adenosine diphosphate ribose

Summary

Summary: An ester formed between the aldehydic carbon of RIBOSE and the terminal phosphate of ADENOSINE DIPHOSPHATE. It is produced by the hydrolysis of nicotinamide-adenine dinucleotide (NAD) by a variety of enzymes, some of which transfer an ADP-ribosyl group to target proteins.

Top Publications

  1. ncbi Mouse Sir2 homolog SIRT6 is a nuclear ADP-ribosyltransferase
    Gregory Liszt
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Biol Chem 280:21313-20. 2005
  2. ncbi TRPM2: a calcium influx pathway regulated by oxidative stress and the novel second messenger ADP-ribose
    Frank J P Kühn
    Medical Faculty, RWTH Aachen, Institute of Physiology, Pauwelsstrasse 30, 52057 Aachen, Germany
    Pflugers Arch 451:212-9. 2005
  3. ncbi Haploid genetic screens in human cells identify host factors used by pathogens
    Jan E Carette
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA
    Science 326:1231-5. 2009
  4. pmc The macro domain is an ADP-ribose binding module
    Georgios I Karras
    Gene Expression Programme and Structural and Computational Biology Programme, European Molecular Biology Laboratory, Heidelberg, Germany
    EMBO J 24:1911-20. 2005
  5. ncbi ADP-ribose gating of the calcium-permeable LTRPC2 channel revealed by Nudix motif homology
    A L Perraud
    Department of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA
    Nature 411:595-9. 2001
  6. doi Transient receptor potential melastatin 2 is required for lipopolysaccharide-induced cytokine production in human monocytes
    Janine Wehrhahn
    Department of Immunobiology, Institute of Biology II, University of Leipzig, 04277 Leipzig, Germany
    J Immunol 184:2386-93. 2010
  7. ncbi A biochemical genomics approach for identifying genes by the activity of their products
    M R Martzen
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, Rochester, NY 14642, USA
    Science 286:1153-5. 1999
  8. pmc Structure and biochemical functions of SIRT6
    Patricia W Pan
    Structural Genomics Consortium, University of Toronto, Toronto, Ontario M5G 1L7, Canada
    J Biol Chem 286:14575-87. 2011
  9. ncbi Hydrogen peroxide and ADP-ribose induce TRPM2-mediated calcium influx and cation currents in microglia
    Robert Kraft
    Institut fur Pharmakologie, Charite Universitatsmedizin Berlin, Campus Benjamin Franklin, Thielallee 69 73, 14195 Berlin, Germany
    Am J Physiol Cell Physiol 286:C129-37. 2004
  10. pmc A role for poly(ADP-ribose) polymerase in the transcriptional regulation of the melanoma growth stimulatory activity (CXCL1) gene expression
    C Nirodi
    Department of Cell Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Biol Chem 276:9366-74. 2001

Detail Information

Publications261 found, 100 shown here

  1. ncbi Mouse Sir2 homolog SIRT6 is a nuclear ADP-ribosyltransferase
    Gregory Liszt
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Biol Chem 280:21313-20. 2005
    ..These results provide the first characterization of a Sir2 protein from phylogenetic class IV...
  2. ncbi TRPM2: a calcium influx pathway regulated by oxidative stress and the novel second messenger ADP-ribose
    Frank J P Kühn
    Medical Faculty, RWTH Aachen, Institute of Physiology, Pauwelsstrasse 30, 52057 Aachen, Germany
    Pflugers Arch 451:212-9. 2005
    ..Moreover, it plays a decisive role in experimentally induced diabetes mellitus and in the activation of leukocytes...
  3. ncbi Haploid genetic screens in human cells identify host factors used by pathogens
    Jan E Carette
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA
    Science 326:1231-5. 2009
    ..This approach has both conceptual and practical parallels with genetic approaches in haploid yeast...
  4. pmc The macro domain is an ADP-ribose binding module
    Georgios I Karras
    Gene Expression Programme and Structural and Computational Biology Programme, European Molecular Biology Laboratory, Heidelberg, Germany
    EMBO J 24:1911-20. 2005
    ..In addition, some macro domain proteins also recognize poly-ADP-ribose as a ligand. Our data suggest an important role for proteins containing macro domains in the biology of ADP-ribose...
  5. ncbi ADP-ribose gating of the calcium-permeable LTRPC2 channel revealed by Nudix motif homology
    A L Perraud
    Department of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA
    Nature 411:595-9. 2001
    ..These results indicate that intracellular ADPR regulates calcium entry into cells that express LTRPC2...
  6. doi Transient receptor potential melastatin 2 is required for lipopolysaccharide-induced cytokine production in human monocytes
    Janine Wehrhahn
    Department of Immunobiology, Institute of Biology II, University of Leipzig, 04277 Leipzig, Germany
    J Immunol 184:2386-93. 2010
    ..The identification of TRPM2 as a major player in this LPS-dependent process makes it a promising tool in modulating monocyte functions...
  7. ncbi A biochemical genomics approach for identifying genes by the activity of their products
    M R Martzen
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, Rochester, NY 14642, USA
    Science 286:1153-5. 1999
    ....
  8. pmc Structure and biochemical functions of SIRT6
    Patricia W Pan
    Structural Genomics Consortium, University of Toronto, Toronto, Ontario M5G 1L7, Canada
    J Biol Chem 286:14575-87. 2011
    ..Collectively, these new insights imply a unique activating mechanism and/or the possibility that SIRT6 could act as an NAD(+) metabolite sensor...
  9. ncbi Hydrogen peroxide and ADP-ribose induce TRPM2-mediated calcium influx and cation currents in microglia
    Robert Kraft
    Institut fur Pharmakologie, Charite Universitatsmedizin Berlin, Campus Benjamin Franklin, Thielallee 69 73, 14195 Berlin, Germany
    Am J Physiol Cell Physiol 286:C129-37. 2004
    ..The increased sensitivity to H2O2 in LPS-stimulated cells suggests a role for TRPM2 in the calcium signaling of activated microglia...
  10. pmc A role for poly(ADP-ribose) polymerase in the transcriptional regulation of the melanoma growth stimulatory activity (CXCL1) gene expression
    C Nirodi
    Department of Cell Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Biol Chem 276:9366-74. 2001
    ..Furthermore, 3-aminobenzamide, an inhibitor of PARP-specific ADP-ribosylation, inhibits CXCL1 promoter activity and reduces levels of CXCL1 mRNA. The data point to the possibility that PARP may be a coactivator of CXCL1 transcription...
  11. doi Hydrolysis of the phosphoanhydride linkage of cyclic ADP-ribose by the Mn(2+)-dependent ADP-ribose/CDP-alcohol pyrophosphatase
    José Canales
    Grupo de Enzimologia, Departamento de Bioquimica y Biologia Molecular y Genetica, Facultad de Medicina, Universidad de Extremadura, Badajoz, Spain
    FEBS Lett 583:1593-8. 2009
    ..This is similar to the ADP-ribose/cADPR formation ratio by NADases. The product of cADPR phosphohydrolysis by ADPRibase-Mn was N(1)-(5-phosphoribosyl)-AMP, suggesting a novel route for cADPR turnover...
  12. pmc TRPM2 functions as a lysosomal Ca2+-release channel in beta cells
    Ingo Lange
    Center for Biomedical Research, The Queen s Medical Center, Honolulu, HI 96813, USA
    Sci Signal 2:ra23. 2009
    ..Thus, ADPR and TRPM2 represent multimodal signaling elements regulating Ca2+ mobilization in beta cells through membrane depolarization, Ca2+ influx, and release of Ca2+ from intracellular stores...
  13. ncbi Transient ADP-ribosylation of a 2'-phosphate implicated in its removal from ligated tRNA during splicing in yeast
    S L Spinelli
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, Rochester, New York 14642, USA
    J Biol Chem 274:2637-44. 1999
    ..Step 1 of this reaction is strikingly similar to the ADP-ribosylation of proteins catalyzed by a number of bacterial toxins...
  14. ncbi Regulation of calcium signalling in T lymphocytes by the second messenger cyclic ADP-ribose
    A H Guse
    Department of Enzyme Chemistry, Institute of Physiological Chemistry, University of Hamburg, Germany
    Nature 398:70-3. 1999
    ..Thus we show the presence, causal relation and biological significance of the major constituents of the cADPR/calcium-signalling pathway in human T cells...
  15. pmc Combining affinity purification by ADP-ribose-binding macro domains with mass spectrometry to define the mammalian ADP-ribosyl proteome
    Nadia Dani
    Department of Cell Biology and Oncology, Consorzio Mario Negri Sud, Via Nazionale, 66030 Santa Maria Imbaro Chieti, Italy
    Proc Natl Acad Sci U S A 106:4243-8. 2009
    ..This represents an important step toward the discovery of new ADP-ribosyltransferase targets and an understanding of the physiological role and the pharmacological potential of this protein modification...
  16. ncbi A new detection method for arginine-specific ADP-ribosylation of protein -- a combinational use of anti-ADP-ribosylarginine antibody and ADP-ribosylarginine hydrolase
    Harumi Osago
    Department of Biochemistry, Faculty of Medicine, Shimane University, Izumo 693 8501, Japan
    J Biochem Biophys Methods 70:1014-9. 2008
    ..We applied the method to search endogenously ADP-ribosylated protein in the rat, and detected possible target proteins in the skeletal muscle, which has high Art activity...
  17. ncbi Botulinum C2 toxin ADP-ribosylates cytoplasmic beta/gamma-actin in arginine 177
    J Vandekerckhove
    Laboratorium voor Genetika, Gent, Belgium
    J Biol Chem 263:696-700. 1988
    ..Further digestion of these two peptides with thermolysin yielded the same radioactive peptide, which was in both cases peptide 175-177. Amino acid sequence analysis of peptides 174-183 and 175-177 located the ADP-ribosylation on Arg177...
  18. ncbi A proposed mechanism of ADP-ribosylation catalyzed by the pertussis toxin S1 subunit
    C Locht
    Laboratoire de Microbiologie Génétique et Moléculaire, INSERM CJF 9109, Institut Pasteur de Lille, France
    Biochimie 77:333-40. 1995
    ..His-35 could increase the nucleophilicity of the cysteine in the G protein or the water molecule to attack the weakened N-glycosidic bond of NAD+ and yield the products of the reaction...
  19. pmc ADP-ribosylation of human defensin HNP-1 results in the replacement of the modified arginine with the noncoded amino acid ornithine
    Linda A Stevens
    Translational Medicine Branch and Laboratory of Biochemistry, National Heart, Lung and Blood Institute, Medical Genetics Branch, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 106:19796-800. 2009
    ..Targeted ADP-ribosylation of specific arginines by transferases, resulting in their replacement with ornithine, is an alternative pathway for regulation of protein function through posttranslational modification...
  20. ncbi Metabolite of SIR2 reaction modulates TRPM2 ion channel
    Olivera Grubisha
    Department of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, Oregon 97239, USA
    J Biol Chem 281:14057-65. 2006
    ..ADP-ribose binds TRPM2 with similarly affinity, whereas NAD+ displays almost negligible binding. These studies provide the first evidence for the potential role of sirtuin-generated OAADPr in TRPM2 channel gating...
  21. pmc TRPM2 channel opening in response to oxidative stress is dependent on activation of poly(ADP-ribose) polymerase
    Elena Fonfria
    Neurology and GI Centre of Excellence for Drug Discovery, GlaxoSmithKline Research and Development Limited, New Frontiers Science Park, Third Avenue, Harlow, Essex CM19 5AW
    Br J Pharmacol 143:186-92. 2004
    ..64+/-0.38; 6.68+/-0.28; 4.78+/-0.05, respectively). 6. These data suggest that oxidative stress causes TRPM2 channel opening in both recombinant and endogenously expressing cell systems via activation of PARP enzymes...
  22. pmc Inhibition of TRPM2 cation channels by N-(p-amylcinnamoyl)anthranilic acid
    Robert Kraft
    Institut fur Pharmakologie, Charite Campus Benjamin Franklin, Thielallee 69 73, Berlin 14195, Germany
    Br J Pharmacol 148:264-73. 2006
    ..Owing to its high potency and efficacy ACA can serve, in combination with other blockers, as a useful tool for studying the unknown function of TRPM2 in native cells...
  23. pmc Identification of direct and indirect effectors of the transient receptor potential melastatin 2 (TRPM2) cation channel
    Balazs Toth
    Department of Medical Biochemistry, Semmelweis University, Budapest H 1094, Hungary
    J Biol Chem 285:30091-102. 2010
    ..H(2)O(2), cADPR, and NAADP did not enhance activation by ADPR. Considering intracellular concentrations of these compounds, none of them are likely to directly affect the TRPM2 channel protein in a physiological context...
  24. ncbi Extracellular-added ADP-ribose increases intracellular free Ca2+ concentration through Ca2+ release from stores, but not through TRPM2-mediated Ca2+ entry, in rat beta-cell line RIN-5F
    Masakazu Ishii
    Department of Pathophysiology, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan
    J Pharmacol Sci 101:174-8. 2006
    ..H2O2-induced Ca2+ entry through TRPM2 was not affected by extracellular ADP-ribose. These findings suggest that extracellular-added ADP-ribose induces Ca2+ release through the PLC-IP3 pathway and does not act as a TRPM2 activator...
  25. doi Differential activities of cellular and viral macro domain proteins in binding of ADP-ribose metabolites
    Maarit Neuvonen
    Institute of Biotechnology, University of Helsinki, Helsinki, Finland
    J Mol Biol 385:212-25. 2009
    ..The differential activity profiles of the human proteins implicate them in different cellular pathways, some of which may involve RNA rather than ADP-ribose derivatives...
  26. pmc Enhanced sensitivity to cholera toxin in ADP-ribosylarginine hydrolase-deficient mice
    Jiro Kato
    Pulmonary Critical Care Medicine Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Building 10, Room 6D05, MSC 1590, Bethesda, MD 20892 1590, USA
    Mol Cell Biol 27:5534-43. 2007
    ..In disease, toxin-catalyzed ADP-ribosylation overwhelms this potential host defense system, resulting in persistence of ADP-ribosylation and intoxication of the cell...
  27. doi Mn2+-dependent ADP-ribose/CDP-alcohol pyrophosphatase: a novel metallophosphoesterase family preferentially expressed in rodent immune cells
    José Canales
    Grupo de Enzimologia, Departamento de Bioquimica y Biologia Molecular y Genetica, Facultad de Medicina, Universidad de Extremadura, 06080 Badajoz, Spain
    Biochem J 413:103-13. 2008
    ..Furthermore, the influence of ADPRibase-Mn on the CDP-choline or CDP-ethanolamine pathways of phospholipid biosynthesis cannot be discounted...
  28. ncbi Role of TRPM2 channel in mediating H2O2-induced Ca2+ entry and endothelial hyperpermeability
    Claudie M Hecquet
    Department of Pharmacology and Center for Lung and Vascular Biology, University of Illinois College of Medicine, Chicago, IL 60612, USA
    Circ Res 102:347-55. 2008
    ..Thus, TRPM2 mediates H2O2-induced increase in endothelial permeability through the activation of Ca2+ entry via TRPM2. TRPM2 represents a novel therapeutic target directed against oxidant-induced endothelial barrier disruption...
  29. pmc Redox-dependent change of nucleotide affinity to the active site of the mammalian complex I
    Vera G Grivennikova
    Department of Biochemistry, School of Biology, Moscow State University, Moscow 119992, Russian Federation
    Biochemistry 46:10971-8. 2007
    ..Possible models to explain the different titration patterns for the forward and reverse reactions are briefly discussed...
  30. doi A ternary mechanism for NADH oxidation by positively charged electron acceptors, catalyzed at the flavin site in respiratory complex I
    James A Birrell
    Medical Research Council Mitochondrial Biology Unit, Wellcome Trust MRC Building, Cambridge, UK
    FEBS Lett 585:2318-22. 2011
    ..Our mechanism for paraquat reduction defines a new mechanism for superoxide production by complex I (by redox cycling); in contrast to direct O(2) reduction the rate is stimulated, not inhibited, by high NADH concentrations...
  31. ncbi Auto-ADP-ribosylation of Pseudomonas aeruginosa ExoS
    Matthew J Riese
    Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Biol Chem 277:12082-8. 2002
    ..The 50-kDa protein was determined to be auto-ADP-ribosylated ExoS, whereas the 25-kDa protein appeared to represent a group of proteins that included Ras...
  32. doi Expression and functional properties of TRPM2 channels in dopaminergic neurons of the substantia nigra of the rat
    Kenny K H Chung
    Department of Physiology and Centre for Brain Research, Faculty of Medical and Health Sciences, University of Auckland, Auckland, New Zealand
    J Neurophysiol 106:2865-75. 2011
    ..Given the involvement of oxidative stress in degeneration of SNc neurons in Parkinson's disease, further studies are needed to determine the pathophysiological role of these channels in the disease process...
  33. ncbi Activation of the cation channel long transient receptor potential channel 2 (LTRPC2) by hydrogen peroxide. A splice variant reveals a mode of activation independent of ADP-ribose
    Edith Wehage
    Institute of Physiology, Medical Faculty, Rheinisch Westfälische Technische Hochschule Aachen, Pauwelsstrasse 30, D 52057 Aachen, Germany
    J Biol Chem 277:23150-6. 2002
    ..We conclude that activation of LTRPC2 by H(2)O(2) is independent of ADP-ribose and that LTRPC2 may mediate the influx of Na(+) and Ca(2+) during oxidative stress, such as the respiratory burst in granulocytes...
  34. pmc Structure and mechanism of activity of the cyclic phosphodiesterase of Appr>p, a product of the tRNA splicing reaction
    A Hofmann
    Protein Structure Section, Macromolecular Crystallography Laboratory, Program in Structural Biology, NCI Frederick, Frederick, MD 21702, USA
    EMBO J 19:6207-17. 2000
    ....
  35. pmc The structure of human ADP-ribosylhydrolase 3 (ARH3) provides insights into the reversibility of protein ADP-ribosylation
    Christoph Mueller-Dieckmann
    European Molecular Biology Laboratory Hamburg Outstation, c o Deutsches Elektronen Synchrotron, Institute of Immunology, University Hospital, Hamburg, Germany
    Proc Natl Acad Sci U S A 103:15026-31. 2006
    ..Our results underscore the importance of endogenous ADP-ribosylation cycles and provide a basis for structure-based design of ADP-ribosylhydrolase inhibitors...
  36. pmc Modulation of redox homeostasis under suboptimal conditions by Arabidopsis nudix hydrolase 7
    Niranjani Jambunathan
    246 Noble Research Center, Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma, USA
    BMC Plant Biol 10:173. 2010
    ..Secondly, we evaluate the biochemical and molecular consequences of increased NADH levels due to loss of function of AtNUDT7 in Arabidopsis...
  37. doi Molecular mechanism of ADP-ribose hydrolysis by human NUDT5 from structural and kinetic studies
    Manwu Zha
    State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China
    J Mol Biol 379:568-78. 2008
    ..Structural comparison of different ADPRases also suggests that most dimeric ADPRases may share a similar catalytic mechanism of ADPR hydrolysis...
  38. pmc Identification of an iron-sulfur cluster that modulates the enzymatic activity in NarE, a Neisseria meningitidis ADP-ribosyltransferase
    Mariangela Del Vecchio
    Novartis Vaccines and Diagnostics, Via Fiorentina 1, 53100 Siena, Italy
    J Biol Chem 284:33040-7. 2009
    ..This is the first observation suggesting that a member of the ADP-ribosyltransferase family contains an Fe-S cluster implicated in catalysis. This observation may unravel novel functions exerted by this class of enzymes...
  39. pmc Structural basis for different substrate specificities of two ADP-ribose pyrophosphatases from Thermus thermophilus HB8
    Taisuke Wakamatsu
    Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565 0871, Japan
    J Bacteriol 190:1108-17. 2008
    ..These results suggest that ADPRase-I and ADPRase-II enzymes have nearly identical catalytic mechanisms but different mechanisms of substrate recognition...
  40. pmc Inactivation of the elongation factor Tu by mosquitocidal toxin-catalyzed mono-ADP-ribosylation
    Jörg Schirmer
    Institut fur Experimentelle und Klinische Pharmakologie und Toxikologie, Albert Ludwigs Universitat Freiburg, D 79104 Freiburg, Germany
    Appl Environ Microbiol 68:4894-9. 2002
    ..The inactivation of EF-Tu by MTX-mediated ADP-ribosylation and the resulting inhibition of bacterial protein synthesis are likely to play important roles in the cytotoxicity of the 27-kDa enzyme fragment of MTX toward E. coli...
  41. doi Precursor ion scanning and sequencing of arginine-ADP-ribosylated peptide by mass spectrometry
    Harumi Osago
    Department of Biochemistry, Shimane University, Faculty of Medicine, Izumo, Japan
    Anal Biochem 393:248-54. 2009
    ..We propose that a combination of the precursor ion scanning with ADP-R-carbodiimide as a marker ion and two-step collision is useful in searching for physiological target proteins of Arg-ADP-ribosylation...
  42. ncbi The new life of a centenarian: signalling functions of NAD(P)
    Felicitas Berger
    Institut fur Biochemie, Freie Universitat Berlin, Thielallee 63, 14195 Berlin, Germany
    Trends Biochem Sci 29:111-8. 2004
    ..The comprehensive molecular characterization of NAD biosynthetic pathways over the past few years has further extended the understanding of the multiple roles of pyridine nucleotides in cell biology...
  43. pmc Structural and functional basis for ADP-ribose and poly(ADP-ribose) binding by viral macro domains
    Marie Pierre Egloff
    Centre National de la Recherche Scientifique and Universités d Aix Marseille I et II, UMR 6098, Architecture et Fonction des Macromolecules Biologiques, Ecole Supérieure d Ingénieurs de Luminy Case 925, France
    J Virol 80:8493-502. 2006
    ..Collectively, these results suggest to further evaluate the role of viral macro domains in host response to viral infection...
  44. pmc Nuclear ADP-ribosylation reactions in mammalian cells: where are we today and where are we going?
    Paul O Hassa
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    Microbiol Mol Biol Rev 70:789-829. 2006
    ..e., DNA damage response, transcriptional regulation, and insulator function), are described. A potential cross talk between nuclear ADP-ribosylation processes and other NAD(+)-dependent pathways is discussed...
  45. doi Photorhabdus luminescens toxins ADP-ribosylate actin and RhoA to force actin clustering
    Alexander E Lang
    Institut fur Experimentelle und Klinische Pharmakologie und Toxikologie, Albert Ludwigs Universitat Freiburg, 79104 Freiburg, Germany
    Science 327:1139-42. 2010
    ..The concerted action of both toxins inhibited phagocytosis of target insect cells and induced extensive intracellular polymerization and clustering of actin. Several human pathogenic bacteria produce related toxins...
  46. ncbi Activation of T cell calcium influx by the second messenger ADP-ribose
    Andreas Gasser
    University Medical Center Hamburg Eppendorf, Center of Experimental Medicine, Institute of Biochemistry and Molecular Biology I Cellular Signal Transduction
    J Biol Chem 281:2489-96. 2006
    ..Moreover, gene silencing of TRPM2 abolished the ADPR- and ConA-mediated inward current. Thus, ADPR is a novel second messenger significantly involved in ConA-mediated cell death in T cells...
  47. pmc Expression profile of the transient receptor potential (TRP) family in neutrophil granulocytes: evidence for currents through long TRP channel 2 induced by ADP-ribose and NAD
    Inka Heiner
    Institut fur Physiologie, Universitätsklinikum der RWTH Aachen, Pauwelsstr 30, 52074 Aachen, Germany
    Biochem J 371:1045-53. 2003
    ..The results provide evidence that LTRPC2 in neutrophil granulocytes forms an entry pathway for Na(+) and Ca(2+), which is regulated by ADP-ribose and the redox state...
  48. ncbi Structural basis of severe acute respiratory syndrome coronavirus ADP-ribose-1''-phosphate dephosphorylation by a conserved domain of nsP3
    Kumar Singh Saikatendu
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Structure 13:1665-75. 2005
    ..Sequence and structure comparison of all known macro-H2A domains combined with available functional data suggests that proteins of this superfamily form an emerging group of nucleotide phosphatases that dephosphorylate Appr-1''-p...
  49. ncbi Structure and mechanism of MT-ADPRase, a nudix hydrolase from Mycobacterium tuberculosis
    Lin Woo Kang
    Department of Biophysics and Biophysical Chemistry, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA
    Structure 11:1015-23. 2003
    ..0 and 2.3 A, showed that there are sequence differences in binding site residues between MT-ADPRase and a human homolog that may be exploited for antituberculosis drug development...
  50. ncbi Purification and characterization of adenosine diphosphate ribose pyrophosphatase from human erythrocytes
    J S Kim
    Department of Biochemistry, Medical School, Chonbuk National University, Chonju, South Korea
    Int J Biochem Cell Biol 30:629-38. 1998
    ..These results suggest that our enzyme is unique, and different from the other ADP-ribose pyrophosphatases reported. ADP-ribose pyrophosphatase may play an important role in the regulation of intracellular steady-state of free ADP-ribose...
  51. ncbi Mouse T cell membrane proteins Rt6-1 and Rt6-2 are arginine/protein mono(ADPribosyl)transferases and share secondary structure motifs with ADP-ribosylating bacterial toxins
    F Koch-Nolte
    DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, California 94304, USA
    J Biol Chem 271:7686-93. 1996
    ..4.2.31). They are the first such enzymes to be molecularly characterized in the immune system...
  52. ncbi Spatial control of Ca2+ signaling by nicotinic acid adenine dinucleotide phosphate diffusion and gradients
    G C Churchill
    University of Oxford, Department of Pharmacology, Mansfield Road, Oxford OX1 3QT, United Kingdom
    J Biol Chem 275:38687-92. 2000
    ..Thus, the range of action of NAADP can be tuned by its concentration from that of a local messenger, like Ca(2+), to that of a global messenger, like IP(3) or cyclic ADP-ribose...
  53. ncbi Chemistry of gene silencing: the mechanism of NAD+-dependent deacetylation reactions
    A A Sauve
    Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Biochemistry 40:15456-63. 2001
    ..This mechanism is unprecedented in ADP-ribosyl transferase enzymology. The 2'- and 3'-AADPR products are candidate molecules for SIR2-initiated signaling pathways...
  54. ncbi Characterisation of recombinant rat TRPM2 and a TRPM2-like conductance in cultured rat striatal neurones
    Kerstin Hill
    Neurology, GlaxoSmithKline Research and Development Ltd, New Frontiers Science Park North, Harlow, Essex, UK
    Neuropharmacology 50:89-97. 2006
    ....
  55. pmc ADP-ribosylation of integrin alpha7 modulates the binding of integrin alpha7beta1 to laminin
    Zhefeng Zhao
    Department of Biochemistry, Kansas State University, 104 Willard Hall, Manhattan, KS 66506, USA
    Biochem J 385:309-17. 2005
    ....
  56. ncbi Glutamic acid 207 in rodent T-cell RT6 antigens is essential for arginine-specific ADP-ribosylation
    N Hara
    Department of Biochemistry, Shimane Medical University, 89 1, Izumo, Shimane 693, Japan
    J Biol Chem 271:29552-5. 1996
    ..Moreover, the mouse homologue of rat RT6 lost arginine-specific ADP-ribosyltransferase activity when Glu-207 was replaced with Gln. Thus, Glu-207 in rodent T-cell RT6 antigens is essential for transfer reaction of ADP-ribose to arginine...
  57. ncbi Cloning and characterization of a new member of the Nudix hydrolases from human and mouse
    H Yang
    Department of Microbiology and Molecular Genetics and the Molecular Biology Institute, University of California, Los Angeles, California 90095, USA
    J Biol Chem 275:8844-53. 2000
    ..The genomic structures of both NUDT5 and mNudT5 were determined and located on human chromosome 10 and mouse chromosome 2, respectively. The role of NUDT5 in maintaining levels of free ADP-ribose in cells is discussed...
  58. ncbi New functions of a long-known molecule. Emerging roles of NAD in cellular signaling
    M Ziegler
    Freie Universitat Berlin, Institut fur Biochemie, Berlin, Germany
    Eur J Biochem 267:1550-64. 2000
    ..Both cADPR and NAADP+ have been reported to be potent intracellular calcium-mobilizing agents. In concert with inositol 1,4,5-trisphosphate, they participate in cytosolic calcium regulation by releasing calcium from intracellular stores...
  59. ncbi Structural insights into the Thermus thermophilus ADP-ribose pyrophosphatase mechanism via crystal structures with the bound substrate and metal
    Sachiko Yoshiba
    Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560 0043, Japan
    J Biol Chem 279:37163-74. 2004
    ..This water molecule is situated at an ideal position to carry out nucleophilic attack on the adenosyl phosphate, as it is 3.6 A away from the target phosphorus and almost in line with the scissile bond...
  60. pmc Human alpha-defensins neutralize toxins of the mono-ADP-ribosyltransferase family
    Chun Kim
    Department of Immunology, Max Planck Institute for Infection Biology, Schumannstrasse 21 22, D 10117 Berlin, Germany
    Biochem J 399:225-9. 2006
    ..Our results reveal that toxin neutralization represents a novel biological function of HNPs in host defence...
  61. ncbi The mechanism of the elongation and branching reaction of poly(ADP-ribose) polymerase as derived from crystal structures and mutagenesis
    A Ruf
    Institut fur Organische Chemie und Biochemie, Albertstr 21, Freiburg im Breisgau, D 79104, Germany
    J Mol Biol 278:57-65. 1998
    ....
  62. ncbi Crystal structures of human NUDT5 reveal insights into the structural basis of the substrate specificity
    Manwu Zha
    State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
    J Mol Biol 364:1021-33. 2006
    ....
  63. doi Molecular mechanisms of the cytotoxicity of ADP-ribosylating toxins
    Qing Deng
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Annu Rev Microbiol 62:271-88. 2008
    ..Bacterial toxins have been utilized as vaccines, as tools to dissect host cell physiology, and more recently for the development of novel therapies to treat human disease...
  64. ncbi Accumulation of free ADP-ribose from mitochondria mediates oxidative stress-induced gating of TRPM2 cation channels
    Anne Laure Perraud
    Department of Pediatrics, University of Washington and Children s Hospital and Regional Medical Center, 1959 N E Pacific Ave, Seattle, WA 98195, USA
    J Biol Chem 280:6138-48. 2005
    ....
  65. pmc A highly specific phosphatase that acts on ADP-ribose 1''-phosphate, a metabolite of tRNA splicing in Saccharomyces cerevisiae
    Neil P Shull
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine 601 Elmwood Avenue, Rochester, NY 14642, USA
    Nucleic Acids Res 33:650-60. 2005
    ..poa1-Delta mutants have no obvious growth defect at different temperatures in rich media, and analysis of yeast extracts suggests that approximately 90% of Appr1p processing activity originates from Poa1p...
  66. pmc Conserved cysteine residues in the pore region are obligatory for human TRPM2 channel function
    Zhu Zhong Mei
    Institute of Membrane and Systems Biology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK
    Am J Physiol Cell Physiol 291:C1022-8. 2006
    ..These results taken together suggest that the cysteine residues in the pore region are obligatory for TRPM2 channel function...
  67. ncbi Three-dimensional reconstruction using transmission electron microscopy reveals a swollen, bell-shaped structure of transient receptor potential melastatin type 2 cation channel
    Yuusuke Maruyama
    Neuroscience Research Institute, National Institute of Advanced Industrial Science and Technology AIST, Umezono 1 1 4, Tsukuba, Ibaraki 305 8568, Japan
    J Biol Chem 282:36961-70. 2007
    ..2 (TRPM2) is a redox-sensitive, calcium-permeable cation channel activated by various signals, such as adenosine diphosphate ribose (ADPR) acting on the ADPR pyrophosphatase (ADPRase) domain, and cyclic ADPR...
  68. ncbi Comprehensive analysis of cytosolic Nudix hydrolases in Arabidopsis thaliana
    Takahisa Ogawa
    Department of Advanced Bioscience, Faculty of Agriculture, Kinki University, 3327 204 Nakamachi, Nara 631 8505, Japan
    J Biol Chem 280:25277-83. 2005
    ..coli MutT in A. thaliana and is involved in the prevention of spontaneous mutation. The results obtained here suggest that the plant Nudix family has evolved in a specific manner that differs from that of yeast and humans...
  69. ncbi Biosynthesis of diphthamide in Saccharomyces cerevisiae. Partial purification and characterization of a specific S-adenosylmethionine:elongation factor 2 methyltransferase
    J Y Chen
    Department of Biochemistry, University of Minnesota, Minneapolis 55455
    J Biol Chem 263:11692-6. 1988
    ..Evidence is also presented for the existence of an ATP-dependent amidating enzyme which catalyzes the final step in the biosynthesis of diphthamide in EF-2...
  70. pmc ADP ribosylation of human neutrophil peptide-1 regulates its biological properties
    Gregorino Paone
    Pulmonary Critical Care Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, 10 Center Drive, Bldg 10 Rm 6D03, Bethesda, MD 20892 1590, USA
    Proc Natl Acad Sci U S A 99:8231-5. 2002
    ..Thus, airway mono-ADP-ribosyltransferases could have an important regulatory role in the innate immune response through modification of alpha defensin-1 and perhaps other basic molecules, with alteration of their biological properties...
  71. pmc AtNUDT7, a negative regulator of basal immunity in Arabidopsis, modulates two distinct defense response pathways and is involved in maintaining redox homeostasis
    Xiaochun Ge
    Donald Danforth Plant Science Center, St Louis, MO 63132, USA
    Plant Physiol 145:204-15. 2007
    ..The study suggests that the alteration in cellular antioxidant status caused by the nudt7 mutation primes the cells for the amplified defense response and NUDT7 functions to modulate the defense response to prevent excessive stimulation...
  72. ncbi Analysis of Arabidopsis growth factor gene 1 (GFG1) encoding a nudix hydrolase during oxidative signaling
    Niranjani Jambunathan
    Department of Biochemistry and Molecular Biology, 246 Noble Research Center, Oklahoma State University, Stillwater, OK 74078, USA
    Planta 224:1-11. 2006
    ..Based on rapid transcriptional changes in response to various stresses, substrate specificity of the enzyme, and analysis of the knock out mutant, we propose that GFG1 is a key gene linking cellular metabolism and oxidative signaling...
  73. ncbi Sustained depolarization and ADP-ribose activate a common ionic current in rat peritoneal macrophages
    Brice Campo
    Institute of Molecular Physiology, University of Sheffield, Sheffield, United Kingdom
    J Immunol 170:1167-73. 2003
    ..The results suggest that prolonged depolarization leads to an increased intracellular level of ADP-ribose, which in turn activates this nonselective conductance(s)...
  74. ncbi Sir2 regulation by nicotinamide results from switching between base exchange and deacetylation chemistry
    Anthony A Sauve
    Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Biochemistry 42:9249-56. 2003
    ..Sir2 intermediate. These findings suggest a strategy for increasing Sir2 enzyme catalytic activity in vivo by inhibition of chemical exchange but not deacetylation...
  75. ncbi How bacterial ADP-ribosylating toxins recognize substrates
    Jianjun Sun
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, Wisconsin 53226, USA
    Nat Struct Mol Biol 11:868-76. 2004
    ..The electrostatic properties of regions A, B, C and E define the substrate specificity of ExoS and ExoT and these interactions can explain how other bacterial ADP-ribosylating toxins recognize their unique substrates...
  76. ncbi Structural basis for the mechanism and regulation of Sir2 enzymes
    Jose L Avalos
    Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205 USA
    Mol Cell 13:639-48. 2004
    ..We propose a detailed structure-based mechanism for deacetylation and nicotinamide inhibition of Sir2 consistent with mutagenesis and enzymatic studies...
  77. ncbi Processing of ADP-ribosylated integrin alpha 7 in skeletal muscle myotubes
    A Zolkiewska
    Pulmonary Critical Care Medicine Branch, NHLBI, National Institutes of Health, Bethesda, Maryland 20892 1434, USA
    J Biol Chem 270:9227-33. 1995
    ..Thus, cell surface ADP-ribosylation, in contrast to intracellular ADP-ribosylation, is not readily reversed by ADP-ribosylarginine hydrolase and seems to operate outside the postulated ADP-ribosylation cycle...
  78. ncbi Immunocyte Ca2+ influx system mediated by LTRPC2
    Y Sano
    Molecular Medicine Laboratories, Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co, Ltd, 21 Miyukigaoka, Tsukuba, Ibaraki 305 8585, Japan
    Science 293:1327-30. 2001
    ..This activation was suppressed by intracellular adenosine triphosphate. These results reveal that ADPR and NAD act as intracellular messengers and may have an important role in Ca2+ influx by activating LTRPC2 in immunocytes...
  79. ncbi Structural identification of 2'- and 3'-O-acetyl-ADP-ribose as novel metabolites derived from the Sir2 family of beta -NAD+-dependent histone/protein deacetylases
    Michael D Jackson
    Oregon Health and Science University, Department of Biochemistry and Molecular Biology, Portland, Oregopn 97201 3098, USA
    J Biol Chem 277:18535-44. 2002
    ..Possible mechanisms for the generation of 2'-O-acetyl-ADP-ribose are discussed...
  80. ncbi Human placenta hydrolases active on free ADP-ribose: an ADP-sugar pyrophosphatase and a specific ADP-ribose pyrophosphatase
    J M Ribeiro
    , Facultad de Medicina, Universidad de Extremadura, Apartado de Correos 108, E-06080 Badajoz, Spain
    Biochim Biophys Acta 1526:86-94. 2001
    ..It remains to be seen whether it belongs to the 'nudix' protein family, which includes several ADP-ribose hydrolases and other 'housecleaning' enzymes (M.J. Bessman, D.N. Frick, S.F. O'Handley, J. Biol. Chem. 271 (1996) 25059-25062)...
  81. pmc The Poly(ADP-ribose) polymerase PARP-1 is required for oxidative stress-induced TRPM2 activation in lymphocytes
    Ben Buelow
    Department of Pediatrics and Immunology, University of Washington, Seattle, Washington 98103, USA
    J Biol Chem 283:24571-83. 2008
    ..Together, our results suggest that during conditions of oxidative stress in lymphocytes, TRPM2 acts as a downstream effector of the PARP/poly(ADP-ribose) glycohydrolase pathway through PARP-dependent formation of ADP-ribose...
  82. pmc Characterization of the Saccharomyces cerevisiae cyclic nucleotide phosphodiesterase involved in the metabolism of ADP-ribose 1",2"-cyclic phosphate
    F Nasr
    Friedrich Miescher Institut, PO Box 2543, CH 4002 Basel, Switzerland
    Nucleic Acids Res 28:1676-83. 2000
    ..Likewise, overexpression of Cpd1p had no effect on yeast growth. These results do not implicate an important role for Appr>p or Appr-1"p in yeast cells grown under standard laboratory conditions...
  83. pmc Crystal structures of two coronavirus ADP-ribose-1''-monophosphatases and their complexes with ADP-Ribose: a systematic structural analysis of the viral ADRP domain
    Yuanyuan Xu
    Laboratory of Structural Biology, Tsinghua University, Beijing, China
    J Virol 83:1083-92. 2009
    ....
  84. doi Identification of lysines 36 and 37 of PARP-2 as targets for acetylation and auto-ADP-ribosylation
    Sandra S Haenni
    Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    Int J Biochem Cell Biol 40:2274-83. 2008
    ..Together, our results provide evidence that acetylation of PARP-2 is a key post-translational modification that may regulate DNA binding and consequently also the enzymatic activity of PARP-2...
  85. ncbi CD38 disruption impairs glucose-induced increases in cyclic ADP-ribose, [Ca2+]i, and insulin secretion
    I Kato
    Department of Biochemistry, Tohoku University School of Medicine, Sendai 980 8575, Miyagi, Japan
    J Biol Chem 274:1869-72. 1999
    ..These results indicate that CD38 plays an essential role in intracellular Ca2+ mobilization by cADPR for insulin secretion...
  86. doi Overexpression of an ADP-ribose pyrophosphatase, AtNUDX2, confers enhanced tolerance to oxidative stress in Arabidopsis plants
    Takahisa Ogawa
    Department of Advanced Bioscience, Faculty of Agriculture, Kinki University, 3327 204 Nakamachi, Nara 631 8505, Japan
    Plant J 57:289-301. 2009
    ....
  87. doi A calcium influx pathway regulated separately by oxidative stress and ADP-Ribose in TRPM2 channels: single channel events
    Mustafa Naziroglu
    Department of Biophysics, Medical Faculty, Suleyman Demirel University, Cunur, Isparta 32260, Turkey
    Neurochem Res 33:1256-62. 2008
    ..A second novel result of the present study was that the TRPM2 channels were constitutionally activated by H2O2...
  88. pmc Intracellular calcium activates TRPM2 and its alternative spliced isoforms
    Jianyang Du
    Department of Cell Biology, Calhoun Cardiology Center, University of Connecticut Health Center, Farmington, CT 06030, USA
    Proc Natl Acad Sci U S A 106:7239-44. 2009
    ....
  89. pmc Cloning, expression and characterization of YSA1H, a human adenosine 5'-diphosphosugar pyrophosphatase possessing a MutT motif
    L Gasmi
    School of Biological Sciences, Life Sciences Building, University of Liverpool, Liverpool L69 7ZB, U K
    Biochem J 344:331-7. 1999
    ..Hence the function of YSA1H might be to remove free ADP-ribose arising from NAD(+) and protein-bound poly- and mono-(ADP-ribose) turnover to prevent the occurrence of non-enzymic protein glycation...
  90. pmc Crystal structures of the X-domains of a Group-1 and a Group-3 coronavirus reveal that ADP-ribose-binding may not be a conserved property
    Yvonne Piotrowski
    Institute of Biochemistry, Center for Structural and Cell Biology in Medicine, University of Lubeck, Ratzeburger Allee 160, 23538 Lubeck, Germany
    Protein Sci 18:6-16. 2009
    ..For comparison, we also describe the crystal structure of the homologous X-domain from Human Coronavirus 229E, a Group-1 coronavirus, which does bind ADP-ribose...
  91. ncbi Actin is ADP-ribosylated by the Salmonella enterica virulence-associated protein SpvB
    D Tezcan-Merdol
    Microbiology and Tumor Biology Center, Karolinska Institute, , 171 77 Stockholm, Sweden
    Mol Microbiol 39:606-19. 2001
    ..We conclude that actin is a target for an SpvB-mediated modification, most probably ADP-ribosylation, and that the modification of G actin interferes with actin polymerization...
  92. pmc Differential regulation of nicotinic acid-adenine dinucleotide phosphate and cADP-ribose production by cAMP and cGMP
    H L Wilson
    University Department of Pharmacology, Oxford University, Mansfield Road, Oxford OX1 3QT, UK
    Biochem J 331:837-43. 1998
    ..We suggest that in the sea urchin egg, cADPR and NAADP production may be differentially regulated by receptor-coupled second messengers, with cADPR production being regulated by cGMP and NAADP production modulated by cAMP...
  93. ncbi Regulatory role of CD38 (ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase) in insulin secretion by glucose in pancreatic beta cells. Enhanced insulin secretion in CD38-expressing transgenic mice
    I Kato
    Department of Biochemistry, Tohoku University School of Medicine, Miyagi, Japan
    J Biol Chem 270:30045-50. 1995
    ....
  94. pmc Structural basis for nicotinamide cleavage and ADP-ribose transfer by NAD(+)-dependent Sir2 histone/protein deacetylases
    Kehao Zhao
    The Wistar Institute, Department of Biochemistry and Biophysics, School of Medicine, and Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104
    Proc Natl Acad Sci U S A 101:8563-8. 2004
    ..Together, these studies provide insights into the chemistry of NAD(+) cleavage and acetylation by Sir2 proteins and have implications for the design of Sir2-specific regulatory molecules...
  95. ncbi Actin--an inhibitor of eukaryotic elongation factor activities
    Muhammet Bektaş
    Biophysics Department, Istanbul Faculty of Medicine, Istanbul University, 34390, Capa, Istanbul, Turkey
    Biochem Biophys Res Commun 317:1061-6. 2004
    ..Some component(s) in the postribosomal supernatant (S-130) fraction and also DNase I prevent actin-promoted inhibition of polyPhe synthesis...
  96. pmc The silencing protein SIR2 and its homologs are NAD-dependent protein deacetylases
    J Landry
    Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794 5215, USA
    Proc Natl Acad Sci U S A 97:5807-11. 2000
    ..Discovery of an intrinsic deacetylation activity for the conserved SIR2 family provides a mechanism for modifying histones and other proteins to regulate transcription and diverse biological processes...
  97. ncbi Extracellular cyclic ADP-ribose potentiates ACh-induced contraction in bovine tracheal smooth muscle
    L Franco
    Biocrystallography Centre-Consiglio Nazionale delle Ricerche, University Federico II, 80134 Naples, Italy
    Am J Physiol Lung Cell Mol Physiol 280:L98-L106. 2001
    ..These data suggest the existence of a paracrine mechanism whereby mucosa-released extracellular NAD(+) plays a hormonelike function and cADPR behaves as second messenger regulating calcium-related contractility in TSMs...
  98. ncbi Transition state structure for ADP-ribosylation of eukaryotic elongation factor 2 catalyzed by diphtheria toxin
    Sapan L Parikh
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Biochemistry 43:1204-12. 2004
    ..The transition state model presented here is asymmetric and consistent with a dissociative S(N)1 type mechanism in which attack of the diphthamide nucleophile lags behind departure of the nicotinamide...
  99. ncbi Toward the elucidation of the catalytic mechanism of the mono-ADP-ribosyltransferase activity of Pseudomonas aeruginosa exotoxin A
    Souzan Armstrong
    Guelph Waterloo Centre for Graduate Work in Chemistry and Biochemistry, Department of Chemistry and Biochemistry, University of Guelph, Ontario N1G 2W1 Canada
    Biochemistry 43:183-94. 2004
    ..Detailed kinetic analysis revealed that the eEF-2 substrate shows sigmoidal kinetic behavior with the enzyme, and fluorescence resonance energy transfer measurements indicated that wheat germ eEF-2 is oligomeric in solution...
  100. ncbi Endogenous ADP-ribosylation for eukaryotic elongation factor 2: evidence of two different sites and reactions
    Muhammet Bektaş
    Istanbul University, Istanbul Faculty of Medicine, Department of Biophysics, 34390 Capa, Istanbul, Turkey
    Cell Biochem Funct 24:369-80. 2006
    ..The respective sites involved in these reactions are distinct from one another as well as from diphthamide, the site of attack by diphtheria toxin...
  101. ncbi A new function for CD38/ADP-ribosyl cyclase in nuclear Ca2+ homeostasis
    O A Adebanjo
    Department of Medicine, Medical College of Pennsylvania School of Medicine and Veterans Affairs Medical Center, Philadelphia, Pennsylvania 19104, USA
    Nat Cell Biol 1:409-14. 1999
    ..that the enzyme catalyses the intranuclear cyclization of nicotinamide adenine dinucleotide to cyclic adenosine diphosphate ribose. The latter activates ryanodine receptors of the inner nuclear membrane to trigger nucleoplasmic Ca2+ ..

Research Grants84

  1. Chymase-Mediated MMP Activation in Ishemia Reperfusion Injury
    LOUIS DELL ITALIA; Fiscal Year: 2009
    ....
  2. Combination drug treatment to inhibit multiple cell death pathways after TBI
    ALAN FADEN; Fiscal Year: 2009
    ....
  3. Combination drug treatment to inhibit multiple cell death pathways after TBI
    ALAN IRA FADEN; Fiscal Year: 2010
    ....
  4. Combination drug treatment to inhibit multiple cell death pathways after TBI
    ALAN FADEN; Fiscal Year: 2009
    ....
  5. Targeting EWS/FLI1-driven pathways to improve therapeutic gains in Ewing's Sarcom
    Vicente Notario; Fiscal Year: 2009
    ..Treatments with inhibitors of PARP-1, a molecule previously shown in our Department to contribute to the radiation resistance of ESFT, will be included also in our experimental protocols. ..
  6. Targeting EWS/FLI1-driven pathways to improve therapeutic gains in Ewing's Sarcom
    Vicente Notario; Fiscal Year: 2010
    ..Treatments with inhibitors of PARP-1, a molecule previously shown in our Department to contribute to the radiation resistance of ESFT, will be included also in our experimental protocols. ..
  7. Local Calcium Signaling in Pulmonary Arteries
    JAMES SHAM; Fiscal Year: 2009
    ..The information generated by the proposed experiments will be valuable for the development of new treatments for pulmonary vascular diseases. ..
  8. Local Calcium Signaling in Pulmonary Arteries
    JAMES SK SHAM; Fiscal Year: 2010
    ..The information generated by the proposed experiments will be valuable for the development of new treatments for pulmonary vascular diseases. ..
  9. BBB Protection in HIV Infection: Barrier-shielding effects of PARP inhibition
    Yuri Persidsky; Fiscal Year: 2012
    ..PARP inhibitors have now reached the stage of clinical testing for cancer treatment, assuring quick translation to therapy of immune/inflammatory disorders. ..
  10. Arsenic-enhanced skin carcinogenesis by UV radiation
    Ke Liu; Fiscal Year: 2009
    ....
  11. Mechanisms and Modulation of Cell Death in Traumatic Brain Injury
    ALAN IRA FADEN; Fiscal Year: 2010
    ..A better understanding of the mechanisms underlying TBI would offer the possibility of improving survival and insuring a more complete recovery. ..
  12. Mechanisms and Modulation of Cell Death in Traumatic Brain Injury
    ALAN IRA FADEN; Fiscal Year: 2009
    ..A better understanding of the mechanisms underlying TBI would offer the possibility of improving survival and insuring a more complete recovery. ..
  13. Mechanisms and Modulation of Cell Death in Traumatic Brain Injury
    ALAN FADEN; Fiscal Year: 2009
    ..A better understanding of the mechanisms underlying TBI would offer the possibility of improving survival and insuring a more complete recovery. ..
  14. PARP inhibitor therapy for septic shock
    Andrew Salzman; Fiscal Year: 2004
    ..Based on pre-clinical porcine shock models and clinical safety studies, we expect INO-1001 to be a safe, well-tolerated and effective therapeutic in patients with septic shock. ..
  15. CALCIUM REGULATION SYSTEMS IN SEA URCHIN EGGS
    HONCHEUNG LEE; Fiscal Year: 1990
    ..These studies will be extended to other cell systems to determine if cADPR is a general second messenger for intracellular Ca+2 mobilization similar to IP3...
  16. NAD & CD38 Form a Communication System in the Retina
    Robert Miller; Fiscal Year: 2005
    ..If this hypothesis can be experimentally established, it will form the basis of an entirely new method by which neurons and glia support each other to stabilize retinal function. ..
  17. NAD & CD38 Form a Communication System in the Retina
    Robert Miller; Fiscal Year: 2002
    ..If this hypothesis can be experimentally established, it will form the basis of an entirely new method by which neurons and glia support each other to stabilize retinal function. ..
  18. NAD & CD38 Form a Communication System in the Retina
    Robert Miller; Fiscal Year: 2004
    ..If this hypothesis can be experimentally established, it will form the basis of an entirely new method by which neurons and glia support each other to stabilize retinal function. ..
  19. NAD & CD38 Form a Communication System in the Retina
    Robert Miller; Fiscal Year: 2006
    ..If this hypothesis can be experimentally established, it will form the basis of an entirely new method by which neurons and glia support each other to stabilize retinal function. ..
  20. NAD & CD38 Form a Communication System in the Retina
    Robert Miller; Fiscal Year: 2003
    ..If this hypothesis can be experimentally established, it will form the basis of an entirely new method by which neurons and glia support each other to stabilize retinal function. ..
  21. A Redox-active PARP Inhibitor for Lung Transplantation
    ANDREW LURIE SALZMAN; Fiscal Year: 2012
    ..At present there are no approved prophylactic measures. We are developing a novel drug that targets the basic mechanisms of this condition and will test this agent in two clinically-relevant animal models. ..
  22. The role of two-pore channels in integrative calcium signaling
    Michael X Zhu; Fiscal Year: 2010
    ....
  23. Chemical approaches for studying the biology of CD38
    Hening Lin; Fiscal Year: 2011
    ..The enzymatic activity can convert nicotinamide adenine dinucleotide (NAD) to adenosine diphosphate ribose (ADPR) and cyclic ADPR (cADPR), and NAD phosphate (NADP) to nicotinic acid adenine dinucleotide ..
  24. Significance of Caspase-Mediated Cleavage of PARP-1 in Cell Death
    KAREN DAVID; Fiscal Year: 2007
    ..We believe that understanding the functional significance of this cleavage will help in developing therapeutic intervention to combat aging-related diseases and cell death. [unreadable] [unreadable] [unreadable]..
  25. SIGNAL TRANSDUCTION IN T CELLS
    CONSTANTINE TSOUKAS; Fiscal Year: 1990
    ..We'll measure the effects of these agents on IP3 and Ca++ accumulation using a permeabilized cell system and electroporation...
  26. MECHANISM OF CORONARY ENDOTHELIUM MEDIATED VASODILATION
    Pin Lan Li; Fiscal Year: 1999
    ..These studies will demonstrate a new signaling pathway for the action of endothelium-derived vasodilators and contribute to our understanding of cellular and molecular mechanism gating K+ channels and regulating coronary vascular tone. ..
  27. MECHANISM OF CORONARY ENDOTHELIUM MEDIATED VASODILATION
    Pin Lan Li; Fiscal Year: 2000
    ..These studies will demonstrate a new signaling pathway for the action of endothelium-derived vasodilators and contribute to our understanding of cellular and molecular mechanism gating K+ channels and regulating coronary vascular tone. ..
  28. Therapeutic Potential of PARP inhibitor for Acute Pancreatitis
    Latha Paka; Fiscal Year: 2010
    ..Our therapeutic strategy in the current project is to test our lead PARP inhibitors in two invivo models of AP and identifify one of the best PARP inhibitor to further studies in Phase II and potentially a clinical product. ..
  29. CYCLIC ADP RIBOSE METABOLISM IN OXIDATIVE CELL DEATH
    MYRON JACOBSON; Fiscal Year: 1999
    ....
  30. CYCLIC ADP RIBOSE METABOLISM IN OXIDATIVE CELL DEATH
    MYRON JACOBSON; Fiscal Year: 2002
    ....
  31. CYCLIC ADP RIBOSE METABOLISM IN OXIDATIVE CELL DEATH
    MYRON JACOBSON; Fiscal Year: 2001
    ....
  32. CYCLIC ADP RIBOSE METABOLISM IN OXIDATIVE CELL DEATH
    MYRON JACOBSON; Fiscal Year: 2000
    ....
  33. CYCLIC ADP RIBOSE METABOLISM IN OXIDATIVE CELL DEATH
    MYRON JACOBSON; Fiscal Year: 2000
    ....
  34. PARS inhibitor therapy of smoke inhalation injury
    Prakash Jagtap; Fiscal Year: 2001
    ..Estimated worldwide gross sales revenues after market entry and maturation (ca. 4 years after FDA approval) equal $250 million (annual). ..
  35. PARS inhibitor therapy for smoke inhalation
    Andrew Salzman; Fiscal Year: 2004
    ..The proposed clinical trial is expected to provide the foundation for FDA orphan drug approval of INO-1001 for treatment of acute smoke inhalation injury. ..
  36. PARS inhibitor therapy for smoke inhalation
    Andrew Salzman; Fiscal Year: 2003
    ..The proposed clinical trial is expected to provide the foundation for FDA orphan drug approval of INO-1001 for treatment of acute smoke inhalation injury. ..
  37. Role of poly (ADP-ribose)in the Repair of Ultraviolet Induced DNA Damage
    BRENEE SHEMEIR KING; Fiscal Year: 2011
    ..UVR is the major environmental agent contributing to skin cancer and this project is aimed at gaining a molecular understanding of PARP's role in DNA repair activities. ..
  38. Regulation of IL4 signal transduciton by poly (ADP-ribose) polymerase-1.
    Rahul Datta; Fiscal Year: 2009
    ..Given the data demonstrating that PARP-1 inhibition abates of pulmonary inflammation in the mouse model of asthma, it is important to study the role of PARP-1 in inflammation in order to develop strategies in dealing with the disease. ..
  39. ADP RIBOSYLATION OF A POTENT INDUCER OF PROLIFERATION
    Mark Francis; Fiscal Year: 2002
    ..Using NAD as a source, bacterial toxins add adenosine diphosphate ribose (ADP-ribose) to proteins, a process called ADP-ribosylation...
  40. The Role of PARP-SIR2 Signaling in Heart Failure
    Mahesh Gupta; Fiscal Year: 2007
    ..As such, these studies are likely to provide new strategies for the management of heart failure. ..
  41. ADP RIBOSYLATION OF A POTENT INDUCER OF PROLIFERATION
    Mark Francis; Fiscal Year: 1999
    ..Using NAD as a source, bacterial toxins add adenosine diphosphate ribose (ADP-ribose) to proteins, a process called ADP-ribosylation...
  42. Regulation of IL4 signal transduciton by poly (ADP-ribose) polymerase-1.
    Rahul Datta; Fiscal Year: 2010
    ..Given the data demonstrating that PARP-1 inhibition abates of pulmonary inflammation in the mouse model of asthma, it is important to study the role of PARP-1 in inflammation in order to develop strategies in dealing with the disease. ..
  43. The Role of PARP-SIR2 Signaling in Heart Failure
    Mahesh Gupta; Fiscal Year: 2009
    ..As such, these studies are likely to provide new strategies for the management of heart failure. ..
  44. ADP RIBOSYLATION OF A POTENT INDUCER OF PROLIFERATION
    Mark Francis; Fiscal Year: 2000
    ..Using NAD as a source, bacterial toxins add adenosine diphosphate ribose (ADP-ribose) to proteins, a process called ADP-ribosylation...
  45. ADP RIBOSYLATION OF A POTENT INDUCER OF PROLIFERATION
    Mark Francis; Fiscal Year: 2001
    ..Using NAD as a source, bacterial toxins add adenosine diphosphate ribose (ADP-ribose) to proteins, a process called ADP-ribosylation...
  46. The Role of PARP-SIR2 Signaling in Heart Failure
    Mahesh P Gupta; Fiscal Year: 2010
    ..As such, these studies are likely to provide new strategies for the management of heart failure. ..
  47. Nitric Oxide--Neurotoxic Mechanisms of MPTP
    Allen Mandir; Fiscal Year: 2001
    ..In this study we demonstrate that NMDA excitotoxicity is dramatically reduced in PARP-1 knockout mice whereas PARP-1 knockout mice are sensitive to AMPA excitotoxicity. ..
  48. Nitric Oxide--Neurotoxic Mechanisms of MPTP
    Allen Mandir; Fiscal Year: 2002
    ..In this study we demonstrate that NMDA excitotoxicity is dramatically reduced in PARP-1 knockout mice whereas PARP-1 knockout mice are sensitive to AMPA excitotoxicity. ..
  49. Volatile Anesthesic Inhibition of Ca2+ Release in Airway
    Gary Sieck; Fiscal Year: 2006
    ..The studies will initially focus on the effects of halothane; however, when significant effects are observed, the effects of isoflurane and sevoflurane will also be explored. ..
  50. Volatile Anesthesic Inhibition of Ca2+ Release in Airway
    Gary Sieck; Fiscal Year: 2003
    ..The studies will initially focus on the effects of halothane; however, when significant effects are observed, the effects of isoflurane and sevoflurane will also be explored. ..
  51. BONE GROWTH IN DENTAL, CRANIAL AND SKELETAL TISSUES
    Irving Shapiro; Fiscal Year: 1999
    ..The analysis will consist of FACTS flow cytometry based on alkaline phosphatase or annexin content and phospholipid packing. ..
  52. BONE GROWTH IN DENTAL, CRANIAL AND SKELETAL TISSUES
    Irving Shapiro; Fiscal Year: 2001
    ..The analysis will consist of FACTS flow cytometry based on alkaline phosphatase or annexin content and phospholipid packing. ..
  53. BONE GROWTH IN DENTAL, CRANIAL AND SKELETAL TISSUES
    Irving Shapiro; Fiscal Year: 2000
    ..The analysis will consist of FACTS flow cytometry based on alkaline phosphatase or annexin content and phospholipid packing. ..
  54. REVERSIBLE ADP-RIBOSYLATION OF NITROGENASE
    PAUL LUDDEN; Fiscal Year: 1999
    ..The Mn-binding site of DRAG will be characterized and the role of Mn in stimulating/inhibiting any complex of DRAG with other proteins will be investigated. The hypothesis that Mn constitutes a binuclear cluster in DRAG will be tested. ..
  55. Poly(ADP-ribose) polymerase in diabetic nephropathy
    IRINA OBROSOVA; Fiscal Year: 2005
    ..abstract_text> ..
  56. Calcium in the regulation of osteoclast formation
    Mone Zaidi; Fiscal Year: 2003
    ..Finally, we will determine whether CD38 over-expression in transgenic mice results in an osteopetrotic phenotype and dysfunctional osteoclasts...
  57. Calcium in the regulation of osteoclast formation
    Mone Zaidi; Fiscal Year: 2007
    ..Finally, we will determine whether CD38 over-expression in transgenic mice results in an osteopetrotic phenotype and dysfunctional osteoclasts...
  58. Calcium in the regulation of osteoclast formation
    Mone Zaidi; Fiscal Year: 2004
    ..Finally, we will determine whether CD38 over-expression in transgenic mice results in an osteopetrotic phenotype and dysfunctional osteoclasts...
  59. Calcium in the regulation of osteoclast formation
    Mone Zaidi; Fiscal Year: 2006
    ..Finally, we will determine whether CD38 over-expression in transgenic mice results in an osteopetrotic phenotype and dysfunctional osteoclasts...
  60. Calcium in the regulation of osteoclast formation
    Mone Zaidi; Fiscal Year: 2005
    ..Finally, we will determine whether CD38 over-expression in transgenic mice results in an osteopetrotic phenotype and dysfunctional osteoclasts...
  61. Poly(ADP-ribose) polymerase in diabetic nephropathy
    IRINA OBROSOVA; Fiscal Year: 2006
    ..abstract_text> ..
  62. Role of poly(ADP-ribosyl)ation in diabetic neuropathy
    IRINA OBROSOVA; Fiscal Year: 2003
    ..The studies are of direct relevance to the pathogenesis of PDN and may provide the rationale for development of PARS inhibitors for its prevention and treatment. ..
  63. Role of poly(ADP-ribosyl)ation in diabetic neuropathy
    IRINA OBROSOVA; Fiscal Year: 2002
    ..The studies are of direct relevance to the pathogenesis of PDN and may provide the rationale for development of PARS inhibitors for its prevention and treatment. ..
  64. Role of poly(ADP-ribosyl)ation in diabetic neuropathy
    IRINA OBROSOVA; Fiscal Year: 2004
    ..The studies are of direct relevance to the pathogenesis of PDN and may provide the rationale for development of PARS inhibitors for its prevention and treatment. ..
  65. REGULATION OF MACROPHAGE DEPENDENT ANGIOGENIC ACTIVITY
    SAMUEL LEIBOVICH; Fiscal Year: 1999
    ..abstract_text> ..
  66. REGULATION OF MACROPHAGE DEPENDENT ANGIOGENIC ACTIVITY
    SAMUEL LEIBOVICH; Fiscal Year: 2000
    ..abstract_text> ..
  67. REGULATION OF MACROPHAGE DEPENDENT ANGIOGENIC ACTIVITY
    SAMUEL LEIBOVICH; Fiscal Year: 2002
    ..abstract_text> ..
  68. REGULATION OF MACROPHAGE DEPENDENT ANGIOGENIC ACTIVITY
    SAMUEL LEIBOVICH; Fiscal Year: 2001
    ..abstract_text> ..
  69. Mechanism of Coronary Endothelium-mediated Vasodilation
    Pin Lan Li; Fiscal Year: 2002
    ..These studies will define a new signaling mechanism regulating [Ca2+]i and vasomotor response in coronary resistance arteries and increase our understanding of the cellular mechanism mediating the vasodilator effect of NO. ..
  70. Mechanism of Coronary Endothelium-mediated Vasodilation
    Pin Lan Li; Fiscal Year: 2003
    ..These studies will define a new signaling mechanism regulating [Ca2+]i and vasomotor response in coronary resistance arteries and increase our understanding of the cellular mechanism mediating the vasodilator effect of NO. ..
  71. Mechanism of Coronary Endothelium-mediated Vasodilation
    Pin Lan Li; Fiscal Year: 2004
    ..These studies will define a new signaling mechanism regulating [Ca2+]i and vasomotor response in coronary resistance arteries and increase our understanding of the cellular mechanism mediating the vasodilator effect of NO. ..
  72. Mechanism of Coronary Endothelium-mediated Vasodilation
    Pin Lan Li; Fiscal Year: 2001
    ..These studies will define a new signaling mechanism regulating [Ca2+]i and vasomotor response in coronary resistance arteries and increase our understanding of the cellular mechanism mediating the vasodilator effect of NO. ..
  73. Mechanism of Coronary Endothelium-mediated Vasodilation
    Pin Lan Li; Fiscal Year: 2004
    ..These studies will define a new signaling mechanism regulating [Ca2+]i and vasomotor response in coronary resistance arteries and increase our understanding of the cellular mechanism mediating the vasodilator effect of NO. ..
  74. Mayo Clinic SPORE in Ovarian Cancer
    Lynn C Hartmann; Fiscal Year: 2010
    ..The Mayo Clinic SPORE in Ovarian Cancer has the full support of the Mayo Clinic Cancer Center and Mayo Foundation. ..
  75. MECHANISMS OF CELL REPLICATION IN ASBESTOS CANCERS
    Brooke Mossman; Fiscal Year: 1993
    ..Mossman, a cell biologist with expertise in asbestos and AOS, Dr. Heintz, a molecular biologist with expertise in cell replication, and Dr. Leslie, a lung pathologist with expertise in immunocytochemistry...
  76. REVERSIBLE ADP-RIBOSYLATION OF NITROGENASE
    PAUL LUDDEN; Fiscal Year: 2003
    ..ADP-ribosylation is increasingly recognized as an important mechanism of regulation in eucaryotes and procaryotes. A number of pathogenic bacteria produce toxins that ADP-ribosylate crucial protein targets in their eucaryotic hosts. ..
  77. REVERSIBLE ADP-RIBOSYLATION OF NITROGENASE
    PAUL LUDDEN; Fiscal Year: 2002
    ..ADP-ribosylation is increasingly recognized as an important mechanism of regulation in eucaryotes and procaryotes. A number of pathogenic bacteria produce toxins that ADP-ribosylate crucial protein targets in their eucaryotic hosts. ..
  78. REVERSIBLE ADP-RIBOSYLATION OF NITROGENASE
    PAUL LUDDEN; Fiscal Year: 2002
    ..ADP-ribosylation is increasingly recognized as an important mechanism of regulation in eucaryotes and procaryotes. A number of pathogenic bacteria produce toxins that ADP-ribosylate crucial protein targets in their eucaryotic hosts. ..
  79. REVERSIBLE ADP-RIBOSYLATION OF NITROGENASE
    PAUL LUDDEN; Fiscal Year: 2001
    ..ADP-ribosylation is increasingly recognized as an important mechanism of regulation in eucaryotes and procaryotes. A number of pathogenic bacteria produce toxins that ADP-ribosylate crucial protein targets in their eucaryotic hosts. ..
  80. Mayo Clinic SPORE in Ovarian Cancer
    Lynn Hartmann; Fiscal Year: 2009
    ..The Mayo Clinic SPORE in Ovarian Cancer has the full support of the Mayo Clinic Cancer Center and Mayo Foundation. ..
  81. REVERSIBLE ADP-RIBOSYLATION OF NITROGENASE
    PAUL LUDDEN; Fiscal Year: 2000
    ..ADP-ribosylation is increasingly recognized as an important mechanism of regulation in eucaryotes and procaryotes. A number of pathogenic bacteria produce toxins that ADP-ribosylate crucial protein targets in their eucaryotic hosts. ..
  82. DYNAMIC CALCIUM REGULATION IN AIRWAY SMOOTH MUSCLE
    Mathur S Kannan; Fiscal Year: 2010
    ..This new information may identify CD38 as a potential pharmacological target in the prevention and control of asthma. ..