dna adducts

Summary

Summary: Covalent adducts between chemical mutagens and DNA. Such couplings activate DNA repair processes and, unless repaired prior to DNA replication, may lead to nucleotide substitutions, deletions, and chromosome rearrangements. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)

Top Publications

  1. ncbi Molecular mechanisms of mammalian global genome nucleotide excision repair
    Ludovic C J Gillet
    Institute for Molecular Cancer Research, University of Zurich, Switzerland
    Chem Rev 106:253-76. 2006
  2. ncbi Mechanisms of resistance to cisplatin
    M Kartalou
    Division of Bioengineering and Environmental Health and Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Mutat Res 478:23-43. 2001
  3. ncbi XRCC1, XRCC3, XPD gene polymorphisms, smoking and (32)P-DNA adducts in a sample of healthy subjects
    G Matullo
    Dipartimento di Genetica, Biologia e Biochimica, Universita di Torino, 10126, Torino, ISI Foundation, Institute for Scientific Interchange, Villa Gualino, 10133, Torino, Italy
    Carcinogenesis 22:1437-45. 2001
  4. ncbi Doxorubicin-DNA adducts induce a non-topoisomerase II-mediated form of cell death
    Lonnie P Swift
    Department of Biochemistry, La Trobe University, Victoria, Australia
    Cancer Res 66:4863-71. 2006
  5. ncbi Aristolochic acid nephropathy: a worldwide problem
    Frédéric D Debelle
    Department of Nephrology, Dialysis and Renal Transplantation, Erasme Hospital, Brussels, Belgium
    Kidney Int 74:158-69. 2008
  6. pmc Metabolism and biomarkers of heterocyclic aromatic amines in molecular epidemiology studies: lessons learned from aromatic amines
    Robert J Turesky
    Division of Environmental Health Sciences, Wadsworth Center, Albany, New York 12201, United States
    Chem Res Toxicol 24:1169-214. 2011
  7. pmc Prenatal exposure to polycyclic aromatic hydrocarbons, benzo[a]pyrene-DNA adducts, and genomic DNA methylation in cord blood
    Julie B Herbstman
    Department of Environmental Health Sciences, Columbia University Mailman School of Public Health, New York, New York 10032, USA
    Environ Health Perspect 120:733-8. 2012
  8. ncbi Tobacco smoke carcinogens, DNA damage and p53 mutations in smoking-associated cancers
    Gerd P Pfeifer
    Division of Biology, Beckman Research Institute of the City of Hope, Duarte, California, CA 91010, USA
    Oncogene 21:7435-51. 2002
  9. pmc Probing for DNA damage with β-hairpins: similarities in incision efficiencies of bulky DNA adducts by prokaryotic and human nucleotide excision repair systems in vitro
    Yang Liu
    Chemistry Department, New York University, New York, NY 10003, USA
    DNA Repair (Amst) 10:684-96. 2011
  10. ncbi AHR- and DNA-damage-mediated gene expression responses induced by benzo(a)pyrene in human cell lines
    Sarah L Hockley
    Section of Molecular Carcinogenesis, The Institute of Cancer Research, Surrey, UK
    Chem Res Toxicol 20:1797-810. 2007

Detail Information

Publications390 found, 100 shown here

  1. ncbi Molecular mechanisms of mammalian global genome nucleotide excision repair
    Ludovic C J Gillet
    Institute for Molecular Cancer Research, University of Zurich, Switzerland
    Chem Rev 106:253-76. 2006
  2. ncbi Mechanisms of resistance to cisplatin
    M Kartalou
    Division of Bioengineering and Environmental Health and Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Mutat Res 478:23-43. 2001
    ..An improved understanding of the mechanisms of resistance operative in vivo has identified targets for intervention and may increase the utility of cisplatin for the treatment of cancer...
  3. ncbi XRCC1, XRCC3, XPD gene polymorphisms, smoking and (32)P-DNA adducts in a sample of healthy subjects
    G Matullo
    Dipartimento di Genetica, Biologia e Biochimica, Universita di Torino, 10126, Torino, ISI Foundation, Institute for Scientific Interchange, Villa Gualino, 10133, Torino, Italy
    Carcinogenesis 22:1437-45. 2001
    ..These data show that all the analysed polymorphisms could result in deficient DNA repair and suggest a need for further investigation into the possible interactions between these polymorphisms, smoking and other risk factors...
  4. ncbi Doxorubicin-DNA adducts induce a non-topoisomerase II-mediated form of cell death
    Lonnie P Swift
    Department of Biochemistry, La Trobe University, Victoria, Australia
    Cancer Res 66:4863-71. 2006
    ..Here, we show that doxorubicin-DNA adducts (formed by the coadministration of doxorubicin with non-toxic doses of formaldehyde-releasing prodrugs) induce ..
  5. ncbi Aristolochic acid nephropathy: a worldwide problem
    Frédéric D Debelle
    Department of Nephrology, Dialysis and Renal Transplantation, Erasme Hospital, Brussels, Belgium
    Kidney Int 74:158-69. 2008
    ..Finally, despite the Food and Drug Administration's warnings concerning the safety of botanical remedies containing AA, these herbs are still sold via the Internet...
  6. pmc Metabolism and biomarkers of heterocyclic aromatic amines in molecular epidemiology studies: lessons learned from aromatic amines
    Robert J Turesky
    Division of Environmental Health Sciences, Wadsworth Center, Albany, New York 12201, United States
    Chem Res Toxicol 24:1169-214. 2011
    ..Lastly, the analytical approaches that have been successfully employed to biomonitor aromatic amines and HAAs, and emerging biomarkers of HAAs that may be implemented in molecular epidemiology studies are discussed...
  7. pmc Prenatal exposure to polycyclic aromatic hydrocarbons, benzo[a]pyrene-DNA adducts, and genomic DNA methylation in cord blood
    Julie B Herbstman
    Department of Environmental Health Sciences, Columbia University Mailman School of Public Health, New York, New York 10032, USA
    Environ Health Perspect 120:733-8. 2012
    ..These PAH-DNA adducts are established markers of cancer risk...
  8. ncbi Tobacco smoke carcinogens, DNA damage and p53 mutations in smoking-associated cancers
    Gerd P Pfeifer
    Division of Biology, Beckman Research Institute of the City of Hope, Duarte, California, CA 91010, USA
    Oncogene 21:7435-51. 2002
    ..The available data suggest that p53 mutations in lung cancers can be attributed to direct DNA damage from cigarette smoke carcinogens rather than to selection of pre-existing endogenous mutations...
  9. pmc Probing for DNA damage with β-hairpins: similarities in incision efficiencies of bulky DNA adducts by prokaryotic and human nucleotide excision repair systems in vitro
    Yang Liu
    Chemistry Department, New York University, New York, NY 10003, USA
    DNA Repair (Amst) 10:684-96. 2011
    ..These observations suggest that, in addition to recognition involving the initial modified duplexes, downstream events involving UvrC can also play a role in distinguishing and processing different lesions in prokaryotic NER...
  10. ncbi AHR- and DNA-damage-mediated gene expression responses induced by benzo(a)pyrene in human cell lines
    Sarah L Hockley
    Section of Molecular Carcinogenesis, The Institute of Cancer Research, Surrey, UK
    Chem Res Toxicol 20:1797-810. 2007
    ..Promoter analysis identified candidate genes for direct transcriptional regulation by either AHR or p53. These analyses have further dissected and characterized the complex cellular response to BaP...
  11. ncbi XRCC1 polymorphisms: effects on aflatoxin B1-DNA adducts and glycophorin A variant frequency
    R M Lunn
    Laboratory of Computational Biology and Risk Assessment, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Cancer Res 59:2557-61. 1999
    ..AFB1-DNA adducts were measured by ELISA, and erythrocyte GPA variant frequency (NN and NO) was assessed in MN heterozygotes with ..
  12. ncbi Oxidative damage to DNA: formation, measurement and biochemical features
    Jean Cadet
    Laboratoire Lesions des Acides Nucleiques, SCIB DRFMC and FRE 2600, CEA Grenoble, F 38054 Grenoble Cedex 9, France
    Mutat Res 531:5-23. 2003
    ..For this purpose modified oligonucleotides in which cyclopurine, and cyclopyrimidine nucleosides were site-specifically inserted were synthesized...
  13. ncbi Lipid peroxidation-DNA damage by malondialdehyde
    L J Marnett
    A B Hancock Jr Memorial Laboratory for Cancer Research Center in Molecular Toxicology, Vanderbilt Cancer Center, Department of Biochemistry, Vanderbilt University School of Medicine, Nashville TN 37232, USA
    Mutat Res 424:83-95. 1999
    ..High throughput methods for its detection and quantitation will be extremely useful for screening large populations...
  14. ncbi Importance of chromium-DNA adducts in mutagenicity and toxicity of chromium(VI)
    Anatoly Zhitkovich
    Department of Pathology and Laboratory Medicine, Brown University, Box G E507, Providence, Rhode Island 02912, USA
    Chem Res Toxicol 18:3-11. 2005
  15. pmc Polycyclic aromatic hydrocarbons-aromatic DNA adducts in cord blood and behavior scores in New York city children
    Frederica P Perera
    Department of Environmental Health Sciences, Mailman School of Public Health, Columbia University, New York, New York 10032, USA
    Environ Health Perspect 119:1176-81. 2011
    Airborne polycyclic aromatic hydrocarbons (PAH) are widespread urban pollutants that can bind to DNA to form PAH-DNA adducts. Prenatal PAH exposure measured by personal monitoring has been linked to cognitive deficits in childhood in a ..
  16. ncbi Upregulation of clusterin in prostate and DNA damage in spermatozoa from bisphenol A-treated rats and formation of DNA adducts in cultured human prostatic cells
    Silvio De Flora
    Department of Health Sciences, University of Genoa, Genoa, Italy
    Toxicol Sci 122:45-51. 2011
    ..We previously demonstrated that administration of BPA to female mice results in the formation of DNA adducts and proteome alterations in the mammary tissue...
  17. ncbi Mirror image stereoisomers of the major benzo[a]pyrene N2-dG adduct are bypassed by different lesion-bypass DNA polymerases in E. coli
    Kwang Young Seo
    Biology Department, Boston University, 5 Cummington Street, Boston, MA 02215, USA
    DNA Repair (Amst) 5:515-22. 2006
    ....
  18. ncbi In vitro genotoxicity of PAH mixtures and organic extract from urban air particles part II: human cell lines
    O Sevastyanova
    Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic
    Mutat Res 620:123-34. 2007
    ..b>DNA adducts were analyzed by (32)P-postlabeling...
  19. ncbi Lipid peroxidation-induced etheno-DNA adducts in the liver of patients with the genetic metal storage disorders Wilson's disease and primary hemochromatosis
    J Nair
    Division of Toxicology and Cancer Risk Factors, Deutsches Krebsforschungszentrum, Heidelberg, Germany
    Cancer Epidemiol Biomarkers Prev 7:435-40. 1998
    ..Thus, the etheno adducts are implicated as initiating DNA damage in copper/iron-induced carcinogenesis in humans and should also be explored as biomarkers in disease progression and prevention trials...
  20. ncbi Formation of PAH-DNA adducts after in vivo and vitro exposure of rats and lung cells to different commercial carbon blacks
    Paul J A Borm
    Particle Research, Institut für Umweltmedizinische Forschung IUF at the Heinrich Heine University, Dusseldorf, Germany
    Toxicol Appl Pharmacol 205:157-67. 2005
    The current study was designed to test the possible release and bioavailability of polycyclic aromatic hydrocarbons (PAHs) from a set of commercial carbon blacks (CBs) as well as the ability of these PAHs to form bulky DNA adducts.
  21. ncbi Human DNA polymerase kappa encircles DNA: implications for mismatch extension and lesion bypass
    Samer Lone
    Department of Structural and Chemical Biology, Mount Sinai School of Medicine, Box 1677, 1425 Madison Avenue, New York, NY 10029, USA
    Mol Cell 25:601-14. 2007
    ....
  22. ncbi A single amino acid governs enhanced activity of DinB DNA polymerases on damaged templates
    Daniel F Jarosz
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nature 439:225-8. 2006
    ....
  23. ncbi Metabolic activation of polycyclic and heterocyclic aromatic hydrocarbons and DNA damage: a review
    Weiling Xue
    Department of Environmental Health, University of Cincinnati Medical Center, 3223 Eden Avenue, Cincinnati, OH 45267 0056, USA
    Toxicol Appl Pharmacol 206:73-93. 2005
    ..The review emphasizes the chemical/biochemical reactions involved in the metabolic processes and the chemical structures of metabolites and DNA adducts.
  24. ncbi DNA repair gene polymorphisms, bulky DNA adducts in white blood cells and bladder cancer in a case-control study
    G Matullo
    Dipartimento di Genetica, Biologia e Biochimica, Universita di Torino, Turin, Italy
    Int J Cancer 92:562-7. 2001
    ..Results were correlated with DNA damage measured as (32)P-post-labeling bulky DNA adducts in white blood cells from peripheral blood...
  25. pmc Polkappa protects mammalian cells against the lethal and mutagenic effects of benzo[a]pyrene
    Tomoo Ogi
    Laboratories of Gene Information Analysis and Signal Transduction, Institute for Virus Research, Kyoto University, Shogoin Kawara cho 53, Sakyo ku, Japan
    Proc Natl Acad Sci U S A 99:15548-53. 2002
    ..Thus, our results indicate that Polkappa plays an important role in suppressing mutations at DNA lesions generated by B[a]P...
  26. ncbi Repair of O(6)-alkylguanine by alkyltransferases
    A E Pegg
    Departments of Cellular and Molecular Physiology and Pharmacology, Milton S Hershey Medical Center, Pennsylvania State University College of Medicine, P O Box 850, 500 University Drive, Hershey, PA, USA
    Mutat Res 462:83-100. 2000
    ..b>DNA adducts at the O(6)-position of guanine are a major factor in the carcinogenic, mutagenic, apoptopic and clastogenic ..
  27. ncbi DNA adducts from acetaldehyde: implications for alcohol-related carcinogenesis
    Philip J Brooks
    Section on Molecular Neurobiology, Laboratory of Neurogenetics, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, 5625 Fishers Lane, Room 3S32, MSC 9412, Bethesda, MD 20892 9412, USA
    Alcohol 35:187-93. 2005
    ..Inherited variation in the genes encoding the proteins involved in the repair of PdG and its secondary adducts may contribute to susceptibility to alcoholic beverage-related carcinogenesis...
  28. ncbi Polymorphisms in nucleotide excision repair genes, polycyclic aromatic hydrocarbon-DNA adducts, and breast cancer risk
    Katherine D Crew
    Department of Epidemiology, Mailman School of Public Health, Columbia University, 161 Fort Washington Avenue, New York, NY 10032, USA
    Cancer Epidemiol Biomarkers Prev 16:2033-41. 2007
    Genes involved in the nucleotide excision repair (NER) pathway, which removes bulky DNA adducts, are potential low-penetrance cancer susceptibility genes...
  29. pmc Unusual sequence effects on nucleotide excision repair of arylamine lesions: DNA bending/distortion as a primary recognition factor
    Vipin Jain
    Department of Biomedical and Pharmaceutical Sciences, University of Rhode Island, Kingston, RI 02881, USA
    Nucleic Acids Res 41:869-80. 2013
    ..This work represents a novel 3'-next flanking sequence effect as a unique NER factor for bulky arylamine lesions in E. coli...
  30. pmc Exocyclic carbons adjacent to the N6 of adenine are targets for oxidation by the Escherichia coli adaptive response protein AlkB
    Deyu Li
    Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Am Chem Soc 134:8896-901. 2012
    ..The observation that AlkB can demethylate m6A in vitro suggests a role for AlkB in regulation of important cellular functions in vivo...
  31. ncbi Hepatic aflatoxin B1-DNA adducts and TP53 mutations in patients with hepatocellular carcinoma despite low exposure to aflatoxin B1 in southern Japan
    Ken Shirabe
    Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan
    Liver Int 31:1366-72. 2011
    ..Mutations in codon 249, exon 7 are a hallmark of AFB1 exposure. Therefore, to clarify the role of AFB1 in hepatocarcinogenesis, we examined AFB1-DNA in liver tissue and sequenced TP53 in Japanese patients with HCC...
  32. ncbi Formation of acetaldehyde-derived DNA adducts due to alcohol exposure
    Hsu Sheng Yu
    Department of Environmental Health, University of Occupational and Environmental Health, Kitakyushu 807 8555, Japan
    Chem Biol Interact 188:367-75. 2010
    ..Moreover, they support that chronic alcohol consumption contributes to formation of various DNA adducts. Although some DNA adducts formation is demonstrated to be an initiation step of carcinogenesis, it is still ..
  33. ncbi Genotoxic potential of Polycyclic Aromatic Hydrocarbons-coated onto airborne Particulate Matter (PM 2.5) in human lung epithelial A549 cells
    Sylvain Billet
    LCE EA 2598, Toxicologie Industrielle et Environnementale, Universite du Littoral Cote d Opale, Maison de la Recherche en Environnement Industriel de Dunkerque 2, 189A, Avenue Maurice Schumann, 59140 Dunkerque, France
    Cancer Lett 270:144-55. 2008
    ..of Polycyclic Aromatic Hydrocarbons (PAH)-coated onto air pollution PM, and, thereafter, the formation of PAH-DNA adducts in a human lung epithelial cell model (A549 cell line). Cells were exposed to Dunkerque city's PM(2...
  34. ncbi Resveratrol, a natural aryl hydrocarbon receptor antagonist, protects lung from DNA damage and apoptosis caused by benzo[a]pyrene
    Ariel Revel
    Division of Reproductive Sciences, Department of Obstetrics and Gynecology, University of Toronto, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 21X5, Canada
    J Appl Toxicol 23:255-61. 2003
    ..in the aetiology of lung cancer through the induction of benzo[a]pyrene diol epoxidation (BPDE) and BPDE-DNA adducts. Because BaP metabolization requires cytochrome P-450 1A1 (CYP1A1) induction through activation of the AhR, we ..
  35. ncbi Efficiency and accuracy of SOS-induced DNA polymerases replicating benzo[a]pyrene-7,8-diol 9,10-epoxide A and G adducts
    Xuan Shen
    Department of Biological Sciences and Chemistry, Hedco Molecular Biology Laboratory, University of Southern California, Los Angeles, California 90089 1340, USA
    J Biol Chem 277:5265-74. 2002
    ..SOS polymerases are adept at copying a variety of lesions, but the relative contribution of each SOS polymerase to copying damaged DNA appears to be determined by the lesion's identity...
  36. ncbi PAH-DNA adducts in environmentally exposed population in relation to metabolic and DNA repair gene polymorphisms
    Blanka Binkova
    Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine AS CR and Health Institute of Central Bohemia, Videnska 1083, 14220 Prague, Czech Republic
    Mutat Res 620:49-61. 2007
    ..This study investigates relationships between exposure to PAHs, polymorphisms of these genes and DNA adducts in group of occupationally exposed policemen (EXP, N=53, males, aged 22-50 years) working outdoors in the ..
  37. pmc Alcohol metabolism in human cells causes DNA damage and activates the Fanconi anemia-breast cancer susceptibility (FA-BRCA) DNA damage response network
    Jessy Abraham
    Section on Molecular Neurobiology, Laboratory of Neurogenetics, National Institute on Alcohol Abuse and Alcoholism, NIH, Rockville, Maryland, USA
    Alcohol Clin Exp Res 35:2113-20. 2011
    ..We recently reported that exposure of human cells in vitro to acetaldehyde resulted in the activation of the Fanconi anemia-breast cancer susceptibility (FA-BRCA) DNA damage response network...
  38. pmc The efficiencies of damage recognition and excision correlate with duplex destabilization induced by acetylaminofluorene adducts in human nucleotide excision repair
    Jung Eun Yeo
    Department of Chemistry, Stony Brook University, Stony Brook, NY 11794, USA
    Chem Res Toxicol 25:2462-8. 2012
    ..Our study is the first systematic analysis correlating these three parameters and supports the idea that initial damage recognition by XPC-RAD23B is a key rate-limiting step in NER...
  39. ncbi Nucleotide misincorporation, 3'-mismatch extension, and responses to abasic sites and DNA adducts by the polymerase component of bacterial DNA ligase D
    Lyudmila Yakovleva
    Molecular Biology Program, Sloan Kettering Institute, New York, New York 10021, USA
    J Biol Chem 281:25026-40. 2006
    ..Covalent benzo[a]pyrene-dG and benzo[c]phenanthrene-dA adducts in the template strand are durable roadblocks to POL elongation. POL can slowly insert a dNMP opposite the adduct, but is impaired in the subsequent extension step...
  40. ncbi The Raf kinase inhibitor BAY 43-9006 reduces cellular uptake of platinum compounds and cytotoxicity in human colorectal carcinoma cell lines
    Martina Heim
    Department of Internal Medicine and Medical Oncology, West German Cancer Centre, University Medical School of Essen, Essen, Germany
    Anticancer Drugs 16:129-36. 2005
    ..spectrometry, BAY significantly reduced cellular uptake of platinum compounds and thereby the generation of DNA adducts. Taken together, co-incubation with BAY results in reduced cellular uptake of platinum compounds and ..
  41. ncbi Synthesis of the C8-deoxyguanosine adduct of the food mutagen IQ
    Z Wang
    Department of Chemistry, Vanderbilt University, VU Station B 351822, Nashville, Tennessee 37235-1822, USA
    Org Lett 3:565-8. 2001
    ..The key step is a palladium-catalyzed N-arylation of a suitably protected 8-bromo-2'-deoxyguanosine derivative...
  42. ncbi Human biomonitoring: state of the art
    Jurgen Angerer
    Institute and Outpatient Clinic of Occupational, Social and Environmental Medicine, University of Erlangen Nuremberg, Schillerstrasse 25 29, 91054 Erlangen, Germany
    Int J Hyg Environ Health 210:201-28. 2007
    ..Protein adducts, especially Hb-adducts, as surrogates of DNA adducts measuring exposure as well as biochemical effect very specifically and sensitively are a still better means to ..
  43. ncbi Influence of the metabolic properties of human cells on the kinetic of formation of the major benzo[a]pyrene DNA adducts
    Caroline Marie
    Laboratoire des Lésions des Acides Nucléiques, LCIB UMR E 3 CEA UJF, DRFMC, CEA Grenoble, 17 Avenue des Martyrs 38054 Grenoble Cedex 9, France
    J Appl Toxicol 28:579-90. 2008
    ..Some of them, including benzo[a]pyrene (B[a]P), are tumorigenic due to their ability to generate DNA adducts. In order to define potential biomarkers of B[a]P exposure, the aim of the study was to identify the major ..
  44. pmc Elevated levels of the pro-carcinogenic adduct, O(6)-methylguanine, in normal DNA from the cancer prone regions of the large bowel
    A C Povey
    Cancer Research Campaign Section of Genome Damage and Repair, Paterson Institute for Cancer Research, Manchester, M20 9BX, UK
    Gut 47:362-5. 2000
    ..The biological significance of this exposure is, as yet, unknown but in situ NOC formation is bacterially catalysed suggesting that NOC formation and potentially DNA alkylation will vary throughout the large bowel...
  45. pmc Structures of 2-acetylaminofluorene modified DNA revisited: insight into conformational heterogeneity
    Satyakam Patnaik
    Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, Rhode Island 02881, USA
    Chem Res Toxicol 23:1650-2. 2010
    ..The S/B/W-conformational heterogeneities were found to be sensitive to the nature of the flanking DNA sequence contexts and pH...
  46. ncbi Ochratoxin a causes DNA damage and cytogenetic effects but no DNA adducts in rats
    Angela Mally
    Department of Toxicology, University of Wurzburg, Germany
    Chem Res Toxicol 18:1253-61. 2005
    ..Oxidative DNA damage as a mechanism of OTA-dependent DNA damage is consistent with the absence of lipophilic DNA adducts as assessed by 32P-postlabeling analysis...
  47. pmc Resistance of bulky DNA lesions to nucleotide excision repair can result from extensive aromatic lesion-base stacking interactions
    Dara A Reeves
    Department of Chemistry, Department of Biology, New York University, New York, NY 10003, USA
    Nucleic Acids Res 39:8752-64. 2011
    ....
  48. pmc The vital role of polymerase ζ and REV1 in mutagenic, but not correct, DNA synthesis across benzo[a]pyrene-dG and recruitment of polymerase ζ by REV1 to replication-stalled site
    Keiji Hashimoto
    Department of Pharmacological Sciences, State University of New York, Stony Brook, New York 11794 8651, USA
    J Biol Chem 287:9613-22. 2012
    ..This indicates that a non-Y family polymerase(s) can insert a nucleotide opposite BPDE-dG, but the subsequent extension from miscoding termini depends on REV1-polζ in a RAD18-dependent manner...
  49. pmc Use of LC-MS/MS and stable isotopes to differentiate hydroxymethyl and methyl DNA adducts from formaldehyde and nitrosodimethylamine
    Kun Lu
    Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Chem Res Toxicol 25:664-75. 2012
    Formaldehyde is a known human and animal carcinogen that forms DNA adducts, and causes mutations. While there is widespread exposure to formaldehyde in the environment, formaldehyde is also an essential biochemical in all living cells...
  50. ncbi Probing the conformational heterogeneity of the acetylaminofluorene-modified 2'-deoxyguanosine and DNA by 19F NMR spectroscopy
    B P Cho
    Department of Biomedical Sciences, College of Pharmacy, University of Rhode Island, Kingston 02881, USA
    Biochemistry 38:7572-83. 1999
    ..et al. (1997) J. Am. Chem. Soc. 119, 5384-5389]. The exclusive stacked nature of the AAF adducts may provide insight into why AAF adducts are more mutagenic and prone to repair than the nonacetylated AF adducts...
  51. pmc Chemistry and structural biology of DNA damage and biological consequences
    Michael P Stone
    Department of Chemistry, Center in Molecular Toxicology, Vanderbilt Ingram Cancer Center, Vanderbilt University, Nashville, TN 37235, USA
    Chem Biodivers 8:1571-615. 2011
    ..The structural analysis of various DNA adducts reveals that conformational and chemical rearrangements and interconversions are a common theme...
  52. pmc Site-specific radical formation in DNA induced by Cu(II)-H₂O₂ oxidizing system, using ESR, immuno-spin trapping, LC-MS, and MS/MS
    Suchandra Bhattacharjee
    Laboratory of Toxicology and Pharmacology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA
    Free Radic Biol Med 50:1536-45. 2011
    ..The DMPO adduct formed is initially EPR active but subsequently is oxidized to the stable nitrone, which can then be detected by IST and further characterized by MS and MS/MS...
  53. ncbi Processing of anthracycline-DNA adducts via DNA replication and interstrand crosslink repair pathways
    R A Bilardi
    Department of Biochemistry, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, Victoria 3086, Australia
    Biochem Pharmacol 83:1241-50. 2012
    ..of topoisomerase II, however many anthracyclines, including doxorubicin, are also capable of forming drug-DNA adducts. Anthracycline-DNA adducts present an unusual obstacle for cells as they are covalently attached to one DNA ..
  54. pmc Influence of flanking sequence context on the conformational flexibility of aminofluorene-modified dG adduct in dA mismatch DNA duplexes
    Nidhi Jain
    Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, RI 02881, USA
    Nucleic Acids Res 37:1628-37. 2009
    ....
  55. ncbi Differential removal of DNA adducts derived from anti-diol epoxides of dibenzo[a,l]pyrene and benzo[a]pyrene in human cells
    Kristian Dreij
    The Institute of Environmental Medicine, Division of Biochemical Toxicology, Karolinska Institutet, Box 210, S 17177 Stockholm, Sweden
    Chem Res Toxicol 18:655-64. 2005
    ..of their ultimate carcinogenic diol epoxide (DE) intermediates, differences in structural features of the DNA adducts, and differences in DNA adduct recognition and the subsequent lesion removal by nucleotide excision repair (NER)..
  56. ncbi The pyrrolobenzodiazepine dimer SJG-136 forms sequence-dependent intrastrand DNA cross-links and monoalkylated adducts in addition to interstrand cross-links
    Khondaker M Rahman
    Gene Targeted Drug Design Research Group, The School of Pharmacy, University of London, 29 39 Brunswick Square, London WC1N 1AX, UK
    J Am Chem Soc 131:13756-66. 2009
    ....
  57. ncbi Standardization and validation of DNA adduct postlabelling methods: report of interlaboratory trials and production of recommended protocols
    D H Phillips
    Institute of Cancer Research, Haddow Laboratories, Sutton, Surrey, UK
    Mutagenesis 14:301-15. 1999
    ..was to devise and test improved protocols of the 32P-postlabelling assay for the detection of carcinogen-DNA adducts. The intention was to reverse the drift of different investigators using increasingly divergent experimental ..
  58. ncbi The 32P-postlabeling assay for DNA adducts
    David H Phillips
    Institute of Cancer Research, Brookes Lawley Building, Cotswold Road, Sutton, Surrey SM2 5NG, UK
    Nat Protoc 2:2772-81. 2007
    32P-postlabeling analysis is an ultrasensitive method for the detection and quantitation of carcinogen-DNA adducts. It consists of four principal steps: (i) enzymatic digestion of DNA to nucleoside 3'-monophosphates; (ii) enrichment of ..
  59. ncbi Validation of biomarkers for the study of environmental carcinogens: a review
    Valentina Gallo
    Department of Epidemiology and Public Health, Imperial College London, London, UK
    Biomarkers 13:505-34. 2008
    ..Our aim is to review published work on the validation of selected biomarkers: bulky DNA adducts, N-nitroso compounds, 1-hydroxypyrene, and oxidative damage to DNA...
  60. pmc Adduct-specific monoclonal antibodies for the measurement of cisplatin-induced DNA lesions in individual cell nuclei
    Bernd Liedert
    Institut fur Zellbiologie, Universitatsklinikum, Essen Germany
    Nucleic Acids Res 34:e47. 2006
    ..we describe a new method to establish and characterize monoclonal antibodies (Mab) for structurally defined DNA adducts. The two major reaction products of cisplatin, the guanine-guanine (Pt-[GG]) and adenine-guanine (Pt-[AG]) ..
  61. ncbi Carcinogenic polycyclic aromatic hydrocarbon-DNA adducts and mechanism of action
    William M Baird
    Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, Oregon 97331, USA
    Environ Mol Mutagen 45:106-14. 2005
    ..Thus, PAHs may also act through a promotional mechanism in addition to serving as tumor initiators. Previous studies on these mechanisms are described and summarized...
  62. pmc Molecular dosimetry of N2-hydroxymethyl-dG DNA adducts in rats exposed to formaldehyde
    Kun Lu
    Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill, North Carolina 27599, United States
    Chem Res Toxicol 24:159-61. 2011
    ..Our data clearly demonstrated that exogenous formaldehyde DNA adducts form in a highly nonlinear fashion, with a 21...
  63. ncbi Cisplatin: from DNA damage to cancer chemotherapy
    S M Cohen
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge 02139, USA
    Prog Nucleic Acid Res Mol Biol 67:93-130. 2001
    ..Continued efforts to understand the mechanism of genotoxicity of cisplatin are expected to lead to the discovery of new drugs and combinations for the improvement of cancer chemotherapy...
  64. pmc Acrolein is a major cigarette-related lung cancer agent: Preferential binding at p53 mutational hotspots and inhibition of DNA repair
    Zhaohui Feng
    Department of Environmental Medicine, Pathology, and Medicine, New York University School of Medicine, Tuxedo, NY 10987, USA
    Proc Natl Acad Sci U S A 103:15404-9. 2006
    ..Acrolein (Acr) is abundant in CS, and it can directly adduct DNA. Acr-DNA adducts, similar to PAH-DNA adducts, induce predominantly G-to-T transversions in human cells...
  65. ncbi Overexpression of cMOAT (MRP2/ABCC2) is associated with decreased formation of platinum-DNA adducts and decreased G2-arrest in melanoma cells resistant to cisplatin
    Bernd Liedert
    Institute of Cell Biology, University of Essen, Germany
    J Invest Dermatol 121:172-6. 2003
    ..Kinetics of formation and elimination of platinum-DNA adducts suggest that the DNA repair capacity for Pt-d(GpG) adducts was not elevated in platinum drug-resistant melanoma ..
  66. ncbi 5-Azacytidine induced methyltransferase-DNA adducts block DNA replication in vivo
    H Kenny Kuo
    Department of Biochemistry, Duke University, Durham, NC 27710, USA
    Cancer Res 67:8248-54. 2007
    ..These results imply that aza-C-induced MTase-DNA adducts block DNA replication in vivo. RecA-dependent X structures were also observed after aza-C treatment...
  67. pmc Intra-hepatic arterial administration with miriplatin suspended in an oily lymphographic agent inhibits the growth of tumors implanted in rat livers by inducing platinum-DNA adducts to form and massive apoptosis
    Mitsuharu Hanada
    Pharmacology Research Laboratories, Dainippon Sumitomo Pharma Co, Ltd, Osaka, Japan
    Cancer Chemother Pharmacol 64:473-83. 2009
    ..We clarified the usefulness of miriplatin as an agent for transarterial chemoembolization...
  68. pmc Site-specific incorporation of N-(deoxyguanosin-8-yl)-2-acetylaminofluorene (dG-AAF) into oligonucleotides using modified 'ultra-mild' DNA synthesis
    Ludovic C J Gillet
    Institute for Molecular Cancer Research, University of Zürich August Forel Strasse 7, 8008 Zurich, Switzerland
    Nucleic Acids Res 33:1961-9. 2005
    ..Our synthetic approach should facilitate comprehensive studies of the mechanisms of repair and mutagenesis induced by dG-AAF adducts in DNA and should be of general use for the incorporation of base-labile functionalities into DNA...
  69. pmc The sequence dependence of human nucleotide excision repair efficiencies of benzo[a]pyrene-derived DNA lesions: insights into the structural factors that favor dual incisions
    Konstantin Kropachev
    Department of Chemistry, New York University, New York, NY 10003, USA
    J Mol Biol 386:1193-203. 2009
    Nucleotide excision repair (NER) is a vital cellular defense system against carcinogen-DNA adducts, which, if not repaired, can initiate cancer development...
  70. ncbi Arsenite and its biomethylated metabolites interfere with the formation and repair of stable BPDE-induced DNA adducts in human cells and impair XPAzf and Fpg
    Tanja Schwerdtle
    Institute of Food Chemistry and Toxicology, University of Karlsruhe, Postfach 6980, D 76128, Karlsruhe, Germany
    DNA Repair (Amst) 2:1449-63. 2003
    ..Altogether the results suggest that besides arsenite, especially the trivalent methylated metabolites may contribute to diminished NER at low concentrations...
  71. ncbi Adverse reproductive outcomes from exposure to environmental mutagens
    R J Sram
    Laboratory of Genetic Ecotoxicology, Regional Institute of Hygiene of Central Bohemia, c o Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20, Prague, Czech Republic
    Mutat Res 428:203-15. 1999
    ..Genotoxicity of urban air particles <10 microm (PM10) in in vitro system was determined by the analysis of DNA adducts. The highest DNA binding activity was observed in aromatic fraction, identifying DNA adducts of carcinogenic ..
  72. pmc Endogenous versus exogenous DNA adducts: their role in carcinogenesis, epidemiology, and risk assessment
    James A Swenberg
    Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Toxicol Sci 120:S130-45. 2011
    ..known human carcinogens": formaldehyde, vinyl chloride (VC), and ethylene oxide (EO), each of which forms DNA adducts identical to endogenous DNA adducts in all individuals...
  73. ncbi A critical evaluation of DNA adducts as biological markers for human exposure to polycyclic aromatic compounds
    Roger W L Godschalk
    Department of Health Risk Analysis and Toxicology, University of Maastricht, 6200 MD Maastricht, The Netherlands
    J Biochem Mol Biol 36:1-11. 2003
    ..The successful applicability of DNA adducts as early endpoints depends on several criteria: i...
  74. pmc Acrolein: sources, metabolism, and biomolecular interactions relevant to human health and disease
    Jan F Stevens
    Department of Pharmaceutical Sciences, Oregon State University, Corvallis, OR, USA
    Mol Nutr Food Res 52:7-25. 2008
    ..Acrolein adduction disrupts the function of these biomacromolecules which may result in mutations, altered gene transcription, and modulation of apoptosis...
  75. ncbi AlkB reverses etheno DNA lesions caused by lipid oxidation in vitro and in vivo
    James C Delaney
    Biological Engineering Division, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nat Struct Mol Biol 12:855-60. 2005
    ..These reactions show a previously unrecognized chemical versatility of AlkB. In mammals, the corresponding AlkB homologs may defend against aging, cancer and oxidative stress...
  76. ncbi Associations of plasma aflatoxin B1-albumin adduct level with plasma selenium level and genetic polymorphisms of glutathione S-transferase M1 and T1
    S Y Chen
    Columbia University School of Public Health, New York, NY 10032, USA
    Nutr Cancer 38:179-85. 2000
    ..This study provides insight into the dietary and genetic factors influencing AFB1-albumin adduct formation in an isolated population with high liver cancer mortality...
  77. ncbi DNA adduct formation in precision-cut rat liver and lung slices exposed to benzo[a]pyrene
    Jeanine A Harrigan
    Department of Pharmacology and Toxicology, State University of New York at Buffalo, Buffalo, New York 14214, USA
    Toxicol Sci 77:307-14. 2004
    Chemical-DNA adducts provide an integrated measure of exposure, absorption, bioactivation, detoxification, and DNA repair following exposure to a genotoxic agent...
  78. pmc Multiple ATR-Chk1 pathway proteins preferentially associate with checkpoint-inducing DNA substrates
    Seçil Yilmaz
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina, United States of America
    PLoS ONE 6:e22986. 2011
    ..We conclude that the association and accumulation of multiple checkpoint proteins with DNA structures indicative of DNA damage and replication stress likely contribute to optimal ATR-Chk1 DNA damage checkpoint responses...
  79. ncbi [Effects of CYP1A1 and GSTM1 gene polymorphisms and BPDE-DNA adducts on lung cancer]
    Chun Mei Chen
    Laboratory of Environment Epigenetics, Institute of Environmental Health, College of Public Health, Zhejiang University Medical School, Hangzhou, Zhejiang 310058, P R China
    Zhonghua Yi Xue Yi Chuan Xue Za Zhi 29:23-7. 2012
    To investigate the effect of CYP1A1 and GSTM1 genetic polymorphisms and BPDE-DNA adducts on lung tumorigenesis.
  80. ncbi Functional effect of polymorphisms in 15q25 locus on CHRNA5 mRNA, bulky DNA adducts and TP53 mutations
    Xavier Tekpli
    Section for Toxicology, Department of Biological and Chemical Working Environment, National Institute of Occupational Health, Oslo, Norway
    Int J Cancer 132:1811-20. 2013
    ..were associated with differential levels of genetic alterations measured as the levels of hydrophobic DNA adducts in the adjacent histologically normal tissue of the lung cancer patients and as TP53 mutations in their lung ..
  81. pmc Role of human CYP1A1 and NAT2 in 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine-induced mutagenicity and DNA adducts
    J Bendaly
    Department of Pharmacology and Toxicology, James Graham Brown Cancer Center, and Center for Environmental Genomics and Integrative Biology, University of Louisville School of Medicine, Louisville, KY, USA
    Xenobiotica 39:399-406. 2009
    ..05) following one-way analysis of variance. These results strongly support activation of PhIP by CYP1A1 with little effect of human NAT2 genetic polymorphism on mutagenesis and DNA damage...
  82. ncbi DNA adducts with antioxidant flavonoids: morin, apigenin, and naringin
    Shohreh Nafisi
    Department of Chemistry, Azad University, Central Tehran Branch IAUCTB, Tehran, Iran
    DNA Cell Biol 27:433-42. 2008
    ..10 x 10(3) M(-1). The affinity of ligand-DNA binding is in the order of apigenin > morin > naringin. DNA aggregation and a partial B- to A-DNA transition occurs upon morin, apigenin, and naringin complexation...
  83. ncbi The effect of dibenzo[a,1]pyrene and benzo[a]pyrene on human diploid lung fibroblasts: the induction of DNA adducts, expression of p53 and p21(WAF1) proteins and cell cycle distribution
    B Binkova
    Laboratory of Genetic Ecotoxicology, Regional Institute of Hygiene of Central Bohemia, c o Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic
    Mutat Res 471:57-70. 2000
    ..The induction of p53 and p21(WAF1) protein occurred subsequent to the induction of DNA adducts. The DNA adduct levels correlated with both p53 (R=0.832, P<0.001 and R=0.859, P<0...
  84. ncbi Identification of three major DNA adducts formed by the carcinogenic air pollutant 3-nitrobenzanthrone in rat lung at the C8 and N2 position of guanine and at the N6 position of adenine
    Volker M Arlt
    Institute of Cancer Research, Section of Molecular Carcinogenesis, Sutton, Surrey, United Kingdom
    Int J Cancer 118:2139-46. 2006
    ..Previously, we detected the formation of 3-NBA-derived DNA adducts in rodent tissues by 32P-postlabeling, all of which are derived from reductive metabolites of 3-NBA bound to ..
  85. pmc Differences in conformational dynamics of [Pt3(HPTAB)]6+-DNA adducts with various cross-linking modes
    Yanyan Zhu
    College of Chemistry, Beijing Normal University, Beijing 100875, P R China
    Nucleic Acids Res 37:5930-42. 2009
    ..dynamics simulations and DNA conformational dynamics for a series of trinuclear platinum [Pt(3)(HPTAB)](6+)-DNA adducts [HPTAB = N,N,N',N',N'',N''-hexakis (2-pyridyl-methyl)-1,3,5-tris(aminomethyl) benzene], including three types ..
  86. ncbi DNA adducts in skin biopsies and 1-hydroxypyrene in urine of psoriasis patients and healthy volunteers following treatment with coal tar
    J H J Roelofzen
    Department of Dermatology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Toxicol Lett 213:39-44. 2012
    ..In this study, levels of urinary 1-hydroxypyrene and PAH-DNA adducts in the skin were studied in psoriatic patients and healthy volunteers...
  87. pmc DNA adducts of decarbamoyl mitomycin C efficiently kill cells without wild-type p53 resulting from proteasome-mediated degradation of checkpoint protein 1
    Ernest K Boamah
    Department of Biological Sciences, Hunter College and the Graduate Center, City University of New York, 695 Park Avenue, New York, NY 10065, USA
    Chem Res Toxicol 23:1151-62. 2010
    ..the level of total ubiquitinated proteins without increasing proteasome activity, suggesting that DMC mediated DNA adducts facilitate signal transduction to a pathway targeting cellular proteins for proteolysis...
  88. ncbi Breast cancer chemoprevention: risk-benefit effects of the antioestrogen tamoxifen
    Karen Brown
    Cancer Biomarkers and Prevention Group, The Biocentre, University of Leicester, University Road, Leicester, LE1 7RH, UK
    Expert Opin Drug Saf 1:253-67. 2002
    ....
  89. ncbi DNA adducts in tumour, normal peripheral lung and bronchus, and peripheral blood lymphocytes from smoking and non-smoking lung cancer patients: correlations between tissues and detection by 32P-postlabelling and immunoassay
    Erika Gyorffy
    National Institute of Environmental Health, Jozsef Fodor National Center for Public Health, Budapest, H 1097 Hungary
    Carcinogenesis 25:1201-9. 2004
    Smoking is a major risk factor for lung cancer. This comparative study of smoking-related carcinogen-DNA adducts in pulmonary tissues and peripheral blood lymphocytes aims to further explore the primary DNA damaging processes by ..
  90. ncbi DNA interstrand crosslinks: natural and drug-induced DNA adducts that induce unique cellular responses
    ORLANDO D SCHARER
    Institute of Molecular Cancer Research, University of Zurich, August Forel Strasse 7, 8008 Zurich, Switzerland
    Chembiochem 6:27-32. 2005
  91. ncbi Modulation of gene expression and DNA adduct formation in HepG2 cells by polycyclic aromatic hydrocarbons with different carcinogenic potencies
    Yvonne C M Staal
    Department of Health Risk Analysis and Toxicology, Maastricht University, The Netherlands
    Carcinogenesis 27:646-55. 2006
    ..Our data indicate that discrimination of high and low carcinogenic PAHs by gene expression profiling is feasible. Also, the carcinogenic PAHs induce several pathways that were not affected by the least carcinogenic PAHs...
  92. ncbi Bulky DNA adducts, 4-aminobiphenyl-haemoglobin adducts and diet in the European Prospective Investigation into Cancer and Nutrition (EPIC) prospective study
    Marco Peluso
    Cancer Risk Factor Branch, Analytical and Biomolecular Cytology Unit, CSPO Scientific Institute of Tuscany, Florence, Italy
    Br J Nutr 100:489-95. 2008
    ..and Nutrition (EPIC) cohort the association between dietary items (food groups and nutrients) and aromatic DNA adducts and 4-aminobiphenyl-Hb adducts...
  93. ncbi Mutagenic events in Escherichia coli and mammalian cells generated in response to acetylaminofluorene-derived DNA adducts positioned in the Nar I restriction enzyme site
    Xingzhi Tan
    Laboratory of Chemical Biology, Department of Pharmacological Sciences, State University of New York at Stony Brook, Stony Brook, New York 11794 8651, USA
    Biochemistry 41:14255-62. 2002
    ..3-21%, the higher value at G(2). We conclude from this study that the mutation potential of dG-AAF and dG-AF depends on the structure of the adduct, the sequence context of the lesion, and the host cell used for the experiment...
  94. ncbi Dynamic conformational heterogeneities of carcinogen-DNA adducts and their mutagenic relevance
    Bongsup P Cho
    Department of Biomedical and Pharmaceutical Sciences, University of Rhode Island, Kingston, RI 02882, USA
    J Environ Sci Health C Environ Carcinog Ecotoxicol Rev 22:57-90. 2004
    ..Results from such experiments should provide an accurate account of how a single chemically homogenous adduct gives rise to complex multiple mutations, the earliest step in the induction of cancer...
  95. ncbi Interactive effects of nrf2 genotype and oltipraz on benzo[a]pyrene-DNA adducts and tumor yield in mice
    Minerva Ramos-Gomez
    Department of Environmental Health Sciences, Bloomberg School of Public Health, The Johns Hopkins University, Baltimore, MD 21202, USA
    Carcinogenesis 24:461-7. 2003
    ..To evaluate the role of nrf2 genotype on B[a]P disposition, levels of B[a]P-DNA adducts were measured as tetrols released from DNA isolated from target (forestomach) and non-target tissues (liver) of ..
  96. pmc cis-Diammine(pyridine)chloroplatinum(II), a monofunctional platinum(II) antitumor agent: Uptake, structure, function, and prospects
    Katherine S Lovejoy
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 105:8902-7. 2008
    ..cDPCP-DNA adducts are removed by the nucleotide excision repair apparatus, albeit much less efficiently than bifunctional ..
  97. ncbi Aromatic DNA adducts and risk of gastrointestinal cancers: a case-cohort study within the EPIC-Spain
    Antonio Agudo
    Unit of Nutrition, Environment and Cancer, Cancer Epidemiology Research Program, Catalan Institute of Oncology ICO, Av Gran Vía 199 203, L Hospitalet de Llobregat, Spain
    Cancer Epidemiol Biomarkers Prev 21:685-92. 2012
    ..Activated metabolites of these compounds may bind to DNA forming bulky adducts...
  98. pmc Mutagenesis and repair induced by the DNA advanced glycation end product N2-1-(carboxyethyl)-2'-deoxyguanosine in human cells
    Daniel Tamae
    Division of Molecular Medicine, Beckman Research Institute of the City of Hope, 1500 East Duarte Road, Duarte, California 91010, United States
    Biochemistry 50:2321-9. 2011
    ..0001). This suggested the involvement of an NER-dependent mutagenic process in response to critical levels of DNA damage, possibly mediated by error-prone Y-family polymerases...
  99. ncbi Benzo[a]pyrene-7,8-quinone-3'-mononucleotide adduct standards for 32P postlabeling analyses: detection of benzo[a]pyrene-7,8-quinone-calf thymus DNA adducts
    Narayanan Balu
    Environmental Carcinogenesis Division, Office of Research and Development, U S Environmental Protection Agency, Research Triangle Park, NC 27711, USA
    Anal Biochem 355:213-23. 2006
    ..Chem. Res. Toxicol. 17 (2004) 827-838). However, the identification of BPQ-DNA adducts by 32P postlabeling methods from in vitro and in vivo exposures required 3'-monophosphate derivatives of BPQ-dG,..
  100. ncbi DNA adducts and oxidative DNA damage induced by organic extracts from PM2.5 in an acellular assay
    Jan Topinka
    Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague 4, Czech Republic
    Toxicol Lett 202:186-92. 2011
    ..8-oxodG, levels) induced by EOMs in an acellular assay of calf thymus DNA coupled with ³²P-postlabeling (DNA adducts) and ELISA (8-oxodG) in the presence and absence of microsomal S9 fraction were employed...
  101. pmc Repair of laser-localized DNA interstrand cross-links in G1 phase mammalian cells
    Parameswary A Muniandy
    Laboratory of Molecular Gerontology, NIA, National Institutes of Health, Baltimore, Maryland 21224, USA
    J Biol Chem 284:27908-17. 2009
    ..DDB2 accumulation at sites of cross-link repair is a marker for the start of the second repair cycle...

Research Grants70

  1. Polycyclic aromatic hydrocarbons and birth outcomes in Mexico City
    MARIE SYLVIA O'NEILL; Fiscal Year: 2013
    ..We will obtain biomarkers relevant to PAH exposure (DNA adducts) in blood samples provided by participants monthly during their pregnancies, and umbilical cord blood obtained ..
  2. Enterococcus faecalis, Colorectal Cancer, and Bystander Effects
    Mark M Huycke; Fiscal Year: 2012
    ..The generation of mutagenic exocyclic DNA adducts and DNA double strand breaks (DSBs) in target cells by the macrophage-induced BSE will be measured using LC/MS/..
  3. MOLECULAR MECHANISMS OF COMPLEX MIXTURE TOXICITY
    Alvaro Puga; Fiscal Year: 2013
    ..polymerase II to the Cyp1a1 promoter, block BaP-inducible gene expression and stimulate the formation of BPDE-DNA adducts. Similarly, sustained exposure to low doses of chromium leads to gradual changes in histone marks and signal ..
  4. CHEMISTRY AND BIOLOGY OF PLATINUM ANTICANCER DRUGS
    Stephen J Lippard; Fiscal Year: 2013
    ..Nucleosomes, the building blocks of chromosomes, will be constructed with site-specific Pt-DNA adducts and structurally characterized in solution by footprinting and in the solid state by X-ray crystallography...
  5. Functional roles of Nrf2 and NQO1 genetic variants in hyperoxic lung injury-ARDS
    Bhagavatula Moorthy; Fiscal Year: 2013
    ..or controls will be analyzed for the presence of SNPs, F2- isoprostanes/isofurans, levels of bulky oxidative DNA adducts, and protein oxidation products...
  6. Molecular mechanisms of cytochrome P4501A regulation by hyperoxia
    Bhagavatula Moorthy; Fiscal Year: 2010
    ..that CYP1A1 enzymes play a protective role against cell toxicity in cultured cells, and that oxidative DNA adducts play a mechanistic role in contributing to oxygen mediated toxicities leading to apoptosis and/or necrosis...
  7. Prevention of Esophageal Cancer with Berries
    Gary Stoner; Fiscal Year: 2013
    ..the effects of dietary anthocyanins and PEITC, alone and in combination, on the formation of NMBA-induced DNA adducts in rat esophagus as well as their effects on the expression of genes associated with cell proliferation, ..
  8. DNA Repair of Endogenous Lesions in Carcinogenesis
    Rabindra Roy; Fiscal Year: 2010
    ..repair of endogenous DNA damage, including oxidized bases (Reactive Oxygen Species (ROS)-induced) and cyclic DNA adducts (enal-induced), plays a critical role in carcinogenesis...
  9. Lipid peroxidation product induced DNA damage in the p53 gene and liver cancer
    ERIC MOON SHONG M TANG; Fiscal Year: 2010
    ..We will determine how 4-HNE-DNA adducts in the p53 gene are processed, and the role of the p53 gene in aldehyde-mediated inhibition of DNA repair in ..
  10. Cancer induction in the ovary by an environmental pollutant, dibenzo[a,l]pyrene
    Kun Ming Chen; Fiscal Year: 2013
    ..Presence of DNA adducts in tissues exposed to environmental carcinogens is crucial to the initiation of the carcinogenic process, and ..
  11. SPLENIC TOXICITY OF ANILINE
    M Firoze Khan; Fiscal Year: 2010
    ..Aim 2 will examine the role of oxidative DNA damage and repair, malondialdehyde-DNA adducts, DNA methylation, and mutations in the tumor suppressor gene p53 in aniline-induced splenic toxicity...
  12. Genomic profiling of yeast resistance to AFB1, a P450-activated carcinogen
    MICHAEL THOMAS FASULLO; Fiscal Year: 2013
    ..resistance and sensitivity, and provide insights into how recombinational repair processes actively tolerate DNA adducts. The strain collections will be made available to the scientific community and will be a valuable resource for ..
  13. Detoxification of Electrophilic Chemical Threat Agents by Nucleophilic Scavengers
    Michael C MacLeod; Fiscal Year: 2010
    ..The primary strategies to be developed in this Project will block the ability of SNMs to form DNA adducts in exposed cells...
  14. RNA Polymerase Transcription Past DNA Adducts
    David A Scicchitano; Fiscal Year: 2013
    ..We will: (1) investigate RNA polymerase II transcription past select DNA adducts;(2) determine the base sequence of the mRNA produced via bypass of each lesion;and (3) examine DNA repair, ..
  15. Development of Novel Agents Targeting Genome Stability and Maintenance for Treati
    John J Turchi; Fiscal Year: 2013
    ..Cisplatin and carboplatin both impart their chemotherapeutic effect by the formation of Pt-DNA adducts which block DNA replication and transcription culminating in apoptosis...
  16. 2-Amino-9H-pyrido[2,3-b]indole: a potential colorectal carcinogen formed in tobac
    Robert J Turesky; Fiscal Year: 2013
    ..In Aim 2, the genotoxicity of AaC in the colorectum of C57BL/6N mice will be determined, by measurement of DNA adducts and aberrant crypt foci, as early biomarkers of neoplasia...
  17. Chemoprevention by black raspberry in oral cancer induced by DB[a,l]P in mice
    KARAM E EL-BAYOUMY; Fiscal Year: 2013
    ..induced by DB[a,l]P and its diol epoxide are very similar, consistent with the formation of stable covalent DNA adducts (Preliminary Results)...
  18. Role of cytochrome P4501B1 in oxygen-mediated pulmonary injury
    Bhagavatula Moorthy; Fiscal Year: 2013
    ..lung injury by inducing CYP1A enzymes;and (iii) exacerbating enhanced formation of ROS-mediated oxidative DNA adducts derived from 8- 5'cyclopurines or lipid peroxidation products, resulting in oxygen-mediated injury...
  19. ELECTROCATALYTIC STUDIES OF TOXIC POLLUTANT ACTIVATION
    James F Rusling; Fiscal Year: 2013
    ..Similar films on nanoparticles provide samples for LC-MS analysis of metabolite-DNA adducts. These approaches will also provide enzyme specificities, site specificities of DNA damage, and pathways of DNA ..
  20. Intra- and Extra-Chromosomal Probes for Mutagenesis by Carcinogens
    John M Essigmann; Fiscal Year: 2013
    ..Two key features of the project are (1) its emphasis on in vivo analysis of the biology of DNA adducts, many of which are produced by human carcinogens and (2) the emphasis on rigorous synthetic methodology for ..
  21. Aflatoxin B1 hepatocarcinogenesis in the mGSTA3-/- mouse
    Stewart Sell; Fiscal Year: 2013
    ..of loss of the mGSTA3 on AFB1-induced toxicity, by measuring the steps of AFB1 metabolism, the levels of AFB1-DNA adducts and extent of oxidative damage in liver tissues...
  22. INFIDELITY OF CYTOSINE METHYLATION AND HUMAN CANCER
    Lawrence C Sowers; Fiscal Year: 2013
    ..that reactive molecules generated by activated neutrophils and eosinophils can generated an array of DNA adducts. Among these products are 5-chlorocytosine (5ClC) and 5-bromocytosine (5BrC)...
  23. Aldo-Keto Reductases and PAH Metabolism/Activation
    Trevor M Penning; Fiscal Year: 2013
    ..Thus, in addition to PAH diol-epoxide-DNA adducts, oxidative DNA lesions may contribute to lung cancer initiation...
  24. Synthesis, StructUre and Replication of Carcirogen-Modified Oligonucleotides
    Carmelo J Rizzo; Fiscal Year: 2013
    ..HCA-DNA adducts have been detected from human tissue after exposure to dietary relevant doses...
  25. Reactive oxygen species and Genomic Instability in CML
    Martin Sattler; Fiscal Year: 2012
    ..Reactive oxygen species (ROS) have already been shown to generate DNA adducts and cause oxidative DNA damage, which can result in genomic mutations...
  26. NYU Lung Cancer Biomarker Center
    William N Rom; Fiscal Year: 2013
    ..lung cancer from high-risk controls with L, Showe (Wistar, UPenn), M-s Tang and colleagues demonstrated DNA adducts occurred at hot spot mutation sites in p53 and K-ras, and that poor repair contributed to adduct persistence...
  27. DNA cross-linking by diepoxybutane
    Natalia Y Tretyakova; Fiscal Year: 2013
    ..of mutagenesis and cytotoxicity resulting from BD exposure by providing key information about bifunctional DEB-DNA adducts, including their formation in vivo following exposure to BD, their effects on DNA structure, mispairing ..
  28. The role of Mc1r in melanocytic UV-induced DNA damage and repair responses
    JOHN A DORAZIO; Fiscal Year: 2013
    ..Southwestern blotting, hOGG1-adapted Comet assay, TBARS assay, and direct quantification of oxidative DNA adducts by GC/MS)...
  29. MOLECULAR EPIDEMIOLOGY OF LUNG CANCER
    Chu Chen; Fiscal Year: 2010
    ..effect is the DNA damage produced by the generation of reactive oxygen species and the formation of DNA adducts. Since the majority of persons who smoke tobacco do not develop lung cancer, it is possible that those who do ..
  30. Sequence Distribution of Tobacco Carcinogen-DNA Adducts
    Natalia Y Tretyakova; Fiscal Year: 2012
    ..methylation and the local DNA sequence context control the rates of formation and repair of tobacco carcinogen-DNA adducts. Guided by strong preliminary data, this hypothesis will be tested by pursuing the following four specific aims:..
  31. In vivo detection and genome-wide location analysis of DNA-adducts
    Stephen B Howell; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): DNA adducts are the hallmark and most common form of DNA damage in the cell...
  32. Pharmacokinetics and Metabolism of Oxidized Curcumin
    Claus Schneider; Fiscal Year: 2013
    ..shown that these metabolites contribute to the regulation of cellular antioxidant response and form covalent DNA adducts. The goal of this grant application is to characterize pharmacokinetics, tissue distribution, and metabolism of ..
  33. Heat Shock Protein-Induced Protection Against Cisplatin-Induced Hair Cell Death
    TIFFANY GRAY BAKER; Fiscal Year: 2013
    ..Cisplatin causes cell death by forming DNA adducts and increasing the pool of reactive oxygen species (ROS) in cells...
  34. Breast Cancer Chemoprevention Strategies
    Ramesh C Gupta; Fiscal Year: 2010
    ..Chromatographic similarity with reference oxidative adducts suggests that tissue DNA adducts originated from free radical-mediation...
  35. Mutagenesis of p53 by reactive PAH and ROS
    JEFFREY M FIELD; Fiscal Year: 2012
    ..A critical step in multi-stage carcinogenesis is the mutagenic event that results from the formation of DNA adducts. There are three principle routes of metabolic activation of PAH resulting in the formation of diol- epoxides, ..
  36. Biomarkers of toxic response to low nicotine cigarette smoke
    Andrew Strasser; Fiscal Year: 2012
    ..that have been used are urinary isoprostanes to monitor oxidative stress, and the analysis of B[a]P-derived DNA adducts in leukocytes...
  37. A Novel Carcinogen-Induced Cell Cycle Checkpoint
    Cyrus Vaziri; Fiscal Year: 2013
    ..application is to elucidate the roles of an E3 ubiquitin ligase termed 'Rad18'in tolerance of bulky DNA adducts (such as B[a]P- and UV-induced lesions) via Trans-Lesion Synthesis (TLS) and in repair of Double Strand Break (..
  38. Mechanisms of selective excision and oxidative repair of alkylated DNA
    Manel Camps; Fiscal Year: 2013
    ..Our studies have at least three direct clinical implications. First, etheno-DNA adducts likely play a role in the etiology of cancer associated with chronic inflammation, and thus results on eA ..
  39. Chemical markers of heterocyclic aromatic amines for human biomonitoring
    Robert J Turesky; Fiscal Year: 2011
    ..will establish noninvasive chemical markers of HAAs, by measuring HAAs in hair, blood protein adducts, and DNA adducts in long-lived white blood cells, as surrogate biomarkers for target sites of cancer risk in humans who ..
  40. Mechanisms for Benzo(a)pyrene-Induced Colon Cancer Exacerbation by Dietary Fat
    Aramandla Ramesh; Fiscal Year: 2013
    ..A linked hypothesis is that B(a)P effects are secondary to production of epoxide or quinones some of which form DNA adducts that cause genotoxicity...
  41. New biomonitoring methodologies to measure DNA adducts in human tissues
    Robert J Turesky; Fiscal Year: 2013
    ..by applicant): The objective of this proposal is to develop new biomonitoring methodologies designed to measure DNA adducts in tissues of humans exposed to chemical carcinogens...
  42. Aromatic Amine-DNA Lesions: Mutagenicity and Repair
    Suse Broyde; Fiscal Year: 2013
    ..Among the plethora of DNA adducts, it is essential to identify the most hazardous ones for purposes of biomonitoring and assessing the exposure ..
  43. Molecular mechanism of cytochrome P4501A1 expression.
    Bhagavatula Moorthy; Fiscal Year: 2013
    ..carcinogenesis, and that a CYP1A2-dependent MC metabolite(s) contributes to the formation of sequence- specific DNA adducts on the regulatory regions of the CYP1A1 promoter [e.g...
  44. Targeting the ERCC1/XPF nuclease for cancer therapy
    John J Turchi; Fiscal Year: 2012
    ..Both cisplatin and carboplatin impart their chemotherapeutic effect by the formation of intrastrand Pt-DNA adducts. These adducts are repaired by the nucleotide excision repair (NER) pathway which relies on the ERCC1/XPF ..
  45. Prophylactic Use of Vitamin E for Prevention of Occupational PAH-Induced Damage
    Debbie Mustacich; Fiscal Year: 2009
    ..Levels of BP-DNA adducts in cigarette smokers are decreased by vitamin E (a-tocopherol) administration...
  46. Coordinating Translesion DNA Synthesis Opposite Damaged DNA
    Robert L Eoff; Fiscal Year: 2013
    ..These so-called replicative DNA polymerases are often less able to bypass DNA adducts. Other specialized polymerases possess lesion bypass abilities that can aid the replication fork when it ..
  47. Targeting nucleotide excision repair in combination cancer therapy
    John J Turchi; Fiscal Year: 2013
    ..Pt agents impart their therapeutic effect by the formation of Pt-DNA adducts, which block DNA replication and transcription...
  48. Bypass of DNA lesions by translesion synthesis in yeast
    Louise Prakash; Fiscal Year: 2013
    ..To elucidate the mechanisms that govern replicative bypass of DNA adducts in eukaryotes including in humans, the proposed studies will utilize the yeast Saccharomyces cerevisiae...
  49. The role of heterocyclic aromatic amines in breast cancer
    Robert J Turesky; Fiscal Year: 2010
    ..Immunohistochemistry (IHC) and 32P-postlabeling are two principal bioanalytical techniques used to measure DNA adducts, biomarkers of genetic damage...
  50. Excision of Carcinogen-DNA Adducts in Nucleosomes
    Nicholas E Geacintov; Fiscal Year: 2013
    ..remarkable differences in the relative excision efficiencies of different, stereochemically well defined PAH-DNA adducts by the human nucleotide excision repair (NER) system in whole cell extracts...
  51. Translesion DNA synthesis in humans
    Louise Prakash; Fiscal Year: 2012
    ..DNA lesions to be studied include UV photoproducts, and DNA adducts such as 1,N6-ethenodeoxyadenosine, 1,N2-propano-2'-deoxyguanosine, and (+) trans-dG-N2- benzo[a]pyrene ..
  52. Polymerase Interactions with Carcinogen-modified DNA
    F Peter Guengerich; Fiscal Year: 2013
    ..solfataricus, Dpo1, Dpo2, Dpo3, and Dpo4, including their roles in normal replication and past several DNA adducts, as well as defining the role of the heterotrimeric PCNA protein in this organism...
  53. Molecular dosimetry of endogeneous genotoxins
    IAN ALEXANDER BLAIR; Fiscal Year: 2010
    ..Aim 4. To quantify endogenous lipid hydroperoxide-derived DNA-adducts, RNA- adducts, GSH-adducts as potential cancer biomarkers in animal models of colon cancer and in human colon tissue. ..
  54. Carcinogenic Metabolites Formed from Antiestrogen
    Judy L Bolton; Fiscal Year: 2013
    ..Initial model studies with deoxynucleosides and DNA will allow characterization of stable DNA adducts, analysis of depurinating adducts, as well as determination of DNA oxidation...
  55. Measurements of BPDE-DNA adducts by solid state nonopore and deep sequencing (PQ
    Farhad Kosari; Fiscal Year: 2013
    Measurements of BPDE-DNA adducts by solid state nanopore and deep sequencing (PQ3) Potent carcinogen Benzo[a]pyrene (BaP) is found in heavily polluted air, smoked food, and tobacco smoke...
  56. Investigating the Role of a Lifestyle Intervention on Novel Estrogen Biomarkers
    Kerryn W Reding; Fiscal Year: 2011
    ..within the catechol estrogen (CE) pathway, in particular hydroxy estrogen (HE) quinones, are capable of forming DNA adducts which may lead in certain instances to DNA mutations...
  57. Roles of the Smc5-Smc6 Holocomplex in Genome Stability
    Michael N Boddy; Fiscal Year: 2013
    ..Smc5-Smc6 in (i) promoting meiotic chromosome segregation at the MI division, (ii) the processing of protein-DNA adducts in a pathway parallel to Tdp1, and (iii) the resolution of DNA structures arising during the restart of ..
  58. Carcinogen-DNA Adducts: Topology, Conformation and Repair (Renewal)
    Suse Broyde; Fiscal Year: 2013
    ..However, it has recently been shown that several of the bulky DNA adducts that they produce after metabolic activation to diol epoxides, are resistant to nucleotide excision repair (NER)..
  59. Polycyclic Aromatic Hydrocarbons And Male Reproductive Health
    HUEIWANG ANNA COOK JENG; Fiscal Year: 2010
    ..Semen will be collected to assess sperm quality and DNA integrity, and PAH-DNA adducts. Features of this design include efficient control for confounding factors, accurate exposure ascertainment, ..
  60. DNA Repair Capacity and Risk of Pre-malignant Lesion in Lung Epithelium
    Hua Zhao; Fiscal Year: 2009
    ..ROS-caused base damages are mainly repaired by base excision repair (BER) and nucleotide excision repair (NER);DNA adducts caused by MPO- related carcinogen activation are repaired by NER...
  61. Curcumin inhbits BPDE-induced damage by lowering the threshold of p53 activation
    ERICA NICOLE ROGERS; Fiscal Year: 2011
    ..Because global genomic repair (GGR) removes most BPDE-DNA adducts in a p53-dependent manner, Specific aim 2 will use immunoslot blot, [32P]-postlabeling, and UvrABC mediated ..
  62. Role of HDAC6 in platinum resistance of non-small cell lung cancer
    XIAOHONG MARY ZHANG; Fiscal Year: 2013
    ..Given the fact that MSH2 serves as a sensor for cisplatin-induced DNA adducts and loss of MSH2 causes cisplatin resistance, we hypothesize that down-regulation of MSH2 protein level and its ..
  63. GENOTOXICITY IN CYP1A2 DEFICIENT TRANSGENIC MICE
    Daniel Nebert; Fiscal Year: 2002
    ..Chronically, ABP causes bladder ABP-DNA adducts and tumor formation as the consequence of hepatic CYP1A2 and N-acetyltransferase (NAT2) levels, as well as ..
  64. AFLATOXIN DNA ADDUCTS DETECTED BY MONOCLONAL ANTIBODIES
    John Groopman; Fiscal Year: 1992
    ..study, we propose to: (1) Produce and characterize monoclonal antibodies to detect the major aflatoxin-DNA adducts: AFB1-N7-Gua and AF-FaPyr. Two strategies for antigen production will be used...
  65. Isomeric HRT estrogen-DNA adducts: Structure and Repair
    Nicholas Geacintov; Fiscal Year: 2009
    ..is the most significant metabolite among all three equine estrogens, and that it forms unusual cyclic, stable DNA adducts in vitro, in rat mammary tissue model systems, and in human breast tissue...
  66. MOLECULAR MECHANISMS OF HYDROCARBON/DNA INTERACTIONS
    William Baird; Fiscal Year: 1999
    ..are to determine how PAH bind to DNA in mammalian cells and to establish the relationship between specific PAH-DNA adducts and the induction of mutations and cancer...
  67. MECHANISM OF CHROMIUM CARCINOGENICITY
    KAREN WETTERHAHN; Fiscal Year: 1993
    ..2) Chromium (VI)-induced DNA lesions, in the form of DNA strand breaks, DNA cross-links, chromium-DNA adducts and radical-DNA adducts, which result from the attack f "active intermediates" formed during the metabolism of ..
  68. Metabolism and Detoxification of Base Propenals
    Sanjay Srivastava; Fiscal Year: 2007
    ..concentrations BPs are cytotoxic and cytostatic, whereas at low concentrations they form potentially mutagenic DNA adducts. Propenal-derived DNA adducts have been detected in high abundance in normal and cancerous human tissues and it ..
  69. HUMAN MISMATCH REPAIR IN CHEMICAL CARCINOGENESIS
    Guo Min Li; Fiscal Year: 2001
    ..Environmental carcinogens covalently modify DNA to form carcinogen-DNA adducts, which induce mutations that initiate carcinogenesis...
  70. ULTRASENSITIVE METHODS FOR HUMAN DNA ADDUCT QUANTITATION
    James Swenberg; Fiscal Year: 2000
    b>DNA adducts are believed to be a major source of the mutations involved in carcinogenesis...