dna transposable elements

Summary

Summary: Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.

Top Publications

  1. ncbi The B73 maize genome: complexity, diversity, and dynamics
    Patrick S Schnable
    Center for Plant Genomics, Iowa State University, Ames, IA 50011, USA
    Science 326:1112-5. 2009
  2. ncbi Repbase Update, a database of eukaryotic repetitive elements
    J Jurka
    Genetic Information Research Institute, Mountain View, CA 94043, USA
    Cytogenet Genome Res 110:462-7. 2005
  3. ncbi Initial sequencing and analysis of the human genome
    E S Lander
    Whitehead Institute for Biomedical Research, Center for Genome Research, Cambridge, Massachusetts 02142, USA
    Nature 409:860-921. 2001
  4. ncbi A unified classification system for eukaryotic transposable elements
    Thomas Wicker
    Institute of Plant Biology, University Zurich, Zollikerstrasse 107, Ch 8008 Zurich, Switzerland
    Nat Rev Genet 8:973-82. 2007
  5. ncbi The complete genome sequence of Escherichia coli K-12
    F R Blattner
    Laboratory of Genetics, University of Wisconsin Madison, 445 Henry Mall, Madison, WI 53706, USA
    Science 277:1453-62. 1997
  6. pmc Genome sequence of Aedes aegypti, a major arbovirus vector
    Vishvanath Nene
    Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Science 316:1718-23. 2007
  7. ncbi Genes required for mycobacterial growth defined by high density mutagenesis
    Christopher M Sassetti
    Department of Immunology and Infectious Diseases, Harvard School of Public Health, Harvard Medical School, Boston, MA 02115, USA
    Mol Microbiol 48:77-84. 2003
  8. pmc The TIGR Rice Genome Annotation Resource: improvements and new features
    Shu Ouyang
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Nucleic Acids Res 35:D883-7. 2007
  9. ncbi Discrete small RNA-generating loci as master regulators of transposon activity in Drosophila
    Julius Brennecke
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences and Howard Hughes Medical Institute, Cold Spring Harbor, NY 11724, USA
    Cell 128:1089-103. 2007
  10. pmc Repetitive elements may comprise over two-thirds of the human genome
    A P Jason De Koning
    Department of Biochemistry and Molecular Genetics, School of Medicine, University of Colorado, Aurora, Colorado, USA
    PLoS Genet 7:e1002384. 2011

Research Grants

  1. Selenium, Aminothiols, and Radiation
    Alan Diamond; Fiscal Year: 2004
  2. Genetic Manipulation of Insects
    DAVID O BROCHTA; Fiscal Year: 2004
  3. HOBO-LIKE ELEMENTS IN INSECTS
    DAVID O BROCHTA; Fiscal Year: 2008
  4. Tn5 as an insect genomics tool
    DAVID O BROCHTA; Fiscal Year: 2002
  5. MECHANISMS OF MYCOPLASMA-INDUCED ARTHRITIS
    Kevin Dybvig; Fiscal Year: 2009
  6. Tandemly Repetitive Proteins in Mycoplasmas
    KEVIN F DYBVIG; Fiscal Year: 2010
  7. YEAST GENETIC INTERACTION MAP
    JEF BOEKE; Fiscal Year: 2008
  8. Genetic methods using the Tol2 transposable element
    Koichi Kawakami; Fiscal Year: 2006
  9. TRANSCRIPTIONAL SILENCING MECHANISMS
    JEF BOEKE; Fiscal Year: 2005
  10. Identification of Tetrahymena DNA Rearrangement Genes
    DOUGLAS LEE CHALKER; Fiscal Year: 2010

Detail Information

Publications276 found, 100 shown here

  1. ncbi The B73 maize genome: complexity, diversity, and dynamics
    Patrick S Schnable
    Center for Plant Genomics, Iowa State University, Ames, IA 50011, USA
    Science 326:1112-5. 2009
    ..These analyses inform and set the stage for further investigations to improve our understanding of the domestication and agricultural improvements of maize...
  2. ncbi Repbase Update, a database of eukaryotic repetitive elements
    J Jurka
    Genetic Information Research Institute, Mountain View, CA 94043, USA
    Cytogenet Genome Res 110:462-7. 2005
    ..Sequences from Repbase Update are used to screen and annotate repetitive elements using programs such as Censor and RepeatMasker. Repbase Update is available on the worldwide web at http://www.girinst.org/Repbase_Update.html...
  3. ncbi Initial sequencing and analysis of the human genome
    E S Lander
    Whitehead Institute for Biomedical Research, Center for Genome Research, Cambridge, Massachusetts 02142, USA
    Nature 409:860-921. 2001
    ..We also present an initial analysis of the data, describing some of the insights that can be gleaned from the sequence...
  4. ncbi A unified classification system for eukaryotic transposable elements
    Thomas Wicker
    Institute of Plant Biology, University Zurich, Zollikerstrasse 107, Ch 8008 Zurich, Switzerland
    Nat Rev Genet 8:973-82. 2007
    ..The system and nomenclature is kept up to date at the WikiPoson web site...
  5. ncbi The complete genome sequence of Escherichia coli K-12
    F R Blattner
    Laboratory of Genetics, University of Wisconsin Madison, 445 Henry Mall, Madison, WI 53706, USA
    Science 277:1453-62. 1997
    ..The genome also contains insertion sequence (IS) elements, phage remnants, and many other patches of unusual composition indicating genome plasticity through horizontal transfer...
  6. pmc Genome sequence of Aedes aegypti, a major arbovirus vector
    Vishvanath Nene
    Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Science 316:1718-23. 2007
    ..An increase in genes encoding odorant binding, cytochrome P450, and cuticle domains relative to An. gambiae suggests that members of these protein families underpin some of the biological differences between the two mosquito species...
  7. ncbi Genes required for mycobacterial growth defined by high density mutagenesis
    Christopher M Sassetti
    Department of Immunology and Infectious Diseases, Harvard School of Public Health, Harvard Medical School, Boston, MA 02115, USA
    Mol Microbiol 48:77-84. 2003
    ....
  8. pmc The TIGR Rice Genome Annotation Resource: improvements and new features
    Shu Ouyang
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Nucleic Acids Res 35:D883-7. 2007
    ..Enhanced data access is available through web interfaces, FTP downloads and a Data Extractor tool developed in order to support discrete dataset downloads...
  9. ncbi Discrete small RNA-generating loci as master regulators of transposon activity in Drosophila
    Julius Brennecke
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences and Howard Hughes Medical Institute, Cold Spring Harbor, NY 11724, USA
    Cell 128:1089-103. 2007
    ..Thus, sense piRNAs, formed following cleavage of transposon mRNAs may enhance production of antisense piRNAs, complementary to active elements, by directing cleavage of transcripts from master control loci...
  10. pmc Repetitive elements may comprise over two-thirds of the human genome
    A P Jason De Koning
    Department of Biochemistry and Molecular Genetics, School of Medicine, University of Colorado, Aurora, Colorado, USA
    PLoS Genet 7:e1002384. 2011
    ..These results highlight the need for combined, probabilistic genome annotation approaches and suggest that the human genome consists of substantially more repetitive sequence than previously believed...
  11. ncbi Transposable elements and the epigenetic regulation of the genome
    R Keith Slotkin
    Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nat Rev Genet 8:272-85. 2007
    ..In addition, new insights have been gained into how this silencing has been co-opted to serve essential functions in 'host' cells, highlighting the importance of TEs in the epigenetic regulation of the genome...
  12. pmc DNA transposons and the evolution of eukaryotic genomes
    CEDRIC FESCHOTTE
    Department of Biology, University of Texas, Arlington, TX 76019, USA
    Annu Rev Genet 41:331-68. 2007
    ..Finally, we highlight how the distinctive biological features of DNA transposons have contributed to shape genome architecture and led to the emergence of genetic innovations in different eukaryotic lineages...
  13. pmc Annotation, submission and screening of repetitive elements in Repbase: RepbaseSubmitter and Censor
    Oleksiy Kohany
    Genetic Information Research Institute, 1925 Landings Drive, Mountain View, CA 94043, USA
    BMC Bioinformatics 7:474. 2006
    ..Updating and maintenance of the database requires specialized tools, which we have created and made available for use with Repbase, and which may be useful as a template for other curated databases...
  14. ncbi Mobile elements: drivers of genome evolution
    Haig H Kazazian
    Department of Genetics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Science 303:1626-32. 2004
    ..Although the host can often control their numbers, massive expansions of retrotransposons have been tolerated during evolution. Now mobile elements are becoming useful tools for learning more about genome evolution and gene function...
  15. ncbi Horizontal gene transfer in eukaryotic evolution
    Patrick J Keeling
    Department of Botany, University of British Columbia, Vancouver, British Columbia V6T 1Z, Canada
    Nat Rev Genet 9:605-18. 2008
    ..Major recent trends include the important role of HGT in adaptation to certain specialized niches and the highly variable impact of HGT in different lineages...
  16. pmc ISfinder: the reference centre for bacterial insertion sequences
    P Siguier
    Laboratoire de Microbiologie et Genetique Moleculaires, C N R S 118, route de Narbonne, F 31062 Toulouse Cedex, France
    Nucleic Acids Res 34:D32-6. 2006
    ..Finally, this database is currently recommended by several microbiology journals for registration of new IS elements before their publication...
  17. pmc An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutants
    Nicole T Liberati
    Department of Genetics, Harvard Medical School, Boston, MA 02114, USA
    Proc Natl Acad Sci U S A 103:2833-8. 2006
    ..Comparison of the genes disrupted in the PA14 transposon insertion library with an independently constructed insertion library in P. aeruginosa strain PAO1 provides an estimate of the number of P. aeruginosa essential genes...
  18. ncbi Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells
    Z Ivics
    Department of Genetics and Cell Biology, University of Minnesota, St Paul 55108 1095, USA
    Cell 91:501-10. 1997
    ..Sleeping Beauty is an active DNA-transposon system from vertebrates for genetic transformation and insertional mutagenesis...
  19. pmc The essential genome of a bacterium
    Beat Christen
    Department of Developmental Biology, Stanford University, Stanford, CA, USA
    Mol Syst Biol 7:528. 2011
    ..The high-resolution strategy used here is applicable to high-throughput, full genome essentiality studies and large-scale genetic perturbation experiments in a broad class of bacterial species...
  20. ncbi Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice
    Sheng Ding
    Institute of Developmental Biology and Molecular Medicine, School of Life Sciences, Fudan University, 220 Handan Road, Shanghai 200433, China
    Cell 122:473-83. 2005
    ..These data provide a first and critical step toward a highly efficient transposon system for a variety of genetic manipulations including transgenesis and insertional mutagenesis in mice and other vertebrates...
  21. ncbi PIWI-interacting small RNAs: the vanguard of genome defence
    Mikiko C Siomi
    Keio University School of Medicine, Tokyo 160 8582, Japan
    Nat Rev Mol Cell Biol 12:246-58. 2011
    ..Now, much progress is being made into our understanding of their biogenesis and molecular functions, including the specific subcellular compartmentalization of the piRNA pathway in granular cytoplasmic bodies...
  22. ncbi The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome
    Mohammed Sebaihia
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK
    Nat Genet 38:779-86. 2006
    ..The extreme genome variability was confirmed by whole-genome microarray analysis; it may reflect the organism's niche in the gut and should provide information on the evolution of virulence in this organism...
  23. pmc Transposable elements and the evolution of regulatory networks
    CEDRIC FESCHOTTE
    Department of Biology, Life Science Building, Box 19498, University of Texas, Arlington, Texas 76019, USA
    Nat Rev Genet 9:397-405. 2008
    ..Here I revisit and expand earlier models and propose that genomic repeats, and in particular transposable elements, have been a rich source of material for the assembly and tinkering of eukaryotic gene regulatory systems...
  24. ncbi Transposable elements have rewired the core regulatory network of human embryonic stem cells
    Galih Kunarso
    Computational and Mathematical Biology, Genome Institute of Singapore, Singapore, Singapore
    Nat Genet 42:631-4. 2010
    ..These data indicate that species-specific transposable elements have substantially altered the transcriptional circuitry of pluripotent stem cells...
  25. ncbi Genome sequence and analysis of the Irish potato famine pathogen Phytophthora infestans
    Brian J Haas
    Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02141, USA
    Nature 461:393-8. 2009
    ..infestans genome. This probably plays a crucial part in the rapid adaptability of the pathogen to host plants and underpins its evolutionary potential...
  26. ncbi Green evolution and dynamic adaptations revealed by genomes of the marine picoeukaryotes Micromonas
    Alexandra Z Worden
    Monterey Bay Aquarium Research Institute, Moss Landing, CA 95039 USA
    Science 324:268-72. 2009
    ..Analyses of the Micromonas genomes offer valuable insights into ecological differentiation and the dynamic nature of early plant evolution...
  27. ncbi Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable gene transfer in vertebrates
    Lajos Mátés
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Nat Genet 41:753-61. 2009
    ....
  28. ncbi Evolution of genes and genomes on the Drosophila phylogeny
    Andrew G Clark
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
    Nature 450:203-18. 2007
    ..These may prove to underlie differences in the ecology and behaviour of these diverse species...
  29. pmc Epigenetic reprogramming and small RNA silencing of transposable elements in pollen
    R Keith Slotkin
    Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Cell 136:461-72. 2009
    ..We propose a conserved role for reprogramming in germline companion cells, such as nurse cells in insects and vegetative nuclei in plants, to reveal intact TEs in the genome and regulate their activity in gametes...
  30. ncbi A transposon-mediated gene trap approach identifies developmentally regulated genes in zebrafish
    Koichi Kawakami
    Division of Molecular and Developmental Biology, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411 8540, Japan
    Dev Cell 7:133-44. 2004
    ..We propose our gene trap approach should facilitate studies of vertebrate development and organogenesis...
  31. pmc Simultaneous assay of every Salmonella Typhi gene using one million transposon mutants
    Gemma C Langridge
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, United Kingdom
    Genome Res 19:2308-16. 2009
    ..Typhi in the gall bladder. This screen validated our hypothesis that we can simultaneously assay every gene in the genome to identify niche-specific essential genes...
  32. pmc Miniature inverted-repeat transposable elements (MITEs) have been accumulated through amplification bursts and play important roles in gene expression and species diversity in Oryza sativa
    Chen Lu
    Key Laboratory of Horticulture Biology, Ministry of Education and Department of Vegetable Crops, College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan, People s Republic of China
    Mol Biol Evol 29:1005-17. 2012
    ..sativa ssp. indica) and Nipponbare. Considering that different sets of genes may be regulated by MITE-derived small RNAs in different genotypes, MITEs provide considerable diversity for O. sativa...
  33. ncbi P[acman]: a BAC transgenic platform for targeted insertion of large DNA fragments in D. melanogaster
    Koen J T Venken
    Program in Developmental Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Science 314:1747-51. 2006
    ..This transgenesis platform should greatly facilitate structure/function analyses of most Drosophila genes...
  34. pmc A piRNA pathway primed by individual transposons is linked to de novo DNA methylation in mice
    Alexei A Aravin
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Mol Cell 31:785-99. 2008
    ..This implicates piRNAs as specificity determinants of DNA methylation in germ cells...
  35. ncbi Transposon-mediated rewiring of gene regulatory networks contributed to the evolution of pregnancy in mammals
    Vincent J Lynch
    Department of Ecology and Evolutionary Biology and Yale Systems Biology Institute, Yale University, New Haven, Connecticut, USA
    Nat Genet 43:1154-9. 2011
    ....
  36. pmc Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon
    Kosuke Yusa
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
    Nat Methods 6:363-9. 2009
    ..iPSCs fulfilled all criteria of pluripotency, such as pluripotency gene expression, teratoma formation and contribution to chimeras. piggyBac transposon-based reprogramming may be used to generate therapeutically applicable iPSCs...
  37. pmc Plasticity of animal genome architecture unmasked by rapid evolution of a pelagic tunicate
    France Denoeud
    Commissariat a l Energie Atomique, Institut de Génomique, Genoscope, Evry, France
    Science 330:1381-5. 2010
    ..This rapidly evolving animal lineage thus offers unique perspectives on the level of genome plasticity. It also illuminates issues as fundamental as the mechanisms of intron gain...
  38. pmc Tracking insertion mutants within libraries by deep sequencing and a genome-wide screen for Haemophilus genes required in the lung
    Jeffrey D Gawronski
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, 55 Lake Avenue North, S6 242, Worcester, MA 01655, USA
    Proc Natl Acad Sci U S A 106:16422-7. 2009
    ....
  39. ncbi The genome of Theobroma cacao
    Xavier Argout
    Centre de coopération Internationale en Recherche Agronomique pour le Développement CIRAD Biological Systems Department Unité Mixte de Recherche Développement et Amélioration des Plantes UMR DAP TA A 96 03 34398, Montpellier, France
    Nat Genet 43:101-8. 2011
    ..cacao improvement. Based on the inferred paleohistory of the T. cacao genome, we propose an evolutionary scenario whereby the ten T. cacao chromosomes were shaped from an ancestor through eleven chromosome fusions...
  40. ncbi An active DNA transposon family in rice
    Ning Jiang
    Department of Plant Biology, University of Georgia, Athens, Georgia 30602, USA
    Nature 421:163-7. 2003
    ..Since the domestication of rice mPing MITEs have been amplified preferentially in cultivars adapted to environmental extremes-a situation that is reminiscent of the genomic shock theory for transposon activation...
  41. ncbi Tn916-like genetic elements: a diverse group of modular mobile elements conferring antibiotic resistance
    Adam P Roberts
    Department of Microbial Diseases, UCL Eastman Dental Institute, University College London, London, UK
    FEMS Microbiol Rev 35:856-71. 2011
    ..The ability of these conjugative transposons to acquire additional, clinically relevant antibiotic resistance genes importantly contributes to the emergence of multidrug resistance...
  42. pmc Genome of the bacterium Streptococcus pneumoniae strain R6
    J Hoskins
    Infectious Diseases Research and Clinical Investigation, Eli Lilly and Company, Indianapolis, Indiana 46285, USA
    J Bacteriol 183:5709-17. 2001
    ..Among those proteins there may be new targets for vaccine and antibiotic development...
  43. ncbi Origin of primate orphan genes: a comparative genomics approach
    Macarena Toll-Riera
    Evolutionary Genomics Group, Biomedical Informatics Research Programme, Fundació Institut Municipal d Investigació Mèdica, Barcelona, Spain
    Mol Biol Evol 26:603-12. 2009
    ..This indicates frequent recruitment of TEs as part of novel genes. Finally, we also obtain evidence that a small fraction of primate orphan genes, around 5.5%, might have originated de novo from mammalian noncoding genomic regions...
  44. pmc An epigenetic role for maternally inherited piRNAs in transposon silencing
    Julius Brennecke
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory CSHL, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Science 322:1387-92. 2008
    ..This indicates that maternally deposited piRNAs are important for mounting an effective silencing response and that a lack of maternal piRNA inheritance underlies hybrid dysgenesis...
  45. ncbi Unexpected consequences of a sudden and massive transposon amplification on rice gene expression
    Ken Naito
    Department of Plant Biology, University of Georgia, Athens, Georgia 30602, USA
    Nature 461:1130-4. 2009
    ..As such, this study provides evidence for models first proposed previously for the involvement of transposable elements and other repetitive sequences in genome restructuring and gene regulation...
  46. ncbi Repetitive sequences in complex genomes: structure and evolution
    Jerzy Jurka
    Genetic Information Research Institute, Mountain View, California 94043, USA
    Annu Rev Genomics Hum Genet 8:241-59. 2007
    ..Remnants of TEs appear to be overrepresented in transcription regulatory modules and other regions conserved among distantly related species, which may have implications for our understanding of their impact on speciation...
  47. doi A universal classification of eukaryotic transposable elements implemented in Repbase
    Vladimir V Kapitonov
    Nat Rev Genet 9:411-2; author reply 414. 2008
  48. ncbi The composite genome of the legume symbiont Sinorhizobium meliloti
    F Galibert
    UMR6061-CNRS, , , , F-35043 Rennes Cedex, France
    Science 293:668-72. 2001
    ..The genome sequence will be useful in understanding the dynamics of interkingdom associations and of life in soil environments...
  49. ncbi Turning junk into gold: domestication of transposable elements and the creation of new genes in eukaryotes
    Jean Nicolas Volff
    Biofuture Research Group, Physiologische Chemie I, Biozentrum, University of Wurzburg, Am Hubland, D 97074 Wurzburg, Germany
    Bioessays 28:913-22. 2006
    ..Many new candidates for domesticated sequences have been revealed by sequencing projects. Their functional analysis will uncover new aspects of evolutionary alchemy, the turning of junk into gold within genomes...
  50. pmc The struggle for life of the genome's selfish architects
    Aurelie Hua-Van
    Laboratoire Evolution, Génomes, Spéciation, CNRS UPR9034 Université Paris Sud, Gif sur Yvette, France
    Biol Direct 6:19. 2011
    ..We also explore the evolutionary aspects of TEs in the light of post-Darwinian theories of evolution...
  51. pmc Comparative genomic integration profiling of Sleeping Beauty transposons mobilized with high efficacy from integrase-defective lentiviral vectors in primary human cells
    Brian Moldt
    Department of Human Genetics, University of Aarhus, Aarhus, Denmark
    Mol Ther 19:1499-510. 2011
    ..Based on these properties, HIV-1/SB vectors may become valuable tools for genetic engineering and therapeutic gene transfer...
  52. pmc Genetic dissection of neural circuits by Tol2 transposon-mediated Gal4 gene and enhancer trapping in zebrafish
    Kazuhide Asakawa
    Division of Molecular and Developmental Biology, National Institute of Genetics, and Department of Genetics, Graduate University for Advanced Studies, 1111 Yata, Mishima, Shizuoka 411 8540, Japan
    Proc Natl Acad Sci U S A 105:1255-60. 2008
    ..These studies illustrate that our Gal4FF gene trap and enhancer trap methods should be an important resource for genetic analysis of neuronal functions and behavior in vertebrates...
  53. ncbi Transposable elements: powerful facilitators of evolution
    Keith R Oliver
    School of Biological Sciences and Biotechnology, Faculty of Sustainability, Environmental and Life Sciences, Murdoch University, Perth, Australia
    Bioessays 31:703-14. 2009
    ..We propose a general and holistic synthesis on how the presence of TEs within genomes makes them flexible and dynamic, so that genomes themselves are powerful facilitators of their own evolution...
  54. pmc Epigenetic silencing of transposable elements: a trade-off between reduced transposition and deleterious effects on neighboring gene expression
    Jesse D Hollister
    Department of Ecology and Evolutionary Biology, University of Californina, Irvine, Irvine, California 92697 2525, USA
    Genome Res 19:1419-28. 2009
    ..This mechanism implies an evolutionary tradeoff in which the benefit of TE silencing imposes a fitness cost via deleterious effects on the expression of nearby genes...
  55. pmc Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH)
    Roy R Chaudhuri
    Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge, CB3 0ES, UK
    BMC Genomics 10:291. 2009
    ..An important starting point for the development of new antimicrobial drugs is the identification of "essential" genes that are important for bacterial survival and growth...
  56. ncbi A modular master on the move: the Tn916 family of mobile genetic elements
    Adam P Roberts
    Division of Microbial Diseases, UCL Eastman Dental Institute, 256 Gray s Inn Road, London, WC1X 8LD, UK
    Trends Microbiol 17:251-8. 2009
    ..Therefore, the study of this family of mobile genetic elements is essential for a better understanding and control of the current rise of antibiotic resistance among pathogenic bacteria...
  57. ncbi The genome sequence of the malaria mosquito Anopheles gambiae
    Robert A Holt
    Celera Genomics, 45 West Gude Drive, Rockville, MD 20850, USA
    Science 298:129-49. 2002
    ..An expressed sequence tag analysis of genes regulated by blood feeding provided insights into the physiological adaptations of a hematophagous insect...
  58. ncbi Perspective: transposable elements, parasitic DNA, and genome evolution
    M G Kidwell
    Department of Ecology and Evolutionary Biology, The University of Arizona, Tucson 85721, USA
    Evolution 55:1-24. 2001
    ..Of particular interest are transposable element traits that early evolve neutrally at the host level but at a later stage of evolution are co-opted for new host functions...
  59. pmc The catalytic domain of all eukaryotic cut-and-paste transposase superfamilies
    Yao Wu Yuan
    Department of Plant Biology, University of Georgia, Athens, GA 30602, USA
    Proc Natl Acad Sci U S A 108:7884-9. 2011
    Cut-and-paste DNA transposable elements are major components of eukaryotic genomes and are grouped into superfamilies (e.g., hAT, P) based on sequence similarity of the element-encoded transposase...
  60. pmc Endogenous siRNAs derived from transposons and mRNAs in Drosophila somatic cells
    Megha Ghildiyal
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Science 320:1077-81. 2008
    ..We propose that endo-siRNAs generated by the fly RNAi pathway silence selfish genetic elements in the soma, much as Piwi-interacting RNAs do in the germ line...
  61. pmc Human transposon tectonics
    Kathleen H Burns
    Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Cell 149:740-52. 2012
    ..Key questions going forward include how and how much new transposition events affect human health and disease...
  62. pmc piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells
    Knut Woltjen
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada
    Nature 458:766-70. 2009
    ..We anticipate that the unique properties of this virus-independent simplification of iPS cell production will accelerate this field further towards full exploration of the reprogramming process and future cell-based therapies...
  63. ncbi Epigenetic regulation of transposable elements in plants
    Damon Lisch
    Department of Plant and Microbial Biology, University of California, Berkeley, California 94720, USA
    Annu Rev Plant Biol 60:43-66. 2009
    ..Together, these observations suggest that naturally occurring changes in transposon activity may have had an important impact on the causes and consequences of epigenetic silencing in plants...
  64. pmc Transposable elements and small RNAs contribute to gene expression divergence between Arabidopsis thaliana and Arabidopsis lyrata
    Jesse D Hollister
    Department of Ecology and Evolutionary Biology, University of California, Irvine, CA 92697, USA
    Proc Natl Acad Sci U S A 108:2322-7. 2011
    ..Our results suggest that the efficacy of RNA-directed DNA methylation silencing is lower in A. lyrata, a finding that may shed light on the causes of differential TE proliferation among species...
  65. pmc The genome of the Western clawed frog Xenopus tropicalis
    Uffe Hellsten
    Department of Energy Joint Genome Institute, Walnut Creek, CA 94598, USA
    Science 328:633-6. 2010
    ..The genome exhibits substantial shared synteny with human and chicken over major parts of large chromosomes, broken by lineage-specific chromosome fusions and fissions, mainly in the mammalian lineage...
  66. pmc Effector diversification within compartments of the Leptosphaeria maculans genome affected by Repeat-Induced Point mutations
    Thierry Rouxel
    INRA Bioger, UR1290, Avenue Lucien Brétignières, BP 01, Thiverval Grignon F 78850, France
    Nat Commun 2:202. 2011
    ..This genomic environment for effectors promotes rapid sequence diversification and underpins the evolutionary potential of the fungus to adapt rapidly to novel host-derived constraints...
  67. pmc A hyperactive piggyBac transposase for mammalian applications
    Kosuke Yusa
    Wellcome Trust Sanger Institute, Cambridge CB10 1SA, United Kingdom
    Proc Natl Acad Sci U S A 108:1531-6. 2011
    ..This hyperactive piggyBac transposase expands the utility of the piggyBac transposon for applications in mammalian genetics and gene therapy...
  68. pmc Functional dissection of the Tol2 transposable element identified the minimal cis-sequence and a highly repetitive sequence in the subterminal region essential for transposition
    Akihiro Urasaki
    Division of Molecular and Developmental Biology, National Institute of Genetics, Japan
    Genetics 174:639-49. 2006
    ..Also, our study established a basis for further biochemical and molecular biological studies for understanding roles of the repetitive sequence in the subterminal region in transposition...
  69. pmc Next-generation VariationHunter: combinatorial algorithms for transposon insertion discovery
    Fereydoun Hormozdiari
    School of Computing Science, Simon Fraser University, Burnaby, BC, Canada
    Bioinformatics 26:i350-7. 2010
    ....
  70. pmc Promiscuous DNA: horizontal transfer of transposable elements and why it matters for eukaryotic evolution
    Sarah Schaack
    Department of Biology, University of Texas at Arlington, Arlington, TX 76019, USA
    Trends Ecol Evol 25:537-46. 2010
    ..We argue that the introduction of transposable elements by horizontal transfer in eukaryotic genomes has been a major force propelling genomic variation and biological innovation...
  71. pmc A protein trap strategy to detect GFP-tagged proteins expressed from their endogenous loci in Drosophila
    X Morin
    Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609
    Proc Natl Acad Sci U S A 98:15050-5. 2001
    ..GFP reveals in real time the dynamics of protein's distribution in the whole, live organism and provides useful markers for a number of cellular structures and compartments...
  72. pmc Dual coding of siRNAs and miRNAs by plant transposable elements
    Jittima Piriyapongsa
    School of Biology, Georgia Institute of Technology, Atlanta, Georgia 30332 0230, USA
    RNA 14:814-21. 2008
    ..The siRNA-to-miRNA evolutionary transition is representative of a number of other regulatory mechanisms that evolved to silence TEs and were later co-opted to serve as regulators of host gene expression...
  73. pmc Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria
    M Herrero
    GBF National Research Centre for Biotechnology, Braunschweig, Federal Republic of Germany
    J Bacteriol 172:6557-67. 1990
    ..g., in open systems and natural environments), and that do not carry antibiotic resistance markers characteristic of most available cloning vectors (as is currently required of live bacterial vaccines)...
  74. pmc ESSENTIALS: software for rapid analysis of high throughput transposon insertion sequencing data
    Aldert Zomer
    Laboratory of Pediatric Infectious Diseases, Radboud University Medical Centre, Nijmegen, The Netherlands
    PLoS ONE 7:e43012. 2012
    ....
  75. pmc A role for host-parasite interactions in the horizontal transfer of transposons across phyla
    Clément Gilbert
    Department of Biology, University of Texas, Arlington, Texas 76019, USA
    Nature 464:1347-50. 2010
    ..Furthermore, the large amount of DNA generated by the amplification of the horizontally transferred transposons supports the idea that the exchange of genetic material between hosts and parasites influences their genomic evolution...
  76. ncbi Cytosine methylation and the ecology of intragenomic parasites
    J A Yoder
    Department of Genetics and Development, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USA
    Trends Genet 13:335-40. 1997
    ....
  77. pmc DNA transposon Hermes inserts into DNA in nucleosome-free regions in vivo
    Sunil Gangadharan
    Department of Molecular Biology and Genetics, Division of Biostatistics and Bioinformatics, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 107:21966-72. 2010
    ..We have also discovered a long-range sequence motif that defines S. cerevisiae nucleosome-free regions...
  78. pmc The carnegie protein trap library: a versatile tool for Drosophila developmental studies
    Michael Buszczak
    Howard Hughes Medical Institute Research Laboratories, Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland 21218, USA
    Genetics 175:1505-31. 2007
    ....
  79. pmc A transposon-based genetic screen in mice identifies genes altered in colorectal cancer
    Timothy K Starr
    Department of Genetics, Cell Biology and Development, Center for Genome Engineering, Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA
    Science 323:1747-50. 2009
    ..These genes include APC, PTEN, and SMAD4. The screen also identified 17 candidate genes that had not previously been implicated in CRC, including POLI, PTPRK, and RSPO2...
  80. ncbi Massive horizontal gene transfer in bdelloid rotifers
    Eugene A Gladyshev
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Science 320:1210-3. 2008
    ..One such gene, apparently of bacterial origin, was overexpressed in Escherichia coli and yielded an active enzyme. The capture and functional assimilation of exogenous genes may represent an important force in bdelloid evolution...
  81. ncbi Molecular domestication of transposable elements: from detrimental parasites to useful host genes
    L Sinzelle
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Cell Mol Life Sci 66:1073-93. 2009
    ..Our knowledge on the structure, distribution, evolution and mechanism of transposons will continue to provide important contributions to our understanding of host genome functions...
  82. pmc Mobile elements create structural variation: analysis of a complete human genome
    Jinchuan Xing
    Department of Human Genetics, Eccles Institute of Human Genetics, University of Utah, Salt Lake City, Utah 84109, USA
    Genome Res 19:1516-26. 2009
    ....
  83. ncbi A Tn7-based broad-range bacterial cloning and expression system
    Kyoung Hee Choi
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
    Nat Methods 2:443-8. 2005
    ..pestis. This system will thus have widespread biomedical and environmental applications, especially in environments where plasmids and antibiotic selection are not feasible, namely in plant and animal models or biofilms...
  84. ncbi Insertion sequences in prokaryotic genomes
    Patricia Siguier
    Laboratoire de Microbiologie et Génétique Moléculaires UMR5100 CNRS Campus Université Paul Sabatier 118, route de Narbonne, F 31062 Toulouse Cedex, France
    Curr Opin Microbiol 9:526-31. 2006
    ..In addition, defective (non- autonomous) elements, which can be complemented by related active elements in the same cell, are often overlooked in genome annotations but also contribute to the evolution of genome organisation...
  85. pmc Chromosomal transposition of PiggyBac in mouse embryonic stem cells
    Wei Wang
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, United Kingdom
    Proc Natl Acad Sci U S A 105:9290-5. 2008
    ..The comprehensive information from this study should facilitate further exploration of the potential of PB and SB DNA transposons in mammalian genetics...
  86. ncbi Improved detection and annotation of transposable elements in sequenced genomes using multiple reference sequence sets
    Nicolas Buisine
    Unite de Recherche en Genomique Vegetale, INRA UMR1165 CNRS UMR8114 Université d Evry Val d Essonne, 2 rue Gaston Cremieux, 91057 Evry, France
    Genomics 91:467-75. 2008
    ..Significantly, we find that TE detection could be readily optimized using only two reference sets, one containing true consensus sequences and the other mosaic sequences that capture the structural diversity of TE copies within a family...
  87. pmc Pseudogene-derived small interfering RNAs regulate gene expression in mouse oocytes
    Oliver H Tam
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences and Howard Hughes Medical Institute, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 453:534-8. 2008
    ..Our findings indicate a function for pseudogenes in regulating gene expression by means of the RNA interference pathway and may, in part, explain the evolutionary pressure to conserve argonaute-mediated catalysis in mammals...
  88. pmc Repeated horizontal transfer of a DNA transposon in mammals and other tetrapods
    John K Pace
    Department of Biology, University of Texas at Arlington, P O Box 19498, Arlington, TX 76019, USA
    Proc Natl Acad Sci U S A 105:17023-8. 2008
    ..In summary, HT of DNA transposons has contributed significantly to shaping and diversifying the genomes of multiple mammalian and tetrapod species...
  89. ncbi Revisiting horizontal transfer of transposable elements in Drosophila
    E L S Loreto
    UFSM Universidade Federal de Santa Maria, Santa Maria, RS, Brazil
    Heredity (Edinb) 100:545-54. 2008
    ..We discuss the methods used to infer HT, their limits and the putative vectors of transposable elements. We outline all the alternative hypotheses and ask how we can be almost certain that phylogenetic inconsistencies are due to HT...
  90. ncbi Tuned for transposition: molecular determinants underlying the hyperactivity of a Stowaway MITE
    Guojun Yang
    Department of Plant Biology, University of Georgia, Athens, GA 30602, USA
    Science 325:1391-4. 2009
    ..The MITE contains internal sequences that enhance transposition. These findings suggest that MITEs achieve high transposition activity by scavenging transposases encoded by distantly related and self-restrained autonomous elements...
  91. ncbi Small RNA-mediated quiescence of transposable elements in animals
    Kuniaki Saito
    Department of Molecular Biology, Keio University School of Medicine, Tokyo 160 8582, Japan
    Dev Cell 19:687-97. 2010
    ....
  92. pmc Selfish genetic elements, genetic conflict, and evolutionary innovation
    John H Werren
    Department of Biology, University of Rochester, Rochester, NY 14627, USA
    Proc Natl Acad Sci U S A 108:10863-70. 2011
    ..The dynamics of SGEs are also considered, including possible "evolutionary functions" of SGEs...
  93. ncbi Bursts of retrotransposition reproduced in Arabidopsis
    Sayuri Tsukahara
    Department of Integrated Genetics, National Institute of Genetics, Yata 1111, Mishima, Shizuoka 411 8540, Japan
    Nature 461:423-6. 2009
    ....
  94. pmc Identification of miniature inverted-repeat transposable elements (MITEs) and biogenesis of their siRNAs in the Solanaceae: new functional implications for MITEs
    Hanhui Kuang
    Plant Gene Expression Center, Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, California 94720, USA
    Genome Res 19:42-56. 2009
    ..We provide evidence that DICER-LIKE4 (DCL4) may also play a role in MITE siRNA generation in the Solanaceae...
  95. pmc Extensive demethylation of repetitive elements during seed development underlies gene imprinting
    Mary Gehring
    Howard Hughes Medical Institute HHMI, Fred Hutchinson Cancer Research Center FHCRC, 1100 Fairview Avenue North, Seattle, WA 98109, USA
    Science 324:1447-51. 2009
    ..These data suggest that imprinting in plants evolved from targeted methylation of transposable element insertions near genic regulatory elements followed by positive selection when the resulting expression change was advantageous...
  96. pmc A comprehensive transposon mutant library of Francisella novicida, a bioweapon surrogate
    Larry A Gallagher
    Department of Genome Sciences, University of Washington, Campus Box 355065, 1705 NE Pacific Street, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 104:1009-14. 2007
    ..The library provides a resource for virtually complete identification of genes involved in virulence and other nonessential processes...
  97. ncbi Isolation of human iPS cells using EOS lentiviral vectors to select for pluripotency
    Akitsu Hotta
    Developmental and Stem Cell Biology Program, Toronto, Ontario, Canada
    Nat Methods 6:370-6. 2009
    ..Finally, we used EOS selection to establish Rett syndrome-specific mouse and human iPS cell lines with known mutations in MECP2...
  98. pmc ISCR elements: novel gene-capturing systems of the 21st century?
    Mark A Toleman
    Department of Cellular and Molecular Medicine, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    Microbiol Mol Biol Rev 70:296-316. 2006
    ..It appears that ISCR elements have now firmly established themselves within that regimen...
  99. ncbi Transposition of cloned P elements into Drosophila germ line chromosomes
    A C Spradling
    Science 218:341-7. 1982
    ..Thus P elements can efficiently and selectively transpose from extrachromosomal DNA to the DNA of germ line chromosomes in Drosophila embryos. These observations provide the basis for efficient DNA-mediated gene transfer in Drosophila...
  100. pmc Plasticity in patterns of histone modifications and chromosomal proteins in Drosophila heterochromatin
    Nicole C Riddle
    Department of Biology, Washington University St Louis, Missouri 63130, USA
    Genome Res 21:147-63. 2011
    ..This comprehensive analysis provides a foundation for future studies of gene activity and chromosomal functions that are influenced by or dependent upon heterochromatin...

Research Grants70

  1. Selenium, Aminothiols, and Radiation
    Alan Diamond; Fiscal Year: 2004
    ..Collectively, these studies will provide new insight on how selenium protects cells from radiation-induced mutagenesis and may lead to novel approaches to increase the beneficial properties of this nutrient. ..
  2. Genetic Manipulation of Insects
    DAVID O BROCHTA; Fiscal Year: 2004
    ..The meeting is an uncommon opportunity for graduate students and postdoctoral fellows to obtain a high level synopsis of the field of insect molecular genetics and to make important career-advancing interactions. ..
  3. HOBO-LIKE ELEMENTS IN INSECTS
    DAVID O BROCHTA; Fiscal Year: 2008
    ..gambiae populations, 3) develop Herves into a mosquito gene vector. These aims will be accomplished by performing a combination of laboratory and field-based studies using molecular and population genetics tools. ..
  4. Tn5 as an insect genomics tool
    DAVID O BROCHTA; Fiscal Year: 2002
    ..gambiae make this is a high risk project but one whose impact on the field of medical entomology and efforts to control the transmission of malaria will be enormous if it is successful. ..
  5. MECHANISMS OF MYCOPLASMA-INDUCED ARTHRITIS
    Kevin Dybvig; Fiscal Year: 2009
    ..An understanding of the inability of 158-1 to resist host defenses may offer an unique opportunity to explore interactions between the mycoplasma and innate immunity. ..
  6. Tandemly Repetitive Proteins in Mycoplasmas
    KEVIN F DYBVIG; Fiscal Year: 2010
    ..By understanding the role of repetitive proteins in the disease process, it is anticipated that improved measures to control the infections of these significant human pathogens will be developed. ..
  7. YEAST GENETIC INTERACTION MAP
    JEF BOEKE; Fiscal Year: 2008
    ..Thus, to our knowledge, we are the only group that can complete the remaining 98% of the yeast synthetic lethal interaction map. [unreadable] [unreadable] [unreadable]..
  8. Genetic methods using the Tol2 transposable element
    Koichi Kawakami; Fiscal Year: 2006
    ..abstract_text> ..
  9. TRANSCRIPTIONAL SILENCING MECHANISMS
    JEF BOEKE; Fiscal Year: 2005
    ..We will use the tools of molecular genetics, biochemistry and structural biology to carry out a comprehensive analysis of the molecular mechanisms involved in silencing. ..
  10. Identification of Tetrahymena DNA Rearrangement Genes
    DOUGLAS LEE CHALKER; Fiscal Year: 2010
    ..abstract_text> ..
  11. Gene drive potential of transposable elements in Anopheles gambiae.
    DAVID A O apos BROCHTA; Fiscal Year: 2010
    ..gambiae will be known. This project involves the use of Animals but does not involve Human Subjects. ..
  12. GENETIC RECOMBINATION IN MYCOPLASMAS
    Kevin Dybvig; Fiscal Year: 2004
    ..In aim 2, mutants with defects in putative DNA recombinases and V-I protein production will be examined to study the mechanisms of gene rearrangements and phenotypic switching. ..
  13. ANTIGENIC VARIATION IN MYCOPLASMAS
    Kevin Dybvig; Fiscal Year: 2004
    ....
  14. Global Analysis of the Rickettsia prowazekii Proteome
    David Wood; Fiscal Year: 2007
    ..Identification of differences in rickettsial protein expression will increase our understanding of this dynamic process as well as identify potentially new targets for vaccines and diagnostic reagents. ..
  15. Adenoviral GFP targeting of metastatic human tumors using multiple delivery route
    Hiroyuki Kishimoto; Fiscal Year: 2008
    ..Having obtained these results, we can move forward in the Phase II grant application with experiments to bring OBP-401 to the clinic for use in cancer surgery in human patients. [unreadable] [unreadable] [unreadable]..
  16. Biochemical analysis of Pol II elongation complexes and mechanism
    CRAIG KAPLAN; Fiscal Year: 2007
    ..Insights into Pol II function will allow a better understanding of how Pol II may be misregulated in disease states in other species, such as humans. [unreadable] [unreadable] [unreadable]..
  17. Novel Strategy for Animal Model Testing of HCMV Vaccines
    Mark Schleiss; Fiscal Year: 2007
    ..These studies will represent the first efficacy test of a human CMV subunit vaccine in a small animal model of congenital CMV infection. [unreadable] [unreadable]..
  18. Selfish gene and genetic control of vector-borne diseases
    ZACH ADELMAN; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  19. Manipulating the zebrafish genome through modulation of conserved helicases
    Shannon Fisher; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  20. Dual-color tumor-host imaging models
    Meng Yang; Fiscal Year: 2007
    ..These models have significant commercial potential for discovery and development of stroma-targeted and anti-angiogenesis drugs. [unreadable] [unreadable] [unreadable]..
  21. Targeted tumoricidal bacteria
    Ming Zhao; Fiscal Year: 2007
    ..Future human trials of the tumor-killing bacteria can be held after the Phase I and Phase II grant periods are completed. [unreadable] [unreadable] [unreadable]..
  22. LcrV Plague Vaccine with Altered Immune Modulatory Properties
    Olaf Schneewind; Fiscal Year: 2008
    ..unreadable] [unreadable] [unreadable]..
  23. Comparative Evolutionary Genomics and Infectious Disease
    Jane Carlton; Fiscal Year: 2010
    ....
  24. Host-Pathogen Interactions in Invasive Aspergillosis
    WILLIAM CHARLES NIERMAN; Fiscal Year: 2010
    ..necessary to maintain protective immunity against the invading fungus. This hithertn inaccessible ... in order to >.E =.m m'- N'3 ..' 'C3 _.n '-' a.. m._ o'< m- ..
  25. RNAi as a modulator of arbovirus vector competence in transgenic Aedes aegypti
    ALEXANDER WILLIAM EDUARD FRANZ; Fiscal Year: 2010
    ..aegypti when they are not controlled by the mosquito's RNAi response. The proposed research will help to reveal the impact of RNAi as an antiviral defense mechanism in the midgut of Ae. aegypti. ..
  26. A novel transgenic silkworm system for recombinant glycoprotein production
    Donald L Jarvis; Fiscal Year: 2010
    ..Finally, these results will be of great interest to bioengineers working to overcome the evolutionary limitations of lower eukaryotic systems for recombinant glycoprotein production. ..
  27. Engineering enzymes for anti-tumor suicide gene therapy
    Barry L Stoddard; Fiscal Year: 2010
    ..Our hypothesis is that decitabine should ultimately couple with engineered enzyme variants to yield improvements in the performance of dCK, due to the lack of this activity in non-cancerous tissues. ..
  28. Tol2-mediated gene and enhancer trapping in Xenopus tropicalis
    Paul E Mead; Fiscal Year: 2010
    ..We are currently characterizing the novel integration events in the progeny from the other remobilized F2 frogs. We anticipate submitting a manuscript describing this data soon. ..
  29. REGULATION OF GERMLINE STEM CELL DIVISION IN DROSOPHILA
    Haifan Lin; Fiscal Year: 2010
    ..4. Identify the small RNA targets of PIWI and characterize their function in the epigenetic regulation of stem and niche cell genomes. ..
  30. Genetics of Wing and Cell-Size Evolution in Nasonia
    John Werren; Fiscal Year: 2008
    ....
  31. Quality Control of Protein Translation
    A Wali Karzai; Fiscal Year: 2008
    ..abstract_text> ..
  32. Systematic Genetic Analysis of Fungal Virulence
    Hiten Madhani; Fiscal Year: 2009
    ..By systematically identifying molecules produced by C. neoformans important for virulence and by understanding how they function, we hope to lay the groundwork for the discovery of new therapies to treat fungal infections. ..
  33. Development and evaluation of subunit vaccine CmeC against Campylobacter jejuni
    Jun Lin; Fiscal Year: 2008
    ..S. The proposed research will provide novel information on the development of effective vaccine against C. jejuni to protect public health. [unreadable] [unreadable] [unreadable]..
  34. Brain Tumor Stem Cell-targeted Gene Therapy
    JOHN OHLFEST; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  35. Imageable tumor-targeting bacteria
    Ming Zhao; Fiscal Year: 2006
    ..unreadable] [unreadable] [unreadable]..
  36. Male Fertility & Protein Expression in Spermatogenesis
    PAUL RICK; Fiscal Year: 2004
    ..abstract_text> ..
  37. SCREEN FOR TUMOR SUPPRESSORS ON MOUSE CHROMOSOME 4
    Allan Bradley; Fiscal Year: 2001
    ..The pro-viral tag will facilitate the molecular identification of insertionally inactivated tumor suppressor genes in these cell lines. ..
  38. ATP-DEPENDENT PROTEIN KINASES IN GRAM-POSITIVE BACTERIA
    Milton Saier; Fiscal Year: 2004
    ..abstract_text> ..
  39. BIOSYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN
    PAUL RICK; Fiscal Year: 2004
    ..Such information will also provide a rationale for the development of new antimicrobial agents. ..
  40. Gene Activation by Remote Transcriptional Enhancers
    Dale Dorsett; Fiscal Year: 2004
    ..This will help illuminate the basic mechanisms underlying gene expression defects that occur in some human diseases. ..
  41. Fourth Biennial Arthritis Research Conference
    MARY CROW; Fiscal Year: 2003
    ..abstract_text> ..
  42. Microarray Expression Analysis of Burkholderia Mallei
    William Nierman; Fiscal Year: 2003
    ..Function and virulence validation of the identified virulence genes will be explored by construction and phenotype analysis of targeted knockout strains. ..
  43. The Transcriptome of Pathogenic Aspergillus Fumigatus
    William Nierman; Fiscal Year: 2003
    ..abstract_text> ..
  44. POLYMORPHISM/DIVERGENCE IN DROSOPHILA PATHOGEN DEFENSES
    Andrew Clark; Fiscal Year: 2002
    ....
  45. Inhibitors of 5alpha-reductase for acne therapy
    Lingna Li; Fiscal Year: 2002
    ..PROPOSED COMMERCIAL APPLICATIONS: Liposomal 4-MA will be developed as a topical selectively targeted therapeutic for acne for which they should be a very market. ..
  46. ROLE OF FRIZZLED RECEPTORS AND EMBRYOGENESIS
    Stephen Ekker; Fiscal Year: 2001
    ..Using these tools, we will identify the signaling cascades mediated by this family of receptor- like molecules in an attempt to understand in a broader context the role this new gene family plays in development. ..
  47. VIRULENCE FACTORS OF GROUP B STREPTOCOCCI
    John Bohnsack; Fiscal Year: 2001
    ..This approach will ultimately contribute to understanding the molecular basis of disease caused by GBS, and help devise strategies for preventing and treating this important cause of serious human neonatal bacterial infection. ..
  48. Gordon Conf. on Cellular and Molecular Fungal Biology
    James Kronstad; Fiscal Year: 2004
    ..In summary, the conference provides a unique opportunity to examine the breadth of fungal biology in a small meeting format that encourages in-depth discussion among the attendees. ..
  49. Structural studies of blood coagulation proteins
    Barry Stoddard; Fiscal Year: 2006
    ..The third specific aim for this project is to determine the structure of the N-terminal, D'-D3 domain from von Willebrand Factor, alone and/or in complex with factor VIII heterodimer. ..
  50. Enzyme therapy for hyperhomocysteinemia
    Yuying Tan; Fiscal Year: 2005
    ..Phase II results will lead to an IND for MEGC-PEG-rMETase for end stage renal disease patients and other patients with intractable hyperhomocysteinemia and high cardiovascular disease mortality. ..
  51. Tangle Analysis of DNA Recombinases & Related Proteins
    ISABEL DARCY; Fiscal Year: 2005
    ..Apart from the recombination systems investigated in this proposal, the tangle-analysis tools that we develop will be extended to other recombinases and used to investigate related systems such as topoisomerases. ..
  52. MOLECULAR ANALYSIS OF THE INSERTION ELEMENT IS903
    KEITH DERBYSHIRE; Fiscal Year: 2004
    ..3. To establish biochemical assays that will identify specific transposase-DNA contacts, define stable domains of the protein and facilitate the development of an in vitro transposition system. ..
  53. Plant Transposases and their MITEs
    Susan Wessler; Fiscal Year: 2007
    ..As such, knowledge of TE-mediated mechanisms of diversification are intimately involved in improving human health and understanding how plant and animal genomes evolve. ..
  54. RECOMBINATION MECHANISMS IN YEAST CELL DIFFERENTIATION
    James Haber; Fiscal Year: 2006
    ..abstract_text> ..
  55. "Vivax Malaria Research: 2005 and Beyond"
    Jane Carlton; Fiscal Year: 2005
    ..vivax with P. falciparum, epidemiology and diversity studies, vectors and transmission dynamics, and a bioinformatics workshop for researchers and students to learn to manipulate Plasmodium sequence data. ..
  56. Large-scale Gene Deletion in Infectious M. tuberculosis
    John Ho; Fiscal Year: 2006
    ..The proposed and planned future studies may further the control of an unrecognized emerging epidemic and identify both host and pathogen factors that mediate clinical and pathogenic variability. ..
  57. ROLE OF RHOMBOID HOMOLOGUES IN MYCOBACTERIA SIGNALING
    Moses Joloba; Fiscal Year: 2006
    ..This will expand our understanding of mycobacterial biology and may provide novel candidate vaccines and drug targets. ..
  58. CELL TO CELL SIGNALING IN MYCOBACTERIA
    MOSES LUTAAKOME JOLOBA; Fiscal Year: 2010
    ..This will enrich our knowledge on mycobacterial biology and may reveal novel drug and vaccine targets. ..
  59. Identification of Rheumatic Disease Genes
    MARY CROW; Fiscal Year: 2004
    ..abstract_text> ..
  60. Interferon in Systemic Lupus Erythematosus
    Mary K Crow; Fiscal Year: 2010
    ..Elucidation of this important pathway should lead to more targeted modulation of disease mediators in systemic autoimmune diseases. ..
  61. RNA Polymerase III transcription in Leishmania major
    SANTIAGO MARTINEZ CALVILLO; Fiscal Year: 2009
    ..The tandem affinity purification (TAP-tag) method will be used to purify Pol III transcriptional complexes. The protein components in these complexes will be identified by mass-spectrometry analysis. ..
  62. Improved Brucella Vaccine Strains
    Thomas Ficht; Fiscal Year: 2009
    ..abstract_text> ..
  63. Secretion systems for anti-malarial bacterial strains
    David Lampe; Fiscal Year: 2006
    ..Both Type I and Type II secretion systems will be developed and tested. [unreadable] [unreadable] [unreadable]..
  64. COOPERATING GENETIC EVENTS IN MYELOID LEUKEMIA DEVELOPME
    DAVID LARGAESPADA; Fiscal Year: 2002
    ..It is the goal of this proposal to define the overlap between the oncogenic effects of these mutations and discover other proteins involved in this pathway. ..
  65. Essential role of biotin in cell proliferation
    Janos Zempleni; Fiscal Year: 2004
    ..The basic knowledge generated may well be relevant to teratogenesis and immune dysfunction caused by biotin deficiency. ..
  66. Molecular Epidemiology of Community-Associated MRSA
    SANJAY SHUKLA; Fiscal Year: 2008
    ..We will determine the presence or absence of 40 different virulence factor genes in all 320 isolates listed in aim 2 and determine evolution of virulence factor genes in contemporary CA-MRSA. ..
  67. Epigenetic effects of biotin on activation of endogenous viral sequences
    Janos Zempleni; Fiscal Year: 2007
    ..These studies are likely to identify means by which manipulation of the environmental factor "diet" increases genomic stability and decreases cancer risk. [unreadable] [unreadable] [unreadable] [unreadable]..
  68. NF1 GENE IN MYELOID LEUKEMIA AND CYTOKINE SIGNALING
    DAVID LARGAESPADA; Fiscal Year: 2002
    ....
  69. QUINOLONE RESISTANCE MECHANISMS IN STAPHYLOCOCCUS AUREUS
    David Hooper; Fiscal Year: 2007
    ..abstract_text> ..