Genomes and Genes
ribosomal spacer dna
Summary: The intergenic DNA segments that are between the ribosomal RNA genes (internal transcribed spacers) and between the tandemly repeated units of rDNA (external transcribed spacers and nontranscribed spacers).
Publications254 found, 100 shown here
- Ribosomal ITS sequences and plant phylogenetic inferenceI Alvarez
Department of Botany, Iowa State University, Ames, IA 50011, USA
Mol Phylogenet Evol 29:417-34. 2003..It is suggested that more robust insights are likely to emerge from the use of single-copy or low-copy nuclear genes...
- Pan-eukaryote ITS2 homologies revealed by RNA secondary structureAnnette W Coleman
Division of Biology and Medicine, Brown University, Providence, RI 02912, USA
Nucleic Acids Res 35:3322-9. 2007..Equally important, the recognition of eukaryote-wide homology regions provides extensive and detailed information to test experimental studies of ribosomal rRNA processing...
- Evolution of the secondary structure of the rRNA internal transcribed spacer 2 (ITS2) in hard ticks (Ixodidae, Arthropoda)O Hlinka
Department of Microbiology and Parasitology, and Institute for Molecular Biosciences, The University of Queensland, Brisbane 4072, Australia
Heredity (Edinb) 88:275-9. 2002....
- Phylogenetic relationships and evolutionary traits in Ranunculus s.l. (Ranunculaceae) inferred from ITS sequence analysisElvira Hörandl
Department of Systematic and Evolutionary Botany, Faculty of Life Sciences, University of Vienna, Rennweg 14, A 1030 Vienna, Austria
Mol Phylogenet Evol 36:305-27. 2005....
- Molecular phylogeny and biogeography of the widely distributed Amanita species, A. muscaria and A. pantherinaTakashi Oda
Laboratory of Environmental Mycoscience, Graduate School of Agriculture, Kyoto University, Kyoto, 606 8502, Japan
Mycol Res 108:885-96. 2004..For both A. muscaria and A. pantherina, the intracontinental relationships of both Eurasia and North America were closer than the relationships between eastern Asia and eastern North America...
- The use of ITS1 rDNA PCR in detecting pathogenic African trypanosomesZ K Njiru
Division of Veterinary and Biomedical Sciences, Western Australian Biomedical Research Institute WABRI, Murdoch University, South Street, 6150, Murdoch, Western Australia, Australia
Parasitol Res 95:186-92. 2005..congolense savannah 30/33 (90.9%) but was lower with T. vivax 25/31 (77.4%). The primers offer promise as a routine diagnostic tool through the use of a single PCR; however, further evaluation is recommended...
- A two-locus global DNA barcode for land plants: the coding rbcL gene complements the non-coding trnH-psbA spacer regionW John Kress
Department of Botany, National Museum of Natural History, Smithsonian Institution, Washington, DC, United States of America
PLoS ONE 2:e508. 2007..This low rate has complicated the trade-off in finding a locus that is universal and readily sequenced and has sufficiently high sequence divergence at the species-level...
- A survey of nuclear ribosomal internal transcribed spacer substitution rates across angiosperms: an approximate molecular clock with life history effectsKathleen M Kay
Department of Plant Biology, Michigan State University, 166 Plant Biology Building, East Lansing, MI 48824, USA
BMC Evol Biol 6:36. 2006..Here, we systematically review the literature to assess substitution rate variation in nrITS among angiosperms, and we evaluate possible correlates of the variation...
- A common core of secondary structure of the internal transcribed spacer 2 (ITS2) throughout the EukaryotaJörg Schultz
Department of Bioinformatics, University of Wurzburg, Biocenter, Am Hubland, D 97074 Wurzburg, Germany
RNA 11:361-4. 2005..In summary, our analysis strengthens the potential of ITS2 as a general phylogenetic marker and provides a data source for further ITS2-based analyses...
- Multifarious characterization of leishmania tropica from a Judean desert focus, exposing intraspecific diversity and incriminating phlebotomus sergenti as its vectorLionel F Schnur
Department of Parasitology, The Kuvin Center for the Study of Infectious and Tropical Diseases, Hebrew University Hadassah Medical School, Jerusalem, Israel
Am J Trop Med Hyg 70:364-72. 2004..tropica strains into two clusters that were not entirely congruent with geographic distribution. These results support the heterogeneous nature of L. tropica and incriminate Ph. sergenti as its vector in this Judean Desert focus...
- A Beauveria phylogeny inferred from nuclear ITS and EF1-alpha sequences: evidence for cryptic diversification and links to Cordyceps teleomorphsStephen A Rehner
Insect Biocontrol Laboratory, USDA ARS, Beltsville, Maryland 20705, USA
Mycologia 97:84-98. 2005..These results establish a phylogenetic framework for further taxonomic, phylogenetic and comparative biological investigations of Beauveria and their corresponding Cordyceps teleomorphs...
- European Lymnaeidae (Mollusca: Gastropoda), intermediate hosts of trematodiases, based on nuclear ribosomal DNA ITS-2 sequencesM D Bargues
, Facultad de Farmacia, Universidad de Valencia, , 46100 Burjassot, Valencia, Spain
Infect Genet Evol 1:85-107. 2001..Similarly, ITS-2 sequencing would allow a step forward in the appropriate rearrangement of the actual systematic confusion among echinostomatids...
- ITS secondary structure derived from comparative analysis: implications for sequence alignment and phylogeny of the AsteraceaeLeslie R Goertzen
Section of Integrative Biology and Institute of Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA
Mol Phylogenet Evol 29:216-34. 2003..Combined analyses of ndhF and ITS sequences provide additional resolution and support for relationships in the family...
- Presumptive horizontal symbiont transmission in the fungus-growing termite Macrotermes natalensisH H de Fine Licht
Department of Population Biology, Institute of Biology, University of Copenhagen, Universitetsparken 15, 2100 Copenhagen, Denmark
Mol Ecol 15:3131-8. 2006..natalensis mounds puzzling. To our knowledge, this is the first detailed study of the genetic population structure of a single lineage of Termitomyces...
- Termitomyces sp. associated with the termite Macrotermes natalensis has a heterothallic mating system and multinucleate cellsHenrik H De Fine Licht
Department of Population Biology, Biological Institute, University of Copenhagen, DK 2100 Copenhagen O, Denmark
Mycol Res 109:314-8. 2005..This is the first time the mating system of a Termitomnyces species has been studied...
- Intragenomic length variation of the ribosomal DNA intergenic spacer in a malaria vector, Anopheles sinensisIn Jung Whang
School of Biological Sciences, Seoul National University, Korea
Mol Cells 14:158-62. 2002..This suggestion is far from being conclusive at present, but a further genetic study will bring more compelling evidences for this pending issue...
- Inferring the history of the polyploid Silene aegaea (Caryophyllaceae) using plastid and homoeologous nuclear DNA sequencesM Popp
Department of Systematic Botany, Uppsala University, Uppsala, SE-752 36, Sweden
Mol Phylogenet Evol 20:474-81. 2001..pentelica as the maternal lineage. The strength of the approach of using homoeologous sequences of several loci is demonstrated, and its usefulness for the study of phylogenies of groups including polyploids is emphasized...
- Variation in 16S-23S rRNA intergenic spacer regions in Photobacterium damselae: a mosaic-like structureCarlos R Osorio
Departamento de Microbioloxía e Parasitoloxía e Instituto de Acuicultura, Universidade de Santiago de Compostela, Campus Sur, 15782 Santiago de Compostela, Galicia, Spain
Appl Environ Microbiol 71:636-45. 2005..The main source of variation between ITS variants was due to different combinations of DNA sequence blocks, constituting a mosaic-like structure...
- ITS 2 sequences heterogeneity in Phlebotomus sergenti and Phlebotomus similis (Diptera, Psychodidae): possible consequences in their ability to transmit Leishmania tropicaJerome Depaquit
Laboratoire de Parasitologie, Faculte de Pharmacie, 51 rue Cognacq Jay, 51096 Reims Cedex, France
Int J Parasitol 32:1123-31. 2002..Two groups can be individualised, one oriental and one western in connection with ecology, host preferences and distribution of L. tropica. We hypothesise that they could be correlated with differences in vectorial capacities...
- Phylogenetic relationships among plant and animal parasites, and saprotrophs in Aphanomyces (Oomycetes)Javier Diéguez-Uribeondo
Real Jardín Botánico CSIC, Madrid, Spain
Fungal Genet Biol 46:365-76. 2009..Saprotrophic or opportunistic species formed a separate evolutionary lineage except Aphanomyces stellatus whose evolutionary position has not yet been resolved...
- Homology modeling revealed more than 20,000 rRNA internal transcribed spacer 2 (ITS2) secondary structuresMatthias Wolf
Department of Bioinformatics, University of Wurzburg, Biocenter, Am Hubland, Germany
RNA 11:1616-23. 2005..bioapps.biozentrum.uni-wuerzburg.de...
- Sir2 represses endogenous polymerase II transcription units in the ribosomal DNA nontranscribed spacerChonghua Li
Department of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77843, USA
Mol Biol Cell 17:3848-59. 2006....
- Characteristics of the nuclear (18S, 5.8S, 28S and 5S) and mitochondrial (12S and 16S) rRNA genes of Apis mellifera (Insecta: Hymenoptera): structure, organization, and retrotransposable elementsJ J Gillespie
Department of Entomology, Texas A and M University, College Station, TX, USA
Insect Mol Biol 15:657-86. 2006....
- SSCP-based identification of members within the Pseudoterranova decipiens complex (Nematoda: Ascaridoidea: Anisakidae) using genetic markers in the internal transcribed spacers of ribosomal DNAX Q Zhu
Department of Veterinary Science, The University of Melbourne, Werribee, Victoria, Australia
Parasitology 124:615-23. 2002....
- Why nuclear ribosomal DNA spacers (ITS) tell different stories in QuercusM Mayol
, , Bellaterra, Barcelona, E-08193, Spain
Mol Phylogenet Evol 19:167-76. 2001....
- 16S-23S rDNA internal transcribed spacer sequences for analysis of the phylogenetic relationships among species of the genus FusobacteriumGeorg Conrads
R M Alden Research Laboratory, Santa Monica University of California at Los Angeles Medical Center, Santa Monica 90404, USA
Int J Syst Evol Microbiol 52:493-9. 2002..Fusobacterium necrophorum subspp. necrophorum and funduliforme on the one hand, and Fusobacterium varium and Fusobacterium mortiferum on the other, were found to be very similar, even at the high-resolution ITS level...
- ITS2 rRNA evolution and its congruence with the phylogeny of muricid neogastropods (Caenogastropoda, Muricoidea)Marco Oliverio
Dipartimento di Biologia Animale e dell Uomo, La Sapienza University, Viale dell Università 32, I 00185 Roma, Italy
Mol Phylogenet Evol 25:63-9. 2002
- Phylogenetic relationships in Termitomyces (Family Agaricaceae) based on the nucleotide sequence of ITS: a first approach to elucidate the evolutionary history of the symbiosis between fungus-growing termites and their fungiCorinne Rouland-Lefevre
Laboratoire d Ecophysiologie des Invertébrés, Université Paris XII Val de Marne, Creteil Cedex, 94010, France
Mol Phylogenet Evol 22:423-9. 2002....
- Marked intragenomic heterogeneity and geographical differentiation of nrDNA ITS in Larix potaninii (Pinaceae)Xiao Xin Wei
Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, 20 Nanxincun, Xiangshan, Beijing 100093, China
J Mol Evol 57:623-35. 2003..In addition, some varieties of L. potaninii might have experienced an nrDNA founder effect parallel to their geographical isolation...
- Diversity and phylogenetic affinities of foliar fungal endophytes in loblolly pine inferred by culturing and environmental PCRA Elizabeth Arnold
Department of Biology, Duke University, Durham, North Carolina 27708, USA
Mycologia 99:185-206. 2007....
- Slow algae, fast fungi: exceptionally high nucleotide substitution rate differences between lichenized fungi Omphalina and their symbiotic green algae CoccomyxaStefan Zoller
Department of Biology, Duke University, Durham, NC 27708 0338, USA
Mol Phylogenet Evol 29:629-40. 2003....
- Rapid diversification of a species-rich genus of neotropical rain forest treesJ E Richardson
Royal Botanic Garden Edinburgh, 20a Inverleith Row, Edinburgh EH3 5LR, UK
Science 293:2242-5. 2001..Inga may be representative of other species-rich neotropical genera with rapid growth and reproduction, which contribute substantially to species numbers in the world's most diverse flora...
- Sequence comparison of distal and proximal ribosomal DNA arrays in rice (Oryza sativa L.) chromosome 9S and analysis of their flanking regionsMasaki Fujisawa
Rice Genome Research Program, National Institute of Agrobiological Sciences Institute of the Society for Techno innovation of Agriculture, Forestry and Fisheries, Tsukuba, Ibaraki, 305 8602, Japan
Theor Appl Genet 113:419-28. 2006..This study reveals the repetitive nature of the telomeric end of the short arm of chromosome 9, which consists of telomeric repeats, an rDNA array, and a retrotransposon-rich chromosomal region...
- The internal transcribed spacer of nuclear ribosomal DNA in the gymnosperm GnetumHyosig Won
Department of Biology, University of Missouri St Louis, St Louis, MO 63121, USA
Mol Phylogenet Evol 36:581-97. 2005..Based on morphology and chromosome numbers, the most plausible causes of the observed high levels of ITS polymorphism are hybridization, allopolyploidy, and introgression...
- Development and evaluation of a species diagnostic polymerase chain reaction-restriction fragment-length polymorphism procedure for cryptic members of the Culex sitiens (Diptera: Culicidae) subgroup in Australia and the southwest PacificN W Beebe
Molecular Parasitology Unit, University of Technology, Sydney, New South Wales, Australia
J Med Entomol 39:362-9. 2002..Overlapping adult morphology was observed for Cx. annulirostris and Cx. palpalis in both northern Queensland and PNG and for all three species at one site in northwest Queensland...
- Single integration and spread of a Copia-like sequence nested in rDNA intergenic spacers of Allium cernuum (Alliaceae)M Chester
School of Biological and Chemical Sciences, Queen Mary University of London, London, UK
Cytogenet Genome Res 129:35-46. 2010..It is likely that units containing the putative retroelement increased in copy number and dispersed via rDNA homogenisation mechanisms, rather than by multiple retrotransposition events...
- Inter- and intraspecies variations of the 16S-23S rDNA intergenic spacer region of various streptococcal speciesA A Hassan
Institut für Tierärztliche Nahrungsmittelkunde, Professur für Milchwissenschaften der Justus Liebig Universität Giessen, Giessen, Germany
Syst Appl Microbiol 26:97-103. 2003..In addition the size and sequence variations of ISR of S. equi subsp. zooepidemicus, S. porcinus and S. suis allows a molecular typing of isolates of these species possibly useful in epidemiological aspects...
- Niche partitioning of closely related symbiotic dinoflagellatesEugenia M Sampayo
Centre for Marine Studies, The University of Queensland, St Lucia, QLD 4072, Australia
Mol Ecol 16:3721-33. 2007..This study provides thus evidence that closely related symbionts are ecologically distinct and fulfil their own niche within the ecosystem provided by the host and external environment...
- Unusual compact rDNA gene arrangements within some members of the Ascomycota: evidence for molecular co-evolution between ITS1 and ITS2Georg Hausner
Department of Microbiology, University of Mannitoba, Winnipeg, Canada
Genome 48:648-60. 2005..This supports biochemical evidence indicating that the ITS1 and ITS2 segments interact to facilitate the maturation of the rRNA precursor...
- ITS2 sequence-structure analysis in phylogenetics: a how-to manual for molecular systematicsJörg Schultz
Department of Bioinformatics, Biocenter, University of Wurzburg, Am Hubland, D 97074 Wurzburg, Germany
Mol Phylogenet Evol 52:520-3. 2009..Moreover, compensatory base changes (CBCs) in ITS2 sequence-structure pairs are identified as a possible marker for distinguishing species...
- Genetic diversity and molecular identification of mosquito species in the Anopheles maculatus group using the ITS2 region of rDNAC Walton
Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, UK
Infect Genet Evol 7:93-102. 2007..The lack of detectable genetic structure also suggests that populations of these species are either connected by gene flow and/or share a recent common history...
- An oligonucleotide barcode for species identification in Trichoderma and HypocreaIrina S Druzhinina
Division of Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Getreidemarkt 9 1665, A 1060 Vienna, Austria
Fungal Genet Biol 42:813-28. 2005..We conclude that oligonucleotide barcode is a powerful tool for the routine identification of Hypocrea/Trichoderma species and should be useful as a complement to traditional methods...
- UNITE: a database providing web-based methods for the molecular identification of ectomycorrhizal fungiUrmas Kõljalg
Institute of Botany, University of Tartu, 40 Lai Str, EE 51005 Tartu, Estonia
New Phytol 166:1063-8. 2005..It should be noted that the current version of UNITE is dedicated to the reliable identification of ECM fungi. The UNITE database is accessible through the URL http://unite.zbi.ee..
- Paramecium aurelia revisitedA W Coleman
Division of Biology and Medicine, Brown University, Providence, Rhode Island 02912, USA
J Eukaryot Microbiol 52:68-77. 2005....
- Characterisation of Fasciola species from Mainland China by ITS-2 ribosomal DNA sequenceW Y Huang
Department of Veterinary Medicine, College of Animal Science and Technology, Guangxi University, Nanning 530004, Guangxi Zhuang Nationality Autonomous Region, PR China
Vet Parasitol 120:75-83. 2004..This assay should provide a valuable tool for the molecular identification and for studying the ecology and population genetic structures of Fasciola spp. from Mainland China and elsewhere...
- Reproductive isolation among sympatric cryptic species in marine diatomsAlberto Amato
Stazione Zoologica A Dohrn, Villa Comunale, 80121 Napoli, Italy
Protist 158:193-207. 2007..Our findings not only show that cryptic genetic variants abound in sympatry, but also that they are reproductively isolated and, therefore, biologically distinct units...
- ITS2 data corroborate a monophyletic chlorophycean DO-group (Sphaeropleales)Alexander Keller
Department of Bioinformatics, University of Wurzburg, Am Hubland, 97074 Wurzburg, Germany
BMC Evol Biol 8:218. 2008....
- Intragenomic variation of the rDNA internal transcribed spacers in sponges (Phylum Porifera): implications for phylogenetic studiesGert Worheide
Geowissenschaftliches Zentrum, Abt Geobiologie, Universitat Gottingen, Goldschmidtstr 3, D 37077 Gottingen, Germany
Mol Phylogenet Evol 33:816-30. 2004....
- Spectrum of clinically relevant Exophiala species in the United StatesJ S Zeng
Centraalbureau voor Schimmelcultures, NL 3508 AD, Utrecht, The Netherlands
J Clin Microbiol 45:3713-20. 2007..Since relatively few unknown ITS motifs were encountered, we suppose that the list of opportunistic Exophiala species in temperate climates is nearing completion, but a number of species still have to be described...
- ITS-2 and 18S rRNA gene phylogeny of Aplysinidae (Verongida, Demospongiae)Susanne Schmitt
Research Center for Infectious Diseases, University of Wurzburg, Germany
J Mol Evol 60:327-36. 2005..The species concept for Aplysina sponges as well as a phylogenetic history with a possibly Tethyan origin is discussed...
- A PCR based assay for detection and differentiation of African trypanosome species in bloodAndrew Cox
Centre for Tropical Veterinary Medicine, Royal Dick School of Veterinary Medicine, University of Edinburgh, Easter Bush, Roslin, Midlothian EH25 9RG, Scotland, UK
Exp Parasitol 111:24-9. 2005..The method is more economical, simple, and sensitive than current screening methods, and yields more detailed information, thereby making it a viable tool for large-scale epidemiological studies...
- Development of a DNA microarray for detection and identification of fungal pathogens involved in invasive mycosesDirk M Leinberger
Institute of Technical Biochemistry, University of Stuttgart, Allmandring 31, Stuttgart 70569, Germany
J Clin Microbiol 43:4943-53. 2005..As the microarray was able to detect and clearly identify the fungal pathogens within 4 h after DNA extraction, this system offers an interesting potential for clinical microbiology laboratories...
- Molecular phylogenies of fig wasps: partial cocladogenesis of pollinators and parasitesC Lopez-Vaamonde
Department of Biology and NERC Centre for Population Biology, Imperial College, Silwood Park, Ascot, Berkshire SL5 7PY, United Kingdom
Mol Phylogenet Evol 21:55-71. 2001..There is perfect correspondence between two terminal clades of two sister species in the respective phylogenies, and rates of molecular evolution in these pairs are similar...
- Recent origin and phylogenetic utility of divergent ITS putative pseudogenes: a case study from Naucleeae (Rubiaceae)Sylvain G Razafimandimbison
Department of Systemic Biology, Evolutionary Biology Centre, Uppsala University, Sweden
Syst Biol 53:177-92. 2004..fagifolia and none from H. cordifolia and M. rubrostipulata, which suggests that PCR selection is occurring and/or the presumed functional alleles are located at minor loci (with few ribosomal DNA copies)...
- General functions to transform associate data to host data, and their use in phylogenetic inference from sequences with intra-individual variabilityMarkus Göker
Organismic Botany, Eberhard Karls University, Auf der Morgenstelle 1, Tubingen, Germany
BMC Evol Biol 8:86. 2008....
- Ectomycorrhiza of Kobresia myosuroides at a primary successional glacier forefrontOliver Mühlmann
Institute of Microbiology, University Innsbruck, Technikerstr 25, 6020 Innsbruck, Austria
Mycorrhiza 18:355-62. 2008..Lecythophora sp., and one Pezizales species. Due to high interannual differences in the EM fungal community, no significant seasonal changes could be detected. The importance of fungal mycobionts in alpine habitats is discussed...
- Diversity and structure of ectomycorrhizal and co-associated fungal communities in a serpentine soilAlexander Urban
Fungal Genomics Unit, Austrian Research Centers, Tech Gate Vienna, Donau City Strasse 1, 1220 Vienna, Austria
Mycorrhiza 18:339-54. 2008..Distribution patterns of ECM-co-associated fungi hint at the importance of specific inter-fungal interactions, which are hypothesised to be a relevant factor for the maintenance of ECM diversity...
- Phylogenetic reconstruction using secondary structures of Internal Transcribed Spacer 2 (ITS2, rDNA): finding the molecular and morphological gap in Caribbean gorgonian coralsAlejandro Grajales
Departamento de Ciencias Biologicas Facultad de Ciencias, Laboratorio Biología Molecular Marina BIOMMAR, Universidad de Los Andes, Bogota, Colombia
BMC Evol Biol 7:90. 2007..The search for new phylogenetic approaches, such as by RNA secondary structure analysis, is therefore a priority in octocoral research...
- Phylogenetic hypotheses of gorgoniid octocorals according to ITS2 and their predicted RNA secondary structuresCatalina Aguilar
Laboratorio de Biología Molecular Marina BIOMMAR, Departamento de Ciencias Biologicas, Universidad de Los Andes, Bogota, Colombia
Mol Phylogenet Evol 43:774-86. 2007..DNA sequences from the nuclear ITS2 region, including information from predicted RNA secondary structure, despite their reduced length, provided numerous characters and phylogenetic information among Gorgoniidae genera and species...
- Recent natural hybridization between two allopolyploid wheatgrasses (Elytrigia, Poaceae): ecological and evolutionary implicationsVáclav Mahelka
Institute of Botany, Academy of Sciences of the Czech Republic, Zámek 1, CZ 25243, Pruhonice, Czech Republic
Ann Bot 100:249-60. 2007..Natural hybridization was investigated between two predominantly allohexaploid wheatgrasses, weedy Elytrigia repens and steppic E. intermedia, with respect to habitats characterized by different degrees of anthropogenic disturbance...
- Melanelixia and Melanohalea, two new genera segregated from Melanelia (Parmeliaceae) based on molecular and morphological dataOscar Blanco
Departamento de Biologia Vegetal II, Facutad de Farmacia, Universidad Complutense, Plaza de Ramon y Cajal, Ciudad Universitaria, Madrid 28040, Spain
Mycol Res 108:873-84. 2004..disjuncta and its related species remains uncertain, linking with the Xanthoparmelia (syn. Neofuscelia) clade but also without strong support...
- Phylogeny and ecological radiation of New World thistles (Cirsium, Cardueae - Compositae) based on ITS and ETS rDNA sequence dataDean G Kelch
Department of Integrative Biology, University of California, Berkeley, CA 94720, USA
Mol Ecol 12:141-51. 2003..These results indicate either that Cirsium underwent a rapid ecological radiation in North America, or that rDNA evolution in North American Cirsium has been highly conservative...
- Secondary structure models of the nuclear internal transcribed spacer regions and 5.8S rRNA in Calciodinelloideae (Peridiniaceae) and other dinoflagellatesMarc Gottschling
Freie Universitat Berlin, Institut für Geologische Wissenschaften Fachrichtung Paläontologie, Malteserstrasse 74 100, D 12249 Berlin, Germany
Nucleic Acids Res 32:307-15. 2004..Thus, the structure models developed in this study may be generally useful for future phylogenetic analyses...
- The mosaic nature of intergenic 16S-23S rRNA spacer regions suggests rRNA operon copy number variation in Clostridium difficile strainsNourkhoda Sadeghifard
Biotechnology Research Centre, La Trobe University, Bendigo, Victoria 3552, Australia
Appl Environ Microbiol 72:7311-23. 2006..difficile...
- Reviewing lymnaeid vectors of fascioliasis by ribosomal DNA sequence analysesM D Bargues
Departamento de Parasitologia, Facultad de Farmacia, Universidad de Valencia, Av Vicente Andrés Estellés s n, 46100 Burjassot, Valencia, Spain
J Helminthol 79:257-67. 2005....
- Molecular identification and phylogeny of the Maculatus group of Anopheles mosquitoes (Diptera: Culicidae) based on nuclear and mitochondrial DNA sequencesYajun Ma
Department of Etiologic Biology, Second Military Medical University, Shanghai 200433, China
Acta Trop 99:272-80. 2006..Our data concludes that An. dispar and An. greeni belong to the Maculatus subgroup, and An. willmori is not closer to either of the subgroups...
- Structure, molecular evolution, and phylogenetic utility of the 5(') region of the external transcribed spacer of 18S-26S rDNA in Lessingia (Compositae, Astereae)Staci Markos
Jepson Herbarium and Department of Integrative Biology, University of California, Berkeley, CA 94720 2465, USA
Mol Phylogenet Evol 23:214-28. 2002..We conclude that challenges in assessing subrepeat orthology across taxa greatly limit the utility of the 5(')ETS region for phylogenetic analyses among species of Lessingia...
- Polymorphism of the ITS-2 region of the ribosomal DNA of the Triatominae Rhodnius domesticus, R. pictipes, R. prolixus and R. staliM P Naegele
Departamento de Ciencias Biologicas, Faculdade de Ciênciasa, Farmacêutivas, UNESP, Araraquara, Sao Paulo, Brazil
Med Vet Entomol 20:353-7. 2006..domesticus, R. pictipes and R. prolixus, and HhaI differentiated R. domesticus, R. pictipes and R. stali. With the fingerprinting analysis generated by these two enzymes, it was possible to clearly identify all four species in the study...
- Hyperdiversity of ectomycorrhizal fungus assemblages on oak seedlings in mixed forests in the southern Appalachian MountainsJohn F Walker
Department of Biology, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24060, USA
Mol Ecol 14:829-38. 2005..The high mycobiont diversity highlights the potential for seedlings to acquire carbon from mycelial networks and confirms the utility of using outplanted seedlings to estimate ectomycorrhizal diversity...
- Only a few fungal species dominate highly diverse mycofloras associated with the common reedKarin Neubert
Lehrstuhl Phytopathologie, Fachbereich Biologie, Universitat Konstanz, Universitätsstr 10, D 78457 Konstanz, Germany
Appl Environ Microbiol 72:1118-28. 2006..This suggestion was independently supported by the results of nested PCR assays specifically monitoring two OTUs over 3 years, which revealed significant preferences for host habitat and host organ...
- Internal transcribed spacers (ITS) of Trypanosoma rangeli ribosomal DNA (rDNA): a useful marker for inter-specific differentiationI T Beltrame-Botelho
Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Catarina, Caixa Postal 476, 88040 900, Florianopolis, SC, Brazil
Infect Genet Evol 5:17-28. 2005..Sequence analysis of both ITS spacers plus the 5.8S rDNA of T. rangeli strains allowed a clear inter-specific differentiation from Trypanosoma cruzi strains representative of the parasite zymodemes...
- Molecular epidemiology of Scedosporium apiospermum infection determined by PCR amplification of ribosomal intergenic spacer sequences in patients with chronic lung diseaseE C Williamson
Division of Microbiology and Infectious Diseases, The Western Australian Center for Pathology and Medical Research PathCentre, Nedlands 6009, Australia
J Clin Microbiol 39:47-50. 2001..There was no epidemiological evidence of direct patient-to-patient spread, and Scedosporium organisms were not isolated from dust, soil, or air samples from the unit. The source and route of transmission have yet to be determined...
- ITS2 sequences as barcodes for identifying and analyzing spider mites (Acari: Tetranychidae)Tselila Ben-David
Department of Entomology, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot 76100, Israel
Exp Appl Acarol 41:169-81. 2007..We predict that our work, together with similar efforts, will provide in the future the platform for a uniform, accurate, practical and easy-to-use method of spider mite species identification...
- The ITS2 Database II: homology modelling RNA structure for molecular systematicsChristian Selig
Department of Bioinformatics, Biocenter, University of Wurzburg, Am Hubland 97074 Würzburg, Germany
Nucleic Acids Res 36:D377-80. 2008..These genera might have evolved an ITS2 structure diverging from the standard one. The current version of the ITS2 Database can be accessed via http://its2.bioapps.biozentrum.uni-wuerzburg.de...
- Biogeographical and phylogenetic origins of African fig species (Ficus section Galoglychia)Nina Rønsted
Jodrell Laboratory, Royal Botanic Gardens, Kew, Richmond, Surrey TW9 3DS, UK
Mol Phylogenet Evol 43:190-201. 2007..The other main clade includes members of subsections Caulocarpae, Cyathistipulae, Crassicostae and Galoglychia, which are concentrated in West and Central Africa...
- Molecular characterization of nontuberculous mycobacteria isolated from human cases of disseminated disease in the USA, Thailand, Malawi, and TanzaniaJason Ballard
National Reference Centre for Mycobacteriology, National Microbiology Laboratory, Health Canada, Winnipeg, Manitoba, Canada
J Gen Appl Microbiol 53:153-7. 2007
- Mycobacterium sherrisii isolation from a patient with pulmonary diseaseEnrico Tortoli
Regional Reference Center for Mycobacteria, Careggi Hospital, 50134 Florence, Italy
Diagn Microbiol Infect Dis 57:221-3. 2007..One of our strains was responsible for pulmonary disease in a middle-aged non-HIV patient; thus, confirming the potential pathogenicity of this species previously reported only in an HIV-positive patient...
- Genetically closely related but phenotypically divergent Trichoderma species cause green mold disease in oyster mushroom farms worldwideMonika Komon-Zelazowska
Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Getreidemarkt 9 1665, A 1060 Vienna, Austria
Appl Environ Microbiol 73:7415-26. 2007..A DNA BarCode for identification of these species based on ITS1 and ITS2 sequences has been provided and integrated in the main database for Hypocrea/Trichoderma (www.ISTH.info)...
- 5.8S-28S rRNA interaction and HMM-based ITS2 annotationAlexander Keller
Department of Bioinformatics, University of Wurzburg, Biocenter, Am Hubland, 97074 Wurzburg, Germany
Gene 430:50-7. 2009..Sequences can be submitted to the following website for HMM-based ITS2 delineation: http://its2.bioapps.biozentrum.uni-wuerzburg.de...
- Characterization of Cryptocaryon irritans isolates from marine fishes in Mainland China by ITS ribosomal DNA sequencesH Y Sun
School of Life Sciences, State Key Laboratory of Biocontrol Sun Yat sen University, 135 Xingang West Street, Haizhu District, Guangzhou 510275, Guangdong Province, People s Republic of China
Parasitol Res 99:160-6. 2006..irritans from Mainland China and elsewhere and is also relevant to the diagnosis and control of fish diseases they cause...
- Rapid identification of Trichophyton tonsurans by specific PCR based on DNA sequences of nuclear ribosomal internal transcribed spacer (ITS) 1 regionEri Yoshida
Department of Dermatology, Tokyo Medical University, Nishishinjuku, 6 1 1 Shinjuku ku, and Department of Dermatology, Fraternity Memorial Hospital, Tokyo 160 0023, Japan
J Dermatol Sci 42:225-30. 2006..Trichophyton tonsurans, a dermatophyte implicated in an international epidemic of tinea capitis, was also found to be responsible for infecting wrestling and Judo athletes nationwide in Japan since 2001...
- Molecular identification of zygomycetes from culture and experimentally infected tissuesPatrick Schwarz
Centre National de Référence Mycologie et Antifongiques, Unité de Mycologie Moléculaire, CNRS FRE2849, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France
J Clin Microbiol 44:340-9. 2006....
- Recent oceanic long-distance dispersal and divergence in the amphi-Atlantic rain forest genus Renealmia L.f. (Zingiberaceae)Tiina E Särkinen
Royal Botanic Garden Edinburgh, 20a Inverleith Row, EH3 5LR Edinburgh, UK
Mol Phylogenet Evol 44:968-80. 2007..Speciation has been approximately simultaneous on both sides of the Atlantic, but increased taxon sampling is required to compare the speciation rates between the New World and Old World tropics...
- Molecular phylogeny of the Pseudallescheria boydii species complex: proposal of two new speciesFelix Gilgado
Unitat de Microbiologia Facultat de Medicina i Ciencias de la Salut, Universitat Rovira i Virgili C Sant Llorenç 21, 43201 Reus, Tarragona, Spain
J Clin Microbiol 43:4930-42. 2005..minutispora are environmental. Further studies are needed to demonstrate whether all the species included in the P. boydii complex have different clinical spectra and antifungal susceptibility...
- Evolution and alignment of the hypervariable arm 1 of Aeschynanthus (Gesneriaceae) ITS2 nuclear ribosomal DNAJ Denduangboripant
Institute of Cell and Molecular Biology, The University of Edinburgh, Edinburgh, EH9 3JH, United Kingdom
Mol Phylogenet Evol 20:163-72. 2001..Alignment based on secondary structure conflicts with our initial manual alignment and, to a lesser extent, with a CLUSTAL X alignment with default parameters...
- Nonuniform concerted evolution and chloroplast capture: heterogeneity of observed introgression patterns in three molecular data partition phylogenies of Asian Mitella (saxifragaceae)Yudai Okuyama
Graduate School of Human and Environmental Studies, Kyoto University, Kyoto, Japan
Mol Biol Evol 22:285-96. 2005....
- Postgenomic taxonomy of human ureaplasmas -- a case study based on multiple gene sequencesFanrong Kong
Centre for Infectious Diseases and Microbiology CIDM, Institute of Clinical Pathology and Medical Research ICPMR, Westmead, New South Wales, Australia
Int J Syst Evol Microbiol 54:1815-21. 2004..It is recommended that, to avoid confusion, the new nomenclature be used for human ureaplasmas in future publications...
- Non-homogenized ITS regions in the parasitic nematode Cooperia oncophoraT H M Mes
Department of Parasitology and Tropical Veterinary Medicine, Institute of Infectious Diseases and Immunology, Utrecht University, 3508 TD Utrecht, The Netherlands
Parasitology 129:213-22. 2004..Finally, although it can not be rigorously assessed in Cooperia, the ITS polymorphisms can readily be envisioned to affect phylogenetic reconstructions of closely related nematodes...
- Lower level relationships in the mushroom genus Cortinarius (Basidiomycota, Agaricales): a comparison of RPB1, RPB2, and ITS phylogeniesT G Frøslev
Department of Microbiology, Institute of Biology, University of Copenhagen, Øster Farimagsgade 2D, DK 1353 Copenhagen K, Denmark
Mol Phylogenet Evol 37:602-18. 2005..We speculate that sequence information from RNA polymerase II genes have the potential for resolving phylogenetic problems at several levels of the diverse and taxonomically very challenging genus Cortinarius...
- Mycobionts of Salix herbacea on a glacier forefront in the Austrian AlpsOliver Mühlmann
Institute of Microbiology, University Innsbruck, Technikerstr 25, 6020 Innsbruck, Austria
Mycorrhiza 18:171-80. 2008..This suggests that the mycorrhizae were displaced from the roots by an outcompeting species, whereas the mycelium was still present in the soil...
- Intragenomic variation in the internal transcribed spacer 1 region of Dientamoeba fragilis as a molecular epidemiological markerAldert Bart
Section Parasitology, Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, Meibergdreef 9, Amsterdam 1105 AZ, The Netherlands
J Clin Microbiol 46:3270-5. 2008..fragilis directly from feces. This may serve as a means of studying the transmission, geographical distribution, and relationships between strains and the pathogenicity of this parasite...
- The allopolyploid Arabidopsis kamchatica originated from multiple individuals of Arabidopsis lyrata and Arabidopsis halleriRie Shimizu-Inatsugi
Institute of Plant Biology, University of Zurich, Zurich CH 8008, Switzerland
Mol Ecol 18:4024-48. 2009..The haplotypes of low-copy nuclear genes in Japan suggest independent polyploidization events rather than introgression. Our findings suggest that self-compatibility and gene silencing occurred independently in different origins...
- Shape-shifting corals: molecular markers show morphology is evolutionarily plastic in PoritesZac H Forsman
Hawaii Institute of Marine Biology, Kaneohe, HI 96744, USA
BMC Evol Biol 9:45. 2009....
- Polymerase chain reaction-based identification and genotyping of Anopheles mosquitoes with a 96-pin bacterial replicatorCristina S Rafferty
Centers for Disease Control and Prevention, Division of Parasitic Diseases, Atlanta, Georgia, USA
Am J Trop Med Hyg 66:234-7. 2002..This method allows rapid analysis of large numbers of mosquitoes without robotic equipment and should enable rapid and extensive PCR analysis of field-collected samples and laboratory specimens...
- Three nonorthologous ITS1 types are present in a polypore fungus Trichaptum abietinumKwan Soo Ko
School of Biological Sciences, Seoul National University, San 56 1 Shinrim Dong, Kwanag gu, Seoul 151 742, Republic of Korea
Mol Phylogenet Evol 23:112-22. 2002..However, Type III was amplified only in three T. abietinum strains collected from Korea, indicating that a new local geographic subtype has arisen in Korean strains...
- Ribosomal DNA spacer genotypes of the Anopheles bancroftii group (Diptera: Culicidae) from Australia and Papua New GuineaN W Beebe
Department of Cell and Molecular Biology, University of Technology Sydney, Australia
Insect Mol Biol 10:407-13. 2001..Genotypes B and C and genotypes C and D were found sympatric and appeared to be evolving independently suggesting the possibility of cryptic species. Genotype C contained two ITS2 sequence types within the genome...
- Molecular phylogeny of hybridizing species from the genus Spartina Schreb. (Poaceae)A Baumel
UMR, CNRS, 6553 Université de Rennes1, Evolution des Populations et des Espèces, Rennes, France
Mol Phylogenet Evol 22:303-14. 2002..alterniflora and S. foliosa) on one hand, and alloploid speciation involving more differentiated species (S. alterniflora and S. maritima) on the other hand...
- Intraspecific and within-isolate sequence variation in the ITS rRNA gene region of Pythium mercuriale sp. nov. (Pythiaceae)Lassaad Belbahri
Plants and Pathogens Group, Research Institute Earth Nature and Landscape, School of Engineering of Lullier, University of Applied Sciences of Western Switzerland, Jussy, Switzerland
FEMS Microbiol Lett 284:17-27. 2008..Maximum likelihood phylogenetic analyses based on both ITS and beta-tubulin sequence data place P. mercuriale in a clade between Pythium and Phytophthora...
- Native range genetic variation in Arabidopsis thaliana is strongly geographically structured and reflects Pleistocene glacial dynamicsJames B Beck
Department of Biology, Washington University, St Louis, MO 63130, USA
Mol Ecol 17:902-15. 2008..Spatial autocorrelation analyses indicate that isolation by distance is pervasive at all hierarchical levels, but that it is reduced in portions of Europe...
- Organization, differential expression and methylation of rDNA in artificial Solanum allopolyploidsNataliya Y Komarova
Department of General Genetics, Center of Plant Molecular Biology ZMBP, University of Tubingen, Auf der Morgenstelle 28, Tubingen, 72076, Germany
Plant Mol Biol 56:439-63. 2004....
- Unusual structure of ribosomal DNA in the copepod Tigriopus californicus: intergenic spacer sequences lack internal subrepeatsR S Burton
Marine Biology Research Division 0202, Scripps Institution of Oceanography, University of California, San Diego, La Jolla, CA 92093 0202, USA
Gene 344:105-13. 2005....
- FUNGAL PROTEIN/GLYCOPROTEIN EXPRESSION IN TINEA CAPITISSusan Abdel Rahman; Fiscal Year: 2003..abstract_text> ..
- Evaluating Trichophyton tonsurans Carriage and InfectionSusan Abdel Rahman; Fiscal Year: 2006..abstract_text> ..
- High through-put detection of fungal pathogensJACK FELL; Fiscal Year: 2004..Resulting data will be disseminated via GenBank, publications in leading scientific journals and as an inexpensive commercial detection system. ..
- Genetic Transformation of Cryptosporidium parvumGiovanni Widmer; Fiscal Year: 2005..Approach: Transformation by electroporation of sporozoites with GFP expression plasmids; detection of transformants in cell culture by fluorescent microscopy, anti-GFP antibodies, or reverse-transcriptase PCR. ..
- Genetics of Cryptosporidium parvumGiovanni Widmer; Fiscal Year: 2008..Mutations will be mapped to the genome of C. hominis, C. muris and more distantly related apicomplexan to identify those most likely to affect protein function or transcriptional regulation. ..
- Microbial Correlates of Bacterial Vaginosis Treatment Failure and Recurrence in HMichael Ferris; Fiscal Year: 2008..Significance: The results should lead to more effective methods of diagnosing and treating BV, and preventing the serious sequelae associated with this common syndrome. ..
- LARVAL MOSQUITO FEEDING AND MICROBIAL INTERACTIONSEdward Walker; Fiscal Year: 2007..abstract_text> ..
- Topical Antibiotics to Prevent Lyme DiseaseGary Wormser; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- Functional Genomics of Cryptosporidium parvumGiovanni Widmer; Fiscal Year: 2007..parvum. A comparison of the host cell response to C. parvum type 1 and type 2 will be performed to probe the molecular basis of the observed difference in virulence between types. ..
- Population genetic structure and bionomics of Anopheles lesteri in ChinaYajun Ma; Fiscal Year: 2007..lesteri mosquitoes as well as the geographic variation of its bionomics. This information is particularly useful for the design of rational area-specific vector control methods. [unreadable] [unreadable]..
- Insecticide mosaics and sustainability of treated netsEdward Walker; Fiscal Year: 2007..Industry partners Vestergaard-Frandsen and Syngenta are directly involved in these efforts, for formulation and evaluation purposes. ..
- Mark I Model 68A Rodent/Cell line Gamma IrradiatorSanford Feldman; Fiscal Year: 2004....
- A Genome Sequence Survey of Enterocytozoon bieneusiDonna Akiyoshi; Fiscal Year: 2006..c. Establishment of a website to provide access to the sequence data and clones by the scientific community. ..
- LARVAL MOSQUITO FEEDING AND MICROBIAL INTERACTIONSEdward Walker; Fiscal Year: 2006..3) Studies of transformant efficacy against mosquito larvae, and persistence in simulated mosquito habitats. ..
- Role of non-tuberculous mycobacteria in lung diseaseJason Stout; Fiscal Year: 2006..Our findings will also assist clinicians in predicting which patients with NTM isolated from the respiratory tract will have NTM pulmonary disease. ..
- Microbial Mediation of Habitat Selection and ProductionEdward Walker; Fiscal Year: 2006..Microbial basis for larval habitat production will be studied with field and lab experiments where nutrient sources and microbial communities are quantified, manipulated and larval growth responses and adult production quantified. ..
- Genetics of Cryptosporidium parvumGiovanni Widmer; Fiscal Year: 2010..Such valleys will be identified by quantitatively genotyping a selected and a control progeny population with a panel of single nucleotide polymorphisms. ..