thymidine

Summary

Top Publications

  1. doi Kinetics of hepatitis B surface antigen decline during 3 years of telbivudine treatment in hepatitis B e antigen-positive patients
    Karsten Wursthorn
    Department of Gastroenterology, Hepatology, and Endocrinology, Hannover Medical School, Hannover, Germany
    Hepatology 52:1611-20. 2010
  2. pmc Significant human beta-cell turnover is limited to the first three decades of life as determined by in vivo thymidine analog incorporation and radiocarbon dating
    S Perl
    Diabetes Branch, National Institute of Diabetes, Digestive, and Kidney Disease NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Clin Endocrinol Metab 95:E234-9. 2010
  3. doi EdU, a new thymidine analogue for labelling proliferating cells in the nervous system
    Fatemah Chehrehasa
    National Centre for Adult Stem Cell Research, Eskitis Institute for Cellular and Molecular Therapies, Griffith University, Brisbane, QLD 4111, Australia
    J Neurosci Methods 177:122-30. 2009
  4. doi Efficacy and safety of prolonged 3-year telbivudine treatment in patients with chronic hepatitis B
    Edward J Gane
    New Zealand Liver Unit, Auckland City Hospital, Auckland, New Zealand
    Liver Int 31:676-84. 2011
  5. doi Myopathy and neuropathy associated with nucleos(t)ide analog therapy for hepatitis B
    Russell D Fleischer
    Division of Antiviral Products, Center for Drug Evaluation and Research, Food and Drug Administration, Silver Spring, MD, USA
    J Hepatol 51:787-91. 2009
  6. pmc Thymidine-dependent small-colony variants of Staphylococcus aureus exhibit gross morphological and ultrastructural changes consistent with impaired cell separation
    Barbara C Kahl
    Institute of Medical Microbiology, University of Muenster Hospital and Clinics, Muenster, Germany
    J Clin Microbiol 41:410-3. 2003
  7. ncbi Stem cell proliferative history in tissue revealed by temporal halogenated thymidine analog discrimination
    Christopher J Vega
    Department of Neuroscience, The Chicago Medical School at Rosalind Franklin University of Medicine and Science, 3333 Green Bay Road, North Chicago, Illinois 60064, USA
    Nat Methods 2:167-9. 2005
  8. ncbi Molecular determinants of multi-nucleoside analogue resistance in HIV-1 reverse transcriptases containing a dipeptide insertion in the fingers subdomain: effect of mutations D67N and T215Y on removal of thymidine nucleotide analogues from blocked DNA prim
    Tania Matamoros
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas Universidad Autonoma de Madrid, 28049 Madrid, Spain
    J Biol Chem 279:24569-77. 2004
  9. pmc Quantification of oxidative DNA lesions in tissues of Long-Evans Cinnamon rats by capillary high-performance liquid chromatography-tandem mass spectrometry coupled with stable isotope-dilution method
    Jin Wang
    Department of Chemistry, University of California, Riverside, California 92521 0403, United States
    Anal Chem 83:2201-9. 2011
  10. ncbi Telbivudine versus lamivudine in patients with chronic hepatitis B
    Ching Lung Lai
    University Department of Medicine, Queen Mary Hospital, Hong Kong, China
    N Engl J Med 357:2576-88. 2007

Research Grants

  1. CSF-1 in Dental Biology
    SHERRY ABBOUD WERNER; Fiscal Year: 2007
  2. Enhancing Suicide Gene Therapy Through Mechanism-Based Approaches
    Donna S Shewach; Fiscal Year: 2010
  3. OCULAR HERPES SIMPLEX
    Herbert Kaufman; Fiscal Year: 1993
  4. Metabolism and toxicity of nucleoside reverse transcriptase inhibitors in non-rep
    Edward E McKee; Fiscal Year: 2010
  5. Clinical and Immunologic Evaluation of ProstAtak for Prostate Cancer
    Estuardo Aguilar Cordova; Fiscal Year: 2007
  6. Imaging Infused CD19 Specific T Cells in the Tumor
    LAURENCE COOPER; Fiscal Year: 2007
  7. CYTOTOXICITY AND BYSTANDER KILLING FOR HSV-TK SUBSTRATES
    Donna Shewach; Fiscal Year: 2000
  8. Mitochondrial Toxicity of Antiviral Nucleosides
    Edward E McKee; Fiscal Year: 2010
  9. DNA REPAIR IN A HORMONE RESPONSIVE GENE
    Michael Smerdon; Fiscal Year: 1999
  10. Boronated nucleosides for neutron capture therapy of brain tumors
    Werner Tjarks; Fiscal Year: 2010

Detail Information

Publications329 found, 100 shown here

  1. doi Kinetics of hepatitis B surface antigen decline during 3 years of telbivudine treatment in hepatitis B e antigen-positive patients
    Karsten Wursthorn
    Department of Gastroenterology, Hepatology, and Endocrinology, Hannover Medical School, Hannover, Germany
    Hepatology 52:1611-20. 2010
    ..In patients with subsequent HBsAg loss, viral antigens were already undetectable in liver biopsy samples after 1 year of treatment. This was associated with markedly enhanced antiviral T cell reactivity...
  2. pmc Significant human beta-cell turnover is limited to the first three decades of life as determined by in vivo thymidine analog incorporation and radiocarbon dating
    S Perl
    Diabetes Branch, National Institute of Diabetes, Digestive, and Kidney Disease NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Clin Endocrinol Metab 95:E234-9. 2010
    ..The turnover rate of adult human β-cells remains unknown. We employed two techniques to examine adult human islet β-cell turnover and longevity in vivo...
  3. doi EdU, a new thymidine analogue for labelling proliferating cells in the nervous system
    Fatemah Chehrehasa
    National Centre for Adult Stem Cell Research, Eskitis Institute for Cellular and Molecular Therapies, Griffith University, Brisbane, QLD 4111, Australia
    J Neurosci Methods 177:122-30. 2009
    ..We present here a novel thymidine analogue, ethynyl deoxyuridine (EdU) for labelling dividing cells, detected with a fluorescent azide which forms ..
  4. doi Efficacy and safety of prolonged 3-year telbivudine treatment in patients with chronic hepatitis B
    Edward J Gane
    New Zealand Liver Unit, Auckland City Hospital, Auckland, New Zealand
    Liver Int 31:676-84. 2011
    ..Aims: To investigate the long-term efficacy and safety of telbivudine in the telbivudine-treated cohort from the GLOBE trial...
  5. doi Myopathy and neuropathy associated with nucleos(t)ide analog therapy for hepatitis B
    Russell D Fleischer
    Division of Antiviral Products, Center for Drug Evaluation and Research, Food and Drug Administration, Silver Spring, MD, USA
    J Hepatol 51:787-91. 2009
    ....
  6. pmc Thymidine-dependent small-colony variants of Staphylococcus aureus exhibit gross morphological and ultrastructural changes consistent with impaired cell separation
    Barbara C Kahl
    Institute of Medical Microbiology, University of Muenster Hospital and Clinics, Muenster, Germany
    J Clin Microbiol 41:410-3. 2003
    b>Thymidine-dependent small-colony variants (SCV) of Staphylococcus aureus exhibited unusual colony morphology with "fried-egg" or pinpoint white colonies on agar plates and pleomorphic cocci as determined by Gram staining...
  7. ncbi Stem cell proliferative history in tissue revealed by temporal halogenated thymidine analog discrimination
    Christopher J Vega
    Department of Neuroscience, The Chicago Medical School at Rosalind Franklin University of Medicine and Science, 3333 Green Bay Road, North Chicago, Illinois 60064, USA
    Nat Methods 2:167-9. 2005
    ..We describe incorporation of different halogenated thymidine analogs for temporal discrimination of cell cycle in the rat...
  8. ncbi Molecular determinants of multi-nucleoside analogue resistance in HIV-1 reverse transcriptases containing a dipeptide insertion in the fingers subdomain: effect of mutations D67N and T215Y on removal of thymidine nucleotide analogues from blocked DNA prim
    Tania Matamoros
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas Universidad Autonoma de Madrid, 28049 Madrid, Spain
    J Biol Chem 279:24569-77. 2004
    ..Insertions are usually associated with thymidine analogue resistance mutations, such as T215Y...
  9. pmc Quantification of oxidative DNA lesions in tissues of Long-Evans Cinnamon rats by capillary high-performance liquid chromatography-tandem mass spectrometry coupled with stable isotope-dilution method
    Jin Wang
    Department of Chemistry, University of California, Riverside, California 92521 0403, United States
    Anal Chem 83:2201-9. 2011
    ....
  10. ncbi Telbivudine versus lamivudine in patients with chronic hepatitis B
    Ching Lung Lai
    University Department of Medicine, Queen Mary Hospital, Hong Kong, China
    N Engl J Med 357:2576-88. 2007
    ..Reducing hepatitis B virus (HBV) replication to minimal levels is emerging as a key therapeutic goal for chronic hepatitis B...
  11. ncbi Thymidine analogs are transferred from prelabeled donor to host cells in the central nervous system after transplantation: a word of caution
    Terry C Burns
    Stem Cell Institute, University of Minnesota, 420 Delaware Street, Minneapolis, Minnesota 55455, USA
    Stem Cells 24:1121-7. 2006
    b>Thymidine analogs, including bromodeoxyuridine, chlorodeoxyuridine, iododeoxyuridine, and tritiated thymidine, label dividing cells by incorporating into DNA during S phase of cell division and are widely employed to identify cells ..
  12. ncbi Heterogeneous cell renewal of pancreas in mice: [(3)H]-thymidine autoradiographic investigation
    Yasushi Magami
    Fourth Department of Internal Medicine, Tokyo Medical University, 6 7 1 Nishishinjuku, Shinjuku ku, Tokyo 160 0023, Japan
    Pancreas 24:153-60. 2002
    ..Although cell kinetics of the gastrointestinal mucosa has been extensively examined, that of the pancreas has not been fully analyzed...
  13. ncbi Synthesis and biological evaluation of bicyclic nucleosides as inhibitors of M. tuberculosis thymidylate kinase
    Ineke Van Daele
    Laboratory for Medicinal Chemistry FFW, Gent University Harelbekestraat 72, Gent, Belgium
    ChemMedChem 1:1081-90. 2006
    Herein we describe the synthesis and conformational analysis of a series of bicyclic thymidine derivatives and their evaluation as inhibitors of thymidine monophosphate kinase from Mycobacterium tuberculosis (TMPKmt), based on previously ..
  14. ncbi Radiosynthesis of 3'-deoxy-3'-[(18)F]fluorothymidine: [(18)F]FLT for imaging of cellular proliferation in vivo
    J R Grierson
    Research Imaging Laboratory, University of Washington Medical Center, Seattle, Washington 98195, USA
    Nucl Med Biol 27:143-56. 2000
    ..18)F]FLT has been designed as a new positron emission tomography imaging agent for visualizing cellular proliferation in vivo based on the metabolism of thymidine.
  15. doi 3'-[4-Aryl-(1,2,3-triazol-1-yl)]-3'-deoxythymidine analogues as potent and selective inhibitors of human mitochondrial thymidine kinase
    Sara Van Poecke
    Laboratory for Medicinal Chemistry FFW, Ghent University, Harelbekestraat 72, 9000 Gent, Belgium
    J Med Chem 53:2902-12. 2010
    ..effort to increase the potency and selectivity of earlier identified substrate-based inhibitors of mitochondrial thymidine kinase 2 (TK-2), we now describe the synthesis of new thymidine analogues containing a 4- or 5-substituted 1,2,3-..
  16. ncbi 2-[11C]thymidine positron emission tomography as an indicator of thymidylate synthase inhibition in patients treated with AG337
    Paula Wells
    Imperial College School of Medicine, Hammersmith Hospital, London, UK
    J Natl Cancer Inst 95:675-82. 2003
    Some anticancer drugs inhibit thymidylate synthase (TS), a key enzyme for thymidine nucleotide biosynthesis. Cells can compensate for depleted thymidine levels by taking up extracellular thymidine via a salvage pathway...
  17. ncbi Thymidine-phosphorothioate oligonucleotides induce activation and apoptosis of CLL cells independently of CpG motifs or BCL-2 gene interference
    J E Castro
    John and Rebecca Moores Cancer Center, University of California San Diego, La Jolla, CA, USA
    Leukemia 20:680-8. 2006
    ..Surprisingly, we found that thymidine-containing PS-ODN, but not PO-ODN, induced activation and apoptosis of CLL cells independent of BCL-2 antisense ..
  18. ncbi Telbivudine versus lamivudine in Chinese patients with chronic hepatitis B: Results at 1 year of a randomized, double-blind trial
    Jinlin Hou
    Nanfang Hospital, Guangzhou, China
    Hepatology 47:447-54. 2008
    ..Viral resistance in telbivudine recipients was approximately half that observed with lamivudine; however, this difference was not statistically significant. Clinical adverse events were similar in the two treatment groups...
  19. ncbi Thymidine kinase, thymidylate synthase, and dihydropyrimidine dehydrogenase profiles of cell lines of the National Cancer Institute's Anticancer Drug Screen
    J L Grem
    Developmental Therapeutics Department, Medicine Branch, Division of Clinical Sciences, National Cancer Institute at the National Naval Medical Center, Bethesda, Maryland 20889, USA
    Clin Cancer Res 7:999-1009. 2001
    ..EXPERIMENTAL DESIGN: Thymidine kinase (TK) activity was assessed by conversion of [(3)H]thymidine to [(3)H]TMP...
  20. ncbi [Blocking intrauterine infection by telbivudine in pregnant chronic hepatitis B patients]
    Li Ju Zhang
    Department of Obstetrics, Beijng Ditan Hospital, Beijing 100015, China
    Zhonghua Gan Zang Bing Za Zhi 17:561-3. 2009
    ..To investigate the efficacy of telbivudine on intrauterine hepatitis B virus (HBV) infection during the last stage of pregnancy...
  21. doi Randomized clinical trial: efficacy and safety of telbivudine and lamivudine in treatment-naïve patients with HBV-related decompensated cirrhosis
    H L Y Chan
    Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Hong Kong, China
    J Viral Hepat 19:732-43. 2012
    ..Telbivudine compared to lamivudine was associated with a higher rate of patients with both viral suppression and ALT normalization, a trend towards a higher rate of survival and significant improvement in glomerular filtration...
  22. doi On-treatment serum HBsAg level is predictive of sustained off-treatment virologic response to telbivudine in HBeAg-positive chronic hepatitis B patients
    Wei Cai
    Department of Infectious Diseases, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, People s Republic of China
    J Clin Virol 48:22-6. 2010
    ..Telbivudine is one of the most frequently used antiviral drug at the current stage, but its long-term effectiveness, particularly at off-treatment, is still unclear...
  23. pmc Thymidine-dependent Staphylococcus aureus small-colony variants are associated with extensive alterations in regulator and virulence gene expression profiles
    Barbara C Kahl
    Institute of Medical Microbiology, University Clinics of Muenster, Domagkstr 10, 48149 Muenster, Germany
    Infect Immun 73:4119-26. 2005
    ..b>Thymidine-dependent trimethoprim-sulfamethoxazole (SXT)-resistant S...
  24. doi 3'-(1,2,3-Triazol-1-yl)-3'-deoxythymidine analogs as substrates for human and Ureaplasma parvum thymidine kinase for structure-activity investigations
    Jay Lin
    Department of Anatomy, Physiology and Biochemistry, Veterinary Medical Biochemistry, Swedish University Agricultural Sciences, Uppsala, Sweden
    Bioorg Med Chem 18:3261-9. 2010
    ..parvum (Up) causes opportunistic infections and relies on salvage of nucleosides for DNA synthesis and Up thymidine kinase (UpTK) provides the necessary thymidine nucleotides...
  25. ncbi Suppression of multidrug-resistant HIV-1 reverse transcriptase primer unblocking activity by alpha-phosphate-modified thymidine analogues
    Tania Matamoros
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas Universidad Autonoma de Madrid, 28049 Madrid, Spain
    J Mol Biol 349:451-63. 2005
    ..the ATP-dependent phosphorolytic activity on primers terminated with alpha-boranophosphate derivatives of thymidine analogues...
  26. pmc Efficient labeling of fission yeast Schizosaccharomyces pombe with thymidine and BUdR
    Jeffrey A Hodson
    Molecular and Cell Biology Laboratory, The Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Nucleic Acids Res 31:e134. 2003
    ..The strain co-expresses the Herpes simplex virus thymidine kinase gene (hsv-tk) and a human equilibrative nucleoside transporter (hENT1)...
  27. pmc Inhibition of viral replication downregulates CD4(+)CD25(high) regulatory T cells and programmed death-ligand 1 in chronic hepatitis B
    Xue Ping Nan
    Center for Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi an, China
    Viral Immunol 25:21-8. 2012
    ..Our findings suggest that the antiviral effect of the nucleoside analogs may be attributable not only to their direct effect on virus suppression, but also to their immunoregulatory capabilities...
  28. pmc Structure-guided engineering of human thymidine kinase 2 as a positron emission tomography reporter gene for enhanced phosphorylation of non-natural thymidine analog reporter probe
    Dean O Campbell
    Department of Molecular and Medical Pharmacology, UCLA, Los Angeles, CA 90095, USA
    J Biol Chem 287:446-54. 2012
    ..Here we describe a new PRG-PRP system that employs, as the PRG, a mutated form of human thymidine kinase 2 (TK2) and 2'-deoxy-2'-18F-5-methyl-1-β-L-arabinofuranosyluracil (L-18F-FMAU) as the PRP...
  29. doi Safety evaluation of telbivudine
    David Yiu Kuen But
    University of Hong Kong, Queen Mary Hospital, Department of Medicine, Pokfulam Road, Hong Kong SAR, China
    Expert Opin Drug Saf 9:821-9. 2010
    ..5- to 6.6-log copies/ml hepatitis B DNA reduction at 12 weeks in an early viral kinetic study, a potency comparable to entecavir. It is also one of the few drugs in the treatment of CHB under FDA pregnancy Category B...
  30. doi Synthesis and inhibitory activity of thymidine analogues targeting Mycobacterium tuberculosis thymidine monophosphate kinase
    Sara Van Poecke
    Laboratory for Medicinal Chemistry, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, B 9000 Ghent, Belgium
    Bioorg Med Chem 19:7603-11. 2011
    We report on Mycobacterium tuberculosis thymidine monophosphate kinase (TMPKmt) inhibitory activities of a series of new 3'- and 5'-modified thymidine analogues including α- and β-derivatives...
  31. pmc Phenotypic characterization of drug resistance-associated mutations in HIV-1 RT connection and RNase H domains and their correlation with thymidine analogue mutations
    Renan B Lengruber
    Departamento de Genetica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
    J Antimicrob Chemother 66:702-8. 2011
    ..In this work, we have characterized the correlation of recently described C-terminal domain mutations with thymidine analogue mutations (TAMs), as well as their phenotypic impact on susceptibility to zidovudine and nevirapine.
  32. pmc Dependence of substrate binding and catalysis on pH, ionic strength, and temperature for thymine DNA glycosylase: Insights into recognition and processing of G·T mispairs
    Atanu Maiti
    Department of Biochemistry and Molecular Biology, Greenebaum Cancer Center, School of Medicine, University of Maryland, Baltimore, 21201, USA
    DNA Repair (Amst) 10:545-53. 2011
    ..Our findings provide important insight into catalysis by TDG, particularly for mutagenic G·T mispairs...
  33. ncbi In vivo labeling of fission yeast DNA with thymidine and thymidine analogs
    Sasirekha Sivakumar
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Methods 33:213-9. 2004
    In vivo labeling of DNA with thymidine and thymidine analogs has long been a cornerstone of replication studies. Unfortunately, yeast lack a thymidine salvage pathway and thus do not incorporate exogenous thymidine...
  34. pmc Thiothymidine plus low-dose UVA kills hyperproliferative human skin cells independently of their human papilloma virus status
    Olivier Reelfs
    Cancer Research UK, Institute for Cell and Molecular Science, Skin Tumour Laboratory, 4, Newark Street, London E1 2AT, United Kingdom
    Mol Cancer Ther 6:2487-95. 2007
    The thymidine analogue 4-thiothymidine (S(4)TdR) is a photosensitizer for UVA radiation...
  35. pmc Thymidine analog methods for studies of adult neurogenesis are not equally sensitive
    Benedetta Leuner
    Department of Psychology, Neuroscience Institute, Princeton University, Princeton, New Jersey 08544, USA
    J Comp Neurol 517:123-33. 2009
    Adult neurogenesis is often studied by labeling new cells with the thymidine analog bromodeoxyuridine (BrdU) and using immunohistochemical methods for their visualization...
  36. pmc In vitro activity of pyrvinium pamoate against Entamoeba histolytica and Giardia intestinalis using radiolabelled thymidine incorporation and an SYBR Green I-based fluorescence assay
    Autumn S Downey
    Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA
    J Antimicrob Chemother 64:751-4. 2009
    ..To assess the in vitro activity of the FDA-approved antihelminthic drug pyrvinium pamoate against Entamoeba histolytica and Giardia intestinalis...
  37. ncbi Effects of 5-hydroxymethyluracil and 3-aminobenzamide on the repair and toxicity of 5-hydroxymethyl-2'-deoxyuridine in mammalian cells
    R J Boorstein
    Department of Pathology, New York University School of Medicine, New York 10016
    Cancer Res 49:1509-14. 1989
    5-Hydroxymethyl-2'-deoxyuridine (HmdUrd), a cytotoxic analogue of thymidine, has been proposed for use as an anticancer agent. HmdUrd is incorporated into DNA and then removed at a rate of 30-40% per day...
  38. doi EdU incorporation is an alternative non-radioactive assay to [(3)H]thymidine uptake for in vitro measurement of mice T-cell proliferations
    Yongmao Yu
    The Division of Allergy and Clinical Immunology and Developmental and Stem Cell Biology Program, The Hospital for Sick Children, 555 University Ave, Toronto, Ontario M5G 1X8, Canada
    J Immunol Methods 350:29-35. 2009
    T lymphocyte proliferations can be measured by [(3)H]thymidine incorporation. However, many labs avoid this technique because of the need to use radioactive substrates...
  39. ncbi Diminished selection for thymidine-analog mutations associated with the presence of M184V in Ethiopian children infected with HIV subtype C receiving lamivudine-containing therapy
    Diana Averbuch
    Hadassah University Medical Center, Jerusalem, Israel
    Pediatr Infect Dis J 25:1049-56. 2006
    We retrospectively studied the effect of the lamivudine-induced reverse transcription mutation M184V on selection of thymidine analog mutations (TAMs) in HIV subtype C-infected children and on clinical outcome.
  40. ncbi Embryonic folate metabolism and mouse neural tube defects
    A Fleming
    Neural Development Unit, Institute of Child Health, University College London, London WC1N 1EH, UK
    Science 280:2107-9. 1998
    ..Excessive incorporation of [3H]thymidine in splotch embryos indicates a metabolic deficiency in the supply of folate for the biosynthesis of pyrimidine...
  41. doi High-throughput hacking of the methylation patterns in breast cancer by in vitro transcription and thymidine-specific cleavage mass array on MALDI-TOF silico-chip
    Ramin Radpour
    Laboratory for Prenatal Medicine and Gynecologic Oncology, Women s Hospital Department of Biomedicine, University of Basel, Basel, Switzerland
    Mol Cancer Res 6:1702-9. 2008
    ..we developed a method that allows for the simultaneous detection of multiple target CpG residues by using thymidine-specific cleavage mass array on matrix-assisted laser desorption/ionization time-of-flight silicon chips...
  42. pmc The use of thymidine analogs to improve the replication of an extra DNA base pair: a synthetic biological system
    A Michael Sismour
    Department of Chemistry, University of Florida, Gainesville, FL 32611 7200, USA
    Nucleic Acids Res 33:5640-6. 2005
    ..Reported here is a solution to this problem. The solution replaces thymidine triphosphate by 2-thiothymidine triphosphate (2-thioTTP)...
  43. ncbi Mitochondrial DNA depletion and thymidine phosphate pool dynamics in a cellular model of mitochondrial neurogastrointestinal encephalomyopathy
    Giovanna Pontarin
    Department of Biology, University of Padova, I 35131 Padova, Italy
    J Biol Chem 281:22720-8. 2006
    ..Patients lack a functional thymidine phosphorylase and their plasma contains high concentrations of thymidine and deoxyuridine; elevation of the ..
  44. ncbi Inhibition of mitogen-activated protein kinase kinase enhances apoptosis induced by arsenic trioxide in human breast cancer MCF-7 cells
    Jian Ye
    Department of Molecular Cell Biology and Toxicology, Jiangsu Provincial Key Laboratory of Human Functional Genomics and Applied Toxicology, School of Public Health, Nanjing Medical University, Nanjing, PR China
    Clin Exp Pharmacol Physiol 32:1042-8. 2005
    ..cells as determined by the 3-(4,5-dimethyl-2 thiazoyl)-2,5-diphenyl-2H-tetrazolium bromide assay and [Methyl-3H]-thymidine incorporation...
  45. doi Telbivudine for the management of chronic hepatitis B virus infection
    S James Matthews
    Division of Clinical Pharmacy, Department of Pharmacy Practice, School of Pharmacy, Bouve College of Health Sciences, Northeastern University, 237 Mugar Hall, 360 Huntington Avenue, Boston, MA 02115, USA
    Clin Ther 29:2635-53. 2007
    ....
  46. pmc UNG-initiated base excision repair is the major repair route for 5-fluorouracil in DNA, but 5-fluorouracil cytotoxicity depends mainly on RNA incorporation
    Henrik Sahlin Pettersen
    Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, N 7489 Trondheim, Norway
    Nucleic Acids Res 39:8430-44. 2011
    ..In conclusion, UNG-initiated BER is the major route for FU-DNA repair, but cytotoxicity of FU is predominantly RNA-mediated, while DNA-mediated effects are limited to FdUrd...
  47. doi Unchanged thymidine triphosphate pools and thymidine metabolism in two lines of thymidine kinase 2-mutated fibroblasts
    Miriam Frangini
    Department of Biology, University of Padova, Italy
    FEBS J 276:1104-13. 2009
    Mitochondrial thymidine kinase (TK2) catalyzes the phosphorylation of thymidine in mitochondria...
  48. doi High fidelity of base pairing by 2-selenothymidine in DNA
    Abdalla E A Hassan
    Department of Chemistry, Georgia State University, Atlanta, Georgia 30303, USA
    J Am Chem Soc 132:2120-1. 2010
    ..Via the selenium atomic probing at the 2-position of thymidine, we have investigated the wobble discrimination by manipulating the steric and electronic effects at the 2-exo ..
  49. pmc Thymidine kinase 1 as a molecular target for boron neutron capture therapy of brain tumors
    Rolf F Barth
    Department of Pathology, Division of Biostatistics, Ohio State University, Columbus, OH 43210, USA
    Proc Natl Acad Sci U S A 105:17493-7. 2008
    The purpose of the present study was to evaluate the effectiveness of a 3-carboranyl thymidine analogue (3CTA), 3-[5-{2-(2,3-dihydroxyprop-1-yl)-o-carboran-1-yl}pentan-1-yl] thymidine, designated N5-2OH, for boron neutron capture therapy ..
  50. ncbi Yersiniophage phiR1-37 is a tailed bacteriophage having a 270 kb DNA genome with thymidine replaced by deoxyuridine
    Saija Kiljunen
    Department of Medical Biochemistry and Molecular Biology, Institute of Biomedicine, University of Turku, Turku, Finland
    Microbiology 151:4093-102. 2005
    ..was subjected to HPLC-MS/MS analysis, which demonstrated that the phiR1-37 genome is composed of DNA in which thymidine (T) is >99 % replaced by deoxyuridine (dU)...
  51. doi Longitudinal effects of thymidine analogues on mtDNA, mtRNA and multidrug resistance (MDR-1) induction in cultured cells
    Eszter Papp
    Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, Canada
    J Antimicrob Chemother 61:1048-52. 2008
    ..Our aim was to investigate the longitudinal effects of two thymidine analogue NRTIs, zidovudine and stavudine, on human cells, measuring their effects on the levels of mtDNA, mtRNA ..
  52. doi Effect of telbivudine therapy on the cellular immune response in chronic hepatitis B
    Yu Chen
    State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, School of Medicine, Zhejiang University, Qingchun Road, Hangzhou, PR China
    Antiviral Res 91:23-31. 2011
    ..Enhanced HBV-specific T-cell reactivity to telbivudine therapy, which peaked at treatment week 12, was confined to a subgroup of effective-treated patients who achieved greater viral suppression...
  53. pmc N348I in reverse transcriptase provides a genetic pathway for HIV-1 to select thymidine analogue mutations and mutations antagonistic to thymidine analogue mutations
    Jessica Radzio
    Department of Medicine, University of Pittsburgh School of Medicine, PA, USA
    AIDS 24:659-67. 2010
    ..g. L74V and M184V) resistance mutations in HIV-1 reverse transcriptase are antagonistic toward thymidine analogue mutations (TAMs) that confer zidovudine (ZDV) resistance...
  54. doi Auger electron emitter against multiple myeloma--targeted endo-radio-therapy with 125I-labeled thymidine analogue 5-iodo-4'-thio-2'-deoxyuridine
    Agnieszka Morgenroth
    Nuclear Medicine Clinic, University Ulm, Albert Einstein Allee 23, D 89081 Ulm, Germany
    Nucl Med Biol 38:1067-77. 2011
    ..Here we evaluated the antimyeloma potential of the Auger electron-emitting thymidine analogue (125)I-labeled 5-iodo-4'-thio-2'-deoxyuridine ([(125)I]ITdU).
  55. ncbi Renewal of the rabbit corneal epithelium as investigated by autoradiography after intravitreal injection of 3H-thymidine
    A Haddad
    Departamento de Morfologia, Faculdade de Medicina, USP, Ribeirao Preto SP, Brasil
    Cornea 19:378-83. 2000
    ..To evaluate the renewal time of the rabbit corneal epithelium and to verify whether there are differences in the rate of renewal related to the topography of the cornea...
  56. doi Evaluation of biomarkers of exposure and potential harm in smokers, former smokers and never-smokers
    Frazer J Lowe
    Research and Development Centre, British American Tobacco Investments Ltd, Millbrook, Southampton, Hampshire, UK
    Clin Chem Lab Med 47:311-20. 2009
    ..The objective of this study was to obtain baseline data on biomarkers of exposure (BoE) and biomarkers of potential harm (BoPH) in smokers, former smokers and never-smokers...
  57. doi Preclinical evaluation of a novel pyrimidopyrimidine for the prevention of nucleoside and nucleobase reversal of antifolate cytotoxicity
    Huw D Thomas
    Northern Institute for Cancer Research, Medical School, Newcastle University, Newcastle upon Tyne, UK
    Mol Cancer Ther 8:1828-37. 2009
    ..has equivalent potency to dipyridamole, remains active in the presence of physiologic levels of AGP, inhibits thymidine incorporation into DNA, and prevents thymidine and hypoxanthine rescue from the multitargeted antifolate, ..
  58. ncbi High-throughput evaluation of antioxidant and pro-oxidant activities of polyphenols with thymidine protection assays
    Stéphane Meunier
    Service de Marquage Moléculaire et de Chimie Bio Organique, DBJC DSV, CEA Saclay, 91191 Gif sur Yvette Cedex, France
    Chembiochem 6:1234-41. 2005
    ..immunoassay, a triple-screening procedure was used to evaluate the ability of different compounds to protect thymidine under different oxidative stresses...
  59. ncbi Impact of HIV-1 reverse transcriptase polymorphism F214L on virological response to thymidine analogue-based regimens in antiretroviral therapy (ART)-naive and ART-experienced patients
    Francesca Ceccherini-Silberstein
    Department of Experimental Medicine, University of Rome Tor Vergata, Rome, Italy
    J Infect Dis 196:1180-90. 2007
    A negative association between the polymorphism F214L and type 1 thymidine analogue (TA) mutations (TAMs) has been observed. However, the virological response to TAs according to the detection of F214L has not been evaluated.
  60. ncbi Thymidine analogue mutation profiles: factors associated with acquiring specific profiles and their impact on the virological response to therapy
    Alessandro Cozzi-Lepri
    Royal Free and University College Medical School, London, UK
    Antivir Ther 10:791-802. 2005
    Studies have suggested that HIV-1 may develop thymidine analogue mutations (TAMs) by one of two distinct pathways - the TAM1 pathway (including mutations 41L, 210W and 215Y) or the TAM2 pathway (including mutations 67N, 70R and 219E/Q) - ..
  61. ncbi Evaluation of human thymidine kinase 1 substrates as new candidates for boron neutron capture therapy
    Ashraf S Al-Madhoun
    Department of Molecular Biosciences, Division of Veterinary Medical Biochemistry, Swedish University of Agricultural Sciences, Biomedical Center, Uppsala, Sweden
    Cancer Res 64:6280-6. 2004
    b>Thymidine analogs containing o-carboranylalkyl groups at the 3-position were screened as potential substrates for human thymidine kinase 1 (TK1), an enzyme that is selectively expressed in a variety of rapidly proliferating cells, ..
  62. ncbi The role of thymidine phosphorylase, an angiogenic enzyme, in tumor progression
    Shin ichi Akiyama
    Department of Molecular Oncology, Course of Advanced Therapeutics, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890 8544
    Cancer Sci 95:851-7. 2004
    b>Thymidine phosphorylase (TP), an enzyme involved in pyrimidine metabolism, is identical with an angiogenic factor, platelet-derived endothelial cell growth factor (PD-ECGF)...
  63. pmc The K65R mutation in human immunodeficiency virus type 1 reverse transcriptase exhibits bidirectional phenotypic antagonism with thymidine analog mutations
    Urvi M Parikh
    Division of Infectious Diseases, Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    J Virol 80:4971-7. 2006
    ..insight, we examined trends in the frequency of K65R in a large genotype database, the association of K65R with thymidine analog mutations (TAMs) and other NRTI mutations, and the viral susceptibility profile of HIV-1 with K65R alone ..
  64. ncbi Biophysical characterization of vaccinia virus thymidine kinase substrate utilization
    Robert F Smith
    Department of Chemistry and Biochemistry, Northern Arizona University, Flagstaff, AZ 86011 5698, USA
    J Virol Methods 142:151-8. 2007
    ..of the kinetics and thermodynamics that underlie substrate utilization reactions of vaccinia virus thymidine kinase (VVTK) has been undertaken...
  65. doi Synthesis and crystallographic analysis of 5-Se-thymidine DNAs
    Abdalla E A Hassan
    Department of Chemistry, Georgia State University, Atlanta, Georgia 30303, USA
    Org Lett 11:2503-6. 2009
    We investigated the possibility of the interaction of 5-CH(3) of thymidine and its 5'-phosphate backbone (C-H.....
  66. ncbi Bromovinyl-deoxyuridine: A selective substrate for mitochondrial thymidine kinase in cell extracts
    Elisa Franzolin
    Department of Biology, University of Padova, 35131 Padova, Italy
    Biochem Biophys Res Commun 344:30-6. 2006
    Cellular models of mitochondrial thymidine kinase (TK2) deficiency require a reliable method to measure TK2 activity in whole cell extracts containing two interfering deoxyribonucleoside kinases, thymidine kinase 1 (TK1) and ..
  67. ncbi Effect of thiazole orange doubly labeled thymidine on DNA duplex formation
    Yasumasa Kimura
    RIKEN Omics Science Center OSC, RIKEN Yokohama Institute, 1 7 22 Suehiro cho, Yokohama, Kanagawa 230 0045, Japan
    Biochemistry 51:6056-67. 2012
    ..fluorescent oligonucleotides (ECHOs) were developed as uniquely labeled DNA oligomers containing commonly one thymidine having two covalently linked thiazole orange dye moieties...
  68. ncbi Neuron loss in the mouse hippocampus following prenatal injection of tritiated thymidine or saline
    C Schmitz
    Department of Anatomy and Cell Biology, RWTH University Aachen, Germany
    Int J Dev Neurosci 17:185-90. 1999
    To investigate possible effects of injections of tritiated thymidine ([3H]dThd) into pregnant mice or the injection procedure itself on the proliferation of neuronal precursor cells in the fetuses, pregnant mice received intraperitoneal ..
  69. doi On-treatment monitoring of chronic hepatitis B virus infection: an Asian-Pacific perspective
    Rong Nan Chien
    Liver Research Unit, Chang Gung Memorial Hospital and University, Keelung, Taiwan
    J Gastroenterol Hepatol 25:852-7. 2010
    ....
  70. doi High NADPH/NADP+ ratio improves thymidine production by a metabolically engineered Escherichia coli strain
    Hyeon Cheol Lee
    BioNgene Co, Ltd 10 1, 1Ka, Myungryun Dong, Jongro Ku, Seoul 110 521, Republic of Korea
    J Biotechnol 149:24-32. 2010
    b>Thymidine is a commercially important precursor in the production of antiviral drugs, including azidothymidine for the treatment of AIDS...
  71. ncbi Uptake of positron emission tomography tracers in experimental bacterial infections: a comparative biodistribution study of radiolabeled FDG, thymidine, L-methionine, 67Ga-citrate, and 125I-HSA
    Y Sugawara
    Division of Nuclear Medicine, Department of Internal Medicine, The University of Michigan Medical Center, Ann Arbor, Michigan 48109 0028, USA
    Eur J Nucl Med 26:333-41. 1999
    ..to evaluate the localization of positron emission tomography (PET) tracers [2-deoxy-2-fluoro-D-glucose (FDG), thymidine, and L-methionine] in sites of bacterial infection, and to contrast this with that of other tracers...
  72. ncbi Heavy chain ferritin enhances serine hydroxymethyltransferase expression and de novo thymidine biosynthesis
    E W Oppenheim
    Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853, USA
    J Biol Chem 276:19855-61. 2001
    ..Our data suggest that the iron regulatory pool may play an important role in regulating folate metabolism and thereby thymidine biosynthesis.
  73. doi Human RECQL5 overcomes thymidine-induced replication stress
    Rachel Blundred
    The Institute for Cancer Studies, University of Sheffield, Sheffield, UK
    DNA Repair (Amst) 9:964-75. 2010
    ..We demonstrate that overexpressed RECQL5 promotes survival during thymidine-induced slowing of replication forks in human cells...
  74. pmc Effects of platelet-derived endothelial cell growth factor/thymidine phosphorylase, substrate, and products in a three-dimensional model of angiogenesis
    D P Stevenson
    Diagnostics Research Unit, Department of Obstetrics and Gynecology, King s College School of Medicine and Dentistry, London, United Kingdom
    Am J Pathol 152:1641-6. 1998
    Platelet-derived endothelial cell growth factor/thymidine phosphorylase (PD-ECGF/TP) is associated with angiogenesis and the progression of human breast and ovarian cancers...
  75. pmc A two-stage one-pot enzymatic synthesis of TDP-L-mycarose from thymidine and glucose-1-phosphate
    Haruko Takahashi
    Division of Medicinal Chemistry, College of Pharmacy and Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, Texas 78712, USA
    J Am Chem Soc 128:1432-3. 2006
    This report describes a procedure combining six enzymes native to Escherichia coli or Salmonella typhi, such as thymidine kinase (TK), thymidylate kinase (TMK), nucleoside diphosphate kinase (NDK), pyruvate kinase (PK; for ATP ..
  76. doi Accumulation of thymidine-derived sugars in thymidine phosphorylase overexpressing cells
    I V Bijnsdorp
    VU University Medical Center, Department Medical Oncology, De Boelelaan 1117 CCA 1 42, 1081 HV Amsterdam, The Netherlands
    Biochem Pharmacol 80:786-92. 2010
    b>Thymidine phosphorylase (TP) is often overexpressed in cancer and potentially plays a role in the stimulation of angiogenesis...
  77. doi Characterization of adducts formed in reactions of acrolein with thymidine and calf thymus DNA
    Agnieszka J Pawłowicz
    Laboratory of Organic Chemistry, Abo Akademi University, Biskopsgatan 8, FIN 20500 Turku Abo
    Chem Biodivers 5:177-88. 2008
    ..In this study, we examined the reactivity of acrolein towards thymidine and calf-thymus double- and single-stranded DNA in aqueous buffered solutions...
  78. pmc DNA tandem lesion repair by strand displacement synthesis and nucleotide excision repair
    Shuhei Imoto
    Department of Chemistry, Johns Hopkins University, 3400 North Charles Street, Baltimore, Maryland 21218, USA
    Biochemistry 47:4306-16. 2008
    ..These data reveal two solutions that DNA repair systems can use to counteract the formation of tandem lesions...
  79. pmc Thymidine-dependent Staphylococcus aureus small-colony variants: human pathogens that are relevant not only in cases of cystic fibrosis lung disease
    Silke Besier
    Institute of Medical Microbiology and Infection Control, Hospital of Johann Wolfgang Goethe University, Frankfurt Main, Paul Ehrlich Strasse 40, 60596 Frankfurt Main, Germany
    J Clin Microbiol 46:3829-32. 2008
    We report the isolation of thymidine-dependent small-colony variants (TD-SCVs) of Staphylococcus aureus from unusual infection sites of patients with chronic soft tissue infection, tympanitis, bronchitis, peritonitis, and septicemia...
  80. ncbi Hyperglycaemia inhibits thymidine incorporation and cell growth via protein kinase C, mitogen-activated protein kinases and nitric oxide in human umbilical vein endothelium
    Susana Rojas
    Cellular and Molecular Physiology Laboratory, Department of Physiology, Faculty of Biological Sciences, University of Concepcion, PO Box 160 C, Concepcion, Chile
    Exp Physiol 88:209-19. 2003
    ..e. hyperglycaemia) inhibits cell proliferation and incorporation of the endogenous nucleoside thymidine into DNA in human umbilical vein endothelial cells (HUVECs). Cells in their log-phase of growth (3.7 +/- 0...
  81. ncbi Reaction of glyoxal with 2'-deoxyguanosine, 2'-deoxyadenosine, 2'-deoxycytidine, cytidine, thymidine, and calf thymus DNA: identification of DNA adducts
    Raymond Olsen
    National Institute of Occupational Health, P O Box 8149 Dep, N 0033 Oslo, Norway
    Chem Res Toxicol 18:730-9. 2005
    ..In this study, the reactions of glyoxal with 2'-deoxyguanosine, 2'-deoxyadenosine, 2'-deoxycytidine, cytidine, thymidine, and calf thymus DNA have been studied in aqueous buffered solutions...
  82. doi Increased migration by stimulation of thymidine phosphorylase in endothelial cells of different origin
    I V Bijnsdorp
    Department of Medical Oncology, VU University Medical Center, Amsterdam, The Netherlands
    Nucleosides Nucleotides Nucleic Acids 29:482-7. 2010
    b>Thymidine phosphorylase (TP) catalyzes the phosphorylytic cleavage of thymidine to thymine and deoxyribose-1-phosphate. The latter may be involved in the angiogenic stimulation of TP...
  83. pmc Multiple mutant of Escherichia coli synthesizing virtually thymineless DNA during limited growth
    H H El-Hajj
    Department of Pathology, University of Michigan Medical School, Ann Arbor 48109 0602
    J Bacteriol 174:4450-6. 1992
    ..ung, which produces uracil-DNA N-glycosylase, a repair enzyme that removes uracil from DNA; deoA, which produces thymidine (deoxyuridine) phosphorylase, which would degrade exogenous deoxyuridine; and thyA, which produces thymidylate ..
  84. pmc Thiothymidine combined with UVA as a potential novel therapy for bladder cancer
    S W Pridgeon
    Northern Institute for Cancer Research, Medical School, Newcastle University, Newcastle NE2 4HH, UK
    Br J Cancer 104:1869-76. 2011
    ..Studies were performed to determine if the combination of S(4)TdR and UVA could be an effective treatment for bladder cancer...
  85. ncbi Therapeutic potential of the dual-targeted TAS-102 formulation in the treatment of gastrointestinal malignancies
    Olaf H Temmink
    Department of Medical Oncology, VU University Medical Center, Amsterdam 1007 MB, The Netherlands
    Cancer Sci 98:779-89. 2007
    ..The thymidine analog trifluorothymidine (TFT) has been shown to bypass resistance pathways for 5FU derivatives (S-1, UFT, ..
  86. pmc Generation of guanine-thymidine cross-links in DNA by peroxynitrite/carbon dioxide
    Byeong Hwa Yun
    Division of Environmental Health Sciences, Wadsworth Center, NYS Department of Health, Empire State Plaza, P O Box 509, Albany, New York 12201 0509, USA
    Chem Res Toxicol 24:1144-52. 2011
    ..002% each) were found. It is shown that the formation of d(G*pT*) enzyme digestion product can arise only from intrastrand cross-links, whereas d(G*-T*) can arise from both interstrand and intrastrand cross-linked products...
  87. ncbi Thymidine phosphorylase gene mutations in MNGIE, a human mitochondrial disorder
    I Nishino
    Columbia University College of Physicians and Surgeons, Department of Neurology, 630 West 168 Street, P and S 4 443, New York, NY 10032, USA
    Science 283:689-92. 1999
    ..Examination of 12 MNGIE probands revealed homozygous or compound-heterozygous mutations in the gene specifying thymidine phosphorylase (TP), located on chromosome 22q13.32-qter...
  88. pmc Isolation from fetal bovine serum of an apolipoprotein-H-like protein which inhibits thymidine incorporation in fetal calf erythroid cells
    Q G Li
    Endocrine Laboratory, Royal Victoria Hospital, Montreal, Canada
    Biochem J 267:261-4. 1990
    A 46 kDa heparin-binding protein which inhibits thymidine incorporation in cultures of fetal calf liver erythroid cells was isolated from fetal bovine serum by affinity chromatography on heparin-Sepharose, ion-exchange chromatography, ..
  89. doi L-leucine increases [3H]-thymidine incorporation in chicken hepatocytes: involvement of the PKC, PI3K/Akt, ERK1/2, and mTOR signaling pathways
    Min Young Lee
    Department of Veterinary Physiology, College of Veterinary Medicine, Biotherapy Human Resources Center BK21, Chonnam National University, Gwangju 500 757, Korea
    J Cell Biochem 105:1410-9. 2008
    ..Phospholipase C (PLC) and protein kinase C (PKC) mediated the L-leucine-induced increases in [3H]-thymidine incorporation and cyclin D1/CDK4 and cyclin E/CDK2 expression, as U73122 (a PLC inhibitor) or ..
  90. pmc Rosiglitazone improves lipoatrophy in patients receiving thymidine-sparing regimens
    Marisa Tungsiripat
    Cleveland Clinic, Ohio, USA
    AIDS 24:1291-8. 2010
    b>Thymidine reverse transcriptase inhibitors (tNRTI) are strong inhibitors of PPAR-gamma and clearly implicated as a cause of lipoatrophy...
  91. ncbi Thymidine analogue mutations in antiretroviral-naive HIV-1 patients on triple therapy including either zidovudine or stavudine
    Laurence Bocket
    Service Universitaire de Virologie, Centre Hospitalier Regional de Lille, Lille, France
    J Antimicrob Chemother 53:89-94. 2004
    The aims of this study were to: (i) determine the incidence of thymidine-associated mutations (TAMs) in an observational clinical cohort of naive HIV-1 patients who stopped first-line therapy including either zidovudine or stavudine; and ..
  92. ncbi O-[18F]fluoromethyl-L-tyrosine is a potential tracer for monitoring tumour response to chemotherapy using PET: an initial comparative in vivo study with deoxyglucose and thymidine
    Gengo Yamaura
    Department of Clinical Oncology, Institute of Development, Aging and Cancer, Tohoku University, 4 1 Seiryo machi, Aoba Ward, Sendai 980 8575, Japan
    Eur J Nucl Med Mol Imaging 33:1134-9. 2006
    To compare the utility of a new artificial amino acid, O-[18F]fluoromethyl-L-tyrosine ([18F]FMT), for monitoring cancer chemotherapy with deoxyglucose and thymidine.
  93. pmc Glucarpidase, leucovorin, and thymidine for high-dose methotrexate-induced renal dysfunction: clinical and pharmacologic factors affecting outcome
    Brigitte C Widemann
    Pediatric Oncology Branch, National Cancer Institute, Bethesda, MD 20892 1101, USA
    J Clin Oncol 28:3979-86. 2010
    To assess the role of the recombinant bacterial enzyme, glucarpidase (carboxypeptidase-G(2)), leucovorin, and thymidine in the management and outcome of patients with high-dose methotrexate (HDMTX) -induced nephrotoxicity.
  94. pmc Two thymidine hydroxylases differentially regulate the formation of glucosylated DNA at regions flanking polymerase II polycistronic transcription units throughout the genome of Trypanosoma brucei
    Laura J Cliffe
    Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, USA
    Nucleic Acids Res 38:3923-35. 2010
    ..We have previously characterized two thymidine-hydroxylases (TH), JBP1 and JBP2, which regulate J-biosynthesis...
  95. doi A spectroscopic investigation into the interactions of 3'-O-carboxy esters of thymidine with bovine serum albumin
    Kalyan Sundar Ghosh
    Department of Chemistry, Indian Institute of Technology, Kharagpur 721 302, India
    Biopolymers 91:737-44. 2009
    Binding studies of 3'-O-carboxy esters of thymidine, reported inhibitors of ribonucleases, with bovine serum albumin (BSA) have been explored in this report...
  96. ncbi Mispair-aligned N3T-alkyl-N3T interstrand cross-linked DNA: synthesis and characterization of duplexes with interstrand cross-links of variable lengths
    Christopher J Wilds
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA
    J Am Chem Soc 126:9257-65. 2004
    ..alkyl interstrand cross-links, we have synthesized a cross-link that bridges the N(3) positions of a mismatched thymidine base pair...
  97. pmc Incorporation of thymidine analogs for studying replication kinetics in fission yeast
    Nicholas Rhind
    Biochemistry and Molecular Pharmacology Department, University of Massachusetts Medical School, Worcester, MA, USA
    Methods Mol Biol 521:509-15. 2009
    Labeling DNA during in vivo replication by the incorporation of exogenous thymidine and thymidine analogs has been a mainstay of DNA replication and repair studies for decades...
  98. ncbi Discovery of bicyclic thymidine analogues as selective and high-affinity inhibitors of Mycobacterium tuberculosis thymidine monophosphate kinase
    Veerle Vanheusden
    Laboratory for Medicinal Chemistry FFW, Ghent University, Harelbekestraat 72, 9000 Ghent, Belgium
    J Med Chem 47:6187-94. 2004
    b>Thymidine monophosphate kinase of Mycobacterium tuberculosis (TMPKmt) represents an attractive target for selectively blocking bacterial DNA synthesis...
  99. doi Thymidine selectively enhances growth suppressive effects of camptothecin/irinotecan in MSI+ cells and tumors containing a mutation of MRE11
    Rene Rodriguez
    Institute for Cancer Studies, University of Sheffield, School of Medicine and Biomedical Sciences, Sheffield, United Kingdom
    Clin Cancer Res 14:5476-83. 2008
    ..Here, we attempt to exploit the altered response of mismatch repair (MMR)-deficient colon cancer cells and tumors to camptothecin or irinotecan and thymidine by combining them to improve therapeutic response.
  100. pmc The thymidine-dependent small-colony-variant phenotype is associated with hypermutability and antibiotic resistance in clinical Staphylococcus aureus isolates
    Silke Besier
    Institute of Medical Microbiology and Infection Control, University Hospital of Frankfurt am Main, Paul Ehrlich Strasse 40, 60596 Frankfurt am Main, Germany
    Antimicrob Agents Chemother 52:2183-9. 2008
    b>Thymidine-dependent small-colony variants (TD-SCVs) of Staphylococcus aureus can be isolated from the airway secretions of patients suffering from cystic fibrosis (CF) and are implicated in persistent and treatment-resistant infections...
  101. ncbi Rational design of 5'-thiourea-substituted alpha-thymidine analogues as thymidine monophosphate kinase inhibitors capable of inhibiting mycobacterial growth
    Ineke Van Daele
    Laboratory for Medicinal Chemistry FFW, University of Gent, Harelbekestraat 72, 9000 Ghent, Belgium
    J Med Chem 50:5281-92. 2007
    Recently, thymidine monophosphate kinase (TMPK) emerged as an attractive target for developing inhibitors of Mycobacterium tuberculosis growth. The elucidation of the X-ray structure of TMPK of M...

Research Grants81

  1. CSF-1 in Dental Biology
    SHERRY ABBOUD WERNER; Fiscal Year: 2007
    ..In op/op mice, a thymidine insertion in the coding sequence of the CSF-1 gene results in a deficiency of both the soluble (s)and membrane ..
  2. Enhancing Suicide Gene Therapy Through Mechanism-Based Approaches
    Donna S Shewach; Fiscal Year: 2010
    ..We have focused on the herpes simplex virus thymidine kinase (HSV-TK), the initial activator of the antiviral drug ganciclovir (GCV) to its cytotoxic triphosphate, ..
  3. OCULAR HERPES SIMPLEX
    Herbert Kaufman; Fiscal Year: 1993
    ..We will study thymidine kinase selective and DNA polymerase selective drugs, alone and in combination, to treat herpes infections of the ..
  4. Metabolism and toxicity of nucleoside reverse transcriptase inhibitors in non-rep
    Edward E McKee; Fiscal Year: 2010
    ..polymerase leading to mtDNA depletion;or for at least one NRTI, AZT, toxicity may be caused by the inhibition of thymidine phosphorylation with alterations of the dNTP precursor pools supporting mtDNA replication...
  5. Clinical and Immunologic Evaluation of ProstAtak for Prostate Cancer
    Estuardo Aguilar Cordova; Fiscal Year: 2007
    ..ProstAtak(tm) is a biologic drug composed of an adenoviral vector with the Herpes thymidine-kinase gene (AdV-tk) formulated for prostate delivery followed by an oral antiherpetic prodrug...
  6. Imaging Infused CD19 Specific T Cells in the Tumor
    LAURENCE COOPER; Fiscal Year: 2007
    ..Co-expression of thymidine kinase (TK) permits in vivo conditional ganciclovir (GCV)-mediated ablation in event of serious toxicity...
  7. CYTOTOXICITY AND BYSTANDER KILLING FOR HSV-TK SUBSTRATES
    Donna Shewach; Fiscal Year: 2000
    DESCRIPTION: (Applicant's Abstract) Transfer of cDNA for the herpes simplex virus thymidine kinase HSV-TK) into tumor cells allows activation of ganciclovir (GCV) to a cytotoxic nucleotide...
  8. Mitochondrial Toxicity of Antiviral Nucleosides
    Edward E McKee; Fiscal Year: 2010
    ..In recent work form our laboratory we have shown that AZT is a potent inhibitor of thymidine phosphorylation in isolated heart, liver, and brain mitochondria, and in the isolated perfused heart...
  9. DNA REPAIR IN A HORMONE RESPONSIVE GENE
    Michael Smerdon; Fiscal Year: 1999
    ..to RNA polymerase II (polII) expression will be examined in mouse cells containing the herpes simplex virus thymidine kinase (tk) gene fused to the mouse mammary tumor virus long terminal repeat (LTR)...
  10. Boronated nucleosides for neutron capture therapy of brain tumors
    Werner Tjarks; Fiscal Year: 2010
    ..Subject of the present grant application is the continued development of 3-carboranyl thymidine analogues (3CTAs), a novel class of BNCT agents for the treatment of GBM...
  11. Phase II SBIR: WHI-07: A Novel Dual-Function Microbicide
    OSMOND D CRUZ; Fiscal Year: 2005
    ..WHI-07 was rationally designed to bypass the thymidine kinase dependency of ZDV activation in genital tract secretions...
  12. Enhancing Suicide Gene Therapy Through Mechanism-Based Approaches
    Donna Shewach; Fiscal Year: 2007
    ..We have focused on the herpes simplex virus thymidine kinase (HSV-TK), the initial activator of the antiviral drug ganciclovir (GCV) to its cytotoxic triphosphate, ..
  13. CSF1 GENE EXPRESSION IN OSTEOCLAST BIOLOGY
    SHERRY ABBOUD WERNER; Fiscal Year: 2002
    ..In the op/op mouse model, a thymidine insertion in the coding osteopetrosis...
  14. Gene Therapy for Retinoblastoma
    Richard Hurwitz; Fiscal Year: 2003
    ..Numerous preclinical and clinical trials have shown that adenoviral vectors used to deliver the Herpes thymidine kinase gene followed by ganciclovir can be safely administered...
  15. ZIDOVUDINE METABOLISM: REBRETRON TREATED HUMAN PATIENTS
    Jose Rodriguez; Fiscal Year: 2003
    ..in vivo with ZDV, diminishing the formation of ZDV intracellular metabolites (ZDV-MP and ZDV-TP), by increasing thymidine triphosphate (dTTP) intracellular concentrations which inhibits thymidine kinase 1...
  16. DLI using genetically modified T cells: Phase 1 trial
    John Dipersio; Fiscal Year: 2005
    ..The central hypotheses of this study are the following: Retroviral mediated transfer of a chimeric CD34-Thymidine Kinase (CD34-TK75) suicide gene into human T cells to control GvHD can be performed safely, and will result in ..
  17. Heart mitochondrial toxicity of antiviral nucleosides
    Edward McKee; Fiscal Year: 2006
    ..in both isolated heart mitochondria and in the isolated perfused rat heart under conditions in which labeled thymidine is readily converted to labeled TTP in both models...
  18. IMPROVING SUICIDE GENES FOR CANCER GENE THERAPY
    Margaret Black; Fiscal Year: 2004
    ..Current protocols to elicit tumor reduction utilize Herpes Simplex Virus type 1(HSV) thymidine kinase (TK) with the prodrug ganciclovir (GCV) or the E...