viral dna

Summary

Summary: Deoxyribonucleic acid that makes up the genetic material of viruses.

Top Publications

  1. ncbi Human papillomavirus is a necessary cause of invasive cervical cancer worldwide
    J M Walboomers
    Department of Pathology, University Hospital Vrije Universiteit, Amsterdam, The Netherlands
    J Pathol 189:12-9. 1999
  2. ncbi Epidemiologic classification of human papillomavirus types associated with cervical cancer
    Nubia Munoz
    International Agency for Research on Cancer, Lyons, France
    N Engl J Med 348:518-27. 2003
  3. pmc The causal relation between human papillomavirus and cervical cancer
    F X Bosch
    Institut Catala d Oncologia, Servei d Epidemiologia i Registre del Cancer, Gran Via Km 2 7 s n 08907 L Hospitalet de Llobregat, 08907 Barcelona, Spain
    J Clin Pathol 55:244-65. 2002
  4. ncbi Identification of herpesvirus-like DNA sequences in AIDS-associated Kaposi's sarcoma
    Y Chang
    Department of Pathology, College of Physicians and Surgeons, Columbia University, New York, NY 10032
    Science 266:1865-9. 1994
  5. ncbi Risk of hepatocellular carcinoma across a biological gradient of serum hepatitis B virus DNA level
    Chien Jen Chen
    Graduate Institute of Epidemiology, College of Public Health, National Taiwan University, Taipei, Taiwan
    JAMA 295:65-73. 2006
  6. ncbi Worldwide prevalence and genotype distribution of cervical human papillomavirus DNA in women with normal cytology: a meta-analysis
    Silvia de Sanjose
    Servei d Epidemiologia i Registre del Cancer, Institut Catala d Oncologia, Barcelona, Spain
    Lancet Infect Dis 7:453-9. 2007
  7. pmc Presence of an inducible HIV-1 latent reservoir during highly active antiretroviral therapy
    T W Chun
    Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 94:13193-7. 1997
  8. ncbi A quantitative assay for HIV DNA integration in vivo
    S L Butler
    Infectious Disease Laboratory, The Salk Institute for Biological Studies, La Jolla, California, USA
    Nat Med 7:631-4. 2001
  9. pmc Hepatitis B virus biology
    C Seeger
    Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA
    Microbiol Mol Biol Rev 64:51-68. 2000
  10. ncbi Interaction of the bovine papillomavirus E2 protein with Brd4 tethers the viral DNA to host mitotic chromosomes
    Jianxin You
    Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
    Cell 117:349-60. 2004

Research Grants

  1. Regulation of HSV-1 Gene Expression and Replication by Nuclear Hormone Receptors
    SHAOCHUNG VICTOR HSIA; Fiscal Year: 2013
  2. Molecular Mechanisms of Parvovirus Gene Expression and Replication
    Jianming Qiu; Fiscal Year: 2013
  3. Hepatitis B virus replication and DDB1
    Betty L Slagle; Fiscal Year: 2013
  4. Regulation of EBV Latency by EBNA1
    Paul M Lieberman; Fiscal Year: 2013
  5. Nanomedicine and NeuroAIDS
    Howard E Gendelman; Fiscal Year: 2013
  6. Biophysics of Drug Interactions with Viral Episomes
    James K Bashkin; Fiscal Year: 2013
  7. E2-Cellular Complexes in HPV Chromatin Transcription
    Cheng Ming Chiang; Fiscal Year: 2013
  8. Agnoprotein in JC virus virion biogenesis and replication
    Mahmut Safak; Fiscal Year: 2012
  9. Encapsidation of the poxvirus genome
    Paula Traktman; Fiscal Year: 2012
  10. Genetic Epidemiology of Life-Threatening Influenza in Children
    Adrienne G Randolph; Fiscal Year: 2013

Detail Information

Publications369 found, 100 shown here

  1. ncbi Human papillomavirus is a necessary cause of invasive cervical cancer worldwide
    J M Walboomers
    Department of Pathology, University Hospital Vrije Universiteit, Amsterdam, The Netherlands
    J Pathol 189:12-9. 1999
    ..The extreme rarity of HPV-negative cancers reinforces the rationale for HPV testing in addition to, or even instead of, cervical cytology in routine cervical screening...
  2. ncbi Epidemiologic classification of human papillomavirus types associated with cervical cancer
    Nubia Munoz
    International Agency for Research on Cancer, Lyons, France
    N Engl J Med 348:518-27. 2003
    ..Infection with human papilloma virus (HPV) is the main cause of cervical cancer, but the risk associated with the various HPV types has not been adequately assessed...
  3. pmc The causal relation between human papillomavirus and cervical cancer
    F X Bosch
    Institut Catala d Oncologia, Servei d Epidemiologia i Registre del Cancer, Gran Via Km 2 7 s n 08907 L Hospitalet de Llobregat, 08907 Barcelona, Spain
    J Clin Pathol 55:244-65. 2002
    ..It is the right time for medical societies and public health regulators to consider this evidence and to define its preventive and clinical implications. A comprehensive review of key studies and results is presented...
  4. ncbi Identification of herpesvirus-like DNA sequences in AIDS-associated Kaposi's sarcoma
    Y Chang
    Department of Pathology, College of Physicians and Surgeons, Columbia University, New York, NY 10032
    Science 266:1865-9. 1994
    ..These KS-associated herpesvirus-like (KSHV) sequences appear to define a new human herpesvirus...
  5. ncbi Risk of hepatocellular carcinoma across a biological gradient of serum hepatitis B virus DNA level
    Chien Jen Chen
    Graduate Institute of Epidemiology, College of Public Health, National Taiwan University, Taipei, Taiwan
    JAMA 295:65-73. 2006
    ..Serum hepatitis B virus (HBV) DNA level is a marker of viral replication and efficacy of antiviral treatment in individuals with chronic hepatitis B...
  6. ncbi Worldwide prevalence and genotype distribution of cervical human papillomavirus DNA in women with normal cytology: a meta-analysis
    Silvia de Sanjose
    Servei d Epidemiologia i Registre del Cancer, Institut Catala d Oncologia, Barcelona, Spain
    Lancet Infect Dis 7:453-9. 2007
    ..The HPV types most commonly detected are similar to those most commonly described in pre-neoplastic and cancer cases, although the relative contribution of HPV16 and HPV18 is substantially lower in cytologically normal women...
  7. pmc Presence of an inducible HIV-1 latent reservoir during highly active antiretroviral therapy
    T W Chun
    Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 94:13193-7. 1997
    ..In addition, the presence of unintegrated HIV-1 DNA in infected resting CD4+ T cells from patients receiving HAART, even those with undetectable plasma viremia, suggests persistent active virus replication in vivo...
  8. ncbi A quantitative assay for HIV DNA integration in vivo
    S L Butler
    Infectious Disease Laboratory, The Salk Institute for Biological Studies, La Jolla, California, USA
    Nat Med 7:631-4. 2001
    ..steps of infection by HIV-1 involve entry of the viral core into cells, reverse transcription to form the linear viral DNA, and integration of that DNA into a chromosome of the host...
  9. pmc Hepatitis B virus biology
    C Seeger
    Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA
    Microbiol Mol Biol Rev 64:51-68. 2000
    ..The state of knowledge in this very active field is therefore reviewed with an emphasis on past accomplishments as well as goals for the future...
  10. ncbi Interaction of the bovine papillomavirus E2 protein with Brd4 tethers the viral DNA to host mitotic chromosomes
    Jianxin You
    Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
    Cell 117:349-60. 2004
    ..The interaction of E2 with Brd4 is required to ensure the tethering of viral genomes to the host mitotic chromosomes for persistence of viral episomes in PV-infected cells...
  11. ncbi Human papillomavirus and Papanicolaou tests to screen for cervical cancer
    Pontus Naucler
    Department of Medical Microbiology, Malmo University Hospital, Lund University, Malmo, Sweden
    N Engl J Med 357:1589-97. 2007
    ....
  12. pmc Genomic analysis of uncultured marine viral communities
    Mya Breitbart
    Department of Biology, San Diego State University, San Diego, CA 92182 4614, USA
    Proc Natl Acad Sci U S A 99:14250-5. 2002
    ..Overall, diversity of the viral communities was extremely high. The results also showed that it would be possible to sequence the entire genome of an uncultured marine viral community...
  13. ncbi Against which human papillomavirus types shall we vaccinate and screen? The international perspective
    Nubia Munoz
    International Agency for Research on Cancer, Lyon, France
    Int J Cancer 111:278-85. 2004
    ..The impact of modifying the number of types in the screening cocktail tests would be small and probably irrelevant for screening programs...
  14. pmc Real-time PCR in virology
    Ian M Mackay
    Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children s Hospital, Brisbane, Australia
    Nucleic Acids Res 30:1292-305. 2002
    ..However, the technology discussed has been applied to other areas of microbiology as well as studies of gene expression and genetic disease...
  15. pmc Human immunodeficiency virus type 1 cDNAs produced in the presence of APOBEC3G exhibit defects in plus-strand DNA transfer and integration
    Jean L Mbisa
    HIV Drug Resistance Program, SAIC Frederick, Inc, National Cancer Institute Frederick, Frederick, MD 21702 1201, USA
    J Virol 81:7099-110. 2007
    ..A3G may also inhibit HIV-1 replication by reducing viral DNA synthesis and inducing viral DNA degradation...
  16. pmc Coding potential of laboratory and clinical strains of human cytomegalovirus
    Eain Murphy
    Department of Molecular Biology, Princeton University, Princeton, NJ 80544, USA
    Proc Natl Acad Sci U S A 100:14976-81. 2003
    ..Multiple sequence alignments revealed substantial variation in the amino acid sequences encoded by many of the conserved ORFs...
  17. ncbi Evidence for editing of human papillomavirus DNA by APOBEC3 in benign and precancerous lesions
    Jean Pierre Vartanian
    Molecular Retrovirology Unit, Institut Pasteur, 28 Rue de Docteur Roux, 75724 Paris Cedex 15, France
    Science 320:230-3. 2008
    ..Thus, stochastic or transient overexpression of APOBEC3 genes may expose the genome to a broad spectrum of mutations that could influence the development of tumors...
  18. ncbi Fidelity of HIV-1 reverse transcriptase
    B D Preston
    Department of Pathology, University of Washington, Seattle 98195
    Science 242:1168-71. 1988
    ..The specificity of misincorporation may provide a basis for the systematic construction of antiviral nucleosides...
  19. pmc Identification of a novel Gammaretrovirus in prostate tumors of patients homozygous for R462Q RNASEL variant
    Anatoly Urisman
    Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California, United States of America
    PLoS Pathog 2:e25. 2006
    ..These findings also raise questions about the possible relationship between exogenous infection and cancer development in genetically susceptible individuals...
  20. ncbi Broad antiretroviral defence by human APOBEC3G through lethal editing of nascent reverse transcripts
    Bastien Mangeat
    Department of Genetics and Microbiology, University of Geneva, 1211 Geneva 4, Switzerland
    Nature 424:99-103. 2003
    ..We also find that APOBEC3G can act on a broad range of retroviruses in addition to HIV, suggesting that hypermutation by editing is a general innate defence mechanism against this important group of pathogens...
  21. ncbi Rates of evolutionary change in viruses: patterns and determinants
    Siobain Duffy
    Center for Infectious Disease Dynamics, Department of Biology, Mueller Laboratory, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Nat Rev Genet 9:267-76. 2008
    ....
  22. ncbi The elevated 10-year risk of cervical precancer and cancer in women with human papillomavirus (HPV) type 16 or 18 and the possible utility of type-specific HPV testing in clinical practice
    Michelle J Khan
    Division of Cancer Epidemiology and Genetics, National Cancer Institute, 6120 Executive Blvd, Rm 7074, Rockville, MD 20852, USA
    J Natl Cancer Inst 97:1072-9. 2005
    ....
  23. ncbi Human papillomavirus types in head and neck squamous cell carcinomas worldwide: a systematic review
    Aimee R Kreimer
    International Agency for Research on Cancer, Lyon, France
    Cancer Epidemiol Biomarkers Prev 14:467-75. 2005
    ..Small sample size and publication bias complicate the assessment of the prevalence of HPV in head and neck sites beyond the oropharynx...
  24. pmc Antiviral potency of APOBEC proteins does not correlate with cytidine deamination
    Kate N Bishop
    Department of Infectious Diseases, King s College London School of Medicine, Guy s Hospital, London Bridge, UK
    J Virol 80:8450-8. 2006
    ..In other words, the antiviral phenotype of APOBEC proteins correlates with their ability to prevent the accumulation of reverse transcripts and not with the induction of hypermutation...
  25. pmc Cytidine deaminases APOBEC3G and APOBEC3F interact with human immunodeficiency virus type 1 integrase and inhibit proviral DNA formation
    Kun Luo
    Department of Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, 615 N Wolfe Street, Baltimore, MD 21205, USA
    J Virol 81:7238-48. 2007
    ..Thus, multiple steps of the HIV-1 replication cycle, most noticeably the formation of proviral DNA, are inhibited by both cytidine deamination-dependent and -independent mechanisms...
  26. ncbi The 1.2-megabase genome sequence of Mimivirus
    Didier Raoult
    Unite des Rickettsies, Faculte de Medecine, CNRS UMR6020, Universite de la Mediterranee, 13385 Marseille Cedex 05, France
    Science 306:1344-50. 2004
    ..This new sequence data might help shed a new light on the origin of DNA viruses and their role in the early evolution of eukaryotes...
  27. pmc Molecular characterization of preintegration latency in human immunodeficiency virus type 1 infection
    Theodore C Pierson
    Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    J Virol 76:8518-31. 2002
    ..The delay in completing reverse transcription may make the viral DNA genome more susceptible to competing decay processes...
  28. ncbi Inhibition of hepatitis B virus replication by APOBEC3G
    Priscilla Turelli
    Department of Microbiology and Molecular Medicine and Frontiers in Genetics Research Program, University of Geneva, Switzerland
    Science 303:1829. 2004
  29. pmc Evolutionary relationships among diverse bacteriophages and prophages: all the world's a phage
    R W Hendrix
    Pittsburgh Bacteriophage Institute, Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Proc Natl Acad Sci U S A 96:2192-7. 1999
    ....
  30. pmc A base-excision DNA-repair protein finds intrahelical lesion bases by fast sliding in contact with DNA
    Paul C Blainey
    Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 103:5752-7. 2006
    ..5 kcal/mol (1 kcal = 4.2 kJ). This nearly barrierless Brownian sliding indicates that DNA glycosylases locate lesion bases by a massively redundant search in which the enzyme selectively binds 8-oxoguanine under kinetic control...
  31. pmc Synthesis of infectious human papillomavirus type 18 in differentiating epithelium transfected with viral DNA
    C Meyers
    Department of Microbiology and Immunology, The Pennsylvania State University College of Medicine, Hershey 17033, USA
    J Virol 71:7381-6. 1997
    ..has permitted the study of a number of the differentiation-specific aspects of HPV, including amplification of viral DNA, expression of late genes, and viral morphogenesis...
  32. ncbi HIV-1 integration in the human genome favors active genes and local hotspots
    Astrid R W Schroder
    Infectious Disease Laboratory, The Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Cell 110:521-9. 2002
    ..4 kb region containing 1% of sites. These data document unexpectedly strong biases in integration site selection and suggest how selective targeting promotes aggressive HIV replication...
  33. ncbi Inhibitors of strand transfer that prevent integration and inhibit HIV-1 replication in cells
    D J Hazuda
    Department of Antiviral Research, Merck Research Laboratories, West Point, PA 19486, USA
    Science 287:646-50. 2000
    ..The antiviral activity of these compounds is due exclusively to inhibition of one of the two catalytic functions of integrase, strand transfer...
  34. ncbi Homologous recombination between different genotypes of hepatitis B virus
    V Morozov
    Influenza Institute, Russian Academy of Medical Science, Prof Popov Str 15 17, 197376, St Petersburg, Russia
    Gene 260:55-65. 2000
    ..A possible mechanism and the implications of recombination for the natural history of HBV, clinically important properties, and phylogenetic reconstruction are discussed...
  35. ncbi Effect of treatment, during primary infection, on establishment and clearance of cellular reservoirs of HIV-1
    Matthew C Strain
    Department of Physics, University of California at San Diego, La Jolla, California, USA
    J Infect Dis 191:1410-8. 2005
    ..1 IUPM; P<.0005). These results suggest that treatment <6 months after seroconversion may facilitate long-term control of cellular reservoirs that maintain HIV-1 infection during treatment...
  36. ncbi Occult hepatitis B
    Michael Torbenson
    Department of Pathology, Division of Gastrointestinal and Hepatic Pathology, Johns Hopkins Hospital, Baltimore, MD, USA
    Lancet Infect Dis 2:479-86. 2002
    ..Although much more research is needed, existing reports justify a heightened awareness of the medical importance and means of testing for occult hepatitis B...
  37. ncbi Prevalence of HPV infection among females in the United States
    Eileen F Dunne
    Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    JAMA 297:813-9. 2007
    ..Baseline population prevalence data for HPV infection in the United States before widespread availability of a prophylactic HPV vaccine would be useful...
  38. ncbi Adefovir dipivoxil for the treatment of hepatitis B e antigen-positive chronic hepatitis B
    Patrick Marcellin
    Service d Hepatologie, INSERM Unité 481, and Centre de Recherches Claude Bernard sur les Hépatites Virales, Hopital Beaujon, Clichy, France
    N Engl J Med 348:808-16. 2003
    ..In preclinical and phase 2 studies, adefovir dipivoxil demonstrated potent activity against hepatitis B virus (HBV), including lamivudine-resistant strains...
  39. pmc Retroviral DNA integration: viral and cellular determinants of target-site selection
    Mary K Lewinski
    Infectious Disease Laboratory, The Salk Institute, La Jolla, California, USA
    PLoS Pathog 2:e60. 2006
    Retroviruses differ in their preferences for sites for viral DNA integration in the chromosomes of infected cells...
  40. ncbi The detection of monkeypox in humans in the Western Hemisphere
    Kurt D Reed
    Department of Pathology, Marshfield Clinic, Marshfield, Wisc, USA
    N Engl J Med 350:342-50. 2004
    ..Zoonotic transmission of a bacterial or viral pathogen was suspected...
  41. ncbi Isolation, cloning, and sequencing of simian foamy viruses from chimpanzees (SFVcpz): high homology to human foamy virus (HFV)
    O Herchenröder
    Department of Medical Pathology, University of California, Davis 95616
    Virology 201:187-99. 1994
    ..Based on sequence comparisons in this report, primate foamy viruses may be arranged into different clusters with SFVcpz and HFV forming one cluster and SFV-1 and SFV-3 as prototypes for two unique clusters...
  42. pmc Clustered mutations in HIV-1 gag are consistently required for escape from HLA-B27-restricted cytotoxic T lymphocyte responses
    A D Kelleher
    Medical Research Council Human Immunology Unit, Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford OX3 9DS, United Kingdom
    J Exp Med 193:375-86. 2001
    ..Outgrowth of CTL escape viruses required high viral loads and additional, possibly compensatory, mutations in the gag protein...
  43. ncbi Identification of a novel coronavirus in patients with severe acute respiratory syndrome
    Christian Drosten
    Bernhard Nocht Institute for Tropical Medicine, National Reference Center for Tropical Infectious Diseases, Hamburg, Germany
    N Engl J Med 348:1967-76. 2003
    ..The severe acute respiratory syndrome (SARS) has recently been identified as a new clinical entity. SARS is thought to be caused by an unknown infectious agent...
  44. ncbi Human Merkel cell polyomavirus infection I. MCV T antigen expression in Merkel cell carcinoma, lymphoid tissues and lymphoid tumors
    Masahiro Shuda
    Molecular Virology Program, University of Pittsburgh Cancer Institute, Pittsburgh, PA 15213, USA
    Int J Cancer 125:1243-9. 2009
    ..As with some of the other human polyomaviruses, lymphocytes may serve as a tissue reservoir for MCV infection, but hematolymphoid malignancies associated with MCC are unlikely to be caused by MCV...
  45. pmc Analysis of early human immunodeficiency virus type 1 DNA synthesis by use of a new sensitive assay for quantifying integrated provirus
    Audrey Brussel
    Departement des Maladies Infectieuses, Institut Cochin, INSERM U567, CNRS UMR 8104, Universite Rene Descartes, 22 rue Mechain, 75014 Paris, France
    J Virol 77:10119-24. 2003
    ..Parallel assays for total HIV-1 DNA and two-LTR HIV-1 DNA circles allowed the synthesis and fate of the different HIV-1 DNA species to be monitored upon a single round of viral replication...
  46. ncbi Integration and excision by the large serine recombinase phiRv1 integrase
    Lori A Bibb
    Pittsburgh Bacteriophage Institute and Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Mol Microbiol 55:1896-910. 2005
    ....
  47. pmc Intersubunit coordination in a homomeric ring ATPase
    Jeffrey R Moffitt
    Department of Physics and Jason L Choy Laboratory of Single Molecule Biophysics, University of California, Berkeley, California 94720, USA
    Nature 457:446-50. 2009
    ..Furthermore, a step size that is a non-integer number of base pairs demands new models for motor-DNA interactions...
  48. pmc Herpes simplex virus ICP0 promotes both histone removal and acetylation on viral DNA during lytic infection
    Anna R Cliffe
    Harvard Medical School, Department of Microbiology and Molecular Genetics, 200 Longwood Avenue, Boston, MA 02115, USA
    J Virol 82:12030-8. 2008
    ..The proportion of viral DNA associated with histone H3 decreases at later times postinfection, independently of either viral DNA replication ..
  49. pmc Nuclear import of HIV-1 intracellular reverse transcription complexes is mediated by importin 7
    Ariberto Fassati
    The Wohl Virion Centre, Windeyer Institute, University College London Medical School, 46 Cleveland Street, London W1T 4JF, UK
    EMBO J 22:3675-85. 2003
    ..These results provide a new insight into the molecular mechanism for HIV-1 nuclear import and reveal potential targets for therapeutic intervention...
  50. pmc Forces and pressures in DNA packaging and release from viral capsids
    Shelly Tzlil
    Department of Physical Chemistry and the Fritz Haber Research Center, The Hebrew University, Jerusalem, Israel
    Biophys J 84:1616-27. 2003
    ..The results are interpreted in terms of the (competing) bending and interaction components of the packing energy, and are shown to be in good agreement with available experimental data...
  51. pmc Longitudinal analysis of CD8+ T cells specific for structural and nonstructural hepatitis B virus proteins in patients with chronic hepatitis B: implications for immunotherapy
    George J M Webster
    Institute of Hepatology, University College London, 69 75 Chenies Mews, London WC1E 6HX, United Kingdom
    J Virol 78:5707-19. 2004
    ..These findings have implications for the design of immunotherapy for chronic HBV infections...
  52. pmc Kinetics of human immunodeficiency virus type 1 decay following entry into resting CD4+ T cells
    Yan Zhou
    Johns Hopkins University School of Medicine, 720 Rutland Ave, Baltimore, MD 21205, USA
    J Virol 79:2199-210. 2005
    ..Therefore, the reservoir of unintegrated HIV-1 in recently infected resting CD4(+) T cells is highly labile...
  53. pmc The generalized transducing Salmonella bacteriophage ES18: complete genome sequence and DNA packaging strategy
    Sherwood R Casjens
    Department of Pathology, University of Utah Medical School, Salt Lake City, UT 84132, USA
    J Bacteriol 187:1091-104. 2005
    ..Bioinformatic analysis of large terminase subunits shows that the different functional classes of phage-encoded terminases can usually be predicted from their amino acid sequence...
  54. ncbi Human bocavirus: a novel parvovirus epidemiologically associated with pneumonia requiring hospitalization in Thailand
    Alicia M Fry
    Epidemiology Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    J Infect Dis 195:1038-45. 2007
    ..We detected human bocavirus (HBoV) infection in 4.5% of hospitalized patients with pneumonia in rural Thailand. However, the role of HBoV as a pathogen is unclear...
  55. ncbi Subviral agents associated with plant single-stranded DNA viruses
    R W Briddon
    Plant Biotechnology Division, National Institute for Biotechnology and Genetic Engineering, Jhang Road, Faisalabad, Pakistan
    Virology 344:198-210. 2006
    ..A greater understanding of how they elicit the host response may provide useful information for their control as well as an insight into plant developmental processes...
  56. ncbi Rapid kinetics of protein-nucleic acid interaction is a major component of HIV-1 nucleocapsid protein's nucleic acid chaperone function
    Margareta Cruceanu
    Department of Physics, Northeastern University, Boston, MA 02115, USA
    J Mol Biol 363:867-77. 2006
    ..Taken together, these observations establish the rapid kinetics of protein-nucleic acid interaction as another major component of NC's chaperone function...
  57. pmc Weak palindromic consensus sequences are a common feature found at the integration target sites of many retroviruses
    Xiaolin Wu
    Laboratory of Molecular Technology, Scientific Application International Corporation, National Cancer Institute at Frederick, 915 Toll House Ave, Frederick, MD 21701, USA
    J Virol 79:5211-4. 2005
    ..While integrase has strict sequence requirements for the viral DNA ends, target site sequences have been shown to be very diverse...
  58. ncbi A molecular analysis of prokaryotic and viral DNA sequences in prostate tissue from patients with prostate cancer indicates the presence of multiple and diverse microorganisms
    Karen S Sfanos
    Department of Urology, Johns Hopkins Medical Institutions, Baltimore, Maryland, USA
    Prostate 68:306-20. 2008
    ..Infectious pathogens are potential stimuli for prostatic inflammation...
  59. pmc Evidence for a functional link between uncoating of the human immunodeficiency virus type 1 core and nuclear import of the viral preintegration complex
    David J Dismuke
    Department of Microbiology and Immunology, Vanderbilt University School of Medicine, A 5301 Medical Center North, Nashville, Tennessee 37232 2363, USA
    J Virol 80:3712-20. 2006
    ..two substitutions in CA (Q63A/67A) was unusual in that it was poorly infectious yet synthesized normal levels of viral DNA. Here we report that this mutant is impaired for nuclear entry...
  60. ncbi Viral DNA load, physical status and E2/E6 ratio as markers to grade HPV16 positive women for high-grade cervical lesions
    Monica Cricca
    Department of Clinical and Experimental Medicine, Microbiology Section, University of Bologna, and Department of Obstetrics and Gynecology, S Orsola Malpighi Hospital, Italy
    Gynecol Oncol 106:549-57. 2007
    ..This study has evaluated the significance of viral markers, such as viral load, physical status and E2/E6 ratio, to stratify HPV16 infected women at a single point in time for grade of cervical lesions...
  61. ncbi Hepatitis B viral DNA is methylated in liver tissues
    P Vivekanandan
    Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    J Viral Hepat 15:103-7. 2008
    ..In conclusion, HBV-DNA can be methylated in human tissues and methylation may play an important role in regulation of HBV gene expression...
  62. pmc Herpes simplex virus-infected cell protein 0 blocks the silencing of viral DNA by dissociating histone deacetylases from the CoREST-REST complex
    Haidong Gu
    Marjorie B Kovler Viral Oncology Laboratories, University of Chicago, 910 East 58th Street, Chicago, IL 60637, USA
    Proc Natl Acad Sci U S A 104:17134-9. 2007
    ..We conclude that CoREST(146-482) functionally replaced ICP0 and that, by extension, ICP0 acts to block the silencing of viral DNA by displacing HDAC1/2 from the CoREST-REST complex.
  63. ncbi A role for DNA-PK in retroviral DNA integration
    R Daniel
    Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, PA 19111, USA
    Science 284:644-7. 1999
    ..These results suggest that the initial events in retroviral integration are detected as DNA damage by the host cell and that completion of the integration process requires the DNA-PK-mediated repair pathway...
  64. ncbi Polydnavirus genomes reflect their dual roles as mutualists and pathogens
    Bruce A Webb
    Department of Entomology, University of Kentucky, Lexington, KY 40506, USA
    Virology 347:160-74. 2006
    ....
  65. pmc Massive APOBEC3 editing of hepatitis B viral DNA in cirrhosis
    Jean Pierre Vartanian
    Molecular Retrovirology Unit, Institut Pasteur, Paris, France
    PLoS Pathog 6:e1000928. 2010
    ..Although the mutant spectrum resulting from A3 editing is highly deleterious, a very small part, notably the lightly edited genomes, might help the virus evolve and even escape immune responses...
  66. pmc Simian immunodeficiency virus-infected macaques treated with highly active antiretroviral therapy have reduced central nervous system viral replication and inflammation but persistence of viral DNA
    M Christine Zink
    Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    J Infect Dis 202:161-70. 2010
    ..During the era of highly active antiretroviral therapy (HAART), the prevalence of HIV-associated central nervous system (CNS) disease has increased despite suppression of plasma viremia...
  67. pmc Integrated human papillomavirus type 16 is frequently found in cervical cancer precursors as demonstrated by a novel quantitative real-time PCR technique
    Panu Peitsaro
    Department of Oral Pathology and Oral Radiology and MediCity Research Laboratory, Faculty of Medicine, University of Turku, Turku, Finland
    J Clin Microbiol 40:886-91. 2002
    ..Thus, the method described here is a very sensitive tool with which to assess the physical state of HPV, which is useful in predicting disease progression...
  68. ncbi Prevalence and type spectrum of human papillomaviruses in healthy skin samples collected in three continents
    Annika Antonsson
    Department of Medical Microbiology, Malmo University Hospital, Lund University, SE 20502 Malmo, Sweden
    J Gen Virol 84:1881-6. 2003
    ..5 %. This was also the only type that was found in samples from all of the countries in the study. The results presented show that commensal skin HPV infections have a worldwide distribution with a very broad spectrum of genotypes...
  69. ncbi Human papillomavirus genotype distribution in low-grade cervical lesions: comparison by geographic region and with cervical cancer
    Gary M Clifford
    IARC, 150 cours Albert Thomas, F 69372 Lyon Cedex 08, France
    Cancer Epidemiol Biomarkers Prev 14:1157-64. 2005
    ..Some regional differences in the relative importance of HPV genotypes in LSIL were noted...
  70. ncbi CD4 cell count and HIV DNA level are independent predictors of disease progression after primary HIV type 1 infection in untreated patients
    Cecile Goujard
    Service de Medecine Interne, Bicetre Hospital, Assistance Publique Hopitaux de Paris AP HP, Paris, France
    Clin Infect Dis 42:709-15. 2006
    ..Treatment initiation at the time of primary human immunodeficiency virus (HIV) type 1 (HIV-1) infection has become less frequent in recent years...
  71. ncbi Relationship of genotypes of hepatitis B virus to mutations, disease progression and response to antiviral therapy
    A Kramvis
    MRC University Molecular Hepatology Research Unit, Department of Medicine, University of the Witwatersrand, Johannesburg, South Africa
    J Viral Hepat 12:456-64. 2005
    ....
  72. pmc Identification of a third human polyomavirus
    Tobias Allander
    Laboratory for Clinical Microbiology, Department of Clinical Microbiology, Tumor and Cell Biology, L2 02, Karolinska University Hospital, SE 171 76 Stockholm, Sweden
    J Virol 81:4130-6. 2007
    ..This finding further illustrates how unbiased screening of respiratory tract samples can be used for the discovery of diverse virus types...
  73. pmc Widely conserved recombination patterns among single-stranded DNA viruses
    P LeFeuvre
    CIRAD, UMR 53 PVBMT CIRAD Université de La Réunion, Pole de protection des plantes, Ligne Paradis, 97410 Saint Pierre, La Reunion, France
    J Virol 83:2697-707. 2009
    ..Collectively, these results imply that natural selection acting against viruses expressing recombinant proteins is a major determinant of nonrandom recombination breakpoint distributions observable in most ssDNA virus families...
  74. pmc The cytidine deaminase CEM15 induces hypermutation in newly synthesized HIV-1 DNA
    Hui Zhang
    The Dorrance H Hamilton Laboratories, Center for Human Virology and Biodefense, Division of Infectious Diseases and Environmental Medicine, Department of Medicine, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Nature 424:94-8. 2003
    ..1 (HIV-1) replication, is a cytidine deaminase and is able to induce G to A hypermutation in newly synthesized viral DNA. This effect can be counteracted by the HIV-1 virion infectivity factor (Vif)...
  75. pmc Hydrodynamic injection of viral DNA: a mouse model of acute hepatitis B virus infection
    Priscilla L Yang
    Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 99:13825-30. 2002
    ..In addition, this methodology represents a prototype for the study of other known and to-be-discovered liver-specific pathogens...
  76. ncbi Hepatitis B virus genotype and DNA level and hepatocellular carcinoma: a prospective study in men
    Ming Whei Yu
    Department of Public Health, Graduate Institute of Epidemiology, College of Public Health, National Taiwan University, Taipei, Taiwan
    J Natl Cancer Inst 97:265-72. 2005
    ..Although chronic infection with hepatitis B virus (HBV) has been established as a cause of hepatocellular carcinoma (HCC), the roles of viral load and HBV genotype remain unclear...
  77. ncbi The orientation of mycobacteriophage Bxb1 integration is solely dependent on the central dinucleotide of attP and attB
    Pallavi Ghosh
    Pittsburgh Bacteriophage Institute and Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Mol Cell 12:1101-11. 2003
    ....
  78. pmc A virus discovery method incorporating DNase treatment and its application to the identification of two bovine parvovirus species
    T Allander
    Sections for Hepatitis Viruses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 98:11609-14. 2001
    ..DNase treatment of serum samples may prove to be a very useful tool for virus discovery. The DNase-SISPA method is suitable for screening of a large number of samples and also enables rapid sequence determination of high-titer viruses...
  79. pmc Human bocavirus infections in hospitalized children and adults
    Jean Longtin
    Centre Hospitalier Universitaire de Quebec, Quebec City, Quebec, Canada
    Emerg Infect Dis 14:217-21. 2008
    ..Two clades of HBoV were identified. The pathogenic role of HBoV in RTIs is uncertain because it was frequently detected in symptomatic and asymptomatic children and was commonly found with other viruses in symptomatic children...
  80. pmc Human papillomavirus type 16 integration in cervical carcinoma in situ and in invasive cervical cancer
    Hugo Arias-Pulido
    Department of Molecular Genetics and Microbiology, School of Medicine, Health Sciences Center, University of New Mexico, Albuquerque, NM 87111, USA
    J Clin Microbiol 44:1755-62. 2006
    ..HPV-16 E2 expressed from intact episomes may act in trans to regulate integrated genome expression of E6 and E7...
  81. pmc Real-time PCR-based system for simultaneous quantification of human papillomavirus types associated with high risk of cervical cancer
    Martin Moberg
    Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, SE 751 85 Uppsala, Sweden
    J Clin Microbiol 41:3221-8. 2003
    ..In order to study the relationship between viral DNA amount and risk of cervical carcinoma for the HPV types most commonly found in cervical tumors, we developed a ..
  82. pmc Genetic editing of HBV DNA by monodomain human APOBEC3 cytidine deaminases and the recombinant nature of APOBEC3G
    Michel Henry
    Molecular Retrovirology Unit, CNRS URA 3015, Institut Pasteur, Paris, France
    PLoS ONE 4:e4277. 2009
    ..A phylogenic analysis of A3 exons revealed that A3G is in fact a chimera with the first two exons being derived from the A3F gene. This might allow co-expression of the two genes that are able to restrict HIV-1Deltavif efficiently...
  83. pmc The early autophagic pathway is activated by hepatitis B virus and required for viral DNA replication
    Donna Sir
    Department of Molecular Microbiology and Immunology, University of Southern California, Los Angeles, CA 90033, USA
    Proc Natl Acad Sci U S A 107:4383-8. 2010
    ..Our studies thus demonstrate that a DNA virus can use autophagy to enhance its own replication and indicate the possibility of targeting the autophagic pathway for the treatment of HBV patients...
  84. ncbi Persistent human papillomavirus infection as a predictor of cervical intraepithelial neoplasia
    N F Schlecht
    Department of Oncology, McGill University, 546 Pine Ave W, Montreal, Quebec, Canada H2W1S6
    JAMA 286:3106-14. 2001
    ....
  85. pmc Molecular properties, biology, and clinical implications of TT virus, a recently identified widespread infectious agent of humans
    M Bendinelli
    Virology Section, Department of Biomedicine and Retrovirus Center, University of Pisa, Pisa, Italy
    Clin Microbiol Rev 14:98-113. 2001
    ..The virus has not yet been etiologically linked to any other human disease. Thus, TTV should be considered an orphan virus...
  86. pmc Viral complementation allows HIV-1 replication without integration
    Huub C Gelderblom
    Department of Basic Sciences and Craniofacial Biology, New York University College of Dentistry, New York, NY, USA
    Retrovirology 5:60. 2008
    ..Here we examine the influence of an integrated provirus on unintegrated HIV-1 DNA (uDNA)...
  87. pmc Real-time quantitative PCR assays for detection and monitoring of pathogenic human viruses in immunosuppressed pediatric patients
    F Watzinger
    Division of Molecular Microbiology and Development of Genetic Diagnostics, Children s Cancer Research Institute, Vienna, Austria
    J Clin Microbiol 42:5189-98. 2004
    ....
  88. ncbi Herpes simplex virus (HSV) suppression with valacyclovir reduces rectal and blood plasma HIV-1 levels in HIV-1/HSV-2-seropositive men: a randomized, double-blind, placebo-controlled crossover trial
    Richard A Zuckerman
    Section of Infectious Disease and International Health, Dartmouth Hitchcock Medical Center, Lebanon, NH, USA
    J Infect Dis 196:1500-8. 2007
    ..We studied HIV-1 levels during HSV suppression in coinfected persons in a placebo-controlled crossover trial...
  89. ncbi Community genomics among stratified microbial assemblages in the ocean's interior
    Edward F DeLong
    Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Science 311:496-503. 2006
    ..Comparative genomic analyses of stratified microbial communities have the potential to provide significant insight into higher-order community organization and dynamics...
  90. ncbi Pre-S deletion and complex mutations of hepatitis B virus related to advanced liver disease in HBeAg-negative patients
    Chien Hung Chen
    Division of Hepatogastroenterology, Department of Internal Medicine, Chang Gung Memorial Hospital Kaohsiung Medical Center, Kaohsiung, Taiwan
    Gastroenterology 133:1466-74. 2007
    ....
  91. pmc Exploring the mycobacteriophage metaproteome: phage genomics as an educational platform
    Graham F Hatfull
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    PLoS Genet 2:e92. 2006
    ....
  92. ncbi Identification of a proliferation gene cluster associated with HPV E6/E7 expression level and viral DNA load in invasive cervical carcinoma
    Christophe Rosty
    Departement de Biologie des Tumeurs, Institut Curie, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    Oncogene 24:7094-104. 2005
    ..E6/E7 mRNA expression level was positively correlated with the expression level of the cluster genes and with viral DNA load...
  93. pmc The latency-associated nuclear antigen of Kaposi's sarcoma-associated herpesvirus supports latent DNA replication in dividing cells
    Jianhong Hu
    Division of Hematology Oncology and Department of Molecular Biology and Microbiology, Case Western Reserve University, Cleveland, Ohio 44106, USA
    J Virol 76:11677-87. 2002
    ..In addition, LANA is required for maintenance of the episomal viral DNA during latency in dividing cells...
  94. pmc HIV-1 DNA Flap formation promotes uncoating of the pre-integration complex at the nuclear pore
    Nathalie J Arhel
    Groupe de Virologie Moléculaire et Vectorologie, CNRS URA 3015, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France
    EMBO J 26:3025-37. 2007
    ..These data show that DNA Flap formation, the very last event of HIV-1 RT degrees, acts as a viral promoting element for the uncoating of HIV-1 at the nuclear pore...
  95. ncbi Comparisons of HPV DNA detection by MY09/11 PCR methods
    Philip E Castle
    Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, Maryland 20892, USA
    J Med Virol 68:417-23. 2002
    ..Our data also suggest that studies reporting HPV DNA detection by PCR need to report the type of polymerase used, as well as other assay specifics, and underscore the need for worldwide standards of testing...
  96. ncbi Human bocavirus in children hospitalized for acute gastroenteritis: a case-control study
    Wei Xia Cheng
    National Institute for Viral Disease Control and Prevention, China Center for Disease Control and Prevention, Beijing, China
    Clin Infect Dis 47:161-7. 2008
    ..The causative role of HBoV in human gastroenteritis remains uncertain, and, to our knowledge, no previous case-control study has studied the relationship between HBoV and gastroenteritis...
  97. ncbi Comparison of tissue distribution, persistence, and molecular epidemiology of parvovirus B19 and novel human parvoviruses PARV4 and human bocavirus
    Ashleigh Manning
    Centre for Infectious Diseases, Western General Hospital, University of Edinburgh, Edinburgh, EH9 1QH, United Kingdom
    J Infect Dis 195:1345-52. 2007
    ..We investigated the frequencies of persistence, tissue distribution, and influence of immunosuppression on replication of these viruses...
  98. pmc Integration of human immunodeficiency virus type 1 DNA in vitro
    C M Farnet
    Dana Farber Cancer Institute, Department of Pathology, Harvard Medical School, Boston, MA 02115
    Proc Natl Acad Sci U S A 87:4164-8. 1990
    ..Linear viral DNA synthesis occurs in the cytoplasm of newly infected cells, reaching peak levels 4 hr after infection...
  99. ncbi High prevalence of human cytomegalovirus in prostatic intraepithelial neoplasia and prostatic carcinoma
    Minu Samanta
    Surgical Service, Veterans Affairs Medical Center, University of Alabama at Birmingham, Birmingham, AL, USA
    J Urol 170:998-1002. 2003
    ..Based on increased awareness of the oncogenic potential of this virus, we decided to reexplore the issue of whether HCMV might be involved in prostate cancer pathogenesis...
  100. ncbi Genetic patterns in head and neck cancers that contain or lack transcriptionally active human papillomavirus
    Boudewijn J M Braakhuis
    Department of Otolaryngology Head and Neck Surgery, VU University Medical Center, Amsterdam, The Netherlands
    J Natl Cancer Inst 96:998-1006. 2004
    ..We tested the hypothesis that HPV-associated HNSCCs display a pattern of genetic alterations different from those of HNSCCs without HPV DNA...
  101. ncbi Application of viral-load kinetics to identify patients who develop cytomegalovirus disease after transplantation
    V C Emery
    Department of Virology, Royal Free and University College Medical School, London, UK
    Lancet 355:2032-6. 2000
    ..Peak CMV load in the blood correlates with CMV disease but frequently occurs too late to provide prognostic information...

Research Grants71

  1. Regulation of HSV-1 Gene Expression and Replication by Nuclear Hormone Receptors
    SHAOCHUNG VICTOR HSIA; Fiscal Year: 2013
    ..is supported by the literature and our observations: First, HSV-1 TK and ICP0 are important for efficient viral DNA replication and transcription within the infected neurons. Second, TR is present in sensory ganglia...
  2. Molecular Mechanisms of Parvovirus Gene Expression and Replication
    Jianming Qiu; Fiscal Year: 2013
    ..mediated by the erythropoietin receptor to the Ras/MEK/ERK pathway in EPCs, and plays an important role in viral DNA replication. Importantly, B19V infection induces a DNA damage response (DDR)...
  3. Hepatitis B virus replication and DDB1
    Betty L Slagle; Fiscal Year: 2013
    ..The role of HBV in mediating carcinogenesis is complex and includes integration of viral DNA into host chromosomes, the host immune system, and expression of the viral regulatory HBx protein...
  4. Regulation of EBV Latency by EBNA1
    Paul M Lieberman; Fiscal Year: 2013
    ..EBNA1 is a multifunctional, sequence-specific DNA-binding protein that regulates viral DNA replication, episome maintenance, metaphase chromosome tethering, and transcription...
  5. Nanomedicine and NeuroAIDS
    Howard E Gendelman; Fiscal Year: 2013
    ..First, we seek to maximize viral clearance (measured reduction of integrated viral DNA) by developing a range of antiretroviral nanoformulated drugs used alone or in combination...
  6. Biophysics of Drug Interactions with Viral Episomes
    James K Bashkin; Fiscal Year: 2013
    ..The proposed work determines the biophysics of interactions between antiviral polyamides (PAs) and their viral DNA targets, in order to determine the mechanism of action...
  7. E2-Cellular Complexes in HPV Chromatin Transcription
    Cheng Ming Chiang; Fiscal Year: 2013
    ..Since early protein 2 (E2) encoded by HPVs is crucial for viral DNA replication, HPV genome segregation, control of viral gene expression, and induction of cellular apoptosis and ..
  8. Agnoprotein in JC virus virion biogenesis and replication
    Mahmut Safak; Fiscal Year: 2012
    ..the molecular mechanisms by which agnoprotein regulates both the virion formation and large T antigen-mediated viral DNA replication;and ii) examine the impact of both PP2A and Sm t-Ag on agnoprotein functions during this process...
  9. Encapsidation of the poxvirus genome
    Paula Traktman; Fiscal Year: 2012
    ..b>Viral DNA synthesis, which is accomplished by a repertoire of virally encoded proteins, is followed by the processing of ..
  10. Genetic Epidemiology of Life-Threatening Influenza in Children
    Adrienne G Randolph; Fiscal Year: 2013
    ..We are in the midst of an influenza pandemic caused by a novel H1N1 influenza strain that includes swine origin viral DNA. The 2009 Pandemic H1N1 influenza strain is causing excess morbidity and mortality in children and young adults...
  11. Characterization of a novel rabbit herpes simplexvirus: a possible model for unde
    LING J JIN; Fiscal Year: 2010
    ..PCR using degenerative primers to conserved herpesvirus genes was used to amplify sequences from purified viral DNA. Sequencing of these products allowed the design of specific primers so that complete sequence data for a number ..
  12. Mechanisms of Viral DNA Packaging
    CARLOS JOSE BUSTAMANTE; Fiscal Year: 2013
    ..Finally, we wish to investigate how the increasing internal DNA pressure regulates the dynamics of the motor during packaging. ..
  13. MECHANISMS OF HUMAN PAPILLOMAVIRUS DNA REPLICATION
    Louise T Chow; Fiscal Year: 2013
    ..pocket proteins) in the differentiated keratinocytes, enabling the S phase reentry and progression necessary for viral DNA amplification...
  14. SPORE in Lymphoma
    Richard Ambinder; Fiscal Year: 2009
    ..Epstein-Barr virus (EBV) is present in the malignant cells of HL in many patients. Measurement of viral DNA may be used in tumor monitoring...
  15. GENETICS OF HERPESVIRUS TRANSFORMATION
    David M Knipe; Fiscal Year: 2013
    ..In this application our specific aims are to: 1. Define the mechanisms of chromatin regulation on viral DNA through targeting of the viral genome to the nuclear lamina in studies to determine the viral and host gene ..
  16. Mechanism of CCC DNA Synthesis in Hepatitis B Virus
    Christoph Seeger; Fiscal Year: 2013
    ..However, the mechanism responsible for the conversion of the relaxed circular (RC) viral DNA genome into covalently closed circular (CCC) DNA, the template for viral RNA transcription, is not yet known...
  17. Gene Expression in HSV-1 Latency After Corneal Infection
    Paul R Kinchington; Fiscal Year: 2013
    ..Our first specific aim tests the hypothesis that CD8+T cell recognition of viral antigens made before viral DNA replication is critical to effectively block reactivation in a non-cytolytic manner...
  18. Fanconi Anemia and HPV Transformation
    Susanne I Wells; Fiscal Year: 2013
    ..is the predominant oncogene, stimulates cell proliferation in differentiated epidermis, and is required for viral DNA replication...
  19. Papillomavirus E2 Functions: Cellular Regulation and Effectors
    Peter M Howley; Fiscal Year: 2013
    ..for vira DNA replication as an auxiliary DNA replication factor that helps recruit the viral E1 helicase to the viral DNA replication origin...
  20. The in vivo function of A3A and A3G during retrovirus infection
    Spyridon Stavrou; Fiscal Year: 2013
    ..and can convert cytidines to uridines in reverse-transcribed single-stranded DNA, resulting in mutation of the viral DNA. While the human A3 proteins have been extensively studied in tissue culture, little is known about their action ..
  21. Screens for host DNA replication and repair factors involved in viral infection
    Matthew D Weitzman; Fiscal Year: 2010
    ..In vitro replication systems have been useful for studying viral DNA replication mechanisms, but they fail to capture the full complexity of the cellular environment and the ..
  22. The Viral Life Cycle of Human Endogenous Retrovirus Type K
    Derek Dube; Fiscal Year: 2013
    ..Rather, we have found HERV-K particles from both tissue culture cell lines and patient plasma that contain viral DNA genomes, rather than the canonical RNA genomes, suggesting a life cycle more similar to the Spumavirus genus...
  23. CHARACTERIZATION OF HCMV UL84
    Gregory S Pari; Fiscal Year: 2013
    ..has emerged as the central factor proposed to be involved in the regulation and coordination of initiation of viral DNA synthesis...
  24. Genetic analysis of Herpes virus neurotropism and encephalitis
    JENNIFER HART LAVAIL; Fiscal Year: 2011
    ..the HSV viral protein, Us9, is necessary for the long distance spread specifically of viral nucleocapsid and viral DNA from an infected neuron cell body toward the axon terminal...
  25. Intervention of HBV DNA synthesis and transcription
    Aleem Siddiqui; Fiscal Year: 2013
    ..have previously shown that the acyclic nucleoside phosphonates, HPMPC and HPMPA are incorporated into viral DNA. Viral DNA templates containing these bases cannot be copied readily and have aberrations in DNA structure...
  26. Release and trafficking of JC polyomavirus genomes and implications in AIDS
    CHRISTIAN DOUGLAS STUART NELSON; Fiscal Year: 2012
    ..Up to 39% of the population is seroreactive to JCV, and viral DNA can be detected in up to 50% of normal kidney tissue...
  27. HIV integrase/DNA complexes and concerted integration
    Duane P Grandgenett; Fiscal Year: 2010
    ..The viral integrase (IN) within the PIC cleaves a dinucleotide from the 3'OH ends of the viral DNA prior to nuclear transport...
  28. Stage-Specific Inhibitors of Orthopoxviruses
    John H Connor; Fiscal Year: 2012
    ..to develop and optimize the most interesting of our identified compounds (likely those that inhibit after viral DNA replication) towards high-efficacy low-toxicity antipoxviral compounds...
  29. Understanding the early and late endosomal TLR9-mediated responses to viral DNA.
    Yong Jun Liu; Fiscal Year: 2013
    ..The third aim will focus on further characterization of three potential PACSIN1-interacting molecules including CD2AP, MEKK4 and TRAF4. ..
  30. Chromatin and Coactivators in HSV-1 gene regulation
    STEVEN TRIEZENBERG; Fiscal Year: 2009
    ..However, previous reports indicated that the large HSV-1 viral DNA genome remains predominantly non-nucleosomal during lytic infection...
  31. Targeting_the_Source_of_Persistent_HIV_Viremia
    Sarah Palmer; Fiscal Year: 2010
    ..The phylogenetic relatedness of the plasma-derived viral RNA sequences and intracellular viral DNA sequences will be determined...
  32. Novel Fluorescent Nucleotide Analogues to Probe Nucleic Acid Metabolism
    BYRON WILLIS PURSE; Fiscal Year: 2010
    ..PUBLIC HEALTH RELEVANCE: DNA replication plays multiple critical roles in health care and disease - cellular and viral DNA replication are primary targets for cancer chemotherapeutics and antiviral agents, and incorrect replication can ..
  33. PHAGE T4 HEAD ASSEMBLY AND INITIATION OF INFECTION
    Lindsay W Black; Fiscal Year: 2013
    ..of research toward two long term objectives: understanding 1) the translocation mechanism of the conserved viral DNA packaging motor and its regulation;and 2) the evolution of the internal proteins injected to counter novel DNA ..
  34. Vaccinia Virus Genetics and Morphogenesis
    Richard C Condit; Fiscal Year: 2013
    ..The core contains the viral DNA and at least 50 viral proteins...
  35. Development of the Gag/Lysyl tRNA synthetase interaction as a target for anti-HIV
    Lawrence Kleiman; Fiscal Year: 2012
    ..by applicant): The genome of HIV-1 is composed of RNA, and after this RNA enters a cell, it must be converted to viral DNA, which can then insert into the cell's DNA, and be used to produce new viral protein and viruses...
  36. Chronic CMV infection in the elderly: diagnosis and link to chronic inflammation
    Sean X Leng; Fiscal Year: 2013
    ..This application seeks to characterize CMV viral DNA in monocytes detected by a nested PCR-based assay and test the hypothesis that CMV DNA in monocytes predicts T-..
  37. Encapsidation of Adenovirus DNA
    Michael J Imperiale; Fiscal Year: 2010
    ..This project addresses assembly with a focus on the encapsidation of viral DNA. This process requires at least four viral elements: the packaging sequence, which is located near the left end ..
  38. Regulation of DNA Metabolism During Herpes Simplex Virus-1 Infection
    KEVIN FRANCIS BRYANT; Fiscal Year: 2010
    ..are recruited to viral replication compartments in the nuclei of infected host cells, which are the sites of viral DNA replication and late gene transcription...
  39. Retinoblastoma (Rb) protein pathway in human cytomegalovirus infected cells
    Robert F Kalejta; Fiscal Year: 2013
    ..that some of these E2F-responsive gene products (the NBEs) are responsible for the UL97-mediated stimulation of viral DNA replication...
  40. Designer Nucleases to Cure Chronic Hepatitis B
    Christoph Seeger; Fiscal Year: 2013
    ..effector nucleases (TALENs) specific for CCC DNA to cleave and mutate CCC DNA in regions essential for viral DNA replication...
  41. Functional Dynamics of Mammalian and Viral DNA Repair Polymerases
    W M BUJALOWSKI; Fiscal Year: 2013
    ....
  42. Viral Mediated Type I Interferon Induction
    Genhong Cheng; Fiscal Year: 2013
    ..sensors that can detect viral RNA sequences;however, the receptors responsible for recognizing intracellular viral DNA still remain to be determined...
  43. Molecular Mechanism of Hepadnavirus Persistence
    Jianming Hu; Fiscal Year: 2012
    ..Current antiviral therapy for chronic hepatitis B is only partially effective. In particular, the episomal viral DNA, the so-called covalently closed circular (CCC) DNA, persists in the infected cell nucleus even after years of ..
  44. Mechanisms in Polyoma and Papillomavirus Assembly
    ROBERT GARCEA; Fiscal Year: 2013
    ..marked by a nonstructural viral protein such as the polyoma large T-antigen or papilloma E2 protein bound to the viral DNA. By unknown mechanisms this encounter triggers the assembly of the capsid proteins around the viral ..
  45. Immunological response, Viral Factors and Helminth Infections in Disease Expressi
    Edgar M Carvalho; Fiscal Year: 2012
    ..Proviral load will be measured and viral DNA will be stored for future studies aimed at evaluating viral polymorphisms that could be associated with HAM/TSP...
  46. Mechanisms of Viral DNA Packaging: Biophysical, Biochemical, &Genetic Analysis
    Douglas E Smith; Fiscal Year: 2013
    ..abstract_text> ..
  47. DISSECTION OF THE FUNCTIONS OF HERPES SIMPLEX VIRUS ICPO
    Bernard Roizman; Fiscal Year: 2012
    ..In essence, on release of viral DNA from virions into the nucleus, it is confronted by numerous cellular proteins whose function it is to either ..
  48. Novel SIV Model of HAND
    Joseph L Mankowski; Fiscal Year: 2013
    ..with non-CNS-penetrant ART (ncART) for ability to 1) control viral replication&inflammation 2) reduce viral DNA reservoirs in brain 3) preserve markers of neuronal function...
  49. Understanding the mechanism of KSHV latent DNA replication
    Subhash C Verma; Fiscal Year: 2011
    ..These latent genes have been shown to induce cell proliferation/tumorigenesis. Viral DNA which persists as an episome gets passage to the dividing tumor cells during tumorigenesis...
  50. KSHV-Ori-Lyt-Dependent DNA Replication
    Yan Yuan; Fiscal Year: 2012
    ..e. formation of viral replisomes and their recruitment onto ori-Lyt, involvement of cellular factors in viral DNA replication and regulation of viral DNA replication by epigenetic effects...
  51. Human Stem Cell Recombineering
    MELVYS VALLEDOR CEBALLOS; Fiscal Year: 2012
    ..For instance, the viral DNA binding protein protects the ssDNA recombination substrate from degradation by host nucleases...
  52. INTERACTIONS OF HPV ONCOGENES WITH THE P53 PATHWAY
    Denise A Galloway; Fiscal Year: 2013
    ..Using both proliferative and differentiation-promoting growth conditions we will measure episome maintenance, viral DNA amplification, late gene expression, and epithelial differentiation. 4...
  53. EARLY INTERACTIONS OF DNA VIRUSES AND HOST
    Gerd Maul; Fiscal Year: 2009
    ..Current HCMV interference strategies targeting early events in viral DNA replication are neither permissible nor feasible for treating the infected fetus, and may lead to drug ..
  54. Improving screening tools to better predict high-grade anal dysplasia for MSM
    Dorothy J Wiley; Fiscal Year: 2013
    ..Two molecular HPV tests that detect viral DNA &-mRNA may be relevant for IAC screening: HPV-Hybrid-capture II (HC-2) &-APTIMA...
  55. Identification of novel inhibitors targeting entry of human cytomegalovirus
    Domenico Tortorella; Fiscal Year: 2013
    ..Current therapy against HCMV targets the viral DNA polymerase, but induces severe side effects (e.g...
  56. VACCINIA DNA REPLICATION
    Paula Traktman; Fiscal Year: 2013
    ..We will also investigate the roles of the viral DNA ligase and the G5 protein, which has homology to FEN1 nucleases, in the replication and maturation of the viral ..
  57. Development of a Primate Model for Genital Papillomavirus Infection
    Robert D Burk; Fiscal Year: 2010
    ..This was demonstrated by identifying viral DNA and RNA in recipient animals...
  58. Determinants of HIV Dynamics and Variation
    JOSE ORLANDO MALDONADO-ORTIZ; Fiscal Year: 2013
    ..fitness compared to wt virus using dual competition assay and b) the role of A62V on the efficiency of HIV-1 viral DNA synthesis by real-time PCR...
  59. DNA sensors and associated signaling pathways in the innate immune response
    Katherine A Fitzgerald; Fiscal Year: 2013
    ..Knockdown of IFI16, or its murine ortholog p204 prevents viral DNA from triggering IRF3 activation and IFN? gene induction, while not affecting responses to cytosolic RNA...
  60. Genome packaging in DNA viruses
    Liang Tang; Fiscal Year: 2013
    ..Genome packaging is a precisely coordinated molecular synergy, in which a certain amount of viral DNA from a DNA concatemer is inserted into a preformed procapsid, followed by binding of additional viral proteins ..
  61. Primate Model Towards HIV Eradication Strategies
    RAYMOND FELIX SCHINAZI; Fiscal Year: 2012
    ..viral RNA (vRNA) per ml of plasma, and have also developed sensitive RT-PCR and PCR assays to measure vRNA and viral DNA (vDNA) in tissues...
  62. REPRESSION AND ACTIVATION OF PERSISTING HSV GENOMES
    Neal A DeLuca; Fiscal Year: 2013
    ..Nucleoside analogues, such as acyclovir and its derivatives, target viral DNA replication. However, nucleoside analogs have little effect on the latent state...
  63. MECHANISTIC STUDIES ON VIRAL ASSEMBLY
    CARLOS CATALANO; Fiscal Year: 1999
    ..lambda has been extensively studied over the years and represents an ideal system in which to study viral DNA packaging...
  64. Adenovirus Persistence in Human Lymphoid Tissues
    Linda Gooding; Fiscal Year: 2007
    ..Preliminary experiments suggest that viral DNA is located within T cells and a second cell type, perhaps a phagocyte...
  65. METAL CARCINOGEN-INDUCED MUTATIONS IN MAMMALIAN GENES
    EDWIN MURPHY; Fiscal Year: 1992
    ..In the chromium- and cadmium-induced revertants, apparent frameshift mutations in the MuSVts110 viral DNA allow the expression of a gag-mos fusion protein from the unspliced viral RNA...
  66. Mechanistic Studies on a Viral DNA Packaging Machine
    CARLOS CATALANO; Fiscal Year: 2009
    ..Terminase enzymes are common to these viruses and function to package viral DNA into the capsid. Common mechanisms for genome packaging have been proposed for these all viruses...
  67. PF-8 AND POL-8 OF KSHV AS TARGETS OF VIRAL DNA SYNTHESIS
    Robert Ricciardi; Fiscal Year: 2001
    ..The PF genes of certain other herpesviruses are known to be required for viral DNA synthesis and infection...
  68. Cytidine deaminase and HIV-1 replication
    Hui Zhang; Fiscal Year: 2007
    ..HIV-1) replication, is a cytidine deaminase and is able to induce G to A hypermutation in the newly-synthesized viral DNA. This effect can be counteracted by the HIV- 1 virion infectivity factor (Vif)...
  69. Varicella-Zoster Virus Encapsidation Proteins
    Robert J Visalli; Fiscal Year: 2010
    ..Proteins that play a role in the herpesviral DNA encapsidation process (the cleaving and packaging of viral DNA into capsids) have become promising novel antiviral targets...
  70. Novel Small Molecule Inhibitors of HIV
    Xiao Fang Yu; Fiscal Year: 2010
    ..These antiviral proteins induce lethal modification of cytosines to uracils in newly synthesized minus-strand viral DNA, resulting in the production of non-functional viruses in the absence of the retroviral Vif protein...
  71. STRUCTURE AND FUNCTION OF THE HSV DNA POLYMERASE
    DAVID DORSKY; Fiscal Year: 1993
    ..Recently, several inhibitors of viral DNA polymerases have emerged as clinically useful drugs for the treatment of some herpesvirus infections...