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| codonSummarySummary: A set of three nucleotides in a protein coding sequence that specifies individual amino acids or a termination signal (CODON, TERMINATOR). Most codons are universal, but some organisms do not produce the transfer RNAs (RNA, TRANSFER) complementary to all codons. These codons are referred to as unassigned codons (CODONS, NONSENSE). Top Publications
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Publications
Synonymous but not the same: the causes and consequences of codon biasJoshua B Plotkin
Department of Biology and Program in Applied Mathematics and Computational Science, University of Pennsylvania, 433 South University Avenue, Philadelphia, Pennsylvania 19104, USA
Nat Rev Genet 12:32-42. 2011..As a result, an understanding of codon bias is central to fields as diverse as molecular evolution and biotechnology...
Coding-sequence determinants of gene expression in Escherichia coliGrzegorz Kudla
Department of Biology and Program in Applied Mathematics and Computational Science, University of Pennsylvania, Philadelphia, PA 19104, USA
Science 324:255-8. 2009..GFP messenger RNA (mRNA) levels, mRNA degradation patterns, and bacterial growth rates also varied, but codon bias did not correlate with gene expression...
Genome-wide analysis in vivo of translation with nucleotide resolution using ribosome profilingNicholas T Ingolia
Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, and California Institute for Quantitative Biosciences, San Francisco, CA 94158, USA
Science 324:218-23. 2009..Ribosome profiling is readily adaptable to other organisms, making high-precision investigation of protein translation experimentally accessible...
Evaluation of an improved branch-site likelihood method for detecting positive selection at the molecular levelJianzhi Zhang
Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, USA
Mol Biol Evol 22:2472-9. 2005..Recently, Yang, Z., and R. Nielsen (2002. Codon-substitution models for detecting molecular adaptation at individual sites along specific lineages. Mol. Biol...
An evolutionarily conserved mechanism for controlling the efficiency of protein translationTamir Tuller
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Cell 141:344-54. 2010..We suggest that the slow "ramp" at the beginning of mRNAs serves as a late stage of translation initiation, forming an optimal and robust means to reduce ribosomal traffic jams, thus minimizing the cost of protein expression...
Translation efficiency is determined by both codon bias and folding energyTamir Tuller
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Proc Natl Acad Sci U S A 107:3645-50. 2010..mechanisms by which this effect is achieved are controversial; although some previous studies have suggested that codon bias is the most important determinant of translation efficiency, a recent study suggested that mRNA folding at ..
Selection on codon biasRuth Hershberg
Department of Biological Sciences, Stanford University, Stanford, California 94305, USA
Annu Rev Genet 42:287-99. 2008In a wide variety of organisms, synonymous codons are used with different frequencies, a phenomenon known as codon bias...
Differential arginylation of actin isoforms is regulated by coding sequence-dependent degradationFangliang Zhang
Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
Science 329:1534-7. 2010..This degradation mechanism, coupled to nucleotide coding sequence, may regulate protein arginylation in vivo...
Choosing appropriate substitution models for the phylogenetic analysis of protein-coding sequencesBeth Shapiro
Mol Biol Evol 23:7-9. 2006..This problem is exacerbated by the exclusion of codon-based models from commonly employed model selection techniques, presumably due to the computational cost ..
Design parameters to control synthetic gene expression in Escherichia coliMark Welch
DNA2 0, Menlo Park, CA, USA
PLoS ONE 4:e7002. 2009..Protein-coding sequences are commonly re-designed to enhance expression, but there are no experimentally supported design principles...
Datamonkey: rapid detection of selective pressure on individual sites of codon alignmentsSergei L Kosakovsky Pond
Antiviral Research Center, University of California, San Diego, CA 92103, USA
Bioinformatics 21:2531-3. 2005..AVAILABILITY: http://www.datamonkey.org. In the future, we plan to expand the collection of available analytic tools, and provide a package for installation on other systems...
PAL2NAL: robust conversion of protein sequence alignments into the corresponding codon alignmentsMikita Suyama
European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany
Nucleic Acids Res 34:W609-12. 2006PAL2NAL is a web server that constructs a multiple codon alignment from the corresponding aligned protein sequences...
Real-time tRNA transit on single translating ribosomes at codon resolutionSotaro Uemura
Department of Structural Biology, Stanford University School of Medicine, Stanford, California 94305 5126, USA
Nature 464:1012-7. 2010..Translation at each codon is monitored by stable binding of transfer RNAs (tRNAs)-labelled with distinct fluorophores-to translating ..
A role for codon order in translation dynamicsGina Cannarozzi
Institute of Computational Science, ETH Zurich, 8092 Zurich, Switzerland
Cell 141:355-67. 2010..Here, we show that in coding sequences, once a particular codon has been used, subsequent occurrences of the same amino acid do not use codons randomly, but favor codons that use ..
Predicting the evolution of human influenza AR M Bush
Department of Ecology and Evolutionary Biology, University of California, Irvine, CA 92697, USA
Science 286:1921-5. 1999..However, not all codons in these sites had predictive value. Monitoring new H3 isolates for additional changes in positively selected codons might help identify the most fit extant viral strains that arise during antigenic drift...
Not so different after all: a comparison of methods for detecting amino acid sites under selectionSergei L Kosakovsky Pond
Antiviral Research Center, University of California San Diego, USA
Mol Biol Evol 22:1208-22. 2005..We demonstrate our methods on sequence data from the human immunodeficiency virus type 1 env and pol genes and simulated alignments...
Codon usage bias from tRNA's point of view: redundancy, specialization, and efficient decoding for translation optimizationEduardo P C Rocha
Unité Génétique des Génomes Bactériens, Institut Pasteur, 75724 Paris Cedex 15, France
Genome Res 14:2279-86. 2004The selection-mutation-drift theory of codon usage plays a major role in the theory of molecular evolution by explaining the co-evolution of codon usage bias and tRNA content in the framework of translation optimization...
A "silent" polymorphism in the MDR1 gene changes substrate specificityChava Kimchi-Sarfaty
Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA
Science 315:525-8. 2007..We hypothesize that the presence of a rare codon, marked by the synonymous polymorphism, affects the timing of cotranslational folding and insertion of P-gp into ..
Score-based prediction of genomic islands in prokaryotic genomes using hidden Markov modelsStephan Waack
, , Lotzestr, 16-18, , Germany
BMC Bioinformatics 7:142. 2006..It is based on the analysis of codon usage (CU) of each individual gene of a genome under study...
How the sequence of a gene can tune its translationKurt Fredrick
Department of Microbiology, Ohio State Biochemistry Program, and Center for RNA Biology, The Ohio State University, Columbus, OH 43210, USA
Cell 141:227-9. 2010..Two new studies (Cannarrozzi et al., 2010; Tuller et al., 2010) now foster the idea that patterns of codon usage can control ribosome speed, fine-tuning translation to increase the efficiency of protein synthesis.
Germ-line mutations of the RET proto-oncogene in multiple endocrine neoplasia type 2AL M Mulligan
Department of Pathology, University of Cambridge, UK
Nature 363:458-60. 1993..Further, 19 of these 20 mutations affect the same conserved cysteine residue at the boundary of the RET extracellular and transmembrane domains...
Codon usage in twelve species of DrosophilaSaverio Vicario
Department of Ecology and Evolutionary Biology, Yale University, New Haven, CT 06520 8105, USA
BMC Evol Biol 7:226. 2007b>Codon usage bias (CUB), the uneven use of synonymous codons, is a ubiquitous observation in virtually all organisms examined...
Effective population size and the efficacy of selection on the X chromosomes of two closely related Drosophila speciesPeter Andolfatto
Department of Ecology and Evolutionary Biology and Lewis Sigler Institute for Integrative Genomics, Princeton University, USA
Genome Biol Evol 3:114-28. 2011..We observe significantly higher levels of polymorphism and evidence for stronger selection on codon usage bias in D...
Natural selection on synonymous and nonsynonymous mutations shapes patterns of polymorphism in Populus tremulaPar K Ingvarsson
Umea Plant Science Centre, Department of Ecology and Environmental Science, Umea University, Umea, Sweden
Mol Biol Evol 27:650-60. 2010..tremula. The results presented here show that this has resulted in an increase in codon bias in P. tremula, consistent with stronger selection acting on synonymous codon usage...
Impact of translational error-induced and error-free misfolding on the rate of protein evolutionJian Rong Yang
Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat Sen University, Guangzhou, PR China
Mol Syst Biol 6:421. 2010..These findings provide unambiguous support to the role of protein-misfolding-avoidance in determining the rate of protein sequence evolution...
Frequent somatic mutations of GNAQ in uveal melanoma and blue naeviCatherine D Van Raamsdonk
Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia V6T1Z3, Canada
Nature 457:599-602. 2009..The mutations occur exclusively in codon 209 in the Ras-like domain and result in constitutive activation, turning GNAQ into a dominant acting oncogene...
Isocitrate dehydrogenase 1 codon 132 mutation is an important prognostic biomarker in gliomasMarc Sanson
L Institut National de la Santé et de la Recherche Médicale U711, Fédération de Neurologie Mazarin, Groupe Hospitalier Pitie Salpetriere, 75651, Paris Cedex 13, France
J Clin Oncol 27:4150-4. 2009Unexpected mutations affecting the isocitrate dehydrogenase (IDH1) gene at codon 132 have been found in 12% of glioblastomas.
General rules for optimal codon choiceRuth Hershberg
Department of Biology, Stanford University, Stanford, California, United States of America
PLoS Genet 5:e1000556. 2009..Once the effect of the genomic GC content on selectively favored codon choice is taken into account, additional universal amino acid specific rules governing the identity of favored ..
Estimating translational selection in eukaryotic genomesMario dos Reis
School of Crystallography, Birkbeck College, London, UK
Mol Biol Evol 26:451-61. 2009Natural selection on codon usage is a pervasive force that acts on a large variety of prokaryotic and eukaryotic genomes...
The aflatoxin-induced TP53 mutation at codon 249 (R249S): biomarker of exposure, early detection and target for therapyDoriane Gouas
International Agency for Research on Cancer, 150 cours Albert Thomas, 69372 Lyon Cedex 08, France
Cancer Lett 286:29-37. 2009..HCC) in high-incidence areas (sub-Saharan Africa, South-Eastern Asia) often contains a somatic mutation at codon 249 in TP53 (R249S). This mutation is rare in low-incidence areas of Europe and the United States...
Assays to detect beta-tubulin codon 200 polymorphism in Trichuris trichiura and Ascaris lumbricoidesAissatou Diawara
Institute of Parasitology, McGill University, Quebec, Canada
PLoS Negl Trop Dis 3:e397. 2009..We have developed pyrosequencer assays for codon 200 (TTC or TAC) in A. lumbricoides and T. trichiura to screen for this single nucleotide polymorphism (SNP).
Investigating protein-coding sequence evolution with probabilistic codon substitution modelsMaria Anisimova
Institute of Computational Science, Swiss Federal Institute of Technology, Zurich, Switzerland
Mol Biol Evol 26:255-71. 2009..by the true explosion in the number of recent studies both developing and ameliorating probabilistic models of codon evolution...
An empirical codon model for protein sequence evolutionCarolin Kosiol
EMBL European corrected Bioinformatics Institute, Hinxton, United Kingdom
Mol Biol Evol 24:1464-79. 2007In the past, 2 kinds of Markov models have been considered to describe protein sequence evolution. Codon-level models have been mechanistic with a small number of parameters designed to take into account features, such as transition-..
Recombination and base composition: the case of the highly self-fertilizing plant Arabidopsis thalianaG Marais
Institute of Cell, Animal and Population Biology, University of Edinburgh, EH9 3JT Edinburgh, UK
Genome Biol 5:R45. 2004..thaliana genome. We show that, in this species, both codon-usage bias and GC content do not correlate with the local rates of crossing over, in agreement with our ..
Determinants of synonymous and nonsynonymous variability in three species of DrosophilaPenelope R Haddrill
Institute of Evolutionary Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, United Kingdom
Mol Biol Evol 28:1731-43. 2011..We found evidence for stronger selection at X-linked loci, consistent with their higher levels of codon usage bias...
Extreme reconfiguration of plastid genomes in the angiosperm family Geraniaceae: rearrangements, repeats, and codon usageMary M Guisinger
Section of Integrative Biology, University of Texas, Austin, USA
Mol Biol Evol 28:583-600. 2011..Geraniaceae plastomes were found to be highly variable in size, gene content and order, repetitive DNA, and codon usage...
Determinants of translation efficiency and accuracyHila Gingold
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel
Mol Syst Biol 7:481. 2011..A practical demonstration of a better understanding of the process would be a more accurate prediction of the proteome, given the transcriptome at a diversity of biological conditions...
Translation efficiency in humans: tissue specificity, global optimization and differences between developmental stagesYedael Y Waldman
Blavatnik School of Computer Science, Tel Aviv University, Ramat Aviv 69978, Israel
Nucleic Acids Res 38:2964-74. 2010Various studies in unicellular and multicellular organisms have shown that codon bias plays a significant role in translation efficiency (TE) by co-adaptation to the tRNA pool...
The codon 620 tryptophan allele of the lymphoid tyrosine phosphatase (LYP) gene is a major determinant of Graves' diseaseM R Velaga
Institute of Human Genetics, University of Newcastle upon Tyne NE1 3BZ, United Kingdom
J Clin Endocrinol Metab 89:5862-5. 2004..a single nucleotide polymorphism (SNP), encoding a functional arginine to tryptophan residue change at LYP codon 620 has been shown to be associated with type 1 diabetes and other autoimmune disorders...
Viral adaptation to host: a proteome-based analysis of codon usage and amino acid preferencesIris Bahir
Department of Biological Chemistry, Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel
Mol Syst Biol 5:311. 2009..We consider their respective amino acid and codon usages and compare them among the viruses and their hosts...
The complete mitochondrial genome of Diadegma semiclausum (hymenoptera: ichneumonidae) indicates extensive independent evolutionary eventsShu jun Wei
Institute of Insect Sciences, Zhejiang University, Hangzhou 310029, China
Genome 52:308-19. 2009....
GroEL dependency affects codon usage--support for a critical role of misfolding in gene evolutionTobias Warnecke
Department of Biology and Biochemistry, University of Bath, Bath, UK
Mol Syst Biol 6:340. 2010..We propose that putative error limitation in cis can be elucidated by examining the interaction between codon usage and chaperoning processes...
Heterologous protein expression is enhanced by harmonizing the codon usage frequencies of the target gene with those of the expression hostEvelina Angov
Molecular Parasitology, Division of Malaria Vaccine Development, Walter Reed Army Institute of Research, Silver Spring, Maryland, United States of America
PLoS ONE 3:e2189. 2008Synonymous codon replacement can change protein structure and function, indicating that protein structure depends on DNA sequence...
Germline dinucleotide mutation in codon 883 of the RET proto-oncogene in multiple endocrine neoplasia type 2B without codon 918 mutationO Gimm
Translational Research Laboratory, Dana Farber Cancer Institute, Boston, Massachusetts, USA
J Clin Endocrinol Metab 82:3902-4. 1997..More than 95% of all MEN 2B cases are caused by germline mutation at codon 918 (M918T) in exon 16 of the RET proto-oncogene...
Instruction of translating ribosome by nascent peptideFeng Gong
Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
Science 297:1864-7. 2002..Replacing the tnaC stop codon with a tryptophan codon allows tryptophan-charged tryptophan transfer RNA to substitute for tryptophan as inducer...
Mutation-selection models of codon substitution and their use to estimate selective strengths on codon usageZiheng Yang
Department of Biology, Galton Laboratory, University College London, London, United Kingdom
Mol Biol Evol 25:568-79. 2008Current models of codon substitution are formulated at the levels of nucleotide substitution and do not explicitly consider the separate effects of mutation and selection...
Patterns of codon usage bias in Silene latifoliaSuo Qiu
State Key Laboratory of Biocontrol and Key Laboratory of Gene Engineering of the Ministry of Education, Sun Yat Sen University, Guangzhou 510275, China
Mol Biol Evol 28:771-80. 2011Patterns of codon usage bias (CUB) convey useful information about the selection on synonymous codons induced by gene expression and contribute to an understanding of substitution patterns observed at synonymous sites...
Generation and immunogenicity of novel HIV/AIDS vaccine candidates targeting HIV-1 Env/Gag-Pol-Nef antigens of clade CCarmen Elena Gómez
Department of Molecular and Cellular Biology, Centro Nacional de Biotecnologia, CSIC, Ciudad Universitaria Cantoblanco, 28049 Madrid, Spain
Vaccine 25:1969-92. 2007..in transgenic HHD mice the immunogenicity of two attenuated poxvirus vectors expressing in a single locus (TK) the codon optimized HIV-1 genes encoding gp120 and Gag-Pol-Nef (GPN) polyprotein of clade C (referred as MVA-C and NYVAC-C)...
Mutations at embB codon 306 are an important molecular indicator of ethambutol resistance in Mycobacterium tuberculosisAngela M Starks
Division of Tuberculosis Elimination, National Center for HIV AIDS, Viral Hepatitis, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
Antimicrob Agents Chemother 53:1061-6. 2009..However, recent reports have described the presence of embB mutations, especially those at embB codon 306, in isolates susceptible to ethambutol...
Codon usage bias and the evolution of influenza A viruses. Codon Usage Biases of Influenza VirusEmily H M Wong
Department of Microbiology, The University of Hong Kong, Pokfulam, Hong Kong, China
BMC Evol Biol 10:253. 2010..As the replication of the influenza virus is based on its host's machinery, codon usage of its viral genes might be subject to host selection pressures, especially after interspecies transmission...
Biased gene conversion affects patterns of codon usage and amino acid usage in the Saccharomyces sensu stricto group of yeastsRichard J Harrison
Institute of Evolutionary Biology, School of Biological Sciences, Kings Buildings, West Mains Road, Edinburgh EH9 3JT, United Kingdom
Mol Biol Evol 28:117-29. 2011Patterns of synonymous codon usage vary between organisms and are controlled by neutral processes (such as drift and mutation) as well as by selection...
Gene disruption in Candida albicans using a synthetic, codon-optimised Cre-loxP systemPaul M J Dennison
Aberdeen Fungal Group, School of Medical Sciences, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen AB25 2ZD, UK
Fungal Genet Biol 42:737-48. 2005..These markers were then resolved from the C. albicans genome using a synthetic codon-optimised cre recombinase gene, with near 100% efficiency...
Forces that influence the evolution of codon biasPaul M Sharp
Institute of Evolutionary Biology, University of Edinburgh, Kings Buildings, Edinburgh EH9 3JT, UK
Philos Trans R Soc Lond B Biol Sci 365:1203-12. 2010..The strength of selection on codon usage can be estimated by two different approaches...
Estimating selection intensity on synonymous codon usage in a nonequilibrium populationKai Zeng
Institute of Evolutionary Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, UK
Genetics 183:651-62, 1SI-23SI. 2009b>Codon usage bias is the nonrandom use of synonymous codons for the same amino acid. Most population genetic models of codon usage evolution assume that the population is at mutation-selection-drift equilibrium...
Evolutionary basis of codon usage and nucleotide composition bias in vertebrate DNA virusesLaura A Shackelton
Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, UK
J Mol Evol 62:551-63. 2006Understanding the extent and causes of biases in codon usage and nucleotide composition is essential to the study of viral evolution, particularly the interplay between viruses and host cells or immune responses...
Patterns of molecular evolution in Caenorhabditis preclude ancient origins of selfingAsher D Cutter
Department of Ecology and Evolutionary Biology and the Centre for the Analysis of Genome Evolution and Function, University of Toronto, Toronto, Ontario M5S 3G5, Canada
Genetics 178:2093-104. 2008..Here we contrast rates of nucleotide substitution and codon usage bias among thousands of orthologous groups of genes in six species of Caenorhabditis, including the classic ..
Trm9-catalyzed tRNA modifications link translation to the DNA damage responseUlrike Begley
Department of Biomedical Sciences, GenNYsis Center for Excellence in Cancer Genomics, University at Albany, State University of New York, Rensselaer, NY 12144, USA
Mol Cell 28:860-70. 2007..Additionally, we identified 425 genes, which included YEF3, RNR1, and RNR3, with a unique codon usage pattern linked to Trm9...
The mitochondrial genome of the smaller tea tortrix Adoxophyes honmai (Lepidoptera: Tortricidae)Eun Seung Lee
Department of Plant Medicine, Chungbuk National University, Cheongju, Republic of Korea
Gene 373:52-7. 2006..Nucleotide composition, amino acid composition and codon usage are in the range of values estimated from other insect mitogenomes. In AT rich region of A...
Characterization and modeling of the Haemophilus influenzae core and supragenomes based on the complete genomic sequences of Rd and 12 clinical nontypeable strainsJustin S Hogg
Allegheny General Hospital, Allegheny Singer Research Institute, Center for Genomic Sciences, Pittsburgh, Pennsylvania 15212, USA
Genome Biol 8:R103. 2007..Studies based on random sequencing of multiple strain libraries suggested that free-living bacterial species possess a supragenome that is much larger than the genome of any single bacterium...
RET codon 634 mutations in multiple endocrine neoplasia type 2: variable clinical features and clinical outcomeMarcia K Puñales
Endocrine Division, Hospital de Clinicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre 90035 003, Rio Grande do Sul, Brazil
J Clin Endocrinol Metab 88:2644-9. 2003..Sixty-nine individuals from 12 different families presented a codon 634 mutation, the most prevailing missense mutation in our series...
Two germline missense mutations at codons 804 and 806 of the RET proto-oncogene in the same allele in a patient with multiple endocrine neoplasia type 2B without codon 918 mutationA Miyauchi
Kuma Hospital, Kobe
Jpn J Cancer Res 90:1-5. 1999..Combinations of mutations of the RET proto-oncogene may cause oncogenic activities different from those of single mutations...
Gene expression and protein length influence codon usage and rates of sequence evolution in Populus tremulaPar K Ingvarsson
Umea Plant Science Centre, Department of Ecology and Environmental Science, Umea University, Umea, Sweden
Mol Biol Evol 24:836-44. 2007b>Codon bias is generally thought to be determined by a balance between mutation, genetic drift, and natural selection on translational efficiency...
Following translation by single ribosomes one codon at a timeJin Der Wen
Department of Chemistry, University of California, Berkeley, California 94720, USA
Nature 452:598-603. 2008..Each translocation step measures three bases--one codon-and occurs in less than 0.1 s...
Transfer of embB codon 306 mutations into clinical Mycobacterium tuberculosis strains alters susceptibility to ethambutol, isoniazid, and rifampinHassan Safi
Division of Infectious Disease and Center for Emerging Pathogens, Department of Medicine, New Jersey Medical School, University of Medicine and Dentistry of New Jersey, Newark, New Jersey 07103, USA
Antimicrob Agents Chemother 52:2027-34. 2008..a major mechanism of ethambutol (EMB) resistance in clinical studies of Mycobacterium tuberculosis, mutations in codon 306 of the embB gene (embB306) have also been detected in EMB-susceptible clinical isolates...
Improved characterisation of among-lineage rate variation in cetacean mitogenomes using codon-partitioned relaxed clocksSimon Y W Ho
Research School of Biology, Centre for Macroevolution and Macroecology, Australian National University, Canberra, ACT, 0200, Australia
Mitochondrial DNA 21:138-46. 2010..However, this is rarely the case for differences in the lineage-specific patterns of rate variation among sites...
Effect of codon optimization and subcellular targeting on Toxoplasma gondii antigen SAG1 expression in tobacco leaves to use in subcutaneous and oral immunization in miceMelina Laguía-Becher
IIB INTECH, Camino de Circunvalación Km 6, Provincia de Buenos Aires, Argentina
BMC Biotechnol 10:52. 2010b>Codon optimization and subcellular targeting were studied with the aim to increase the expression levels of the SAG178-322 antigen of Toxoplasma gondii in tobacco leaves...
Frequency of embB codon 306 mutations in ethambutol-susceptible and -resistant clinical Mycobacterium tuberculosis isolates in KuwaitS Ahmad
Department of Microbiology, Kuwait University, P O Box 24923, Safat 13110, Kuwait
Tuberculosis (Edinb) 87:123-9. 2007..Recent reports of embB306 mutations in ethambutol-resistant and ethambutol-susceptible drug-resistant strains of Mycobacterium tuberculosis have questioned the significance of these mutations in conferring resistance to ethambutol (EMB)...
Evaluation of the effect of CpG hypermutability on human codon substitutionKazuharu Misawa
Chiba Industry Advancement Center, 2 6 Nakase, Mihama ku, Chiba 261 7126, Japan
Gene 431:18-22. 2009..In this study, we evaluate the effect of CpG hypermutability on codon substitution by comparing thousands of coding regions in the human and chimpanzee genomes and by inferring ..
Positive selection at sites of multiple amino acid replacements since rat-mouse divergenceGeorgii A Bazykin
Department of Ecology and Evolutionary Biology, Princeton University, Princeton, New Jersey 08544, USA
Nature 429:558-62. 2004..This pattern cannot be explained by correlated mutation or episodes of relaxed negative selection, but instead indicates that positive selection acts at many sites of rapid, successive amino acid replacement...
Clustering of codons with rare cognate tRNAs in human genes suggests an extra level of expression regulationJoanna L Parmley
Centre for Molecular and Biomolecular Informatics, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
PLoS Genet 5:e1000548. 2009..Rather than averaging the codon usage of complete genes, we scan the genes for windows with deviating codon usage...
The influence of anticodon-codon interactions and modified bases on codon usage bias in bacteriaWenqi Ran
Department of Physics and Astronomy, McMaster University, Hamilton, Ontario, Canada
Mol Biol Evol 27:2129-40. 2010Most transfer RNAs (tRNAs) can translate more than one synonymous codon, and most codons can be translated by more than one isoacceptor tRNA...
The extent of codon usage bias in human RNA viruses and its evolutionary originGareth M Jenkins
Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, UK
Virus Res 92:1-7. 2003Revealing the determinants of codon usage bias is central to the understanding of factors governing viral evolution...
The relationship between specific RET proto-oncogene mutations and disease phenotype in multiple endocrine neoplasia type 2. International RET mutation consortium analysisC Eng
Division of Cancer Epidemiology and Control, Dana Farber Cancer Institute, Department of Medicine, Harvard Medical School, Boston, MA 02115 6084, USA
JAMA 276:1575-9. 1996..The purpose of this study was to establish the relationship between specific mutations and the presence of certain disease features in MEN 2 which could help in clinical decision making...
Background selection in single genes may explain patterns of codon biasLaurence Loewe
Institute of Evolutionary Biology, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3JT, United Kingdom
Genetics 175:1381-93. 2007..the effective population sizes of different regions of the same gene, consistent with observed differences in codon usage bias along genes. It may also help to cause the observed effects of gene length and introns on codon usage...
Patterns of p53 G-->T transversions in lung cancers reflect the primary mutagenic signature of DNA-damage by tobacco smokeP Hainaut
International Agency for Research on Cancer WHO, 150 cours Albert Thomas, 69372 Lyon Cedex, France
Carcinogenesis 22:367-74. 2001..Our data reinforce the notion that p53 mutations in lung cancers can be attributed to direct DNA damage from cigarette smoke carcinogens rather than to selection of pre-existing endogenous mutations...
Mutation pressure shapes codon usage in the GC-Rich genome of foot-and-mouth disease virusJincheng Zhong
University of Electronic Science and Technology of China, Chengdu, Sichuan 610054, PR China
Virus Genes 35:767-76. 2007..In this study, we report the results of a survey of codon usage bias of FMD virus (FMDV) representing all seven serotypes (A, O, C, Asia 1, SAT 1, SAT 2, and SAT 3)...
CAIcal: a combined set of tools to assess codon usage adaptationPere Puigbò
Department of Biochemistry and Biotechnology, Rovira i Virgili University URV, Campus Sescelades, c Marcelli Domingo s n, 43007 Tarragona, Spain
Biol Direct 3:38. 2008The Codon Adaptation Index (CAI) was first developed to measure the synonymous codon usage bias for a DNA or RNA sequence...
Measuring and detecting molecular adaptation in codon usage against nonsense errors during protein translationMichael A Gilchrist
Department of Ecology and Evolutionary Biology, University of Tennessee, Knoxville, Tennessee 37996 1610, USA
Genetics 183:1493-505. 2009b>Codon usage bias (CUB) has been documented across a wide range of taxa and is the subject of numerous studies...
Increased incidence of rare codon clusters at 5' and 3' gene termini: implications for functionThomas F Clarke
Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, USA
BMC Genomics 11:118. 2010..The process of translation can be affected by the use of rare versus common codons within the mRNA transcript...
Gene optimization mechanisms: a multi-gene study reveals a high success rate of full-length human proteins expressed in Escherichia coliBarbara Maertens
QIAGEN GmbH, Qiagen Strasse 1, Hilden 40724, Germany
Protein Sci 19:1312-26. 2010..code is universal, but recombinant protein expression in heterologous systems is often hampered by divergent codon usage...
On relevance of codon usage to expression of synthetic and natural genes in Escherichia coliFran Supek
Division of Electronics, Rudjer Boskovic Institute, 10000 Zagreb, Croatia
Genetics 185:1129-34. 2010A recent investigation concluded that codon bias did not affect expression of green fluorescent protein (GFP) variants in Escherichia coli, while stability of an mRNA secondary structure near the 5' end played a dominant role...
Codon usage bias and recombination events for neuraminidase and hemagglutinin genes in Chinese isolates of influenza A virus subtype H9N2Xudong Liu
State Key Laboratory of Structural Analysis for Industrial Equipment, Faculty of Vehicle Engineering and Mechanics, Dalian University of Technology, Dalian, 116023, Liaoning, China
Arch Virol 155:685-93. 2010..and hemagglutinin sequences (HA) of 128 Chinese isolates of influenza A virus subtype H9N2 were analyzed to reveal codon usage bias and recombination events...
Mutation-selection models of coding sequence evolution with site-heterogeneous amino acid fitness profilesNicolas Rodrigue
Department of Biology, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada
Proc Natl Acad Sci U S A 107:4629-34. 2010..To this end, codon-based evolutionary models have been proposed as pertinent means of studying long-range evolutionary patterns and ..
A redshifted codon-optimized firefly luciferase is a sensitive reporter for bioluminescence imagingHenrike Caysa
Department of Medicine IV, Martin Luther University Halle Wittenberg, Halle, Germany
Photochem Photobiol Sci 8:52-6. 2009..efficiency of light through tissue increases greatly for wavelengths above 600 nm we examined whether a redshifted codon-optimized firefly luciferase (lambdamax=615 nm) could be successfully employed as a sensitive reporter in ..
Genes optimized by evolution for accurate and fast translation encode in Archaea and Bacteria a broad and characteristic spectrum of protein functionsConrad von Mandach
University of Hagen, Germany
BMC Genomics 11:617. 2010..This codon usage bias (CUB) improves translational accuracy and speed and is one of several factors optimizing cell growth...
Solving the riddle of codon usage preferences: a test for translational selectionMario dos Reis
School of Crystallography, Birkbeck College, University of London, Malet Street, London WC1E 7HX, UK
Nucleic Acids Res 32:5036-44. 2004..Accordingly, genome size also presents upper and lower boundaries beyond which selection on codon usage is not possible...
Translational efficiency of a non-AUG initiation codon is significantly affected by its sequence context in yeastShun Jia Chen
Department of Life Science, National Central University, Jung li, Taiwan 32001
J Biol Chem 283:3173-80. 2008..of mrna for yeast glycyl-tRNA synthetase is alternatively initiated from UUG and a downstream AUG initiation codon. Evidence presented here shows that unlike an AUG initiation codon, efficiency of this non-AUG initiation codon is ..
Fully codon-optimized luciferase uncovers novel temperature characteristics of the Neurospora clockVan D Gooch
Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA
Eukaryot Cell 7:28-37. 2008We report the complete reconstruction of the firefly luciferase gene, fully codon optimized for expression in Neurospora crassa...
The evolution of sex-biased genes and sex-biased gene expressionHans Ellegren
Department of Evolutionary Biology, Uppsala University, Norbyvagen 18D, SE 752 36 Uppsala, Sweden
Nat Rev Genet 8:689-98. 2007..Sex-biased gene expression has implications beyond just evolutionary biology, including for medical genetics...
Relaxation of yeast mitochondrial functions after whole-genome duplicationHuifeng Jiang
Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853, USA
Genome Res 18:1466-71. 2008..Furthermore, codon usage bias was relaxed for these genes in post-WGD yeast species...
Conflicting selection pressures on synonymous codon use in yeast suggest selection on mRNA secondary structuresNina Stoletzki
Ludwig Maximilan Universität, Biocenter, Grosshadernerstr, 2, D 82151 Planegg Martinsried, Germany
BMC Evol Biol 8:224. 2008....
Minority human immunodeficiency virus type 1 variants in antiretroviral-naive persons with reverse transcriptase codon 215 revertant mutationsYumi Mitsuya
Department of Medicine, Division of Infectious Diseases, Stanford University Medical Center, Stanford, California 94305, USA
J Virol 82:10747-55. 2008....
A general model of codon bias due to GC mutational biasGareth A Palidwor
Ottawa Hospital Research Institute, Ottawa, Canada
PLoS ONE 5:e13431. 2010In spite of extensive research on the effect of mutation and selection on codon usage, a general model of codon usage bias due to mutational bias has been lacking...
A rare polymorphism of the COX7B2 gene in a Cantonese family with nasopharyngeal carcinomaHui Liang
Cancer Center, Sun Yat Sen University, Guangzhou 510060, China
Sci China C Life Sci 47:449-53. 2004..The change 78T > A at codon 26 which leads to CAT26CAA (His26Gln) was shared by patients from family 31 that carried the susceptibility ..
[The molecular mechanism of evolution of changes in the genetic code]Ana C Gomes
Mol Biol (Mosk) 40:634-9. 2006..In C. albicans, the leucine CUG codon is decoded as serine through structural alterations of the translational machinery, in particular, of a Ser-..
A unified model of codon reassignment in alternative genetic codesSupratim Sengupta
Department of Physics and Astronomy, McMaster University, Hamilton, Ontario, Canada
Genetics 170:831-40. 2005..We present a new framework for codon reassignment that incorporates two previously proposed mechanisms (codon disappearance and ambiguous intermediate) ..
Evidence for abundant slightly deleterious polymorphisms in bacterial populationsAustin L Hughes
Department of Biological Sciences, University of South Carolina, Columbia, South Carolina 29208, USA
Genetics 169:533-8. 2005..The major exceptions to this trend were seen among surface proteins, particularly those of bacteria parasitic on vertebrates, which included a number of cases of polymorphisms apparently maintained by balancing selection...
Mutation and selection on the anticodon of tRNA genes in vertebrate mitochondrial genomesXuhua Xia
Department of Biology, University of Ottawa, 150 Louis, P O Box 450, Station A, Ottawa, Ontario, Canada K1N 6N5
Gene 345:13-20. 2005..For the 12 coding sequences (CDS) collinear with the L-strand, NNY synonymous codon families (where N stands for any of the four nucleotides and Y stands for either C or U) end mostly with C, and ..
Domains, amino acid residues, and new isoforms of Caenorhabditis elegans diacylglycerol kinase 1 (DGK-1) important for terminating diacylglycerol signaling in vivoAntony M Jose
Department of Molecular, Cellular, and Developmental Biology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
J Biol Chem 280:2730-6. 2005..expected to inactivate the kinase domain retained very little physiological function, but we found two stop codon mutants predicted to truncate DGK-1 before its kinase domain that retained significantly more function...
Overlapping messages and survivabilityOfer Peleg
Genome Diversity Center, Institute of Evolution, Haifa University Mt Carmel, Haifa 31905, Israel
J Mol Evol 59:520-7. 2004..Over a broad range of conditions the reduction of the target size prevails, thus making the overlapping of messages advantageous...
Empirical codon substitution matrixAdrian Schneider
Institute of Computational Science, Swiss Federal Institute of Technology, Zurich, Switzerland
BMC Bioinformatics 6:134. 2005b>Codon substitution probabilities are used in many types of molecular evolution studies such as determining Ka/Ks ratios, creating ancestral DNA sequences or aligning coding DNA...
Research Grants
- Role of troponin T isoforms in nemaline myopathyJian Ping Jin; Fiscal Year: 2010..In an alternative approach, suppression of the ANM nonsense stop codon will also be explored using emerging reagents...
- Role of troponin T isoforms in nemaline myopathyJian Ping Jin; Fiscal Year: 2009..In an alternative approach, suppression of the ANM nonsense stop codon will also be explored using emerging reagents...
- BIOCHEMISTRY OF PRE-MRNA SPLICINGADRIAN KRAINER; Fiscal Year: 2009..NMD degrades mature mRNAs with a premature termination codon (PTC)...
- BIOCHEMISTRY OF PRE-MRNA SPLICINGADRIAN KRAINER; Fiscal Year: 2007..NMD degrades mature mRNAs with a premature termination codon (PTC)...
- A SARS-CoV Spike Protein VaccineB Sim; Fiscal Year: 2005..Our experience with expression of proteins in Pichia pastoris indicates that altering codon usage based on our proprietary information leads to enhanced expression of recombinant proteins and in vivo ..
- Resolution of Epithelial Cell HyperplasiaYohannes Tesfaigzi; Fiscal Year: 2010..A polymorphism in the p53 gene at codon 72 (Arg/Pro) differentially affects Bcl-2 expression;p53Pro increases more efficiently than p53Arg, the pro-..
- Resolution of Epithelial Cell HyperplasiaYohannes Tesfaigzi; Fiscal Year: 2009..A polymorphism in the p53 gene at codon 72 (Arg/Pro) differentially affects Bcl-2 expression; p53Pro increases more efficiently than p53Arg, the pro-..
- APOLIPOPROTEIN B48 AND LIPOPROTEIN METABOLISMLawrence Chan; Fiscal Year: 2004..ApoB mRNA editing is a process whereby the cytidine (C) in a CAA codon in apoB-100 mRNA, encoding Gln-2153, is deaminated to a uridine (U), producing an in-frame UAA stop codon in apoB-..
- Ribosomal scanning and initiation codon selectionTatyana V Pestova; Fiscal Year: 2010..It occurs in two stages: formation of a 48S initiation complex at the initiation codon of mRNA and its joining with a 60S ribosomal subunit...
- STRUCTURE OF VIRAL RNA IN RIBOSOMAL FRAMESHIFTINGAlexander Rich; Fiscal Year: 2004..The - 1 shift in reading frames causes stop codon readthrough, and results in production of a single fusion protein...
- RNA Modifications as Biomarkers of Environmental Stress and InflammationThomas J Begley; Fiscal Year: 2010..have recently demonstrated that enzyme-catalyzed methylation of a modified uridine (mcm5U) in tRNA plays a role in codon- specific translational control after DNA damage, and we have preliminary data demonstrating that the levels of ..
- FUNCTIONAL CHARACTERIZATION OF AGT GENETIC POLYMORPHISMJun yan Hong; Fiscal Year: 2001..One missense alteration occurs at the codon 143 (isoleucine to valine)...
- Ribosomal scanning and initiation codon selectionTatyana V Pestova; Fiscal Year: 2011..It occurs in two stages: formation of a 48S initiation complex at the initiation codon of mRNA and its joining with a 60S ribosomal subunit...
- CONTROL OF ARG-2 GENE EXPRESSION IN NEUROSPORAMATTHEW SACHS; Fiscal Year: 2007..These data support a regulatory model in which ribosome stalling at the uORF termination codon in response to Arg destabilizes CPA1 mRNA by increasing the extent of nonsense codon recognition by NMD...
- CONTROL OF ARG-2 GENE EXPRESSION IN NEUROSPORAMATTHEW SACHS; Fiscal Year: 2006..This data supports a regulatory model in which ribosome stalling at the uORF termination codon in response to Arg destabilizes CPA1 mRNA by increasing the extent of nonsense codon recognition by NMD...
- CONTROL OF ARG-2 GENE EXPRESSION IN NEUROSPORAMATTHEW SACHS; Fiscal Year: 2007..These data support a regulatory model in which ribosome stalling at the uORF termination codon in response to Arg destabilizes CPA1 mRNA by increasing the extent of nonsense codon recognition by NMD...
- CONTROL OF ARG-2 GENE EXPRESSION IN NEUROSPORAMATTHEW SACHS; Fiscal Year: 2009..These data support a regulatory model in which ribosome stalling at the uORF termination codon in response to Arg destabilizes CPA1 mRNA by increasing the extent of nonsense codon recognition by NMD...
- The Biological and Chemical Function of Selenium in EnzymesROBERT J contact HONDAL; Fiscal Year: 2010..of selenocysteine (Sec) into a protein is much more complicated than the other 20 amino acids because a UGA stop codon must be recoded as a sense codon for Sec and this process requires complex cellular machinery...
- Listeria Immunotherapy for Pancreatic and Ovarian CancerThomas Dubensky; Fiscal Year: 2006..is to continue development of our Listeria monocytogenes (Lm)-based immunotherapy to target Mesothelin and codon 12-mutated activated K-ras antigens in patients with pancreatic and ovarian cancers...
- Translational Research in the DystrophinopathiesKevin Flanigan; Fiscal Year: 2002..The remainder consist of point mutations (primarily premature stop codon mutations), small deletions resulting in shift of the reading frame, and (in less than 5%) duplications...
- ENTEROHEPATIC LIPID FLUX AND APOPROTEIN BIOSYNTHESIS.NICHOLAS DAVIDSON; Fiscal Year: 2007..RNA modification that results in C to U deaminaton of a single cytidine base and creates a translational stop codon. This process occurs in the mammalian small intestine and is mediated by a multicomponent enzyme complex ..
- Gene Expression Levels Across Diverse GenomesSamuel Karlin; Fiscal Year: 2006..The main focus of our research is to evaluate gene expression levels based on codon usage...
- BACTERIAL LIVE VECTOR-BASED VACCINE AGAINST MALARIAMyron Levine; Fiscal Year: 2004..the priming potential of Shigella live vectors harboring DNA vaccines, we propose to use DNA vaccines in which codon usage has been optimized for expression by mammalian (human) cells...
- STRUCTURE AND FUNCTION OF TRANSFER RIBONUCLEIC ACIDSUttam L RajBhandary; Fiscal Year: 2010..In eubacteria, ochre suppressor tRNAs, which read the UAA stop codon also read the amber stop codon UAG. In eukaryotes, however, ochre suppressors are specific for UAA...
- EDITING OF APOLIPOPROTEIN-B MRNADONNA DRISCOLL; Fiscal Year: 2004..The editing of apolipoprotein-B (apo-B) mRNA involves the deamination of C6666 to U which converts a glutamine codon (CAA) to an in-frame stop codon (UAA)...
- tRNA editing by deamination: Balancing affinity and specificityJuan Alfonzo; Fiscal Year: 2009..amino acids and, with the exception of methionine and tryptophan, each amino acid is encoded by more than one codon. This discrepancy between codon and amino acid numbers was first explained by Crick's wobble hypothesis, ..
- tRNA editing by deamination: Balancing affinity and specificityJuan D Alfonzo; Fiscal Year: 2010..amino acids and, with the exception of methionine and tryptophan, each amino acid is encoded by more than one codon. This discrepancy between codon and amino acid numbers was first explained by Crick's wobble hypothesis, ..
- 'RECODING: DYNAMIC REPROGRAMMING OF GENETIC READOUT'John Atkins; Fiscal Year: 2004..cassettes with different defects in the gene 60 bypassing signals: poorly functioning matched take-off and landing codon pairs, extended coding gap, extended stem loop and related studies with mutants of nascent peptide affecting frame ..
- Regulation of the Na/K Pump by RNA EditingJoshua J C Rosenthal; Fiscal Year: 2010..A central premise to our approach is that naturally occurring codon changes, caused by RNA editing, can lead us to functionally important regions of the Na/K pump...
- Translation Regulation in Giardia lambliaCHING CHUNG WANG; Fiscal Year: 2010..analysis of an internal ribosomal entry site (IRES) in the Giardia-virus transcript, we identified the AUG start codon at the center of an unstructured 31 nucleotide stretch between two stem-loops, suggesting a direct recruitment of ..
- The Role of Osteoactivin in Osteoblast Development and FunctionFayez F Safadi; Fiscal Year: 2010..in mice with the null allele for OA and in mice with a natural mutation in the OA gene caused a premature stop codon that results in the generation of a truncated OA protein...
- APOLIPOPROTEIN B48 AND LIPOPROTEIN METABOLISMLawrence Chan; Fiscal Year: 1999..The apoB-48 mRNA is the product of RNA editing, whereby the first nucleotide (C) for the codon CAA, encoding Gln-2153, in apoB-100 mRNA is converted to U producing an in-frame stop codon...
- MOLECULAR ANALYSIS OF SITE-SPECIFIC TRANSLATIONAL FRAMESPhilip Farabaugh; Fiscal Year: 1993..This sequence includes no obvious secondary structure, but does include an unusual codon, AGG. This codon is recognized by a rare tRNA encoded by a single genetic locus...
