single stranded dna

Summary

Summary: A single chain of deoxyribonucleotides that occurs in some bacteria and viruses. It usually exists as a covalently closed circle.

Top Publications

  1. ncbi Mfold web server for nucleic acid folding and hybridization prediction
    Michael Zuker
    Department of Mathematical Sciences, Rensselaer Polytechnic Institute, Troy, NY 12180, USA
    Nucleic Acids Res 31:3406-15. 2003
  2. ncbi Sensing DNA damage through ATRIP recognition of RPA-ssDNA complexes
    Lee Zou
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Science 300:1542-8. 2003
  3. ncbi Human CtIP promotes DNA end resection
    Alessandro A Sartori
    The Wellcome Trust and Cancer Research UK Gurdon Institute, and Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Nature 450:509-14. 2007
  4. ncbi The Mcm2-7 complex has in vitro helicase activity
    Matthew L Bochman
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Mol Cell 31:287-93. 2008
  5. ncbi Enzyme-free nucleic acid logic circuits
    Georg Seelig
    Department of Applied Physics, California Institute of Technology, Pasadena, CA 91125, USA
    Science 314:1585-8. 2006
  6. ncbi Trex1 exonuclease degrades ssDNA to prevent chronic checkpoint activation and autoimmune disease
    Yun gui Yang
    Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, UK
    Cell 131:873-86. 2007
  7. ncbi PML-nuclear bodies accumulate DNA in response to polyomavirus BK and simian virus 40 replication
    Asne Jul-Larsen
    Section of Anatomy and Cell Biology, Department of Biomedicine, University of Bergen, 5009, Norway
    Exp Cell Res 298:58-73. 2004
  8. ncbi Human replication protein A-Rad52-single-stranded DNA complex: stoichiometry and evidence for strand transfer regulation by phosphorylation
    Xiaoyi Deng
    The Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 987696 Nebraska Medical Center, Omaha, Nebraska 68198 7696, USA
    Biochemistry 48:6633-43. 2009
  9. ncbi Multiple mechanisms control chromosome integrity after replication fork uncoupling and restart at irreparable UV lesions
    Massimo Lopes
    Department of Biology, Institute of Cell Biology, ETH Honggerberg, CH 8093 Zurich, Switzerland
    Mol Cell 21:15-27. 2006
  10. ncbi DNA strand exchange promoted by RecA K72R. Two reaction phases with different Mg2+ requirements
    Q Shan
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    J Biol Chem 271:5712-24. 1996

Research Grants

  1. AAV Gene Therapy for AAT deficiency
    RICHARD SAMULSKI; Fiscal Year: 2009
  2. AAV Gene Therapy for AAT deficiency
    RICHARD J contact SAMULSKI; Fiscal Year: 2010
  3. Functional Anergy in B Cells
    LAWRENCE WYSOCKI; Fiscal Year: 2009
  4. ROLE OF TOPOISOMERASES IN DNA REPLICATION
    KENNETH MARIANS; Fiscal Year: 1990
  5. Human Parvovirus B19 Vectors: Mechanism of Transduction
    Arun Srivastava; Fiscal Year: 2004
  6. Human Parvovirus B19 Vectors: Mechanism of Transduction
    Arun Srivastava; Fiscal Year: 2007
  7. Development of a novel whole genome amplification method that mimics nature
    Huimin Kong; Fiscal Year: 2007
  8. Recombination and fork progression in bacteriophage T4
    STEPHEN W contact WHITE; Fiscal Year: 2010
  9. ALTERED MECHANICAL LOADS AND SKELETAL MUSCLE PHENOTYPE
    Richard Tsika; Fiscal Year: 2009
  10. MOLECULAR BASIS OF PARVOVIRUS TARGET CELL SPECIFICITY
    Peter Tattersall; Fiscal Year: 2000

Detail Information

Publications292 found, 100 shown here

  1. ncbi Mfold web server for nucleic acid folding and hybridization prediction
    Michael Zuker
    Department of Mathematical Sciences, Rensselaer Polytechnic Institute, Troy, NY 12180, USA
    Nucleic Acids Res 31:3406-15. 2003
    ..The portal for the mfold web server is http://www.bioinfo.rpi.edu/applications/mfold. This URL will be referred to as 'MFOLDROOT'...
  2. ncbi Sensing DNA damage through ATRIP recognition of RPA-ssDNA complexes
    Lee Zou
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Science 300:1542-8. 2003
    ..Our data suggest that RPA-coated ssDNA is the critical structure at sites of DNA damage that recruits the ATR-ATRIP complex and facilitates its recognition of substrates for phosphorylation and the initiation of checkpoint signaling...
  3. ncbi Human CtIP promotes DNA end resection
    Alessandro A Sartori
    The Wellcome Trust and Cancer Research UK Gurdon Institute, and Department of Zoology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK
    Nature 450:509-14. 2007
    ..These findings establish evolutionarily conserved roles for CtIP-like proteins in controlling DSB resection, checkpoint signalling and homologous recombination...
  4. ncbi The Mcm2-7 complex has in vitro helicase activity
    Matthew L Bochman
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Mol Cell 31:287-93. 2008
    ..Our results show that purified Mcm2-7 acts as a helicase, provides functional evidence of a Mcm2/5 gate, and lays the foundation for future mechanistic studies of this critical factor...
  5. ncbi Enzyme-free nucleic acid logic circuits
    Georg Seelig
    Department of Applied Physics, California Institute of Technology, Pasadena, CA 91125, USA
    Science 314:1585-8. 2006
    ..Biological nucleic acids such as microRNAs can serve as inputs, suggesting applications in biotechnology and bioengineering...
  6. ncbi Trex1 exonuclease degrades ssDNA to prevent chronic checkpoint activation and autoimmune disease
    Yun gui Yang
    Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, UK
    Cell 131:873-86. 2007
    ..Our data indicate that Trex1 acts on a single-stranded DNA polynucleotide species generated from processing of aberrant replication intermediates to attenuate DNA damage checkpoint signaling and prevent pathological immune activation...
  7. ncbi PML-nuclear bodies accumulate DNA in response to polyomavirus BK and simian virus 40 replication
    Asne Jul-Larsen
    Section of Anatomy and Cell Biology, Department of Biomedicine, University of Bergen, 5009, Norway
    Exp Cell Res 298:58-73. 2004
    ..These results indicate a role of PML and PML-NBs in post-replication DNA processing, and suggest that PML-NBs become linked to sites of viral DNA synthesis due to a role of these structures in DNA metabolism...
  8. ncbi Human replication protein A-Rad52-single-stranded DNA complex: stoichiometry and evidence for strand transfer regulation by phosphorylation
    Xiaoyi Deng
    The Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 987696 Nebraska Medical Center, Omaha, Nebraska 68198 7696, USA
    Biochemistry 48:6633-43. 2009
    ..This suggests that RPA phosphorylation may regulate the first steps of DSB repair and is necessary for the mediator function of Rad52...
  9. ncbi Multiple mechanisms control chromosome integrity after replication fork uncoupling and restart at irreparable UV lesions
    Massimo Lopes
    Department of Biology, Institute of Cell Biology, ETH Honggerberg, CH 8093 Zurich, Switzerland
    Mol Cell 21:15-27. 2006
    ....
  10. ncbi DNA strand exchange promoted by RecA K72R. Two reaction phases with different Mg2+ requirements
    Q Shan
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    J Biol Chem 271:5712-24. 1996
    ..The mutant protein also facilitates the autocatalytic cleavage of the LexA repressor, but at a reduced rate...
  11. ncbi Devoted to the lagging strand-the subunit of DNA polymerase III holoenzyme contacts SSB to promote processive elongation and sliding clamp assembly
    Z Kelman
    Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    EMBO J 17:2436-49. 1998
    ..These results establish a role for the psi subunit in contacting SSB, thus enhancing the clamp loading and processivity of synthesis of the holoenzyme, presumably by helping to localize the holoenzyme to sites of SSB-coated ssDNA...
  12. ncbi DNA double-strand break repair: all's well that ends well
    Claire Wyman
    Department of Cell Biology and Genetics and Department of Radiation Oncology, Erasmus MC, 3000 DR Rotterdam, The Netherlands
    Annu Rev Genet 40:363-83. 2006
    ....
  13. ncbi Characterization of the Escherichia coli SSB-113 mutant single-stranded DNA-binding protein. Cloning of the gene, DNA and protein sequence analysis, high pressure liquid chromatography peptide mapping, and DNA-binding studies
    J W Chase
    J Biol Chem 259:805-14. 1984
    ..Williams, K. R., Chase, J. W., and Rupp, W. D. (1981) Proc. Natl. Acad. Sci. U.S.A. 78, 4274-4278). The second correction to the DNA sequence is in the serine 39 codon, previously reported to be TCA and now correctly shown to be TCC...
  14. ncbi Non-hexameric DNA helicases and translocases: mechanisms and regulation
    Timothy M Lohman
    Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 S Euclid Avenue, St Louis, Missouri 63110, USA
    Nat Rev Mol Cell Biol 9:391-401. 2008
    ..For some of these enzymes, self assembly and/or interactions with accessory proteins seem to regulate their translocase and helicase activities...
  15. ncbi Mechanistic basis of 5'-3' translocation in SF1B helicases
    Kayarat Saikrishnan
    Cancer Research UK Clare Hall Laboratories, The London Research Institute, Blanche Lane, South Mimms, Potters Bar, Herts EN63LD, UK
    Cell 137:849-59. 2009
    ....
  16. ncbi Analysis of the DNA substrate specificity of the human BACH1 helicase associated with breast cancer
    Rigu Gupta
    Laboratory of Molecular Gerontology, NIA, National Institutes of Health, Baltimore, Maryland 21224, USA
    J Biol Chem 280:25450-60. 2005
    ....
  17. ncbi The obesity-associated FTO gene encodes a 2-oxoglutarate-dependent nucleic acid demethylase
    Thomas Gerken
    Chemistry Research Laboratory and Oxford Centre for Integrative Systems Biology, University of Oxford, 12 Mansfield Road, Oxford, Oxon OX1 3TA, UK
    Science 318:1469-72. 2007
    ..Studies can now be directed toward determining the physiologically relevant FTO substrate and how nucleic acid methylation status is linked to increased fat mass...
  18. ncbi Differential single-stranded DNA binding properties of the paralogous SsbA and SsbB proteins from Streptococcus pneumoniae
    Diane E Grove
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA
    J Biol Chem 280:11067-73. 2005
    ..These results represent the first analysis of paralogous SSB proteins from any bacterial species and provide a foundation for further investigations into the biological roles of these proteins...
  19. ncbi Crystal structure of a biologically functional form of PriB from Escherichia coli reveals a potential single-stranded DNA-binding site
    Seijiro Shioi
    Department of Immunology, Graduate School of Pharmaceutical Sciences, Kyushu University, 3 1 1 Maidashi, Higashi ku, Fukuoka 812 8582, Japan
    Biochem Biophys Res Commun 326:766-76. 2005
    ..The significance of these results with respect to the functional role of PriB in the assembly of primosome is discussed...
  20. ncbi A postsynaptic role for single-stranded DNA-binding protein in recA protein-promoted DNA strand exchange
    P E Lavery
    Department of Cell, Molecular, and Structural Biology, Northwestern University Medical School, Chicago, Illinois 60611
    J Biol Chem 267:9315-20. 1992
    ....
  21. ncbi Crystal structures of complexes of PcrA DNA helicase with a DNA substrate indicate an inchworm mechanism
    S S Velankar
    Sir William Dunn School of Pathology, University of Oxford, United Kingdom
    Cell 97:75-84. 1999
    ..Taken together, these structures provide evidence against an "active rolling" model for helicase action but are instead consistent with an "inchworm" mechanism...
  22. ncbi Hepatitis C virus NS3 RNA helicase domain with a bound oligonucleotide: the crystal structure provides insights into the mode of unwinding
    J L Kim
    Vertex Pharmaceuticals Incorporated, Cambridge, Massachusetts 02139 4242, USA
    Structure 6:89-100. 1998
    ..The structures of several helicases have been published but the structural details as to how ATP binding and hydrolysis are coupled to RNA unwinding are unknown...
  23. ncbi Structural insights into single-stranded DNA binding and cleavage by F factor TraI
    Saumen Datta
    Department of Biology, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA
    Structure 11:1369-79. 2003
    ..The full positive charge on the Mg(2+) and the architecture of the active site suggest multiple roles for Mg(2+) in DNA cleavage...
  24. ncbi Single-stranded DNA-binding protein of Deinococcus radiodurans: a biophysical characterization
    Gregor Witte
    Department of Biophysical Chemistry, Medical School Hannover Hannover, Germany
    Nucleic Acids Res 33:1662-70. 2005
    ..coli, DraSSB took over the in vivo function of EcoSSB. With DraSSB behaving almost identical to EcoSSB the question remains open as to why dimeric SSB proteins have evolved in the Thermus group of bacteria...
  25. ncbi Sae2 is an endonuclease that processes hairpin DNA cooperatively with the Mre11/Rad50/Xrs2 complex
    Bettina M Lengsfeld
    Department of Molecular Genetics and Microbiology, University of Texas at Austin, Austin, TX 78712, USA
    Mol Cell 28:638-51. 2007
    ....
  26. ncbi Anti-DNA antibodies from autoimmune mice arise by clonal expansion and somatic mutation
    M Shlomchik
    Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111
    J Exp Med 171:265-92. 1990
    ....
  27. ncbi Bacillus subtilis bacteriophage SPP1-encoded gene 34.1 product is a recombination-dependent DNA replication protein
    Maria I Martinez-Jimenez
    Departamento de Biotecnologia Microbiana, Centro Nacional de Biotecnologia, CSIC, Darwin 3, Campus de la Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain
    J Mol Biol 351:1007-19. 2005
    ..1P and G36P in concert might lead to the re-establishment of a unidirectional recombination-dependent replication that accounts for the direction of DNA packaging...
  28. ncbi The beta protein of phage lambda promotes strand exchange
    Z Li
    Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA
    J Mol Biol 276:733-44. 1998
    ..These observations reveal a mechanism by which a protein can drive strand exchange in one direction without using ATP or any other exogenous source of energy...
  29. ncbi PriB stimulates PriA helicase via an interaction with single-stranded DNA
    Chris J Cadman
    School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, United Kingdom
    J Biol Chem 280:39693-700. 2005
    ..However, stimulation of PriA by PriB is not DNA structure-specific, demonstrating that targeting of stalled forks and recombination intermediates during replication restart likely resides with PriA alone...
  30. ncbi Mechanism of interaction between single-stranded DNA binding protein and DNA
    Simone Kunzelmann
    MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Biochemistry 49:843-52. 2010
    ..1 microM at a high ionic strength (200 mM NaCl). Shorter lengths of ssDNA were tested for binding: only when the length is reduced to 20 bases is the affinity significantly reduced...
  31. ncbi Human Rad52-mediated homology search and annealing occurs by continuous interactions between overlapping nucleoprotein complexes
    Eli Rothenberg
    Howard Hughes Medical Institute and Center for the Physics of Living Cells, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    Proc Natl Acad Sci U S A 105:20274-9. 2008
    ..In light of these observations we propose a model for hRad52-mediated DNA annealing where ssDNA release and dsDNA zippering are coordinated through successive rearrangement of overlapping nucleoprotein complexes...
  32. ncbi Crystal structure of human mitochondrial single-stranded DNA binding protein at 2.4 A resolution
    C Yang
    Department of Biochemistry and Biophysics, Washington State University, Pullman 99164 4660, USA
    Nat Struct Biol 4:153-7. 1997
    ..We propose that ssDNA wraps around the tetrameric HsmtSSB protein through electropositive channels guided by flexible loops...
  33. ncbi Physical interaction between replication protein A and Rad51 promotes exchange on single-stranded DNA
    Melissa E Stauffer
    Departments of Biochemistry and Physics and the Center for Structural Biology, Vanderbilt University, Nashville, Tennessee 37232-8725, USA
    J Biol Chem 279:25638-45. 2004
    ..Rates of RPA displacement for the mutant were significantly below those of wild-type Rad51, suggesting that a direct RPA-Rad51 interaction is involved in displacing RPA in the initiation stage of genetic recombination...
  34. ncbi Human Fbh1 helicase contributes to genome maintenance via pro- and anti-recombinase activities
    Kasper Fugger
    Institute of Cancer Biology and Center for Genotoxic Stress Research, Danish Cancer Society, DK 2100 Copenhagen, Denmark
    J Cell Biol 186:655-63. 2009
    ..Thus, by possessing both pro- and anti-recombinogenic potential, hFbh1 may cooperate with other DNA helicases in tightly controlling cellular HR activity...
  35. ncbi Cdc13p: a single-strand telomeric DNA-binding protein with a dual role in yeast telomere maintenance
    C I Nugent
    Department of Molecular and Human Genetics and Cell and Molecular Biology Program, Baylor College of Medicine, Houston, TX 77030, USA
    Science 274:249-52. 1996
    ..In vitro, purified Cdc13p binds to single-strand yeast telomeric DNA. Therefore, Cdc13p is a telomere-binding protein required to protect the telomere and mediate access of telomerase to the chromosomal terminus...
  36. ncbi Fine-structure analysis of activation-induced deaminase accessibility to class switch region R-loops
    Kefei Yu
    Department of Biochemistry and Molecular Biology, USC Norris Cancer Ctr, Rm. 5428, 1441 Eastlake Ave, MC9176, Los Angeles, CA 90033, USA
    Mol Cell Biol 25:1730-6. 2005
    ..This phenomenon may explain the lack of WRC site preference at the mutations surrounding class switch recombination junctions...
  37. ncbi Saccharomyces Ku70, mre11/rad50 and RPA proteins regulate adaptation to G2/M arrest after DNA damage
    S E Lee
    Rosenstiel Center, Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Cell 94:399-409. 1998
    ..We suggest that escape from the DNA damage-induced G2/M checkpoint depends on the extent of ssDNA created at broken chromosome ends. RPA appears to play a key intermediate step in this adaptation...
  38. ncbi Dynamics of RecA filaments on single-stranded DNA
    Marijn T J van Loenhout
    Kavli Institute of Nanoscience, Delft University of Technology, Lorentzweg 1, 2628 CJ Delft, The Netherlands
    Nucleic Acids Res 37:4089-99. 2009
    ..Taken together, our results demonstrate that ATP hydrolysis has a major influence on the structure and state of RecA filaments on ssDNA...
  39. ncbi Three conformational snapshots of the hepatitis C virus NS3 helicase reveal a ratchet translocation mechanism
    Meigang Gu
    Laboratory of Virology and Infectious Disease, Center for the Study of Hepatitis C, The Rockefeller University, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 107:521-8. 2010
    ..These findings suggest feasible strategies for developing specific inhibitors to block the action of this attractive, yet largely unexplored drug target...
  40. ncbi BRCA2 function in DNA binding and recombination from a BRCA2-DSS1-ssDNA structure
    Haijuan Yang
    Department of Pharmacology, Sloan Kettering Division, Joan and Sanford I Weill Graduate School of Medical Sciences, Cornell University, New York, NY 10021, USA
    Science 297:1837-48. 2002
    ..These findings establish that BRCA2 functions directly in homologous recombination and provide a structural and biochemical basis for understanding the loss of recombination-mediated DSB repair in BRCA2-associated cancers...
  41. ncbi The ordered assembly of the phiX174-type primosome. I. Isolation and identification of intermediate protein-DNA complexes
    J Y Ng
    Graduate Program in Molecular Biology, Cornell University Graduate School of Medical Sciences, New York, New York 10021, USA
    J Biol Chem 271:15642-8. 1996
    ..As described in an accompanying article (Ng, J. Y., and Marians, K. J. (1996) J. Biol. Chem. 271, 15649-15655), when assembled on intact phiX174 phage DNA, the primosome also contains PriC...
  42. ncbi DNA length dependence of the single-strand annealing pathway and the role of Saccharomyces cerevisiae RAD59 in double-strand break repair
    N Sugawara
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Mol Cell Biol 20:5300-9. 2000
    ..A msh3 rad59 double mutant was more severely defective in SSA than either single mutant...
  43. ncbi Functional uncoupling of MCM helicase and DNA polymerase activities activates the ATR-dependent checkpoint
    Tony S Byun
    Department of Molecular Pharmacology, Stanford University, Stanford, CA 94305, USA
    Genes Dev 19:1040-52. 2005
    ....
  44. ncbi Human AlkB homolog 1 is a mitochondrial protein that demethylates 3-methylcytosine in DNA and RNA
    Marianne Pedersen Westbye
    Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, N 7489 Trondheim, Norway
    J Biol Chem 283:25046-56. 2008
    ..In conclusion, hABH1 is a functional mitochondrial AlkB homolog that repairs 3-methylcytosine in single-stranded DNA and RNA...
  45. ncbi The structural determinants of checkpoint activation
    Christina A MacDougall
    Department of Chemical and Systems Biology, Stanford University, Stanford, California 94305, USA
    Genes Dev 21:898-903. 2007
    ..These observations define the minimal DNA requirements for checkpoint activation and suggest that primed ssDNA represents a common checkpoint activating-structure formed following many types of damage...
  46. ncbi The single-end invasion: an asymmetric intermediate at the double-strand break to double-holliday junction transition of meiotic recombination
    N Hunter
    Department of Molecular and Cellular Biology, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA
    Cell 106:59-70. 2001
    ..This feature can explain crossover suppression between homeologous and structurally heterozygous chromosomes...
  47. ncbi Solution of a 20-variable 3-SAT problem on a DNA computer
    Ravinderjit S Braich
    University of Southern California, Laboratory for Molecular Science, Los Angeles, CA 90089-1340, USA
    Science 296:499-502. 2002
    ..This computational problem may be the largest yet solved by nonelectronic means. Problems of this size appear to be beyond the normal range of unaided human computation...
  48. ncbi A dimeric mutant of the homotetrameric single-stranded DNA binding protein from Escherichia coli
    Marco Landwehr
    Biophysical Chemistry, Medical School Hannover, Germany
    Biol Chem 383:1325-33. 2002
    A single amino acid substitution (Y78R) at the dimer-dimer interface of homotetrameric single stranded DNA binding protein from E. coli (EcoSSB) renders the protein a stable dimer...
  49. ncbi Thermophoresis of single stranded DNA
    Philipp Reineck
    Department of Physics, Systems Biophysics, Center for Nanoscience, Ludwig Maximilians Universitat Munchen, Munchen, Germany
    Electrophoresis 31:279-86. 2010
    ..Here we measured the thermophoresis of highly diluted single stranded DNA using an all-optical capillary approach. Temperature gradients were created locally by an infrared laser...
  50. ncbi The ordered assembly of the phiX174-type primosome. III. PriB facilitates complex formation between PriA and DnaT
    J Liu
    Graduate Program in Molecular Biology, Cornell University Graduate School of Medical Sciences, New York, New York 10021, USA
    J Biol Chem 271:15656-61. 1996
    ..Thus, during primosome assembly, PriB facilitates complex formation between PriA and DnaT...
  51. ncbi Preferential binding to branched DNA strands and strand-annealing activity of the human Rad51B, Rad51C, Rad51D and Xrcc2 protein complex
    Hiroshi Yokoyama
    RIKEN Genomic Sciences Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan
    Nucleic Acids Res 32:2556-65. 2004
    ..2 kb in length) and its complementary circular ssDNA. These properties of the BCDX2 complex may be important for its roles in the maintenance of chromosomal integrity...
  52. ncbi NMR study on the interaction between RPA and DNA decamer containing cis-syn cyclobutane pyrimidine dimer in the presence of XPA: implication for damage verification and strand-specific dual incision in nucleotide excision repair
    Joon-Hwa Lee
    Department of Chemistry and National Creative Research Initiative Center, Korea Advanced Institute of Science and Technology, 373-1, Guseong-dong, Yuseong-gu, Daejon 305-701, Korea
    Nucleic Acids Res 31:4747-54. 2003
    ..The strand-specific binding of RPA and XPA to the damaged duplex DNA shows that RPA and XPA play crucial roles in damage verification and guiding cleavage of damaged DNA during NER...
  53. ncbi Mechanism of homologous recombination from the RecA-ssDNA/dsDNA structures
    Zhucheng Chen
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 453:489-4. 2008
    ..The underwound, stretched filament conformation probably evolved to destabilize the donor duplex, freeing the complementary strand for homology sampling...
  54. ncbi Structure of Mycobacterium smegmatis single-stranded DNA-binding protein and a comparative study involving homologus SSBs: biological implications of structural plasticity and variability in quaternary association
    K Saikrishnan
    Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560 012, India
    Acta Crystallogr D Biol Crystallogr 61:1140-8. 2005
    ..coli SSB partly on account of the difference in the shape of the tetramers. Another difference between the two modes is that the former contains additional ionic interactions and is more susceptible to salt concentration...
  55. ncbi DNA repair synthesis facilitates RAD52-mediated second-end capture during DSB repair
    Michael J McIlwraith
    Cancer Research UK, London Research Institute, Clare Hall Laboratories, South Mimms, Herts EN6 3LD, UK
    Mol Cell 29:510-6. 2008
    ..coli SSB. Following repair synthesis and second-end capture, de novo DNA synthesis was observed from the captured second DNA end...
  56. ncbi Detecting single stranded DNA with a solid state nanopore
    Daniel Fologea
    Department of Physics, University of Arkansas, Fayetteville, AR 72701, USA
    Nano Lett 5:1905-9. 2005
    Voltage biased solid-state nanopores are used to detect and characterize individual single stranded DNA molecules of fixed micrometer length by operating a nanopore detector at pH values greater than approximately 11.6...
  57. ncbi Toroidal structure of lambda-exonuclease
    R Kovall
    Institute of Molecular Biology, Howard Hughes Medical Institute, and Department of Physics, University of Oregon, Eugene, OR 97403, USA
    Science 277:1824-7. 1997
    ..The results also suggest the locations of the active sites and the constraints that limit cleavage to a single strand...
  58. ncbi RecA protein promotes the regression of stalled replication forks in vitro
    M E Robu
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706 1544, USA
    Proc Natl Acad Sci U S A 98:8211-8. 2001
    ..The reaction is not affected by the inclusion of the RecO and R proteins. We present this reaction as one of several potential RecA protein roles in the repair of stalled and/or collapsed replication forks in bacteria...
  59. ncbi Demethylation of 3-methylthymine in DNA by bacterial and human DNA dioxygenases
    Pertti Koivisto
    Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms, Hertfordshire EN6 3LD, United Kingdom
    J Biol Chem 279:40470-4. 2004
    ..Our data suggest that 3-methylthymine residues in DNA will be repaired inefficiently in vivo and therefore may occur at a low steady-state level, but the residues should not gradually accumulate to high levels in long lived cells...
  60. ncbi SSB protein diffusion on single-stranded DNA stimulates RecA filament formation
    Rahul Roy
    Center for Biophysics and Computational Biology, University of Illinois, Urbana Champaign, Illinois 61801, USA
    Nature 461:1092-7. 2009
    ..This observation of diffusional movement of a protein on ssDNA introduces a new model for how an SSB protein can be redistributed, while remaining tightly bound to ssDNA during recombination and repair processes...
  61. ncbi Fluorescent single-stranded DNA binding protein as a probe for sensitive, real-time assays of helicase activity
    Mark S Dillingham
    DNA Protein Interactions Unit, Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol, United Kingdom
    Biophys J 95:3330-9. 2008
    ..Its use in the study of DNA manipulations involving ssDNA intermediates is demonstrated in assays for DNA unwinding, catalyzed by DNA helicases...
  62. ncbi A new class of homogeneous nucleic acid probes based on specific displacement hybridization
    Qingge Li
    The Key Laboratory of Cell Biology and Tumor Cell Engineering of the Ministry of Education, Xiamen 361005, Fujian, China
    Nucleic Acids Res 30:E5. 2002
    ..This class of probes should find applications in a variety of areas wherever high specificity of nucleic acid hybridization is relevant...
  63. ncbi A hand-off mechanism for primosome assembly in replication restart
    Matthew Lopper
    Department of Biomolecular Chemistry, University of Wisconsin School of Medicine and Public Health, 550 Medical Sciences Center, 1300 University Avenue, Madison, WI 53706, USA
    Mol Cell 26:781-93. 2007
    ..This model helps explain how origin-independent initiation of DNA replication is restricted to repaired replication forks, preventing overreplication of the genome...
  64. ncbi In vitro reconstitution of a single-stranded transposition mechanism of IS608
    Catherine Guynet
    Laboratoire de Microbiologie et Genetique Moleculaires, CNRS UMR5100, 118 Rte de Narbonne, F31062 Toulouse Cedex, France
    Mol Cell 29:302-12. 2008
    ..This single-strand transposition mode has important implications not only for dispersion of IS608 but also for the other members of this very large IS family...
  65. ncbi The far upstream element-binding proteins comprise an ancient family of single-strand DNA-binding transactivators
    T Davis-Smyth
    Laboratory of Pathology, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 271:31679-87. 1996
    ..Comparison with GenBank sequences revealed a fourth FBP family member encoded by Caenorhabditis elegans chromosome III, illustrating the high degree of homology in this evolutionarily ancient and conserved family...
  66. ncbi A comparative analysis of Dmc1 and Rad51 nucleoprotein filaments
    Sean D Sheridan
    Committee on Genetics, University of Chicago, Chicago, IL 60637, USA
    Nucleic Acids Res 36:4057-66. 2008
    ....
  67. ncbi High-resolution AFM imaging of single-stranded DNA-binding (SSB) protein--DNA complexes
    Loic Hamon
    Laboratoire de Structure et Activité des Biomolécules Normales et Pathologiques, INSERM U829, Université d Evry Val d Essonne EA3637, Evry, F 91025, France
    Nucleic Acids Res 35:e58. 2007
    ..In association with conventional biochemical techniques, this work should make it possible to study the dynamics of DNA processes involving DNA-SSB complexes as intermediates by AFM...
  68. ncbi Kinetic regulation of single DNA molecule denaturation by T4 gene 32 protein structural domains
    Kiran Pant
    Department of Physics, Northeastern University, 111 Dana Research Center, Boston, MA 02115, USA
    J Mol Biol 327:571-8. 2003
    ..We also obtain equilibrium measurements of the helix-coil transition free energy in the presence of these proteins for the first time...
  69. ncbi Molecular biology: Disruptive influence
    Marco Foiani
    Nature 423:234-5. 2003
  70. ncbi Molecular biology. Beginning at the end
    Antony M Carr
    Genome Damage and Stability Centre, University of Sussex, Falmer, Sussex BN1 9RQ, UK
    Science 300:1512-3. 2003
  71. ncbi Catalysis of strand annealing by replication protein A derives from its strand melting properties
    Jeremy D Bartos
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY 14642, USA
    J Biol Chem 283:21758-68. 2008
    ....
  72. ncbi Biochemical characterization of the RECQ4 protein, mutated in Rothmund-Thomson syndrome
    Margaret A Macris
    Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, 333 Cedar St, C130 Sterling Hall of Medicine, New Haven, CT 06520 8024, USA
    DNA Repair (Amst) 5:172-80. 2006
    ..Unlike the previously characterized members of the RecQ family, RECQ4 lacks a detectable DNA helicase activity...
  73. ncbi Mismatch repair factor MSH2-MSH3 binds and alters the conformation of branched DNA structures predicted to form during genetic recombination
    Jennifer A Surtees
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853 2703, USA
    J Mol Biol 360:523-36. 2006
    ..Therefore, MSH2-MSH3 binding to its substrates creates a unique nucleoprotein structure that may signal downstream steps in repair that include interactions with MMR and nucleotide excision repair factors...
  74. ncbi Bacteriophage T4 32 protein is required for helicase-dependent leading strand synthesis when the helicase is loaded by the T4 59 helicase-loading protein
    Charles E Jones
    Laboratory of Molecular and Cellular Biology, NIDDK, National Institutes of Health, Building 8, Room 2A19, Bethesda, MD 20892-0830, USA
    J Biol Chem 279:12067-75. 2004
    ....
  75. ncbi Oxidation of 7,8-dihydro-8-oxoguanine affords lesions that are potent sources of replication errors in vivo
    Paul T Henderson
    Division of Bioengineering and Environmental Health and Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
    Biochemistry 41:914-21. 2002
    ..These data establish a model whereby the modestly mutagenic primary lesion 8-oxodG is oxidized in vivo to much more highly mutagenic secondary lesions...
  76. ncbi The phage T4 protein UvsW drives Holliday junction branch migration
    Michael R Webb
    Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA
    J Biol Chem 282:34401-11. 2007
    ..Taken together, these results strongly support a role for UvsW in the branch migration of Holliday junctions that form during T4 recombination, replication, and repair...
  77. ncbi p53 and recombination intermediates: role of tetramerization at DNA junctions in complex formation and exonucleolytic degradation
    Christine Janz
    Universitätsfrauenklinik und Poliklinik, Prittwitzstrasse 43, D 89075 Ulm, Germany
    Oncogene 21:2130-40. 2002
    ..These results suggest that during recombination wild-type p53, as a tetramer, stably binds to strand transfer regions, enabling the protein to exonucleolytically correct heteroduplex intermediates early after strand invasion...
  78. ncbi Bacillus subtilis bacteriophage SPP1 hexameric DNA helicase, G40P, interacts with forked DNA
    Silvia Ayora
    , , C.S.I.C, , Cantoblanco, E-28049 Madrid, Spain
    Nucleic Acids Res 30:2280-9. 2002
    ..Our results show that hexameric G40P DNA helicase encircles the 5' tail, interacts with the duplex DNA at the ss-double-stranded DNA junction and excludes the 3' tail of the forked DNA...
  79. ncbi D-loop formation by Brh2 protein of Ustilago maydis
    Nayef Mazloum
    Department of Microbiology and Immunology, Cornell University Weill Medical College, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 105:524-9. 2008
    ..However, the mutant protein was unable to enhance the Rad51-catalyzed reaction. The results suggest a model in which Brh2 binding to plasmid DNA attracts and helps capture Rad51-coated ssDNA...
  80. ncbi Supramolecular DNA-streptavidin nanocircles with a covalently attached oligonucleotide moiety
    Christof M Niemeyer
    Universitat Dortmund, Fachbereich Chemie, Biologisch Chemische Mikrostrukturtechnik, Otto Hahn Str 6, D 44227 Dortmund
    J Biomol Struct Dyn 20:223-30. 2002
    ..Moreover, we demonstrate that a pair of nanocircles, containing complementary oligonucleotide moieties, can be hybridized to form specific dimers, thereby generating a novel type of supramolecular DNA-protein nanostructures...
  81. ncbi Specific and efficient binding of xeroderma pigmentosum complementation group A to double-strand/single-strand DNA junctions with 3'- and/or 5'-ssDNA branches
    Zhengguan Yang
    Department of Biochemistry and Molecular Biology, James H Quillen College of Medicine, East Tennessee State University, Johnson City, Tennessee 37614, USA
    Biochemistry 45:15921-30. 2006
    ..Since ds-ssDNA junctions are common intermediates in many DNA metabolic pathways, the additional potential role of XPA in cellular processes is discussed...
  82. ncbi Strand-specific loading of DnaB helicase by DnaA to a substrate mimicking unwound oriC
    Christoph Weigel
    Max Planck Institut fur Molekulare Genetik, Berlin, Germany
    Mol Microbiol 46:1149-56. 2002
    ..We assume that in vivo DnaA also loads the first of two helicase complexes - required for the assembly of two replication forks - to the lower strand of oriC during initiation of bidirectional chromosome replication in E. coli...
  83. ncbi Rad52 and Ku bind to different DNA structures produced early in double-strand break repair
    Dejan Ristic
    Department of Cell Biology and Genetics, Erasmus MC, The Netherlands
    Nucleic Acids Res 31:5229-37. 2003
    ..Rather, they interact with different DNA substrates produced early in DNA double-strand break repair...
  84. ncbi The Rad52-Rad59 complex interacts with Rad51 and replication protein A
    Allison P Davis
    Department of Microbiology and Institute of Cancer Research, Columbia University College of Physicians and Surgeons, 701 W 168th St, New York, NY 10032, USA
    DNA Repair (Amst) 2:1127-34. 2003
    ....
  85. ncbi Repair of oxidized bases in DNA bubble structures by human DNA glycosylases NEIL1 and NEIL2
    Hong Dou
    Sealy Center for Molecular Science and Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, Texas 77555, USA
    J Biol Chem 278:49679-84. 2003
    ..OGG1/NTH1-independent repair of oxidized bases in the transcribed sequences supports the possibility that NEILs are preferentially involved in repair of lesions in DNA bubbles generated during transcription and/or replication...
  86. ncbi The BRCA2 homologue Brh2 nucleates RAD51 filament formation at a dsDNA-ssDNA junction
    Haijuan Yang
    Structural Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA
    Nature 433:653-7. 2005
    ..These results establish a BRCA2 function in RAD51-mediated DSB repair and explain the loss of this repair capacity in BRCA2-associated cancers...
  87. ncbi Identification of residues important for DNA binding in the full-length human Rad52 protein
    Janice A Lloyd
    Department of Biochemistry and Molecular Pharmacology, Aaron Lazare Research Building, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605 2324, USA
    J Mol Biol 345:239-49. 2005
    ....
  88. ncbi MutS inhibits RecA-mediated strand exchange with platinated DNA substrates
    Melissa A Calmann
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    Proc Natl Acad Sci U S A 101:14174-9. 2004
    ..MutL addition was without effect even in the presence of MutS. The results suggest that although mismatch repair is beneficial for mutation avoidance, its antirecombination activity on inappropriate substrates can be lethal to the cell...
  89. ncbi The Mre11 complex and the metabolism of chromosome breaks: the importance of communicating and holding things together
    Travis H Stracker
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center and Cornell University Graduate School of Medical Sciences, New York, NY 10021, USA
    DNA Repair (Amst) 3:845-54. 2004
    ..Further genetic analysis of the Mre11 complex will be an invaluable tool for dissecting its function on an organismal level and determining its role in the prevention of malignancy...
  90. ncbi Thermochemistry of protein-DNA interaction studied with temperature-controlled nonequilibrium capillary electrophoresis of equilibrium mixtures
    Maxim Berezovski
    Department of Chemistry, York University, Toronto, Ontario M3J 1P3, Canada
    Anal Chem 77:1526-9. 2005
    ..With its unique features and potential applicability to other macromolecular interactions, temperature-controlled NECEEM establishes a valuable addition to the arsenal of analytical methods used to study dynamic molecular complexes...
  91. ncbi ATM activation by DNA double-strand breaks through the Mre11-Rad50-Nbs1 complex
    Ji Hoon Lee
    Department of Molecular Genetics and Microbiology, Institute of Cellular and Molecular Biology, University of Texas at Austin, 1 University Station, A4800, Austin, TX 78712, USA
    Science 308:551-4. 2005
    ..The unwinding of DNA ends by MRN was essential for ATM stimulation, which is consistent with the central role of single-stranded DNA as an evolutionarily conserved signal for DNA damage...
  92. ncbi The role of homologous recombination repair in the formation of chromosome aberrations
    C S Griffin
    Medical Research Council, Radiation and Genome Stability Unit, Harwell, Oxfordshire, UK
    Cytogenet Genome Res 104:21-7. 2004
    ....
  93. ncbi Cell biology. Guiding ATM to broken DNA
    Robert T Abraham
    Signal Transduction Program, The Burnham Institute, La Jolla, CA 92037, USA
    Science 308:510-1. 2005
  94. ncbi Mechanical measurement of single-molecule binding rates: kinetics of DNA helix-destabilization by T4 gene 32 protein
    Kiran Pant
    Department of Physics, Northeastern University, 111 Dana Research Center, Boston, MA 02115, USA
    J Mol Biol 336:851-70. 2004
    ....
  95. ncbi DNA stimulates Mec1-mediated phosphorylation of replication protein A
    Amy J Bartrand
    Program in Molecular Biology and Human Genetics, Karmanos Cancer Institute, Wayne State University, Detroit, Michigan 48201, USA
    J Biol Chem 279:26762-7. 2004
    ..Our results provide insight into the mechanisms that function in vivo to specifically induce RPA phosphorylation upon initiation of DNA replication, repair, or recombination...
  96. ncbi Hmi1p from Saccharomyces cerevisiae mitochondria is a structure-specific DNA helicase
    Silja Kuusk
    Department of General and Microbial Biochemistry, Institute of Molecular and Cell Biology, University of Tartu, Tartu 51010, Estonia
    J Biol Chem 280:24322-9. 2005
    ..These data indicate that Hmi1p may be targeted to a specific 3'-flap structure, suggesting its possible role in DNA recombination...
  97. ncbi Effect of single-stranded DNA-binding proteins on the helicase and primase activities of the bacteriophage T7 gene 4 protein
    Zheng-Guo He
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 279:22190-7. 2004
    ..Interestingly, wild-type gp2.5 stimulates the unwinding activity of gp4 except at very high concentrations, whereas E. coli SSB protein is highly inhibitory at relative low concentrations...
  98. ncbi Interaction of replication protein A with photoreactive DNA structures
    N A Lebedeva
    Novosibirsk Institute of Bioorganic Chemistry, Siberian Division, Russian Academy of Sciences, Novosibirsk 630090, Russia
    Biochemistry (Mosc) 69:208-15. 2004
    ..The data suggest that RPA should be sensitive to such damages in the double-stranded DNA structure...
  99. ncbi Role of Saccharomyces single-stranded DNA-binding protein RPA in the strand invasion step of double-strand break repair
    Xuan Wang
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts, USA
    PLoS Biol 2:E21. 2004
    ..Thus, RPA appears to play a role in strand invasion as well as in facilitating Rad51 binding to ssDNA, possibly by stabilizing the displaced ssDNA...
  100. ncbi Heteroduplex joint formation by a stoichiometric complex of Rad51 and Rad52 of Saccharomyces cerevisiae
    Naoto Arai
    Department of Applied Biological Science, Nihon University College of Bioresource Sciences, Fujisawa shi, Kanagawa, Japan
    J Biol Chem 280:32218-29. 2005
    ....
  101. ncbi The DNA binding properties of the Escherichia coli RecQ helicase
    Shuo Xing Dou
    Laboratory of Soft Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100080, China
    J Biol Chem 279:6354-63. 2004
    ..5 mM AMPPNP, whereas both AMPPNP and ADP had no detectable effect on double-stranded DNA binding over a large range of nucleotide cofactor concentrations...

Research Grants79

  1. AAV Gene Therapy for AAT deficiency
    RICHARD SAMULSKI; Fiscal Year: 2009
    ..These new variants will be generated using an approach with in vitro "DNA shuffling" and in vivo selection in liver. This study would be very important to design AAV/AAT vector for future human clinical trials. ..
  2. AAV Gene Therapy for AAT deficiency
    RICHARD J contact SAMULSKI; Fiscal Year: 2010
    ..These new variants will be generated using an approach with in vitro "DNA shuffling" and in vivo selection in liver. This study would be very important to design AAV/AAT vector for future human clinical trials. ..
  3. Functional Anergy in B Cells
    LAWRENCE WYSOCKI; Fiscal Year: 2009
    ..Moreover, because lymphocytes also reject transplanted tissue, our discoveries may open a clinical avenue to prevent immune rejection of transplanted tissues and organs. ..
  4. ROLE OF TOPOISOMERASES IN DNA REPLICATION
    KENNETH MARIANS; Fiscal Year: 1990
    ....
  5. Human Parvovirus B19 Vectors: Mechanism of Transduction
    Arun Srivastava; Fiscal Year: 2004
    ..The knowledge gained from these studies will shed light on parvovirus Bl9-host cell interactions, and will lead to further improvements in recombinant parvovirus B19 vectors for their potential use in human gene therapy. ..
  6. Human Parvovirus B19 Vectors: Mechanism of Transduction
    Arun Srivastava; Fiscal Year: 2007
    ..The knowledge gained from these studies will shed light on parvovirus Bl9-host cell interactions, and will lead to further improvements in recombinant parvovirus B19 vectors for their potential use in human gene therapy. ..
  7. Development of a novel whole genome amplification method that mimics nature
    Huimin Kong; Fiscal Year: 2007
    ..At this stage, we believe pWGA is at least equivalent to MDA in terms of amplification bias. The results we would obtain during this Phase I study will help determine if pWGA has less bias when small quantities of template DNA is used. ..
  8. Recombination and fork progression in bacteriophage T4
    STEPHEN W contact WHITE; Fiscal Year: 2010
    ..The project encompasses structural, genetics and biochemical techniques working in tandem to study these important biological questions. ..
  9. ALTERED MECHANICAL LOADS AND SKELETAL MUSCLE PHENOTYPE
    Richard Tsika; Fiscal Year: 2009
    ..abstract_text> ..
  10. MOLECULAR BASIS OF PARVOVIRUS TARGET CELL SPECIFICITY
    Peter Tattersall; Fiscal Year: 2000
    ....
  11. SSDP2 Gene Pathway in Myeloid Neoplasms
    Lalitha Nagarajan; Fiscal Year: 2006
    ..3 locus. Recently the Pl's laboratory has identified a novel gene SSBP2 (sequence specific single stranded DNA binding protein 2), homologous to a chicken gene encoding a protein that binds pyrimidine rich single ..
  12. REGULATION OF DNA REPLICATION
    CHARLES MCHENRY; Fiscal Year: 2000
    ....
  13. Development of a HTS Assay Targeting End Protection of Telomeres
    DEBORAH WUTTKE; Fiscal Year: 2007
    ..Small molecules that specifically target telomeres will provide valuable tools for dissecting the mechanism of telomere function and lay the groundwork for the discovery of cancer therapeutics. ..
  14. MITOCHONDRIAL DNA POLYMERASE--MECHANISM AND STRUCTURE
    LAURIE KAGUNI; Fiscal Year: 2000
    ..To initiate the development of a transgenic fly model to examine the relationship between Pol gamma function, mitochondrial DNA replication fidelity, aging and disease. ..
  15. FUNCTIONS OF AN EIA-ASSOCIATED CELLULAR PROTEIN
    Bayar Thimmapaya; Fiscal Year: 2002
    ..Ad) early promoter (E2-early) which transcribes a region of the viral genome that encodes a 72 kD single stranded DNA binding protein, an 80 kD precursor terminal protein and the 140 kD DNA polymerase all of which are vital ..
  16. Molecular Analysis of Tral IE. Coli Helicase 1) Function
    JOEL SCHILDBACH; Fiscal Year: 2009
    ..The proposed experiments will provide a better understanding of the mechanism of conjugation, eventually allowing manipulations designed to improve human health. ..
  17. Molecular Analysis of Tral IE. Coli Helicase 1) Function
    JOEL SCHILDBACH; Fiscal Year: 2007
    ..The proposed experiments will provide a better understanding of the mechanism of conjugation, eventually allowing manipulations designed to improve human health. ..
  18. Molecular Analysis of Tral IE. Coli Helicase 1) Function
    Joel F Schildbach; Fiscal Year: 2010
    ..The proposed experiments will provide a better understanding of the mechanism of conjugation, eventually allowing manipulations designed to improve human health. ..
  19. Molecular Analysis of Tral IE. Coli Helicase 1) Function
    JOEL SCHILDBACH; Fiscal Year: 2009
    ..The proposed experiments will provide a better understanding of the mechanism of conjugation, eventually allowing manipulations designed to improve human health. ..
  20. Vector and host cellular biology in AAV vector transduction in vivo
    Hiroyuki Nakai; Fiscal Year: 2010
    ..Results of the study will contribute to designing and developing more efficient and safer rAAV vectors and assessing the safety of the vector. ..
  21. ASSEMBLY AND ACTIVATION OF ENZYME-SSDNA COMPLEXES
    Scott W Morrical; Fiscal Year: 2010
    ..Our studies of enzyme-ssDNA assembly mechanisms may therefore shed light on how tumors develop and how infectious diseases progress, and may also suggest new treatment strategies. ..
  22. SSB PROTEINS AS ORGANIZERS OF DNA PRECURSOR BIOSYNTHESIS
    Christopher Mathews; Fiscal Year: 2001
    ..Characterization of the phosphorylation of SSB will explore the role of this modification on protein-protein and protein-DNA associations. ..
  23. Human cytomegalovirus alkaline nuclease (pUL98): potential antiviral target?
    Michael McVoy; Fiscal Year: 2007
    ..Demonstration that these activities are important for viral replication would provide strong justification for future efforts towards discovery of compounds that target this protein's activities. ..
  24. Vector and host cellular biology in AAV vector transduction in vivo
    Hiroyuki Nakai; Fiscal Year: 2007
    ..The proposed project should substantially contribute to building an intellectual foundation for successful human gene therapy. ..
  25. REVERSE TRANSCRIPTASES IN THE PROKARYOTES
    Masayori Inouye; Fiscal Year: 2004
    ....