recombinant dna

Summary

Summary: Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.

Top Publications

  1. ncbi Recombinant human and rat ciliary neurotrophic factors
    P Masiakowski
    Regeneron Pharmaceuticals Inc, Tarrytown, New York
    J Neurochem 57:1003-12. 1991
  2. ncbi Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC
    Mamie Z Li
    Howard Hughes Medical Institute, Department of Genetics, Harvard Partners Center for Genetics and Genomics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, Massachusetts 02115, USA
    Nat Methods 4:251-6. 2007
  3. pmc An efficient recombination system for chromosome engineering in Escherichia coli
    D Yu
    Gene Regulation and Chromosome Biology Laboratory and Mouse Cancer Genetics Program, National Cancer Institute, Division of Basic Science, National Cancer Institute Frederick Cancer Research and Development Center, Frederick, MD 21702, USA
    Proc Natl Acad Sci U S A 97:5978-83. 2000
  4. ncbi A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA
    E C Lee
    Mouse Cancer Genetics Program, National Cancer Institute Frederick, Frederick, Maryland 21702, USA
    Genomics 73:56-65. 2001
  5. ncbi Detection of corn intrinsic and recombinant DNA fragments and Cry1Ab protein in the gastrointestinal contents of pigs fed genetically modified corn Bt11
    E H Chowdhury
    National Institute of Animal Health, Tsukuba, Ibaraki 305 0856, Japan
    J Anim Sci 81:2546-51. 2003
  6. pmc DNA enrichment by allele-specific hybridization (DEASH): a novel method for haplotyping and for detecting low-frequency base substitutional variants and recombinant DNA molecules
    Alec J Jeffreys
    Department of Genetics, University of Leicester, Leicester LE1 7RH, UK
    Genome Res 13:2316-24. 2003
  7. ncbi Recombineering: a powerful new tool for mouse functional genomics
    N G Copeland
    Mouse Cancer Genetic Program, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, Maryland 21702, USA
    Nat Rev Genet 2:769-79. 2001
  8. pmc Enhanced immunogenicity of gp120 protein when combined with recombinant DNA priming to generate antibodies that neutralize the JR-FL primary isolate of human immunodeficiency virus type 1
    Shixia Wang
    Department of Medicine, University of Massachusetts Medical School, 364 Plantation Street, Lazare Research Building, Worcester, MA 01605 2397, USA
    J Virol 79:7933-7. 2005
  9. ncbi Characterization of an associated microfibril protein through recombinant DNA techniques
    S K Horrigan
    Department of Biology, Syracuse University, New York 13244
    J Biol Chem 267:10087-95. 1992
  10. doi Simultaneous detection of recombinant DNA segments introduced into genetically modified crops with multiplex ligase chain reaction coupled with multiplex polymerase chain reaction
    Junichi Mano
    National Food Research Institute, 2 1 12, Kannondai, Tsukuba, Ibaraki 305 8642, Japan
    J Agric Food Chem 57:2640-6. 2009

Research Grants

  1. Molecular Improvement of Bacterial Mosquito Larvicides
    Brian A Federici; Fiscal Year: 2010
  2. ANDROGEN RECEPTOR REGULATION OF REPRODUCTIVE FUNCTION
    Elizabeth Wilson; Fiscal Year: 1992
  3. GENETICALLY ENGINEERED ORAL VACCINES AND CARIES IMMUNITY
    Suzanne Michalek; Fiscal Year: 1993
  4. GENETICALLY ENGINEERED ORAL VACCINES AND CARIES IMMUNITY
    Suzanne Michalek; Fiscal Year: 2000
  5. Cloning and Engineering of Nicking enzymes
    Shuang yong Xu; Fiscal Year: 2004
  6. Non-antiobiotic resistance markers for bacteria
    Herbert Schweizer; Fiscal Year: 2006
  7. DEVELOPMENT OF A LIVE ORAL CHOLERA VACCINE
    JAMES KAPER; Fiscal Year: 1993
  8. MOLECULAR BIOLOGY & FUNCTION OF PLATELET MEMBRANE GP130
    Peter Newman; Fiscal Year: 1990

Detail Information

Publications195 found, 100 shown here

  1. ncbi Recombinant human and rat ciliary neurotrophic factors
    P Masiakowski
    Regeneron Pharmaceuticals Inc, Tarrytown, New York
    J Neurochem 57:1003-12. 1991
    ..The biological activity of both recombinant proteins was comparable to that of native rat CNTF...
  2. ncbi Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC
    Mamie Z Li
    Howard Hughes Medical Institute, Department of Genetics, Harvard Partners Center for Genetics and Genomics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, Massachusetts 02115, USA
    Nat Methods 4:251-6. 2007
    ..SLIC allows efficient and reproducible assembly of recombinant DNA with as many as 5 and 10 fragments simultaneously...
  3. pmc An efficient recombination system for chromosome engineering in Escherichia coli
    D Yu
    Gene Regulation and Chromosome Biology Laboratory and Mouse Cancer Genetics Program, National Cancer Institute, Division of Basic Science, National Cancer Institute Frederick Cancer Research and Development Center, Frederick, MD 21702, USA
    Proc Natl Acad Sci U S A 97:5978-83. 2000
    ..This system will be especially useful for the engineering of large bacterial plasmids such as those from bacterial artificial chromosome libraries...
  4. ncbi A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA
    E C Lee
    Mouse Cancer Genetics Program, National Cancer Institute Frederick, Frederick, Maryland 21702, USA
    Genomics 73:56-65. 2001
    ....
  5. ncbi Detection of corn intrinsic and recombinant DNA fragments and Cry1Ab protein in the gastrointestinal contents of pigs fed genetically modified corn Bt11
    E H Chowdhury
    National Institute of Animal Health, Tsukuba, Ibaraki 305 0856, Japan
    J Anim Sci 81:2546-51. 2003
    ..Genetically modified corn Bt11 is developed by inserting a recombinant DNA sequence encoding insecticidal Cry1Ab protein from Bacillus thuringiensis subsp. kurstaki...
  6. pmc DNA enrichment by allele-specific hybridization (DEASH): a novel method for haplotyping and for detecting low-frequency base substitutional variants and recombinant DNA molecules
    Alec J Jeffreys
    Department of Genetics, University of Leicester, Leicester LE1 7RH, UK
    Genome Res 13:2316-24. 2003
    ..This technique offers a new approach to the analysis of mutation and recombination, and has the potential to detect very rare de novo base substitutions...
  7. ncbi Recombineering: a powerful new tool for mouse functional genomics
    N G Copeland
    Mouse Cancer Genetic Program, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, Maryland 21702, USA
    Nat Rev Genet 2:769-79. 2001
    ....
  8. pmc Enhanced immunogenicity of gp120 protein when combined with recombinant DNA priming to generate antibodies that neutralize the JR-FL primary isolate of human immunodeficiency virus type 1
    Shixia Wang
    Department of Medicine, University of Massachusetts Medical School, 364 Plantation Street, Lazare Research Building, Worcester, MA 01605 2397, USA
    J Virol 79:7933-7. 2005
    ..Here we examined recombinant DNA priming followed by subunit protein boosting as a strategy to generate neutralizing antibodies...
  9. ncbi Characterization of an associated microfibril protein through recombinant DNA techniques
    S K Horrigan
    Department of Biology, Syracuse University, New York 13244
    J Biol Chem 267:10087-95. 1992
    ..primary structure of a new extracellular protein associated with elastic fiber microfibrils was determined by recombinant DNA techniques...
  10. doi Simultaneous detection of recombinant DNA segments introduced into genetically modified crops with multiplex ligase chain reaction coupled with multiplex polymerase chain reaction
    Junichi Mano
    National Food Research Institute, 2 1 12, Kannondai, Tsukuba, Ibaraki 305 8642, Japan
    J Agric Food Chem 57:2640-6. 2009
    ..ligase chain reaction (LCR) (MPCR-MLCR) technique as a novel approach for the simultaneous detection of recombinant DNA segments (e.g., promoters, trait genes, and terminators) of genetically modified (GM) crops...
  11. ncbi Detection and characterization of recombinant DNA expressing vip3A-type insecticidal gene in GMOs--standard single, multiplex and construct-specific PCR assays
    Chandra K Singh
    Industrial Toxicology Research Centre, Post Box No 80, M G Marg, Lucknow, 226001, Uttar Pradesh, India
    Anal Bioanal Chem 390:377-87. 2008
    ..The availability of a reliable PCR assay method prior to commercial release of vip3A-based transgenic crops and food would facilitate rapid and efficient regulatory compliance...
  12. doi Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome
    Daniel G Gibson
    J Craig Venter Institute, Rockville, MD 20850, USA
    Science 319:1215-20. 2008
    ..The methods described here will be generally useful for constructing large DNA molecules from chemically synthesized pieces and also from combinations of natural and synthetic DNA segments...
  13. ncbi Recombinant DNA technologies for construction of precisely designed transgene constructs
    Masato Ohtsuka
    Department of Molecular Life Sciences, Basic Medical Science and Molecular Medicine, School of Medicine, Tokai University, Bohseidai, Isehara, Kanagawa 259 1193, Japan
    Curr Pharm Biotechnol 10:244-51. 2009
    ..Combinational use of these E. coli-based recombinant DNA technologies enables the generation of precisely designed vectors useful for desired genome modification for ..
  14. doi Optimal spliced alignments of short sequence reads
    Fabio De Bona
    Friedrich Miescher Laboratory, Max Planck Society, Spemannstr 39, 72076 Tubingen, Germany
    Bioinformatics 24:i174-80. 2008
    ..This represents a significant challenge given their short length and inherent high error rate...
  15. pmc Transformation and isolation of allelic exchange mutants of Chlamydia psittaci using recombinant DNA introduced by electroporation
    Rachel Binet
    Department of Microbiology and Immunology, F Edward Hebert School of Medicine, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814 4799, USA
    Proc Natl Acad Sci U S A 106:292-7. 2009
    ..Coinheritance of the silent 23S rRNA mutation was seen in 46 of 67 recombinants analyzed, illustrating DNA exchange of up to 1,052 bp in length. These findings provide the first step toward genetic manipulation of Chlamydia...
  16. doi Advances in recombinant DNA technology: corifollitropin alfa, a hybrid molecule with sustained follicle-stimulating activity and reduced injection frequency
    B C J M Fauser
    Department of Reproductive Medicine and Gynecology, University Medical Center, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands
    Hum Reprod Update 15:309-21. 2009
    b>Recombinant DNA technologies have been used to develop longer-acting therapeutic proteins. One approach is to introduce sequences containing additional glycosylation sites...
  17. ncbi Gene therapy for prostate cancer by controlling adenovirus E1a and E4 gene expression with PSES enhancer
    Xiong Li
    Departments of Urology, Microbiology and Immunology, Radiology, Medicine, and Walther Oncology Center, Indiana University School of Medicine, Indianapolis, Indiana
    Cancer Res 65:1941-51. 2005
    ..These data show that adenoviral replication can be tightly controlled in a novel fashion by controlling adenoviral E1a and E4 genes simultaneously with a single enhancer...
  18. ncbi MAGIC, an in vivo genetic method for the rapid construction of recombinant DNA molecules
    Mamie Z Li
    Howard Hughes Medical Institute, Department of Genetics, Harvard Partners Center for Genetics and Genomics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, Massachusetts 02115, USA
    Nat Genet 37:311-9. 2005
    ..method, mating-assisted genetically integrated cloning (MAGIC), that facilitates the rapid construction of recombinant DNA molecules...
  19. ncbi Quantification and persistence of recombinant DNA of Roundup Ready corn and soybean in rotation
    Sylvain Lerat
    Departments of Environmental Biology, Plant Agriculture, and Integrative Biology, University of Guelph, Guelph, Ontario, Canada N1G 2W1
    J Agric Food Chem 55:10226-31. 2007
    ..RR corn and RR soybean cp4 epsps persisted in soil for up to 1 year after seeding. The concentration of recombinant DNA in soil peaked in July and August in RR corn and RR soybean plots, respectively...
  20. doi Real-time polymerase chain reaction monitoring of recombinant DNA entry into soil from decomposing roundup ready leaf biomass
    David J Levy-Booth
    Departments of Environmental Biology, Plant Agriculture, Integrative Biology, and Land Resource Science, University of Guelph, Guelph, ON, N1G 2W1, Canada
    J Agric Food Chem 56:6339-47. 2008
    ..The entry of recombinant DNA into soil from GM crops has been identified as an environmental concern due to the possibility of their ..
  21. ncbi Cancer, viruses, and mass migration: Paul Berg's venture into eukaryotic biology and the advent of recombinant DNA research and technology, 1967-1980
    Doogab Yi
    Program in History of Science, Princeton University, 129 Dickinson Hall, Princeton, NJ 08544 1017, USA
    J Hist Biol 41:589-636. 2008
    The existing literature on the development of recombinant DNA technology and genetic engineering tends to focus on Stanley Cohen and Herbert Boyer's recombinant DNA cloning technology and its commercialization starting in the mid-1970s...
  22. ncbi Application of recombinant DNA methods for production of cholinesterases as organophosphate antidotes and detectors
    Palmer Taylor
    Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, CA 92093 0657, USA
    Arh Hig Rada Toksikol 58:339-45. 2007
    ..novel antidotes for organophosphate poisoning and for detection of the organophosphates, we have turned to recombinant DNA methods to synthesize cholinesterases with unusual properties...
  23. pmc Refactoring bacteriophage T7
    Leon Y Chan
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Mol Syst Biol 1:2005.0018. 2005
    ..The viability of our initial design suggests that the genomes encoding natural biological systems can be systematically redesigned and built anew in service of scientific understanding or human intention...
  24. ncbi Engineering strand-specific DNA nicking enzymes from the type IIS restriction endonucleases BsaI, BsmBI, and BsmAI
    Zhenyu Zhu
    New England Biolabs, Inc 32 Tozer Road, Beverly, MA 01915, USA
    J Mol Biol 337:573-83. 2004
    ..This work provides strong evidence that some type IIS restriction endonucleases carry two separate active sites. When one of the active sites is inactivated, the type IIS restriction endonuclease may nick only one strand...
  25. pmc Roles of fimB and fimE in site-specific DNA inversion associated with phase variation of type 1 fimbriae in Escherichia coli
    M S McClain
    Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109 0620
    J Bacteriol 173:5308-14. 1991
    ..It was found that fimB mediates DNA inversion from on to off, as well as from off to on, and that, contrary to prior interpretations, the fimB-associated inversion occurs with only minimal orientational preference to the on phase...
  26. pmc Marker rescue studies of the transfer of recombinant DNA to Streptococcus gordonii in vitro, in foods and gnotobiotic rats
    Mitra Kharazmi
    Institute of Food Technology, University of Hohenheim, Stuttgart, Germany
    Appl Environ Microbiol 69:6121-7. 2003
    ..It is suggested that the lack of marker rescue is due to the absence of competence-stimulating factors such as serum proteins in rat saliva...
  27. ncbi Phase I Trial of sequential administration of recombinant DNA and adenovirus expressing L523S protein in early stage non-small-cell lung cancer
    John Nemunaitis
    Mary Crowley Medical Research Center, Texas Oncology PA, Dallas, TX 75201, USA
    Mol Ther 13:1185-91. 2006
    ..Results suggest a high level of safety but evidence of L523S-directed immune activation was limited, suggesting a need for modification of dose, schedule, and site of vaccination (i.e., intradermal) with further clinical testing...
  28. ncbi Recombinant DNA technology in the treatment of diabetes: insulin analogs
    Z Vajo
    Section of Endocrinology, VA Medical Center, Phoenix, Arizona 85012, USA
    Endocr Rev 22:706-17. 2001
    After more than half a century of treating diabetics with animal insulins, recombinant DNA technologies and advanced protein chemistry made human insulin preparations available in the early 1980s...
  29. ncbi [Recombineering and its application]
    Jian Guang Zhou
    Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100850, China
    Yi Chuan Xue Bao 30:983-8. 2003
    ..In this article, the bacteriophage lambda Red recombinase system, the progression and applications of this powerful new technique are reviewed according to the data published recently...
  30. ncbi PCR-mediated gene replacement in Escherichia coli
    K C Murphy
    Department of Molecular Genetics and Microbiology University of Massachusetts Medical School, Worcester, MA 01655, USA
    Gene 246:321-30. 2000
    ..coli. Three of these replacements were performed without prior cloning of the genes...
  31. ncbi Expression of active human factor VIII from recombinant DNA clones
    W I Wood
    Nature 312:330-7. 1984
    ..The recombinant protein corrects the clotting time of plasma from haemophiliacs and has many of the biochemical and immunological characteristics of serum-derived factor VIII...
  32. pmc Helper-free stocks of recombinant adeno-associated viruses: normal integration does not require viral gene expression
    R J Samulski
    Department of Biological Sciences, University of Pittsburgh, Pennsylvania 15217
    J Virol 63:3822-8. 1989
    ..Thus, AAV gene expression is not required for normal integration of an infecting DNA containing AAV termini...
  33. pmc Rescue of influenza C virus from recombinant DNA
    Bernadette Crescenzo-Chaigne
    Unite de Genetique Moleculaire des Virus Respiratoires, URA CNRS 1966, EA 302 Université Paris Diderot, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France
    J Virol 81:11282-9. 2007
    ..This efficient system, which does not require helper virus infection, should be useful in viral mutagenesis studies and for generation of expression vectors from type C influenza virus...
  34. pmc Corticotropin and beta-endorphin: construction and analysis of recombinant DNA complementary to mRNA for the common precursor
    J L Roberts
    Proc Natl Acad Sci U S A 76:2153-7. 1979
    ..The data also establish the linkage between beta-melanocyte-stimulating hormone and beta-endorphin as a Lys-Arg sequence. It is hoped that this cloned DNA can be used as a probe to study the expression and structure of the ACTH/LPH gene...
  35. pmc Feasibility of genome-scale construction of promoter::reporter gene fusions for expression in Caenorhabditis elegans using a multisite gateway recombination system
    Ian A Hope
    School of Biology, University of Leeds, Leeds, LS2 9JT, UK
    Genome Res 14:2070-5. 2004
    ..The recombinational cloning involved in the Gateway system, which permits the highly efficient and precise transfer of DNA segments between plasmid vectors, makes this technology ideal for genomics research programs...
  36. ncbi General method for sequence-independent site-directed chimeragenesis
    Kaori Hiraga
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA
    J Mol Biol 330:287-96. 2003
    ..These results illustrate the use of SISDC in creating designed chimeric protein libraries and further illustrate the ability of SCHEMA to identify chimeras whose folded structures are likely not to be disrupted by recombination...
  37. ncbi Plant molecular biology and biotechnology research in the post-recombinant DNA era
    Akhilesh K Tyagi
    Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi 110021, India
    Adv Biochem Eng Biotechnol 84:91-121. 2003
    After the beginning of the recombinant DNA era in the mid-1970s, researchers in India started to make use of the new technology to understand the structure of plant genes and regulation of their expression...
  38. ncbi Remission in models of type 1 diabetes by gene therapy using a single-chain insulin analogue
    H C Lee
    Department of Internal Medicine, Yonsei University, College of Medicine, Seoul, Korea
    Nature 408:483-8. 2000
    ..This new SIA gene therapy may have potential therapeutic value for the cure of autoimmune diabetes in humans...
  39. ncbi Regulated secretion of proinsulin/insulin from human hepatoma cells transduced by recombinant adeno-associated virus
    Y W Yang
    School of Pharmacy, College of Medicine, National Taiwan University, 1, Jen Ai Road, Section 1, Taipei 100, Taiwan
    Biotechnol Appl Biochem 33:133-40. 2001
    ..We conclude that rAAV is a promising vector for gene therapy of diabetes. Regulated secretion of proinsulin/insulin can be obtained in the rAAV-transduced HepG2 cells conferred with the PEPCK promoter via rAAV-mediated gene transfer...
  40. ncbi Purification of recombinant DNA-derived factor IX produced in transgenic pig milk and fractionation of active and inactive subpopulations
    Myles Lindsay
    Department of Chemical Engineering, 133 Randolph Hall, Virginia Tech, Blacksburg, VA 24061, USA
    J Chromatogr A 1026:149-57. 2004
    ..Capillary isoelectric focusing of the active and inactive rFIX fractions demonstrated that the active subpopulations are the most acidic...
  41. ncbi The impact of phage lambda: from restriction to recombineering
    Noreen E Murray
    Institute of Cell Biology, School of Biological Sciences, The University of Edinburgh, The King s Buildings, Mayfield Road, Edinburgh EH9 3JR, UK
    Biochem Soc Trans 34:203-7. 2006
    ..Before recombinant DNA technology, the use of lambda, most particularly lambda transducing phages, illustrated the importance of ..
  42. pmc The Flp double cross system a simple efficient procedure for cloning DNA fragments
    Paul D Sadowski
    Department of Medical Genetics and Microbiology, University of Toronto, Toronto, M5S 1A8 Canada
    BMC Biotechnol 3:9. 2003
    ..Conservative site-specific recombinases offer an efficient alternative to conventional cloning methods...
  43. ncbi Detection of avian infectious bronchitis viral infection using in situ hybridization and recombinant DNA
    E W Collisson
    Department of Veterinary Microbiology and Parasitology, College of Veterinary Medicine, Texas A and M University, College Station 77843 4467
    Vet Microbiol 24:261-71. 1990
    A recombinant DNA probe with specificity for the 3' end of genomic RNA from the Ark 99 strain of infectious bronchitis virus (IBV) was found to hybridize with extracted RNA of three strains with the Ark serotype, as well as the Mass41, ..
  44. ncbi Therapeutic efficacy of hepatitis B surface antigen-antibodies-recombinant DNA composite in HBsAg transgenic mice
    B J Zheng
    Department of Microbiology, The University of Hong Kong, Hong Kong, People's Republic of China
    Vaccine 19:4219-25. 2001
    Therapeutic efficacy of HBsAg-anti-HBs-recombinant DNA harboring hepatitis B virus (HBV) S gene complex was compared with three other therapeutic vaccine candidates (recombinant HBsAg, HBsAg complexed to anti-HBs antibodies and naked ..
  45. ncbi Applications of recombinant DNA technology in gastrointestinal medicine and hepatology: basic paradigms of molecular cell biology. Part A: eukaryotic gene structure and DNA replication
    G E Wild
    Department of Medicine, McGill University Health Centre, Montreal, Quebec, Canada
    Can J Gastroenterol 14:99-110. 2000
    ..Importantly, the application of recombinant DNA technology has played a major role in unravelling the intricacies related to the molecular pathophysiology of ..
  46. pmc Targeted correction of single-base-pair mutations with adeno-associated virus vectors under nonselective conditions
    Xiaoming Liu
    Department of Anatomy and Cell Biology and the Center for Gene Therapy of Cystic Fibrosis and Other Genetic Diseases, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
    J Virol 78:4165-75. 2004
    ..However, the failure of direct cytoplasm- or nucleus-injected rAAV DNA to facilitate gene repair suggests that some aspect of intracellular viral processing may be required to prime recombinant viral genomes for gene repair events...
  47. ncbi Construction and analysis of recombinant DNA for human chorionic somatomammotropin
    J Shine
    Nature 270:494-9. 1977
    ..The primary structure of a major portion of this mRNA species is deduced from the nucleotide sequence of the recombinant DNA.
  48. ncbi Construction of human artificial chromosome vectors by recombineering
    George Kotzamanis
    Division of Biomedical Sciences, Imperial College London, South Kensington, UK
    Gene 351:29-38. 2005
    ..The method can also be used to link any unrelated BAC or PAC insert onto another BAC clone. The EL350 bacteria are an excellent host for building up complex vectors by a combination of homologous and loxP/Cre recombination...
  49. ncbi Improved heterologous gene expression in Trichoderma reesei by cellobiohydrolase I gene (cbh1) promoter optimization
    Ti Liu
    State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China
    Acta Biochim Biophys Sin (Shanghai) 40:158-65. 2008
    ....
  50. pmc A new generation of pPRIG-based retroviral vectors
    Olivier Albagli-Curiel
    INSERM U790 and IFR54, Institut Gustave Roussy, PR1, 39 rue Camille Desmoulins, 94805 Villejuif, France
    BMC Biotechnol 7:85. 2007
    ....
  51. ncbi Strategy to approach stable production of recombinant nattokinase in Bacillus subtilis
    Po Ting Chen
    Department of Chemical Engineering, Feng Chia University, 100 Wenhwa Road, Taichung 40724, Taiwan
    Biotechnol Prog 23:808-13. 2007
    ..Overall, it indicates the feasibility of the approach by fusing two compatible plasmid origins for stable and efficient production of recombinant nattokinase in B. subtilis...
  52. ncbi Properties of the Hansenula polymorpha-derived constitutive GAP promoter, assessed using an HSA reporter gene
    Joo Hyung Heo
    Korea Research Institute of Bioscience and Biotechnology, Yusong P O Box 115, 305 600, Taejon, South Korea
    FEMS Yeast Res 4:175-84. 2003
    ..polymorpha...
  53. ncbi Integration of heterologous genes in several yeast species using vectors containing a Hansenula polymorpha-derived rDNA-targeting element
    Jens Klabunde
    Institut fur Mikrobiologie, Heinrich Heine Universitat Dusseldorf, Universitatsstr 1, 40225, Dusseldorf, Germany
    FEMS Yeast Res 4:185-93. 2003
    ..Additionally, the vectors were examined in two further species, Pichia stipitis and Saccharomyces cerevisiae. Based on these results the design of a 'universal' fungal vector appears to be feasible...
  54. ncbi Collection and characterisation of bacterial membrane proteins
    Massoud Saidijam
    Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, UK
    FEBS Lett 555:170-5. 2003
    ..This strategy for overexpression and purification is extended to additional membrane proteins from H. pylori and from other bacteria...
  55. ncbi Helper-dependent adenoviral vectors mediate therapeutic factor VIII expression for several months with minimal accompanying toxicity in a canine model of severe hemophilia A
    Brian D Brown
    Department of Pathology and Molecular Medicine, Richardson Laboratory, Queen s University, Kingston, ON, Canada K7L 3N6
    Blood 103:804-10. 2004
    ..This study supports previous demonstrations of improved safety using HD gene transfer and suggests that these vectors can provide transient FVIII expression with minimal, acute toxicity in the absence of inhibitor formation...
  56. doi KGF-1 for wound healing in animal models
    Guy P Marti
    Department of Surgery, Johns Hopkins Bayview Medical Center, Baltimore, MD, USA
    Methods Mol Biol 423:383-91. 2008
    ..Considering these results, we believe we can further explore the treatment modalities for using the electroporation-assisted transfection of DNA plasmid expression vectors of growth factors to enhance cutaneous wound healing...
  57. ncbi [Rapid construction of recombinant baculovirus carrying anti-HAV Fab gene using a baculovirus shuttle vector]
    Chang ming Yu
    Institute of Microbiology Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China
    Zhonghua Gan Zang Bing Za Zhi 11:510. 2003
  58. doi Expression and purification of recombinant proteins using the baculovirus system
    Cheryl Isaac Murphy
    Aquila Biopharmaceuticals, Worcester, Massachusetts, USA
    Curr Protoc Mol Biol . 2004
    ....
  59. ncbi Cloning in plasmid vectors
    Carey Pashley
    Department of Medical Microbiology, St Bartholomew s and Royal London School of Medicine and Dentistry, UK
    Methods Mol Biol 235:121-35. 2003
  60. ncbi Escherichia coli host strains
    Nicola Casali
    Division of Infectious Diseases, School of Public Health, University of California, Berkeley, USA
    Methods Mol Biol 235:27-48. 2003
  61. doi Delivery of DNA into bladder via electroporation
    Masaki Yoshida
    Department of Urology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan
    Methods Mol Biol 423:249-57. 2008
    ..These results suggest that EP is a useful technique for in vivo gene transfer into rat bladder smooth muscles, and that the nNOS gene transferred by this procedure functionally expresses and contributes to NO production...
  62. ncbi [Construction of the eukaryotic expression vector PsecTaq2A-AMG for human amelogenin]
    Ailing Yang
    Oral Medicine of West China College of Stomatology, Sichuan University, Chengdu 610041, China
    Hua Xi Kou Qiang Yi Xue Za Zhi 21:133-5. 2003
    ..The purpose of this study was to construct a eukaryotic expression vector for human amelogenin (AMG)...
  63. ncbi Characterization of a recombinant Newcastle disease virus expressing the green fluorescent protein
    Ines Engel-Herbert
    Institute of Molecular Biology, Friedrich Loeffler Institutes, Federal Research Centre for Virus Diseases of Animals, D 17498, Insel Riems, Germany
    J Virol Methods 108:19-28. 2003
    ..Taken together, these data demonstrate the use of GFP-expressing recombinant NDV for analysis of NDV dissemination and pathogenesis and indicate the potential usefulness of NDV as a vaccine vector...
  64. ncbi A new system for analyzing LINE retrotransposition in the chicken DT40 cell line widely used for reverse genetics
    Hiroshi Honda
    Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 B 21 Nagatsuta cho, Midori ku, Yokohama, Kanagawa 226 8501, Japan
    Gene 395:116-24. 2007
    ..Therefore, the DT40 cell system established here provides a powerful tool for the elucidation of LINE retrotransposition pathways, the host factors involved, and their roles...
  65. ncbi Factors influencing therapeutic efficacy and the host immune response to helper-dependent adenoviral gene therapy in hemophilia A mice
    B D Brown
    Department of Pathology and Molecular Medicine, Queen s University, Kingston, Ontario, Canada
    J Thromb Haemost 2:111-8. 2004
    ..Objective: To develop an HD vector capable of mediating sustained FVIII expression and to determine the variables that influence inhibitor development...
  66. pmc Identification of major histocompatibility complex class II-restricted antigens and epitopes of the Epstein-Barr virus by a novel bacterial expression cloning approach
    Slavoljub Milosevic
    Children s Hospital, Technical University Munich, Kolner Platz 1, D 80804 Munich, Germany
    J Virol 80:10357-64. 2006
    ..Using this approach, the BALF4 and BNRF1 proteins were identified as targets of the EBV-specific T-helper-cell response, demonstrating that lytic cycle antigens are a relevant component of the EBV-specific TH-cell response...
  67. ncbi Long-term cardiac gene expression using a coxsackieviral vector
    Byung Kwan Lim
    Department of Medicine, Sungkyunkwan University School of Medicine, Cardiac and Vascular Center, Samsung Medical Center, 50 IL Won Dong, Kangnam Ku, Seoul 135 710, South Korea
    J Mol Cell Cardiol 38:745-51. 2005
    ..These results demonstrated that the cardiac tropism of CVB3 could be used to design vectors for efficient gene expression in the intact heart...
  68. ncbi Introduction of DNA into Actinomycetes by bacterial conjugation from E. coli--an evaluation of various transfer systems
    Franca Blaesing
    Max Planck Institut fur Molekulare Genetik, Ihnestrasse 63 73, D 14195 Berlin, Germany
    J Biotechnol 120:146-61. 2005
    ..The use of plasmids that integrate into the recipients' chromosomes compared to that of plasmids replicating autonomously is shown to allow the access to a wider range of hosts...
  69. ncbi [Construction of LACK gene recombinant plasmid and detection of its expression in eukaryotic cell]
    Ying Ma
    Department of Parasitology, School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China
    Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 21:272-5. 2004
    ..Both RT-PCR and immunofluorescent staining of recombinant plasmid transfected COS-7 showed positive reaction, thus indicating that the recombinant plasmid pcDNA3-LACK can express LACK protein in euka ryotic cell COS-7...
  70. ncbi Production of heterologous proteins in yeast with the aid of the Hsp150delta carrier
    Marja Makarow
    Department of Applied Chemistry and Microbiology, University of Helsinki, Finland
    Methods Mol Biol 313:333-43. 2006
    ..Here we describe the use of the Hsp150delta carrier in P. pastoris in both shake flask and fermentor cultivations. As a reporter protein we use the periplasmic disulfide-bonded Escherichia coli enzyme beta-lactamase...
  71. ncbi Construction of a multiframe vector to express coding sequences in Escherichia coli
    V Dominguez-Martinez
    Facultad de Ciencias Biológicas e Instituto, Salud Pública, Universidad Veracruzana, Xalapa, Ver, Mexico
    Can J Microbiol 47:72-6. 2001
    ..In one case, levels of beta-galactosidase activity depended largely on the N-terminus of the translation products. We conclude that pMFV7 may be useful for expressing coding sequences regardless of their reading frame...
  72. pmc Regulated and liver-specific tamarin alpha interferon gene delivery by a helper-dependent adenoviral vector
    Luigi Aurisicchio
    IRBM Istituto di Ricerche di Biologia Molecolare P Angeletti, Via Pontina Km 30 6, Pomezia, Italy
    J Virol 79:6772-80. 2005
    ..To our knowledge, this is the first report showing a regulated gene expression in a nonhuman primate hepatitis model...
  73. ncbi Transgene stability for three replication-competent murine leukemia virus vectors
    Mogens Duch
    Department of Molecular Biology, University of Aarhus, DK8000, Aarhus, Denmark
    Gene 329:61-9. 2004
    ....
  74. ncbi High-level expression of the 1,3-propanediol oxidoreductase from Klebsiella pneumoniae in Escherichia coli
    Wang Fenghuan
    College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China
    Mol Biotechnol 31:211-9. 2005
    ..The recombinant enzyme was purified and biochemically characterized. The apparent Km values of the enzyme for 1,3-propanediol and NAD+ were 8.5 and 0.21 mM, respectively. The enzyme had maximum activity at pH 9.5 and 30 degrees C...
  75. ncbi Simultaneous sequence transfer into two independent locations of a reporter vector using MultiSite Gateway technology
    Julie Tubb
    University of Washington, Division of Medical Genetics, Seattle 98195 7720, WA
    Biotechniques 39:553-7. 2005
    The bacteriophage lambda recombination system is increasingly used for recombinant DNA applications that involve the frequent transfer of sequences into and between shuttle and reporter vectors...
  76. ncbi Agroinfiltration as a tool for transient expression of cre recombinase in vivo
    Lilya Kopertekh
    Federal Biological Research Centre for Agriculture and Forestry, Institute for Plant Virology, Microbiology and Biosafety, Messeweg 11 12, D 38104 Braunschweig, Germany
    Transgenic Res 14:793-8. 2005
    ..Phenotypic and molecular data indicated that the recombined state has been transferred to the T(1 )generation. These results demonstrate the suitability of agroinfiltration for the expression of cre recombinase in vivo...
  77. ncbi Cloning single-chain antibody fragments (scFv) from hybridoma cells
    Lars Toleikis
    Hospital for Women, University of Heidelberg, Germany
    Methods Mol Med 94:447-58. 2004
    ..Particular emphasis was placed on frequently observed problems and pitfalls of this method...
  78. pmc Nerve growth factor expression by PLG-mediated lipofection
    Kevin J Whittlesey
    Interdepartmental Biological Sciences Program, Northwestern University, Evanston, IL 60208, USA
    Biomaterials 27:2477-86. 2006
    ..Combining this physical support with the ability to locally express neurotrophic factors will potentiate regeneration in nerve injury and disease models...
  79. ncbi Effects of sense tankyrase transfection on smooth muscle cells of corpus cavernosum in rat
    X J Wu
    Urinary Surgery Research Center, Southwest Hospital, Third Military Medical University, Chongqing, PR China
    Arch Androl 52:111-6. 2006
    ..Transfection of recombinant plasmids of sense tankyrase helps change the telomerase length of smooth muscle cells of corpus cavernosum and extend the cell life span...
  80. ncbi Phage phiC31 integrase-mediated genomic integration of the common cytokine receptor gamma chain in human T-cell lines
    Yoshinori Ishikawa
    Department of Microbiology and Immunology, Tohoku University Graduate School of Medicine, 2 1 Seiryo machi, Aoba ku, Sendai 980 8575, Japan
    J Gene Med 8:646-53. 2006
    ..Here, to explore an alternative gene transfer methodology that may offer less risk of insertional mutagenesis, we employed the phiC31 integrase-based integration system using human T-cell lines, including the gammac-deficient ED40515(-)...
  81. pmc Method to integrate multiple plasmids into the mycobacterial chromosome
    Beatrice Saviola
    Basic Medical Sciences, College of Osteopathic Medicine, Western University, 309 E Second Street, Pomona, CA 91766 1854, USA
    Nucleic Acids Res 32:e11. 2004
    ..In particular, this system provides a method by which several recombinant antigens or reporter constructs can be sequentially inserted into a mycobacterial strain and subsequently tested...
  82. ncbi Chimeric adeno-associated virus/antisense U1 small nuclear RNA effectively rescues dystrophin synthesis and muscle function by local treatment of mdx mice
    Michela Alessandra Denti
    Department of Genetics and Molecular Biology, Institute Pasteur Cenci Bolognetti, and IBPM, University La Sapienza, 00185 Rome, Italy
    Hum Gene Ther 17:565-74. 2006
    ..Like U7 snRNA, spliceosomal U1 snRNA is also a suitable backbone for the expression of antisense molecules active in exon skipping...
  83. ncbi [Expression of human leukemia inhibitory factor gene in Pichia pastoris]
    Q Wang
    Biopharmaceutical Center, State Key Laboratory for Biological Control, Zhongshan University, Guangzhou 510275, China
    Wei Sheng Wu Xue Bao 41:582-6. 2001
    ..Density scanning of the SDS-PAGE gel revealed that the targeted protein accounted for 32.8% of the total protein in the supernatants. The expressed products can inhibit the clone formation of murine's teratoma cells...
  84. ncbi [Cloning of trehalose-6-phosphate synthase gene from S. cerevisiae and its plant expression vector construction]
    H Chen
    Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China
    Wei Sheng Wu Xue Bao 41:54-8. 2001
    ..The gene was sequenced and the results showed that the tpsl gene contains 1507 nucleotides and 99.6% identity with S. cerevisiae. The tps1 gene was constructed on the plant expression vector pBin438...
  85. ncbi Efficient expression in Escherichia coli of two species of human interferon-alpha and their hybrid molecules
    J Mizoguchi
    DNA 4:221-32. 1985
    ....
  86. ncbi Construction of expression vectors to produce affinity-tagged proteins in Pseudomonas
    Robin Couch
    Rice University, Houston, TX 77005, USA
    Biotechniques 32:1230, 1232, 1234, 1236. 2002
  87. ncbi Construction of a new Streptococcus pneumoniae-Escherichia coli shuttle vector based on the replicon of an indigenous pneumococcal cryptic plasmid
    R Munoz
    Department of Molecular Microbiology, National Research Center CSIC, Madrid, Spain
    Int Microbiol 2:23-8. 1999
    ..coli for identification of insert-containing plasmids...
  88. ncbi Adenovirus vectors containing chimeric type 5 and type 35 fiber proteins exhibit altered and expanded tropism and increase the size limit of foreign genes
    Hiroyuki Mizuguchi
    Division of Biological Chemistry and Biologicals, National Institute of Health Sciences, 1 18 1 Kamiyoga, Setagaya Ku, 158 8501, Tokyo, Japan
    Gene 285:69-77. 2002
    ....
  89. pmc All six GC-motifs of the SV40 early upstream element contribute to promoter activity in vivo and in vitro
    H Barrera-Saldana
    EMBO J 4:3839-49. 1985
    ....
  90. ncbi Feasibility of biolistic gene therapy in burns
    L Steinstraesser
    Department of Surgery, University of Michigan, 48109-0666, USA
    Shock 15:272-7. 2001
    ....
  91. ncbi Construction of first-generation adenoviral vectors
    Philip Ng
    Departments of Biology and Pathology, McMaster University, Hamilton, Ontario, Canada
    Methods Mol Med 69:389-414. 2002
  92. ncbi Comparison of the homologous carboxy-terminal domain and tail of alpha-crystallin and small heat shock protein
    K B Merck
    Department of Biochemistry, University of Nijmegen, The Netherlands
    Mol Biol Rep 18:209-15. 1993
    ..In the course of this study some differences with the previously published sequence of HSP25 were observed, and a revision is proposed...
  93. ncbi Recombinant plasmid DNA variation of Clostridium oncolyticum--model experiments of cancerostatic gene transfer
    H Schlechte
    , Akademie der Wissenschaften der DDR, Berlin Buch
    Zentralbl Bakteriol Mikrobiol Hyg A 268:347-56. 1988
    ..concolyticum a plasmid-free strain of C. oncolyticum was infected with recombinant DNA. A special microbiological selection system allows the identification of C...
  94. ncbi Nucleotide sequence, structural organization, and functional characterization of the small recombinant plasmid pOM1 that is specific for Francisella tularensis
    A P Pomerantsev
    Department of Microbiology, Kanazawa Medical University, Uchinada, Ishikawa, 920 0293, Japan
    Plasmid 46:86-94. 2001
    ..A 40-kDa protein was encoded by the repA gene and found essential for replication. Expression of the tetC gene is regulated by an Escherichia coli sigma(70)-like promoter and is dependent on the F. tularensis strain and its environment...
  95. ncbi Construction of recombinant vaccinia viruses using PUV-inactivated virus as a helper
    T M Timiryasova
    School of Medicine, Loma Linda University, CA, USA
    Biotechniques 31:534, 536, 538-40. 2001
    ..coli beta-galactosidase. An important feature of these strategies is that any VV strain can be used as a helper virus after PUV inactivation...
  96. ncbi Rapid in vivo isolation of gene expression elements using an HSV amplicon system
    Chun Yuan Huang
    Department of Ophthalmology and Visual Sciences, University of Wisconsin Medical School, 6630 MSC, 1300 University Ave, Madison, WI 53706, USA
    J Virol Methods 113:1-12. 2003
    ..This novel amplicon system should prove valuable in identifying and characterizing GEE for use in gene therapy...
  97. ncbi Characterization of a cryptic plasmid from Lactobacillus fermentum KC5b and its use for constructing a stable Lactobacillus cloning vector
    Sylvia I Pavlova
    Department of Oral Biology, College of Dentistry, University of Illinois at Chicago, 801 South Paulina Street, Chicago, IL 60612, USA
    Plasmid 47:182-92. 2002
    ..As an initial application, pSP1 was used to clone the S-layer protein gene (slpA) of Lactobacillus acidophilus ATCC 4356 into a heterologous vaginal Lactobacillus strain and achieved surface-bound expression of the protein...
  98. ncbi Use of a chimeric adenovirus vector enhances BMP2 production and bone formation
    Elizabeth A Olmsted-Davis
    Center for Cell and Gene Therapy, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA
    Hum Gene Ther 13:1337-47. 2002
    ..Mineralized bone was radiologically identified only in muscle that received the Ad5F35BMP2 transduced hBM-MSCs. In summary, Ad5F35BMP2 can efficiently transduce hBM-MSCs leading to enhanced bone formation in vivo...
  99. pmc Formation of genes coding for hybrid proteins by recombination between related, cloned genes in E. coli
    H Weber
    Nucleic Acids Res 11:5661-9. 1983
    ..Eleven different recombinant genes were identified, six of which encoded new hybrid interferons not easily accessible by recombinant DNA techniques.
  100. ncbi Use of inteins for the in vivo production of stable cyclic peptide libraries in E. coli
    Ernesto Abel-Santos
    Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY, USA
    Methods Mol Biol 205:281-94. 2003
  101. ncbi Construction and expression of a hybrid plasmid containing the Escherichia coli thrA and thrB genes
    P Cossart
    Mol Gen Genet 175:39-44. 1979
    ..The chimeric plasmid was analyzed and characterized genetically, by restriction mapping and DNA sequencing. The limited expression of the threonine biosynthetic enzymes in the strain carrying the recombinant plasmid is discussed...

Research Grants69

  1. Molecular Improvement of Bacterial Mosquito Larvicides
    Brian A Federici; Fiscal Year: 2010
    ..We will use a combination of recombinant DNA technology and analysis of mutants to identify both key proteins and/or regions of the endotoxin proteins ..
  2. ANDROGEN RECEPTOR REGULATION OF REPRODUCTIVE FUNCTION
    Elizabeth Wilson; Fiscal Year: 1992
    ..In this proposal recombinant DNA techniques will be used to complete the isolation and sequencing of androgen receptor cDNA and genomic clones ..
  3. GENETICALLY ENGINEERED ORAL VACCINES AND CARIES IMMUNITY
    Suzanne Michalek; Fiscal Year: 1993
    ..Concurrent with these studies, the recent advancements in recombinant DNA technology and gene cloning have led to additional information on microbial gene products important in ..
  4. GENETICALLY ENGINEERED ORAL VACCINES AND CARIES IMMUNITY
    Suzanne Michalek; Fiscal Year: 2000
    ..Concurrent with these studies, the advancements in recombinant DNA technology and gene cloning have led to additional information on microbial gene products important in ..
  5. Cloning and Engineering of Nicking enzymes
    Shuang yong Xu; Fiscal Year: 2004
    ..of these two enzymes will facilitate application of nicking enzymes in detection of genetic alterations and recombinant DNA technology...
  6. Non-antiobiotic resistance markers for bacteria
    Herbert Schweizer; Fiscal Year: 2006
    ..of antibiotic selection markers, many of which are prohibited for use in these bacteria by NIH Guidelines for Recombinant DNA Research because they potentially compromise the therapeutic use of the respective drugs...
  7. DEVELOPMENT OF A LIVE ORAL CHOLERA VACCINE
    JAMES KAPER; Fiscal Year: 1993
    ..of support, the investigator and his coworkers developed candidate vaccine strains of Vibrio cholerae using recombinant DNA techniques to delete portions of the genes encoding cholera enterotoxin...
  8. MOLECULAR BIOLOGY & FUNCTION OF PLATELET MEMBRANE GP130
    Peter Newman; Fiscal Year: 1990
    ..The recent advent of recombinant DNA technology, however, has made it possible to study many other previously uncharacterized and potentially very ..