Genomes and Genes
Summary: 5'-Adenylic acid, monoanhydride with imidodiphosphoric acid. An analog of ATP, in which the oxygen atom bridging the beta to the gamma phosphate is replaced by a nitrogen atom. It is a potent competitive inhibitor of soluble and membrane-bound mitochondrial ATPase and also inhibits ATP-dependent reactions of oxidative phosphorylation.
Publications223 found, 100 shown here
- Flexibility in the ABC transporter MsbA: Alternating access with a twistAndrew Ward
Departments of Cell Biology and Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, CB 105, La Jolla, CA 92037, USA
Proc Natl Acad Sci U S A 104:19005-10. 2007..Altogether, the conformational changes between these structures suggest that large ranges of motion may be required for substrate transport...
- Drosophila kinesin motor domain extending to amino acid position 392 is dimeric when expressed in Escherichia coliT G Huang
Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213
J Biol Chem 269:16502-7. 1994..Electron microscopy indicates that both DKH340 monomers and DKH392 dimers decorate microtubules with a periodicity of 8 nm...
- Structure of the multidrug ABC transporter Sav1866 from Staphylococcus aureus in complex with AMP-PNPRoger J P Dawson
Institute of Molecular Biology and Biophysics, ETH Zurich HPK D14 3, 8093 Zurich, Switzerland
FEBS Lett 581:935-8. 2007..The results confirm that the ATP-bound state of multidrug ABC transporters is coupled to an outward-facing conformation of the transmembrane domains...
- Mutation of Walker-A lysine 464 in cystic fibrosis transmembrane conductance regulator reveals functional interaction between its nucleotide-binding domainsAllan C Powe
Department of Physiology and Dalton Cardiovascular Research Center, University of Missouri Columbia, 134 Research Park Drive, Columbia, MO 65211, USA
J Physiol 539:333-46. 2002..Delay of closing in wild type by adenylyl imidodiphosphate (AMP-PNP), a non-hydrolysable ATP analogue, is markedly diminished in K464A mutants due to reduction in ..
- Differential and simultaneous adenosine di- and triphosphate binding by MutSKeith P Bjornson
Department of Biochemistry and Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710, USA
J Biol Chem 278:18557-62. 2003..These findings suggest that the protein exists primarily as the ATP.MutS.ADP ternary complex in solution and that this may be the form of the protein that is involved in DNA encounters in vivo...
- Nucleotide-dependent single- to double-headed binding of kinesinK Kawaguchi
Department of Physics, School of Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan
Science 291:667-9. 2001..Thus, kinesin binds through two heads in the former and one head in the latter two states, which supports a major prediction of the hand-over-hand model...
- The alternating ATPase domains of MutS control DNA mismatch repairMeindert H Lamers
Division of Molecular Carcinogenesis, Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
EMBO J 22:746-56. 2003..We propose that asymmetry of the ATPase domains is an essential feature of mismatch repair that controls the timing of the different steps in the repair cascade...
- Differential interactions of nucleotides at the two nucleotide binding domains of the cystic fibrosis transmembrane conductance regulatorL Aleksandrov
S. C. Johnson Medical Research Center, Mayo Clinic Scottsdale, Scottsdale, Arizona 85259, USA
J Biol Chem 276:12918-23. 2001..This argues strongly that AMP-PNP does not inhibit ATP hydrolysis at NBD2 and thereby questions the relevance of hydrolysis at that domain to channel closing...
- Crystal structure of an N-terminal fragment of the DNA gyrase B proteinD B Wigley
Department of Chemistry, York University, Heslington, UK
Nature 351:624-9. 1991..The C-terminal domains form the sides of a 20 A hole through the protein dimer which may play a role in DNA strand passage during the supercoiling reaction...
- Release of isolated single kinesin molecules from microtubulesY Vugmeyster
Department of Biochemistry and The Center for Complex Systems, Brandeis University, Waltham, Massachusetts 02254, USA
Biochemistry 37:747-57. 1998..The inhibitor adenylyl imidodiphosphate (AMP-PNP) induces stochastic pauses in the movement of bead-labeled enzyme molecules in 1 mM ATP...
- A human topoisomerase II alpha heterodimer with only one ATP binding site can go through successive catalytic cyclesCamilla Skouboe
Department of Molecular Biology, University of Aarhus, C F Møllers Alle, Building 130, Arhus C 8000, Denmark
J Biol Chem 278:5768-74. 2003..The results can be explained in a model, where efficient catalysis requires an extensive communication between the second ATP and the DNA segment to be cleaved...
- Nucleotide-dependent domain movement in the ATPase domain of a human type IIA DNA topoisomeraseHua Wei
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
J Biol Chem 280:37041-7. 2005..Comparison of these structures revealed rigid-body movement of the structural modules within the ATPase domain, suggestive of the motions of a molecular gate...
- A Grp on the Hsp90 mechanismKlaus Richter
Center for Integrative Protein Science, Department Chemie, Technische Universitat Munchen, Lichtenbergstrasse 4, 85747 Garching, Germany
Mol Cell 28:177-9. 2007..2007) present the crystal structure of Grp94, which highlights the similarity between Grp94 and Hsp90 and provides insight into the resting state of Grp94 and potentially other Hsp90 family members...
- Crystal structure of the kinase domain of serum and glucocorticoid-regulated kinase 1 in complex with AMP PNPBaoguang Zhao
Department of Computational and Structural Chemistry, GlaxoSmithKline, King of Prussia, Pennsylvania 19406, USA
Protein Sci 16:2761-9. 2007..Since the differences from other kinases occur around the ATP binding site, this structure can provide valuable insight into the design of selective and highly potent ATP-competitive inhibitors of SGK1 kinase...
- Dissociation of the single-ring chaperonin GroEL by high hydrostatic pressureMarkandeswar Panda
Department of Biochemistry, Mail Code 7760, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, Texas 78229 3900, USA
Biochemistry 41:12843-9. 2002..The results presented in this investigation demonstrate that the presence of a second ring in the GroEL 14mer is important for its stability in an environment where the functional ligands of the chaperonin are available...
- Temperature and ligand dependence of conformation and helical order in myosin filamentsS Xu
Laboratory of Muscle Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
Biochemistry 42:390-401. 2003..Low temperatures favor one disordered conformation, while high temperatures favor the ordered (closed) conformation together with a second disordered conformation...
- Identifying Lys359 as a critical residue for the ATP-dependent reactions of Drosophila DNA topoisomerase IIT Hu
Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
J Biol Chem 273:9586-92. 1998..Our data suggest that Lys359 is critical for the catalytic activity of topoisomerase II and may have an important function in the ATP signaling process...
- The nucleotide-binding domains of P-glycoprotein. Functional symmetry in the isolated domain demonstrated by N-ethylmaleimide labellingGeorgina Berridge
Nuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford, UK
Eur J Biochem 270:1483-92. 2003..Any observed functional asymmetry in the intact transporter presumably reflects different rates of hydrolysis at the two NBDs or interdomain communications...
- ATP analogue binding to the A subunit induces conformational changes in the E subunit that involves a disulfide bond formation in plant V-ATPaseY Kawamura
Cryobiosystem Research Center, Iwate University, Iwate, Japan
Eur J Biochem 268:2801-9. 2001..This is the first demonstration of interaction between the A and E subunits in the substrate-binding state of a plant V-ATPase...
- Carbamoyl phosphate synthetase: closure of the B-domain as a result of nucleotide bindingJ B Thoden
Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin Madison 53705, USA
Biochemistry 38:2347-57. 1999..Details concerning the geometries of the two active sites contained within the large subunit of CPS are described...
- Transformation of MutL by ATP binding and hydrolysis: a switch in DNA mismatch repairC Ban
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
Cell 97:85-97. 1999..Dimerization of the LN40 region is required for and is the rate-limiting step in ATP hydrolysis by MutL. The ATPase activity of MutL is stimulated by DNA and likely acts as a switch to coordinate DNA mismatch repair...
- Structural analyses of nucleotide binding to an aminoglycoside phosphotransferaseD L Burk
Antimicrobial Research Centre and Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada L8N 3Z5
Biochemistry 40:8756-64. 2001..This alternate binding mode is likely a conserved feature among aminoglycoside kinases and could be exploited for the structure-based drug design of compounds to combat antibiotic resistance...
- Structure of the AAA ATPase p97X Zhang
Molecular Structure and Function Laboratory, Imperial Cancer Research Fund, London SW7 2AY, United Kingdom
Mol Cell 6:1473-84. 2000..A comparison with NSF D2 (ATP complex) reveals possible conformational changes induced by ATP hydrolysis. Given the D1 and D2 packing arrangement, we propose a ratchet mechanism for p97 during its ATP hydrolysis cycle...
- pH-controlled quaternary states of hexameric DnaB helicaseL E Donate
Campus de Cantoblanco, Centro Nacional de Biotecnologia CSIC, Madrid, 28049, Spain
J Mol Biol 303:383-93. 2000..Structural consequences of this quaternary polymorphism, as well as its functional implications for helicase activity, are discussed...
- Coupling between ATP binding and DNA cleavage by DNA topoisomerase II: A unifying kinetic and structural mechanismFelix Mueller-Planitz
Department of Biochemistry, School of Medicine, Stanford University, Stanford, California 94305, USA
J Biol Chem 283:17463-76. 2008....
- ATP binding to the first nucleotide-binding domain of multidrug resistance protein MRP1 increases binding and hydrolysis of ATP and trapping of ADP at the second domainYue Xian Hou
Mayo Foundation, S C Johnson Medical Research Center, Mayo Clinic Scottsdale, Scottsdale, Arizona 85259, USA
J Biol Chem 277:5110-9. 2002..The interactions between the two asymmetric NBDs of MRP1 protein may enhance the catalytic efficiency of the MRP1 protein and hence of its ATP-dependent transport of conjugated anions out of cells...
- Direct visualization of asymmetric adenine-nucleotide-induced conformational changes in MutL alphaElizabeth J Sacho
Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
Mol Cell 29:112-21. 2008..These data reveal an ATPase cycle in which sequential nucleotide binding, hydrolysis, and release modulate the conformational states of MutL alpha...
- Distinguishing inchworm and hand-over-hand processive kinesin movement by neck rotation measurementsWei Hua
Biophysics and Structural Biology Program, Biochemistry Department, Brandeis University, Waltham, MA 02454 9110, USA
Science 295:844-8. 2002..In fact, there were no rotations, a finding that is inconsistent with symmetric hand-over-hand movement. An alternative "inchworm" mechanism is consistent with our experimental results...
- The role of ATP hydrolysis for kinesin processivityChristopher M Farrell
Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
J Biol Chem 277:17079-87. 2002..This mechanism optimizes forward movement and processivity by ensuring that one motor domain is tightly bound to the microtubule before the second can detach...
- PurT-encoded glycinamide ribonucleotide transformylase. Accommodation of adenosine nucleotide analogs within the active siteJames B Thoden
Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
J Biol Chem 277:23898-908. 2002..This sensitivity to nucleotide identity is in sharp contrast to that observed for the "P-loop"-containing enzymes, in which the conformation of the binding motif is virtually unchanged in the presence or absence of nucleotides...
- Self-association of the Escherichia coli transcription activator MalT in the presence of maltotriose and ATPV Schreiber
Unité de Génétique Moléculaire, URA CNRS 1773, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France
J Biol Chem 274:33220-6. 1999..promoters and activates transcription initiation only in the presence of maltotriose and ATP (or adenylyl imidodiphosphate (AMP-PNP))...
- A mechanism for microtubule depolymerization by KinI kinesinsCarolyn A Moores
Department of Cell Biology, CB227, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA
Mol Cell 9:903-9. 2002..Thus, the relatively small class-specific differences within the KinI motor core modulate a fundamentally conserved mode of interaction with microtubules to produce a unique depolymerizing activity...
- Two distinct modes of processive kinesin movement in mixtures of ATP and AMP-PNPRadhika Subramanian
Department of Biochemistry, Brandeis University, Waltham, MA 02454, USA
J Gen Physiol 130:445-55. 2007..In one of these, the enzyme can accomplish high-duty-ratio processive movement without alternating-sites ATP hydrolysis...
- Regulated cycling of mitochondrial Hsp70 at the protein import channelQinglian Liu
Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
Science 300:139-41. 2003..Consistent with the ratchet model of translocation, subsequent hydrolysis of ATP would trap the polypeptide, driving import by preventing its movement back toward the cytosol...
- Structures of GRP94-nucleotide complexes reveal mechanistic differences between the hsp90 chaperonesD Eric Dollins
Hauptman Woodward Medical Research Institute, 700 Ellicott Street, Buffalo, NY 14203, USA
Mol Cell 28:41-56. 2007..Our observations provide a structural explanation for GRP94's observed rate of ATP hydrolysis and suggest a model for the role of ATP binding and hydrolysis in the GRP94 chaperone cycle...
- Structural Analysis of E. coli hsp90 reveals dramatic nucleotide-dependent conformational rearrangementsAndrew K Shiau
Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of California, San Francisco, 94158, USA
Cell 127:329-40. 2006....
- Nucleotide exchange from the high-affinity ATP-binding site in SecA is the rate-limiting step in the ATPase cycle of the soluble enzyme and occurs through a specialized conformational stateJohn J Fak
Department of Biological Sciences, 702A Fairchild Center, MC2434, Columbia University, New York, New York 10027, USA
Biochemistry 43:7307-27. 2004....
- Two structures of the catalytic domain of phosphorylase kinase: an active protein kinase complexed with substrate analogue and productD J Owen
Laboratory of Molecular Biophysics, University of Oxford, UK
Structure 3:467-82. 1995..Structural studies of phosphorylase kinase (Phk), the major substrate of which is glycogen phosphorylase, may be expected to shed light on its regulation...
- The role of the kinesin-13 neck in microtubule depolymerizationCarolyn A Moores
School of Crystallography, Birkbeck College, London, UK
Cell Cycle 5:1812-5. 2006..Our data suggest that the ring-microtubule interaction is mediated by the neck and support the idea of a role for the kinesin-13 neck in depolymerization efficiency, acting by optimizing release of tubulin from microtubule ends...
- Structure of the photolyase-like domain of cryptochrome 1 from Arabidopsis thalianaChad A Brautigam
Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9050, USA
Proc Natl Acad Sci U S A 101:12142-7. 2004..ATP, and we observe a single molecule of an ATP analog bound in the aforementioned surface cavity, near the bound FAD cofactor. The structure has implications for the signaling mechanism of CRY blue-light photoreceptors...
- Inter-domain motions of the N-domain of the KdpFABC complex, a P-type ATPase, are not driven by ATP-induced conformational changesMelina Haupt
Institut fur Organische Chemie und Biochemie, Technische Universitat Munchen, Lichtenbergstr 4, 85747 Garching, Germany
J Mol Biol 342:1547-58. 2004..No evidence of significant conformational changes was observed within the N-domain upon nucleotide binding, thus ruling out a role for ATP-induced conformational changes in the reaction cycle...
- Destruction or potentiation of different prions catalyzed by similar Hsp104 remodeling activitiesJames Shorter
Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
Mol Cell 23:425-38. 2006..In vivid distinction, the endpoint of Ure2 fragmentation is short prion fibers with enhanced infectivity and self-replicating ability. These advances explain the distinct effects of Hsp104 on the inheritance of the two prions...
- Function-dependent conformational changes of the ABCG2 multidrug transporter modify its interaction with a monoclonal antibody on the cell surfaceCsilla Ozvegy-Laczka
National Medical Center, Institute of Haematology and Immunology, Membrane Research Group and Hungarian Academy of Sciences, 1113 Budapest, Hungary
J Biol Chem 280:4219-27. 2005....
- XpsE oligomerization triggered by ATP binding, not hydrolysis, leads to its association with XpsLSheng Jie Shiue
Institute of Biochemistry, National Chung Hsing University, Taichung, Taiwan, Republic of China
EMBO J 25:1426-35. 2006..For the first time, functional significance of ATP binding to GspE in type II secretion system is clearly demonstrated. The implications may also be applicable to type IV pilus biogenesis...
- Structural analysis of the ZEN-4/CeMKLP1 motor domain and its interaction with microtubulesDilem Hizlan
European Molecular Biology Laboratory, Structural Biology and Biocomputing Programme, Heidelberg, Germany
J Struct Biol 153:73-84. 2006..Furthermore, we investigated the location, structure, and function of a highly conserved extension of approximately 50 residues unique to the Kinesin-6 subfamily, located in the motor core loop6/beta4 region...
- Asymmetry in kinesin walkingQiang Shao
Department of Chemistry, Texas A and M University, College Station, Texas 77845, USA
Biochemistry 46:9098-106. 2007..We show that the possible processive walking of the heterodimeric kinesin can be explained by introducing a force-generating intermediate, the kinesin-ATP complex, which is different from the posthydrolytic species, kinesin-ADP/Pi...
- Allosteric effects of nucleotide cofactors on Escherichia coli Rep helicase-DNA bindingI Wong
Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110
Science 256:350-5. 1992....
- Structure of dimeric SecA, the Escherichia coli preprotein translocase motorYannis Papanikolau
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, PO Box 1385, 71110 Heraklion, Greece
J Mol Biol 366:1545-57. 2007..The repercussion of these findings on translocase assembly and catalysis is discussed...
- A kinesin-like motor inhibits microtubule dynamic instabilityHenrik Bringmann
Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, Meyerhofstrabetae 1, 69117 Heidelberg, Germany
Science 303:1519-22. 2004..The integration of the two properties, motility and inhibition of microtubule dynamics, in one molecule emphasizes the versatile properties of kinesin family members...
- Thermodynamic characterization of the binding of nucleotides to glycyl-tRNA synthetaseJohn David Dignam
Department of Biochemistry and Molecular Biology, Medical College of Ohio, 3035 Arlington Avenue, Toledo, Ohio 43614 5804, USA
Biochemistry 42:5333-40. 2003..The results are consistent with a significant conformational transition in glycyl-tRNA synthetase coupled to the binding of GSAd...
- Discrimination of ATP, ADP, and AMPPNP by chaperonin GroEL: hexokinase treatment revealed the exclusive role of ATPFumihiro Motojima
Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Midori ku, Yokohama 226 8503, Japan
J Biol Chem 278:26648-54. 2003..In the absence of hexokinase, apparent cis folding of rhodanese and malate dehydrogenase was observed in ADP and AMPPNP. Thus, the exclusive role of ATP in generation of the cis ternary complex is now evident...
- Interactions of the Escherichia coli DnaB helicase hexamer with the replication factor the DnaC protein. Effect of nucleotide cofactors and the ssDNA on protein-protein interactions and the topology of the complexRoberto Galletto
Department of Human Biological Chemistry and Genetics, The University of Texas Medical Branch at Galveston, Galveston, TX 77555 1053, USA
J Mol Biol 329:441-65. 2003..In solution, the DnaC protein-binding site is located on the large 33 kDa domain of the DnaB helicase. The significance of the results in the functioning of the DnaB helicase-DnaC protein complex is discussed...
- Inhibition of an iron-responsive element/iron regulatory protein-1 complex by ATP binding and hydrolysisZvezdana Popovic
Laboratory Medicine and Pathobiology, University of Toronto, Canada
FEBS J 274:3108-19. 2007..Although the iron-responsive element does not itself hydrolyze ATP, its presence enhances iron regulatory protein-1's ATPase activity, and ATP hydrolysis results in loss of the complex in gel shift assays...
- Crystal structures of active fully assembled substrate- and product-bound complexes of UDP-N-acetylmuramic acid:L-alanine ligase (MurC) from Haemophilus influenzaeClifford D Mol
Syrrx Inc, San Diego, California 92121, USA
J Bacteriol 185:4152-62. 2003....
- An ATP gate controls tubulin binding by the tethered head of kinesin-1Maria C Alonso
Molecular Motors Group, Marie Curie Research Institute, The Chart, Oxted, Surrey RH8 0TL, UK
Science 316:120-3. 2007..The data reveal an ATP gate that operates independently of the microtubule lattice, by ATP-dependent release of a steric or allosteric block on the tubulin binding site of the tethered kinesin-ADP head...
- Global conformational transitions in Escherichia coli primary replicative helicase DnaB protein induced by ATP, ADP, and single-stranded DNA binding. Multiple conformational states of the helicase hexamerM J Jezewska
Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch at Galveston, 77555 1053, USA
J Biol Chem 271:4261-5. 1996....
- Structural insight into nucleotide recognition in tau-protein kinase I/glycogen synthase kinase 3 betaMasaaki Aoki
Mitsubishi Kagaku Institute of Life Sciences MITILS, 11 Minamiohya, Machida, Tokyo 194 8511, Japan
Acta Crystallogr D Biol Crystallogr 60:439-46. 2004....
- Ground state structure of F1-ATPase from bovine heart mitochondria at 1.9 A resolutionMatthew W Bowler
The Medical Research Council Dunn Human Nutrition Unit, Hills Road, Cambridge, UK
J Biol Chem 282:14238-42. 2007..Thus, the structure with bound azide represents the ADP inhibited state of the enzyme, and the new structure represents a ground state intermediate in the active catalytic cycle of ATP hydrolysis...
- Modulation of kinesin binding by the C-termini of tubulinGeorgios Skiniotis
European Molecular Biology Laboratory, Meyerhofstrasse 1, Heidelberg, Germany
EMBO J 23:989-99. 2004..Overall, our study reveals that the tubulin C-termini define the stability of the MT-kinesin complex in a nucleotide-dependent manner, and highlights the involvement of tubulin in the regulation of weak and strong kinesin binding states...
- Heterogeneous nucleotide occupancy stimulates functionality of phage shock protein F, an AAA+ transcriptional activatorNicolas Joly
Division of Biology, Sir Alexander Fleming Building, Imperial College London, London SW7 2AZ, United Kingdom
J Biol Chem 281:34997-5007. 2006..Esigma54 complex provides clear biochemical evidence for heterogeneous nucleotide occupancy in this AAA+ protein. Based on our data, we propose a stochastic nucleotide binding and a coordinated hydrolysis mechanism in PspF1-275 hexamers...
- Packaging of a unit-length viral genome: the role of nucleotides and the gpD decoration protein in stable nucleocapsid assembly in bacteriophage lambdaQin Yang
Department of Pharmaceutical Sciences, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, C238, Denver, CO 80262, USA
J Mol Biol 383:1037-48. 2008....
- Binding of Escherichia coli primary replicative helicase DnaB protein to single-stranded DNA. Long-range allosteric conformational changes within the protein hexamerM J Jezewska
Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch at Galveston 77555 1053, USA
Biochemistry 35:2129-45. 1996..The significance of these results for a mechanistic model of the functioning of the DnaB helicase in DNA replication is discussed...
- Interaction of tolbutamide and cytosolic nucleotides in controlling the ATP-sensitive K+ channel in mouse beta-cellsC Schwanstecher
Institute of Pharmacology and Toxicology, , Germany
Br J Pharmacol 111:302-10. 1994..The latter effect increases the proportion of KATP- channels staying in the nucleotide-blocked state. In addition, this state potentiates the direct effect of sulphonylureas...
- Adrenocorticotropic hormone and cAMP inhibit noninactivating K+ current in adrenocortical cells by an A-kinase-independent mechanism requiring ATP hydrolysisJ J Enyeart
Department of Pharmacology, Ohio State University, College of Medicine, Columbus 43210 1239, USA
J Gen Physiol 108:251-64. 1996..The simultaneous activation of A-kinase- and Ca(2+)-dependent pathways produces the full steroidogenic response...
- Effects of divalent cations on the potency of ATP and related agonists in the rat isolated vagus nerve: implications for P2 purinoceptor classificationD J Trezise
Glaxo Institute of Applied Pharmacology, Department of Pharmacology, University of Cambridge
Br J Pharmacol 113:463-70. 1994..The possible implications of these findings to the present system for subclassifying P2 purinoceptors are profound.Keywords: P2 purinoceptors; rat vagus nerve; depolarization; ATP; 2-methylthioATP; alpha, beta-methylene ATP..
- Kinetic mechanism of adenine nucleotide binding to and hydrolysis by the Escherichia coli Rep monomer. 2. Application of a kinetic competition approachK J Moore
Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, Missouri 63110
Biochemistry 33:14565-78. 1994..abstract truncated at 400 words)..
- P2-, but not P1-purinoceptors mediate formation of 1, 4, 5-inositol trisphosphate and its metabolites via a pertussis toxin-insensitive pathway in the rat renal cortexC Nanoff
Institute of Pharmacology, University of Vienna, Austria
Br J Pharmacol 100:63-8. 1990..On the other hand, our results suggest that renal A,-adenosine receptors do not use stimulation of phosphoinositide breakdown as a transmembrane signalling system...
- Evidence for two separate vasoconstriction-mediating nucleotide receptors, both distinct from the P2x-receptor, in rabbit basilar artery: a receptor for pyrimidine nucleotides and a receptor for purine nucleotidesI von Kugelgen
Pharmakologisches Institut, Freiburg, Federal Republic of Germany
Naunyn Schmiedebergs Arch Pharmacol 341:538-46. 1990..Reactive blue 2 reduced neither the UTP- nor the ATP-induced vasoconstriction.(ABSTRACT TRUNCATED AT 250 WORDS)..
- A cyclic GMP-dependent calcium-activated chloride current in smooth-muscle cells from rat mesenteric resistance arteriesVladimir V Matchkov
The Water and Salt Research Center and Department of Physiology, University of Aarhus, Aarhus, Denmark
J Gen Physiol 123:121-34. 2004....
- Identification of an unusual AT(D)Pase-like activity in multifunctional NAD glycohydrolase from the venom of Agkistrodon acutusLiyun Zhang
Department of Chemistry, University of Science and Technology of China, Hefei, Anhui, PR China
Biochimie 91:240-51. 2009..AMP is an efficient inhibitor against NAD. AA-NADase has so far been identified as the first unique multicatalytic enzyme with both NADase and AT(D)Pase-like activities...
- Modulation of IH by 5-HT in neonatal rat motoneurones in vitro: mediation through a phosphorylation independent action of cAMPP M Larkman
Department of Pharmacology, University of Edinburgh, U K
Neuropharmacology 36:721-33. 1997..The 5-HT receptor subtype mediating this response cannot be correlated with any of the classified 5-HT receptor subtypes that stimulate adenylyl cyclase...
- Ligand-induced conformational transitions in Escherichia coli phosphofructokinase 2: evidence for an allosteric site for MgATP2-V Guixe
Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Santiago
Biochemistry 37:13269-75. 1998..These results demonstrate the presence of an allosteric site for MgATP2- in Pfk-2 from E. coli, responsible for the inhibition of the enzyme activity by this ligand...
- Characterization of purinoceptors mediating depolarization of rat isolated vagus nerveD J Trezise
Glaxo Institute of Applied Pharmacology, Department of Pharmacology, University of Cambridge
Br J Pharmacol 110:1055-60. 1993..Although it appears that other purinoceptors are present,these data suggest that the rat vagus nerve may serve as a useful preparation for studying the pharmacology of neuronal P2x receptors...
- A mutation in GroEL interferes with protein folding by reducing the rate of discharge of sequestered polypeptidesF Baneyx
Molecular Biology Division, E I Du Pont de Nemours and Company, Wilmington, Delaware 19880 0402
J Biol Chem 267:11637-44. 1992..Pulse-chase experiments involving a double immunoabsorption technique confirmed that Rbu-P2-carboxylase remained associated two times longer with GroEL140 than with GroEL in vivo...
- Stimulation by extracellular ATP and UTP of the mitogen-activated protein kinase cascade and proliferation of rat renal mesangial cellsA Huwiler
Department of Pharmacology, University of Basel, Switzerland
Br J Pharmacol 113:1455-63. 1994..7. In summary, these results suggest that ATP and UTP trigger the activation of the mitogen-activated protein kinase signalling cascade in mesangial cells and this may be responsible for the potent mitogenic activity of both nucleotides...
- Kinetic mechanism of the single-stranded DNA recognition by Escherichia coli replicative helicase DnaB protein. Application of the matrix projection operator technique to analyze stopped-flow kineticsW Bujalowski
Department of Human Biological Chemistry, The University of Texas Medical Branch at Galveston, Galveston, TX, 77555 1053, USA
J Mol Biol 295:831-52. 2000..The significance of these results for the functioning of the DnaB helicase is discussed...
- Crystal structures of Escherichia coli topoisomerase IV ParE subunit (24 and 43 kilodaltons): a single residue dictates differences in novobiocin potency against topoisomerase IV and DNA gyraseSteven Bellon
Vertex Pharmaceuticals Incorporated, Cambridge, Massachusetts 02139, USA
Antimicrob Agents Chemother 48:1856-64. 2004..These data demonstrate that the ParE M74 and GyrB I78 side chains impart opposite effects on the enzyme's substrate affinity and catalytic efficiency...
- Crystal structure of argininosuccinate synthetase from Thermus thermophilus HB8. Structural basis for the catalytic actionMasaru Goto
Department of Chemistry, Graduate School of Science, Osaka City University, Osaka 558 8585, Japan
J Biol Chem 277:15890-6. 2002..thermophilus HB8 in its free form, complexed with intact ATP, and complexed with an ATP analogue (adenylyl imidodiphosphate) and substrate analogues (arginine and succinate) have been determined at 2.3-, 2.3-, and 1...
- Evidence for active interactions between microfilaments and microtubules in myxomycete flagellatesT Q Uyeda
Department of Biology, Faculty of Science, University of Tokyo, Japan
J Cell Biol 108:1727-35. 1989..Thus, the pharmacological characteristics of this motility were partly similar to, although not the same as, those of the known microtubule-dependent motilities...
- Selective activation of cystic fibrosis transmembrane conductance regulator Cl- and HCO3- conductancesM M Reddy
Department of Pediatrics, UCSD School of Medicine, La Jolla, CA 92093 0831, USA
JOP 2:212-8. 2001..The non-hydrolysable AMP-PNP (5'-adenylyl imidodiphosphate) cannot substitute for ATP in activating HCO(3)(-) conductance...
- Strand specificity in the interactions of Escherichia coli primary replicative helicase DnaB protein with a replication forkM J Jezewska
Department of Human Biological Chemistry and Genetics, The University of Texas Medical Branch at Galveston, 301 University Boulevard, Galveston, Texas 77555 1053, USA
Biochemistry 36:10320-6. 1997..The significance of the these results for a mechanistic model of the hexameric DnaB helicase action is discussed...
- Closed structure of phosphoglycerate kinase from Thermotoga maritima reveals the catalytic mechanism and determinants of thermal stabilityG Auerbach
Max Planck Institut fur Biochemie, Abt Strukturforschung, 82152, Martinsried, Germany
Structure 5:1475-83. 1997..It adds to a series of four known crystal structures of PGKs from mesophilic via moderately thermophilic to a hyperthermophilic organism, allowing a detailed analysis of possible structural determinants of thermostability...
- Interactions between adaptor protein-1 of the clathrin coat and microtubules via type 1a microtubule-associated proteinsE Orzech
Department of Cell and Animal Biology, Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel
J Biol Chem 276:31340-8. 2001....
- Differential scanning calorimetry study of glycerinated rabbit psoas muscle fibres in intermediate state of ATP hydrolysisTimea Dergez
Institute of Bioanalysis Faculty of Medicine, H 7624 Pecs, Hungary
BMC Struct Biol 7:41. 2007..The energetics of long functioning intermediate states of ATP hydrolysis cycle was studied in muscle fibres by differential scanning calorimetry (DSC)...
- Molecular structure of human galactokinase: implications for type II galactosemiaJames B Thoden
Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
J Biol Chem 280:9662-70. 2005....
- X-ray structure and microtubule interaction of the motor domain of Neurospora crassa NcKin3, a kinesin with unusual processivityAlexander Marx
Max Planck Unit for Structural Molecular Biology, Notkestrasse 85, 22607 Hamburg, Germany
Biochemistry 47:1848-61. 2008..In spite of unusual extensions at the N-terminus and within flexible loops L2, L8a, and L12 (corresponding to the K-loop of monomeric kinesins), the microtubule binding geometry is similar to that of other members of the kinesin family...
- Can nucleotides prevent Cu-induced oxidative damage?Rozena Baruch-Suchodolsky
Department of Chemistry, Gonda Goldschmied Medical Research Center, Bar Ilan University, Ramat Gan 52900, Israel
J Inorg Biochem 102:862-81. 2008..We conclude that nucleotides at sub mM concentrations can prevent Cu-induced OH radical formation from H(2)O(2), and hence may possibly prevent oxidative damage...
- Structural basis of APH(3')-IIIa-mediated resistance to N1-substituted aminoglycoside antibioticsDesiree H Fong
Department of Biochemistry, McGill University, 3649 Promenade Sir William Osler, Montreal, Quebec, Canada
Antimicrob Agents Chemother 53:3049-55. 2009....
- Stimulation of recombinant Ca(v)3.2, T-type, Ca(2+) channel currents by CaMKIIgamma(C)Joshua T Wolfe
Department of Pharmacology, University of Virginia School of Medicine, Charlottesville, VA 22908, USA
J Physiol 538:343-55. 2002..2 channel activity (27 nM free Ca(2+)) as did replacing pipette ATP with adenylyl imidodiphosphate (AMP-PNP), a non-hydrolysable analogue...
- ATP-dependent regulation of SK4/IK1-like currents in rat submandibular acinar cells: possible role of cAMP-dependent protein kinaseM Hayashi
Laboratory of Physiology, Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060 0818, Japan
Am J Physiol Cell Physiol 286:C635-46. 2004..Collectively, these results suggest that ATP-dependent regulation of native SK4/IK1-like channels, at least in part, is mediated by endogenous PKA in RSA cells...
- The structure of an archaeal homodimeric ligase which has RNA circularization activityMark Adrian Brooks
IBBMC CNRS, Universite de Paris Sud, CNRS UMR8619, IFR115, 91405 Orsay, France
Protein Sci 17:1336-45. 2008..Additionally, we show that the protein is indeed capable of circularizing RNA molecules in an ATP-dependent reaction. These structural and biochemical results provide an insight into the potential physiological roles of Pab1020...
- A superfamily 3 DNA helicase encoded by plasmid pSSVi from the hyperthermophilic archaeon Sulfolobus solfataricus unwinds DNA as a higher-order oligomer and interacts with host primaseXin Guo
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, People s Republic of China
J Bacteriol 192:1853-64. 2010..Yeast two-hybrid and coimmunoprecipitation assays revealed that ORF735 interacted with the noncatalytic subunit of host primase. These findings provide clues to the functional role of ORF735 in pSSVi DNA replication...
- Zymogen activation in a reconstituted pancreatic acinar cell systemEdwin C Thrower
Veterans Administration Medical Center, Bldg 27, 950 Campbell Avenue, West Haven, CT 06516, USA
Am J Physiol Gastrointest Liver Physiol 290:G894-902. 2006..A component of the activation of some zymogens, such as chymotrypsinogen and procarboxypeptidase, may depend on ATP but not on trypsin or cathepsin B...
- Multidrug resistance protein 4 (ABCC4)-mediated ATP hydrolysis: effect of transport substrates and characterization of the post-hydrolysis transition stateZuben E Sauna
Laboratory of Cell Biology, Center for Cancer Research, NCI, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland 20892 4256, USA
J Biol Chem 279:48855-64. 2004....
- Structural characterization of adenine nucleotides bound to Escherichia coli adenylate kinase. 1. Adenosine conformations by proton two-dimensional transferred nuclear Overhauser effect spectroscopyY Lin
Department of Physics, Indiana University Purdue University Indianapolis IUPUI, Indiana 46202 3273, USA
Biochemistry 39:3636-46. 2000..of adenosine 5'-triphosphate (ATP) and adenosine 5'-monophosphate (AMP), and of an ATP analogue, adenylyl imidodiphosphate (AMPPNP), bound to Escherichia coliadenylate kinase (AKe) in the complexes of AKe.Mg(II)ATP, AKe.AMP...
- Structural evidence for substrate-induced synergism and half-sites reactivity in biotin carboxylaseIgor Mochalkin
Pfizer Global Research and Development, Ann Arbor, Michigan 48105, USA
Protein Sci 17:1706-18. 2008..The liganded subunit was in the closed or catalytic conformation while the unliganded subunit was in the open conformation. This provides the first structural evidence for half-sites reactivity in BC...
- Interaction of iron regulatory protein-1 (IRP-1) with ATP/ADP maintains a non-IRE-binding stateZvezdana Popovic
Laboratory Medicine and Pathobiology, University of Toronto, 1 King s College Circle, Toronto, Ontario, Canada
Biochem J 430:315-24. 2010..Under normal cellular conditions, it is predicted that ATP/ADP will maintain IRP-1 in a non-IRE-binding state...
- Mutating the converter-relay interface of Drosophila myosin perturbs ATPase activity, actin motility, myofibril stability and flight abilityWilliam A Kronert
Department of Biology, Molecular Biology Institute and Heart Institute, San Diego State University, San Diego, CA 92182 4614, USA
J Mol Biol 398:625-32. 2010..Our results provide the first mutational evidence that intramolecular signaling between the relay loop and converter domain is critical for myosin function both in vitro and in muscle...
- Active-site residues of Escherichia coli DNA gyrase required in coupling ATP hydrolysis to DNA supercoiling and amino acid substitutions leading to novobiocin resistanceChristian H Gross
Vertex Pharmaceuticals Incorporated, Cambridge, Massachusetts 02139, USA
Antimicrob Agents Chemother 47:1037-46. 2003..Four new residues (D73, G77, I78, and T165) that, when changed to the appropriate amino acid, result in both significant levels of novobiocin resistance and maintain in vivo function were identified in E. coli...
- Bovine F1-ATPase covalently inhibited with 4-chloro-7-nitrobenzofurazan: the structure provides further support for a rotary catalytic mechanismG L Orriss
Medical Research Council, Laboratory of Molecular Biology, Cambridge, UK
Structure 6:831-7. 1998..To understand more about the catalytic mechanisms, F1-ATPase was inhibited by reaction with 4-chloro-7-nitrobenzofurazan (NBD-Cl) and the structure of the inhibited complex (F1-NBD) determined by X-ray crystallography...
- Formation of the productive ATP-Mg2+-bound dimer of GlcV, an ABC-ATPase from Sulfolobus solfataricusGrégory Verdon
Laboratory of Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
J Mol Biol 334:255-67. 2003..Our data suggest that ABC-ATPases with partially degenerated catalytic machineries, as they occur in vivo, can still form productive dimers to drive transport...
- High Resolution Labels for EMJames F Hainfeld; Fiscal Year: 2012..The ultimate aim of these studies is to understand life processes, which in turn, can lead to effective treatment of disease. ..
- MYOFIBRILLAR STRUCTURE OF STRIATED MUSCLEMICHAEL KAY REEDY; Fiscal Year: 2010..A Thick filament and A-band structure in relaxed IFM will be collaboratively modeled from our X-ray data at 2, 20 and 40 (normal flight temperature in vivo), using our supporting EM studies to generate model geometry. ..
- GATING OF THE CFTR CL CHANNEL BY ATP HYDROLYSISTzyh Chang Hwang; Fiscal Year: 1999....
- CHARACTERIZATION OF RNA POL II TRANSCRIPTION PROTEINSGyanendra Kumar; Fiscal Year: 1991..We anticipate that our studies will also help in further defining the general mechanism(s) of eucaryotic RNA transcription...
- TRANSMEMBRANE ACTIVATION OF THE INSULIN RECEPTOR KINASEJeffrey Pessin; Fiscal Year: 1992....
- Opening and Closing Mechanisms of CFTR ChannelsLASZLO CSANADY; Fiscal Year: 2009..g. AMPPNP). We will record from inside-out patches, excised from Xenopus oocytes expressing CFTR, in a fast-flow system with accurate temperature control. ..
- IMP Dehydrogenase and the Hydra of Cancer ChemotherapyGEORGE MARKHAM; Fiscal Year: 2008..These results will reveal the inner working of an essential enzyme and whether there is promise for enhancing clinical efficacy through combinations of known drugs or the discovery of novel subdomain ligands. ..
- Properties of Ion Channels that Control SecretionJOHN ENYEART; Fiscal Year: 2008..abstract_text> ..
- ENZYMATIC MECHANISMS OF SULFUR NUCLEOSIDE METABOLISMGEORGE MARKHAM; Fiscal Year: 2008..These studies will unmask why nature conserves use of a pyruvoyl cofactor for this metabolic function instead of adopting the common pyridoxal cofactor. ..
- Glucosensing neurons in health, obesity & diabetesVanessa Routh; Fiscal Year: 2008..This will facilitate the development of new and effective treatments for these serious diseases of the modern world. ..
- Probing Cell Division with Synthetic ChemistryTimothy Mitchison; Fiscal Year: 2008..Having found small molecule tools that perturb cell division by novel mechanisms, we will test their ability to kill cancer cells in vitro. ..
- COFACTOR-DEPENDENT AMINE OXIDATIONSIrene Lee; Fiscal Year: 2009....
- FUNCTION AND MECHANISM OF DNA TOPOISOMERASETao Shih Hsieh; Fiscal Year: 2010..This will also allow us to examine the mechanistic basis of a number of anti-cancer drugs that affect the equilibrium of DNA cleavage and religation. ..
- Cytoskeleton polymerization dynamics in the cell cycleTimothy J Mitchison; Fiscal Year: 2010..Understanding midbody assembles will reveal principles for generating asymmetric assemblies in the cytoplasm, and will tell us how the cleavage furrow is positioned. ..
- Structural Studies of RecA-DNA ComplexesCharles Bell; Fiscal Year: 2009..Thus, understanding the mechanism of action of RecA will contribute greatly to our general knowledge of enzymes involved in virtually all aspects of DNA metabolism. ..
- DELTAVISION MICROSCOPE SYSTEMLinda Wordeman; Fiscal Year: 2001..The operation and management of the equipment, the internal advisory committee, and institutional support for Deltavision are described. ..
- Chemical genetic analysis of intracellular TransportTarun Kapoor; Fiscal Year: 2007..It is likely that these small molecules will lead to the identification of new targets for therapeutic agents and improved treatments of human diseases. ..
- BIOPHYSICS AND ENZYMOLOGY OF KINESIN MOVEMENTJeff Gelles; Fiscal Year: 2001..The experiments will combine single-molecule biophysics techniques with conventional enzymology methods including protein chemical modification, site-directed mutagenesis, and chemical kinetics techniques. ..
- Single-molecule analysis of nucleocytoplasmic transportJeff Gelles; Fiscal Year: 2002....
- ELECTRON MICROSCOPETimothy Mitchison; Fiscal Year: 2004..The automated controls and digital image acquisition will also be optimal for introducing new users to electron microscopy and for minimizing maintenance requirements that might arise from operator inexperience. ..
- ACTIN DYNAMICS AND CELL LOCOMOTIONTimothy Mitchison; Fiscal Year: 2004..For perturbation experiments we will use protein tools, and also cell permeable chemicals coming out of a screening effort at the Harvard Institute of Chemistry and Cell Biology (ICCB). ..
- INTERACTIONS OF DNA-PK WITH THE TRANSCRIPTION APPARATUSWilliam Dynan; Fiscal Year: 2004..Experiments in this proposal will provide definitive information about signaling interactions that occur between DNA-PK and the transcription apparatus. ..
- Glucosensing Neurons in Euglycemia, Hypoglycemia & HAAFVanessa Routh; Fiscal Year: 2005..This will facilitate the development of new and effective treatments for Type I diabetes mellitus and its major modern complication hypoglycemia. ..
- Structure and regulation of the ATP synthaseDavid Mueller; Fiscal Year: 2006..abstract_text> ..
- Pancreatic zymogen activation in a reconstituted systemEdwin Thrower; Fiscal Year: 2006..We plan to use this system to identify the compartment(s) responsible for zymogen processing and to examine the ATP and calcium-dependent mechanism of activation. [unreadable] [unreadable] [unreadable]..
- Kinetic Characterization of Lon ProteaseIrene Lee; Fiscal Year: 2007..abstract_text> ..
- Laser Capture Microscopy and 2D-DIGE: Cancer ProteomicsWilliam Dynan; Fiscal Year: 2007..In the next phase, broader clinical utility will be demonstrated using a larger sample set. Changes in the pattern of protein expression will be identified. These changes can serve as clinically useful markers of tumor progression. ..