flavin mononucleotide

Summary

Summary: A coenzyme for a number of oxidative enzymes including NADH DEHYDROGENASE. It is the principal form in which RIBOFLAVIN is found in cells and tissues.

Top Publications

  1. ncbi The FAD-shielding residue Phe1395 regulates neuronal nitric-oxide synthase catalysis by controlling NADP+ affinity and a conformational equilibrium within the flavoprotein domain
    David W Konas
    Department of Immunology, The Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    J Biol Chem 279:35412-25. 2004
  2. doi Flavogenomics--a genomic and structural view of flavin-dependent proteins
    Peter Macheroux
    Institute of Biochemistry, Graz University of Technology, Graz, Austria
    FEBS J 278:2625-34. 2011
  3. ncbi The speed of RNA transcription and metabolite binding kinetics operate an FMN riboswitch
    J Kenneth Wickiser
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
    Mol Cell 18:49-60. 2005
  4. pmc Flavin mononucleotide-based fluorescent reporter proteins outperform green fluorescent protein-like proteins as quantitative in vivo real-time reporters
    Thomas Drepper
    Institute of Molecular Enzyme Technology, Heinrich Heine University Duesseldorf, Research Center Juelich, Stetternicher Forst, D 52426 Juelich, Germany
    Appl Environ Microbiol 76:5990-4. 2010
  5. pmc Crystal structure of the flavoprotein ArsH from Sinorhizobium meliloti
    Jun Ye
    Department of Biochemistry and Molecular Biology, Wayne State University, School of Medicine, 540 East Canfield Avenue, Detroit, MI 48201, USA
    FEBS Lett 581:3996-4000. 2007
  6. pmc A crystallographic study of Cys69Ala flavodoxin II from Azotobacter vinelandii: structural determinants of redox potential
    Sharmini Alagaratnam
    Leiden Institute of Chemistry, P O Box 9502, 2300 RA Leiden, The Netherlands
    Protein Sci 14:2284-95. 2005
  7. pmc Genetic transformation of an obligate anaerobe, P. gingivalis for FMN-green fluorescent protein expression in studying host-microbe interaction
    Chul Hee Choi
    Department of Periodontology, University of Florida, Gainesville, Florida, United States of America
    PLoS ONE 6:e18499. 2011
  8. pmc Flavin mononucleotide (FMN)-based fluorescent protein (FbFP) as reporter for gene expression in the anaerobe Bacteroides fragilis
    Leandro A Lobo
    Instituto de Microbiologia Paulo de Gois, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
    FEMS Microbiol Lett 317:67-74. 2011
  9. pmc A novel chromate reductase from Thermus scotoductus SA-01 related to old yellow enzyme
    Diederik Johannes Opperman
    Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, Bloemfontein 9300, South Africa
    J Bacteriol 190:3076-82. 2008
  10. pmc Structure determination of an FMN reductase from Pseudomonas aeruginosa PA01 using sulfur anomalous signal
    Rakhi Agarwal
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA
    Acta Crystallogr D Biol Crystallogr 62:383-91. 2006

Research Grants

  1. STRUCTURAL STUDIES ON NITRIC OXIDE SYNTHASE
    Thomas Poulos; Fiscal Year: 2001
  2. Flavoenzymes in Pyrimidine Metabolism
    Bruce A Palfey; Fiscal Year: 2011
  3. Pharmacogenomics of Human P450 Oxidoreductase
    Walter L Miller; Fiscal Year: 2010
  4. Pharmacogenomics of Human P450 Oxidoreductase
    WALTER MILLER; Fiscal Year: 2009
  5. Structural Basis for Isoprenoid Biosynthesis
    JOSEPH NOEL; Fiscal Year: 2006
  6. Nitrite Mediated Cardioprotection
    DAVID JOSEPH LEFER; Fiscal Year: 2010
  7. Mechanistic Studies of Type II IPP Isomerase
    Steven Rothman; Fiscal Year: 2006
  8. COFACTOR ROLE IN BETA-SHEET PROTEIN FOLDING
    Pernilla Wittung Stafshede; Fiscal Year: 2005
  9. Energetics, kinetics and mechanism in gene regulatory processes
    DONALD CROTHERS; Fiscal Year: 2009
  10. Energetics, kinetics and mechanism in gene regulatory processes
    DONALD CROTHERS; Fiscal Year: 2007

Detail Information

Publications250 found, 100 shown here

  1. ncbi The FAD-shielding residue Phe1395 regulates neuronal nitric-oxide synthase catalysis by controlling NADP+ affinity and a conformational equilibrium within the flavoprotein domain
    David W Konas
    Department of Immunology, The Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    J Biol Chem 279:35412-25. 2004
    ....
  2. doi Flavogenomics--a genomic and structural view of flavin-dependent proteins
    Peter Macheroux
    Institute of Biochemistry, Graz University of Technology, Graz, Austria
    FEBS J 278:2625-34. 2011
    ..An understanding of 'flavin-intensive' lifestyles, such as in the human pathogen Mycobacterium tuberculosis, may result in valuable new intervention strategies that target either riboflavin biosynthesis or uptake...
  3. ncbi The speed of RNA transcription and metabolite binding kinetics operate an FMN riboswitch
    J Kenneth Wickiser
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520, USA
    Mol Cell 18:49-60. 2005
    ..A flavin mononucleotide (FMN)-dependent riboswitch from the ribDEAHT operon of Bacillus subtilis uses a transcription termination ..
  4. pmc Flavin mononucleotide-based fluorescent reporter proteins outperform green fluorescent protein-like proteins as quantitative in vivo real-time reporters
    Thomas Drepper
    Institute of Molecular Enzyme Technology, Heinrich Heine University Duesseldorf, Research Center Juelich, Stetternicher Forst, D 52426 Juelich, Germany
    Appl Environ Microbiol 76:5990-4. 2010
    ..Instead, flavin mononucleotide (FMN)-binding fluorescent proteins (FbFPs) are suitable for quantitative real-time in vivo assays under ..
  5. pmc Crystal structure of the flavoprotein ArsH from Sinorhizobium meliloti
    Jun Ye
    Department of Biochemistry and Molecular Biology, Wayne State University, School of Medicine, 540 East Canfield Avenue, Detroit, MI 48201, USA
    FEBS Lett 581:3996-4000. 2007
    ..The N- and C-terminal extensions of ArsH are involved in interactions between subunits and tetramer formation. The structure may provide insight in how ArsH participates in arsenic detoxification...
  6. pmc A crystallographic study of Cys69Ala flavodoxin II from Azotobacter vinelandii: structural determinants of redox potential
    Sharmini Alagaratnam
    Leiden Institute of Chemistry, P O Box 9502, 2300 RA Leiden, The Netherlands
    Protein Sci 14:2284-95. 2005
    ..The flavin mononucleotide (FMN) cofactor is flanked by a leucine on its re face instead of the more conserved tryptophan, resulting ..
  7. pmc Genetic transformation of an obligate anaerobe, P. gingivalis for FMN-green fluorescent protein expression in studying host-microbe interaction
    Chul Hee Choi
    Department of Periodontology, University of Florida, Gainesville, Florida, United States of America
    PLoS ONE 6:e18499. 2011
    The recent introduction of "oxygen-independent" flavin mononucleotide (FMN)-based fluorescent proteins (FbFPs) is of major interest to both eukaryotic and prokaryotic microbial biologists...
  8. pmc Flavin mononucleotide (FMN)-based fluorescent protein (FbFP) as reporter for gene expression in the anaerobe Bacteroides fragilis
    Leandro A Lobo
    Instituto de Microbiologia Paulo de Gois, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
    FEMS Microbiol Lett 317:67-74. 2011
    In this study, we show the expression of flavin mononucleotide-based fluorescent protein (FbFP) BS2 as a marker for gene expression in the opportunistic human anaerobic pathogen Bacteroides fragilis...
  9. pmc A novel chromate reductase from Thermus scotoductus SA-01 related to old yellow enzyme
    Diederik Johannes Opperman
    Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, Bloemfontein 9300, South Africa
    J Bacteriol 190:3076-82. 2008
    ..protein, with a monomer molecular mass of approximately 36 kDa, containing a noncovalently bound flavin mononucleotide cofactor. The chromate reductase is optimally active at a pH of 6...
  10. pmc Structure determination of an FMN reductase from Pseudomonas aeruginosa PA01 using sulfur anomalous signal
    Rakhi Agarwal
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA
    Acta Crystallogr D Biol Crystallogr 62:383-91. 2006
    ..this protein lacks the flavodoxin key fingerprint motif [(T/S)XTGXT], it has been confirmed to bind flavin mononucleotide and the binding site was identified via X-ray crystallography...
  11. ncbi Crystal structures of a novel ferric reductase from the hyperthermophilic archaeon Archaeoglobus fulgidus and its complex with NADP+
    H J Chiu
    Division of Chemistry and Chemical Engineering, California Institute of Technology, 91125, USA
    Structure 9:311-9. 2001
    ..However, the high cellular abundance of the A. fulgidus FeR (approximately 0.75% of the total soluble protein) suggests a catabolic role for this enzyme as the terminal electron acceptor in a ferric iron-based respiratory pathway [1]...
  12. pmc Photochemical properties of the flavin mononucleotide-binding domains of the phototropins from Arabidopsis, rice, and Chlamydomonas reinhardtii
    Masahiro Kasahara
    National Institute for Basic Biology, Okazaki 444 8585, Japan
    Plant Physiol 129:762-73. 2002
    ..or voltage (LOV) domains at their N termini (LOV1 and LOV2), each a binding site for the chromophore flavin mononucleotide (FMN). Their C termini contain a serine/threonine protein kinase domain...
  13. ncbi X-ray structure of Escherichia coli pyridoxine 5'-phosphate oxidase complexed with pyridoxal 5'-phosphate at 2.0 A resolution
    M K Safo
    Institute for Structural Biology and Drug Discovery, and Department of Biochemistry, Virginia Commonwealth University, Richmond, VA 23219, USA
    J Mol Biol 310:817-26. 2001
    ..A possible tunnel exists between the two sites so that pyridoxal 5'-phosphate formed at the active site may transfer to the non-catalytic site without passing though the solvent...
  14. pmc Flavin mononucleotide-based fluorescent protein as an oxygen-independent reporter in Candida albicans and Saccharomyces cerevisiae
    D Tielker
    Institut fur Mikrobiologie, Forschungszentrum Julich, Heinrich Heine Universitat Dusseldorf, Dusseldorf, Germany
    Eukaryot Cell 8:913-5. 2009
    Hypoxia is encountered frequently by pathogenic and apathogenic fungi. A codon-adapted gene encoding flavin mononucleotide-based fluorescent protein (CaFbFP) was expressed in Candida albicans and Saccharomyces cerevisiae...
  15. doi Structural organization of WrbA in apo- and holoprotein crystals
    Julie Wolfova
    Institute of Physical Biology, University of South Bohemia, Zamek 136, 37333 Nove Hrady, Czech Republic
    Biochim Biophys Acta 1794:1288-98. 2009
    ..The results suggest that WrbAs are not a remote and unusual branch of the flavodoxin family as previously thought but rather a central member with unifying structural features...
  16. pmc Molecular basis of reduced pyridoxine 5'-phosphate oxidase catalytic activity in neonatal epileptic encephalopathy disorder
    Faik N Musayev
    Department of Medicinal Chemistry, School of Pharmacy, and Institute for Structural Biology and Drug Discovery, Virginia Commonwealth University, Richmond, Virginia 23219, USA
    J Biol Chem 284:30949-56. 2009
    ..These results provide a molecular basis for the phenotype associated with the R229W mutation, as well as providing a foundation for understanding the pathophysiological consequences of pyridoxine 5'-phosphate oxidase mutations...
  17. ncbi Riboflavin, flavin mononucleotide, and flavin adenine dinucleotide in human plasma and erythrocytes at baseline and after low-dose riboflavin supplementation
    Steinar Hustad
    LOCUS for Homocysteine and Related Vitamins, University of Bergen, Armauer Hansens Hus, N 5021 Bergen, Norway
    Clin Chem 48:1571-7. 2002
    Vitamin B(2) exists in blood as riboflavin and its cofactors, flavin mononucleotide (FMN) and FAD. The erythrocyte glutathione reductase activation coefficient (EGRAC) has traditionally been used to assess vitamin B(2) status in humans...
  18. pmc Linear free energy relationships demonstrate a catalytic role for the flavin mononucleotide coenzyme of the type II isopentenyl diphosphate:dimethylallyl diphosphate isomerase
    Christopher J Thibodeaux
    Division of Medicinal Chemistry, College of Pharmacy, Department of Chemistry and Biochemistry, and Institute of Cellular and Molecular Biology, University of Texas, Austin, Texas 78712, USA
    J Am Chem Soc 132:9994-6. 2010
    ..Surprisingly, IDI-2 requires a reduced flavin mononucleotide (FMN) coenzyme to carry out this redox neutral isomerization...
  19. ncbi New frontiers in structural flavoenzymology
    Luigi De Colibus
    Dipartimento di Genetica e Microbiologia, Universita di Pavia, Via Ferrata 1, 27100 Pavia, Italy
    Curr Opin Struct Biol 16:722-8. 2006
    ..The integration of the enzymology data with crystallographic studies at atomic resolution is resulting in unprecedented insight into the chemical and geometric properties underlying flavoenzyme function...
  20. ncbi Biochemical characterization of WrbA, founding member of a new family of multimeric flavodoxin-like proteins
    R Grandori
    Department of Chemistry, Princeton University, Princeton, New Jersey 08544 1009, USA
    J Biol Chem 273:20960-6. 1998
    ..Although many proteins with flavodoxin-like domains are known to be multimers, WrbA is apparently the first characterized case in which multimerization is associated directly with the flavodoxin-like domain itself...
  21. pmc FQR1, a novel primary auxin-response gene, encodes a flavin mononucleotide-binding quinone reductase
    Marta J Laskowski
    Department of Biology, Williams College, Williamstown, MA 01267, USA
    Plant Physiol 128:578-90. 2002
    FQR1 is a novel primary auxin-response gene that codes for a flavin mononucleotide-binding flavodoxin-like quinone reductase...
  22. pmc BluB cannibalizes flavin to form the lower ligand of vitamin B12
    Michiko E Taga
    Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Nature 446:449-53. 2007
    ..Here we show that BluB triggers the unprecedented fragmentation and contraction of the bound flavin mononucleotide cofactor and cleavage of the ribityl tail to form DMB and D-erythrose 4-phosphate...
  23. ncbi A second FMN binding site in yeast NADPH-cytochrome P450 reductase suggests a mechanism of electron transfer by diflavin reductases
    David C Lamb
    Wolfson Laboratory of P450 Biodiversity, Swansea Medical School University of Wales Swansea, Swansea, Wales SA2 8PP, UK
    Structure 14:51-61. 2006
    ....
  24. ncbi Flavin mononucleotide-binding domain of the flavoprotein component of the sulfite reductase from Escherichia coli
    J Coves
    Laboratoire d Etudes Dynamiques et Structurales de la Sélectivité, Unité Mixte de Recherche du Centre National de la Recherche Scientifique 5616, Universite Joseph Fourier, Grenoble, France
    Biochemistry 36:5921-8. 1997
    ..Moreover, the FMN prosthetic group in SiR-FP23 and native SiR-FP is compared to that of cytochrome P450 reductase and bacterial cytochrome P450, which also contain one FAD and one FMN per polypeptide chain...
  25. pmc The riboflavin analog roseoflavin targets an FMN-riboswitch and blocks Listeria monocytogenes growth, but also stimulates virulence gene-expression and infection
    Mikael Mansjö
    Department of Molecular Biology, Umea University, Umea, Sweden
    RNA Biol 8:674-80. 2011
    ..monocytogenes virulence gene expression and infection abilities in a mechanism independent of the FMN riboswitch. Our results suggest that roseoflavin can block growth but also enhance Listeria virulence...
  26. ncbi Photo-reduction of flavin mononucleotide to semiquinone form in LOV domain mutants of blue-light receptor phot from Chlamydomonas reinhardtii
    S H Song
    Institut fur Physikalische und Theoretische Chemie, Universitat Regensburg, D 93053 Regensburg, Germany
    J Photochem Photobiol B 87:37-48. 2007
    ..Blue-light exposure reduced the fully oxidized flavin mononucleotide, FMN(ox), to FMN semiquinone, FMNH*, (quantum efficiency around 1%) which further reduced to FMN ..
  27. ncbi 1.8 A crystal structure of the major NAD(P)H:FMN oxidoreductase of a bioluminescent bacterium, Vibrio fischeri: overall structure, cofactor and substrate-analog binding, and comparison with related flavoproteins
    H Koike
    Biotechnology Research Center, Graduate School of Science, University of Tokyo, Bunkyo ku, Tokyo 113 Japan
    J Mol Biol 280:259-73. 1998
    ..Upon binding of dicoumarol, most of the exposed re-face of the FMN cofactor is buried, which is consistent with the ping pong bi bi catalytic mechanism...
  28. pmc Crystal structure of ChrR--a quinone reductase with the capacity to reduce chromate
    Subramaniam Eswaramoorthy
    Department of Biology, Brookhaven National Laboratory, Upton, New York, USA
    PLoS ONE 7:e36017. 2012
    ..2 Å resolution, shows that it belongs to the flavodoxin superfamily in which flavin mononucleotide (FMN) is firmly anchored to the protein...
  29. ncbi Biochemistry: molecular cannibalism
    Steven E Ealick
    Nature 446:387-8. 2007
  30. pmc Staphylococcus aureus NfrA (SA0367) is a flavin mononucleotide-dependent NADPH oxidase involved in oxidative stress response
    Karin Streker
    Institut für Molekulare Infektionsbiologie, Rontgenring 11, D 97070 Wurzburg, Germany
    J Bacteriol 187:2249-56. 2005
    ..aureus. The purified protein oxidized NADPH but not NADH, producing NADP in the presence of flavin mononucleotide, suggesting that NfrA is an NADPH oxidase in S. aureus...
  31. ncbi Redox properties of the isolated flavin mononucleotide- and flavin adenine dinucleotide-binding domains of neuronal nitric oxide synthase
    Pierre E Garnaud
    School of Chemistry, University of Edinburgh, King s Buildings, West Mains Road, Edinburgh EH9 3JJ, UK
    Biochemistry 43:11035-44. 2004
    ..Here we report the preparation of the isolated flavin mononucleotide (FMN)-binding domain of nNOS with bound CaM and the electrochemical analysis of this and the isolated ..
  32. ncbi Spectroscopic characterization of flavin mononucleotide bound to the LOV1 domain of Phot1 from Chlamydomonas reinhardtii
    W Holzer
    Institut II Experimentelle und Angewandte Physik, Universitat Regensburg, Germany
    Photochem Photobiol 75:479-87. 2002
    The absorption and emission behavior of flavin mononucleotide (FMN) in the light-, oxygen- and voltage-sensitive (LOV) domain LOV1 of the photoreceptor Phot1 from the green alga Chlamydomonas reinhardtii was studied...
  33. ncbi Sensing small molecules by nascent RNA: a mechanism to control transcription in bacteria
    Alexander S Mironov
    State Research Institute of Genetics and Selection of Industrial Microorganisms, 113545, Moscow, Russia
    Cell 111:747-56. 2002
    Thiamin and riboflavin are precursors of essential coenzymes-thiamin pyrophosphate (TPP) and flavin mononucleotide (FMN)/flavin adenine dinucleotide (FAD), respectively...
  34. ncbi Refined structures of oxidized flavodoxin from Anacystis nidulans
    C L Drennan
    Department of Biological Chemistry and Biophysics Research Division, University of Michigan, 930 N University Ave, Ann Arbor, MI 48109, USA
    J Mol Biol 294:711-24. 1999
    ..The structures reported here provide the starting point for structure-function studies of the reduced states and of mutants, described in the accompanying paper...
  35. doi Relation between riboflavin, flavin mononucleotide and flavin adenine dinucleotide concentrations in plasma and red cells in patients with critical illness
    Aikaterini T Vasilaki
    University Department of Surgery, University of Glasgow Faculty of Medicine, Royal Infirmary, Glasgow G312ER, Scotland, United Kingdom
    Clin Chim Acta 411:1750-5. 2010
    ..The aim of the present study was to examine the longitudinal interrelationships between riboflavin, flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) in plasma and red cells in patients with critical illness.
  36. ncbi The interaction of NADPH-P450 reductase with P450: an electrochemical study of the role of the flavin mononucleotide-binding domain
    R W Estabrook
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75235 9038, USA
    Arch Biochem Biophys 333:308-15. 1996
    ..It is speculated that a domain composed of acidic amino acids, located near the flavin mononucleotide-binding region of the flavoproteins, may serve as this site...
  37. pmc Flavin mononucleotide-binding flavoprotein family in the domain Archaea
    Yan Huai R Ding
    Department of Biochemistry and Molecular Biology, Eberly College of Science, The Pennsylvania State University, University Park, Pennsylvania 16802 4500, USA
    J Bacteriol 186:90-7. 2004
    ..A search of the databases suggests that AfpA is the prototype of a previously unrecognized flavoprotein family unique to the domain Archaea for which the name archaeoflavoprotein is proposed...
  38. ncbi Synthesis and redox behavior of flavin mononucleotide-functionalized single-walled carbon nanotubes
    Sang Yong Ju
    Nanomaterials Optoelectronics Laboratory, Polymer Program, Department of Chemistry, Institute of Materials Science, University of Connecticut, Storrs, Connecticut 06269 3136, USA
    J Am Chem Soc 130:655-64. 2008
    In this contribution, we describe the synthesis and covalent attachment of an analogue of the flavin mononucleotide (FMN) cofactor onto carboxylic functionalities of single-walled carbon nanotubes (SWNTs)...
  39. doi Selection of carbon nanotubes with specific chiralities using helical assemblies of flavin mononucleotide
    Sang Yong Ju
    Nanomaterials Optoelectronics Laboratory NOEL, Polymer Program, University of Connecticut, Storrs, Connecticut 06269 3136, USA
    Nat Nanotechnol 3:356-62. 2008
    ..Here we show that the flavin mononucleotide, a common redox cofactor, wraps around single-walled carbon nanotubes in a helical pattern that imparts ..
  40. ncbi The 1.59 A resolution crystal structure of TM0096, a flavin mononucleotide binding protein from Thermotoga maritima
    Frances Park
    Structural GenomiX, Inc, San Diego, CA 92121, USA
    Proteins 55:772-4. 2004
  41. ncbi Control of oxidation-reduction potentials in flavodoxin from Clostridium beijerinckii: the role of conformation changes
    M L Ludwig
    Department of Biological Chemistry, University of Michigan, Ann Arbor 48109 1055, USA
    Biochemistry 36:1259-80. 1997
    ..0 kcal/mol) and to increase the sq/hq potential by 80 mV (-1.7 kcal/mol). A thermodynamic scheme is introduced as a framework for rationalizing the properties of wild-type flavodoxin and the effects of the mutations...
  42. ncbi Crystal structure of the FAD/NADPH-binding domain of rat neuronal nitric-oxide synthase. Comparisons with NADPH-cytochrome P450 oxidoreductase
    J Zhang
    Department of Biochemistry, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Biol Chem 276:37506-13. 2001
    ....
  43. doi Reactions of the flavin mononucleotide in complex I: a combined mechanism describes NADH oxidation coupled to the reduction of APAD+, ferricyanide, or molecular oxygen
    James A Birrell
    Medical Research Council Mitochondrial Biology Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 0XY, UK
    Biochemistry 48:12005-13. 2009
    ..Alternatively, NADH oxidation, by the flavin mononucleotide in complex I, can be coupled to the reduction of hydrophilic electron acceptors, in non-energy-transducing ..
  44. ncbi Mechanism of coenzyme binding to human methionine synthase reductase revealed through the crystal structure of the FNR-like module and isothermal titration calorimetry
    Kirsten R Wolthers
    Faculty of Life Sciences, University of Manchester, Manchester Interdisciplinary Biocentre, 131 Princess Street, Manchester M1 7DN, United Kingdom
    Biochemistry 46:11833-44. 2007
    ..The biological implications of an attenuated mechanism of MS reactivation by MSR on methionine and folate metabolism are discussed...
  45. pmc Structure and properties of recombinant human pyridoxine 5'-phosphate oxidase
    Faik N Musayev
    Department of Medicinal Chemistry, Institute for Structural Biology and Drug Discovery, Virginia Commonwealth University, 800 E Leigh Street, Richmond, VA 23219, USA
    Protein Sci 12:1455-63. 2003
    ..As observed with the E. coli enzyme, the human enzyme binds one molecule of pyridoxal 5'-phosphate tightly on each subunit...
  46. ncbi Roseoflavin is a natural antibacterial compound that binds to FMN riboswitches and regulates gene expression
    Elaine R Lee
    Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA
    RNA Biol 6:187-94. 2009
    ..The Gram-positive bacterium Bacillus subtilis has two representatives of a class of riboswitches that bind flavin mononucleotide (FMN)...
  47. ncbi Role of the conserved phenylalanine 181 of NADPH-cytochrome P450 oxidoreductase in FMN binding and catalytic activity
    M J Paine
    ICRF Molecular Pharmacology Unit, Biomedical Research Centre, University of Dundee, Ninewells Hospital and Medical School, Dundee DD1 9SY, UK
    Biochemistry 40:13439-47. 2001
    ..The results indicate that Phe181 cooperates with Tyr140 and Tyr178 to play a major role in the binding and stability of FMN...
  48. pmc A novel reduced flavin mononucleotide-dependent methanesulfonate sulfonatase encoded by the sulfur-regulated msu operon of Pseudomonas aeruginosa
    M A Kertesz
    Institute of Microbiology, Swiss Federal Institute of Technology, ETH Zentrum, CH 8092 Zurich, Switzerland
    J Bacteriol 181:1464-73. 1999
    ..aeruginosa PAO1 and sequenced. It encoded a 381-amino-acid protein that was related to several reduced flavin mononucleotide (FMNH2)-dependent monooxygenases, and it was the second in an operon of three genes, which we have named ..
  49. ncbi Dissecting the energetics of the apoflavodoxin-FMN complex
    A Lostao
    Departamento de Bioquimica y Biologia Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, 50009 Zaragoza, Spain
    J Biol Chem 275:9518-26. 2000
    ..Interestingly, we find crystallographic evidence of flexibility in one of the loops (57-62) involved in isoalloxazine binding...
  50. pmc NADPH-cytochrome P450 oxidoreductase from the mosquito Anopheles minimus: kinetic studies and the influence of Leu86 and Leu219 on cofactor binding and protein stability
    Songklod Sarapusit
    Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Arch Biochem Biophys 477:53-9. 2008
    ..Analysis of the single mutants indicates that the proline to alanine substitution has no impact, but that both leucine to phenylalanine substitutions are essential for FMN binding and maximum stability of the enzyme...
  51. ncbi Expression, purification, and characterization of recombinant Escherichia coli pyridoxine 5'-phosphate oxidase
    M di Salvo
    Institute for Structural Biology and Drug Discovery, Virginia Commonwealth University, Richmond, Virginia, 23298, USA
    Protein Expr Purif 13:349-56. 1998
    ..Using this value and the spectral properties of the bound FMN it was shown by three different methods that the dimeric enzyme contains two molecules of bound FMN per dimer and not one FMN as previously reported...
  52. ncbi Relaxation kinetics of cytochrome P450 reductase: internal electron transfer is limited by conformational change and regulated by coenzyme binding
    Aldo Gutierrez
    Biological NMR Centre, University of Leicester, Medical Sciences Building, P O Box 138, University Road, Leicester LE1 9HN, UK
    Biochemistry 41:4626-37. 2002
    ..The studies reveal altered redox properties of the FAD in W676H CPR. The data are discussed in the context of previous stopped-flow studies of human CPR and the X-ray crystallographic structure of rat CPR...
  53. ncbi Determination of the redox properties of human NADPH-cytochrome P450 reductase
    A W Munro
    Department of Pure and Applied Chemistry, University of Strathclyde, The Royal College, 204 George Street, Glasgow, G1 1XL, UK
    Biochemistry 40:1956-63. 2001
    ..However, they differ markedly from those of yeast and bacterial CPRs, pointing to an important evolutionary difference in electronic regulation of these enzymes...
  54. pmc Idiosyncratically tuned switching behavior of riboswitch aptamer domains revealed by comparative small-angle X-ray scattering analysis
    Nathan J Baird
    Howard Hughes Medical Institute, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109 1024, USA
    RNA 16:598-609. 2010
    ..domains of four different riboswitches that bind, respectively, thiamine pyrophosphate (TPP), flavin mononucleotide (FMN), lysine, and S-adenosyl methionine (SAM)...
  55. pmc Covalent attachment of flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN) to enzymes: the current state of affairs
    M Mewies
    Department of Biochemistry, University of Leicester, UK
    Protein Sci 7:7-20. 1998
    ..Case studies are presented for a variety of covalent flavoenzymes, from which general findings are beginning to emerge...
  56. pmc Regulation of riboflavin biosynthesis in Bacillus subtilis is affected by the activity of the flavokinase/flavin adenine dinucleotide synthetase encoded by ribC
    M Mack
    Biotechnology Section, Vitamin Division, Hoffmann La Roche Ltd, Basel, Switzerland
    J Bacteriol 180:950-5. 1998
    ..The mutation ribC820 leads to deregulation of riboflavin biosynthesis in B. subtilis, most likely by preventing the accumulation of the effector molecule FMN or FAD...
  57. ncbi Molecular determinants for FMN-binding in Desulfovibrio gigas flavoredoxin
    Manuela Broco
    Instituto de Tecnologia Química e Biológica ITQB, Universidade Nova de Lisboa, Av Republica EAN, 2784 505 Oeiras, Portugal
    FEBS Lett 581:4397-402. 2007
    ..These findings were correlated with the protein structure in order to contribute to a better understanding of the structure-function relationships in flavoredoxin...
  58. pmc The bifunctional flavokinase/flavin adenine dinucleotide synthetase from Streptomyces davawensis produces inactive flavin cofactors and is not involved in resistance to the antibiotic roseoflavin
    Simon Grill
    Institut für Technische Mikrobiologie, Hochschule Mannheim, Windeckstr 110, 68163 Mannheim, Germany
    J Bacteriol 190:1546-53. 2008
    ..3 10(-2) microM(-1) s(-1)). Both RibC enzymes synthesized RoFAD and RoFMN. The functional expression of S. davawensis ribC did not confer roseoflavin resistance to a ribC-defective B. subtilis strain...
  59. ncbi Reporter proteins for in vivo fluorescence without oxygen
    Thomas Drepper
    Institute of Molecular Enzyme Technology, Heinrich Heine University Duesseldorf, Research Center Juelich, Stetternicher Forst, D 52426 Juelich, Germany
    Nat Biotechnol 25:443-5. 2007
    ..Bacillus subtilis and Pseudomonas putida that contain light-oxygen-voltage-sensing domains, we engineered flavin mononucleotide-based fluorescent proteins that can be used as fluorescent reporters in both aerobic and anaerobic ..
  60. ncbi Spectroscopic and kinetic characterization of the bifunctional chorismate synthase from Neurospora crassa: evidence for a common binding site for 5-enolpyruvylshikimate 3-phosphate and NADPH
    K Kitzing
    , , , , Switzerland
    J Biol Chem 276:42658-66. 2001
    ....
  61. ncbi Mutagenic analysis of an invariant aspartate residue in chorismate synthase supports its role as an active site base
    Gernot Rauch
    Institute of Biochemistry, Graz University of Technology, Graz, Austria
    Biochemistry 46:3768-74. 2007
    ....
  62. ncbi A structural model for chorismate synthase from Mycobacterium tuberculosis in complex with coenzyme and substrate
    Claudia Lemelle Fernandes
    Laboratório de Bioinformática, Modelagem e Simulação de Biossistemas LABIO, Faculdade de Informática, PUCRS, Brazil
    Comput Biol Med 37:149-58. 2007
    ..However, novel interactions involving ARG111, GLY113 and SER317 were also observed...
  63. ncbi Crystal structure of human riboflavin kinase reveals a beta barrel fold and a novel active site arch
    Subramanian Karthikeyan
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Structure 11:265-73. 2003
    ..The invariant residues Asn36 and Glu86 are implicated in the catalysis...
  64. ncbi Structures of Mycobacterium tuberculosispyridoxine 5'-phosphate oxidase and its complexes with flavin mononucleotide and pyridoxal 5'-phosphate
    Bichitra K Biswal
    CIHR Group in Protein Structure and Function, Department of Biochemistry, University of Alberta, Edmonton T6G 2H7, Canada
    Acta Crystallogr D Biol Crystallogr 61:1492-9. 2005
    ..The structures of flavin mononucleotide (FMN) and pyridoxal 5'-phosphate (PLP) bound separately to Rv1155 have been determined at 2.2 and 1...
  65. ncbi Entropy changes drive the electron transfer reaction of triplet flavin mononucleotide from aromatic amino acids in cation-organized aqueous media. A laser-induced optoacoustic study
    Luis Crovetto
    Max Planck Institut für Bioanorganische Chemie formerly Strahlenchemie, Mulheim an der Ruhr, Germany
    Photochem Photobiol 82:281-90. 2006
    ....
  66. ncbi Crystal structure of chorismate synthase: a novel FMN-binding protein fold and functional insights
    Hyung Jun Ahn
    Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 151 0742, South Korea
    J Mol Biol 336:903-15. 2004
    ..The isoalloxazine ring of the bound FMN is significantly non-planar. Our structure illuminates the essential functional roles played by the cofactor...
  67. pmc An mRNA structure that controls gene expression by binding FMN
    Wade C Winkler
    Department of Molecular, Cellular, and Developmental Biology, Yale University, P O Box 208103, New Haven, CT 06520 8103, USA
    Proc Natl Acad Sci U S A 99:15908-13. 2002
    ..domain that is found frequently in the 5'-untranslated regions of prokaryotic mRNAs that encode for flavin mononucleotide (FMN) biosynthesis and transport proteins...
  68. pmc Identification of a 14mer RNA that recognizes and binds flavin mononucleotide with high affinity
    Peter C Anderson
    School of Pharmacy, University of Wisconsin, 777 Highland Avenue, Madison, WI 53705, USA
    Nucleic Acids Res 33:6992-9. 2005
    ..Here, we show that a 35mer RNA aptamer for the cofactor flavin mononucleotide (FMN) identified by in vitro evolution can be computationally evolved to a mere 14mer structure containing ..
  69. ncbi Genetic control by metabolite-binding riboswitches
    Wade C Winkler
    Department of Molecular, Cellular, and Developmental Biology, Yale University, Kline Biology Tower 506, 266 Whitney Avenue, New Haven, CT 06520, USA
    Chembiochem 4:1024-32. 2003
  70. ncbi Crystal structure of Schizosaccharomyces pombe riboflavin kinase reveals a novel ATP and riboflavin-binding fold
    Stefanie Bauer
    Max Planck Institut fur Biochemie, Abteilung Strukturforschung, Am Klopferspitz 18a, D 82152, Martinsried, Germany
    J Mol Biol 326:1463-73. 2003
    ..The unusual metal-binding site involves, in addition to the ADP phosphates, only the strictly conserved Thr45. This may explain the preference for zinc observed in vitro...
  71. pmc Purification, characterization, and overexpression of flavin reductase involved in dibenzothiophene desulfurization by Rhodococcus erythropolis D-1
    T Matsubara
    Department of Biotechnology, Tottori University, Tottori 680-8552, Japan
    Appl Environ Microbiol 67:1179-84. 2001
    ..The enzyme catalyzed NADH-dependent reduction of flavin mononucleotide (FMN), and the K(m) values for NADH and FMN were 208 and 10.8 microM, respectively...
  72. ncbi Purification and characterization of NfrA1, a Bacillus subtilis nitro/flavin reductase capable of interacting with the bacterial luciferase
    S Zenno
    Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Japan
    Biosci Biotechnol Biochem 62:1978-87. 1998
    ....
  73. ncbi NAD(P)H-flavin oxidoreductase from the bioluminescent bacterium, Vibrio fischeri ATCC 7744, is a flavoprotein
    S Inouye
    Yokohama Research Center, Chisso Corp, Japan
    FEBS Lett 347:163-8. 1994
    ..The purified enzyme had a typical flavoprotein absorption spectrum and flavin mononucleotide (FMN) was identified as a prosthetic group, non-covalently bound in a molar ratio of 1:1...
  74. ncbi Binding of the oxidized, reduced, and radical flavin species to chorismate synthase. An investigation by spectrophotometry, fluorimetry, and electron paramagnetic resonance and electron nuclear double resonance spectroscopy
    P Macheroux
    Nitrogen Fixation Laboratory, John Innes Centre, Norwich Research Park, UK
    Biochemistry 35:1643-52. 1996
    ....
  75. pmc Coenzyme recognition and gene regulation by a flavin mononucleotide riboswitch
    Alexander Serganov
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA
    Nature 458:233-7. 2009
    The biosynthesis of several protein cofactors is subject to feedback regulation by riboswitches. Flavin mononucleotide (FMN)-specific riboswitches, also known as RFN elements, direct expression of bacterial genes involved in the ..
  76. ncbi Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase
    D S Bredt
    Department of Neuroscience, Johns Hopkins Medical Institutions, Baltimore, Maryland 21205
    Nature 351:714-8. 1991
    ..Cloning of a complementary DNA for brain nitric oxide synthase reveals recognition sites for NADPH, FAD, flavin mononucleotide and calmodulin as well as phosphorylation sites, indicating that the synthase is regulated by many ..
  77. ncbi Obligatory intermolecular electron-transfer from FAD to FMN in dimeric P450BM-3
    Tatsuya Kitazume
    Department of Biochemistry, The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas 75390 9038, USA
    Biochemistry 46:11892-901. 2007
    ..Analysis of our results combined with other analyses in the literature suggests that the heme domain of either monomer may accept electrons from the reduced FMN domain...
  78. ncbi Molecular dissection of human methionine synthase reductase: determination of the flavin redox potentials in full-length enzyme and isolated flavin-binding domains
    Kirsten R Wolthers
    Department of Biochemistry, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom
    Biochemistry 42:3911-20. 2003
    ..The redox properties of MSR are discussed in relation to other members of the diflavin oxidoreductase family and the mechanism of electron transfer...
  79. ncbi Structure--function studies on the iron-sulfur flavoenzyme glutamate synthase: an unexpectedly complex self-regulated enzyme
    Maria A Vanoni
    Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita degli Studi di Milano, Via Celoria 26, 20131 Milan, Italy
    Arch Biochem Biophys 433:193-211. 2005
    ..polypeptides of other GltS forms, and involves: (i) an Ntn-type amidotransferase domain and (ii) a flavin mononucleotide-containing (beta/alpha)(8) barrel synthase domain connected by (iii) a approximately 30 A-long ..
  80. ncbi Determination of the redox potentials and electron transfer properties of the FAD- and FMN-binding domains of the human oxidoreductase NR1
    Robert D Finn
    Biomedical Research Centre, University of Dundee, Ninewells Hospital and Medical School, Dundee, UK
    Eur J Biochem 270:1164-75. 2003
    ..Despite overall structural resemblance of NR1 and CPR, our studies reveal thermodynamic similarities but major kinetic differences in the electron transfer reactions catalysed by the flavin-binding domains...
  81. ncbi Chromophore exchange in the LOV2 domain of the plant photoreceptor phototropin1 from oat
    Harald Dürr
    Department of Biology I, Botany, Menzingerstrasse 67, D 80638 München, Germany
    Biochemistry 44:3050-5. 2005
    ..for phototropins are two LOV domains which, when expressed in heterologous systems, each carry a single flavin mononucleotide (FMN) chromophore...
  82. ncbi Dihydroorotate dehydrogenase B of Enterococcus faecalis. Characterization and insights into chemical mechanism
    J Marcinkeviciene
    Department of Chemical Enzymology and Antimicrobial Group, DuPont Pharmaceuticals, Wilmington, Delaware 19880 0400, USA
    Biochemistry 38:13129-37. 1999
    ..8-6.3 and reflects either deprotonation of the reduced flavin or binding of orotate; this step is followed by hydride transfer to C6 and general acid-assisted protonation (pK of 9.1 +/- 0.2) at C5 of the product...
  83. ncbi Structural analysis of interactions for complex formation between Ferredoxin-NADP+ reductase and its protein partners
    Tomas Mayoral
    Grupo de Cristalografia Macromolecular y Biologia Estructural, Instituto Quimica Fisica Rocasolano, C S I C Serrano 119, 28006 Madrid, Spain
    Proteins 59:592-602. 2005
    ....
  84. pmc Riboflavin biosynthesis is associated with assimilatory ferric reduction and iron acquisition by Campylobacter jejuni
    Rachel A Crossley
    Institute of Food Research, Office E410, Norwich Research Park, Colney Lane, Norwich NR4 7UA, United Kingdom
    Appl Environ Microbiol 73:7819-25. 2007
    ..precursor diacetyl but not in the presence of the downstream products flavin adenine dinucleotide and flavin mononucleotide. Riboflavin uptake was unaffected in the ribB mutant under iron-limited conditions but was lower in both ..
  85. ncbi Versatile regulation of neuronal nitric oxide synthase by specific regions of its C-terminal tail
    Mauro Tiso
    Department of Pathobiology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA
    Biochemistry 46:14418-28. 2007
    ..Our results reveal that the CT exerts both multifaceted and regiospecific effects on catalytic activities and related behaviors, and thus provide new insights into mechanisms that regulate nNOS catalysis...
  86. ncbi Cloning and characterization of a novel human dual flavin reductase
    M J Paine
    Imperial Cancer Research Fund Molecular Pharmacology Unit, Biomedical Research Centre, University of Dundee, Ninewells Hospital and Medical School, Dundee DD1 9SY, United Kingdom
    J Biol Chem 275:1471-8. 2000
    ....
  87. ncbi Mechanistic studies on the intramolecular one-electron transfer between the two flavins in the human neuronal nitric-oxide synthase and inducible nitric-oxide synthase flavin domains
    Zhi Wen Guan
    Department of Life Science, Faculty of Science, Himeji Institute of Technology, Harima Science Garden City, Hyogo, 678 1279 Japan
    J Biol Chem 278:30859-68. 2003
    ..The data indicate a more favorable formation of the active intermediate FMNH2 in the iNOS flavin domain...
  88. ncbi Interflavin electron transfer in human cytochrome P450 reductase is enhanced by coenzyme binding. Relaxation kinetic studies with coenzyme analogues
    Aldo Gutierrez
    Department of Biochemistry and Biological NMR Centre, University of Leicester, UK
    Eur J Biochem 270:2612-21. 2003
    ..e. enhanced at low coenzyme concentration) observed in stopped-flow studies. The data are discussed in the context of previous kinetic studies and of the crystallographic structure of rat CPR...
  89. ncbi Thermodynamic basis of electron transfer in dihydroorotate dehydrogenase B from Lactococcus lactis: analysis by potentiometry, EPR spectroscopy, and ENDOR spectroscopy
    Al Walid A Mohsen
    Department of Biochemistry, University of Leicester, UK
    Biochemistry 43:6498-510. 2004
    ..From potentiometric studies, the reduction of the 2Fe-2S center and the reduction of the FMN occur concomitantly. The study provides a detailed thermodynamic framework for electron transfer in this complex iron-sulfur flavoprotein...
  90. ncbi X-ray crystal structure of the Desulfovibrio vulgaris (Hildenborough) apoflavodoxin-riboflavin complex
    M A Walsh
    Department of Chemistry, University College, Galway, Ireland
    Eur J Biochem 258:362-71. 1998
    ....
  91. ncbi Structure of the flavocoenzyme of two homologous amine oxidases: monomeric sarcosine oxidase and N-methyltryptophan oxidase
    M A Wagner
    Department of Biochemistry, MCP Hahnemann School of Medicine, Philadelphia, Pennsylvania 19129, USA
    Biochemistry 38:5588-95. 1999
    ..There is only one conserved cysteine found among these enzymes, suggesting that Cys308 is the covalent flavin attachment site in MTOX...
  92. ncbi An appraisal of multiple NADPH binding-site models proposed for cytochrome P450 reductase, NO synthase, and related diflavin reductase systems
    Simon Daff
    School of Chemistry, University of Edinburgh, West Mains Road, Edinburgh EH9 3JJ, UK
    Biochemistry 43:3929-32. 2004
    ..A detailed reanalysis shows that the data are in fact consistent with a simple single-binding-site model in which reversible hydride transfer causes the observed effect. Data for the related systems are also discussed...
  93. ncbi Kinetic and thermodynamic characterization of the common polymorphic variants of human methionine synthase reductase
    Horatiu Olteanu
    Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    Biochemistry 43:1988-97. 2004
    ..It is likely that differences in their relative affinities for the redox partner, methionine synthase, underlie the differences in the relative efficiencies of reductive activation exhibited by the variants...
  94. ncbi Gene cloning, purification, and characterization of NfsB, a minor oxygen-insensitive nitroreductase from Escherichia coli, similar in biochemical properties to FRase I, the major flavin reductase in Vibrio fischeri
    S Zenno
    Department of Biophysics and Biochemistry, Graduate School of Sciences, and Biotechnology Research Center, The University of Tokyo
    J Biochem 120:736-44. 1996
    ..The NfsB catalysis obeys the ping pong Bi-Bi mechanism. The Km value for NADH varied depending on the second substrate used...
  95. ncbi Biochemical analysis of mutations in P450 oxidoreductase
    A V Pandey
    Theodor Kocher Institute, University of Bern, Freiestrasse 1, CH 3010 Bern, Switzerland
    Biochem Soc Trans 34:1186-91. 2006
    ..Understanding the genetic and biochemical basis of POR-mediated drug metabolism will provide valuable information about possible differences in P450-mediated reactions among the individuals carrying a variant or polymorphic form of POR...
  96. ncbi Potentiometric and further kinetic characterization of the flavin-binding domain of Saccharomyces cerevisiae flavocytochrome b2. Inhibition by anions binding in the active site
    Narimantas Cenas
    Institute of Biochemistry, Mokslininku 12, Vilnius 2600, Lithuania
    Biochemistry 46:4661-70. 2007
    ..According to literature data, it must also be present in other members of the family of L-2-hydroxy acid-oxidizing enzymes...
  97. ncbi Electrochromatographic retention studies on a flavin-binding RNA aptamer sorbent
    Stacey L Clark
    Department of Chemistry, 153 Gilbert Hall, Oregon State University, Corvallis, Oregon 97331 4003, USA
    Anal Chem 75:5692-6. 2003
    ..In this study, an RNA aptamer isolated and amplified on the basis of its affinity for flavin mononucleotide (FMN) was covalently bound to the inner walls of fused-silica capillaries...
  98. ncbi Clinical, structural and functional implications of mutations and polymorphisms in human NADPH P450 oxidoreductase
    Christa E Flück
    Department of Pediatrics Endocrinology, University Children s Hospital, Freiburgstrasse 15, 3010 Bern, Switzerland
    Fundam Clin Pharmacol 21:399-410. 2007
    ..POR is a flavoprotein that contains both flavin mononucleotide and flavin adenine dinucleotide as cofactors and uses NADPH as the source of electrons...
  99. ncbi Proton-coupled electron transfer of flavodoxin immobilized on nanostructured tin dioxide electrodes: thermodynamics versus kinetics control of protein redox function
    Yeni Astuti
    Department of Chemistry, Imperial College London, South Kensington, London SW7 2AZ, UK
    J Am Chem Soc 126:8001-9. 2004
    ..spectroelectrochemistry to the quinone/semiquinone and semiquinone/hydroquinone couples of the protein's flavin mononucleotide (FMN) redox cofactor...
  100. ncbi A new approach to the study of protein-protein interaction by FTIR: complex formation between cytochrome P450BM-3 heme domain and FMN reductase domain
    Andrei Kariakin
    Max Delbruck Center for Molecular Medicine, Protein Dynamics Laboratory, 13125 Berlin, Germany
    Biochemistry 41:13514-25. 2002
    ....
  101. ncbi Crystal structure of NAD(P)H:flavin oxidoreductase from Escherichia coli
    M Ingelman
    Department of Molecular Biology, Swedish University of Agricultural Sciences, Biomedical Center, Uppsala
    Biochemistry 38:7040-9. 1999
    ..Interactions for flavin substrates are instead provided by a hydrophobic isoalloxazine binding site that also contains a serine and a threonine, which form hydrogen bonds to the isoalloxazine of bound riboflavin in a substrate complex...

Research Grants105 found, 100 shown here

  1. STRUCTURAL STUDIES ON NITRIC OXIDE SYNTHASE
    Thomas Poulos; Fiscal Year: 2001
    ..Crystals of the eNOS heme domain diffract to 2.5A and smaller crystals of the nNOS and iNOS heme domains have been obtained. Our initial goal will be to solve the crystal structure of the eNOS heme domain. ..
  2. Flavoenzymes in Pyrimidine Metabolism
    Bruce A Palfey; Fiscal Year: 2011
    ..Our studies of the rates of the chemical reactions will identify important parts of the proteins, how they move during reactions, how they speed the synthesis of products, and how these reactions might be blocked. ..
  3. Pharmacogenomics of Human P450 Oxidoreductase
    Walter L Miller; Fiscal Year: 2010
    ..amino-acid, 78 kDa protein that contains two flavins, a flavin adenine dinucleotide moiety (FAD), and a flavin mononucleotide (FMN). There is substantial genetic variation in the hepatic P450 enzymes...
  4. Pharmacogenomics of Human P450 Oxidoreductase
    WALTER MILLER; Fiscal Year: 2009
    ..amino-acid, 78 kDa protein that contains two flavins, a flavin adenine dinucleotide moiety (FAD), and a flavin mononucleotide (FMN). There is substantial genetic variation in the hepatic P450 enzymes...
  5. Structural Basis for Isoprenoid Biosynthesis
    JOSEPH NOEL; Fiscal Year: 2006
    ..This type 2 IPP isomerase, unlike the well-studied type 1 enzymes, depends on flavin mononucleotide and NADPH for activity...
  6. Nitrite Mediated Cardioprotection
    DAVID JOSEPH LEFER; Fiscal Year: 2010
    ..The synthesis of NO is critically influenced by various cofactors such as tetrahydrobiopterin, flavin mononucleotide and flavin adenine dinucleotide, the presence of reduced thiols, and the endogenous NOS inhibitor ..
  7. Mechanistic Studies of Type II IPP Isomerase
    Steven Rothman; Fiscal Year: 2006
    A recent report describes the cloning and preliminary characterization of an unusual flavin mononucleotide (FMN) dependent enzyme, the type II isopentenyl diphosphate (IPP) isomerase...
  8. COFACTOR ROLE IN BETA-SHEET PROTEIN FOLDING
    Pernilla Wittung Stafshede; Fiscal Year: 2005
    ..a copper-ion cofactor, flavodoxin, a protein with an a/b doubly-wound topology coordinating an organic flavin mononucleotide (FMN), and cytochrome f, a b-sheet protein covalently linked to a heme...
  9. Energetics, kinetics and mechanism in gene regulatory processes
    DONALD CROTHERS; Fiscal Year: 2009
    ..Riboswitches that respond to flavin mononucleotide (FMN), adenine, and glycine are the current targets of study...
  10. Energetics, kinetics and mechanism in gene regulatory processes
    DONALD CROTHERS; Fiscal Year: 2007
    ..Riboswitches that respond to flavin mononucleotide (FMN), adenine, and glycine are the current targets of study...
  11. Mechanisms of Electron Transfer in Nitric Oxide Synthases: the Output State in Ni
    Changjian Feng; Fiscal Year: 2007
    ..of NO synthesis in endothelial and neuronal NOS (eNOS and nNOS) requires a conformational change of the flavin mononucleotide (FMN) domain from its original electron- accepting (input) state to a new electron-donating (output) state...
  12. Mechanisms of Electron Transfer in Nitric Oxide Synthases: the Output State in Ni
    Changjian Feng; Fiscal Year: 2007
    ..of NO synthesis in endothelial and neuronal NOS (eNOS and nNOS) requires a conformational change of the flavin mononucleotide (FMN) domain from its original electron- accepting (input) state to a new electron-donating (output) state...
  13. Modular Enzymatic Assembly Lines for Antibiotics
    Christopher T Walsh; Fiscal Year: 2010
    ..These include ester backbone linkages in place of amides, the recruitment of transglutaminase homologs as condensation domains that act in trans, formation of C-terminal pyrrolidinedione rings, and reductive chain termination steps. ..
  14. Folate Receptor-Mediated siRNA Delivery to Cancer Cells
    Faqing Huang; Fiscal Year: 2007
    ....
  15. BIOCHEMISTRY OF NITRIC OXIDE SYNTHESIS
    Dennis Stuehr; Fiscal Year: 2005
    ..2) Generate truncated NOS to analyze domain interaction by crystallography.(3) Evaluate a role for charged surface residues in domain docking,(4) Evaluate three candidate pathways for electron delivery to heme and/or H4B. ..
  16. BIOCHEMISTRY OF MACROPHAGE NITRIC OXIDE SYNTHESIS
    Dennis Stuehr; Fiscal Year: 2000
    ..Together, this will provide a comprehensive picture regarding how dimer formation activates iNOS, and how it can be controlled. ..
  17. CONTROL MECHANISMS OF THE NITRIC OXIDE SYNTHASES
    Dennis J Stuehr; Fiscal Year: 2010
    ..By clarifying how nitric oxide production is regulated at the enzyme level, our work may help to develop treatments for human diseases that involve making too much or too little nitric oxide. ..
  18. Structural Studies on Cytochromes P450 and Related Proteins
    THOMAS LEWIS POULOS; Fiscal Year: 2010
    ..Especially important is the role played by P450s in drug metabolism, cancer, and the biosynthesis of critical steroid intermediates and antibiotics. ..
  19. Flavoenzymes in Pyrimidine Metabolism
    Bruce A Palfey; Fiscal Year: 2010
    ..Our studies of the rates of the chemical reactions will identify important parts of the proteins, how they move during reactions, how they speed the synthesis of products, and how these reactions might be blocked. ..
  20. Flavoenzymes in Pyrimidine Metabolism
    Bruce A Palfey; Fiscal Year: 2010
    ..We will determine the substrate specificities of selected model DUSs and probe the interactions of the protein and tRNA by chemical and biophysical means. Project Description Page 6 ..
  21. HEME ENZYME ENGINEERING
    Thomas Poulos; Fiscal Year: 2004
    ..The primary methods to employed are protein engineering coupled with crystallography and other biophysical approaches such as EPR spectroscopy and flash photolysis methods for following rapid reactions. ..
  22. BIOCHEMISTRY OF NITRIC OXIDE SYNTHESIS
    Dennis J Stuehr; Fiscal Year: 2010
    ..Relevance: By clarifying how nitric oxide production occurs and is regulated, our work may help to develop treatments for human diseases that involve making too much or too little nitric oxide. ..
  23. THERMODYNAMICS OF BIOCHEMICAL SYSTEMS
    ROBERT ALBERTY; Fiscal Year: 2005
    ..This quantitative treatment of the literature data on biochemical reactions will uncover errors because deltafG'0 values can be determined by use of two or more biochemical reactions, which should give the same answer. ..
  24. THERMODYNAMICS OF BIOCHEMICAL SYSTEMS
    ROBERT ALBERTY; Fiscal Year: 2001
    ..Another objective is to use new concepts in biochemical thermodynamics to investigate coupling of reactions by enzymes, missing reactions in systems like glycolysis, muscle, and chemosmotic theory. ..
  25. DNA-based Arrays of Cross-reactive Molecular Sensors
    Milan Stojanovic; Fiscal Year: 2004
    ..In the next phase, DNA-based cross-reactive sensors for oligosaccharides will be incorporated into arrays to expand the panel of metabolites that could be profiled in a single-step assay. ..
  26. Protein Expression, Nitration and Gene Changes in ALS
    Dennis Stuehr; Fiscal Year: 2002
    ..These include markers for disease progression, treatment efficacy, and high-throughput drug discovery. Our work with non-neuronal tissue may identify markers that provide far more-convenient monitoring. ..
  27. CONTROL MECHANISMS OF THE NITRIC OXIDE SYNTHASES
    Dennis Stuehr; Fiscal Year: 2009
    ..By clarifying how nitric oxide production is regulated at the enzyme level, our work may help to develop treatments for human diseases that involve making too much or too little nitric oxide. ..