dna transposable elements

Summary

Summary: Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.

Top Publications

  1. doi The B73 maize genome: complexity, diversity, and dynamics
    Patrick S Schnable
    Center for Plant Genomics, Iowa State University, Ames, IA 50011, USA
    Science 326:1112-5. 2009
  2. ncbi Initial sequencing and analysis of the human genome
    E S Lander
    Whitehead Institute for Biomedical Research, Center for Genome Research, Cambridge, Massachusetts 02142, USA
    Nature 409:860-921. 2001
  3. ncbi Genes required for mycobacterial growth defined by high density mutagenesis
    Christopher M Sassetti
    Department of Immunology and Infectious Diseases, Harvard School of Public Health, Harvard Medical School, Boston, MA 02115, USA
    Mol Microbiol 48:77-84. 2003
  4. ncbi A unified classification system for eukaryotic transposable elements
    Thomas Wicker
    Institute of Plant Biology, University Zurich, Zollikerstrasse 107, Ch 8008 Zurich, Switzerland
    Nat Rev Genet 8:973-82. 2007
  5. ncbi Repbase Update, a database of eukaryotic repetitive elements
    J Jurka
    Genetic Information Research Institute, Mountain View, CA 94043, USA
    Cytogenet Genome Res 110:462-7. 2005
  6. ncbi Discrete small RNA-generating loci as master regulators of transposon activity in Drosophila
    Julius Brennecke
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences and Howard Hughes Medical Institute, Cold Spring Harbor, NY 11724, USA
    Cell 128:1089-103. 2007
  7. pmc Genome sequence of Aedes aegypti, a major arbovirus vector
    Vishvanath Nene
    Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Science 316:1718-23. 2007
  8. doi Horizontal gene transfer in eukaryotic evolution
    Patrick J Keeling
    Department of Botany, University of British Columbia, Vancouver, British Columbia V6T 1Z, Canada
    Nat Rev Genet 9:605-18. 2008
  9. pmc Repetitive elements may comprise over two-thirds of the human genome
    A P Jason De Koning
    Department of Biochemistry and Molecular Genetics, School of Medicine, University of Colorado, Aurora, Colorado, USA
    PLoS Genet 7:e1002384. 2011
  10. pmc The TIGR Rice Genome Annotation Resource: improvements and new features
    Shu Ouyang
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Nucleic Acids Res 35:D883-7. 2007

Detail Information

Publications252 found, 100 shown here

  1. doi The B73 maize genome: complexity, diversity, and dynamics
    Patrick S Schnable
    Center for Plant Genomics, Iowa State University, Ames, IA 50011, USA
    Science 326:1112-5. 2009
    ..These analyses inform and set the stage for further investigations to improve our understanding of the domestication and agricultural improvements of maize...
  2. ncbi Initial sequencing and analysis of the human genome
    E S Lander
    Whitehead Institute for Biomedical Research, Center for Genome Research, Cambridge, Massachusetts 02142, USA
    Nature 409:860-921. 2001
    ..We also present an initial analysis of the data, describing some of the insights that can be gleaned from the sequence...
  3. ncbi Genes required for mycobacterial growth defined by high density mutagenesis
    Christopher M Sassetti
    Department of Immunology and Infectious Diseases, Harvard School of Public Health, Harvard Medical School, Boston, MA 02115, USA
    Mol Microbiol 48:77-84. 2003
    ....
  4. ncbi A unified classification system for eukaryotic transposable elements
    Thomas Wicker
    Institute of Plant Biology, University Zurich, Zollikerstrasse 107, Ch 8008 Zurich, Switzerland
    Nat Rev Genet 8:973-82. 2007
    ..The system and nomenclature is kept up to date at the WikiPoson web site...
  5. ncbi Repbase Update, a database of eukaryotic repetitive elements
    J Jurka
    Genetic Information Research Institute, Mountain View, CA 94043, USA
    Cytogenet Genome Res 110:462-7. 2005
    ..Sequences from Repbase Update are used to screen and annotate repetitive elements using programs such as Censor and RepeatMasker. Repbase Update is available on the worldwide web at http://www.girinst.org/Repbase_Update.html...
  6. ncbi Discrete small RNA-generating loci as master regulators of transposon activity in Drosophila
    Julius Brennecke
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences and Howard Hughes Medical Institute, Cold Spring Harbor, NY 11724, USA
    Cell 128:1089-103. 2007
    ..Thus, sense piRNAs, formed following cleavage of transposon mRNAs may enhance production of antisense piRNAs, complementary to active elements, by directing cleavage of transcripts from master control loci...
  7. pmc Genome sequence of Aedes aegypti, a major arbovirus vector
    Vishvanath Nene
    Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Science 316:1718-23. 2007
    ..An increase in genes encoding odorant binding, cytochrome P450, and cuticle domains relative to An. gambiae suggests that members of these protein families underpin some of the biological differences between the two mosquito species...
  8. doi Horizontal gene transfer in eukaryotic evolution
    Patrick J Keeling
    Department of Botany, University of British Columbia, Vancouver, British Columbia V6T 1Z, Canada
    Nat Rev Genet 9:605-18. 2008
    ..Major recent trends include the important role of HGT in adaptation to certain specialized niches and the highly variable impact of HGT in different lineages...
  9. pmc Repetitive elements may comprise over two-thirds of the human genome
    A P Jason De Koning
    Department of Biochemistry and Molecular Genetics, School of Medicine, University of Colorado, Aurora, Colorado, USA
    PLoS Genet 7:e1002384. 2011
    ..These results highlight the need for combined, probabilistic genome annotation approaches and suggest that the human genome consists of substantially more repetitive sequence than previously believed...
  10. pmc The TIGR Rice Genome Annotation Resource: improvements and new features
    Shu Ouyang
    The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Nucleic Acids Res 35:D883-7. 2007
    ..Enhanced data access is available through web interfaces, FTP downloads and a Data Extractor tool developed in order to support discrete dataset downloads...
  11. pmc Transposable elements and the evolution of regulatory networks
    CEDRIC FESCHOTTE
    Department of Biology, Life Science Building, Box 19498, University of Texas, Arlington, Texas 76019, USA
    Nat Rev Genet 9:397-405. 2008
    ..Here I revisit and expand earlier models and propose that genomic repeats, and in particular transposable elements, have been a rich source of material for the assembly and tinkering of eukaryotic gene regulatory systems...
  12. ncbi The complete genome sequence of Escherichia coli K-12
    F R Blattner
    Laboratory of Genetics, University of Wisconsin Madison, 445 Henry Mall, Madison, WI 53706, USA
    Science 277:1453-62. 1997
    ..The genome also contains insertion sequence (IS) elements, phage remnants, and many other patches of unusual composition indicating genome plasticity through horizontal transfer...
  13. pmc DNA transposons and the evolution of eukaryotic genomes
    CEDRIC FESCHOTTE
    Department of Biology, University of Texas, Arlington, TX 76019, USA
    Annu Rev Genet 41:331-68. 2007
    ..Finally, we highlight how the distinctive biological features of DNA transposons have contributed to shape genome architecture and led to the emergence of genetic innovations in different eukaryotic lineages...
  14. doi Transposon-mediated rewiring of gene regulatory networks contributed to the evolution of pregnancy in mammals
    Vincent J Lynch
    Department of Ecology and Evolutionary Biology and Yale Systems Biology Institute, Yale University, New Haven, Connecticut, USA
    Nat Genet 43:1154-9. 2011
    ....
  15. doi Transposable elements have rewired the core regulatory network of human embryonic stem cells
    Galih Kunarso
    Computational and Mathematical Biology, Genome Institute of Singapore, Singapore, Singapore
    Nat Genet 42:631-4. 2010
    ..These data indicate that species-specific transposable elements have substantially altered the transcriptional circuitry of pluripotent stem cells...
  16. pmc Epigenetic reprogramming and small RNA silencing of transposable elements in pollen
    R Keith Slotkin
    Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Cell 136:461-72. 2009
    ..We propose a conserved role for reprogramming in germline companion cells, such as nurse cells in insects and vegetative nuclei in plants, to reveal intact TEs in the genome and regulate their activity in gametes...
  17. doi Genome sequence and analysis of the Irish potato famine pathogen Phytophthora infestans
    Brian J Haas
    Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02141, USA
    Nature 461:393-8. 2009
    ..infestans genome. This probably plays a crucial part in the rapid adaptability of the pathogen to host plants and underpins its evolutionary potential...
  18. pmc A piRNA pathway primed by individual transposons is linked to de novo DNA methylation in mice
    Alexei A Aravin
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Mol Cell 31:785-99. 2008
    ..This implicates piRNAs as specificity determinants of DNA methylation in germ cells...
  19. pmc Annotation, submission and screening of repetitive elements in Repbase: RepbaseSubmitter and Censor
    Oleksiy Kohany
    Genetic Information Research Institute, 1925 Landings Drive, Mountain View, CA 94043, USA
    BMC Bioinformatics 7:474. 2006
    ..Updating and maintenance of the database requires specialized tools, which we have created and made available for use with Repbase, and which may be useful as a template for other curated databases...
  20. ncbi P[acman]: a BAC transgenic platform for targeted insertion of large DNA fragments in D. melanogaster
    Koen J T Venken
    Program in Developmental Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Science 314:1747-51. 2006
    ..This transgenesis platform should greatly facilitate structure/function analyses of most Drosophila genes...
  21. pmc ISfinder: the reference centre for bacterial insertion sequences
    P Siguier
    Laboratoire de Microbiologie et Genetique Moleculaires, C N R S 118, route de Narbonne, F 31062 Toulouse Cedex, France
    Nucleic Acids Res 34:D32-6. 2006
    ..Finally, this database is currently recommended by several microbiology journals for registration of new IS elements before their publication...
  22. pmc Simultaneous assay of every Salmonella Typhi gene using one million transposon mutants
    Gemma C Langridge
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, United Kingdom
    Genome Res 19:2308-16. 2009
    ..Typhi in the gall bladder. This screen validated our hypothesis that we can simultaneously assay every gene in the genome to identify niche-specific essential genes...
  23. pmc Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon
    Kosuke Yusa
    Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
    Nat Methods 6:363-9. 2009
    ..iPSCs fulfilled all criteria of pluripotency, such as pluripotency gene expression, teratoma formation and contribution to chimeras. piggyBac transposon-based reprogramming may be used to generate therapeutically applicable iPSCs...
  24. ncbi Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice
    Sheng Ding
    Institute of Developmental Biology and Molecular Medicine, School of Life Sciences, Fudan University, 220 Handan Road, Shanghai 200433, China
    Cell 122:473-83. 2005
    ..These data provide a first and critical step toward a highly efficient transposon system for a variety of genetic manipulations including transgenesis and insertional mutagenesis in mice and other vertebrates...
  25. ncbi An active DNA transposon family in rice
    Ning Jiang
    Department of Plant Biology, University of Georgia, Athens, Georgia 30602, USA
    Nature 421:163-7. 2003
    ..Since the domestication of rice mPing MITEs have been amplified preferentially in cultivars adapted to environmental extremes-a situation that is reminiscent of the genomic shock theory for transposon activation...
  26. pmc Promiscuous DNA: horizontal transfer of transposable elements and why it matters for eukaryotic evolution
    Sarah Schaack
    Department of Biology, University of Texas at Arlington, Arlington, TX 76019, USA
    Trends Ecol Evol 25:537-46. 2010
    ..We argue that the introduction of transposable elements by horizontal transfer in eukaryotic genomes has been a major force propelling genomic variation and biological innovation...
  27. pmc Epigenetic silencing of transposable elements: a trade-off between reduced transposition and deleterious effects on neighboring gene expression
    Jesse D Hollister
    Department of Ecology and Evolutionary Biology, University of Californina, Irvine, Irvine, California 92697 2525, USA
    Genome Res 19:1419-28. 2009
    ..This mechanism implies an evolutionary tradeoff in which the benefit of TE silencing imposes a fitness cost via deleterious effects on the expression of nearby genes...
  28. doi Origin of primate orphan genes: a comparative genomics approach
    Macarena Toll-Riera
    Evolutionary Genomics Group, Biomedical Informatics Research Programme, Fundació Institut Municipal d Investigació Mèdica, Barcelona, Spain
    Mol Biol Evol 26:603-12. 2009
    ..This indicates frequent recruitment of TEs as part of novel genes. Finally, we also obtain evidence that a small fraction of primate orphan genes, around 5.5%, might have originated de novo from mammalian noncoding genomic regions...
  29. doi Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable gene transfer in vertebrates
    Lajos Mátés
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Nat Genet 41:753-61. 2009
    ....
  30. pmc An epigenetic role for maternally inherited piRNAs in transposon silencing
    Julius Brennecke
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory CSHL, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Science 322:1387-92. 2008
    ..This indicates that maternally deposited piRNAs are important for mounting an effective silencing response and that a lack of maternal piRNA inheritance underlies hybrid dysgenesis...
  31. ncbi Turning junk into gold: domestication of transposable elements and the creation of new genes in eukaryotes
    Jean Nicolas Volff
    Biofuture Research Group, Physiologische Chemie I, Biozentrum, University of Wurzburg, Am Hubland, D 97074 Wurzburg, Germany
    Bioessays 28:913-22. 2006
    ..Many new candidates for domesticated sequences have been revealed by sequencing projects. Their functional analysis will uncover new aspects of evolutionary alchemy, the turning of junk into gold within genomes...
  32. pmc The struggle for life of the genome's selfish architects
    Aurelie Hua-Van
    Laboratoire Evolution, Génomes, Spéciation, CNRS UPR9034 Université Paris Sud, Gif sur Yvette, France
    Biol Direct 6:19. 2011
    ..We also explore the evolutionary aspects of TEs in the light of post-Darwinian theories of evolution...
  33. pmc A role for host-parasite interactions in the horizontal transfer of transposons across phyla
    Clément Gilbert
    Department of Biology, University of Texas, Arlington, Texas 76019, USA
    Nature 464:1347-50. 2010
    ..Furthermore, the large amount of DNA generated by the amplification of the horizontally transferred transposons supports the idea that the exchange of genetic material between hosts and parasites influences their genomic evolution...
  34. pmc Endogenous siRNAs derived from transposons and mRNAs in Drosophila somatic cells
    Megha Ghildiyal
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Science 320:1077-81. 2008
    ..We propose that endo-siRNAs generated by the fly RNAi pathway silence selfish genetic elements in the soma, much as Piwi-interacting RNAs do in the germ line...
  35. doi A modular master on the move: the Tn916 family of mobile genetic elements
    Adam P Roberts
    Division of Microbial Diseases, UCL Eastman Dental Institute, 256 Gray s Inn Road, London, WC1X 8LD, UK
    Trends Microbiol 17:251-8. 2009
    ..Therefore, the study of this family of mobile genetic elements is essential for a better understanding and control of the current rise of antibiotic resistance among pathogenic bacteria...
  36. pmc piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells
    Knut Woltjen
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada
    Nature 458:766-70. 2009
    ..We anticipate that the unique properties of this virus-independent simplification of iPS cell production will accelerate this field further towards full exploration of the reprogramming process and future cell-based therapies...
  37. ncbi Transposable elements and the epigenetic regulation of the genome
    R Keith Slotkin
    Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nat Rev Genet 8:272-85. 2007
    ..In addition, new insights have been gained into how this silencing has been co-opted to serve essential functions in 'host' cells, highlighting the importance of TEs in the epigenetic regulation of the genome...
  38. doi Epigenetic regulation of transposable elements in plants
    Damon Lisch
    Department of Plant and Microbial Biology, University of California, Berkeley, California 94720, USA
    Annu Rev Plant Biol 60:43-66. 2009
    ..Together, these observations suggest that naturally occurring changes in transposon activity may have had an important impact on the causes and consequences of epigenetic silencing in plants...
  39. pmc Transposable elements and small RNAs contribute to gene expression divergence between Arabidopsis thaliana and Arabidopsis lyrata
    Jesse D Hollister
    Department of Ecology and Evolutionary Biology, University of California, Irvine, CA 92697, USA
    Proc Natl Acad Sci U S A 108:2322-7. 2011
    ..Our results suggest that the efficacy of RNA-directed DNA methylation silencing is lower in A. lyrata, a finding that may shed light on the causes of differential TE proliferation among species...
  40. pmc The genome of the Western clawed frog Xenopus tropicalis
    Uffe Hellsten
    Department of Energy Joint Genome Institute, Walnut Creek, CA 94598, USA
    Science 328:633-6. 2010
    ..The genome exhibits substantial shared synteny with human and chicken over major parts of large chromosomes, broken by lineage-specific chromosome fusions and fissions, mainly in the mammalian lineage...
  41. pmc Dual coding of siRNAs and miRNAs by plant transposable elements
    Jittima Piriyapongsa
    School of Biology, Georgia Institute of Technology, Atlanta, Georgia 30332 0230, USA
    RNA 14:814-21. 2008
    ..The siRNA-to-miRNA evolutionary transition is representative of a number of other regulatory mechanisms that evolved to silence TEs and were later co-opted to serve as regulators of host gene expression...
  42. doi Unexpected consequences of a sudden and massive transposon amplification on rice gene expression
    Ken Naito
    Department of Plant Biology, University of Georgia, Athens, Georgia 30602, USA
    Nature 461:1130-4. 2009
    ..As such, this study provides evidence for models first proposed previously for the involvement of transposable elements and other repetitive sequences in genome restructuring and gene regulation...
  43. pmc Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria
    M Herrero
    GBF National Research Centre for Biotechnology, Braunschweig, Federal Republic of Germany
    J Bacteriol 172:6557-67. 1990
    ..g., in open systems and natural environments), and that do not carry antibiotic resistance markers characteristic of most available cloning vectors (as is currently required of live bacterial vaccines)...
  44. ncbi Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells
    Z Ivics
    Department of Genetics and Cell Biology, University of Minnesota, St Paul 55108 1095, USA
    Cell 91:501-10. 1997
    ..Sleeping Beauty is an active DNA-transposon system from vertebrates for genetic transformation and insertional mutagenesis...
  45. pmc DNA transposon Hermes inserts into DNA in nucleosome-free regions in vivo
    Sunil Gangadharan
    Department of Molecular Biology and Genetics, Division of Biostatistics and Bioinformatics, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 107:21966-72. 2010
    ..We have also discovered a long-range sequence motif that defines S. cerevisiae nucleosome-free regions...
  46. pmc Effector diversification within compartments of the Leptosphaeria maculans genome affected by Repeat-Induced Point mutations
    Thierry Rouxel
    INRA Bioger, UR1290, Avenue Lucien Brétignières, BP 01, Thiverval Grignon F 78850, France
    Nat Commun 2:202. 2011
    ..This genomic environment for effectors promotes rapid sequence diversification and underpins the evolutionary potential of the fungus to adapt rapidly to novel host-derived constraints...
  47. pmc The essential genome of a bacterium
    Beat Christen
    Department of Developmental Biology, Stanford University, Stanford, CA, USA
    Mol Syst Biol 7:528. 2011
    ..The high-resolution strategy used here is applicable to high-throughput, full genome essentiality studies and large-scale genetic perturbation experiments in a broad class of bacterial species...
  48. pmc The carnegie protein trap library: a versatile tool for Drosophila developmental studies
    Michael Buszczak
    Howard Hughes Medical Institute Research Laboratories, Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland 21218, USA
    Genetics 175:1505-31. 2007
    ....
  49. ncbi Mobile elements: drivers of genome evolution
    Haig H Kazazian
    Department of Genetics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Science 303:1626-32. 2004
    ..Although the host can often control their numbers, massive expansions of retrotransposons have been tolerated during evolution. Now mobile elements are becoming useful tools for learning more about genome evolution and gene function...
  50. doi Transposable elements: powerful facilitators of evolution
    Keith R Oliver
    School of Biological Sciences and Biotechnology, Faculty of Sustainability, Environmental and Life Sciences, Murdoch University, Perth, Australia
    Bioessays 31:703-14. 2009
    ..We propose a general and holistic synthesis on how the presence of TEs within genomes makes them flexible and dynamic, so that genomes themselves are powerful facilitators of their own evolution...
  51. pmc A transposon-based genetic screen in mice identifies genes altered in colorectal cancer
    Timothy K Starr
    Department of Genetics, Cell Biology and Development, Center for Genome Engineering, Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA
    Science 323:1747-50. 2009
    ..These genes include APC, PTEN, and SMAD4. The screen also identified 17 candidate genes that had not previously been implicated in CRC, including POLI, PTPRK, and RSPO2...
  52. pmc The catalytic domain of all eukaryotic cut-and-paste transposase superfamilies
    Yao Wu Yuan
    Department of Plant Biology, University of Georgia, Athens, GA 30602, USA
    Proc Natl Acad Sci U S A 108:7884-9. 2011
    Cut-and-paste DNA transposable elements are major components of eukaryotic genomes and are grouped into superfamilies (e.g., hAT, P) based on sequence similarity of the element-encoded transposase...
  53. doi Massive horizontal gene transfer in bdelloid rotifers
    Eugene A Gladyshev
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Science 320:1210-3. 2008
    ..One such gene, apparently of bacterial origin, was overexpressed in Escherichia coli and yielded an active enzyme. The capture and functional assimilation of exogenous genes may represent an important force in bdelloid evolution...
  54. doi Molecular domestication of transposable elements: from detrimental parasites to useful host genes
    L Sinzelle
    Max Delbruck Center for Molecular Medicine, Berlin, Germany
    Cell Mol Life Sci 66:1073-93. 2009
    ..Our knowledge on the structure, distribution, evolution and mechanism of transposons will continue to provide important contributions to our understanding of host genome functions...
  55. pmc Mobile elements create structural variation: analysis of a complete human genome
    Jinchuan Xing
    Department of Human Genetics, Eccles Institute of Human Genetics, University of Utah, Salt Lake City, Utah 84109, USA
    Genome Res 19:1516-26. 2009
    ....
  56. ncbi A Tn7-based broad-range bacterial cloning and expression system
    Kyoung Hee Choi
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
    Nat Methods 2:443-8. 2005
    ..pestis. This system will thus have widespread biomedical and environmental applications, especially in environments where plasmids and antibiotic selection are not feasible, namely in plant and animal models or biofilms...
  57. ncbi Insertion sequences in prokaryotic genomes
    Patricia Siguier
    Laboratoire de Microbiologie et Génétique Moléculaires UMR5100 CNRS Campus Université Paul Sabatier 118, route de Narbonne, F 31062 Toulouse Cedex, France
    Curr Opin Microbiol 9:526-31. 2006
    ..In addition, defective (non- autonomous) elements, which can be complemented by related active elements in the same cell, are often overlooked in genome annotations but also contribute to the evolution of genome organisation...
  58. pmc Chromosomal transposition of PiggyBac in mouse embryonic stem cells
    Wei Wang
    The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, United Kingdom
    Proc Natl Acad Sci U S A 105:9290-5. 2008
    ..The comprehensive information from this study should facilitate further exploration of the potential of PB and SB DNA transposons in mammalian genetics...
  59. doi Improved detection and annotation of transposable elements in sequenced genomes using multiple reference sequence sets
    Nicolas Buisine
    Unite de Recherche en Genomique Vegetale, INRA UMR1165 CNRS UMR8114 Université d Evry Val d Essonne, 2 rue Gaston Cremieux, 91057 Evry, France
    Genomics 91:467-75. 2008
    ..Significantly, we find that TE detection could be readily optimized using only two reference sets, one containing true consensus sequences and the other mosaic sequences that capture the structural diversity of TE copies within a family...
  60. pmc Tracking insertion mutants within libraries by deep sequencing and a genome-wide screen for Haemophilus genes required in the lung
    Jeffrey D Gawronski
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, 55 Lake Avenue North, S6 242, Worcester, MA 01655, USA
    Proc Natl Acad Sci U S A 106:16422-7. 2009
    ....
  61. doi Tuned for transposition: molecular determinants underlying the hyperactivity of a Stowaway MITE
    Guojun Yang
    Department of Plant Biology, University of Georgia, Athens, GA 30602, USA
    Science 325:1391-4. 2009
    ..The MITE contains internal sequences that enhance transposition. These findings suggest that MITEs achieve high transposition activity by scavenging transposases encoded by distantly related and self-restrained autonomous elements...
  62. doi Bursts of retrotransposition reproduced in Arabidopsis
    Sayuri Tsukahara
    Department of Integrated Genetics, National Institute of Genetics, Yata 1111, Mishima, Shizuoka 411 8540, Japan
    Nature 461:423-6. 2009
    ....
  63. pmc Pseudogene-derived small interfering RNAs regulate gene expression in mouse oocytes
    Oliver H Tam
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences and Howard Hughes Medical Institute, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
    Nature 453:534-8. 2008
    ..Our findings indicate a function for pseudogenes in regulating gene expression by means of the RNA interference pathway and may, in part, explain the evolutionary pressure to conserve argonaute-mediated catalysis in mammals...
  64. doi Small RNA-mediated quiescence of transposable elements in animals
    Kuniaki Saito
    Department of Molecular Biology, Keio University School of Medicine, Tokyo 160 8582, Japan
    Dev Cell 19:687-97. 2010
    ....
  65. pmc Selfish genetic elements, genetic conflict, and evolutionary innovation
    John H Werren
    Department of Biology, University of Rochester, Rochester, NY 14627, USA
    Proc Natl Acad Sci U S A 108:10863-70. 2011
    ..The dynamics of SGEs are also considered, including possible "evolutionary functions" of SGEs...
  66. pmc Identification of miniature inverted-repeat transposable elements (MITEs) and biogenesis of their siRNAs in the Solanaceae: new functional implications for MITEs
    Hanhui Kuang
    Plant Gene Expression Center, Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, California 94720, USA
    Genome Res 19:42-56. 2009
    ..We provide evidence that DICER-LIKE4 (DCL4) may also play a role in MITE siRNA generation in the Solanaceae...
  67. pmc Repeated horizontal transfer of a DNA transposon in mammals and other tetrapods
    John K Pace
    Department of Biology, University of Texas at Arlington, P O Box 19498, Arlington, TX 76019, USA
    Proc Natl Acad Sci U S A 105:17023-8. 2008
    ..In summary, HT of DNA transposons has contributed significantly to shaping and diversifying the genomes of multiple mammalian and tetrapod species...
  68. doi Revisiting horizontal transfer of transposable elements in Drosophila
    E L S Loreto
    UFSM Universidade Federal de Santa Maria, Santa Maria, RS, Brazil
    Heredity (Edinb) 100:545-54. 2008
    ..We discuss the methods used to infer HT, their limits and the putative vectors of transposable elements. We outline all the alternative hypotheses and ask how we can be almost certain that phylogenetic inconsistencies are due to HT...
  69. pmc Extensive demethylation of repetitive elements during seed development underlies gene imprinting
    Mary Gehring
    Howard Hughes Medical Institute HHMI, Fred Hutchinson Cancer Research Center FHCRC, 1100 Fairview Avenue North, Seattle, WA 98109, USA
    Science 324:1447-51. 2009
    ..These data suggest that imprinting in plants evolved from targeted methylation of transposable element insertions near genic regulatory elements followed by positive selection when the resulting expression change was advantageous...
  70. pmc A comprehensive transposon mutant library of Francisella novicida, a bioweapon surrogate
    Larry A Gallagher
    Department of Genome Sciences, University of Washington, Campus Box 355065, 1705 NE Pacific Street, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 104:1009-14. 2007
    ..The library provides a resource for virtually complete identification of genes involved in virulence and other nonessential processes...
  71. doi Isolation of human iPS cells using EOS lentiviral vectors to select for pluripotency
    Akitsu Hotta
    Developmental and Stem Cell Biology Program, Toronto, Ontario, Canada
    Nat Methods 6:370-6. 2009
    ..Finally, we used EOS selection to establish Rett syndrome-specific mouse and human iPS cell lines with known mutations in MECP2...
  72. pmc ISCR elements: novel gene-capturing systems of the 21st century?
    Mark A Toleman
    Department of Cellular and Molecular Medicine, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK
    Microbiol Mol Biol Rev 70:296-316. 2006
    ..It appears that ISCR elements have now firmly established themselves within that regimen...
  73. ncbi Transposition of cloned P elements into Drosophila germ line chromosomes
    A C Spradling
    Science 218:341-7. 1982
    ..Thus P elements can efficiently and selectively transpose from extrachromosomal DNA to the DNA of germ line chromosomes in Drosophila embryos. These observations provide the basis for efficient DNA-mediated gene transfer in Drosophila...
  74. pmc Plasticity in patterns of histone modifications and chromosomal proteins in Drosophila heterochromatin
    Nicole C Riddle
    Department of Biology, Washington University St Louis, Missouri 63130, USA
    Genome Res 21:147-63. 2011
    ..This comprehensive analysis provides a foundation for future studies of gene activity and chromosomal functions that are influenced by or dependent upon heterochromatin...
  75. pmc Gene transfer efficiency and genome-wide integration profiling of Sleeping Beauty, Tol2, and piggyBac transposons in human primary T cells
    Xin Huang
    Division of Pediatric Blood and Marrow Transplantation, University of Minnesota Medical School, Minneapolis, Minnesota, USA
    Mol Ther 18:1803-13. 2010
    ....
  76. pmc PiggyBac transposon mutagenesis: a tool for cancer gene discovery in mice
    Roland Rad
    Wellcome Trust Sanger Institute, Genome Campus, Hinxton Cambridge CB10 1SA, UK
    Science 330:1104-7. 2010
    ..Mice with different transposon types, copy numbers, and chromosomal locations support wide applicability...
  77. ncbi SOS response promotes horizontal dissemination of antibiotic resistance genes
    John W Beaber
    Department of Microbiology, Tufts University School of Medicine and Howard Hughes Medical Institute, 136 Harrison Avenue, Boston, Massachusetts 02111, USA
    Nature 427:72-4. 2004
    ..Thus, we present a mechanism by which therapeutic agents can promote the spread of antibiotic resistance genes...
  78. doi Jumping genes and epigenetics: Towards new species
    Rita Rebollo
    CNRS, UMR558, Laboratoire de Biometrie et Biologie Evolutive, Universite Lyon 1, Villeurbanne, France
    Gene 454:1-7. 2010
    ..We propose to focus on recent data that demonstrate the correlation between changes in the epigenetic control of TEs in species/populations and genome evolution...
  79. pmc Critical roles for Dicer in the female germline
    Elizabeth P Murchison
    Cold Spring Harbor Laboratory, Watson School of Biological Sciences and Howard Hughes Medical Institute, Cold Spring Harbor, New York 11724, USA
    Genes Dev 21:682-93. 2007
    ..Our studies identify Dicer as central to a regulatory network that controls oocyte gene expression programs and that promotes genomic integrity in a cell type notoriously susceptible to aneuploidy...
  80. pmc Functions required for extracellular quinolone signaling by Pseudomonas aeruginosa
    Larry A Gallagher
    Department of Genome Sciences, University of Washington, Seattle, Washington 98195, USA
    J Bacteriol 184:6472-80. 2002
    ..Others of the pleiotropic phenazine-minus mutations appear to inactivate novel components of the quorum-sensing regulatory network, including one regulator (np20) previously shown to be required for virulence in neutropenic mice...
  81. pmc The evolution of transposable elements in natural populations of self-fertilizing Arabidopsis thaliana and its outcrossing relative Arabidopsis lyrata
    Steven Lockton
    Department of Ecology and Evolutionary Biology, University of California, Irvine, USA
    BMC Evol Biol 10:10. 2010
    ..We analyzed seven TE families in four natural populations of each species to tease apart the effects of mating system, demography, transposition, and selection in determining patterns of TE diversity...
  82. doi Genome-wide demethylation of Arabidopsis endosperm
    Tzung Fu Hsieh
    Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA
    Science 324:1451-4. 2009
    ..Our findings demonstrate extensive reconfiguration of the endosperm methylation landscape that likely reinforces transposon silencing in the embryo...
  83. pmc Conserved themes in small-RNA-mediated transposon control
    Angelique Girard
    Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA
    Trends Cell Biol 18:136-48. 2008
    ....
  84. doi Transposable elements in the mammalian germline: a comfortable niche or a deadly trap?
    N Zamudio
    Inserm U934 UMR3215, Institut Curie, Paris, France
    Heredity (Edinb) 105:92-104. 2010
    ..An organized cross-talk between PIWI-interacting small RNAs (piRNAs) and various nuclear and cytoplasmic accessories provides this potent and multi-layered response to retrotransposon unleashing in early germ cells...
  85. pmc Megabase level sequencing reveals contrasted organization and evolution patterns of the wheat gene and transposable element spaces
    Frédéric Choulet
    Institut National de la Recherche Agronomique, Universite Blaise Pascal, Unité Mixte de Recherche 1095 Genetics Diversity and Ecophysiology of Cereals, F 63100 Clermont Ferrand, France
    Plant Cell 22:1686-701. 2010
    ....
  86. pmc Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH)
    Roy R Chaudhuri
    Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge, CB3 0ES, UK
    BMC Genomics 10:291. 2009
    ..An important starting point for the development of new antimicrobial drugs is the identification of "essential" genes that are important for bacterial survival and growth...
  87. ncbi The complete sequence of the rice (Oryza sativa L.) mitochondrial genome: frequent DNA sequence acquisition and loss during the evolution of flowering plants
    Y Notsu
    Department of Genetic Diversity, National Institute of Agrobiological Sciences, Kannondai 2 1 2, Tsukuba, Ibaraki 305 8602, Japan
    Mol Genet Genomics 268:434-45. 2002
    ....
  88. doi MUST: a system for identification of miniature inverted-repeat transposable elements and applications to Anabaena variabilis and Haloquadratum walsbyi
    Yong Chen
    Computational Systems Biology Laboratory, Department of Biochemistry and Molecular Biology and Institute of Bioinformatics, University of Georgia, 120 Green Street Athens, GA 30602, USA
    Gene 436:1-7. 2009
    ..bmb.uga.edu/ffzhou/MUST/ (the standalone version is available upon request). Supplementary data associated with this article are available in the online version or at: http://csbl1.bmb.uga.edu/ffzhou/MUST/supp/...
  89. pmc Sequence and comparative analysis of the maize NB mitochondrial genome
    Sandra W Clifton
    Genome Sequencing Center, Washington University School of Medicine, St Louis, Missouri 63108, USA
    Plant Physiol 136:3486-503. 2004
    ..After eliminating genes, introns, ORFs, and plastid-derived DNA, nearly three-fourths of the maize NB mitochondrial genome is still of unknown origin and function...
  90. doi Identifying repeats and transposable elements in sequenced genomes: how to find your way through the dense forest of programs
    E Lerat
    Universite de Lyon, F 6900 Lyon
    Heredity (Edinb) 104:520-33. 2010
    ..However, this makes it essential to verify the results provided by each program independently. The ideal solution would be to test all programs against the same data set to obtain a true comparison of their actual performance...
  91. doi Transposable elements in reptilian and avian (sauropsida) genomes
    D Kordis
    Department of Molecular and Biomedical Sciences, J Stefan Institute, Ljubljana, Slovenia
    Cytogenet Genome Res 127:94-111. 2009
    ..The large amount of recently accumulated genome-wide data on TEs in diverse lineages of sauropsids has provided a remarkable opportunity to review current knowledge about TEs of sauropsids in their genomic context...
  92. pmc Genome-wide patterns of adaptation to temperate environments associated with transposable elements in Drosophila
    Josefa González
    Department of Biology, Stanford University, Stanford, California, United States of America
    PLoS Genet 6:e1000905. 2010
    ..We argue that reverse population genomic analyses, as the one described in this work, are necessary to arrive at a comprehensive picture of adaptation...
  93. pmc The evolution of RNAi as a defence against viruses and transposable elements
    Darren J Obbard
    Institute of Evolutionary Biology, School of Biological Sciences, University of Edinburgh, Ashworth Laboratories, King s Buildings, Edinburgh EH9 3JT, UK
    Philos Trans R Soc Lond B Biol Sci 364:99-115. 2009
    ..Over longer time scales, key RNAi genes are repeatedly duplicated or lost across the metazoan phylogeny, with important implications for RNAi as an immune defence...
  94. pmc Role of conjugative elements in the evolution of the multidrug-resistant pandemic clone Streptococcus pneumoniaeSpain23F ST81
    Nicholas J Croucher
    Wellcome Trust Sanger Institute, Hinxton, Cambridge, Cambridgeshire, United Kingdom
    J Bacteriol 191:1480-9. 2009
    ..However, further sequencing of other pandemic clones will be required to establish whether there are any general attributes shared by these strains that are responsible for their international success...
  95. pmc Population dynamics of PIWI-interacting RNAs (piRNAs) and their targets in Drosophila
    Jian Lu
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853, USA
    Genome Res 20:212-27. 2010
    ....
  96. pmc Copy number variation and transposable elements feature in recent, ongoing adaptation at the Cyp6g1 locus
    Joshua M Schmidt
    Department of Genetics, The University of Melbourne, Parkville, Victoria, Australia
    PLoS Genet 6:e1000998. 2010
    ..Thus the Cyp6g1 locus is a major contributor to DDT resistance in field populations, and evolution at this locus features multiple adaptive steps occurring in rapid succession...
  97. ncbi Conjugative transposons: the tip of the iceberg
    Vincent Burrus
    Laboratoire de Génétique et Microbiologie, UMR INRA UHP 1128, IFR110, Faculte des Sciences et Techniques, Universite Henri Poincare Nancy 1, BP239, 54506 Vandoeuvre les Nancy, France
    Mol Microbiol 46:601-10. 2002
    ..These elements evolve by acquisition or exchanges of modules with various transferable elements including at least ICEs and plasmids. The ICEs are probably widespread among the bacteria...
  98. doi A triptych of the evolution of plant transposable elements
    Maud I Tenaillon
    CNRS, UMR 0320 UMR8120 Génétique Végétale, F 91190 Gif sur Yvette, France
    Trends Plant Sci 15:471-8. 2010
    ..Finally, we focus on the often-ignored third panel of our triptych - the population processes that determine the ultimate evolutionary fate of TE insertions...
  99. ncbi Complete genome sequence of a virulent isolate of Streptococcus pneumoniae
    H Tettelin
    The Institute for Genomic Research (TIGR, 9712 Medical Center Drive, Rockville, MD 20850, USA
    Science 293:498-506. 2001
    ..Comparative genome hybridization with DNA arrays revealed strain differences in S. pneumoniae that could contribute to differences in virulence and antigenicity...
  100. pmc A modified sleeping beauty transposon system that can be used to model a wide variety of human cancers in mice
    Adam J Dupuy
    Department of Anatomy and Cell Biology, Center for Bioinformatics, Computational Biology and Biomedical Engineering, Roy J and Lucille A Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242, USA
    Cancer Res 69:8150-6. 2009
    ..These novel alleles provide additional tools for the SB system and provide some insight into how this mutagenesis system can be manipulated to model cancer in mice...

Research Grants143 found, 100 shown here

  1. Transposon Mediated Insertional Mutagenesis in Mice
    DAVID LARGAESPADA; Fiscal Year: 2003
    ..element called Sleeping Beauty (SB), which is a reconstructed member of the Tcl/mariner family of DNA transposable elements. We have demonstrated that efficient mobilization of SB transposons can be made to occur in the germline ..
  2. REGULATION OF AMPICILLIN RESISTANCE IN E FACEIUM
    Louis Rice; Fiscal Year: 2004
    ..They will also yield important new information on mechanisms of cell wall synthesis in E. faecium and other Gram-positive bacteria as well as on the mechanisms by which ampicillin resistance in E. faecium is regulated. ..
  3. RNAi, Histone Modification and the DDB1/CPSF-like Complex Rik1
    Robert A Martienssen; Fiscal Year: 2010
    ..Transposable elements and other repeats are common to all of these genomes, and may hold the key to epigenetic mechanisms of gene regulation and chromosome function. ..
  4. RNAi, Histone Modification and the DDB1/CPSF-like Complex Rik1
    Robert Martienssen; Fiscal Year: 2007
    ..Transposable elements and other repeats are common to all of these genomes, and may hold the key to epigenetic mechanisms of gene regulation and chromosome function. ..
  5. Selenium, Aminothiols, and Radiation
    Alan Diamond; Fiscal Year: 2004
    ..Collectively, these studies will provide new insight on how selenium protects cells from radiation-induced mutagenesis and may lead to novel approaches to increase the beneficial properties of this nutrient. ..
  6. Human DNA transposons: evolutionary history and genomic impact
    CEDRIC FESCHOTTE; Fiscal Year: 2010
    ..We will combine comparative and evolutionary sequence analyses to in vitro and in vivo assays to test whether these proteins (or other candidate identified in the course of this research) have retained their catalytic activities. ..
  7. Human DNA transposons: evolutionary history and genomic impact
    CEDRIC FESCHOTTE; Fiscal Year: 2007
    ..We will combine comparative and evolutionary sequence analyses to in vitro and in vivo assays to test whether these proteins (or other candidate identified in the course of this research) have retained their catalytic activities. ..
  8. Ampicillin resistance mechanisms in E. faecium
    Louis Rice; Fiscal Year: 2007
    ..abstract_text> ..
  9. Cell wall synthesis enzymes and beta-lactam resistance in Enterococcus faecium
    Louis B Rice; Fiscal Year: 2010
    ..This project aims to identify and characterize the complex and redundant mechanisms of ?-lactam resistance in an effort to better understand enterococcal physiology and promote identification of novel therapeutic targets. ..
  10. Genetic methods using the Tol2 transposable element
    Koichi Kawakami; Fiscal Year: 2006
    ..abstract_text> ..
  11. HOBO-LIKE ELEMENTS IN INSECTS
    DAVID O BROCHTA; Fiscal Year: 2004
    ..5) develop a functional anopheline hAT element-based gene vector system. These aims will be accomplished using genetic and molecular genetic methods. ..
  12. HOBO-LIKE ELEMENTS IN INSECTS
    DAVID O BROCHTA; Fiscal Year: 2007
    ..gambiae populations, 3) develop Herves into a mosquito gene vector. These aims will be accomplished by performing a combination of laboratory and field-based studies using molecular and population genetics tools. ..
  13. Genetic Manipulation of Insects
    DAVID O BROCHTA; Fiscal Year: 2004
    ..The meeting is an uncommon opportunity for graduate students and postdoctoral fellows to obtain a high level synopsis of the field of insect molecular genetics and to make important career-advancing interactions. ..
  14. Tn5 as an insect genomics tool
    DAVID O BROCHTA; Fiscal Year: 2002
    ..gambiae make this is a high risk project but one whose impact on the field of medical entomology and efforts to control the transmission of malaria will be enormous if it is successful. ..
  15. Gene drive potential of transposable elements in Anopheles gambiae.
    DAVID O BROCHTA; Fiscal Year: 2009
    ..gambiae will be known. This project involves the use of Animals but does not involve Human Subjects. ..
  16. HOBO-LIKE TRANSPOSABLE ELEMENTS IN INSECTS
    DAVID O BROCHTA; Fiscal Year: 1993
    ....
  17. HOBO-LIKE TRANSPOSABLE ELEMENTS IN INSECTS
    DAVID O BROCHTA; Fiscal Year: 1999
    ....
  18. Gene drive potential of transposable elements in Anopheles gambiae.
    DAVID A O apos BROCHTA; Fiscal Year: 2010
    ..gambiae will be known. This project involves the use of Animals but does not involve Human Subjects. ..
  19. Identification of Tetrahymena DNA Rearrangement Genes
    DOUGLAS LEE CHALKER; Fiscal Year: 2010
    ..abstract_text> ..
  20. MECHANISMS OF MYCOPLASMA-INDUCED ARTHRITIS
    Kevin Dybvig; Fiscal Year: 2002
    ..arthritidis by affecting interactions with host factors such as B and T cells and complement. Thus, the process of dissecting the function of MAV1 in virulence will be begun. ..
  21. Molecular Epidemiology of Community-Associated MRSA
    SANJAY SHUKLA; Fiscal Year: 2007
    ..We will determine the presence or absences of 40 different virulence factor genes in all 320 isolates listed in aim 2 and determine evolution of virulence factor genes in contemporary CA-MRSA. ..
  22. GENETIC RECOMBINATION IN MYCOPLASMAS
    Kevin Dybvig; Fiscal Year: 2004
    ..In aim 2, mutants with defects in putative DNA recombinases and V-I protein production will be examined to study the mechanisms of gene rearrangements and phenotypic switching. ..
  23. Tandemly Repetitive Proteins in Mycoplasmas
    Kevin Dybvig; Fiscal Year: 2007
    ..By understanding the role of repetitive proteins in the disease process, it is anticipated that improved measures to control the infections of these significant human pathogens will be developed. ..
  24. ANTIGENIC VARIATION IN MYCOPLASMAS
    Kevin Dybvig; Fiscal Year: 2004
    ....
  25. ROLE OF RHOMBOID HOMOLOGUES IN MYCOBACTERIA SIGNALING
    Moses Joloba; Fiscal Year: 2006
    ..This will expand our understanding of mycobacterial biology and may provide novel candidate vaccines and drug targets. ..
  26. RHEUMATOLOGY RESEARCH TRAINING PROGRAM
    MARY CROW; Fiscal Year: 2007
    ..Support is requested for 3 MD, MD-PhD, or PhD trainees who will gain the skills required to make important advances in the health of patients with rheumatic disease. ..
  27. RNA Polymerase III transcription in Leishmania major
    SANTIAGO MARTINEZ CALVILLO; Fiscal Year: 2007
    ..The tandem affinity purification (TAP-tag) method will be used to purify Pol III transcriptional complexes. The protein components in these complexes will be identified by mass-spectrometry analysis. ..
  28. Improved Brucella Vaccine Strains
    Thomas Ficht; Fiscal Year: 2009
    ..abstract_text> ..
  29. Interferon in Systemic Lupus Erythematosus
    MARY CROW; Fiscal Year: 2009
    ..Elucidation of this important pathway should lead to more targeted modulation of disease mediators in systemic autoimmune diseases. ..
  30. CELL TO CELL SIGNALING IN MYCOBACTERIA
    MOSES LUTAAKOME JOLOBA; Fiscal Year: 2010
    ..This will enrich our knowledge on mycobacterial biology and may reveal novel drug and vaccine targets. ..
  31. REQUIREMENTS FOR BACTERIAL COLONIZATION OF ANIMAL TISSUE
    Karen L Visick; Fiscal Year: 2010
    ....
  32. REQUIREMENTS FOR BACTERIAL COLONIZATION OF ANIMAL TISSUE
    KAREN VISICK; Fiscal Year: 2007
    ..parahaemolyticus and V. vulnificus. Studying this model may also lead to approaches that prevent, reduce or treat such infections. ..
  33. Interferon in Systemic Lupus Erythematosus
    Mary K Crow; Fiscal Year: 2010
    ..Elucidation of this important pathway should lead to more targeted modulation of disease mediators in systemic autoimmune diseases. ..
  34. CELL TO CELL SIGNALING IN MYCOBACTERIA
    Moses Joloba; Fiscal Year: 2007
    ..This will enrich our knowledge on mycobacterial biology and may reveal novel drug and vaccine targets. ..
  35. RNA Polymerase III transcription in Leishmania major
    SANTIAGO MARTINEZ CALVILLO; Fiscal Year: 2009
    ..The tandem affinity purification (TAP-tag) method will be used to purify Pol III transcriptional complexes. The protein components in these complexes will be identified by mass-spectrometry analysis. ..
  36. Identification of Rheumatic Disease Genes
    MARY CROW; Fiscal Year: 2004
    ..abstract_text> ..