adenylyl imidodiphosphate

Summary

Summary: 5'-Adenylic acid, monoanhydride with imidodiphosphoric acid. An analog of ATP, in which the oxygen atom bridging the beta to the gamma phosphate is replaced by a nitrogen atom. It is a potent competitive inhibitor of soluble and membrane-bound mitochondrial ATPase and also inhibits ATP-dependent reactions of oxidative phosphorylation.

Top Publications

  1. ncbi Crystal structure of an N-terminal fragment of the DNA gyrase B protein
    D B Wigley
    Department of Chemistry, York University, Heslington, UK
    Nature 351:624-9. 1991
  2. pmc Flexibility in the ABC transporter MsbA: Alternating access with a twist
    Andrew Ward
    Departments of Cell Biology and Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, CB 105, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 104:19005-10. 2007
  3. ncbi Structure of the multidrug ABC transporter Sav1866 from Staphylococcus aureus in complex with AMP-PNP
    Roger J P Dawson
    Institute of Molecular Biology and Biophysics, ETH Zurich HPK D14 3, 8093 Zurich, Switzerland
    FEBS Lett 581:935-8. 2007
  4. ncbi Differential and simultaneous adenosine di- and triphosphate binding by MutS
    Keith P Bjornson
    Department of Biochemistry and Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 278:18557-62. 2003
  5. pmc The alternating ATPase domains of MutS control DNA mismatch repair
    Meindert H Lamers
    Division of Molecular Carcinogenesis, Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    EMBO J 22:746-56. 2003
  6. ncbi Differential interactions of nucleotides at the two nucleotide binding domains of the cystic fibrosis transmembrane conductance regulator
    L Aleksandrov
    S. C. Johnson Medical Research Center, Mayo Clinic Scottsdale, Scottsdale, Arizona 85259, USA
    J Biol Chem 276:12918-23. 2001
  7. ncbi Nucleotide-dependent single- to double-headed binding of kinesin
    K Kawaguchi
    Department of Physics, School of Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan
    Science 291:667-9. 2001
  8. ncbi Drosophila kinesin motor domain extending to amino acid position 392 is dimeric when expressed in Escherichia coli
    T G Huang
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213
    J Biol Chem 269:16502-7. 1994
  9. ncbi A human topoisomerase II alpha heterodimer with only one ATP binding site can go through successive catalytic cycles
    Camilla Skouboe
    Department of Molecular Biology, University of Aarhus, C F Møllers Alle, Building 130, Arhus C 8000, Denmark
    J Biol Chem 278:5768-74. 2003
  10. pmc Mutation of Walker-A lysine 464 in cystic fibrosis transmembrane conductance regulator reveals functional interaction between its nucleotide-binding domains
    Allan C Powe
    Department of Physiology and Dalton Cardiovascular Research Center, University of Missouri Columbia, 134 Research Park Drive, Columbia, MO 65211, USA
    J Physiol 539:333-46. 2002

Research Grants

  1. High Resolution Labels for EM
    James F Hainfeld; Fiscal Year: 2010
  2. KINETIC STUDIES OF ENZYME MECHANISMS
    WILLIAM CLELAND; Fiscal Year: 2004
  3. MYOFIBRILLAR STRUCTURE OF STRIATED MUSCLE
    MICHAEL KAY REEDY; Fiscal Year: 2010
  4. MYOFIBRILLAR STRUCTURE OF STRIATED MUSCLE
    Michael Reedy; Fiscal Year: 2007
  5. REGULATION OF THE INSULIN RECEPTOR KINASE
    Jeffrey Pessin; Fiscal Year: 1991
  6. DYNAMIC EVENTS IN MYOSIN DURING CONTRACTION OF MUSCLE
    Emil Reisler; Fiscal Year: 1990
  7. MYOFIBRILLAR STRUCTURE OF STRIATED MUSCLE
    Michael Reedy; Fiscal Year: 2005
  8. MYOFIBRILLAR STRUCTURE OF STRIATED MUSCLE
    Michael Reedy; Fiscal Year: 2006
  9. Pancreatic zymogen activation in a reconstituted system
    Edwin Thrower; Fiscal Year: 2006
  10. Function and regulation of cardiac Na transporters
    Donald Hilgemann; Fiscal Year: 2007

Detail Information

Publications180 found, 100 shown here

  1. ncbi Crystal structure of an N-terminal fragment of the DNA gyrase B protein
    D B Wigley
    Department of Chemistry, York University, Heslington, UK
    Nature 351:624-9. 1991
    ..The C-terminal domains form the sides of a 20 A hole through the protein dimer which may play a role in DNA strand passage during the supercoiling reaction...
  2. pmc Flexibility in the ABC transporter MsbA: Alternating access with a twist
    Andrew Ward
    Departments of Cell Biology and Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, CB 105, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 104:19005-10. 2007
    ..Altogether, the conformational changes between these structures suggest that large ranges of motion may be required for substrate transport...
  3. ncbi Structure of the multidrug ABC transporter Sav1866 from Staphylococcus aureus in complex with AMP-PNP
    Roger J P Dawson
    Institute of Molecular Biology and Biophysics, ETH Zurich HPK D14 3, 8093 Zurich, Switzerland
    FEBS Lett 581:935-8. 2007
    ..The results confirm that the ATP-bound state of multidrug ABC transporters is coupled to an outward-facing conformation of the transmembrane domains...
  4. ncbi Differential and simultaneous adenosine di- and triphosphate binding by MutS
    Keith P Bjornson
    Department of Biochemistry and Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 278:18557-62. 2003
    ..These findings suggest that the protein exists primarily as the ATP.MutS.ADP ternary complex in solution and that this may be the form of the protein that is involved in DNA encounters in vivo...
  5. pmc The alternating ATPase domains of MutS control DNA mismatch repair
    Meindert H Lamers
    Division of Molecular Carcinogenesis, Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    EMBO J 22:746-56. 2003
    ..We propose that asymmetry of the ATPase domains is an essential feature of mismatch repair that controls the timing of the different steps in the repair cascade...
  6. ncbi Differential interactions of nucleotides at the two nucleotide binding domains of the cystic fibrosis transmembrane conductance regulator
    L Aleksandrov
    S. C. Johnson Medical Research Center, Mayo Clinic Scottsdale, Scottsdale, Arizona 85259, USA
    J Biol Chem 276:12918-23. 2001
    ..This argues strongly that AMP-PNP does not inhibit ATP hydrolysis at NBD2 and thereby questions the relevance of hydrolysis at that domain to channel closing...
  7. ncbi Nucleotide-dependent single- to double-headed binding of kinesin
    K Kawaguchi
    Department of Physics, School of Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan
    Science 291:667-9. 2001
    ..Thus, kinesin binds through two heads in the former and one head in the latter two states, which supports a major prediction of the hand-over-hand model...
  8. ncbi Drosophila kinesin motor domain extending to amino acid position 392 is dimeric when expressed in Escherichia coli
    T G Huang
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213
    J Biol Chem 269:16502-7. 1994
    ..Electron microscopy indicates that both DKH340 monomers and DKH392 dimers decorate microtubules with a periodicity of 8 nm...
  9. ncbi A human topoisomerase II alpha heterodimer with only one ATP binding site can go through successive catalytic cycles
    Camilla Skouboe
    Department of Molecular Biology, University of Aarhus, C F Møllers Alle, Building 130, Arhus C 8000, Denmark
    J Biol Chem 278:5768-74. 2003
    ..The results can be explained in a model, where efficient catalysis requires an extensive communication between the second ATP and the DNA segment to be cleaved...
  10. pmc Mutation of Walker-A lysine 464 in cystic fibrosis transmembrane conductance regulator reveals functional interaction between its nucleotide-binding domains
    Allan C Powe
    Department of Physiology and Dalton Cardiovascular Research Center, University of Missouri Columbia, 134 Research Park Drive, Columbia, MO 65211, USA
    J Physiol 539:333-46. 2002
    ..Delay of closing in wild type by adenylyl imidodiphosphate (AMP-PNP), a non-hydrolysable ATP analogue, is markedly diminished in K464A mutants due to reduction in ..
  11. ncbi Release of isolated single kinesin molecules from microtubules
    Y Vugmeyster
    Department of Biochemistry and The Center for Complex Systems, Brandeis University, Waltham, Massachusetts 02254, USA
    Biochemistry 37:747-57. 1998
    ..The inhibitor adenylyl imidodiphosphate (AMP-PNP) induces stochastic pauses in the movement of bead-labeled enzyme molecules in 1 mM ATP...
  12. ncbi Nucleotide-dependent domain movement in the ATPase domain of a human type IIA DNA topoisomerase
    Hua Wei
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    J Biol Chem 280:37041-7. 2005
    ..Comparison of these structures revealed rigid-body movement of the structural modules within the ATPase domain, suggestive of the motions of a molecular gate...
  13. ncbi Structure of the AAA ATPase p97
    X Zhang
    Molecular Structure and Function Laboratory, Imperial Cancer Research Fund, London SW7 2AY, United Kingdom
    Mol Cell 6:1473-84. 2000
    ..A comparison with NSF D2 (ATP complex) reveals possible conformational changes induced by ATP hydrolysis. Given the D1 and D2 packing arrangement, we propose a ratchet mechanism for p97 during its ATP hydrolysis cycle...
  14. ncbi Structural insight into nucleotide recognition in tau-protein kinase I/glycogen synthase kinase 3 beta
    Masaaki Aoki
    Mitsubishi Kagaku Institute of Life Sciences MITILS, 11 Minamiohya, Machida, Tokyo 194 8511, Japan
    Acta Crystallogr D Biol Crystallogr 60:439-46. 2004
    ....
  15. ncbi The high affinity ATP binding site modulates the SecA-precursor interaction
    F van Voorst
    CBLE, Institute of Biomembranes, Department of Biochemistry of Membranes, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands
    FEBS Lett 486:57-62. 2000
    ..Examining SecA ATP binding site mutants demonstrated that ATP hydrolysis at the N-terminal high affinity binding site is responsible for the changed interaction with the preprotein...
  16. ncbi A kinesin-like motor inhibits microtubule dynamic instability
    Henrik Bringmann
    Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, Meyerhofstrabetae 1, 69117 Heidelberg, Germany
    Science 303:1519-22. 2004
    ..The integration of the two properties, motility and inhibition of microtubule dynamics, in one molecule emphasizes the versatile properties of kinesin family members...
  17. ncbi pH-controlled quaternary states of hexameric DnaB helicase
    L E Donate
    Campus de Cantoblanco, Centro Nacional de Biotecnologia CSIC, Madrid, 28049, Spain
    J Mol Biol 303:383-93. 2000
    ..Structural consequences of this quaternary polymorphism, as well as its functional implications for helicase activity, are discussed...
  18. ncbi Nucleotide exchange from the high-affinity ATP-binding site in SecA is the rate-limiting step in the ATPase cycle of the soluble enzyme and occurs through a specialized conformational state
    John J Fak
    Department of Biological Sciences, 702A Fairchild Center, MC2434, Columbia University, New York, New York 10027, USA
    Biochemistry 43:7307-27. 2004
    ....
  19. ncbi KIF1A alternately uses two loops to bind microtubules
    Ryo Nitta
    Department of Cell Biology and Anatomy, University of Tokyo, Graduate School of Medicine, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Science 305:678-83. 2004
    ..We report crystal structures of monomeric kinesin KIF1A with three transition-state analogs: adenylyl imidodiphosphate (AMP-PNP), adenosine diphosphate (ADP)-vanadate, and ADP-AlFx (aluminofluoride complexes)...
  20. pmc Structure of the photolyase-like domain of cryptochrome 1 from Arabidopsis thaliana
    Chad A Brautigam
    Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9050, USA
    Proc Natl Acad Sci U S A 101:12142-7. 2004
    ..ATP, and we observe a single molecule of an ATP analog bound in the aforementioned surface cavity, near the bound FAD cofactor. The structure has implications for the signaling mechanism of CRY blue-light photoreceptors...
  21. ncbi Inter-domain motions of the N-domain of the KdpFABC complex, a P-type ATPase, are not driven by ATP-induced conformational changes
    Melina Haupt
    Institut fur Organische Chemie und Biochemie, Technische Universitat Munchen, Lichtenbergstr 4, 85747 Garching, Germany
    J Mol Biol 342:1547-58. 2004
    ..No evidence of significant conformational changes was observed within the N-domain upon nucleotide binding, thus ruling out a role for ATP-induced conformational changes in the reaction cycle...
  22. ncbi Function-dependent conformational changes of the ABCG2 multidrug transporter modify its interaction with a monoclonal antibody on the cell surface
    Csilla Ozvegy-Laczka
    National Medical Center, Institute of Haematology and Immunology, Membrane Research Group and Hungarian Academy of Sciences, 1113 Budapest, Hungary
    J Biol Chem 280:4219-27. 2005
    ....
  23. pmc Modulation of kinesin binding by the C-termini of tubulin
    Georgios Skiniotis
    European Molecular Biology Laboratory, Meyerhofstrasse 1, Heidelberg, Germany
    EMBO J 23:989-99. 2004
    ..Overall, our study reveals that the tubulin C-termini define the stability of the MT-kinesin complex in a nucleotide-dependent manner, and highlights the involvement of tubulin in the regulation of weak and strong kinesin binding states...
  24. ncbi ATP analogue binding to the A subunit induces conformational changes in the E subunit that involves a disulfide bond formation in plant V-ATPase
    Y Kawamura
    Cryobiosystem Research Center, Iwate University, Iwate, Japan
    Eur J Biochem 268:2801-9. 2001
    ..This is the first demonstration of interaction between the A and E subunits in the substrate-binding state of a plant V-ATPase...
  25. ncbi Temperature and ligand dependence of conformation and helical order in myosin filaments
    S Xu
    Laboratory of Muscle Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Biochemistry 42:390-401. 2003
    ..Low temperatures favor one disordered conformation, while high temperatures favor the ordered (closed) conformation together with a second disordered conformation...
  26. ncbi Dissociation of the single-ring chaperonin GroEL by high hydrostatic pressure
    Markandeswar Panda
    Department of Biochemistry, Mail Code 7760, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, Texas 78229 3900, USA
    Biochemistry 41:12843-9. 2002
    ..The results presented in this investigation demonstrate that the presence of a second ring in the GroEL 14mer is important for its stability in an environment where the functional ligands of the chaperonin are available...
  27. ncbi A mechanism for microtubule depolymerization by KinI kinesins
    Carolyn A Moores
    Department of Cell Biology, CB227, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
    Mol Cell 9:903-9. 2002
    ..Thus, the relatively small class-specific differences within the KinI motor core modulate a fundamentally conserved mode of interaction with microtubules to produce a unique depolymerizing activity...
  28. ncbi The nucleotide-binding domains of P-glycoprotein. Functional symmetry in the isolated domain demonstrated by N-ethylmaleimide labelling
    Georgina Berridge
    Nuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford, UK
    Eur J Biochem 270:1483-92. 2003
    ..Any observed functional asymmetry in the intact transporter presumably reflects different rates of hydrolysis at the two NBDs or interdomain communications...
  29. ncbi PurT-encoded glycinamide ribonucleotide transformylase. Accommodation of adenosine nucleotide analogs within the active site
    James B Thoden
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Biol Chem 277:23898-908. 2002
    ..This sensitivity to nucleotide identity is in sharp contrast to that observed for the "P-loop"-containing enzymes, in which the conformation of the binding motif is virtually unchanged in the presence or absence of nucleotides...
  30. ncbi Regulated cycling of mitochondrial Hsp70 at the protein import channel
    Qinglian Liu
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    Science 300:139-41. 2003
    ..Consistent with the ratchet model of translocation, subsequent hydrolysis of ATP would trap the polypeptide, driving import by preventing its movement back toward the cytosol...
  31. ncbi Thermodynamic characterization of the binding of nucleotides to glycyl-tRNA synthetase
    John David Dignam
    Department of Biochemistry and Molecular Biology, Medical College of Ohio, 3035 Arlington Avenue, Toledo, Ohio 43614 5804, USA
    Biochemistry 42:5333-40. 2003
    ..The results are consistent with a significant conformational transition in glycyl-tRNA synthetase coupled to the binding of GSAd...
  32. ncbi The role of ATP hydrolysis for kinesin processivity
    Christopher M Farrell
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Biol Chem 277:17079-87. 2002
    ..This mechanism optimizes forward movement and processivity by ensuring that one motor domain is tightly bound to the microtubule before the second can detach...
  33. ncbi Crystal structure of monomeric actin in the ATP state. Structural basis of nucleotide-dependent actin dynamics
    Philip Graceffa
    Boston Biomedical Research Institute, Watertown, Massachusetts 02472, USA
    J Biol Chem 278:34172-80. 2003
    ..An analysis of the existing structures of members of the actin superfamily suggests that the cleft is open in the nucleotide-free state...
  34. ncbi Distinguishing inchworm and hand-over-hand processive kinesin movement by neck rotation measurements
    Wei Hua
    Biophysics and Structural Biology Program, Biochemistry Department, Brandeis University, Waltham, MA 02454 9110, USA
    Science 295:844-8. 2002
    ..In fact, there were no rotations, a finding that is inconsistent with symmetric hand-over-hand movement. An alternative "inchworm" mechanism is consistent with our experimental results...
  35. ncbi Discrimination of ATP, ADP, and AMPPNP by chaperonin GroEL: hexokinase treatment revealed the exclusive role of ATP
    Fumihiro Motojima
    Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Midori ku, Yokohama 226 8503, Japan
    J Biol Chem 278:26648-54. 2003
    ..In the absence of hexokinase, apparent cis folding of rhodanese and malate dehydrogenase was observed in ADP and AMPPNP. Thus, the exclusive role of ATP in generation of the cis ternary complex is now evident...
  36. ncbi Interactions of the Escherichia coli DnaB helicase hexamer with the replication factor the DnaC protein. Effect of nucleotide cofactors and the ssDNA on protein-protein interactions and the topology of the complex
    Roberto Galletto
    Department of Human Biological Chemistry and Genetics, The University of Texas Medical Branch at Galveston, Galveston, TX 77555 1053, USA
    J Mol Biol 329:441-65. 2003
    ..In solution, the DnaC protein-binding site is located on the large 33 kDa domain of the DnaB helicase. The significance of the results in the functioning of the DnaB helicase-DnaC protein complex is discussed...
  37. ncbi ATP binding to the first nucleotide-binding domain of multidrug resistance protein MRP1 increases binding and hydrolysis of ATP and trapping of ADP at the second domain
    Yue Xian Hou
    Mayo Foundation, S C Johnson Medical Research Center, Mayo Clinic Scottsdale, Scottsdale, Arizona 85259, USA
    J Biol Chem 277:5110-9. 2002
    ..The interactions between the two asymmetric NBDs of MRP1 protein may enhance the catalytic efficiency of the MRP1 protein and hence of its ATP-dependent transport of conjugated anions out of cells...
  38. pmc Crystal structures of active fully assembled substrate- and product-bound complexes of UDP-N-acetylmuramic acid:L-alanine ligase (MurC) from Haemophilus influenzae
    Clifford D Mol
    Syrrx Inc, San Diego, California 92121, USA
    J Bacteriol 185:4152-62. 2003
    ....
  39. ncbi Structural analyses of nucleotide binding to an aminoglycoside phosphotransferase
    D L Burk
    Antimicrobial Research Centre and Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada L8N 3Z5
    Biochemistry 40:8756-64. 2001
    ..This alternate binding mode is likely a conserved feature among aminoglycoside kinases and could be exploited for the structure-based drug design of compounds to combat antibiotic resistance...
  40. ncbi Transformation of MutL by ATP binding and hydrolysis: a switch in DNA mismatch repair
    C Ban
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Cell 97:85-97. 1999
    ..Dimerization of the LN40 region is required for and is the rate-limiting step in ATP hydrolysis by MutL. The ATPase activity of MutL is stimulated by DNA and likely acts as a switch to coordinate DNA mismatch repair...
  41. ncbi Carbamoyl phosphate synthetase: closure of the B-domain as a result of nucleotide binding
    J B Thoden
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin Madison 53705, USA
    Biochemistry 38:2347-57. 1999
    ..Details concerning the geometries of the two active sites contained within the large subunit of CPS are described...
  42. ncbi Structural analysis of the ZEN-4/CeMKLP1 motor domain and its interaction with microtubules
    Dilem Hizlan
    European Molecular Biology Laboratory, Structural Biology and Biocomputing Programme, Heidelberg, Germany
    J Struct Biol 153:73-84. 2006
    ..Furthermore, we investigated the location, structure, and function of a highly conserved extension of approximately 50 residues unique to the Kinesin-6 subfamily, located in the motor core loop6/beta4 region...
  43. ncbi Self-association of the Escherichia coli transcription activator MalT in the presence of maltotriose and ATP
    V Schreiber
    Unité de Génétique Moléculaire, URA CNRS 1773, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France
    J Biol Chem 274:33220-6. 1999
    ..promoters and activates transcription initiation only in the presence of maltotriose and ATP (or adenylyl imidodiphosphate (AMP-PNP))...
  44. ncbi Inhibition of an iron-responsive element/iron regulatory protein-1 complex by ATP binding and hydrolysis
    Zvezdana Popovic
    Laboratory Medicine and Pathobiology, University of Toronto, Canada
    FEBS J 274:3108-19. 2007
    ..Although the iron-responsive element does not itself hydrolyze ATP, its presence enhances iron regulatory protein-1's ATPase activity, and ATP hydrolysis results in loss of the complex in gel shift assays...
  45. ncbi Global conformational transitions in Escherichia coli primary replicative helicase DnaB protein induced by ATP, ADP, and single-stranded DNA binding. Multiple conformational states of the helicase hexamer
    M J Jezewska
    Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch at Galveston, 77555 1053, USA
    J Biol Chem 271:4261-5. 1996
    ....
  46. ncbi Asymmetry in kinesin walking
    Qiang Shao
    Department of Chemistry, Texas A and M University, College Station, Texas 77845, USA
    Biochemistry 46:9098-106. 2007
    ..We show that the possible processive walking of the heterodimeric kinesin can be explained by introducing a force-generating intermediate, the kinesin-ATP complex, which is different from the posthydrolytic species, kinesin-ADP/Pi...
  47. pmc Coupling between ATP binding and DNA cleavage by DNA topoisomerase II: A unifying kinetic and structural mechanism
    Felix Mueller-Planitz
    Department of Biochemistry, School of Medicine, Stanford University, Stanford, California 94305, USA
    J Biol Chem 283:17463-76. 2008
    ....
  48. pmc Structures of GRP94-nucleotide complexes reveal mechanistic differences between the hsp90 chaperones
    D Eric Dollins
    Hauptman Woodward Medical Research Institute, 700 Ellicott Street, Buffalo, NY 14203, USA
    Mol Cell 28:41-56. 2007
    ..Our observations provide a structural explanation for GRP94's observed rate of ATP hydrolysis and suggest a model for the role of ATP binding and hydrolysis in the GRP94 chaperone cycle...
  49. ncbi A Grp on the Hsp90 mechanism
    Klaus Richter
    Center for Integrative Protein Science, Department Chemie, Technische Universitat Munchen, Lichtenbergstrasse 4, 85747 Garching, Germany
    Mol Cell 28:177-9. 2007
    ..2007) present the crystal structure of Grp94, which highlights the similarity between Grp94 and Hsp90 and provides insight into the resting state of Grp94 and potentially other Hsp90 family members...
  50. pmc Direct visualization of asymmetric adenine-nucleotide-induced conformational changes in MutL alpha
    Elizabeth J Sacho
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Cell 29:112-21. 2008
    ..These data reveal an ATPase cycle in which sequential nucleotide binding, hydrolysis, and release modulate the conformational states of MutL alpha...
  51. pmc Crystal structure of the kinase domain of serum and glucocorticoid-regulated kinase 1 in complex with AMP PNP
    Baoguang Zhao
    Department of Computational and Structural Chemistry, GlaxoSmithKline, King of Prussia, Pennsylvania 19406, USA
    Protein Sci 16:2761-9. 2007
    ..Since the differences from other kinases occur around the ATP binding site, this structure can provide valuable insight into the design of selective and highly potent ATP-competitive inhibitors of SGK1 kinase...
  52. ncbi Two structures of the catalytic domain of phosphorylase kinase: an active protein kinase complexed with substrate analogue and product
    D J Owen
    Laboratory of Molecular Biophysics, University of Oxford, UK
    Structure 3:467-82. 1995
    ..Structural studies of phosphorylase kinase (Phk), the major substrate of which is glycogen phosphorylase, may be expected to shed light on its regulation...
  53. pmc Two distinct modes of processive kinesin movement in mixtures of ATP and AMP-PNP
    Radhika Subramanian
    Department of Biochemistry, Brandeis University, Waltham, MA 02454, USA
    J Gen Physiol 130:445-55. 2007
    ..In one of these, the enzyme can accomplish high-duty-ratio processive movement without alternating-sites ATP hydrolysis...
  54. pmc An ATP gate controls tubulin binding by the tethered head of kinesin-1
    Maria C Alonso
    Molecular Motors Group, Marie Curie Research Institute, The Chart, Oxted, Surrey RH8 0TL, UK
    Science 316:120-3. 2007
    ..The data reveal an ATP gate that operates independently of the microtubule lattice, by ATP-dependent release of a steric or allosteric block on the tubulin binding site of the tethered kinesin-ADP head...
  55. ncbi Ground state structure of F1-ATPase from bovine heart mitochondria at 1.9 A resolution
    Matthew W Bowler
    The Medical Research Council Dunn Human Nutrition Unit, Hills Road, Cambridge, UK
    J Biol Chem 282:14238-42. 2007
    ..Thus, the structure with bound azide represents the ADP inhibited state of the enzyme, and the new structure represents a ground state intermediate in the active catalytic cycle of ATP hydrolysis...
  56. pmc Destruction or potentiation of different prions catalyzed by similar Hsp104 remodeling activities
    James Shorter
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Mol Cell 23:425-38. 2006
    ..In vivid distinction, the endpoint of Ure2 fragmentation is short prion fibers with enhanced infectivity and self-replicating ability. These advances explain the distinct effects of Hsp104 on the inheritance of the two prions...
  57. pmc XpsE oligomerization triggered by ATP binding, not hydrolysis, leads to its association with XpsL
    Sheng Jie Shiue
    Institute of Biochemistry, National Chung Hsing University, Taichung, Taiwan, Republic of China
    EMBO J 25:1426-35. 2006
    ..For the first time, functional significance of ATP binding to GspE in type II secretion system is clearly demonstrated. The implications may also be applicable to type IV pilus biogenesis...
  58. ncbi Identifying Lys359 as a critical residue for the ATP-dependent reactions of Drosophila DNA topoisomerase II
    T Hu
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 273:9586-92. 1998
    ..Our data suggest that Lys359 is critical for the catalytic activity of topoisomerase II and may have an important function in the ATP signaling process...
  59. ncbi The role of the kinesin-13 neck in microtubule depolymerization
    Carolyn A Moores
    School of Crystallography, Birkbeck College, London, UK
    Cell Cycle 5:1812-5. 2006
    ..Our data suggest that the ring-microtubule interaction is mediated by the neck and support the idea of a role for the kinesin-13 neck in depolymerization efficiency, acting by optimizing release of tubulin from microtubule ends...
  60. ncbi Heterogeneous nucleotide occupancy stimulates functionality of phage shock protein F, an AAA+ transcriptional activator
    Nicolas Joly
    Division of Biology, Sir Alexander Fleming Building, Imperial College London, London SW7 2AZ, United Kingdom
    J Biol Chem 281:34997-5007. 2006
    ..Esigma54 complex provides clear biochemical evidence for heterogeneous nucleotide occupancy in this AAA+ protein. Based on our data, we propose a stochastic nucleotide binding and a coordinated hydrolysis mechanism in PspF1-275 hexamers...
  61. ncbi Allosteric effects of nucleotide cofactors on Escherichia coli Rep helicase-DNA binding
    I Wong
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110
    Science 256:350-5. 1992
    ....
  62. ncbi Structural Analysis of E. coli hsp90 reveals dramatic nucleotide-dependent conformational rearrangements
    Andrew K Shiau
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of California, San Francisco, 94158, USA
    Cell 127:329-40. 2006
    ....
  63. ncbi Structure of dimeric SecA, the Escherichia coli preprotein translocase motor
    Yannis Papanikolau
    Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, PO Box 1385, 71110 Heraklion, Greece
    J Mol Biol 366:1545-57. 2007
    ..The repercussion of these findings on translocase assembly and catalysis is discussed...
  64. doi Packaging of a unit-length viral genome: the role of nucleotides and the gpD decoration protein in stable nucleocapsid assembly in bacteriophage lambda
    Qin Yang
    Department of Pharmaceutical Sciences, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, C238, Denver, CO 80262, USA
    J Mol Biol 383:1037-48. 2008
    ....
  65. ncbi Binding of Escherichia coli primary replicative helicase DnaB protein to single-stranded DNA. Long-range allosteric conformational changes within the protein hexamer
    M J Jezewska
    Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch at Galveston 77555 1053, USA
    Biochemistry 35:2129-45. 1996
    ..The significance of these results for a mechanistic model of the functioning of the DnaB helicase in DNA replication is discussed...
  66. pmc Interaction of tolbutamide and cytosolic nucleotides in controlling the ATP-sensitive K+ channel in mouse beta-cells
    C Schwanstecher
    Institute of Pharmacology and Toxicology, , Germany
    Br J Pharmacol 111:302-10. 1994
    ..The latter effect increases the proportion of KATP- channels staying in the nucleotide-blocked state. In addition, this state potentiates the direct effect of sulphonylureas...
  67. pmc Adrenocorticotropic hormone and cAMP inhibit noninactivating K+ current in adrenocortical cells by an A-kinase-independent mechanism requiring ATP hydrolysis
    J J Enyeart
    Department of Pharmacology, Ohio State University, College of Medicine, Columbus 43210 1239, USA
    J Gen Physiol 108:251-64. 1996
    ..The simultaneous activation of A-kinase- and Ca(2+)-dependent pathways produces the full steroidogenic response...
  68. pmc Effects of divalent cations on the potency of ATP and related agonists in the rat isolated vagus nerve: implications for P2 purinoceptor classification
    D J Trezise
    Glaxo Institute of Applied Pharmacology, Department of Pharmacology, University of Cambridge
    Br J Pharmacol 113:463-70. 1994
    ..The possible implications of these findings to the present system for subclassifying P2 purinoceptors are profound.Keywords: P2 purinoceptors; rat vagus nerve; depolarization; ATP; 2-methylthioATP; alpha, beta-methylene ATP..
  69. ncbi Kinetic mechanism of adenine nucleotide binding to and hydrolysis by the Escherichia coli Rep monomer. 2. Application of a kinetic competition approach
    K J Moore
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110
    Biochemistry 33:14565-78. 1994
    ..abstract truncated at 400 words)..
  70. pmc P2-, but not P1-purinoceptors mediate formation of 1, 4, 5-inositol trisphosphate and its metabolites via a pertussis toxin-insensitive pathway in the rat renal cortex
    C Nanoff
    Institute of Pharmacology, University of Vienna, Austria
    Br J Pharmacol 100:63-8. 1990
    ..On the other hand, our results suggest that renal A,-adenosine receptors do not use stimulation of phosphoinositide breakdown as a transmembrane signalling system...
  71. ncbi Evidence for two separate vasoconstriction-mediating nucleotide receptors, both distinct from the P2x-receptor, in rabbit basilar artery: a receptor for pyrimidine nucleotides and a receptor for purine nucleotides
    I von Kugelgen
    Pharmakologisches Institut, Freiburg, Federal Republic of Germany
    Naunyn Schmiedebergs Arch Pharmacol 341:538-46. 1990
    ..Reactive blue 2 reduced neither the UTP- nor the ATP-induced vasoconstriction.(ABSTRACT TRUNCATED AT 250 WORDS)..
  72. doi Identification of an unusual AT(D)Pase-like activity in multifunctional NAD glycohydrolase from the venom of Agkistrodon acutus
    Liyun Zhang
    Department of Chemistry, University of Science and Technology of China, Hefei, Anhui, PR China
    Biochimie 91:240-51. 2009
    ..AMP is an efficient inhibitor against NAD. AA-NADase has so far been identified as the first unique multicatalytic enzyme with both NADase and AT(D)Pase-like activities...
  73. pmc A cyclic GMP-dependent calcium-activated chloride current in smooth-muscle cells from rat mesenteric resistance arteries
    Vladimir V Matchkov
    The Water and Salt Research Center and Department of Physiology, University of Aarhus, Aarhus, Denmark
    J Gen Physiol 123:121-34. 2004
    ....
  74. ncbi Modulation of IH by 5-HT in neonatal rat motoneurones in vitro: mediation through a phosphorylation independent action of cAMP
    P M Larkman
    Department of Pharmacology, University of Edinburgh, U K
    Neuropharmacology 36:721-33. 1997
    ..The 5-HT receptor subtype mediating this response cannot be correlated with any of the classified 5-HT receptor subtypes that stimulate adenylyl cyclase...
  75. ncbi Ligand-induced conformational transitions in Escherichia coli phosphofructokinase 2: evidence for an allosteric site for MgATP2-
    V Guixe
    Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Santiago
    Biochemistry 37:13269-75. 1998
    ..These results demonstrate the presence of an allosteric site for MgATP2- in Pfk-2 from E. coli, responsible for the inhibition of the enzyme activity by this ligand...
  76. pmc Characterization of purinoceptors mediating depolarization of rat isolated vagus nerve
    D J Trezise
    Glaxo Institute of Applied Pharmacology, Department of Pharmacology, University of Cambridge
    Br J Pharmacol 110:1055-60. 1993
    ..Although it appears that other purinoceptors are present,these data suggest that the rat vagus nerve may serve as a useful preparation for studying the pharmacology of neuronal P2x receptors...
  77. pmc Stimulation by extracellular ATP and UTP of the mitogen-activated protein kinase cascade and proliferation of rat renal mesangial cells
    A Huwiler
    Department of Pharmacology, University of Basel, Switzerland
    Br J Pharmacol 113:1455-63. 1994
    ..7. In summary, these results suggest that ATP and UTP trigger the activation of the mitogen-activated protein kinase signalling cascade in mesangial cells and this may be responsible for the potent mitogenic activity of both nucleotides...
  78. ncbi A mutation in GroEL interferes with protein folding by reducing the rate of discharge of sequestered polypeptides
    F Baneyx
    Molecular Biology Division, E I Du Pont de Nemours and Company, Wilmington, Delaware 19880 0402
    J Biol Chem 267:11637-44. 1992
    ..Pulse-chase experiments involving a double immunoabsorption technique confirmed that Rbu-P2-carboxylase remained associated two times longer with GroEL140 than with GroEL in vivo...
  79. pmc Crystal structures of Escherichia coli topoisomerase IV ParE subunit (24 and 43 kilodaltons): a single residue dictates differences in novobiocin potency against topoisomerase IV and DNA gyrase
    Steven Bellon
    Vertex Pharmaceuticals Incorporated, Cambridge, Massachusetts 02139, USA
    Antimicrob Agents Chemother 48:1856-64. 2004
    ..These data demonstrate that the ParE M74 and GyrB I78 side chains impart opposite effects on the enzyme's substrate affinity and catalytic efficiency...
  80. ncbi Kinetic mechanism of the single-stranded DNA recognition by Escherichia coli replicative helicase DnaB protein. Application of the matrix projection operator technique to analyze stopped-flow kinetics
    W Bujalowski
    Department of Human Biological Chemistry, The University of Texas Medical Branch at Galveston, Galveston, TX, 77555 1053, USA
    J Mol Biol 295:831-52. 2000
    ..The significance of these results for the functioning of the DnaB helicase is discussed...
  81. ncbi Crystal structure of argininosuccinate synthetase from Thermus thermophilus HB8. Structural basis for the catalytic action
    Masaru Goto
    Department of Chemistry, Graduate School of Science, Osaka City University, Osaka 558 8585, Japan
    J Biol Chem 277:15890-6. 2002
    ..thermophilus HB8 in its free form, complexed with intact ATP, and complexed with an ATP analogue (adenylyl imidodiphosphate) and substrate analogues (arginine and succinate) have been determined at 2.3-, 2.3-, and 1...
  82. pmc Evidence for active interactions between microfilaments and microtubules in myxomycete flagellates
    T Q Uyeda
    Department of Biology, Faculty of Science, University of Tokyo, Japan
    J Cell Biol 108:1727-35. 1989
    ..Thus, the pharmacological characteristics of this motility were partly similar to, although not the same as, those of the known microtubule-dependent motilities...
  83. ncbi Strand specificity in the interactions of Escherichia coli primary replicative helicase DnaB protein with a replication fork
    M J Jezewska
    Department of Human Biological Chemistry and Genetics, The University of Texas Medical Branch at Galveston, 301 University Boulevard, Galveston, Texas 77555 1053, USA
    Biochemistry 36:10320-6. 1997
    ..The significance of the these results for a mechanistic model of the hexameric DnaB helicase action is discussed...
  84. ncbi Selective activation of cystic fibrosis transmembrane conductance regulator Cl- and HCO3- conductances
    M M Reddy
    Department of Pediatrics, UCSD School of Medicine, La Jolla, CA 92093 0831, USA
    JOP 2:212-8. 2001
    ..The non-hydrolysable AMP-PNP (5'-adenylyl imidodiphosphate) cannot substitute for ATP in activating HCO(3)(-) conductance...
  85. ncbi Closed structure of phosphoglycerate kinase from Thermotoga maritima reveals the catalytic mechanism and determinants of thermal stability
    G Auerbach
    Max Planck Institut fur Biochemie, Abt Strukturforschung, 82152, Martinsried, Germany
    Structure 5:1475-83. 1997
    ..It adds to a series of four known crystal structures of PGKs from mesophilic via moderately thermophilic to a hyperthermophilic organism, allowing a detailed analysis of possible structural determinants of thermostability...
  86. ncbi Interactions between adaptor protein-1 of the clathrin coat and microtubules via type 1a microtubule-associated proteins
    E Orzech
    Department of Cell and Animal Biology, Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel
    J Biol Chem 276:31340-8. 2001
    ....
  87. pmc Differential scanning calorimetry study of glycerinated rabbit psoas muscle fibres in intermediate state of ATP hydrolysis
    Timea Dergez
    Institute of Bioanalysis Faculty of Medicine, H 7624 Pecs, Hungary
    BMC Struct Biol 7:41. 2007
    ..The energetics of long functioning intermediate states of ATP hydrolysis cycle was studied in muscle fibres by differential scanning calorimetry (DSC)...
  88. ncbi Molecular structure of human galactokinase: implications for type II galactosemia
    James B Thoden
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Biol Chem 280:9662-70. 2005
    ....
  89. doi Can nucleotides prevent Cu-induced oxidative damage?
    Rozena Baruch-Suchodolsky
    Department of Chemistry, Gonda Goldschmied Medical Research Center, Bar Ilan University, Ramat Gan 52900, Israel
    J Inorg Biochem 102:862-81. 2008
    ..We conclude that nucleotides at sub mM concentrations can prevent Cu-induced OH radical formation from H(2)O(2), and hence may possibly prevent oxidative damage...
  90. doi X-ray structure and microtubule interaction of the motor domain of Neurospora crassa NcKin3, a kinesin with unusual processivity
    Alexander Marx
    Max Planck Unit for Structural Molecular Biology, Notkestrasse 85, 22607 Hamburg, Germany
    Biochemistry 47:1848-61. 2008
    ..In spite of unusual extensions at the N-terminus and within flexible loops L2, L8a, and L12 (corresponding to the K-loop of monomeric kinesins), the microtubule binding geometry is similar to that of other members of the kinesin family...
  91. pmc Structural basis of APH(3')-IIIa-mediated resistance to N1-substituted aminoglycoside antibiotics
    Desiree H Fong
    Department of Biochemistry, McGill University, 3649 Promenade Sir William Osler, Montreal, Quebec, Canada
    Antimicrob Agents Chemother 53:3049-55. 2009
    ....
  92. pmc Stimulation of recombinant Ca(v)3.2, T-type, Ca(2+) channel currents by CaMKIIgamma(C)
    Joshua T Wolfe
    Department of Pharmacology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
    J Physiol 538:343-55. 2002
    ..2 channel activity (27 nM free Ca(2+)) as did replacing pipette ATP with adenylyl imidodiphosphate (AMP-PNP), a non-hydrolysable analogue...
  93. ncbi ATP-dependent regulation of SK4/IK1-like currents in rat submandibular acinar cells: possible role of cAMP-dependent protein kinase
    M Hayashi
    Laboratory of Physiology, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060 0818, Japan
    Am J Physiol Cell Physiol 286:C635-46. 2004
    ..Collectively, these results suggest that ATP-dependent regulation of native SK4/IK1-like channels, at least in part, is mediated by endogenous PKA in RSA cells...
  94. pmc The structure of an archaeal homodimeric ligase which has RNA circularization activity
    Mark Adrian Brooks
    IBBMC CNRS, Universite de Paris Sud, CNRS UMR8619, IFR115, 91405 Orsay, France
    Protein Sci 17:1336-45. 2008
    ..Additionally, we show that the protein is indeed capable of circularizing RNA molecules in an ATP-dependent reaction. These structural and biochemical results provide an insight into the potential physiological roles of Pab1020...
  95. pmc A superfamily 3 DNA helicase encoded by plasmid pSSVi from the hyperthermophilic archaeon Sulfolobus solfataricus unwinds DNA as a higher-order oligomer and interacts with host primase
    Xin Guo
    State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, People s Republic of China
    J Bacteriol 192:1853-64. 2010
    ..Yeast two-hybrid and coimmunoprecipitation assays revealed that ORF735 interacted with the noncatalytic subunit of host primase. These findings provide clues to the functional role of ORF735 in pSSVi DNA replication...
  96. pmc Zymogen activation in a reconstituted pancreatic acinar cell system
    Edwin C Thrower
    Veterans Administration Medical Center, Bldg 27, 950 Campbell Avenue, West Haven, CT 06516, USA
    Am J Physiol Gastrointest Liver Physiol 290:G894-902. 2006
    ..A component of the activation of some zymogens, such as chymotrypsinogen and procarboxypeptidase, may depend on ATP but not on trypsin or cathepsin B...
  97. pmc Structural evidence for substrate-induced synergism and half-sites reactivity in biotin carboxylase
    Igor Mochalkin
    Pfizer Global Research and Development, Ann Arbor, Michigan 48105, USA
    Protein Sci 17:1706-18. 2008
    ..The liganded subunit was in the closed or catalytic conformation while the unliganded subunit was in the open conformation. This provides the first structural evidence for half-sites reactivity in BC...
  98. ncbi Structural characterization of adenine nucleotides bound to Escherichia coli adenylate kinase. 1. Adenosine conformations by proton two-dimensional transferred nuclear Overhauser effect spectroscopy
    Y Lin
    Department of Physics, Indiana University Purdue University Indianapolis IUPUI, Indiana 46202 3273, USA
    Biochemistry 39:3636-46. 2000
    ..of adenosine 5'-triphosphate (ATP) and adenosine 5'-monophosphate (AMP), and of an ATP analogue, adenylyl imidodiphosphate (AMPPNP), bound to Escherichia coliadenylate kinase (AKe) in the complexes of AKe.Mg(II)ATP, AKe.AMP...
  99. ncbi Multidrug resistance protein 4 (ABCC4)-mediated ATP hydrolysis: effect of transport substrates and characterization of the post-hydrolysis transition state
    Zuben E Sauna
    Laboratory of Cell Biology, Center for Cancer Research, NCI, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland 20892 4256, USA
    J Biol Chem 279:48855-64. 2004
    ....
  100. ncbi Bovine F1-ATPase covalently inhibited with 4-chloro-7-nitrobenzofurazan: the structure provides further support for a rotary catalytic mechanism
    G L Orriss
    Medical Research Council, Laboratory of Molecular Biology, Cambridge, UK
    Structure 6:831-7. 1998
    ..To understand more about the catalytic mechanisms, F1-ATPase was inhibited by reaction with 4-chloro-7-nitrobenzofurazan (NBD-Cl) and the structure of the inhibited complex (F1-NBD) determined by X-ray crystallography...
  101. pmc Mutating the converter-relay interface of Drosophila myosin perturbs ATPase activity, actin motility, myofibril stability and flight ability
    William A Kronert
    Department of Biology, Molecular Biology Institute and Heart Institute, San Diego State University, San Diego, CA 92182 4614, USA
    J Mol Biol 398:625-32. 2010
    ..Our results provide the first mutational evidence that intramolecular signaling between the relay loop and converter domain is critical for myosin function both in vitro and in muscle...

Research Grants69

  1. High Resolution Labels for EM
    James F Hainfeld; Fiscal Year: 2010
    ..The ultimate aim of these studies is to understand life processes, which in turn, can lead to effective treatment of disease. ..
  2. KINETIC STUDIES OF ENZYME MECHANISMS
    WILLIAM CLELAND; Fiscal Year: 2004
    ..9) Other collaborations will determine isotope effects on cytidine deaminase, non-enzymatic decarboxylation of hydroxybenzoates, and on selected fiavoprotein-catalyzed reactions. ..
  3. MYOFIBRILLAR STRUCTURE OF STRIATED MUSCLE
    MICHAEL KAY REEDY; Fiscal Year: 2010
    ..A Thick filament and A-band structure in relaxed IFM will be collaboratively modeled from our X-ray data at 2, 20 and 40 (normal flight temperature in vivo), using our supporting EM studies to generate model geometry. ..
  4. MYOFIBRILLAR STRUCTURE OF STRIATED MUSCLE
    Michael Reedy; Fiscal Year: 2007
    ..abstract_text> ..
  5. REGULATION OF THE INSULIN RECEPTOR KINASE
    Jeffrey Pessin; Fiscal Year: 1991
    ....
  6. DYNAMIC EVENTS IN MYOSIN DURING CONTRACTION OF MUSCLE
    Emil Reisler; Fiscal Year: 1990
    ..The research proposed in this application will bring an improved understanding of contractile processes in muscle and non-muscle cells...
  7. MYOFIBRILLAR STRUCTURE OF STRIATED MUSCLE
    Michael Reedy; Fiscal Year: 2005
    ..Thick filament and A-band structure in relaxed IFM will be collaboratively modeled from our X-ray data at 2o, 20o and 40o (normal flight temperature in vivo), using our supporting EM studies to generate model geometry. ..
  8. MYOFIBRILLAR STRUCTURE OF STRIATED MUSCLE
    Michael Reedy; Fiscal Year: 2006
    ..abstract_text> ..
  9. Pancreatic zymogen activation in a reconstituted system
    Edwin Thrower; Fiscal Year: 2006
    ..We plan to use this system to identify the compartment(s) responsible for zymogen processing and to examine the ATP and calcium-dependent mechanism of activation. ..
  10. Function and regulation of cardiac Na transporters
    Donald Hilgemann; Fiscal Year: 2007
    ..4) In parallel with the experimental program, we will develop improved mathematical models of cardiac ion homeostasis with an emphasis on these three Na transporters. ..
  11. ENZYMATIC MECHANISMS OF SULFUR NUCLEOSIDE METABOLISM
    GEORGE MARKHAM; Fiscal Year: 2007
    ..These studies will unmask why nature conserves use of a pyruvoyl cofactor for this metabolic function instead of adopting the common pyridoxal cofactor. ..
  12. IMP Dehydrogenase and the Hydra of Cancer Chemotherapy
    GEORGE MARKHAM; Fiscal Year: 2007
    ..These results will reveal the inner working of an essential enzyme and whether there is promise for enhancing clinical efficacy through combinations of known drugs or the discovery of novel subdomain ligands. ..
  13. ENZYMATIC MECHANISMS OF SULFUR-NUCLEOSIDE METABOLISM
    GEORGE MARKHAM; Fiscal Year: 1993
    ..The roles of the divalent metal ions which are required for activity will be determined from paramagnetic resonance experiments which will reveal the ligands to the metal ions and the distance between them...