascular smooth muscle
    V Sauzeau
    Laboratoire de Physiologie Cellulaire et Moleculaire, INSERM U 533, Faculte des Sciences, 44322 Nantes, France
    J Biol Chem 275:21722-9. 2000
    ....
  16. ncbi Cyclic GMP-dependent protein kinase II plays a critical role in C-type natriuretic peptide-mediated endochondral ossification
    Takashi Miyazawa
    Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Sakyo ku, Kyoto 606 8507, Japan
    Endocrinology 143:3604-10. 2002
    ..This study provides in vivo and in vitro genetic evidence that cGKII plays a critical role in CNP-mediated endochondral ossification...
  17. ncbi cAMP- and cGMP-dependent protein kinase phosphorylation sites of the focal adhesion vasodilator-stimulated phosphoprotein (VASP) in vitro and in intact human platelets
    E Butt
    Medizinische Universitatsklinik, Klinische Biochemie und Pathobiochemie, Federal Republic of Germany
    J Biol Chem 269:14509-17. 1994
    ....
  18. ncbi ATP-sensitive K(+) channel activation by nitric oxide and protein kinase G in rabbit ventricular myocytes
    Jin Han
    Department of Physiology and Biophysics, College of Medicine, Inje University, Busan 614 735, Korea
    Am J Physiol Heart Circ Physiol 283:H1545-54. 2002
    ..With the use of 5 nM okadaic acid (a PP2A inhibitor), PP2A had no effect on the channel activity. These results suggest that the NO-cGMP-PKG pathway contributes to phosphorylation of K(ATP) channels in rabbit ventricular myocytes...
  19. ncbi cGMP-dependent changes in phototaxis: a possible role for the foraging gene in honey bee division of labor
    Y Ben-Shahar
    Department of Entomology, University of Illinois at Urbana Champaign, 320 Morrill Hall, 505 S Goodwin Avenue, Urbana, IL 61801, USA
    J Exp Biol 206:2507-15. 2003
    ..Our findings implicate one neural process associated with honey bee division of labor that can be affected by naturally occurring changes in the expression of AMFOR:..
  20. pmc Molecular basis for changes in behavioral state in ant social behaviors
    Christophe Lucas
    Department of Biology, University of Toronto, Ontario, Canada L5L 1C6
    Proc Natl Acad Sci U S A 106:6351-6. 2009
    ..Finally, pharmacological activation of PKG increases defense and reduces foraging behavior. Thus, PKG signaling plays a critical role in P. pallidula behavioral shifts...
  21. ncbi A novel cyclic GMP-dependent protein kinase is expressed in the ring stage of the Plasmodium falciparum life cycle
    Wensheng Deng
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
    Mol Microbiol 44:1141-51. 2002
    ..Biochemical analysis therefore shows that PfPKG is distinct from mammalian PKGs with respect to both cyclic nucleotide analogue activation and inhibition profiles...
  22. ncbi Interaction of fibroblast growth factor and C-natriuretic peptide signaling in regulation of chondrocyte proliferation and extracellular matrix homeostasis
    Pavel Krejci
    Medical Genetics Institute, Cedars Sinai Medical Center, Los Angeles, CA 90048, USA
    J Cell Sci 118:5089-100. 2005
    ..We conclude that CNP utilizes both direct and indirect ways to counteract the effects of FGF signaling in a chondrocyte environment...
  23. pmc The EGL-4 PKG acts with KIN-29 salt-inducible kinase and protein kinase A to regulate chemoreceptor gene expression and sensory behaviors in Caenorhabditis elegans
    Alexander M van der Linden
    Department of Biology, Brandeis University, Waltham, Massachusetts 02454, USA
    Genetics 180:1475-91. 2008
    ..Our results suggest that integration of complex sensory inputs via multiple signaling pathways allows animals to precisely regulate sensory gene expression, thereby appropriately modulating physiology and behavior...
  24. ncbi Intraflagellar transport particles participate directly in cilium-generated signaling in Chlamydomonas
    Qian Wang
    Department of Cell Biology, University of Texas Southwestern Medical School, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA
    Cell 125:549-62. 2006
    ..Our results lead to a model in which the IFT machinery is required not only for assembling cilia and flagella but also for organizing a signaling pathway within the organelles during cilium-generated signaling...
  25. ncbi Regulator of G-protein signaling-2 mediates vascular smooth muscle relaxation and blood pressure
    K Mary Tang
    Molecular Cardiology Research Institute, New England Medical Center and Department of Medicine, Tufts University School of Medicine, Boston, Massachusetts 02111, USA
    Nat Med 9:1506-12. 2003
    ..Our study shows that RGS-2 is required for normal vascular function and blood pressure and is a new drug development target for hypertension...
  26. pmc Reduced inflammatory hyperalgesia with preservation of acute thermal nociception in mice lacking cGMP-dependent protein kinase I
    Irmgard Tegeder
    Pharmazentrum Frankfurt, Klinikum der Johann Wolfgang Goethe Universitat, 60590 Frankfurt am Main, Germany
    Proc Natl Acad Sci U S A 101:3253-7. 2004
    ..The paucity of substance P in laminae I-III may contribute to the reduction of nociception in PKG-I(-/-) mice and suggests a role of PKG-I in substance P synthesis...
  27. ncbi Inhibition of cyclic guanosine 5'-monophosphate-dependent protein kinase I (PKG-I) in lumbar spinal cord reduces formalin-induced hyperalgesia and PKG upregulation
    Achim Schmidtko
    Pharmazentrum Frankfurt, Klinikum der Johann Wolfgang Goethe Universitat, Theodor Stern Kai 7, Frankfurt am Main, 60590, Germany
    Nitric Oxide 8:89-94. 2003
    ..Our data suggest that PKG-I activation is involved in the synaptic transmission of nociceptive stimuli in the spinal cord and that PKG-I inhibitors might be interesting novel drugs for pain treatment...
  28. pmc cGMP-mediated signaling via cGKIalpha is required for the guidance and connectivity of sensory axons
    Hannes Schmidt
    Developmental Neurobiology Group, Medical Research Council, Max Delbruck Centrum fur Molekulare Medizin, Robert Rossle Strasse 10, D 13092 Berlin, Germany
    J Cell Biol 159:489-98. 2002
    ..Our studies therefore reveal that cGMP signaling is important for axonal growth in vivo and in vitro...
  29. ncbi Activation of cGMP-dependent protein kinase by protein kinase C
    Yali Hou
    Department of Biochemistry, Medical College of Georgia, Augusta, Georgia 30912, USA
    J Biol Chem 278:16706-12. 2003
    ..Taken together, these findings outline a novel signal transduction pathway that links PKC stimulation with cyclic nucleotide-independent activation of PKG...
  30. pmc Gametogenesis in malaria parasites is mediated by the cGMP-dependent protein kinase
    Louisa McRobert
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom
    PLoS Biol 6:e139. 2008
    ..falciparum, and demonstrates the feasibility of selective inhibition of a crucial regulator of the malaria parasite life cycle...
  31. ncbi Nerve growth factor and semaphorin 3A signaling pathways interact in regulating sensory neuronal growth cone motility
    Vassil D Dontchev
    Department of Anatomy and Histology, Medical University Sofia, Sofia, 1431 Bulgaria
    J Neurosci 22:6659-69. 2002
    ..These results are consistent with the idea that growth cone responses to an individual guidance cue depend on coincident signaling by other guidance cues and by other regulatory pathways...
  32. ncbi Phosphorylation of NF-kappaB proteins by cyclic GMP-dependent kinase. A noncanonical pathway to NF-kappaB activation
    Bin He
    Department of Radiation Oncology, New England Medical Center, Boston, MA, USA
    Eur J Biochem 270:2174-85. 2003
    ..These observations may bear on mechanisms of programmed cell death in T-lymphocytes. They may also be relevant to ongoing efforts to induce cancer cell apoptosis through activation of PKG...
  33. ncbi Compartmentalization of cardiac beta-adrenergic inotropy modulation by phosphodiesterase type 5
    Eiki Takimoto
    Division of Cardiology, Department of Medicine, Johns Hopkins Medical Institutions, Baltimore, MD 21205, USA
    Circulation 115:2159-67. 2007
    ..The potential role of differential cGMP and protein kinase G stimulation by these 2 modulators was also studied...
  34. pmc Insulin, cGMP, and TGF-beta signals regulate food intake and quiescence in C. elegans: a model for satiety
    Young jai You
    Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Cell Metab 7:249-57. 2008
    ..The EGL-4 cGMP-dependent protein kinase functions downstream of insulin and TGF-beta in sensory neurons including ASI to control quiescence in response to food intake...
  35. ncbi Natural behavior polymorphism due to a cGMP-dependent protein kinase of Drosophila
    K A Osborne
    Department of Biology, York University, 4700 Keele Street, North York, Toronto, Ontario, Canada, M3J 1P3
    Science 277:834-6. 1997
    ..Thus, PKG levels affected food-search behavior, and natural variation in PKG activity accounted for a behavioral polymorphism...
  36. ncbi Protein kinase G from pathogenic mycobacteria promotes survival within macrophages
    Anne Walburger
    Biozentrum, University of Basel, Klingelbergstr 50 70, CH 4056 Basel, Switzerland
    Science 304:1800-4. 2004
    ....
  37. pmc Regulator of G protein signaling 2 mediates cardiac compensation to pressure overload and antihypertrophic effects of PDE5 inhibition in mice
    Eiki Takimoto
    Division of Cardiology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Clin Invest 119:408-20. 2009
    ..Thus, RGS2 is required for early myocardial compensation to pressure overload and mediates the initial antihypertrophic and cardioprotective effects of PDE5 inhibitors...
  38. ncbi The cyclic GMP-dependent protein kinase EGL-4 regulates olfactory adaptation in C. elegans
    Noelle D L'Etoile
    Howard Hughes Medical Institute, Programs in Developmental Biology, Neuroscience, and Genetics, Department of Anatomy, The University of California, San Francisco, San Francisco, CA 94143, USA
    Neuron 36:1079-89. 2002
    ..A predicted nuclear localization signal within EGL-4 is required for adaptation after longer odor exposure, suggesting that nuclear translocation of EGL-4 triggers late forms of adaptation...
  39. ncbi cGMP-dependent protein kinase I mediates the negative inotropic effect of cGMP in the murine myocardium
    Jörg W Wegener
    Pharmakologisches Institut, Universitat Mainz, Germany
    Circ Res 90:18-20. 2002
    ..These results demonstrate that cGKI mediates the negative inotropic effect of cGMP in the myocardium of juvenile and adult mice...
  40. ncbi Heat shock protein 60 or 70 activates nitric-oxide synthase (NOS) I- and inhibits NOS II-associated signaling and depresses the mitochondrial apoptotic cascade during brain stem death
    Julie Y H Chan
    Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung 81346, Taiwan
    J Biol Chem 282:4585-600. 2007
    ..In addition, HSP60 exerts its effects against apoptosis by blunting Mev-induced activation of the Bax/cytochrome c/caspase-3 cascade...
  41. pmc Role of protein kinase G in growth and glutamine metabolism of Mycobacterium bovis BCG
    Liem Nguyen
    Biozentrum, University of Basel, Klingelbergstrasse 50, CH 4056 Basel, Switzerland
    J Bacteriol 187:5852-6. 2005
    ..In vitro growth of Mycobacterium bovis BCG lacking pknG was identical to that of the wild type. We conclude that in M. bovis BCG, glutamine metabolism is not regulated by protein kinase G...
  42. ncbi Dual regulation of the ATP-sensitive potassium channel by activation of cGMP-dependent protein kinase
    Yongping Chai
    Department of Physiology and Membrane Biology, University of California, Rm 4144, Tupper Hall, One Shields Avenue, Davis, CA, 95616, USA
    Pflugers Arch 456:897-915. 2008
    ....
  43. ncbi Rescue of cGMP kinase I knockout mice by smooth muscle specific expression of either isozyme
    Silke Weber
    Institut für Pharmakologie und Toxikologie der Technischen Universität, Biedersteiner Str 29, D 80802 München, Germany
    Circ Res 101:1096-103. 2007
    ..These results show that cGKI in smooth muscle is essential and that either cGKI isozyme alone can rescue basic vascular and intestinal smooth muscle functions...
  44. ncbi Roles of protein kinase A and protein kinase G in synaptic plasticity in the visual cortex
    Shaolin Liu
    Department of Ophthalmology, Yale University School of Medicine, New Haven, CT 06520 8061, USA
    Cereb Cortex 13:864-9. 2003
    ....
  45. ncbi Distribution of cGMP-dependent protein kinase type I and its isoforms in the mouse brain and retina
    S Feil
    Institut fur Pharmakologie und Toxikologie der Technischen Universitat Munchen, Biedersteiner Strasse 29, 80802 Munchen, Germany
    Neuroscience 135:863-8. 2005
    ....
  46. ncbi cGMP-dependent protein kinase II modulates mPer1 and mPer2 gene induction and influences phase shifts of the circadian clock
    Henrik Oster
    Department of Medicine, Division of Biochemistry, University of Fribourg, 1700 Fribourg, Switzerland
    Curr Biol 13:725-33. 2003
    ..The major stimulus for adjustment (resetting) of the clock is nocturnal light. It evokes activation of signaling pathways in the SCN that ultimately lead to expression of mPer1 and mPer2 genes conveying adjustment of the clock...
  47. ncbi The foraging gene of Drosophila melanogaster: spatial-expression analysis and sucrose responsiveness
    A T Belay
    Department of Biology, University of Toronto, Mississauga, Ontario L5L 1C6, Canada
    J Comp Neurol 504:570-82. 2007
    ..We found that pan-neuronal expression of for caused an increase in the SR of sitters, demonstrating a neural function for PKG in this food-related behavior...
  48. ncbi Purification and molecular characterization of cGMP-dependent protein kinase from Apicomplexan parasites. A novel chemotherapeutic target
    Anne M Gurnett
    Department of Human and Animal Infectious Disease Research, Merck Research Laboratories, Rahway, New Jersey 07065, USA
    J Biol Chem 277:15913-22. 2002
    ..Compound 1 is a potent inhibitor of both soluble and membrane-associated isoforms of native PKG, as well as recombinant enzyme, with an IC(50) of <1 nm...
  49. ncbi Circadian clock-controlled regulation of cGMP-protein kinase G in the nocturnal domain
    Shelley A Tischkau
    Department of Cell and Structural Biology, University of Illinois at Urbana Champaign, Urbana, Illinois 60801, USA
    J Neurosci 23:7543-50. 2003
    ..Because sensitivity to phase advance by light-GLU-activated GC-cGMP-PKG occurs in juxtaposition, these signals may induce a premature shift to this PKG-necessary clock state...
  50. pmc Transcriptional up-regulation of nitric oxide synthase II by nuclear factor-kappaB at rostral ventrolateral medulla in a rat mevinphos intoxication model of brain stem death
    Julie Y H Chan
    Department of Medical Education and Research, Kaohsiung Veterans General Hospital, and Center for Neuroscience, National Sun Yat Sen University, Kaohsiung 80424, Taiwan, Republic of China
    J Physiol 581:1293-307. 2007
    ....
  51. ncbi Protein kinase G I immunoreaction is colocalized with arginine-vasopressin immunoreaction in the rat suprachiasmatic nucleus
    Marc Revermann
    Dr Senckenbergische Anatomie, Institut für Anatomie II, Fachbereich Medizin, Johann Wolfgang Goethe Universitat, Theodor Stern Kai 7, D 60590 Frankfurt Main, Germany
    Neurosci Lett 334:119-22. 2002
    ..Within the SCN, PKG-I IR was colocalized with arginine-vasopressin-IR. The intensities of the PKG-I-IR did not vary between day and night...
  52. pmc Defective smooth muscle regulation in cGMP kinase I-deficient mice
    A Pfeifer
    Institut fur Pharmakologie und Toxikologie, Munchen, Biedersteiner, Str 29, 80802 Müchen, Germany
    EMBO J 17:3045-51. 1998
    ..However, cGKI-deficient smooth muscle responded normally to cAMP, indicating that cAMP and cGMP signal via independent pathways, with cGKI being the specific mediator of the NO/cGMP effects in murine smooth muscle...
  53. pmc Natural variation in plasticity of glucose homeostasis and food intake
    Karla R Kaun
    Department of Biology, University of Toronto, 3359 Mississauga Road, Mississauga, Ontario, Canada L5L 1C6
    J Exp Biol 211:3160-6. 2008
    ..Finally, rovers have lower adipokinetic hormone (akh) mRNA levels than sitters. Our data suggest that for mediates larval responses to short-term food deprivation by altering food intake and blood glucose levels...
  54. ncbi Phasic cardiovascular responses to mevinphos are mediated through differential activation of cGMP/PKG cascade and peroxynitrite via nitric oxide generated in the rat rostral ventrolateral medulla by NOS I and II isoforms
    J Y H Chan
    Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung 813, Taiwan, Republic of China
    Neuropharmacology 48:161-72. 2005
    ..We conclude that sGC/cGMP/PKG cascade and peroxynitrite formation may participate in Mev-induced phasic cardiovascular responses as signals downstream to NO generated respectively by NOS I and II in the RVLM...
  55. ncbi Regulation of myosin phosphatase by a specific interaction with cGMP- dependent protein kinase Ialpha
    H K Surks
    Molecular Cardiology Research Institute and Cardiology Division, Department of Medicine, Tufts University School of Medicine and New England Medical Center, Boston, MA 02111, USA
    Science 286:1583-7. 1999
    ..Uncoupling of the cGKIalpha-MBS interaction prevents cGMP-dependent dephosphorylation of myosin light chain, demonstrating that this interaction is essential to the regulation of vascular smooth muscle cell tone...
  56. pmc Nitric oxide-cGMP-protein kinase G pathway negatively regulates vascular transient receptor potential channel TRPC6
    Shinichi Takahashi
    Department of Physiology, Graduate School of Medical Sciences, Fukuoka University, Fukuoka 814 0180, Japan
    J Physiol 586:4209-23. 2008
    ..This mechanism may be physiologically important in vascular tissues where NO is constantly released from vascular endothelial cells or nitrergic nerves...
  57. pmc Activity of cGMP-dependent protein kinase (PKG) affects sucrose responsiveness and habituation in Drosophila melanogaster
    Ricarda Scheiner
    Institut für Ökologie, Technische Universitat Berlin, Franklinstr 28 29, D 10587 Berlin, Germany
    Learn Mem 11:303-11. 2004
    ..These results show that the PKG encoded by for independently affects sensory responsiveness and habituation in Drosophila melanogaster...
  58. ncbi Nitric oxide activates glibenclamide-sensitive K+ channels in urinary bladder myocytes through a c-GMP-dependent mechanism
    Dilip K Deka
    University Department of Pharmacology, Mansfield Road, Oxford, OX1 3QT, UK
    Eur J Pharmacol 492:13-9. 2004
    ..These results suggest that the NO-c-GMP-PKG pathway contributes to activation of K(ATP) channels in guinea-pig urinary bladder myocytes...
  59. ncbi Sensory axon response to substrate-bound Slit2 is modulated by laminin and cyclic GMP
    K T Nguyen-Ba-Charvet
    , INSERM U106, , Paris, 75013, France
    Mol Cell Neurosci 17:1048-58. 2001
    ..These results strongly support the idea that extracellular matrix proteins modulate the response of growth cones to chemotropic molecules by modulating cyclic nucleotide levels...
  60. ncbi Presynaptic and postsynaptic roles of NO, cGK, and RhoA in long-lasting potentiation and aggregation of synaptic proteins
    Hong Gang Wang
    Center for Neurobiology and Behavior, Columbia University, New York, NY 10032, USA
    Neuron 45:389-403. 2005
    ..cGK phosphorylates synaptic VASP during the potentiation, whereas Rho GTPases act both in parallel and upstream of cGMP, in part by maintaining the synaptic localization of soluble guanylyl cyclase...
  61. ncbi Regulation of body size and behavioral state of C. elegans by sensory perception and the EGL-4 cGMP-dependent protein kinase
    Manabi Fujiwara
    Ernest Gallo Clinic and Research Center and Department of Neurology, Programs in Neuroscience and Biomedical Science, University of California, San Francisco, 5858 Horton Street, Suite 200, Emeryville, CA 94608, USA
    Neuron 36:1091-102. 2002
    ..elegans to regulate multiple developmental and behavioral processes including the orchestrated growth of the animal and the expression of particular behavioral states...
  62. ncbi Cyclic GMP-dependent protein kinase Ialpha inhibits thrombin receptor-mediated calcium mobilization in vascular smooth muscle cells
    Eva N Christensen
    Molecular Cardiology Research Institute, Tufts New England Medical Center and Department of Biochemistry, Tufts University School of Medicine, Boston, Massachusetts 02111, USA
    J Biol Chem 281:8409-16. 2006
    ..These results support that PKGIalpha is the principal cGMP-dependent protein kinase isoform mediating inhibition of VSMC activation by the nitric oxide/cyclic GMP pathway...
  63. pmc Cyclic GMP and protein kinase-G in myocardial ischaemia-reperfusion: opportunities and obstacles for survival signaling
    D S Burley
    Division of Pharmacology, Welsh School of Pharmacy, Cardiff University, Cardiff, UK
    Br J Pharmacol 152:855-69. 2007
    ..Overall, the body of experimental evidence suggests that cGMP/PKG survival signalling ameliorates irreversible injury associated with ischaemia-reperfusion and may be a tractable therapeutic target...
  64. pmc Compartmentation and compartment-specific regulation of PDE5 by protein kinase G allows selective cGMP-mediated regulation of platelet functions
    Lindsay S Wilson
    Department of Pathology and Molecular Medicine, Queen s University, Kingston, ON, Canada K7L 3N6
    Proc Natl Acad Sci U S A 105:13650-5. 2008
    ..We submit that our findings may define a molecular mechanism by which PDE5 inhibition can differentially impact selected cellular functions of platelets, and perhaps of other cell types...
  65. ncbi Molecular determinants of the interaction between the inositol 1,4,5-trisphosphate receptor-associated cGMP kinase substrate (IRAG) and cGMP kinase Ibeta
    A Ammendola
    Institut fur Pharmakologie und Toxikologie der Technischen Universitat Munchen, Biedersteiner Strasse 29, D 80802 München, Germany
    J Biol Chem 276:24153-9. 2001
    ..These results show that cGMP induced reduction of cytosolic calcium concentrations requires cGKIbeta and phosphorylation of Ser(696) of IRAG...
  66. ncbi Rhythmicity of the cGMP-related signal transduction pathway in the mammalian circadian system
    G A Ferreyra
    , Universidad Nacional de Quilmes, 1876 Buenos Aires, Argentina
    Am J Physiol Regul Integr Comp Physiol 280:R1348-55. 2001
    ..These results suggest that cGMP and PKG are related to SCN responses to light and undergo diurnal and circadian changes...
  67. ncbi A nonclassical estrogen membrane receptor triggers rapid differential actions in the endocrine pancreas
    Ana B Ropero
    Institute of Bioengineering, Miguel Hernandez University, Campus of San Juan, Alicante 03550, Spain
    Mol Endocrinol 16:497-505. 2002
    ..Therefore, rapid estrogen actions in islet cells are initiated by a nonclassical estrogen membrane receptor...
  68. ncbi Modulation of the NO/CO-cGMP signaling cascade during chronic morphine exposure in mice
    De Yong Liang
    Department of Anesthesiology, Stanford University and Veterans Affairs Palo Alto Health Care System, 112A, 3801 Miranda Avenue, Palo Alto, CA 94304, USA
    Neurosci Lett 365:73-7. 2004
    ....
  69. pmc Low doses of bisphenol A and diethylstilbestrol impair Ca2+ signals in pancreatic alpha-cells through a nonclassical membrane estrogen receptor within intact islets of Langerhans
    Paloma Alonso-Magdalena
    Institute of Bioengineering, Miguel Hernandez University, Sant Joan d Alacant, Alicante, Spain
    Environ Health Perspect 113:969-77. 2005
    ..The action of E2, BPA, and DES in pancreatic alpha-cells may explain some of the effects elicited by endocrine disruptors in the metabolism of glucose and lipid...
  70. pmc Structural basis for the specific inhibition of protein kinase G, a virulence factor of Mycobacterium tuberculosis
    Nicole Scherr
    Biozentrum, University of Basel, CH 4056 Basel, Switzerland
    Proc Natl Acad Sci U S A 104:12151-6. 2007
    ..Our results explain the specific mode of action of AX20017 and demonstrate that virulence factors highly homologous to host molecules can be successfully targeted to block the proliferation of M. tuberculosis...
  71. ncbi Function of cGMP-dependent protein kinases in the nervous system
    Robert Feil
    Institut fur Pharmakologie und Toxikologie, Technische Universitat Munchen, Munchen, Germany
    Rev Neurosci 16:23-41. 2005
    ..Thus, cGKs serve key functions in the transduction of cGMP signals into cellular responses in distinct regions of the nervous system.-..
  72. ncbi Long-term potentiation in hippocampus involves sequential activation of soluble guanylate cyclase, cGMP-dependent protein kinase, and cGMP-degrading phosphodiesterase
    Pilar Monfort
    Instituto de Investigaciones Citologicas, Fundacion Valenciana de Investigaciones Biomedicas, 46010, Valencia, Spain
    J Neurosci 22:10116-22. 2002
    ....
  73. ncbi Protein kinase G transmits the cardioprotective signal from cytosol to mitochondria
    Alexandre D T Costa
    Department of Biology, Portland State University, Portland, Ore, USA
    Circ Res 97:329-36. 2005
    ..We conclude PKG is the terminal cytosolic component of the trigger pathway; it transmits the cardioprotective signal from cytosol to inner mitochondrial membrane by a pathway that includes PKC-epsilon...
  74. ncbi cGMP-dependent protein kinases in drug discovery
    Jens Schlossmann
    Institut fur Pharmakologie und Toxikologie der Technischen Universitat Munchen, Biedersteiner Strasse 29, 80802 Munchen, Germany
    Drug Discov Today 10:627-34. 2005
    ..Isozyme-specific inhibitors and activators of cGK and its downstream substrates might act more specifically than upstream signalling activators, such as organic nitrates and phosphodiesterase inhibitors...
  75. ncbi Cyclic GMP-dependent protein kinases: understanding in vivo functions by gene targeting
    P Ruth
    Institut für Pharmakologie und Toxikologie der Technischen Universität, Munchen, Germany
    Pharmacol Ther 82:355-72. 1999
    ..Mice deficient in cGMP kinases exhibit severe defects and, therefore, may serve as animal models for several human diseases, including hypertension, thrombosis, gastrointestinal dysmotility, and dwarfism...
  76. ncbi Task-specific expression of the foraging gene in harvester ants
    Krista K Ingram
    Department of Biological Sciences, 371 Serra Mall, Stanford University, Stanford, CA 94305, USA
    Mol Ecol 14:813-8. 2005
    ..The association between foraging behaviour and the foraging gene is conserved across social insects but ants and bees have an inverse relationship between foraging expression and behaviour...
  77. ncbi Cyclic GMP-dependent protein kinase EGL-4 controls body size and lifespan in C elegans
    Takashi Hirose
    Department of Biology, Faculty of Sciences, Kyushu University Graduate School, Hakozaki, Fukuoka 812 8581, Japan
    Development 130:1089-99. 2003
    ..Experiments on genetic interaction suggest that the cGMP-EGL-4 signaling pathway represses body size and lifespan through DBL-1/TGF-beta and insulin pathways, respectively...
  78. pmc A novel gain-of-function mutant of the cyclic GMP-dependent protein kinase egl-4 affects multiple physiological processes in Caenorhabditis elegans
    David M Raizen
    Department of Neurology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Genetics 173:177-87. 2006
    ..In a genetic screen we have identified extragenic suppressors of this gain-of-function mutant. Thus, this mutant promises to be a useful tool for identifying downstream targets of PKG...
  79. ncbi Signaling through NO and cGMP-dependent protein kinases
    Jens Schlossmann
    Institut fur Pharmakologie und Toxikologie, Technische Universitat Munchen, Germany
    Ann Med 35:21-7. 2003
    ..Activation of the NO/cGMP/cGKI pathway induces relaxation of smooth muscle by lowering the cytosolic calcium level and/or by calcium desensitization of the contractile elements...
  80. ncbi The mechanism by which the mitochondrial ATP-sensitive K+ channel opening and H2O2 inhibit the mitochondrial permeability transition
    Alexandre D T Costa
    Department of Biology, Portland State University, Portland, Oregon 97201 0751, USA
    J Biol Chem 281:20801-8. 2006
    ..4 (+/-0.1) microm. On the basis of these results, we propose that two different PKCepsilon pools regulate this signaling pathway, one in association with mitoK(ATP) and the other in association with MPT...
  81. ncbi Individual cerebellar Purkinje cells express different cGMP phosphodiesterases (PDEs): in vivo phosphorylation of cGMP-specific PDE (PDE5) as an indicator of cGMP-dependent protein kinase (PKG) activation
    Masami Shimizu-Albergine
    Department of Pharmacology, University of Washington, Seattle, Washington 98195 7280, USA
    J Neurosci 23:6452-9. 2003
    ....
  82. ncbi Vasodilator-stimulated phosphoprotein activation of serum-response element-dependent transcription occurs downstream of RhoA and is inhibited by cGMP-dependent protein kinase phosphorylation
    Shunhui Zhuang
    Department of Medicine and Cancer Center, University of California, San Diego, La Jolla 92093 0652, USA
    J Biol Chem 279:10397-407. 2004
    ..These studies show that VASP, an important component of the cellular microfilament system, plays a major role in regulating SRE-dependent transcription, and that G-kinase regulates VASP activity...
  83. ncbi Cyclic GMP-dependent protein kinases and the cardiovascular system: insights from genetically modified mice
    Robert Feil
    Institut fur Pharmakologie und Toxikologie, Technische Universitat, Biedersteiner Str 29, D 80802 München, Germany
    Circ Res 93:907-16. 2003
    ..Thus, cGKs may represent novel drug targets for the treatment of human cardiovascular disorders...
  84. ncbi The alpha, but not the beta, isoform of the human thromboxane A2 receptor is a target for nitric oxide-mediated desensitization. Independent modulation of Tp alpha signaling by nitric oxide and prostacyclin
    Helen M Reid
    Department of Biochemistry, Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland
    J Biol Chem 278:51190-202. 2003
    ..On the other hand, signaling by TP beta is unaffected by either NO or prostacyclin...
  85. ncbi Anti-apoptotic effect of cGMP in cultured astrocytes: inhibition by cGMP-dependent protein kinase of mitochondrial permeable transition pore
    K Takuma
    Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences and High Technology Research Center, Kobe Gakuin University, Kobe 651 2180 Japan
    J Biol Chem 276:48093-9. 2001
    ..These findings demonstrate that cGMP inhibits the mitochondrial PTP via the activation of PKG, and the prevention of mitochondrial dysfunction contributes to its anti-apoptotic effect...
  86. ncbi Natural variation in food acquisition mediated via a Drosophila cGMP-dependent protein kinase
    Karla R Kaun
    Department of Biology, University of Toronto, 3359 Mississauga Road, Mississauga, Ontario, L5L 1C6, Canada
    J Exp Biol 210:3547-58. 2007
    ..Together, these results show that natural variation in for has far reaching implications affecting a suite of phenotypes involved in the regulation of food acquisition...
  87. ncbi Cysteine-rich protein 2, a novel substrate for cGMP kinase I in enteric neurons and intestinal smooth muscle
    A Huber
    II Medizinische Klinik und Poliklinik, Technische Universitat Munchen, D 81675 Munchen, Germany
    J Biol Chem 275:5504-11. 2000
    ..The co-localization together with the specific phosphorylation of CRP2 by cGKI in vitro and in vivo suggests that CRP2 is a novel substrate of cGKI in neurons and smooth muscle of the small intestine...
  88. ncbi Neuronal polymorphism among natural alleles of a cGMP-dependent kinase gene, foraging, in Drosophila
    J J Renger
    Department of Biological Sciences, University of Iowa, Iowa City, IA 52242, USA
    J Neurosci 19:RC28. 1999
    ..These findings establish the consequences in cellular function for natural variation in an isoform of PKG and suggest a role for natural selection in maintaining variation in neuronal properties...
  89. pmc Natural variation in the thermotolerance of neural function and behavior due to a cGMP-dependent protein kinase
    Ken Dawson-Scully
    University of Toronto, Department of Biology, Mississauga, Ontario, Canada
    PLoS ONE 2:e773. 2007
    ..PKG's role in thermotolerance may also apply to these and other species. Natural variation in thermotolerance arising from genes involved in the PKG pathway could impact the evolution of thermotolerance in natural populations...
  90. ncbi cGMP produced by NO-sensitive guanylyl cyclase essentially contributes to inflammatory and neuropathic pain by using targets different from cGMP-dependent protein kinase I
    Achim Schmidtko
    Pharmazentrum frankfurt ZAFES, Institut fur Klinische Pharmakologie, Klinikum der Johann Wolfgang Goethe Universitat, 60590 Frankfurt am Main, Germany
    J Neurosci 28:8568-76. 2008
    ..Together, our results provide evidence that NO-GC is crucially involved in the central sensitization of pain pathways during inflammatory and neuropathic pain...
  91. ncbi The muscarinic acetylcholine receptor-stimulated increase in aquaporin-5 levels in the apical plasma membrane in rat parotid acinar cells is coupled with activation of nitric oxide/cGMP signal transduction
    Yasuko Ishikawa
    Department of Pharmacology, Tokushima University School of Dentistry, Tokushima, Japan
    Mol Pharmacol 61:1423-34. 2002
    ..These results suggest that NO/cGMP signal transduction has a crucial role in Ca(2+) homeostasis in the mAChR-stimulated increase in AQP5 levels in the APM of rat parotid glands...
  92. ncbi Regulation of RGS2 and second messenger signaling in vascular smooth muscle cells by cGMP-dependent protein kinase
    Patrick Osei-Owusu
    Cell Biology and Physiology and Internal Medicine, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 282:31656-65. 2007
    ..By diminishing cGK activity, endothelial dysfunction may impair RGS2 activation, thereby blunting vascular relaxation and contributing to hypertension...
  93. ncbi NO activation of fos promoter elements requires nuclear translocation of G-kinase I and CREB phosphorylation but is independent of MAP kinase activation
    T Gudi
    Department of Medicine and Cancer Center, University of California, San Diego, La Jolla, California, CA 92093 0652, USA
    Oncogene 19:6324-33. 2000
    ....
  94. pmc Increased adhesion and aggregation of platelets lacking cyclic guanosine 3',5'-monophosphate kinase I
    S Massberg
    Institut für Chirurgische Forschung der Ludwig Maximilians Universität München, 81377 Munchen, Germany
    J Exp Med 189:1255-64. 1999
    ..The defect in platelet cGKI is not compensated by the cAMP/cAMP kinase pathway supporting the essential role of cGKI in prevention of ischemia-induced platelet adhesion and aggregation...
  95. pmc Molecular identification of human G-substrate, a possible downstream component of the cGMP-dependent protein kinase cascade in cerebellar Purkinje cells
    S Endo
    Laboratory for Learning and Memory, RIKEN, Brain Science Institute, Hirosawa 2 1, Wako, 351 0198, Japan
    Proc Natl Acad Sci U S A 96:2467-72. 1999
    ..However, purified G-substrate phosphorylated by cGMP-dependent protein kinase inhibited protein phosphatase 2A more effectively than protein phosphatase 1, suggesting a distinct role as a protein phosphatase inhibitor...
  96. pmc Nitric oxide-induced biphasic mechanism of vascular relaxation via dephosphorylation of CPI-17 and MYPT1
    Toshio Kitazawa
    Boston Biomedical Research Institute, 64 Grove Street, Watertown, MA 02472, USA
    J Physiol 587:3587-603. 2009
    ..Together, these results suggest that NO production suppresses Ca(2+) release, which causes an inactivation of PKC and rapid CPI-17 dephosphorylation as well as MLCK inactivation, resulting in rapid MLC dephosphorylation and relaxation...
  97. ncbi Regulation of glutamate metabolism by protein kinases in mycobacteria
    Helen M O'Hare
    Institute of Chemical Sciences and Engineering, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne, Switzerland
    Mol Microbiol 70:1408-23. 2008
    ..Previous reports of a defect in glutamate metabolism caused by pknG deletion may thus be explained by the effect of unphosphorylated GarA on these two enzyme activities, which may also contribute to the attenuation of virulence...
  98. pmc Differential expression of a virulence factor in pathogenic and non-pathogenic mycobacteria
    Edith N G Houben
    Biozentrum, University of Basel, Klingelbergstrasse 50 70, 4056 Basel, Switzerland
    Mol Microbiol 72:41-52. 2009
    ....
  99. ncbi Exploring the mechanisms of vascular smooth muscle tone with highly specific, membrane-permeable inhibitors of cyclic GMP-dependent protein kinase Ialpha
    Wolfgang R G Dostmann
    Department of Pharmacology, University of Vermont, College of Medicine, Given Building, 89 Beaumont Avenue, Burlington, VT 05405, USA
    Pharmacol Ther 93:203-15. 2002
    ..Collectively, these results indicate that DT-2 and DT-3 effectively inhibit nitric oxide-induced vasodilation, further emphasizing the central role for cGPK in the modulation of vascular contractility...
  100. ncbi Episodic swimming behavior in the nematode C. elegans
    Rajarshi Ghosh
    Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Exp Biol 211:3703-11. 2008
    ..The results show that the motor circuits have a tendency to switch spontaneously between active and inactive behavioral states. This property might be important to the animal in a uniform environment where sensory input is invariant...
  101. ncbi Phosphorylation-dependent inhibition of protein phosphatase-1 by G-substrate. A Purkinje cell substrate of the cyclic GMP-dependent protein kinase
    K U Hall
    Mental Health Research Institute, University of Michigan, Ann Arbor, Michigan 48109, USA
    J Biol Chem 274:3485-95. 1999
    ..These results provide the first demonstration that G-substrate inhibits protein phosphatase-1 and suggest a novel mechanism by which cGMP-dependent protein kinase I can regulate the activity of the type 1 protein phosphatases...

Research Grants63

  1. Nitric Oxide in Hemodialysis AV Fistula Maturation
    Richard A Cohen; Fiscal Year: 2010
    ....
  2. Novel Regenerative Therapy for Myocardial Infarction
    Andrew R Mendelsohn; Fiscal Year: 2011
    ..PUBLIC HEALTH RELEVANCE: We are developing a new regenerative therapy for the treatment of myocardial infarction (heart attacks). A new drug will stimulate the heart to repair itself. ..
  3. ATRIAL NATRIURETIC PEPTIDE AND RECEPTOR GENES IN HYPOXIA
    Yiu Fai Chen; Fiscal Year: 2010
    ..Our proposed mechanistic studies will lead to innovative and effective therapeutic approaches to this important cause of morbidity and mortality in the American population. ..
  4. Peptide Therapy for Pulmonary Arterial Hypertension
    Jawaharlal M Patel; Fiscal Year: 2013
    ..Confirmation of the mechanism-based physiological approach for NO releasing PDE5 inhibitor function of this novel peptide in preclinical animal model is innovative for progression towards Phase I clinical trial for treatment of PH. ..
  5. Redox Regulation of Vascular cGMP Signaling in Neonatal Lungs
    Kathryn N Farrow; Fiscal Year: 2013
    ..They represent the most immediate therapeutic option for these infants if a rationale for their use can be demonstrated. ..
  6. Golgi-associated NO-cGMP Signaling Defect in Muscular Dystrophy
    Gail D Thomas; Fiscal Year: 2013
    ..In general, the knowledge generated by this project will be relevant to many types of muscle diseases. ..
  7. Folate Metabolism in Mycobacterium tuberculosis Revisited: A Potential Drug Targe
    Liem Duy Nguyen; Fiscal Year: 2013
    ....
  8. (Pro)renin receptor and renovascular hypertension
    Helmy M Siragy; Fiscal Year: 2013
    ..These studies are expected to identify novel pathophysiologic mechanisms related to PRR and its contribution to renal injury and could lead to development of new therapeutic strategies in treating hypertension-induced renal disease. ..
  9. Nitric Oxide/cGMP Modulation of Corporal Fibrosis Caused by Neuropraxia
    MONICA GABRIELA FERRINI; Fiscal Year: 2012
    ....
  10. Molecular Mechanisms of Portal Hypertension
    Vijay H Shah; Fiscal Year: 2012
    ....
  11. RGS Regulation of Cardiac Signaling and Hypertrophy
    Ulrike Mende; Fiscal Year: 2010
    ..e., preserving desirable features while targeting those that are maladaptive) through adjusting the activity and/or amount of RGS2. ..
  12. REGULATION OF SEROTONIN TRANSPORTERS
    Randy D Blakely; Fiscal Year: 2013
    ....
  13. Phosphodiesterase 5: A Novel Target and Inhibitor for Breast Cancer Chemopreventi
    Gary A Piazza; Fiscal Year: 2013
    ..The goals of these studies are to validate PDE5 as an anticancer target, determine its potential role in cancer, and identify a safe and effective tadalafil derivative for preclinical drug development. ..
  14. Role of Nitric Oxide and Cyclic GMP in Stem Cells
    David G Gorenstein; Fiscal Year: 2010
    ....
  15. REGULATION OF SEROTONIN TRANSPORTERS
    Randy D Blakely; Fiscal Year: 2010
    ....
  16. REGULATION OF CYCLIC GMP DEPENDENT PROTEIN KINASE
    MICHAEL UHLER; Fiscal Year: 1999
    ..When completed, these studies will bridge a crucial gap in our knowledge concerning the mechanisms by which smooth muscle relaxation is regulated by ANF, nitric oxide, and nitrovasodilators. ..
  17. CGMP DEPENDENT PROTEIN KINASE IN VASCULAR BIOLOGY
    HOWARD SURKS; Fiscal Year: 2003
    ..abstract_text> ..
  18. Magnesium's Antihypertensive Effects in Preeclampsia
    CYNTHIA STANDLEY; Fiscal Year: 2003
    ..Data derived from these experiments will elucidate the anti-hypertensive effects of magnesium, its mode of action and the roles of NO and magnesium in preeclampsia and pregnancy-induced hypertension. ..
  19. CYCLIC GMP REGULATION OF SMOOTH MUSCLE CONTRACTILITY
    Robert Rapoport; Fiscal Year: 1990
    ....
  20. cGMP Phosphodiesterase, a Novel Chemoprevention Target
    Gary Piazza; Fiscal Year: 2005
    ..Finally, cGMP and cAMP levels will be measured in tumors and plasma samples from this study to determine if PDE inhibition occurs in vivo and the utility of cyclic nucleotides as biomarkers. ..
  21. SIGNALING PATHWAY FOR BENZODIAZEPINE INDUCED BEHAVIORS
    RAYMOND QUOCK; Fiscal Year: 1999
    ....
  22. REGULATION OF THE ANP RECEPTOR/GUANYLYL CYCLASE
    Michael Chinkers; Fiscal Year: 2000
    ....
  23. REGULATION OF GENE EXPRESSION BY NO/CGMP
    RENATE PILZ; Fiscal Year: 2002
    ....
  24. PATHOPHYSIOLOGY OF CHRONIC CEREBRAL VASOSPASM
    Robert MacDonald; Fiscal Year: 2005
    ..abstract_text> ..
  25. Bronchial circulation blocks pulmonary oxidant injury
    David Pearse; Fiscal Year: 2006
    ..abstract_text> ..
  26. PRESSURE NATRIURESIS MEDIATED BY EXTRACELLULAR cGMP
    Robert Carey; Fiscal Year: 2009
    ..Proof of the specific aims will identify and characterize a novel role of extracellular Rl cGMP and provide a potential therapeutic target for hypertension and other disease states associated with sodium retention. ..
  27. Molecular Mechanisms of Portal Hypertension
    Vijay Shah; Fiscal Year: 2007
    ..abstract_text> ..
  28. MECHANISMS OF ESTROGEN INDUCED CORONARY VASODILATION
    Richard White; Fiscal Year: 2000
    ..g., osteoporosis, menopausal complications, menstrual problems. ..
  29. Nitric Oxide-Mediated Pulmonary Vasodilation After Chronic Hypoxia
    Thomas Resta; Fiscal Year: 2007
    ..Findings from studies are expected to fundamentally advance our understanding of signal transduction mechanisms by which PKG regulates pulmonary vascular tone, as well as adaptive responses of the pulmonary circulation to CH. ..
  30. CYCLIC GMP SIGNALING PATHWAYS IN RETINA
    SITARAMAYYA ARI; Fiscal Year: 2003
    ..Successful completion of the proposed studies will, for the first time, establish the biochemical mechanism of cyclic GMP action in light adaptation, and identify the proteins that mediate its effects. ..
  31. PKG-dependent inhibition of the MPT
    Peter Pediaditakis; Fiscal Year: 2006
    ..These experiments will provide new information on regulation of the MPT by kinases, for targets of phosphorylation, and relevance of the kinases to MPT-dependent cell death. [unreadable] [unreadable]..
  32. Behavioral Deficits in Ethanol-Exposed nNOS-Null Mice
    Ana Hutton; Fiscal Year: 2005
    ..This project may thereby further support the hypothesis that the NO-cGMP-PKG pathway is protective against alcohol-induced toxicity and the associated motor impairments, which are also observed in FAS children. ..
  33. NO-CGMP INHIBITION OF UROGENITAL SMOOTH MUSCLE
    Christopher Rembold; Fiscal Year: 2004
    ....
  34. Mitochondrial Biogenesis and Neuroprotection
    RITA COWELL; Fiscal Year: 2005
    ..These experiments will address fundamental questions in neurobiology while revealing mechanisms of neuronal survival that could lead to new treatments for patients with neurological disorders. ..
  35. Role of Stretch to Endothelial Pulse-Perfusion Response
    David Kass; Fiscal Year: 2005
    ..abstract_text> ..
  36. Regulation of Oocyte Viability by Granulosa Cell Contact
    John Peluso; Fiscal Year: 2004
    ..abstract_text> ..
  37. Protein Kinase G Regulation of Granulosa Cell Viability
    John Peluso; Fiscal Year: 2006
    ..We now propose to test this hypothesis using biochemical, genetic, imaging and cell biological techniques. [unreadable] [unreadable]..
  38. Cardiovascular Stiffening in Aged Patients with CHF
    David Kass; Fiscal Year: 2005
    ..This could lead to new therapeutic approaches to this difficult clinical problem that affects a growing aged patient population. ..
  39. CATECHOLS AND ADENOSINE IN MYOCARDIAL PRECONDITIONING
    Michael Cohen; Fiscal Year: 2006
    ..Finally, I will see if NECA's salvage of myocardium at reperfusion translates into rapid functional benefits. It is hoped that the potential of this new pharmacologic approach can be defined. ..
  40. Progesterone regulation of human luteal cell viability
    John Peluso; Fiscal Year: 2006
    ..Such selective manipulation of the P4's actions could have far reaching effects on the treatment for infertility, contraception and certain types of cancers. [unreadable] [unreadable]..
  41. CONFOCAL MICROSCOPY SYSTEM
    EARL GODFREY; Fiscal Year: 2001
    ..This instrument would serve as the centerpiece of a modern imaging facility to facilitate NIH funded research at EVMS. ..
  42. Studies of behavioral quiescence in C. elegans
    David Raizen; Fiscal Year: 2008
    ..Raizen while he learns the scientific field of sleep and chronobiology and develops C. elegans as a model system for the study of behavioral quiescence and its relationship to development. ..
  43. CONTROL OF VASCULAR SMOOTH MUSCLE CELL PHENOTYPE BY CGMP
    Thomas Lincoln; Fiscal Year: 2009
    ....
  44. PKC Signaling in cAMP-Induced Pulmonary Vasodilation
    SCOTT BARMAN; Fiscal Year: 2010
    ..Progress of these studies will provide important knowledge towards the development of novel therapeutic agents that will help reduce the morbidity and mortality associated with pulmonary hypertension. ..
  45. Neurovascular Coupling in Hypertension
    Costantino Iadecola; Fiscal Year: 2004
    ..The results of these studies will enhance our understanding of the effects of hypertension on the brain and may provide new insights into treatment strategies aimed at counteracting these detrimental actions. ..
  46. ANTIMITOGENIC MECHANISMS OF PKG IN VASCULAR SM CELLS
    JESSE ROBERTS; Fiscal Year: 2004
    ..In addition, a carefully constructed training program has been developed that will permit successful development of an independent research career examining the basic mechanisms of pulmonary vascular diseases. ..
  47. CYTOKINE DYSFUNCTION AND LUNG 5LO METABOLISM IN AIDS
    Michael Coffey; Fiscal Year: 2002
    ..This proposal will further enhance our ability to correct this defect and help boost AM, PBM, and PMN defenses against opportunistic infections in the lung in HIV disease. ..
  48. REGULATION OF PROSTATE GROWTH BY NEURAL FACTORS
    Kevin McVary; Fiscal Year: 2001
    ..Using this model allows one to explore the limits of understanding prostatic neuroregulation, to explore neurotrophic influences on exocrine gland development, and to possibly uncover novel methods to influence prostate disease. ..
  49. G PROTEIN MEDIATED CA++ SENSITIZATION IN SMOOTH MUSCLE
    Toshio Kitazawa; Fiscal Year: 2002
    ....
  50. Regulation of Angiogenesis via EphB4 Signaling
    JENA STEINLE; Fiscal Year: 2002
    ..These results from both mesenteric and retinal endothelial cells will greatly increase our understanding of Eph B4 receptor regulation of the various stages of angiogenesis. ..
  51. CARDIOVASCULAR RESEARCH TRAINING
    Bradford Berk; Fiscal Year: 2004
    ..The University of Rochester is a research-intensive institution with a long tradition and commitment to training investigators which should facilitate the success of this program in cardiovascular research. ..
  52. Mechanism of apoptosis in lung vascular smooth muscle
    YUICHIRO JUSTIN SUZUKI; Fiscal Year: 2010
    ..These results will be significant because they are expected to provide new agents against pulmonary hypertension. In addition, the results will fundamentally advance the field of lung biology. ..
  53. Generation of Collecting Duct Knockout of PPARgamma
    Tianxin Yang; Fiscal Year: 2006
    ..New information provided by this proposal is expected to provide insights into the roles of PPARgamma in regulation of fluid balance and in mediation of the side effects of TDZ. ..
  54. Fluid Shear Stress and Osteoblast Apoptosis
    FREDRICK PAVALKO; Fiscal Year: 2007
    ..We will use primary cultures of rat calvarial osteoblasts, and osteoblast cell lines, including MC3T3-E1 and UMR106.01 cells, to investigate the cellular mechanisms that regulate apoptosis. [unreadable] [unreadable]..
  55. SYNAPTIC CONNECTIONS OF SPINAL CORD NEURONS
    William Willis; Fiscal Year: 2007
    ..Preliminary evidence supports the feasibility of the work and suggests that the results could lead to a number of new targets for improvements in pain therapy. ..
  56. 2007 Vascular Cell Biology Gordon Research Conference
    Bradford Berk; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  57. Mechanisms of PKG-induced PASMC Differentiation
    JESSE ROBERTS; Fiscal Year: 2008
    ..They are likely to provide information that will lead to the development of novel therapeutic targets that can be used to prevent pulmonary vascular disease in the injured lung. [unreadable] [unreadable] [unreadable] [unreadable]..
  58. EXPERIMENTAL NEUROGENIC HYPERTENSION PROGRAM
    Costantino Iadecola; Fiscal Year: 2008
    ..The proposed studies are relevant to human diseases, such as hypertension-induced cognitive dysfunction, cardiovascular complications of sleep apnea, and cardiovascular diseases in post-menopausal women. ..
  59. Pancreatic Function: G-Protein Mediated Ca2+ Signaling
    David Yule; Fiscal Year: 2007
    ..abstract_text> ..
  60. OPIOID INDUCED REM SLEEP INHIBITION
    Ralph Lydic; Fiscal Year: 2005
    ..The potential health relatedness of these basic studies derives from the unwanted side effects of opioids on sleep and breathing. ..
  61. REACTIVE OXYGEN SPECIES AND VASCULAR O2 SENSING
    Michael S Wolin; Fiscal Year: 2010
    ..These studies should help define the origins of differences in physiological responses of pulmonary and coronary arteries to changes in O2 tension. ..
  62. PULMONARY VASODILATION AND GUANYLATE CYCLASE REGULATION
    Michael S Wolin; Fiscal Year: 2010
    ....