Genomes and Genes
Summary: Deoxyribonucleic acid that makes up the genetic material of protozoa.
Publications302 found, 100 shown here
- A large focus of naturally acquired Plasmodium knowlesi infections in human beingsBalbir Singh
Faculty of Medicine and Health Sciences, Universiti Malaysia Sarawak, Kuching, Sarawak, Malaysia
Lancet 363:1017-24. 2004..We aimed to investigate whether such infections could be attributable to a variant form of P malariae or a newly emergent Plasmodium species...
- Genome sequence of the human malaria parasite Plasmodium falciparumMalcolm J Gardner
The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, Maryland 20850, USA
Nature 419:498-511. 2002..The genome sequence provides the foundation for future studies of this organism, and is being exploited in the search for new drugs and vaccines to fight malaria...
- Molecular cloning and cellular localization of a BiP homologue in Trypanosoma brucei. Divergent ER retention signals in a lower eukaryoteJ D Bangs
Department of Microbiology and Immunology, Stanford School of Medicine, CA 94305 5402
J Cell Sci 105:1101-13. 1993..1989) Biochemistry 28, 6440-6446), indicates that endoplasmic reticulum retrieval signals in trypanosomes may be as divergent and heterogeneous as any seen in the other eukaryotes yet studied...
- Detection of four Plasmodium species in blood from humans by 18S rRNA gene subunit-based and species-specific real-time PCR assaysMathieu Rougemont
Institute of Microbiology, University Hospital of Lausanne, Switzerland
J Clin Microbiol 42:5636-43. 2004..This suggests a role of quantitative PCR for the monitoring of patients receiving antimalaria therapy...
- A plastid of probable green algal origin in Apicomplexan parasitesS Kohler
Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA
Science 275:1485-9. 1997..Taken together, these observations indicate that the Apicomplexa acquired a plastid by secondary endosymbiosis, probably from a green alga...
- The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas diseaseNajib M El-Sayed
Department of Parasite Genomics, Institute for Genomic Research, Rockville, MD 20850, USA
Science 309:409-15. 2005....
- Draft genome sequence of the sexually transmitted pathogen Trichomonas vaginalisJane M Carlton
Institute for Genomic Research, 9712 Medical Research Drive, Rockville, MD 20850, USA
Science 315:207-12. 2007..The genome sequence predicts previously unknown functions for the hydrogenosome, which support a common evolutionary origin of this unusual organelle with mitochondria...
- Genetic heterogeneity at the beta-giardin locus among human and animal isolates of Giardiaduodenalis and identification of potentially zoonotic subgenotypesMarco Lalle
Division of Gastroenteric and Tissue Parasitic Diseases, Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanita, Viale Regina Elena, 299, 00161 Rome, Italy
Int J Parasitol 35:207-13. 2005..Five of these subgenotypes, namely A1, A2, A3, A4 and B3, were found to be associated with infections of humans, of dogs and of calves; these data, therefore, supported the role of these animals as a source of infection for humans...
- Genome sequence of Babesia bovis and comparative analysis of apicomplexan hemoprotozoaKelly A Brayton
Program in Genomics, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA
PLoS Pathog 3:1401-13. 2007..The genome sequence provides a greater understanding of B. bovis metabolism and potential avenues for drug therapies and vaccine development...
- Discrimination of all genotypes of Giardia duodenalis at the glutamate dehydrogenase locus using PCR-RFLPCarolyn M Read
Department of Veterinary and Biomedical Sciences, Western Australian Biomedical Research Institute, World Health Collaborating Centre for the Molecular Epidemiology of Parasitic Infections, Murdoch University, Murdoch, WA 6150, Australia
Infect Genet Evol 4:125-30. 2004..duodenalis isolates from human-beings, their companion animals and livestock to be genotyped directly from faeces, leading to valuable information about Giardia genotypes in population without the need for in vitro/in vivo amplification...
- Phylogenetic analyses of diplomonad genes reveal frequent lateral gene transfers affecting eukaryotesJan O Andersson
The Canadian Institute for Advanced Research, Program in Evolutionary Biology, Department of Biochemistry, Dalhousie University, B3H 4H7, Halifax, Nova Scotia, Canada
Curr Biol 13:94-104. 2003..Here, we perform a systematic analysis to investigate the impact of LGT on the evolution of diplomonads, a group of anaerobic protists...
- Detection and identification of Leishmania DNA within naturally infected sand flies by seminested PCR on minicircle kinetoplastic DNAA M Aransay
Laboratory of Clinical Bacteriology, Parasitology, Zoonoses, and Geographical Medicine WHO Collaborating Centre for Research and Training in Mediterranean Zoonoses, Faculty of Medicine, University of Crete, Heraklion, Greece
Appl Environ Microbiol 66:1933-8. 2000..Comparison of the sequences obtained from sand flies and mammal hosts will be crucial for developing hypotheses about the transmission cycles of Leishmania spp. in areas of endemicity...
- Defining the cell cycle for the tachyzoite stage of Toxoplasma gondiiJ R Radke
Department of Veterinary Molecular Biology, Marsh Laboratory, Montana State University, 59717-3610, Bozeman, MT, USA
Mol Biochem Parasitol 115:165-75. 2001..In lieu of a short or missing G2, where checkpoints are thought to operate in other eukaryotes, the bimodal replication of tachyzoite chromosomes may represent a distinct premitotic checkpoint associated with endodyogeny...
- Methylation of histone h3 at lysine 9 targets programmed DNA elimination in tetrahymenaSean D Taverna
Department of Biochemistry and Molecular Genetics, University of Virginia Health System, Charlottesville, VA 22908, USA
Cell 110:701-11. 2002..These results extend the range of H3(Lys9)Me involvement in chromatin activities outside transcriptional regulation and also strengthen the link between heterochromatin formation and programmed DNA elimination...
- Analysis of a piwi-related gene implicates small RNAs in genome rearrangement in tetrahymenaKazufumi Mochizuki
Department of Biology, University of Rochester, Rochester, NY 14627, USA
Cell 110:689-99. 2002..We propose that these small RNAs function to specify sequences to be eliminated by a mechanism similar to RNA-mediated gene silencing...
- Identification of 5 types of Cryptosporidium parasites in children in Lima, PeruL Xiao
Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30341 3717, USA
J Infect Dis 183:492-7. 2001..Contrary to common belief, novel Cryptosporidium species and C. parvum genotypes can infect HIV-negative children...
- Fast and reliable extraction of protozoan parasite DNA from fecal specimensA J da Silva
Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U S Department of Health and Human Services, Atlanta, Georgia 30341 3724, USA
Mol Diagn 4:57-64. 1999..In this study we describe a novel method for DNA extraction from such specimens containing spores and oocysts of Enterocytozoon bieneusi and Cryptosporidium parvum, respectively...
- PCR diagnosis and characterization of Leishmania in local and imported clinical samplesGabriele Schonian
Institute of Microbiology and Hygiene, Humboldt University, Charite, Berlin, Germany
Diagn Microbiol Infect Dis 47:349-58. 2003..113 cases (69.7%) were positive by PCR and species identification was possible in 110 samples. Leishmania DNA was also amplified and identified at the species level from archived non-stained and Giemsa stained microscope slides...
- Association of severe noncerebral Plasmodium falciparum malaria in Brazil with expressed PfEMP1 DBL1 alpha sequences lacking cysteine residuesKarin Kirchgatter
Departmento de Parasitologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Brazil
Mol Med 8:16-23. 2002..We analyzed parasites of Brazilian patients with severe malaria to determine whether there were particular DBL1 alpha var sequences predominantly expressed in such patients...
- The malaria parasite Plasmodium falciparum has only one pyruvate dehydrogenase complex, which is located in the apicoplastBernardo J Foth
Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Parkville, VIC 3010, Australia
Mol Microbiol 55:39-53. 2005..The recombinantly expressed catalytic domain of the PDH subunit E2 showed high enzymatic activity in vitro indicating that pyruvate is converted to acetyl-CoA in the apicoplast, possibly for use in fatty acid biosynthesis...
- Development of a real-time PCR assay for Trypanosoma cruzi detection in blood samplesMaria Piron
Transfusion Safety Laboratory, Banc de Sang i Teixits, Barcelona, Spain
Acta Trop 103:195-200. 2007..Concordance of results with the N-PCR was 90%. In conclusion, real-time PCR provides an optimal alternative to N-PCR, with similar sensitivity and higher throughput, and could help determine ongoing parasitaemia in chagasic patients...
- Establishment of the 1st World Health Organization International Standard for Plasmodium falciparum DNA for nucleic acid amplification technique (NAT)-based assaysDavid J Padley
National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Hertfordshire EN6 3QG, UK
Malar J 7:139. 2008....
- Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas diseaseJuan M Burgos
Laboratorio de Biología Molecular de la Enfermedad de Chagas LaBMECh, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular INGEBI CONICET, Buenos Aires, Argentina
Int J Parasitol 37:1319-27. 2007..Moreover, allelic size polymorphism analysis of microsatellite loci from populations transmitted to twins showed that all clones from the maternal polyclonal population were equally infective to both siblings...
- Alternative polymerase chain reaction method to identify Plasmodium species in human blood samples: the semi-nested multiplex malaria PCR (SnM-PCR)J M Rubio
Animal Taxonomy Department, Wageningen Agricultural University, Wageningen, The Netherlands
Trans R Soc Trop Med Hyg 96:S199-204. 2002....
- Comparison of PCR-RFLP and Genescan-based genotyping for analyzing infection dynamics of Plasmodium falciparumNicole Falk
Swiss Tropical Institute, Basel, Switzerland
Am J Trop Med Hyg 74:944-50. 2006..This reflects the good performance of GeneScan-based genotyping for studies of parasite infection dynamics...
- Leishmania DNA load and cytokine expression levels in asymptomatic naturally infected dogsLaura Manna
Dipartimento di Scienze Cliniche Veterinarie, Universita di Napoli Federico II, Via F Delpino, N 1, 80137 Napoli, Italy
Vet Parasitol 142:271-80. 2006....
- The use of ITS1 rDNA PCR in detecting pathogenic African trypanosomesZ K Njiru
Division of Veterinary and Biomedical Sciences, Western Australian Biomedical Research Institute WABRI, Murdoch University, South Street, 6150, Murdoch, Western Australia, Australia
Parasitol Res 95:186-92. 2005..congolense savannah 30/33 (90.9%) but was lower with T. vivax 25/31 (77.4%). The primers offer promise as a routine diagnostic tool through the use of a single PCR; however, further evaluation is recommended...
- High prevalence of Trypanosoma brucei gambiense group 1 in pigs from the Fontem sleeping sickness focus in CameroonG Simo
Institut de Recherches Médicales et d Etude de Plantes Médicinales IMPM MINRESI, Yaounde, Cameroon
Vet Parasitol 139:57-66. 2006..No significant difference was observed between the percentage of T. b. gambiense group 1 single and mixed infections, and between the prevalence of this trypanosome in pigs from villages with and without sleeping sickness patients...
- Mitosomes of Entamoeba histolytica are abundant mitochondrion-related remnant organelles that lack a detectable organellar genomeGloria León-Avila
School of Biological Sciences, Royal Holloway, University of London, Egham TW20 0EX, UK
Microbiology 150:1245-50. 2004..These results indicate that a remnant organellar genome has not been retained in E. histolytica mitosomes and demonstrate unequivocally that EhKOs and mitosomes are distinct and unrelated cellular structures...
- Wide distribution of Plasmodium ovale in MyanmarT T Win
Department of International Health, Nagoya University School of Medicine, Nagoya, Japan
Trop Med Int Health 7:231-9. 2002..Plasmodium ovale infections were found in villages situated in the southern, central and western regions of Myanmar, suggesting that P. ovale may be widely distributed in this country...
- The zoonotic transmission of Giardia and CryptosporidiumPaul R Hunter
School of Medicine, Health Policy and Practice, University of East Anglia, Norwich NR4 7TJ, UK
Int J Parasitol 35:1181-90. 2005..However, for both parasites there is a need for molecular epidemiological studies to be undertaken in well-defined foci of transmission in order to fully determine the frequency and importance of zoonotic transmission...
- Molecular mimicry of a CCR5 binding-domain in the microbial activation of dendritic cellsJulio Aliberti
Immunobiology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Nat Immunol 4:485-90. 2003..The ability to generate this strong protective response may benefit parasite transmission by preventing the protozoan from overwhelming its intermediate hosts...
- Differential detection of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii by a single-round PCR assayZulhainan Hamzah
Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, 420 6 Rajvithi Road, Bangkok 10400, Thailand
J Clin Microbiol 44:3196-200. 2006....
- Base J: discovery, biosynthesis, and possible functionsPiet Borst
Center of Biomedical Genetics, Division of Molecular Biology, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands
Annu Rev Microbiol 62:235-51. 2008....
- Wild fauna as a probable animal reservoir for Trypanosoma brucei gambiense in CameroonF Njiokou
LRT, OCEAC, BP 288, Yaounde, Cameroon
Infect Genet Evol 6:147-53. 2006..The epidemiological implications of these findings remain to be determined with further investigations...
- Antigenic variation in Babesia bovis occurs through segmental gene conversion of the ves multigene family, within a bidirectional locus of active transcriptionBasima Al-Khedery
Department of Pathobiology, University of Florida, Gainesville, 32611, USA
Mol Microbiol 59:402-14. 2006..These results also suggest the possibility of epigenetic regulation through in situ switching among gene loci, further expanding the potential repertoire of variant proteins...
- Comparison of various sample preparation methods for PCR diagnosis of visceral leishmaniasis using peripheral bloodL Lachaud
, Centre Hospitalier Universitaire, , Montpellier, France
J Clin Microbiol 39:613-7. 2001....
- Transmission studies of Babesia microti in Ixodes ricinus ticks and gerbilsJeremy Gray
Department of Environmental Resource Management, Faculty of Agriculture, Belfield, Dublin 4, Ireland
J Clin Microbiol 40:1259-63. 2002..ricinus suggests that other European B. microti strains, in addition to the HK strain used here, are probably infective for I. ricinus, supporting the view that infection of humans with European B. microti may be a regular occurrence...
- Distinct transmission cycles of Leishmania tropica in 2 adjacent foci, Northern IsraelMilena Svobodova
Charles University, Prague, Czech Republic
Emerg Infect Dis 12:1860-8. 2006..The 2 foci differ with respect to parasites and vectors, but increasing peridomestic rock hyrax populations are probably responsible for emergence of cutaneous leishmaniasis in both foci...
- Sensitive and inexpensive molecular test for falciparum malaria: detecting Plasmodium falciparum DNA directly from heat-treated blood by loop-mediated isothermal amplificationLeo L M Poon
Department of Microbiology, The University of Hong Kong, Hong Kong SAR
Clin Chem 52:303-6. 2006..The aim of the present work was to develop a simple, inexpensive molecular test for Plasmodium falciparum...
- Dinoflagellate expressed sequence tag data indicate massive transfer of chloroplast genes to the nuclear genomeTsvetan R Bachvaroff
Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA
Protist 155:65-78. 2004..A preliminary report of these data was presented at the Botany 2002 meeting in Madison, WI...
- Simultaneous detection of Entamoeba histolytica, Giardia lamblia, and Cryptosporidium parvum in fecal samples by using multiplex real-time PCRJaco J Verweij
Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands
J Clin Microbiol 42:1220-3. 2004..The use of this assay in a diagnostic laboratory would provide sensitive and specific diagnosis of the main parasitic diarrheal infections and could improve patient management and infection control...
- Autochthonous transmission of Trypanosoma cruzi, LouisianaPatricia L Dorn
Department of Biological Sciences, Loyola University New Orleans, New Orleans, Louisiana 70118, USA
Emerg Infect Dis 13:605-7. 2007..The patient had positive test results from 2 serologic tests and hemoculture. Fifty-six percent of 18 Triatoma sanguisuga collected from the house of the patient were positive for T. cruzi by PCR...
- Risk progression to chronic Chagas cardiomyopathy: influence of male sex and of parasitaemia detected by polymerase chain reactionA L Basquiera
Internal Medicine Training Programme, Department of Education, Hospital Privado Centro Medico de Cordoba, Cordoba, Argentina
Heart 89:1186-90. 2003..Polymerase chain reaction (PCR) allows detection of Trypanosoma cruzi in blood throughout the course of Chagas' disease...
- Detection and identification of human Plasmodium species with real-time quantitative nucleic acid sequence-based amplificationPetra F Mens
Koninklijk Instituut voor de Tropen Royal Tropical Institute, KIT Biomedical Research, Meibergdreef 39, 1105 AZ Amsterdam, The Netherlands
Malar J 5:80. 2006..Rapid diagnostic tests based on Plasmodium antigen detection do often not allow for species discrimination as microscopy does, but also become insensitive at <100 parasites/microl...
- Timing of developmentally programmed excision and circularization of Paramecium internal eliminated sequencesM Betermier
UMR 8541 Centre National de la Recherche Scientifique, Laboratoire de Genetique Moleculaire, Ecole Normale Superieure, 75005 Paris, France
Mol Cell Biol 20:1553-61. 2000..The similar structures of the junctions formed on the excised and donor molecules point to a central role for this dinucleotide in IES excision...
- Reference values for Leishmania infantum parasitemia in different clinical presentations: quantitative polymerase chain reaction for therapeutic monitoring and patient follow-upCharles Mary
Laboratoire de Parasitologie, Hopital de la Timone, Marseille, France
Am J Trop Med Hyg 75:858-63. 2006..Its main applications are monitoring of drug therapy and follow-up of immunodeficient patients for biologic confirmation of relapses...
- Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculiM D Katinka
Genoscope, UMR CNRS 8030, CP 5706, 91057 Evry Cedex, France
Nature 414:450-3. 2001..Because the E. cuniculi genome contains genes related to some mitochondrial functions (for example, Fe-S cluster assembly), we hypothesize that microsporidia have retained a mitochondrion-derived organelle...
- DNA G-quartets in a 1.86 A resolution structure of an Oxytricha nova telomeric protein-DNA complexM P Horvath
Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309 0215, USA
J Mol Biol 310:367-77. 2001....
- Placental pathology associated with fetal death in cattle inoculated with Neospora caninum by two different routes in early pregnancyC Macaldowie
Division of Virology, Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian EH26 OPZ, UK
J Comp Pathol 131:142-56. 2004..Of the two inoculation routes, the intravenous induced the more acute placental lesions and greater mortality...
- Plasmodium knowlesi from archival blood films: further evidence that human infections are widely distributed and not newly emergent in Malaysian BorneoKim Sung Lee
Malaria Research Centre, Faculty of Medicine and Health Sciences, Unversiti Malaysia Sarawak, 93150 Kuching, Sarawak, Malaysian Borneo, Malaysia
Int J Parasitol 39:1125-8. 2009..knowlesi in Sarawak and its past occurrence. Taken together with data from previous studies, our findings suggest that P. knowlesi malaria is not a newly emergent disease in humans...
- Outbreak of cutaneous leishmaniasis in northern IsraelRaymond L Jacobson
Department of Parasitology, The Kuvin Center for Study of Infectious and Tropical Diseases, The Hebrew University Hadassah Medical School, Jerusalem, Israel
J Infect Dis 188:1065-73. 2003..tropica foci. P. arabicus is the main vector, and it transmits parasites that are different from other L. tropica isolates, with respect to antigenic, molecular, and biochemical parameters...
- Increasing prevalence of wildtypes in the dihydrofolate reductase gene of Plasmodium falciparum in an area with high levels of sulfadoxine/pyrimethamine resistance after introduction of treated bed netsMichael Alifrangis
Centre for Medical Parasitology, Institute of Medical Microbiology and Immunology, University of Copenhagen, Denmark
Am J Trop Med Hyg 69:238-43. 2003..The impact of ITNs on the transmission intensity seems not only to affect the overall malaria morbidity, but may even facilitate restoration of susceptibility to antimalarial drugs...
- Optimization and validation of multi-coloured capillary electrophoresis for genotyping of Plasmodium falciparum merozoite surface proteins (msp1 and 2)Anne Liljander
Department of Medicine Solna, Karolinska Institutet, SE 171 76 Stockholm, Sweden
Malar J 8:78. 2009..falciparum msp1 and msp2 has been adapted to the CE technique. The protocol and optimization process as well as the potentials and limitations of the technique in molecular epidemiology studies and anti-malarial drug trials are reported...
- Comparison of real-time PCR protocols for differential laboratory diagnosis of amebiasisYvonne Qvarnstrom
National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U S Department of Health and Human Services, Atlanta, Georgia 30341, USA
J Clin Microbiol 43:5491-7. 2005..These results illustrate the usefulness of comparative evaluations of diagnostic assays...
- Two pathways of homologous recombination in Trypanosoma bruceiColin Conway
The Wellcome Centre for Molecular Parasitology, The Anderson College, University of Glasgow, UK
Mol Microbiol 45:1687-700. 2002..We show that the RAD51-independent pathway is capable of recombining DNA substrates with very short lengths of sequence homology and in some cases aberrant recombination reactions can be detected using such microhomologies...
- A novel nested multiplex polymerase chain reaction (PCR) assay for differential detection of Entamoeba histolytica, E. moshkovskii and E. dispar DNA in stool samplesKrishna Khairnar
Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India
BMC Microbiol 7:47. 2007..We have developed a nested multiplex PCR targeting a 16S-like rRNA gene for differential detection of all the three morphologically similar forms of E. histolytica, E. moshkovskii and E. dispar simultaneously in stool samples...
- Evaluation of a real-time polymerase chain reaction assay for the diagnosis of malaria in patients from ThailandHeather Swan
Division of Clinical Microbiology, Mayo Clinic, Rochester, Minnesota 55905, USA
Am J Trop Med Hyg 73:850-4. 2005..It may have application in clinical laboratories, as well as in epidemiologic studies and antimalarial efficacy trials...
- Cryptosporidiosis: an update in molecular epidemiologyLihua Xiao
Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30341, USA
Curr Opin Infect Dis 17:483-90. 2004..These tools have been increasingly used in the characterization of the transmission of Cryptosporidium spp. This review addresses the most recent developments in molecular epidemiology of cryptosporidiosis...
- Host switch leads to emergence of Plasmodium vivax malaria in humansJianbing Mu
Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland, USA
Mol Biol Evol 22:1686-93. 2005..Taken together, a haplotype network, parasite migration patterns, demographic history, and cophylogeny mapping support an Asian origin via a host switch from macaque monkeys...
- JBP1 and JBP2 are two distinct thymidine hydroxylases involved in J biosynthesis in genomic DNA of African trypanosomesLaura J Cliffe
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602, USA
Nucleic Acids Res 37:1452-62. 2009..Reintroduction of JBP2 in the J-null trypanosome stimulates HOMedU formation and site-specific synthesis of base J. We conclude that JBP2 and JBP1 are the TH enzymes involved in J biosynthesis...
- Apicoplast genome of the coccidian Eimeria tenellaXiaomin Cai
Department of Veterinary Pathobiology, College of Veterinary Medicine, College Station, TX 77843 4467, USA
Gene 321:39-46. 2003....
- Reassessment of the epidemiology of amebiasis: state of the artCecilia Ximenez
Departamento de Medicina Experimental, Facultad de Medicina, Universidad Nacional Autonoma de Mexico, Mexico DF, Mexico
Infect Genet Evol 9:1023-32. 2009..Finally, we will discuss those areas of the host-parasite relationship that are still not fully understood, and the scientific challenges to approach this important public health problem in the future...
- Untangling the phylogeny of amoeboid protistsJan Pawlowski
Department of Zoology and Animal Biology, University of Geneva, Geneva, Switzerland
J Eukaryot Microbiol 56:16-25. 2009..Finally, we highlight the interest of Amoebozoa and Rhizaria for understanding eukaryotic evolution and suggest that resolving their phylogenies will be among the main challenges for future phylogenomic analyses...
- Real-time PCR as a new tool for quantifying Leishmania infantum in liver in infected miceS Bretagne
Laboratoire de Parasitologie Mycologie, Hôpital Henri Mondor APHP and Université Paris XII, Creteil, Paris, France
Clin Diagn Lab Immunol 8:828-31. 2001..The correlation coefficient with the microtitration method was 0.66. This PCR assay can be considered for experimental pharmaceutical studies...
- Identification and distribution of Acanthamoeba species genotypes associated with nonkeratitis infectionsGregory C Booton
Department of Evolution, Ecology, Organismal Biology, The Ohio State University, 318 W 12th Ave, Columbus, OH 43210, USA
J Clin Microbiol 43:1689-93. 2005..However, the genotypes that were isolated from brains have not been observed in environmental isolates of Acanthamoeba, and their natural ecological niche is unknown...
- Species-specific loop-mediated isothermal amplification (LAMP) for diagnosis of trypanosomosisOriel M M Thekisoe
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
Acta Trop 102:182-9. 2007..Therefore, LAMP has great potential of being used for diagnosis of trypanosomosis in the laboratory and the field, especially in countries that lack sufficient resources needed for application of molecular diagnostic techniques...
- Leishmania donovani is the only cause of visceral leishmaniasis in East Africa; previous descriptions of L. infantum and "L. archibaldi" from this region are a consequence of convergent evolution in the isoenzyme dataM B Jamjoom
Liverpool School of Tropical Medicine, Pembroke Place, Liverpool, L3 5QA, UK
Parasitology 129:399-409. 2004..donovani endemic areas and identified as L. infantum. It was also concluded that the name L. archibaldi is invalid and that only a single visceralizing species, Leishmania donovani, is found in East Africa...
- Chloroquine-resistant Plasmodium vivax malaria in PeruTrenton K Ruebush
Office of the Director, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30341, USA
Am J Trop Med Hyg 69:548-52. 2003..None of the subjects studied on the northern Pacific Coast had recurrent parasitemia...
- Similar trends of pyrimethamine resistance-associated mutations in Plasmodium vivax and P. falciparumMohammad Tauqeer Alam
Department of Biotechnology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, India
Antimicrob Agents Chemother 51:857-63. 2007..vivax, suggesting that the drug should be used with caution to minimize the development of cross-species resistance in the field...
- American trypanosomiasis (Chagas' disease) and the role of molecular epidemiology in guiding control strategiesMichael A Miles
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT
BMJ 326:1444-8. 2003
- PCR detection of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii in stool samples from Sydney, AustraliaR Fotedar
Department of Microbiology, St Vincent s Hospital, Sydney, Australia
J Clin Microbiol 45:1035-7. 2007..moshkovskii were observed in 63 (70.8%) and 55 (61.8%) patients, respectively, by PCR. This is the first study in Australia using molecular techniques to determine the presence of E. histolytica, E. dispar, and E. moshkovskii...
- Identification of a sporozoite-specific member of the Toxoplasma SAG superfamily via genetic complementationJay R Radke
Department of Veterinary Molecular Biology, Montana State University, Bozeman, Montana 59717 3610, USA
Mol Microbiol 52:93-105. 2004..Expression of SporoSAG in tachyzoites conferred enhanced invasion on transgenic parasites suggesting a role for this protein in oocyst/sporozoite transmission to susceptible hosts...
- Cryptosporidium population genetics: evidence of clonality in isolates from France and HaitiTramy Ngouanesavanh
Ecologie du Parasitisme EA 3609, IFR 142, Institut Pasteur de Lille, 1 rue du Professeur Calmette BP245, 59019 Lille Cedex, France
J Eukaryot Microbiol 53:S33-6. 2006
- Genetic rescue of a Toxoplasma gondii conditional cell cycle mutantMichael W White
Department of Veterinary Molecular Biology, Montana State University, Bozeman, MT 59717 3610, USA
Mol Microbiol 55:1060-71. 2005..Altogether, these results demonstrate that ts11C9 is a conditional mitotic mutant containing a single defect which influences two distinct control points in the T. gondii tachyzoite cell cycle...
- The growing contributions of molecular biology and immunology to protistan ecology: molecular signatures as ecological toolsDavid A Caron
Department of Biological Sciences, University of Southern California, Los Angeles, California 90089 0371, USA
J Eukaryot Microbiol 51:38-48. 2004..These issues include the highly technical nature of some of the molecular methods, the reconciliation of morphology-based and sequence-based species identifications, and the species concept itself...
- Molecular systematics of the parasitic protozoan Giardia intestinalisP T Monis
Department of Microbiology and Immunology, University of Adelaide, Australia
Mol Biol Evol 16:1135-44. 1999..intestinalis, instead placing G. microti within G. intestinalis. The findings support the hypothesis that G. intestinalis is a species complex and suggest that G. microti is a member of this complex...
- Identification of trypanosomes in wild animals from southern Cameroon using the polymerase chain reaction (PCR)S Herder
LRCT IRD CIRAD, TA 207 G, Campus International de Baillarguet, F 34398 Montpellier, France
Parasite 9:345-9. 2002..monticola), in carnivores (Nandinia binotata and Genetta servalina) and in rodents (Cricetomys gambianus and Atherurus africanus). 13 species (54%) were carrying T. brucei s.l. identified as non-gambiense group 1...
- Increased sensitivity of malaria detection by nested polymerase chain reaction using simple sampling and DNA extractionJ Cox-Singh
School of Medical Sciences, University of Science Malaysia, Kelantan, Malaysia
Int J Parasitol 27:1575-7. 1997....
- RNAi-dependent H3K27 methylation is required for heterochromatin formation and DNA elimination in TetrahymenaYifan Liu
Laboratory of Chromatin Biology, The Rockefeller University, New York, New York 10021, USA
Genes Dev 21:1530-45. 2007..These studies provide a framework for an RNAi-dependent, Polycomb group protein-mediated heterochromatin formation pathway in Tetrahymena and underscore the connection between the two highly conserved machineries in eukaryotes...
- The plastid DNA of the malaria parasite Plasmodium falciparum is replicated by two mechanismsD H Williamson
National Institute for Medical Research, Mill Hill, London NW7 1AA, UK
Mol Microbiol 45:533-42. 2002....
- Real-time PCR for detection and identification of Plasmodium sppKathy A Mangold
Department of Pathology and Laboratory Medicine, Evanston Northwestern Healthcare, Evanston, IL 60201, USA
J Clin Microbiol 43:2435-40. 2005..Real-time PCR with melting curve analysis could be a rapid and objective supplement to the examination of Giemsa-stained blood smears and may replace microscopy following further validation...
- Unusual abundance of atypical strains associated with human ocular toxoplasmosisM E Grigg
Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA, USA
J Infect Dis 184:633-9. 2001....
- Congenital Trypanosoma cruzi transmission in Santa Cruz, BoliviaCaryn Bern
Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
Clin Infect Dis 49:1667-74. 2009..We conducted a study of congenital Trypanosoma cruzi infection in Santa Cruz, Bolivia. Our objective was to apply new tools to identify weak points in current screening algorithms, and find ways to improve them...
- Sequence of Plasmodium falciparum chromosomes 2, 10, 11 and 14Malcolm J Gardner
The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, Maryland 20850, USA
Nature 419:531-4. 2002..We report here the nucleotide sequences of chromosomes 10, 11 and 14, and a re-analysis of the chromosome 2 sequence. These chromosomes represent about 35% of the 23-megabase P. falciparum genome...
- Assessing the genetic diversity of the aldolase genes of Plasmodium falciparum and Plasmodium vivax and its potential effect on performance of aldolase-detecting rapid diagnostic testsNelson Lee
The Australian Centre for International and Tropical Health, Brisbane, Australia
J Clin Microbiol 44:4547-9. 2006..vivax aldolases by sequencing the coding genes from parasites of various origins. The results show that aldolases are highly conserved, indicating that antigenic diversity is not a cause of variable RDT sensitivity...
- Real-time PCR for chloroquine sensitivity assay and for pfmdr1-pfcrt single nucleotide polymorphisms in Plasmodium falciparumFrederique de Monbrison
Laboratoire de Parasitologie, Mycologie Med et Pathologie Exotique, Hospices Civils de Lyon, Faculte de Medecine, Universite Claude Bernard, E A 3087, Lyon, France
J Microbiol Methods 54:391-401. 2003....
- Integrated mapping, chromosomal sequencing and sequence analysis of Cryptosporidium parvumAlan T Bankier
Medical Research Council MRC Laboratory of Molecular Biology, Cambridge CB 2 2QH, UK
Genome Res 13:1787-99. 2003..parvum, whereas the other is shared with (but has previously gone unnoticed in) all known genomes of the Coccidia and Haemosporida. These motifs appear to be unique in their structure, distribution and sequences...
- Characterization of the Trypanosoma cruzi Rad51 gene and its role in recombination events associated with the parasite resistance to ionizing radiationCarlos Gustavo Regis-da-Silva
Department of Biochemistry and Immunology, ICB, Universidade Federal de Minas Gerais, Av Antonio Carlos, 6627, Caixa Postal 486, Belo Horizonte 30161 970, MG, Brazil
Mol Biochem Parasitol 149:191-200. 2006..Furthermore, transfected parasites over-expressing TcRad51 have a faster kinetics of recovery of the normal pattern of chromosomal bands after irradiation as well as a higher resistance to zeocin treatment than do wild-type cultures...
- Presence of multiple mRNA cycling sequence element-binding proteins in Crithidia fasciculataBidyottam Mittra
Molecular Biology Institute and Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, California 90095, USA
J Biol Chem 278:26564-71. 2003..Three polypeptides associated with purified CSBP II show specific binding to the cycling sequence. These proteins may represent a family of sequence-specific RNA-binding proteins involved in post-transcriptional regulation...
- Phylogeny, reproductive isolation and kin recognition in the social amoeba Dictyostelium purpureumNatasha J Mehdiabadi
Department of Ecology and Evolutionary Biology, Rice University, Houston, Texas 77005, USA
Evolution 63:542-8. 2009..These observations suggest that cryptic species might occur within D. purpureum and, more importantly, reveal how genetic variation impacts social interactions...
- Erythrocyte invasion by Babesia bovis merozoites is inhibited by polyclonal antisera directed against peptides derived from a homologue of Plasmodium falciparum apical membrane antigen 1Fasila R Gaffar
Department of Infectious Diseases and Immunology, Utrecht University, 3508 TD, Utrecht, The Netherlands
Infect Immun 72:2947-55. 2004....
- Chimpanzee malaria parasites related to Plasmodium ovale in AfricaLinda Duval
Laboratoire de Biologie fonctionnelle des protozoaires, USM 504, Museum national d histoire naturelle, Paris, France
PLoS ONE 4:e5520. 2009..ovale strains isolated from humans. This study provides the first evidence of possibility of natural cross-species exchange of P. ovale between humans and chimpanzees of the subspecies Pan t. troglodytes...
- The ethanolamine branch of the Kennedy pathway is essential in the bloodstream form of Trypanosoma bruceiFederica Gibellini
Division of Biological Chemistry and Drug Discovery, School of Life Sciences, University of Dundee, Dundee, Scotland, UK
Mol Microbiol 73:826-43. 2009..TbECT is therefore genetically validated as a potential drug target against the African trypanosome...
- Multiplex real-time PCR detection of P. falciparum, P. vivax and P. malariae in human blood samplesVincent Veron
Laboratoire Hospitalo universitaire de Parasitologie et Mycologie Médicale, Equipe EA3593, UFR de Medecine, Universite des Antilles et de la Guyane, Cayenne, French Guiana
Exp Parasitol 121:346-51. 2009..Thus, this protocol may prove invaluable for use in the diagnosis of human infection, trial treatments and epidemiologic studies in which high-throughput analyses are often required...
- Complete genome sequence of the apicomplexan, Cryptosporidium parvumMitchell S Abrahamsen
Department of Veterinary and Biomedical Science, College of Veterinary Medicine, University of Minnesota, St Paul, MN 55108, USA
Science 304:441-5. 2004..Elucidation of the core metabolism, including enzymes with high similarities to bacterial and plant counterparts, opens new avenues for drug development...
- Multiple origins and regional dispersal of resistant dhps in African Plasmodium falciparum malariaRichard J Pearce
London School of Hygiene and Tropical Medicine, Department of Infectious Tropical Diseases, London, UK
PLoS Med 6:e1000055. 2009..To that end we have characterised the evolutionary origins of antifolate resistance mutations in the dihydropteroate synthase (dhps) gene and mapped their contemporary distribution...
- Genetic diversity of Plasmodium falciparum histidine-rich protein 2 (PfHRP2) and its effect on the performance of PfHRP2-based rapid diagnostic testsJoanne Baker
Department of Drug Resistance and Diagnostics, Australian Army Malaria Institute, Brisbane, Australia
J Infect Dis 192:870-7. 2005..These findings provide an alternative explanation for the variable sensitivity in field tests of malaria RDTs that is not due to the quality of the RDTs...
- A complex organization of the gene encoding cytochrome oxidase subunit 1 in the mitochondrial genome of the dinoflagellate, Crypthecodinium cohnii: homologous recombination generates two different cox1 open reading framesJ E Norman
Program in Evolutionary Biology, Canadian Institute for Advanced Research, Department of Biochemistry and Molecular Biology, Dalhousie University, Room 8F-2, Tupper Medical Building, 5859 University Avenue, Halifax, Nova Scotia B3H 4H7, Canada
J Mol Evol 53:351-63. 2001..Such recombining repeats are a characteristic feature of plant (angiosperm) mtDNA, but they have not previously been described in the mitochondrial genomes of protists...
- Extensive dynamics of Plasmodium falciparum densities, stages and genotyping profilesAnna Farnert
Unit of Infectious Diseases, Department of Medicine, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden
Malar J 7:241. 2008..The aim of this study was to assess the dynamics of asymptomatic P. falciparum infections in a few hours intervals...
- Neospora caninum-associated abortion in cattle: the time of experimentally-induced parasitaemia during gestation determines foetal survivalD J Williams
Veterinary Parasitology, Liverpool School of Tropical Medicine Faculty of Veterinary Science, University of Liverpool, UK
Parasitology 121:347-58. 2000..These results suggest that the reason some cows abort is related to the time during gestation when they become infected or an existing infection recrudesces...
- HYPOTRICH GENOME IN DEVELOPMENT AND EVOLUTIONDavid Prescott; Fiscal Year: 2001..The experiments deal with the general problem of how an organism can manipulate its own DNA. ..
- NOS2 and arginase in visceral leishmaniasisPeter Melby; Fiscal Year: 2009..These studies in the hamster will fill a knowledge gap by defining mechanisms of disease and immunity that are strikingly different from those of the murine model. ..
- Development of a cell-based method for high-throughput drug screening in malaria Liwang Cui; Fiscal Year: 2008..To facilitate antimalarial drug discovery, we propose to develop a highly sensitive, cell-based, one-step luminescent drug screen assay for high throughput screening of large chemical libraries. [unreadable] [unreadable] [unreadable]..
- Role of iNOS and CAT-2 in defense against CP infectionJody Gookin; Fiscal Year: 2006..parvum infection. The PI is presently in the 3rd year of a 5-year K08 from NIDDK. These studies are anticipated to generate key preliminary data for the PI's first R01 application ..
- Tinidazole for the Treatment of Bacterial VaginosisJane Schwebke; Fiscal Year: 2007..In addition, we will measure vaginal and serum concentrations of drug during treatment and correlate with cure rates, short-term recurrence of BV, and severity of infection. ..
- VACCINATION OF DOGS TO REDUCE TRANSMISSION OF LEISHMANIAPeter Melby; Fiscal Year: 2003..Successful vaccination of dogs in an endemic could reduce both enzootic (among the canine reservoir) and zoonotic (from the canine reservoir to humans) transmission. ..
- Translational Control in Plasmodium Sexual DevelopmentLiwang Cui; Fiscal Year: 2005....
- Comparison of US and European Babesia divergens IsolatesPatricia Holman; Fiscal Year: 2004..The molecular aspects may provide information that will be useful in future efforts to devise diagnostic tests. ..
- NO& Epithelial Repair in CryptosporidiosisJody Gookin; Fiscal Year: 2006..abstract_text> ..
- Plasmodium vivax:Proteomic Analysis of Asexual and Sporozoite StagesLiwang Cui; Fiscal Year: 2007..Its recent resurgence in prevalence and emergence of drug- resistant parasite strains have raised a great concern for the future control of malaria. [unreadable] [unreadable] [unreadable]..
- Therapy and Prevention for Bacterial VaginosisJane Schwebke; Fiscal Year: 2006..We will also utilize specimens from these prospective studies to further study the association of Mobiluncus, an organism strongly associated with BV using sensitive PCR technology. ..
- Transcriptional Regulation in Giardia lambliaHEIDI ELMENDORF; Fiscal Year: 2006..These studies will build an understanding of transcription in Giardia lamblia and lay foundations for study of the role of the regulation of gene expression in disease. ..
- Cryptosporidium parvum DNA replication proteinsGuan Zhu; Fiscal Year: 2004..The completion of these aims will not only prove or refute our hypothesis, but also establish a new foundation for the drug discovery targeting the parasite unique DNA mechanism machinery. ..
- Concordance Rates of Mobiluncus Among Sexual PartnersJane Schwebke; Fiscal Year: 2004..Our hypothesis is that Mobiluncus curtisii is important in the pathogenesis of BV and high concordance rates among sexual partners will be found suggesting sexual transmission. ..
- Control of Trichomoniasis -A Paradigm for STD ControlJane Schwebke; Fiscal Year: 2006..vaginalis, to examine strain differences using PFGE, and to examine the prevalence of resistance strains and their potential contribution to treatment failure. ..
- Fatty Acid Biosynthesis in Cryptosporidium parvumGuan Zhu; Fiscal Year: 2010..3) To validate that fatty acid metabolic enzymes may serve as rational drug target in Cryptosporidium by discovering inhibitors selectively against, parasite fatty acid metabolic enzymes. ..
- Microfilaments in Giardia Attachment and VirulenceHEIDI ELMENDORF; Fiscal Year: 2006..This work will advance our understanding of parasite pathogenesis and develop our knowledge of microfilaments as a target for drug design. ..
- MOLECULAR TOOLS TO DEVELOP HAMSTER MODELS OF DISEASEPeter Melby; Fiscal Year: 2003..This work will have particular relevance to research traditionally funded through a number of NIH institutes including the NCI, NIAID, NHLBI, NIA, NIAMS, and NIDR. ..
- Role of Cryptosporidium Mucins in Host-parasite interactionsROBERTA O CONNOR; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- GCN5-mediated transcription in AIDS pathogen ToxoplasmaWILLIAM SULLIVAN JR; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- Structures of the ends of telomeresMARTIN HORVATH; Fiscal Year: 2007..Insights obtained from the proposed research will thus have significance for understanding human telomeres and the role that telomere structure plays in the biology of aging and cancer. [unreadable] [unreadable]..
- Utility of rapid diagnostic tests for malariaHeidi Hopkins; Fiscal Year: 2007..unreadable] [unreadable]..
- Microarray Analysis of Leptospiral GenomesJoseph Vinetz; Fiscal Year: 2007..unreadable] [unreadable]..
- Identification of Tetrahymena DNA Rearrangement GenesDOUGLAS LEE CHALKER; Fiscal Year: 2010..abstract_text> ..
- Drug resistance in Plasmodium falciparumJohanna Daily; Fiscal Year: 2007..abstract_text> ..
- Comparative Evolutionary Genomics and Infectious DiseaseJane Carlton; Fiscal Year: 2010....
- Human Reservoirs of Plasmodium vivax Transmission in the Peruvian AmazonJoseph M Vinetz; Fiscal Year: 2010..vivax, and provide a rational basis for determining strategies to implement and deploy new transmission- blocking vaccines as one approach towards the future elimination of P. vivax malaria from the Amazon basin. ..
- Development of Assay for High Throughput Screen of Parasite Glucose TransporterScott M Landfear; Fiscal Year: 2010..Since glucose is an essential nutrient for these parasites, such compounds would kill the parasite and could act as novel drugs for treatment of the diseases they cause. ..
- Functional Characterization of Leishmania TransportersScott M Landfear; Fiscal Year: 2010..Overall, this proposal will provide a detailed biochemical and genetic dissection of glucose transporter function and the essential role of these permeases in the parasite life cycle. ..
- Recognition coding at CNS synapsesGreg R Phillips; Fiscal Year: 2010..Upon completion of these Aims we expect to have a better understanding of the role of cell-cell interactions in synaptogenesis. ..
- Beyond GFP and aequorin: Ocean-wide study of fluorescent and luminous proteinsMikhail Matz; Fiscal Year: 2009..e., sharing no homology with the previously cloned ones) genetically encoded fluorescent and luminescent markers which may become a basis for the new generation of in vivo labeling and detection technologies. ..
- Developmental regulation of DNA replication during programmed gene ampplificationGeoffrey Kapler; Fiscal Year: 2008..unreadable] [unreadable] [unreadable] [unreadable]..
- APEs as novel drug targets in AIDS opportunistic pathogen ToxoplasmaWILLIAM SULLIVAN JR; Fiscal Year: 2008..Moreover, Toxoplasma can be informative as a model organism to study Plasmodium, the causative agent of malaria. [unreadable] [unreadable] [unreadable] [unreadable]..
- Transcriptional mechanisms in Toxoplasma gondiiMichael White; Fiscal Year: 2008..unreadable] [unreadable] [unreadable]..
- GENETIC DISSECTION OF TOXOPLASMA GONDIIDavid Roos; Fiscal Year: 2008..In addition to their value for the analysis of folate metabolism and drug resistance, these tools should be broadly applicable to other studies on the biology and biochemistry of Toxoplasma. ..
- International Leptospirosis Society Meeting 2007Joseph Vinetz; Fiscal Year: 2007..unreadable] Outcome of the meeting will be assessed by peer-reviewed publication and trainee publication and[unreadable] career advancement. ..
- Antigen Detection Assay for TuberculosisAntonio Campos Neto; Fiscal Year: 2006..unreadable] [unreadable] [unreadable]..
- Calcium ATPases in Apicomplexan Parasites as Potential DL Sibley; Fiscal Year: 2006..Knowledge gained through these studies may improve the ability to pharmacologically inhibit key calcium regulatory enzymes and hence block motility and invasion by this important group of parasites. ..
- Adhesins and Invasins of Francisella TularensisBarbara Mann; Fiscal Year: 2004..Understanding the basic mechanisms of pathogenicity will lead to a greater understanding of why this organism is such an effective pathogen, and the identification of new targets for therapies. ..
- Study of permissive/non-permissive T. gondii infectionsJay Radke; Fiscal Year: 2004..abstract_text> ..
- Pathogenesis of falciparum malaria in infancyPatrick Duffy; Fiscal Year: 2004..Identifying new therapies for children is an urgent public health goal, but is not a funding priority for the United States Department of Defense. ..
- MOLECULAR ANALYSIS OF ANTIGENIC VARIATION IN MALARIAMary Galinski; Fiscal Year: 2004....
- Shotgun Sequencing of the Trichomonas Vaginalis GenomeJane Carlton; Fiscal Year: 2003..A publically accessible, user-friendly web site will be created for access to the genome and EST data during the project, and for access to the final finished genome sequence and annotation at the conclusion of the project. ..
- 5'/3'-SAGE analysis of gene expression in ToxoplasmaMichael White; Fiscal Year: 2003..abstract_text> ..
- Abnormalities of Mitochondria in Chagasic MyocarditisNisha Garg; Fiscal Year: 2003..The proposed studies will determine the pathophysiological contribution of host mitochondrial abnormalities in the progression of chagas disease. ..
- IN VIVO INDUCED ANTIGEN TECHNOLOGY OF AA IN LJPMartin Handfield; Fiscal Year: 2003..In vivo induced antigens discovered using this method are excellent candidates for consideration as potential diagnostic tools, new targets for antimicrobial therapy, or for vaccine design. ..
- GENE DISCOVERY IN TOXOPLASMA GONDIIL Sibley; Fiscal Year: 2003..In combination with the genetic tools available in T. gondii, this will greatly accelerate investigations into the complex developmental biology of apicomplexan parasites. ..
- GALACTOSE LECTIN OF E HISTOLYTICABarbara Mann; Fiscal Year: 2002..Successful completion of these experiments will provide insight into the role and identity of PTK/substrates in lectin-mediated killing and may establish the basis for novel therapeutic or prophylactic measures against amebiasis. ..
- Telomere structure and function in Trypanosoma bruceiGEORGE CROSS; Fiscal Year: 2005..There are significant similarities between the telomeres of trypanosomes and humans, so trypanosomes would be a useful model in which to study events that are relevant to human aging and cancer. ..
- VACCINE DEVELOPMENT IN TUBERCULOSISAntonio Campos Neto; Fiscal Year: 2004..In addition to the evaluation of these antigens, we will characterize and clone other vaccine candidates using recently developed approaches for the direct identification of both CD4 and CD8 T cell antigens. ..
- Confocal Microscope for MicrobiologyL Sibley; Fiscal Year: 2002..This instrument will greatly strengthen the research programs of these highly productive groups by applying high-resolution confocal microscopy to the study of human pathogens...
- GFP-like proteins:Diversity and determinants of colorMikhail Matz; Fiscal Year: 2006..This would greatly facilitate rational design of GFP-like reporters. ..
- A Genome Sequence Survey of Enterocytozoon bieneusiDonna Akiyoshi; Fiscal Year: 2006..c. Establishment of a website to provide access to the sequence data and clones by the scientific community. ..
- "Vivax Malaria Research: 2005 and Beyond"Jane Carlton; Fiscal Year: 2005..vivax with P. falciparum, epidemiology and diversity studies, vectors and transmission dynamics, and a bioinformatics workshop for researchers and students to learn to manipulate Plasmodium sequence data. ..
- DEVELOPMENT OF GENETIC TOOLS FOR TRYPANOSOMA BRUCEIGEORGE CROSS; Fiscal Year: 2006..abstract_text> ..
- Chromosome breakage and DNA detection in TetrahymenaMeng Chao Yao; Fiscal Year: 2006..abstract_text> ..
- MOLECULAR TARGETS OF BLOCKING MALARIA TRANSMISSIONJoseph Vinetz; Fiscal Year: 2005....
- Purine salvage in Cryptosporidium parvumMitchell Abrahamsen; Fiscal Year: 2005..In addition, these studies demonstrate our ability to use heterologous expression of C. parvum genes in yeast as a means to develop a high-throughput screening assay for the identification of specific inhibitors. ..
- Mechanisms of adhesion and recognition at CNS synapsesGreg Phillips; Fiscal Year: 2005..We will use these reagents to examine the role of cell-cell interactions in the initiation, maturation and plasticity of synaptic junctions and in the recruitment of other critical molecules to synaptic membranes. ..
- Gene Duplication and Genome EvolutionAustin Hughes; Fiscal Year: 2004..5) Examining the role of transposable elements in duplication of genomic segments by testing for nonrandom association between these elements and putatively duplicated blocks in the yeast genome. ..
- Cryptosporidium parvum Histone DeacetylasesSTANLEY RIDER; Fiscal Year: 2007..There are currently no highly effective treatments for Cryptosporidium parvum. This research is a beginning step toward identifying novel methods to control this parasite. [unreadable] [unreadable] [unreadable]..
- EVOLUTION OF IMMUNE RECOGNITION AND EVASIONAustin L Hughes; Fiscal Year: 2010..In addition, by increasing our understanding of the population diversity and evolutionary history of human immune system genes, this research will enhance our knowledge of human immune function in health and disease. ..
- Antigenic Variation and Drug Resistance In P. falciparumQin Cheng; Fiscal Year: 2006..The project has the potential to benefit the health of a substantial proportion of the world's population. ..
- PLASMODIUM VIVAX MSP-3 AND MSP-9 AS VACCINE IMMUNOGENESMary Galinski; Fiscal Year: 2007..vivax vaccine candidates based on these antigens and also serve to establish reliable procedures to test and evaluate antigens in non-human primate models for safety, immunogenicity and efficacy. ..
- Trypanosomatid Mitochondrial DNA PolymerasesMichele Klingbeil; Fiscal Year: 2008..The T. brucei pol I-like protein family also offers the unique opportunity to uncover insights into the evolutionary strategies used to produce a replicative polymerase. ..