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| complementary dnaSummarySummary: Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe. Top Publications
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Publications
Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genesJo Vandesompele
Center for Medical Genetics, Ghent University Hospital 1K5, De Pintelaan 185, B 9000 Ghent, Belgium
Genome Biol 3:RESEARCH0034. 2002....
Mutations in the DJ-1 gene associated with autosomal recessive early-onset parkinsonismVincenzo Bonifati
Genetic Epidemiologic Unit, Department of Clinical Genetics, Department of Epidemiology and Biostatistics, Erasmus Medical Center Rotterdam, Post Office Box 1738, 3000 DR Rotterdam, Netherlands
Science 299:256-9. 2003..Elucidating the physiological role of DJ-1 protein may promote understanding of the mechanisms of brain neuronal maintenance and pathogenesis of Parkinson's disease...- Normalization of cDNA microarray dataGordon K Smyth
Walter and Eliza Hall Institute, 1G Royal Parade, Parkville, VIC 3050, Australia
Methods 31:265-73. 2003..Variations on loess normalization include global loess normalization and two-dimensional normalization. Detailed commands are given to implement the normalization techniques using freely available software... - The TIGR Rice Genome Annotation Resource: improvements and new featuresShu Ouyang
The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
Nucleic Acids Res 35:D883-7. 2007..Enhanced data access is available through web interfaces, FTP downloads and a Data Extractor tool developed in order to support discrete dataset downloads... 
Profiling the HeLa S3 transcriptome using randomly primed cDNA and massively parallel short-read sequencingRyan Morin
Genome Sciences Centre, BC Cancer Agency, Vancouver, British Columbia, Canada
Biotechniques 45:81-94. 2008..Analysis of these variants, and previously unannotated splicing events in the HeLa S3 cell line, reveals an overrepresentation of gene categories including those previously implicated in cancer...- Gene discovery and annotation using LCM-454 transcriptome sequencingScott J Emrich
Bioinformatics and Computational Biology Graduate Program, Iowa State University, Ames, IA 50010, USA
Genome Res 17:69-73. 2007..We conclude that the coupling of LCM and 454 sequencing technologies facilitates the discovery of rare, possibly cell-type-specific transcripts... - Molecular cloning of NKp46: a novel member of the immunoglobulin superfamily involved in triggering of natural cytotoxicityA Pessino
Dipartimento di Medicina Sperimentale, Universita degli Studi di Genova, Italy
J Exp Med 188:953-60. 1998..In conclusion, this study reports the first characterization of the molecular structure of a NK-specific receptor involved in the mechanism of NK cell activation during natural cytotoxicity... - Osf2/Cbfa1: a transcriptional activator of osteoblast differentiationP Ducy
Department of Molecular Genetics, M D Anderson Cancer Center, University of Texas, Houston 77030, USA
Cell 89:747-54. 1997..This study identifies Osf2/Cbfa1 as an osteoblast-specific transcription factor and as a regulator of osteoblast differentiation... - Detoxification of the Fusarium mycotoxin deoxynivalenol by a UDP-glucosyltransferase from Arabidopsis thalianaBrigitte Poppenberger
Center of Applied Genetics, BOKU - University of Natural Resources and Applied Life Sciences, Muthgasse 18, A-1190 Vienna, Austria
J Biol Chem 278:47905-14. 2003..Constitutive overexpression in Arabidopsis leads to enhanced tolerance against deoxynivalenol... - The lateral organ boundaries gene defines a novel, plant-specific gene familyBin Shuai
Department of Botany and Plant Sciences, University of California, Riverside, CA 92521 0124, USA
Plant Physiol 129:747-61. 2002..The expression of LOB at the base of lateral organs suggests a potential role for LOB in lateral organ development... - Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signalingHiroshi Abe
Biological Resources Division, Japan International Research Center for Agricultural Sciences, 1 1 Ohwashi, Tsukuba, Ibaraki 305 8686, Japan
Plant Cell 15:63-78. 2003..These results indicate that both AtMYC2 and AtMYB2 proteins function as transcriptional activators in ABA-inducible gene expression under drought stress in plants... - Natural selection and adaptive evolution of leptin in the ochotona family driven by the cold environmental stressJie Yang
Key Laboratory of Qinghai Tibetan Plateau Biological Evolution and Adaptation, Northwest Institute of Plateau Biology, The Chinese Academy of Sciences, Xining, Qinghai, China
PLoS ONE 3:e1472. 2008..Variations in energy metabolism are remarkable for them living in cold environments. Leptin, an adipocyte-derived hormone, plays important roles in energy homeostasis... - An isoform of eIF4E is a component of germ granules and is required for spermatogenesis in C. elegansA Amiri
Department of Biology, Jordan Hall, 1001 E Third St, Indiana University, Bloomington, IN 47405 3700, USA
Development 128:3899-912. 2001..Our results suggest that C. elegans spermatogenesis requires either this specific isoform of eIF4E or an elevated level of eIF4E... - Genetical genomics: the added value from segregationR C Jansen
Plant Research International BV, PO Box 16, NL 6700 AA, Wageningen, The Netherlands
Trends Genet 17:388-91. 2001..Genetical genomics will combine the power of two different worlds in a way that is likely to become instrumental in the further unravelling of metabolic, regulatory and developmental pathways... - The human mitochondrial Mrs2 protein functionally substitutes for its yeast homologue, a candidate magnesium transporterG Zsurka
Vienna Biocenter, Department of Microbiology and Genetics, University of Vienna, Dr Bohrgasse 9, Vienna, A 1030, Austria
Genomics 72:158-68. 2001..Like its yeast homologues, hsaMrs2p has been localized in mitochondria. The hsaMRS2 gene is located on chromosome 6 (6p22.1-p22.3) and is composed of 11 exons. A low level of the transcript is detected in various mouse tissues... - Development of 5006 full-length CDNAs in barley: a tool for accessing cereal genomics resourcesKazuhiro Sato
Research Institute for Bioresources, Okayama University, Kurashiki, Japan
DNA Res 16:81-9. 2009..Sequence data from this article have been deposited with the DDBJ/EMBL/GenBank Data Libraries under accession nos AK248134-AK253139. The online database with annotation is available at http://www.shigen.nig.ac.jp/barley/... - A family of peptidoglycan recognition proteins in the fruit fly Drosophila melanogasterT Werner
Umea Centre for Molecular Pathogenesis, Umea University, S 901 87 Umea, Sweden
Proc Natl Acad Sci U S A 97:13772-7. 2000..We demonstrate that at least PGRP-SA and SC1B can bind peptidoglycan, and a function in immunity is likely for this family... - Systematic approaches to using the FOX hunting system to identify useful rice genesYouichi Kondou
RIKEN Plant Science Center, Yokohama, Kanagawa, Japan
Plant J 57:883-94. 2009..This technology provides a framework for the analysis of plant gene function in a heterologous host and of plant improvement by using heterologous gene resources... - Molecular cloning of human eotaxin, an eosinophil-selective CC chemokine, and identification of a specific eosinophil eotaxin receptor, CC chemokine receptor 3M Kitaura
Shionogi Institute for Medical Science, Osaka, Japan
J Biol Chem 271:7725-30. 1996.... - Cloning of mammalian Ire1 reveals diversity in the ER stress responsesX Z Wang
Skirball Institute of Biomolecular Medicine, the Departments of Medicine, Cell Biology and the Kaplan Cancer Center, NYU Medical Center, New York, NY 10016, USA
EMBO J 17:5708-17. 1998..These results indicate that a single upstream component, Ire1, plays a role in multiple facets of the ER stress-response in mammalian cells... - Construction of infectious cDNA clones for dengue 2 virus: strain 16681 and its attenuated vaccine derivative, strain PDK-53R M Kinney
Division of Vector Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado, USA
Virology 230:300-8. 1997..Although the NS3-250-Val mutation clearly affected virus replication in vitro, it was not a major determinant of attenuation for PDK-53 virus in suckling mice... - Identification of multiple cancer/testis antigens by allogeneic antibody screening of a melanoma cell line libraryY T Chen
Cornell University Medical College, New York Branch at Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
Proc Natl Acad Sci U S A 95:6919-23. 1998..Our findings document the immunogenicity of LAGE-1 and CT7 and emphasize the power of serological analysis of cDNA expression libraries in identifying new human tumor antigens... - Autosomal recessive limb-girdle muscular dystrophy, LGMD2F, is caused by a mutation in the delta-sarcoglycan geneV Nigro
Istituto di Patologia Generale e Oncologia, Facolta di Medicina, Seconda Universita degli Studi di Napoli, Italy
Nat Genet 14:195-8. 1996..We now show that a homozygous mutation in the delta SG gene (a single nucleotide deletion that alters its reading frame) is the cause of LGMD2F... - Human macrophage-derived chemokine (MDC), a novel chemoattractant for monocytes, monocyte-derived dendritic cells, and natural killer cellsR Godiska
ICOS Corporation, Bothell, Washington 98021, USA
J Exp Med 185:1595-604. 1997..Unlike most other CC chemokines, MDC is encoded on human chromosome 16. MDC is thus a unique member of the CC chemokine family that may play a fundamental role in the function of dendritic cells, natural killer cells, and monocytes... - Isolation and functional analysis of Arabidopsis stress-inducible NAC transcription factors that bind to a drought-responsive cis-element in the early responsive to dehydration stress 1 promoterLam Son Phan Tran
Biological Resources Division, Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki 305 8686, Japan
Plant Cell 16:2481-98. 2004..However, erd1 was not upregulated in the transgenic plants. Other interacting factors may be necessary for the induction of erd1 in Arabidopsis under stress conditions... - Loss of glomerular foot processes is associated with uncoupling of podocalyxin from the actin cytoskeletonT Takeda
Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, California, USA
J Clin Invest 108:289-301. 2001..PC/NHERF2/ezrin/actin interactions are disrupted in pathologic conditions associated with changes in GEC foot processes, indicating their importance for maintaining the unique organization of this epithelium... - DNMT1 binds HDAC2 and a new co-repressor, DMAP1, to form a complex at replication fociM R Rountree
The Johns Hopkins Oncology Center, Tumor Biology Laboratory, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Nat Genet 25:269-77. 2000..Thus, DNMT1 not only maintains DNA methylation, but also may directly target, in a heritable manner, transcriptionally repressive chromatin to the genome during DNA replication... - Genome-wide identification of human RNA editing sites by parallel DNA capturing and sequencingJin Billy Li
Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
Science 324:1210-3. 2009..This efficient approach greatly expands the repertoire of RNA editing targets and can be applied to studies involving RNA editing-related human diseases... - Latent transforming growth factor beta-binding protein 1 interacts with fibrillin and is a microfibril-associated proteinZenzo Isogai
Department of Biochemistry and Molecular Biology, Shriners Hospital for Children, Oregon Health and Science University, Portland 97239, USA
J Biol Chem 278:2750-7. 2003..In tissues where LTBP-1 is not expressed, LTBP-4 may substitute for LTBP-1, because the C-terminal end of LTBP-4 binds equally well to fibrillin. A model depicting the relationship between LTBP-1 and fibrillin microfibrils is proposed... - Imaging neuronal activity during zebrafish behavior with a genetically encoded calcium indicatorShin Ichi Higashijima
Department of Neurobiology and Behavior, State University of New York, Stony Brook, New York 11794 5230, USA
J Neurophysiol 90:3986-97. 2003..Transgenic lines such as the one we generated can also be crossed into mutant lines of fish to study both structural and functional consequences of the mutations... - Intestinal water absorption through aquaporin 1 expressed in the apical membrane of mucosal epithelial cells in seawater-adapted Japanese eelMayumi Aoki
Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo, Tokyo 113-8657, Japan
J Exp Biol 206:3495-505. 2003.... - A comprehensive expression analysis of the starch synthase gene family in rice (Oryza sativa L.)Tatsuro Hirose
Department of Rice Research, National Agricultural Research Center, Joetsu, 943 0193 Niigata, Japan
Planta 220:9-16. 2004..The implications in this spatio-temporal work sharing of starch synthesis isogenes are discussed... - Cloning of a novel receptor expressed in rat prostate and ovaryG G Kuiper
Center for Biotechnology and Department of Medical Nutrition, Karolinska Institute, Huddinge, Sweden
Proc Natl Acad Sci U S A 93:5925-30. 1996..We conclude that clone 29 cDNA encodes a novel rat ER, which we suggest be named rat ERbeta to distinguish it from the previously cloned ER (ERalpha) from rat uterus... - XTcf-3 transcription factor mediates beta-catenin-induced axis formation in Xenopus embryosM Molenaar
Department of Immunology University Hospital, Utrecht The Netherlands
Cell 86:391-9. 1996..We propose that signaling by beta-catenin involves complex formation with XTcf-3, followed by nuclear translocation and activation of specific XTcf-3 target genes... - Hypersensitivity of DJ-1-deficient mice to 1-methyl-4-phenyl-1,2,3,6-tetrahydropyrindine (MPTP) and oxidative stressRaymond H Kim
Campbell Family Institute for Breast Cancer Research, Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada M5G 2C1
Proc Natl Acad Sci U S A 102:5215-20. 2005..Thus, DJ-1 protects against neuronal oxidative stress, and loss of DJ-1 may lead to Parkinson's disease by conferring hypersensitivity to dopaminergic insults... - Mutations of the human homolog of Drosophila patched in the nevoid basal cell carcinoma syndromeH Hahn
Centre for Molecular and Cell Biology, University of Queensland, St Lucia, Australia
Cell 85:841-51. 1996..We propose that a reduction in expression of the patched gene can lead to the developmental abnormalities observed in the syndrome and that complete loss of patched function contributes to transformation of certain cell types... - p29ING4 and p28ING5 bind to p53 and p300, and enhance p53 activityMasayuki Shiseki
Laboratories of Human Carcinogenesis, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20892 4255, USA
Cancer Res 63:2373-8. 2003..These results indicate that p29ING4 and p28ING5 may be significant modulators of p53 function... - A novel anti-apoptosis gene, survivin, expressed in cancer and lymphomaG Ambrosini
Boyer Center for Molecular Medicine, Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06536, USA
Nat Med 3:917-21. 1997..These findings suggest that apoptosis inhibition may be a general feature of neoplasia and identify survivin as a potential new target for apoptosis-based therapy in cancer and lymphoma... - mRNA-Seq whole-transcriptome analysis of a single cellFuchou Tang
Wellcome Trust Cancer Research UK Gurdon Institute of Cancer and Developmental Biology, University of Cambridge, Cambridge, UK
Nat Methods 6:377-82. 2009..Finally, for Dicer1(-/-) and Ago2(-/-) (Eif2c2(-/-)) oocytes, we found that 1,696 and 1,553 genes, respectively, were abnormally upregulated compared to wild-type controls, with 619 genes in common... - Human very-low-density lipoprotein receptor complementary DNA and deduced amino acid sequence and localization of its gene (VLDLR) to chromosome band 9p24 by fluorescence in situ hybridizationK Oka
Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030
Genomics 20:298-300. 1994A complementary DNA for the very-low-density lipoprotein receptor (VLDLR) that codes for a protein of 873 amino acids was cloned from a human heart cDNA library... - Mutations in PEX1 are the most common cause of peroxisome biogenesis disordersB E Reuber
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
Nat Genet 17:445-8. 1997..These data demonstrate an important role for PEX1 in peroxisome biogenesis and suggest that mutations in this gene are the most common cause of the PBDs... - The molecular basis for the absence of N-glycolylneuraminic acid in humansA Irie
Department of Membrane Biochemistry, Tokyo Metropolitan Institute of Medical Science, Bunkyo ku, Tokyo 113 8613, Japan
J Biol Chem 273:15866-71. 1998..The absence of NeuGc in human glycoconjugates is due to a partial deletion in the gene that encodes CMP-NeuAc hydroxylase... - Dinoflagellate expressed sequence tag data indicate massive transfer of chloroplast genes to the nuclear genomeTsvetan R Bachvaroff
Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA
Protist 155:65-78. 2004..A preliminary report of these data was presented at the Botany 2002 meeting in Madison, WI... - Erythrocyte invasion by Babesia bovis merozoites is inhibited by polyclonal antisera directed against peptides derived from a homologue of Plasmodium falciparum apical membrane antigen 1Fasila R Gaffar
Department of Infectious Diseases and Immunology, Utrecht University, 3508 TD, Utrecht, The Netherlands
Infect Immun 72:2947-55. 2004.... - Expression of the bHLH transcription factor Tcf12 (ME1) gene is linked to the expansion of precursor cell populations during neurogenesisM Uittenbogaard
Department of Anatomy and Cell Biology, Program of Neuroscience, George Washington University Medical Center, 2300 I Street N W, Washington, DC 20037, USA
Brain Res Gene Expr Patterns 1:115-21. 2002.... - Characterization of a hypoxia-inducible factor (HIF-1alpha ) from rainbow trout. Accumulation of protein occurs at normal venous oxygen tensionA J Soitamo
Turku Centre for Biotechnology, University of Turku, Abo Akademi University, FIN 20521 Turku, Finland
J Biol Chem 276:19699-705. 2001.... - Genome sequence of Babesia bovis and comparative analysis of apicomplexan hemoprotozoaKelly A Brayton
Program in Genomics, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA
PLoS Pathog 3:1401-13. 2007..The genome sequence provides a greater understanding of B. bovis metabolism and potential avenues for drug therapies and vaccine development... - Comparative analysis of ATRX, a chromatin remodeling proteinDaniel J Park
Department of Zoology, The University of Melbourne, Gate 12 Royal Parade, Melbourne 3010, Australia
Gene 339:39-48. 2004..Alignment of vertebrate ATRX sequences highlights other conserved elements, including a negatively charged mammalian sequence which we propose to be involved in binding of positively charged histone tails... - Searching the expressed sequence tag (EST) databases: panning for genesC V Jongeneel
Office of Information Technology, Ludwig Institute for Cancer Research and Swiss Institute of Bioinformatics, Chemin des Boveresses 155, CH 1066 Epalinges, Switzerland
Brief Bioinform 1:76-92. 2000..It also documents a collection of databases, software and web sites that can be useful to biologists interested in mining the EST databases over the Internet, or in establishing a local environment for such analyses... - Characterization of multiple members of the HSP70 family in platyfish culture cells: molecular evolution of stress protein HSP70 in vertebratesMichiaki Yamashita
Institute for Frontier Medical Science, Kyoto University, Kyoto 606 8507, Japan
Gene 336:207-18. 2004.... - Survey of transcript expression in rainbow trout leukocytes reveals a major contribution of interferon-responsive genes in the early response to a rhabdovirus infectionCaroline O'Farrell
Unite de Virologie et Immunologie Moleculaires, Institut National de la Recherche Agronomique, 78352 Jouy en Josas Cedex, France
J Virol 76:8040-9. 2002..They were also expressed in vivo in experimentally infected fish, indicating their biological relevance in natural infection... - Diverse transcriptional response of CD4(+) T cells to stromal cell-derived factor (SDF)-1: cell survival promotion and priming effects of SDF-1 on CD4(+) T cellsY Suzuki
Experimental Retrovirology Section, Center for Cancer Research and HIV and AIDS Malignancy Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Immunol 167:3064-73. 2001..When examined using complementary DNA microarray, up-regulation of genes which are associated with DNA repair, detoxification, apoptosis, cell .. - CPEB is a specificity factor that mediates cytoplasmic polyadenylation during Xenopus oocyte maturationL E Hake
Worcester Foundation for Experimental Biology Shrewsbury, Massachusetts 01545
Cell 79:617-27. 1994..Partial restoration of polyadenylation in depleted extracts is achieved by the addition of CPEB, thus demonstrating that this protein is required for cytoplasmic polyadenylation... - Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thalianaTakeshi Itoh
Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305 8602, Japan
Genome Res 17:175-83. 2007..Our results suggest that natural selection may have played a role for duplicated genes in both species, so that duplication was suppressed or favored in a manner that depended on the function of a gene... - Murine muscular dystrophy caused by a mutation in the laminin alpha 2 (Lama2) geneH Xu
La Jolla Cancer Research Foundation, California 92037
Nat Genet 8:297-302. 1994..One mRNA is translated into an alpha 2 polypeptide with a deletion in domain VI. The truncated protein apparently lacks important qualities of the wild type protein and is unable to provide sufficient muscle stability... - Identification of stress-tolerance-related transcription-factor genes via mini-scale Full-length cDNA Over-eXpressor (FOX) gene hunting systemMiki Fujita
Gene Discovery Research Group, RIKEN Plant Science Center, 3 1 1 Koyadai, Tsukuba, Ibaraki 305 0074, Japan
Biochem Biophys Res Commun 364:250-7. 2007..Thus, our mini-scale FOX system may be used to screen for genes with valuable functions, such as transcription factors, from a small pool of genes that show similar expression profiles... - Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymeraseM Tewari
Department of Pathology, University of Michigan Medical School, Ann Arbor 48109, USA
Cell 81:801-9. 1995..Furthermore, CrmA blocked cleavage of PARP in cells undergoing apoptosis. We propose that Yama may represent an effector component of the mammalian cell death pathway and suggest that CrmA blocks apoptosis by inhibiting Yama... - CTCF, a conserved nuclear factor required for optimal transcriptional activity of the chicken c-myc gene, is an 11-Zn-finger protein differentially expressed in multiple formsE M Klenova
Institute of Carcinogenesis, Russian Cancer Research Center, Moscow
Mol Cell Biol 13:7612-24. 1993..Possible functions of other CTCF forms are discussed... - Evolution and expression of homeologous loci in Tragopogon miscellus (Asteraceae), a recent and reciprocally formed allopolyploidJennifer A Tate
Department of Botany, University of Florida, Gainesville, Florida 32611, USA
Genetics 173:1599-611. 2006..Synthetic F(1) hybrids between putative diploid progenitors are additive of their parental genomes, suggesting that polyploidization rather than hybridization induces genomic changes in Tragopogon... - Molecular cloning and characterization of a full-length complementary DNA encoding human acid ceramidase. Identification Of the first molecular lesion causing Farber diseaseJ Koch
Institut fur Organische Chemie und Biochemie, D 53121 Bonn, Federal Republic of Germany
J Biol Chem 271:33110-5. 1996..A homoallelic point mutation (T222K) was also identified in the AC gene of a patient suffering from Farber disease, further confirming the authenticity of the full-length cDNA... - Identification of a novel sarcoglycan gene at 5q33 encoding a sarcolemmal 35 kDa glycoproteinV Nigro
Istituto di Patologia Generale ed Oncologia, Facolta di Medicina, Seconda Universita degli Studi di Napoli, Italy
Hum Mol Genet 5:1179-86. 1996..The gene spans at least 100 kb and is composed of eight exons. The identification of a novel sarcoglycan component modifies the current model of the dystrophin-glycoprotein complex... - Surface-enhanced laser desorption/ionization time-of-flight proteomic profiling of breast carcinomas identifies clinicopathologically relevant groups of patients similar to previously defined clusters from cDNA expressionKristyna Brozkova
Masaryk Memorial Cancer Institute, Zluty kopec 7, 656 53 Brno, Czech Republic
Breast Cancer Res 10:R48. 2008.... - Molecular cloning and characterization of a cDNA encoding a novel cuticle protein in the silkworm, Bombyx moriHiroshi Sawada
Biological Laboratory, Center for Natural Science, Kitasato University, Kitasato 1 15 1, Sagamihara City, Kanagawa 228 8555, Japan
Comp Biochem Physiol B Biochem Mol Biol 134:519-27. 2003..We propose BMCPA may be a novel component of cuticle, and may play an important role in the integument of the larval silkworm... - Genomic organization and chromosomal localization of the Asna1 gene, a mouse homologue of a bacterial arsenic-translocating ATPase geneH Bhattacharjee
Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, 540 East Canfield Avenue, Detroit, MI 48201, USA
Gene 272:291-9. 2001..All splice sites conform to the GT-AG rule, except for the splice donor site of intron 4 that is GC instead of GT. Fluorescence in situ hybridization indicates that the Asna1 gene is localized in the C3-D1 region of mouse chromosome 8... - Oxygen-dependent expression of hypoxia-inducible factor-1alpha in renal medullary cells of ratsA P Zou
Departments of Physiology and Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
Physiol Genomics 6:159-68. 2001..Increased abundance of HIF-1alpha mRNA in the renal medulla may represent an adaptive response of renal medullary cells to low Po(2)... - Molecular cloning and characterization of a novel Jasmonate inducible pathogenesis-related class 10 protein gene, JIOsPR10, from rice (Oryza sativa L.) seedling leavesN S Jwa
Department of Molecular Biology, Sejong University, Seoul, 143-747, Korea
Biochem Biophys Res Commun 286:973-83. 2001..Induction of the JIOsPR10 gene expression by signaling molecules, PP inhibitors and pathogen attack, strongly indicate a role for this novel gene in rice self-defense/stress response(s)... - Cloning, mapping, and characterization of a human homologue of the yeast longevity assurance gene LAG1H Pan
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, China
Genomics 77:58-64. 2001..Furthermore, LASS2 protein was able to inhibit the colony formation of human hepatoma cells in vitro, which suggests that this gene may be involved in the regulation of cell growth... - [Molecular cloning for testis spermatogenesis cell apoptosis related gene TSARG1 and Mtsarg1 and expression analysis for Mtsarg1 gene]Jun Jiang Fu
Laboratory of Human Reproductive Engineering, Central South University Xiangya Medical College, Changsha 410078, China
Yi Chuan Xue Bao 30:25-9. 2003..Therefore, our results suggested that Mtsarg1 and TSARG1 would be pay potential roles in spermatogenesis cell apoptosis or spermatogenesis... - The murine cysteinyl leukotriene 2 (CysLT2) receptor. cDNA and genomic cloning, alternative splicing, and in vitro characterizationY Hui
Center for Experimental Therapeutics, Department of Pharmacology, University of Pennsylvania, Philadelphia, PA 19104, USA
J Biol Chem 276:47489-95. 2001..The identification of mCysLT(2)R will be useful for establishing CysLT(2)R-deficient mice and determining novel leukotriene functions... - Characterisation of RT1-E2, a multigenic family of highly conserved rat non-classical MHC class I molecules initially identified in cells from immunoprivileged sitesPierre Lau
IFR Claude de Préval, INSERM U563, CHU Purpan, 31300 Toulouse, France
BMC Immunol 4:7. 2003.... - Molecular cloning and characterization of AP-2 epsilon, a fifth member of the AP-2 familyRamakumar Tummala
Department of Biochemistry, State University of New York at Buffalo, 3435 Main Street, 121 Farber Hall, Buffalo, NY 14214, USA
Gene 321:93-102. 2003..Our study establishes AP-2 epsilon as a novel member of the AP-2 family, and suggests that it may play an important role in skin biology... - Characterization of the mouse liver fructose-1,6-bisphosphatase geneS Stein
Institute of Biochemistry, School of Medicine, University of Leipzig, Liebigstrasse 16, D 04103 Leipzig, Germany
Gene 264:215-24. 2001..An analysis of the steady state mRNA levels of liver and muscle FBPase in various mouse tissues was performed by Northern blotting and RT/PCR... - Adiponutrin, a transmembrane protein corresponding to a novel dietary- and obesity-linked mRNA specifically expressed in the adipose lineageS Baulande
Universite Pierre et Marie Curie, UMR Physiologie et Physiopathologie, CNRS, 15 rue de l Ecole de Medecine, 75270 Paris, Cedex 06, France
J Biol Chem 276:33336-44. 2001..This leads us to suggest that adiponutrin could be involved in vesicular targeting and protein transport restricted to the adipocyte function... - Cloning of a novel 2',5'-oligoadenylate synthetase-like molecule, Oasl5 in miceS Shibata
Division of Cell Biology, Center for Experimental Medicine, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
Gene 271:261-71. 2001..These findings suggest a novel function of Oasl5, which are independent of oligoadenylate synthetase activity, in the brain and developing embryos... - Molecular cloning and functional characterization of the canine androgen receptorB Lu
Department of Cardiovascular and Metabolic Diseases, Pfizer Global Research and Development, Groton, CT 06340, USA
Mol Cell Biochem 226:129-40. 2001..Together, these data indicate that we have cloned the canine AR and that its functional DNA binding and ligand binding domains are absolutely conserved with those reported for eight other species... - Gene structure and expression of phospholemman in mouseR C Bogaev
Department of Internal Medicine Cardiovascular Division, University of Virginia Health Sciences Center, Charlottesville, VA 22908, USA
Gene 271:69-79. 2001..Mouse PLM shares 93, 83 and 80% amino acid identity with rat, dog, and human PLMs, respectively. Tissue expression of murine PLM parallels that in other species, being highest in heart, skeletal muscle, and liver... - Cloning and expression analysis of a novel G-protein-coupled receptor selectively expressed on granulocytesS Yousefi
Novartis Horsham Research Centre, Horsham, West Sussex RH12 5AB, United Kingdom
J Leukoc Biol 69:1045-52. 2001..Using RT-PCR analysis, we showed more abundant expression of EX33 in neutrophils and eosinophils, in comparison with that in T- or B-lymphocytes, indicating cell-specific expression among leukocytes... - Myosin-VIIb, a novel unconventional myosin, is a constituent of microvilli in transporting epitheliaZ Y Chen
Department of Neurology, Howard Hughes Medical Institute, Boston, Massachusetts 02114, USA
Genomics 72:285-96. 2001..An antibody to myosin-VIIb labeled proximal tubule cells of the kidney and enterocytes of the intestine, specifically the distal tips of apical microvilli on these transporting epithelial cells... - The iodocyanopindolol and SM-11044 binding protein belongs to the TM9SF multispanning membrane protein superfamilyT Sugasawa
Sumitomo Pharmaceuticals Co, Ltd, Research Center, 1 98, Kasugade Naka 3 chome Konohana ku, 554, Osaka, Japan
Gene 273:227-37. 2001..Thus, SMBP is the first member of TM9SF with functional ligand binding properties, suggesting that some of these integral membrane proteins may function as channels, small molecule transporters or receptors... - Cloning and prokaryotic expression of a cDNA encoding a putative mitochondrial malate dehydrogenase in Oryza sativaChang-Fa Lin
Institute of Genetics, State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, People's Republic of China
DNA Seq 14:75-7. 2003..Its expression in E. coli BL21 as a fusion gene is studied further... - Genomic structure of the mouse PP4 gene: a developmentally regulated protein phosphataseM C Hu
Department of Molecular and Cellular Oncology, The University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
Gene 278:89-99. 2001..PP4 was differentially expressed in murine embryos at different developmental stages, suggesting that PP4 is a developmentally regulated protein phosphatase... - Identification of gastrin and multiple cholecystokinin genes in teleostTadahide Kurokawa
National Research Institute of Aquaculture, Fisheries Research Agency, 422 1, Nakatsuhama, Nansei, 516 0193, Mie, Japan
Peptides 24:227-35. 2003..The phylogenetic analysis shows that puffer and flounder gastrin classified into the vertebrate gastrin cluster and two types of CCK were probably produced by the genome duplication occurred in teleost phylogeny... - Molecular characterization of NSD1, a human homologue of the mouse Nsd1 geneN Kurotaki
Department of Human Genetics, Nagasaki University School of Medicine, Sakamoto 1 12 4, Nagasaki 852 8523, Japan
Gene 279:197-204. 2001..It remains to be investigated whether mutations of NSD1 lead to a specific phenotype in man... - [Cloning, expression and alternative splicing of a novel isoform of human TCP11b gene]Yong Xin Ma
The Key Laboratory of Biotherapy, Ministry of Education and Department of Medical Genetics, West China Hospital, Sichuan University, Chengdu 610041, China
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 35:182-8. 2003..Our results suggest that TCP11b gene may be important to human sperm function and male fertility... - Cloning of mouse Cited4, a member of the CITED family p300/CBP-binding transcriptional coactivators: induced expression in mammary epithelial cellsTetsuro Yahata
Department of Tumor Biology, Massachusetts General Hospital Cancer Center, Charlestown, Massachusetts 02129, USA
Genomics 80:601-13. 2002..These results implied possible roles for Cited4 in regulation of gene expression during development and differentiation of blood cells, endothelial cells, and mammary epithelial cells... - Molecular cloning of the fish interferon stimulated gene, 15 kDa (ISG15) orthologue: a ubiquitin-like gene induced by nephrotoxic damageMingjuan Liu
Molecular and Cellular Biology Program, University of Maryland Baltimore, 108 N Greene Street, Baltimore, MD 21201, USA
Gene 298:129-39. 2002.... - Expression pattern of alternatively spliced PECAM-1 isoforms in hematopoietic cells and plateletsYongji Wang
Department of Ophthalmology and Visual Sciences Madison, University of Wisconsin Medical School, Madison, Wisconsin 53792 4673, USA
J Cell Biochem 87:424-38. 2002..The regulated expression of these isoforms may be important during hematopoiesis and transendothelial migration... - Expression of brain-specific angiogenesis inhibitor 2 (BAI2) in normal and ischemic brain: involvement of BAI2 in the ischemia-induced brain angiogenesisHae Jin Kee
Research Institute of Medical Sciences, Chonnam National University, Kwangju, South Korea
J Cereb Blood Flow Metab 22:1054-67. 2002.... - Mouse PeP: a novel peroxisomal protein linked to myoblast differentiation and developmentAndreu Ferrer-Martínez
Institute of Molecular Medicine, School of Medicine, University of California, San Diego, California, USA
Dev Dyn 224:154-67. 2002..Thus our data suggest a connection between the function of PeP and murine cell differentiation and development... - New human sodium/glucose cotransporter gene (KST1): identification, characterization, and mutation analysis in ICCA (infantile convulsions and choreoathetosis) and BFIC (benign familial infantile convulsions) familiesPatrice Roll
INSERM U491, Faculte de Medecine de la Timone, 27 Bd J Moulin, 13385, Marseille Cedex 5, France
Gene 285:141-8. 2002..Due to its broad expression in human tissues, the human KST1 gene could be involved in several other diseases mapped to human chromosome 16p12-p11... - Characterization of germination-specific lipid transfer proteins from Euphorbia lagascaeJohan Edqvist
Department of Plant Biology, SLU, Box 7080, 750 07 Uppsala, Sweden
Planta 215:41-50. 2002..Both sequences contain eight cysteine residues, which are conserved in most plant LTPs. Expression analysis revealed that both genes were specifically expressed during germination... - Cloning and characterization of four apple MADS box genes isolated from vegetative tissueC G Van der Linden
Plant Research International BV, Wageningen University and Research Centre, Droevendaalsesteeg 1, PO Box 16, 6700 AA Wageningen, The Netherlands
J Exp Bot 53:1025-36. 2002..MdMADS12 is an AP1-like gene that is expressed at similar levels in leaves, vegetative shoots, and floral tissues, and that may be involved in the transition from the juvenile to the adult stage... - Molecular cloning and characterization of rat chemokine-like factor 1 and 2Yaxin Lou
Laboratory of Medical Immunology, School of Basic Medical Science, Peking University, Beijing, PR China
Gene 307:125-32. 2003..These findings show that rat CKLFs have similar bioactivity with human CKLFs, although they are different in tissue distribution and contain different characteristic motifs... - Cloning of rat thymic stromal lymphopoietin receptor (TSLPR) and characterization of genomic structure of murine Tslpr geneBlagoy Blagoev
Protein Interaction Laboratory, Center for Experimental Bioinformatics, University of Southern Denmark, Campusvej 55, M, DK 5230, Odense, Denmark
Gene 284:161-8. 2002..Finally, using linkage analysis, we mapped the murine Tslpr gene to mouse chromosome 5 between the Ecm2 and Pxn genes... - In Caenorhabditis elegans, the RNA-binding domains of the cytoplasmic polyadenylation element binding protein FOG-1 are needed to regulate germ cell fatesS W Jin
Department of Biology, University of Michigan, Ann Arbor, Michigan 48109, USA
Genetics 159:1617-30. 2001..On the basis of these data, we propose that FOG-1 binds RNA to regulate germ cell fates and that it does so by controlling the translation of its targets. One of these targets might be the fog-1 transcript itself... - A senescence-associated S-like RNase in the multicellular green alga Volvox carteriT Shimizu
Graduate School of Science, Kyoto University, Kyoto 606 8502, Japan
Gene 274:227-35. 2001..These observations imply that VRN1 may promote RNA degradation during senescence of somatic cells in V. carteri, and that its regulation has similarity with that of certain senescence-associated RNases in higher plants... - Molecular cloning and characterization of mouse cardiac triadin isoformsC S Hong
Department of Life Science, Kwangju Institute of Science and Technology, 1 Oryong dong, Puk Gu, Kwangju 500 712, South Korea
Gene 278:193-9. 2001..5 and 40 kDa. We termed those three isoforms as mouse cardiac triadin 1, mouse cardiac triadin 2 and mouse cardiac triadin 3... - Isolation and characterization of a novel gene from the DiGeorge chromosomal region that encodes for a mediator subunitL Berti
Department of Protein Biochemistry, Institute of Molecular Immunology GSF, Munich, 81377, Germany
Genomics 74:320-32. 2001..Together with the observed haploinsufficiency of PCQAP in DGS/VCFS patients, this finding is consistent with a possible role for this novel Mediator subunit in the development of some of the structures affected in DGS/VCFS... - Molecular cloning of feline interferon-gamma-inducing factor (interleukin-18) and its expression in various tissuesT Ishizaka
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Science, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, 113 8657, Tokyo, Japan
Vet Immunol Immunopathol 79:209-18. 2001..Expression of IL-18 mRNA was detected in various tissues including spleen, liver and cerebrum in the cat... - ZEC, a zinc finger protein with novel binding specificity and transcription regulatory activityGuo Yun Chen
Department of Biochemistry, Nagoya University Graduate School of Medicine, 65 Tsurumai Cho, Showa, Nagoya 466 8550, Japan
Gene 340:71-81. 2004..Furthermore, co-transfection assays demonstrated that ZEC could activate a reporter luciferase gene driven by this DNA sequence... - Human sphingosine kinase: molecular cloning, functional characterization and tissue distributionA J Melendez
Department of Molecular and Cellular Biology, Institut de Recherche Jouveinal Parke Davis, Fresnes, France
Gene 251:19-26. 2000..It is also expressed in brain and heart. In addition, database searches with the stSG2854 sequence indicate that huSPHK1 is also expressed in endothelial cells, retinal pigment epithelium, and senescent fibroblasts...
Research Grants
- True Direct Single Molecule RNA SequencingPATRICE MARIE MILOS; Fiscal Year: 2010..Yet transcriptome studies to date have relied on indirect measurements by sequencing complementary DNA (cDNA)...
- ANDROGEN RECEPTOR REGULATION OF REPRODUCTIVE FUNCTIONElizabeth Wilson; Fiscal Year: 1992Major advances in androgen receptor biochemistry require the isolation and sequencing of the complementary DNA (cDNA) for the androgen receptor...
- Immunoglobulin Somatic MutationUrsula Storb; Fiscal Year: 2007..Mismatch repair may remove some of the mutations or fix them by "correcting" the wildtype nucleotide on the complementary DNA strand. Cell replication propagates the mutations to one or both daughter cells...
- Optical Antisense Breast Tumor TargetingDonald Hnatowich; Fiscal Year: 2007..both in cell culture and in tumored animals when hybridized with a shorter BHQ3 inhibitor-conjugated complementary DNA but that fluorescence is expressed in the presence of the target mRNA in tumor as the duplex dissociates ..
- MOLECULAR BIOLOGY & FUNCTION OF PLATELET MEMBRANE GP130Peter Newman; Fiscal Year: 1990..glycoproteins that are shared by platelets and endothelial cells, and have obtained a number of interesting complementary DNA (cDNA) clones...
- MOLECULAR BIOLOGY OF ALDOSE REDUCTASE AND CATARACTJ Petrash; Fiscal Year: 1990..b>Complementary DNA (cDNA) will be synthesized from bovine lens messenger RNA, and will be used to construct DNA libraries in ..
- Immunoglobulin Somatic MutationUrsula Storb; Fiscal Year: 2006..Mismatch repair may remove some of the mutations or fix them by "correcting" the wildtype nucleotide on the complementary DNA strand. Cell replication propagates the mutations to one or both daughter cells...
- Immunoglobulin Somatic MutationUrsula Storb; Fiscal Year: 2003..Mismatch repair may remove some of the mutations or fix them by "correcting" the wildtype nucleotide on the complementary DNA strand. Cell replication propagates the mutations to one or both daughter cells...
- Chimeric hepatitis C vaccine vectorJACK NUNBERG; Fiscal Year: 2004..Molecular analysis of these latter genera has been facilitated by the ability to manipulate the complementary DNA genomes of these RNA viruses and to generate infectious virions that can be analyzed in cell culture...
- Biochemical Basis of Somatic HypermutationMYRON FREDRIC GOODMAN; Fiscal Year: 2010..of AID- and A3G-catalyzed deaminations, which occur in a "haphazard" manner resulting in diverse mutations distributed throughout their DNA targets, immunoglobulin variable regions for AID, and HIV-1 complementary DNA for A3G.
- PATHOBIOLOGY OF BREAST CANCER AND HORMONE RECEPTORSMichael Press; Fiscal Year: 1993..b>Complementary DNA coding for ER and PR will be obtained from normal endometrial mRNA and compared with published sequences ..
- Post-Transcriptional Regulation of MMTVJAQUELIN DUDLEY; Fiscal Year: 2007..and can be complemented by co-transfection of permissive cells with an infectious MMTV provirus or by rem complementary DNA. Moreover, the Rem C-terminus is not required for RNA export, but deletion of this domain increases export ..