complementary dna


Summary: Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.

Top Publications

  1. pmc Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
    Jo Vandesompele
    Center for Medical Genetics, Ghent University Hospital 1K5, De Pintelaan 185, B 9000 Ghent, Belgium
    Genome Biol 3:RESEARCH0034. 2002
  2. pmc The transcriptional landscape of the yeast genome defined by RNA sequencing
    Ugrappa Nagalakshmi
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    Science 320:1344-9. 2008
  3. ncbi The transcriptional landscape of the mammalian genome
    P Carninci
    Science 309:1559-63. 2005
  4. ncbi Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data
    Christian Ramakers
    Department of Anatomy and Embryology K2 283, Experimental and Molecular Cardiology Group, Academic Medical Centre, University of Amsterdam, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands
    Neurosci Lett 339:62-6. 2003
  5. pmc Transcriptome analysis by strand-specific sequencing of complementary DNA
    Dmitri Parkhomchuk
    Max Planck Institute for Molecular Genetics, Department of Vertebrate Genomics, Ihnestr 73, 14195 Berlin, Germany
    Nucleic Acids Res 37:e123. 2009
  6. pmc Improving the Arabidopsis genome annotation using maximal transcript alignment assemblies
    Brian J Haas
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Nucleic Acids Res 31:5654-66. 2003
  7. ncbi Normalization of cDNA microarray data
    Gordon K Smyth
    Walter and Eliza Hall Institute, 1G Royal Parade, Parkville, VIC 3050, Australia
    Methods 31:265-73. 2003
  8. pmc GeneWise and Genomewise
    Ewan Birney
    The European Bioinformatics Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK
    Genome Res 14:988-95. 2004
  9. pmc Simple cDNA normalization using kamchatka crab duplex-specific nuclease
    Pavel A Zhulidov
    Shemiakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, Miklukho Maklaya 16 10, 117871 Moscow, Russia
    Nucleic Acids Res 32:e37. 2004
  10. ncbi A global view of gene activity and alternative splicing by deep sequencing of the human transcriptome
    Marc Sultan
    Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Ihnestrasse 73, 14195 Berlin, Germany
    Science 321:956-60. 2008

Detail Information

Publications287 found, 100 shown here

  1. pmc Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
    Jo Vandesompele
    Center for Medical Genetics, Ghent University Hospital 1K5, De Pintelaan 185, B 9000 Ghent, Belgium
    Genome Biol 3:RESEARCH0034. 2002
  2. pmc The transcriptional landscape of the yeast genome defined by RNA sequencing
    Ugrappa Nagalakshmi
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    Science 320:1344-9. 2008
    ..We developed a quantitative sequencing-based method called RNA-Seq for mapping transcribed regions, in which complementary DNA fragments are subjected to high-throughput sequencing and mapped to the genome...
  3. ncbi The transcriptional landscape of the mammalian genome
    P Carninci
    Science 309:1559-63. 2005
    ..The data provide a comprehensive platform for the comparative analysis of mammalian transcriptional regulation in differentiation and development...
  4. ncbi Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data
    Christian Ramakers
    Department of Anatomy and Embryology K2 283, Experimental and Molecular Cardiology Group, Academic Medical Centre, University of Amsterdam, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands
    Neurosci Lett 339:62-6. 2003
    ..A computer program to perform this calculation is available on request (e-mail:; subject: LinRegPCR)...
  5. pmc Transcriptome analysis by strand-specific sequencing of complementary DNA
    Dmitri Parkhomchuk
    Max Planck Institute for Molecular Genetics, Department of Vertebrate Genomics, Ihnestr 73, 14195 Berlin, Germany
    Nucleic Acids Res 37:e123. 2009
    High-throughput complementary DNA sequencing (RNA-Seq) is a powerful tool for whole-transcriptome analysis, supplying information about a transcript's expression level and structure...
  6. pmc Improving the Arabidopsis genome annotation using maximal transcript alignment assemblies
    Brian J Haas
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Nucleic Acids Res 31:5654-66. 2003
    ..The algorithm of the Program to Assemble Spliced Alignments (PASA) tool is described, as well as the results of automated updates to Arabidopsis gene annotations...
  7. ncbi Normalization of cDNA microarray data
    Gordon K Smyth
    Walter and Eliza Hall Institute, 1G Royal Parade, Parkville, VIC 3050, Australia
    Methods 31:265-73. 2003
    ..Variations on loess normalization include global loess normalization and two-dimensional normalization. Detailed commands are given to implement the normalization techniques using freely available software...
  8. pmc GeneWise and Genomewise
    Ewan Birney
    The European Bioinformatics Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK
    Genome Res 14:988-95. 2004
    ..The GeneWise algorithm was developed from a principled combination of hidden Markov models (HMMs). Both algorithms are highly accurate and can provide both accurate and complete gene structures when used with the correct evidence...
  9. pmc Simple cDNA normalization using kamchatka crab duplex-specific nuclease
    Pavel A Zhulidov
    Shemiakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, Miklukho Maklaya 16 10, 117871 Moscow, Russia
    Nucleic Acids Res 32:e37. 2004
    ..We also employed DSN normalization to normalize cDNA from nervous tissues of the marine mollusc Aplysia californica (a popular model organism in neuroscience) to illustrate further the efficiency of the normalization technique...
  10. ncbi A global view of gene activity and alternative splicing by deep sequencing of the human transcriptome
    Marc Sultan
    Department of Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Ihnestrasse 73, 14195 Berlin, Germany
    Science 321:956-60. 2008
    ..A global survey of messenger RNA splicing events identified 94,241 splice junctions (4096 of which were previously unidentified) and showed that exon skipping is the most prevalent form of alternative splicing...
  11. pmc REPuter: the manifold applications of repeat analysis on a genomic scale
    S Kurtz
    Faculty of Technology, University of Bielefeld, PO Box 10 01 31, D 33501 Bielefeld, Germany
    Nucleic Acids Res 29:4633-42. 2001
  12. pmc The cotton ACTIN1 gene is functionally expressed in fibers and participates in fiber elongation
    Xue Bao Li
    College of Life Sciences, Central China Normal University, Wuhan 430079, China
    Plant Cell 17:859-75. 2005
    ..No defined actin network was observed in these fibers and, consequently, fiber elongation was inhibited. Our results suggested that GhACT1 plays an important role in fiber elongation but not fiber initiation...
  13. pmc Occludin: a novel integral membrane protein localizing at tight junctions
    M Furuse
    Department of Information Physiology, National Institute for Physiological Sciences, Aichi, Japan
    J Cell Biol 123:1777-88. 1993
    ..These findings revealed that an integral membrane protein localizing at tight junctions is now identified, which we designated as "occludin."..
  14. ncbi A small ubiquitin-related polypeptide involved in targeting RanGAP1 to nuclear pore complex protein RanBP2
    R Mahajan
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Cell 88:97-107. 1997
    ..Inhibition of nuclear protein import resulting from antibodies directed at NPC-associated RanGAP1 cannot be overcome by soluble cytosolic RanGAP1, indicating that GTP hydrolysis by Ran at RanBP2 is required for nuclear protein import...
  15. ncbi Characterization of a notochord-specific enhancer from the Brachyury promoter region of the ascidian, Ciona intestinalis
    J C Corbo
    Department of Biology, University of California, San Diego, La Jolla 92093 0347, USA
    Development 124:589-602. 1997
    ..We discuss the implications of this analysis with regard to the evolutionary conservation of integrative enhancers, and the subdivision of the axial and paraxial mesoderm in vertebrates...
  16. pmc Insights into corn genes derived from large-scale cDNA sequencing
    Nickolai N Alexandrov
    Ceres, Inc, 1535 Rancho Conejo Blvd, Thousand Oaks, CA 91320, USA
    Plant Mol Biol 69:179-94. 2009
    ..All of the sequence data from this study have been submitted to DDBJ/GenBank/EMBL under accession numbers EU940701-EU977132 (FLI cDNA) and FK944382-FL482108 (EST)...
  17. ncbi mRNA-Seq whole-transcriptome analysis of a single cell
    Fuchou Tang
    Wellcome Trust Cancer Research UK Gurdon Institute of Cancer and Developmental Biology, University of Cambridge, Cambridge, UK
    Nat Methods 6:377-82. 2009
    ..Finally, for Dicer1(-/-) and Ago2(-/-) (Eif2c2(-/-)) oocytes, we found that 1,696 and 1,553 genes, respectively, were abnormally upregulated compared to wild-type controls, with 619 genes in common...
  18. pmc Automated generation of heuristics for biological sequence comparison
    Guy St C Slater
    The Ensembl Group, EMBL European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SD, UK
    BMC Bioinformatics 6:31. 2005
  19. ncbi JIGSAW: integration of multiple sources of evidence for gene prediction
    Jonathan E Allen
    Center for Bioinformatics and Computational Biology, University of Maryland Institute for Advanced Computer Studies, College Park, MD 20742, USA
    Bioinformatics 21:3596-603. 2005
    ..Genome annotation pipelines collect a variety of types of evidence about gene structure and synthesize the results, which can then be refined further through manual, expert curation of gene models...
  20. ncbi A G-protein-coupled receptor for leukotriene B4 that mediates chemotaxis
    T Yokomizo
    Department of Biochemistry and Molecular Biology, Faculty of Medicine, The University of Tokyo, Japan
    Nature 387:620-4. 1997
    ..Here we report the cloning of the complementary DNA encoding a cell-surface LTB4 receptor that is highly expressed in human leukocytes...
  21. pmc A strand-specific RNA-Seq analysis of the transcriptome of the typhoid bacillus Salmonella typhi
    Timothy T Perkins
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
    PLoS Genet 5:e1000569. 2009
    ..Typhi OmpR regulon and identify novel OmpR regulated transcripts. Thus, ssRNA-seq provides a novel and powerful approach to the characterization of the bacterial transcriptome...
  22. ncbi Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR
    T D Schmittgen
    Department of Pharmaceutical Sciences, College of Pharmacy, and the Cancer Prevention and Research Center, Washington State University, Pullman, WA 99164 6534, USA
    J Biochem Biophys Methods 46:69-81. 2000
    ..The internal control gene needs to be properly validated when designing quantitative gene expression studies...
  23. ncbi Global identification of human transcribed sequences with genome tiling arrays
    Paul Bertone
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520 8103, USA
    Science 306:2242-6. 2004
    ..Transcribed sequences were located across the genome via hybridization to complementary DNA samples, reverse-transcribed from polyadenylated RNA obtained from human liver tissue...
  24. ncbi Real-time RT-PCR normalisation; strategies and considerations
    J Huggett
    Centre for Infectious Diseases and International Health, University College London, London, UK
    Genes Immun 6:279-84. 2005
    ..In this review we discuss the relative merits of different normalisation strategies and suggest a method of validation that will enable the measurement of biologically meaningful results...
  25. pmc The TIGR Rice Genome Annotation Resource: improvements and new features
    Shu Ouyang
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Nucleic Acids Res 35:D883-7. 2007
    ..Enhanced data access is available through web interfaces, FTP downloads and a Data Extractor tool developed in order to support discrete dataset downloads...
  26. pmc Microbial community gene expression in ocean surface waters
    Jorge Frias-Lopez
    Departments of Civil and Environmental Engineering and Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 105:3805-10. 2008
    ..Thus, microbial community transcriptomic analyses revealed not only indigenous gene- and taxon-specific expression patterns but also gene categories undetected in previous DNA-based metagenomic surveys...
  27. ncbi Cloning and expression of cDNA for a human enzyme that hydrolyzes 8-oxo-dGTP, a mutagenic substrate for DNA synthesis
    K Sakumi
    Medical Institute of Bioregulation, Faculty of Science, Kyushu University, Fukuoka, Japan
    J Biol Chem 268:23524-30. 1993
    ..In such cells, the frequency of spontaneous mutation was greatly reduced. We propose that the human 8-oxo-dGTPase protects genetic information from the untoward effects of endogenous oxygen radicals...
  28. ncbi Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs
    Y Okazaki
    1 Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center, RIKEN Yokohama Institute 1 7 22 Suehiro cho, Tsurumi ku, Yokohama, Kanagawa, 230 0045, Japan
    Nature 420:563-73. 2002
    ..Here we report the manual annotation of 60,770 full-length mouse complementary DNA sequences. These are clustered into 33,409 'transcriptional units', contributing 90...
  29. ncbi Detoxification of the Fusarium mycotoxin deoxynivalenol by a UDP-glucosyltransferase from Arabidopsis thaliana
    Brigitte Poppenberger
    Center of Applied Genetics, BOKU University of Natural Resources and Applied Life Sciences, Muthgasse 18, A 1190 Vienna, Austria
    J Biol Chem 278:47905-14. 2003
    ..Constitutive overexpression in Arabidopsis leads to enhanced tolerance against deoxynivalenol...
  30. pmc Gene expression patterns associated with blood-feeding in the malaria mosquito Anopheles gambiae
    Ali N Dana
    Center for Tropical Disease Research and Training, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA
    BMC Genomics 6:5. 2005
  31. ncbi Cambial meristem dormancy in trees involves extensive remodelling of the transcriptome
    Jarmo Schrader
    Department of Forest Genetics and Plant Physiology, Umea Plant Science Centre, Swedish University of Agricultural Sciences, 90183 Umea, Sweden
    Plant J 40:173-87. 2004
    ..The induction of genes such as poplar orthologues of FIE and HAP2 indicates a potential role for these global regulators of transcription in orchestrating extensive changes in gene expression during dormancy...
  32. ncbi Transcriptional maps of 10 human chromosomes at 5-nucleotide resolution
    Jill Cheng
    Affymetrix Inc, Santa Clara, CA 95051, USA
    Science 308:1149-54. 2005
    ..This organization has important implications for interpreting genotype-phenotype associations, regulation of gene expression, and the definition of a gene...
  33. pmc Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences
    Robert L Strausberg
    National Cancer Institute, Bethessda, MD 20892 2580, USA
    Proc Natl Acad Sci U S A 99:16899-903. 2002
    ..All MGC sequences and clones are available without restriction through public databases and clone distribution networks (see
  34. pmc Calcium-dependent protein kinases play an essential role in a plant defence response
    T Romeis
    The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, Norfolk, UK
    EMBO J 20:5556-67. 2001
    ..Silencing correlated with loss of CDPK mRNA, whereas mRNA accumulation of mitogen-activated protein kinase WIPK remained unaltered...
  35. ncbi Genome-wide identification of human RNA editing sites by parallel DNA capturing and sequencing
    Jin Billy Li
    Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Science 324:1210-3. 2009
    ..This efficient approach greatly expands the repertoire of RNA editing targets and can be applied to studies involving RNA editing-related human diseases...
  36. ncbi Transmembrane phosphoprotein Cbp regulates the activities of Src-family tyrosine kinases
    M Kawabuchi
    Division of Protein Metabolism, Institute for Protein Research, Osaka University, Suita, Japan
    Nature 404:999-1003. 2000
    ..These findings reveal Cbp as a new component of the regulatory mechanism controlling the activity of membrane-associated Src-PTKs...
  37. ncbi Profiling the HeLa S3 transcriptome using randomly primed cDNA and massively parallel short-read sequencing
    Ryan Morin
    Genome Sciences Centre, BC Cancer Agency, Vancouver, British Columbia, Canada
    Biotechniques 45:81-94. 2008
    ..Analysis of these variants, and previously unannotated splicing events in the HeLa S3 cell line, reveals an overrepresentation of gene categories including those previously implicated in cancer...
  38. pmc Transferability of the EST-SSRs developed on Nules clementine (Citrus clementina Hort ex Tan) to other Citrus species and their effectiveness for genetic mapping
    François L Luro
    INRA, Unité de Recherche GEQA, INRA San Giuliano, 20230 San Nicolao, France
    BMC Genomics 9:287. 2008
  39. pmc Envoplakin, a novel precursor of the cornified envelope that has homology to desmoplakin
    C Ruhrberg
    Keratinocyte Laboratory, Imperial Cancer Research Fund, London, United Kingdom
    J Cell Biol 134:715-29. 1996
    ..These data suggest that the 210-kD protein may link the cornified envelope to desmosomes and keratin filaments. We propose that the 210-kD protein be named "envoplakin."..
  40. pmc Isolation and analyses of genes preferentially expressed during early cotton fiber development by subtractive PCR and cDNA array
    Sheng Jian Ji
    National Laboratory of Protein Engineering and Plant Genetic Engineering and Peking Yale Joint Center for Plant Molecular Genetics and Agro Biotechnology, College of Life Sciences, Peking University, Beijing 100871, China
    Nucleic Acids Res 31:2534-43. 2003
    ..This report constitutes the first systematic analysis of genes involved in cotton fiber development. Our results suggest that a concerted mechanism involving multiple cellular pathways is responsible for cotton fiber elongation...
  41. ncbi Liver disintegration in the mouse embryo caused by deficiency in the RNA-editing enzyme ADAR1
    Jochen C Hartner
    Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Jahnstrasse 29, D 69120 Heidelberg, Germany
    J Biol Chem 279:4894-902. 2004
    ..Thus, ADAR1 subserves critical steps in developing non-nervous tissue, which are likely to include transcript editing...
  42. pmc Mapping the Burkholderia cenocepacia niche response via high-throughput sequencing
    D R Yoder-Himes
    Center for Microbial Ecology, Michigan State University, East Lansing, MI 48824, USA
    Proc Natl Acad Sci U S A 106:3976-81. 2009
    ..Compared with the CF strain, the soil strain shows a stronger global gene expression response to its environment, which is consistent with the need for a more dynamic reaction to the heterogeneous conditions of soil...
  43. ncbi Molecular cloning of a novel laminin chain, alpha 5, and widespread expression in adult mouse tissues
    J H Miner
    Department of Anatomy and Neurobiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    J Biol Chem 270:28523-6. 1995
    ..Analysis of RNA expression showed that alpha 5 is widely expressed in adult tissues, with highest levels in lung, heart, and kidney. Our results suggest that alpha 5 may be a major laminin chain of adult basal laminae...
  44. pmc Gene discovery and annotation using LCM-454 transcriptome sequencing
    Scott J Emrich
    Bioinformatics and Computational Biology Graduate Program, Iowa State University, Ames, IA 50010, USA
    Genome Res 17:69-73. 2007
    ..We conclude that the coupling of LCM and 454 sequencing technologies facilitates the discovery of rare, possibly cell-type-specific transcripts...
  45. pmc Afadin: A novel actin filament-binding protein with one PDZ domain localized at cadherin-based cell-to-cell adherens junction
    K Mandai
    Takai Biotimer Project, ERATO, Japan Science and Technology Corporation, c o JCR Pharmaceuticals Co, Ltd, Kobe 651 22, Japan
    J Cell Biol 139:517-28. 1997
    ..These results suggest that l-afadin serves as a linker of the actin cytoskeleton to the plasma membrane at cell-to-cell AJ...
  46. pmc Hypoxia-induced gene expression profiling in the euryoxic fish Gillichthys mirabilis
    A Y Gracey
    Hopkins Marine Station, Department of Biological Sciences, Stanford University, Pacific Grove, CA 93950 3094, USA
    Proc Natl Acad Sci U S A 98:1993-8. 2001
  47. pmc Analysis of microarray data using Z score transformation
    Chris Cheadle
    DNA Array Unit, Research Resources Branch, National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21221, USA
    J Mol Diagn 5:73-81. 2003
    ..The results provided by these methods can be as rigorous and are no more arbitrary than other test methods, and, in addition, they have the advantage that they can be easily adapted to standard spreadsheet programs...
  48. ncbi Plectasin is a peptide antibiotic with therapeutic potential from a saprophytic fungus
    Per H Mygind
    Novozymes A S, 2880 Bagsvaerd, Denmark
    Nature 437:975-80. 2005
    ..These findings identify fungi as a novel source of antimicrobial defensins, and show the therapeutic potential of plectasin. They also suggest that the defensins of insects, molluscs and fungi arose from a common ancestral gene...
  49. ncbi DNA deamination mediates innate immunity to retroviral infection
    Reuben S Harris
    Medical Research Council Laboratory of Molecular Biology, Hills Road, CB2 2QH, Cambridge, United Kingdom
    Cell 113:803-9. 2003
    ..These findings imply that targeted DNA deamination is a major strategy of innate immunity to retroviruses and likely also contributes to the sequence variation observed in many viruses (including HIV)...
  50. pmc Analysis and functional annotation of an expressed sequence tag collection for tropical crop sugarcane
    Andre L Vettore
    Centro de Biologia Molecular e Engenharia Genetica, Instituto da Computação, Universidade Estadual de Campinas, 13083 970 Campinas SP, Brazil
    Genome Res 13:2725-35. 2003
    ..This indicated that possibly 33,620 unique genes had been identified and indicated that >90% of the sugarcane expressed genes were tagged...
  51. pmc The lateral organ boundaries gene defines a novel, plant-specific gene family
    Bin Shuai
    Department of Botany and Plant Sciences, University of California, Riverside, CA 92521 0124, USA
    Plant Physiol 129:747-61. 2002
    ..The expression of LOB at the base of lateral organs suggests a potential role for LOB in lateral organ development...
  52. pmc Phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), a novel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase csk and is involved in regulation of T cell activation
    T Brdicka
    Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, 14220 Prague, Czech Republic
    J Exp Med 191:1591-604. 2000
    ..These findings collectively suggest that in the absence of external stimuli, the PAG-Csk complex transmits negative regulatory signals and thus may help to keep resting T cells in a quiescent state...
  53. ncbi Osf2/Cbfa1: a transcriptional activator of osteoblast differentiation
    P Ducy
    Department of Molecular Genetics, M D Anderson Cancer Center, University of Texas, Houston 77030, USA
    Cell 89:747-54. 1997
    ..This study identifies Osf2/Cbfa1 as an osteoblast-specific transcription factor and as a regulator of osteoblast differentiation...
  54. pmc Vinexin: a novel vinculin-binding protein with multiple SH3 domains enhances actin cytoskeletal organization
    N Kioka
    Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Cell Biol 144:59-69. 1999
    ..These data identify vinexin as a novel focal adhesion and cell- cell adhesion protein that binds via SH3 domains to the hinge region of vinculin, which can enhance actin cytoskeletal organization and cell spreading...
  55. pmc AceView: a comprehensive cDNA-supported gene and transcripts annotation
    Danielle Thierry-Mieg
    National Center for Biotechnology Information, National Library of Medicine, NIH, Bethesda, MD 20894, USA
    Genome Biol 7:S12.1-14. 2006
    ..The organizers evaluated the protein predictions in depth. We present a complementary analysis of the mRNAs, including alternative transcript variants...
  56. ncbi Complete sequencing and characterization of 21,243 full-length human cDNAs
    Toshio Ota
    Helix Research Institute, 1532 3 Yana, Kisarazu, Chiba 292 0812, Japan
    Nat Genet 36:40-5. 2004
    ..About one-fourth of them (1,378) showed a clear pattern of splicing. The distribution of GC content of noncoding cDNAs was narrow and had a peak at approximately 42%, relatively low compared with that of protein-coding cDNAs...
  57. pmc Clustering cancer gene expression data: a comparative study
    Marcilio C P De Souto
    Computational Molecular Biology, Max Planck Institute for Molecular Genetics, Berlin, Germany
    BMC Bioinformatics 9:497. 2008
    ..There have been no studies thus far performing a large-scale evaluation of different clustering methods in this context...
  58. ncbi A new class of membrane-bound chemokine with a CX3C motif
    J F Bazan
    Department of Molecular Biology, DNAX Research Institute, Palo Alto, California 94304, USA
    Nature 385:640-4. 1997
  59. ncbi Characterization of a novel salivary immunosuppressive protein from Ixodes ricinus ticks
    Gérard Leboulle
    pplied Genetics, Universite Libre de Bruxelles, rue des Professeurs Jeener et Brachet, 12, B 6041 Gosselies, Belgium
    J Biol Chem 277:10083-9. 2002
    ..In conclusion, these results suggest that Iris is an immunosuppressor, which might play an important role in the modulation of host immune response...
  60. ncbi Complete gene sequence of a human Puumala hantavirus isolate, Puumala Umeå/hu: sequence comparison and characterisation of encoded gene products
    Patrik Johansson
    Department of Medical Countermeasures, Division of NBC Defence, Swedish Defence Research Agency, SE 901 82 Umea, Sweden
    Virus Res 105:147-55. 2004
    ..Finally, the obtained results concerning the encoded gene products are of great importance for the design of new vaccines...
  61. pmc A functional genomics approach toward the understanding of secondary metabolism in plant cells
    Alain Goossens
    Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology, Ghent University, K L Ledeganckstraat 35, B 9000 Ghent, Belgium
    Proc Natl Acad Sci U S A 100:8595-600. 2003
    ..This method, which in addition to transcriptome data also generates gene tags, in the future might lead to the creation of novel tools for metabolic engineering of medicinal plant systems in general...
  62. pmc Automated Gene Ontology annotation for anonymous sequence data
    Steffen Hennig
    Max Planck Institute for Molecular Genetics, Ihnestrasse 73, D 14195 Berlin, Germany
    Nucleic Acids Res 31:3712-5. 2003
    ..The paper also addresses the reliability of automated GO annotations by using a reference set of more than 6000 human proteins. The GOblet server is accessible at
  63. ncbi DJ-1, a novel oncogene which transforms mouse NIH3T3 cells in cooperation with ras
    D Nagakubo
    Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan
    Biochem Biophys Res Commun 231:509-13. 1997
    ..Moreover, DJ-1 translocated from cytoplasm to nuclei in the S phase of the cell cycle. DJ-1 is thus suggested to be a novel mitogen-dependent oncogene product involved in a Ras-related signal transduction pathway...
  64. ncbi Epigenetic inactivation of a RAS association domain family protein from the lung tumour suppressor locus 3p21.3
    R Dammann
    Department of Biology, Beckman Research Institute, City of Hope Medical Center, Duarte, California, USA
    Nat Genet 25:315-9. 2000
    ..These characteristics indicate a potential role for RASSF1A as a lung tumour suppressor gene...
  65. ncbi Molecular cloning and heterologous expression of novel glucosyltransferases from tobacco cultured cells that have broad substrate specificity and are induced by salicylic acid and auxin
    G Taguchi
    Gene Research Center, Shinshu University, Ueda, Japan
    Eur J Biochem 268:4086-94. 2001
    ..These results might suggest that one of the roles of the products of these genes is scopoletin glucosylation, in response to salicylic acid and/or auxin, together with the other glucosyltransferases in tobacco cells...
  66. pmc PIG-B, a membrane protein of the endoplasmic reticulum with a large lumenal domain, is involved in transferring the third mannose of the GPI anchor
    M Takahashi
    Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Suita, Japan
    EMBO J 15:4254-61. 1996
    ..This autosomal location would explain why PIG-B is not involved in the defective GPI anchor synthesis in paroxysmal nocturnal hemoglobinuria, which is always caused by a somatic mutation of the X-linked PIG-A gene...
  67. ncbi Gain-of-function mutations and copy number increases of Notch2 in diffuse large B-cell lymphoma
    Suk young Lee
    Department of Cell Therapy and Transplantation Medicine, University of Tokyo Hospital, Tokyo 113 8655, Japan
    Cancer Sci 100:920-6. 2009
    ..These findings implicate Notch2 gain-of-function mutations in the pathogenesis of a subset of B-cell lymphomas, and suggest broader roles for Notch gene mutations in human cancers...
  68. ncbi Monitoring expression profiles of Arabidopsis gene expression during rehydration process after dehydration using ca 7000 full-length cDNA microarray
    Youko Oono
    Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, 3 1 1 Koyadai, Tsukuba 305 0074, Japan
    Plant J 34:868-87. 2003
    ..Functional analysis of rehydration-inducible and rehydration-repressed genes revealed their functions not only in the release from a stressed status but also in the recovery of growth in plants...
  69. ncbi Presence of ATG triplets in 5' untranslated regions of eukaryotic cDNAs correlates with a 'weak' context of the start codon
    I B Rogozin
    National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20804, USA
    Bioinformatics 17:890-900. 2001
    ..To reveal potential connections between the presence of upstream AUGs and other features of 5' UTRs, such as their length and the start codon context, we undertook a systematic analysis of the available eukaryotic 5' UTR sequences...
  70. ncbi Molecular cloning of human paxillin, a focal adhesion protein phosphorylated by P210BCR/ABL
    R Salgia
    Division of Hematologic Malignancies, Dana Farber Cancer Institute, Boston, Massachusetts 02115
    J Biol Chem 270:5039-47. 1995
    ..The predicted structure of paxillin implicates this molecule in protein-protein interactions involved in signal transduction from growth factor receptors and the BCR/ABL oncogene fusion protein to the cytoskeleton...
  71. ncbi FALSIFLORA, the tomato orthologue of FLORICAULA and LEAFY, controls flowering time and floral meristem identity
    N Molinero-Rosales
    Departamento de Biologia Aplicada, Area de Genetica, Universidad de Almeria, 04120 Almeria, Spain
    Plant J 20:685-93. 1999
    ..The function of this gene in comparison with other FLO/LFY orthologues is analysed in tomato, a plant with a sympodial growth habit and a cymose inflorescence development...
  72. pmc Whole genome shotgun sequencing of Brassica oleracea and its application to gene discovery and annotation in Arabidopsis
    Mulu Ayele
    The Institute for Genomic Research, Rockville, Maryland 20850, USA
    Genome Res 15:487-95. 2005
    ..Collectively our results and these data sets suggest that several thousand new Arabidopsis genes remain to be identified and annotated...
  73. pmc Whole genome analysis of genetic alterations in small DNA samples using hyperbranched strand displacement amplification and array-CGH
    José M Lage
    Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    Genome Res 13:294-307. 2003
    ..Gene-dosage alterations of threefold or more can be observed with high reproducibility with as few as 1000 cells of starting material...
  74. ncbi Titins: giant proteins in charge of muscle ultrastructure and elasticity
    S Labeit
    European Molecular Biology Laboratory, Heidelberg, Germany
    Science 270:293-6. 1995
    ..The complete complementary DNA sequence of human cardiac titin was determined...
  75. ncbi Multiple peroxisome proliferator-activated receptor beta subtypes from Atlantic salmon (Salmo salar)
    Michael J Leaver
    Institute of Aquaculture, University of Stirling, Stirling, UK
    J Mol Endocrinol 38:391-400. 2007
    ..The results indicate unexpected levels of variety and complexity in PPAR subtype and mechanism of action in lower vertebrates...
  76. ncbi CYP98A3 from Arabidopsis thaliana is a 3'-hydroxylase of phenolic esters, a missing link in the phenylpropanoid pathway
    G Schoch
    Department of Plant Stress Response, Institute of Plant Molecular Biology, CNRS UPR 2357, 28 rue Goethe, F 67083 Strasbourg cedex, France
    J Biol Chem 276:36566-74. 2001
    ..This hydroxylation occurs on depsides, the function of which was so far not understood, revealing an additional and unexpected level of networking in lignin biosynthesis...
  77. ncbi RNA expression patterns change dramatically in human neutrophils exposed to bacteria
    Y V Subrahmanyam
    Department of Genetics, Boyer Center for Molecular Medicine, Yale University School of Medicine, Yale University, New Haven, CT 06536-0812, USA
    Blood 97:2457-68. 2001
    ..Interruption of these changes by pathogens, such as Y pestis, could be responsible, at least in part, for the failure to contain infections by highly virulent organisms...
  78. ncbi Sequence characterization and expression patterns of defensin and lysozyme encoding genes from the gut of the reduviid bug Triatoma brasiliensis
    C A C Araújo
    Department of Special Zoology, Ruhr University, D 44780 Bochum, Germany
    Insect Biochem Mol Biol 36:547-60. 2006
    ..Comparing the levels in unfed fifth instars and up to 15 days after feeding, a strong def1 induction was evident in the fat body at 15 days after feeding and in the stomach a maximum level of def1 and lys1 at 5 days after feeding...
  79. pmc Centrosomal proteins CG-NAP and kendrin provide microtubule nucleation sites by anchoring gamma-tubulin ring complex
    Mikiko Takahashi
    Biosignal Research Center, Kobe University, Japan
    Mol Biol Cell 13:3235-45. 2002
    ..These results imply that CG-NAP and kendrin provide sites for microtubule nucleation in the mammalian centrosome by anchoring gamma-TuRC...
  80. ncbi Isolation and characterization of UGT2B15(Y85): a UDP-glucuronosyltransferase encoded by a polymorphic gene
    E Levesque
    Medical Research Council Group in Molecular Endocrinology, CHUL Research Center, Laval University, Quebec, Canada
    Pharmacogenetics 7:317-25. 1997
    ..The wide expression of UGT2B15 in many tissues indicates that it is a major glucuronidation enzyme in humans...
  81. ncbi Mutations in the DJ-1 gene associated with autosomal recessive early-onset parkinsonism
    Vincenzo Bonifati
    Genetic Epidemiologic Unit, Department of Clinical Genetics, Department of Epidemiology and Biostatistics, Erasmus Medical Center Rotterdam, Post Office Box 1738, 3000 DR Rotterdam, Netherlands
    Science 299:256-9. 2003
    ..Elucidating the physiological role of DJ-1 protein may promote understanding of the mechanisms of brain neuronal maintenance and pathogenesis of Parkinson's disease...
  82. pmc Polyphenol oxidase from hybrid poplar. Cloning and expression in response to wounding and herbivory
    C P Constabel
    Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada TGG 2E9
    Plant Physiol 124:285-95. 2000
    ..This wound- and herbivore-induced expression of PPO in hybrid poplar supports the defensive role of this protein against insect pests...
  83. ncbi A novel jasmonic acid-inducible rice myb gene associates with fungal infection and host cell death
    M W Lee
    Department of Plant Pathology, University of Arkansas, Fayetteville 72701, USA
    Mol Plant Microbe Interact 14:527-35. 2001
    ..These results suggest that JAmyb is related closely to host cell death and is involved in the JA-mediated, SA-independent signaling pathways in rice...
  84. pmc Inhibition of vascular endothelial cell growth factor activity by an endogenously encoded soluble receptor
    R L Kendall
    Department of Biochemistry, Merck Research Laboratories, Rahway, NJ 07065
    Proc Natl Acad Sci U S A 90:10705-9. 1993
  85. pmc MYB98 is required for pollen tube guidance and synergid cell differentiation in Arabidopsis
    Ryushiro D Kasahara
    Department of Biology, University of Utah, Salt Lake City, Utah 84112 0840, USA
    Plant Cell 17:2981-92. 2005
    ..Together, these data suggest that MYB98 controls the development of specific features within the synergid cell during female gametophyte development...
  86. pmc Analysis of small human proteins reveals the translation of upstream open reading frames of mRNAs
    Masaaki Oyama
    Human Genome Center, Institute of Medical Science, University of Tokyo, Minato ku, Tokyo 108 8639, Japan
    Genome Res 14:2048-52. 2004
    ..This investigation provides the first direct evidence of translation of upstream ORFs in human cells, which could greatly change the current outline of the human proteome...
  87. ncbi Discovery of the genes in response to white spot syndrome virus (WSSV) infection in Fenneropenaeus chinensis through cDNA microarray
    Bing Wang
    Institute of Oceanology, Chinese Academy of Sciences, Qingdao, People s Republic of China
    Mar Biotechnol (NY) 8:491-500. 2006
    ..As a substitute, we found that triosephosphate isomerase (TPI) was an ideal candidate of interstandards in this situation...
  88. ncbi Large-scale statistical analysis of secondary xylem ESTs in pine
    Nathalie Pavy
    ARBOREA and Centre de Recherche en Biologie Forestiére, Universite Laval, Pavillon Charles Eugène Marchand, Sainte Foy, Que, G1K 7P4, Canada
    Plant Mol Biol 57:203-24. 2005
    ..Thus, we illustrated the potential of extracting information from conifer sequences already accessible through well-structured public databases...
  89. ncbi Natural killer cell receptors in the horse: evidence for the existence of multiple transcribed LY49 genes
    Tomoko Takahashi
    Department of Biosystems Science, School of Advanced Sciences, The Graduate University for Advanced Studies Sokendai, Hayama, Japan
    Eur J Immunol 34:773-84. 2004
    ..We also show that horse spleen expresses ILT-like genes with unique domain organizations. Radiation hybrid mapping and fluorescence in situ hybridization localized horse LY49 and KIR/ILT genes to chromosomes 6q13 and 10p12, respectively...
  90. ncbi Migfilin and Mig-2 link focal adhesions to filamin and the actin cytoskeleton and function in cell shape modulation
    Yizeng Tu
    Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Cell 113:37-47. 2003
    ..Together, Mig-2, migfilin and filamin define a connection between cell matrix adhesions and the actin cytoskeleton and participate in the orchestration of actin assembly and cell shape modulation...
  91. ncbi The FHIT gene, spanning the chromosome 3p14.2 fragile site and renal carcinoma-associated t(3;8) breakpoint, is abnormal in digestive tract cancers
    M Ohta
    Kimmel Cancer Center, Jefferson Medical College, Philadelphia, Pennsylvania, USA
    Cell 84:587-97. 1996
    ..Aberrant transcripts of the FHIT locus were found in approximately 50% of esophageal, stomach, and colon carcinomas...
  92. ncbi Overexpression of the cell adhesion molecules DDR1, Claudin 3, and Ep-CAM in metaplastic ovarian epithelium and ovarian cancer
    Viola A Heinzelmann-Schwarz
    Cancer Research Program, Garvan Institute of Medical Research, Darlinghurst, Australia
    Clin Cancer Res 10:4427-36. 2004
    ..The aims of this study were to integrate the results from 14 transcript profiling studies of EOC to identify novel biomarkers and to examine their expression in early and late stages of the disease...
  93. ncbi Gene expression analysis of human thymoma correlates with tumor stage
    Hidefumi Sasaki
    Department of Surgery II, Nagoya City University Medical School, Nagoya, Japan
    Int J Cancer 101:342-7. 2002
    ..type B1, B2 or AB). The combined use of oligonucleotide microarray and real-time RT-PCR analyses provides a powerful new approach to elucidate the in vivo molecular events correlated with tumor stage of thymoma...
  94. ncbi SINEs and LINEs share common 3' sequences: a review
    N Okada
    Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan
    Gene 205:229-43. 1997
    ..This organization of SINEs and LINEs provides the basis for a simple general scheme by which SINEs might acquire retropositional activity...
  95. pmc A widely expressed betaIII spectrin associated with Golgi and cytoplasmic vesicles
    M C Stankewich
    Department of Pathology, Yale University, New Haven, CT 06520, USA
    Proc Natl Acad Sci U S A 95:14158-63. 1998
    ..Liver Golgi membranes and other vesicular compartment markers cosediment in vitro with betaIII spectrin. betaIII spectrin thus constitutes a major component of the Golgi and vesicular membrane skeletons...
  96. ncbi Overexpression of spermidine synthase enhances tolerance to multiple environmental stresses and up-regulates the expression of various stress-regulated genes in transgenic Arabidopsis thaliana
    Yoshihisa Kasukabe
    Toyobo Research Center Co Ltd, Katata, Ohtsu, Shiga, 520 0292 Japan
    Plant Cell Physiol 45:712-22. 2004
    ..These results strongly suggest an important role for spermidine as a signaling regulator in stress signaling pathways, leading to build-up of stress tolerance mechanisms in plants under stress conditions...
  97. ncbi Two zebrafish eIF4E family members are differentially expressed and functionally divergent
    Javier Robalino
    Center of Marine Biotechnology, Baltimore, Maryland 21202, USA
    J Biol Chem 279:10532-41. 2004
  98. ncbi An excitatory amino-acid transporter with properties of a ligand-gated chloride channel
    W A Fairman
    Howard Hughes Medical Institute, Oregon Health Sciences University, Portland 97201, USA
    Nature 375:599-603. 1995
    ..Thus EAAT4 combines the re-uptake of neurotransmitter with a mechanism for increasing chloride permeability, both of which could regulate excitatory neurotransmission...
  99. ncbi Grap is a novel SH3-SH2-SH3 adaptor protein that couples tyrosine kinases to the Ras pathway
    G S Feng
    Department of Biochemistry and Molecular Biology, Walther Oncology Center, Indiana University School of Medicine, Indianapolis 46202 5121, USA IUPUI EDU
    J Biol Chem 271:12129-32. 1996
    ..These results show that a family of Grb2-like proteins exist and couple signals from receptor and cytoplasmic tyrosine kinases to the Ras signaling pathway...
  100. ncbi Isolation and characterization of the faciogenital dysplasia (Aarskog-Scott syndrome) gene: a putative Rho/Rac guanine nucleotide exchange factor
    N G Pasteris
    Department of Human Genetics, University of Michigan, Ann Arbor 48109 0688
    Cell 79:669-78. 1994
    ..These results provide compelling evidence that FGD1 is responsible for FGDY and suggest that FGD1 is a Rho/RacGEF involved in mammalian development...

Research Grants62

  1. Vaccine Development for Falciparum Malaria in Pregnant Women and Their Offspring
    Ian C Michelow; Fiscal Year: 2013
    ..falciparum (3D7) malaria genomic DNA T7 phage display library as well as a wild type blood-stage complementary DNA T7 phage library for this purpose...
  2. Validation of microRNAs as therapeutic targets in hematological malignancies
    Natarajan Muthusamy; Fiscal Year: 2013
    ..To date mRNA targeted therapies have relied mostly on antisense or reverse complementary DNA ODN-based approaches...
  3. Biochemical Basis of Somatic Hypermutation
    MYRON GOODMAN; Fiscal Year: 2013
    ..of AID- and A3G-catalyzed deaminations, which occur in a "haphazard" manner resulting in diverse mutations distributed throughout their DNA targets, immunoglobulin variable regions for AID, and HIV-1 complementary DNA for A3G.
  4. Multichannel Electrosonic Actuation Microarray for Cell-Based Screening
    JOHN MARK MEACHAM; Fiscal Year: 2010
    ..has the potential to accelerate discovery of potential cancer therapies through efficient screening of large complementary DNA (cDNA) and small interfering RNA (siRNA) libraries...
  5. Microfluidic platform for rapid influenza subtyping
    Anubhav Tripathi; Fiscal Year: 2010
    ..from mucosal swabs and isolates influenza virus RNA (vRNA) based on conserved consensus sequences to complementary DNA oligonucleotides immobilized on microbeads...
    Peter Traber; Fiscal Year: 1993
    ..P450 from which are expressed in small intestine and colon will be definitively identified using complementary DNA cloning...
  7. Discovery and characterization of new natural products from uncultured bacteria
    Sean F Brady; Fiscal Year: 2013
    ..Two related but complementary DNA-based screening approaches will then be used to recover novel small molecule biosynthetic gene clusters from ..
  8. Immunoglobulin Somatic Mutation
    Ursula Storb; Fiscal Year: 2007
    ..Mismatch repair may remove some of the mutations or fix them by "correcting" the wildtype nucleotide on the complementary DNA strand. Cell replication propagates the mutations to one or both daughter cells...
  9. GammaPNA Miniprobes for Telomere FISH
    Tumul Srivastava; Fiscal Year: 2013
    ..The basis of gammaPNA miniprobes is the high affinity with which gammaPNA hybridizes to complementary DNA. The specific application addressed in this proposal is telomere analysis, which is currently done using ..
    ROBERT CHEVALIER; Fiscal Year: 1991
    ..Renin and angiotensinogen messenger RNA (mRNA) will be measured by complementary DNA (cDNA) probe, and renin release by individual renal cells will be determined by the reverse hemolytic plaque ..
    JOHN LECH; Fiscal Year: 1993
    ..other P450 sequences; b) identification of P450 genes in addition to P450IA1 in trout by development of a complementary DNA (cDNA) probe corresponding to the highly-conserved P450 heme-binding region (P450hbr cDNA), by the use of ..
    Michael Holland; Fiscal Year: 1980
    ..plasmid DNAs containing structural genes which code for additional glycolytic enzymes will be isolated using complementary DNA synthesized from fractionated glycolytic messenger RNAs as hybridization "probes" for identifying E...
    J Pike; Fiscal Year: 1992
    ..We believe that these goals can be met most efficiently utilizing current molecular biologic approaches...
    Thomas Knudsen; Fiscal Year: 1993
    ..Pertaining to the former, the objective is to localize the active sites of ADA synthesis. ADA-specific complementary DNA (cDNA) sequences will be used to probe the embryo, extraembryonic membranes and maternal decidua for ADA ..
  15. Chimeric hepatitis C vaccine vector
    JACK NUNBERG; Fiscal Year: 2004
    ..Molecular analysis of these latter genera has been facilitated by the ability to manipulate the complementary DNA genomes of these RNA viruses and to generate infectious virions that can be analyzed in cell culture...
    MARIO RATTAZZI; Fiscal Year: 1990
    ..b>Complementary DNA encoding the beta-chain of human beta- hexosaminidase will be transfected into feline cultured fibroblasts ..
    Donald Bergstrom; Fiscal Year: 1993
    ..are planned to determine how these modifications affect the specificity and strength of hybridization to complementary DNA and RNA sequences...
    Peter Newburger; Fiscal Year: 1991
    ..The major tool for these experiments is complementary DNA (cDNA) from the recently cloned gene (termed "X-CGD") for X-linked CGD...
    Andrew Stolz; Fiscal Year: 1993
    ..A full length complementary DNA (cDNA) clone for 3 alpha HSD will be isolated and sequenced from a rat liver specific cDNA library...
    Marila Cordeiro Stone; Fiscal Year: 2004
    ..A T:T mismatch nearby the dieter site will serve as a sequence marker for the complementary DNA strand...
    WINIFRED DODDS; Fiscal Year: 1993
    ..canine models of vWD and hemophilia to study the FVIII/vWF complex including determination of the complementary DNA sequence for the canine vWF gene; (2) the action of thyroid hormone (T4) on vWF gene expression; and (3) ..
    William Robinson; Fiscal Year: 1999
    ..Like all retroviruses, integration of a complementary DNA copy of the viral RNA genome into a host chromosome is absolutely required for productive and stable ..
  23. Adducts of mitomycin C with nucleotides.
    Maria Tomasz; Fiscal Year: 2006
    ..One of these is a DNA interstrand cross-link, as a result of bifunctional alkylation of the complementary DNA strands by MC, while the others are adducts of the drug, linked to only one strand of DNA (monoadducts and ..
    PATRICK KANDA; Fiscal Year: 1991
    ..derives from analysis of its primary amino acid sequence, which has been predicted from the envelope gene complementary DNA sequence. Computer algorithms will be used to predict possible epitopes associated with gp 160...
    JACOB GHAZARIAN; Fiscal Year: 1990
    ..between the two hydroxylases will be studied by the technique of differential hybridization and cloning of complementary DNA by insertion into a vector plasmid and transfecting a host bacterium...
    ROSALIA SIMMEN; Fiscal Year: 1990
    ..The complementary DNA (cDNA) for Uf will be isolated from a Lambdagt 11 expression library prepared from porcine uterine ..
    Thomas James; Fiscal Year: 2001 each position, and 2'-O-alkoxy derivatives, will be compared with the RNA bound by the normal complementary DNA. To date, there has been little structural information available on these antisense nucleotides, in spite of ..
  28. Dinitroaniline Herbicide Resistance in Toxoplasma gondii
    MARIA FICHERA; Fiscal Year: 2001
    ..This protocol will be repeated using cDNA (complementary DNA) constructs prepared by RT-PCR (reverse transcription PCR)...
    Hiroshi Takahashi; Fiscal Year: 2000
    ..studies, an effort will be made to determine the protein sequence of purified SF-25 antigen and clone a complementary DNA (cDNA) encoding this tumor-associated antigen...
    Gregory May; Fiscal Year: 1999
    ..We have cloned and sequenced a myosin I complementary DNA (cDNA) from Aspergillus nidulans and have designated its gene myoA...
  31. Relationships between B Cell Antigen Receptor Signaling and Endocytosis
    MARCUS CLARK; Fiscal Year: 2007
    ..cell line in which the endogenous clathrin alleles have been disrupted and replaced with a human clathrin complementary DNA under the control of a tetracycline-regulated promoter...
    Thomas Segerson; Fiscal Year: 1992
    ..To accomplish this aim, a complementary DNA that encodes the receptor for TRH will be cloned, using Xenopus oocyte expression system...
    Charles Boyd; Fiscal Year: 1993
    ..To accomplish this goal we will use two different approaches: (a) We will synthesize complementary DNA (cDNA) to Type IV collagen-specific mRNA isolated from a human tumor cell line (American Type Culture ..
  34. Structural studies of protein-nucleic acid interactions
    Millie Georgiadis; Fiscal Year: 2005
    ..synthesizes DNA using first the single-stranded retroviral RNA as the template and subsequently the complementary DNA strand as a template in order to produce a double-stranded DNA copy of the genome...
    VINCENT YANG; Fiscal Year: 1993
    ..using primarily chromatographic procedures and the other involves the screening of an expression phage complementary DNA (cDNA) library with a labeled oligonucleotide containing EDBP-binding sequences, 2) to isolate cDNA clones ..
    Gayle Lamppa; Fiscal Year: 1993
    ..A gene coding for the enzyme will be isolated from a complementary DNA library and sequenced to study the structure of the polypeptide and its biosynthetic pathway...
    Qiang Li; Fiscal Year: 1993
    ..This test is a necessary step in the operation of the instrument. We will also measure the binding of the complementary DNA to assess the detection limit, sensitivity and the issue of non-specific binding...
    STEVEN SIGNS; Fiscal Year: 1991
    ..colony hybridization" and "subtracted library hybridization" will be used to identify and characterize complementary DNA (cDNA) clones of rat brain mRNA altered (induced or repressed) as a result of ethanol dependence an ..
    Stuart Kupfer; Fiscal Year: 1993
    ..Natural mutations will be recreated by site directed mutagenesis of normal human AR complementary DNA, and the functional characteristics of the expressed receptors will be analyzed for steroid binding, DNA ..
    David Viskochil; Fiscal Year: 1993
    ..b>Complementary DNA sequence will be used to characterize expression of the NF1 gene in neural crest-derived tissue...
  41. Directed MSC differentiation via controlled transfection
    William Murphy; Fiscal Year: 2007
    ..In particular, we plan to exploit well-characterized complementary DNA interactions to controllably sequester plasmid DNA on hydrogel substrates...
  42. Multiplexing Cancer Sample Preparation: Indirect Immunomagnetic Enrichment
    Vamsee Pamula; Fiscal Year: 2009
    ..The codes and anti-codes can simply be two complementary DNA strands...
    Steven Zimmerman; Fiscal Year: 2005
    ..capable of additional hydrogen bonding (beyond Watson-Crick) and base-stacking interactions with the complementary DNA strand...
    TAE OH; Fiscal Year: 1992
    ..Aim B is designed to produce specific antibodies to MDNTF, assess its tissue localization, and clone complementary DNA (cDNA) for MDNTF...
    Gary Matthews; Fiscal Year: 2005
    ..From amino acid sequences derived from complementary DNA encoding CNG channel subtypes, selective antibodies will be produced that can be used to localize the ..
  46. Reagents for Preparing Structure-Free DNA and RNA
    Howard Gamper; Fiscal Year: 2007 from ourselves and others indicate that this goal should be readily attainable and would form the basis for evaluating pseudo-complementary DNA and RNA targets for use in conjunction with short probes in a microarray format.
    Charles Simmons; Fiscal Year: 1991
    ..structure-function relationships of (Na+ and K+) ATPase isoforms, we will isolate and sequence full length complementary DNA (cDNA) clones of the alpha (catalytic) and beta subunits of rat (Na+ and K+) ATPase...
    John David; Fiscal Year: 1980
    ..I will use a complementary DNA copy of purified myosin heavy chain (MHC) mRNA as a highly selective hybridization probe to detect the MHC ..
    RUSHDI ALUL; Fiscal Year: 1993
    ..Interestingly, no binding to complementary DNA was detected suggesting a new class of RNA specific hybridization agents...
    Walter Hill; Fiscal Year: 2003
    ..We have previously used short, complementary DNA oligomers to assess the availability of various regions of ribosomal RNA at different stages of translation...
    Yong Choi; Fiscal Year: 1991
    ..We plan to attack these questions (1) by purifying BCGF to homogeneity; (2) by cloning complementary DNA for BCGF; (3) by characterizing their immunological function; (4) by characterizing biochemically receptors ..
  52. Antibody-mediated Antisense Delivery to Solid Tumors
    Enzo Palma; Fiscal Year: 2005
    ..double stranded oligonucleotide heteroduptex comprising a nuclease-resistant antisense agent and a partially complementary DNA "carrier" strand terminally derivatized with antigenic epitopes for antibody binding...
    Gregory Germino; Fiscal Year: 2002
    ..involves the production of high-density arrays (microarrays) that contain thousands of previously isolated complementary DNA (cDNA) sequences and which are used to analyze gene expression on a global scale...
  54. Affinity Mass Tags for Differential Phenotyping
    Christopher Becker; Fiscal Year: 2002
    ..these uses DNA as a tunable affinity tag that can be exploited in multiplexed capture on nanoparticles with complementary DNA. These are integrated into a Flag tag-based Protein Separation (FLAPS) strategy that may achieve pre-..
    JANET ROBISHAW; Fiscal Year: 1992
    ..The first objective will be to isolate complementary DNA (cDNA) clones for bovine brain gamma subunit mRNAs by oligodeoxyribonucleotide hybridization using probes ..
    JOSEPHINE CLARK CURTISS; Fiscal Year: 1999
    ..the pathogenicity of this organism through molecular and functional analyses of genomic DNA, recombinant molecules from genomic libraries and complementary DNA (cDNA) libraries produced from messenger RNA isolated from M. avium bacilii.
  57. Microfabricated Device for Rapid, Portable, Viral Genome
    RONALD LARSON; Fiscal Year: 2004
    ..device, perform an RT-PCR reaction on HA 1 hemagglutinin domain of influenza A, producing double-stranded complementary DNA. Aim 3 - On a microfabricated device, perform restriction digestion reactions that can distinguish H1, H3, ..
    Masaki Hayashi; Fiscal Year: 1980
    ..We propose to clone tubulin complementary DNA in the plasmid pBR322...
    Ke He Ruan; Fiscal Year: 2008
    ..Determine the solution conformation of segments comprising the intracellular domains. ..
  60. Novel Molecular Profiling of Prostate Cancer Signatures
    Shao Yao Ying; Fiscal Year: 2006
    ..These results may pave the way for a precise gene-chip diagnosis of stage-specific markers of human prostate cancer. ..
  61. Prostaglandin I synthase, Thromboxane A synthase & Prostaglandin E synthase
    Ke He Ruan; Fiscal Year: 2009