poly u

Summary

Summary: A group of uridine ribonucleotides in which the phosphate residues of each uridine ribonucleotide act as bridges in forming diester linkages between the ribose moieties.

Top Publications

  1. doi Ampligen: a potential toll-like 3 receptor adjuvant for immunotherapy of cancer
    B Jasani
    Departments of Pathology, School of Medicine, Cardiff University, Cardiff, Wales, UK
    Vaccine 27:3401-4. 2009
  2. pmc Genetic analysis of sequences in the 3' nontranslated region of hepatitis C virus that are important for RNA replication
    Peter Friebe
    Institute for Virology, Johannes Gutenberg University Mainz, 55131 Mainz, Germany
    J Virol 76:5326-38. 2002
  3. pmc Localization of spermine binding sites in 23S rRNA by photoaffinity labeling: parsing the spermine contribution to ribosomal 50S subunit functions
    Maria A Xaplanteri
    Laboratory of Biochemistry, School of Medicine, University of Patras GR 26500 Patras, Greece
    Nucleic Acids Res 33:2792-805. 2005
  4. pmc 3' nontranslated RNA signals required for replication of hepatitis C virus RNA
    Minkyung Yi
    Department of Microbiology and Immunology, The University of Texas Medical Branch at Galveston, Galveston, Texas 77555 1019, USA
    J Virol 77:3557-68. 2003
  5. pmc 3' RNA elements in hepatitis C virus replication: kissing partners and long poly(U)
    Shihyun You
    Center for the Study of Hepatitis C, Laboratory of Virology and Infectious Disease, The Rockefeller University, 1230 York Ave, New York, NY 10065, USA
    J Virol 82:184-95. 2008
  6. ncbi Structural insights into the interaction of the Nip7 PUA domain with polyuridine RNA
    Patricia P Coltri
    Center for Structural Molecular Biology, Brazilian Synchrotron Light Laboratory, LNLS, Rua Giuseppe Maximo Scolfaro 10000, P O Box 6192, CEP13083 970, Campinas SP, Brazil
    Biochemistry 46:14177-87. 2007
  7. ncbi TLR3 is essential for the induction of protective immunity against Punta Toro Virus infection by the double-stranded RNA (dsRNA), poly(I:C12U), but not Poly(I:C): differential recognition of synthetic dsRNA molecules
    Brian B Gowen
    Institute for Antiviral Research, Utah State University, Logan, Utah 84341, USA
    J Immunol 178:5200-8. 2007
  8. ncbi Intranasal immunization with H5N1 vaccine plus Poly I:Poly C12U, a Toll-like receptor agonist, protects mice against homologous and heterologous virus challenge
    Takeshi Ichinohe
    Department of Pathology, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashimurayama shi, Tokyo 208 0011, Japan
    Microbes Infect 9:1333-40. 2007
  9. ncbi Cross-protection against H5N1 influenza virus infection is afforded by intranasal inoculation with seasonal trivalent inactivated influenza vaccine
    Takeshi Ichinohe
    Department of Pathology, National Institute of Infectious Diseases, Musashimurayama shi, Tokyo, Japan
    J Infect Dis 196:1313-20. 2007
  10. ncbi Protein-primed RNA synthesis by purified poliovirus RNA polymerase
    A V Paul
    Department of Molecular Genetics and Microbiology, School of Medicine, Health Science Center, State University of New York, Stony Brook 11794 5222, USA
    Nature 393:280-4. 1998

Research Grants

  1. NUCLEOSIDE, NUCLEOTIDE, AND POLYNUCLEOTIDE ANALOGS
    Donald Bergstrom; Fiscal Year: 1980
  2. ESTROGEN ACTION IN XENOPUS LIVER
    Daniel Schoenberg; Fiscal Year: 1990
  3. Cis-acting RNA elements in hepatitis C virus replication
    Guangxiang Luo; Fiscal Year: 2003
  4. Cis-acting RNA elements in hepatitis C virus replication
    Guangxiang Luo; Fiscal Year: 2007
  5. Cis-acting RNA elements in hepatitis C virus replication
    Guangxiang Luo; Fiscal Year: 2006
  6. EXPRESSION OF CYTOCHROME P448 ENVIRONMENTAL INFLUENCES
    Edward Bresnick; Fiscal Year: 1980
  7. MECHANISMS OF CONTROL OF THE GAP-43 GENE
    Nora Perrone Bizzozero; Fiscal Year: 1999
  8. MECHANISMS OF CONTROL OF THE GAP-43 GENE
    Nora Perrone Bizzozero; Fiscal Year: 1999
  9. PROCESSING OF EUKARYOTIC MESSENGER RNA
    Mary Edmonds; Fiscal Year: 1990
  10. CONTROL OF TRANSCRIPT ELONGATION BY MAMMALIAN RNA POL II
    MICHAEL IZBAN; Fiscal Year: 1999

Detail Information

Publications189 found, 100 shown here

  1. doi Ampligen: a potential toll-like 3 receptor adjuvant for immunotherapy of cancer
    B Jasani
    Departments of Pathology, School of Medicine, Cardiff University, Cardiff, Wales, UK
    Vaccine 27:3401-4. 2009
    ....
  2. pmc Genetic analysis of sequences in the 3' nontranslated region of hepatitis C virus that are important for RNA replication
    Peter Friebe
    Institute for Virology, Johannes Gutenberg University Mainz, 55131 Mainz, Germany
    J Virol 76:5326-38. 2002
    ..These data represent the first detailed mapping of sequences in the 3' NTR assumed to act as a promoter for initiation of minus-strand RNA synthesis...
  3. pmc Localization of spermine binding sites in 23S rRNA by photoaffinity labeling: parsing the spermine contribution to ribosomal 50S subunit functions
    Maria A Xaplanteri
    Laboratory of Biochemistry, School of Medicine, University of Patras GR 26500 Patras, Greece
    Nucleic Acids Res 33:2792-805. 2005
    ..However, they exhibited higher reactivity toward puromycin and enhanced tRNA-translocation efficiency. These results suggest an essential role for polyamines in the structural and functional integrity of the large ribosomal subunit...
  4. pmc 3' nontranslated RNA signals required for replication of hepatitis C virus RNA
    Minkyung Yi
    Department of Microbiology and Immunology, The University of Texas Medical Branch at Galveston, Galveston, Texas 77555 1019, USA
    J Virol 77:3557-68. 2003
    ....
  5. pmc 3' RNA elements in hepatitis C virus replication: kissing partners and long poly(U)
    Shihyun You
    Center for the Study of Hepatitis C, Laboratory of Virology and Infectious Disease, The Rockefeller University, 1230 York Ave, New York, NY 10065, USA
    J Virol 82:184-95. 2008
    ..This suggests that the poly(U/UC) tract does not function simply as an unstructured spacer to position the kissing-loop elements...
  6. ncbi Structural insights into the interaction of the Nip7 PUA domain with polyuridine RNA
    Patricia P Coltri
    Center for Structural Molecular Biology, Brazilian Synchrotron Light Laboratory, LNLS, Rua Giuseppe Maximo Scolfaro 10000, P O Box 6192, CEP13083 970, Campinas SP, Brazil
    Biochemistry 46:14177-87. 2007
    ..This work provides experimental verification that the PUA domain mediates Nip7 interaction with RNA and reveals that the preference for interaction with polyuridine sequences is conserved in Archaea and eukaryotic Nip7 proteins...
  7. ncbi TLR3 is essential for the induction of protective immunity against Punta Toro Virus infection by the double-stranded RNA (dsRNA), poly(I:C12U), but not Poly(I:C): differential recognition of synthetic dsRNA molecules
    Brian B Gowen
    Institute for Antiviral Research, Utah State University, Logan, Utah 84341, USA
    J Immunol 178:5200-8. 2007
    ..We conclude that TLR3 is essential to the induction of antiviral activity elicited by poly(I:C(12)U), which does not appear to be recognized by the cytosolic sensor of poly(I:C), melanoma differentiation-associated gene-5...
  8. ncbi Intranasal immunization with H5N1 vaccine plus Poly I:Poly C12U, a Toll-like receptor agonist, protects mice against homologous and heterologous virus challenge
    Takeshi Ichinohe
    Department of Pathology, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashimurayama shi, Tokyo 208 0011, Japan
    Microbes Infect 9:1333-40. 2007
    ....
  9. ncbi Cross-protection against H5N1 influenza virus infection is afforded by intranasal inoculation with seasonal trivalent inactivated influenza vaccine
    Takeshi Ichinohe
    Department of Pathology, National Institute of Infectious Diseases, Musashimurayama shi, Tokyo, Japan
    J Infect Dis 196:1313-20. 2007
    ..We evaluated the ability of currently licensed seasonal influenza vaccine to confer cross-protection against highly pathogenic H5N1 influenza virus in mice...
  10. ncbi Protein-primed RNA synthesis by purified poliovirus RNA polymerase
    A V Paul
    Department of Molecular Genetics and Microbiology, School of Medicine, Health Science Center, State University of New York, Stony Brook 11794 5222, USA
    Nature 393:280-4. 1998
    ..Uridylylated VPg can then prime the transcription of polyadenylate RNA by 3Dpol to produce VPg-linked poly(U). Initiation of transcription of the poliovirus genome from the polyadenylated 3' end therefore depends on VPg...
  11. ncbi Uridine addition after microRNA-directed cleavage
    Binzhang Shen
    Department of Genetics, Harvard Medical School, and Department of Molecular Biology, Massachusetts General Hospital, 50 Blossom Street, Boston, MA 02114, USA
    Science 306:997. 2004
    ..This 3' uridine addition, as shown for Arabidopsis, is correlated with decapping and 5' shortening of the cleaved products, suggesting a mechanistic step in the miRNA-directed mRNA decay mechanism...
  12. ncbi The Cid1 poly(U) polymerase
    Olivia S Rissland
    Sir William Dunn School of Pathology, University of Oxford, UK
    Biochim Biophys Acta 1779:286-94. 2008
    ..Here, we review the literature on Cid1, other PUPs and uridylation, a conserved and previously under-appreciated mechanism of RNA regulation...
  13. ncbi The RNA structures engaged in replication and transcription of the A59 strain of mouse hepatitis virus
    D Sawicki
    Department of Microbiology and Immunology, Medical College of Ohio, 3055 Arlington Avenue, Toledo, OH 43614, USA
    J Gen Virol 82:385-96. 2001
    ..Failure originally to find TIs and TF cores was probably due to overdigestion with RNase A...
  14. ncbi RNA recognition motif-type RNA-binding proteins in Trypanosoma cruzi form a family involved in the interaction with specific transcripts in vivo
    Javier G De Gaudenzi
    Instituto de Investigaciones Biotecnológicas Instituto Tecnológico de Chascomús, CONICET UNSAM, 1650 San Martin, Provincia de Buenos Aires, Argentina
    J Biol Chem 278:18884-94. 2003
    ..Altogether, these results suggest that TcRBPs might be modulated in vivo, to favor or preclude the interaction with specific transcripts in a developmentally regulated manner...
  15. ncbi Polyadenylation regulates the stability of Trypanosoma brucei mitochondrial RNAs
    Christopher M Ryan
    Department of Microbiology and the Witebsky Center for Microbial Pathogenesis and Immunology, School of Medicine and Biomedical Sciences, State University of New York, Buffalo, New York 14214, USA
    J Biol Chem 278:32753-62. 2003
    ..These experiments demonstrate that the poly(A) tail can act as a cis-acting element to facilitate degradation of T. brucei mitochondrial mRNAs...
  16. ncbi Binding of ribosome recycling factor to ribosomes, comparison with tRNA
    Go Hirokawa
    Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 277:35847-52. 2002
    ..The binding of RRF and tRNA to ribosomes was influenced by Mg(2+) and NH(4)(+) ions in a similar manner...
  17. doi Fast in vitro translation system immobilized on a surface via specific biotinylation of the ribosome
    Romualdas Stapulionis
    Department of Physics and Astronomy, Aarhus University, Ny Munkegade, Bldg 1520, DK 8000 Arhus C, Denmark
    Biol Chem 389:1239-49. 2008
    ..Functional interactions of the immobilized ribosomes with various components of the protein synthesis apparatus are shown by use of surface plasmon resonance...
  18. ncbi Characterization of mammalian eIF2A and identification of the yeast homolog
    Wendy L Zoll
    Department of Biochemistry, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 4935, USA
    J Biol Chem 277:37079-87. 2002
    ..The lack of any apparent phenotype in the DeltaeIF2A strain suggests that eIF2A functions in a minor pathway, perhaps internal initiation or in the translation of a small number of specific mRNAs...
  19. pmc Probing the substrate specificity of Escherichia coli RNase E using a novel oligonucleotide-based assay
    Vladimir R Kaberdin
    Max F Perutz Laboratories, Department of Microbiology and Genetics, University Department at the Vienna Biocenter, Dr Bohrgasse 9 4, A 1030 Vienna, Austria
    Nucleic Acids Res 31:4710-6. 2003
    ..Moreover, the simplicity and efficiency of the proposed assay suggest that it can be a valuable tool not only for the characterization of RNase E homologs but also for the analysis of other site-specific nucleases...
  20. ncbi Mg2+ ion effect on conformational equilibrium of poly A . 2 poly U and poly A poly U in aqueous solutions
    Victor A Sorokin
    Department of Molecular Biophysics, Institute for Low Temperature Physics and Engineering, National Academy of Sciences of Ukraine, 47 Lenin Avenue, Kharkov 61103, Ukraine
    Int J Biol Macromol 31:223-33. 2003
    ..2 poly U (A2U) and poly A . poly U (AU) solutions at low (0.01 M Na(+)) and high (0.1 M Na(+)) ionic strengths...
  21. ncbi Species-specific and isoform-specific RNA binding of human and mouse fragile X mental retardation proteins
    Robert B Denman
    Laboratory of Molecular Neurobiology, Department of Molecular Biology, New York State Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, Staten Island, New York 10314, USA
    Biochem Biophys Res Commun 292:1063-9. 2002
    ..Finally, these data demonstrate that each FMRP variant binds RNAs uniquely, resulting in a set of proteins with differing affinities...
  22. pmc Functional implications of ribosomal protein L2 in protein biosynthesis as shown by in vivo replacement studies
    M Uhlein
    Max Delbruck Centrum fur Molekulare Medizin, Robert Rossle Str 10, D 13125 Berlin, Federal Republic of Germany
    Biochem J 331:423-30. 1998
    ..Replacement of this histidine residue in the human and archaebacterial proteins by glycine, arginine or alanine had no effect on ribosome assembly, but strongly reduced the translational activity of ribosomes containing these mutants...
  23. ncbi Preferential degradation of polyadenylated and polyuridinylated RNAs by the bacterial exoribonuclease polynucleotide phosphorylase
    I Lisitsky
    Department of Biology, Israel Institute of Technology, Haifa, Israel
    Eur J Biochem 261:468-74. 1999
    ..The high affinity of PNPase for polyadenylated RNA molecules may be part of the molecular mechanism by which polyadenylated RNA is preferentially degraded in bacterial cells...
  24. ncbi Kinetic and product distribution analysis of human eosinophil cationic protein indicates a subsite arrangement that favors exonuclease-type activity
    E Boix
    Departament de Bioquimica i Biologia Molecular, Facultat de Ciencies, Universitat Autonoma de Barcelona, 08193 Bellaterra, Spain
    J Biol Chem 274:15605-14. 1999
    ..The differences in the multisubsites structure could explain the reduced catalytic efficiency of ECP and the shift from an endonuclease to an exonuclease-type mechanism...
  25. ncbi Various effects of paromomycin on tmRNA-directed trans-translation
    Toshiharu Takahashi
    Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Hirosaki University, Hirosaki 036 8561 Japan
    J Biol Chem 278:27672-80. 2003
    ..The effect of paromomycin on trans-translation differs substantially from that on canonical translation, in which it induces miscoding by modulating the A site of the decoding helix of the small subunit RNA of the ribosome...
  26. ncbi Identification of an RNA binding specificity for the potential splicing factor TLS
    A Lerga
    Inserm U528, Institut Curie-Recherche, 26 rue d'Ulm, 75248 Paris, Cedex 05, France
    J Biol Chem 276:6807-16. 2001
    ..Hence, our results help define the role of the specific interaction of TLS with RNA during the splicing process of a pre-mRNA...
  27. ncbi Formation of 70S ribosomes: large activation energy is required for the adaptation of exclusively the small ribosomal subunit
    Gregor Blaha
    Max Planck Institut fur Molekulare Genetik, Berlin, Germany
    Biophys Chem 96:153-61. 2002
    ....
  28. pmc Poly(rC) binding proteins mediate poliovirus mRNA stability
    K E Murray
    Department of Microbiology, University of Colorado Health Sciences Center, Denver 80262, USA
    RNA 7:1126-41. 2001
    ....
  29. ncbi Counterion association with native and denatured nucleic acids: an experimental approach
    J Volker
    Department of Chemistry and Chemical Biology, Rutgers, The State University of New Jersey, Piscataway 08854, USA
    J Mol Biol 310:1011-25. 2001
    ..We discuss some of the broader implications of these measurements/results in terms of nucleic acid melting in multicomponent systems, in terms of probing counterion environments, and in terms of potential regulatory mechanisms...
  30. ncbi Poly(rA).poly(rU) with Ni(2+) ions at different temperatures: infrared absorption and vibrational circular dichroism spectroscopy
    V Andrushchenko
    Department of Chemistry, Faculty of Science, University of Calgary, Calgary, Alberta T2N 1N4, Canada
    J Biomol Struct Dyn 19:889-906. 2002
    ..VCD spectroscopy demonstrated significant advantages over infrared absorption or the related electronic CD spectroscopy...
  31. pmc Protein synthesis by single ribosomes
    Francesco Vanzi
    Pennsylvania Muscle Institute, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6083, USA
    RNA 9:1174-9. 2003
    ..This work opens the way for application of biophysical techniques, originally developed for the classical motor proteins, to the understanding of protein biosynthesis...
  32. ncbi Conformational change in the 16S rRNA in the Escherichia coli 70S ribosome induced by P/P- and P/E-site tRNAPhe binding
    James W Noah
    Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, North Carolina 27695 7622, USA
    Biochemistry 42:14386-96. 2003
    ....
  33. ncbi A ribonuclease from Chinese ginseng (Panax ginseng) flowers
    H X Wang
    Department of Microbiology, China Agricultural University, Beijing and Key Laboratory of Agro microbial Resource and Application, Ministry of Agriculture, Beijing, China
    Protein Expr Purif 33:195-9. 2004
    ..The characteristics of this ribonuclease were different from those of ribonuclease previously purified from ginseng roots...
  34. pmc Polyadenylation of genomic RNA and initiation of antigenomic RNA in a positive-strand RNA virus are controlled by the same cis-element
    Mark J M van Ooij
    Department of Medical Microbiology, Nijmegen Center for Molecular Life Science, Radboud University, Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands
    Nucleic Acids Res 34:2953-65. 2006
    ..The possibility of RNA replication independent of oriR and poly(A) demonstrate that highly debilitated viruses are able to survive by utilizing 'emergence', perhaps atavistic, mechanisms...
  35. ncbi Intercalation of ethidium into triple-strand poly(rA).2poly(rU): a thermodynamic and kinetic study
    B Garcia
    Departamento de Quimica, Universidad de Burgos, Plaza Misael Bañuelos s n, 09001 Burgos, Spain
    J Phys Chem B 110:16131-8. 2006
    ....
  36. ncbi [Mutation analysis of functional role of amino acid residues in domain IV of elongation factor G]
    A A Kovtun
    Mol Biol (Mosk) 40:850-6. 2006
    ..These loops are located at the tip of domain IV and close to the decoding center of the 30S ribosomal subunit upon EF-G interaction with the ribosome. The functional role of EF-G and its domain IV in ribosomal translocation is discussed...
  37. ncbi The flexible N-terminal domain of ribosomal protein L11 from Escherichia coli is necessary for the activation of stringent factor
    Rose Marie Jenvert
    School of Life Sciences, Södertörns högskola, S 141 04 Huddinge, Sweden
    J Mol Biol 365:764-72. 2007
    ..This suggests that the N-terminal domain can activate stringent factor in trans. It is also shown that this activation is dependent on unacylated tRNA...
  38. ncbi Hu proteins regulate polyadenylation by blocking sites containing U-rich sequences
    Hui Zhu
    Department of Genetics, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Biol Chem 282:2203-10. 2007
    ..These studies identify a novel group of mammalian polyadenylation regulators. Furthermore, they define a previously unknown nuclear function of Hu proteins...
  39. pmc Reengineering CCA-adding enzymes to function as (U,G)- or dCdCdA-adding enzymes or poly(C,A) and poly(U,G) polymerases
    Hyundae D Cho
    Department of Biochemistry, School of Medicine, University of Washington, Seattle, WA 98195 7350, USA
    Proc Natl Acad Sci U S A 104:54-9. 2007
    ..Collaboration between RNA and protein in the form of a ribonucleoprotein template may help to explain the evolutionary diversity of the nucleotidyltransferase family...
  40. pmc Changes in the conformation of 5S rRNA cause alterations in principal functions of the ribosomal nanomachine
    Ekaterini C Kouvela
    Laboratory of Biochemistry, School of Medicine, University of Patras, GR 26500 Patras, Greece
    Nucleic Acids Res 35:5108-19. 2007
    ..Our results support the notion that 5S rRNA serves as a signal transducer between regions of 23S rRNA responsible for principal ribosomal functions...
  41. ncbi [The nature of different influence of Cd2+ ions on the conformation of three-stranded polyU-polyA-polyU and polyI-polyA-polyI in aqueous solution]
    V A Sorokin
    Biofizika 52:1017-29. 2007
    ..study the effect of Cd2+ ions on the conformational equilibrium of three-stranded (A21, A2U) and single-stranded (poly U, poly A and poly I) polynucleotides in aqueous solutions containing 0.1 M Na+ (pH 7)...
  42. doi Role of the third strand in the binding of proflavine and pt-proflavine to poly(rA).2poly(rU): a thermodynamic and kinetic study
    Begoña García
    Departamento de Quimica, Universidad de Burgos, 09001 Burgos, Spain
    J Phys Chem B 112:7132-9. 2008
    ..Comparison with the binding of the same drugs to the duplex reveals that the occupation of the major groove in poly(rA).2poly(rU) by the third strand plays a critical role in the kinetic behavior...
  43. ncbi Ice and the origin of life
    Hauke Trinks
    Technical University Hamburg Harburg, D 21071 Hamburg, Germany
    Orig Life Evol Biosph 35:429-45. 2005
    ..Poly(A) was obtained in high yield and reached nucleotide chain lengths up to 400 containing predominantly 3'--> 5' linkages...
  44. ncbi Chiral selection in oligoadenylate formation in the presence of a metal ion catalyst or poly(U) template
    Kazuomi Osawa
    Department of Applied Chemistry, Gunma University, Kiryu, Gunma, 376 8515, Japan
    Orig Life Evol Biosph 35:213-23. 2005
    ..In contrast, L-ImpA or racemic DL-ImpA polymerized far less efficiently on the poly(U) template, demonstrating that chiral selection takes place in the poly(U) template-directed oligoadenylate formation...
  45. ncbi The novel mutation K87E in ribosomal protein S12 enhances protein synthesis activity during the late growth phase in Escherichia coli
    T Hosaka
    National Food Research Institute, 2 1 12 Kannondai, Tsukuba, Ibaraki 305 8642, Japan
    Mol Genet Genomics 271:317-24. 2004
    ..We propose that the aberrant activation of protein synthesis at the late growth phase is caused by the increased stability of the ribosome...
  46. ncbi Efficient and faithful in vitro translation of natural and synthetic mRNA with human ribosomes
    Ana C Ferreras
    Centro de Investigaciones Biomédicas de la Universidad de Carabobo BIOMED UC, Facultad de Ciencias de la Salud, Maracay, Venezuela
    Int J Mol Med 13:527-36. 2004
    ..This efficient and faithful system is of interest for biochemical studies of the human translational machinery, as well as a basis for screening new drugs affecting protein synthesis in pathogenic microorganisms...
  47. ncbi Mutagenesis of glutamine 290 in Escherichia coli and mitochondrial elongation factor Tu affects interactions with mitochondrial aminoacyl-tRNAs and GTPase activity
    Senyene Eyo Hunter
    Department of Chemistry, University of North Carolina, Campus Box 3290, Chapel Hill, North Carolina 27599 3290, USA
    Biochemistry 43:6917-27. 2004
    ..Surprisingly, while E. coli EF-Tu Q290L is more active in polymerization with mitochondrial Phe-tRNA(Phe), this variant has low activity in the formation of a stable ternary complex with mitochondrial aa-tRNA...
  48. ncbi Proteomics of bovine mitochondrial RNA-binding proteins: HES1/KNP-I is a new mitochondrial resident protein
    Mikhail V Ponamarev
    Department of Metabolism and Department of Structural Biology and Biochemistry, Hospital for Sick Children Research Institute, Toronto M5G 1X8, Ontario, Canada
    J Proteome Res 4:43-52. 2005
    ..3 and may be involved in several disorders mapped to that region. Thus, HES1/KNP-I is a proven mitochondrial resident protein with apparent tight membrane association and tentative RNA-binding properties...
  49. ncbi Roles of the polypyrimidine tract and 3' noncoding region of hepatitis C virus RNA in the internal ribosome entry site-mediated translation
    H Wang
    Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Institute of Medical Microbiology, Fudan University, Shanghai, PR China
    Arch Virol 150:1085-99. 2005
    ..The data above also indicated that the modulations of the IRES-mediated translation by the HCV 3'NCR and the polyU/UC tract were in a length-independent manner...
  50. ncbi Chromatographic investigation of macromolecular affinity interactions
    Galina A Platonova
    Institute of Macromolecular Compounds, Russian Academy of Sciences, St Petersburg 199004, Russia
    J Chromatogr A 1065:75-81. 2005
    ..Polyriboadenylic acid and poly(allylamine) were immobilized on macroporous disks (CIM disks). Quantitative parameters of affinity interactions between macromolecules were established using frontal analysis at different flow rates...
  51. ncbi The stabilizing contribution of thymine in duplexes of (dA)24 with (dU)24, (dT)24, (dU12-dT12), (dU-dT)12, (dU2-dT2)6, or (dU3-dT3)4: nearest neighbor and next-nearest neighbor effects
    Frank B Howard
    Laboratory of Molecular Biology, NIDDK, NIH, Bethesda, MD 20892, USA
    Biopolymers 78:221-9. 2005
    ..14 to 0.16, about the same or slightly less than values reported for polynucleotide duplexes and less than predicted from theoretical calculations...
  52. ncbi Binding studies of cationic uridyl ribonucleic guanidine (RNG) to DNA
    Myunji Park
    Department of Chemistry and Biochemistry, University of California at Santa Barbara, CA 93106, USA
    Bioorg Med Chem Lett 15:3247-51. 2005
    ..Results of the hybridization properties, sequence specificity, and the global conformation studies of the RNG U5.DNA A5 duplex are described...
  53. pmc Mechanical studies of single ribosome/mRNA complexes
    Francesco Vanzi
    Pennsylvania Muscle Institute, Department of Bioengineering, and Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Biophys J 89:1909-19. 2005
    ..The persistence length of poly(U) RNA homopolymer, modeled as a worm-like chain, was found to be 0.79 +/- 0.05 nm and the backbone elasticity was 900 +/- 140 pN, similar to values for single-stranded DNA...
  54. ncbi New features of the ribosome and ribosomal inhibitors: non-enzymatic recycling, misreading and back-translocation
    Witold Szaflarski
    Max Planck Institut fur Molekulare Genetik, AG Ribosomen, Ihnestr 73, D 14195 Berlin, Germany
    J Mol Biol 380:193-205. 2008
    ....
  55. ncbi Poly(A)- and poly(U)-specific RNA 3' tail shortening by E. coli ribonuclease E
    H Huang
    Department of Genetics, Stanford University School of Medicine, California 94305 5120, USA
    Nature 391:99-102. 1998
    ..The ability of RNase E to shorten poly(A) tails, together with the effect of tail length on endonucleolytic cleavage within primary transcripts, suggests a mechanism by which RNase E may exercise overall control over RNA decay...
  56. pmc A family of cold-regulated RNA-binding protein genes in the cyanobacterium Anabaena variabilis M3
    N Sato
    Laboratory of Life Science, Tokyo Gakugei University, Japan
    Nucleic Acids Res 23:2161-7. 1995
    ..Gel-filtration assay showed that all of the Rbp proteins have higher affinities to poly(G) and poly(U) than to poly(A) and poly(C)...
  57. pmc Characterization of RNA binding specificity of the Drosophila sex-lethal protein by in vitro ligand selection
    E Sakashita
    Department of Biology, Faculty of Science, Kobe University, Japan
    Nucleic Acids Res 22:4082-6. 1994
    ..These results suggest that Sxl recognizes and preferentially binds to a polyuridine stretch with a downstream AG sequence...
  58. ncbi Intrinsic molecular activities of the interferon-induced 2-5A-dependent RNase
    B Dong
    Department of Cancer Biology, Research Institute, Cleveland Clinic Foundation, Ohio 44195
    J Biol Chem 269:14153-8. 1994
    ..Reduced rates of 2-5A-dependent RNA cleavage were observed even after removal of ATP and chelation of divalent cations. However, optimal RNA cleavage rates required the presence of either manganese or magnesium and ATP...
  59. ncbi Affinity labelling of rat liver ribosomal protein S26 by heptauridylate containing a 5'-terminal alkylating group
    J Stahl
    Mol Biol Rep 9:219-22. 1984
    ..The affinity labelling reaction could be abolished by (pU)14 and poly(U). From the results it is concluded that ribosomal protein S26 is located at the mRNA binding site of rat liver ribosomes...
  60. pmc Conformational transitions of duplex and triplex nucleic acid helices: thermodynamic analysis of effects of salt concentration on stability using preferential interaction coefficients
    J P Bond
    Department of Chemistry, University of Wisconsin Madison 53706
    Biophys J 67:825-36. 1994
    ..With the best fitted values of ass and bss, our calculations of delta gamma PB are in close quantitative agreement with experimental observations on each of five nucleic acid order-disorder transitions...
  61. ncbi Sex lethal controls dosage compensation in Drosophila by a non-splicing mechanism
    R L Kelley
    Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Nature 387:195-9. 1997
    ..Thus, this well characterized alternative splicing factor regulates at least one target transcript by a distinct mechanism...
  62. ncbi The host factor required for RNA phage Qbeta RNA replication in vitro. Intracellular location, quantitation, and purification by polyadenylate-cellulose chromatography
    G G Carmichael
    J Biol Chem 250:3607-612. 1975
    ....
  63. pmc 3' Terminal oligo U-tract-mediated stimulation of decapping
    Man Gen Song
    Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, New Jersey 08854 8082, USA
    RNA 13:2356-65. 2007
    ..These data suggest that following post-transcriptional oligo uridylation of an mRNA or mRNA fragment, the U-tract has the capacity to specifically stimulate 5' end decapping to expedite mRNA decay...
  64. ncbi Chemical shift perturbation studies of the interactions of the second RNA-binding domain of the Drosophila sex-lethal protein with the transformer pre-mRNA polyuridine tract and 3' splice-site sequences
    S W Chi
    Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejon, Korea
    Eur J Biochem 260:649-60. 1999
    ..Taken together, the present results indicate that the interface residues that bind with GU8 and CUAGUG are much the same, but the role of the Y214 residue is clearly different between these two target sequences...
  65. ncbi Effects of Escherichia coli ribosomal protein S12 mutations on cell-free protein synthesis
    Namthip Chumpolkulwong
    RIKEN Genomic Sciences Center, Tsurumi, Yokohama, Japan
    Eur J Biochem 271:1127-34. 2004
    ..Furthermore, when 14 mouse cDNA sequences were used as test templates, the protein yields of nine templates in the K42T system were 1.2-2 times higher than that of the wild-type...
  66. ncbi Effect of different mutations in ribosomal protein S5 of Escherichia coli on translational fidelity
    W Piepersberg
    Mol Gen Genet 140:91-100. 1975
    ..The part of the S5 polypeptide involved in control of translational fidelity is different from regions altered in spectinomycin resistant strains and in the alanyl-tRNA synthetase suppressor mutant...
  67. ncbi Effects of mutagenesis of Gln97 in the switch II region of Escherichia coli elongation factor Tu on its interaction with guanine nucleotides, elongation factor Ts, and aminoacyl-tRNA
    Tomas Navratil
    Department of Chemistry, Campus Box 3290, University of North Carolina, Chapel Hill, North Carolina 27599 3290, USA
    Biochemistry 42:13587-95. 2003
    ..Strikingly, the Q97P variant was completely inactive in ternary complex formation, accounting for its inability to function in polymerization. The structural basis of these observations is discussed...
  68. ncbi Peptide elongation factor 1 from yeasts: purification and biochemical characterization of peptide elongation factors 1 alpha and 1 beta (gamma) from Saccharomyces carlsbergensis and Schizosaccharomyces pombe
    M Miyazaki
    Department of Molecular Biology, School of Science, Nagoya University, Aichi
    J Biochem 103:508-21. 1988
    ..pombe. Furthermore, a considerable amount of Phe-tRNA binding activity was distributed in the EF-1H (probably EF-1 alpha beta gamma) fraction from freshly prepared cell-free extracts of yeast...
  69. ncbi Phosphorylation of valyl-tRNA synthetase and elongation factor 1 in response to phorbol esters is associated with stimulation of both activities
    R C Venema
    Department of Biochemistry, University of California, Riverside 92521
    J Biol Chem 266:11993-8. 1991
    ..These data suggest that stimulation of translational rates in response to PMA is mediated, at least in part, by phosphorylation of valyl-tRNA synthetase and EF-1...
  70. ncbi Phosphorylation of elongation factor 1 (EF-1) and valyl-tRNA synthetase by protein kinase C and stimulation of EF-1 activity
    R C Venema
    Department of Biochemistry, University of California, Riverside 92521
    J Biol Chem 266:12574-80. 1991
    ..Thus, phosphorylation and activation of EF-1 by protein kinase C, which has been shown to occur in vitro as well as in reticulocytes, may have a role in PMA stimulation of translational rates...
  71. ncbi LRP130, a single-stranded DNA/RNA-binding protein, localizes at the outer nuclear and endoplasmic reticulum membrane, and interacts with mRNA in vivo
    Naoto Tsuchiya
    Biochemistry Division, National Cancer Center Research Institute, 5 1 1 Tsukiji Chuo ku, Tokyo 104 0045, Japan
    Biochem Biophys Res Commun 317:736-43. 2004
    ..Taking all these results together, it is suggested that LRP130, a novel type of RNA-binding protein, associates with mRNA/mRNP complexes at the outside of NE and ER, and plays a role in control of mRNA metabolisms...
  72. ncbi Lack of correlation between interferon levels induced by polyribonucleotides and their antimetastatic effect
    M Sakurai
    Department of Internal Medicine, National Cancer Center Hospital, Tokyo, Japan
    Oncology 47:251-6. 1990
    ..More extensive study of poly(A)poly(U) might give more fruitful results, which will give valuable information for future clinical trials of this lowly toxic promising agent...
  73. ncbi Heterogeneous nuclear ribonucleoprotein K contributes to angiotensin II stimulation of vascular endothelial growth factor mRNA translation
    Denis Feliers
    Dept of Medicine Nephrology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr, San Antonio, TX, USA
    Am J Physiol Renal Physiol 293:F607-15. 2007
    ..This study shows that hnRNP K augments efficiency of VEGF mRNA translation stimulated by ANG II...
  74. ncbi Nicotinic stimulation modulates tyrosine hydroxylase mRNA half-life and protein binding to the 3'UTR in a manner that requires transcription
    David F Roe
    Department of Neurobiology and Anatomy, The University of Texas Medical School, 5631 Fannin Street, Houston, TX 77030, USA
    Brain Res Mol Brain Res 120:91-102. 2004
    ..Thus, nicotinic stimulation produces a transcription-dependent increase in TH mRNA half-life that is mediated by previously unrecognized TH mRNA binding proteins...
  75. ncbi The KRAB zinc finger gene ZNF74 encodes an RNA-binding protein tightly associated with the nuclear matrix
    B Grondin
    Institut de Recherches Cliniques de Montreal, Universite de Montreal, Montreal, Quebec H2W 1R7, Canada
    J Biol Chem 271:15458-67. 1996
    ..The RNA binding properties of this protein and its tight association with the nuclear matrix, a subnuclear compartment involved in DNA replication as well as RNA synthesis and processing, suggest a role for ZNF74 in RNA metabolism...
  76. ncbi The multimeric nonstructural NS2 proteins of bluetongue virus, African horsesickness virus, and epizootic hemorrhagic disease virus differ in their single-stranded RNA-binding ability
    J M Uitenweerde
    Department of Genetics, University of Pretoria, South Africa
    Virology 209:624-32. 1995
    ..The ability of the NS2 proteins to form virus inclusion body-like structures in baculovirus-infected cells is not affected by the ssRNA-binding disparity...
  77. ncbi Interaction of antimalarial drug quinacrine with nucleic acids of variable sequence studied by spectroscopic methods
    L Rivas
    Instituto de Estructura de la Materia, CSIC, Serrano, Madrid, Spain
    J Biomol Struct Dyn 18:371-83. 2000
    ..The interaction of QA with the above nucleic acids lead to a different change in the chain stability and flexibility which is further related to the different denaturation tendency of the polymer in the presence of the metal surface...
  78. ncbi An RNA helicase, DDX1, interacting with poly(A) RNA and heterogeneous nuclear ribonucleoprotein K
    Hui Chin Chen
    Institute of Molecular Medicine, College of Medicine, National Taiwan University, Academia Sinica, No 1 Sec 4, Roosevelt Road, Taipei 106, Taiwan
    J Biol Chem 277:40403-9. 2002
    ..Moreover, the immunoprecipitated DDX1 complex but not purified DDX1 can unwind double-stranded RNA having single-stranded poly(A) overhangs...
  79. pmc Protein-primed and de novo initiation of RNA synthesis by norovirus 3Dpol
    Jacques Rohayem
    Institut fur Virologie, The Calicilab, Fiedlerstrasse 42, D 01307 Dresden, Germany
    J Virol 80:7060-9. 2006
    ..We propose a novel model for initiation of replication of the norovirus genome by 3D(pol), with a VPg-protein-primed initiation of replication of polyadenylated genomic RNA and a de novo initiation of replication of antigenomic RNA...
  80. ncbi Polyadenylation in Arabidopsis and Chlamydomonas organelles: the input of nucleotidyltransferases, poly(A) polymerases and polynucleotide phosphorylase
    Sarah L Zimmer
    Boyce Thompson Institute for Plant Research, Tower Rd, Ithaca, NY 14853, USA
    Plant J 59:88-99. 2009
    ..Together, our results identify several Ntr-PAPs and PNPase in organelle polyadenylation, and reveal novel poly(U)-rich sequences in Chlamydomonas mitochondria...
  81. ncbi Structural and functional studies of the rat mitochondrial single strand DNA binding protein P16
    G D Hoke
    Department of Biochemistry and Molecular Biophysics, Virginia Commonwealth University, Richmond 23298
    Arch Biochem Biophys 282:116-24. 1990
    ..Furthermore, the amino-terminal sequence was found to be strikingly similar to that of the Xenopus laevis mtSSB-1 and to a lesser extent similar to E. coli SSB and E. coli F sex factor SSB...
  82. ncbi Recombinant dengue virus type 1 NS3 protein exhibits specific viral RNA binding and NTPase activity regulated by the NS5 protein
    T Cui
    Institute of Molecular and Cell Biology, National University of Singapore, Republic of Singapore
    Virology 246:409-17. 1998
    ..These data suggest that the NTPase activity of the NS3 protein may be regulated by the viral NS5 protein during virus replication...
  83. ncbi Kinetics of inhibition of rabbit reticulocyte peptidyltransferase by anisomycin and sparsomycin
    M Ioannou
    Laboratory of Biochemistry, School of Medicine, University of Patras, 26110 Patras, Greece
    Mol Pharmacol 53:1089-96. 1998
    ..During this phase, AcPhe-puromycin is produced from a new, modified ribosomal complex with a lower catalytic rate constant. Thus, sparsomycin also acts as a modifier of eukaryotic peptidyltransferase activity...
  84. pmc The existence of eukaryotic ribonucleoprotein consensus sequence-type RNA-binding proteins in a prokaryote, Synechococcus 6301
    M Sugita
    Center for Gene Research, Nagoya University, Japan
    Nucleic Acids Res 22:25-31. 1994
    ..Due to the easiness of genetic manipulations the cyanobacterium will provide an excellent system to analyze the function of not only cyanobacterial but also plant RNA-binding proteins...
  85. ncbi The A26G replacement in the consensus sequence A-X-X-X-X-G-K-[T,S] of the guanine nucleotide binding site activates the intrinsic GTPase of the elongation factor 2 from the archaeon Sulfolobus solfataricus
    E De Vendittis
    Dipartimento dei Biochimica e Biotecnologie Mediche, , II, Italy
    Eur J Biochem 262:600-5. 1999
    ..These findings suggest that A26 is a regulator of the biochemical functions of SsEF-2. The involvement of this alanine residue in the guanine nucleotide-binding pocket of EF-2 or EF-G is discussed...
  86. ncbi Small molecule-RNA interaction: spectroscopic and calorimetric studies on the binding by the cytotoxic protoberberine alkaloid coralyne to single stranded polyribonucleotides
    Maidul Islam
    Biophysical Chemistry Laboratory, Indian Institute of Chemical Biology, Council of Scientific and Industrial Research, 4, Raja S C Mullick Road, Kolkata 700032, India
    Biochim Biophys Acta 1790:829-39. 2009
    ..This study is aimed to elucidate the specificity of the alkaloid coralyne to poly(G), poly(C), poly(I) and poly(U) in the light of its ability in inducing self-structure in poly(A)...
  87. ncbi Polyadenylic: polyuridylic acid-induced determinants of host resistance to cytomegalovirus and their potentiation by hyperthermia
    B K Lee
    Department of Medical Microbiology and Immunology, University of Minnesota, Duluth 55812 2487
    J Immunother (1991) 12:105-14. 1992
    The ability of spleen cells from poly A:poly U-treated mice to inhibit murine cytomegalovirus (MCMV) replication in confluent monolayer cells of secondary mouse embryo fibroblasts (MEFs) cultured at 37 and 40 degrees C was investigated...
  88. ncbi A distinctive ribonuclease from fresh fruiting bodies of the medicinal mushroom Ganoderma lucidum
    H X Wang
    Department of Microbiology, College of Biological Science, China Agricultural University, Beijing, China
    Biochem Biophys Res Commun 314:519-22. 2004
    ..A pH of 4.0 and a temperature of 60 degrees C were required for optimal activity of the enzyme. The optimum pH was low compared with those reported for other mushroom ribonucleases...
  89. ncbi Altered degradation of circulating nucleic acids and oligonucleotides in diabetic patients
    G Kocic
    Institute of Biochemistry, Medical Faculty University of Nis, Serbia
    Diabetes Res Clin Pract 79:204-13. 2008
    ..Decreased RNase activity and related increase of circulating oligonucleotides may favor the increase of nucleic acid "danger motifs", leading to TLRs activation...
  90. pmc Polyadenylation proteins CstF-64 and tauCstF-64 exhibit differential binding affinities for RNA polymers
    Roberto R Monarez
    Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, 3601 4th Street, Lubbock, TX 79430, USA
    Biochem J 401:651-8. 2007
    ..This supports the hypothesis that tauCstF-64 promotes germ-cell-specific patterns of polyadenylation by binding to different downstream sequence elements...
  91. ncbi RNA synthetic activity of glutamate dehydrogenase: determination of enzyme purity, RNA characteristics, and deamination/amination ratio
    Godson O Osuji
    Cooperative Agricultural Research Center, Prairie View A and M University, Texas A and M University System, PO Box 4079, Prairie View, TX 77446, USA
    Appl Biochem Biotechnol 119:209-28. 2004
    ....
  92. ncbi The formation of phosphate end groups in the radiolysis of polynucleotides in aqueous solution
    C P Murthy
    , Bundesrepublik Deutschland
    Z Naturforsch C 43:572-6. 1988
    ..This is possibly linked to the decomposition of unstable hydroperoxides which are formed in high yields in poly(U) and poly(C) (G = 0.7 and 0.19 mumol J-1, resp.).(ABSTRACT TRUNCATED AT 250 WORDS)..
  93. ncbi A 20-amino-acid autonomous RNA-binding domain contained in an enoyl-CoA hydratase
    J Nakagawa
    Institute for Medical Microbiology, University of Basel, Switzerland
    Eur J Biochem 244:890-9. 1997
    ..By contrast, the hydratase activity of AUH required an intact three-dimensional conformation, as most mutations downstream of Ser68 impaired enzymatic activity...
  94. ncbi Kinetic studies on the interaction between a ribosomal complex active in peptide bond formation and the macrolide antibiotics tylosin and erythromycin
    G P Dinos
    Laboratory of Biochemistry, School of Medicine, University of Patras, 26500 Patras, Greece
    Biochemistry 39:11621-8. 2000
    ..The determination of K(er), k(6), and k(7) enables us to classify erythromycin as a slow-binding ligand of ribosomes...
  95. pmc Characterization of the E.coli poly(A) polymerase: nucleotide specificity, RNA-binding affinities and RNA structure dependence
    S Yehudai-Resheff
    Department of Biology, Technion Israel Institute of Technology, Haifa 32000, Israel
    Nucleic Acids Res 28:1139-44. 2000
    ..However, RNA 3'-ends produced by endonucleolytic cleavage by RNase E in single-strand regions of mRNA molecules may serve as efficient substrates for polyadenylation that direct these molecules for rapid exonucleolytic degradation...
  96. pmc Biochemical characterization of the equine arteritis virus helicase suggests a close functional relationship between arterivirus and coronavirus helicases
    A Seybert
    Institute of Virology and Immunology, University of Wurzburg, Wurzburg, Germany
    J Virol 74:9586-93. 2000
    ..Thus, the combined data strongly support the idea that nidovirus helicases may represent a separate group of RNA virus-encoded helicases with distinct properties...
  97. pmc Changes in ribosomal activity of Escherichia coli cells during prolonged culture in sea salts medium
    D L Kalpaxis
    Laboratory of Biochemistry, School of Medicine, University of Patras, Greece
    J Bacteriol 180:3114-9. 1998
    ..An analogous effect of reconstitution on the efficiency of ribosomes in binding AcPhe-tRNA was evident not only during the initial phase but throughout the starvation period...
  98. ncbi Polysaccharide--polynucleotide complexes. 2. Complementary polynucleotide mimic behavior of the natural polysaccharide schizophyllan in the macromolecular complex with single-stranded RNA and DNA
    K Sakurai
    Organization and Function, PRESTO and Chemotransfiguration Project, Japan Science and Technology Corporation JST, Kurume Research Center Building, 2432 Aikawa, Kurume, Fukuoka 839 0861, Japan
    Biomacromolecules 2:641-50. 2001
    ..Furthermore, stoichiometric study suggested that the complex structure is a triple helix consisting of two s-SPG and one poly(C) or poly(A) chains...
  99. ncbi The EWS gene, involved in Ewing family of tumors, malignant melanoma of soft parts and desmoplastic small round cell tumors, codes for an RNA binding protein with novel regulatory domains
    T Ohno
    Department of Microbiology and Immunology, Jefferson Cancer Institute, Philadelphia, PA 19107 5541
    Oncogene 9:3087-97. 1994
    ..The EWS-b protein binds to RNA in vitro and, specifically, to poly G and poly U. The RNA binding activity was localized to the carboxy terminal 86 amino acids, which constitute RGG box...
  100. ncbi Annexin A2 is a novel RNA-binding protein
    Nolan R Filipenko
    Cancer Biology Research Group, Departments of Biochemistry and Molecular Biology and Oncology, University of Calgary, Calgary, Alberta T2N 4N1, Canada
    J Biol Chem 279:8723-31. 2004
    ..Transfection of LNCaP cells with the ANXA2 gene resulted in the up-regulation of c-Myc protein. These findings identify ANXA2 as a Ca(2+)-dependent RNA-binding protein that interacts with the mRNA of the nuclear oncogene, c-myc...
  101. pmc De novo initiation of RNA synthesis by the RNA-dependent RNA polymerase (NS5B) of hepatitis C virus
    G Luo
    Department of Virology, Bristol Myers Squibb Pharmaceutical Research Institute, Wallingford, Connecticut 06492 7660, USA
    J Virol 74:851-63. 2000
    ..These findings suggest that HCV NS5B is able to initiate RNA synthesis de novo...

Research Grants72

  1. NUCLEOSIDE, NUCLEOTIDE, AND POLYNUCLEOTIDE ANALOGS
    Donald Bergstrom; Fiscal Year: 1980
    ..New synthetic routes to modified nucleosides structurally resembling coformycin will be investigated. Routes for expansion of the heterocyclic rings of 2'-deoxyuridine and inosine are to be examined. ..
  2. ESTROGEN ACTION IN XENOPUS LIVER
    Daniel Schoenberg; Fiscal Year: 1990
    ..Future studies will utilize DNA-mediated gene transfer into cultured hepatocytes as a functional assay for any specific sequences identified as components in the regulation of albumin gene expression...
  3. Cis-acting RNA elements in hepatitis C virus replication
    Guangxiang Luo; Fiscal Year: 2003
    ..abstract_text> ..
  4. Cis-acting RNA elements in hepatitis C virus replication
    Guangxiang Luo; Fiscal Year: 2007
    ..abstract_text> ..
  5. Cis-acting RNA elements in hepatitis C virus replication
    Guangxiang Luo; Fiscal Year: 2006
    ..abstract_text> ..
  6. EXPRESSION OF CYTOCHROME P448 ENVIRONMENTAL INFLUENCES
    Edward Bresnick; Fiscal Year: 1980
    ..Toward these ends, liver messenger RNA fractions will be isolated from drug-treated rats by Sepharose 4B and poly U-Sepharose chromatography and will be tested for translational activity in a wheat germ translational system...
  7. MECHANISMS OF CONTROL OF THE GAP-43 GENE
    Nora Perrone Bizzozero; Fiscal Year: 1999
    ....
  8. MECHANISMS OF CONTROL OF THE GAP-43 GENE
    Nora Perrone Bizzozero; Fiscal Year: 1999
    ....
  9. PROCESSING OF EUKARYOTIC MESSENGER RNA
    Mary Edmonds; Fiscal Year: 1990
    ..Such proteins would be identified initially by their binding to poly U dated to cytoplasmic extracts...
  10. CONTROL OF TRANSCRIPT ELONGATION BY MAMMALIAN RNA POL II
    MICHAEL IZBAN; Fiscal Year: 1999
    ..A crude nuclear fraction termed TFIIX contains a unique cleavage activity. This activity will be further characterized and the protein(s) responsible purified. ..
  11. ANALYSIS OF A MORPHOGENETIC DETERMINANT IN AN EMBRYO
    KLAUS KALTHOFF; Fiscal Year: 1980
    ..We will compare the proteins synthesized in anterior versus posterior egg halves, in normal and UV irradiated eggs, with only maternal or both maternal and embryonic messenger RNA as templates...
  12. EFFECTS OF ELECTROPHILIC ATTACK ON POLYNUCLEOTIDES
    DAVID MCMILLIN; Fiscal Year: 1980
    ..Finally, the utility of Raman difference spectroscopy in supressing troublesome fluorescence backgrounds in other biological samples will be examined...
  13. GENETIC CONTROL OF RIBOSOME ASSEMBLY
    PAUL SYPHERD; Fiscal Year: 1980
    ..Such "50" particles are inactive in a poly U-dependent, poly phenylalanine synthesizing reaction and are inactive in the peptidyl transferase reaction carried ..
  14. MOLECULAR RULERS FOR RIBOSOMES
    Eric Wickstrom; Fiscal Year: 1980
    ..Fragments which are labeled due to alkylation by labeled molecular rulers may be identified by amino acid compositions, which will be determined by HPLC of fluorescently modified amino acids following hydrolysis...
  15. ANTIBODY MODEL FOR RECOGNITION OF DNA HELICAL DIVERSITY
    BERNARD STOLLAR; Fiscal Year: 1990
    ..The aims will be to test hypotheses about the oligonucleotide-protein interacting sites, and to modify the specificity and affinity of the antibodies...
  16. BIOCHEMICAL CHARACTERIZATION OF MESSENGER RNA
    Joe Johnson; Fiscal Year: 1980
    ..They will then be incorporated into synthetic mRNA with the aid of T4RNA ligase. Conformation of all synthetic 'cap' analogs will be analyzed through 3IP-NMR analysis, molecular orbital calculations and Laser Raman analysis. ..
  17. DEVELOPMENTALLY REGULATED MRNAS IN DICTYOSTELIUM
    CARL PALATNIK; Fiscal Year: 1980
    ..Experiments in this proposal will also result in an evaluation of the potential use of poly(U)-Sepharose as a purification step in the isolation of developmentally regulated mRNAs. ..
  18. PROOFREADING OF THE CODON-ANTICODON INTERACTION
    Robert Thompson; Fiscal Year: 1980
    ..coli. defective in protein biosynthesis. The reaction of poly U-programmed ribosomes with Leu-tRNA2 Leu ternary complex is being studied in the pre-steady state phase with a view ..
  19. THE ROLE OF MODIFIED NUCLEOTIDES IN MAMMALIAN TRNA
    Bruce Roe; Fiscal Year: 1980
    ..two mammalian in vitro cell free protein synthesizing systems and have shown that the rT effect we observed in the poly U-directed system can also be observed with a natural m-RNA (i.e. hemoglobin)...
  20. HCV Genotype 6: A Model for HCV Genetics and Molecular Epidemiology Studies
    Ling Lu; Fiscal Year: 2010
    ..This information and the derived models are vital for better strategies of HCV diagnosis and treatment, and the improved designs of antivirals and vaccines. ..
  21. The Nuclear Pore Complex: Interphase and Mitotic Function
    Katharine S Ullman; Fiscal Year: 2010
    ..Such defects in morphology and DNA content are hallmarks of aggressive cancer cells, and the research proposed here will contribute to a better understanding of how these alterations arise. ..
  22. RNA Uridylytransferases of Trypanosomes
    Ruslan Aphasizhev; Fiscal Year: 2007
    ..Sequences of the newly identified UTP-specific transferases will be used to establish characteristic protein motifs for this family of enzymes. ..
  23. Function of 3'UTRs
    MARVIN P WICKENS; Fiscal Year: 2011
    ..These proteins control stem cells and participate in the formation of memory. Understanding how they work should provide practical opportunities for intervention. ..
  24. CLEAVAGE, POLYADENYLATION, AND TRANSPORT OF MRNA
    Marvin Wickens; Fiscal Year: 2007
    ..In focusing sharply on a few specific examples, we hope to illuminate the broad molecular questions of how 3'UTR controls function, evolve, and coordinate exoression of multiole mRNAs. ..
  25. Function of 3' UTRs
    Marvin Wickens; Fiscal Year: 2007
    ..We focus sharply on PUF proteins to illuminate broadly how 3'UTR controls function, evolve, and coordinate expression of multiple mRNAs. ..
  26. The Nuclear Pore Complex: Interphase and Mitotic Function
    KATHARINE ULLMAN; Fiscal Year: 2005
    ..The information gathered will impact practical applications, such as optimizing expression of exogenously introduced genes as well as understanding -and ultimately controlling-growth deregulation found in cancerous cells. ..
  27. The Role of HuR in mutant SOD1 dysregulation of VEGF mRNA Processing
    Peter H King; Fiscal Year: 2010
    ..There is no cure for this disease or any mitigating therapy. This proposal will address a novel area of growth factor regulation that may contribute to the cause of this disease, and thus may ultimately lead to novel therapies. ..
  28. Function of 3' UTRs
    Marvin Wickens; Fiscal Year: 2006
    ..We focus sharply on PUF proteins to illuminate broadly how 3'UTR controls function, evolve, and coordinate expression of multiple mRNAs. ..
  29. Aberrant VEGF RNA Stability in Amyotrophic Lateral Sclerosis
    Peter King; Fiscal Year: 2007
    ..unreadable] [unreadable]..
  30. The Nuclear Pore Complex: Interphase and Mitotic Function
    KATHARINE ULLMAN; Fiscal Year: 2007
    ..The information gathered will impact practical applications, such as optimizing expression of exogenously introduced genes as well as understanding -and ultimately controlling-growth deregulation found in cancerous cells. ..
  31. Function of 3'UTRs
    Marvin Wickens; Fiscal Year: 2009
    ..These proteins control stem cells and participate in the formation of memory. Understanding how they work should provide practical opportunities for intervention. ..
  32. The Role of HuR in mutant SOD1 dysregulation of VEGF mRNA Processing
    Peter H King; Fiscal Year: 2010
    ..There is no cure for this disease or any mitigating therapy. This proposal will address a novel area of growth factor regulation that may contribute to the cause of this disease, and thus may ultimately lead to novel therapies. ..
  33. Function of 3'UTRs
    MARVIN P WICKENS; Fiscal Year: 2010
    ..These proteins control stem cells and participate in the formation of memory. Understanding how they work should provide practical opportunities for intervention. ..
  34. The Nuclear Pore Complex: Interphase and Mitotic Function
    KATHARINE ULLMAN; Fiscal Year: 2009
    ..Such defects in morphology and DNA content are hallmarks of aggressive cancer cells, and the research proposed here will contribute to a better understanding of how these alterations arise. ..
  35. Function of 3'UTRs
    Marvin Wickens; Fiscal Year: 2009
    ..These proteins control stem cells and participate in the formation of memory. Understanding how they work should provide practical opportunities for intervention. ..
  36. The Role of HuR in mutant SOD1 dysregulation of VEGF mRNA Processing
    Peter King; Fiscal Year: 2008
    ..There is no cure for this disease or any mitigating therapy. This proposal will address a novel area of growth factor regulation that may contribute to the cause of this disease, and thus may ultimately lead to novel therapies. ..
  37. Mitochondrial poly(A) Polymerases of Trypanosomes
    Ruslan Aphasizhev; Fiscal Year: 2008
    ..However, a detailed molecular analysis of the polyadenylation process is required for validation of this enzyme as a drug target. ..
  38. The Nuclear Pore Complex: Interphase and Mitotic Function
    KATHARINE ULLMAN; Fiscal Year: 2008
    ..The information gathered will impact practical applications, such as optimizing expression of exogenously introduced genes as well as understanding -and ultimately controlling-growth deregulation found in cancerous cells. ..
  39. The Nuclear Pore Complex: Interphase and Mitotic Function
    KATHARINE ULLMAN; Fiscal Year: 2005
    ..The information gathered will impact practical applications, such as optimizing expression of exogenously introduced genes as well as understanding -and ultimately controlling-growth deregulation found in cancerous cells. ..
  40. Regulation of Lung Epithelial Fibrinolysis by Urokinase
    Sreerama Shetty; Fiscal Year: 2005
    ..This information could hasten the development of new, mechanism-based interventions for lung disorders, including acute lung injury or lung neoplasia. ..
  41. FUNCTION OF 3 UTRS
    Marvin Wickens; Fiscal Year: 2002
    ..Our detailed investigations of the regulation of c-mos, a proto-oncogene normally expressed only in the germ line, bear on how cell division is controlled both in normal and abnormal growth. ..
  42. CONTROL OF FIBRINOLYTIC PATHWAYS IN LUNG DISEASE
    Sreerama Shetty; Fiscal Year: 2001
    ..This work may suggest new therapeutic approaches to a variety of inflammatory or neoplastic lung diseases for which current therapy is often unsatisfactory. ..
  43. Function of 3' UTRs
    Marvin Wickens; Fiscal Year: 2004
    ..We focus sharply on PUF proteins to illuminate broadly how 3'UTR controls function, evolve, and coordinate expression of multiple mRNAs. ..
  44. STUDIES ON B2-ADRENERGIC RECEPTOR MRNA BINDING PROTEIN
    BABY THOLANIKUNNEL; Fiscal Year: 2001
    ..2) To study the mechanisms by which betaAR5B protein participates in beta2AR mRNA degradation and characterize the intracellular properties of this polypeptide. ..
  45. FUNCTION OF 3 UTRS
    Marvin Wickens; Fiscal Year: 2001
    ..Our detailed investigations of the regulation of c-mos, a proto-oncogene normally expressed only in the germ line, bear on how cell division is controlled both in normal and abnormal growth. ..
  46. CONTROL OF FIBRINOLYTIC PATHWAYS IN LUNG DISEASE
    Sreerama Shetty; Fiscal Year: 2000
    ..This work may suggest new therapeutic approaches to a variety of inflammatory or neoplastic lung diseases for which current therapy is often unsatisfactory. ..
  47. FUNCTION OF 3 UTRS
    Marvin Wickens; Fiscal Year: 2000
    ..Our detailed investigations of the regulation of c-mos, a proto-oncogene normally expressed only in the germ line, bear on how cell division is controlled both in normal and abnormal growth. ..
  48. STUDIES ON B2-ADRENERGIC RECEPTOR MRNA BINDING PROTEIN
    BABY THOLANIKUNNEL; Fiscal Year: 2002
    ..2) To study the mechanisms by which betaAR5B protein participates in beta2AR mRNA degradation and characterize the intracellular properties of this polypeptide. ..
  49. MOLECULAR MECHANISMS OF NUCLEAR EXPORT
    KATHARINE ULLMAN; Fiscal Year: 2002
    ..Through genetic and biochemical interaction analyses, Nup153 will be used to construct a bigger picture of key connections made between export machinery components. ..
  50. RNA Uridylytransferases of Trypanosomes
    Ruslan Aphasizhev; Fiscal Year: 2005
    ..Sequences of the newly identified UTP-specific transferases will be used to establish characteristic protein motifs for this family of enzymes. ..
  51. Function of 3' UTRs
    Marvin Wickens; Fiscal Year: 2005
    ..We focus sharply on PUF proteins to illuminate broadly how 3'UTR controls function, evolve, and coordinate expression of multiple mRNAs. ..
  52. MOLECULAR MECHANISMS OF NUCLEAR EXPORT
    KATHARINE ULLMAN; Fiscal Year: 2004
    ..Through genetic and biochemical interaction analyses, Nup153 will be used to construct a bigger picture of key connections made between export machinery components. ..
  53. The Role of HuR in mutant SOD1 dysregulation of VEGF mRNA Processing
    Peter King; Fiscal Year: 2009
    ..There is no cure for this disease or any mitigating therapy. This proposal will address a novel area of growth factor regulation that may contribute to the cause of this disease, and thus may ultimately lead to novel therapies. ..
  54. STUDIES ON B2-ADRENERGIC RECEPTOR MRNA BINDING PROTEIN
    BABY THOLANIKUNNEL; Fiscal Year: 2003
    ..2) To study the mechanisms by which betaAR5B protein participates in beta2AR mRNA degradation and characterize the intracellular properties of this polypeptide. ..
  55. CONTROL OF FIBRINOLYTIC PATHWAYS IN LUNG DISEASE
    Sreerama Shetty; Fiscal Year: 2002
    ..This work may suggest new therapeutic approaches to a variety of inflammatory or neoplastic lung diseases for which current therapy is often unsatisfactory. ..
  56. RNA Uridylytransferases of Trypanosomes
    Ruslan Aphasizhev; Fiscal Year: 2009
    ..Sequences of the newly identified UTP-specific transferases will be used to establish characteristic protein motifs for this family of enzymes. ..
  57. Structures and Functions of RNA Editing TUTases
    Ruslan Aphasizhev; Fiscal Year: 2011
    ..Targeting essential parasite-specific enzymes, such as mitochondrial RNA editing terminal uridylyl transferases (TUTases), is a promising approach toward a new generation of trypanocides. ..
  58. RNA Uridylytransferases of Trypanosomes
    Ruslan Aphasizhev; Fiscal Year: 2008
    ..Sequences of the newly identified UTP-specific transferases will be used to establish characteristic protein motifs for this family of enzymes. ..
  59. FUNCTION OF 3 UTRS
    Marvin Wickens; Fiscal Year: 1999
    ..Our detailed investigations of the regulation of c-mos, a proto-oncogene normally expressed only in the germ line, bear on how cell division is controlled both in normal and abnormal growth. ..
  60. RNA Uridylytransferases of Trypanosomes
    Ruslan Aphasizhev; Fiscal Year: 2006
    ..Sequences of the newly identified UTP-specific transferases will be used to establish characteristic protein motifs for this family of enzymes. ..
  61. MOLECULAR MECHANISMS OF NUCLEAR EXPORT
    KATHARINE ULLMAN; Fiscal Year: 2003
    ..Through genetic and biochemical interaction analyses, Nup153 will be used to construct a bigger picture of key connections made between export machinery components. ..
  62. MOLECULAR MECHANISMS OF NUCLEAR EXPORT
    KATHARINE ULLMAN; Fiscal Year: 2001
    ..Through genetic and biochemical interaction analyses, Nup153 will be used to construct a bigger picture of key connections made between export machinery components. ..
  63. CLEAVAGE, POLYADENYLATION, AND TRANSPORT OF MRNA
    Marvin Wickens; Fiscal Year: 2008
    ..In focusing sharply on the GLD-2 family, and a few selected biological contexts, we hope to address the broad questions of how 3'UTR controls function, evolve, and coordinate expression of multiple mRNAs. ..
  64. Aberrant VEGF RNA Stability in Amyotrophic Lateral Sclerosis
    Peter King; Fiscal Year: 2008
    ..unreadable] [unreadable]..
  65. The Nuclear Pore Complex: Interphase and Mitotic Function
    KATHARINE ULLMAN; Fiscal Year: 2006
    ..The information gathered will impact practical applications, such as optimizing expression of exogenously introduced genes as well as understanding -and ultimately controlling-growth deregulation found in cancerous cells. ..