dna adducts

Summary

Summary: Covalent adducts between chemical mutagens and DNA. Such couplings activate DNA repair processes and, unless repaired prior to DNA replication, may lead to nucleotide substitutions, deletions, and chromosome rearrangements. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)

Top Publications

  1. ncbi Molecular mechanisms of mammalian global genome nucleotide excision repair
    Ludovic C J Gillet
    Institute for Molecular Cancer Research, University of Zurich, Switzerland
    Chem Rev 106:253-76. 2006
  2. doi Aristolochic acid nephropathy: a worldwide problem
    Frédéric D Debelle
    Department of Nephrology, Dialysis and Renal Transplantation, Erasme Hospital, Brussels, Belgium
    Kidney Int 74:158-69. 2008
  3. ncbi Doxorubicin-DNA adducts induce a non-topoisomerase II-mediated form of cell death
    Lonnie P Swift
    Department of Biochemistry, La Trobe University, Victoria, Australia
    Cancer Res 66:4863-71. 2006
  4. ncbi Mechanisms of resistance to cisplatin
    M Kartalou
    Division of Bioengineering and Environmental Health and Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Mutat Res 478:23-43. 2001
  5. pmc Probing for DNA damage with β-hairpins: similarities in incision efficiencies of bulky DNA adducts by prokaryotic and human nucleotide excision repair systems in vitro
    Yang Liu
    Chemistry Department, New York University, New York, NY 10003, USA
    DNA Repair (Amst) 10:684-96. 2011
  6. ncbi AHR- and DNA-damage-mediated gene expression responses induced by benzo(a)pyrene in human cell lines
    Sarah L Hockley
    Section of Molecular Carcinogenesis, The Institute of Cancer Research, Surrey, UK
    Chem Res Toxicol 20:1797-810. 2007
  7. ncbi Tobacco smoke carcinogens, DNA damage and p53 mutations in smoking-associated cancers
    Gerd P Pfeifer
    Division of Biology, Beckman Research Institute of the City of Hope, Duarte, California, CA 91010, USA
    Oncogene 21:7435-51. 2002
  8. ncbi Oxidative damage to DNA: formation, measurement and biochemical features
    Jean Cadet
    Laboratoire Lesions des Acides Nucleiques, SCIB DRFMC and FRE 2600, CEA Grenoble, F 38054 Grenoble Cedex 9, France
    Mutat Res 531:5-23. 2003
  9. ncbi Human DNA polymerase kappa encircles DNA: implications for mismatch extension and lesion bypass
    Samer Lone
    Department of Structural and Chemical Biology, Mount Sinai School of Medicine, Box 1677, 1425 Madison Avenue, New York, NY 10029, USA
    Mol Cell 25:601-14. 2007
  10. ncbi Importance of chromium-DNA adducts in mutagenicity and toxicity of chromium(VI)
    Anatoly Zhitkovich
    Department of Pathology and Laboratory Medicine, Brown University, Box G E507, Providence, Rhode Island 02912, USA
    Chem Res Toxicol 18:3-11. 2005

Research Grants

Detail Information

Publications351 found, 100 shown here

  1. ncbi Molecular mechanisms of mammalian global genome nucleotide excision repair
    Ludovic C J Gillet
    Institute for Molecular Cancer Research, University of Zurich, Switzerland
    Chem Rev 106:253-76. 2006
  2. doi Aristolochic acid nephropathy: a worldwide problem
    Frédéric D Debelle
    Department of Nephrology, Dialysis and Renal Transplantation, Erasme Hospital, Brussels, Belgium
    Kidney Int 74:158-69. 2008
    ..Finally, despite the Food and Drug Administration's warnings concerning the safety of botanical remedies containing AA, these herbs are still sold via the Internet...
  3. ncbi Doxorubicin-DNA adducts induce a non-topoisomerase II-mediated form of cell death
    Lonnie P Swift
    Department of Biochemistry, La Trobe University, Victoria, Australia
    Cancer Res 66:4863-71. 2006
    ..Here, we show that doxorubicin-DNA adducts (formed by the coadministration of doxorubicin with non-toxic doses of formaldehyde-releasing prodrugs) induce ..
  4. ncbi Mechanisms of resistance to cisplatin
    M Kartalou
    Division of Bioengineering and Environmental Health and Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Mutat Res 478:23-43. 2001
    ..An improved understanding of the mechanisms of resistance operative in vivo has identified targets for intervention and may increase the utility of cisplatin for the treatment of cancer...
  5. pmc Probing for DNA damage with β-hairpins: similarities in incision efficiencies of bulky DNA adducts by prokaryotic and human nucleotide excision repair systems in vitro
    Yang Liu
    Chemistry Department, New York University, New York, NY 10003, USA
    DNA Repair (Amst) 10:684-96. 2011
    ..These observations suggest that, in addition to recognition involving the initial modified duplexes, downstream events involving UvrC can also play a role in distinguishing and processing different lesions in prokaryotic NER...
  6. ncbi AHR- and DNA-damage-mediated gene expression responses induced by benzo(a)pyrene in human cell lines
    Sarah L Hockley
    Section of Molecular Carcinogenesis, The Institute of Cancer Research, Surrey, UK
    Chem Res Toxicol 20:1797-810. 2007
    ..Promoter analysis identified candidate genes for direct transcriptional regulation by either AHR or p53. These analyses have further dissected and characterized the complex cellular response to BaP...
  7. ncbi Tobacco smoke carcinogens, DNA damage and p53 mutations in smoking-associated cancers
    Gerd P Pfeifer
    Division of Biology, Beckman Research Institute of the City of Hope, Duarte, California, CA 91010, USA
    Oncogene 21:7435-51. 2002
    ..The available data suggest that p53 mutations in lung cancers can be attributed to direct DNA damage from cigarette smoke carcinogens rather than to selection of pre-existing endogenous mutations...
  8. ncbi Oxidative damage to DNA: formation, measurement and biochemical features
    Jean Cadet
    Laboratoire Lesions des Acides Nucleiques, SCIB DRFMC and FRE 2600, CEA Grenoble, F 38054 Grenoble Cedex 9, France
    Mutat Res 531:5-23. 2003
    ..For this purpose modified oligonucleotides in which cyclopurine, and cyclopyrimidine nucleosides were site-specifically inserted were synthesized...
  9. ncbi Human DNA polymerase kappa encircles DNA: implications for mismatch extension and lesion bypass
    Samer Lone
    Department of Structural and Chemical Biology, Mount Sinai School of Medicine, Box 1677, 1425 Madison Avenue, New York, NY 10029, USA
    Mol Cell 25:601-14. 2007
    ....
  10. ncbi Importance of chromium-DNA adducts in mutagenicity and toxicity of chromium(VI)
    Anatoly Zhitkovich
    Department of Pathology and Laboratory Medicine, Brown University, Box G E507, Providence, Rhode Island 02912, USA
    Chem Res Toxicol 18:3-11. 2005
  11. ncbi Formation of PAH-DNA adducts after in vivo and vitro exposure of rats and lung cells to different commercial carbon blacks
    Paul J A Borm
    Particle Research, Institut für Umweltmedizinische Forschung IUF at the Heinrich Heine University, Dusseldorf, Germany
    Toxicol Appl Pharmacol 205:157-67. 2005
    The current study was designed to test the possible release and bioavailability of polycyclic aromatic hydrocarbons (PAHs) from a set of commercial carbon blacks (CBs) as well as the ability of these PAHs to form bulky DNA adducts.
  12. ncbi Carcinogenic polycyclic aromatic hydrocarbon-DNA adducts and mechanism of action
    William M Baird
    Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, Oregon 97331, USA
    Environ Mol Mutagen 45:106-14. 2005
    ..Thus, PAHs may also act through a promotional mechanism in addition to serving as tumor initiators. Previous studies on these mechanisms are described and summarized...
  13. ncbi Repair of O(6)-alkylguanine by alkyltransferases
    A E Pegg
    Departments of Cellular and Molecular Physiology and Pharmacology, Milton S Hershey Medical Center, Pennsylvania State University College of Medicine, P O Box 850, 500 University Drive, Hershey, PA, USA
    Mutat Res 462:83-100. 2000
    ..b>DNA adducts at the O(6)-position of guanine are a major factor in the carcinogenic, mutagenic, apoptopic and clastogenic ..
  14. ncbi DNA adducts from acetaldehyde: implications for alcohol-related carcinogenesis
    Philip J Brooks
    Section on Molecular Neurobiology, Laboratory of Neurogenetics, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, 5625 Fishers Lane, Room 3S32, MSC 9412, Bethesda, MD 20892 9412, USA
    Alcohol 35:187-93. 2005
    ..Inherited variation in the genes encoding the proteins involved in the repair of PdG and its secondary adducts may contribute to susceptibility to alcoholic beverage-related carcinogenesis...
  15. pmc Influence of flanking sequence context on the conformational flexibility of aminofluorene-modified dG adduct in dA mismatch DNA duplexes
    Nidhi Jain
    Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, RI 02881, USA
    Nucleic Acids Res 37:1628-37. 2009
    ....
  16. pmc Structures of 2-acetylaminofluorene modified DNA revisited: insight into conformational heterogeneity
    Satyakam Patnaik
    Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, Rhode Island 02881, USA
    Chem Res Toxicol 23:1650-2. 2010
    ..The S/B/W-conformational heterogeneities were found to be sensitive to the nature of the flanking DNA sequence contexts and pH...
  17. ncbi Probing the conformational heterogeneity of the acetylaminofluorene-modified 2'-deoxyguanosine and DNA by 19F NMR spectroscopy
    B P Cho
    Department of Biomedical Sciences, College of Pharmacy, University of Rhode Island, Kingston 02881, USA
    Biochemistry 38:7572-83. 1999
    ..et al. (1997) J. Am. Chem. Soc. 119, 5384-5389]. The exclusive stacked nature of the AAF adducts may provide insight into why AAF adducts are more mutagenic and prone to repair than the nonacetylated AF adducts...
  18. ncbi A single amino acid governs enhanced activity of DinB DNA polymerases on damaged templates
    Daniel F Jarosz
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nature 439:225-8. 2006
    ....
  19. ncbi Ochratoxin a causes DNA damage and cytogenetic effects but no DNA adducts in rats
    Angela Mally
    Department of Toxicology, University of Wurzburg, Germany
    Chem Res Toxicol 18:1253-61. 2005
    ..Oxidative DNA damage as a mechanism of OTA-dependent DNA damage is consistent with the absence of lipophilic DNA adducts as assessed by 32P-postlabeling analysis...
  20. pmc The vital role of polymerase ζ and REV1 in mutagenic, but not correct, DNA synthesis across benzo[a]pyrene-dG and recruitment of polymerase ζ by REV1 to replication-stalled site
    Keiji Hashimoto
    Department of Pharmacological Sciences, State University of New York, Stony Brook, New York 11794 8651, USA
    J Biol Chem 287:9613-22. 2012
    ..This indicates that a non-Y family polymerase(s) can insert a nucleotide opposite BPDE-dG, but the subsequent extension from miscoding termini depends on REV1-polζ in a RAD18-dependent manner...
  21. doi Processing of anthracycline-DNA adducts via DNA replication and interstrand crosslink repair pathways
    R A Bilardi
    Department of Biochemistry, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, Victoria 3086, Australia
    Biochem Pharmacol 83:1241-50. 2012
    ..of topoisomerase II, however many anthracyclines, including doxorubicin, are also capable of forming drug-DNA adducts. Anthracycline-DNA adducts present an unusual obstacle for cells as they are covalently attached to one DNA ..
  22. pmc Use of LC-MS/MS and stable isotopes to differentiate hydroxymethyl and methyl DNA adducts from formaldehyde and nitrosodimethylamine
    Kun Lu
    Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Chem Res Toxicol 25:664-75. 2012
    Formaldehyde is a known human and animal carcinogen that forms DNA adducts, and causes mutations. While there is widespread exposure to formaldehyde in the environment, formaldehyde is also an essential biochemical in all living cells...
  23. pmc Chemistry and structural biology of DNA damage and biological consequences
    Michael P Stone
    Department of Chemistry, Center in Molecular Toxicology, Vanderbilt Ingram Cancer Center, Vanderbilt University, Nashville, TN 37235, USA
    Chem Biodivers 8:1571-615. 2011
    ..The structural analysis of various DNA adducts reveals that conformational and chemical rearrangements and interconversions are a common theme...
  24. pmc Resistance of bulky DNA lesions to nucleotide excision repair can result from extensive aromatic lesion-base stacking interactions
    Dara A Reeves
    Department of Chemistry, Department of Biology, New York University, New York, NY 10003, USA
    Nucleic Acids Res 39:8752-64. 2011
    ....
  25. pmc Polycyclic aromatic hydrocarbons-aromatic DNA adducts in cord blood and behavior scores in New York city children
    Frederica P Perera
    Department of Environmental Health Sciences, Mailman School of Public Health, Columbia University, New York, New York 10032, USA
    Environ Health Perspect 119:1176-81. 2011
    Airborne polycyclic aromatic hydrocarbons (PAH) are widespread urban pollutants that can bind to DNA to form PAH-DNA adducts. Prenatal PAH exposure measured by personal monitoring has been linked to cognitive deficits in childhood in a ..
  26. pmc Site-specific radical formation in DNA induced by Cu(II)-H₂O₂ oxidizing system, using ESR, immuno-spin trapping, LC-MS, and MS/MS
    Suchandra Bhattacharjee
    Laboratory of Toxicology and Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 50:1536-45. 2011
    ..The DMPO adduct formed is initially EPR active but subsequently is oxidized to the stable nitrone, which can then be detected by IST and further characterized by MS and MS/MS...
  27. pmc Elevated levels of the pro-carcinogenic adduct, O(6)-methylguanine, in normal DNA from the cancer prone regions of the large bowel
    A C Povey
    Cancer Research Campaign Section of Genome Damage and Repair, Paterson Institute for Cancer Research, Manchester, M20 9BX, UK
    Gut 47:362-5. 2000
    ..The biological significance of this exposure is, as yet, unknown but in situ NOC formation is bacterially catalysed suggesting that NOC formation and potentially DNA alkylation will vary throughout the large bowel...
  28. ncbi XRCC1, XRCC3, XPD gene polymorphisms, smoking and (32)P-DNA adducts in a sample of healthy subjects
    G Matullo
    Dipartimento di Genetica, Biologia e Biochimica, Universita di Torino, 10126, Torino, ISI Foundation, Institute for Scientific Interchange, Villa Gualino, 10133, Torino, Italy
    Carcinogenesis 22:1437-45. 2001
    ..These data show that all the analysed polymorphisms could result in deficient DNA repair and suggest a need for further investigation into the possible interactions between these polymorphisms, smoking and other risk factors...
  29. doi The pyrrolobenzodiazepine dimer SJG-136 forms sequence-dependent intrastrand DNA cross-links and monoalkylated adducts in addition to interstrand cross-links
    Khondaker M Rahman
    Gene Targeted Drug Design Research Group, The School of Pharmacy, University of London, 29 39 Brunswick Square, London WC1N 1AX, UK
    J Am Chem Soc 131:13756-66. 2009
    ....
  30. ncbi Differential removal of DNA adducts derived from anti-diol epoxides of dibenzo[a,l]pyrene and benzo[a]pyrene in human cells
    Kristian Dreij
    The Institute of Environmental Medicine, Division of Biochemical Toxicology, Karolinska Institutet, Box 210, S 17177 Stockholm, Sweden
    Chem Res Toxicol 18:655-64. 2005
    ..of their ultimate carcinogenic diol epoxide (DE) intermediates, differences in structural features of the DNA adducts, and differences in DNA adduct recognition and the subsequent lesion removal by nucleotide excision repair (NER)..
  31. ncbi Standardization and validation of DNA adduct postlabelling methods: report of interlaboratory trials and production of recommended protocols
    D H Phillips
    Institute of Cancer Research, Haddow Laboratories, Sutton, Surrey, UK
    Mutagenesis 14:301-15. 1999
    ..was to devise and test improved protocols of the 32P-postlabelling assay for the detection of carcinogen-DNA adducts. The intention was to reverse the drift of different investigators using increasingly divergent experimental ..
  32. ncbi The 32P-postlabeling assay for DNA adducts
    David H Phillips
    Institute of Cancer Research, Brookes Lawley Building, Cotswold Road, Sutton, Surrey SM2 5NG, UK
    Nat Protoc 2:2772-81. 2007
    32P-postlabeling analysis is an ultrasensitive method for the detection and quantitation of carcinogen-DNA adducts. It consists of four principal steps: (i) enzymatic digestion of DNA to nucleoside 3'-monophosphates; (ii) enrichment of ..
  33. ncbi Validation of biomarkers for the study of environmental carcinogens: a review
    Valentina Gallo
    Department of Epidemiology and Public Health, Imperial College London, London, UK
    Biomarkers 13:505-34. 2008
    ..Our aim is to review published work on the validation of selected biomarkers: bulky DNA adducts, N-nitroso compounds, 1-hydroxypyrene, and oxidative damage to DNA...
  34. ncbi 5-Azacytidine induced methyltransferase-DNA adducts block DNA replication in vivo
    H Kenny Kuo
    Department of Biochemistry, Duke University, Durham, NC 27710, USA
    Cancer Res 67:8248-54. 2007
    ..These results imply that aza-C-induced MTase-DNA adducts block DNA replication in vivo. RecA-dependent X structures were also observed after aza-C treatment...
  35. ncbi Human biomonitoring: state of the art
    Jurgen Angerer
    Institute and Outpatient Clinic of Occupational, Social and Environmental Medicine, University of Erlangen Nuremberg, Schillerstrasse 25 29, 91054 Erlangen, Germany
    Int J Hyg Environ Health 210:201-28. 2007
    ..Protein adducts, especially Hb-adducts, as surrogates of DNA adducts measuring exposure as well as biochemical effect very specifically and sensitively are a still better means to ..
  36. pmc Site-specific incorporation of N-(deoxyguanosin-8-yl)-2-acetylaminofluorene (dG-AAF) into oligonucleotides using modified 'ultra-mild' DNA synthesis
    Ludovic C J Gillet
    Institute for Molecular Cancer Research, University of Zürich August Forel Strasse 7, 8008 Zurich, Switzerland
    Nucleic Acids Res 33:1961-9. 2005
    ..Our synthetic approach should facilitate comprehensive studies of the mechanisms of repair and mutagenesis induced by dG-AAF adducts in DNA and should be of general use for the incorporation of base-labile functionalities into DNA...
  37. pmc The sequence dependence of human nucleotide excision repair efficiencies of benzo[a]pyrene-derived DNA lesions: insights into the structural factors that favor dual incisions
    Konstantin Kropachev
    Department of Chemistry, New York University, New York, NY 10003, USA
    J Mol Biol 386:1193-203. 2009
    Nucleotide excision repair (NER) is a vital cellular defense system against carcinogen-DNA adducts, which, if not repaired, can initiate cancer development...
  38. pmc Intra-hepatic arterial administration with miriplatin suspended in an oily lymphographic agent inhibits the growth of tumors implanted in rat livers by inducing platinum-DNA adducts to form and massive apoptosis
    Mitsuharu Hanada
    Pharmacology Research Laboratories, Dainippon Sumitomo Pharma Co, Ltd, Osaka, Japan
    Cancer Chemother Pharmacol 64:473-83. 2009
    ..We clarified the usefulness of miriplatin as an agent for transarterial chemoembolization...
  39. ncbi Arsenite and its biomethylated metabolites interfere with the formation and repair of stable BPDE-induced DNA adducts in human cells and impair XPAzf and Fpg
    Tanja Schwerdtle
    Institute of Food Chemistry and Toxicology, University of Karlsruhe, Postfach 6980, D 76128, Karlsruhe, Germany
    DNA Repair (Amst) 2:1449-63. 2003
    ..Altogether the results suggest that besides arsenite, especially the trivalent methylated metabolites may contribute to diminished NER at low concentrations...
  40. ncbi Adverse reproductive outcomes from exposure to environmental mutagens
    R J Sram
    Laboratory of Genetic Ecotoxicology, Regional Institute of Hygiene of Central Bohemia, c o Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20, Prague, Czech Republic
    Mutat Res 428:203-15. 1999
    ..Genotoxicity of urban air particles <10 microm (PM10) in in vitro system was determined by the analysis of DNA adducts. The highest DNA binding activity was observed in aromatic fraction, identifying DNA adducts of carcinogenic ..
  41. pmc Acrolein: sources, metabolism, and biomolecular interactions relevant to human health and disease
    Jan F Stevens
    Department of Pharmaceutical Sciences, Oregon State University, Corvallis, OR, USA
    Mol Nutr Food Res 52:7-25. 2008
    ..Acrolein adduction disrupts the function of these biomacromolecules which may result in mutations, altered gene transcription, and modulation of apoptosis...
  42. ncbi AlkB reverses etheno DNA lesions caused by lipid oxidation in vitro and in vivo
    James C Delaney
    Biological Engineering Division, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nat Struct Mol Biol 12:855-60. 2005
    ..These reactions show a previously unrecognized chemical versatility of AlkB. In mammals, the corresponding AlkB homologs may defend against aging, cancer and oxidative stress...
  43. ncbi Metabolic activation of polycyclic and heterocyclic aromatic hydrocarbons and DNA damage: a review
    Weiling Xue
    Department of Environmental Health, University of Cincinnati Medical Center, 3223 Eden Avenue, Cincinnati, OH 45267 0056, USA
    Toxicol Appl Pharmacol 206:73-93. 2005
    ..The review emphasizes the chemical/biochemical reactions involved in the metabolic processes and the chemical structures of metabolites and DNA adducts.
  44. ncbi Safrole-DNA adducts in human peripheral blood--an association with areca quid chewing and CYP2E1 polymorphisms
    Tsung Yun Liu
    Institute of Pharmacology, National Yang Ming University, Taipei, Taiwan, ROC
    Mutat Res 559:59-66. 2004
    It has been recently demonstrated that safrole (4-allyl-1,2-methylenedioxybenzene)-DNA adducts are present in oral cancer tissue from patients who have chewed areca quid (AQ) containing high concentration of safrole...
  45. ncbi Interactive effects of nrf2 genotype and oltipraz on benzo[a]pyrene-DNA adducts and tumor yield in mice
    Minerva Ramos-Gomez
    Department of Environmental Health Sciences, Bloomberg School of Public Health, The Johns Hopkins University, Baltimore, MD 21202, USA
    Carcinogenesis 24:461-7. 2003
    ..To evaluate the role of nrf2 genotype on B[a]P disposition, levels of B[a]P-DNA adducts were measured as tetrols released from DNA isolated from target (forestomach) and non-target tissues (liver) of ..
  46. ncbi Hierarchy of DNA damage recognition in Escherichia coli nucleotide excision repair
    Y Zou
    Sealy Center for Molecular Science and Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, Texas 77555, USA
    Biochemistry 40:2923-31. 2001
    ..These data suggest that E. coli NER features a dynamic two-stage recognition mechanism...
  47. ncbi Modulation of gene expression and DNA adduct formation in HepG2 cells by polycyclic aromatic hydrocarbons with different carcinogenic potencies
    Yvonne C M Staal
    Department of Health Risk Analysis and Toxicology, Maastricht University, The Netherlands
    Carcinogenesis 27:646-55. 2006
    ..Our data indicate that discrimination of high and low carcinogenic PAHs by gene expression profiling is feasible. Also, the carcinogenic PAHs induce several pathways that were not affected by the least carcinogenic PAHs...
  48. pmc Endogenous versus exogenous DNA adducts: their role in carcinogenesis, epidemiology, and risk assessment
    James A Swenberg
    Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Toxicol Sci 120:S130-45. 2011
    ..known human carcinogens": formaldehyde, vinyl chloride (VC), and ethylene oxide (EO), each of which forms DNA adducts identical to endogenous DNA adducts in all individuals...
  49. ncbi Resveratrol, a natural aryl hydrocarbon receptor antagonist, protects lung from DNA damage and apoptosis caused by benzo[a]pyrene
    Ariel Revel
    Division of Reproductive Sciences, Department of Obstetrics and Gynecology, University of Toronto, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 21X5, Canada
    J Appl Toxicol 23:255-61. 2003
    ..in the aetiology of lung cancer through the induction of benzo[a]pyrene diol epoxidation (BPDE) and BPDE-DNA adducts. Because BaP metabolization requires cytochrome P-450 1A1 (CYP1A1) induction through activation of the AhR, we ..
  50. ncbi Dynamic conformational heterogeneities of carcinogen-DNA adducts and their mutagenic relevance
    Bongsup P Cho
    Department of Biomedical and Pharmaceutical Sciences, University of Rhode Island, Kingston, RI 02882, USA
    J Environ Sci Health C Environ Carcinog Ecotoxicol Rev 22:57-90. 2004
    ..Results from such experiments should provide an accurate account of how a single chemically homogenous adduct gives rise to complex multiple mutations, the earliest step in the induction of cancer...
  51. ncbi Effect of DNA repair gene polymorphisms on BPDE-DNA adducts in human lymphocytes
    Roberta Pastorelli
    Laboratory of Molecular Toxicology, Department of Environmental Health Sciences, Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy
    Int J Cancer 100:9-13. 2002
    ..BPDE-DNA adducts were analyzed by high-resolution gas chromatography-negative ion chemical ionization-mass spectrometry...
  52. ncbi PAH-DNA adducts in environmentally exposed population in relation to metabolic and DNA repair gene polymorphisms
    Blanka Binkova
    Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine AS CR and Health Institute of Central Bohemia, Videnska 1083, 14220 Prague, Czech Republic
    Mutat Res 620:49-61. 2007
    ..This study investigates relationships between exposure to PAHs, polymorphisms of these genes and DNA adducts in group of occupationally exposed policemen (EXP, N=53, males, aged 22-50 years) working outdoors in the ..
  53. ncbi Mutagenic events in Escherichia coli and mammalian cells generated in response to acetylaminofluorene-derived DNA adducts positioned in the Nar I restriction enzyme site
    Xingzhi Tan
    Laboratory of Chemical Biology, Department of Pharmacological Sciences, State University of New York at Stony Brook, Stony Brook, New York 11794 8651, USA
    Biochemistry 41:14255-62. 2002
    ..3-21%, the higher value at G(2). We conclude from this study that the mutation potential of dG-AAF and dG-AF depends on the structure of the adduct, the sequence context of the lesion, and the host cell used for the experiment...
  54. ncbi DNA interstrand crosslinks: natural and drug-induced DNA adducts that induce unique cellular responses
    ORLANDO D SCHARER
    Institute of Molecular Cancer Research, University of Zurich, August Forel Strasse 7, 8008 Zurich, Switzerland
    Chembiochem 6:27-32. 2005
  55. ncbi The genotoxic effect of carcinogenic PAHs, their artificial and environmental mixtures (EOM) on human diploid lung fibroblasts
    Blanka Binkova
    Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic
    Mutat Res 547:109-21. 2004
    ..We determined DNA adducts by (32)P -postlabelling and the expression of p53 and p21(WAF1) proteins by Western blotting...
  56. ncbi Thermodynamic and structural factors in the removal of bulky DNA adducts by the nucleotide excision repair machinery
    Nicholas E Geacintov
    Chemistry Department, New York University, New York, NY 10003 5180, USA
    Biopolymers 65:202-10. 2002
    ..These structural effects correlate with the differential NER susceptibilities and stem from the intrinsically distinct shapes of the fjord and bay region PAH diol epoxide-N(6)-adenine adducts...
  57. pmc Molecular evidence of an interaction between prenatal environmental exposures and birth outcomes in a multiethnic population
    Frederica P Perera
    Columbia Center for Children s Environmental Health, Mailman School of Public Health, Columbia University, 60 Haven Avenue B 109, New York, NY 10032, USA
    Environ Health Perspect 112:626-30. 2004
    ..ETS, using questionnaire data and plasma cotinine as a biomarker of exposure, and environmental PAHs using BaP-DNA adducts as a molecular dosimeter...
  58. ncbi Benzo[a]pyrene-7,8-quinone-3'-mononucleotide adduct standards for 32P postlabeling analyses: detection of benzo[a]pyrene-7,8-quinone-calf thymus DNA adducts
    Narayanan Balu
    Environmental Carcinogenesis Division, Office of Research and Development, U S Environmental Protection Agency, Research Triangle Park, NC 27711, USA
    Anal Biochem 355:213-23. 2006
    ..Chem. Res. Toxicol. 17 (2004) 827-838). However, the identification of BPQ-DNA adducts by 32P postlabeling methods from in vitro and in vivo exposures required 3'-monophosphate derivatives of BPQ-dG,..
  59. ncbi Lipid peroxidation-induced etheno-DNA adducts in the liver of patients with the genetic metal storage disorders Wilson's disease and primary hemochromatosis
    J Nair
    Division of Toxicology and Cancer Risk Factors, Deutsches Krebsforschungszentrum, Heidelberg, Germany
    Cancer Epidemiol Biomarkers Prev 7:435-40. 1998
    ..Thus, the etheno adducts are implicated as initiating DNA damage in copper/iron-induced carcinogenesis in humans and should also be explored as biomarkers in disease progression and prevention trials...
  60. ncbi Bulky DNA adducts, 4-aminobiphenyl-haemoglobin adducts and diet in the European Prospective Investigation into Cancer and Nutrition (EPIC) prospective study
    Marco Peluso
    Cancer Risk Factor Branch, Analytical and Biomolecular Cytology Unit, CSPO Scientific Institute of Tuscany, Florence, Italy
    Br J Nutr 100:489-95. 2008
    ..and Nutrition (EPIC) cohort the association between dietary items (food groups and nutrients) and aromatic DNA adducts and 4-aminobiphenyl-Hb adducts...
  61. ncbi The mismatch-repair protein hMSH2 binds selectively to DNA adducts of the anticancer drug cisplatin
    J A Mello
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Chem Biol 3:579-89. 1996
    ..cis-DDP or cisplatin) exerts its cytotoxic effects through the formation of covalent DNA adducts. A family of proteins possessing a common HMG box motif that binds specifically to cisplatin DNA adducts has ..
  62. pmc Conformational and thermodynamic properties modulate the nucleotide excision repair of 2-aminofluorene and 2-acetylaminofluorene dG adducts in the NarI sequence
    Vipin Jain
    Department of Biomedical and Pharmaceutical Sciences, University of Rhode Island, Kingston, RI 02881, USA
    Nucleic Acids Res 40:3939-51. 2012
    ..The present results provide valuable conformational insight into the sequence-dependent UvrABC incisions of the bulky aminofluorene DNA adducts.
  63. doi Phenylalanine 171 is a molecular brake for translesion synthesis across benzo[a]pyrene-guanine adducts by human DNA polymerase kappa
    Akira Sassa
    Division of Genetics and Mutagenesis, National Institute of Health Sciences, 1 18 1 Kamiyoga, Setagaya Ku, Tokyo 158 8501, Japan
    Mutat Res 718:10-7. 2011
    ..Human polymerase kappa (Pol κ) bypasses benzo[a]pyrene diolepoxide-N(2)-deoxyguanine (BPDE-N(2)-dG) DNA adducts in an almost error-free manner...
  64. pmc cis-Diammine(pyridine)chloroplatinum(II), a monofunctional platinum(II) antitumor agent: Uptake, structure, function, and prospects
    Katherine S Lovejoy
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 105:8902-7. 2008
    ..cDPCP-DNA adducts are removed by the nucleotide excision repair apparatus, albeit much less efficiently than bifunctional ..
  65. ncbi Morinda citrifolia (noni) reduces cancer risk in current smokers by decreasing aromatic DNA adducts
    Mian Ying Wang
    College of Medicine at Rockford, University of Illinois Chicago, Rockford, Illinois 61107, USA
    Nutr Cancer 61:634-9. 2009
    Quantitative determination of aromatic DNA adducts in peripheral blood lymphocytes (PBLs) of current smokers is an useful surrogate biomarker for the evaluation of environmental carcinogen exposure or chemopreventive intervention...
  66. doi Aromatic DNA adducts and risk of gastrointestinal cancers: a case-cohort study within the EPIC-Spain
    Antonio Agudo
    Unit of Nutrition, Environment and Cancer, Cancer Epidemiology Research Program, Catalan Institute of Oncology ICO, Av Gran Vía 199 203, L Hospitalet de Llobregat, Spain
    Cancer Epidemiol Biomarkers Prev 21:685-92. 2012
    ..Activated metabolites of these compounds may bind to DNA forming bulky adducts...
  67. pmc Repair of laser-localized DNA interstrand cross-links in G1 phase mammalian cells
    Parameswary A Muniandy
    Laboratory of Molecular Gerontology, NIA, National Institutes of Health, Baltimore, Maryland 21224, USA
    J Biol Chem 284:27908-17. 2009
    ..DDB2 accumulation at sites of cross-link repair is a marker for the start of the second repair cycle...
  68. doi DNA adducts and oxidative DNA damage induced by organic extracts from PM2.5 in an acellular assay
    Jan Topinka
    Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague 4, Czech Republic
    Toxicol Lett 202:186-92. 2011
    ..8-oxodG, levels) induced by EOMs in an acellular assay of calf thymus DNA coupled with ³²P-postlabeling (DNA adducts) and ELISA (8-oxodG) in the presence and absence of microsomal S9 fraction were employed...
  69. pmc Mutagenesis and repair induced by the DNA advanced glycation end product N2-1-(carboxyethyl)-2'-deoxyguanosine in human cells
    Daniel Tamae
    Division of Molecular Medicine, Beckman Research Institute of the City of Hope, 1500 East Duarte Road, Duarte, California 91010, United States
    Biochemistry 50:2321-9. 2011
    ..0001). This suggested the involvement of an NER-dependent mutagenic process in response to critical levels of DNA damage, possibly mediated by error-prone Y-family polymerases...
  70. doi Upregulation of clusterin in prostate and DNA damage in spermatozoa from bisphenol A-treated rats and formation of DNA adducts in cultured human prostatic cells
    Silvio De Flora
    Department of Health Sciences, University of Genoa, Genoa, Italy
    Toxicol Sci 122:45-51. 2011
    ..We previously demonstrated that administration of BPA to female mice results in the formation of DNA adducts and proteome alterations in the mammary tissue...
  71. ncbi Hijacking of the human alkyl-N-purine-DNA glycosylase by 3,N4-ethenocytosine, a lipid peroxidation-induced DNA adduct
    Laurent Gros
    Groupe Réparation de l ADN, CNRS Unité Mixte de Recherche 8113 LBPA ENS Cachan, Institut Gustave Roussy, 39, rue Camille Desmoulins, 94805 Villejuif Cedex, France
    J Biol Chem 279:17723-30. 2004
    ..The proposed model of ANPG-mediated genotoxicity of epsilonC provides a new insight in the molecular basis of lipid peroxidation-induced cell death and genome instability in cancer...
  72. ncbi Sequence distribution of acetaldehyde-derived N2-ethyl-dG adducts along duplex DNA
    Brock Matter
    University of Minnesota Cancer Center and Department of Medicinal Chemistry, Minneapolis 55455, USA
    Chem Res Toxicol 20:1379-87. 2007
    ..These results are in contrast with sequence-selective formation of other tobacco carcinogen-DNA adducts along K-ras- and p53-derived duplexes and the preferential modification of endogenously methylated CG ..
  73. ncbi NMR evidence for syn-anti interconversion of a trans opened (10R)-dA adduct of benzo[a]pyrene (7S,8R)-diol (9R,10S)-epoxide in a DNA duplex
    D E Volk
    Sealy Center for Structural Biology and Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, Texas 77555 1157, USA
    Biochemistry 39:14040-53. 2000
    ..The existence of conformational flexibility around adducts may be related to the occurrence of multiple mutagenic outcomes resulting from a single DE adduct...
  74. pmc DNA adducts of aristolochic acid II: total synthesis and site-specific mutagenesis studies in mammalian cells
    Sivaprasad Attaluri
    Department of Pharmacological Sciences, Stony Brook University, Stony Brook, NY 11794 3400, USA
    Nucleic Acids Res 38:339-52. 2010
    ..AA-I is largely responsible for the nephrotoxicity while both AA-I and AA-II are genotoxic. DNA adducts derived from AA-I and AA-II have been isolated from renal tissues of patients suffering from AAN...
  75. pmc Case-control study of an acute aflatoxicosis outbreak, Kenya, 2004
    Eduardo Azziz-Baumgartner
    National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, Georgia 30341 3717, USA
    Environ Health Perspect 113:1779-83. 2005
    ..We conducted a case-control study to identify risk factors for contamination of implicated maize and, for the first time, quantitated biomarkers associated with acute aflatoxicosis...
  76. ncbi Further arguments in favour of direct covalent binding of Ochratoxin A (OTA) after metabolic biotransformation
    A Pfohl-Leszkowicz
    INP ENSAT, UMR CNRS INPT UPS 5503, Department Bioprocess and Microbial Engineering, 1 Avenue Agrobiopole, Auzeville Tolosane, France
    Food Addit Contam 22:75-87. 2005
    ..Several OTA metabolites have been isolated from tissues or cells treated by OTA. The open ring lactone (OP-OTA) and quinone OTA (OTQ) are genotoxic...
  77. ncbi Evidence for covalent DNA adduction by ochratoxin A following chronic exposure to rat and subacute exposure to pig
    Virginie Faucet
    Department Toxicology and Food Safety, Lab Genie Chimique, Ecole Nationale Superieure Agronomique de Toulouse, UMR CNRS 5503, Agrobiopole BP 107, F31326 Castanet, Tolosan Cedex, France
    Chem Res Toxicol 17:1289-96. 2004
    ..genotoxicity of OTA and its ability to react directly with DNA upon oxidative bioactivation to yield covalent DNA adducts. To characterize DNA adduction by OTA, the present study utilizes the photooxidative properties of the toxin to ..
  78. ncbi Chloroethylnitrosourea-derived ethano cytosine and adenine adducts are substrates for Escherichia coli glycosylases excising analogous etheno adducts
    Anton B Guliaev
    Life Sciences Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA 94720, USA
    DNA Repair (Amst) 3:1311-21. 2004
    Exocyclic ethano DNA adducts are saturated etheno ring derivatives formed mainly by therapeutic chloroethylnitrosoureas (CNUs), which are also mutagenic and carcinogenic...
  79. ncbi Formation of diastereomeric benzo[a]pyrene diol epoxide-guanine adducts in p53 gene-derived DNA sequences
    Brock Matter
    Department of Medicinal Chemistry, University of Minnesota Cancer Center, Minneapolis, Minnesota 55455, USA
    Chem Res Toxicol 17:731-41. 2004
    ..The targeted formation of N(2)-BPDE-dG at (Me)CG dinucleotides within the p53 gene is consistent with the high prevalence of G --> T transversions at these sites in smoking-induced lung cancer...
  80. ncbi Polycyclic aromatic hydrocarbon-DNA adducts in human sperm as a marker of DNA damage and infertility
    Laura Gaspari
    Molecular and Genetic Epidemiology Unit, Padiglione Marangoni, Ospedale Policlinico, IRCCS University of Milano, Via F Sforza 35, 20122, Milano, Italy
    Mutat Res 535:155-60. 2003
    ..In order to assess whether polycyclic aromatic hydrocarbon (PAH)-DNA adducts are an early marker of sperm genotoxicity and infertility, we studied 205 men consecutively recruited from 1 ..
  81. ncbi Highly sensitive chemiluminescence immunoassay for benzo[a]pyrene-DNA adducts: validation by comparison with other methods, and use in human biomonitoring
    Rao L Divi
    National Cancer Institute, NIH, Bethesda, MD 20892 4255, USA
    Carcinogenesis 23:2043-9. 2002
    ..pyrene (BPDE) has been developed and validated to study the formation of polycyclic aromatic hydrocarbon (PAH)-DNA adducts in human tissues...
  82. pmc Antitumour 2-(4-aminophenyl)benzothiazoles generate DNA adducts in sensitive tumour cells in vitro and in vivo
    C O Leong
    School of Pharmaceutical Sciences, University of Nottingham, UK
    Br J Cancer 88:470-7. 2003
    ..In vitro, 2-(4-aminophenyl)benzothiazole-generated DNA adducts form in sensitive tumour cells only...
  83. doi DNA adducts and cancer risk in prospective studies: a pooled analysis and a meta-analysis
    Fabrizio Veglia
    Life Sciences and Epidemiology Unit, ISI Foundation, Torino 10133, Italy
    Carcinogenesis 29:932-6. 2008
    Bulky DNA adducts are biomarkers of exposure to aromatic compounds and of the ability of the individual to metabolically activate carcinogens and to repair DNA damage. Their ability to predict cancer onset is uncertain...
  84. ncbi Protective effects of vanadium against DMH-induced genotoxicity and carcinogenesis in rat colon: removal of O(6)-methylguanine DNA adducts, p53 expression, inducible nitric oxide synthase downregulation and apoptotic induction
    Shaonly Samanta
    Division of Biochemistry, Department of Pharmaceutical Technology, Jadavpur University, Calcutta, India
    Mutat Res 650:123-31. 2008
    ..These studies cumulatively provide strong evidence for the inhibitory actions of vanadium against DMH-induced genotoxicity and carcinogenesis in rat colon...
  85. pmc Seasonal variations in the levels of PAH-DNA adducts in young adults living in Mexico City
    W A García-Suástegui
    Departamento de Medicina Genómica y Toxicología Ambiental, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, A P 70 228, Ciudad Universitaria 04510, Mexico City, Mexico
    Mutagenesis 26:385-91. 2011
    ..of some PAHs produces reactive metabolites that bind to DNA, and the presence of elevated levels of PAH-DNA adducts in tissues such as blood lymphocytes represents an elevated risk for the development of cancer...
  86. pmc Grilled meat consumption and PhIP-DNA adducts in prostate carcinogenesis
    Deliang Tang
    Environmental Health Science, Columbia University Mailman School of Public Health, 630 W 168th Street, P and S 19 407, New York, NY, USA
    Cancer Epidemiol Biomarkers Prev 16:803-8. 2007
    ..PhIP derives its carcinogenic potential through the formation of PhIP-DNA adducts. The purpose of this study was to examine whether self-reported consumption and preparation doneness of grilled ..
  87. pmc Intercalative conformations of the 14R (+)- and 14S (-)-trans-anti-DB[a,l]P-N⁶-dA adducts: molecular modeling and MD simulations
    Yuqin Cai
    Department of Biology, New York University, New York, NY 10003, United States
    Chem Res Toxicol 24:522-31. 2011
    ..The conformational properties of DB[a,l]P-derived DNA adducts responsible for its extraordinary carcinogenicity are hence of great interest...
  88. pmc The human DNA repair factor XPC-HR23B distinguishes stereoisomeric benzo[a]pyrenyl-DNA lesions
    Vincent Mocquet
    Chemistry Department, Institut de Genetique et de Biologie Moleculaire et Cellulaire, CNRS INSERM ULP, Strasbourg, France
    EMBO J 26:2923-32. 2007
    ....
  89. pmc Distribution of DNA adducts caused by inhaled formaldehyde is consistent with induction of nasal carcinoma but not leukemia
    Kun Lu
    Department of Environmental Sciences and Engineering, The University of North Carolina at Chapel Hill, North Carolina 27599, USA
    Toxicol Sci 116:441-51. 2010
    ..Formation of DNA adducts is a key event in initiating carcinogenesis...
  90. pmc Molecular dosimetry of N2-hydroxymethyl-dG DNA adducts in rats exposed to formaldehyde
    Kun Lu
    Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill, North Carolina 27599, United States
    Chem Res Toxicol 24:159-61. 2011
    ..Our data clearly demonstrated that exogenous formaldehyde DNA adducts form in a highly nonlinear fashion, with a 21...
  91. doi Trapping of human DNA topoisomerase I by DNA structures mimicking intermediates of DNA repair
    Natalia Lebedeva
    Institute of Chemical Biology and Fundamental Medicine, Prospect Lavrentieva 8, 630090, Novosibirsk, Russia
    IUBMB Life 60:130-4. 2008
    ..Top1-DNA covalent adducts formed in cells exposed to DNA damaging agents can promote genetic instability...
  92. doi Depurinating estrogen-DNA adducts in the etiology and prevention of breast and other human cancers
    Ercole L Cavalieri
    Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, USA
    Future Oncol 6:75-91. 2010
    Experiments on estrogen metabolism, formation of DNA adducts, mutagenicity, cell transformation and carcinogenicity have led to and supported the hypothesis that the reaction of specific estrogen metabolites, mostly the electrophilic ..
  93. ncbi Kinetic analysis of the stepwise formation of a long-range DNA interstrand cross-link by a dinuclear platinum antitumor complex: evidence for aquated intermediates and formation of both kinetically and thermodynamically controlled conformers
    J W Cox
    Department of Chemistry, Virginia Commonwealth University, Richmond, Virginia 23284-2006, USA
    J Am Chem Soc 123:1316-26. 2001
    ..The potential significance of these findings to the antitumor activity of dinuclear platinum complexes is discussed...
  94. pmc Formation and accumulation of pyridyloxobutyl DNA adducts in F344 rats chronically treated with 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone and enantiomers of its metabolite, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol
    Yanbin Lao
    Department of Medicinal Chemistry, and the Cancer Center, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Chem Res Toxicol 20:235-45. 2007
    ..in the lung, the reconversion to NNK, and then the metabolic activation of NNK to pyridyloxobutyl (POB)-DNA adducts. We tested this hypothesis by treating male F344 rats with 10 ppm of NNK, (R)-NNAL, or (S)-NNAL in drinking ..
  95. pmc Estrogen metabolism and formation of estrogen-DNA adducts in estradiol-treated MCF-10F cells. The effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin induction and catechol-O-methyltransferase inhibition
    Fang Lu
    Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, 986805 Nebraska Medical Center, Omaha, NE 68198 6805, USA
    J Steroid Biochem Mol Biol 105:150-8. 2007
    ..Estrogen metabolites and depurinating DNA adducts in culture medium were analyzed by HPLC with electrochemical detection...
  96. ncbi Conformation, recognition by high mobility group domain proteins, and nucleotide excision repair of DNA intrastrand cross-links of novel antitumor trinuclear platinum complex BBR3464
    J Zehnulova
    Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska 135, CZ-61265 Brno, Czech Republic
    J Biol Chem 276:22191-9. 2001
    ..Hence, polynuclear platinum compounds apparently represent a novel class of platinum anticancer drugs acting by a different mechanism than cisplatin and its analogues...
  97. ncbi Leukocyte polycyclic aromatic hydrocarbon-DNA adduct formation and colorectal adenoma
    Marc J Gunter
    Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Division of Health and Human Services, Bethesda, MD 20852, USA
    Carcinogenesis 28:1426-9. 2007
    ..Leukocyte PAH-DNA adducts were measured by a sensitive chemiluminescence immunoassay using an antiserum elicited against DNA modified ..
  98. ncbi Glutathione conjugation and DNA adduct formation of dibenzo[a,l]pyrene and benzo[a]pyrene diol epoxides in V79 cells stably expressing different human glutathione transferases
    Kathrin Sundberg
    Institute of Environmental Medicine, Division of Biochemical Toxicology and Experimental Carcinogenesis, Karolinska Institutet, Box 210, S 17177 Stockholm, Sweden
    Chem Res Toxicol 15:170-9. 2002
    ....
  99. ncbi Experimental and theoretical studies on the pharmacodynamics of cisplatin in jurkat cells
    Kirk A Tacka
    Department of Chemistry, Syracuse University, 111 College Place, CST Rm1 014, Syracuse, New York 13244 4100, USA
    Chem Res Toxicol 17:1434-44. 2004
    ..The number of platinum-DNA adducts is measured immediately following exposure to drug...
  100. ncbi Evaluation of 4-aminobiphenyl-DNA adducts in human breast cancer: the influence of tobacco smoke
    Beatrice Faraglia
    Department of Environmental Health Sciences, Mailman School of Public Health, Columbia University, 701 West 168th Street, New York, NY 10032, USA
    Carcinogenesis 24:719-25. 2003
    ..In this study, we investigated the role of 4-ABP in the etiology of human breast cancer by measuring 4-ABP-DNA adducts using a monoclonal antibody based immunoperoxidase method that had been validated by comparison with gas ..
  101. ncbi Quantitative analysis of translesion DNA synthesis across a benzo[a]pyrene-guanine adduct in mammalian cells: the role of DNA polymerase kappa
    Sharon Avkin
    Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
    J Biol Chem 279:53298-305. 2004
    ..These results indicate that BP-G is bypassed in mammalian cells with relatively high efficiency and that polkappa bypasses BP-G in vivo with higher efficiency and higher accuracy than other DNA polymerases...

Research Grants98

  1. MODULATING BASE EXCISION REPAIR IN TUMOR DRUG RESISTANCE
    Stanton Gerson; Fiscal Year: 2007
    ..such as temozolomide (TMZ) form O6-methylguanine (O6/mG), 7-methylguanine (N7mG), and 3-methyladenine (N3mA) DNA adducts, repaired by at least two mechanisms and a third mechanism that mediates cell death...
  2. Isomeric HRT estrogen-DNA adducts: Structure and Repair
    Nicholas Geacintov; Fiscal Year: 2009
    ..is the most significant metabolite among all three equine estrogens, and that it forms unusual cyclic, stable DNA adducts in vitro, in rat mammary tissue model systems, and in human breast tissue...
  3. ALTERED METABOLISM AND CARCINOGENESIS BY DES
    CORAL LAMARTINIERE; Fiscal Year: 1990
    ..chromatography (HPLC) and spectrophotometry will be used for measuring metabolic end products and aflatoxin-DNA adducts. These experiments will provide us with kinetics of carcinogen-DNA adducts that lead to promutagenesis...
  4. DNA Repair Capacity and Risk of Pre-malignant Lesion in Lung Epithelium
    Hua Zhao; Fiscal Year: 2009
    ..ROS-caused base damages are mainly repaired by base excision repair (BER) and nucleotide excision repair (NER);DNA adducts caused by MPO- related carcinogen activation are repaired by NER...
  5. H pylori induced oxidative DNA damage
    Duane Smoot; Fiscal Year: 2001
    ..DNA damage in gastric epithelial cell lines; and elucidate the spectrum and repair course of oxidant related DNA adducts formed after exposure to H. pylori (live bacteria vs...
  6. Molecular mechanisms of DNA damage by PAHs
    IAN ALEXANDER BLAIR; Fiscal Year: 2010
    ..Successful completion of the proposed research will permit provide a new approach and novel methodology for determining the inter-individual risk factors for DNA damage, which result from environmental exposure to B[a]P. ..
  7. Molecular mechanisms of DNA damage by PAHs
    Ian Blair; Fiscal Year: 2009
    ..Successful completion of the proposed research will permit provide a new approach and novel methodology for determining the inter-individual risk factors for DNA damage, which result from environmental exposure to B[a]P. ..
  8. Molecular mechanisms of DNA damage by PAHs
    Ian Blair; Fiscal Year: 2007
    ..Successful completion of the proposed research will permit provide a new approach and novel methodology for determining the inter-individual risk factors for DNA damage, which result from environmental exposure to B[a]P. ..
  9. DIHYDRODIOL DEHYDROGENASE AND PAH METABOLISM/ACTIVATION
    TREVOR PENNING; Fiscal Year: 1999
    ..PAH o-quinones cause cell death, are frame-shift mutagens, form stable DNA adducts, and act as potent nucleases...
  10. DIHYDRODIOL DEHYDROGENASE AND PAH METABOLISM/ACTIVATION
    TREVOR PENNING; Fiscal Year: 2002
    ..PAH o-quinones cause cell death, are frame-shift mutagens, form stable DNA adducts, and act as potent nucleases...
  11. GENOTOXICITY IN CYP1A2 DEFICIENT TRANSGENIC MICE
    Daniel Nebert; Fiscal Year: 2002
    ..Chronically, ABP causes bladder ABP-DNA adducts and tumor formation as the consequence of hepatic CYP1A2 and N-acetyltransferase (NAT2) levels, as well as ..
  12. DNA cross-linking by diepoxybutane
    Natalia Tretyakova; Fiscal Year: 2009
    ..of mutagenesis and cytotoxicity resulting from BD exposure by providing key information about bifunctional DEB-DNA adducts, including their formation in vivo following exposure to BD, their effects on DNA structure, mispairing ..
  13. GENOTOXICITY IN CYP1A2 DEFICIENT TRANSGENIC MICE
    Daniel Nebert; Fiscal Year: 1999
    ..Chronically, ABP causes bladder ABP-DNA adducts and tumor formation as the consequence of hepatic CYP1A2 and N-acetyltransferase (NAT2) levels, as well as ..
  14. DNA cross-linking by diepoxybutane
    Natalia Y Tretyakova; Fiscal Year: 2010
    ..of mutagenesis and cytotoxicity resulting from BD exposure by providing key information about bifunctional DEB-DNA adducts, including their formation in vivo following exposure to BD, their effects on DNA structure, mispairing ..
  15. GENOTOXICITY IN CYP1A2 DEFICIENT TRANSGENIC MICE
    Daniel Nebert; Fiscal Year: 2000
    ..Chronically, ABP causes bladder ABP-DNA adducts and tumor formation as the consequence of hepatic CYP1A2 and N-acetyltransferase (NAT2) levels, as well as ..
  16. DNA cross-linking by diepoxybutane
    Natalia Y Tretyakova; Fiscal Year: 2011
    ..of mutagenesis and cytotoxicity resulting from BD exposure by providing key information about bifunctional DEB-DNA adducts, including their formation in vivo following exposure to BD, their effects on DNA structure, mispairing ..
  17. GENOTOXICITY IN CYP1A2 DEFICIENT TRANSGENIC MICE
    Daniel Nebert; Fiscal Year: 2001
    ..Chronically, ABP causes bladder ABP-DNA adducts and tumor formation as the consequence of hepatic CYP1A2 and N-acetyltransferase (NAT2) levels, as well as ..
  18. DIHYDRODIOL DEHYDROGENASE AND PAH METABOLISM/ACTIVATION
    TREVOR PENNING; Fiscal Year: 2000
    ..PAH o-quinones cause cell death, are frame-shift mutagens, form stable DNA adducts, and act as potent nucleases...
  19. DIHYDRODIOL DEHYDROGENASE AND PAH METABOLISM/ACTIVATION
    TREVOR PENNING; Fiscal Year: 2000
    ..PAH o-quinones cause cell death, are frame-shift mutagens, form stable DNA adducts, and act as potent nucleases...
  20. CORE--MOLECULAR EPIDEMIOLOGY AND ECOGENETICS
    Margaret Spitz; Fiscal Year: 2001
    ..to carcinogenesis, 2) the evaluation of polymorphisms in select metabolic and DNA repair genes and DNA adducts in the etiology of lung, bladder, breast, and pancreatic cancers, and 3) the development of statistical models ..
  21. DIHYDRODIOL DEHYDROGENASE AND PAH METABOLISM/ACTIVATION
    TREVOR PENNING; Fiscal Year: 2001
    ..PAH o-quinones cause cell death, are frame-shift mutagens, form stable DNA adducts, and act as potent nucleases...
  22. DIHYDRODIOL DEHYDROGENASE AND PAH METABOLISM/ACTIVATION
    TREVOR PENNING; Fiscal Year: 2001
    ..PAH o-quinones cause cell death, are frame-shift mutagens, form stable DNA adducts, and act as potent nucleases...
  23. DIHYDRODIOL DEHYDROGENASE AND PAH METABOLISM/ACTIVATION
    TREVOR PENNING; Fiscal Year: 1999
    ..PAH o-quinones cause cell death, are frame-shift mutagens, form stable DNA adducts, and act as potent nucleases...
  24. REPAIR OF DNA DAMAGED BY MODEL ENVIRONMENTAL CHEMICALS
    ERIC MOON SHONG TANG; Fiscal Year: 1993
    ..dimers (CPD) in the transcribed strand of active genes, however, we have found that the repair of BPDE-DNA adducts shows little, if any, gene-specific and strand-specific repair, and the repair of NAAAF-DNA adducts shows ..
  25. Role of cytochrome P4501B1 in oxygen-mediated pulmonary injury
    Bhagavatula Moorthy; Fiscal Year: 2011
    ..lung injury by inducing CYP1A enzymes;and (iii) exacerbating enhanced formation of ROS-mediated oxidative DNA adducts derived from 8- 5'cyclopurines or lipid peroxidation products, resulting in oxygen-mediated injury...
  26. DNA Adducts of the Carcinogen Acetaldehyde
    Stephen Hecht; Fiscal Year: 2002
    ..In spite of extensive human exposure to this carcinogen, little is known about its DNA adducts, which are likely central to its carcinogenic activity...
  27. DNA Adducts of the Carcinogen Acetaldehyde
    Stephen Hecht; Fiscal Year: 2004
    ..In spite of extensive human exposure to this carcinogen, little is known about its DNA adducts, which are likely central to its carcinogenic activity...
  28. DNA Adducts of the Carcinogen Acetaldehyde
    Stephen Hecht; Fiscal Year: 2003
    ..In spite of extensive human exposure to this carcinogen, little is known about its DNA adducts, which are likely central to its carcinogenic activity...
  29. DNA Adducts of the Carcinogen Acetaldehyde
    Stephen Hecht; Fiscal Year: 2006
    ..In spite of extensive human exposure to this carcinogen, little is known about its DNA adducts, which are likely central to its carcinogenic activity...
  30. DNA Adducts of the Carcinogen Acetaldehyde
    Stephen Hecht; Fiscal Year: 2005
    ..In spite of extensive human exposure to this carcinogen, little is known about its DNA adducts, which are likely central to its carcinogenic activity...
  31. MECHANISMS OF CARCINOGENESIS OF AROMATIC HYDROCARONS
    Ercole Cavalieri; Fiscal Year: 1993
    ..of representative carcinogenic methylbenz[a]anthracenes (MBA), we propose to identify and quantify the DNA adducts of 3- methylcholanthrene (MC), 6-MBA, 7-MBA, 8-MBA and 12-MBA formed in vitro with activation by horseradish ..
  32. Translesion DNA synthesis in humans
    Louise Prakash; Fiscal Year: 2009
    ..DNA lesions to be studied include UV photoproducts, and DNA adducts such as 1,N6-ethenodeoxyadenosine, 1,N2-propano-2'-deoxyguanosine, and (+) trans-dG-N2- benzo[a]pyrene ..
  33. Prophylactic Use of Vitamin E for Prevention of Occupational PAH-Induced Damage
    Debbie Mustacich; Fiscal Year: 2009
    ..Levels of BP-DNA adducts in cigarette smokers are decreased by vitamin E (a-tocopherol) administration...
  34. Prophylactic Use of Vitamin E for Prevention of Occupational PAH-Induced Damage
    Debbie Mustacich; Fiscal Year: 2009
    ..Levels of BP-DNA adducts in cigarette smokers are decreased by vitamin E (a-tocopherol) administration...
  35. Translesion DNA synthesis in humans
    Louise Prakash; Fiscal Year: 2010
    ..DNA lesions to be studied include UV photoproducts, and DNA adducts such as 1,N6-ethenodeoxyadenosine, 1,N2-propano-2'-deoxyguanosine, and (+) trans-dG-N2- benzo[a]pyrene ..
  36. RNA Polymerase Transcription Past DNA Adducts
    David A Scicchitano; Fiscal Year: 2010
    ..We will: (1) investigate RNA polymerase II transcription past select DNA adducts;(2) determine the base sequence of the mRNA produced via bypass of each lesion;and (3) examine DNA repair, ..
  37. DNA ADDUCTS AND APURINIC SITES--THE ESTROGEN-PAH CONNECTION
    Ercole Cavalieri; Fiscal Year: 2000
    ..7,12-dimethylbenz[alpha]anthracene, dibenzo[alpha l)pyrene and some of their metabolites form two types of DNA adducts, stable adducts that remain in DNA or depurinating adducts that are lost from DNA by hydrolysis of the ..
  38. DNA ADDUCTS AND APURINIC SITES--THE ESTROGEN-PAH CONNECTION
    Ercole Cavalieri; Fiscal Year: 1999
    ..7,12-dimethylbenz[alpha]anthracene, dibenzo[alpha l)pyrene and some of their metabolites form two types of DNA adducts, stable adducts that remain in DNA or depurinating adducts that are lost from DNA by hydrolysis of the ..
  39. Mutagenesis of p53 by reactive PAH and ROS
    Jeffrey Field; Fiscal Year: 2009
    ..A critical step in multi-stage carcinogenesis is the mutagenic event that results from the formation of DNA adducts. There are three principle routes of metabolic activation of PAH resulting in the formation of diol- epoxides, ..
  40. USE OF DNA ADDUCTS IN THE STUDY OF PAH CARCINOGENESIS
    Eleanor Rogan; Fiscal Year: 1992
    ..About 90% of all BP-DNA adducts formed are lost from the DNA by depurination. Rodents treated with BP excrete these adducts...
  41. Mutagenesis of p53 by reactive PAH and ROS
    JEFFREY M FIELD; Fiscal Year: 2010
    ..A critical step in multi-stage carcinogenesis is the mutagenic event that results from the formation of DNA adducts. There are three principle routes of metabolic activation of PAH resulting in the formation of diol- epoxides, ..
  42. Mutagenesis of p53 by reactive PAH and ROS
    JEFFREY M FIELD; Fiscal Year: 2010
    ..A critical step in multi-stage carcinogenesis is the mutagenic event that results from the formation of DNA adducts. There are three principle routes of metabolic activation of PAH resulting in the formation of diol- epoxides, ..
  43. AFLATOXIN DNA ADDUCTS DETECTED BY MONOCLONAL ANTIBODIES
    John Groopman; Fiscal Year: 1990
    ..study, we propose to: (1) Produce and characterize monoclonal antibodies to detect the major aflatoxin-DNA adducts: AFB1-N7-Gua and AF-FaPyr. Two strategies for antigen production will be used...
  44. DNA ADDUCTS OF STYRENE AND OTHER VINYL MONOMERS
    Michael Stone; Fiscal Year: 2009
    ..will examine the etiology of butadiene-induced mutagenesis, developing structure-activity relationships for DNA adducts arising from butadiene epoxides and associated with human genotoxicity...
  45. DNA ADDUCTS OF STYRENE AND OTHER VINYL MONOMERS
    Michael Stone; Fiscal Year: 2007
    ..will examine the etiology of butadiene-induced mutagenesis, developing structure-activity relationships for DNA adducts arising from butadiene epoxides and associated with human genotoxicity...
  46. DNA ADDUCTS OF STYRENE AND OTHER VINYL MONOMERS
    Michael P Stone; Fiscal Year: 2010
    ..will examine the etiology of butadiene-induced mutagenesis, developing structure-activity relationships for DNA adducts arising from butadiene epoxides and associated with human genotoxicity...
  47. Isomeric HRT estrogen-DNA adducts: Structure and Repair
    Nicholas Geacintov; Fiscal Year: 2005
    ..is the most significant metabolite among all three equine estrogens, and that it forms unusual cyclic, stable DNA adducts in vitro, in rat mammary tissue model systems, and in human breast tissue...
  48. Isomeric HRT estrogen-DNA adducts: Structure and Repair
    Nicholas Geacintov; Fiscal Year: 2006
    ..is the most significant metabolite among all three equine estrogens, and that it forms unusual cyclic, stable DNA adducts in vitro, in rat mammary tissue model systems, and in human breast tissue...
  49. Isomeric HRT estrogen-DNA adducts: Structure and Repair
    Nicholas Geacintov; Fiscal Year: 2007
    ..is the most significant metabolite among all three equine estrogens, and that it forms unusual cyclic, stable DNA adducts in vitro, in rat mammary tissue model systems, and in human breast tissue...
  50. Isomeric HRT estrogen-DNA adducts: Structure and Repair
    Nicholas Geacintov; Fiscal Year: 2007
    ..is the most significant metabolite among all three equine estrogens, and that it forms unusual cyclic, stable DNA adducts in vitro, in rat mammary tissue model systems, and in human breast tissue...
  51. Isomeric HRT estrogen-DNA adducts: Structure and Repair
    Nicholas Geacintov; Fiscal Year: 2009
    ..is the most significant metabolite among all three equine estrogens, and that it forms unusual cyclic, stable DNA adducts in vitro, in rat mammary tissue model systems, and in human breast tissue...
  52. MOLECULAR MECHANISMS OF HYDROCARBON/DNA INTERACTIONS
    William Baird; Fiscal Year: 1999
    ..are to determine how PAH bind to DNA in mammalian cells and to establish the relationship between specific PAH-DNA adducts and the induction of mutations and cancer...
  53. The role of heterocyclic aromatic amines in breast cancer
    Robert J Turesky; Fiscal Year: 2010
    ..Immunohistochemistry (IHC) and 32P-postlabeling are two principal bioanalytical techniques used to measure DNA adducts, biomarkers of genetic damage...
  54. The role of heterocyclic aromatic amines in breast cancer
    ROBERT TURESKY; Fiscal Year: 2009
    ..Immunohistochemistry (IHC) and 32P-postlabeling are two principal bioanalytical techniques used to measure DNA adducts, biomarkers of genetic damage...
  55. DNA Adducts of the Carcinogen Acetaldehyde
    Stephen Hecht; Fiscal Year: 2009
    ..Levels of acetaldehyde in the environment may increase with the introduction of ethanol-containing fuels. DNA adducts are critical in the carcinogenic process...
  56. DNA Adducts of the Carcinogen Acetaldehyde
    Stephen S Hecht; Fiscal Year: 2010
    ..Levels of acetaldehyde in the environment may increase with the introduction of ethanol-containing fuels. DNA adducts are critical in the carcinogenic process...
  57. DNA Adducts of the Carcinogen Acetaldehyde
    Stephen Hecht; Fiscal Year: 2007
    ..Levels of acetaldehyde in the environment may increase with the introduction of ethanol-containing fuels. DNA adducts are critical in the carcinogenic process...
  58. Sequence Distribution of Tobacco Carcinogen-DNA Adducts
    Natalia Tretyakova; Fiscal Year: 2009
    ..methylation and the local DNA sequence context control the rates of formation and repair of tobacco carcinogen-DNA adducts. Guided by strong preliminary data, this hypothesis will be tested by pursuing the following four specific aims:..
  59. Sequence Distribution of Tobacco Carcinogen-DNA Adducts
    Natalia Y Tretyakova; Fiscal Year: 2010
    ..methylation and the local DNA sequence context control the rates of formation and repair of tobacco carcinogen-DNA adducts. Guided by strong preliminary data, this hypothesis will be tested by pursuing the following four specific aims:..
  60. Sequence Distribution of Tobacco Carcinogen-DNA Adducts
    Natalia Tretyakova; Fiscal Year: 2003
    This project focuses on the distribution of specific tobacco carcinogen-DNA adducts within the two genes frequently targeted in smoking induced lung cancer: the p53 tumor suppressor gene and the K-ras proto-oncogene...
  61. MODULATING BASE EXCISION REPAIR IN TUMOR DRUG RESISTANCE
    Stanton Gerson; Fiscal Year: 2004
    ..such as temozolomide (TMZ) form O6-methylguanine (O6/mG), 7-methylguanine (N7mG), and 3-methyladenine (N3mA) DNA adducts, repaired by at least two mechanisms and a third mechanism that mediates cell death...
  62. Sequence Distribution of Tobacco Carcinogen-DNA Adducts
    Natalia Tretyakova; Fiscal Year: 2006
    This project focuses on the distribution of specific tobacco carcinogen-DNA adducts within the two genes frequently targeted in smoking induced lung cancer: the p53 tumor suppressor gene and the K-ras proto-oncogene...
  63. MODULATING BASE EXCISION REPAIR IN TUMOR DRUG RESISTANCE
    Stanton Gerson; Fiscal Year: 2006
    ..such as temozolomide (TMZ) form O6-methylguanine (O6/mG), 7-methylguanine (N7mG), and 3-methyladenine (N3mA) DNA adducts, repaired by at least two mechanisms and a third mechanism that mediates cell death...
  64. Sequence Distribution of Tabacco Carcinogen-DNA Adducts
    Natalia Tretyakova; Fiscal Year: 2007
    This project focuses on the distribution of specific tobacco carcinogen-DNA adducts within the two genes frequently targeted in smoking induced lung cancer: the p53 tumor suppressor gene and the K-ras proto-oncogene...
  65. MODULATING BASE EXCISION REPAIR IN TUMOR DRUG RESISTANCE
    Stanton Gerson; Fiscal Year: 2005
    ..such as temozolomide (TMZ) form O6-methylguanine (O6/mG), 7-methylguanine (N7mG), and 3-methyladenine (N3mA) DNA adducts, repaired by at least two mechanisms and a third mechanism that mediates cell death...
  66. MECHANISM OF CHROMIUM CARCINOGENICITY
    KAREN WETTERHAHN; Fiscal Year: 1990
    ..2) Chromium (VI)-induced DNA lesions, in the form of DNA strand breaks, DNA cross-links, chromium-DNA adducts and radical-DNA adducts, which result from the attack f "active intermediates" formed during the metabolism of ..
  67. p53-Independent Cell Death Signaling by Mitomycin DNA Adducts
    JILL E BARGONETTI; Fiscal Year: 2010
    ..Our central hypothesis is that DMC provokes p53-independent cell death due to specific DNA adducts formed and that elucidating the pathway(s) will enable efficient drug design parameters...
  68. p53-Independent Cell Death Signaling by Mitomycin DNA Adducts
    Jill Bargonetti; Fiscal Year: 2009
    ..Our central hypothesis is that DMC provokes p53-independent cell death due to specific DNA adducts formed and that elucidating the pathway(s) will enable efficient drug design parameters...
  69. Metabolism and Detoxification of Base Propenals
    Sanjay Srivastava; Fiscal Year: 2005
    ..concentrations BPs are cytotoxic and cytostatic, whereas at low concentrations they form potentially mutagenic DNA adducts. Propenal-derived DNA adducts have been detected in high abundance in normal and cancerous human tissues and it ..
  70. Role of local P450 enzymes in chemical carcinogenesis in mouse mammary gland
    Jun Gu; Fiscal Year: 2010
    ..whether, following systemic exposure to a given procarcinogen, the bulk of the activated carcinogen, or its DNA adducts, in the breast tissue is derived from local metabolism or from the liver;the latter organ is generally much ..
  71. CARCINOGENIC CYCLIC NITROSAMINE DNA ADDUCTS
    Stephen Hecht; Fiscal Year: 2000
    ..We hypothesize that the formation and persistence of specific DNA adducts of NPYR and NPIP are responsible for their organospecific carcinogenic activity in rats...
  72. Bypass of DNA lesions by translesion synthesis in yeast
    Louise Prakash; Fiscal Year: 2009
    ..To elucidate the mechanisms that govern replicative bypass of DNA adducts in eukaryotes including in humans, the proposed studies will utilize the yeast Saccharomyces cerevisiae...
  73. MOLECULAR TOXICOLOGY OF DNA ADDUCTS
    ARTHUR GROLLMAN; Fiscal Year: 1999
    The central theme of this Program Project revolves around the genetic toxicology of DNA adducts. The chemistry and biology of environmental mutagens, including aromatic amines, benzo(a)pyrenes food mutagens, and products of oxidative DNA ..
  74. LC-MS Analysis of DNA Adducts of Aromatic Amines: Relationship to Bladder Cancer
    Paul Vouros; Fiscal Year: 2010
    The covalent binding of genotoxins to DNA to form DNA adducts is considered to be a first step in chemically induced carcinogenesis...
  75. LC-MS Analysis of DNA Adducts of Aromatic Amines: Relationship to Bladder Cancer
    Paul Vouros; Fiscal Year: 2009
    The covalent binding of genotoxins to DNA to form DNA adducts is considered to be a first step in chemically induced carcinogenesis...
  76. Molecular dosimetry of endogeneous genotoxins
    Ian Blair; Fiscal Year: 2007
    ..Aim 4. To quantify endogenous lipid hydroperoxide-derived DNA-adducts, RNA- adducts, GSH-adducts as potential cancer biomarkers in animal models of colon cancer and in human colon tissue. ..
  77. Molecular dosimetry of endogeneous genotoxins
    Ian Blair; Fiscal Year: 2006
    ..Aim 4. To quantify endogenous lipid hydroperoxide-derived DNA-adducts, RNA- adducts, GSH-adducts as potential cancer biomarkers in animal models of colon cancer and in human colon tissue. ..
  78. BINDING OF AROMATIC HYDROCARBONS TO NUCLEIC ACIDS
    Eleanor Rogan; Fiscal Year: 1990
    ..The objectives of this grant are to continue elucidating the benzo(a)pyrene (BP)- DNA adducts formed by one-electron oxidation, to begin investigating their significance, and to examine the hypothesis that ..
  79. Molecular dosimetry of endogeneous genotoxins
    IAN ALEXANDER BLAIR; Fiscal Year: 2010
    ..Aim 4. To quantify endogenous lipid hydroperoxide-derived DNA-adducts, RNA- adducts, GSH-adducts as potential cancer biomarkers in animal models of colon cancer and in human colon tissue. ..
  80. Molecular dosimetry of endogeneous genotoxins
    Ian Blair; Fiscal Year: 2009
    ..Aim 4. To quantify endogenous lipid hydroperoxide-derived DNA-adducts, RNA- adducts, GSH-adducts as potential cancer biomarkers in animal models of colon cancer and in human colon tissue. ..
  81. ULTRASENSITIVE METHODS FOR HUMAN DNA ADDUCT QUANTITATION
    James Swenberg; Fiscal Year: 2000
    b>DNA adducts are believed to be a major source of the mutations involved in carcinogenesis...
  82. HUMAN MISMATCH REPAIR IN CHEMICAL CARCINOGENESIS
    Guo Min Li; Fiscal Year: 1999
    ..Environmental carcinogens covalently modify DNA to form carcinogen-DNA adducts, which induce mutations that initiate carcinogenesis...
  83. Metabolism and Detoxification of Base Propenals
    Sanjay Srivastava; Fiscal Year: 2007
    ..concentrations BPs are cytotoxic and cytostatic, whereas at low concentrations they form potentially mutagenic DNA adducts. Propenal-derived DNA adducts have been detected in high abundance in normal and cancerous human tissues and it ..
  84. CHROMIUM(III) GENOTOXICITY--DIRECT AND INDIRECT PATHWAYS
    DIANE STEARNS; Fiscal Year: 1999
    ..To test whether Cr (III) is responsible for the formation of Cr-DNA adducts, different adducts will be synthesized and examined by MR spectroscopy, and other techniques including X-ray ..
  85. Metabolism and Detoxification of Base Propenals
    Sanjay Srivastava; Fiscal Year: 2006
    ..concentrations BPs are cytotoxic and cytostatic, whereas at low concentrations they form potentially mutagenic DNA adducts. Propenal-derived DNA adducts have been detected in high abundance in normal and cancerous human tissues and it ..
  86. Metabolism and Detoxification of Base Propenals
    Sanjay Srivastava; Fiscal Year: 2004
    ..concentrations BPs are cytotoxic and cytostatic, whereas at low concentrations they form potentially mutagenic DNA adducts. Propenal-derived DNA adducts have been detected in high abundance in normal and cancerous human tissues and it ..
  87. HUMAN MISMATCH REPAIR IN CHEMICAL CARCINOGENESIS
    Guo Min Li; Fiscal Year: 2001
    ..Environmental carcinogens covalently modify DNA to form carcinogen-DNA adducts, which induce mutations that initiate carcinogenesis...
  88. Metabolism and Detoxification of Base Propenals
    Sanjay Srivastava; Fiscal Year: 2003
    ..concentrations BPs are cytotoxic and cytostatic, whereas at low concentrations they form potentially mutagenic DNA adducts. Propenal-derived DNA adducts have been detected in high abundance in normal and cancerous human tissues and it ..
  89. HUMAN MISMATCH REPAIR IN CHEMICAL CARCINOGENESIS
    Guo Min Li; Fiscal Year: 2000
    ..Environmental carcinogens covalently modify DNA to form carcinogen-DNA adducts, which induce mutations that initiate carcinogenesis...
  90. CHROMIUM III GENOTOXICITY--DIRECT AND INDIRECT PATHWAYS
    DIANE STEARNS; Fiscal Year: 1999
    ..To test whether Cr (III) is responsible for the formation of Cr-DNA adducts, different adducts will be synthesized and examined by MR spectroscopy, and other techniques including X-ray ..
  91. CHROMIUM(III) GENOTOXICITY--DIRECT AND INDIRECT PATHWAYS
    DIANE STEARNS; Fiscal Year: 2001
    ..To test whether Cr (III) is responsible for the formation of Cr-DNA adducts, different adducts will be synthesized and examined by MR spectroscopy, and other techniques including X-ray ..