ribosomal spacer dna

Summary

Summary: The intergenic DNA segments that are between the ribosomal RNA genes (internal transcribed spacers) and between the tandemly repeated units of rDNA (external transcribed spacers and nontranscribed spacers).

Top Publications

  1. ncbi Uniting Tricholoma sulphureum and T. bufonium
    Ornella Comandini
    Dipartimento di Scienze Ambientali, Universita dell Aquila, Via Vetoio, Loc Coppito, I 67100 L Aquila, Italy
    Mycol Res 108:1162-71. 2004
  2. ncbi Ribosomal ITS sequences and plant phylogenetic inference
    I Alvarez
    Department of Botany, Iowa State University, Ames, IA 50011, USA
    Mol Phylogenet Evol 29:417-34. 2003
  3. ncbi A two-locus global DNA barcode for land plants: the coding rbcL gene complements the non-coding trnH-psbA spacer region
    W John Kress
    Department of Botany, National Museum of Natural History, Smithsonian Institution, Washington, DC, United States of America
    PLoS ONE 2:e508. 2007
  4. ncbi Reviewing lymnaeid vectors of fascioliasis by ribosomal DNA sequence analyses
    M D Bargues
    Departamento de Parasitologia, Facultad de Farmacia, Universidad de Valencia, Av Vicente Andrés Estellés s n, 46100 Burjassot, Valencia, Spain
    J Helminthol 79:257-67. 2005
  5. ncbi Inferring the history of the polyploid Silene aegaea (Caryophyllaceae) using plastid and homoeologous nuclear DNA sequences
    M Popp
    Department of Systematic Botany, Uppsala University, Uppsala, SE-752 36, Sweden
    Mol Phylogenet Evol 20:474-81. 2001
  6. ncbi Only a few fungal species dominate highly diverse mycofloras associated with the common reed
    Karin Neubert
    Lehrstuhl Phytopathologie, Fachbereich Biologie, , , D-78457 Konstanz, Germany
    Appl Environ Microbiol 72:1118-28. 2006
  7. ncbi Hyperdiversity of ectomycorrhizal fungus assemblages on oak seedlings in mixed forests in the southern Appalachian Mountains
    John F Walker
    Department of Biology, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24060, USA
    Mol Ecol 14:829-38. 2005
  8. ncbi Development of a DNA microarray for detection and identification of fungal pathogens involved in invasive mycoses
    Dirk M Leinberger
    Institute of Technical Biochemistry, University of Stuttgart, Allmandring 31, Stuttgart 70569, Germany
    J Clin Microbiol 43:4943-53. 2005
  9. ncbi European Lymnaeidae (Mollusca: Gastropoda), intermediate hosts of trematodiases, based on nuclear ribosomal DNA ITS-2 sequences
    M D Bargues
    , Facultad de Farmacia, Universidad de Valencia, , 46100 Burjassot, Valencia, Spain
    Infect Genet Evol 1:85-107. 2001
  10. ncbi Characteristics of the nuclear (18S, 5.8S, 28S and 5S) and mitochondrial (12S and 16S) rRNA genes of Apis mellifera (Insecta: Hymenoptera): structure, organization, and retrotransposable elements
    J J Gillespie
    Department of Entomology, Texas A and M University, College Station, TX, USA
    Insect Mol Biol 15:657-86. 2006

Research Grants

  1. Evaluating Trichophyton tonsurans Carriage and Infection
    Susan Abdel Rahman; Fiscal Year: 2006
  2. FUNGAL PROTEIN/GLYCOPROTEIN EXPRESSION IN TINEA CAPITIS
    Susan Abdel Rahman; Fiscal Year: 2003
  3. High through-put detection of fungal pathogens
    JACK FELL; Fiscal Year: 2004
  4. Genetics of Cryptosporidium parvum
    Giovanni Widmer; Fiscal Year: 2006
  5. A Genome Sequence Survey of Enterocytozoon bieneusi
    Donna Akiyoshi; Fiscal Year: 2006
  6. LARVAL MOSQUITO FEEDING AND MICROBIAL INTERACTIONS
    Edward Walker; Fiscal Year: 2006
  7. Role of non-tuberculous mycobacteria in lung disease
    Jason Stout; Fiscal Year: 2006
  8. Microbial Mediation of Habitat Selection and Production
    Edward Walker; Fiscal Year: 2006
  9. Subjective Symptoms after Treatment of Lyme Disease
    Gary P Wormser; Fiscal Year: 2010
  10. Insecticide mosaics and sustainability of treated nets
    Edward Walker; Fiscal Year: 2007

Detail Information

Publications212 found, 100 shown here

  1. ncbi Uniting Tricholoma sulphureum and T. bufonium
    Ornella Comandini
    Dipartimento di Scienze Ambientali, Universita dell Aquila, Via Vetoio, Loc Coppito, I 67100 L Aquila, Italy
    Mycol Res 108:1162-71. 2004
    ..We conclude that T. bufonium cannot be maintained as an autonomous taxon and should be treated as an infraspecific variant of T. sulphureum...
  2. ncbi Ribosomal ITS sequences and plant phylogenetic inference
    I Alvarez
    Department of Botany, Iowa State University, Ames, IA 50011, USA
    Mol Phylogenet Evol 29:417-34. 2003
    ..It is suggested that more robust insights are likely to emerge from the use of single-copy or low-copy nuclear genes...
  3. ncbi A two-locus global DNA barcode for land plants: the coding rbcL gene complements the non-coding trnH-psbA spacer region
    W John Kress
    Department of Botany, National Museum of Natural History, Smithsonian Institution, Washington, DC, United States of America
    PLoS ONE 2:e508. 2007
    ..This low rate has complicated the trade-off in finding a locus that is universal and readily sequenced and has sufficiently high sequence divergence at the species-level...
  4. ncbi Reviewing lymnaeid vectors of fascioliasis by ribosomal DNA sequence analyses
    M D Bargues
    Departamento de Parasitologia, Facultad de Farmacia, Universidad de Valencia, Av Vicente Andrés Estellés s n, 46100 Burjassot, Valencia, Spain
    J Helminthol 79:257-67. 2005
    ....
  5. ncbi Inferring the history of the polyploid Silene aegaea (Caryophyllaceae) using plastid and homoeologous nuclear DNA sequences
    M Popp
    Department of Systematic Botany, Uppsala University, Uppsala, SE-752 36, Sweden
    Mol Phylogenet Evol 20:474-81. 2001
    ..pentelica as the maternal lineage. The strength of the approach of using homoeologous sequences of several loci is demonstrated, and its usefulness for the study of phylogenies of groups including polyploids is emphasized...
  6. ncbi Only a few fungal species dominate highly diverse mycofloras associated with the common reed
    Karin Neubert
    Lehrstuhl Phytopathologie, Fachbereich Biologie, , , D-78457 Konstanz, Germany
    Appl Environ Microbiol 72:1118-28. 2006
    ..This suggestion was independently supported by the results of nested PCR assays specifically monitoring two OTUs over 3 years, which revealed significant preferences for host habitat and host organ...
  7. ncbi Hyperdiversity of ectomycorrhizal fungus assemblages on oak seedlings in mixed forests in the southern Appalachian Mountains
    John F Walker
    Department of Biology, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24060, USA
    Mol Ecol 14:829-38. 2005
    ..The high mycobiont diversity highlights the potential for seedlings to acquire carbon from mycelial networks and confirms the utility of using outplanted seedlings to estimate ectomycorrhizal diversity...
  8. ncbi Development of a DNA microarray for detection and identification of fungal pathogens involved in invasive mycoses
    Dirk M Leinberger
    Institute of Technical Biochemistry, University of Stuttgart, Allmandring 31, Stuttgart 70569, Germany
    J Clin Microbiol 43:4943-53. 2005
    ..As the microarray was able to detect and clearly identify the fungal pathogens within 4 h after DNA extraction, this system offers an interesting potential for clinical microbiology laboratories...
  9. ncbi European Lymnaeidae (Mollusca: Gastropoda), intermediate hosts of trematodiases, based on nuclear ribosomal DNA ITS-2 sequences
    M D Bargues
    , Facultad de Farmacia, Universidad de Valencia, , 46100 Burjassot, Valencia, Spain
    Infect Genet Evol 1:85-107. 2001
    ..Similarly, ITS-2 sequencing would allow a step forward in the appropriate rearrangement of the actual systematic confusion among echinostomatids...
  10. ncbi Characteristics of the nuclear (18S, 5.8S, 28S and 5S) and mitochondrial (12S and 16S) rRNA genes of Apis mellifera (Insecta: Hymenoptera): structure, organization, and retrotransposable elements
    J J Gillespie
    Department of Entomology, Texas A and M University, College Station, TX, USA
    Insect Mol Biol 15:657-86. 2006
    ....
  11. ncbi Pan-eukaryote ITS2 homologies revealed by RNA secondary structure
    Annette W Coleman
    Division of Biology and Medicine, Brown University, Providence, RI 02912, USA
    Nucleic Acids Res 35:3322-9. 2007
    ..Equally important, the recognition of eukaryote-wide homology regions provides extensive and detailed information to test experimental studies of ribosomal rRNA processing...
  12. ncbi ITS2 data corroborate a monophyletic chlorophycean DO-group (Sphaeropleales)
    Alexander Keller
    Department of Bioinformatics, University of Wurzburg, Am Hubland, 97074 Wurzburg, Germany
    BMC Evol Biol 8:218. 2008
    ....
  13. ncbi A Beauveria phylogeny inferred from nuclear ITS and EF1-alpha sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs
    Stephen A Rehner
    Insect Biocontrol Laboratory, USDA ARS, Beltsville, Maryland 20705, USA
    Mycologia 97:84-98. 2005
    ..These results establish a phylogenetic framework for further taxonomic, phylogenetic and comparative biological investigations of Beauveria and their corresponding Cordyceps teleomorphs...
  14. ncbi ITS2 sequence-structure analysis in phylogenetics: a how-to manual for molecular systematics
    Jörg Schultz
    Department of Bioinformatics, Biocenter, University of Wurzburg, Am Hubland, D 97074 Wurzburg, Germany
    Mol Phylogenet Evol 52:520-3. 2009
    ..Moreover, compensatory base changes (CBCs) in ITS2 sequence-structure pairs are identified as a possible marker for distinguishing species...
  15. ncbi Reproductive isolation among sympatric cryptic species in marine diatoms
    Alberto Amato
    Stazione Zoologica A Dohrn, Villa Comunale, 80121 Napoli, Italy
    Protist 158:193-207. 2007
    ..Our findings not only show that cryptic genetic variants abound in sympatry, but also that they are reproductively isolated and, therefore, biologically distinct units...
  16. ncbi Diversity and phylogenetic affinities of foliar fungal endophytes in loblolly pine inferred by culturing and environmental PCR
    A Elizabeth Arnold
    Department of Biology, Duke University, Durham, North Carolina 27708, USA
    Mycologia 99:185-206. 2007
    ....
  17. ncbi The ITS2 Database II: homology modelling RNA structure for molecular systematics
    Christian Selig
    Department of Bioinformatics, Biocenter, University of Wurzburg, Am Hubland 97074 Würzburg, Germany
    Nucleic Acids Res 36:D377-80. 2008
    ..These genera might have evolved an ITS2 structure diverging from the standard one. The current version of the ITS2 Database can be accessed via http://its2.bioapps.biozentrum.uni-wuerzburg.de...
  18. ncbi Non-homogenized ITS regions in the parasitic nematode Cooperia oncophora
    T H M Mes
    Department of Parasitology and Tropical Veterinary Medicine, Institute of Infectious Diseases and Immunology, Utrecht University, 3508 TD Utrecht, The Netherlands
    Parasitology 129:213-22. 2004
    ..Finally, although it can not be rigorously assessed in Cooperia, the ITS polymorphisms can readily be envisioned to affect phylogenetic reconstructions of closely related nematodes...
  19. ncbi Unusual compact rDNA gene arrangements within some members of the Ascomycota: evidence for molecular co-evolution between ITS1 and ITS2
    Georg Hausner
    Department of Microbiology, University of Mannitoba, Winnipeg, Canada
    Genome 48:648-60. 2005
    ..This supports biochemical evidence indicating that the ITS1 and ITS2 segments interact to facilitate the maturation of the rRNA precursor...
  20. ncbi Molecular characterization of nontuberculous mycobacteria isolated from human cases of disseminated disease in the USA, Thailand, Malawi, and Tanzania
    Jason Ballard
    National Reference Centre for Mycobacteriology, National Microbiology Laboratory, Health Canada, Winnipeg, Manitoba, Canada
    J Gen Appl Microbiol 53:153-7. 2007
  21. ncbi Lower level relationships in the mushroom genus Cortinarius (Basidiomycota, Agaricales): a comparison of RPB1, RPB2, and ITS phylogenies
    T G Frøslev
    Department of Microbiology, Institute of Biology, University of Copenhagen, Øster Farimagsgade 2D, DK 1353 Copenhagen K, Denmark
    Mol Phylogenet Evol 37:602-18. 2005
    ..We speculate that sequence information from RNA polymerase II genes have the potential for resolving phylogenetic problems at several levels of the diverse and taxonomically very challenging genus Cortinarius...
  22. ncbi Termitomyces sp. associated with the termite Macrotermes natalensis has a heterothallic mating system and multinucleate cells
    Henrik H De Fine Licht
    Department of Population Biology, Biological Institute, University of Copenhagen, DK-2100 Copenhagen O, Denmark
    Mycol Res 109:314-8. 2005
    ..This is the first time the mating system of a Termitomnyces species has been studied...
  23. ncbi Polygonum viviparum mycobionts on an alpine primary successional glacier forefront
    Oliver Mühlmann
    Institute of Microbiology, University Innsbruck, Technikerstr 25, Innsbruck, Austria
    Mycorrhiza 18:87-95. 2008
    ..We regard the presence/absence of fungal partner and limiting abiotic impacts of the environment as key factors for the symbiotic status of P. viviparum...
  24. ncbi ITS2 sequences as barcodes for identifying and analyzing spider mites (Acari: Tetranychidae)
    Tselila Ben David
    Department of Entomology, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot 76100, Israel
    Exp Appl Acarol 41:169-81. 2007
    ..We predict that our work, together with similar efforts, will provide in the future the platform for a uniform, accurate, practical and easy-to-use method of spider mite species identification...
  25. ncbi Native range genetic variation in Arabidopsis thaliana is strongly geographically structured and reflects Pleistocene glacial dynamics
    James B Beck
    Department of Biology, Washington University, St Louis, MO 63130, USA
    Mol Ecol 17:902-15. 2008
    ..Spatial autocorrelation analyses indicate that isolation by distance is pervasive at all hierarchical levels, but that it is reduced in portions of Europe...
  26. ncbi Paramecium aurelia revisited
    A W Coleman
    Division of Biology and Medicine, Brown University, Providence, Rhode Island 02912, USA
    J Eukaryot Microbiol 52:68-77. 2005
    ....
  27. ncbi Fungal communities in mycorrhizal roots of conifer seedlings in forest nurseries under different cultivation systems, assessed by morphotyping, direct sequencing and mycelial isolation
    Audrius Menkis
    Department Forest Mycology and Pathology, Swedish University of Agricultural Sciences, Uppsala, Sweden
    Mycorrhiza 16:33-41. 2005
    ..There was a moderate similarity in mycorrhizal communities between pine and spruce and among different cultivation systems...
  28. ncbi Molecular phylogeny of the Pseudallescheria boydii species complex: proposal of two new species
    Felix Gilgado
    Unitat de Microbiologia Facultat de Medicina i Ciencias de la Salut, , 43201 Reus, Tarragona, Spain
    J Clin Microbiol 43:4930-42. 2005
    ..minutispora are environmental. Further studies are needed to demonstrate whether all the species included in the P. boydii complex have different clinical spectra and antifungal susceptibility...
  29. ncbi Biogeographical and phylogenetic origins of African fig species (Ficus section Galoglychia)
    Nina Rønsted
    Jodrell Laboratory, Royal Botanic Gardens, Kew, Richmond, Surrey TW9 3DS, UK
    Mol Phylogenet Evol 43:190-201. 2007
    ..The other main clade includes members of subsections Caulocarpae, Cyathistipulae, Crassicostae and Galoglychia, which are concentrated in West and Central Africa...
  30. ncbi Characterization of Cryptocaryon irritans isolates from marine fishes in Mainland China by ITS ribosomal DNA sequences
    H Y Sun
    School of Life Sciences, State Key Laboratory of Biocontrol Sun Yat-sen University, 135 Xingang West Street, Haizhu District, Guangzhou 510275, Guangdong Province, People's Republic of China
    Parasitol Res 99:160-6. 2006
    ..irritans from Mainland China and elsewhere and is also relevant to the diagnosis and control of fish diseases they cause...
  31. ncbi Rapid identification of Trichophyton tonsurans by specific PCR based on DNA sequences of nuclear ribosomal internal transcribed spacer (ITS) 1 region
    Eri Yoshida
    Department of Dermatology, Tokyo Medical University, Nishishinjuku, 6-1-1 Shinjuku-ku, and Department of Dermatology, Fraternity Memorial Hospital, Tokyo 160-0023, Japan
    J Dermatol Sci 42:225-30. 2006
    ..This T. tonsurans-specific PCR method was able to detect 10 pg of T. tonsurans genomic DNA with ethidium bromide staining. CONCLUSIONS: A PCR identification system specific for T. tonsurans is rapid, sensitive, and specific...
  32. ncbi A survey of nuclear ribosomal internal transcribed spacer substitution rates across angiosperms: an approximate molecular clock with life history effects
    Kathleen M Kay
    Department of Plant Biology, Michigan State University, 166 Plant Biology Building, East Lansing, MI 48824, USA
    BMC Evol Biol 6:36. 2006
    ..Here, we systematically review the literature to assess substitution rate variation in nrITS among angiosperms, and we evaluate possible correlates of the variation...
  33. ncbi Recent oceanic long-distance dispersal and divergence in the amphi-Atlantic rain forest genus Renealmia L.f. (Zingiberaceae)
    Tiina E Särkinen
    Royal Botanic Garden Edinburgh, 20a Inverleith Row, EH3 5LR Edinburgh, UK
    Mol Phylogenet Evol 44:968-80. 2007
    ..Speciation has been approximately simultaneous on both sides of the Atlantic, but increased taxon sampling is required to compare the speciation rates between the New World and Old World tropics...
  34. ncbi Sequence comparison of distal and proximal ribosomal DNA arrays in rice (Oryza sativa L.) chromosome 9S and analysis of their flanking regions
    Masaki Fujisawa
    Rice Genome Research Program, National Institute of Agrobiological Sciences/Institute of the Society for Techno-innovation of Agriculture, Forestry and Fisheries, Tsukuba, Ibaraki, 305-8602, Japan
    Theor Appl Genet 113:419-28. 2006
    ..This study reveals the repetitive nature of the telomeric end of the short arm of chromosome 9, which consists of telomeric repeats, an rDNA array, and a retrotransposon-rich chromosomal region...
  35. ncbi Mycobacterium sherrisii isolation from a patient with pulmonary disease
    Enrico Tortoli
    Regional Reference Center for Mycobacteria, Careggi Hospital, 50134 Florence, Italy
    Diagn Microbiol Infect Dis 57:221-3. 2007
    ..One of our strains was responsible for pulmonary disease in a middle-aged non-HIV patient; thus, confirming the potential pathogenicity of this species previously reported only in an HIV-positive patient...
  36. ncbi Molecular identification of zygomycetes from culture and experimentally infected tissues
    Patrick Schwarz
    , , CNRS FRE2849, Institut Pasteur, 25 rue du Dr. Roux, 75724 Paris Cedex 15, France
    J Clin Microbiol 44:340-9. 2006
    ....
  37. ncbi Presumptive horizontal symbiont transmission in the fungus-growing termite Macrotermes natalensis
    H H de Fine Licht
    Department of Population Biology, Institute of Biology, University of Copenhagen, Universitetsparken 15, 2100 Copenhagen, Denmark
    Mol Ecol 15:3131-8. 2006
    ..natalensis mounds puzzling. To our knowledge, this is the first detailed study of the genetic population structure of a single lineage of Termitomyces...
  38. ncbi Genetically closely related but phenotypically divergent Trichoderma species cause green mold disease in oyster mushroom farms worldwide
    Monika Komon Zelazowska
    Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Getreidemarkt 9 1665, A 1060 Vienna, Austria
    Appl Environ Microbiol 73:7415-26. 2007
    ..A DNA BarCode for identification of these species based on ITS1 and ITS2 sequences has been provided and integrated in the main database for Hypocrea/Trichoderma (www.ISTH.info)...
  39. ncbi Homology modeling revealed more than 20,000 rRNA internal transcribed spacer 2 (ITS2) secondary structures
    Matthias Wolf
    Department of Bioinformatics, University of Wurzburg, Biocenter, Am Hubland, Germany
    RNA 11:1616-23. 2005
    ..bioapps.biozentrum.uni-wuerzburg.de...
  40. ncbi Unusual structure of ribosomal DNA in the copepod Tigriopus californicus: intergenic spacer sequences lack internal subrepeats
    R S Burton
    Marine Biology Research Division 0202, Scripps Institution of Oceanography, University of California, San Diego, La Jolla, CA 92093 0202, USA
    Gene 344:105-13. 2005
    ....
  41. ncbi Postgenomic taxonomy of human ureaplasmas -- a case study based on multiple gene sequences
    Fanrong Kong
    Centre for Infectious Diseases and Microbiology CIDM, Institute of Clinical Pathology and Medical Research ICPMR, Westmead, New South Wales, Australia
    Int J Syst Evol Microbiol 54:1815-21. 2004
    ..It is recommended that, to avoid confusion, the new nomenclature be used for human ureaplasmas in future publications...
  42. ncbi Mycobionts of Salix herbacea on a glacier forefront in the Austrian Alps
    Oliver Mühlmann
    Institute of Microbiology, University Innsbruck, Technikerstr 25, 6020 Innsbruck, Austria
    Mycorrhiza 18:171-80. 2008
    ..This suggests that the mycorrhizae were displaced from the roots by an outcompeting species, whereas the mycelium was still present in the soil...
  43. ncbi Development and evaluation of a species diagnostic polymerase chain reaction-restriction fragment-length polymorphism procedure for cryptic members of the Culex sitiens (Diptera: Culicidae) subgroup in Australia and the southwest Pacific
    N W Beebe
    Molecular Parasitology Unit, University of Technology, Sydney, New South Wales, Australia
    J Med Entomol 39:362-9. 2002
    ..Overlapping adult morphology was observed for Cx. annulirostris and Cx. palpalis in both northern Queensland and PNG and for all three species at one site in northwest Queensland...
  44. ncbi Three nonorthologous ITS1 types are present in a polypore fungus Trichaptum abietinum
    Kwan Soo Ko
    School of Biological Sciences, Seoul National University, San 56 1 Shinrim Dong, Kwanag gu, Seoul 151 742, Republic of Korea
    Mol Phylogenet Evol 23:112-22. 2002
    ..However, Type III was amplified only in three T. abietinum strains collected from Korea, indicating that a new local geographic subtype has arisen in Korean strains...
  45. ncbi Intraspecific and within-isolate sequence variation in the ITS rRNA gene region of Pythium mercuriale sp. nov. (Pythiaceae)
    Lassaad Belbahri
    Plants and Pathogens Group, Research Institute Earth Nature and Landscape, School of Engineering of Lullier, University of Applied Sciences of Western Switzerland, Jussy, Switzerland
    FEMS Microbiol Lett 284:17-27. 2008
    ..Maximum likelihood phylogenetic analyses based on both ITS and beta-tubulin sequence data place P. mercuriale in a clade between Pythium and Phytophthora...
  46. ncbi Marked intragenomic heterogeneity and geographical differentiation of nrDNA ITS in Larix potaninii (Pinaceae)
    Xiao-Xin Wei
    Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, 20 Nanxincun, Xiangshan, Beijing 100093, China
    J Mol Evol 57:623-35. 2003
    ..In addition, some varieties of L. potaninii might have experienced an nrDNA founder effect parallel to their geographical isolation...
  47. ncbi SSCP-based identification of members within the Pseudoterranova decipiens complex (Nematoda: Ascaridoidea: Anisakidae) using genetic markers in the internal transcribed spacers of ribosomal DNA
    X Q Zhu
    Department of Veterinary Science, The University of Melbourne, Werribee, Victoria, Australia
    Parasitology 124:615-23. 2002
    ....
  48. ncbi Polymerase chain reaction-based identification and genotyping of Anopheles mosquitoes with a 96-pin bacterial replicator
    Cristina S Rafferty
    Centers for Disease Control and Prevention, Division of Parasitic Diseases, Atlanta, Georgia, USA
    Am J Trop Med Hyg 66:234-7. 2002
    ..This method allows rapid analysis of large numbers of mosquitoes without robotic equipment and should enable rapid and extensive PCR analysis of field-collected samples and laboratory specimens...
  49. ncbi ITS2 rRNA evolution and its congruence with the phylogeny of muricid neogastropods (Caenogastropoda, Muricoidea)
    Marco Oliverio
    Dipartimento di Biologia Animale e dell'Uomo, La Sapienza University, , I-00185 Roma, Italy
    Mol Phylogenet Evol 25:63-9. 2002
  50. ncbi Intragenomic variation in the internal transcribed spacer 1 region of Dientamoeba fragilis as a molecular epidemiological marker
    Aldert Bart
    Section Parasitology, Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, Meibergdreef 9, Amsterdam 1105 AZ, The Netherlands
    J Clin Microbiol 46:3270-5. 2008
    ..fragilis directly from feces. This may serve as a means of studying the transmission, geographical distribution, and relationships between strains and the pathogenicity of this parasite...
  51. ncbi Slow algae, fast fungi: exceptionally high nucleotide substitution rate differences between lichenized fungi Omphalina and their symbiotic green algae Coccomyxa
    Stefan Zoller
    Department of Biology, Duke University, Durham, NC 27708 0338, USA
    Mol Phylogenet Evol 29:629-40. 2003
    ....
  52. ncbi Organelle DNA phylogeography of Cycas taitungensis, a relict species in Taiwan
    S Huang
    Department of Biology, National Taiwan Normal University, Taipei
    Mol Ecol 10:2669-81. 2001
    ....
  53. ncbi Ribosomal DNA spacer genotypes of the Anopheles bancroftii group (Diptera: Culicidae) from Australia and Papua New Guinea
    N W Beebe
    Department of Cell and Molecular Biology, University of Technology Sydney, Australia
    Insect Mol Biol 10:407-13. 2001
    ..Genotypes B and C and genotypes C and D were found sympatric and appeared to be evolving independently suggesting the possibility of cryptic species. Genotype C contained two ITS2 sequence types within the genome...
  54. ncbi Molecular phylogeny of hybridizing species from the genus Spartina Schreb. (Poaceae)
    A Baumel
    UMR, CNRS, , , Rennes, France
    Mol Phylogenet Evol 22:303-14. 2002
    ..alterniflora and S. foliosa) on one hand, and alloploid speciation involving more differentiated species (S. alterniflora and S. maritima) on the other hand...
  55. ncbi Organization, differential expression and methylation of rDNA in artificial Solanum allopolyploids
    Nataliya Y Komarova
    Department of General Genetics, Center of Plant Molecular Biology (ZMBP, , Auf der Morgenstelle 28, , 72076, Germany
    Plant Mol Biol 56:439-63. 2004
    ....
  56. ncbi An oligonucleotide barcode for species identification in Trichoderma and Hypocrea
    Irina S Druzhinina
    Division of Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Getreidemarkt 9 1665, A 1060 Vienna, Austria
    Fungal Genet Biol 42:813-28. 2005
    ..We conclude that oligonucleotide barcode is a powerful tool for the routine identification of Hypocrea/Trichoderma species and should be useful as a complement to traditional methods...
  57. ncbi Using ITS2 secondary structure to create species-specific oligonucleotide probes for fungi
    Frank C Landis
    Botany Department, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Mycologia 99:681-92. 2007
    ..This combination appears minimally susceptible to false positives and conceivably could be extended to design probes to identify any eukaryotic species...
  58. ncbi 5.8S-28S rRNA interaction and HMM-based ITS2 annotation
    Alexander Keller
    Department of Bioinformatics, University of Wurzburg, Biocenter, Am Hubland, 97074 Wurzburg, Germany
    Gene 430:50-7. 2009
    ..Sequences can be submitted to the following website for HMM-based ITS2 delineation: http://its2.bioapps.biozentrum.uni-wuerzburg.de...
  59. ncbi Shape-shifting corals: molecular markers show morphology is evolutionarily plastic in Porites
    Zac H Forsman
    Hawaii Institute of Marine Biology, Kaneohe, HI 96744, USA
    BMC Evol Biol 9:45. 2009
    ....
  60. ncbi Single integration and spread of a Copia-like sequence nested in rDNA intergenic spacers of Allium cernuum (Alliaceae)
    M Chester
    School of Biological and Chemical Sciences, Queen Mary University of London, London, UK
    Cytogenet Genome Res 129:35-46. 2010
    ..It is likely that units containing the putative retroelement increased in copy number and dispersed via rDNA homogenisation mechanisms, rather than by multiple retrotransposition events...
  61. ncbi Evolution and alignment of the hypervariable arm 1 of Aeschynanthus (Gesneriaceae) ITS2 nuclear ribosomal DNA
    J Denduangboripant
    Institute of Cell and Molecular Biology, The University of Edinburgh, Edinburgh, EH9 3JH, United Kingdom
    Mol Phylogenet Evol 20:163-72. 2001
    ..Alignment based on secondary structure conflicts with our initial manual alignment and, to a lesser extent, with a CLUSTAL X alignment with default parameters...
  62. ncbi Nonuniform concerted evolution and chloroplast capture: heterogeneity of observed introgression patterns in three molecular data partition phylogenies of Asian Mitella (saxifragaceae)
    Yudai Okuyama
    Graduate School of Human and Environmental Studies, Kyoto University, Kyoto, Japan
    Mol Biol Evol 22:285-96. 2005
    ....
  63. ncbi The allopolyploid Arabidopsis kamchatica originated from multiple individuals of Arabidopsis lyrata and Arabidopsis halleri
    Rie Shimizu-Inatsugi
    Institute of Plant Biology, University of Zurich, Zurich CH 8008, Switzerland
    Mol Ecol 18:4024-48. 2009
    ..The haplotypes of low-copy nuclear genes in Japan suggest independent polyploidization events rather than introgression. Our findings suggest that self-compatibility and gene silencing occurred independently in different origins...
  64. ncbi Cooccurring Gentiana verna and Gentiana acaulis and their neighboring plants in two Swiss upper montane meadows harbor distinct arbuscular mycorrhizal fungal communities
    Zuzana Sýkorová
    Institute of Botany, University of Basel, Hebelstrasse 1, CH 4056 Basel, Switzerland
    Appl Environ Microbiol 73:5426-34. 2007
    ..No evidence was found for a narrowing of the mycosymbiont spectrum in the two green gentians, in contrast to previous findings with their achlorophyllous relatives...
  65. ncbi Multiplex PCR for species discrimination of Sclerotiniaceae by novel laccase introns
    Steffen Hirschhäuser
    Institute of Microbiology and Wine Research, Johannes Gutenberg University of Mainz, 55128 Mainz, Germany
    Int J Food Microbiol 118:151-7. 2007
    ..In the present study prevailed problems in the field of a general identification of fungal specimen are highlighted...
  66. ncbi Pythium cryptoirregulare, a new species within the P. irregulare complex
    Carla D Garzón
    Department of Plant Pathology, Pennsylvania State University, University Park, Pennsylvania 16802 4506, USA
    Mycologia 99:291-301. 2007
    ..We propose that a new species, Pythium cryptoirregulare, be delineated from Pythium irregulare sensu stricto...
  67. ncbi Molecular and morphological discrimination of stipitate hydnoids in the genera Hydnellum and Phellodon
    David Parfitt
    Cardiff School of Biosciences, Cardiff University, Cardiff CF10 3TL, UK
    Mycol Res 111:761-77. 2007
    ..Specific PCR primers were constructed to discriminate fruit bodies, mycelium, and mycorrhizal roots of P. niger and P. confluens from each other and from other stipitate hydnoids...
  68. ncbi Detection and identification of wild yeasts in Champús, a fermented Colombian maize beverage
    Esteban Osorio-Cadavid
    Departamento de Biologia, Universidad del Valle, Calle 13 No 100 00, Cali, Colombia
    Food Microbiol 25:771-7. 2008
    ..The Champús strains could thus represent an important source for new yeast biotypes with potential industrial applications...
  69. ncbi Diversity and structure of ectomycorrhizal and co-associated fungal communities in a serpentine soil
    Alexander Urban
    Fungal Genomics Unit, Austrian Research Centers, Tech Gate Vienna, Donau City Strasse 1, 1220 Vienna, Austria
    Mycorrhiza 18:339-54. 2008
    ..Distribution patterns of ECM-co-associated fungi hint at the importance of specific inter-fungal interactions, which are hypothesised to be a relevant factor for the maintenance of ECM diversity...
  70. ncbi Botryosphaeriaceae as potential pathogens of prunus species in South Africa, with descriptions of Diplodia africana and Lasiodiplodia plurivora sp. nov
    Ulrike Damm
    Department of Plant Pathology, University of Stellenbosch, Private Bag X1, Stellenbosch 7602, South Africa
    Mycologia 99:664-80. 2007
    ..nov. from P. salicina and Diplodia africana sp. nov. from P. persica. All species, except Dothiorella viticola, caused lesions on green nectarine and/or plum shoots in a detached shoot pathogenicity assay...
  71. ncbi Molecular identification and phylogenetic analysis of nuclear rDNA sequences among three opisthorchid liver fluke species (Opisthorchiidae: Trematoda)
    Seokha Kang
    Department of Parasitology and Medical Research Institute, College of Medicine, Chungbuk National University, Cheongju, Chungbuk 361 763, Republic of Korea
    Parasitol Int 57:191-7. 2008
    ..Our data are insufficient for discriminating between sudden cladogenesis and other potential causes of poor resolution. Further information from independent loci might help resolve this phylogeny...
  72. ncbi Selection of a set of specific primers for the identification of Tuber rufum: a truffle species with high genetic variability
    Mirco Iotti
    FEMS Microbiol Lett 277:223-31. 2007
    ..Tuber rufum mycorrhiza obtained in the greenhouse using mycelial inoculation techniques had morphological features similar to those of other species of Tuber, stressing the importance of molecular tools for their identification...
  73. ncbi The Peniophorella praetermissa species complex (Basidiomycota)
    Nils Hallenberg
    Department of Plant and Environmental Sciences, Goteborg University, Box 461, S 405 30 Goteborg, Sweden
    Mycol Res 111:1366-76. 2007
    ..However, the geographically most widespread clade with many representatives from both hemispheres is here referred to as P. pertenuis, a taxon that was previously considered a synonym to P. praetermissa...
  74. ncbi Saccharomyces arboricolus sp. nov., a yeast species from tree bark
    Shi an Wang
    Systematic Mycology and Lichenology Laboratory, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China
    Int J Syst Evol Microbiol 58:510-4. 2008
    ..The name Saccharomyces arboricolus sp. nov. was proposed for the novel species, with H-6(T) (=AS 2.3317(T)=CBS 10644(T)) isolated from the bark of Quercus fabri as the type strain...
  75. ncbi Geographic locality and host identity shape fungal endophyte communities in cupressaceous trees
    Michele T Hoffman
    Division of Plant Pathology and Microbiology, Department of Plant Sciences, 1140 East South Campus Drive, University of Arizona, Tucson, AZ 85721, USA
    Mycol Res 112:331-44. 2008
    ..Together, our data show that not only do the abundance, diversity, and taxonomic composition of endophyte communities differ as a function of host identity and locality, but that host affinities of those communities are variable as well...
  76. ncbi Reappraisal of the Sporobolomyces roseus species complex and description of Sporidiobolus metaroseus sp. nov
    Elisabete Valério
    Centro de Recursos Microbiológicos, Departamento de Ciências da Vida, Faculdade de Ciencias e Tecnologia, Universidade Nova de Lisboa, 2829 516 Caparica, Portugal
    Int J Syst Evol Microbiol 58:736-41. 2008
    ..Our studies also led to the clarification of the life cycle of Sporidiobolus pararoseus. We confirm that the teliospores of this species germinate by forming short branches of hyphae, instead of basidia...
  77. ncbi Secondary structure of ITS2 rRNA provides taxonomic characters for systematic studies--a case in Lycoperdaceae (Basidiomycota)
    Dirk Krüger
    The Botany Department, University of Wisconsin, 430 Lincoln Drive, Madison, WI 53706, USA
    Mycol Res 112:316-30. 2008
    ..These consensus structures are illustrated for the clades /Calvatia, /Handkea-Echinatum, /Vascellum, /Morganella, and /Plumbea-Paludosa (Bovista)...
  78. ncbi Polyphasic re-examination of Yarrowia lipolytica strains and the description of three novel Candida species: Candida oslonensis sp. nov., Candida alimentaria sp. nov. and Candida hollandica sp. nov
    Ann Kristin Knutsen
    National Veterinary Institute, Ullevålsveien 68, PO Box 8156 Dep, 0033 Oslo, Norway
    Int J Syst Evol Microbiol 57:2426-35. 2007
    ..nov. (type strain CBS 10151(T) =NRRL Y-48253(T); Mycobank number MB 510770) and Candida hollandica sp. nov. (type strain CBS 4855(T) =NRRL Y-48254(T); Mycobank number MB 510771)...
  79. ncbi Genomic and phenotypic heterogeneity of Acidithiobacillus spp. strains isolated from diverse habitats in China
    Yong Qing Ni
    Key Laboratory of Arid and Grassland Ecology of the Ministry of Education, School of Life Sciences, Lanzhou University, Lanzhou, China
    FEMS Microbiol Ecol 64:248-59. 2008
    ..The results showed that the Chinese Acidithiobacillus spp. isolates exhibited a high degree of genomic and phenotypic heterogeneity...
  80. ncbi Tomentella africana, a new species from Benin (West Africa) identified by morphological and molecular data
    Nourou Soulemane Yorou
    Department Biology I and Geo Bio Centre, LMU, Organismic Biology Mycology, Menzinger Strasse 67, D 80638, Munchen, Germany
    Mycologia 100:68-80. 2008
    ..Differences between irregularly shaped surface thin hyphae and skeletal ones are highlighted. We stress the relevance of rhizomorphal structures in the discrimination of resupinate thelephoroid fungi...
  81. ncbi Molecular diversity and intragenomic variability in the yeast genus Xanthophyllomyces: the origin of Phaffia rhodozyma?
    Jack W Fell
    Rosenstiel School of Marine and Atmospheric Science, University of Miami, Key Biscayne, FL, USA
    FEMS Yeast Res 7:1399-408. 2007
    ..rhodozyma (CBS 5905(T), UCD 67-210(T)) was derived as a mating-deficient basidiospore from the parent teleomorphic strain CBS 9090...
  82. ncbi PCR-based determination of internal transcribed spacer (ITS) regions of ribosomal DNA of giant intestinal fluke, Fasciolopsis buski (Lankester, 1857) Looss, 1899
    P K Prasad
    Department of Zoology, North Eastern Hill University, Shillong, 793022 Meghalaya, India
    Parasitol Res 101:1581-7. 2007
    ..The results corroborate that the ITS sequences are not stage specific and are conserved through different stages of development of the fluke, and thus could be used as species markers...
  83. ncbi Taxonomy of displaced species of Tubaria
    P Brandon Matheny
    Clark University, Biology Department, 950 Main Street, Worcester, Massachusetts 01610, USA
    Mycologia 99:569-85. 2007
    ..Four new combinations are proposed, and one new species, T. bispora, is described as new from Costa Rica and Martinique. A lectotype is designated for Pholiota serrulata...
  84. ncbi Erysiphe abbreviata on cherry bark oak--morphology, phylogeny and taxonomy
    Uwe Braun
    Institute of Biology, Geobotany and Botanical Garden, Herbarium, Martin Luther University, Neuwerk 21, D 06099 Halle, Germany
    Mycologia 99:655-63. 2007
    ..The structure of the anamorph (Oidium subgen. Pseudoidium) and the molecular data support the placement of this species in Erysiphe emend. (including Microsphaera) as a species separate from the Eurasian Erysiphe alphitoides...
  85. ncbi An analysis of inter- and intraspecific genetic variabilities in the Kluyveromyces marxianus group of yeast species for the reconsideration of the K. lactis taxon
    Carmela Belloch
    , Universitat de Valencia, Calle Dr Moliner 50, 46100 Burjassot, Valencia, Spain
    Yeast 19:257-68. 2002
    ..These results suggest that the present taxon K. lactis is a complex of different species, subspecies or, at least, genetically structured populations...
  86. ncbi Two new ballistoconidium-forming yeast species, Bullera melastomae and Bullera formosana, found in Taiwan
    Takashi Nakase
    Japan Collection of Microorganisms, RIKEN The Institute of Physical and Chemical Research, Wako, Saitama, Japan
    Syst Appl Microbiol 27:558-64. 2004
    ..nov. and Bullera formosana sp. nov. In the phylogenetic trees based on 18S rDNA and D1/D2 domain of 26S rDNA sequences, these two species constitute a cluster connected with Dioszegia cluster in the Cryptococcus luteolus lineage...
  87. ncbi Candida asparagi sp. nov., Candida diospyri sp. nov. and Candida qinlingensis sp. nov., novel anamorphic, ascomycetous yeast species
    Hui-Zhong Lu
    Systematic Mycology and Lichenology Laboratory, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China
    Int J Syst Evol Microbiol 54:1409-14. 2004
    ..nov. (type strain, QL 21-2T=AS 2.2525T=CBS 9769T) are Candida friedrichii and Candida membranifaciens. Candida asparagi sp. nov. (type strain, SN 15-1T=AS 2.2526T=CBS 9770T) forms a clade with Candida fructus...
  88. ncbi Curvibasidium cygneicollum gen. nov., sp. nov. and Curvibasidium pallidicorallinum sp. nov., novel taxa in the Microbotryomycetidae (Urediniomycetes), and their relationship with Rhodotorula fujisanensis and Rhodotorula nothofagi
    José Paulo Sampaio
    Centro de Recursos Microbiológicos, Secção Autónoma de Biotecnologia, Faculdade de Ciencias e Tecnologia, Universidade Nova de Lisboa, 2829 516 Caparica, Portugal
    Int J Syst Evol Microbiol 54:1401-7. 2004
    ..nov. (CBS 4551T), which is described as the sexual stage of R. fujisanensis, and Curvibasidium pallidicorallinum sp. nov. (CBS 9091T), which is related closely to R. nothofagi, but does not represent its sexual stage...
  89. ncbi Characterisation of wild legume nodulating bacteria (LNB) in the infra-arid zone of Tunisia
    Frédéric Zakhia
    Laboratoire des Symbioses Tropicales et Méditerranéennes, IRD INRA CIRAD AGRO M UM II, Montpellier, France
    Syst Appl Microbiol 27:380-95. 2004
    ..orientalis in northern regions so far...
  90. ncbi PCR-based molecular discrimination of Pandora neoaphidis isolates from related entomopathogenic fungi and development of species-specific diagnostic primers
    Anna M Tymon
    Plant and Invertebrate Ecology Division, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK
    Mycol Res 108:419-33. 2004
    ..The primers are useful tools for quantifying the epizootiology of P. neoaphidis in aphid populations, as well as assessing competitive interactions between these two species...
  91. ncbi Polymorphisms in nuclear rDNA and mtDNA reveal the polyphyletic nature of isolates of Phomopsis pathogenic to sunflower and a tight monophyletic clade of defined geographic origin
    Djaouida Rekab
    Dipartimento di Biologia Applicata alla Difesa delle Piante, , via Scienze 208, Udine 33100, Italy
    Mycol Res 108:393-402. 2004
    ....
  92. ncbi Development of PCR assays for the detection and differentiation of Fusarium sporotrichioides and Fusarium langsethiae
    Anthony Wilson
    Department of Metabolic Biology, John Innes Centre, Norwich Research Park, Colney Lane, Norwich NR4 7UH, UK
    FEMS Microbiol Lett 233:69-76. 2004
    ..These assays were able to detect both species in samples of grain taken from the field...
  93. ncbi Genetic diversity of bradyrhizobial populations from diverse geographic origins that nodulate Lupinus spp. and Ornithopus spp
    Adriana Jarabo-Lorenzo
    , Universidad de La Laguna, Spain
    Syst Appl Microbiol 26:611-23. 2003
    ....
  94. ncbi The taxonomic position of Saccharomyces boulardii as evaluated by sequence analysis of the D1/D2 domain of 26S rDNA, the ITS1-5.8S rDNA-ITS2 region and the mitochondrial cytochrome-c oxidase II gene
    Alis van der Aa Kühle
    Department of Dairy and Food Science, Food Microbiology, The Royal Veterinary and Agricultural University, Copenhagen, Denmark
    Syst Appl Microbiol 26:564-71. 2003
    ..The results of the present study strongly indicate a close relatedness of S. boulardii to S. cerevisiae and thereby support the recognition of S. boulardii as a member of S. cerevisiae and not as a separate species...
  95. ncbi Identification of Carnobacterium, Lactobacillus, Leuconostoc and Pediococcus by rDNA-based techniques
    E Chenoll
    , Universitat de Valencia, Spain
    Syst Appl Microbiol 26:546-56. 2003
    ..It was necessary to combine profiles generated by restriction of ISR-DdeI, ARDRA-DdeI and ARDRA-HaeIII in order to complete the identification of Leuconostoc species...
  96. ncbi Ribosomal DNA variation, recombination and inheritance in the basidiomycete Trichaptum abietinum: implications for reticulate evolution
    H Kauserud
    Division of Botany and Plant Physiology, Department of Biology, University of Oslo, PO Box 1045, Blindern, N 0316 Oslo, Norway
    Heredity (Edinb) 91:163-72. 2003
    ..The results are discussed in the light of other evolutionary mechanisms as well...
  97. ncbi ITS-RFLP and ITS sequence analysis of a foliar endophytic Phyllosticta from different tropical trees
    Ajay K Pandey
    Department of Biotechnology, Thapar Institute of Engineering and Technology, Patiala 147 001, India
    Mycol Res 107:439-44. 2003
    ..capitalensis. These results show that P. capitalensis (teleomorph Guignardia endophyllicola?) is an ubiquitous foliar endophyte that can infect tree hosts from different families and habitats...
  98. ncbi Genetic relatedness among Filobasidiella species
    Swarna Sivakumaran
    School of Biological and Chemical Sciences, Birkbeck College, London WC1E 7HX, UK
    Mycopathologia 156:157-62. 2002
    ..F. lutea was recovered as a distinct, but closely related taxon with the Filobasidiella clade. This is the first report of DNA sequences from herbarium specimens of F. lutea...
  99. ncbi Ectomycorrhizas involving sebacinoid mycobionts
    Alexander Urban
    Universitat Wien, Institut fur Botanik, Abteilung Mykologie und Kryptogamen, Rennweg 14, A 1030 Wien, Austria
    Mycol Res 107:3-14. 2003
    ..Observations on ectomycorrhizas and basidiomes suggest that species of Sebacinaceae are fairly common mycobionts in various ectomycorrhizal plant communities...
  100. ncbi Mycorrhizal and root endophytic fungi of containerized Picea glauca seedlings assessed by rDNA sequence analysis
    G Kernaghan
    Department of Renewable Resources, University of Alberta, Edmonton, Alberta, Canada T6G 2H1
    Microb Ecol 45:128-36. 2003
    ..Correspondence analysis revealed strong positive and negative associations among fungal taxa as well as an influence of applied fertilizer level on fungal diversity and species composition...
  101. ncbi Detection and taxonomic placement of endophytic fungi within frond tissues of Livistona chinensis based on rDNA sequences
    L D Guo
    Centre for Research in Fungal Diversity, Department of Ecology and Biodiversity, The University of Hong Kong, Pokfulam Road, Hong Kong, China
    Mol Phylogenet Evol 20:1-13. 2001
    ..The direct approach to detection and taxonomic placement of endophytic fungi within host tissue without the need for conventional in vitro culturing is discussed...

Research Grants48

  1. Evaluating Trichophyton tonsurans Carriage and Infection
    Susan Abdel Rahman; Fiscal Year: 2006
    ..abstract_text> ..
  2. FUNGAL PROTEIN/GLYCOPROTEIN EXPRESSION IN TINEA CAPITIS
    Susan Abdel Rahman; Fiscal Year: 2003
    ..abstract_text> ..
  3. High through-put detection of fungal pathogens
    JACK FELL; Fiscal Year: 2004
    ..Resulting data will be disseminated via GenBank, publications in leading scientific journals and as an inexpensive commercial detection system. ..
  4. Genetics of Cryptosporidium parvum
    Giovanni Widmer; Fiscal Year: 2006
    ..We will test the following two working hypotheses: a) Virulence is determined by multiple genetic loci. b) QTL analysis can be used to identify genomic segments associated with virulence. ..
  5. A Genome Sequence Survey of Enterocytozoon bieneusi
    Donna Akiyoshi; Fiscal Year: 2006
    ..c. Establishment of a website to provide access to the sequence data and clones by the scientific community. ..
  6. LARVAL MOSQUITO FEEDING AND MICROBIAL INTERACTIONS
    Edward Walker; Fiscal Year: 2006
    ..3) Studies of transformant efficacy against mosquito larvae, and persistence in simulated mosquito habitats. ..
  7. Role of non-tuberculous mycobacteria in lung disease
    Jason Stout; Fiscal Year: 2006
    ..Our findings will also assist clinicians in predicting which patients with NTM isolated from the respiratory tract will have NTM pulmonary disease. ..
  8. Microbial Mediation of Habitat Selection and Production
    Edward Walker; Fiscal Year: 2006
    ..Microbial basis for larval habitat production will be studied with field and lab experiments where nutrient sources and microbial communities are quantified, manipulated and larval growth responses and adult production quantified. ..
  9. Subjective Symptoms after Treatment of Lyme Disease
    Gary P Wormser; Fiscal Year: 2010
    ..In a different group of patients, we will describe for the first time the outcome of patients with early Lyme disease more than 10 years after diagnosis and treatment. ..
  10. Insecticide mosaics and sustainability of treated nets
    Edward Walker; Fiscal Year: 2007
    ..Industry partners Vestergaard-Frandsen and Syngenta are directly involved in these efforts, for formulation and evaluation purposes. ..
  11. Population genetic structure and bionomics of Anopheles lesteri in China
    Yajun Ma; Fiscal Year: 2007
    ..lesteri mosquitoes as well as the geographic variation of its bionomics. This information is particularly useful for the design of rational area-specific vector control methods. ..
  12. Genetics of Cryptosporidium parvum
    Giovanni Widmer; Fiscal Year: 2010
    ..Such valleys will be identified by quantitatively genotyping a selected and a control progeny population with a panel of single nucleotide polymorphisms. ..
  13. Microbial Correlates of Bacterial Vaginosis Treatment Failure and Recurrence in H
    Michael J Ferris; Fiscal Year: 2010
    ....
  14. LARVAL MOSQUITO FEEDING AND MICROBIAL INTERACTIONS
    Edward Walker; Fiscal Year: 2007
    ..abstract_text> ..
  15. Topical Antibiotics to Prevent Lyme Disease
    Gary Wormser; Fiscal Year: 2007
    ....
  16. Functional Genomics of Cryptosporidium parvum
    Giovanni Widmer; Fiscal Year: 2007
    ..parvum. A comparison of the host cell response to C. parvum type 1 and type 2 will be performed to probe the molecular basis of the observed difference in virulence between types. ..
  17. Genetic Transformation of Cryptosporidium parvum
    Giovanni Widmer; Fiscal Year: 2005
    ..Approach: Transformation by electroporation of sporozoites with GFP expression plasmids; detection of transformants in cell culture by fluorescent microscopy, anti-GFP antibodies, or reverse-transcriptase PCR. ..
  18. LARVAL MOSQUITO FEEDING AND MICROBIAL INTERACTIONS
    Edward Walker; Fiscal Year: 2001
    ..Finally, we aim to introduce recombinants into laboratory microcosm habitats and examine the persistence of these organisms by utilizing drug resistance markers and PCR-based detection of the cryD gene fragment. ..
  19. LARVAL MOSQUITO FEEDING AND MICROBIAL INTERACTIONS
    Edward Walker; Fiscal Year: 1993
    ....
  20. Mark I Model 68A Rodent/Cell line Gamma Irradiator
    Sanford Feldman; Fiscal Year: 2004
    ....