complementary dna

Summary

Summary: Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.

Top Publications

  1. pmc Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
    Jo Vandesompele
    Center for Medical Genetics, Ghent University Hospital 1K5, De Pintelaan 185, B 9000 Ghent, Belgium
    Genome Biol 3:RESEARCH0034. 2002
  2. pmc The transcriptional landscape of the yeast genome defined by RNA sequencing
    Ugrappa Nagalakshmi
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    Science 320:1344-9. 2008
  3. ncbi Normalization of cDNA microarray data
    Gordon K Smyth
    Walter and Eliza Hall Institute, 1G Royal Parade, Parkville, VIC 3050, Australia
    Methods 31:265-73. 2003
  4. ncbi Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data
    Christian Ramakers
    Department of Anatomy and Embryology K2 283, Experimental and Molecular Cardiology Group, Academic Medical Centre, University of Amsterdam, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands
    Neurosci Lett 339:62-6. 2003
  5. pmc Transcriptome analysis by strand-specific sequencing of complementary DNA
    Dmitri Parkhomchuk
    Max Planck Institute for Molecular Genetics, Department of Vertebrate Genomics, Ihnestr 73, 14195 Berlin, Germany
    Nucleic Acids Res 37:e123. 2009
  6. ncbi A global view of gene activity and alternative splicing by deep sequencing of the human transcriptome
    Marc Sultan
    Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Ihnestrasse 73, 14195 Berlin, Germany
    Science 321:956-60. 2008
  7. pmc Simple cDNA normalization using kamchatka crab duplex-specific nuclease
    Pavel A Zhulidov
    Shemiakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, Miklukho Maklaya 16 10, 117871 Moscow, Russia
    Nucleic Acids Res 32:e37. 2004
  8. ncbi The transcriptional landscape of the mammalian genome
    P Carninci
    Science 309:1559-63. 2005
  9. pmc Occludin: a novel integral membrane protein localizing at tight junctions
    M Furuse
    Department of Information Physiology, National Institute for Physiological Sciences, Aichi, Japan
    J Cell Biol 123:1777-88. 1993
  10. ncbi A small ubiquitin-related polypeptide involved in targeting RanGAP1 to nuclear pore complex protein RanBP2
    R Mahajan
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Cell 88:97-107. 1997

Research Grants

  1. True Direct Single Molecule RNA Sequencing
    PATRICE MARIE MILOS; Fiscal Year: 2010
  2. INTESTINAL AND COLONIC CYTOCHROME P450 GENE EXPRESSION
    Peter Traber; Fiscal Year: 1990
  3. ANDROGEN RECEPTOR REGULATION OF REPRODUCTIVE FUNCTION
    Elizabeth Wilson; Fiscal Year: 1990
  4. ANDROGEN RECEPTOR REGULATION OF REPRODUCTIVE FUNCTION
    Elizabeth Wilson; Fiscal Year: 1991
  5. ANDROGEN RECEPTOR REGULATION OF REPRODUCTIVE FUNCTION
    Elizabeth Wilson; Fiscal Year: 1992
  6. Immunoglobulin Somatic Mutation
    Ursula Storb; Fiscal Year: 2007
  7. MUTATION OF SKELETAL TROPONIN C FUNCTIONAL DOMAINS
    James Potter; Fiscal Year: 1991
  8. EFFECTS OF SENSORY DEPRIVATION ON ADULT VISUAL CORTEX
    Edward Jones; Fiscal Year: 1999
  9. DEVELOPMENT OF DNA PROBES TO PERIODONTAL PATHOGENS
    BRUCE PASTER; Fiscal Year: 1990
  10. RENIN-ANGIOTENSIN AND GROWTH IN URETERAL OBSTRUCTION
    ROBERT CHEVALIER; Fiscal Year: 1990

Detail Information

Publications243 found, 100 shown here

  1. pmc Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
    Jo Vandesompele
    Center for Medical Genetics, Ghent University Hospital 1K5, De Pintelaan 185, B 9000 Ghent, Belgium
    Genome Biol 3:RESEARCH0034. 2002
    ....
  2. pmc The transcriptional landscape of the yeast genome defined by RNA sequencing
    Ugrappa Nagalakshmi
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    Science 320:1344-9. 2008
    ..We developed a quantitative sequencing-based method called RNA-Seq for mapping transcribed regions, in which complementary DNA fragments are subjected to high-throughput sequencing and mapped to the genome...
  3. ncbi Normalization of cDNA microarray data
    Gordon K Smyth
    Walter and Eliza Hall Institute, 1G Royal Parade, Parkville, VIC 3050, Australia
    Methods 31:265-73. 2003
    ..Variations on loess normalization include global loess normalization and two-dimensional normalization. Detailed commands are given to implement the normalization techniques using freely available software...
  4. ncbi Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data
    Christian Ramakers
    Department of Anatomy and Embryology K2 283, Experimental and Molecular Cardiology Group, Academic Medical Centre, University of Amsterdam, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands
    Neurosci Lett 339:62-6. 2003
    ..A computer program to perform this calculation is available on request (e-mail: bioinfo@amc.uva.nl; subject: LinRegPCR)...
  5. pmc Transcriptome analysis by strand-specific sequencing of complementary DNA
    Dmitri Parkhomchuk
    Max Planck Institute for Molecular Genetics, Department of Vertebrate Genomics, Ihnestr 73, 14195 Berlin, Germany
    Nucleic Acids Res 37:e123. 2009
    High-throughput complementary DNA sequencing (RNA-Seq) is a powerful tool for whole-transcriptome analysis, supplying information about a transcript's expression level and structure...
  6. ncbi A global view of gene activity and alternative splicing by deep sequencing of the human transcriptome
    Marc Sultan
    Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Ihnestrasse 73, 14195 Berlin, Germany
    Science 321:956-60. 2008
    ..A global survey of messenger RNA splicing events identified 94,241 splice junctions (4096 of which were previously unidentified) and showed that exon skipping is the most prevalent form of alternative splicing...
  7. pmc Simple cDNA normalization using kamchatka crab duplex-specific nuclease
    Pavel A Zhulidov
    Shemiakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, Miklukho Maklaya 16 10, 117871 Moscow, Russia
    Nucleic Acids Res 32:e37. 2004
    ..We also employed DSN normalization to normalize cDNA from nervous tissues of the marine mollusc Aplysia californica (a popular model organism in neuroscience) to illustrate further the efficiency of the normalization technique...
  8. ncbi The transcriptional landscape of the mammalian genome
    P Carninci
    Science 309:1559-63. 2005
    ..The data provide a comprehensive platform for the comparative analysis of mammalian transcriptional regulation in differentiation and development...
  9. pmc Occludin: a novel integral membrane protein localizing at tight junctions
    M Furuse
    Department of Information Physiology, National Institute for Physiological Sciences, Aichi, Japan
    J Cell Biol 123:1777-88. 1993
    ..These findings revealed that an integral membrane protein localizing at tight junctions is now identified, which we designated as "occludin."..
  10. ncbi A small ubiquitin-related polypeptide involved in targeting RanGAP1 to nuclear pore complex protein RanBP2
    R Mahajan
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Cell 88:97-107. 1997
    ..Inhibition of nuclear protein import resulting from antibodies directed at NPC-associated RanGAP1 cannot be overcome by soluble cytosolic RanGAP1, indicating that GTP hydrolysis by Ran at RanBP2 is required for nuclear protein import...
  11. pmc The cotton ACTIN1 gene is functionally expressed in fibers and participates in fiber elongation
    Xue Bao Li
    College of Life Sciences, Central China Normal University, Wuhan 430079, China
    Plant Cell 17:859-75. 2005
    ..No defined actin network was observed in these fibers and, consequently, fiber elongation was inhibited. Our results suggested that GhACT1 plays an important role in fiber elongation but not fiber initiation...
  12. pmc Insights into corn genes derived from large-scale cDNA sequencing
    Nickolai N Alexandrov
    Ceres, Inc, 1535 Rancho Conejo Blvd, Thousand Oaks, CA 91320, USA
    Plant Mol Biol 69:179-94. 2009
    ..All of the sequence data from this study have been submitted to DDBJ/GenBank/EMBL under accession numbers EU940701-EU977132 (FLI cDNA) and FK944382-FL482108 (EST)...
  13. pmc Automated generation of heuristics for biological sequence comparison
    Guy St C Slater
    The Ensembl Group, EMBL European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SD, UK
    BMC Bioinformatics 6:31. 2005
    ....
  14. doi mRNA-Seq whole-transcriptome analysis of a single cell
    Fuchou Tang
    Wellcome Trust Cancer Research UK Gurdon Institute of Cancer and Developmental Biology, University of Cambridge, Cambridge, UK
    Nat Methods 6:377-82. 2009
    ..Finally, for Dicer1(-/-) and Ago2(-/-) (Eif2c2(-/-)) oocytes, we found that 1,696 and 1,553 genes, respectively, were abnormally upregulated compared to wild-type controls, with 619 genes in common...
  15. ncbi Real-time RT-PCR normalisation; strategies and considerations
    J Huggett
    Centre for Infectious Diseases and International Health, University College London, London, UK
    Genes Immun 6:279-84. 2005
    ..In this review we discuss the relative merits of different normalisation strategies and suggest a method of validation that will enable the measurement of biologically meaningful results...
  16. pmc The TIGR Rice Genome Annotation Resource: improvements and new features
    Shu Ouyang
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Nucleic Acids Res 35:D883-7. 2007
    ..Enhanced data access is available through web interfaces, FTP downloads and a Data Extractor tool developed in order to support discrete dataset downloads...
  17. ncbi Detoxification of the Fusarium mycotoxin deoxynivalenol by a UDP-glucosyltransferase from Arabidopsis thaliana
    Brigitte Poppenberger
    Center of Applied Genetics, BOKU University of Natural Resources and Applied Life Sciences, Muthgasse 18, A 1190 Vienna, Austria
    J Biol Chem 278:47905-14. 2003
    ..Constitutive overexpression in Arabidopsis leads to enhanced tolerance against deoxynivalenol...
  18. ncbi DNA deamination mediates innate immunity to retroviral infection
    Reuben S Harris
    Medical Research Council Laboratory of Molecular Biology, Hills Road, CB2 2QH, Cambridge, United Kingdom
    Cell 113:803-9. 2003
    ..These findings imply that targeted DNA deamination is a major strategy of innate immunity to retroviruses and likely also contributes to the sequence variation observed in many viruses (including HIV)...
  19. pmc Transferability of the EST-SSRs developed on Nules clementine (Citrus clementina Hort ex Tan) to other Citrus species and their effectiveness for genetic mapping
    François L Luro
    INRA, Unité de Recherche GEQA, INRA San Giuliano, 20230 San Nicolao, France
    BMC Genomics 9:287. 2008
    ....
  20. pmc The lateral organ boundaries gene defines a novel, plant-specific gene family
    Bin Shuai
    Department of Botany and Plant Sciences, University of California, Riverside, CA 92521 0124, USA
    Plant Physiol 129:747-61. 2002
    ..The expression of LOB at the base of lateral organs suggests a potential role for LOB in lateral organ development...
  21. pmc AceView: a comprehensive cDNA-supported gene and transcripts annotation
    Danielle Thierry-Mieg
    National Center for Biotechnology Information, National Library of Medicine, NIH, Bethesda, MD 20894, USA
    Genome Biol 7:S12.1-14. 2006
    ..The organizers evaluated the protein predictions in depth. We present a complementary analysis of the mRNAs, including alternative transcript variants...
  22. pmc Calcium-dependent protein kinases play an essential role in a plant defence response
    T Romeis
    The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, Norfolk, UK
    EMBO J 20:5556-67. 2001
    ..Silencing correlated with loss of CDPK mRNA, whereas mRNA accumulation of mitogen-activated protein kinase WIPK remained unaltered...
  23. ncbi Transmembrane phosphoprotein Cbp regulates the activities of Src-family tyrosine kinases
    M Kawabuchi
    Division of Protein Metabolism, Institute for Protein Research, Osaka University, Suita, Japan
    Nature 404:999-1003. 2000
    ..These findings reveal Cbp as a new component of the regulatory mechanism controlling the activity of membrane-associated Src-PTKs...
  24. doi Genome-wide identification of human RNA editing sites by parallel DNA capturing and sequencing
    Jin Billy Li
    Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Science 324:1210-3. 2009
    ..This efficient approach greatly expands the repertoire of RNA editing targets and can be applied to studies involving RNA editing-related human diseases...
  25. doi Profiling the HeLa S3 transcriptome using randomly primed cDNA and massively parallel short-read sequencing
    Ryan Morin
    Genome Sciences Centre, BC Cancer Agency, Vancouver, British Columbia, Canada
    Biotechniques 45:81-94. 2008
    ..Analysis of these variants, and previously unannotated splicing events in the HeLa S3 cell line, reveals an overrepresentation of gene categories including those previously implicated in cancer...
  26. ncbi Molecular cloning of a novel laminin chain, alpha 5, and widespread expression in adult mouse tissues
    J H Miner
    Department of Anatomy and Neurobiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 270:28523-6. 1995
    ..Analysis of RNA expression showed that alpha 5 is widely expressed in adult tissues, with highest levels in lung, heart, and kidney. Our results suggest that alpha 5 may be a major laminin chain of adult basal laminae...
  27. pmc Gene discovery and annotation using LCM-454 transcriptome sequencing
    Scott J Emrich
    Bioinformatics and Computational Biology Graduate Program, Iowa State University, Ames, IA 50010, USA
    Genome Res 17:69-73. 2007
    ..We conclude that the coupling of LCM and 454 sequencing technologies facilitates the discovery of rare, possibly cell-type-specific transcripts...
  28. pmc Phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), a novel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase csk and is involved in regulation of T cell activation
    T Brdicka
    Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, 14220 Prague, Czech Republic
    J Exp Med 191:1591-604. 2000
    ..These findings collectively suggest that in the absence of external stimuli, the PAG-Csk complex transmits negative regulatory signals and thus may help to keep resting T cells in a quiescent state...
  29. ncbi Osf2/Cbfa1: a transcriptional activator of osteoblast differentiation
    P Ducy
    Department of Molecular Genetics, M D Anderson Cancer Center, University of Texas, Houston 77030, USA
    Cell 89:747-54. 1997
    ..This study identifies Osf2/Cbfa1 as an osteoblast-specific transcription factor and as a regulator of osteoblast differentiation...
  30. pmc Clustering cancer gene expression data: a comparative study
    Marcilio C P De Souto
    Computational Molecular Biology, Max Planck Institute for Molecular Genetics, Berlin, Germany
    BMC Bioinformatics 9:497. 2008
    ..There have been no studies thus far performing a large-scale evaluation of different clustering methods in this context...
  31. ncbi A new class of membrane-bound chemokine with a CX3C motif
    J F Bazan
    Department of Molecular Biology, DNAX Research Institute, Palo Alto, California 94304, USA
    Nature 385:640-4. 1997
    ....
  32. pmc Envoplakin, a novel precursor of the cornified envelope that has homology to desmoplakin
    C Ruhrberg
    Keratinocyte Laboratory, Imperial Cancer Research Fund, London, United Kingdom
    J Cell Biol 134:715-29. 1996
    ..These data suggest that the 210-kD protein may link the cornified envelope to desmosomes and keratin filaments. We propose that the 210-kD protein be named "envoplakin."..
  33. ncbi Mutations in the DJ-1 gene associated with autosomal recessive early-onset parkinsonism
    Vincenzo Bonifati
    Genetic Epidemiologic Unit, Department of Clinical Genetics, Department of Epidemiology and Biostatistics, Erasmus Medical Center Rotterdam, Post Office Box 1738, 3000 DR Rotterdam, Netherlands
    Science 299:256-9. 2003
    ..Elucidating the physiological role of DJ-1 protein may promote understanding of the mechanisms of brain neuronal maintenance and pathogenesis of Parkinson's disease...
  34. pmc Polyphenol oxidase from hybrid poplar. Cloning and expression in response to wounding and herbivory
    C P Constabel
    Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada TGG 2E9
    Plant Physiol 124:285-95. 2000
    ..This wound- and herbivore-induced expression of PPO in hybrid poplar supports the defensive role of this protein against insect pests...
  35. ncbi A novel jasmonic acid-inducible rice myb gene associates with fungal infection and host cell death
    M W Lee
    Department of Plant Pathology, University of Arkansas, Fayetteville 72701, USA
    Mol Plant Microbe Interact 14:527-35. 2001
    ..These results suggest that JAmyb is related closely to host cell death and is involved in the JA-mediated, SA-independent signaling pathways in rice...
  36. pmc Inhibition of vascular endothelial cell growth factor activity by an endogenously encoded soluble receptor
    R L Kendall
    Department of Biochemistry, Merck Research Laboratories, Rahway, NJ 07065
    Proc Natl Acad Sci U S A 90:10705-9. 1993
    ....
  37. pmc MYB98 is required for pollen tube guidance and synergid cell differentiation in Arabidopsis
    Ryushiro D Kasahara
    Department of Biology, University of Utah, Salt Lake City, Utah 84112 0840, USA
    Plant Cell 17:2981-92. 2005
    ..Together, these data suggest that MYB98 controls the development of specific features within the synergid cell during female gametophyte development...
  38. ncbi Isolation and characterization of UGT2B15(Y85): a UDP-glucuronosyltransferase encoded by a polymorphic gene
    E Levesque
    Medical Research Council Group in Molecular Endocrinology, CHUL Research Center, Laval University, Quebec, Canada
    Pharmacogenetics 7:317-25. 1997
    ..The wide expression of UGT2B15 in many tissues indicates that it is a major glucuronidation enzyme in humans...
  39. pmc Analysis of small human proteins reveals the translation of upstream open reading frames of mRNAs
    Masaaki Oyama
    Human Genome Center, Institute of Medical Science, University of Tokyo, Minato ku, Tokyo 108 8639, Japan
    Genome Res 14:2048-52. 2004
    ..This investigation provides the first direct evidence of translation of upstream ORFs in human cells, which could greatly change the current outline of the human proteome...
  40. ncbi Large-scale statistical analysis of secondary xylem ESTs in pine
    Nathalie Pavy
    ARBOREA and Centre de Recherche en Biologie Forestiére, Universite Laval, Pavillon Charles Eugène Marchand, Sainte Foy, Que, G1K 7P4, Canada
    Plant Mol Biol 57:203-24. 2005
    ..Thus, we illustrated the potential of extracting information from conifer sequences already accessible through well-structured public databases...
  41. ncbi Migfilin and Mig-2 link focal adhesions to filamin and the actin cytoskeleton and function in cell shape modulation
    Yizeng Tu
    Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Cell 113:37-47. 2003
    ..Together, Mig-2, migfilin and filamin define a connection between cell matrix adhesions and the actin cytoskeleton and participate in the orchestration of actin assembly and cell shape modulation...
  42. ncbi Discovery of the genes in response to white spot syndrome virus (WSSV) infection in Fenneropenaeus chinensis through cDNA microarray
    Bing Wang
    Institute of Oceanology, Chinese Academy of Sciences, Qingdao, People s Republic of China
    Mar Biotechnol (NY) 8:491-500. 2006
    ..As a substitute, we found that triosephosphate isomerase (TPI) was an ideal candidate of interstandards in this situation...
  43. ncbi The FHIT gene, spanning the chromosome 3p14.2 fragile site and renal carcinoma-associated t(3;8) breakpoint, is abnormal in digestive tract cancers
    M Ohta
    Kimmel Cancer Center, Jefferson Medical College, Philadelphia, Pennsylvania, USA
    Cell 84:587-97. 1996
    ..Aberrant transcripts of the FHIT locus were found in approximately 50% of esophageal, stomach, and colon carcinomas...
  44. pmc A widely expressed betaIII spectrin associated with Golgi and cytoplasmic vesicles
    M C Stankewich
    Department of Pathology, Yale University, New Haven, CT 06520, USA
    Proc Natl Acad Sci U S A 95:14158-63. 1998
    ..Liver Golgi membranes and other vesicular compartment markers cosediment in vitro with betaIII spectrin. betaIII spectrin thus constitutes a major component of the Golgi and vesicular membrane skeletons...
  45. pmc Mapping the Burkholderia cenocepacia niche response via high-throughput sequencing
    D R Yoder-Himes
    Center for Microbial Ecology, Michigan State University, East Lansing, MI 48824, USA
    Proc Natl Acad Sci U S A 106:3976-81. 2009
    ..Compared with the CF strain, the soil strain shows a stronger global gene expression response to its environment, which is consistent with the need for a more dynamic reaction to the heterogeneous conditions of soil...
  46. ncbi SINEs and LINEs share common 3' sequences: a review
    N Okada
    Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan
    Gene 205:229-43. 1997
    ..This organization of SINEs and LINEs provides the basis for a simple general scheme by which SINEs might acquire retropositional activity...
  47. ncbi Overexpression of the cell adhesion molecules DDR1, Claudin 3, and Ep-CAM in metaplastic ovarian epithelium and ovarian cancer
    Viola A Heinzelmann-Schwarz
    Cancer Research Program, Garvan Institute of Medical Research, Darlinghurst, Australia
    Clin Cancer Res 10:4427-36. 2004
    ..The aims of this study were to integrate the results from 14 transcript profiling studies of EOC to identify novel biomarkers and to examine their expression in early and late stages of the disease...
  48. ncbi Gene expression analysis of human thymoma correlates with tumor stage
    Hidefumi Sasaki
    Department of Surgery II, Nagoya City University Medical School, Nagoya, Japan
    Int J Cancer 101:342-7. 2002
    ..type B1, B2 or AB). The combined use of oligonucleotide microarray and real-time RT-PCR analyses provides a powerful new approach to elucidate the in vivo molecular events correlated with tumor stage of thymoma...
  49. ncbi Overexpression of spermidine synthase enhances tolerance to multiple environmental stresses and up-regulates the expression of various stress-regulated genes in transgenic Arabidopsis thaliana
    Yoshihisa Kasukabe
    Toyobo Research Center Co Ltd, Katata, Ohtsu, Shiga, 520 0292 Japan
    Plant Cell Physiol 45:712-22. 2004
    ..These results strongly suggest an important role for spermidine as a signaling regulator in stress signaling pathways, leading to build-up of stress tolerance mechanisms in plants under stress conditions...
  50. ncbi Natural killer cell receptors in the horse: evidence for the existence of multiple transcribed LY49 genes
    Tomoko Takahashi
    Department of Biosystems Science, School of Advanced Sciences, The Graduate University for Advanced Studies Sokendai, Hayama, Japan
    Eur J Immunol 34:773-84. 2004
    ..We also show that horse spleen expresses ILT-like genes with unique domain organizations. Radiation hybrid mapping and fluorescence in situ hybridization localized horse LY49 and KIR/ILT genes to chromosomes 6q13 and 10p12, respectively...
  51. ncbi An excitatory amino-acid transporter with properties of a ligand-gated chloride channel
    W A Fairman
    Howard Hughes Medical Institute, Oregon Health Sciences University, Portland 97201, USA
    Nature 375:599-603. 1995
    ..Thus EAAT4 combines the re-uptake of neurotransmitter with a mechanism for increasing chloride permeability, both of which could regulate excitatory neurotransmission...
  52. ncbi Characterization of a notochord-specific enhancer from the Brachyury promoter region of the ascidian, Ciona intestinalis
    J C Corbo
    Department of Biology, University of California, San Diego, La Jolla 92093 0347, USA
    Development 124:589-602. 1997
    ..We discuss the implications of this analysis with regard to the evolutionary conservation of integrative enhancers, and the subdivision of the axial and paraxial mesoderm in vertebrates...
  53. ncbi Two zebrafish eIF4E family members are differentially expressed and functionally divergent
    Javier Robalino
    Center of Marine Biotechnology, Baltimore, Maryland 21202, USA
    J Biol Chem 279:10532-41. 2004
    ....
  54. pmc Molecular cloning of NKp46: a novel member of the immunoglobulin superfamily involved in triggering of natural cytotoxicity
    A Pessino
    Dipartimento di Medicina Sperimentale, Universita degli Studi di Genova, Italy
    J Exp Med 188:953-60. 1998
    ..In conclusion, this study reports the first characterization of the molecular structure of a NK-specific receptor involved in the mechanism of NK cell activation during natural cytotoxicity...
  55. pmc BNaC1 and BNaC2 constitute a new family of human neuronal sodium channels related to degenerins and epithelial sodium channels
    J Garcia-Anoveros
    Department of Neurobiology, Massachusetts General Hospital, Harvard Medical School, Boston 02114, USA
    Proc Natl Acad Sci U S A 94:1459-64. 1997
    ..By analogy to the ENaCs and the degenerins, which form heteromultimeric channels, BNaC1 and BNaC2 may be subunits of the same channel...
  56. ncbi Isolation and characterization of the faciogenital dysplasia (Aarskog-Scott syndrome) gene: a putative Rho/Rac guanine nucleotide exchange factor
    N G Pasteris
    Department of Human Genetics, University of Michigan, Ann Arbor 48109 0688
    Cell 79:669-78. 1994
    ..These results provide compelling evidence that FGD1 is responsible for FGDY and suggest that FGD1 is a Rho/RacGEF involved in mammalian development...
  57. ncbi Grap is a novel SH3-SH2-SH3 adaptor protein that couples tyrosine kinases to the Ras pathway
    G S Feng
    Department of Biochemistry and Molecular Biology, Walther Oncology Center, Indiana University School of Medicine, Indianapolis 46202 5121, USA IUPUI EDU
    J Biol Chem 271:12129-32. 1996
    ..These results show that a family of Grb2-like proteins exist and couple signals from receptor and cytoplasmic tyrosine kinases to the Ras signaling pathway...
  58. ncbi Differential gene expression profiling of adult murine hematopoietic stem cells
    In Kyung Park
    University of Michigan, Department of Internal Medicine, Ann Arbor, USA
    Blood 99:488-98. 2002
    ..A large number of genes that were differentially expressed by enriched populations of HSCs and MPP cells were identified. These included transcription factors, signaling molecules, and previously unknown genes...
  59. ncbi B7h, a novel costimulatory homolog of B7.1 and B7.2, is induced by TNFalpha
    M M Swallow
    Molecular and Cell Biology Department, University of California, Berkeley 94720, USA
    Immunity 11:423-32. 1999
    ..These data define a novel costimulatory ligand for T cells and suggest that induction of B7h by TNFalpha may function as a mechanism to directly augment recognition of self during inflammation...
  60. pmc Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling
    Hiroshi Abe
    Biological Resources Division, Japan International Research Center for Agricultural Sciences, 1 1 Ohwashi, Tsukuba, Ibaraki 305 8686, Japan
    Plant Cell 15:63-78. 2003
    ..These results indicate that both AtMYC2 and AtMYB2 proteins function as transcriptional activators in ABA-inducible gene expression under drought stress in plants...
  61. pmc Cloning of mammalian Ire1 reveals diversity in the ER stress responses
    X Z Wang
    Skirball Institute of Biomolecular Medicine, the Departments of Medicine, Cell Biology and the Kaplan Cancer Center, NYU Medical Center, New York, NY 10016, USA
    EMBO J 17:5708-17. 1998
    ..These results indicate that a single upstream component, Ire1, plays a role in multiple facets of the ER stress-response in mammalian cells...
  62. pmc Development of 5006 full-length CDNAs in barley: a tool for accessing cereal genomics resources
    Kazuhiro Sato
    Research Institute for Bioresources, Okayama University, Kurashiki, Japan
    DNA Res 16:81-9. 2009
    ..Sequence data from this article have been deposited with the DDBJ/EMBL/GenBank Data Libraries under accession nos AK248134-AK253139. The online database with annotation is available at http://www.shigen.nig.ac.jp/barley/...
  63. pmc An isoform of eIF4E is a component of germ granules and is required for spermatogenesis in C. elegans
    A Amiri
    Department of Biology, Jordan Hall, 1001 E Third St, Indiana University, Bloomington, IN 47405 3700, USA
    Development 128:3899-912. 2001
    ..Our results suggest that C. elegans spermatogenesis requires either this specific isoform of eIF4E or an elevated level of eIF4E...
  64. ncbi Systematic approaches to using the FOX hunting system to identify useful rice genes
    Youichi Kondou
    RIKEN Plant Science Center, Yokohama, Kanagawa, Japan
    Plant J 57:883-94. 2009
    ..This technology provides a framework for the analysis of plant gene function in a heterologous host and of plant improvement by using heterologous gene resources...
  65. pmc Natural selection and adaptive evolution of leptin in the ochotona family driven by the cold environmental stress
    Jie Yang
    Key Laboratory of Qinghai Tibetan Plateau Biological Evolution and Adaptation, Northwest Institute of Plateau Biology, The Chinese Academy of Sciences, Xining, Qinghai, China
    PLoS ONE 3:e1472. 2008
    ..Variations in energy metabolism are remarkable for them living in cold environments. Leptin, an adipocyte-derived hormone, plays important roles in energy homeostasis...
  66. pmc A family of peptidoglycan recognition proteins in the fruit fly Drosophila melanogaster
    T Werner
    Umea Centre for Molecular Pathogenesis, Umea University, S 901 87 Umea, Sweden
    Proc Natl Acad Sci U S A 97:13772-7. 2000
    ..We demonstrate that at least PGRP-SA and SC1B can bind peptidoglycan, and a function in immunity is likely for this family...
  67. ncbi Genetical genomics: the added value from segregation
    R C Jansen
    Plant Research International BV, PO Box 16, NL 6700 AA, Wageningen, The Netherlands
    Trends Genet 17:388-91. 2001
    ..Genetical genomics will combine the power of two different worlds in a way that is likely to become instrumental in the further unravelling of metabolic, regulatory and developmental pathways...
  68. ncbi The human mitochondrial Mrs2 protein functionally substitutes for its yeast homologue, a candidate magnesium transporter
    G Zsurka
    Vienna Biocenter, Department of Microbiology and Genetics, University of Vienna, Dr Bohrgasse 9, Vienna, A 1030, Austria
    Genomics 72:158-68. 2001
    ..Like its yeast homologues, hsaMrs2p has been localized in mitochondria. The hsaMRS2 gene is located on chromosome 6 (6p22.1-p22.3) and is composed of 11 exons. A low level of the transcript is detected in various mouse tissues...
  69. ncbi Molecular cloning of human eotaxin, an eosinophil-selective CC chemokine, and identification of a specific eosinophil eotaxin receptor, CC chemokine receptor 3
    M Kitaura
    Shionogi Institute for Medical Science, Osaka, Japan
    J Biol Chem 271:7725-30. 1996
    ....
  70. pmc ZO-3, a novel member of the MAGUK protein family found at the tight junction, interacts with ZO-1 and occludin
    J Haskins
    Department of Cell Biology and Anatomy, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
    J Cell Biol 141:199-208. 1998
    ..In vitro affinity analyses demonstrated that recombinant 130-kD protein directly interacts with ZO-1 and the cytoplasmic domain of occludin, but not with ZO-2. We propose that this protein be named ZO-3...
  71. ncbi Acute transcriptional response of the honeybee peptide-antibiotics gene repertoire and required post-translational conversion of the precursor structures
    K Casteels-Josson
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021
    J Biol Chem 269:28569-75. 1994
    ..We speculate that this structure is a "co-drug," assembled by fusing "disulfide-rich" and "alpha-helical" class peptide antibiotics, a novel concept in naturally occurring antibacterials...
  72. ncbi Murine muscular dystrophy caused by a mutation in the laminin alpha 2 (Lama2) gene
    H Xu
    La Jolla Cancer Research Foundation, California 92037
    Nat Genet 8:297-302. 1994
    ..One mRNA is translated into an alpha 2 polypeptide with a deletion in domain VI. The truncated protein apparently lacks important qualities of the wild type protein and is unable to provide sufficient muscle stability...
  73. pmc Expression of the IRT1 metal transporter is controlled by metals at the levels of transcript and protein accumulation
    Erin L Connolly
    Department of Biological Sciences, Gilman 6044, Dartmouth College, Hanover, NH 03755, USA
    Plant Cell 14:1347-57. 2002
    ..Our results suggest that the expression of IRT1 is controlled by two distinct mechanisms that provide an effective means of regulating metal transport in response to changing environmental conditions...
  74. pmc Microbial community gene expression in ocean surface waters
    Jorge Frias-Lopez
    Departments of Civil and Environmental Engineering and Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 105:3805-10. 2008
    ..Thus, microbial community transcriptomic analyses revealed not only indigenous gene- and taxon-specific expression patterns but also gene categories undetected in previous DNA-based metagenomic surveys...
  75. ncbi Construction of infectious cDNA clones for dengue 2 virus: strain 16681 and its attenuated vaccine derivative, strain PDK-53
    R M Kinney
    Division of Vector Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado, USA
    Virology 230:300-8. 1997
    ..Although the NS3-250-Val mutation clearly affected virus replication in vitro, it was not a major determinant of attenuation for PDK-53 virus in suckling mice...
  76. ncbi A comprehensive expression analysis of the starch synthase gene family in rice (Oryza sativa L.)
    Tatsuro Hirose
    Department of Rice Research, National Agricultural Research Center, Joetsu, 943 0193 Niigata, Japan
    Planta 220:9-16. 2004
    ..The implications in this spatio-temporal work sharing of starch synthesis isogenes are discussed...
  77. ncbi DNMT1 binds HDAC2 and a new co-repressor, DMAP1, to form a complex at replication foci
    M R Rountree
    The Johns Hopkins Oncology Center, Tumor Biology Laboratory, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
    Nat Genet 25:269-77. 2000
    ..Thus, DNMT1 not only maintains DNA methylation, but also may directly target, in a heritable manner, transcriptionally repressive chromatin to the genome during DNA replication...
  78. pmc Identification of multiple cancer/testis antigens by allogeneic antibody screening of a melanoma cell line library
    Y T Chen
    Cornell University Medical College, New York Branch at Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Proc Natl Acad Sci U S A 95:6919-23. 1998
    ..Our findings document the immunogenicity of LAGE-1 and CT7 and emphasize the power of serological analysis of cDNA expression libraries in identifying new human tumor antigens...
  79. ncbi Intestinal water absorption through aquaporin 1 expressed in the apical membrane of mucosal epithelial cells in seawater-adapted Japanese eel
    Mayumi Aoki
    Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo, Tokyo 113 8657, Japan
    J Exp Biol 206:3495-505. 2003
    ....
  80. pmc Human macrophage-derived chemokine (MDC), a novel chemoattractant for monocytes, monocyte-derived dendritic cells, and natural killer cells
    R Godiska
    ICOS Corporation, Bothell, Washington 98021, USA
    J Exp Med 185:1595-604. 1997
    ..Unlike most other CC chemokines, MDC is encoded on human chromosome 16. MDC is thus a unique member of the CC chemokine family that may play a fundamental role in the function of dendritic cells, natural killer cells, and monocytes...
  81. pmc Isolation and functional analysis of Arabidopsis stress-inducible NAC transcription factors that bind to a drought-responsive cis-element in the early responsive to dehydration stress 1 promoter
    Lam Son Phan Tran
    Biological Resources Division, Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki 305 8686, Japan
    Plant Cell 16:2481-98. 2004
    ..However, erd1 was not upregulated in the transgenic plants. Other interacting factors may be necessary for the induction of erd1 in Arabidopsis under stress conditions...
  82. ncbi Latent transforming growth factor beta-binding protein 1 interacts with fibrillin and is a microfibril-associated protein
    Zenzo Isogai
    Department of Biochemistry and Molecular Biology, Shriners Hospital for Children, Oregon Health and Science University, Portland 97239, USA
    J Biol Chem 278:2750-7. 2003
    ..In tissues where LTBP-1 is not expressed, LTBP-4 may substitute for LTBP-1, because the C-terminal end of LTBP-4 binds equally well to fibrillin. A model depicting the relationship between LTBP-1 and fibrillin microfibrils is proposed...
  83. pmc Loss of glomerular foot processes is associated with uncoupling of podocalyxin from the actin cytoskeleton
    T Takeda
    Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, California, USA
    J Clin Invest 108:289-301. 2001
    ..PC/NHERF2/ezrin/actin interactions are disrupted in pathologic conditions associated with changes in GEC foot processes, indicating their importance for maintaining the unique organization of this epithelium...
  84. ncbi Autosomal recessive limb-girdle muscular dystrophy, LGMD2F, is caused by a mutation in the delta-sarcoglycan gene
    V Nigro
    Istituto di Patologia Generale e Oncologia, Facolta di Medicina, Seconda Universita degli Studi di Napoli, Italy
    Nat Genet 14:195-8. 1996
    ..We now show that a homozygous mutation in the delta SG gene (a single nucleotide deletion that alters its reading frame) is the cause of LGMD2F...
  85. ncbi Imaging neuronal activity during zebrafish behavior with a genetically encoded calcium indicator
    Shin Ichi Higashijima
    Department of Neurobiology and Behavior, State University of New York, Stony Brook, New York 11794 5230, USA
    J Neurophysiol 90:3986-97. 2003
    ..Transgenic lines such as the one we generated can also be crossed into mutant lines of fish to study both structural and functional consequences of the mutations...
  86. ncbi XTcf-3 transcription factor mediates beta-catenin-induced axis formation in Xenopus embryos
    M Molenaar
    Department of Immunology University Hospital, Utrecht The Netherlands
    Cell 86:391-9. 1996
    ..We propose that signaling by beta-catenin involves complex formation with XTcf-3, followed by nuclear translocation and activation of specific XTcf-3 target genes...
  87. pmc Cloning of a novel receptor expressed in rat prostate and ovary
    G G Kuiper
    Center for Biotechnology and Department of Medical Nutrition, Karolinska Institute, Huddinge, Sweden
    Proc Natl Acad Sci U S A 93:5925-30. 1996
    ..We conclude that clone 29 cDNA encodes a novel rat ER, which we suggest be named rat ERbeta to distinguish it from the previously cloned ER (ERalpha) from rat uterus...
  88. ncbi p29ING4 and p28ING5 bind to p53 and p300, and enhance p53 activity
    Masayuki Shiseki
    Laboratories of Human Carcinogenesis, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892 4255, USA
    Cancer Res 63:2373-8. 2003
    ..These results indicate that p29ING4 and p28ING5 may be significant modulators of p53 function...
  89. ncbi Mutations of the human homolog of Drosophila patched in the nevoid basal cell carcinoma syndrome
    H Hahn
    Centre for Molecular and Cell Biology, University of Queensland, St Lucia, Australia
    Cell 85:841-51. 1996
    ..We propose that a reduction in expression of the patched gene can lead to the developmental abnormalities observed in the syndrome and that complete loss of patched function contributes to transformation of certain cell types...
  90. ncbi Human very-low-density lipoprotein receptor complementary DNA and deduced amino acid sequence and localization of its gene (VLDLR) to chromosome band 9p24 by fluorescence in situ hybridization
    K Oka
    Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030
    Genomics 20:298-300. 1994
    A complementary DNA for the very-low-density lipoprotein receptor (VLDLR) that codes for a protein of 873 amino acids was cloned from a human heart cDNA library...
  91. pmc Hypersensitivity of DJ-1-deficient mice to 1-methyl-4-phenyl-1,2,3,6-tetrahydropyrindine (MPTP) and oxidative stress
    Raymond H Kim
    Campbell Family Institute for Breast Cancer Research, Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada M5G 2C1
    Proc Natl Acad Sci U S A 102:5215-20. 2005
    ..Thus, DJ-1 protects against neuronal oxidative stress, and loss of DJ-1 may lead to Parkinson's disease by conferring hypersensitivity to dopaminergic insults...
  92. ncbi A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma
    G Ambrosini
    Boyer Center for Molecular Medicine, Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06536, USA
    Nat Med 3:917-21. 1997
    ..These findings suggest that apoptosis inhibition may be a general feature of neoplasia and identify survivin as a potential new target for apoptosis-based therapy in cancer and lymphoma...
  93. pmc Evolution and expression of homeologous loci in Tragopogon miscellus (Asteraceae), a recent and reciprocally formed allopolyploid
    Jennifer A Tate
    Department of Botany, University of Florida, Gainesville, Florida 32611, USA
    Genetics 173:1599-611. 2006
    ..Synthetic F(1) hybrids between putative diploid progenitors are additive of their parental genomes, suggesting that polyploidization rather than hybridization induces genomic changes in Tragopogon...
  94. ncbi Molecular cloning and characterization of a full-length complementary DNA encoding human acid ceramidase. Identification Of the first molecular lesion causing Farber disease
    J Koch
    Institut fur Organische Chemie und Biochemie, D 53121 Bonn, Federal Republic of Germany
    J Biol Chem 271:33110-5. 1996
    ..A homoallelic point mutation (T222K) was also identified in the AC gene of a patient suffering from Farber disease, further confirming the authenticity of the full-length cDNA...
  95. ncbi Characterization of a hypoxia-inducible factor (HIF-1alpha ) from rainbow trout. Accumulation of protein occurs at normal venous oxygen tension
    A J Soitamo
    Turku Centre for Biotechnology, University of Turku, Abo Akademi University, FIN 20521 Turku, Finland
    J Biol Chem 276:19699-705. 2001
    ....
  96. ncbi Comparative analysis of ATRX, a chromatin remodeling protein
    Daniel J Park
    Department of Zoology, The University of Melbourne, Gate 12 Royal Parade, Melbourne 3010, Australia
    Gene 339:39-48. 2004
    ..Alignment of vertebrate ATRX sequences highlights other conserved elements, including a negatively charged mammalian sequence which we propose to be involved in binding of positively charged histone tails...
  97. ncbi Identification of a novel sarcoglycan gene at 5q33 encoding a sarcolemmal 35 kDa glycoprotein
    V Nigro
    Istituto di Patologia Generale ed Oncologia, Facolta di Medicina, Seconda Universita degli Studi di Napoli, Italy
    Hum Mol Genet 5:1179-86. 1996
    ..The gene spans at least 100 kb and is composed of eight exons. The identification of a novel sarcoglycan component modifies the current model of the dystrophin-glycoprotein complex...
  98. pmc Surface-enhanced laser desorption/ionization time-of-flight proteomic profiling of breast carcinomas identifies clinicopathologically relevant groups of patients similar to previously defined clusters from cDNA expression
    Kristyna Brozkova
    Masaryk Memorial Cancer Institute, Zluty kopec 7, 656 53 Brno, Czech Republic
    Breast Cancer Res 10:R48. 2008
    ....
  99. ncbi Dinoflagellate expressed sequence tag data indicate massive transfer of chloroplast genes to the nuclear genome
    Tsvetan R Bachvaroff
    Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA
    Protist 155:65-78. 2004
    ..A preliminary report of these data was presented at the Botany 2002 meeting in Madison, WI...
  100. ncbi Searching the expressed sequence tag (EST) databases: panning for genes
    C V Jongeneel
    Office of Information Technology, Ludwig Institute for Cancer Research and Swiss Institute of Bioinformatics, Chemin des Boveresses 155, CH 1066 Epalinges, Switzerland
    Brief Bioinform 1:76-92. 2000
    ..It also documents a collection of databases, software and web sites that can be useful to biologists interested in mining the EST databases over the Internet, or in establishing a local environment for such analyses...

Research Grants67

  1. True Direct Single Molecule RNA Sequencing
    PATRICE MARIE MILOS; Fiscal Year: 2010
    ..Yet transcriptome studies to date have relied on indirect measurements by sequencing complementary DNA (cDNA)...
  2. INTESTINAL AND COLONIC CYTOCHROME P450 GENE EXPRESSION
    Peter Traber; Fiscal Year: 1990
    ..P450 from which are expressed in small intestine and colon will be definitively identified using complementary DNA cloning...
  3. ANDROGEN RECEPTOR REGULATION OF REPRODUCTIVE FUNCTION
    Elizabeth Wilson; Fiscal Year: 1990
    Major advances in androgen receptor biochemistry require the isolation and sequencing of the complementary DNA (cDNA) for the androgen receptor...
  4. ANDROGEN RECEPTOR REGULATION OF REPRODUCTIVE FUNCTION
    Elizabeth Wilson; Fiscal Year: 1991
    Major advances in androgen receptor biochemistry require the isolation and sequencing of the complementary DNA (cDNA) for the androgen receptor...
  5. ANDROGEN RECEPTOR REGULATION OF REPRODUCTIVE FUNCTION
    Elizabeth Wilson; Fiscal Year: 1992
    Major advances in androgen receptor biochemistry require the isolation and sequencing of the complementary DNA (cDNA) for the androgen receptor...
  6. Immunoglobulin Somatic Mutation
    Ursula Storb; Fiscal Year: 2007
    ..Mismatch repair may remove some of the mutations or fix them by "correcting" the wildtype nucleotide on the complementary DNA strand. Cell replication propagates the mutations to one or both daughter cells...
  7. MUTATION OF SKELETAL TROPONIN C FUNCTIONAL DOMAINS
    James Potter; Fiscal Year: 1991
    ..The expression of a complementary DNA (cDNA) clone for chicken fast skeletal muscle TnC in bacteria will permit us to directly examine the role of ..
  8. EFFECTS OF SENSORY DEPRIVATION ON ADULT VISUAL CORTEX
    Edward Jones; Fiscal Year: 1999
    ..Results Complementary RNA probes derived from complementary DNA specifically subcloned from monkey tissue were used to localize, by in situ hybridization histochemistry, ..
  9. DEVELOPMENT OF DNA PROBES TO PERIODONTAL PATHOGENS
    BRUCE PASTER; Fiscal Year: 1990
    ..The second Aim is to design and construct complementary DNA probes to 16S rRNA by identifying unique 24-30 base regions of aligned sequences...
  10. RENIN-ANGIOTENSIN AND GROWTH IN URETERAL OBSTRUCTION
    ROBERT CHEVALIER; Fiscal Year: 1990
    ..Renin and angiotensinogen messenger RNA (mRNA) will be measured by complementary DNA (cDNA) probe, and renin release by individual renal cells will be determined by the reverse hemolytic plaque ..
  11. COMPARISON OF RAT & TROUT HEPATIC P450 INDUCTION
    JOHN LECH; Fiscal Year: 1991
    ..other P450 sequences; b) identification of P450 genes in addition to P450IA1 in trout by development of a complementary DNA (cDNA) probe corresponding to the highly-conserved P450 heme-binding region (P450hbr cDNA), by the use of ..
  12. COMPARISON OF RAT & TROUT HEPATIC P450 INDUCTION
    JOHN LECH; Fiscal Year: 1993
    ..other P450 sequences; b) identification of P450 genes in addition to P450IA1 in trout by development of a complementary DNA (cDNA) probe corresponding to the highly-conserved P450 heme-binding region (P450hbr cDNA), by the use of ..
  13. CONTROL OF TRANSCRIPTION OF ISOLATED EUCARYOTIC GENES
    Michael Holland; Fiscal Year: 1980
    ..plasmid DNAs containing structural genes which code for additional glycolytic enzymes will be isolated using complementary DNA synthesized from fractionated glycolytic messenger RNAs as hybridization "probes" for identifying E...
  14. Optical Antisense Breast Tumor Targeting
    Donald Hnatowich; Fiscal Year: 2007
    ..both in cell culture and in tumored animals when hybridized with a shorter BHQ3 inhibitor-conjugated complementary DNA but that fluorescence is expressed in the presence of the target mRNA in tumor as the duplex dissociates ..
  15. MATRIX DEGRADING PROTEINASES IN HUMAN SKIN DISORDERS
    Markku Kurkinen; Fiscal Year: 1990
    ..To this end, we have recently developed cloned complementary DNA (cDNA) probes for human collagenase and stromelysin...
  16. BIOLOGY OF CONNECTIVE TISSUE, BONES, AND TEETH
    Harold Slavkin; Fiscal Year: 1990
    ..of the dental non-collagenous extracellular matrix structural gene products, which are then used to produce complementary DNA probes (cDNA)...
  17. Multichannel Electrosonic Actuation Microarray for Cell-Based Screening
    JOHN MARK MEACHAM; Fiscal Year: 2010
    ..has the potential to accelerate discovery of potential cancer therapies through efficient screening of large complementary DNA (cDNA) and small interfering RNA (siRNA) libraries...
  18. ADA, ADENOSINE AND EMBRYO SURVIVAL
    Thomas Knudsen; Fiscal Year: 1993
    ..high-level reporter gene expression to antimesometrial decidual cells; (2) mutagenize functional human ADA complementary DNA (cDNA) sequences to mutant forms that retain catalytic activity yet are resistant to dCF; and (3) introduce ..
  19. COMPLEMENTARY DNA PROBES TO A STEROID RECEPTOR GENE
    J Pike; Fiscal Year: 1990
    ..We believe that these goals can be met most efficiently utilizing current molecular biologic approaches...
  20. DESIGN & SYNTHESIS OF OLIGONUCLEOTIDE ANALOG ANTIVIRALS
    Donald Bergstrom; Fiscal Year: 1991
    ..are planned to determine how these modifications affect the specificity and strength of hybridization to complementary DNA and RNA sequences...
  21. PATHOGENESIS OF PAGET'S DISEASE OF BONE
    MICHAEL VIOLA; Fiscal Year: 1980
    ..Virus-specific complementary DNA probes of high specific activity will be used in "in solution" molecular hybridization experiments to ..
  22. MOLECULAR BIOLOGY & FUNCTION OF PLATELET MEMBRANE GP130
    Peter Newman; Fiscal Year: 1990
    ..glycoproteins that are shared by platelets and endothelial cells, and have obtained a number of interesting complementary DNA (cDNA) clones...
  23. MOLECULAR BIOLOGY OF ALDOSE REDUCTASE AND CATARACT
    J Petrash; Fiscal Year: 1990
    ..b>Complementary DNA (cDNA) will be synthesized from bovine lens messenger RNA, and will be used to construct DNA libraries in ..
  24. Immunoglobulin Somatic Mutation
    Ursula Storb; Fiscal Year: 2006
    ..Mismatch repair may remove some of the mutations or fix them by "correcting" the wildtype nucleotide on the complementary DNA strand. Cell replication propagates the mutations to one or both daughter cells...
  25. Immunoglobulin Somatic Mutation
    Ursula Storb; Fiscal Year: 2003
    ..Mismatch repair may remove some of the mutations or fix them by "correcting" the wildtype nucleotide on the complementary DNA strand. Cell replication propagates the mutations to one or both daughter cells...
  26. ADENOSINE DEAMINASE--TARGET IN MOLECULAR TERATOGENESIS
    Thomas Knudsen; Fiscal Year: 1991
    ..Pertaining to the former, the objective is to localize the active sites of ADA synthesis. ADA-specific complementary DNA (cDNA) sequences will be used to probe the embryo, extraembryonic membranes and maternal decidua for ADA ..
  27. COMPARATIVE STUDY OF COAGULATION AND VASCULAR DISEASE
    WINIFRED DODDS; Fiscal Year: 1991
    ..canine models of vWD and hemophilia to study the FVIII/vWF complex including determination of the complementary DNA sequence for the canine vWF gene; (2) the action of thyroid hormone (T4) on vWF gene expression; and (3) ..
  28. Chimeric hepatitis C vaccine vector
    JACK NUNBERG; Fiscal Year: 2003
    ..Molecular analysis of these latter genera has been facilitated by the ability to manipulate the complementary DNA genomes of these RNA viruses and to generate infectious virions that can be analyzed in cell culture...
  29. Chimeric hepatitis C vaccine vector
    JACK NUNBERG; Fiscal Year: 2004
    ..Molecular analysis of these latter genera has been facilitated by the ability to manipulate the complementary DNA genomes of these RNA viruses and to generate infectious virions that can be analyzed in cell culture...
  30. COMPARATIVE STUDY OF COAGULATION AND VASCULAR DISEASE
    WINIFRED DODDS; Fiscal Year: 1993
    ..canine models of vWD and hemophilia to study the FVIII/vWF complex including determination of the complementary DNA sequence for the canine vWF gene; (2) the action of thyroid hormone (T4) on vWF gene expression; and (3) ..
  31. SCOR IN HEART FAILURE
    Robert Roberts; Fiscal Year: 1999
    ..b>Complementary DNA constructs with three known beta-myosin heavy chain (betaMHC) mutations will be expressed via E1-deficient ..
  32. ENZYME &GENE REPLACEMENT IN GM2 GANGLIOSIDOSIS
    MARIO RATTAZZI; Fiscal Year: 1990
    ..b>Complementary DNA encoding the beta-chain of human beta- hexosaminidase will be transfected into feline cultured fibroblasts ..
  33. Biochemical Basis of Somatic Hypermutation
    MYRON FREDRIC GOODMAN; Fiscal Year: 2010
    ..of AID- and A3G-catalyzed deaminations, which occur in a "haphazard" manner resulting in diverse mutations distributed throughout their DNA targets, immunoglobulin variable regions for AID, and HIV-1 complementary DNA for A3G.
  34. Biochemical Basis of Somatic Hypermutation
    MYRON GOODMAN; Fiscal Year: 2009
    ..of AID- and A3G-catalyzed deaminations, which occur in a "haphazard" manner resulting in diverse mutations distributed throughout their DNA targets, immunoglobulin variable regions for AID, and HIV-1 complementary DNA for A3G.
  35. WHITE BLOOD CELL OXIDASE EXPRESSION
    Peter Newburger; Fiscal Year: 1993
    ..The major tool for these experiments is complementary DNA (cDNA) from the recently cloned gene (termed "X-CGD") for X-linked CGD...
  36. WHITE BLOOD CELL OXIDASE IN LEUKEMIA AND NORMAL CELLS
    Peter Newburger; Fiscal Year: 1990
    ..The major tool for these experiments is complementary DNA (cDNA) from the recently cloned gene (termed "X-CGD") for X-linked CGD...
  37. PATHOBIOLOGY OF BREAST CANCER AND HORMONE RECEPTORS
    Michael Press; Fiscal Year: 1993
    ..b>Complementary DNA coding for ER and PR will be obtained from normal endometrial mRNA and compared with published sequences ..
  38. RELATEDNESS OF RNA TUMOR VIRUSES AND HUMAN NEOPLASIA
    James East; Fiscal Year: 1980
    ..An examination of their nucleotide sequence compositions as determined by cross-hybridization with complementary DNA transcripts of Gross murine leukemia virus and a rat endogenous retrovirus released from Wistar rat cells ..
  39. SYNTHESIS AND ANALYSIS OF HIV INTEGRASE INHIBITORS
    William Robinson; Fiscal Year: 1999
    ..Like all retroviruses, integration of a complementary DNA copy of the viral RNA genome into a host chromosome is absolutely required for productive and stable ..
  40. VIRUS SEQUENCES IN REV-TRANSFORMED CELLS
    HENRY BOSE; Fiscal Year: 1980
    ..b>Complementary DNA probes will be prepared against the unique sequence in the transforming virus (REV) and in the helper virus (..
  41. Post-Transcriptional Regulation of MMTV
    JAQUELIN DUDLEY; Fiscal Year: 2006
    ..and can be complemented by co-transfection of permissive cells with an infectious MMTV provirus or by rem complementary DNA. Moreover, the Rem C-terminus is not required for RNA export, but deletion of this domain increases export ..
  42. Post-Transcriptional Regulation of MMTV
    JAQUELIN DUDLEY; Fiscal Year: 2007
    ..and can be complemented by co-transfection of permissive cells with an infectious MMTV provirus or by rem complementary DNA. Moreover, the Rem C-terminus is not required for RNA export, but deletion of this domain increases export ..
  43. MOLECULAR STUDIES OF A KEY BILE ACID METABOLIZING ENZYME
    Andrew Stolz; Fiscal Year: 1991
    ..A full length complementary DNA (cDNA) clone for 3 alpha HSD will be isolated and sequenced from a rat liver specific cDNA library...
  44. MITOCHONDRIAL GENE EXPRESSION DURING OOGENESIS
    Andrew Webb; Fiscal Year: 1980
    ..RNA) isolated from early vs late oocytes will be undertaken using moleclar hybridization with radioactive complementary DNA (cDNA) synthesized from a template of total ovarian mt poly-A ion RNA...