flavin mononucleotide

Summary

Summary: A coenzyme for a number of oxidative enzymes including NADH DEHYDROGENASE. It is the principal form in which RIBOFLAVIN is found in cells and tissues.

Top Publications

  1. ncbi The speed of RNA transcription and metabolite binding kinetics operate an FMN riboswitch
    J Kenneth Wickiser
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
    Mol Cell 18:49-60. 2005
  2. ncbi Flavogenomics--a genomic and structural view of flavin-dependent proteins
    Peter Macheroux
    Institute of Biochemistry, Graz University of Technology, Graz, Austria
    FEBS J 278:2625-34. 2011
  3. pmc A crystallographic study of Cys69Ala flavodoxin II from Azotobacter vinelandii: structural determinants of redox potential
    Sharmini Alagaratnam
    Leiden Institute of Chemistry, P O Box 9502, 2300 RA Leiden, The Netherlands
    Protein Sci 14:2284-95. 2005
  4. pmc Control of electron transfer and catalysis in neuronal nitric-oxide synthase (nNOS) by a hinge connecting its FMN and FAD-NADPH domains
    Mohammad Mahfuzul Haque
    Department of Pathobiology, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA
    J Biol Chem 287:30105-16. 2012
  5. pmc Photochemical properties of the flavin mononucleotide-binding domains of the phototropins from Arabidopsis, rice, and Chlamydomonas reinhardtii
    Masahiro Kasahara
    National Institute for Basic Biology, Okazaki 444 8585, Japan
    Plant Physiol 129:762-73. 2002
  6. pmc Monitoring shifts in the conformation equilibrium of the membrane protein cytochrome P450 reductase (POR) in nanodiscs
    Maria Wadsäter
    Nano Science Center and Institute of Chemistry, Faculty of Science, University of Copenhagen, DK 2200 Copenhagen, Denmark
    J Biol Chem 287:34596-603. 2012
  7. pmc Effect of the synthetic NC-1059 peptide on diffusion of riboflavin across an intact corneal epithelium
    Yuntao Zhang
    Division of Biology, Kansas State University, Manhattan, KS 66506 4901, USA
    Invest Ophthalmol Vis Sci 53:2620-9. 2012
  8. ncbi Biomechanical and histological changes after corneal crosslinking with and without epithelial debridement
    Gregor Wollensak
    Department of Ophthalmology, Eye Laser Institute, Martin Luther University, Halle, Germany
    J Cataract Refract Surg 35:540-6. 2009
  9. pmc Molecular basis of reduced pyridoxine 5'-phosphate oxidase catalytic activity in neonatal epileptic encephalopathy disorder
    Faik N Musayev
    Department of Medicinal Chemistry, School of Pharmacy, and Institute for Structural Biology and Drug Discovery, Virginia Commonwealth University, Richmond, Virginia 23219, USA
    J Biol Chem 284:30949-56. 2009
  10. ncbi Riboflavin, flavin mononucleotide, and flavin adenine dinucleotide in human plasma and erythrocytes at baseline and after low-dose riboflavin supplementation
    Steinar Hustad
    LOCUS for Homocysteine and Related Vitamins, University of Bergen, Armauer Hansens Hus, N 5021 Bergen, Norway
    Clin Chem 48:1571-7. 2002

Research Grants

  1. Flavoenzymes in Pyrimidine Metabolism
    Bruce A Palfey; Fiscal Year: 2011
  2. Nitrite Mediated Cardioprotection
    DAVID JOSEPH LEFER; Fiscal Year: 2012
  3. Pharmacogenomics of Human P450 Oxidoreductase
    Walter L Miller; Fiscal Year: 2012
  4. Real-time detection of cancer biomarkers using a self-sustained aptazyme
    Charles Olea; Fiscal Year: 2013
  5. Non-coding RNA Structure through a Mutate-and-Map Strategy
    Rhiju Das; Fiscal Year: 2013
  6. Structural Basis for Isoprenoid Biosynthesis
    JOSEPH NOEL; Fiscal Year: 2006
  7. Biosynthesis and Physiology of Vitamin B12 in Prokaryotes
    MICHIKO TAGA; Fiscal Year: 2009
  8. Mechanistic Studies of Type II IPP Isomerase
    Steven Rothman; Fiscal Year: 2006
  9. COFACTOR ROLE IN BETA-SHEET PROTEIN FOLDING
    Pernilla Wittung Stafshede; Fiscal Year: 2005
  10. RNA APTAMER COMPLEXES--STRUCTURES AND INTERACTIONS
    Dinshaw Patel; Fiscal Year: 1999

Detail Information

Publications268 found, 100 shown here

  1. ncbi The speed of RNA transcription and metabolite binding kinetics operate an FMN riboswitch
    J Kenneth Wickiser
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
    Mol Cell 18:49-60. 2005
    ..A flavin mononucleotide (FMN)-dependent riboswitch from the ribDEAHT operon of Bacillus subtilis uses a transcription termination ..
  2. ncbi Flavogenomics--a genomic and structural view of flavin-dependent proteins
    Peter Macheroux
    Institute of Biochemistry, Graz University of Technology, Graz, Austria
    FEBS J 278:2625-34. 2011
    ..An understanding of 'flavin-intensive' lifestyles, such as in the human pathogen Mycobacterium tuberculosis, may result in valuable new intervention strategies that target either riboflavin biosynthesis or uptake...
  3. pmc A crystallographic study of Cys69Ala flavodoxin II from Azotobacter vinelandii: structural determinants of redox potential
    Sharmini Alagaratnam
    Leiden Institute of Chemistry, P O Box 9502, 2300 RA Leiden, The Netherlands
    Protein Sci 14:2284-95. 2005
    ..The flavin mononucleotide (FMN) cofactor is flanked by a leucine on its re face instead of the more conserved tryptophan, resulting ..
  4. pmc Control of electron transfer and catalysis in neuronal nitric-oxide synthase (nNOS) by a hinge connecting its FMN and FAD-NADPH domains
    Mohammad Mahfuzul Haque
    Department of Pathobiology, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA
    J Biol Chem 287:30105-16. 2012
    ....
  5. pmc Photochemical properties of the flavin mononucleotide-binding domains of the phototropins from Arabidopsis, rice, and Chlamydomonas reinhardtii
    Masahiro Kasahara
    National Institute for Basic Biology, Okazaki 444 8585, Japan
    Plant Physiol 129:762-73. 2002
    ..or voltage (LOV) domains at their N termini (LOV1 and LOV2), each a binding site for the chromophore flavin mononucleotide (FMN). Their C termini contain a serine/threonine protein kinase domain...
  6. pmc Monitoring shifts in the conformation equilibrium of the membrane protein cytochrome P450 reductase (POR) in nanodiscs
    Maria Wadsäter
    Nano Science Center and Institute of Chemistry, Faculty of Science, University of Copenhagen, DK 2200 Copenhagen, Denmark
    J Biol Chem 287:34596-603. 2012
    ..Upon complete reduction by NADPH, the conformational equilibrium shifts toward the compact form protecting the reduced FMN cofactor from engaging in unspecific electron transfer reaction...
  7. pmc Effect of the synthetic NC-1059 peptide on diffusion of riboflavin across an intact corneal epithelium
    Yuntao Zhang
    Division of Biology, Kansas State University, Manhattan, KS 66506 4901, USA
    Invest Ophthalmol Vis Sci 53:2620-9. 2012
    ..To investigate the effect of the peptide NC-1059 on riboflavin (RF) diffusion across an intact corneal epithelium into the stroma...
  8. ncbi Biomechanical and histological changes after corneal crosslinking with and without epithelial debridement
    Gregor Wollensak
    Department of Ophthalmology, Eye Laser Institute, Martin Luther University, Halle, Germany
    J Cataract Refract Surg 35:540-6. 2009
    ..To test the biomechanical efficiency of corneal crosslinking with riboflavin without epithelial debridement (C3-R)...
  9. pmc Molecular basis of reduced pyridoxine 5'-phosphate oxidase catalytic activity in neonatal epileptic encephalopathy disorder
    Faik N Musayev
    Department of Medicinal Chemistry, School of Pharmacy, and Institute for Structural Biology and Drug Discovery, Virginia Commonwealth University, Richmond, Virginia 23219, USA
    J Biol Chem 284:30949-56. 2009
    ..These results provide a molecular basis for the phenotype associated with the R229W mutation, as well as providing a foundation for understanding the pathophysiological consequences of pyridoxine 5'-phosphate oxidase mutations...
  10. ncbi Riboflavin, flavin mononucleotide, and flavin adenine dinucleotide in human plasma and erythrocytes at baseline and after low-dose riboflavin supplementation
    Steinar Hustad
    LOCUS for Homocysteine and Related Vitamins, University of Bergen, Armauer Hansens Hus, N 5021 Bergen, Norway
    Clin Chem 48:1571-7. 2002
    Vitamin B(2) exists in blood as riboflavin and its cofactors, flavin mononucleotide (FMN) and FAD. The erythrocyte glutathione reductase activation coefficient (EGRAC) has traditionally been used to assess vitamin B(2) status in humans...
  11. pmc Crystal structure of the flavoprotein ArsH from Sinorhizobium meliloti
    Jun Ye
    Department of Biochemistry and Molecular Biology, Wayne State University, School of Medicine, 540 East Canfield Avenue, Detroit, MI 48201, USA
    FEBS Lett 581:3996-4000. 2007
    ..The N- and C-terminal extensions of ArsH are involved in interactions between subunits and tetramer formation. The structure may provide insight in how ArsH participates in arsenic detoxification...
  12. ncbi Antimicrobial efficacy of riboflavin/UVA combination (365 nm) in vitro for bacterial and fungal isolates: a potential new treatment for infectious keratitis
    Suy Anne R Martins
    Wilmer Ophthalmological Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA
    Invest Ophthalmol Vis Sci 49:3402-8. 2008
    ..To demonstrate the antimicrobial properties of riboflavin/UVA (365 nm) against common pathogens...
  13. pmc A novel chromate reductase from Thermus scotoductus SA-01 related to old yellow enzyme
    Diederik Johannes Opperman
    Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, Bloemfontein 9300, South Africa
    J Bacteriol 190:3076-82. 2008
    ..protein, with a monomer molecular mass of approximately 36 kDa, containing a noncovalently bound flavin mononucleotide cofactor. The chromate reductase is optimally active at a pH of 6...
  14. pmc Structural and mechanistic aspects of flavoproteins: electron transfer through the nitric oxide synthase flavoprotein domain
    Dennis J Stuehr
    Department of Pathobiology, Lerner Research Institute, Cleveland, OH 44195, USA
    FEBS J 276:3959-74. 2009
    ....
  15. ncbi New frontiers in structural flavoenzymology
    Luigi De Colibus
    Dipartimento di Genetica e Microbiologia, Universita di Pavia, Via Ferrata 1, 27100 Pavia, Italy
    Curr Opin Struct Biol 16:722-8. 2006
    ..The integration of the enzymology data with crystallographic studies at atomic resolution is resulting in unprecedented insight into the chemical and geometric properties underlying flavoenzyme function...
  16. pmc Linear free energy relationships demonstrate a catalytic role for the flavin mononucleotide coenzyme of the type II isopentenyl diphosphate:dimethylallyl diphosphate isomerase
    Christopher J Thibodeaux
    Division of Medicinal Chemistry, College of Pharmacy, Department of Chemistry and Biochemistry, and Institute of Cellular and Molecular Biology, University of Texas, Austin, Texas 78712, USA
    J Am Chem Soc 132:9994-6. 2010
    ..Surprisingly, IDI-2 requires a reduced flavin mononucleotide (FMN) coenzyme to carry out this redox neutral isomerization...
  17. ncbi Biochemical characterization of WrbA, founding member of a new family of multimeric flavodoxin-like proteins
    R Grandori
    Department of Chemistry, Princeton University, Princeton, New Jersey 08544 1009, USA
    J Biol Chem 273:20960-6. 1998
    ..Although many proteins with flavodoxin-like domains are known to be multimers, WrbA is apparently the first characterized case in which multimerization is associated directly with the flavodoxin-like domain itself...
  18. ncbi Reactions of the flavin mononucleotide in complex I: a combined mechanism describes NADH oxidation coupled to the reduction of APAD+, ferricyanide, or molecular oxygen
    James A Birrell
    Medical Research Council Mitochondrial Biology Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, UK
    Biochemistry 48:12005-13. 2009
    ..Alternatively, NADH oxidation, by the flavin mononucleotide in complex I, can be coupled to the reduction of hydrophilic electron acceptors, in non-energy-transducing ..
  19. pmc BluB cannibalizes flavin to form the lower ligand of vitamin B12
    Michiko E Taga
    Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Nature 446:449-53. 2007
    ..Here we show that BluB triggers the unprecedented fragmentation and contraction of the bound flavin mononucleotide cofactor and cleavage of the ribityl tail to form DMB and D-erythrose 4-phosphate...
  20. pmc Structure determination of an FMN reductase from Pseudomonas aeruginosa PA01 using sulfur anomalous signal
    Rakhi Agarwal
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA
    Acta Crystallogr D Biol Crystallogr 62:383-91. 2006
    ..this protein lacks the flavodoxin key fingerprint motif [(T/S)XTGXT], it has been confirmed to bind flavin mononucleotide and the binding site was identified via X-ray crystallography...
  21. ncbi Dissecting the energetics of the apoflavodoxin-FMN complex
    A Lostao
    Departamento de Bioquimica y Biologia Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, 50009 Zaragoza, Spain
    J Biol Chem 275:9518-26. 2000
    ..Interestingly, we find crystallographic evidence of flexibility in one of the loops (57-62) involved in isoalloxazine binding...
  22. pmc Six new candidate members of the alpha/beta twisted open-sheet family detected by sequence similarity to flavodoxin
    R Grandori
    Chemistry Department, Princeton University, New Jersey 08544, USA
    Protein Sci 3:2185-93. 1994
    ..Our analysis also suggests that the helix-turn-helix motif we identified previously in the C-terminal region of the WrbA family is unlikely to reflect a DNA-binding function of this new protein family...
  23. pmc Flavin mononucleotide-based fluorescent reporter proteins outperform green fluorescent protein-like proteins as quantitative in vivo real-time reporters
    Thomas Drepper
    Institute of Molecular Enzyme Technology, Heinrich Heine University Duesseldorf, Research Center Juelich, Stetternicher Forst, D 52426 Juelich, Germany
    Appl Environ Microbiol 76:5990-4. 2010
    ..Instead, flavin mononucleotide (FMN)-binding fluorescent proteins (FbFPs) are suitable for quantitative real-time in vivo assays under ..
  24. pmc Genetic transformation of an obligate anaerobe, P. gingivalis for FMN-green fluorescent protein expression in studying host-microbe interaction
    Chul Hee Choi
    Department of Periodontology, University of Florida, Gainesville, Florida, United States of America
    PLoS ONE 6:e18499. 2011
    The recent introduction of "oxygen-independent" flavin mononucleotide (FMN)-based fluorescent proteins (FbFPs) is of major interest to both eukaryotic and prokaryotic microbial biologists...
  25. ncbi A second FMN binding site in yeast NADPH-cytochrome P450 reductase suggests a mechanism of electron transfer by diflavin reductases
    David C Lamb
    Wolfson Laboratory of P450 Biodiversity, Swansea Medical School University of Wales Swansea, Swansea, Wales SA2 8PP, UK
    Structure 14:51-61. 2006
    ....
  26. ncbi X-ray structure of Escherichia coli pyridoxine 5'-phosphate oxidase complexed with pyridoxal 5'-phosphate at 2.0 A resolution
    M K Safo
    Institute for Structural Biology and Drug Discovery, and Department of Biochemistry, Virginia Commonwealth University, Richmond, VA 23219, USA
    J Mol Biol 310:817-26. 2001
    ..A possible tunnel exists between the two sites so that pyridoxal 5'-phosphate formed at the active site may transfer to the non-catalytic site without passing though the solvent...
  27. ncbi Redox reactions of reduced flavin mononucleotide (FMN), riboflavin (RBF), and anthraquinone-2,6-disulfonate (AQDS) with ferrihydrite and lepidocrocite
    Zhi Shi
    Pacific Northwest National Laboratory, P O Box 999, MS K8 96, Richland, Washington 99352, USA
    Environ Sci Technol 46:11644-52. 2012
    ..importance of flavins in Fe(III) bioreduction, we investigated the redox reaction kinetics of reduced flavin mononucleotide (i.e., FMNH(2)) and reduced riboflavin (i.e., RBFH(2)) with ferrihydrite and lepidocrocite...
  28. ncbi Comparisons of wild-type and mutant flavodoxins from Anacystis nidulans. Structural determinants of the redox potentials
    D M Hoover
    Biophysics Research Division and Department of Biological Chemistry, University of Michigan, 930 N Univeristy Ave, Ann Arbor, MI 48109, USA
    J Mol Biol 294:725-43. 1999
    ..The potentials and the effects of mutations in A. nidulans flavodoxin are rationalized using a thermodynamic scheme developed for C. beijerinckii flavodoxin...
  29. pmc Crystal structures of the tryptophan repressor binding protein WrbA and complexes with flavin mononucleotide
    Jason Gorman
    Department of Biochemistry and Molecular Biophysics, Columbia University, 630 West 168th Street, Box 18, New York, NY 10032, USA
    Protein Sci 14:3004-12. 2005
    ..structures of WrbA from Deinococcus radiodurans and Pseudomonas aeruginosa and their complexes with flavin mononucleotide. The protomer structure is similar to that of previously determined long-chain flavodoxins; however, each ..
  30. ncbi Quantification of riboflavin, flavin mononucleotide, and flavin adenine dinucleotide in human plasma by capillary electrophoresis and laser-induced fluorescence detection
    S Hustad
    Department of Pharmacology, University of Bergen, Armauer Hansens Hus, 5021 Bergen, Norway
    Clin Chem 45:862-8. 1999
    Riboflavin is the precursor of flavin mononucleotide (FMN) and FAD, which serve as cofactors for several redox enzymes...
  31. ncbi Crystal structures of a novel ferric reductase from the hyperthermophilic archaeon Archaeoglobus fulgidus and its complex with NADP+
    H J Chiu
    Division of Chemistry and Chemical Engineering, California Institute of Technology, 91125, USA
    Structure 9:311-9. 2001
    ..However, the high cellular abundance of the A. fulgidus FeR (approximately 0.75% of the total soluble protein) suggests a catabolic role for this enzyme as the terminal electron acceptor in a ferric iron-based respiratory pathway [1]...
  32. ncbi Effect of the Insertion of a Glycine Residue into the Loop Spanning Residues 536-541 on the Semiquinone State and Redox Properties of the Flavin Mononucleotide-Binding Domain of Flavocytochrome P450BM-3 from Bacillus megaterium
    Huai Chun Chen
    Department of Biochemistry and Ohio State Biochemistry Program, The Ohio State University, Columbus, Ohio 43210
    Biochemistry 47:13788-99. 2008
    ..including the inability to thermodynamically stabilize the neutral semiquinone (SQ) state of the flavin mononucleotide (FMN) cofactor. Rather, the anionic SQ species is only transiently formed during rapid reduction...
  33. pmc Flavin mononucleotide-based fluorescent protein as an oxygen-independent reporter in Candida albicans and Saccharomyces cerevisiae
    D Tielker
    Institut fur Mikrobiologie, Forschungszentrum Julich, Heinrich Heine Universitat Dusseldorf, Dusseldorf, Germany
    Eukaryot Cell 8:913-5. 2009
    Hypoxia is encountered frequently by pathogenic and apathogenic fungi. A codon-adapted gene encoding flavin mononucleotide-based fluorescent protein (CaFbFP) was expressed in Candida albicans and Saccharomyces cerevisiae...
  34. pmc Flavin mononucleotide-based fluorescent proteins function in mammalian cells without oxygen requirement
    Janine Walter
    Department of Neurology and Epileptology, Hertie Institute for Clinical Brain Research, Eberhard Karls University, Tubingen, Baden Wurttemberg, Germany
    PLoS ONE 7:e43921. 2012
    ..The fluorescence of flavin mononucleotide (FMN)-based fluorescent proteins (FbFPs) does not require molecular oxygen...
  35. ncbi Contributions of the 8-methyl group to the vibrational normal modes of flavin mononucleotide and its 5-methyl semiquinone radical
    Azaria S Eisenberg
    Department of Chemistry, New York University, 100 Washington Square East, New York, New York 10003, USA
    J Phys Chem A 112:6179-89. 2008
    ..In this paper, we report the resonance and pre-resonance Raman spectra of flavin mononucleotide (FMN) and its N5-methyl neutral radical semiquinone (5-CH 3FMN(*)), of which the 8-methyl group of ring I ..
  36. ncbi The closed and compact domain organization of the 70-kDa human cytochrome P450 reductase in its oxidized state as revealed by NMR
    Bruno Vincent
    Centre de Recherche de Gif, Institut de Chimie des Substances Naturelles, CNRS, 1 Avenue de la Terrasse, 91190 Gif sur Yvette, France
    J Mol Biol 420:296-309. 2012
    ..structures of mammalian and yeast CPRs show a compact organization for the two domains containing FMN (flavin mononucleotide) and FAD (flavin adenine dinucleotide), with a short interflavin distance consistent with fast ET from the ..
  37. ncbi Flavin adenine dinucleotide and flavin mononucleotide metabolism in rat liver--the occurrence of FAD pyrophosphatase and FMN phosphohydrolase in isolated mitochondria
    M Barile
    Dipartimento di Biochimica e Biologia Molecolare, Universita di Bari, and Centro di Studio sui Mitocondri e Metabolismo Energetico, C N R, Italy
    Eur J Biochem 249:777-85. 1997
    ....
  38. pmc Conformational changes of NADPH-cytochrome P450 oxidoreductase are essential for catalysis and cofactor binding
    Chuanwu Xia
    Department of Biochemistry, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Biol Chem 286:16246-60. 2011
    ....
  39. ncbi Refined structures of oxidized flavodoxin from Anacystis nidulans
    C L Drennan
    Department of Biological Chemistry and Biophysics Research Division, University of Michigan, 930 N University Ave, Ann Arbor, MI 48109, USA
    J Mol Biol 294:711-24. 1999
    ..The structures reported here provide the starting point for structure-function studies of the reduced states and of mutants, described in the accompanying paper...
  40. ncbi Relation between riboflavin, flavin mononucleotide and flavin adenine dinucleotide concentrations in plasma and red cells in patients with critical illness
    Aikaterini T Vasilaki
    University Department of Surgery, University of Glasgow Faculty of Medicine, Royal Infirmary, Glasgow G312ER, Scotland, United Kingdom
    Clin Chim Acta 411:1750-5. 2010
    ..The aim of the present study was to examine the longitudinal interrelationships between riboflavin, flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) in plasma and red cells in patients with critical illness.
  41. ncbi Photo-reduction of flavin mononucleotide to semiquinone form in LOV domain mutants of blue-light receptor phot from Chlamydomonas reinhardtii
    S H Song
    Institut fur Physikalische und Theoretische Chemie, Universitat Regensburg, D 93053 Regensburg, Germany
    J Photochem Photobiol B 87:37-48. 2007
    ..Blue-light exposure reduced the fully oxidized flavin mononucleotide, FMN(ox), to FMN semiquinone, FMNH*, (quantum efficiency around 1%) which further reduced to FMN ..
  42. pmc The riboflavin analog roseoflavin targets an FMN-riboswitch and blocks Listeria monocytogenes growth, but also stimulates virulence gene-expression and infection
    Mikael Mansjö
    Department of Molecular Biology, Umea University, Umea, Sweden
    RNA Biol 8:674-80. 2011
    ..monocytogenes virulence gene expression and infection abilities in a mechanism independent of the FMN riboswitch. Our results suggest that roseoflavin can block growth but also enhance Listeria virulence...
  43. pmc Staphylococcus aureus NfrA (SA0367) is a flavin mononucleotide-dependent NADPH oxidase involved in oxidative stress response
    Karin Streker
    Institut für Molekulare Infektionsbiologie, Rontgenring 11, D 97070 Wurzburg, Germany
    J Bacteriol 187:2249-56. 2005
    ..aureus. The purified protein oxidized NADPH but not NADH, producing NADP in the presence of flavin mononucleotide, suggesting that NfrA is an NADPH oxidase in S. aureus...
  44. ncbi Redox properties of the isolated flavin mononucleotide- and flavin adenine dinucleotide-binding domains of neuronal nitric oxide synthase
    Pierre E Garnaud
    School of Chemistry, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JJ, UK
    Biochemistry 43:11035-44. 2004
    ..Here we report the preparation of the isolated flavin mononucleotide (FMN)-binding domain of nNOS with bound CaM and the electrochemical analysis of this and the isolated ..
  45. ncbi Sensing small molecules by nascent RNA: a mechanism to control transcription in bacteria
    Alexander S Mironov
    State Research Institute of Genetics and Selection of Industrial Microorganisms, 113545, Moscow, Russia
    Cell 111:747-56. 2002
    Thiamin and riboflavin are precursors of essential coenzymes-thiamin pyrophosphate (TPP) and flavin mononucleotide (FMN)/flavin adenine dinucleotide (FAD), respectively...
  46. pmc Crystal structure of ChrR--a quinone reductase with the capacity to reduce chromate
    Subramaniam Eswaramoorthy
    Department of Biology, Brookhaven National Laboratory, Upton, New York, USA
    PLoS ONE 7:e36017. 2012
    ..2 Å resolution, shows that it belongs to the flavodoxin superfamily in which flavin mononucleotide (FMN) is firmly anchored to the protein...
  47. ncbi Spectroscopic characterization of flavin mononucleotide bound to the LOV1 domain of Phot1 from Chlamydomonas reinhardtii
    W Holzer
    Institut II Experimentelle und Angewandte Physik, Universitat Regensburg, Germany
    Photochem Photobiol 75:479-87. 2002
    The absorption and emission behavior of flavin mononucleotide (FMN) in the light-, oxygen- and voltage-sensitive (LOV) domain LOV1 of the photoreceptor Phot1 from the green alga Chlamydomonas reinhardtii was studied...
  48. ncbi Biochemistry: molecular cannibalism
    Steven E Ealick
    Nature 446:387-8. 2007
  49. ncbi Flavin mononucleotide-binding domain of the flavoprotein component of the sulfite reductase from Escherichia coli
    J Coves
    Laboratoire d Etudes Dynamiques et Structurales de la Sélectivité, Unité Mixte de Recherche du Centre National de la Recherche Scientifique 5616, Universite Joseph Fourier, Grenoble, France
    Biochemistry 36:5921-8. 1997
    ..Moreover, the FMN prosthetic group in SiR-FP23 and native SiR-FP is compared to that of cytochrome P450 reductase and bacterial cytochrome P450, which also contain one FAD and one FMN per polypeptide chain...
  50. ncbi 1.8 A crystal structure of the major NAD(P)H:FMN oxidoreductase of a bioluminescent bacterium, Vibrio fischeri: overall structure, cofactor and substrate-analog binding, and comparison with related flavoproteins
    H Koike
    Biotechnology Research Center, Graduate School of Science, University of Tokyo, Bunkyo ku, Tokyo 113 Japan
    J Mol Biol 280:259-73. 1998
    ..Upon binding of dicoumarol, most of the exposed re-face of the FMN cofactor is buried, which is consistent with the ping pong bi bi catalytic mechanism...
  51. ncbi The 1.59 A resolution crystal structure of TM0096, a flavin mononucleotide binding protein from Thermotoga maritima
    Frances Park
    Structural GenomiX, Inc, San Diego, CA 92121, USA
    Proteins 55:772-4. 2004
  52. ncbi The interaction of NADPH-P450 reductase with P450: an electrochemical study of the role of the flavin mononucleotide-binding domain
    R W Estabrook
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75235 9038, USA
    Arch Biochem Biophys 333:308-15. 1996
    ..It is speculated that a domain composed of acidic amino acids, located near the flavin mononucleotide-binding region of the flavoproteins, may serve as this site...
  53. ncbi Selection of carbon nanotubes with specific chiralities using helical assemblies of flavin mononucleotide
    Sang Yong Ju
    Nanomaterials Optoelectronics Laboratory NOEL, Polymer Program, University of Connecticut, Storrs, Connecticut 06269 3136, USA
    Nat Nanotechnol 3:356-62. 2008
    ..Here we show that the flavin mononucleotide, a common redox cofactor, wraps around single-walled carbon nanotubes in a helical pattern that imparts ..
  54. ncbi Synthesis and redox behavior of flavin mononucleotide-functionalized single-walled carbon nanotubes
    Sang Yong Ju
    Nanomaterials Optoelectronics Laboratory, Polymer Program, Department of Chemistry, Institute of Materials Science, University of Connecticut, Storrs, Connecticut 06269 3136, USA
    J Am Chem Soc 130:655-64. 2008
    In this contribution, we describe the synthesis and covalent attachment of an analogue of the flavin mononucleotide (FMN) cofactor onto carboxylic functionalities of single-walled carbon nanotubes (SWNTs)...
  55. pmc Flavin mononucleotide-binding flavoprotein family in the domain Archaea
    Yan Huai R Ding
    Department of Biochemistry and Molecular Biology, Eberly College of Science, The Pennsylvania State University, University Park, Pennsylvania 16802 4500, USA
    J Bacteriol 186:90-7. 2004
    ..A search of the databases suggests that AfpA is the prototype of a previously unrecognized flavoprotein family unique to the domain Archaea for which the name archaeoflavoprotein is proposed...
  56. ncbi Control of oxidation-reduction potentials in flavodoxin from Clostridium beijerinckii: the role of conformation changes
    M L Ludwig
    Department of Biological Chemistry, University of Michigan, Ann Arbor 48109 1055, USA
    Biochemistry 36:1259-80. 1997
    ..0 kcal/mol) and to increase the sq/hq potential by 80 mV (-1.7 kcal/mol). A thermodynamic scheme is introduced as a framework for rationalizing the properties of wild-type flavodoxin and the effects of the mutations...
  57. ncbi Mechanism of coenzyme binding to human methionine synthase reductase revealed through the crystal structure of the FNR-like module and isothermal titration calorimetry
    Kirsten R Wolthers
    Faculty of Life Sciences, University of Manchester, Manchester Interdisciplinary Biocentre, 131 Princess Street, Manchester M1 7DN, United Kingdom
    Biochemistry 46:11833-44. 2007
    ..The biological implications of an attenuated mechanism of MS reactivation by MSR on methionine and folate metabolism are discussed...
  58. ncbi Effect of inferior-segment Intacs with and without C3-R on keratoconus
    Colin C K Chan
    J Cataract Refract Surg 33:75-80. 2007
    ..To determine whether corneal collagen cross-linking with riboflavin (C3-R) augments the effect of inferior-segment Intacs (Addition Technology) in the treatment of keratoconus...
  59. pmc Structure and properties of recombinant human pyridoxine 5'-phosphate oxidase
    Faik N Musayev
    Department of Medicinal Chemistry, Institute for Structural Biology and Drug Discovery, Virginia Commonwealth University, 800 E Leigh Street, Richmond, VA 23219, USA
    Protein Sci 12:1455-63. 2003
    ..As observed with the E. coli enzyme, the human enzyme binds one molecule of pyridoxal 5'-phosphate tightly on each subunit...
  60. ncbi X-ray structure of Escherichia coli pyridoxine 5'-phosphate oxidase complexed with FMN at 1.8 A resolution
    M K Safo
    Institute for Structural Biology and Drug Discovery, Virginia Commonwealth University, Richmond, VA 23219, USA
    Structure 8:751-62. 2000
    ..PNPOx is a homodimeric enzyme with one flavin mononucleotide (FMN) molecule non-covalently bound to each subunit...
  61. ncbi Roseoflavin is a natural antibacterial compound that binds to FMN riboswitches and regulates gene expression
    Elaine R Lee
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA
    RNA Biol 6:187-94. 2009
    ..The Gram-positive bacterium Bacillus subtilis has two representatives of a class of riboswitches that bind flavin mononucleotide (FMN)...
  62. pmc Flavin mononucleotide (FMN)-based fluorescent protein (FbFP) as reporter for gene expression in the anaerobe Bacteroides fragilis
    Leandro A Lobo
    Instituto de Microbiologia Paulo de Gois, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
    FEMS Microbiol Lett 317:67-74. 2011
    In this study, we show the expression of flavin mononucleotide-based fluorescent protein (FbFP) BS2 as a marker for gene expression in the opportunistic human anaerobic pathogen Bacteroides fragilis...
  63. pmc FQR1, a novel primary auxin-response gene, encodes a flavin mononucleotide-binding quinone reductase
    Marta J Laskowski
    Department of Biology, Williams College, Williamstown, MA 01267, USA
    Plant Physiol 128:578-90. 2002
    FQR1 is a novel primary auxin-response gene that codes for a flavin mononucleotide-binding flavodoxin-like quinone reductase...
  64. pmc Kinetic limitation and cellular amount of pyridoxine (pyridoxamine) 5'-phosphate oxidase of Escherichia coli K-12
    G Zhao
    Department of Microbiology and Molecular Genetics, University of Texas, Houston Medical School 77030
    J Bacteriol 177:883-91. 1995
    ..The purified PdxH enzyme is a thermally stable 51-kDa homodimer containing one molecule of flavin mononucleotide (FMN)...
  65. ncbi Crystal structure of the FAD/NADPH-binding domain of rat neuronal nitric-oxide synthase. Comparisons with NADPH-cytochrome P450 oxidoreductase
    J Zhang
    Department of Biochemistry, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Biol Chem 276:37506-13. 2001
    ....
  66. ncbi Structural organization of WrbA in apo- and holoprotein crystals
    Julie Wolfova
    Institute of Physical Biology, University of South Bohemia, Zamek 136, 37333 Nove Hrady, Czech Republic
    Biochim Biophys Acta 1794:1288-98. 2009
    ..The results suggest that WrbAs are not a remote and unusual branch of the flavodoxin family as previously thought but rather a central member with unifying structural features...
  67. ncbi The structure of Escherichia coli nitroreductase complexed with nicotinic acid: three crystal forms at 1.7 A, 1.8 A and 2.4 A resolution
    A L Lovering
    School of Biosciences, University of Birmingham, UK
    J Mol Biol 309:203-13. 2001
    ..Analysis of the structure explains the broad substrate specificity of the enzyme, and provides the basis for rational design of novel prodrugs and for site-directed mutagenesis for improved enzyme activity...
  68. pmc A novel reduced flavin mononucleotide-dependent methanesulfonate sulfonatase encoded by the sulfur-regulated msu operon of Pseudomonas aeruginosa
    M A Kertesz
    Institute of Microbiology, Swiss Federal Institute of Technology, ETH Zentrum, CH 8092 Zurich, Switzerland
    J Bacteriol 181:1464-73. 1999
    ..aeruginosa PAO1 and sequenced. It encoded a 381-amino-acid protein that was related to several reduced flavin mononucleotide (FMNH2)-dependent monooxygenases, and it was the second in an operon of three genes, which we have named ..
  69. ncbi Role of the conserved phenylalanine 181 of NADPH-cytochrome P450 oxidoreductase in FMN binding and catalytic activity
    M J Paine
    ICRF Molecular Pharmacology Unit, Biomedical Research Centre, University of Dundee, Ninewells Hospital and Medical School, Dundee DD1 9SY, UK
    Biochemistry 40:13439-47. 2001
    ..The results indicate that Phe181 cooperates with Tyr140 and Tyr178 to play a major role in the binding and stability of FMN...
  70. ncbi Expression, purification, and characterization of recombinant Escherichia coli pyridoxine 5'-phosphate oxidase
    M di Salvo
    Institute for Structural Biology and Drug Discovery, Virginia Commonwealth University, Richmond, Virginia, 23298, USA
    Protein Expr Purif 13:349-56. 1998
    ..Using this value and the spectral properties of the bound FMN it was shown by three different methods that the dimeric enzyme contains two molecules of bound FMN per dimer and not one FMN as previously reported...
  71. pmc NADPH-cytochrome P450 oxidoreductase from the mosquito Anopheles minimus: kinetic studies and the influence of Leu86 and Leu219 on cofactor binding and protein stability
    Songklod Sarapusit
    Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Arch Biochem Biophys 477:53-9. 2008
    ..Analysis of the single mutants indicates that the proline to alanine substitution has no impact, but that both leucine to phenylalanine substitutions are essential for FMN binding and maximum stability of the enzyme...
  72. ncbi Determination of the redox properties of human NADPH-cytochrome P450 reductase
    A W Munro
    Department of Pure and Applied Chemistry, University of Strathclyde, The Royal College, 204 George Street, Glasgow, G1 1XL, UK
    Biochemistry 40:1956-63. 2001
    ..However, they differ markedly from those of yeast and bacterial CPRs, pointing to an important evolutionary difference in electronic regulation of these enzymes...
  73. pmc Covalent attachment of flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN) to enzymes: the current state of affairs
    M Mewies
    Department of Biochemistry, University of Leicester, UK
    Protein Sci 7:7-20. 1998
    ..Case studies are presented for a variety of covalent flavoenzymes, from which general findings are beginning to emerge...
  74. ncbi Relaxation kinetics of cytochrome P450 reductase: internal electron transfer is limited by conformational change and regulated by coenzyme binding
    Aldo Gutierrez
    Biological NMR Centre, University of Leicester, Medical Sciences Building, P O Box 138, University Road, Leicester LE1 9HN, UK
    Biochemistry 41:4626-37. 2002
    ..The studies reveal altered redox properties of the FAD in W676H CPR. The data are discussed in the context of previous stopped-flow studies of human CPR and the X-ray crystallographic structure of rat CPR...
  75. pmc Regulation of riboflavin biosynthesis in Bacillus subtilis is affected by the activity of the flavokinase/flavin adenine dinucleotide synthetase encoded by ribC
    M Mack
    Biotechnology Section, Vitamin Division, Hoffmann La Roche Ltd, Basel, Switzerland
    J Bacteriol 180:950-5. 1998
    ..The mutation ribC820 leads to deregulation of riboflavin biosynthesis in B. subtilis, most likely by preventing the accumulation of the effector molecule FMN or FAD...
  76. pmc Idiosyncratically tuned switching behavior of riboswitch aptamer domains revealed by comparative small-angle X-ray scattering analysis
    Nathan J Baird
    Howard Hughes Medical Institute, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109 1024, USA
    RNA 16:598-609. 2010
    ..domains of four different riboswitches that bind, respectively, thiamine pyrophosphate (TPP), flavin mononucleotide (FMN), lysine, and S-adenosyl methionine (SAM)...
  77. ncbi Crystal structure of human riboflavin kinase reveals a beta barrel fold and a novel active site arch
    Subramanian Karthikeyan
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Structure 11:265-73. 2003
    ..The invariant residues Asn36 and Glu86 are implicated in the catalysis...
  78. ncbi Reporter proteins for in vivo fluorescence without oxygen
    Thomas Drepper
    Institute of Molecular Enzyme Technology, Heinrich Heine University Duesseldorf, Research Center Juelich, Stetternicher Forst, D 52426 Juelich, Germany
    Nat Biotechnol 25:443-5. 2007
    ..Bacillus subtilis and Pseudomonas putida that contain light-oxygen-voltage-sensing domains, we engineered flavin mononucleotide-based fluorescent proteins that can be used as fluorescent reporters in both aerobic and anaerobic ..
  79. ncbi Mutagenic analysis of an invariant aspartate residue in chorismate synthase supports its role as an active site base
    Gernot Rauch
    Institute of Biochemistry, Graz University of Technology, Graz, Austria
    Biochemistry 46:3768-74. 2007
    ....
  80. pmc The bifunctional flavokinase/flavin adenine dinucleotide synthetase from Streptomyces davawensis produces inactive flavin cofactors and is not involved in resistance to the antibiotic roseoflavin
    Simon Grill
    Institut für Technische Mikrobiologie, Hochschule Mannheim, Windeckstr 110, 68163 Mannheim, Germany
    J Bacteriol 190:1546-53. 2008
    ..3 10(-2) microM(-1) s(-1)). Both RibC enzymes synthesized RoFAD and RoFMN. The functional expression of S. davawensis ribC did not confer roseoflavin resistance to a ribC-defective B. subtilis strain...
  81. ncbi Molecular determinants for FMN-binding in Desulfovibrio gigas flavoredoxin
    Manuela Broco
    Instituto de Tecnologia Química e Biológica ITQB, Universidade Nova de Lisboa, Av Republica EAN, 2784 505 Oeiras, Portugal
    FEBS Lett 581:4397-402. 2007
    ..These findings were correlated with the protein structure in order to contribute to a better understanding of the structure-function relationships in flavoredoxin...
  82. ncbi A structural model for chorismate synthase from Mycobacterium tuberculosis in complex with coenzyme and substrate
    Claudia Lemelle Fernandes
    Laboratório de Bioinformática, Modelagem e Simulação de Biossistemas LABIO, Faculdade de Informática, PUCRS, Brazil
    Comput Biol Med 37:149-58. 2007
    ..However, novel interactions involving ARG111, GLY113 and SER317 were also observed...
  83. pmc Coenzyme recognition and gene regulation by a flavin mononucleotide riboswitch
    Alexander Serganov
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA
    Nature 458:233-7. 2009
    The biosynthesis of several protein cofactors is subject to feedback regulation by riboswitches. Flavin mononucleotide (FMN)-specific riboswitches, also known as RFN elements, direct expression of bacterial genes involved in the ..
  84. ncbi Crystal structure of Schizosaccharomyces pombe riboflavin kinase reveals a novel ATP and riboflavin-binding fold
    Stefanie Bauer
    Max Planck Institut fur Biochemie, Abteilung Strukturforschung, Am Klopferspitz 18a, D 82152, Martinsried, Germany
    J Mol Biol 326:1463-73. 2003
    ..The unusual metal-binding site involves, in addition to the ADP phosphates, only the strictly conserved Thr45. This may explain the preference for zinc observed in vitro...
  85. pmc Identification of a 14mer RNA that recognizes and binds flavin mononucleotide with high affinity
    Peter C Anderson
    School of Pharmacy, University of Wisconsin, 777 Highland Avenue, Madison, WI 53705, USA
    Nucleic Acids Res 33:6992-9. 2005
    ..Here, we show that a 35mer RNA aptamer for the cofactor flavin mononucleotide (FMN) identified by in vitro evolution can be computationally evolved to a mere 14mer structure containing ..
  86. ncbi Structures of Mycobacterium tuberculosispyridoxine 5'-phosphate oxidase and its complexes with flavin mononucleotide and pyridoxal 5'-phosphate
    Bichitra K Biswal
    CIHR Group in Protein Structure and Function, Department of Biochemistry, University of Alberta, Edmonton T6G 2H7, Canada
    Acta Crystallogr D Biol Crystallogr 61:1492-9. 2005
    ..The structures of flavin mononucleotide (FMN) and pyridoxal 5'-phosphate (PLP) bound separately to Rv1155 have been determined at 2.2 and 1...
  87. ncbi Entropy changes drive the electron transfer reaction of triplet flavin mononucleotide from aromatic amino acids in cation-organized aqueous media. A laser-induced optoacoustic study
    Luis Crovetto
    Max Planck Institut für Bioanorganische Chemie formerly Strahlenchemie, Mulheim an der Ruhr, Germany
    Photochem Photobiol 82:281-90. 2006
    ....
  88. ncbi Crystal structure of chorismate synthase: a novel FMN-binding protein fold and functional insights
    Hyung Jun Ahn
    Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 151 0742, South Korea
    J Mol Biol 336:903-15. 2004
    ..The isoalloxazine ring of the bound FMN is significantly non-planar. Our structure illuminates the essential functional roles played by the cofactor...
  89. ncbi Genetic control by metabolite-binding riboswitches
    Wade C Winkler
    Department of Molecular, Cellular, and Developmental Biology, Yale University, Kline Biology Tower 506, 266 Whitney Avenue, New Haven, CT 06520, USA
    Chembiochem 4:1024-32. 2003
  90. pmc An mRNA structure that controls gene expression by binding FMN
    Wade C Winkler
    Department of Molecular, Cellular, and Developmental Biology, Yale University, P O Box 208103, New Haven, CT 06520 8103, USA
    Proc Natl Acad Sci U S A 99:15908-13. 2002
    ..domain that is found frequently in the 5'-untranslated regions of prokaryotic mRNAs that encode for flavin mononucleotide (FMN) biosynthesis and transport proteins...
  91. ncbi Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase
    D S Bredt
    Department of Neuroscience, Johns Hopkins Medical Institutions, Baltimore, Maryland 21205
    Nature 351:714-8. 1991
    ..Cloning of a complementary DNA for brain nitric oxide synthase reveals recognition sites for NADPH, FAD, flavin mononucleotide and calmodulin as well as phosphorylation sites, indicating that the synthase is regulated by many ..
  92. ncbi Binding of the oxidized, reduced, and radical flavin species to chorismate synthase. An investigation by spectrophotometry, fluorimetry, and electron paramagnetic resonance and electron nuclear double resonance spectroscopy
    P Macheroux
    Nitrogen Fixation Laboratory, John Innes Centre, Norwich Research Park, UK
    Biochemistry 35:1643-52. 1996
    ....
  93. ncbi Purification and characterization of NfrA1, a Bacillus subtilis nitro/flavin reductase capable of interacting with the bacterial luciferase
    S Zenno
    Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Japan
    Biosci Biotechnol Biochem 62:1978-87. 1998
    ....
  94. ncbi Spectroscopic and kinetic characterization of the bifunctional chorismate synthase from Neurospora crassa: evidence for a common binding site for 5-enolpyruvylshikimate 3-phosphate and NADPH
    K Kitzing
    , , , , Switzerland
    J Biol Chem 276:42658-66. 2001
    ....
  95. pmc Purification, characterization, and overexpression of flavin reductase involved in dibenzothiophene desulfurization by Rhodococcus erythropolis D-1
    T Matsubara
    Department of Biotechnology, Tottori University, Tottori 680-8552, Japan
    Appl Environ Microbiol 67:1179-84. 2001
    ..The enzyme catalyzed NADH-dependent reduction of flavin mononucleotide (FMN), and the K(m) values for NADH and FMN were 208 and 10.8 microM, respectively...
  96. ncbi NAD(P)H-flavin oxidoreductase from the bioluminescent bacterium, Vibrio fischeri ATCC 7744, is a flavoprotein
    S Inouye
    Yokohama Research Center, Chisso Corp, Japan
    FEBS Lett 347:163-8. 1994
    ..The purified enzyme had a typical flavoprotein absorption spectrum and flavin mononucleotide (FMN) was identified as a prosthetic group, non-covalently bound in a molar ratio of 1:1...
  97. ncbi Structural analysis of interactions for complex formation between Ferredoxin-NADP+ reductase and its protein partners
    Tomas Mayoral
    Grupo de Cristalografia Macromolecular y Biologia Estructural, Instituto Quimica Fisica Rocasolano, C S I C Serrano 119, 28006 Madrid, Spain
    Proteins 59:592-602. 2005
    ....
  98. pmc Covalent binding of flavins to RnfG and RnfD in the Rnf complex from Vibrio cholerae
    Julianne Backiel
    Department of Biology and Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, 110 Eight Street, Troy, New York 12180, USA
    Biochemistry 47:11273-84. 2008
    ..In contrast, in NqrC and NqrB the flavins are located in a cytoplasmic loop. This topological analysis suggests that there may be mechanistic differences between the Rnf and Na+-NQR complexes...
  99. ncbi Interflavin one-electron transfer in the inducible nitric oxide synthase reductase domain and NADPH-cytochrome P450 reductase
    Keita Yamamoto
    Graduate School of Life Science, Himeji Institute of Technology, University of Hyogo, 3 2 1 Kouto, Kamigori cho, Hyogo 678 1297, Japan
    Arch Biochem Biophys 440:65-78. 2005
    ..On the basis of these data, we propose a common model for the catalytic cycle of both CaM-bound iNOS reductase domain and CPR...
  100. pmc Structural analysis of FAD synthetase from Corynebacterium ammoniagenes
    Susana Frago
    Departamento de Bioquimica y Biologia Molecular y Celular, Facultad de Ciencias and Institute of Biocomputation and Physics of Complex Systems, Universidad de Zaragoza, Zaragoza, Spain
    BMC Microbiol 8:160. 2008
    ..The prokaryotic FAD synthetase family - a group of bifunctional enzymes that catalyse riboflavin phosphorylation and FMN adenylylation within a single polypeptide chain- was analysed in terms of sequence and structure...
  101. ncbi Versatile regulation of neuronal nitric oxide synthase by specific regions of its C-terminal tail
    Mauro Tiso
    Department of Pathobiology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Biochemistry 46:14418-28. 2007
    ..Our results reveal that the CT exerts both multifaceted and regiospecific effects on catalytic activities and related behaviors, and thus provide new insights into mechanisms that regulate nNOS catalysis...

Research Grants26

  1. Flavoenzymes in Pyrimidine Metabolism
    Bruce A Palfey; Fiscal Year: 2011
    ..Our studies of the rates of the chemical reactions will identify important parts of the proteins, how they move during reactions, how they speed the synthesis of products, and how these reactions might be blocked. ..
  2. Nitrite Mediated Cardioprotection
    DAVID JOSEPH LEFER; Fiscal Year: 2012
    ..The synthesis of NO is critically influenced by various cofactors such as tetrahydrobiopterin, flavin mononucleotide and flavin adenine dinucleotide, the presence of reduced thiols, and the endogenous NOS inhibitor ..
  3. Pharmacogenomics of Human P450 Oxidoreductase
    Walter L Miller; Fiscal Year: 2012
    ..amino-acid, 78 kDa protein that contains two flavins, a flavin adenine dinucleotide moiety (FAD), and a flavin mononucleotide (FMN). There is substantial genetic variation in the hepatic P450 enzymes...
  4. Real-time detection of cancer biomarkers using a self-sustained aptazyme
    Charles Olea; Fiscal Year: 2013
    ..The aptazyme has been shown to interchangeably detect theophylline and flavin mononucleotide (FMN) within physiological ranges...
  5. Non-coding RNA Structure through a Mutate-and-Map Strategy
    Rhiju Das; Fiscal Year: 2013
    ....
  6. Structural Basis for Isoprenoid Biosynthesis
    JOSEPH NOEL; Fiscal Year: 2006
    ..This type 2 IPP isomerase, unlike the well-studied type 1 enzymes, depends on flavin mononucleotide and NADPH for activity...
  7. Biosynthesis and Physiology of Vitamin B12 in Prokaryotes
    MICHIKO TAGA; Fiscal Year: 2009
    ..and biochemical analyses demonstrated that BluB catalyzes the oxygen-dependent transformation of flavin mononucleotide (FMN) to DMB...
  8. Mechanistic Studies of Type II IPP Isomerase
    Steven Rothman; Fiscal Year: 2006
    A recent report describes the cloning and preliminary characterization of an unusual flavin mononucleotide (FMN) dependent enzyme, the type II isopentenyl diphosphate (IPP) isomerase...
  9. COFACTOR ROLE IN BETA-SHEET PROTEIN FOLDING
    Pernilla Wittung Stafshede; Fiscal Year: 2005
    ..a copper-ion cofactor, flavodoxin, a protein with an a/b doubly-wound topology coordinating an organic flavin mononucleotide (FMN), and cytochrome f, a b-sheet protein covalently linked to a heme...
  10. RNA APTAMER COMPLEXES--STRUCTURES AND INTERACTIONS
    Dinshaw Patel; Fiscal Year: 1999
    ..Preliminary NMR data are presented on RNA aptamers complexed to flavin mononucleotide, adenosine triphosphate and the aminoglycoside antibiotic tobramycin that exhibit extremely well resolved ..
  11. Regulation of Nitric Oxide Synthase through Formation of the Output State
    Changjian Feng; Fiscal Year: 2009
    ..It is proposed that CaM activates NO synthesis in eNOS and nNOS through a conformational change of the flavin mononucleotide (FMN) domain from its shielded electron-accepting (input) state to a new electron-donating (output) state...
  12. Energetics, kinetics and mechanism in gene regulatory processes
    DONALD CROTHERS; Fiscal Year: 2009
    ..Riboswitches that respond to flavin mononucleotide (FMN), adenine, and glycine are the current targets of study...
  13. NITROGENASE MECHANISM AND ASSEMBLY
    Thomas Poulos; Fiscal Year: 2002
    ..These studies are expected to have an important impact on our understanding of the general issue of how preformed metal clusters can be inserted into proteins. ..
  14. 7 FE FDI AS A MODEL FE-S AND REGULATORY PROTEIN
    Thomas Poulos; Fiscal Year: 2002
    ..g. superoxide, NO). ..
  15. BIOSYNTHESIS AND METABOLISM OF CYCLOPENTANOIDS
    Ronald Parry; Fiscal Year: 2001
    ..The potential use of the CmaA gene for construction of hybrid peptide antibiotics containing L-alloisoleucine or coronamic acid will also be explored, using surfactin biosynthesis in B. subtilis as a model system. ..
  16. Molecular Targets of Novel Antifungal Compounds
    Ameeta Agarwal; Fiscal Year: 2008
    ..This work will provide a starting point in the development of new antifungal therapies. [unreadable] [unreadable] [unreadable]..
  17. BIOSYNTHESIS OF SOME MICROBIAL METABOLITES
    Ronald Parry; Fiscal Year: 2007
    ..Future investigations of sparsomycin will focus on cloning the sparsomycin resistance gene from S. sparsogenes to understand the mechanism of self-resistance in this organism and to gain access to the biosynthetic genes. ..
  18. Ethanol effects on liver in self-administering primates
    Carol Cunningham; Fiscal Year: 2004
    ..fascicularis will develop liver pathology past the fatty liver stage and 2) to evaluate the efficacy of using blood and urine samples to follow development of alcoholic liver disease in an animal model. ..
  19. CHRONIC ALCOHOLISM AND LIVER ENERGY AND LIPID METABOLISM
    Carol Cunningham; Fiscal Year: 2004
    ..Phosphorus NMR spectroscopy will be utilized heavily in these latter studies. ..
  20. Prostaglandin 19-& 20-Hydroxylation by Cytochrome P450
    Bettie Sue Masters; Fiscal Year: 2005
    ..abstract_text> ..
  21. Single Molecule Studies of Enzyme Catalysis
    Gordon Hammes; Fiscal Year: 2005
    ..These experiments are directed at understanding better how enzymes catalyze physiological reactions. A better understanding of enzyme mechanisms is essential for understanding the physiology of disease and the development of new drugs. ..
  22. STRUCTURAL DETERMINANTS OF FAD and FMN-REQUIRING ENZYMES
    Bettie Sue Masters; Fiscal Year: 2005
    ..Upon determination of the unique structural features in the flavoprotein domains of constitutive isoforms in comparison with inducible NOS and CYPOR, a basis for drug design could develop. ..
  23. THERMODYNAMICS OF BIOCHEMICAL SYSTEMS
    ROBERT ALBERTY; Fiscal Year: 2005
    ..This quantitative treatment of the literature data on biochemical reactions will uncover errors because deltafG'0 values can be determined by use of two or more biochemical reactions, which should give the same answer. ..
  24. BIOCHEMISTRY OF NITRIC OXIDE SYNTHESIS
    Dennis J Stuehr; Fiscal Year: 2010
    ..Relevance: By clarifying how nitric oxide production occurs and is regulated, our work may help to develop treatments for human diseases that involve making too much or too little nitric oxide. ..
  25. DNA-based Arrays of Cross-reactive Molecular Sensors
    Milan Stojanovic; Fiscal Year: 2004
    ..In the next phase, DNA-based cross-reactive sensors for oligosaccharides will be incorporated into arrays to expand the panel of metabolites that could be profiled in a single-step assay. ..
  26. Protein Expression, Nitration and Gene Changes in ALS
    Dennis Stuehr; Fiscal Year: 2002
    ..These include markers for disease progression, treatment efficacy, and high-throughput drug discovery. Our work with non-neuronal tissue may identify markers that provide far more-convenient monitoring. ..